LOCUS       DASTFV010000001         3201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13 k141_5827,
            whole genome shotgun sequence.
ACCESSION   DASTFV010000001 DASTFV010000000
VERSION     DASTFV010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5827"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VFP02_00005"
     CDS             complement(<1..947)
                     /locus_tag="VFP02_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HET9541431.1"
                     /translation="MGWADDAVRAPAIVSLDGNRRIGVAGPGPWADAVARALVVQITA
                     RAGPTAWVLSVPAEPAWRFARWLPHRQGSGAALSVGHDGAGPGIEVADHRAQLTDRCA
                     AVLAWSPDGARLWAAGDGDGRPVRPFLASVELAEDYGRASARWTDPDPASATLPDRLG
                     LASLLTEAAPASEEGRRRLAAPLGVGADGVVTVDLVVDGPHALVAGTTGAGKSELLRT
                     WVTALAHRHPPTAVAFVLVDYKGGSAFDACARLPHVTGVVTDLDAGLGERLLVGLRAE
                     IREREAGLRDAGVGDLRDLVDGPPRLVVVVDEFATLAAEL"
     gene            complement(1232..1906)
                     /locus_tag="VFP02_00010"
     CDS             complement(1232..1906)
                     /locus_tag="VFP02_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9541432.1"
                     /translation="MADGGGGPDLEIRFTPGADVTAADLVEAWPDRPDGMLFVDGAAC
                     AERAPLAACGLADGATVAVGPPAPAALHDLHVTAGPLTPRSVSLPGRVLIGRGVACGL
                     RIDHPSIAAAHALVESVAGGWTVSAAAIGATTALDGEPVGAPTPLRPGAVLSVGAVDL
                     QLHVPTRPALVPPPRGPVHRAASTAAPIPDPPPAPPERPAPPPPPSPPGPASLLLPIA
                     VGVILA"
     gene            complement(2007..2306)
                     /locus_tag="VFP02_00015"
     CDS             complement(2007..2306)
                     /locus_tag="VFP02_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541433.1"
                     /translation="MPFDQVGADLGQLATLRRLFERDAGELERLIQEISAAVGPPGAQ
                     GTVRWEGRVADRFRQDWNQTFAPGLRRLTEALRESATYVERNRQNISQALNGTAV"
     gene            2371..>3201
                     /locus_tag="VFP02_00020"
     CDS             2371..>3201
                     /locus_tag="VFP02_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541434.1"
                     /translation="MVTPQSASGPVPKAGPGLVGINLTTAGHLADALDRVAVEVEGQA
                     REARSAGLEGGQVPIATARLALVARWAAHEAAALRRIIERLRAVEVTGVARWSGGRSP
                     AFADPVAGYAWGLELVDALLAGRMAEARRLLADHGRDPVVATVVVEGLGAAGTVDLLR
                     PAVVGWARPGTDADDQRAVVLGVAGLLAGAERSGTTDLTVGDLADAAGRGSVPLRALA
                     LLFAGGARFPSSFVRDAVRTVVAPLNAALRAEPGLGVGPWMIPAAGAPLDARVVVLAA
                     A"
BASE COUNT          384 a         1160 c         1282 g          375 t
ORIGIN      
        1 agctcggcgg ccagcgtggc gaactcgtcc accacgacca ccagccgcgg cgggccgtcg
       61 acgaggtccc ggaggtcgcc cacccccgcg tcgcgcaggc cggcctccct ttcccggatc
      121 tcggcccgca ggccgacgag caaccgctcg cccaacccgg cgtcgagatc ggtgacgacc
      181 cccgtcacgt ggggcagccg ggcgcaggca tcgaaggcgc tgccgccctt gtagtccacg
      241 aggacgaagg ccacggccgt cggcgggtgg cggtgggcca gagccgtgac ccaggtccgc
      301 aggagctcgc tcttgcccgc acctgtcgtg ccggccacga gcgcgtgggg gccgtcgacg
      361 accaggtcca ccgtgacgac gccgtcggcg ccgacgccca acggcgcggc gaggcgtcgc
      421 cgcccctcct cgctggcggg cgcagcttcg gtcaggagcg acgccagccc gagccgatcg
      481 ggcagggtgg cgctcgccgg gtcgggatcg gtccaccggg ccgacgcccg cccgtagtcc
      541 tcggcgagct cgaccgaggc gaggaagggg cggaccggtc gtccgtcgcc gtcgcctgcg
      601 gcccacaggc gggcgccgtc ggggctccac gccaggacgg ccgcgcagcg gtcggtcagc
      661 tgcgcccggt gatcggccac ttcgatgccg ggaccggcgc cgtcgtggcc gacgctcagg
      721 gcggcgcccg acccctggcg gtgggggagc catcgggcga atcgccaggc cggctcggcc
      781 ggaacgctca ggacccaggc tgtggggccg gcgcgggccg tgatctggac gacgagggcc
      841 cgggccacgg cgtcggccca cgggcccggc ccggccaccc cgatccggcg gttgccgtcg
      901 aggctcacga tcgccggggc ccgcaccgcg tcgtcggccc agcccacggc gacctgtcga
      961 atcgtgggat cgccttcccg gacggcccac aacccgacgc cggagcgggc ttgggcgagg
     1021 gcctcgacgg gggtcgggaa gcgagtccgc tcgcgggcgg cgaggccgtg atggtgcgtc
     1081 gccagctgct gccggtgtcg gtcgacgtcc tcggcccgct cggcgtcgtc gcgtcgcagg
     1141 aggcgacggt gacgacgccg ggcgtcgagg tgggcgccta cggccaggat cggggtcgcc
     1201 gcggtgacca ggccggcggc cgggtggacg gtcaggccag gatcacgccc acggcgatcg
     1261 gcaagagcag tgaggcgggg cccggcgggc tcgggggcgg gggcggggcc ggccgctccg
     1321 gtggcgccgg cggtggatcc gggatagggg cggccgtgga tgccgcccga tggaccggcc
     1381 ctcggggtgg gggcaccagg gcgggacggg tcgggacgtg gagctggagg tccacggcgc
     1441 cgaccgacag cacggcgccc ggccggaggg gggtgggggc gcccacgggc tcgccgtcca
     1501 gggccgtggt tgccccgatg gcggccgcgc tgaccgtcca cccacccgcg accgactcga
     1561 cgagcgcgtg agcggcagca atcgaggggt gatcgatgcg gagcccgcag gcgacgcctc
     1621 gcccgatgag gacacggccc gggagcgaca ccgagcgcgg ggtgagggga ccggctgtca
     1681 cgtggaggtc atggagggcg gcaggggccg gagggcccac ggccaccgtc gctccatcgg
     1741 ccaacccgca ggcggccagt ggggcccgct cggcgcaggc cgccccgtcc acgaacagca
     1801 tcccgtcggg tcggtcgggc caggcctcga ccaggtccgc ggcggtgacg tcggcaccgg
     1861 gggtgaaccg gatctcgagg tccggccccc cgccgccgtc ggccacacgg acgatgaccg
     1921 acggcgacga gaggtcgggg ttggatcggg ggcccggccc acagggaggg ggcgggcagg
     1981 cagcaggcag ggcccctcga tccagatcag accgccgtgc cgttgagggc ctggctgatg
     2041 ttctggcggt tgcgctcgac gtaggtcgcg gattcccgca gggcttcggt gaggcgccgc
     2101 agccccggcg cgaaggtctg gttccagtcc tgccggaagc ggtcggccac ccggccctcc
     2161 cagcgcacgg tgccctgggc gcccgggggc ccgacggcgg cggagatctc ctggatcagg
     2221 cgttccagct cgccggcgtc ccgctcgaag aggcggcgca gcgtggccag ctgccccaga
     2281 tcggctccca cctgatcgaa cggcatggcg gcgttccctt cacatcgcgg ctgttgacca
     2341 tgagcattac atgaaacaac atgaaacaca atggttacgc cgcaaagtgc ttctggcccc
     2401 gtcccgaagg ccggccccgg cctggtcggc atcaacctga cgacggccgg tcacctggcc
     2461 gacgccctcg accgagtggc cgtggaggtc gagggccagg cgcgggaggc ccgctcggcc
     2521 ggcctcgagg gcggccaggt cccgatcgcc accgcccgac tggccctcgt ggctcgctgg
     2581 gccgcgcacg aggcggcggc cctccgacgg atcatcgagc ggttgcgcgc cgtcgaggtc
     2641 acgggcgtcg cccgctggag cgggggccgg agcccggcgt tcgccgaccc ggtggcgggg
     2701 tacgcgtggg gcctcgagct ggtcgatgct ctgctcgccg gtcggatggc ggaagcccgg
     2761 cgcctgctgg ccgaccatgg ccgtgatccc gtggtggcca ccgtcgtggt cgagggcctc
     2821 ggcgcggcgg gcacggtgga cctcctccgg ccggccgtcg tggggtgggc ccggcccggg
     2881 accgacgccg acgaccagcg cgcggtcgtg ctcggggtcg ccggcctcct ggccggggcc
     2941 gagcggagcg ggacgaccga tctcacggtc ggtgatctgg ccgacgccgc cggccgaggg
     3001 tcggttccgc tgcgcgcgct agccctcctg ttcgcgggcg gggcccgatt cccgagctcg
     3061 ttcgtgcgag atgccgtccg aacggtggtc gcgccgctca acgccgccct ccgcgccgag
     3121 cccggcctcg gcgtcgggcc gtggatgatc ccggcggcgg gtgcgcctct cgatgcccgt
     3181 gtcgtggtcc tggcggccgc g
//
LOCUS       DASTFV010000002         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13 k141_7031,
            whole genome shotgun sequence.
ACCESSION   DASTFV010000002 DASTFV010000000
VERSION     DASTFV010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7031"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1184)
                     /locus_tag="VFP02_00025"
     CDS             complement(180..1184)
                     /locus_tag="VFP02_00025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET9541435.1"
                     /translation="MDPRAPLADIGAHARRVEALGFDALHVAETTNDAFLTCALALEH
                     TSTLVVRPALALAFVRSPMQVAYATWSLAGFAPGRFQLGLGTQIKPNIVGRFGVEWAD
                     PVDRMADYVAAVRAVFTAFQEGGPLRHEGPHYRLTRLQPEFNPGPLDGGPVPIWLGGV
                     NRRACRLAGAVGDGYVTHPTNSDRRYLSELCLPALEQGLTEAGRDRSDVQVVVGGMVA
                     TGRDDAAVAEARARSRYMLGFLFSTPAYRRTLELHGWEDLGDRLRSMTKAGRWEDLEA
                     TVTDDVLDTVVAAGRYDELARVLGDRYGDLADGVLLHAPIDPADDEPFAAAVEELQAV
                     "
     gene            complement(1311..2636)
                     /locus_tag="VFP02_00030"
     CDS             complement(1311..2636)
                     /locus_tag="VFP02_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET9541436.1"
                     /translation="MTDVEDRPDLTDDAPVGSTIETGPLGPARIPASRYTSSAWAASE
                     AERVWPRTWLIAAARGQVAEPGDWCEFTCGSLSVLIVRGDDGELRAFQNVCLHRGSEL
                     CQGEGEGRTEIRCGYHRWAWNLRGELREIPSRKGFGPLRNDDLPLLPVRVDTWGELVF
                     VNLDREAEPLADWLEGVPDDVAWAGVDEYRMQVLMRLPLGCNWKVAIEAFSETYHVQG
                     IHREMLASTDDVNSPQRLWNRHGKLGQPYGIPSPRLRGGATDAEIWRSFIQTQGARMG
                     VTDPDTPAPGVPDGSTMRDVIEQAVRDEAATKGLDLSRFDAGQVLDLHQYNLFPNATV
                     IFLSDALSVMTATPGPTPDECVVTFFVAFRVPSADDPTPRPAVMDLEPGTVSLGLIFD
                     QDLENLQRVQKGLHQPGFTHLRLSAEECRIINLHRHLEAALGLEPGAEL"
BASE COUNT          391 a          938 c         1034 g          384 t
ORIGIN      
        1 ccgttcggcc aatggttgcc cgacgtgggc cttgtcccgc acgtgggaca gacatagagc
       61 ctgttcgcaa cgtcccgcac cacgaccagc gttaccacac gtcccgggaa agtgcgaaga
      121 aatgtttcgc cctggatcgg gtgcgcgaac cgtcggattg aggggacagc gggctggcgt
      181 cagacggcct ggagctcctc gacggcggcg gcgaagggct cgtcatcggc gggatcaatc
      241 ggggcgtgga gcaggacacc gtcggccagg tcgccgtagc ggtcacccag gacccgggcc
      301 agctcgtcgt agcggccggc ggcgaccacc gtgtcgagga cgtcgtcggt gaccgtggcc
      361 tccaggtcct cccaacgacc cgctttggtc atcgagcgga gccgatcgcc gaggtcctcc
      421 cagccgtgca gctcgagcgt ccggcggtag gcgggcgtgg agaagaggaa gccgagcatg
      481 tagcggctgc gggcgcgggc ctcggcgacg gcggcgtcgt cccggccggt cgcgaccatc
      541 cccccgacca cgacctgtac gtccgaccgg tcccgaccgg cctcggtcag gccctgctcc
      601 agcgccggga ggcagagctc gctgaggtag cgccggtcgg agttggtggg atgggtcacg
      661 tacccgtccc cgacggcgcc ggccagccgg caggcccggc ggttgacccc gcccagccag
      721 atggggaccg ggccgccgtc cagcgggccg gggttgaact cgggctggag tcgcgtcagc
      781 cggtagtgcg ggccctcgtg gcgcagcggc ccaccctcct ggaacgcggt gaagacggcg
      841 cggaccgcgg cgacgtagtc cgccatgcga tcgaccgggt cggcccactc gacgccgaac
      901 cgacccacga tgttgggctt gatctgggtg ccgaggccga gctggaagcg accgggcgcg
      961 aacccggcca gggaccaggt ggcgtaggcc acctgcatcg ggctgcggac gaaggccagg
     1021 gccagcgccg gccggacgac gagggtcgac gtgtgctcca gggccagggc acaggtcagg
     1081 aaggcgtcgt tggtggtctc ggccacgtgc agggcgtcga acccgagggc ctccacccga
     1141 cgggcatggg ctccgatgtc ggccagaggg gcgcggggat ccatccccgc tatcactctc
     1201 atttctgcgc ctcgcggcgc ggcccctgac gggggtacac atcccccgcg ccacctcgcc
     1261 gtcggcttgc gcagatcagc gacccaacgg cggtcactgt ggccaatgcg tcacagttcg
     1321 gcgccgggct cgaggccgag ggcggcctcc aggtggcggt ggaggttgat gatccggcac
     1381 tcctcggccg agaggcggag gtgggtgaag ccgggctggt gcaggccctt ctggacccgc
     1441 tggaggttct cgaggtcctg gtcgaagatc aggccgaggc tgacggtccc gggctcgagg
     1501 tccatcaccg cgggccgggg cgtggggtcg tccgccgacg ggacccggaa ggcgacgaag
     1561 aacgtgacca cgcactcgtc gggggtcgga ccgggcgtcg ccgtcatcac gctgagggcg
     1621 tcgctcagga agatgaccgt ggcgttgggg aagaggttgt actggtgcag gtccagcacc
     1681 tggccggcgt cgaaccggct caggtccagg cccttggtcg cggcctcgtc ccgcaccgcc
     1741 tgctcgatga cgtcccgcat cgtcgacccg tcgggcacgc ccggcgcggg ggtgtcgggg
     1801 tcggtgaccc ccatgcgggc gccctgggtc tggatgaagc tgcgccagat ctcggcgtcg
     1861 gtggccccgc cccgcagtcg cggcgacggg atgccgtagg gctggccgag cttgccgtgg
     1921 cggttccaga ggcgctgggg cgagttcacg tcgtcggtcg acgccaacat ctcgcggtgg
     1981 atcccctgta cgtggtacgt ctcgctgaac gcctcgatgg cgaccttcca gttgcacccg
     2041 agcggcaggc gcatgaggac ctgcatgcgg tactcgtcga cacccgccca ggccacgtcg
     2101 tcgggcacgc cctccagcca gtccgccagg ggctcggctt cgcgatcgag gttgacgaac
     2161 accagctcgc cccaggtgtc gacccggacc gggagcaggg gcaggtcgtc gttgcgcagc
     2221 ggaccgaagc ccttgcggga cgggatctcc cgcagctcac cccggaggtt ccacgcccaa
     2281 cggtggtagc cgcaacggat ctcggtgcgc ccttcgccct cgccctggca cagctcgctg
     2341 ccccggtgca gacagacgtt ctgaaaggcc cggagctcgc cgtcgtcgcc ccggacgatc
     2401 agcaccgaga gggatccgca ggtgaactcg caccagtcgc cgggctcggc cacctggccg
     2461 cgggcggcgg cgatgagcca ggtccggggc cagacccgct cggcctccga cgcggcccac
     2521 gccgaggagg tgtagcggga ggctgggatg cgggccggac cgaggggccc ggtctcgatg
     2581 gtggagccga ccggggcgtc gtcggtgagg tcggggcgat cctcgacgtc cgtcacctca
     2641 ggccgccggg cgggtgagct cccaggtgtt gtcccgcagg acacggcgct ggtcctcggc
     2701 ggacagctcc ttcacctcgg gcacgtagtc cagggggcgg ggcatgc
//
LOCUS       DASTFV010000003         3510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_10565, whole genome shotgun sequence.
ACCESSION   DASTFV010000003 DASTFV010000000
VERSION     DASTFV010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10565"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VFP02_00035"
     CDS             complement(<1..846)
                     /locus_tag="VFP02_00035"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9541437.1"
                     /translation="MTDKALVVSADGHILEPIDLFKTRLPKHLRDRAVWEEDFEIEPL
                     VEGGATVFRKLHTPGFDGWTVSRYRQTQGRTPEGDPEQIIEDLALDGVDVGVMHPNLS
                     LFGLYSDDHELSMAHARVYNDYLIERFTPYFDRLVPTAPVPLTDIGEAVAEIERVAAA
                     GFRAILLPAIAPMPYWSRDLDPVWAAAQAGGVQVFIHTQTGGVKVDDPESTTLKVVME
                     SAAQVNQPVTEKSASKRMVTQSIYSTIVPQQVLCELIGGGVPERFPDLHFALIEFNAH
                     WLGSLV"
     gene            909..1967
                     /locus_tag="VFP02_00040"
     CDS             909..1967
                     /locus_tag="VFP02_00040"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET9541438.1"
                     /translation="MTQRWQRRPDGSTWGDWGDDDELGRINLITPEKVLQGVREVEAG
                     ISFCLSLPLDFPGGTALNQRRPPPRVAPTEDMEGVADTFYNVHMSEMPDFGDPKYVDV
                     WADDVVTLALQYSTQWDSLAHVGAEFDADGDGIEEGVYYNGYRAGVDLVGPSDDARGD
                     GSGHASFARHLGLEHMAFHGVQGRGVLVDLAHHLGHDWQGVDLATLQEVMAADDVVVE
                     PGDMLLLHTGFATKVLEWNKEPDPRELFTTCSYVDAHDPALLDWIAESQISALVADNY
                     AVEGLLGKDKDPSRHSFLPIHHLCLFKLGVPLGELWYLHELATWLREHGRSRFLLTAP
                     PLRLPGIVGSPLTPVATV"
     gene            complement(1900..>3510)
                     /locus_tag="VFP02_00045"
     CDS             complement(1900..>3510)
                     /locus_tag="VFP02_00045"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9541439.1"
                     /translation="AGITGIYQLHRALEAGCSATLLEAGAGVGGTWYWNRYPGARFDS
                     ESYTYAYLFSQELFDDWEWQEHFAEQPETERYLNHVVDRFDLRRHIRFGAQVTSAVWD
                     DPSGTWVVTVVDGTEMRARFLVAATGVLSVPYFPDVPGREDFAGESVHTGLWPTEGVD
                     VRGQRVAVIGTGSSGVQVIPAILEDVASLTVYQRTANWCTPLNNAPITAEQQAQLRAD
                     FEALREVLNTSIHGFHHLPHDRGAFDDPDDERRAFFEKMWASPGFTKLTSNYLDLLTD
                     PSANAAWCDFLAEKVRGIVEDPDVAEQLIPHDHRFGEKRPPFVTGYFEAFNDPKVTLV
                     DLKATPILRLTPAGIETADGLQELDVVIWATGFDFGTGALARMGIRGRDGLTLEDHWA
                     DGPATFLGIQTTRFPNFFFPGGPHAGAGNNPRYNGDQVDFVIETLVHLRGRGDDVIEV
                     GAEGERLWTDMVDRGASDATSFGESSYYFGTNIPGKPRKYLLNSGGRLKLFKEFARVR
                     QNDWAAFRQSRRASTGSPRSPGGAAAAR"
BASE COUNT          531 a         1191 c         1261 g          527 t
ORIGIN      
        1 gaccagcgac cccagccagt gggcgttgaa ctcgatcagg gcgaagtgga ggtcagggaa
       61 gcgctcgggg acgccgccac cgatgagctc gcagaggacc tgctggggga cgatcgtgct
      121 gtagatcgac tgggtgacca tccgcttgga ggccgacttc tcggtcacgg gctggttgac
      181 ctgcgcggcg gactccatga cgaccttgag cgtcgtggac tccgggtcgt cgaccttcac
      241 cccgccggtc tgggtgtgga tgaacacctg cacgccgccg gcctgggcgg ccgcccacac
      301 cgggtcgagg tcccgtgacc agtagggcat cggggcgatg gcgggcagga ggatcgcccg
      361 gaagcccgcg gcggcgaccc gctcgatctc ggccacggcc tccccgatgt cggtgagcgg
      421 cacgggcgcg gtgggcacga ggcggtcgaa gtagggcgtg aaccgctcga tgaggtagtc
      481 gttgtagacc cgggcgtggg ccatcgacag ctcgtggtcg tcggagtaga gcccgaacag
      541 cgacaggttc gggtgcatca ccccgacgtc gaccccgtcg agggccaggt cttcgatgat
      601 ctgctcgggg tcgccctcgg gcgtgcgccc ctgggtctgg cggtagcggg agaccgtcca
      661 gccgtcgaac cccggcgtgt ggagcttgcg gaagaccgtg gccccgccct cgaccagcgg
      721 ctcgatctcg aagtcctcct cccagaccgc gcggtcccgc aggtgcttcg ggagacgggt
      781 cttgaagagg tcgatcggct cgaggatgtg cccgtccgcg gacacgacga gggccttgtc
      841 ggtcactgcc cgaaatctaa accaaccggt cggtacaagc aatgtgcgga cccctatggt
      901 ggacggccgt gacccagcgc tggcagcgac gccccgacgg ctcgacgtgg ggggactggg
      961 gggacgacga cgagctcggc cgcatcaacc tcatcacccc cgagaaggtc ctccagggag
     1021 tgcgggaggt cgaggccggg atcagcttct gcctgagcct gcccctcgac ttcccgggcg
     1081 ggacggccct gaaccaacgg cgaccgccgc cccgcgtggc gcccaccgag gacatggagg
     1141 gcgtcgccga caccttctac aacgtccaca tgagcgagat gccggacttc ggcgacccca
     1201 agtacgtcga cgtgtgggcc gacgacgtag tcaccctcgc cctgcagtac tcgacccagt
     1261 gggactccct cgcccatgtc ggggccgagt tcgacgccga cggcgacggc atcgaggaag
     1321 gcgtctacta caacggctac cgggccgggg tcgacctcgt cggcccgagc gacgacgccc
     1381 ggggcgacgg cagcggacac gcctccttcg cccgccacct cggcctggag cacatggcgt
     1441 tccacggggt gcagggccgc ggcgtcctcg tcgacctggc ccaccacctg ggccacgact
     1501 ggcagggggt cgacctggcc acgctccagg aggtcatggc ggccgacgac gtggtggtgg
     1561 agcccggcga catgctcctg ctccacaccg gcttcgcgac caaggtcctc gagtggaaca
     1621 aggagccgga cccgagggag ctcttcacca cgtgctccta cgtggacgcc cacgacccgg
     1681 ccctgctcga ctggatcgcc gagtcccaga tctcggccct cgtggccgac aactacgccg
     1741 tcgagggcct cctcggcaag gacaaggacc cgagccggca ctcgttcctg ccgatccacc
     1801 acctgtgcct gttcaagctc ggcgtcccgc tcggcgagct gtggtacctg cacgagctgg
     1861 cgacctggct gcgggagcac ggccgcagcc ggttcctgct caccgcgccg ccgctgcgcc
     1921 tcccggggat cgtgggctcc ccgttgacgc ccgtcgcgac tgtctgaacg cggcccagtc
     1981 gttctggcgg acgcgggcga actccttgaa gagcttgagg cgcccacccg agttcagcag
     2041 gtacttccgc ggcttccccg ggatgttggt cccgaagtag tagctgctct cgccgaagga
     2101 ggtggcgtcc gaggcgcccc ggtcgaccat gtcggtccat agccgctcgc cctcagctcc
     2161 cacctcgatc acgtcatcgc cgcgcccgcg taagtggacg agcgtctcga tgacgaagtc
     2221 cacctggtcg ccgttgtacc gggggttgtt gccggccccg gcgtggggcc cgccggggaa
     2281 gaagaagttc gggaagcggg tggtctggat cccgaggaag gtcgcaggac cgtcggccca
     2341 gtggtcctcg agggtcaggc cgtcccggcc gcggatgccc atgcgggcca gcgccccggt
     2401 accgaagtcg aagccggtgg cccagatgac gacgtcgagc tcctgcaggc cgtcggccgt
     2461 ctcgatgccc gccggggtca ggcgcaggat gggcgtcgcc ttcaggtcga cgagcgtgac
     2521 cttcggatcg ttgaacgcct cgaagtagcc ggtgacgaac ggggggcgct tctcgccgaa
     2581 ccggtggtcg tgggggatca gctgctcggc gacgtcgggg tcctcgacga tgccacgcac
     2641 cttctcggcc aggaagtcgc accacgccgc gttcgccgac gggtcggtca gcaggtcgag
     2701 gtagttgctg gtgagcttcg tgaagcccgg gctggcccac atcttctcga agaacgcccg
     2761 gcgctcgtcg tcggggtcgt cgaacgcgcc ccggtcgtgg gggaggtggt ggaacccgtg
     2821 gatcgaggtg ttcagcacct cgcgcagcgc ctcgaagtca gcccgtagct gcgcctgctg
     2881 ctcggccgtg atcggggcgt tgttgagggg ggtgcaccag ttggcggtgc gctggtagac
     2941 ggtcagggag gcgacgtctt cgaggatggc ggggatcacc tgcacgccgc tggacccggt
     3001 gccgatgacg gcgacccgct ggccccggac gtccacgccc tccgtcggcc agaggccggt
     3061 gtggaccgac tcgccggcga agtcctcccg ccccggcacg tcagggaagt acggcaccga
     3121 gaggacaccg gtggccgcga cgaggaaccg ggcccgcatc tccgtgccgt cgacgacggt
     3181 cacgacccag gtccccgacg ggtcgtccca gaccgcggag gtgacctggg cgccgaagcg
     3241 gatgtggcgg cggaggtcga accggtcgac gacgtggttc aggtagcgct cggtctcggg
     3301 ctgctcggcg aagtgctcct gccactccca gtcgtcgaac agctcctggg agaagaggta
     3361 ggcgtaggtg tagctctccg agtcgaaccg ggccccgggg tatcggttcc agtaccaggt
     3421 gccaccgacg ccggcgccgg cctccagcag ggtcgccgag cacccggcct cgagggcccg
     3481 gtgcagctgg tagatgccgg tgatgccggc
//
LOCUS       DASTFV010000004         3136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_12991, whole genome shotgun sequence.
ACCESSION   DASTFV010000004 DASTFV010000000
VERSION     DASTFV010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12991"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..227)
                     /locus_tag="VFP02_00050"
     CDS             complement(75..227)
                     /locus_tag="VFP02_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541440.1"
                     /translation="MELLRTPDEAFADLPGYPFAPHYVEVPTGDGDQLVWTYGPMFTA
                     RLERVG"
     gene            282..2567
                     /locus_tag="VFP02_00055"
     CDS             282..2567
                     /locus_tag="VFP02_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET9541441.1"
                     /translation="MSPSGEPQFAGRIGRLVEDSEPWWPDAPRPPAFPTGTRAPNVVV
                     VLLDDTGFAHFGCYGSTIETPTIDRLAAGGLRYTNFHTTALCSPTRACLLTGRNHHTV
                     GMRGISNWDPGFPHMRGAITPHAATLGEVLGDHGYATFALGKWHLAPMIECSAAGPHD
                     NWPLQKGFDRFYGFLNGETDQFHPELTYDNHPIDPPGDPATGYHVSEDLVDHAIEFVS
                     DLRSARPDRPFFTYLAFGATHAPHQAPPEYLAKYRGRFDGGWDVARAEWFGRQQAMGI
                     VPEGTELAPRNFGVRAWDDLSDNQRLFACRLQEAFAAFLDHTDAQIGRFVDYLEASGE
                     LDNTVFVVFSDNGASQEGGAEGVMNEFQWFNGFPEDVDHIVADRLDDIGGPHSHTNYP
                     WGWAQAGNTPLKWYKQNTFGGGVRDPLVVHWPDGIADRGGVRHQFCHVVDLAPTFFEV
                     LGIEPPDERRGVDQLPVAGTSLAYTWAEPEAPTRKTVQYFEQMGHRGIWQDGWKAVTY
                     HWKGTPFADDTWELYHLDEDFSESRNLAETHPEKLAELIDLWWAEAEAHGVPPLDDRT
                     IELFASEPRPGTPHATTTYVYRPPVAHLPSDVSPRLGGRPYTITAYIERASADEGGVI
                     VAMGSHAAGYSFFVKDGRLGFDYNDFGHHTVLTADRDVPLGRLPVAVRFTREQAGAGT
                     FTLLVDDVTVASSPIPRRVRIFGSMGLDVGRDGLSPVSEQYEGAFPFTGTLHEVRYDI
                     ISRRSEAADAAVEERAGFGTQ"
     gene            complement(2598..>3136)
                     /locus_tag="VFP02_00060"
     CDS             complement(2598..>3136)
                     /locus_tag="VFP02_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9541442.1"
                     /translation="VDAMLDLLGEGHLTPGAAAVAERSGVSLRSVFRYFDDMDSLTER
                     AIARQMERAAPLFDLLDDHGPLDVRVGRLASHRAVQYNRLAPFARVAIVRSAQYPAIA
                     VGLATRRDALRRQVEALFGRELDPLDRPVRDEVLSALEAATSFEVVDLLVAHRGLSTP
                     AVEKILRRTVLGILSTAA"
BASE COUNT          482 a         1168 c         1045 g          441 t
ORIGIN      
        1 gtccggccac gacggcgtcg agtccttgta cgtgcgaccg atgcggccgc cgaagtccgg
       61 ttcgttgggt gcggtcatcc gacgcgctcg agccgcgccg tgaacatcgg gccgtacgtc
      121 cagacgagct ggtcgccgtc gcccgtcggt acctcgacgt agtggggggc gaacggatag
      181 cccgggaggt cggcgaaggc ctcgtcgggg gtgcgcagca gctccattgc ggttgacgct
      241 aactatttac actaggagtg tcaatagtta gcgtcacggc catgagcccg tcgggggagc
      301 cgcagttcgc cggtcgcatc ggtcgcctgg tcgaggactc cgagccgtgg tggccggacg
      361 ccccccgacc gccggccttc ccgaccggga cccgggcgcc caacgtggtg gtggtcctcc
      421 tggacgacac cggcttcgcc cacttcggct gctacggctc gaccatcgag acgcccacca
      481 tcgaccgtct cgccgccggg gggcttcgct acacgaactt ccacaccacc gccctctgct
      541 cgccgactcg ggcctgcctg ctgaccggcc gcaaccacca caccgtcggc atgcgcggca
      601 tctccaactg ggaccccggc ttcccgcaca tgcggggcgc gatcacgccc catgccgcca
      661 ccctgggcga ggtcctgggc gaccacggct acgccacgtt cgccctcggc aagtggcacc
      721 tggcccccat gatcgagtgt tcggccgccg gcccccacga caactggccg ctgcagaagg
      781 gcttcgaccg cttctacggc ttcctcaacg gcgagaccga ccagttccac cccgagctga
      841 cctacgacaa ccaccccatc gacccgcccg gtgaccccgc caccgggtac cacgtgtccg
      901 aggacctcgt cgatcacgcc atcgagttcg tgtccgacct gcggagcgca cgaccggacc
      961 gcccgttctt cacctacctg gccttcgggg ccacgcacgc cccccaccag gccccacccg
     1021 agtacctggc caagtaccgg ggtcgcttcg acgggggctg ggacgtcgcc cgggccgagt
     1081 ggttcgggcg ccagcaggcc atgggcatcg tgcccgaggg cacggaactg gccccgcgca
     1141 acttcggggt ccgggcctgg gacgacctgt cggacaacca gcgcctcttc gcctgtcgcc
     1201 tgcaggaggc gttcgccgcg ttcctcgacc acaccgacgc ccagatcggg cgcttcgtcg
     1261 actacctcga ggccagcggt gagctggaca acaccgtctt cgtcgtcttc tccgacaacg
     1321 gggccagcca ggagggcggg gccgaagggg tcatgaacga gttccagtgg ttcaacggct
     1381 tcccggagga cgtggaccac atcgtggccg atcggctcga cgacatcggc ggaccccaca
     1441 gccacaccaa ctacccgtgg ggctgggccc aggccggcaa cacccccctc aagtggtaca
     1501 agcagaacac cttcggcggc ggcgtgcggg accccctggt ggtgcactgg cccgacggca
     1561 tcgccgaccg gggcggtgtc cgtcaccagt tctgccacgt ggtcgacctc gcccccacct
     1621 tcttcgaggt cctcggcatc gagccgcccg acgagcggcg gggcgtcgac cagctcccgg
     1681 tggccggcac ctcactggcc tacacctggg ccgagcccga ggcccccacc cgcaagacgg
     1741 tccagtactt cgagcagatg ggccaccgcg gcatctggca ggacgggtgg aaagccgtga
     1801 cgtaccactg gaagggcacc cccttcgccg acgacacgtg ggagctgtac cacctcgacg
     1861 aggacttctc ggagtcccgg aacctggccg agacccaccc cgagaagctg gccgagctca
     1921 tcgacctctg gtgggccgag gccgaggccc acggcgtccc gcccctggac gaccgcacga
     1981 tcgagctctt cgcctccgag ccccgtcccg gcacgcccca tgccaccacc acctacgtct
     2041 accgccctcc ggtcgcccat ctcccctcgg atgtctcgcc ccgcctgggc ggccggccgt
     2101 acacgatcac cgcatacatc gagcgggcgt cggcggacga gggcggggtg atcgtggcca
     2161 tgggcagcca cgccgcgggg tacagcttct tcgtgaagga cgggcgcctg ggcttcgact
     2221 acaacgactt cggccaccac accgtgctca ccgccgaccg ggacgttccc ctgggtcgac
     2281 tccccgtcgc cgtccgcttc acccgggagc aggcgggggc gggcacgttc accctgctcg
     2341 tcgacgatgt caccgtggcc tcgtcaccca tccctcgccg ggttcgcatc ttcggctcga
     2401 tgggcctcga tgtcggccgg gacgggctct cgccggtcag cgagcagtac gagggggcct
     2461 tcccgttcac cgggaccctg cacgaggtcc gctacgacat catctcgcga cggtccgaag
     2521 cggccgatgc cgcggtcgag gagcgggccg gcttcggcac gcagtgagga ccgaggtgtc
     2581 cggtacgacg ccggcgatca ggcggcggtg ctcaggatcc ccagcacggt ccgccgaaga
     2641 atcttctcga cggcaggcgt cgacaggccc cgatgggcga cgagcaggtc cacgacctcg
     2701 aaggaggtgg cggcctcgag ggccgacagg acttcgtccc ggacgggccg atcgagcggg
     2761 tccagctccc ggccgaagag ggcctccacc tggcggcgca gcgcgtcgcg ccgggtggcc
     2821 aggcccacgg cgatggccgg gtactgcgcg gaccgcacga tcgcgacccg ggcgaacgga
     2881 gcgaggcggt tgtactgcac agctcggtgg gatgcgaggc ggccgactcg gacgtcgagc
     2941 gggccgtggt cgtcgaggag gtcgaacagc ggcgccgccc gctccatctg acgggcgatg
     3001 gcccgctcgg tgagcgagtc catgtcgtcg aagtagcgga acaccgaccg gagcgagacc
     3061 ccggagcgct cggccacggc cgccgcaccc ggcgtgaggt gaccttcgcc caggaggtcg
     3121 agcatggcgt cgacca
//
LOCUS       DASTFV010000005         3595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_22550, whole genome shotgun sequence.
ACCESSION   DASTFV010000005 DASTFV010000000
VERSION     DASTFV010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22550"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFP02_00065"
     CDS             complement(<1..180)
                     /locus_tag="VFP02_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET9541443.1"
                     /translation="MTTGIDAVDGRLLALLHDEPRLSVLELARRAGVARGTAQARLDR
                     LQARGVVRGFGPEVDP"
     gene            338..1474
                     /gene="hppD"
                     /locus_tag="VFP02_00070"
     CDS             338..1474
                     /gene="hppD"
                     /locus_tag="VFP02_00070"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HET9541444.1"
                     /translation="MSTATVAGPSSATAIAAPELLGFDHLEWWVGNARHAAHFWAAAF
                     GFEIVAYAGPETGVSDRQSYVVQQGELCFVLTSALDDCSEVAHHVAAHGDGVRDVAYR
                     VADVDESFDRLVTRGGVPVLPPTTFADDFGSARRATFTAYGRTVHSLVDRARHGGPFL
                     PGFEAVDLPIDAGPAVGLRAYDHVVANVEEGSLDRWVDWYRALFGFDVLQSFGEEAIS
                     TEYSALRSTVVWNHGAVVQPINEPAPGRRKSQIEEYLDYYGTAGVQHVAMSTDDIVAS
                     VAALRRRGVRFMRVPDNYYDEAPRRMDDLGADLPWARLAELGILVDRDHGGYLLQIFT
                     EPVASRPTCFMEIIQREGAQGFGEGNFKALFTSIEAEQARRGNL"
     gene            1479..2303
                     /locus_tag="VFP02_00075"
     CDS             1479..2303
                     /locus_tag="VFP02_00075"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:NF012571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HET9541445.1"
                     /translation="MITAPAPFALPAEHPGFGDPGYRERRARIAEIGARYEPGSPIPD
                     VHYTAEEDEVWRNVSAELGEKHRRLACREIVDAAHRLDLPTDRVPQLREVDQCLQELS
                     GFRIRPVPGLVPTREFYGALAERTFLATQYIRHHSVPFYTPEPDVLHEVIGHATMLAD
                     PALADLYEAAGQASLRCRTDEDLEFFSRVFWFTIEFGVLWQDGDLLAYGAGLLSSYAE
                     IEVFRSAEVRPWDLPTMGRLDYDITQYQPVLFAAASFDQMTEDLGAFLATYPGSAP"
     gene            complement(2401..3414)
                     /gene="glnII"
                     /locus_tag="VFP02_00080"
     CDS             complement(2401..3414)
                     /gene="glnII"
                     /locus_tag="VFP02_00080"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase GlnII"
                     /protein_id="HET9541446.1"
                     /translation="MTYRAEYIWIDGSEPTAEIRSKTKIVPDGGELGIWGFDGSSTNQ
                     ASGDNSDVVLQPVFTCPDPIRGGDNVLVMCETQLTADFSPHPTNTRALARAAFEKYGD
                     QEPLFGLEQEYTMLDPASSWPYGFPQHGYPGPQGPYYCGVGAVKIAGRDIVEEHTTLC
                     IEAGLAISGTNAEVMPGQWEFQIGPVDTVAVGDHLWLARYLLYRIGEKYNVEISIDAK
                     PMKGDWNGAGMHTNFSTNKMREDYEAIITACEALGSAGKPDEHIDGYGQGIQDRLTGA
                     HETQRFDQFSYGVSDRGASIRIPWQVAKEGKGYIEDRRPNANADPYVVAALVTNTVCE
                     ALV"
BASE COUNT          524 a         1297 c         1213 g          561 t
ORIGIN      
        1 cgggtcgacc tcggggccga agccgcggac gacgccccgg gcctggaggc ggtcgaggcg
       61 ggcctgggcc gtgccccggg ccacgcccgc ccggcgggcc agctcgagga cgctcaggcg
      121 gggctcgtcg tggaggaggg cgagcagccg cccgtcgacg gcatcgatcc cggtcgtcac
      181 cagccctcct cccgggctgg gcaaggtgcc caaccgatcg acaggctagt acgttgagag
      241 tgcacaactt gtccagtcag acagccatct gttgacgaca tagcccggga aggcgtccaa
      301 tagggcgaca cactggtcaa cacgagagga atggggaatg agcaccgcca ccgtcgccgg
      361 tccgtcatcc gccaccgcaa tcgccgcacc ggagctcttg ggcttcgatc acctcgagtg
      421 gtgggtgggc aatgcccgcc acgccgccca cttctgggcc gccgccttcg gcttcgagat
      481 cgtggcctac gccgggcccg agacgggcgt gtccgaccgt cagtcctacg tcgtccagca
      541 gggtgaactc tgcttcgtcc tcacctccgc cctcgacgac tgctccgagg tggcccacca
      601 cgtggccgct cacggcgacg gcgtccgcga cgtcgcctac cgcgtggccg acgtcgacga
      661 gtccttcgat cgcctggtca cccgaggcgg cgtgcccgtg ctccccccga ccacgttcgc
      721 ggacgacttc ggctcggccc ggcgggccac gttcacggcc tacggccgga cggtgcactc
      781 cctggtcgac cgggcccgcc acggcggccc gttcctgcct ggcttcgagg ccgtcgacct
      841 cccgatcgac gccggcccgg ccgtcggcct gcgggcctac gaccacgtcg tggccaacgt
      901 ggaggaggga agcctcgacc gctgggtcga ctggtaccgg gccctgttcg gcttcgacgt
      961 cctccagtct ttcggcgagg aggccatctc caccgagtac tccgctctgc gttccaccgt
     1021 ggtgtggaac cacggcgccg tcgtccagcc catcaacgag cccgccccgg gtcggcgcaa
     1081 gagccagatc gaggagtacc tcgactacta cggcaccgcc ggcgtgcagc acgtggccat
     1141 gagcaccgac gacatcgtgg ccagcgtggc cgcactgcgc cggcgcggcg tgcgcttcat
     1201 gcgggtgccc gacaactact acgacgaggc ccctcgacgc atggacgacc tgggcgccga
     1261 cctcccctgg gcccgcctcg cggagctcgg catcctggtc gaccgcgacc acggcgggta
     1321 tctgctccag atcttcaccg agcccgtggc cagccggccg acctgcttca tggagatcat
     1381 ccagcgggaa ggggcacagg gcttcggcga gggcaacttc aaggccctgt tcacctcgat
     1441 cgaggccgag caggcccggc gcgggaacct ctgagcccat gatcaccgcg cccgcgccct
     1501 tcgccctccc ggctgagcac cccggcttcg gcgatccggg ctaccgggag cggcgggcgc
     1561 gcatcgccga gatcggggcc cggtacgagc cggggagccc gatccccgac gtgcactaca
     1621 ccgccgagga ggacgaggtc tggcgcaacg tctccgccga gttgggcgag aagcaccgtc
     1681 gcctcgcctg ccgggagatc gtcgacgccg cccaccgcct cgatctgccc accgatcggg
     1741 tgccccagct ccgcgaggtc gaccagtgcc tgcaggagct cagcgggttc cgcatacgac
     1801 cggtgccggg cctcgtcccg acccgggagt tctacggggc cttggccgag cgcacgttcc
     1861 tggccacgca gtacatccgc caccactcgg tccccttcta cacccccgag cccgacgtcc
     1921 tccacgaagt catcggccac gccacgatgc tggccgaccc cgcgctggcc gacctgtacg
     1981 aggccgccgg ccaggcctcc ctgcgctgtc gcaccgacga ggacctcgag ttcttcagcc
     2041 gggtcttctg gttcacgatc gagttcggcg tcctgtggca ggacggcgac ctcctcgcct
     2101 acggggccgg cctcctgtcc tcctacgcgg agatcgaggt gttccggtcg gccgaggtgc
     2161 gcccctggga cctgccgacc atgggccgcc tcgactacga catcacccag taccagccgg
     2221 tgctcttcgc cgcggcgtcc ttcgaccaga tgaccgagga cctcggcgcc ttcctggcga
     2281 cctaccccgg cagcgccccc tgacctcgag ttcagggggc gggacgcgga aacagtccgg
     2341 ggccgcctcc gcccggccgg ccccggactg tcacgctctg gggggaactg caggcggggc
     2401 ctagacgagg gcctcgcaga ccgtgttggt caccagggcg gcgacgacat aggggtcggc
     2461 gttggcgttg gggcgccggt cctcgatgta gcccttgccc tccttggcca cctgccacgg
     2521 gatgcggatc gaggcccctc gatccgacac gccgtagctg aactggtcga agcgctgggt
     2581 ctcgtgagca ccggtgaggc ggtcctggat gccctggccg tacccgtcga tgtgctcgtc
     2641 gggcttcccg gccgaaccca gtgcctcgca ggcggtgatg atggcctcgt agtcctcgcg
     2701 catcttgttg gtcgagaagt tggtgtgcat cccggcgccg ttccagtcac ccttcatggg
     2761 cttggcgtcg atggagatct cgacgttgta cttctcaccg atgcggtaga ggaggtagcg
     2821 ggcgagccac aggtggtcgc ccacggccac ggtgtcgacc gggccgatct ggaactccca
     2881 ctggccgggc atgacctcgg cgttggtgcc ggagatggcc agaccggcct cgatgcacag
     2941 ggtggtgtgc tcctcgacga tgtcccgccc ggcgatcttc accgcgccga cgccgcagta
     3001 gtacgggccc tgggggccgg ggtagccatg ctgcgggaac ccgtagggcc agctcgaggc
     3061 cgggtccagc atcgtgtact cctgctcgag gccgaacagg ggctcctggt cgccgtactt
     3121 ctcgaaggcg gcccgggcca gcgcccgcgt gttggtcggg tgcggcgaga agtccgccgt
     3181 gagctgggtc tcgcacatga ccaggacgtt gtccccgccc cgaatggggt ccgggcaggt
     3241 gaagacgggc tggaggacga cgtcggagtt gtcaccgctc gcctggttcg tgctggagcc
     3301 gtcgaagccc cagatgccca gctcaccgcc gtcggggacg atcttggtct tggaacggat
     3361 ctcggccgtc ggctccgaac cgtcgatcca gatgtactca gccctgtagg tcacgctcac
     3421 ctcttgtctc ggggggccgt tcgggaacgc ccatcggcca cgggtgggga caggatcacc
     3481 gccacccatt tcggagcggt tgcacaaatg tgaacgccat gtgaacgacc gcggcaaggt
     3541 cgtgacaccg cgccgggaac cgtcatcatg acccgatgga gcgtcagcag gagta
//
LOCUS       DASTFV010000006         2725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_23125, whole genome shotgun sequence.
ACCESSION   DASTFV010000006 DASTFV010000000
VERSION     DASTFV010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23125"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1800)
                     /gene="valS"
                     /locus_tag="VFP02_00085"
     CDS             complement(<1..1800)
                     /gene="valS"
                     /locus_tag="VFP02_00085"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET9541447.1"
                     /translation="MSPERSVPDKPSLEGLEARWGEEWEREGTYRFDRTKTRDQIYSV
                     DTPPPTVSGSLHVGHVFSFTHTDTIVRYQRMRGRECFYPMGWDDNGVPTERRVENFYG
                     VRCDPSLPYDPDFRPPAEPPKGNRRDDLVRCSRRNFIDLCFGLSAEDEQAFEELWRHL
                     GLSVDWSLTYTTINDQSRRASQRAFLRNLARGEAYQSEAPTLWDVRFEMAVSQAELED
                     REQPGAYHQVAFGRDGGDPVVIDTTRPELIPACVALVAHPDDERYQPFFETQVRTPLF
                     GVPVPVKAHRLADPEKGTGIAMICTFGDTTDVTWWRELDLPVRAVVGRNGRLLPDAPH
                     EFTDEATDLYESELAGKTLFSAKERIVELLGEAGALRGEPRKITHPVKFYEKGDKPLE
                     IVTSRQWYIRNGGREDDLRQALIARGKELDWHPGYMRARYEDWIEGLNGDWLISRQRY
                     FGVPIPVWYGVGADGEVDHDTVLVPDEDALPIDPTSEAPPGFDEGQRGQPGGFVGEAD
                     IMDTWATSSLSPQVVGGWVDDEDLFQRVFPMDLRPQGHDIIRTWLFSTVVRSHFEHDT
                     APWRHAAISGWILDPDRKKMSKSKGNVVTPMAML"
     gene            1887..>2725
                     /gene="cysS"
                     /locus_tag="VFP02_00090"
     CDS             1887..>2725
                     /gene="cysS"
                     /locus_tag="VFP02_00090"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET9541448.1"
                     /translation="MLSLFDTAAGRARPVEFREPGQATMYVCGPTVYGPPHLGHGRFS
                     LVFDILRRYLEWTGLEVTYVSNITDIDDQIINKGEAEGRDPAEVAVEFEAKWYEGMDL
                     IGVKRPTFDPHATAYVDQMVQLIADLIAADRAYETSDGVYLATEGIEGYGTLARQSLD
                     SLRSGARIEVNDEKRSPLDFVLWKKAKPGEPTWDSPWGPGRPGWHTECVVMSLELLGE
                     GFDIHGGGQDLAFPHHENEWAQAKALDKAFARHWVHNGFVEVAGEKMSKSLGNYTDLL
                     DLAT"
BASE COUNT          406 a          910 c          984 g          425 t
ORIGIN      
        1 gagcatggcc atcggcgtca ccacgttgcc cttggacttc gacatcttct tgcggtccgg
       61 gtcgaggatc cagccggaga tggcggcgtg ccgccaggga gcggtgtcgt gctcgaagtg
      121 ggaccgcacc acggtggaga acagccaggt gcggatgatg tcgtggccct gggggcgcag
      181 gtccatcggg aagacccgct ggaacaggtc ctcgtcgtcg acccacccgc ccacgacctg
      241 cggggagagc gaggacgtgg cccaggtgtc catgatgtcg gcctccccca cgaagccgcc
      301 cggttggccg cgctggccct cgtcgaagcc gggaggcgcc tcggaggtcg gatcgatggg
      361 gagggcgtcc tcgtcgggca cgagcacggt gtcgtggtcg acctcgccgt cggccccgac
      421 gccgtaccag accgggatgg gcacgccgaa gtagcgctgg cggctgatca gccagtcgcc
      481 gttgaggccc tcgatccagt cctcgtacct cgcccgcatg tagccggggt gccagtcgag
      541 ctccttgccc cgggcgatga gggcctgccg caggtcgtcc tcccgcccgc cgttgcggat
      601 gtaccactga cgggaggtga cgatctcgag gggcttgtcg cccttctcgt agaacttgac
      661 cgggtgggtg atcttgcggg gctcgccccg cagcgctccg gcctcgccca gcagctcgac
      721 gatgcgctcc ttggcgctga acagggtctt gccggcgagc tcggactcgt agaggtcggt
      781 ggcctcgtcg gtgaactcgt ggggcgcgtc ggggagcagc cggccgttcc gacccacgac
      841 ggcccgcacg ggcaggtcca gctcccgcca ccaggtcacg tcggtggtgt cgccgaaggt
      901 gcagatcatg gcgatgccgg tgcccttctc cgggtcggcc aggcggtggg ccttgaccgg
      961 gaccgggacg ccgaacaacg gagtccggac ctgggtctcg aagaacggct ggtagcgctc
     1021 gtcgtcgggg tgggcgacga gggcgacgca ggcggggatg agctcgggcc gggtcgtgtc
     1081 gatgaccacg gggtcgccgc cgtcccgtcc gaacgccacc tggtggtagg ccccgggctg
     1141 ctcgcggtcc tccagctcgg cctgggacac ggccatctcg aagcggacgt cccacagcgt
     1201 cggggcctcg gactggtagg cctcgccccg ggccaggttg cgcaggaacg cccgctggct
     1261 cgctcggcgg gactggtcgt tgatcgtggt gtaggtcagg gaccagtcca ccgacagccc
     1321 gagatgccgc cacagctcct cgaaggcctg ctcgtcctcg gcgctgaggc cgaagcagag
     1381 atcgatgaag ttgcgccgcg agcagcgcac gagatcgtcc cgacggttgc ccttcggggg
     1441 ctcggccggg ggccggaaat cggggtcgta gggcagcgag gggtcacagc gcaccccgta
     1501 gaagttctcc acccgacgct cggtgggcac gccgttgtcg tcccagccca tggggtagaa
     1561 gcactcgcgg ccccgcatgc gctggtagcg gacgatcgtg tcggtgtggg tgaacgagaa
     1621 cacgtggccc acgtgcaggg agccactgac ggtcggcggt ggcgtgtcga ccgagtagat
     1681 ctggtcgcgg gtcttggtcc ggtcgaagcg gtaggtcccc tcgcgctccc attcctcgcc
     1741 ccagcgcgct tcgaggcctt ccagcgaggg cttgtcggga acggaccgct ctgggctcat
     1801 gatccggccc aagctaccgg tggggtcact cggtccgaag acgaaacctc cccggcgtga
     1861 ggcagggacc tcggtaccgt ctcgctgtgc tctcgctgtt cgacaccgct gccggccgcg
     1921 cccgccccgt ggagttccgt gaaccgggcc aggcgacgat gtatgtctgt ggcccgaccg
     1981 tctacgggcc gccccacctg ggccacgggc gcttcagcct ggtgttcgac atcctccgcc
     2041 ggtacctcga gtggaccggg ctcgaggtca cctatgtctc caacatcacc gacatcgacg
     2101 accagatcat caacaagggg gaggctgagg gtcgcgaccc cgccgaggtg gccgtcgagt
     2161 tcgaggccaa gtggtacgag ggcatggacc tcatcggggt caagcgcccg accttcgacc
     2221 cccacgccac cgcctacgtg gaccagatgg tccagctcat cgccgacctg atcgccgccg
     2281 accgggcgta tgagaccagc gacggcgtct acctggcgac cgagggcatc gagggctacg
     2341 gcacgctggc ccgccagtcg ctggactcgc tgcgctccgg ggcccgcatc gaggtcaacg
     2401 acgagaagcg ctcgcccctg gacttcgtgc tttggaagaa ggccaagccg ggggagccga
     2461 cgtgggactc gccgtgggga ccgggccggc cgggctggca caccgagtgc gtggtcatgt
     2521 cgctcgagtt gctgggcgag ggcttcgaca tccacggcgg tggccaggac ctggcgttcc
     2581 cccaccacga gaacgagtgg gcgcaggcca aggccttgga caaggcgttc gcccggcact
     2641 gggtccacaa cgggttcgtg gaggtggcgg gggagaagat gtccaagtcc ctcggcaact
     2701 acaccgacct gctcgacctg gccac
//
LOCUS       DASTFV010000007         2701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_25067, whole genome shotgun sequence.
ACCESSION   DASTFV010000007 DASTFV010000000
VERSION     DASTFV010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25067"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VFP02_00095"
     CDS             complement(<1..840)
                     /locus_tag="VFP02_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9541449.1"
                     /translation="MSNEDLAPLILALLLLTVGANVLGQLFARMRQPKVVGEILAGVL
                     LGPSLLGHLAPGATASIFGSSTDDPSSMVLGFLYHFGLLLLMFVSGASVRHVLGKENR
                     RPTAWILGLGTPLPFLLALVIAPMLPLGQFTGEAGSESAVVLVFAVAAAVTSIPVITK
                     IFHDLGILHTRFASLILGAAVLEDIALWGVLAIATAIASATLSAEQGQIAGTVSVHVV
                     SNIVYVFVAMVLAPSVLRWASRARWNTLASQSPVAWIVAIMFGYVVVAAAFDVTLVFA
                     AFLA"
     gene            1400..>2701
                     /locus_tag="VFP02_00100"
     CDS             1400..>2701
                     /locus_tag="VFP02_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541450.1"
                     /translation="MSENPSDTTPHLIMGTAGEDVRAFAQVGNTMYAGGLFNQVQDWA
                     RTTTYARQNFVAFDRETGVISPLNLAFNGRVGGIEATADGTALFIAGAFSTVNDITRR
                     GLVKYDLVNNRIDPTFLPGNMRTVSDIKLANGSLIAAGNFTGHVRALNPTTGANLGTI
                     NITVAGVVNPDDETRVRHIAVSPNGTRLVATGNFATVNGQGRRRAFMLNLGPTATLST
                     WHAPRFDEDCYVFARPDSAQGVDFSPDGTYFVIVSTGGPTGTNGMCDAAARFETANVS
                     ANAAPTWINWTGGDSLYSVAITGPAVYVGGHQRWLDNPLGFDSAGPGAVSRPGIGAIN
                     PVTGRALAWNPTKSRNHGTMVLYPTPQGLWVGSDGEEFGHEDHAGIGFAPLGDDEDTT
                     DPTVGVVTPPGGAVYARNRVVNADFSCADEAGGSGLASCVGT"
BASE COUNT          492 a          913 c          912 g          384 t
ORIGIN      
        1 ggcgaggaag gcggcgaaga cgagggtcac atcgaaggct gcggcgacca ccacgtagcc
       61 gaacatgatg gccacgatcc aagccaccgg gctctgcgag gcgagggtgt tccagcgggc
      121 tcgactggcc cagcgcagga cggacggggc cagcaccatc gcaacgaaga cgtagacgat
      181 gttggagacc acgtgcacgg agacggtgcc ggcgatctgg ccctgctcgg cggagagggt
      241 cgccgacgcg atcgcggtgg cgatcgccag aaccccccac agcgcgatgt cctcgagtac
      301 ggccgcgccg aggatcagcg aggcgaagcg ggtgtggagg atcccgaggt cgtggaagat
      361 cttggtgatg accgggatcg aggtgacggc ggccgcgacg gcgaagacga ggacgaccgc
      421 ggactcgctc cctgcctcac cggtgaactg cccgaggggc aacatcggcg cgatgaccag
      481 agccaggagg aacggcagcg gggttccgag cccgaggatc cacgccgtcg gcctgcggtt
      541 ctccttcccg agcacatgac gaacgctcgc gcccgagacg aacatcagca gcagcaaccc
      601 gaagtggtac aggaacccga ggaccatcga ggaggggtcg tccgtcgacg agccgaagat
      661 cgacgcggtc gcgccgggtg ccaggtggcc caacagggac gggccgagca gcacgccggc
      721 caagatctcg cccaccacct tgggctgacg catgcgggcg aacaactgcc cgaggacatt
      781 cgcccccacc gtcagcagca gcagcgccaa gatcagcggc gccagatcct cgttcgacat
      841 ctctcaccct cccgcgggtg taagccccaa gacacgtcga cgaggaagag ttcggggatc
      901 caactcccgg tcaaccgccg ttgaccgccc gcctgcttcg acgaaggtag acgtatgatc
      961 acgagtcggt aaacccccaa tctgccgagc gttggcagac aggcgggagc cgaagcccgc
     1021 catcagcatt ttccctacac atggctcagt cgtctgccta tggcgccggg tcaccccggc
     1081 tggcacgctg ggccgacaag tgaaacgagg gcccatggct cgaggagaag taccgcgctc
     1141 agtggtgctt ctgggttccg agcgtggtac ttgtagtggc ggcgaatcat tggggaaccg
     1201 acggggttcc aaggagaggg gcatttcgat ggcggtaacg agggagtcgg ccgagtcggc
     1261 cgcgcgtccg ggctcggccc gcccgcgagc gaggatcttc aggaaggcgg cggccggagc
     1321 tctcacgctg tgctcggtcg cgctggccgt ggggatcacg ctgccgccgg cgcaggcttc
     1381 gatctctcag cctgcggtgg tgtcggagaa cccgtccgac accacccccc acctcatcat
     1441 ggggaccgcg ggggaggacg tccgcgcctt cgctcaggtc ggcaacacga tgtatgccgg
     1501 tggcctgttc aaccaggtcc aggactgggc ccggacgacc acctacgcca ggcagaactt
     1561 cgtcgccttc gaccgcgaga ccggggtcat ctccccgctc aacctcgcct tcaacgggcg
     1621 ggtcggtggc atcgaggcca ccgccgatgg aacggcgctg ttcatcgccg gcgccttctc
     1681 gacggtcaac gacatcaccc gccggggtct ggtcaagtac gacctggtca acaaccggat
     1741 cgacccgacc ttcctgccgg ggaacatgcg aacggtctct gacatcaagc tggccaacgg
     1801 ttcgctcatc gccgcgggga acttcaccgg gcatgtgcgg gcgctgaacc cgaccacggg
     1861 cgccaacctc ggcaccatca acatcacggt cgccggggtg gtcaacccgg acgacgagac
     1921 cagggtccga cacatcgcgg tctccccgaa cggcacccgg ttggtggcca ccggcaactt
     1981 cgccacggtc aacggccagg gtcgccggcg ggccttcatg ttgaacctgg gcccgaccgc
     2041 gacgctcagc acctggcacg ccccccgctt cgacgaagac tgctacgtgt tcgccaggcc
     2101 ggacagtgcg caaggcgtgg acttctctcc cgacggcacc tacttcgtga tcgtgtccac
     2161 cggagggccg accggcacca acgggatgtg tgatgctgca gcccggttcg agaccgccaa
     2221 tgtcagcgcc aatgccgcgc cgacctggat caactggact ggcggcgact ccctctacag
     2281 cgtggccatc accggaccgg cggtctacgt cggcggtcat caacgttggc tggacaaccc
     2341 cctgggcttc gactcggccg gtcctggcgc cgtctcccgc ccgggcatcg gcgccatcaa
     2401 ccccgtgacc gggagggcgc tcgcctggaa ccccaccaag tcccgcaacc acggcaccat
     2461 ggtcctctac cccacccccc aagggctctg ggtcggcagc gacggcgaag aattcgggca
     2521 cgaagaccac gcgggaatcg gcttcgcccc gctcggcgac gacgaggaca cgactgatcc
     2581 gacggtgggg gtggtgacgc cgcctggggg ggcggtctat gcccggaatc gggtggtgaa
     2641 cgccgatttc tcgtgtgcgg atgaggcggg cggttcgggt ctggcgtcct gtgtgggcac
     2701 c
//
LOCUS       DASTFV010000008         3123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_26922, whole genome shotgun sequence.
ACCESSION   DASTFV010000008 DASTFV010000000
VERSION     DASTFV010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26922"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VFP02_00105"
     CDS             complement(<1..1151)
                     /locus_tag="VFP02_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541451.1"
                     /translation="MRSSWHRRLAVAGVAAIGVVLLAAPPAAAHGAGTLSGLPLPRWQ
                     FAWGIAAVVIIAFFAVGSTWRRPRLDAAADGRPFPGWARPVTTVLGVVLRVVGVVVYV
                     VVVTAGLFGSEFPAANIAPIGVFITFWVGIQFVSMFLGDVWRVLSPFGTLALVGAWAR
                     ALVRREPLAPVESDEGASHWPAAAAVAGFVWLELAYHAPTTPMVLGSVALAYGVAELA
                     AAARWGRSWLRTGEGFAVLFSLFAAMAPLARDGRGGLKVRWPLSGLSRLVVRPGTLPL
                     LFVVLGAAIFDGVTRTTFWFDLTVERLGWGYTAVNTLGLAWTIGIVALVYLSVLRVVA
                     HVVDEDPDETAVRYAPILVPMAAAYTVAHYFSVLVLEGQGFWFLISNPYG"
     gene            1267..1581
                     /locus_tag="VFP02_00110"
     CDS             1267..1581
                     /locus_tag="VFP02_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541452.1"
                     /translation="MDLTPGTKLASTVCATEVIIVRPPSQTVALECGGAPMVAAEEAP
                     EEGARGSVDATRAEGTMLGKRYVHEASGLEVLCTKPGAGSLSAEGVALPLKEAKPLPS
                     SD"
     gene            1588..>3123
                     /locus_tag="VFP02_00115"
     CDS             1588..>3123
                     /locus_tag="VFP02_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET9541453.1"
                     /translation="MSILGNRVQRFEDPELLTGAGDYVDDVTLPADTAHVVYVRSPLA
                     YARIEAVDTSEALAAPGVLAVVTAADLDLADLEPEMMFLNPAMKRPLLARDVVRFVGE
                     PVAAVVAETRAQAVDAAEQLIIDYDPLDAVVDPERAREHGSTLFPDAETPLAMEIAGD
                     LVADFDGCEAVVEQRIVNQRVSAAPIEPRAAAGWWEDGRLIMWSSCQGAHPVRDKLAA
                     VYGLDPADVRVVVPHVGGSFGAKNGELPEELLIGDLARRVGRPVRWAETRSENMTGFV
                     HGRGQIQQVKIGGNRDGKVTAYSLSVIQDSGAYPMLGAVLPFMTRMMTSGVYAITNLG
                     FESASVVTNTTPMRPFRGAGRPEAAAAIERAMDLFAAEIEMDPAEVRRRNLLADDVYP
                     YSTPGGAVYDIGAYTTALDMALEASDYAGLRAEQVRRREAGDARQLGIGLSVYVEITA
                     FGGSGEFGTVTALGDGTVEIRTGATPFGQGHDTTWKMLATDRLGVPMEAIRVIHGDTD
                     DVAS"
BASE COUNT          501 a         1137 c         1124 g          361 t
ORIGIN      
        1 ccgtaggggt tggagatcag gaaccagaag ccctggccct ccaggaccag caccgagaag
       61 tagtgggcca cggtgtacgc ggcggccatg gggaccagga tcggggcgta ccggacggcc
      121 gtctcgtccg ggtcctcgtc gacgacgtgg gccacgaccc ggaggaccga caggtagacg
      181 agggccacga tgccgatcgt ccaggccagg cccagcgtgt tgaccgcggt gtacccccag
      241 cccaggcgct ccacggtgag gtcgaaccag aacgtcgtcc gggtgacccc gtcgaagatg
      301 gccgccccca ggacgacgaa cagcaacggc agggtgcccg gccgcacgac caggcgcgac
      361 aggcccgaca gcggccatcg caccttcagc ccgccgcggc cgtcccgcgc caacggggcc
      421 atggcggcga acagggagaa gaggacggcg aagccctccc ccgtgcgcag ccagctccgc
      481 ccccagcgag ccgcggcggc caactccgcc acgccgtagg ccagggccac gctgcccagg
      541 accatcggtg tggtgggcgc gtggtacgcc agctccagcc agacgaaccc ggccacggcg
      601 gccgcggccg gccagtgcga tgcgccctcg tcggactcga ccggagccaa tggctctcgg
      661 cggaccaggg cccgggccca ggccccgacc agggccagcg tcccgaacgg gctcagcacc
      721 cgccagacgt cacccaggaa catcgagacg aactggatcc cgacccagaa cgtgatgaac
      781 acgccgatcg gggcgatgtt ggcggccggg aactcgctgc cgaacagacc cgcggtgacg
      841 acgacgacgt agacgacgac cccgaccacg cggaggacga cgccgaggac cgtggtgacc
      901 ggtcgggccc atcccgggaa cgggcgcccg tccgccgcgg cgtcgagccg gggccgccgc
      961 cacgtcgacc ccaccgcgaa gaaggcgatg atcacgaccg cggcgatgcc ccacgcgaac
     1021 tgccaacggg gcaacggcag cccactcagc gtccccgccc cgtgcgccgc ggcgggaggc
     1081 gcggcgagca ggacgacgcc gatcgcggca acgccggcga cggcgagtcg gcggtgccag
     1141 gacgacctca tgtcgggcga ggctaggcag tccccccttc cggcccgatc agggcaggag
     1201 gtgaccggca gggaacagca cgatgtcgtt tcggcccgac tcggctgcgt cgggcaggat
     1261 ggggccgtgg atctgactcc gggaacgaag ctggccagca ccgtctgcgc cacggaagtg
     1321 atcatcgtcc gcccgcccag ccagaccgtg gccctcgagt gcgggggagc gccgatggtc
     1381 gccgcggagg aggctcccga agagggggct cgggggagcg tcgacgccac gcgcgccgaa
     1441 gggacgatgc tcggcaaacg ctacgtccac gaggccagcg gcctcgaggt cctgtgcacg
     1501 aagcccgggg cgggctccct ctcggccgag ggggtggccc tccccctcaa ggaggccaag
     1561 cccctcccga gctcggacta gcccggggtg tcgatcctcg gcaaccgggt ccagcgcttc
     1621 gaggacccgg agctcctcac gggcgcgggc gactacgtcg acgacgtcac cctccccgcc
     1681 gacacggccc atgtcgtcta cgtgcgctcc cccctggcct acgcccgcat cgaggccgtc
     1741 gacaccagcg aggccctcgc cgcgcccggt gtcctggccg tggtcaccgc ggccgacctc
     1801 gacctggccg acctcgagcc cgagatgatg ttcctgaacc cggccatgaa gcggccgctc
     1861 ctggcccggg atgtcgtccg cttcgtcggc gaaccggtcg ccgccgtcgt ggccgagacc
     1921 cgggcccagg cggtggacgc cgccgagcag ctgatcatcg actacgaccc gctggacgcc
     1981 gtcgtcgacc ccgagcgggc ccgcgagcac ggctcgaccc tcttcccgga cgccgagacc
     2041 ccgctggcca tggagatcgc cggtgacctg gtggccgact tcgacggctg tgaggccgtg
     2101 gtcgagcagc gcatcgtcaa ccagcgggtg tcggccgctc ccatcgagcc ccgcgccgcg
     2161 gcgggctggt gggaggacgg ccgcctgatc atgtggtcga gctgccaggg cgcccacccc
     2221 gtgcgggaca agctggcggc cgtctacggc ctcgatccgg cggatgtccg ggtcgtcgtg
     2281 ccccatgtcg ggggcagctt cggggccaag aacggcgagt tgccggagga gctgctcatc
     2341 ggcgacctcg cccgccgggt cggccgtccg gtgcgctggg cggagacccg ctcggagaac
     2401 atgaccgggt tcgtccacgg tcggggccag atccagcagg tcaagatcgg cggcaaccgg
     2461 gacggcaagg tcaccgccta ctcgctgtcg gtcatccagg acagcggggc ctaccccatg
     2521 ctcggcgcgg tcctgccgtt catgacccgg atgatgacca gcggggtcta cgccatcacc
     2581 aacctcggct tcgagtccgc gtcggtggtc accaacacca cgccgatgcg cccgttccgt
     2641 ggggccggtc ggccggaggc cgccgccgcc atcgagcggg ccatggacct gttcgcggcc
     2701 gagatcgaga tggatccggc cgaggtgcgc cggaggaacc tgctggccga cgacgtgtac
     2761 ccgtacagca cgccaggcgg ggcggtgtac gacatcgggg cctacacgac cgcgctggac
     2821 atggcgctcg aggcctcgga ctacgccggc ctgcgggccg agcaggtccg ccggcgagag
     2881 gcgggggacg cgcgccagct cggcatcggg ctgagcgtct acgtggagat caccgccttc
     2941 ggcggtagcg gcgaattcgg cacagtcacc gcgctggggg acggcaccgt cgagatccgg
     3001 accggggcca cgcccttcgg ccagggccac gacaccacat ggaagatgtt ggcgacagac
     3061 cgcctgggcg tgcccatgga ggcgattcgg gtcatccacg gggacaccga cgatgtggcc
     3121 tcc
//
LOCUS       DASTFV010000009         3269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_27676, whole genome shotgun sequence.
ACCESSION   DASTFV010000009 DASTFV010000000
VERSION     DASTFV010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27676"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFP02_00120"
     CDS             complement(<1..269)
                     /locus_tag="VFP02_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin-associated ferritin-like protein"
                     /protein_id="HET9541454.1"
                     /translation="MASEGLHESAEKLTAETIDYHRAMVSLMEELEAVDWYNQRVDAT
                     DDEDLAAVLAHNRDEEKEHAAMTLEWLRRRDPKLDQQLRTYLFSDG"
     gene            complement(1593..>3269)
                     /locus_tag="VFP02_00125"
     CDS             complement(1593..>3269)
                     /locus_tag="VFP02_00125"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HET9541455.1"
                     /translation="RRIADAYWEAVLDASPVYATFVGDHRYDDRMDDLSATAESAHLG
                     VVAALDQDVDILDPATLSAQGRVTRGLLRAEVESLRIRVELRLAELASDQNTGAHADL
                     LQIAPQTRASDARSADRLVERYRQAGRFLDQAAERFRAGLAAGRTPAAICIERSLNQV
                     DGYLASSLDTDPFVQLPPPQDTEGWDEDAWRDQLRQVTEEIIRPGFVRYRAVLADDLQ
                     PVARPDERPGLCHLDGGDEIYAALVRLHTTTDLSPQELHDIGIEEITERLPVEYAEIG
                     GRAFGLPDTEAVFDRLRSDPALRYATGDEIMADAADALGRAKAAMGDWFGRLPESDCA
                     IVPVPEYLAADAPAAYYFPPAGDGSRPGTYYVNTHNPGDKSRTEAESIGYHEAIPGHH
                     LQLAIAAELTDLPAFQRFGFGNTAYVEGWALYTERLADEMGLYSGDLDRLGMLSADAW
                     RAGRLVVDTGLHALGWTRRQAIDFLLEHTPLDEGEVTVEIDRYIGMPGQALAYKAGQR
                     EFFRLRAAARDQLGDRFDIKGFHDAVLAHGSVTLPILGDLVDEWVASLAA"
BASE COUNT          395 a         1110 c         1238 g          526 t
ORIGIN      
        1 ccgtcgctga agaggtaggt gcggagctgc tggtcgagct tcgggtcccg gcgccggagc
       61 cattccagcg tcatcgccgc gtgctccttc tcttcgtccc ggttgtgggc caggaccgcc
      121 gccaggtcct cgtcgtcggt ggcgtcgacg cgctggttgt accagtcgac ggcctccagc
      181 tcctccatca gcgagaccat ggcccggtgg tagtcgatgg tctcggccgt cagcttctcg
      241 gccgattcgt gcagtccttc actcgccatg gcctgattgc tagcacccgt ttcgagcgaa
      301 caggtagtga gacacgccga gctcgtgggt tcgagcgatc caatgtggcc gccgctccgt
      361 agtaggggag taagcgcgaa ctgacgccgg gccgggtggc cgtcctctcc ccctaggcca
      421 cccggttcgg cgtcggtccc gtccccgagg agtggctcaa gcccgctctg atcggggcga
      481 tgggtagggg tgccgaccgc cacgctgatc gacgcgcccg ccccggcggt ccgggcgacg
      541 cccggtgcgg tccgccgggt cgtcctcctc ggcctgctgc tgaacctcgg cctgatcgcg
      601 ttctgcgcgg cgacctacgg gggcggagcc gagtggttcg tgaagttcgg cgaagacggg
      661 gcctacacgc cctacgcccg agacgtgctg ggcgacgacc tgctcgtccc cctcgaggac
      721 ggtcacgacg ggcagggcta ctggctgcag gcccgggacc cgctcctggt ggacggggac
      781 cgcatggcgg ccacgttcga ccgcccggcc taccgggccc agcggatgct ctacccgacc
      841 ctggccgcgc ccttccacct cgtcggcgag gacgccgtcc tgtggggcct cgtcgtggtc
      901 aacctggtcg ccgtcgggtt cggcacgctc tgggccacgc ggctggccca ggccaccggg
      961 gcgcccgaac gggccgggtt ggccttcgcg ctgaacccgt tgatcgccat ctccctggtc
     1021 atggacttcg ccgacgccct ggcgttggcc ggcctggtcg gggtcgctct ggccgtgcac
     1081 cggcagcgct gggggtgggc ggtggccgcc ggcgtcgctg cggtgctggc caaggacgtg
     1141 acgggggtcg cccttgccgc actcgtgctg ctgtggcccg tcgccggtgc cgtccggcgg
     1201 cgctgggcaa tcgtcgtcgt ccccgcgttg gctgcgtcgg gatgggcggt gtatgtccgc
     1261 tggcgcctgg gttggccggc cagcgacatc gaggagttca cgcccgtccc cctgtggggg
     1321 tacgtcgacg cctaccggcg ggggtggagc caacacggca actggggtga cgcgatgttc
     1381 tctttgttcc tgctggcggc ggccgcggtg gtggtcgtgc gctggtggcg acgccgaaat
     1441 gtcgagatga cgatggccct gcccttcgcc ctgctggtgc cgttcctgac ggccacggtg
     1501 accgacctcg ccatcaactc gttccgggtc atcgccccgg ccctgacctt cggggtcatc
     1561 gactggtacg ccgagaccgc gggccgcgcc gatcaggcgg ccagtgacgc cacccattcg
     1621 tcgaccaggt cacccaggat gggcagcgtg accgagccgt gggccaggac ggcgtcgtgg
     1681 aagcccttga tgtcgaagcg gtcgcccagc tgatcccggg ccgcagcccg gagccggaag
     1741 aactcgcgct gccccgcctt gtaggccagt gcctggccgg gcatgccgat gtagcgatcg
     1801 atctcgacgg tgacctcacc ctcgtccagc ggggtgtgct cgagcaggaa gtcgatggcc
     1861 tggcgccggg tccaacccag ggcgtgcagc ccggtgtcga caacgaggcg cccggcccgc
     1921 caggcgtcgg cgctcagcat gccgagacgg tcgaggtcgc cgctgtagag gcccatctcg
     1981 tcggccaggc gctccgtgta gagggcccag ccctcgacgt aggccgtgtt gccgaacccg
     2041 aagcgctgga aggccggcag gtcggtgagc tcggcggcga tggccagctg caggtggtgg
     2101 ccggggatgg cctcgtggta gccgatgctc tcggcctcgg tccgtgactt gtcgcccggg
     2161 ttgtgggtgt tgacgtagta ggtgccgggc cggctgccgt cgcccgccgg cgggaagtag
     2221 taggcggcag gggcgtcggc ggccaggtac tcgggcacgg ggacgatggc gcagtcggac
     2281 tcgggcaggc gcccgaacca gtcgcccatc gccgctttgg cccggccgag ggcgtcggcg
     2341 gcgtcggcca tgatctcgtc gcccgtggcg tacctgaggg ctgggtccga acgcagtcgg
     2401 tcgaacaccg cctcggtgtc ggggaggccg aaggcccgcc ccccgatctc cgcgtactcg
     2461 acgggcaggc gctcggtgat ctcctcgatc ccgatgtcgt gcagctcctg ggggctgagg
     2521 tcggtggtgg tgtggaggcg gaccagcgcg gcgtagatct cgtcgccgcc gtcgaggtgg
     2581 cacagaccgg gacgctcgtc gggccgggcc accggctgga ggtcgtcggc cagcacggcg
     2641 cggtagcgga cgaacccggg gcggatgatc tcctcggtga cctggcgcaa ctgatcccgc
     2701 caggcgtcct cgtcccagcc ctcggtgtcc tggggcggtg ggagttggac gaacgggtca
     2761 gtgtcgaggg acgaggccag gtagccgtcg acctggttga gggagcgttc gatgcagatg
     2821 gcggcggggg tccgcccggc ggccagcccg gcccggaagc gctcggcggc ctggtcgagg
     2881 aagcgcccgg cctggcggta ccgctcgacc agccgatcgg cggaccgagc gtcgctggcc
     2941 cgggtctggg gggcgatctg caggaggtcg gcgtgggccc ccgtgttctg gtccgaggcc
     3001 agctccgcca ggcggagctc gactcggatg cggaggctct cgacctcggc ccgcaacagg
     3061 ccccgcgtca cccgaccctg ggcggagagg gtcgccgggt ccaggatgtc gacgtcctgg
     3121 tccagcgcgg cgaccacgcc gaggtgggcg gactcggccg tcgcggacag gtcgtccatt
     3181 cggtcgtcgt agcggtggtc gcccacgaac gtggcgtaga cgggcgaggc gtccagcacc
     3241 gcctcccagt aggcatcggc gatccggcg
//
LOCUS       DASTFV010000010         4520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_28447, whole genome shotgun sequence.
ACCESSION   DASTFV010000010 DASTFV010000000
VERSION     DASTFV010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28447"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..935
                     /locus_tag="VFP02_00130"
     CDS             99..935
                     /locus_tag="VFP02_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9541456.1"
                     /translation="MASGGKTSNWWTVWGVIILAWALFPLLWMTSLAFKSPDTFRSGS
                     PSFFPEDWTWDNFDTVFNDSLFTSALRNSFGIAIIATLLSVIVAMFAAYAIARLEFRG
                     KRLLLSMALAIAMFPQAALVGPLFNMWRNLGIYDTWLGLIIPYLTFALPLSIWTMSAF
                     FRQIPWEMEQAAQVDGATSWQAFRKVIVPLAAPGVFTTAILTFFFCWNEFLLAISLTS
                     TDRARTVPAALSFFTGQSQFQSPVTAVMAASVVVTVPIVIIVLIFQRRIVAGLTAGAV
                     KG"
     gene            949..2151
                     /gene="ugpC"
                     /locus_tag="VFP02_00135"
     CDS             949..2151
                     /gene="ugpC"
                     /locus_tag="VFP02_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET9541457.1"
                     /translation="MAEITLRNIVKKYGDGFPAVNDVSLDIADGEFMILVGPSGCGKS
                     TVLRMIVGLEDITDGEVTIGGERMNEKAPRDRHLAMVFQNYALYPHLTVFENIAFPLR
                     LAKDLSEDEIRAKVEEAAGTLELTEHLDRRPANLSGGQRQRVAMGRAIVREAQAFLFD
                     EPLSNLDAKLRGQMRTEILRMQRRMGTTTVYVTHDQVEAMTLGDRVALLRKGELQQVG
                     PPRELYEHPLNLFVAGFIGSPPMNFVPATLDGDQLQLPFVTFTLPEDMRAQVGDREHV
                     MVGIRPERFDDAEAVDGDKAAHGVTFDAEIDVVEWLGNEQYAYIPYEAPEMLEETLEE
                     LERDLDSERMRSQLVVALDPSSTIDAGTTAKLWFDPRHMHVFDVESGENLTNPASAPE
                     PVGADAGA"
     gene            complement(2164..3378)
                     /locus_tag="VFP02_00140"
     CDS             complement(2164..3378)
                     /locus_tag="VFP02_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HET9541458.1"
                     /translation="MADGGAEVEVLVRGGHPDFLDLPWTVALTQWDHERLVPMAHGVS
                     RHVVRFVRYDNRVYAIKETDAALAAREHRTLRTLLDVGLPVVESVGFVRDRGLGEVGA
                     LITRYLDYSLPYWYVLGREAVAAKEVLIDAAVVLLVRLHLAGVFWGDCSLSNVLFRRD
                     GGAMMTYLVDAETAEKRPSINDRLRAHDLEIAHENVVGGLLDLQAAERVPTDADPVAI
                     ADELRDRYEELWEELTRTDRFDSGERWRIDGRLRRINELGFDVEELAIRSADGGNVLD
                     ITPALVEEGHHARALQRSTGLRVQENQARRLLADIENFRARLEQDEGNPVPAAVGAAR
                     WLNEVYEPIIGAVPDELAGRLEPPELFHEMLDHRHAMAERAGREITNPEALADYLTTV
                     LPDRPEEKRLLE"
     gene            complement(3420..>4520)
                     /locus_tag="VFP02_00145"
     CDS             complement(3420..>4520)
                     /locus_tag="VFP02_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HET9541459.1"
                     /translation="VSLGGLVREAVFRALEDAQLTLADIDAVVLGKAPDLFEGVMKPE
                     LYLSDALGAVGKPMFRVHTAGSVGGTTAIVAASHVQAGKHKTVLAVAFEKQSEGNAQF
                     ALGSGKGASLGAGGAFAPFIRAYIQRSGAPEHIGWKVAVKDRLNALKNPYAHLKIEDI
                     SIEKVKASPMMWEPIRFLESCPSSDGACAMVLTDEAGGRAAAADGRPPAWILGTSSRS
                     EPPNFPGWDPVRPAACIQCADDVYAMAGITDPRRQLDTAELYVPFSWHEAIWLEGHGI
                     AAEGEGWKMIDDGSTELGGSFPINASGGVLSSNPIGASGLLRFAEAANQVRGAADEHQ
                     VEGAKTALAMAYGANSQYFSMWIVAERLQPFG"
BASE COUNT          676 a         1591 c         1534 g          719 t
ORIGIN      
        1 gccgtgtccg tgctcctctt cgccaccgtc gtgatcatcg cggtgatctt catcaagggc
       61 ttccgcaccg atctcagcca ggtcaggggt gatgcgtgat ggcgagcggc ggcaagacca
      121 gcaactggtg gacggtctgg ggcgtgatca tcctcgcctg ggcgttgttc ccgctcctct
      181 ggatgacctc cctggccttc aagagccccg acaccttccg cagcgggtcg ccgtcgttct
      241 tcccggagga ctggacctgg gacaacttcg acaccgtctt caacgactcg ctgttcacat
      301 cggccctgcg caactcgttc ggcatcgcca tcatcgccac gttgctgtcg gtgatcgtgg
      361 ccatgttcgc ggcctacgcc atcgcccgcc tcgagttccg gggcaagagg ctcctgctgt
      421 cgatggccct ggccatcgcc atgttccccc aggccgccct ggtcggcccc ctgttcaaca
      481 tgtggcgcaa cctgggcatc tacgacacgt ggctcggcct catcatcccg tacctcacct
      541 tcgccctccc gctgtccatc tggacgatgt cggccttctt ccgccagatc ccctgggaga
      601 tggagcaggc cgcccaggtc gacggggcca cgtcgtggca ggcgttccgc aaggtcatcg
      661 tgcccctcgc cgcccccggt gtcttcacca cggcgatcct gaccttcttc ttctgttgga
      721 acgagttcct gctcgccatc tccctgacct ccacggaccg ggcccgcacg gtgcccgcgg
      781 ccctctcgtt cttcaccgga cagtcgcagt tccagtcgcc ggtgacggcg gtgatggctg
      841 cgtcggtggt cgtcaccgtc ccgatcgtca tcatcgtgct catcttccag cggcgcatcg
      901 tcgctggcct gaccgccggt gccgtaaagg gctgacccaa ggagcgacat ggccgagatc
      961 accctgcgca acatcgtcaa gaagtacggc gacggcttcc cggcggtcaa cgacgtcagc
     1021 ctcgacatcg ccgacgggga gttcatgatc ctggtcggcc cgtcgggctg tgggaagtcg
     1081 accgtgctgc ggatgatcgt gggcctggag gacatcaccg acggcgaggt gaccatcggc
     1141 ggcgagcgga tgaacgagaa ggcgccgcgg gaccgccacc tggccatggt cttccagaac
     1201 tacgccctct atccgcacct caccgtgttc gagaacatcg ccttcccgct ccggctggcc
     1261 aaggacctgt ccgaggacga gatccgggcc aaggtcgagg aggccgccgg caccttggag
     1321 ctgaccgagc acctcgaccg gcggccggcc aacctgtccg gtggtcagcg tcaacgggtg
     1381 gccatgggcc gggcgatcgt ccgggaggcg caggcgttcc tcttcgacga acccctgtcg
     1441 aacctcgacg ccaagctccg gggtcagatg cggaccgaga tcctgcgcat gcagcggcgc
     1501 atggggacca ccaccgtcta cgtgacccac gaccaggtcg aggccatgac cctgggtgac
     1561 cgcgtggcgc tgctgcgcaa gggcgagctg cagcaggtgg ggccgcctcg cgagctctac
     1621 gagcacccgc tgaacctgtt cgtggccggg ttcatcggct ccccgcccat gaacttcgtg
     1681 cccgcaaccc tcgacggcga ccagctccag ctcccgttcg tcacgttcac gctccccgaa
     1741 gacatgcgcg cgcaggtcgg ggaccgggag cacgtcatgg tcggcatccg ccccgaacgg
     1801 ttcgacgacg ccgaggccgt cgacggcgac aaggccgccc acggcgtcac ctttgacgcc
     1861 gagatcgacg tggtcgagtg gttgggcaac gagcagtacg cctacatccc ctacgaggcc
     1921 cccgagatgt tggaggagac gctggaagag ctcgagcgcg acctcgacag tgagcggatg
     1981 cgcagccagc tcgtcgtcgc cctcgacccg tccagcacga tcgacgccgg cacgacggcc
     2041 aagctctggt tcgacccccg gcacatgcat gtcttcgatg tcgagagcgg cgagaacctc
     2101 accaacccgg cgtcggcgcc cgagcccgtg ggcgccgatg ccggcgcctg acccgacggt
     2161 cgttcactcc aggaggcgct tctcctcggg ccgatcgggc agcaccgtgg tcaggtagtc
     2221 ggcgagcgcc tcggggttgg tgatctcccg gcccgcccgt tcggccatgg cgtgacgatg
     2281 gtcgagcatc tcgtggaaga gctcgggcgg ctcgagccgg ccggccagct catcggggac
     2341 ggcgccgatg atcggctcgt agacctcgtt cagccaccgg gcggcgccca cggcggccgg
     2401 tacggggttc ccttcgtcct gctcgagccg ggcccggaag ttttcgatgt cggccaagag
     2461 ccggcgggcc tggttctcct ggacccggag gccggtgctc cgctgcaggg cccgggcgtg
     2521 gtggccctcc tcgacgagag ccggggtgat gtcgaggacg ttgcccccgt ccgccgagcg
     2581 gatggccagc tcctcgacat cgaagcccag ctcgttgatc cgacggagcc ggccgtcgat
     2641 ccgccaccgc tcaccgctgt cgaaccggtc ggtgcgggtc agctcctccc acagctcctc
     2701 gtagcggtcg cggagctcgt cggcgatggc caccgggtcc gcatcggtgg gcacccgttc
     2761 ggcggcctgc aggtcgagca gtcccccgac cacgttctcg tgggcgatct cgaggtcgtg
     2821 ggcccggagc cggtcattga tcgacggtcg cttctcggcc gtctcggcgt cgaccaggta
     2881 ggtcatcatc gccccgccgt cccggcggaa caggacgttg gagagcgagc agtcgcccca
     2941 gaacaccccg gcgagatgga ggcggacgag gagcacgacg gcggcgtcga tcagcacctc
     3001 cttggcggcc acggcctcgc ggcccagcac gtaccagtag ggcagcgagt agtcgaggta
     3061 ccgcgtgatc agggcgccga cctcgcccag gccacggtcc cggacgaagc cgaccgattc
     3121 gaccaccggg aggccgacgt cgagcagcgt gcgcagcgtc cggtgctcgc gcgcggcgag
     3181 ggcggcgtcg gtctccttga tggcgtagac ccggttgtcg tagcggacga agcgcacgac
     3241 gtgacgactg acgccgtggg ccatgggcac cagccgctcg tgatcccact gagtcagggc
     3301 cacggtccac ggcaggtcga ggaagtccgg gtgcccacca cggacgagga cctccacctc
     3361 cgcaccgccg tcggccatgg accgatcgtc gcacgggccg ggcgatcgac caacgcccgt
     3421 cagccgaagg gctggaggcg ttcggccacg atccacatgc tgaagtactg gctgttggcg
     3481 ccgtaggcca tggccagggc cgtcttcgcc ccctcgacct ggtgctcgtc ggccgcgccg
     3541 cggacctggt tggcggcctc ggcgaagcgc agcagacccg aggcgccgat ggggttgctg
     3601 gacaggaccc caccggaggc gttgatcggg aaggagccgc ccagctcggt ggagccgtcg
     3661 tcgatcatct tccagccctc gccctcggcg gcgatgccgt ggccctccag ccagatggcc
     3721 tcgtgccagg agaacggcac gtacagctcg gccgtgtcca gctgccggcg gggatcggtg
     3781 atgcccgcca tcgcgtagac gtcgtcggcg cactggatgc aggccgcggg gcgaaccggg
     3841 tcccatccag ggaagttcgg gggctcggag cgggacgagg tcccgaggat ccaggccggg
     3901 ggccgaccgt cggccgccgc cgctcggccg ccggcctcgt cggtcaggac catggcgcag
     3961 gccccgtccg aggacgggca ggactcgagg aagcggatgg gctcccacat catcggggag
     4021 gccttgacct tctcgatgga gatgtcctcg atcttgagat gggcgtaggg gttcttcagg
     4081 gcgttgagcc ggtccttgac cgccaccttc cacccgatgt gctcgggggc gccgctgcgc
     4141 tggatgtagg cccggatgaa gggggcgaag gccccaccgg cgccgagcga ggcccccttg
     4201 ccgctaccga gggcgaactg ggcgttgccc tctgactgct tctcgaaggc cacggccagg
     4261 accgtcttgt gcttgcccgc ctgcacgtgg gaggcggcca cgatggcggt ggtgccgccg
     4321 accgagcccg cggtgtgaac ccggaacatc ggtttgccca ccgccccgag ggcgtcggag
     4381 agatagagct ccggcttcat caccccctcg aacaggtcgg gggccttgcc cagcacgacg
     4441 gcgtcgatgt cggccaacgt gagctgcgcg tcctccagcg cccggaacac ggcttcccgc
     4501 acgaggccgc cgagggagac
//
LOCUS       DASTFV010000011         4346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_36818, whole genome shotgun sequence.
ACCESSION   DASTFV010000011 DASTFV010000000
VERSION     DASTFV010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36818"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="VFP02_00150"
     CDS             <1..1219
                     /locus_tag="VFP02_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9541460.1"
                     /translation="EVDYEAIGLWDDGPCDEAMEPLKIGLMTVFESPVLSLEDQAIAL
                     EASAEAFNARGGANGACIEVTTCDDGGNADQARACVRTVDEAGVVATVNDQGTAGQAD
                     VSPAMAEAGIPRVATNVVSEDWGDQNAYPLDASGTGVTFLLPQALIEEGISDIGLIRV
                     DLAAASALLGLLEDLYADEGATFPYDVPVPAGTTDFNQFILGAQDAGVGGVTLALGEQ
                     EAIQVVRAGQQLGTDLLIGSSLGTFSQATVAELGDFAEQMVFLWSFAPATFDLPVYEA
                     LRQDLAASGQESLQPANLTASPMRSWIGLYALLYLIRDAGMTDFTREGITQMLNQAED
                     VPMLDMFGGENWTPDADHPGAFSRGGINHWSTWTWDPAGETPDGLEGNFEEGSEILFD
                     DVLCGSPFGAETC"
     gene            1265..3997
                     /locus_tag="VFP02_00155"
     CDS             1265..3997
                     /locus_tag="VFP02_00155"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HET9541461.1"
                     /translation="MQSFLQFLIIGLGAGATYALFAQGAVLIYRGSGLVNFAQGAIGT
                     FAAYIAFVDLQGDRGWGTWPAIVVAVLAAGAVSLAFQALVLRALRNAAAIVRVIATIG
                     LLGLLQAIVLKRYGAANQPVDPYLPDDIYEWGGITVQQERIYLVAITLVVTVALWAWT
                     RYTRVGLAINASAQNERAVQTLGWSPDRLAALTWTIGGMLGGFAAVLAAPLTGLSAAT
                     FTIVVTVAGLGAALLGGFLSFPLTLLGGLIIGVGEAMATLYKGDIEDFLHQDLISGLS
                     RAPAFLVIFLVVVVRGRGLPLRSHIAERLPRLGTGEISVRGLLLASAIVLALLFGVMD
                     ESWAQATYISVATGVMVLSIVVLTGYAGQISLAQWALAGIGALIAGRFVHADLPVELA
                     IVLGVLLTIPVGLLFAVPALRTRGVNLAVVTLGLGFIVSEVVFANPNYLGDALDGGTR
                     IGQVKLFGMEVDAVNHPHAWAAVSLVAFVLLALLVANLRRSRTGRRLIAVRTNERAAA
                     SLGISVFGVKLYAFAVSAGLAAVAGILIGFRGQLITYTEFNVFASINSLGYAVIGGLG
                     YVLGAAFAAPNAVGGLGTRVIEDWIGLGNQWDLILGSLLVFLILIVHQNGIADLAVAH
                     GRGLWERLRLVAKRGEREPLPRAEVEPVAGATLSIEGLTVRFGSVVAVADVSMEVRPG
                     EVVGLIGPNGAGKTTLIDAVTGFVDVSEGAIALDDRRVDRMNATQRARLGLRRSFQSL
                     ELFEDVSVEENIRAGSDQRTSRLSWVSDLFWPGRHDLPPTAVAAVLEFELEPHLDQTP
                     EELPYGRRRLVGIARTVASGPSVVLLDEPAAGLDENESADLARLIRRLADERNMAVLL
                     VEHDVGLVMSTCDRIVVIDFGRVIGSGTPEEIRGDHAVRDAYLGHPDEVAEAHT"
     gene            3994..>4346
                     /locus_tag="VFP02_00160"
     CDS             3994..>4346
                     /locus_tag="VFP02_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9541462.1"
                     /translation="MTDTLIEAKSLSAGYGKMAVVRDVDLRVEAGEVVALIGPNGAGK
                     TTTLLTLAGELAPLEGEVHFLGTPTRSPMHARCRNGLGYVTEERSVIMNMSVADNLKL
                     AAVAPTVAFGYFPAL"
BASE COUNT          557 a         1611 c         1486 g          692 t
ORIGIN      
        1 cgaggtcgac tacgaggcca tcgggctgtg ggacgacggc ccgtgtgacg aggcgatgga
       61 gccgttgaag atcgggctca tgaccgtctt cgagtcgccg gtcctgtccc tggaggacca
      121 ggccatcgcc ctcgaggcct cagccgaagc gttcaacgcc cgcggtggtg ccaacggcgc
      181 ctgcatcgag gtcacgacct gcgacgacgg cggcaacgcc gaccaggcgc gggcgtgcgt
      241 acgaactgtc gacgaggccg gtgtcgtggc cacggtgaac gaccagggca ccgccggcca
      301 ggccgacgtg tcacccgcca tggcggaggc cgggatcccc cgcgtcgcca ccaacgtcgt
      361 gtccgaggac tggggcgatc agaacgccta cccgctcgac gcctccggca ccggggtgac
      421 cttcctcctg ccccaggcgc tcatcgagga ggggatcagc gacatcggcc tcatccgggt
      481 cgacctggct gctgcctccg cgctcctcgg cctcctcgag gacctgtacg ccgacgaggg
      541 ggcgacgttc ccctacgacg tcccggtccc ggccgggacc acggacttca accagttcat
      601 cctcggcgcc caggacgccg gcgtcggcgg cgtcaccctg gcccttggcg agcaggaggc
      661 gatccaggtc gtgcgggccg gccagcagtt gggcaccgat ctgctcatcg gctccagcct
      721 cggcacgttc tcgcaggcca ccgtcgccga gctcggcgac ttcgccgagc agatggtgtt
      781 cctctggtcg ttcgcgccgg cgaccttcga cctgcccgtc tacgaggccc tgcgccagga
      841 cctcgcggcg tccggtcagg agtcgctcca gcccgcgaac ctcacggcca gcccgatgcg
      901 ttcgtggatc gggctctacg ccctgctcta cctgatccgc gacgccggca tgaccgactt
      961 cacccgggag ggcatcaccc agatgctgaa ccaggccgag gacgtcccga tgctggacat
     1021 gttcggcggc gagaactgga ccccggacgc cgaccacccg ggcgccttct ctcggggcgg
     1081 catcaaccac tggtcgacgt ggacgtggga tcccgcgggg gagaccccgg acgggctcga
     1141 gggcaacttc gaggagggct cggagatcct cttcgacgac gtgctctgcg gctcgccgtt
     1201 cggtgcggag acctgttgag cccgcatccg gcctgacccg ccccgccccg gaggcccgtg
     1261 acccttgcag tcgttcctgc agttcttgat catcggcctc ggggcggggg ccacctacgc
     1321 cctgttcgcc cagggcgccg tcctcatcta ccgcggttcg ggcctggtca acttcgccca
     1381 gggggcgatc gggaccttcg ccgcctacat cgccttcgtc gacctgcagg gggaccgggg
     1441 ttgggggacc tggcccgcca tcgtcgtcgc cgttctggcc gccggtgcgg tgtcgctggc
     1501 gttccaggcc ctcgtcctgc gggccctgcg caacgcggcg gcgatcgtcc gggtcatcgc
     1561 caccatcggg ctgctcggcc tcctccaggc gatcgtcctg aagcgctacg gcgccgccaa
     1621 ccagcccgtc gacccctatc tccccgacga catctacgag tggggcggca tcacggtcca
     1681 gcaggagcgg atctacctcg tcgccatcac cctcgtcgtc accgtcgcgc tctgggcctg
     1741 gacccggtac acccgggtcg gcctggcgat caacgccagc gcccagaacg aacgggcggt
     1801 gcagaccctc gggtggtcgc ccgaccgcct ggctgccctc acctggacga tcggcgggat
     1861 gctcggcggg ttcgccgccg tcctcgccgc tccgctcacc ggcctgtcgg cggccacctt
     1921 cacgatcgtg gtcaccgtcg ccggattggg ggccgccctg ctcggcggct tcctctcgtt
     1981 cccgctcacc ctgctcgggg gcctgatcat cggcgtcggc gaggcgatgg ccaccctcta
     2041 caagggcgac atcgaggact tcctccacca ggacctcatc tccgggctca gccgcgcgcc
     2101 ggcgttcctc gtgatcttcc tcgtggtcgt cgtccggggt cgaggcctcc ccctgcgctc
     2161 gcacatcgcc gaacggctgc cccggctcgg gacgggcgag atcagcgtgc ggggactgct
     2221 gctggcgtcg gcgatcgtcc tcgccctgct cttcggggtg atggacgagt cgtgggctca
     2281 ggccacctac atctcggtgg ccaccggggt gatggtgctc tccatcgtgg tcctgaccgg
     2341 gtacgcgggg cagatctcgc tcgcccagtg ggctctcgcc ggcatcggcg ccctgatcgc
     2401 cggccgcttc gtgcacgccg acctccccgt cgagctggcg atcgtcctcg gcgtcctgct
     2461 gacgatcccg gtgggcctgc tcttcgccgt ccccgccctg cggacccgcg gggtgaacct
     2521 cgcggtggtc accctcggcc tcgggttcat cgtgtcggag gtggtcttcg ccaaccccaa
     2581 ctacctgggc gacgccctcg acggtggcac ccggatcggc caggtgaagc tcttcgggat
     2641 ggaggtcgac gccgtcaacc acccccacgc ctgggccgcg gtcagcctcg tcgccttcgt
     2701 gctgctggcc ctgctcgtcg ccaacctgcg ccgctcgcgc accggccgtc gactcatcgc
     2761 cgtgcggacg aacgagcgcg ccgccgcctc cctcggcatc tcggtgttcg gcgtgaagct
     2821 ctacgccttc gccgtctcgg ccggcctggc cgccgtggcc gggatcctga tcgggttccg
     2881 gggccagctc atcacctaca ccgagttcaa cgtcttcgcc tcgatcaaca gcctcggcta
     2941 cgccgtcatc ggcggcctcg gctacgtgct cggtgccgcc ttcgccgccc ccaacgcggt
     3001 cgggggcctc ggcacccggg tgatcgagga ctggatcggc ttggggaacc agtgggatct
     3061 catcctggga tccctgctcg tgttcctgat cctgatcgtg caccagaacg gcatcgccga
     3121 cctcgcggtc gcccacggtc ggggcctgtg ggagcggctc cgcctcgtgg cgaagcgagg
     3181 cgagcgcgag cccctgcccc gggccgaggt cgagccggtg gcgggagcga cgctctcgat
     3241 cgagggcctg accgttcgct tcggctcggt ggtggccgtc gccgacgtgt cgatggaggt
     3301 gcgcccgggg gaggtcgtcg gcctgatcgg gccgaacggc gcgggcaaga cgaccctcat
     3361 cgacgccgtg accggcttcg tggacgtctc cgagggcgcg atcgccctgg acgaccgacg
     3421 cgtcgaccgg atgaacgcga cgcagcgggc ccggctcggc ctgcggcgct cgttccagtc
     3481 cctcgagctg ttcgaggacg tcagcgtcga ggagaacatc cgggcgggca gcgaccagcg
     3541 gacctcgagg ctctcgtggg tgagcgacct gttctggccg ggccgccacg acctgccgcc
     3601 cacggcggtg gccgccgtgc tcgagttcga gctcgagccg cacctggacc agaccccgga
     3661 ggagctgcct tacggccggc gccggctcgt cggcatcgcc cggaccgtgg cgtcggggcc
     3721 gtcggtggtc ctgctggacg agcccgccgc cggcctcgac gagaacgagt ccgcggacct
     3781 ggcccggctc atccggcggc tggccgacga gcgcaacatg gcggtgctcc tcgtcgagca
     3841 cgacgtgggc ctggtgatgt ccacctgcga ccgcatcgtg gtgatcgact tcggcagggt
     3901 catcggcagc ggcacgcccg aggagatccg cggtgaccac gcggtgcggg atgcctacct
     3961 cggccacccc gacgaggtcg cggaggcgca cacgtgaccg acaccctcat cgaggcgaag
     4021 agcctgtcgg cgggctacgg caagatggcc gtggtccgcg acgtcgacct ccgcgtcgag
     4081 gccggcgagg tcgtggccct catcggcccg aacggcgccg gcaagacgac gacgctgctc
     4141 accctcgccg gcgagctggc gccgctggag ggggaggtcc acttcctcgg tacgccgacc
     4201 cggtcgccga tgcacgcgcg gtgccggaac ggcctcggct acgtcaccga ggagcgctcg
     4261 gtgatcatga acatgtccgt cgccgacaac ctgaagctgg ccgccgtcgc ccccacggtc
     4321 gccttcgggt acttccctgc gctcga
//
LOCUS       DASTFV010000012         3142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_46243, whole genome shotgun sequence.
ACCESSION   DASTFV010000012 DASTFV010000000
VERSION     DASTFV010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46243"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFP02_00165"
     CDS             complement(<1..183)
                     /locus_tag="VFP02_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541463.1"
                     /translation="MADPRDLLDLAVTAATDAADLLRSRRPDPEAIATKSSVTDLVTD
                     LDRAAETRIIDLLIGAR"
     gene            236..1258
                     /locus_tag="VFP02_00170"
     CDS             236..1258
                     /locus_tag="VFP02_00170"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HET9541464.1"
                     /translation="MPAPPTVDLRSDTMTRPTPAMRRAMAEAEVGDDGWGEDPTVRRL
                     EEVFADRLGKAAGLFVPSGTMANQVALRTLAGPGKMVVAGRRQHVVMSENGAAGMNGA
                     FQLYTVDDDEGRLDLAATEDAVAGDVGHSPEVAVIAVENTHMVAGGRATPAEALDAVA
                     ALGPPVHLDGARLFNAEVALGVPVARLAAAATTVTCCLSKGLGAPVGSVLAGPTELMA
                     EARVQRRRLGGGMRQAGILAAAGLLALDTMVDRLADDHARARRLAEAVATSLPDAIDP
                     ARVQTNVVVFSHPDPGAVVAHLAGEGVLAAPLGRGAVRLVTHLDVDDGGIDRAVEVLA
                     RLPTPH"
     gene            complement(1261..1515)
                     /locus_tag="VFP02_00175"
     CDS             complement(1261..1515)
                     /locus_tag="VFP02_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541465.1"
                     /translation="MAVHRPSLLARTLTVASVLAAGACGGLIGWAVVDLQCTGDCTVP
                     SGLGALVGALLAAVGTALICVLAIQAMAEWNQQEGRRRAD"
     gene            1555..2277
                     /locus_tag="VFP02_00180"
     CDS             1555..2277
                     /locus_tag="VFP02_00180"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HET9541466.1"
                     /translation="MRALLILPTFQEALNIVEVLDRVRAATPDVHILVVDDESPDGTA
                     DLAEEHGAVVGDVEVLRRAEKSGLGSAYRDGFRWGLERGFDVLLEMDSDLSHDPAALP
                     SLLGAVEHGADLVIGSRYVPGGSIPAWSWHRRQLSRWGNRYAAGVLGLAVNDSTAGFR
                     AYRATMLRQVDLDGVAADGYGFQIEMTYEVVRRGGSVVEVPIAFADRLRGTSKMSSRI
                     VVEALALVTWWGLRDRLLRRRP"
     gene            complement(2351..2566)
                     /locus_tag="VFP02_00185"
     CDS             complement(2351..2566)
                     /locus_tag="VFP02_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET9541467.1"
                     /translation="MLLAPAASAQYVDPGIVTRDDPGATVDDGTALPRTGSDTNTLWR
                     VGVVLLAAGGILVLTTRKHTAKVHVNA"
     gene            complement(2716..>3142)
                     /locus_tag="VFP02_00190"
     CDS             complement(2716..>3142)
                     /locus_tag="VFP02_00190"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET9541468.1"
                     /translation="YPDGTRTAEDAARAIGVDVAQIVKSLVFDLVPANGEVRPVIALV
                     SGANRLDEAKLATASGAERVGRVDADRVRAVTGFPIGGVPPFGHPTSIPTFVDPDLLD
                     HAEVWAAGGTPQVVFGIAPAALVVATEGDVRDLAASAHP"
BASE COUNT          422 a         1118 c         1160 g          442 t
ORIGIN      
        1 cctcgcgccg atgagcaggt cgatgatccg ggtctcggct gcccggtcga gatcggtgac
       61 caggtcggtc accgaggact tggtggcgat ggcctcggga tcggggcggc ggctgcgcag
      121 caggtcggcc gcatcggttg ccgcggtcac cgccaggtcg agcaggtccc tggggtcggc
      181 caccgggtca ggctggccga cggccacgcc ggacgccaac cgttaccgtc gcaccgtgcc
      241 cgctccgccg accgtcgacc tccgttcgga cacgatgacc cgcccgacgc cggccatgcg
      301 ccgggcgatg gccgaggccg aggtgggcga cgacggctgg ggcgaggacc cgaccgtccg
      361 tcgcctcgag gaggtgttcg ccgatcgcct gggcaaggcc gccggcctct tcgtgccctc
      421 ggggaccatg gcgaaccagg tggccctgcg cacgctggcc gggccgggga agatggtcgt
      481 ggccgggcgc cgccagcacg tcgtcatgtc cgagaacggc gcggcgggga tgaacggggc
      541 gttccagctc tacaccgttg acgacgacga gggccggctg gacctggcgg ccaccgagga
      601 cgccgtcgcc ggcgacgtcg ggcattcgcc cgaggtggcc gtgatcgccg tcgagaacac
      661 ccacatggtg gccggtggtc gggccacgcc cgccgaggcg ctcgacgccg tcgccgcgct
      721 gggcccaccg gtgcacctcg acggcgcccg actgttcaac gccgaggtcg ccctcggtgt
      781 ccccgtggcc cggctggcgg cggcggccac gaccgtgacc tgctgcctga gcaaaggcct
      841 cggcgcgccg gtcgggtcgg tcctggccgg tccgaccgag ctcatggccg aggcccgcgt
      901 gcagcgacgg cgcctcgggg gcgggatgcg acaggcgggc atcctggccg ccgccggctt
      961 gctggccctg gacacgatgg tcgaccgcct ggccgacgat catgcgcggg ctcgccgcct
     1021 cgcggaggcg gtggccacca gccttcccga cgccatcgac cccgcgaggg tccagacgaa
     1081 cgtcgtcgtc ttctcccacc ccgatcccgg tgccgtcgtg gcccacctgg cgggcgaagg
     1141 ggtcttggcc gcgcccctgg ggcgaggggc cgtgcggctg gtgacccacc tggacgtcga
     1201 cgatggcggc atcgaccgcg cggtcgaggt gctggcccgg ctcccgacac cgcactgagg
     1261 tcagtcggct cggcgccggc cttcctgctg gttccactcg gccatcgcct ggatggccag
     1321 cacacagatc agggcggtcc cgacggcggc cagcagggcg ccgacgaggg cgccgaggcc
     1381 gctcgggacg gtgcagtctc cggtgcactg gaggtcgacg acggcccaac cgatgagccc
     1441 gccacaggcc ccggcggcga gcacggaggc gacggtcagc gtgcgggcca gcaacgacgg
     1501 tcggtgcaca gccatccggc gggagcgtag gtccacctct ctacgatgcg accgatgcgc
     1561 gccctcctga tcctcccgac ctttcaggaa gccctcaaca tcgtcgaagt cctcgaccgc
     1621 gtgcgggcgg cgacgccgga cgttcacatc ctcgtcgtcg atgacgagag cccggacggc
     1681 acggccgacc tggccgagga gcacggagcc gtggtcggcg acgtggaggt cctacgtcgg
     1741 gccgagaagt ccgggctggg ctcggcctac cgggacgggt tccgttgggg cctcgagcgg
     1801 ggtttcgacg tcctgctgga gatggacagc gacctctccc atgacccggc cgccctgccc
     1861 tccctcctgg gagccgtcga gcacggcgcc gacctggtca tcggcagtcg ctatgtcccc
     1921 ggcgggtcga tcccggcctg gtcgtggcac cgccgccagc tctcccgctg gggcaaccgt
     1981 tacgccgccg gcgtcctcgg cctggccgtg aacgactcga ccgccggttt ccgcgcctac
     2041 agggccacga tgctgcgtca ggtcgacctc gacggtgtgg ccgccgatgg ctacggcttc
     2101 cagatcgaga tgacctacga ggtcgtccgt cggggcgggt ccgtggtcga ggtgcccatc
     2161 gccttcgccg accggctgcg gggcacgtcc aagatgtcga gccgcatcgt ggtcgaggcg
     2221 ctggccctcg tcacgtggtg ggggctccgg gaccgcctgc tgcgtcgccg tccctgatgg
     2281 cccgcgtccc cgggcgcgaa ggagcccggg ccggggcccg ggctcgttct accgatccga
     2341 gaggcttggc tcaggcgttg acgtggacct tggcggtgtg cttgcgggtg gtcaggacca
     2401 ggatcccacc agccgccagc aggacgacgc cgactcgcca gagcgtgttg gtgtccgaac
     2461 cggtccgggg gagcgcggtg ccgtcgtcga cggtggcgcc cggatcatcc cgggtgacga
     2521 tgccgggatc gacgtactgg gccgaggccg caggggccag gagcaccagt aacgcgaatg
     2581 cgatgccgat cactgcgaga gaacgacgga ccatggtgga ttcccctccg gttcgggctt
     2641 gcactgcagc caaatcatga cccacgacgg ggctggtgta aatcatactc tgaattcggc
     2701 ttccggacgt gaatctcagg ggtgggccga cgcagcgagg tcgcggacgt caccctccgt
     2761 ggccacgacc agggcggccg gagcgatccc gaagacgacc tgcggcgtcc ccccggcggc
     2821 ccacacctcc gcgtggtcga gcaggtccgg gtccacaaac gtgggtatcg acgtcgggtg
     2881 gccgaacggg ggtacgcccc cgatgggaaa gccggtgacc gcccgcaccc ggtcggcatc
     2941 gactcggccg acgcgctcgg ccccgctcgc cgtcgccaac ttggcctcgt ccaggcggtt
     3001 ggcgccgctg accagggcga tcaccggtcg gacctcgccg ttggctggca ccaggtcgaa
     3061 gaccagcgac ttcacgatct gggcgacgtc gacgccgatg gcccgagcgg cgtcctcggc
     3121 ggtgcgggtc ccgtcggggt ac
//
LOCUS       DASTFV010000013         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_47817, whole genome shotgun sequence.
ACCESSION   DASTFV010000013 DASTFV010000000
VERSION     DASTFV010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47817"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VFP02_00195"
     CDS             complement(<1..607)
                     /locus_tag="VFP02_00195"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HET9541469.1"
                     /translation="MADALFRPDGPMTFVPTSAAVGPWDKGIVHGAAVSALFAGRLAP
                     AGFTPARLTIEFLTAVPMAPLTLDCSEPRGGRRVQRQEAVLSCDGREVAAARSVLVRQ
                     GELDLPEKTRDHPSPFDPAEAPALDEPNRDATKAVGWDSFDSTSLIWAPTRVEGDRRI
                     HAWIRLAVPVVEGTDVRGVELAVVAADYAQAAVNRQLSMAEW"
     gene            647..1927
                     /locus_tag="VFP02_00200"
     CDS             647..1927
                     /locus_tag="VFP02_00200"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9541470.1"
                     /translation="MQMDDMILVSIDDHMVEPPDMYDNHVPAKWKDVVPKVVRNEAGV
                     DEWEFQGEKTSTPFGMAATVGWPREEWGFNPGAYSELRPGCFDVHERVKDMNANGILA
                     SMNFPTMAGFNARTFTEAGDKEIALVMLRAYNDWAIEEWAGSYPGRFIPLGLVPMWDI
                     DLAVEEVHRLAKKGCRSISFLETPHVQGFPSFLSGYWDPMMQALCDENMILSLHIGAG
                     FEVINRPKEAPIDHLMVLACQISAITAQDLLFGPTLRKFPDLKVALSEGGIGWIPFYF
                     DRIDRHFENQSWLHDGDDFGGKLPSEVFREHILACYITDPSGLLLRDRIGIDNISWEC
                     DYPHTDTTWPESPEQAWGELQDAGCTDEEIHKLTWENACRFFDWDPFEHTKREEATVG
                     ALRAQARAADVDVTRMPRAEWKKQNEAAGIGVLT"
     gene            complement(1928..2317)
                     /locus_tag="VFP02_00205"
     CDS             complement(1928..2317)
                     /locus_tag="VFP02_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9541471.1"
                     /translation="MSDEEQIRDLLARYCLTLDLDDVDGWVALFTPDATYQVYGRAFE
                     GHDGLRRMMGGAPGGLHLGGPPVIERVDDDHATTQRNLLFVERVSGTMRSAVYDDELV
                     RTPEGWRIGSTRCRFIVADGLADRPAD"
BASE COUNT          396 a          841 c          872 g          343 t
ORIGIN      
        1 accactcggc cattgagagt tggcggttga cggcggcctg ggcgtagtcg gctgcgacga
       61 cggcgagctc gacgccgcgg acatcggtgc cctcgacgac ggggacggcg aggcggatcc
      121 aggcgtggat ccgacggtcg ccctcgaccc gcgtcggcgc ccagatcagg ctggtgctgt
      181 cgaagctgtc ccagcccacg gccttggttg cgtcccggtt gggctcgtcg agggcaggcg
      241 cctccgccgg gtcgaacggg ctggggtggt cgcgggtctt ctcggggagg tcgagctcgc
      301 cctggcggac gaggaccgac cgggccgcgg ccacctcgcg accgtcgcag gacagcacgg
      361 cctcctggcg ctgcacccgc cgacccccgc ggggctcgct gcagtcgagg gtcaacgggg
      421 ccatggggac cgcggtcagg aactcgatgg tcaagcgggc cggcgtgaac ccggcgggcg
      481 ccagccggcc ggcgaagagg gcgctgaccg cggcgccgtg gacgatgccc ttgtcccacg
      541 gaccgactgc tgcggaggtc ggcacgaacg tcatcggccc atcgggccgg aagagggcat
      601 ccgccatcgg tggaccctaa accgcactcg cgcgaggctg ggaaccatgc agatggacga
      661 catgatcctg gtgagcatcg acgaccacat ggtcgagccc cccgacatgt acgacaacca
      721 tgtccctgcg aagtggaagg acgtggtgcc caaggtcgtc cgcaacgagg ccggcgtcga
      781 cgagtgggag ttccaggggg agaagacgtc caccccgttc ggcatggccg ccaccgtggg
      841 ctggccccgg gaggagtggg gcttcaaccc cggcgcgtac agcgagctgc ggcccggttg
      901 cttcgacgtg cacgagcggg tcaaggacat gaacgccaac gggatcctgg cgtccatgaa
      961 cttcccgacc atggccggct tcaacgcccg gaccttcacc gaggccggcg acaaggagat
     1021 cgccctcgtc atgctccggg cctacaacga ctgggccatc gaggagtggg ccggcagcta
     1081 tccgggccgg ttcatccccc tcggcctggt cccgatgtgg gacatcgacc tcgccgtcga
     1141 ggaggtgcac cggctggcga agaagggttg ccgttccatc agcttcctcg agacgccgca
     1201 cgtgcagggg ttcccgagct tcctctccgg gtactgggac ccgatgatgc aggccctgtg
     1261 tgacgagaac atgatcctct cgctgcacat cggggccggc ttcgaggtga tcaaccggcc
     1321 gaaggaggcc ccgatcgacc acctgatggt cctggcctgc cagatcagcg ccatcacggc
     1381 ccaggacctg ctgttcgggc ccacgctccg gaagttcccc gacctcaagg tggcgctctc
     1441 cgaggggggc atcggctgga tccccttcta cttcgaccgg atcgaccgcc acttcgagaa
     1501 ccagagctgg ctgcacgacg gcgacgactt cggcggcaag ctgccgtcgg aggtgttccg
     1561 cgagcacatc ctcgcctgct acatcaccga cccgtccggg ctgctgctgc gggaccgcat
     1621 cgggatcgac aacatctcgt gggagtgcga ctacccccac accgacacca cgtggccgga
     1681 gtcgcccgag caggcctggg gcgagctcca ggacgcgggc tgcaccgacg aggagatcca
     1741 caagctcacc tgggagaacg cctgccgctt cttcgactgg gatccgttcg agcacaccaa
     1801 gagggaagag gcgacggtcg gggccctgcg agcgcaggcc cgggcggccg acgtggacgt
     1861 cacccggatg ccccgcgccg agtggaagaa gcagaacgag gccgccggca tcggcgtcct
     1921 cacctgatca gtcggccggg cggtccgcga ggccgtcggc cacgatgaag cggcagcggg
     1981 ttgacccgat gcgccagccc tccggagtgc ggaccagctc gtcgtcgtag acggcgctgc
     2041 gcatcgtgcc cgacacccgc tcgacgaaca gcaggttgcg ctgcgtggtg gcgtggtcgt
     2101 cgtcgacccg ctcgataacc ggcgggccgc ccaggtggag gccgcccggc gcgccaccca
     2161 tcatgcggcg gagcccgtcg tggccctcga aggcgcggcc gtacacctgg taggtcgcgt
     2221 cgggcgtgaa cagcgccacc cagccgtcga cgtcgtcgag gtcgagggtc aggcagtagc
     2281 gggccagcag gtcgcggatc tgctcctcgt cgctcacgag cggggcagtc ccagcagctg
     2341 gcggccgacg aggccgcgca tgatctccga cgcgccaccg gagatggtca tggcccgccc
     2401 ccagaggaag ttgtggtacc agcggtcggt cacgtcgacg ccggcgtcgt cc
//
LOCUS       DASTFV010000014         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_51049, whole genome shotgun sequence.
ACCESSION   DASTFV010000014 DASTFV010000000
VERSION     DASTFV010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51049"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..>2419
                     /locus_tag="VFP02_00210"
     CDS             51..>2419
                     /locus_tag="VFP02_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HET9541472.1"
                     /translation="MGPPTPALPDLHVTAGPLTPHSVSLPDPVLIGHGVACGLRIDHP
                     SIAAAHALVESAGGGWTVSAVAIGATTALDGEPVSAPTPLRPGAVLSMGAVDLQLHVR
                     TRPALVPPPRGPVHRAAPTGAPIPDPPPAPPERPAPPPPPSPPGPASLLLPVAVGVIL
                     ALTVHPAAGLVTAATPILAVGAHLDARRRHRRTLRRDDAARAEEVERHRQQLATHQHG
                     VAARERTRFPTPVEALAQARSGIRLWAVREGDPTIRQVAVGWTDDAVRAPAIVNLDGD
                     RRIGVAGPGPWADAVARALVIQVTARAGPTAWALSVPAEPAWRFARWLPHQQGSGAAL
                     SVGHDGAGPGIEVADHRAQLTDRCAVVLTWSPDGARLWAPGHGDGRPVHPFLASVELA
                     EDYGRASARWTDPDPAGAALPDRLGLASLLTDAAGASEDGRRRLAAPLGVGVDGVVTV
                     DLVVDGPHALVAGTTGAGKSELLRTWVTALAHRHPPTAVAFVLVDYKGGSAFDACARL
                     PHVTGVVTDLDAGLGERLLVGLRAEIREREAGLRDAGVGDLRDLVDGPPRLVVVVDEF
                     ATLAAELPDFLGALVDVARRGRSLGVHLVLATQRPAGAVNDDIRANTALRLCLRTLDA
                     ADSQDVLGGPEAALLPADRPGRAWLRQGQGLRLLQVAGGSGPDRPPNGPGLAVRRVGD
                     PWPDGDEAGPVELDRLVDEVAGSWGDRPRPTPAWRPPLPDHLRLKRQIPARRDPAPLV
                     LGQADRPEERTQPPLTWSPTSGHLVVLGGRRSGRTTAVRTTVLGLAGSRS"
BASE COUNT          249 a          973 c          889 g          308 t
ORIGIN      
        1 cgcgggtacc actggccgcc tgtgggttgg ccgacggggc gacggtcgcc gtgggccctc
       61 cgacccctgc cctccccgac ctccacgtga cggccgggcc cctcaccccg cactcggtgt
      121 cgctcccgga cccggtcctg atcgggcacg gcgtcgcctg cgggctccgc atcgatcacc
      181 cctcgattgc tgccgctcac gccctcgtcg agtcggccgg gggtgggtgg acggtcagcg
      241 cggtcgccat cggggcgacc acggcgttgg acggcgagcc cgtgagtgct cccaccccac
      301 tccggccggg cgccgtgctg tcgatgggcg ccgtggacct ccagctccac gtccggaccc
      361 gtcccgccct ggtgcccccg ccccgagggc ctgtccaccg agcggcgccc acgggggcgc
      421 cgatcccgga tccacccccg gcgccaccgg agcgtccggc cccgcccccg cccccgagtc
      481 cgcctggtcc cgcctcgctg ctcttgccgg tcgccgtggg cgtgatcctg gccctgaccg
      541 tccacccggc cgccggcctg gtcaccgcgg cgaccccgat cctggcggtg ggcgcccacc
      601 tcgacgcccg gcgtcgtcac cggcgcaccc tgcggcgcga cgacgcggcg cgggcggagg
      661 aggtcgaacg ccaccggcag cagctggcga cgcaccagca cggcgtcgcc gcccgcgagc
      721 ggactcgctt cccgaccccc gtcgaggccc tcgctcaggc tcgctccggc atcaggctgt
      781 gggccgtcag ggaaggcgat cccacgatcc gtcaggtcgc cgtgggctgg accgacgacg
      841 cggtgcgagc cccggcgatc gtgaacctcg acggcgaccg ccggatcggg gtggccgggc
      901 cgggcccgtg ggccgacgcc gtggcccggg ctctggtcat ccaggtcacg gcccgcgccg
      961 ggcccaccgc ctgggccctc agcgttccgg ccgagccggc ctggcgcttc gcccgatggc
     1021 ttccccatca gcagggctcg ggagccgccc tgagcgtcgg ccacgacggt gccggtcccg
     1081 gcatcgaagt ggccgatcac cgggcgcagc tgaccgaccg ctgcgcggtc gtcctgacgt
     1141 ggagccccga cggcgcccgc ctgtgggccc caggccatgg tgacggacga ccggtccacc
     1201 ccttcctcgc ctcggtcgag ctcgccgagg actacgggcg ggcgtcggcc cggtggaccg
     1261 atcccgaccc ggcgggcgcc gccttgcccg atcggctggg gctggcgtcg ctcctgaccg
     1321 atgctgcggg ggcgagcgag gacgggcgcc gacgcctggc cgctccgttg ggcgtcggcg
     1381 tcgacggtgt cgtcacggtg gacctggtcg tcgacggccc ccacgcgctc gtggccggca
     1441 cgacgggtgc gggcaagagc gagctcctgc ggacctgggt cacggccctg gcccaccgcc
     1501 acccgccgac ggccgtggcc ttcgtcctcg tggactacaa gggcggcagc gccttcgacg
     1561 cctgcgcccg actgcctcac gtcaccgggg tcgtcaccga tctcgacgcc gggttgggcg
     1621 agcggctgct cgtcggcctg cgggccgaga tccgggaacg ggaggcgggc ctgcgcgacg
     1681 cgggggtggg cgacctccgg gacctcgtcg acggcccgcc gcggctggtg gtcgtggtgg
     1741 acgagttcgc cacgctggcc gccgagctcc cggacttcct cggcgcgctg gtcgacgtcg
     1801 cccgacgggg gcgcagcctc ggggtccacc tcgtgctggc cacccagcgc ccggccggcg
     1861 ccgtcaacga cgacatccgg gccaacaccg ctctccgcct ctgcctgcgc accctcgatg
     1921 ccgccgactc ccaggacgtg ctcggcgggc cggaggctgc gctgctccct gccgaccggc
     1981 cgggtcgggc ctggctccgt cagggccagg gcctgcgcct gctgcaggtc gccggcgggt
     2041 ccggcccgga ccggccgccg aacggtcccg gattggctgt acgccgcgtc ggcgacccgt
     2101 ggcccgacgg cgacgaggcc gggcccgtcg agctcgatcg gttggtcgac gaggtggcgg
     2161 gttcgtgggg ggatcgtccc cgacccacgc cggcctggcg gccgcccctc cccgaccacc
     2221 tgaggttgaa gcgccagatc ccggcgcggc gggaccccgc gcctctggtg ctcgggcaag
     2281 cggaccgccc cgaggaacgc acccaacctc cgctgacgtg gtccccgacc agcggccatc
     2341 tcgtcgtcct cgggggacga cggtcagggc gcacgaccgc ggtgcgcacg accgtgctcg
     2401 gcctggccgg gagccggtc
//
LOCUS       DASTFV010000015         4878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_53242, whole genome shotgun sequence.
ACCESSION   DASTFV010000015 DASTFV010000000
VERSION     DASTFV010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53242"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VFP02_00215"
     CDS             <1..443
                     /locus_tag="VFP02_00215"
                     /inference="COORDINATES: protein motif:HMM:NF027523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541473.1"
                     /translation="KPRRVTVHRYEVVPTDDPLVYRAEVECSSGTYIRTLAADLGVAL
                     GGGAHLTDLRRTRIGSFGEAEARPVEDVTLLPPKEGLRDYAQTTVDDEMAFLVGNGRV
                     LDPDQLGGVTGPGPWAVSDQSGRLLAVYERVSQHRVKPSLVLGH"
     gene            523..1479
                     /locus_tag="VFP02_00220"
     CDS             523..1479
                     /locus_tag="VFP02_00220"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET9541474.1"
                     /translation="MEVVRYPGPCPALPGGSVVTIGAYDGVHLGHRSIIDRVRKLAAD
                     RGIPSALITFDQHPARLVRPESAPLLLTDPEQKLELLGATGLDLLLVVRFDEERSQES
                     PEDFVREILVDCLGTKVVVVGEDFHFGHQRRGNVELLRTMGAEAGYAVEGVDLLGPDG
                     QVADDAHQVSSTAIRTALALGDVERAAELLGRHHEVRGVVVRGDGRGGPELGCPTANV
                     AVPDDVLLPADGIYAGWYTRPDGSRHASAISLGRRPTFYETAHASLLEAHLLDFSGDL
                     YDERARVAFVARLRGEVKFDGVEALIEQIGRDCEQARQILLG"
     gene            1506..2612
                     /locus_tag="VFP02_00225"
     CDS             1506..2612
                     /locus_tag="VFP02_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9541475.1"
                     /translation="MVAVPDLTDPLVGPEGPGALAAVEGRRPNPRLAQSTVTLPDGHQ
                     VGVSVTGQGIPLVVVHGFSAEGILYAQTLSRLVGMGYRVIAVDTAGHGGTFGLPAGGG
                     DLAEYSALIGRVMDHLGIRRAVLAGHSMGGRLVVELAATEPDRAIALVLLDAIVGESW
                     DRIQQWSRWVPGVLGVIGATLMVDAATTLPVFRDPKQAVKLVRLGLPTAVAHARRPWR
                     LMAPAISILRSSPSCALLEEVARQGISTFVLHGDRDYAVPLSTAKSAAHLANGQLVVI
                     RGASHAWVLKDPETLPAIVYELLGEGLGAAQRRALLHAGLLPGEVSLTDVEEAFYERG
                     ALVQRLAPAELTTQGGEPAPPTRRPRFSWTVTTP"
     gene            2906..3184
                     /gene="rpsO"
                     /locus_tag="VFP02_00230"
     CDS             2906..3184
                     /gene="rpsO"
                     /locus_tag="VFP02_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET9541476.1"
                     /translation="MSRPPTNLPPKSATMAEHGKHDTDTGSPEVQVALLTDRINHLTE
                     HLRVHKKDHHSRRGLLMLVGRRRRLLDYLAKNDVERYRALIAKLGLRR"
     gene            3374..>4878
                     /locus_tag="VFP02_00235"
     CDS             3374..>4878
                     /locus_tag="VFP02_00235"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET9541477.1"
                     /translation="MAENHSVSRPVTGTDKTLSFESGKLAGLADGAVTVRMGDTMVLV
                     TATASRSVREGADFFPLTVDIEERFYAAGKIPGSFFRREGKSSDQAILTCRLIDRPLR
                     PSFPEGFRNEVHVVGTILGADQENPHDILAINAASAALCISPIPFDGPLGAVRLAFTP
                     EGTWVAYPTWEEGKDSTFEMVVAGRLLDDGDVAVMMVEAGGGEHAWQLYEAGAPKVTE
                     EIVAAGLEASKTHIKDAIELQQELIDALGGKPEVFEFTVASDYSDEVFAAVEAAATDR
                     VASTYQIADKAERTGVEDEMKAAVVAELLPQFEDVSGADKQIKAAIRSLTKKVVRKRI
                     VEDGIRIDGRGTRDVRPLSSEVHLVPTAHGSGLFQRGETQVLNVTTLGMPRMEQMLDS
                     LGGDTSKRYMHHYNFPPFSTGETGFMRGPKRREIGHGLLAERALLPVIPPSEEFSYTL
                     RTVSDVLSSNGSTSMGSVCASTLSLMDAGVPIKAPVAGIAMGLVYAEGKYTA"
BASE COUNT          701 a         1845 c         1605 g          727 t
ORIGIN      
        1 gcaagccccg ccgggtgacc gtccaccggt acgaggtggt gcccaccgac gacccgctgg
       61 tctaccgggc cgaggtcgag tgctcgtcgg gcacctacat ccgcaccctg gccgccgacc
      121 tgggcgtcgc cctcggtggc ggcgcccacc tgacggacct gcggcgcacg cggatcggct
      181 ccttcggtga ggccgaggcc cgccccgtcg aagatgtgac cctgctccca cccaaggagg
      241 gcctgcggga ctacgcgcag accaccgtcg acgacgagat ggccttcctg gtgggcaacg
      301 gccgggtcct cgaccccgac cagctcggcg gtgtcaccgg ccccggcccc tgggccgtca
      361 gcgaccagtc cggccggctg ttggccgtct acgaacgcgt ctcccagcac cgcgtcaagc
      421 ccagcctcgt cctcggccac tgacccgtct cgttctggcg cctgatttcg gggtcagggc
      481 cccagaatcg ggcgccagaa ggaggggggt accctcatcg gcgtggaggt ggtgcggtac
      541 ccggggccct gcccggcgct cccgggtggc tcggtggtca ccatcggggc ttacgacggt
      601 gtccatctcg ggcaccggtc catcatcgac cgggtgcgga agctggccgc tgaccggggg
      661 atcccgtccg cgctgatcac cttcgaccag caccccgccc gcctggtccg ccccgagagc
      721 gccccgctcc tgctgaccga ccccgagcag aagctggagc tgctgggggc caccggcctc
      781 gacctgctcc tcgtcgtccg cttcgacgag gagcgttccc aggagtcgcc ggaggacttc
      841 gtccgcgaga tcctggtcga ctgcctcggg accaaggtgg tcgtggtcgg ggaggacttc
      901 cacttcggtc accaacgtcg gggaaacgtc gagctcctgc gcaccatggg cgctgaggcc
      961 ggctatgcgg tcgaaggggt cgacctgctc ggtcctgacg gccaggtcgc cgacgacgcc
     1021 caccaggtct cgtcgaccgc catccgcacc gccctggccc tgggcgacgt cgagcgggcg
     1081 gcggagctgc tcggccgtca tcacgaggtc cggggcgtgg tcgtccgcgg cgacggtcgg
     1141 ggcgggcccg agctcgggtg cccgacggcg aacgtggcgg tgcccgacga cgtgctcctg
     1201 cccgccgacg gcatctacgc cggctggtac acccgccccg atggctcccg gcacgcgtcg
     1261 gcgatctcgc tgggccggcg cccgacgttc tacgagaccg cccatgcctc gctgctggag
     1321 gcccatctgc tggacttctc gggtgacctc tatgacgagc gggcccgggt cgccttcgtg
     1381 gcccgactgc ggggcgaggt gaagttcgac ggggtcgaag ccctcatcga gcagatcggt
     1441 cgggactgcg agcaggcccg tcagatcctg ttgggctgag gacgacgatc atcgccgaca
     1501 aacggttggt cgccgttccc gacctgaccg atcccctggt gggcccggaa gggcccgggg
     1561 cgcttgccgc ggtcgaaggt cgtcggccca acccccggtt ggcccagtcc acggtgaccc
     1621 tgcccgacgg ccaccaggtc ggcgtgtcgg tgaccggcca gggcatcccc ctggtggtgg
     1681 tccacggctt ctcggccgaa gggatcctct acgcccagac cctgtcccgc ctggtgggca
     1741 tgggctaccg ggtcatcgcc gtcgacaccg ccggccacgg cgggaccttc ggcctgcctg
     1801 ccggcggtgg tgacctcgcc gagtactcgg cgctgatcgg ccgggtcatg gaccacttgg
     1861 gcatccgccg ggccgtgctg gcggggcact cgatgggtgg tcgcctggtc gtggaactgg
     1921 ccgccacgga gccggaccgg gccattgccc tggtgctgct cgacgccatc gtcggtgaga
     1981 gctgggaccg catccaacag tggtcccgct gggtcccggg cgtcctgggc gtcatcggcg
     2041 ccacgctcat ggtggacgcc gccaccaccc ttccggtgtt ccgggacccg aagcaggcgg
     2101 tcaagctcgt tcgcctcggc ctgcccaccg ccgtcgccca cgcccgtcgc ccctggcggc
     2161 tcatggcccc cgccatctcg atcctgcgtt cgtcaccgag ctgcgcgctg ctcgaggagg
     2221 tcgcccgcca gggcatctcc accttcgtgc tccacggtga ccgggactac gccgtgcccc
     2281 tgtccacggc caagtcggcc gcccacctgg ccaacggtca gctcgtggtc atccgcgggg
     2341 cgtcgcacgc ctgggtgctg aaggatcccg agaccctgcc cgccatcgtc tacgagctac
     2401 tcggggaggg tctcggggcc gcccagcgtc gggccctgct ccacgccggc ctcctgcccg
     2461 gcgaggtctc cctgaccgac gtcgaagagg cgttctacga gcgcggcgcc ctcgtccagc
     2521 gcctcgcccc cgccgagctc accacccagg gcggcgagcc cgcacccccc acccgccggc
     2581 cccgtttcag ctggacggtc acaacgccat gacacctgcg tggcttcact tcgttcagca
     2641 acgccggctc cgccgaagct ccgaggcctc ggcgctgctc cactccgttg cgcagcctca
     2701 ccgcccggct ggtcccgctc cagtggttag gcgccgttcg ctggctggtc agctgctcca
     2761 ctccgttgcg cagcctcacc acccgcctgg tcccgcccca gtgccggccg gggtctttcc
     2821 cgaggttcgc tggtagtctg cctccagaaa ccaggcgcat ccccatcctc cgcgccccag
     2881 acgaagagaa gacgaagaga cgcagatgtc gcgaccgccc accaacctcc ccccgaagtc
     2941 ggccacgatg gccgagcacg ggaagcacga caccgacacg ggttctcccg aagtccaggt
     3001 ggcgctgctc accgatcgga tcaatcacct gaccgagcac ctgcgggtgc acaagaaaga
     3061 tcaccacagc cgccgcggcc tgctcatgct ggtcggccgc cgacgccggc tgctcgacta
     3121 cctcgccaag aacgacgtcg agcgctaccg cgccctcatc gccaagctcg gcctccggcg
     3181 ttaacgatct ccctccggct ggccacctcg gtggccggcc cccactgaaa gtccaagaca
     3241 gcgcagcggt tcagctgcca gtcgccgact ccccggccca gaggtgatgc cggaccgggt
     3301 ggccgacaac tgggaactgg acggctgctc cacacagcgg gacccgaagg cgggtcccag
     3361 aagagagcat ccaatggcgg agaatcactc cgtatcccgt cccgtcaccg ggactgacaa
     3421 gaccctgtcc ttcgaatccg gcaagctggc cggcctcgcc gacggcgccg tgaccgtccg
     3481 catgggcgac acgatggtgc tggtcacggc caccgcctcg cgttcggtgc gggagggggc
     3541 ggacttcttc cccctcaccg tcgacatcga ggagcgcttc tacgccgcgg gcaagatccc
     3601 cggctcgttc ttccgtcggg agggcaagtc cagcgaccag gccatcctga cctgccgtct
     3661 gatcgaccgc ccgctgcgcc cctcgttccc cgagggcttc cgcaacgagg tccacgtggt
     3721 cggcaccatc ctgggcgccg accaggagaa cccccacgac atactggcca tcaacgccgc
     3781 ctccgcggcg ctgtgcatct cgcccatccc cttcgacggg ccgctgggtg cggtgcgcct
     3841 ggccttcacc cccgagggca cctgggtcgc ctacccgacc tgggaagagg gcaaggactc
     3901 caccttcgag atggtcgtgg ccggtcgtct gctggacgac ggcgacgtcg ccgtcatgat
     3961 ggtcgaggcc ggcggcggtg agcacgcctg gcagctctac gaggcgggcg cccccaaggt
     4021 caccgaggag atcgtggccg cgggcctcga ggcctccaag acccacatca aggacgccat
     4081 cgagctccag caggagctca tcgacgccct cggcggcaag cccgaggtct tcgagttcac
     4141 ggtcgccagc gactacagcg acgaggtctt cgccgccgtc gaggccgccg ccaccgatcg
     4201 ggtggccagc acctatcaga tcgcggacaa ggccgagcgc accggcgtcg aggacgagat
     4261 gaaggccgcg gtcgtcgccg agctgctccc gcagttcgag gacgtgtccg gcgccgacaa
     4321 gcagatcaag gccgccatcc ggtcgctgac caagaaggtg gtgcgcaagc gcatcgtcga
     4381 ggacggcatc cgcatcgacg gtcgcggcac ccgggacgtg cggcccctgt cctccgaggt
     4441 ccacctggtc cccacggcgc acggctccgg cctcttccag cggggcgaga cccaggtcct
     4501 caacgtgacc acgctgggca tgccccgcat ggagcagatg ctggacagcc tcggtggcga
     4561 cacctccaag cgctacatgc accactacaa cttcccgccc ttctccaccg gcgagaccgg
     4621 cttcatgcga ggcccgaagc gccgcgagat cggccacggc ctcctggccg agcgggccct
     4681 gctgcccgtc atcccgccgt cggaggagtt cagctacacg ctgcgcaccg tgtccgatgt
     4741 gctgagctcc aatggctcca cctcgatggg ctcggtctgt gcctcgaccc tgtcgctgat
     4801 ggacgccggg gtgccgatca aggcccccgt ggccggcatc gccatgggcc tcgtctacgc
     4861 cgagggcaag tacacggc
//
LOCUS       DASTFV010000016         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_62521, whole genome shotgun sequence.
ACCESSION   DASTFV010000016 DASTFV010000000
VERSION     DASTFV010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62521"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFP02_00240"
     CDS             <1..272
                     /locus_tag="VFP02_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541478.1"
                     /translation="PVSARALAEAIADQVDESSDGGRMFEIAGPQEELLPDLARLLIE
                     AEGRPVEVEAVVDPVDGERHEQGALLPGPDARLVGPTYTDWLAAG"
     gene            347..1252
                     /locus_tag="VFP02_00245"
     CDS             347..1252
                     /locus_tag="VFP02_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9541479.1"
                     /translation="MDIPILPSAPDWFRDALAVPFTDETVEVEGVPIHSLAWGRPGTR
                     GLVFVHGGGANAHWWTHVAARFVGDFRVVAIDLSGHGDSGHRETYALDTWTDEVVAVA
                     EAGGIEGAPVVIGHSMGGFVSIATAARHPDRCAGVVVCDSPVTEPDPEIASFHLKEAF
                     GRPRTYDTVEDALARYRTVPPQDHYLDFVMDHVGRQSLKPVEGGYQWKFDRRIFEQFS
                     EGMRSIALPYLPEVGCRLALLRSENGLVTEDIGASMYDKLGRVTPVIEIPEAGHHAML
                     DQPLILLTALRAFLADWDHSEPLTR"
     gene            1349..2107
                     /locus_tag="VFP02_00250"
     CDS             1349..2107
                     /locus_tag="VFP02_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9541480.1"
                     /translation="MEIEGKGAVIVGGASGMARASAEQLHAKGAKIAILDLPTSKGEE
                     VAAALDGTFHPVDVTDYEGVEIVLAEAVEVLGGLHIAVNTAGGGAAARTLGREGPLPL
                     EVFRRTIDLNLVATFNLNRLQAQHMAENEPEDEERGVIIDTASIAAFEGQIGQVAYTA
                     AKAGIAGMMLTMARDLGSLGIRAVAIAPSLFSTGLTEGIPDEFADVLTKDAAFPKRMG
                     RPDEYGRLVVAIVETAMLNGGCIRLDAGQRFAPK"
BASE COUNT          330 a          781 c          773 g          317 t
ORIGIN      
        1 agccagtcag cgccagggcg ctggccgagg cgatcgccga ccaggtcgac gaatcgtcgg
       61 acggcgggcg gatgttcgag atcgccggcc cgcaggagga gctgctgccc gacctcgccc
      121 ggctgctgat cgaggccgag ggtcggccgg tcgaggtcga ggccgtggtc gaccccgtcg
      181 acggggagcg ccacgagcag ggtgccctcc tgcccgggcc cgacgcccgg ttggtggggc
      241 cgacctacac cgactggctc gccgccggct gatcttgcgg cagatccggc cccttcgggg
      301 gcgggatcgg ccgtcggttc gggtgggcgg gcaggatgga cggttgatgg acatcccgat
      361 cctcccctcg gcccccgact ggttccgtga cgccctggcc gtgccgttca ccgacgagac
      421 ggtcgaggtc gagggcgtcc cgatccactc gttggcctgg ggtcggccgg gcacccgcgg
      481 gctggtgttc gtgcacgggg gtggggccaa tgcccactgg tggacgcacg tggcggcccg
      541 cttcgtcggg gacttccggg tggtggccat cgacctctcc ggtcatggtg acagcgggca
      601 ccgggagacc tacgcgctgg acacctggac cgacgaggtc gtggcggtgg cagaggccgg
      661 cgggatcgaa ggcgcgccag tcgtgatcgg ccactccatg ggtggcttcg tcagcatcgc
      721 caccgcggcc cgccatcccg accgttgtgc aggggtcgtc gtctgtgact cgccggtcac
      781 cgagcccgat cccgagatcg cctcgttcca cctcaaggag gccttcggtc ggccccggac
      841 ctacgacacc gtcgaggacg ccctcgcccg ctaccgcacg gtcccacctc aggaccacta
      901 cctggacttc gtgatggacc acgtcgggcg gcagtcgctg aagccggtcg agggtggcta
      961 ccagtggaag ttcgaccgac gcatcttcga gcagttcagc gagggcatgc gctccatcgc
     1021 cctgccgtac ctgcccgagg tcggctgccg cctggccctc ctgcgctccg agaacggcct
     1081 cgtcaccgag gacatcggtg cgtccatgta cgacaagctc ggccgggtca cgccggtcat
     1141 cgagatcccc gaagccggcc accacgccat gctcgaccag cccctgatcc tgctgaccgc
     1201 cctccgggcc ttcctcgccg actgggacca ctccgagccg ctgacccgct aggcgaacgc
     1261 ccgacggggg aagcggccgc gtttcgacgg gcggaccgcc tcctcagtac cgtccccggg
     1321 ccacaggatc cgagaaggga caaaccacat ggagatcgag ggcaagggtg cggtcatcgt
     1381 cggcggggcg tcgggcatgg cccgggcctc ggcggagcag ctccacgcca agggggccaa
     1441 gatcgccatc ctcgacctgc ccacctcgaa gggcgaggag gtggccgccg ccctggacgg
     1501 aacgttccat ccggtggatg tcaccgacta cgaaggcgtc gagatcgtgc tggccgaggc
     1561 cgtcgaggtg ctcggcggcc tgcacatcgc ggtgaacacg gccggcggtg gggccgccgc
     1621 ccggacgttg ggccgggagg gcccgctccc gctggaggtg ttccgccgga ccatcgacct
     1681 gaacctggtc gccaccttca acctgaaccg gctgcaggcc cagcacatgg ccgagaacga
     1741 gcccgaagac gaggagcggg gcgtcatcat cgacaccgca tccattgccg ccttcgaagg
     1801 ccagatcggc caggtcgcct acaccgcagc caaggcgggc atcgcgggga tgatgctgac
     1861 catggcccgg gacctgggct cgctcggcat ccgggccgtg gccatcgccc ccagcctgtt
     1921 cagcacgggc ctcaccgagg gcatccccga cgagttcgcc gacgtgttga ccaaggacgc
     1981 cgccttcccg aagcggatgg gtcgtcccga cgagtacggc cgcctggtgg tcgccatcgt
     2041 cgagacggcg atgctcaacg gtggctgcat ccgcctcgac gccgggcagc gcttcgctcc
     2101 gaaataggca gactgtcgtc atgtccatcg cgctgaccga cgatcacaag gccctggccg
     2161 acaccgcctc cgagctgctg gcgaagcggg atgcccgcgg g
//
LOCUS       DASTFV010000017         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_66203, whole genome shotgun sequence.
ACCESSION   DASTFV010000017 DASTFV010000000
VERSION     DASTFV010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66203"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..1497
                     /gene="pyk"
                     /locus_tag="VFP02_00255"
     CDS             55..1497
                     /gene="pyk"
                     /locus_tag="VFP02_00255"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET9541481.1"
                     /translation="MMSERTKVVATLGPASSTPEILGGMVEAGLDVVRLNFSHGEKAD
                     HQARFELVRSVATERNRNLAILVDLQGPKIRVGLVDDDGVKLDRGQEVVLVAGVDRAE
                     APEIPVVYPALADDVRPGDQILLDDGAIGLRVVDVDGKRVRSRVERGGVVKSRKGVNL
                     PGVAVSAASLTRKDRADVVTAVEAGADYLALSFVRRPDDVAEAKQAIAEAGGDIPVVA
                     KLERPEAIDRLDEILAVADAVMVARGDLGVELAVEQVPPIQKHIIARANSLGVPVITA
                     TQMLESMVASPRPTRAEASDVANAIFDGTDAVMLSQETAIGQYPVEAVATMARIAREA
                     EATPYLAAPPPPAGGALDVPATVCRAAVQIANDLGCTAIVAFTETGASARYVSRFRPR
                     TPILGLTTSEAARRRMALYWGVETATPLGVGTQVRSMIDDADVRILREGLLKRGDLVV
                     VVAGSPGGRGGTNRVLVHRVGEADLAAREA"
     gene            1668..2159
                     /locus_tag="VFP02_00260"
     CDS             1668..2159
                     /locus_tag="VFP02_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541482.1"
                     /translation="MKQREVGEAYEASTSALEAAKGQRLSLRRAIAELEEALAITDDP
                     DGIGGLDSLPARLAPALERLQEVFAVHVEVTEAPGGLYHEILENSPRLANRVTRFRRE
                     HAEIADGIRRGIAEAATATDPDAVQALRDHAVRLFADLVRHGKRGLDLVYEAYHVDIG
                     GES"
     gene            complement(2167..>2549)
                     /locus_tag="VFP02_00265"
     CDS             complement(2167..>2549)
                     /locus_tag="VFP02_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541483.1"
                     /translation="DRLLAATTCRVHRRVPAGLTMDDAYAACDAVVFPSTWEGFGAPL
                     IEASLHRRALAIGDYPVADELAAFGFRWFPADDPTPLKAFLDHPDPSLLDGNHDIAAR
                     RFSLDALTTQLATLLDNPGWRRLG"
BASE COUNT          368 a          919 c          953 g          309 t
ORIGIN      
        1 cgtcaccggc caaccttcgc agcgtggccc cggtcgtgcc acactgccga cacgatgatg
       61 tccgagcgta ccaaggtcgt ggccaccctc ggcccggcct cgagcacccc cgagatcctc
      121 ggtggcatgg tcgaggccgg cctcgacgtc gtgcggctca acttctccca cggcgagaag
      181 gccgaccacc aggcccggtt cgagctggtc aggtcggtgg ccaccgagcg caaccgcaac
      241 ctggccatcc tggtcgacct ccaggggccc aagatccgcg tcgggctggt cgacgacgac
      301 ggggtcaagc tcgaccgcgg ccaggaggtc gtgctggtcg ccggggtcga ccgggccgag
      361 gcgcccgaga tcccggtcgt ctacccggcc ctggccgacg atgtccggcc gggcgaccag
      421 atcctgctgg acgacggggc catcggcctg cgggtcgtgg acgtcgacgg gaagcgggtc
      481 cgcagccggg tggagcgggg cggcgtcgtc aagtcccgca agggcgtgaa cctgcccggg
      541 gtggctgttt cggcggcctc gctgaccagg aaggaccggg ccgacgtggt caccgcggtc
      601 gaggccgggg ccgactacct ggccctgtcg ttcgtgcgca ggcccgacga cgtggccgag
      661 gccaagcagg ccatcgccga ggccggcggc gacatcccgg tggtggccaa gctggagcgg
      721 ccggaggcga tcgaccgcct tgacgagatc ctggcggtcg ccgacgccgt catggtcgcc
      781 cggggcgacc tcggggtcga gttggccgtc gagcaggtcc cgccgatcca gaagcacatc
      841 atcgccaggg ccaactcgct cggggtgccg gtgatcaccg ccacccagat gctcgagtcg
      901 atggtggcca gtccccgccc gacccgtgcc gaggccagcg acgtggccaa cgcgatcttc
      961 gacggcaccg acgcggtgat gctctcccag gagaccgcca tcggccagta cccggtcgag
     1021 gcggtcgcca ccatggcccg gatcgcccgg gaggccgagg cgaccccgta cctggcggcc
     1081 ccgccgccgc cggccggcgg cgcgctggac gtgcccgcca ccgtctgccg ggcggccgtg
     1141 cagatcgcca acgacctggg atgcacggcg atcgtggcct tcaccgagac gggggcgagc
     1201 gcccgctacg tctcccggtt ccggccgcgg accccgatcc tcggcctgac cacctccgag
     1261 gcggcccgcc gccggatggc cctctactgg ggggtggaga ccgccacccc gctgggcgtc
     1321 ggcacccagg tgcggagcat gatcgacgac gccgacgtcc ggatcctccg cgagggcctg
     1381 ctcaagcggg gcgacctggt cgtggtcgtg gccggcagcc ccggcgggcg gggcgggacc
     1441 aaccgggtgc tcgtccaccg ggtcggcgag gccgacctgg ccgcccgcga agcttgaggc
     1501 acaaagggca tggagtcggc cttcggccga caatcatcac actccgcccg gcagagccgg
     1561 tcggcaaagt tctacggtga caacggatcc gctttccgcc acacttccgt gaccacccgt
     1621 gaccaccgtc cgggggacac ggcggggcgc gtgggaggag ccggcgtatg aagcaacgtg
     1681 aggtcggcga ggcgtacgag gcctccacca gcgccctgga ggcggccaag gggcagcggc
     1741 tcagcctccg gcgggccatc gccgagctgg aggaggccct ggccatcacc gacgaccccg
     1801 acggcatcgg cggcctcgac agcctcccgg ccaggctggc cccggccctg gagcgcctcc
     1861 aggaggtctt cgccgtccac gtcgaggtca ccgaggcacc gggcggcctc taccacgaga
     1921 tcctcgagaa ctccccccgc ctggccaatc gcgtcacccg gttccgccgc gagcacgccg
     1981 agatcgccga cggcatccgc cgcggcatcg ccgaggccgc cacggccacc gaccccgacg
     2041 cggtccaggc cctccgcgac cacgccgtcc gcctgttcgc cgacctggtc cgccacggca
     2101 agcgcggcct cgacctggtc tacgaggcct accacgtcga catcggcggc gaatcctagc
     2161 caccgcctag cccagccgcc gccagcccgg gttgtcgagc agggtggcga gctgggtggt
     2221 gagggcgtcg agggagaagc ggcgggcggc gatgtcgtgg ttgccgtcca gcagggacgg
     2281 gtcggggtgg tcgaggaagg ccttgagggg ggttgggtcg tcggcgggga accagcggaa
     2341 gccgaaggcg gccagctcgt cggcgaccgg gtagtcgccg atggccaggg cacggcggtg
     2401 cagggaggcc tcgatcaggg gggcgccgaa gccctcccag gtcgagggga agaccacggc
     2461 gtcgcaggcg gcgtaggcgt cgtccatggt caggccggcg gggacgcggc ggtggacccg
     2521 gcaggtggtg gcggcgagca ggcggtcga
//
LOCUS       DASTFV010000018         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_68852, whole genome shotgun sequence.
ACCESSION   DASTFV010000018 DASTFV010000000
VERSION     DASTFV010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68852"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFP02_00270"
     CDS             <1..183
                     /locus_tag="VFP02_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541484.1"
                     /translation="RIRPPTESNYETVGVRGVEARYTPGADRLEWVEDGLVLSLRGTG
                     LTRAELVSVADQLEPL"
     gene            180..1400
                     /locus_tag="VFP02_00275"
     CDS             180..1400
                     /locus_tag="VFP02_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET9541485.1"
                     /translation="MRRAVLVGLCLLVGLAVGLGGALALGDDTDPTSTDSAAPSTTVS
                     STTARPPATTGPPPTTAPGPPPPSVLLAWTAGGLPSGFGDALGVLPAVVDEAVVRGDL
                     RGLIETDDGRGNTVDRVEPGWEIPFDVLALDPAQFGSFVTAPVRRVLRRLGPDEVVLS
                     ETSAGLRRLEVGGRIHLESGPSLRVVGIVPDVSVAGAEAVVTPSTGAAIGVTTERYVL
                     LHHRGRRAPVDDAIRMLAGEPVRTRAGGETPFLRHADGVLPQALIKAQFGEFSYRDTG
                     PGPVTLDPAWVAANIVQTDLPVLGPTTCHRTLLPALQGALRDLVAANLAHLVASFEGC
                     YNARTITGSTQLSRHAWGAAVDLNFSSNPTGVGSMQDARLVDIFERWGFGSGDFWLVP
                     DSGHFEYIAPPSPG"
     gene            complement(1529..>2044)
                     /locus_tag="VFP02_00280"
     CDS             complement(1529..>2044)
                     /locus_tag="VFP02_00280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9541486.1"
                     /translation="GGGRLSKVENKSDDLFGHGFAVGFYGPVWAMQAAFPVMRDQGRG
                     RIINLCSLNGVNAHIGTAEYNSAKEALRALTRTAAREWAPHGITANVICPGAKTAASR
                     AVFAEHPELEQMADAANPMGRLGDPDTDIAPVAVFLAADDSRYLTGNTLFVDGGSHIN
                     GAPWAPELPDD"
BASE COUNT          248 a          730 c          771 g          295 t
ORIGIN      
        1 cggatccgcc cgccgaccga atcgaactac gagacggtcg gggtccgcgg ggtcgaggcc
       61 cgctacaccc ccggcgctga tcggctggag tgggtggagg acggcctcgt gctgtcactg
      121 cggggcacgg gactgacccg ggccgagctc gtgtccgtgg ccgaccagct ggagcccctg
      181 tgaggcgggc cgtcctcgtc gggctctgcc tgctggtcgg gctggccgtg ggcctgggcg
      241 gagcgctggc cctgggcgac gacaccgatc cgacatcgac cgactccgcc gccccgtcga
      301 ccaccgtctc gtcgacgacg gctcggccgc cggcgaccac tggcccgccg ccgacgaccg
      361 cacccggccc gcccccaccg agtgtcctgc tggcctggac cgccggcggc ctgccgagcg
      421 ggtttggcga cgccctgggc gtcctgcccg cggtggtgga cgaagccgtg gttcggggcg
      481 acctgcgagg cctgatcgag accgacgacg gccgggggaa cacggtggac cgggtcgagc
      541 cgggctggga gatcccgttc gacgtgctcg ccctggaccc ggcgcagttc ggttccttcg
      601 tgaccgcgcc ggtgcggcgg gtcctgcgcc gactggggcc ggacgaagtc gtcctcagtg
      661 agacgtcggc gggactgcgg cgcctcgagg tcggcggacg gatccacctg gagtccggcc
      721 cgagcttgcg ggtcgtcggg atcgtgcccg acgtgagcgt ggccggcgcc gaggccgtgg
      781 tcacgccgtc gacgggcgcc gccatcggcg tgaccaccga gcgctacgtg ctgctgcacc
      841 accggggccg tcgggccccg gtcgacgacg cgatccggat gttggcgggc gagccggtgc
      901 gcacccgggc gggcggggag accccgttcc tccggcacgc cgacggcgtc ctcccccagg
      961 ccctcatcaa ggcccagttc ggtgagttct cgtaccggga caccggcccg ggcccggtca
     1021 ccctcgaccc ggcgtgggtg gcggccaaca tcgtccagac cgacctcccc gtcctgggcc
     1081 cgaccacgtg tcaccggaca ctgctgcccg cgctgcaggg ggcgctccgc gacctggtgg
     1141 cggccaacct ggcccacctg gtcgccagct tcgaggggtg ctacaacgcc cgcacgatca
     1201 ccgggagcac ccagctgtcc cgtcatgcct ggggagcggc ggtcgacctg aacttctcct
     1261 ccaacccgac cggtgtgggg tcgatgcagg acgcccgtct ggtcgacatc ttcgagcggt
     1321 ggggcttcgg ctccggcgac ttctggctgg tgcccgacag cggccacttc gagtacatcg
     1381 ccccgccctc gccggggtga ggccggggcg cgacggacac ggcgcgtcgg gtgcggccgg
     1441 tgaggggggt gccgtcccca tgcctgacct gaccccgagt cagcgcctgt atgacggcgg
     1501 tcacaacggg tcccgtccgg ggaagccctc aatcgtcggg cagctccggg gcccacgggg
     1561 cgccgttgat gtggctgccg ccgtcgacga agagggtgtt gccggtgagg tagcggctgt
     1621 catccgcggc caggaagacg gcgaccgggg cgatgtcggt gtcgggatcg ccgaggcggc
     1681 ccatggggtt ggcggcgtcg gccatctgct ccagctcggg gtgctcggcg aacacggctc
     1741 gggacgccgc cgtcttggcg cccgggcaga tgacgttggc ggtgatgcca tggggcgccc
     1801 attcgcgggc ggccgtccgg gtcagcgccc ggagcgcctc cttggccgag ttgtactcgg
     1861 ccgtgccgat gtgggcgttg accccgttga gggagcagag gttgatgatc cggccccgac
     1921 cctggtcccg catcaccggg aaggcggcct gcatggccca cacggggccg tagaacccga
     1981 cggcgaagcc gtgcccgaag aggtcgtcgc tcttgttctc gaccttgctc aggcgaccgc
     2041 cacc
//
LOCUS       DASTFV010000019         2583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_71633, whole genome shotgun sequence.
ACCESSION   DASTFV010000019 DASTFV010000000
VERSION     DASTFV010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71633"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFP02_00285"
     CDS             complement(<1..232)
                     /locus_tag="VFP02_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541487.1"
                     /translation="MRAPVQTSSRPRRRALGLAVALLSLAGVAVTGCSDDGPSSPAIS
                     GNSPSDVSVPTVPTTTETTVPGTTAGTDTTTTT"
     gene            280..1569
                     /locus_tag="VFP02_00290"
     CDS             280..1569
                     /locus_tag="VFP02_00290"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9541488.1"
                     /translation="MPWLFLIAALIGFWFTLNAFRPVRRNTLFFGQSFFAGWLTTELA
                     GHHLVWQFAMTVVFVATGALDAWPGWVALAVTLVSWTGLVVLLVQGHRAARQVDDALG
                     HLLAGDPPPGIPWSYLVLPFRMKRKGVDRDRDIEFARAGGRVLRLDVYRPSAPPADGK
                     RRPAVLQIHGGGWVVGDKREQGINLLGHLAVNGWVGFNANYRLSPAATWPDHLVDVKR
                     AVAWIREHADEYDVDPDFIAVTGGSAGGHLTALVALTANDPRYQPGFEDADTSVQAAA
                     PFYGVYDWTNRQGMAHPDMLRRFLEPWVVKAFFADEPEKFHDASPIDQVGADAPPFFV
                     LHGDRDTLAPVEDARLFVDRLREVSDTPVIYAELAGAHHAFDLFVSTRAKPVLESVER
                     FFAAVYEQAQGTGAPVPEADIEGGGHGDEAEVVSASS"
     gene            1613..1873
                     /locus_tag="VFP02_00295"
     CDS             1613..1873
                     /locus_tag="VFP02_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541489.1"
                     /translation="MVLLLGSTIGVLVVGAAAGARHRARHRWVSPYTDLATAMRAVTR
                     TEASEFRPTGLVALRPAPMPPRADIVDLGSVRALRHQESAAV"
     gene            1978..>2583
                     /locus_tag="VFP02_00300"
     CDS             1978..>2583
                     /locus_tag="VFP02_00300"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9541490.1"
                     /translation="MRGALMRNAGDDALEVVDGLEIGEPGPGQVKVKIVATGVCHSDL
                     SVLNGTIPQPPPVILGHEGAGIITSVGEGVPGLAEGDHVIIAWSPPCGTCADCVDRKS
                     PHLCQAFLFNMAIPKYTLDGVDVYGMAGAGTWAEETIMPWQGAIRIDDDVPFEVASLI
                     GCAVMTGVGAAINTAKVEPGSTVVVFGCGGVGISVIQGARVC"
BASE COUNT          346 a          928 c          909 g          400 t
ORIGIN      
        1 cggtcgtggt ggtggtgtcc gtccccgcgg tcgtgccggg caccgtggtc tcggtggtcg
       61 tcggcacggt cggcacggac acgtcactgg ggctgttgcc gctgatcgcg ggcgaggacg
      121 gaccgtcgtc gctgcagccc gtgacggcta cgccggcgag ggagaggagg gccacggcga
      181 ggcccaaggc ccggcggcgg ggccgcgaag aggtctgaac gggggcgcgc acgtccgtgg
      241 gactaccact tcgggcgggg gtgacgccag actggggacg tgccttggct cttcctgatc
      301 gccgcgctca tcggtttctg gttcaccctg aacgccttcc gcccggtgcg gcgcaacacc
      361 ctcttcttcg gccagagctt cttcgcgggc tggctcacca ccgagctggc cggacatcac
      421 ctggtctggc agttcgcgat gaccgtggtg ttcgtggcca ccggcgcgct cgacgcctgg
      481 cccggctggg tcgccctggc cgtgaccctc gtgtcctgga cggggctggt ggtcctgctg
      541 gtccagggtc accgggcggc ccggcaggtc gacgacgcgc tcggtcacct gctggccggc
      601 gatccacctc ccggcatccc ctggagctac ctcgtcctgc cgttccggat gaagcgcaag
      661 ggcgtggacc gcgaccgtga catcgagttc gcccgggccg gcggccgggt cctgcgcctc
      721 gacgtctatc gcccgtcagc gccgccggcg gacgggaagc gacggccggc ggtcctccag
      781 atccacggcg ggggctgggt cgtcggcgac aagcgggagc agggcatcaa cctgctcggg
      841 cacctggccg tcaacggctg ggtggggttc aacgccaact accgcctgag ccccgcggcc
      901 acctggcccg accacctcgt ggacgtgaag cgggcggtgg cctggatccg ggagcacgcc
      961 gacgagtacg acgtcgaccc cgacttcatc gccgtcaccg gcggttcggc gggagggcac
     1021 ctcaccgccc tggtcgccct cacggccaat gacccccgct accaacccgg cttcgaggac
     1081 gcggacacgt cggtgcaggc cgcggcgccg ttctacggcg tctacgactg gaccaatcgg
     1141 caggggatgg cccaccccga catgcttcgt cgcttcctcg agccctgggt ggtgaaggcc
     1201 ttcttcgccg acgagccgga gaagttccac gacgcctcac ccatcgacca ggtgggcgcc
     1261 gatgccccgc cgttcttcgt cctccacggt gaccgggaca ccctggcgcc ggtcgaggac
     1321 gcccgcctct tcgtcgaccg gttgcgcgag gtcagcgaca ccccggtgat ctacgccgag
     1381 ctggcggggg cccatcacgc cttcgacctc ttcgtgtcca cgcgggccaa gccggtgctc
     1441 gaatcggtcg agcgcttctt cgccgccgtg tacgagcagg cccaggggac cggcgccccg
     1501 gtgcccgagg ccgacatcga agggggtggc cacggggacg aggcggaggt cgtcagcgcg
     1561 tcgagctgag atttctcctc aactctcccg gcggcgcgac cgacggggaa ccatggtgtt
     1621 gttgctgggg agcacgatcg gagtcctcgt ggtgggcgca gcggcgggcg cccgtcaccg
     1681 ggcccgtcac cgttgggtca gcccgtacac cgacctggcc accgccatgc gggcggtcac
     1741 ccggaccgag gccagcgagt tccgcccgac gggcctggtg gcgctccgcc ccgcccccat
     1801 gccgcctcgg gccgacatcg tcgacctcgg ctccgtgcgg gccctgcgtc accaggagtc
     1861 cgccgccgtc tgagatctcc tggtcggagg gcgtccgccc cggccgtcgg cgtagtagcg
     1921 acaggaacgc gttctactag ggtcgtcccg ctactccccg aaccccagga gacatccatg
     1981 cgtggagcgc tcatgcgcaa cgccggtgac gacgccctcg aggtcgtcga cggcctcgag
     2041 atcggtgagc ccggccccgg tcaggtcaag gtcaagatcg tcgccaccgg ggtctgtcac
     2101 tcggacctgt cggtcctcaa cgggaccatc ccacagccgc caccggtgat cctcggccac
     2161 gagggtgccg ggatcatcac gtccgtcggc gagggcgtgc ccggcctggc cgagggcgac
     2221 cacgtgatca tcgcctggtc gcccccgtgc ggcacctgtg ccgactgtgt cgaccgcaag
     2281 tcgccgcacc tgtgccaggc cttcctcttc aacatggcca tccccaagta caccctcgac
     2341 ggggtcgacg tctacgggat ggccggcgcc ggtacgtggg ccgaagagac gatcatgccc
     2401 tggcagggcg ccatcaggat cgacgacgac gtccctttcg aggtggcctc gctgatcggc
     2461 tgcgcggtca tgaccggggt aggagccgcc atcaacacgg ccaaggtcga gcccggctcg
     2521 accgtcgtgg tcttcggctg cggtggcgtc ggcatcagcg tgatccaggg ggcccgcgtg
     2581 tgc
//
LOCUS       DASTFV010000020         2490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_78684, whole genome shotgun sequence.
ACCESSION   DASTFV010000020 DASTFV010000000
VERSION     DASTFV010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78684"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VFP02_00305"
     CDS             <1..848
                     /locus_tag="VFP02_00305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET9541491.1"
                     /translation="QAAVAEMQAEIVELGHQADEDAVDLYMNPDPTLTVMDTDDLTQA
                     TRRDALLSTIASRHSDVLDRLGGLEIDLVDAEARTRDAAAAVEAERAEVAALLAEYQG
                     TLREQQRLESALTARIDAVQAEAAALAEEEASIRAALAQASQAPTTGPGGSVQTPPSA
                     AGLQWPADGTITGVFGEDRGDHIHAGIDIANSSGTPIYAANSGTVLGGCGGGYGNCVL
                     IDHGDGMVTLYAHQTSIFVGAGQHVDRGQNIGTMGCTGSCTGPHLHFETRINGVPYDP
                     MQYLP"
     gene            858..1643
                     /gene="proC"
                     /locus_tag="VFP02_00310"
     CDS             858..1643
                     /gene="proC"
                     /locus_tag="VFP02_00310"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET9541492.1"
                     /translation="MRLQLVGGGRMGEALLAGLLGAGWAPAGEVRVVEALAPRRAELT
                     ARFPDIEVTDTVGPAEGTVLATKPGDIATACAALGADAGRVLSIAAGVRLADLEGALP
                     SGTPVVRAMPNTPALVGAGAAAIAAGTAATDDDLAWAESILSAVGIVVRVAEPLLDAV
                     TGLSGSGPAYVFLVAEALIEAGVLVGLPRPVAAELAGQTLLGSARLLVEGDDGPEALR
                     AAVTSPGGTTAAGLRALEQAGVRAAFLDAVVAATERSRELGGV"
     gene            1690..1878
                     /locus_tag="VFP02_00315"
     CDS             1690..1878
                     /locus_tag="VFP02_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9541493.1"
                     /translation="MAEERARFLTVAEVADLLRVSSMTVYRLIEAGDLRALRVGKSYR
                     INADDLESFLGPRYTQTG"
     gene            1875..>2490
                     /locus_tag="VFP02_00320"
     CDS             1875..>2490
                     /locus_tag="VFP02_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HET9541494.1"
                     /translation="MTRYSTVSFLSDYGTGDEFVGVVKSVIRSIEPAVAVIDITHEIP
                     PHDIRAGSLALARAAQYLVPGVVLAVVDPGVGTTRRAVAVEVGNGQSVLVGPDNGLLA
                     AAVGMVGGADRVIELTNADYHLPSPGATFDGRDLFAPVAAHLCAGVDLEEFGDPIEAT
                     SLQPGMIPLPFAEGEAVAAQVLWIDRFGNAQLNVGPDDVEHLGDR"
BASE COUNT          329 a          942 c          885 g          334 t
ORIGIN      
        1 cccaggccgc ggtggccgag atgcaggccg agatcgtcga gctgggtcac caggccgacg
       61 aagacgccgt cgacctctac atgaaccccg acccgacgtt gacggtgatg gacaccgacg
      121 acctgaccca ggccacccgg cgggatgccc tgctgtcgac catcgcctct cgccacagcg
      181 acgtgctcga ccggctcggc ggcctcgaga tcgacctggt cgacgccgag gcccggaccc
      241 gtgacgccgc cgccgcggtc gaggccgagc gggccgaggt cgcggccctc ctggccgagt
      301 accaaggcac gctccgcgag cagcagcgac tggagtcggc gctgaccgcc cggatcgacg
      361 ccgtccaagc cgaggccgcg gccctggccg aggaggaggc ttccatccgg gccgccctgg
      421 cccaggcgtc ccaggccccg accaccggtc ccggcggatc cgtccagacc ccgcccagcg
      481 ccgccggtct gcagtggccg gccgacggca cgatcaccgg ggtcttcggc gaagaccgcg
      541 gcgaccacat ccatgccggc atcgacatcg ccaacagctc cgggacgccc atctacgccg
      601 ccaactcggg caccgtgctc ggcggctgcg ggggcgggta cggcaactgc gtcctcattg
      661 accacgggga cggcatggtc acgttgtacg cgcaccagac gtccatcttc gtgggcgcgg
      721 gtcaacacgt cgaccgaggc cagaacatcg ggacaatggg ttgcaccggc agctgcaccg
      781 ggccccacct ccacttcgag acccgaatca acggggtccc ctacgacccc atgcagtatc
      841 tcccttagcg cggcgacgtg cgcctgcaac tggtcggagg cgggcggatg ggcgaagccc
      901 tcctggccgg cctgctcggc gccggctggg ctcccgccgg cgaggtccga gtcgtcgagg
      961 ccctggcgcc tcgccgggcg gagttgacgg cgcggttccc cgacatcgag gtgaccgaca
     1021 ccgtcggccc ggccgagggc accgtcctgg ccaccaagcc cggcgacatc gccacggcct
     1081 gcgccgcgct cggcgccgac gccggtcggg tcctgtccat cgccgccggg gtccgcctgg
     1141 ccgatctcga gggcgcactg cccagcggca ccccggtggt gcgggccatg cccaacaccc
     1201 cggccctggt cggggccgga gcggcggcca ttgccgcggg cacggccgcc accgacgacg
     1261 acctggcctg ggcggagtcg atcctgtcgg cggtcggcat cgtggtccgg gtggccgagc
     1321 ccctgctcga cgccgtcacc ggcctgtccg ggtccggccc ggcctatgtc ttcctggtgg
     1381 ccgaagcgct gatcgaggcc ggcgtcctgg tcggcctgcc gcggcccgtc gcggccgagc
     1441 tggccgggca gaccctgctc ggctcggccc ggctgctggt cgagggcgac gacggtcccg
     1501 aggccctgcg cgccgcggtc acctcaccgg gcgggacgac ggctgcgggc ctgcgagcgc
     1561 tcgagcaggc cggcgtcagg gccgccttcc tggacgccgt ggtcgccgcc accgagcgct
     1621 cccgcgagct gggcggggtc tgagcggctg gtttagggtg gccccagcgg agttagggga
     1681 aagcacaacg tggctgagga gcgcgcccgg ttcttgaccg tggcggaggt cgccgatctc
     1741 cttcgggtgt cgagcatgac ggtgtatcgc ctcatcgagg cgggggacct gcgcgccctg
     1801 cgggtgggca agtcgtaccg catcaacgcc gacgacctcg agtccttcct cggcccccgg
     1861 tacacccaga ccgggtgact cgctactcga ccgtctcctt cctctcggac tacggcaccg
     1921 gcgacgagtt cgtcggcgtg gtcaagtccg tcatccgttc gatcgagccc gccgtcgcgg
     1981 tcatcgacat cacccacgag atcccgcccc acgacatccg ggccgggagc ttggccctgg
     2041 cccgggccgc ccagtacctg gtgcccggtg tggtgctggc ggtcgtcgac cccggcgtcg
     2101 gcaccacccg ccgggccgtc gccgtcgagg tcggcaacgg ccagtcggtc ctggtcgggc
     2161 ccgacaacgg cttgctggcc gccgcggtgg gcatggtggg cggcgccgac cgggtgatcg
     2221 agctcaccaa cgccgactac cacctgccct cgccgggggc gaccttcgac ggacgggacc
     2281 tcttcgcgcc ggtcgccgcc cacctgtgtg ccggcgtgga cctggaggag ttcggcgacc
     2341 cgatcgaggc caccagcctg caaccgggga tgatccccct gcccttcgcc gagggggagg
     2401 cggtggccgc ccaggtcctg tggatcgacc gcttcggcaa cgcccagctc aacgtgggcc
     2461 ccgacgacgt cgagcacctg ggtgaccggg
//
LOCUS       DASTFV010000021         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_78724, whole genome shotgun sequence.
ACCESSION   DASTFV010000021 DASTFV010000000
VERSION     DASTFV010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78724"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFP02_00325"
     CDS             complement(<1..543)
                     /locus_tag="VFP02_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9541495.1"
                     /translation="MRTINHWINGQSVATSAERTGAVYDPATGQQAAQVSYATGDDVD
                     AAVRAAQAAFPAWRDTSVVKRARVMFAFRDLLEKHRDELARIIASEHGKVVPDAAGEV
                     QRGLEVVEVASGIPHLLKGDFSENVSTEVDCFSIRQPLGVAAGITPFNFPIMVPMWMH
                     PIAIACGNAFVLKPSERDPSV"
     gene            complement(570..>2279)
                     /gene="iolD"
                     /locus_tag="VFP02_00330"
     CDS             complement(570..>2279)
                     /gene="iolD"
                     /locus_tag="VFP02_00330"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR04377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HET9541496.1"
                     /translation="RQYADVLPYHMARNEQSMVHIASGYAKHRNRLATFACTSSIGPG
                     ATNMVTGAATATVNRLPVLLLPGDIFASRVAPPVLQQQESSGGPDVSANDAFRPVSRF
                     WDRINRADQLPGSLLEAMRVLTDPAETGAVTLALPQDVQTEAFDYPEELFARRVWHVA
                     RPVPEPAMLARAAEVLRGAGRPLIVAGGGVVYAEATGALRTLVEATGVPVAETYAGKG
                     SMPYDHPSALGAIGVTGTDGANAMARDADVVLGVGTRWADFATASHSVFANPEARFVN
                     VNVAPVDAFKHAAVPVVADARVALEALAEALAGWTVDDDWRRAAAGHNRDWDAKVEAV
                     YRAGHGPLPSQGEVIGAVNDAAEARDVVVCAAGSMPADLHKLWRTRDPKQFHLEYGNS
                     CMGYEIPGAIGIRLADPGREVFAMVGDAGYLMNPGELVTAVAEGIKIIVVVVQNHGYA
                     SIGSLSEASGNGRFGTRFRYREADGTDGEVLPVDLAANAASLGAKVLTPRTIGELADA
                     LKEARASDQPVVVHVETDPAEYVTIGGAWWDVAVAEVSTSESVQRARTDYEAGKADQR
                     HYL"
BASE COUNT          289 a          785 c          885 g          320 t
ORIGIN      
        1 caccgacggg tcgcgctcgg acggcttgag cacgaaggcg ttgccgcagg cgatggcgat
       61 cgggtgcatc cacatcggca ccatgatggg gaagttgaac ggggtgatcc cggcggccac
      121 ccccagcggc tggcggatcg agaagcagtc gacctcggtg gagacgttct cggagaagtc
      181 gcccttgagc aggtggggga tgccggaggc gacctccacc acctccaggc cccgctggac
      241 ctcgccggcg gcgtcgggca cgaccttgcc gtgctcggag gcgatgatcc gggccagctc
      301 gtcgcggtgc ttctcgagca ggtcgcggaa ggcgaacatg acccgggccc gcttgacgac
      361 cgaggtgtcg cgccaggccg ggaaggcggc ctgggcggcc cggacggcgg cgtcgacgtc
      421 gtcgccggtg gcgtaggaga cctgggcggc ctgctgcccg gtggccgggt cgtagacggc
      481 accggtgcgc tcggccgagg tggcgacgct ctggccgttg atccagtggt tgatggtccg
      541 catgggcagt cctcccgcca ggttgggcgc tacaggtagt gccgctggtc ggccttgccg
      601 gcctcgtagt cggtccgggc ccgctgcacg gactcggagg tggagacctc ggccacggca
      661 acgtcccacc atgcgccacc gatggtcaca tactcggccg ggtcggtctc gacgtggacg
      721 acgacgggct ggtccgacgc gcgcgcctcc ttgagcgcgt cggccagctc cccgatggtg
      781 cgcggggtca ggaccttggc cccgaggctg gccgcgttgg cggccaggtc gacggggagg
      841 acctcgccgt ccgtgccgtc ggcctcgcgg tagcggaacc tggtgccgaa gcggccgttg
      901 cccgacgcct ccgacagcga cccgatggac gcgtacccgt ggttctggac cacgacgacg
      961 atgatcttga tcccttcggc caccgccgtc accagctcgc ccgggttcat caggtagccg
     1021 gcgtcgccga ccatggcgaa cacctcgcgg ccggggtcgg ccagccggat cccgatggcg
     1081 ccggggatct cgtagcccat gcaggagttg ccgtactcca ggtggaactg cttggggtct
     1141 cgggtccgcc acagcttgtg caggtcggcg ggcatgctcc cggcggcgca gacgaccacg
     1201 tcgcgggcct cggcggcgtc gttgacggcg ccgatgacct cgccctggct gggcagcggc
     1261 ccgtggccgg cccggtagac ggcctcgacc ttggcgtccc agtcccggtt gtgcccggcg
     1321 gcggcccgcc gccagtcgtc gtcgacggtc cagccggcca gcgcctcggc cagcgcctcc
     1381 agggccaccc tggcgtcggc gaccaccggg acggcggcgt gcttgaaggc gtccaccggg
     1441 gcgacgttga cgttgacgaa gcgggcctcg gggttggcga agacgctgtg ggaggcggtg
     1501 gcgaagtcgg cccagcgggt gccgaccccg aggaccacgt cggcgtcgcg ggccatggcg
     1561 ttggccccgt cggtgccggt cacgccgatg gcgcccaggg ccgaggggtg gtcatagggc
     1621 atcgagccct tgccggcgta ggtctcggcc accgggacgc cggtcgcctc gaccagggtc
     1681 cggagcgccc cggtggcctc ggcgtagacg accccgccgc cggccacgat cagcggcctg
     1741 ccggcgcccc gcagcacctc ggccgccctg gccagcatcg ccggctcggg cacgggccgg
     1801 gccacgtgcc agacccggcg cgcgaacagc tcctcggggt agtcgaaggc ctcggtctgg
     1861 acgtcctggg gcagggccag ggtgaccgcg ccggtctcgg ccgggtcggt cagcacccgc
     1921 atggcctcca gcagcgaccc ggggagctgg tcggcccggt tgatccggtc ccagaagcgc
     1981 gacaccggcc ggaaggcgtc gttggcgctg acgtccggcc cgcccgacga ctcctgctgc
     2041 tggagcacgg gcggggcgac ccgggaggcg aagatgtcgc cgggcagcag cagcacgggc
     2101 aggcggttga cggtggcggt ggccgccccg gtgaccatgt tggtcgcgcc cggcccgatc
     2161 gacgaggtgc aggcgaaggt ggccaggcgg ttgcggtgct tggcgtagcc ggaggcgatg
     2221 tggaccatgc tctgctcgtt gcgggccatg tggtagggga gcacgtccgc gtactggcg
//
LOCUS       DASTFV010000022         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_78891, whole genome shotgun sequence.
ACCESSION   DASTFV010000022 DASTFV010000000
VERSION     DASTFV010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78891"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VFP02_00335"
     CDS             complement(<1..1112)
                     /locus_tag="VFP02_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9541497.1"
                     /translation="MIVDFGDSPEEAAFRERLRAWLRENDPGLPASSTSDDYWEGQAA
                     WHQALYDGGFFALSWPTAIGGHGLPTVYETIVDDELAAAGTPPRPSLGYLVKGILEHG
                     NEDVQQRFLPGVVNGRERWCQGFSEPDAGSDLASLRTRAERDGDEYVITGHKVWTSYS
                     DVADWCLVLARTDPDVPKHKGISAFVVSMHQPGIEQRPLRMINGVTREFGEVSFDGAR
                     VPASDMIGAPGEGWRLAMTVVSHEREPGELGYVARYRKVVNQLADQVREEPDRFGPEV
                     VRDLGWAMVEAEMLRLHVFRRLSDTLDGISHGPEGSVDKLLMTNVEQAVGHAALAVGG
                     AAHDGGDDTWLKVYLYSRAQSVMGGTSQVQRNLVATR"
     gene            complement(1093..2136)
                     /locus_tag="VFP02_00340"
     CDS             complement(1093..2136)
                     /locus_tag="VFP02_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET9541498.1"
                     /translation="MDVRLSPEQRALRDSAAQLVDRLAPSTVGALDDGERTAKLDAAV
                     AASGWRELRVAADGDEPLASGVEVAIVAEELGRRLADAPFLGPTLAAELRRLTGAPPA
                     TTAETVALAADLSSLALGTDGTHPSGVVAIDALGASSALVLLADGDGHRLGTVELTTA
                     SEPVDLTRPSVVPDPSATVAPLADPARALDPDALSAWTALGLATTCADLVGTMQGAVT
                     LATTYAADRRQYGVPVGSFQAVQHLLADAFVATEGSRSVALHAAWAVDALPAEDALAA
                     AAVAKAYCARAARSVCETAIQVHGGIGNTWECLAHVHLRRALLSSEVLGGVGASLARV
                     LEHHGLGGDRGLR"
BASE COUNT          309 a          800 c          862 g          305 t
ORIGIN      
        1 cgggtggcga cgaggttgcg ttggacctgt gaggtcccgc ccatgacgct ctgggctcgg
       61 ctgtagaggt acaccttcag ccacgtgtcg tcgccgccgt cgtgggcggc accgccgacg
      121 gcgagggcgg cgtggccgac ggcctgctcg acgttggtca tgagcagctt gtcgaccgag
      181 ccctccgggc cgtgggagat gccgtcaagg gtgtcggaca agcgccggaa gacgtgaagg
      241 cgcagcatct cggcctcgac catggcccac cccaggtcgc ggaccacctc ggggccgaac
      301 cggtcgggct cctcccggac ctggtcggcc agctggttca ccaccttgcg gtaacgggcg
      361 acgtagccga gctcgcccgg ctcccgctcg tggctgacga cggtcatggc caggcgccaa
      421 ccctcgccgg gggcgccgat catgtccgac gcggggacgc gggcgccgtc gaaggagacc
      481 tcaccgaact cccgggtgac gccgttgatc atccgcagcg gccgctgctc gatgccgggc
      541 tggtgcatcg agacgacgaa ggccgagatc cccttgtgct tggggacgtc ggggtcggtt
      601 cgggccagca ccaggcacca gtcggcgacg tcggagtagc tggtccacac cttgtggccg
      661 gtgatgacgt actcgtcgcc gtcgcgctcg gcccgggtgc gcagggaggc gaggtcggag
      721 ccggcgtcgg gctcgctgaa gccttggcac caacgttccc ggccgttgac cacgccaggc
      781 aggaaccgct gctgcacgtc ctcgttgccg tgctcgagga tgcccttgac cagatagccc
      841 aagctcggtc ggggcggtgt gccggccgcg gccagctcgt cgtcgacgat ggtctcgtac
      901 acggtgggca gcccgtgtcc gccgatcgcg gtaggccagg acagggcgaa gaagccgccg
      961 tcgtagaggg cctggtgcca cgcggcctgg ccctcccagt agtcatccga cgtggacgac
     1021 gccggtagtc cggggtcgtt ctcccggagc caggcgcgga gccgttcccg gaacgcagcc
     1081 tcctcgggcg agtcaccgaa gtccacgatc acctcccagg ccgtggtgct cgaggacgcg
     1141 ggcgaggctg gccccgaccc cacccaggac ctcgctggac agcagcgcgc ggcgcaggtg
     1201 gacgtgggcc aggcactccc aggtgttgcc gatgccgccg tgcacctgga tggcggtttc
     1261 gcacacgctc cgggcggccc gggcgcagta ggccttggcc accgcggcgg ccgccagggc
     1321 gtcctcggcc ggcagcgcgt ccaccgccca ggccgcgtgg agggccacgc tgcgagaccc
     1381 ctcggtggcc acgaaggcat cggccaggag gtgctggacg gcctggaacg agccgaccgg
     1441 gaccccgtac tggcgtcggt cggcggcgta ggtcgtcgcc agcgtcaccg caccctgcat
     1501 ggtcccgacc aggtcggcgc aggtagtggc caggccgagt gccgtccacg ccgagagggc
     1561 gtcggggtcg agggcccggg ccgggtcggc caacggagcg accgtcgccg acggatccgg
     1621 gacgacggac ggccgggtga ggtcgaccgg ctcggacgcg gtggtgagct cgaccgtgcc
     1681 cagtcggtgg ccgtcgccgt cggccaggag cacgagagcc gacgaggcgc cgagggcgtc
     1741 gatggccacc acccccgacg gatgggtgcc gtcggtcccg agagccaggg atgacaggtc
     1801 ggcggcgagg gcgaccgtct cggccgtcgt ggccggcggc gcgcccgtca ggcggcgaag
     1861 ctccgcggcc agggtcggtc cgaggaacgg ggcgtcggcg agcctgcggc ccagctcctc
     1921 ggccacgatg gccacctcga cccccgaggc cagcggctcg tcgccgtcgg ccgccacccg
     1981 cagttcgcgc cagccactgg ccgccacggc ggcgtcgagc ttggccgttc gctccccgtc
     2041 gtcgagggcg ccgaccgtcg acggtgcgag gcggtcgacc agctgcgccg ccgagtcgcg
     2101 cagtgctcgt tgctcgggcg aaagacggac gtccatcagc gcccacttcc tgacgccacc
     2161 ggctgggcgg tccactcgcc ccggaggacg cggcggagga ccttgccgga ggggagcctc
     2221 gggatggcgt cgacgacgac gatctcccgg agctgcttgt agttggccag cgtgtc
//
LOCUS       DASTFV010000023         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_82303, whole genome shotgun sequence.
ACCESSION   DASTFV010000023 DASTFV010000000
VERSION     DASTFV010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82303"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /locus_tag="VFP02_00345"
     CDS             complement(<1..1447)
                     /locus_tag="VFP02_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9541499.1"
                     /translation="MGDLWHDERLYNAGELVDAERGATYENINPATEEVLGVAADASV
                     GDIDRAIAAARRAFDETGWATDVELRARCLRQLHTALLDNLEEIRATTVAEVGTPVAL
                     TYGPQLDAPVGFLPYYADLAESYDWSEDLGDAEPMGIPSHRWIEKEPIGVVGAITAWN
                     FPHQLNIAKIAPAFAAGNTVVLKGAPATPWAALILGRLVAEHTDIPPGVLNVITSGRN
                     DIGEAITTDPRVDLITFTGSTAVGKRIMAAASDTVKKVFLELGGKSAIVALDDCDMNA
                     AAGTGAFGVCTHAGQGCAITTRLLLPRSRYDEGVEIAKTVLESMPYGDPTDPGNFMGP
                     LVTDVQRERVEGLIRTAQDEGATLVTGGKRPAHLDKGWFVEPTLLVDVDPDSTIAQQE
                     VFGPVLAVIPFDDDDDAVHIANNSIYGLSGAVNSGDIERAKAVGRRIRSGTMSINGGV
                     YYGADVPFGGYRQSGLGREMGIEGFEEYLETK"
     gene            1633..2121
                     /locus_tag="VFP02_00350"
     CDS             1633..2121
                     /locus_tag="VFP02_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET9541500.1"
                     /translation="MGDGGFDAIREPLARLLRLGASRKVYARQVAAAGVELSPPGAAL
                     LARIVEDGPITLGDLSAAADMDPGAVSRQVKALEADGLVERGGRAGDARIRVMRATSA
                     GRRVRQRIAAVQDRHMADVLDGWSAADRTTFARLLDRFVEDLRSVQYRPTDQSNHHRR
                     SA"
     gene            2121..>2717
                     /locus_tag="VFP02_00355"
     CDS             2121..>2717
                     /locus_tag="VFP02_00355"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9541501.1"
                     /translation="MPMPGDIGAIDLMIGFPAEDARRHYDYMKANFKDEASAEMEFPA
                     EYMFKDVPNHKESEDDAIAITLGEMDKYGVAMAMTSCRPDVNQRALSEHPDRFFASIE
                     IDPNDITKAVRRVRKAHEEHGIKAVTTFPSGCNPPVPVSDRRYYPIYQTCIDLGLPIV
                     ANAGIAGPRLPSHVQDVMHFDQVCYDFPELVIVMRHGAE"
BASE COUNT          403 a          952 c          943 g          419 t
ORIGIN      
        1 acttcgtctc caagtactcc tcgaacccct cgatgcccat ctcgcggccc aggccggact
       61 ggcggtaccc cccgaacggg acgtcggcgc cgtagtagac gccgccgttg atcgacatgg
      121 tgccggagcg gatccggcgg ccgacggcct tggcccgctc gatgtcgccg gagttgaccg
      181 cgccggacag gccgtagatc gagttgttgg cgatgtggac ggcatcatcg tcgtcgtcga
      241 aggggatgac ggccagcacg ggcccgaaga cctcctgttg ggcgatggtg ctgtcggggt
      301 cgacgtcgac gagcagggtc ggctccacga accacccctt gtccaggtgc gccgggcgct
      361 tgccgccggt gaccagggtc gccccctcgt cctgggcggt gcggatcagg ccctcgaccc
      421 gttcccgctg gacatcggtg accaatggac ccatgaagtt ccccgggtcg gtggggtcgc
      481 cgtagggcat cgactccagc acggtcttgg cgatctcgac gccctcgtcg tagcgggagc
      541 ggggcaggag cagccgggtg gtgatggcgc agccctggcc ggcgtgggtg cagacgccga
      601 aggcgccggt gccggcggcg gcgttcatgt cgcagtcgtc gagggccacg atggcggact
      661 tcccgccgag ctccaggaac accttcttca ccgtgtcgga cgccgcggcc atgatccgct
      721 tgcccacggc ggtcgacccg gtgaaggtga tcaggtcgac ccgggggtcg gtggtgatgg
      781 cctcgccgat gtcattgcga cccgaggtga tgacgttcag cacgcccggc gggatgtccg
      841 tgtgctccgc gaccaggcgc ccgaggatca gggccgccca cggggtcgcc ggcgctccct
      901 tgaggacgac ggtgttgccg gcggcgaagg cgggcgcgat cttggcgatg ttgagctggt
      961 gcgggaagtt ccaggccgtg atggccccga ccacgccgat gggctccttc tcgatccagc
     1021 ggtgcgacgg gatgcccatg ggctcggcgt cgcccaggtc ctcgctccag tcatagctct
     1081 cggccagatc ggcgtagtag ggcaggaaac ccacgggcgc gtcgagctgg gggccgtagg
     1141 tcagcgcgac gggcgtgccg acctcagcca cggtcgtggc ccggatctcc tcgaggttgt
     1201 cgagcagagc agtgtggagc tggcgcaggc agcgggcccg cagctcgacg tcggtggccc
     1261 aaccggtctc gtcgaaggcc cgtcgggcgg cggcgatggc ccggtcgatg tcgccgacac
     1321 tggcgtcggc ggccacgccc agcacctcct cggtggccgg gttgatgttc tcgtaggtcg
     1381 ccccgcgctc ggcatcgacc agttcaccgg cgttgtagag ccgctcgtcg tgccagagat
     1441 cccccatgcg gggcgagcgt actcagcccg ctgaacaccg gttcaccggc aaccgacggt
     1501 tgccttctcg cgatcgctga ggtcattgcc gcaggggagc gtcgccgatg atgtgtccag
     1561 accgggcgcc ggctgaccgc cgtccggcct agtatttgac tgagtcaagc aactgcccta
     1621 cgttgcccgg tgatgggtga cggtggcttc gacgcgatcc gggagcccct ggcgcggctc
     1681 ctgcgcctgg gggccagtcg aaaggtgtac gcccgccagg tcgcggctgc gggggtcgag
     1741 ctctcgcccc cgggcgcagc gctgctggct cggatcgtgg aagacggccc catcaccctg
     1801 ggtgacctct cggcggcggc cgacatggac cccggcgccg tgagtcggca ggtcaaggcc
     1861 ctggaggccg acggcctggt cgagcgcggc ggccgcgccg gcgacgcccg gatccgggtc
     1921 atgcgggcca cttctgccgg ccgccgggtc cgccagcgca tcgccgccgt ccaggaccgc
     1981 cacatggccg acgtgctcga cggctggtcg gctgccgacc gcaccacctt cgcccgcctc
     2041 ctcgaccgct tcgtcgagga cctccggtcg gtgcagtacc gcccgaccga ccagtcgaac
     2101 caccacagga gatccgcctg atgcccatgc ccggcgacat cggggccatc gacctcatga
     2161 tcggcttccc ggccgaggac gcccgtcgtc actacgacta catgaaggcg aacttcaagg
     2221 acgaggcctc ggccgagatg gagttcccgg ccgagtacat gttcaaggac gtcccgaacc
     2281 acaaggagtc cgaggacgac gccatcgcca tcacgctcgg ggagatggac aagtacggcg
     2341 tggccatggc catgaccagt tgccgcccgg acgtcaacca gcgggccctg tccgagcacc
     2401 cggaccgctt cttcgccagc atcgagatcg accccaacga catcaccaaa gcggtgcgcc
     2461 gggtccgcaa ggcccacgag gagcacggca tcaaggcggt gaccaccttc ccctccggct
     2521 gcaacccgcc cgtcccggtc agcgaccgcc gctactaccc gatctaccag acctgcatcg
     2581 acctgggcct gccgatcgtg gccaacgccg gcatcgccgg gccccgcctg ccgtcgcacg
     2641 tgcaggacgt catgcacttc gaccaggtct gttacgactt ccccgaactg gtcatcgtca
     2701 tgcgccacgg ggccgag
//
LOCUS       DASTFV010000024         4355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_90068, whole genome shotgun sequence.
ACCESSION   DASTFV010000024 DASTFV010000000
VERSION     DASTFV010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90068"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..236
                     /locus_tag="VFP02_00360"
     CDS             33..236
                     /locus_tag="VFP02_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541502.1"
                     /translation="MGQPVTVVQKPTSRADVVRFEINRSITGMDPHRYVAGEEVLGDA
                     RPTRSPVAFWFTPAWRACTSTAM"
     gene            233..361
                     /locus_tag="VFP02_00365"
     CDS             233..361
                     /locus_tag="VFP02_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541503.1"
                     /translation="MITVDLGSGVTADGLLADIESLFTYYREGVEVVVPGGAETAD"
     gene            complement(603..749)
                     /locus_tag="VFP02_00370"
     CDS             complement(603..749)
                     /locus_tag="VFP02_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET9541504.1"
                     /translation="MSSSAAAATLARTGLDADTALLVGGALIVLGLLLVRYNREDDLQ
                     VDES"
     gene            829..1110
                     /locus_tag="VFP02_00375"
     CDS             829..1110
                     /locus_tag="VFP02_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541505.1"
                     /translation="MLDDATLELDVEEELLDGEELLEELLDGEELLDELELLELELDD
                     ELELLEELLDELEVPPVQTWLTRSAPLLAPGPAPAESKPVLPSVVAEMA"
     gene            1120..1371
                     /locus_tag="VFP02_00380"
     CDS             1120..1371
                     /locus_tag="VFP02_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541506.1"
                     /translation="MAGVDVWSLTTLKWTVPPLPTTDEAGVEPFQFRLELPTVALVPP
                     PLAPLTVRMKCQSTWPGLRETLPQSPGLLTLNSLVVAID"
     gene            1948..3234
                     /locus_tag="VFP02_00385"
     CDS             1948..3234
                     /locus_tag="VFP02_00385"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HET9541507.1"
                     /translation="MTDLLEAKGTRRGPIAVLLRPDVLVPVGVTILAGAIRLWNLAQP
                     ASLVWDEVYYGVDAANPFSADVELRAVHPPVGTKLIGLGVALFGEDAFGRRIMPWLAG
                     TLVVLLTYFLARRLLGSVWWSGLVALFVALDGLQIVNSRTAVLEIFLSLFVLAGTLLF
                     LRHLEASRASPGHLGPWTLACGLVMGLAVSTKWIVAPALAGMIVLTFLDADRTARRSL
                     VRPVLVAFVLTPLAVYVASYTGTWVATDTTPLDWVHRQLDILEFHRDLAAPHAFASRA
                     IGWPLMIRPVPYFTATRPDGQALEILAVGNPALWWSFVVALPVLLVSWWRERHRTVEV
                     VLAAWLVLYVPWVLVLRNQIFFYYLTPFVPFMALGLVWCLREATRRTRFGRPLTVMVT
                     GAVVVCAVVFLPLWLGLWVSPEHLERLLLFEGWHFR"
     gene            3269..4027
                     /locus_tag="VFP02_00390"
     CDS             3269..4027
                     /locus_tag="VFP02_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET9541508.1"
                     /translation="MASFRVGVQLHPQHCSIDELRAAWETADGLGVDSLWTWDHFYPL
                     YGDDTGAHFEGWTLLAAMAVTTTNAHIGLLVTGNSYRNPDLLADMARTVDQLSGGRTY
                     LGIGSGWFEKDYAEYGYEFGTAPGRLRELEASLKRIKARLAERNPGPAGELPVLIGGG
                     GEKVTLRLVAQYADAWNTFGPPSNFTHKNGVLDEWCEKLGRDPAEIERTVAIQGNEVD
                     NVDAYLEAGATHVIVMTGHPFDLGPVQTLLAKRS"
     gene            complement(4040..>4355)
                     /locus_tag="VFP02_00395"
     CDS             complement(4040..>4355)
                     /locus_tag="VFP02_00395"
                     /inference="COORDINATES: protein motif:HMM:NF017530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070273 - phosphatidylinositol-4-phosphate
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HET9541509.1"
                     /translation="DPLRPTRHPVTDPVAHAALLDEVRAAATGDGPLTRRDAVLLALA
                     GPCQLLEVVAPERADRKQAKRRIAAASEQVEAAAAVKHAIEAVAAATTAVVAASGAVY
                     AG"
BASE COUNT          585 a         1510 c         1524 g          736 t
ORIGIN      
        1 cgccgtccgc gccactacga tgccgctccg tcatgggaca accggtcacc gtcgtgcaga
       61 agccgacgtc acgcgctgac gtcgtccgct tcgagatcaa ccgttcgatc acgggcatgg
      121 acccgcaccg gtacgtcgcc ggtgaggagg tcctgggcga cgcccgcccg acgagatcgc
      181 ccgtcgcctt ctggttcacc ccggcgtgga gggcgtgcac atctacagca atgtgatcac
      241 cgtcgacctg ggttcgggcg ttaccgccga cggtctgctc gccgacatcg agagcctgtt
      301 cacctactac cgcgaaggcg tcgaggtcgt ggtccccggt ggcgcggaga cggccgactg
      361 acccagccct cccgcgcctg gtgccaggcg actgacccag cgcccccgcg cctggtgcca
      421 ggacgggaac cggggtttgt cgcagccggc gtttgaccgg cgggtcgtgt ctggcgctcc
      481 ctgcgctgac cgggatcgtc atcccccggc ctcggctcgc ggggagtcgc tgaagctgct
      541 caggctgcgc tgaccgggcc gcccgcgtca tgaacgcggg cggcccggtc ggcgagggcc
      601 cgtcagctct cgtcgacctg gaggtcgtcc tctcggttgt agcgcacgag gaggaggccg
      661 aggacgatca aggcgccgcc cacgagcagc gcagtgtcgg cgtcgagacc cgttcgggcc
      721 agggtcgcgg ccgcggcact gctgctcatc gaggtgtcgt ccggtccacc ccccgcaccg
      781 gggctggagc tggtcacacc gcccggatcc accgatggcg actgagatgt gctcgacgac
      841 gccacgctcg agctggacgt cgaggaggag ctactcgacg gtgaggagct gctggaggaa
      901 ctgctcgacg gtgaggagct gctcgacgag ctggagctgc tcgagctgga gctcgacgac
      961 gagctggagc tgctggagga gctgctcgac gagctggagg tcccacccgt gcagacgtgg
     1021 ctgacgcgca gtgcgccgtt gttggcaccg ggtcccgcgc cggccgagtc gaagccggtg
     1081 ctgccatcgg tcgtggccga gatggcgtag gggctactgg tggccggagt cgacgtctgg
     1141 tcgttgacga cgttgaagtg gaccgtgccc ccgttgccga ccacggacga ggccggggtc
     1201 gaaccgttcc agttcaggtt ggaattgccg accgtggcgt tggtgccgcc accgttggcg
     1261 ccactgacgg tcaggatgaa gtgccagagc acctggccgg gactgaggga gacgttgccg
     1321 cagtcaccgg ggttgttgac gttgaattcg ctcgtggtgg cgatcgactg acctcggtgg
     1381 gggtacttca gccagtaggt gaaggtcccg acgaccggac cgccctcccg gtcctgcgcc
     1441 gcggttgccg gcggaggtgc cggcgccgcg gtggtcgggg cgaccgtcgt ggccggggcc
     1501 gccgtggtcg ctggcgccgc ggtcgtcacc gggacggccg tggtctccgg gacgacggtg
     1561 gtcaccgaag cggcatccgc gtcgcccccc tcggcgactg ccggcgcagc cagcatgccc
     1621 agaaccagca ggaaggcgcc gatcgcggca aaactccatc cgccccgtcg ccaccggtcc
     1681 ggcgtcaccg ccgcgagaga aatcccagtc ttgcccgcca tcgccaatca cccatccctt
     1741 tgccgccgta ctgatcgctt accagaaggt acctggccgt tctgtccatt ggaagtaacg
     1801 gaaccaaaga gttttgtggc gctggtcact ccggccgcgg gccgtgcggg gatggggcac
     1861 gttcggtccg gccgtgcgac gatgatggcc tcgggcgcca gaccgagcac tcgggcaccg
     1921 tcaccggagt aacgaaggag tgcgactgtg accgatctgc tcgaggccaa gggcactcgg
     1981 cgcgggccca tcgccgttct cctccggccc gatgtgctcg tcccggtcgg cgtcacgatc
     2041 ctggcgggcg ccatccgact ctggaacctg gcccagcccg ccagcctggt ctgggacgag
     2101 gtctactacg gcgtcgacgc cgccaacccg ttctcggccg acgtcgaact gcgcgccgtc
     2161 caccctcccg tcgggaccaa gctcatcggt ctcggcgtcg ccttgttcgg cgaggatgcg
     2221 ttcgggcgcc gaatcatgcc gtggctggcc ggcacgctcg tcgtgctgct cacctacttc
     2281 cttgcccggc ggctgctcgg gtcggtgtgg tggtccggtc tggtggcgct gttcgtcgcc
     2341 ctggacgggc tccagatcgt caactcccga actgctgtcc tggagatctt cctctcgctg
     2401 ttcgtcctgg ctgggacgct gctgttcctg cggcatctcg aggcgagtcg cgcctcacca
     2461 ggtcacctcg ggccctggac actcgcctgt ggccttgtca tgggcctggc cgtgtcgacc
     2521 aagtggatcg tggcgcctgc actggcaggg atgatcgtgc tgacctttct cgatgccgac
     2581 cggacggctc gccggtccct cgtccgtccg gtcctcgtcg ccttcgtgtt gacgccgctc
     2641 gcggtctacg tggcgtccta caccggaacc tgggtcgcga ctgacaccac cccactcgac
     2701 tgggtccatc gccagctgga catcctcgag ttccatcgcg acctggccgc gccgcacgcg
     2761 ttcgccagcc gcgcgatcgg gtggccactg atgatccggc ccgtgccgta cttcacggcg
     2821 acgcgaccgg acgggcaggc attggagatc ctcgccgtcg ggaacccagc gctctggtgg
     2881 tccttcgtgg tggccctgcc cgtgttgttg gtttcgtggt ggcgcgagcg gcatcgcact
     2941 gtcgaggtcg tgttggccgc ctggttggtg ctgtacgtgc cgtgggtgct ggtcctacgg
     3001 aaccagatct tcttctacta cctcaccccg ttcgtcccgt tcatggcgct ggggctggtg
     3061 tggtgcctgc gtgaggcgac gagacgcacc cgcttcggtc gtcccctcac cgtgatggtc
     3121 acgggggcgg tcgtcgtctg cgcggtcgtg ttcctgccgc tctggctcgg gttgtgggtc
     3181 tcccccgagc acctcgaacg gctgctgctc ttcgagggct ggcatttccg gtaagggtcg
     3241 gcctgcaggc gctgggtagg ttcggccgat ggcttccttc cgtgtcggcg tccagctcca
     3301 tccgcagcac tgctcgatcg acgagctgcg ggcagcctgg gagacggccg acgggctcgg
     3361 ggtcgacagc ctctggacct gggaccactt ctaccccctg tacggcgacg acacgggcgc
     3421 ccacttcgag ggctggacgc tgctggccgc catggccgtc accaccacca acgcccacat
     3481 cgggctgctg gtcaccggca actcgtaccg gaaccccgac ctgctggccg acatggcccg
     3541 cacggtcgac cagctctccg gtggacgcac ctacctgggc atcgggtccg ggtggttcga
     3601 gaaggactac gccgagtacg gctacgagtt cggcaccgcg ccgggccgcc tgcgcgagct
     3661 ggaggcgtcg ctgaagcgga tcaaggcccg cctggccgag cggaaccccg gcccggcagg
     3721 cgagctaccc gttctcatcg ggggtggggg cgagaaggtc accctgcgcc tggtcgccca
     3781 gtacgccgac gcctggaaca ccttcggccc accctccaac ttcacccaca agaacggcgt
     3841 gctcgacgag tggtgcgaga agctgggccg tgaccccgcc gagatcgagc gcaccgtggc
     3901 catccagggc aacgaggtcg acaacgtcga tgcctacctc gaggccggcg ccacccatgt
     3961 catcgtgatg accggccacc ccttcgacct cggccccgtc cagaccctcc tggccaagcg
     4021 gagctgacgc ggccggacgt cagccggcgt agacggcgcc actggccgcc accacggcgg
     4081 tggtcgcggc ggctacggcc tcgatggcat gcttgaccgc ggcggcggcc tcgacctgct
     4141 cggaggcggc cgcgatccgg cgcttggcct gcttgcggtc ggcccgctca ggggccacga
     4201 cctcgaggag ttggcagggt ccggccaggg ccaggagcac ggcgtcgcgc cgggtcaacg
     4261 ggccgtcacc ggtcgccgcg gcccggacct cgtcgaggag ggcggcgtgg gcgaccgggt
     4321 cggtcaccgg gtggcgggtc ggccgcaggg gatcc
//
LOCUS       DASTFV010000025         3043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_95595, whole genome shotgun sequence.
ACCESSION   DASTFV010000025 DASTFV010000000
VERSION     DASTFV010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95595"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VFP02_00400"
     CDS             complement(<1..667)
                     /locus_tag="VFP02_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541510.1"
                     /translation="MEIDLLDGALYVNDPYPTYAWMRANAPAYWDAGNELWGISRYDD
                     IVEIEKHKDVFVNSNTTKGGYRPNIPADPSIIGLDDPKHAERRNLVSRRFTPRAVTRW
                     DDHVHETATALVDAAVAKGRVDVVEDLAAPLPAQMIGFLLGYPDEAWPRLKEWSERTI
                     HLGGGPRYHDDDGIAAAFEFAAATAELYEAKKACPADDVMSFYLEVEEGGLKHQGFGI
                     DEII"
     gene            complement(678..2417)
                     /locus_tag="VFP02_00405"
     CDS             complement(678..2417)
                     /locus_tag="VFP02_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9541511.1"
                     /translation="MYDLIIRNGTIVDGTGADARRGDVAVKDGKVVAMADQIEGEAAE
                     TVDADGKLVTPGFVDIHTHYDGQATWDDVLDPSAGHGVTTVVAGNCGVGFAPVHPGQE
                     QWLIQLMEGVEDIPGTALAEGMTWNWESFPEYLDVLDARSYAIDIGTQVPHGAVRGYV
                     MGERGARNEPATPEDIAQMAAIVKEGIEAGGMGFSTSRTLAHRAMDGEPVPGTYAAED
                     ELFGIGRSLGEVGRGVFELAPAAIDGMDAADPFKEIDWMRRLGAEIQRPVSFALLQLD
                     PAPELWRQLMDLSLEAIDGGANLRPQVASRPFGLLTGLQTNHVFAKRPTFMGLAHLPL
                     DELVVALKDPGTKAAILSEADAAPDPNVLFDGIGVFLGMMIDKTFVMGEPPDYEPTHE
                     TSIAGIAEATGRDKYELLYDRMLDNDGQSFLMLPLFNYSHGDHEAIREQLLHPSAVLG
                     LGDGGAHCGMICDASLPTYSLTHWARDRNRGEKLPLEWLIQKQTKDTAELYGMTDRGT
                     LEVGMRADLNVIDHQALTLHSPRLVRDLPAGGRRLIQEADGYVLTAVNGVVTRRDGVD
                     TGARPGRLVRSGA"
     gene            2581..>3043
                     /locus_tag="VFP02_00410"
     CDS             2581..>3043
                     /locus_tag="VFP02_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541512.1"
                     /translation="MGAVRYHLRSMLRTHWVGAVALAATVAVAGGVVLVLIAGTLRTA
                     SAPDRYAAARGDLYDTQMEQAEGRPLTAEVEALPAVESVVAATFFFGGILPADGNGDP
                     IEGITFAGTPEGFGGEIVDGRAPDPEVPGEFVASDLWIDTADAELGDEFLLV"
BASE COUNT          409 a         1010 c         1128 g          496 t
ORIGIN      
        1 agatgatctc gtcgatgccg aagccctggt gcttcaggcc cccttcctcg acctcgaggt
       61 agaaggacat gacgtcgtcg gccgggcagg ccttcttggc ctcgtagagc tcggcggtgg
      121 ccgccgcgaa ctcgaaggcg gcggcgatgc cgtcgtcgtc gtggtaccgg ggacccccgc
      181 cgaggtggat ggtgcgctcc gaccactcct tgaggcgggg ccaggcctcg tcggggtagc
      241 ccagcaggaa gccgatcatc tgggccggca acggtgccgc caggtcctcg accacgtcga
      301 cgcggccctt ggccaccgcg gcgtcgacca gcgccgtcgc cgtctcgtgg acgtgatcgt
      361 cccagcgggt gacggcccgg ggcgtgaagc ggcgggacac gaggttccgg cgctcggcgt
      421 gcttggggtc gtcgaggccg atgatggagg ggtcggccgg gatgttgggc cggtagccgc
      481 ccttggtcgt gttcgagttg acgaagacgt ccttgtgctt ctcgatctcg acgatgtcgt
      541 cgtagcggga gatgccccac agctcgttgc ccgcgtccca gtacgccggg gcgttggccc
      601 gcatccaggc gtaggtgggg tacggatcgt tgacgtacag cgccccgtcg agcagatcga
      661 tctccatggg cgcgccgtca ggcgccgctg cggacgaggc ggcccgggcg ggcgcccgtg
      721 tcgacaccgt cgcggcgcgt gaccaccccg ttgaccgcgg tcagcacgta gccgtcggcc
      781 tcttggatga gacggcggcc gccggcgggc aggtcgcgca ccagacgggg ggagtgcaac
      841 gtcagggcct ggtggtcgat gacgttgagg tcggcgcgca tgccgacctc gagggtgccc
      901 cggtcggtca tcccgtagag ctcggccgtg tccttggtct gcttctggat cagccattcc
      961 agcggcagct tctcgccccg gttgcggtcg cgggcccagt gggtcaggga gtaggtgggc
     1021 agggaggcgt cacagatcat cccgcagtgg gccccgccgt cgcccaggcc gagcacggcc
     1081 gaggggtgca gcagctgctc ccggatggcc tcgtggtcac cgtgggagta gttgaacagc
     1141 ggcagcatga ggaagctctg gccgtcgttg tcgagcatgc ggtcgtagag cagctcgtac
     1201 ttgtcccggc ccgtggcctc ggcgatgccg gcgatcgacg tctcgtgggt gggctcgtag
     1261 tcgggcggct cgcccatcac gaaggtcttg tcgatcatca tccccaggaa caccccgatg
     1321 ccgtcgaaga ggacgttcgg gtccggcgcg gcgtccgcct cgctgaggat ggccgccttg
     1381 gtgccggggt ccttgagggc caccacgagc tcgtcgaggg gcagatgggc caggcccatg
     1441 aaggtcgggc gcttggcgaa cacgtgattg gtctgcagcc cggtgagcag gccgaagggc
     1501 cgggaggcga cctgcgggcg caggttcgcc ccgccgtcga tggcctcgag ggagaggtcc
     1561 atcagctgac gccacagttc cggggccggg tcgagctgga ggagggcgaa gctgaccggt
     1621 cgctggatct ccgcgccgag gcggcgcatc cagtcgatct ccttgaacgg gtcggcagcg
     1681 tccatgccgt cgatggcggc cggggccaac tcgaacacgc cccgaccgac ctcgcccagc
     1741 gaccggccga tgccgaacag ctcgtcctcg gcggcgtagg tgccggggac gggctcgccg
     1801 tccatggccc ggtgggcgag ggtgcgggag gtcgagaagc ccatcccgcc ggcctcgatg
     1861 ccctccttga cgatggccgc catctgggcg atgtcctcgg gcgtggccgg ctcgttcctg
     1921 gcccctcgtt cgcccatgac atagccccgc acggcgccgt gggggacctg ggtgccgatg
     1981 tcgatggcgt agctccgggc gtcgagcacg tcgaggtact cggggaagct ctcccagttc
     2041 caggtcatgc cctcggccag ggcggtcccg gggatgtcct ccacgccctc catgagctgg
     2101 atcaaccact gctcctggcc ggggtggaca ggggcgaagc ccacgccaca gttgccggcg
     2161 accacggtgg tgacgccgtg gccggcggac gggtcgagca cgtcgtccca ggtggcctgg
     2221 ccgtcgtagt gggtgtggat gtcgacgaac ccgggggtga ccagcttccc gtcggcgtcg
     2281 acggtctcgg ccgcctcgcc ctcgatctgg tcggccatgg ccacgacctt gccgtccttg
     2341 accgccacgt caccgcggcg agcatcggct cccgtgccgt cgacgatcgt cccgttgcgg
     2401 atgatcaggt cgtacatggc ttcccccgaa gtagaactcg ttccagtttc catccagctt
     2461 aggccccggc cgtcagaggt ccgtcgacga gcgttgggcg ggctcggctg agggctgccc
     2521 ccccggggtc atggtgcagg cacccttgtc cccgccgcag tcggagggtt ggctgggaat
     2581 gtgggggccg tccgatacca cctgcggtcg atgctgcgca cgcactgggt gggcgcggtc
     2641 gccctggccg cgacggtggc ggtggccggt ggggtggtcc tcgtcctcat cgcgggaacc
     2701 ctccggacgg ccagcgcgcc agaccgctac gcggcggcgc gaggtgacct ctacgacacc
     2761 cagatggagc aggcggaggg ccgccccctg accgctgaag tcgaggcgct gcccgcggtc
     2821 gagtcggtgg tcgccgccac cttcttcttc ggcggaatcc tccccgcaga cggcaacggc
     2881 gatccgatcg aagggatcac gttcgccggc accccggagg gcttcggagg cgagatagtg
     2941 gacgggcgcg cacccgaccc cgaggtgccc ggcgagttcg tcgcttcgga cttgtggatc
     3001 gacacggccg atgccgaact gggcgacgag ttcctactcg tca
//
LOCUS       DASTFV010000026         3214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_100267, whole genome shotgun sequence.
ACCESSION   DASTFV010000026 DASTFV010000000
VERSION     DASTFV010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100267"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="VFP02_00415"
     CDS             complement(<1..1385)
                     /locus_tag="VFP02_00415"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HET9541513.1"
                     /translation="MPPSSPTPLTLDGPATVLAGRFTPEEHKTYRVLPVVVPEGTQRL
                     EVGYRWAPEHETVLDLGLWDTAGYRAAEGFRGWSGSRAGRQHQGEGPIWIETGAAARC
                     YGAGPIEPGVWWIELGAGEVSAEGSTYTVTVAAWSTASGGTGPATDPVDAGHVARAEP
                     GWYHADLHQHGRHSHPRAPEWDEFVARSRAAGLDLLPIVDYVTPWHWDELGAVQRANP
                     DLLVWPGREVITYFGHAVVLGETRGLVEYRHGFEDVDLGTIQRAAVDAGALFQVAHPT
                     TYPEALFGSHCRGCEFTLDDAIDWDLVTSIEVLTGPILLGEPPDYPNPFVPTALERWR
                     ALLAAGHRLTAVCGSDDKLGGEFGSSATALWCPELSRAGVIEALRAGHAYVRTHGALA
                     SPALEVRAGEAMMGDTVIGPTELEVTVVNGQGQELALLKDGVVVHTEDLSREEATWRT
                     EIAPDPASGPLG"
     gene            1408..2175
                     /locus_tag="VFP02_00420"
     CDS             1408..2175
                     /locus_tag="VFP02_00420"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET9541514.1"
                     /translation="MAVDPQTLFDLSGRVAIVTGGTRGIGRAVAEGLAAAGAKVVVAS
                     RKADACAETQAALEATGAEALGVPTHLGELDDLSALVRATVDRFGGVDIVVNNAANAV
                     AQPLGMLTAEAWEKSLAVNLRGPTFLVQEALPHLKDSAHGAVVNVISVGAFLFGAYTS
                     MYAAGKGGLLSMTRYMATEFAPLGIRVNALAPGSVDTDMVRNNSPETQERMATASKMG
                     RIATADEMVGPVLFLVSDASSYMTGQVLVVDGGLAPY"
     gene            2291..2971
                     /locus_tag="VFP02_00425"
     CDS             2291..2971
                     /locus_tag="VFP02_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541515.1"
                     /translation="MTAVVEEKVETPETVLELDTPIPITRRRIDHLLVWSGGAVAVVL
                     LAAGILLTWGSTFARDYVHDELTAQAITFPDAAGLEEEGRTDLVKYADEVVDSGTEAE
                     AYASYIAGHVEGIGQGQTYAELGGPQFAAEAALDEAIANDAPDDEVAALREEYNGIVA
                     QRDSMFRGEVLRGALLSTFAWYTVGQIAGIAAIAAFVGALVMGVLVLLGALHLRKMSK
                     HATAHATA"
     gene            complement(3035..>3214)
                     /locus_tag="VFP02_00430"
     CDS             complement(3035..>3214)
                     /locus_tag="VFP02_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541516.1"
                     /translation="AVVDWLTAEGVPDIRAHIHPDHTASARVARAAGLEPTDEWIGGE
                     VMWALPGSSRRQAKQ"
BASE COUNT          456 a         1174 c         1137 g          447 t
ORIGIN      
        1 cccagggggc cgctggccgg gtccggggcg atctcggtgc gccaggtcgc ctcctctcgg
       61 gacaggtcct cggtgtggac gacgacaccg tccttcagca gggccagctc ctgaccctgc
      121 ccgttgacca cggtgacctc gagctcggtc ggcccgatga ccgtgtcgcc catcatcgcc
      181 tcacccgccc ggacctcgag ggccgggctg gccagggccc cgtgggtgcg cacgtaggcg
      241 tgcccggccc gtagcgcctc gatcaccccg gcccgggaga gctccgggca ccacagcgcc
      301 gtggccgacg agccgaactc gccgcccagc ttgtcgtcgg agccgcagac cgccgtcagg
      361 cggtgccccg cggcgagcag ggcccgccag cgctcgaggg ccgtgggcac gaacgggttc
      421 gggtagtccg gcggctcgcc gagcaggatc ggtccggtca ggacctcgat gctggtgacc
      481 aggtcccagt cgatggcgtc gtccagggtg aactcgcatc cccggcagtg gctgccgaag
      541 agggcctcgg gatacgtcgt gggatgggcc acctgaaaga gggcgccggc gtcgacggcg
      601 gcccgctgga tggtccccag gtcgacgtcc tcgaacccgt ggcggtactc gaccaggccc
      661 cgggtctcgc ccaggacgac ggcgtggccg aagtaggtga tgacctcccg gccgggccag
      721 accagcagat ccgggttcgc ccgctgcacg gcgcccagct cgtcccagtg ccacggcgtg
      781 acgtagtcca cgatggggag caggtcgagg cccgcggccc gggagcgggc gacgaactcg
      841 tcccactccg gcgcccgggg gtgcgagtgc cgtccgtgct ggtgcaggtc ggcgtggtac
      901 cagccgggct cggctcgagc cacgtggccg gcgtcgacgg gatcggtggc cggaccagtg
      961 ccaccactgg ccgtcgacca ggcggccacg gtgacggtgt aggtcgagcc ctcggcactg
     1021 acctcaccgg cccccagctc gatccaccac acgccgggct cgatcggtcc cgccccgtag
     1081 caccgggcgg cggctcccgt ctcgatccag atcggcccct cgccctggtg ctgtcgcccg
     1141 gcccggctcc ccgaccagcc tcggaatcct tcggcggccc ggtagccggc ggtgtcccac
     1201 agcccgaggt cgagcaccgt ttcgtgctcc ggcgcccacc ggtagccgac ctccaggcgc
     1261 tgggtcccct ccgggaccac gaccggtagc acccggtagg tcttgtgctc ctcaggagtg
     1321 aaccgcccgg ccagcaccgt ggctggcccg tccagcgtga gcggcgtcgg tgacgagggg
     1381 ggcacggacg cagcgtaggt tcgggccatg gccgtggacc cccagacgtt gttcgatctc
     1441 tcgggccggg tggcgatcgt caccggaggg acccggggga tcgggcgggc cgtggccgaa
     1501 gggctggccg ccgcgggggc gaaggtcgta gtcgccagcc ggaaggcgga cgcctgcgcc
     1561 gagactcagg ccgccctcga ggccaccggg gcggaggccc tcggcgtccc cacccacctg
     1621 ggcgagctgg atgacctgtc cgccctggtc cgtgccacgg tcgaccgctt cggtggggtc
     1681 gacatcgtgg tcaacaacgc ggccaacgcc gtggcccaac cgctggggat gctcacggct
     1741 gaggcttggg agaagtcctt ggcggtgaac ctgcgcggac cgacctttct ggtccaggag
     1801 gcgctcccgc acctgaagga cagcgcccac ggcgccgtgg tcaacgtcat cagcgtgggg
     1861 gccttcctct tcggggccta cacgtccatg tacgccgcgg ggaagggcgg cctcctgtcc
     1921 atgacccgtt acatggccac cgagttcgcg ccgttgggca tccgggtcaa cgccctggcc
     1981 ccgggcagcg ttgacaccga catggtccgc aacaacagcc ccgagaccca ggagcgcatg
     2041 gccacagcct ccaagatggg ccgcatcgcg acagccgacg agatggtcgg cccggtcctc
     2101 ttcctggtca gcgacgccag cagctacatg acgggccagg tcctggtggt cgacgggggc
     2161 cttgccccct actgaggggt ggcgggacct tcggcccccc aagaccggac ctctggcccc
     2221 agacgccgac agccggcgcc cgtacgttcg acctcagagg atccacacga gggtcctaca
     2281 agggagaatc atgaccgcag tcgtcgaaga gaaggtcgag acgccggaga ccgttctgga
     2341 gctcgacaca ccgatcccga tcacgcgccg acgcatcgac cacctgttgg tgtggtcggg
     2401 cggcgccgtc gccgtcgtcc tgctggctgc cggcatcctg ctcacctggg gaagcacctt
     2461 cgcccgggac tacgtccatg acgagctgac cgcccaggcg atcaccttcc ccgacgccgc
     2521 agggctcgag gaagagggtc gcaccgacct cgtgaagtac gccgacgagg tggtcgactc
     2581 cggcaccgag gccgaggcct acgccagcta catcgccggc cacgtcgagg gcatcggcca
     2641 gggtcagacc tatgccgagc tcggtggtcc ccagttcgcc gctgaggctg cgctggacga
     2701 ggcgatcgcc aacgacgcgc cggacgacga ggtcgccgcc ctgcgtgagg agtacaacgg
     2761 catcgtcgcc cagcgggaca gcatgttccg gggcgaggtc ctccggggcg ccctgctcag
     2821 caccttcgcc tggtacaccg tcggccagat cgccggcatc gccgccatcg ccgccttcgt
     2881 cggggcgctg gtcatgggcg tgctggtcct cctgggggcc ctgcacctgc gcaagatgag
     2941 caagcacgcc acggcgcacg ccacggcctg atcctcggcc ttccggtccc cggccccccg
     3001 cagcgacggg gaccgggggc cggttcgcgt cctgtcattg cttcgcctgc cgccgcgacg
     3061 agcccggcag cgcccacatg acctcgccac cgatccactc gtcggtcggt tcgagcccgg
     3121 cggcgcgggc gactcgagcg gatgcggtgt ggtcggggtg gatgtgcgcc cggatgtcgg
     3181 gcacaccctc ggcggtcagc caatcgacca cggc
//
LOCUS       DASTFV010000027         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_107152, whole genome shotgun sequence.
ACCESSION   DASTFV010000027 DASTFV010000000
VERSION     DASTFV010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107152"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1989)
                     /locus_tag="VFP02_00435"
     CDS             complement(<1..1989)
                     /locus_tag="VFP02_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET9541517.1"
                     /translation="MSTAFVVLLLVLLNLGLGTRQELAARASVDALAQMQVPEARVIR
                     DGTLVAIPAIEVVPGDLLAVESGDIVAADGRLIRAANLETQEAALTGESAPIPKDAGV
                     VDGDDTPLGDRTDMVFQNTSVTRGTGSVIVTTTGMETEVGKIAGLLQSVDRTKSPLQQ
                     QLDGLTKWLGVVAWTALAIVVAIGAARGLPLEDLLLLGVAVAISAIPTGMPTFVQGML
                     SFGARRLADSKAIVRNLTDVETLGSTSAINTDKTGTLTMNQMTAVKLLFAGDWYTIEG
                     GGYGKTGAVQSAAGGGVPDFGPLSLALTLACDATVSDDGQVIGDPTEAALVVLAAKLG
                     ADAELTRAKYPRLAEVPFDSAYKYMATFQRYDDGSGEVLVEIVKGAPDVVLDLSSKAL
                     WHGEEVPIETARQELLDANRSLSENGLRVLGLAIRRIPVGELSAVQADPGAQVGELTF
                     AALVGIIDPLRAEAKDAVTAALAAGIDVRMITGDHAITAKAIGDQLHLGEGAMSGPEF
                     AALTDEELEARLPALHVFGRVAPEDKLRLVQIMQRQGLVVAMTGDAVNDAAALKQADI
                     GVAMGTGSEVSKQAAKMVLTDDNFATLVHAVALGRDIWHKITAYIRYQMSQLFGLVSM
                     FLVAALVDLNSGVALLPMQIIALNFFIAVFPVVAIMTDA"
BASE COUNT          286 a          668 c          757 g          309 t
ORIGIN      
        1 ggcgtcggtc atgatcgcca cgaccgggaa cacggcgatg aagaagttca gggcgatgat
       61 ctgcatcggc agcagcgcca cgccggagtt caggtccacc agcgcggcga cgaggaacat
      121 cgacaccaga ccgaagagct ggctcatctg gtaccggatg taggccgtga tcttgtgcca
      181 gatgtcgcgg ccgagggcga cggcgtgcac gagggtggcg aagttgtcgt cggtcaggac
      241 catcttggcc gcctgcttgg agacctcgct gccggttccc atcgccaccc cgatgtcggc
      301 ctgcttcagg gcggcggcgt cgttcacggc gtcgccggtc atggccacta ccaggccttg
      361 gcgctgcatg atctggacga gtcggagctt gtcttccggc gcgacgcgcc cgaagacgtg
      421 cagcgcgggg agccgcgcct ccagctcctc gtcggtgagg gcggcgaact cgggcccgga
      481 catggcgccc tcaccgaggt ggagctggtc gccgatcgcc ttcgccgtga tggcgtggtc
      541 gccggtgatc atgcgcacgt cgatgccggc cgccagggcc gcggtcacgg cgtccttggc
      601 ttcggcccgg agggggtcga tgatcccgac gagggcggcg aacgtgagct cgcccacctg
      661 agcccccggg tcggcctgga ccgccgacag ctcgccgacg gggatccgcc ggatggccag
      721 tccgagcacc cgcagcccgt tttctgacag ggaccggttg gcgtcgagga gctcctggcg
      781 ggccgtctcg atcgggacct cctcgccgtg ccagagcgct ttgctgctca ggtcgaggac
      841 cacgtcgggg gcgcccttga cgatttcgac cagcacctcg cccgacccgt cgtcgtagcg
      901 ctggaaggtg gccatgtact tgtaggccga gtcgaacggc acctcggcca gacgcgggta
      961 tttggcgcgg gtcagctcgg cgtcggcgcc gagcttggcg gccagcacga ccagggccgc
     1021 ctcggtcggg tcgccgatga cctggccgtc gtcggacacc gtggcgtcgc aggccagcgt
     1081 cagggcgagc gagagcgggc cgaagtccgg tacgccgcct ccggccgcgg actgcaccgc
     1141 gcccgtcttg ccgtagccgc cgccctcgat cgtgtaccag tcaccggcga acaggagctt
     1201 caccgcggtc atctggttca tcgtgagggt gccggtcttg tcggtgttga tggcgctggt
     1261 ggagccgagg gtctcgacgt cggtcaggtt gcggacgatg gccttggagt cggccagtcg
     1321 gcgggcgccg aaggacagca tcccctggac gaaggtgggc atgccggtcg ggatggccga
     1381 gatggccacc gcgaccccga gcagcaggag gtcctcgagg gggaggccgc gggcggcgcc
     1441 gatggcgacc acgatcgcca gcgcggtcca ggccacgacg ccgagccact tggtgagccc
     1501 gtcgagctgc tgctggagcg gtgacttggt gcgatcgacg gactgcagga ggccggcgat
     1561 cttgcccacc tcggtctcca tgccggtggt cgtcacgatc accgagccgg tgccccgggt
     1621 caccgaggtg ttctggaaga ccatgtcggt gcgatcgccc agcggcgtgt cgtcgccgtc
     1681 gaccacgccg gcgtccttcg ggatgggggc actctcgccg gtgagggcgg cctcctgggt
     1741 ctcgaggttg gcggcgcgga tgagccgccc gtcggcggcg acgatgtcgc cggactcgac
     1801 ggccagcagg tcgccgggga ccacctcgat ggcggggatg gccaccagcg tgccgtcccg
     1861 gatcacccgg gcctcgggca cctgcatctg ggccagggcg tcgacgctgg cccgggccgc
     1921 cagctcctgc cgggtcccga ggccgaggtt gagcagaacg agcaggagga cgacgaaggc
     1981 ggtggacacc tggccgatga tcaggctggc gatcgcgacc
//
LOCUS       DASTFV010000028         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_109274, whole genome shotgun sequence.
ACCESSION   DASTFV010000028 DASTFV010000000
VERSION     DASTFV010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109274"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="VFP02_00440"
     CDS             complement(<1..173)
                     /locus_tag="VFP02_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HET9541518.1"
                     /translation="MAEADSPPEPVESPYDRPGKWFVVHTQSGYEKKVKSNLEARTQS
                     MNMESRIYEVVIP"
     gene            complement(557..859)
                     /gene="secE"
                     /locus_tag="VFP02_00445"
     CDS             complement(557..859)
                     /gene="secE"
                     /locus_tag="VFP02_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET9541519.1"
                     /translation="MAMNRQTKRMLQKQGQLGDDGEPVAQKRKAPPPKNREQRVGPVQ
                     FLREVRGELRKVAWPTRDEVVKYSIVVLVAVVALTALIFALDYVFGEFVLKLLKAD"
     gene            complement(906..979)
                     /locus_tag="VFP02_00450"
     tRNA            complement(906..979)
                     /locus_tag="VFP02_00450"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(943..945),aa:Trp,seq:cca)
     gene            complement(1031..1678)
                     /locus_tag="VFP02_00455"
     CDS             complement(1031..1678)
                     /locus_tag="VFP02_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HET9541520.1"
                     /translation="MPGRPDRTVVVIGTGTDVGKTWVTAALARRLAAEGLAVAARKPA
                     QSFAPVDPPEGRDAAVLGAATGEDPDVVCPPHRSYAVPLAPPMAAALLARPPIALADL
                     LAELAWPEPCAIGLVETVGGVRSPISDDADGVALIEAVAPDLVVLVADAGLGTLNLVR
                     LTVAALAGPSGPPVVTILNRFDRTDGLHRDNLSWLRDRDGLDVVTEVDELADRLR"
     gene            complement(1662..1907)
                     /locus_tag="VFP02_00460"
     CDS             complement(1662..1907)
                     /locus_tag="VFP02_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9541521.1"
                     /translation="MWTGSVPAIRRPIVPVVLGDEARAVAASAALLERGLLVPAIRPP
                     TVPPGSSRLRITLSAAHTEEDVDALVVALEELDARAA"
BASE COUNT          304 a          725 c          811 g          316 t
ORIGIN      
        1 atggggatga ccacctcgta gatgcggctc tccatgttca tggactgggt acgagcctcg
       61 aggttggact tcaccttctt ctcgtagccc gactgggtgt ggaccacgaa ccacttgccg
      121 ggacggtcat aggggctctc gacgggctcc gggggcgagt cggcctcggc caggaggtcg
      181 tcctcggtga ggacctcggc gtcggcctcg acggtgtcct cgtcgacggt ctcggcctcg
      241 acctcggtct cgatctggtg gtcacccagg ccacccagcg cgtccagggg gttggccgcg
      301 acgggttcgt tgaggatgtc ggagtgggcg tggtccgcct cggtctcgaa ggactcgctc
      361 ccgcgtccac ccaacagggc cgcggggtcc tcctcggccg gggcgtcgtc gtcggccggc
      421 gcgtcggtct cggtcacggc atcttcggtc acggcatctt cagtcgctgc atcttcagtc
      481 gctgcatctt cggtcacggc ttcgtcagcc ccgtcggtgg gttcggtgtc ggaggtcaag
      541 gtcacagtcg cttcgatcag tccgctttga gcagtttgag gacgaactcg ccgaagacgt
      601 agtccagggc gaagatcagg gcggtcagcg ccacgacggc caccagcacc acgatcgagt
      661 acttgacgac ctcgtcccgg gtgggccagg ccaccttgcg cagctcgccc cgcacctcgc
      721 ggaggaactg caccgggccg acacgctgct cgcggttctt gggcggcggc gccttgcgct
      781 tctgcgcgac gggctcaccg tcgtcgccga gctgcccctg cttctggagc atgcgcttgg
      841 tctgtcggtt catcgccatg ggcgtgtctg atcagggctc gtaggtggtt cgggacagtg
      901 cagagcaggg caggagggac tcgaaccccc aaccttcggt tttggagacc ggcgctctac
      961 caaattgagc tactgccctc cggaggtcgg caggcaggat agcgcgaggg gtcgatcttg
     1021 ccccctcggt tcaccgcagt cggtcggcca gctcgtcgac ctcggtgaca acgtcgaggc
     1081 cgtcacggtc gcggagccac gacaggttgt cccgatgcag gccgtcggtc cggtcgaagc
     1141 ggttgaggat cgtgaccacc ggtgggcccg acgggccggc cagcgcggcc acagtgagcc
     1201 gcacgaggtt gagggtgccg aggccggcgt cagccacgag cacaaccagg tcgggggcca
     1261 cggcctcgat gagggcgaca ccgtcggcgt cgtcgctgat cggcgagcgc accccgccga
     1321 cggtctcgac caagccgatg gcacacggct cgggccacgc cagctcggcc aggaggtcgg
     1381 ccagagcgat cggcgggcgg gccagtaagg ccgcggccat cggcggggcc agagggacgg
     1441 cgtaggaccg gtggggcggg cacacgacgt cagggtcctc acccgtggcc gcccccagca
     1501 cggcggcgtc ccgaccctcc ggcgggtcca ccggggcgaa ggactgggcg ggcttgcggg
     1561 cggcgacggc caggccttcg gcggccaggc ggcgggccag ggcggcggtg acccaggtct
     1621 tgccgacgtc cgtgccggtc ccgatgacga cgaccgtgcg gtcaggccgc ccgggcatcc
     1681 agctcctcga gggcgaccac cagcgcgtcg acgtcctcct ccgtgtgggc tgcggagagg
     1741 gtgatgcgga ggcgggagct gccgggcggg accgtcggcg gccggatcgc cgggaccagc
     1801 agaccccgtt ccaggagagc ggcggaagcg gcgacggccc gagcctcgtc gccgaggacc
     1861 acggggacga tggggcgacg gatggccggg acggagccgg tccacatggc tccgcagccg
     1921 ggccaccagg ccggtcccct ccggtgaccg caggacccgg accgcggcca gggccgcggt
     1981 ggtgtcggcg ggcgtggagg cggtggtgaa gatgtaggag cgggcccggt tgaccaggag
     2041 gtcgaccagg gcggccgggc ccgcgacgaa gccaccgagc gagcccagcg tcttggacag
     2101 cgtgcccacc cgcagcacct ccgccccgac gtccggcgcc aggtccgggc cgagca
//
LOCUS       DASTFV010000029         2037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_113672, whole genome shotgun sequence.
ACCESSION   DASTFV010000029 DASTFV010000000
VERSION     DASTFV010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113672"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VFP02_00465"
     CDS             complement(<1..846)
                     /locus_tag="VFP02_00465"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET9541522.1"
                     /translation="MSEQRVRVGVLGCGNVGAALVGLIGAQGDMIEARTGLRLEVAKV
                     AVRNMSRERPVELAEGVLTNDAGAVVDDPTIDLVVEVIGGIEPARELIVQAVKSGKPV
                     ITANKELLANHGAELFAVAEGASVDLLFEAAVAGGIPLIRPLRESLAGEQVHRVLGIV
                     NGTTNYILTRMSEDGATYGEALAEAQSLGYAERDPTADVEGYDAGAKAAIIATIAFGV
                     RVVAGDVYHEGISGITPADITFAQRLGYEVKLLAIAESAAGADGVTEVAVRVHPAMVP
                     SHHPLA"
     gene            931..1188
                     /locus_tag="VFP02_00470"
     CDS             931..1188
                     /locus_tag="VFP02_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541523.1"
                     /translation="MSQVDDMEAAARRLHAVAAVVGNALDPIVAYHRPDVWQGGRAVR
                     FGQELVDHRLLLRAAADQLVADARLLAARADLLRAIQALTP"
     gene            1287..>2037
                     /locus_tag="VFP02_00475"
     CDS             1287..>2037
                     /locus_tag="VFP02_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9541524.1"
                     /translation="MEDAFECIVTIRSDLTLAYANPSMAKLLGHAPEDTLGRPVADFV
                     HPDDLDRALAELSGLESWGVANGTTSFRLRHGDGSWVPCDVTAAAVTDGEEDLIAVYC
                     RPAGYQHAVDTVLTRLLSGGSRAETLAPLLDVFDWAVNDAHIAIAWHEEGRGHQSIAT
                     GLPPELTGGEADPGAPWADARVDFAGVHCPGPAALDPLRRATAEELGRGALWVEPITD
                     EGSGIAALVTVWSRLDGPPPETHAFSMALAKT"
BASE COUNT          275 a          807 c          680 g          275 t
ORIGIN      
        1 cgccagcggg tggtgggacg ggaccatggc cgggtggacc cgcaccgcca cctcggtgac
       61 gccgtcggcc cccgccgccg attcggcgat cgccagcagc ttgacctcgt agccgagacg
      121 ctgggcgaac gtgatgtcgg cgggggtgat gcccgagatg ccctcgtggt acacgtcccc
      181 cgccaccacc cggaccccga aggcgatggt ggcgatgatc gccgccttgg ccccggcgtc
      241 atagccctcg acgtccgcgg tcgggtcccg ctcggcgtag ccgaggctct gggcctcggc
      301 cagggcctcc ccgtaggtgg ccccgtcctc gctcatgcgg gtgaggatgt agttggtcgt
      361 cccgttgacg atccccagca cccggtgcac ctgctcgccg gccagggact cccgcagggg
      421 gcggatgagg gggatgccgc cggccacggc cgcctcgaag agcaggtcga cgctggcccc
      481 ctcggccacg gcgaacagct cggcgccgtg gttggccagc agctccttgt tggcggtgat
      541 gacgggcttg cccgacttca ccgcctgcac gatcagctcg cgggcgggct cgatgccacc
      601 gatgacctcg accacgaggt cgatcgtggg gtcgtcgacc acggcgccgg cgtcgttggt
      661 gagcaccccc tcggccagtt cgaccgggcg ctcgcgggac atgttgcgca ccgcgacctt
      721 ggccacctcc agccgcaggc cggtgcgggc ctcgatcatg tcgccctggg ccccgatcag
      781 gccgaccagc gccgccccga cgttgccgca gccgagcacc ccgacccgca cccgctgttc
      841 gctcacgcgg caacgctacc ggccgcaccc ctcgccgaac cgccccattc ccgccggtcg
      901 ccccttgaca agtttcggta tattccacgc atgagccagg tggacgacat ggaagcggcg
      961 gcgcggcgtc tccacgccgt cgccgcggtc gtcggcaacg cgctggaccc gatcgtggcc
     1021 taccaccgcc ccgacgtctg gcagggaggc cgagcggtgc gcttcggtca ggaactcgtc
     1081 gaccaccgac tcctgctccg ggccgcagcc gaccagctgg tggccgacgc ccgcctcctg
     1141 gccgcccgcg ccgacctcct ccgcgccatc caggccctca ccccctgacc accgatcgaa
     1201 accacccgat cggacgctca ccgcccgccc ccctcggccg acgggaagcc gtgatggaca
     1261 ggtcgggcgt tctctacgag tccctggtgg aggacgcgtt cgagtgcatc gtgaccatcc
     1321 ggtcggatct cacgctggcc tacgccaacc cgtccatggc caagctcctc ggccacgccc
     1381 ccgaggacac cctcgggcgg ccggtggcgg acttcgtcca cccggacgac ctcgaccggg
     1441 ccctggccga gctctccggc ctcgagagct ggggagtggc caacggcacg accagcttcc
     1501 ggctccggca cggcgacggc tcctgggtcc cctgcgacgt gaccgcggcc gcggtgacgg
     1561 acggcgagga ggacctcatt gccgtctact gccggcccgc cggctaccag cacgcggtcg
     1621 acaccgtgct cacccggctg ctgagcggcg gatcgcgagc agagaccctc gctcccctgc
     1681 tcgacgtctt cgactgggcg gtcaacgacg cccacatcgc catcgcgtgg catgaggagg
     1741 gccgcggcca ccagagcatc gccaccggcc tgccccccga actgaccggc ggcgaagccg
     1801 accccggtgc gccgtgggcc gacgcccgag tggacttcgc gggcgtccac tgccccggcc
     1861 cggcggccct ggaccccctc cgtcgggcca cggcggaaga actcgggcgc ggcgccctct
     1921 gggtcgagcc gatcaccgac gagggctcgg ggatcgcggc cctggtcacg gtctggagcc
     1981 gcctcgacgg cccgcccccc gagacccatg ccttcagcat ggccctggcc aagaccc
//
LOCUS       DASTFV010000030         3839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_121270, whole genome shotgun sequence.
ACCESSION   DASTFV010000030 DASTFV010000000
VERSION     DASTFV010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121270"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VFP02_00480"
     CDS             complement(<1..682)
                     /locus_tag="VFP02_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HET9541525.1"
                     /translation="MERRMTLHASILDLIGNTPMVDLSSFSPTPGVRIIGKLEGQNPG
                     GSVKDRIAKKMIEEAEADGSLTPGKTILEPSSGNTGIALAMIGKIKGYPVKIVLPGNV
                     SPERRQLLEAFGADIIDSPAEEGSNGAVRRAEALAADHPEWAFLYQYGNEANPRAHYE
                     GTGPEIWADCPEITHFVAGLGTSGTIVGVGTFLKEQNPAIEVFAVEPPIGETVQGLRN
                     MDEGYIPPV"
     gene            complement(715..1134)
                     /locus_tag="VFP02_00485"
     CDS             complement(715..1134)
                     /locus_tag="VFP02_00485"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HET9541526.1"
                     /translation="MTSDALNLDRGPWRAMLAHAYDGLPLEACGLLAGPPGRSAITVF
                     YPCRNAEASSRIYTVHPQDHLRADRDAEDRGLAIVGVMHSHTHTEAYPSPTDVAQAPD
                     PDWHYIIVSLRQPEPVLRSYRIVDGNIAEEPVVLIDR"
     gene            1169..1675
                     /locus_tag="VFP02_00490"
     CDS             1169..1675
                     /locus_tag="VFP02_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9541527.1"
                     /translation="MTQPLTPEDLVEIEAIKQVKYRYARCLDQKLWDQMGDLLTEDAV
                     AEYSAGKYRHEGRDAIVGWISDAMKAETFLSSHRMHHPEIRLTGPDTAHGTWALEDTV
                     VETAYGITIQGAAFYEDDYRKVDGEWKIEHTGYKRTYEELFPRASIEGLRVTASWWAT
                     GGASDLEA"
     gene            1706..2185
                     /gene="smpB"
                     /locus_tag="VFP02_00495"
     CDS             1706..2185
                     /gene="smpB"
                     /locus_tag="VFP02_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET9541528.1"
                     /translation="MARPDGVKVVASNRQARRNYEILDTFEAGLVLSGSEVKSLREAK
                     VTVSDAYARIIDHEAWVIGLHITPYSHAADTFGDANPERDRKLLLHRSEIDKIKARLD
                     QDRLTLVPLALYFKDGRAKLELGLGKGRTGVDKRHHIAARDAEREAQRDVARARRNE"
     gene            2265..2635
                     /gene="ssrA"
                     /locus_tag="VFP02_00500"
     tmRNA           2265..2635
                     /gene="ssrA"
                     /locus_tag="VFP02_00500"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2712..3287
                     /locus_tag="VFP02_00505"
     CDS             2712..3287
                     /locus_tag="VFP02_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9541529.1"
                     /translation="MAIEVRPATAFADVKTMVGPKRPDANVCWCLSYRLTSSKENQGL
                     VGPARGKRVQQLVRQRPPPGVLAYDGDEVVGWAAVHPRADTSFARNRKIPHVDDLEVW
                     SVWCIRVRPGHRREGISHHLLAGAVDFARSNGAPAIEGYPVDNKGAKVDLTMAYVGTM
                     SLFEQAGFAKRADTDSVLNGFPRVLMRLDLR"
     gene            3350..>3839
                     /locus_tag="VFP02_00510"
     CDS             3350..>3839
                     /locus_tag="VFP02_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541530.1"
                     /translation="MHDRELTDAEAAPVGTARIDPSAEPFPRAVLTPVAAAPPADVAA
                     AAISALPRHRFIVDDPALAAALISQGVTLVRASSLMVLRLPAPSPIPPGPGVELRPVQ
                     DTPFEYGEIVRRAYPPDHPDHELGDATPEGAVATIRGYLAGEIVGPLLTDASAEARLN
                     GVL"
BASE COUNT          630 a         1337 c         1311 g          561 t
ORIGIN      
        1 acacgggcgg gatgtacccc tcgtccatgt tgcgcaggcc ctgcaccgtc tcgccgatgg
       61 ggggctccac ggcgaagacc tcgatggccg ggttctgctc cttgagaaag gtgccgacac
      121 cgacgatcgt cccgctcgtg ccgagcccgg cgacgaagtg ggtgatctcc gggcagtcag
      181 cccagatctc cgggcccgtg ccctcgtagt gcgcccgggg gttggcctcg ttgccgtact
      241 ggtagaggaa cgcccactcg gggtggtcgg cagccaacgc ctcagcccga cgcacggccc
      301 cgttggaacc ttcctccgcc ggcgagtcga tgatgtcggc cccgaaggcc tcgagcagct
      361 ggcggcgctc gggcgacacg ttgcccggca ggacgatctt gaccggatag cccttgatct
      421 tgccgatcat ggccagagcg atgcccgtgt tgcccgacga cggctccagg atcgtcttgc
      481 caggcgtgag cgacccgtcc gcctccgcct cctcgatcat cttcttggcg atgcggtcct
      541 tgaccgatcc gcccgggttc tgaccctcga gcttgccgat gatgcgcacg cccggtgtgg
      601 ggctgaacga gctcaggtcg accatcgggg tgttcccgat caggtccagg atcgacgcgt
      661 gcaacgtcat tctgcgctcc accccctcga ttgttgaccg aacaggtagg gaaactaccg
      721 gtcgatcaga accacgggtt cctcggcgat gttcccgtcg acgatccggt acgagcgcag
      781 cacgggctcg ggctggcgca gggagacgat gatgtagtgc cagtcggggt cgggggcctg
      841 ggccacgtcg gtgggcgacg ggtaggcctc ggtgtgggtg tggctgtgca tcacgccgac
      901 gatggccagc ccccggtcct cggcgtcccg gtcggcccgc aggtggtcct gggggtgcac
      961 cgtgtagatg cggctggagg cctcggcgtt gcgacagggg tagaagacgg taatggcgct
     1021 tcggcccggc ggaccggcca gcaggccgca ggcctccagc gggaggccgt cataggcatg
     1081 ggccagcatc gctcgccacg gcccacggtc gaggttcagg gcatcactcg tcacggcggc
     1141 gcggatcgta ccggtactgt ccgcggcgat gacgcaaccc ctgacccctg aagacctggt
     1201 cgagatcgag gccatcaagc aggtcaagta ccggtacgcc cgctgcctgg accagaagct
     1261 ctgggaccag atgggcgacc tgctgaccga ggacgcggtg gccgagtact cggccggcaa
     1321 gtaccgccac gagggccggg acgccatcgt gggctggatc agcgacgcca tgaaggcgga
     1381 gaccttcctc tcctcgcacc ggatgcacca ccccgagatc cgcctcaccg gcccggacac
     1441 ggcgcatggc acctgggccc tcgaggacac ggtcgtggag acggcgtacg gcatcaccat
     1501 ccagggcgcc gccttctacg aggacgacta ccgcaaggtc gacggcgagt ggaagatcga
     1561 gcacaccggc tacaagcgca cctacgaaga gctcttcccc cgggcgtcga tcgagggcct
     1621 gcgggtcacg gcgtcatggt gggccaccgg tggggcctcg gatctcgagg cctgatccac
     1681 gccctcacgc gcttgactgg cggtcatggc ccgccctgac ggcgtcaagg tcgtggcgag
     1741 caaccgccag gcccgacgca actacgagat cctcgacacc ttcgaggccg gcctcgtgct
     1801 gagcggcagc gaggtcaagt cactgcggga ggcgaaggtc accgtcagcg acgcgtacgc
     1861 ccggatcatc gaccacgagg cgtgggtcat cggtcttcac atcacgccct acagccacgc
     1921 cgcggacacc ttcggcgacg ccaacccgga gcgagaccgc aagttgctcc tgcaccggag
     1981 cgagatcgac aagatcaagg cccgcctcga ccaggaccgc ctgacgctgg tacccctcgc
     2041 cctctacttc aaggacgggc gggccaagct ggagctcggc ctcggcaagg gccggaccgg
     2101 cgtcgacaag cgccaccaca tcgccgcccg ggacgccgaa cgggaggccc aacgcgacgt
     2161 ggcccgggcc cggaggaacg agtagccgtc ccagacctcc gtagactgga aaccccgggg
     2221 ccaaaggctc ccgggagttg cttgacatct gagagtgaca gcacggggct gatcggtttc
     2281 gacgtggaga ccgtcaggcg tgtgtgcgac cggagttgct cagactcctt aaacggggca
     2341 cccaaagaag cgccgaaggc gacttcgctc tcgccgcctg acctagtcag taggtagaga
     2401 gtcacagcga cccgtaaggg cacgcggggc ctgtccactg cagcccggca acagaagcag
     2461 tggaggaccg cagggagaga ccgctggcgg cgggaaagac cgctgacacc ggggaaagac
     2521 ccggcggtca tgaggacccg acctctcggc ggccgtaagc cgtagatcgg gaatggtcgt
     2581 agcgcgtacg tctcgcactt cgcggacgag ggttcgattc cctccagctc caccgccccc
     2641 gtgtccgggc ccgagcgaga gctcgggccc gatttgttct cggtcggcgc cgcaacgggc
     2701 agaggtccgg catggcgatc gaagtacgac cggccacggc gttcgccgac gtgaagacca
     2761 tggtcgggcc caagcggccc gacgcgaacg tgtgttggtg cctgagctac cgcctgacct
     2821 cctcgaagga gaaccaaggc ctggtcggcc ccgcccgggg caagcgggtg cagcaactgg
     2881 tccggcagcg gccgccgccc ggcgtgctcg cctacgacgg cgatgaggtg gtcggctggg
     2941 ccgcggtgca cccgcgcgcc gacacgagct tcgcccgcaa ccggaagatc ccgcacgtcg
     3001 acgatctcga agtctggtcg gtgtggtgca tccgggtgcg gccgggccac cgtcgagagg
     3061 gcatctcgca ccacctgctg gcgggggccg tcgacttcgc ccgctcgaac ggcgcgcccg
     3121 cgatcgaggg ctaccccgtc gacaacaagg gcgcgaaggt cgacctcacc atggcctacg
     3181 tcggcacgat gagcctgttc gagcaggccg ggttcgccaa gagggccgac accgactccg
     3241 tcctgaacgg ctttcctcgc gtcctcatgc ggctcgacct gcgctgagcg accgtgacgg
     3301 cgatgcccct cgcggcgaga tcagccgctg acctgtcacg atcggaggaa tgcacgatcg
     3361 cgagctgacc gatgccgaag cggcccccgt cggcaccgcg cggatcgatc cgagtgccga
     3421 gccgttcccc cgggccgtgc tgacacccgt cgccgccgca ccgccggccg acgtggccgc
     3481 agcggcgatc agcgcgctgc cccgccaccg gttcatcgtc gacgacccgg ccctcgcggc
     3541 tgcgctcatc agccagggcg tcacgctcgt ccgggcctcg tccctcatgg tcctccggtt
     3601 gccggctccg tctcccattc cccctggccc tggcgtcgag ctgcggccgg tgcaggacac
     3661 gccgttcgag tacggcgaga tcgtccgacg ggcctatcca cccgatcacc cggaccacga
     3721 gctcggtgac gcgacgcccg aaggcgcggt ggcgacgatc cgtggctacc tcgccggtga
     3781 gatcgtcggc ccgctcctga ccgacgcctc ggccgaggcg cggctgaacg gtgtcctcg
//
LOCUS       DASTFV010000031         5691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_122497, whole genome shotgun sequence.
ACCESSION   DASTFV010000031 DASTFV010000000
VERSION     DASTFV010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122497"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VFP02_00515"
     CDS             <1..915
                     /locus_tag="VFP02_00515"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET9541531.1"
                     /translation="SQVVVTNGGKQAYFEAIESIINPGDEVLLPAPYWTTYPEPIALA
                     GGTSVVLPTDEAAGFRVTVDQLEAARTERTKALLFVSPSNPSGAVYPRAEVEAIGRWA
                     VEHSLWIITDEIYEHLTYDDNDFTSLPVVVPEAADQTIVLNGVAKTYAMTGWRVGWII
                     GPPDLVEAASTLQSHLCGNVANVSQRATIAALDGGLDDVAMMRTEYDRRGKLIHRLLN
                     EIDGVSCMLPQGAFYAYPSFAGVLGRELAGQTPTTTLALADLILAEAKVAFVPGEAFG
                     SPGYGRFSFALDPEDMQEGIRRIAEFLA"
     gene            912..2909
                     /locus_tag="VFP02_00520"
     CDS             912..2909
                     /locus_tag="VFP02_00520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET9541532.1"
                     /translation="MTPLDRTTPYGAWSSPITTDLLVTGAVGLHEVQADGEDLWWDEG
                     RPEEGGRIQLVRRTPDGATTDVLPDGFAARTRVHEYGGGSWRVHEGVLFFSNWSDQRL
                     HRLDPGGEPRPLTPEGCRYADMDVAPDGTWLVTVREDHRGEGEARNEIVRIDTTTGDE
                     TVLVTGPDFVASPRLTDWHVVRLAWTQWNHPDMPWDAAEVWTGVLDGTELTEAQRLAG
                     GADEWASQPVWAGGRLWLTSDRDDVAGGNPLRFAEVHRWDGGPLEPVSDDSHGDLAPP
                     PWVFRMSSLVPDAEDPSQPGGPLGPPVSDPGALAWEAHGTAVVTRDGIDHLVRFEDQT
                     QVEIDAPHTAVSSLVPAPGGLAAVAASFTSEPAIVVGPPHELVVVRPPRDLGLRPEEI
                     SLPESIDFPSAGGRTAHALYYPPTNPDHAAPDGEKPPLLVDIHGGPTAAARSQFQLGI
                     QFWTSRGFAVVDVNYGGSTGYGRPFRDLLKGQWGVVDVEDCAAAATYLAERGDVDPAR
                     LAIRGGSAGGFTTLAALAFHDTFAAGVSKYGVADLAVLAEETHKFESRYLDSLIGPWP
                     EAEALYRERSPLFHADQISVPLLVLQGLEDEVVPPNQSELIVEALKANGVPVAYLAFE
                     GEQHGFRKAETIVRAAEAELSFYGQVFGFDPVGVTEPVELT"
     gene            complement(2924..3409)
                     /locus_tag="VFP02_00525"
     CDS             complement(2924..3409)
                     /locus_tag="VFP02_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541533.1"
                     /translation="MRRLLLTLLASGLAFAACGGDDGGETAASDPSTTTTTAPDGSTT
                     TTEPRPVDSTFVPEDVDALCADLEGLADIDPDADPTQAQVDRLRVIADTAPSGVAEPL
                     LDVATFGQLAVDAGATVDSLPEDAAAIQGAAVEAATILIAYGNEACGIDVPLFDTIAG
                     V"
     gene            3489..4925
                     /locus_tag="VFP02_00530"
     CDS             3489..4925
                     /locus_tag="VFP02_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET9541534.1"
                     /translation="MTDLGFRSASDLVAAIKAKEVGSRELLDHLLDRVDKHNPAVNAV
                     ITLDVERARAAADAADAATARGEDLGPLHGLPMTIKDVFETEGLRSTSGAPELMDHVP
                     DHDAGAVARLKDAGAIVFGKTNLPLYAGDLQTFNQPFGVTNNPWDTTRTTGGSSGGAA
                     AALAAGLTPLELGSDIGGSIRNPSHMCGTFGLKPSFGVVPQDGYLSGPSGLRTLDINV
                     VGPMARTVEDLGLAFDVLAGPGRPAAARDTTRVAAWFDTPRTPLDPAMRDVFLTAVGG
                     LRAGGLAVDDAARPDVDYDEATDLFVQLLYTAMTASMGVDDETWALAASVADTEPGDD
                     EPLLLRAGRAIAMRHHEWLRKDERRSQIRDRWADFFTRFDVLICPVIPLPAFPHQVDD
                     DPTGVINRMVTIAGTEVPHGELIYWCGLIGVAYLPSVVVPVGRTAAGLPVGVQVVADF
                     HQDRTALAVAQRITDDLGGFEAPPGYGA"
     gene            4933..>5691
                     /locus_tag="VFP02_00535"
     CDS             4933..>5691
                     /locus_tag="VFP02_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HET9541535.1"
                     /translation="MSDGGYPVFQVQEPTKDDFYAALADAGLTWEYDDILERHQHPGM
                     RVKEGTVALILHTVRFLESDDSIDVVQVGMVLGPTAGVVARWPGDGLDGAFGEEFAER
                     KDLILALIEEVHDRDTEAMRAIEERVDDCELEVLDANGQGTSAQSIYHLEHELLKVRR
                     SVGPMVAIMDRLARKADETENQVLAEWRDRYLRLVSQMEDIDNLLTSVLSVNLTLVSV
                     RQNEDNRKIAAWGAIGIAPTAMAGIWGMNFRHMPE"
BASE COUNT          836 a         2073 c         1976 g          806 t
ORIGIN      
        1 agccaggtcg tggtcaccaa cggcggcaag caggcgtact tcgaagccat cgagtcgatc
       61 atcaacccgg gggacgaggt cctccttccc gcgccgtact ggacgaccta tcccgagccc
      121 atcgccctgg ccggtggcac gtccgtcgtg ctcccgaccg acgaggcggc ggggttccgg
      181 gtcaccgtgg accagctcga ggcggcccgc accgagcgga ccaaggccct gctgttcgtg
      241 tccccgtcca acccgtccgg tgccgtctac ccgcgggccg aggtcgaggc catcggccgc
      301 tgggccgtcg agcacagcct ctggatcatc accgacgaga tctacgagca cctcacctac
      361 gacgacaacg acttcacctc gttgcccgtc gtcgtgcccg aggccgctga ccagaccatc
      421 gtcctcaacg gcgtggccaa gacctacgcc atgaccggct ggcgggtggg ttggatcatc
      481 ggcccgcccg acctggtcga ggccgcctcc acgctccagt cgcacctctg cggcaacgtg
      541 gccaacgtct cccagcgggc caccatcgcc gccctcgacg gcggcctgga cgacgtggcc
      601 atgatgcgga ccgagtacga ccgtcgggga aagctcatcc accggctgct caacgagatc
      661 gacggcgtca gctgcatgct cccgcagggg gcgttctacg cctacccgtc gttcgcgggt
      721 gtgctcgggc gtgagttggc cggccagacc ccgacgacga ccttggccct ggccgacctg
      781 atcctggccg aggccaaagt cgccttcgtg cccggcgagg ccttcgggtc gccgggctac
      841 ggccgattct ccttcgccct cgatcccgag gacatgcagg aaggcatccg ccgcatcgcc
      901 gagttcctgg cgtgacgccc ctggatcgaa cgacaccgta tggggcgtgg tcgtcgccga
      961 tcacgaccga cctgctggtc acgggtgccg tcggcctgca cgaggtccag gccgacgggg
     1021 aggacctctg gtgggacgag ggtcgaccgg aggagggcgg tcggatccaa ctggtccggc
     1081 gcacgccgga cggcgcgacg accgacgtcc tgcccgacgg ctttgccgcc cgtacccggg
     1141 tccacgagta cggcggcggc agctggcggg tccacgaggg cgtcctgttc ttcagcaact
     1201 ggtcggacca gcgcctccac cgcctcgacc cggggggcga gccgaggccg ctgactcccg
     1261 agggctgccg ctacgccgac atggacgtgg cccccgacgg cacctggctg gtgaccgtcc
     1321 gggaggacca ccgcggcgag ggcgaggcca ggaacgagat cgtccgcatc gacacgacga
     1381 cgggcgacga gaccgtgctg gtcaccggcc ccgacttcgt cgcctcgccc cggctgacgg
     1441 actggcacgt ggtgcggttg gcctggaccc agtggaacca cccggacatg ccctgggacg
     1501 ccgcagaggt gtggaccggc gtgctggatg ggaccgagct cacggaggcc cagcggctgg
     1561 ccggcggggc ggacgagtgg gcctcgcagc cggtctgggc cggcggtcgc ctctggctca
     1621 ccagcgaccg tgacgacgtc gccgggggga accccctgcg gttcgctgag gtgcatcgct
     1681 gggacggcgg ccccctcgag ccggtctcgg acgactcgca cggcgacctc gccccgcccc
     1741 cctgggtgtt ccggatgtcc tcgctcgtgc cggatgccga ggatccgtcg caacccggcg
     1801 ggccgctcgg gccaccggtt tccgaccccg gcgccctcgc ctgggaggcc cacggcacgg
     1861 cagtggtgac ccgggacggg atcgaccact tggtgcgctt cgaggaccag acgcaggtcg
     1921 agatcgatgc gccgcacacg gccgtctcgt cgctcgttcc cgcaccgggc ggcctggccg
     1981 ccgtggccgc ctcgttcacc agcgaaccag cgatcgtggt cggaccgccg cacgaactgg
     2041 tcgtcgtccg gccgcctcgc gacctcggcc tccggccgga ggagatcagc ctgccggagt
     2101 cgatcgactt cccgagcgcc ggcggccgca cggcgcacgc cctctactac ccgccgacga
     2161 acccggatca cgctgctccc gacggcgaga agccgccgct gctggtggac atccacggcg
     2221 ggccgaccgc cgcggcgcgg agccagttcc agctaggcat ccagttctgg acaagccggg
     2281 gcttcgccgt ggtcgacgtg aactacggcg gctccaccgg gtacgggcgg cccttccgag
     2341 acctgttgaa aggtcagtgg ggcgtggtcg acgtggagga ctgcgccgcg gcggcgacgt
     2401 acctggccga gcgaggcgat gtcgaccccg cccggctggc catccgtggc ggcagtgccg
     2461 gagggttcac cacgctggct gccctcgcgt tccacgacac gttcgccgcc ggtgtcagca
     2521 agtacggcgt ggccgacctg gccgtcctcg ccgaggaaac ccacaagttc gagtcccgct
     2581 acctggactc cctcatcgga ccgtggccgg aggccgaggc cctctaccgc gagcgctcgc
     2641 ccctcttcca cgccgaccag atcagcgttc ccctgctcgt cctgcagggc ctggaagacg
     2701 aggtcgtgcc cccgaaccag tccgagctca tcgtcgaggc cctgaaggcc aacggtgtcc
     2761 ccgtcgccta cctggccttc gagggcgagc agcacggctt ccggaaggcc gagaccatcg
     2821 tccgggcggc cgaggccgag ctgtccttct acggccaggt cttcggcttc gaccccgtcg
     2881 gcgtcaccga gcccgtcgag ctgacctgat cccagggctg gggtcagacg ccggcgatgg
     2941 tgtcgaagag ggggacgtcg attccgcagg cctcgttgcc gtaggcgatg aggatggtgg
     3001 cggcctcgac ggcggccccc tggatcgccg ccgcgtcctc cgggagcgag tcgacggtcg
     3061 cgccggcgtc aaccgcgagc tggccgaagg tggcgacgtc cagcagcggc tcggcgaccc
     3121 cggacggcgc ggtgtcggcg atgacgcgca gccggtcgac ctgcgcctgg gtcggatcgg
     3181 cgtccggatc gatgtcggcc agcccctcga gatcggcgca gagcgcgtcg acgtcctcgg
     3241 gcacgaacgt ggagtcgacc ggccggggct cggtggtggt cgtcgagccg tcgggcgccg
     3301 ttgtcgtggt cgtcgacggg tcggacgccg ccgtctcccc accgtcgtcc ccgccgcatg
     3361 ccgcgaaggc gaggcccgaa gccaggaggg tcagcagcag tcgacgcatg ctggagcgac
     3421 ggtaccggcg gctccggcga cctgggagga tggggttgca cgggctcgtt cccggaccaa
     3481 ggagggacat gaccgatctg ggattccgca gcgcatccga cctcgtcgcc gccatcaagg
     3541 cgaaggaggt gggcagccgt gagcttctcg accacctgct ggaccgcgtc gacaagcaca
     3601 acccggccgt gaacgcggtc atcaccctcg acgtcgagcg ggcccgggct gcggccgacg
     3661 cggccgacgc cgcgactgcc cggggcgagg acctcggccc tctccacggc ctgcccatga
     3721 ccatcaagga cgtgttcgag accgagggcc tccgctcgac gtccggcgcc cccgagctga
     3781 tggaccacgt ccccgaccac gacgccggtg ctgtggcccg cctgaaggac gccggggcca
     3841 tcgtcttcgg caagaccaac cttcccctct acgccggcga cctccagacc ttcaaccaac
     3901 ccttcggggt gaccaacaac ccgtgggaca cgacgcggac cacgggtggc tcgtcgggcg
     3961 gggcggccgc cgccctcgcc gccggcctca ccccgctcga gctcggctcc gacatcggtg
     4021 gctccatccg caacccgtcc cacatgtgcg ggaccttcgg cctgaagccc tcgttcggcg
     4081 tggtccccca ggacggctac ctgtcggggc ccagtggcct gcgcaccttg gacatcaacg
     4141 tcgtcggccc gatggctcgc accgtggaag acctcggtct cgcgttcgac gtgctggccg
     4201 gaccgggtcg tcccgccgcc gcccgcgaca cgacccgggt ggccgcttgg ttcgacacgc
     4261 cccgtacgcc gctcgacccg gcgatgcgtg acgtcttcct gaccgcggtc ggcggcctgc
     4321 gggccggcgg gttggccgtc gacgacgccg cccggcccga cgtcgactac gacgaggcca
     4381 ccgacctctt cgtccagctg ctctacacgg cgatgacggc atcgatggga gtcgacgacg
     4441 agacctgggc cctcgccgcc tccgtcgccg acaccgagcc gggagacgac gagcccctcc
     4501 tcctccgcgc cggccgggcc atcgccatgc gtcaccacga gtggctgcgc aaggacgagc
     4561 gccggagcca gatccgcgat cgttgggccg acttcttcac ccggttcgac gtgctgatct
     4621 gcccggtcat cccgctcccg gcctttcccc accaggtcga cgacgacccg accggtgtga
     4681 tcaaccggat ggtcacgatc gccggtaccg aagtgcccca cggggagctg atctactggt
     4741 gtgggctgat cggggtggcc tacctgccgt cggtggtggt gcccgtcggc cgcaccgccg
     4801 cgggcctgcc ggtcggcgtc caggtggtgg ccgacttcca tcaggaccgc acggccctcg
     4861 ccgtcgccca gcgcatcacc gacgacctcg gcgggttcga ggccccgccc ggctacgggg
     4921 catgatcggg gggtgagcga cggcggctac ccggtcttcc aggtccagga gccgaccaag
     4981 gacgacttct acgcagccct cgccgacgcc gggctgacct gggagtacga cgacatcctc
     5041 gagcggcacc agcacccggg gatgcgcgtc aaggagggca cggtcgccct gatcctgcac
     5101 accgtccggt tcctggagag cgacgacagc atcgatgtgg tgcaggtggg gatggtgctc
     5161 gggccgacgg ctggggtcgt ggcccgctgg cccggcgatg gtctcgacgg ggccttcggt
     5221 gaggagttcg ccgagcgcaa ggacctgatc ctggcgctca tcgaggaggt ccacgaccgc
     5281 gacaccgagg ccatgcgggc catcgaggag cgggtcgacg actgtgagct ggaggtgctc
     5341 gacgccaacg gccagggcac cagcgcccag tcgatctacc acctggaaca cgagctgctg
     5401 aaggtgcgac ggtcggtcgg cccgatggtg gcgatcatgg accgcctggc ccgcaaggcc
     5461 gacgaaaccg agaaccaggt gctggccgaa tggcgtgatc ggtacctgcg cctggtcagc
     5521 cagatggagg acatcgacaa cctgctgacc agcgtgctca gcgtcaacct gaccctggtc
     5581 agcgtccgcc agaacgagga caaccgcaag atcgcggcct ggggcgccat cggcatcgcc
     5641 ccgacggcga tggccgggat ctggggcatg aacttccgac acatgccgga a
//
LOCUS       DASTFV010000032         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_128249, whole genome shotgun sequence.
ACCESSION   DASTFV010000032 DASTFV010000000
VERSION     DASTFV010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128249"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1428
                     /locus_tag="VFP02_00540"
     CDS             211..1428
                     /locus_tag="VFP02_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9541536.1"
                     /translation="MSTGMPVVDGMAFDTMAQFSAVAGDLRDPYPELAEKRRDQPVEA
                     TNYASFIEGLEQTTYIVHRYEDVVAVLRDNVTYSSSAIRALMAPVMGDYVLVGMDEPE
                     HKRLRALVSVAFRQRSLAAWEPYAQSVVDGYLDQVVERGSAELVRELTFPYPIQVIAK
                     ILGVPPEDTGKFHEWAWWIINVAADPDKGIAASEAMKAYLAEVLDSRRADPGDDVISD
                     LVQAELDGEQLGDEEIFSFIRLLLPAGAETTYRATGNFLFGLLTHPDQLAALRDDRSL
                     MTQAVEESIRWESPLLITSRDSTVESEVAGTVIPAGCQVVAHVGSANHDDARWEDPEV
                     FDIFREQKPHAAFGFGPHMCLGMHLARMEMRVAVGAVLDRLDDVRLAPDAEDVHIHGE
                     RFRSPTSLPVLFG"
     gene            1438..1620
                     /locus_tag="VFP02_00545"
     CDS             1438..1620
                     /locus_tag="VFP02_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET9541537.1"
                     /translation="MEIRIEVDRCMGSGNCAFWAPATFDIGDDNIAFVIDPEGDPEDK
                     IRNAAEGCPTQAIILS"
     gene            1617..>3011
                     /locus_tag="VFP02_00550"
     CDS             1617..>3011
                     /locus_tag="VFP02_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET9541538.1"
                     /translation="MTLGVTEDHRALHETARRWVEARGLLGAARATLDAPTDTLPPFW
                     AEVAELGWLGLHVAEEQGGSGFGLAELAVILEELGRAVAPGPILPTVLASAVIDRLGD
                     TDTRATLLPGMADGSTPGAVAFASAESASEWTAVLGAATAALVVVPIGDDWWALPTEG
                     LTVEEHKSLDPTRRLATVRLDAAPEGHRLGPEPRPGRVRDLAATLLAAESAGGSAWCV
                     DTASAYAKDRQQFGRPIGQFQAVKHLCADMLGALELARAVAWDAARGGDDDEVALAAA
                     VAGAIAPEAFARCAQGCVQVHGGNGFTWEPDAHLFLRRARATRQLLGGEEAWNDLVVE
                     RARGGARRSMTVDLPPEAEGPRAEVRAFLEDLRDQNRAEWDLRIAEAGWLVPNWPSPW
                     GRDADPIEQLVIDEEFREAHVRRRHLQVGAWVLPTLIVHGNAEQQERFIGPTLAGKIL
                     WCQMFSEPGAGSDLA"
BASE COUNT          420 a         1111 c         1055 g          425 t
ORIGIN      
        1 gccgaccgcg tcctctgggc cagcgacttc ccgcaccccg acgccctgtt ccctgacgcg
       61 gccacgatgt tcgtggccaa ggcccgccgc caggggatcg ccgacggcga cctggccacg
      121 atcctctggg acggcccgct cgcgttctac gccctcggga ctcgactgac gacctaacct
      181 gacacgcgtg tcaggttcgg agggggagcg atgagcacag ggatgcccgt ggtcgacggg
      241 atggccttcg acacgatggc ccagttcagc gccgtggccg gtgacctccg ggacccgtac
      301 ccggagctgg ccgagaaacg tcgggaccag cccgtcgagg cgaccaacta cgcctcgttc
      361 atcgagggcc tcgagcagac cacctacatc gtccaccgct acgaggacgt cgtggccgtc
      421 ctgcgggaca acgtcaccta ctcgtcgtcg gccatccggg cgctgatggc cccggtgatg
      481 ggggactacg tgctggtggg catggacgag cccgagcaca agcggctgcg agccctggtc
      541 agcgtggcct tccgccagcg cagcctggcg gcgtgggagc cctacgccca gtccgtggtc
      601 gacggctact tggaccaggt ggtcgagcgg gggagcgccg agctggtccg tgagctcacc
      661 ttcccgtacc ccatccaggt catcgccaag atcctcgggg tccccccgga ggacaccggc
      721 aagttccacg agtgggcctg gtggatcatc aacgtggcgg ccgacccgga caagggcatc
      781 gccgcgtccg aggcgatgaa ggcctacctg gccgaggtgc tcgactcccg tcgcgccgac
      841 cccggcgacg acgtgatcag cgacctcgtg caggccgagc tggacggcga gcagctcggc
      901 gacgaggaga tcttctcgtt catccgcctg ctgctgcccg ccggcgccga gaccacctac
      961 cgggcgacgg ggaacttcct gttcggcctc ctgacccatc ccgaccagtt ggccgccctc
     1021 cgggacgacc ggagcctgat gacccaggcg gtcgaggagt cgatccgctg ggagtcgccc
     1081 ctgctcatca cgtcccgtga ctccacggtg gagtccgagg tggcaggcac ggtgatcccc
     1141 gcgggctgtc aggtcgtggc tcacgtcggc tcggccaacc acgacgacgc ccgttgggag
     1201 gaccccgagg tcttcgacat cttccgggag cagaagcccc acgccgcctt cggcttcggg
     1261 ccccacatgt gcctcggcat gcacctggcc cgcatggaga tgcgggtggc ggtcggcgcc
     1321 gtcctcgacc gcctcgacga cgtccgcctc gcccccgacg ccgaggacgt gcacatccac
     1381 ggcgagcggt tccgctcgcc gacgtcgctc cccgtcctct tcgggtagaa ccgcaccgtg
     1441 gagatccgca tcgaggtcga ccgctgcatg ggttcgggca actgcgcgtt ctgggcgccg
     1501 gcgaccttcg acatcggcga cgacaacatc gcgttcgtca tcgatcccga aggggacccg
     1561 gaggacaaga tccgcaacgc cgccgagggc tgcccgaccc aggccatcat cctctcgtga
     1621 ccctgggggt caccgaggac caccgagcgc tgcacgagac cgctcggcgc tgggtcgaag
     1681 cccgcggcct gctgggcgcg gcccgggcca ccctggacgc cccgaccgac accctgcctc
     1741 cgttctgggc cgaggtggcc gagctcggct ggctgggcct gcacgtcgcg gaggagcagg
     1801 gaggctccgg attcggcctg gccgagctgg ccgtcatcct cgaggagctc ggccgggcgg
     1861 tggcgcccgg gccgatcctg cccaccgtgc tcgcgtcggc ggtgatcgac cgtctcgggg
     1921 acaccgacac gagggcgacc ctcctcccgg gaatggccga cggctcgacc cctggggccg
     1981 tggccttcgc gtccgctgaa tcggcgtccg agtggaccgc ggtcctcggt gccgccaccg
     2041 ccgcgctcgt ggtcgtgccc atcggcgacg actggtgggc ccttccaact gaagggttga
     2101 cggtcgagga gcacaagagc ctcgatccca cgcgccgcct ggccaccgtt cgcctcgacg
     2161 ccgcgcccga gggccaccgg ctcggtcccg agccccgccc cggccgggtc cgggacctgg
     2221 ccgccacgct gctggccgcc gagtcggcgg gcggatccgc ctggtgcgtc gacacggcgt
     2281 cggcctacgc caaggatcgt cagcagttcg gtcgtcccat cggccagttc caggccgtca
     2341 agcacctctg cgccgacatg ctcggcgcgc tcgaactggc ccgggccgtg gcctgggacg
     2401 ccgcccgggg cggcgacgac gacgaagtgg cgttggccgc ggccgtggcc ggggccatcg
     2461 cccccgaggc cttcgcccgc tgcgcccagg gctgcgtcca ggtccacggc ggcaacgggt
     2521 tcacgtggga gcccgacgcc cacctgttcc tgcgccgggc cagggccacc cgccagctct
     2581 tgggcggcga ggaggcctgg aacgacctgg tcgtcgaacg ggcgcggggt ggggcccggc
     2641 ggtcgatgac cgtcgacctc ccacccgaag ccgaggggcc ccgggccgag gtgcgggcct
     2701 tcctggagga cctccgcgac cagaaccggg ccgaatggga cctccgcatc gccgaggccg
     2761 gctggctggt ccccaactgg ccctcgccct ggggccgcga cgccgacccg atcgagcaac
     2821 tggtcatcga cgaggagttc cgcgaggccc acgtccgtcg tcgccacctg caggtggggg
     2881 cgtgggtgct gcccacgttg atcgtgcacg gcaacgccga gcagcaggag cggttcatcg
     2941 gcccgacgtt ggcggggaag atcctgtggt gccagatgtt cagtgagccg ggcgccggct
     3001 ccgacctggc c
//
LOCUS       DASTFV010000033         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_139781, whole genome shotgun sequence.
ACCESSION   DASTFV010000033 DASTFV010000000
VERSION     DASTFV010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139781"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1076)
                     /locus_tag="VFP02_00555"
     CDS             complement(141..1076)
                     /locus_tag="VFP02_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541539.1"
                     /translation="MRRRSTLVLLVAVLLGTVAQPAHARPVAGCPMFPANNYWHADVS
                     DLPVHARSADWVRSIGLDAGLKADFGSGLWDGYPIGIPTNVVTRATPTYRVSFTWPDE
                     SNRGRYPIPAHPRIEGGGDRHLLTVNRDDCRLRELFAVRRANGRWRAGSGAVWNLRSN
                     ALRPAGWTSADAAGLPILPGLVRWTEVAAGEIDHAIRFTAPATRDRYVWPARHQAGDA
                     DPDLPPMGAWFRLKSSVDISGFHGPLRVILEALQTHGMILADNGSPWYMSGTPDRHWN
                     NDVLNSLDEYFTGRDFEAVDTSSLIVDENSGAVAP"
     gene            1138..1878
                     /locus_tag="VFP02_00560"
     CDS             1138..1878
                     /locus_tag="VFP02_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541540.1"
                     /translation="MEKAPPSRARLWLVLGPLVILVIAAQVGDAFAPTLAVEHPLTLI
                     TLNARNRNLILVTNSLDAVSFYTVGFLRLLASDPLFYILGFWYGDAGVQWVEKRSSSF
                     GNLLRTWEWMFQKAAWPLVAIAPNNFICLFAGAAGMTPAVFLTLNVIGTAVRLYLIRV
                     LGEAFEKPIQAVLDFIADYRLPLTMLTVAIVVITVIADRRSGKGERTAIASIEDELGT
                     AEDPVVHDRDDPAATEADVEDDSTGARE"
     gene            1875..>2207
                     /locus_tag="VFP02_00565"
     CDS             1875..>2207
                     /locus_tag="VFP02_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9541541.1"
                     /translation="MSRGTAVVTGASSGIGAATAAALASAGFDVVVGARRVEKLAEVA
                     EPIGARAIALDVTDDESVAAFAADIDECSVLVNNAGGALGLERIEDADLGHWQAMFDT
                     NVLGTLRVT"
BASE COUNT          326 a          749 c          810 g          322 t
ORIGIN      
        1 cggtccagct gccagaagcc ggcggcgatc atggccagga tcagcaccag cacgagcagg
       61 tgggacacga tccacttggg ttgccgggcg aaggcgtaca ccgcgcccga ggctaggacc
      121 cccctccccg atgaggtggg tcagggggcg acggcgccgg agttctcgtc gacgatcagg
      181 gaggaggtgt cgacggcttc gaagtcccgg ccggtgaagt actcgtcgag ggagttgagc
      241 acgtcgttgt tccagtgccg gtccggcgtg ccgctcatgt accagggcga gccgttgtcg
      301 gccaggatca tcccgtgggt ctggagggcc tcgaggatga cccggagggg gccgtggaac
      361 ccgctgatgt cgaccgagga cttgagccgg aaccacgccc ccatcggtgg taggtcgggg
      421 tcggcgtcgc cggcctggtg ccgggccggc cagacgtagc ggtcgcgggt cgcgggggcg
      481 gtgaagcgga tggcgtggtc gatctcgccc gccgccacct cggtccagcg gaccaggccg
      541 ggcaggatcg gtagcccggc ggcgtcggcc gacgtccatc ccgcgggccg caaggcgttg
      601 gagcgcaggt tccagacggc accggatccc gcgcgccaac gcccgttggc gcgccggacg
      661 gcgaacagct cgcggagccg gcagtcgtcc cggttcacgg tcagcaggtg gcggtcgccc
      721 ccgccctcga tgcgggggtg ggccgggatc gggtagcggc cccggttgct ctcgtcgggc
      781 cacgtgaagc tgacccggta ggtcggcgtg gcccgggtga cgacgttggt ggggatcccg
      841 atcgggtagc cgtcccagag gccggagccg aagtcagcct tcagcccggc gtcgaggccg
      901 atcgaccgga cccagtcggc ggagcgggcg tgcaccggca ggtcggacac gtcggcgtgc
      961 cagtagttgt tcgccgggaa catcgggcag ccggccaccg gacgggcgtg cgcgggctgg
     1021 gcgaccgtgc ccaagagcac ggccacgagc agcacgaggg tggatcgccg acgcatgacc
     1081 cctttcatcg aaccacggca atcgtccctt gaggggcctg cctgccacaa tcgaccgatg
     1141 gagaaggccc cgccgagccg ggcacggctg tggctggtgt tggggccgct cgtcatcctg
     1201 gtgatcgcgg cccaggtcgg ggacgcgttc gcgcccaccc tggcggtcga gcacccgttg
     1261 accctgatca cgctcaacgc ccggaaccgc aacctcatcc tggtcaccaa ctcactcgac
     1321 gcggtctcgt tctacacggt cggcttcctg cgcctgctgg cctcggaccc gctcttctac
     1381 atcctcggct tctggtacgg cgacgccggc gtgcagtggg tcgagaagcg ctcgagcagc
     1441 ttcggcaacc tcctgcggac gtgggagtgg atgttccaga aggcggcctg gcccctcgtc
     1501 gccatcgccc cgaacaactt catctgcctg ttcgccgggg cggcggggat gaccccggcg
     1561 gtcttcctga ccctgaacgt catcggcacc gcggtgcggc tgtacctcat ccgtgtgctc
     1621 ggcgaagcgt tcgagaagcc catccaagcc gtcctcgact tcatcgccga ctaccgcctg
     1681 cccctgacca tgctcaccgt cgccatcgtg gtcatcacgg tcatcgccga tcgccgctcg
     1741 ggcaagggcg agcggaccgc catcgccagc atcgaggacg agctcggcac ggccgaggac
     1801 ccagtcgtcc acgaccgcga cgacccggcg gccaccgaag ccgacgtgga ggacgattcg
     1861 acgggagccc gagaatgagt cgagggaccg cggtcgtcac cggggccagc tcggggatcg
     1921 gggcggccac cgcggcggcc ctggccagcg ccgggttcga cgtcgtcgtc ggggcccgcc
     1981 gggtggagaa gctggcggag gtggccgagc cgatcggcgc ccgggcgatc gcgttggacg
     2041 tgaccgacga cgagtccgta gccgccttcg ccgctgacat cgacgagtgc tcggtcctgg
     2101 tcaacaacgc cggtggggcg ctgggcctgg agcgcatcga ggacgccgac ctcgggcact
     2161 ggcaggccat gttcgacacc aacgtgttgg gcactctgcg ggtgacc
//
LOCUS       DASTFV010000034         2507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_140851, whole genome shotgun sequence.
ACCESSION   DASTFV010000034 DASTFV010000000
VERSION     DASTFV010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140851"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VFP02_00570"
     CDS             <1..869
                     /locus_tag="VFP02_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9541542.1"
                     /translation="HVLLEGVPGVAKTLASETLAKVVGGSFVRIQFTPDLLPADIVGT
                     RISRGSSESFDVELGPVFANFVLTDEVNRAPAKVQSALLEVMAERHVSIGGQTFPVPD
                     PFLVLATQNPIESEGVYQLPEAQRDRFLMNVPIGYPSPSEEVEIVHRMGVDPPAAAQV
                     LNPEQVLDLQAAADEVYVDPGVVDYAVNVVFATREPADHGMSDLTGMIAYGASPRASL
                     GLVAAGRALALLRGRDYALPQDVFDVAPDVLRHRLVLSYEALADGVDVNDVLIRVLST
                     VPAAPVAPARAG"
     gene            866..1807
                     /locus_tag="VFP02_00575"
     CDS             866..1807
                     /locus_tag="VFP02_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9541543.1"
                     /translation="MSPSGTAPPRVAGAPAEEVLRRLQIDVTRRLDGMLHGDFEGLVP
                     GRGSDPGDTRAYTPGDDVRRIDWNVTARMPEVHVRETIADRELATWVLLDRSASLDFG
                     TATCEKRDMALAATAAIGFITLRTGNRLGVVVAGDGDVRVLPPGTGRRHLLGTLHAAT
                     LASAAAGGSSVDLAAAIDALARAGQRRRGLMVVVSDFLAPGGWERSLRGLASRHEVLA
                     VEVVDPRELALPDVGVIELVDPETGAQREIHTSRDLRERYATAAGDQRQDIARRVRAA
                     GSDHLVLRTDRDWLLDIVRFVSRRKERRRVAAGTAHH"
     gene            1804..>2507
                     /locus_tag="VFP02_00580"
     CDS             1804..>2507
                     /locus_tag="VFP02_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9541544.1"
                     /translation="MSYLAPHWLWLLAVVGFLAVVYAVLQWRRRSTFTVRFTNLELLD
                     SVAPKRPGWRRHVVAGLLLVSLSLLVVALARPTRPDAAPLGPVLVLAIDTSLSMQATD
                     VDPNRLEAAQEAADGLVEGLSDNVRVGLVSFDNGATIQVTPTTDHDAVRDAIDGLSLD
                     EFTAIGEAIFAGLDAIAIAEQDQPEAAEDPAADEPPPSQIVLMSDGETTTGRPDVEAA
                     QAAFDAGVPVSTIAFGT"
BASE COUNT          317 a         1010 c          826 g          354 t
ORIGIN      
        1 gccacgtcct gctcgagggc gtgcccggcg tagccaagac gttggccagc gagaccttgg
       61 ccaaggtcgt cggcggctcc ttcgtccgca tccagttcac ccccgacctt cttcccgccg
      121 acatcgtcgg cacccgcatc tcccgcggct cgtcggagag cttcgacgta gagctcggcc
      181 ccgtgttcgc caacttcgtc ctcaccgacg aggtcaaccg ggcgccggcc aaggtgcagt
      241 cggccctgct cgaggtcatg gccgagcgcc acgtctccat cggcggccag acgtttcccg
      301 tgcccgaccc gttcctggtc ctggccaccc agaaccccat cgagtccgaa ggcgtctacc
      361 agttgccgga ggcccagcgg gaccggttcc tgatgaacgt gcccatcggc tacccgtcac
      421 cctccgagga ggtcgagatc gtccaccgca tgggcgtgga cccgcccgcc gccgcccagg
      481 tgctgaaccc cgagcaggtg ctcgacctcc aggccgcggc cgacgaggtg tacgtggacc
      541 cgggcgtcgt cgactacgcc gtcaatgtcg tgttcgccac gcgggagccc gccgaccacg
      601 gcatgtcgga tctcaccggg atgatcgcct acggcgccag tccccgggcc agcctcggcc
      661 tcgtcgccgc cggccgggcc ctggccctgc tccggggccg ggactacgcc ctgccccagg
      721 acgtgttcga cgtggccccc gatgtgctcc gccaccggct ggtcctctcc tatgaggccc
      781 tggccgacgg cgtcgacgtg aacgacgtcc tgatccgcgt cctctcgacg gttcccgcgg
      841 ccccggtcgc tcccgcccgg gccggatgag cccctcgggc acggccccac cccgggtcgc
      901 gggggcgccg gccgaggagg tcctgcgccg cctgcagatc gacgtcaccc gccggctcga
      961 cgggatgctg cacggtgact tcgagggtct cgtccccggc cggggcagtg atcccggcga
     1021 cacccgggcc tacacgccgg gcgacgacgt ccgccgcatc gactggaacg tcacggcccg
     1081 gatgcccgag gtccacgtgc gcgagaccat cgccgaccgg gaactggcca cgtgggtcct
     1141 gctcgaccgc tcggccagcc tcgacttcgg caccgcgacg tgtgagaagc gggacatggc
     1201 cctggccgcc accgccgcca tcggcttcat caccctgcgc accggcaacc gcctcggcgt
     1261 cgtggtggcg ggcgacggtg acgtgcgcgt cctgcccccc ggcaccgggc gacggcacct
     1321 gctgggcacc ctgcacgccg ccaccctcgc ctcggccgcc gccggtggga gcagtgtcga
     1381 cttggccgcc gccatcgacg ccctggctcg ggcgggacag cgccgccgcg gcctcatggt
     1441 cgtggtgtcg gacttcctgg ccccgggggg ctgggagcgt tcgctgcggg gcctggcctc
     1501 gcgccacgag gtcctcgccg tcgaggtcgt cgacccccgg gagctggccc tgcccgacgt
     1561 cggcgtgatc gagctggtcg atcccgagac cggcgcccag cgcgagatcc acacctcccg
     1621 ggacctgcgg gagcgctacg ccacggccgc cggcgaccag cgccaggaca tcgcccggcg
     1681 ggtccgggcc gccggaagcg accacctggt gctgcgcacg gaccgggact ggctcctcga
     1741 catcgtccgc ttcgtcagcc gccgcaagga acgccgccgc gtcgcggccg ggaccgcgca
     1801 ccattgagct acctcgcccc ccactggctg tggctcctgg ccgtcgtcgg cttcctggcc
     1861 gtcgtctacg ccgtcctgca atggcggcgg cgctccacgt tcaccgtccg cttcaccaac
     1921 ctcgagctcc tcgactccgt cgcccccaag cgcccgggct ggcgccggca cgtcgtggcc
     1981 ggcctgctgc tcgtctcgct gtcgctcctg gtggtggcgc tggcccgacc gacccggccc
     2041 gacgccgcgc cgctgggtcc cgtcctcgtc ctggccatcg acacctcgct gtccatgcag
     2101 gccaccgacg tcgaccccaa ccgcctggag gcggcccagg aggcagccga cggcctggtc
     2161 gaaggtctgt ccgacaacgt ccgggtcggc ctggtcagct tcgacaacgg cgccaccatc
     2221 caggtcacgc cgaccaccga tcacgacgcc gtccgggatg ccatcgacgg cctcagcctg
     2281 gacgagttca ccgccatcgg ggaggccatc ttcgccggtc tcgacgccat cgccatcgcc
     2341 gagcaggacc agcccgaggc ggccgaggat ccggccgccg acgagccgcc acccagccag
     2401 atcgtgctca tgtccgacgg cgagaccacg accggccgcc ccgatgtgga ggccgcccag
     2461 gccgccttcg acgcgggcgt gcccgtgtcg accatcgcct tcggcac
//
LOCUS       DASTFV010000035         5056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_144568, whole genome shotgun sequence.
ACCESSION   DASTFV010000035 DASTFV010000000
VERSION     DASTFV010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144568"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VFP02_00585"
     CDS             <1..858
                     /locus_tag="VFP02_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541545.1"
                     /translation="TTAPGDPVVVWFFGGSAMYGFGQRDNHTLPSELVRRAEADGVAL
                     EAHNFGTPAYVNWQAVSLFSRLLTEREAPDLAVFYDGYNDLSMQFVDGPVTEPSHVLA
                     RVQQAQLEAPPPGQDPTTGADVRDWWADHSATVNLYRRLRDRFDGDDPDVRLTDAEAS
                     AVPADVDADVVEGATDDLLRRGRDLASALGEGYGVDVQFWFQPTLYSKGAVEGEAHLL
                     ERAANRNPAWVPVIDGLRSDFPPEVVDLADSLDGYPDPLFWDTVHTNETGVQLIARRA
                     WAALQQAIG"
     gene            905..1270
                     /locus_tag="VFP02_00590"
     CDS             905..1270
                     /locus_tag="VFP02_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET9541546.1"
                     /translation="MSDFLRSYLTVAIFGAAAIAMVVVALGAGSFLRPTRPQTGKYLT
                     YESGVDPVGTEWSQSQIRYYFFALLFVMFDVEALYIFPWAVRLEVYDVFGLVEMGIFI
                     VILALGLLYAWRKGVLKWV"
     gene            1285..1761
                     /gene="nuoB"
                     /locus_tag="VFP02_00595"
     CDS             1285..1761
                     /gene="nuoB"
                     /locus_tag="VFP02_00595"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HET9541547.1"
                     /translation="MPKPLTKLLNISRKYSLWMYQWGLACCGIEMGAALASPRYDMMR
                     LGVIPFPASPRQADLIVVSGTITDKMAPSVRRLYEQMPDPKYVISMGSCTNCGGPYWD
                     SYSVTKGLDQIIPVDVYIPGCPPRPEALLEAIGLLQAQILDEDPAERWKGEPLVVN"
     gene            1772..2485
                     /locus_tag="VFP02_00600"
     CDS             1772..2485
                     /locus_tag="VFP02_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET9541548.1"
                     /translation="MADEATSDEATPEPEDAVEVDERREALVAALAEGLGDALLATHI
                     DPGREVWARVSLAAWVTAGTHARDRLGLRFFEFLSAIDWMPSPFGRDMDSQEDLEVHG
                     ATEKAPDEQVTGYAGGDTRFQLLARLHNPVEGLGLVLKADLGDDPTALTAPTWVPTFA
                     GAGWHEREAWEMFGIVFEGHPNLTHLYLPGEFEGHPLRKDFPLLSRRVKPWPGIVDVE
                     PMPGEDEAAEDTADDEASE"
     gene            2482..3651
                     /locus_tag="VFP02_00605"
     CDS             2482..3651
                     /locus_tag="VFP02_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D 1"
                     /protein_id="HET9541549.1"
                     /translation="MTVADPQQRAYVASQAADARVNVELETGGMTLNMGPQHPATHGT
                     LRIVVRLDGEQVVEAEPIAGYMHRGYEKLCEVRTYPQVTTLVNRIDWLGSFANEVPFI
                     LAAERLMEVEAPPRAQWIRTILFEMSRIANISLFLGDMGAQLGAITPVFFAFRDRERA
                     LNLIESATGGRFHPNYDRIGGLKDDLPAGWIESCHEVMDRMRDFCDQIEDLLMGNEIF
                     AARCRGVGVIPADVALSYGLSGANLRASGVDWDLRRDVPVGLAFDQLDWQVWTHPDGD
                     SFARFWVRLQEVRESTKMVDQLLDGLPSGPIMAKVPRIIKVPAGEAYVATENPLGAMG
                     YYVISKGDLVPFRVKIRTPSFNNISIVPWVMKGVYVPDVITILGSLYFILGDIDR"
     gene            3651..4697
                     /locus_tag="VFP02_00610"
     CDS             3651..4697
                     /locus_tag="VFP02_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HET9541550.1"
                     /translation="MSSVLSLVSAVDLAYWQETGIKVLVILMVIPAGAIILGYVFLMK
                     MMSHMQSRLGPMEAGPHGVLQLVADGVKFLQKEDIFPEKADRMVFALAPLVVLISTFL
                     LFVVVPVGPDLIVEDLDTGIFFALAVSSISVIGVLMAGWASANKYALLGGLRAAGQLI
                     AYELPLLLAVVGVVIQAGTLSMQGIVFAQADGAIFGIDTIGNPYILTQFVGFFIFLAA
                     VQAELTQPPFDMPVAESEIVGGYQVEYTGFRFLLFFMGEFGTAFAFAGIASVLFLGGW
                     WVPGFDPTDDILNVLGPAVMFGKVMFVAFLIFWVRFTFPRFREDQLQAFAWKVLIPLS
                     LINIAATAVLKVVF"
     gene            4697..>5056
                     /locus_tag="VFP02_00615"
     CDS             4697..>5056
                     /locus_tag="VFP02_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET9541551.1"
                     /translation="MPQLPGLVKGLGVTFRTMLKPAVTVQYPHVKEEPTPRARGVIAL
                     KEENCTVCMLCARECPDWCIYIEGHKYLAPPRREGGKPRQKNELDRFDIDFALCMYCG
                     ICVEVCPFEALFWSPEFE"
BASE COUNT          739 a         1863 c         1618 g          836 t
ORIGIN      
        1 acgacggccc cgggcgaccc ggtcgtggtc tggttcttcg gcggctcggc catgtacggc
       61 ttcggccagc gggacaacca caccctgccc tccgagctgg tccgccgggc ggaggccgac
      121 ggcgtggccc tggaggccca caacttcggc acccccgcct acgtcaactg gcaggcggtg
      181 tcgctgttca gccgcctgct gaccgagcgg gaggcccccg acctggccgt cttctacgac
      241 ggctacaacg acctctccat gcagttcgtg gacggccccg tgaccgagcc cagccacgtg
      301 ctggcccggg tccaacaggc ccagctggag gccccgccgc ctggtcagga cccgacgacc
      361 ggcgccgacg tgcgggactg gtgggcggac cacagcgcca ccgtgaacct ctatcgccgg
      421 ctgcgtgacc gcttcgacgg tgacgacccc gacgtccgcc tcaccgacgc cgaggcgtcg
      481 gccgtgcccg ctgacgtgga cgccgatgtc gtcgaggggg ccaccgacga cctgctccgc
      541 cggggccgcg acctggcgtc ggccctgggc gagggctacg gcgtggacgt ccagttctgg
      601 ttccagccca ccctctactc gaagggggcg gtcgaggggg aggcccacct cctcgaacgg
      661 gccgccaacc gcaacccggc ctgggtcccg gtcatcgacg gcctccggag tgacttcccg
      721 cccgaggtgg tcgacctggc cgacagcctc gacggctacc cggacccgct cttctgggac
      781 accgtccaca ccaacgagac cggcgtccaa ctgatcgccc gccgggcctg ggccgcgctg
      841 caacaggcca ttggctgagc cgttcttggc cgctcgtgag ggcgatcact aacctgcgca
      901 cgcgatgtcg gacttcctcc gcagctacct cacggtggcc attttcggcg cggccgccat
      961 agcgatggtg gtcgtggcgc tgggcgccgg cagcttcctg cgccccaccc ggccccagac
     1021 cgggaagtac ctcacctacg agagcggcgt ggacccggtc ggcaccgagt ggtcccagag
     1081 ccagatccgc tactacttct tcgccctgct cttcgtcatg ttcgacgtgg aggccctcta
     1141 catcttcccg tgggccgtgc gcctcgaggt ctacgacgtc ttcggcctcg tcgagatggg
     1201 catattcatc gtgatcctgg ccctcgggct gctctatgcc tggcgcaagg gagtcctcaa
     1261 gtgggtctag tcgagagcgg caagatgccg aagccgctga ccaagctgct gaacatctcc
     1321 cggaagtact cgctctggat gtaccagtgg ggcctggcct gctgcggcat cgagatgggc
     1381 gcggccctgg cctccccccg ctacgacatg atgcgcctgg gggtcatccc cttcccggcc
     1441 agcccccgcc aggccgacct catcgtcgtc tccggcacca tcaccgacaa gatggccccc
     1501 tcggtccggc gcctctacga gcagatgccc gaccccaagt acgtgatctc gatgggctcc
     1561 tgcaccaact gcggcgggcc gtactgggac tcctactcgg tgaccaaggg cctcgaccag
     1621 atcatcccgg tcgacgtgta catcccgggc tgcccgcccc ggcccgaggc cctgctcgag
     1681 gcgatcggcc tgttgcaggc ccagatcctc gacgaggacc cggccgagcg ctggaaggga
     1741 gagccgcttg tcgtcaactg acggcggttc ggtggccgac gaagcgacca gcgacgaggc
     1801 gacccccgag cccgaggacg cggtcgaggt cgacgagcgg cgcgaggccc tggtggccgc
     1861 actggccgag ggcctgggcg acgccctgct ggccacccac atcgatcccg gccgcgaggt
     1921 ctgggcccgg gtcagcctcg ccgcctgggt gacggcgggg acccacgccc gggaccgtct
     1981 cggcctccgc ttcttcgagt tcctgtcggc catcgactgg atgccctcgc cgttcgggcg
     2041 ggacatggac agccaggagg acctggaggt ccacggcgcc accgagaagg cgcccgacga
     2101 acaggtcacc ggctacgccg gtggcgacac ccgcttccag ctcctggctc ggctccacaa
     2161 cccggtggaa ggcctcggcc tggtgctgaa ggccgacctg ggcgacgatc ccaccgcgct
     2221 gacggccccc acctgggtgc ccaccttcgc cggggcgggc tggcacgagc gcgaggcgtg
     2281 ggagatgttc ggcatcgtct tcgagggcca tcccaacctg acccacctct acctgcccgg
     2341 cgagttcgag ggccacccgc tacgcaagga cttccctctg ctgtcccggc gcgtgaagcc
     2401 ctggcccggc atcgtcgacg tcgagcccat gcccggcgag gacgaagcgg ccgaggacac
     2461 cgccgacgac gaggcgagcg aatgaccgtc gccgatcccc agcagcgggc ctacgtggcc
     2521 agccaggccg ccgacgcccg ggtcaacgtc gagctcgaga ccggcggcat gaccctcaac
     2581 atgggtcccc agcaccccgc cacccacggc acgctgcgca tcgtggtccg cctcgacggc
     2641 gagcaggtgg tcgaggccga gcccatcgcc ggctacatgc accggggcta cgagaagctc
     2701 tgcgaggtcc gcacctaccc gcaggtcacc acgctggtga accgcatcga ctggctgggc
     2761 agcttcgcca acgaggtccc cttcatcctc gccgccgagc ggctgatgga ggtcgaggct
     2821 ccaccccggg cccagtggat ccgcacgatc ctcttcgaga tgtcccgcat cgccaacatc
     2881 tcgctgttcc tcggcgacat gggggcccag ctcggcgcca tcaccccggt cttcttcgcc
     2941 ttccgcgacc gggagcgggc cctgaacctg atcgagtcgg ccaccggtgg ccggttccac
     3001 ccgaactacg accggatcgg tggcctgaag gacgacctgc ccgcgggctg gatcgagtcc
     3061 tgccacgagg tcatggaccg gatgcgggac ttctgtgacc agatcgaaga cctgctcatg
     3121 ggcaacgaga tcttcgccgc ccgctgccgc ggggtcgggg tcatccccgc cgacgtggcc
     3181 ctgtcctacg gcctttccgg cgccaacctc cgggccagcg gcgtggactg ggacctgcgc
     3241 cgcgacgtcc ccgtcggcct ggccttcgac cagctcgact ggcaggtctg gacccatccc
     3301 gacggcgatt cgttcgcccg cttctgggtc cgcctccagg aagtccggga gtcgaccaag
     3361 atggtcgacc agctcctcga cggcctgcct tcggggccga tcatggccaa ggtcccccgc
     3421 atcatcaagg tgccggccgg cgaggcctac gtggccaccg agaaccccct cggggccatg
     3481 ggctactacg tgatcagcaa gggcgacctc gtgccgttcc gggtcaagat ccgcacgccc
     3541 agcttcaaca acatctcgat cgtgccctgg gtgatgaagg gcgtgtacgt gcccgacgtc
     3601 atcaccatcc tgggcagcct ctacttcatc ctgggagaca tcgaccggtg atgtcgtccg
     3661 tgctctccct cgtcagcgcc gtcgacctgg cctactggca ggagaccggg atcaaggtcc
     3721 tggtcatcct catggtcatc ccggccgggg ccatcatcct gggctacgtc ttcctgatga
     3781 agatgatgag ccacatgcag agccgcctcg gccccatgga agcgggtcct cacggcgtgc
     3841 tccagctcgt ggccgacggg gtgaagttcc tccagaagga ggacatcttc cccgagaagg
     3901 ccgaccgcat ggtcttcgcc ctcgcccccc tggtggtgct gatctccacc ttcctgctct
     3961 tcgtggtcgt gcccgtgggt cccgacctca tcgtggagga cctcgacacg ggcatcttct
     4021 tcgccctggc cgtgtcctcc atctcggtca tcggcgtgct catggcgggg tgggcctcgg
     4081 ccaacaagta cgcgctgctc ggaggcctcc gcgccgcggg ccagctcatc gcctacgagc
     4141 tgcccctgct gctggcggtg gtcggcgtgg tcatccaggc cgggaccctg tccatgcagg
     4201 gcatcgtctt cgcccaggcc gacggcgcca tcttcggcat cgacaccatc ggcaacccgt
     4261 acatcctcac ccagttcgtg ggcttcttca tcttcctggc cgcggtgcag gccgagctga
     4321 cccagccccc cttcgacatg ccggtggccg agtccgagat cgtcggcggc taccaggtgg
     4381 agtacaccgg cttccgcttc ctgctcttct tcatgggcga gttcggcacc gccttcgcct
     4441 tcgccgggat cgcctcggtc ctgttcctgg ggggctggtg ggtccccggc ttcgatccga
     4501 ccgacgacat cctcaacgtc ctcggccccg ccgtgatgtt cggcaaggtc atgttcgtgg
     4561 ccttcctcat cttctgggtc cggttcacgt tcccccggtt ccgggaggat cagctccagg
     4621 ccttcgcgtg gaaggtgctg atccccctga gcctcatcaa catcgcggcc accgccgtcc
     4681 tgaaggtggt gttctgatgc cccagctccc cggtctggtc aagggcctcg gtgtgacgtt
     4741 ccgcaccatg ctcaagcccg cggtcaccgt gcagtacccc cacgtcaagg aggagcccac
     4801 gccccgggcc cgcggcgtca tcgcgctgaa ggaggagaac tgcacggtgt gcatgctctg
     4861 tgcccgggag tgcccggact ggtgcatcta catcgagggc cacaagtacc tggccccgcc
     4921 ccggcgggag ggcggcaagc cccgtcagaa gaacgagctg gaccgcttcg acatcgactt
     4981 cgcgctctgc atgtactgcg ggatctgcgt cgaggtctgc ccgttcgagg ccctcttctg
     5041 gagccccgag ttcgag
//
LOCUS       DASTFV010000036         3703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_145475, whole genome shotgun sequence.
ACCESSION   DASTFV010000036 DASTFV010000000
VERSION     DASTFV010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145475"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="VFP02_00620"
     CDS             complement(<1..957)
                     /locus_tag="VFP02_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET9541552.1"
                     /translation="MRWLRRIGLAVAFLLFVGLVASWDGLTGDREDPVPTTTTAAPGR
                     VAAPIAGSAEDPRIACARALPPRRQFGQLLAVSVNGQAPDLEAQHIADLGIGTVFLQS
                     LSPDRPIARIQALKDASAIPPLVAVDEEGGAVQDLRDVLGPFPSEERMAATTDPAGAQ
                     VQVLAHAQSMATLGIDVVFAPVVDVLPGDGRDPLGDDRTFGHDPVLVTRYGQAYVDAF
                     VEAGILPTLKHFPGHGNTTGDSHDGVVSAPPLTEMRDRDLVPYDALLESDVAVMVGHM
                     VVPDVTIVGPSSLSSGIITDLLRDEYAFDGLIFTDSLSMGGVT"
     gene            complement(992..1432)
                     /locus_tag="VFP02_00625"
     CDS             complement(992..1432)
                     /locus_tag="VFP02_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HET9541553.1"
                     /translation="MTAPGFPLPETDWAPTAPFWAAAADERLALPRCEACGRLVWYPA
                     DTCARCGGSDLTWVDLSGRGTLFSWVVVHQQFLPQYEPPYVTGLVAVAEDPSARLATR
                     LLDVDPLAPDLHIDQPVEVVFAPLRFAGVDGEVTAPFWRPISGR"
     gene            complement(1429..2631)
                     /locus_tag="VFP02_00630"
     CDS             complement(1429..2631)
                     /locus_tag="VFP02_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541554.1"
                     /translation="MSDDWGTNVPLDDVGGKVAIVGVGESEHSRASGRTPTEIAATAV
                     ERALADAGLGPEDVDGVMLGPGVPDQLDAAAFHAHFGTSHDLWESTAGGGMRWAATAP
                     VPAAEGLANGDASVIVNSFAVAWATQRGAMTGGPGQLHAEERFKGNLEVPFGWFPQPV
                     YFATIARRHMHQFGTTEAQLGAIAVTCREHANATPGAVMHGRPLSLDDYLASPYLADP
                     FRKEDCCLISDGGAAYVMTTPERARDLDRPSVEVAGVGLGRSHAGTHWAQQADFTSTP
                     QVFAGPAAFAMAGIQPGDVDVATAYDPFTIVSLMQIEDLGFCAKGDGGAFAEAGELRV
                     DGGALPYNPHGGMLSHAYVLGIAHVVEVVRQLRGEAANQVVGAEIGVYGGYTGPEAAT
                     LVLRRSEA"
     gene            complement(2639..>3703)
                     /locus_tag="VFP02_00635"
     CDS             complement(2639..>3703)
                     /locus_tag="VFP02_00635"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET9541555.1"
                     /translation="WWEWAPVFCGTNAGKRGVTLDLGADDGRRLLLDLAQGADAVLEN
                     FSPRVMEQFDLDWPVIHAAAPRAVMVRMPGFGLGGPWRDRTGYAPTMEQLTGLAFVTG
                     WPDLPPIVPRGPCDPLAGQHAAFALLVALEERDRTGEGMLLEVPMVEAALATASGPIV
                     EAEAYGVVQPREGNRGPVAAPQGVHACAEKERWVAVAVTTDEQWAALCAVLGRSDLAD
                     ERALGDHAGRRAAQERLDEALEQWTADQPVADAVEALVGAGVPCAEVVSPGAVDRNPQ
                     LVARGFFEPMTRAVVGVHHIPSLPYRFASRIDRPWHRTPAPLIGEHNDEVLGGELGLG
                     PDELERLRNTSVIGDRPLNA"
BASE COUNT          515 a         1301 c         1421 g          466 t
ORIGIN      
        1 ggtcaccccg cccatggaga gcgagtcggt gaagatcagg ccgtcgaacg cgtactcgtc
       61 ccggagcagg tcggtgatga tcccgctgga caggctggac gggccgacga tggtcacgtc
      121 gggcaccacc atgtggccga ccatcacggc cacatcggac tccagcagcg cgtcgtaggg
      181 gacgaggtcc cgatcccgca tctcggtcag cggcggagcg ctgaccacgc cgtcgtggct
      241 gtcgccggtc gtgttgccgt gacccgggaa gtgcttgagc gtgggcagga tcccggcctc
      301 gacgaaggcg tcgacatagg cctgcccgta ccgggtgacc aggacggggt cgtggccgaa
      361 ggtgcgatcg tcgcccagcg ggtcccggcc gtcgcccggc aggacgtcga ccacgggggc
      421 gaagaccacg tcgatgccga gcgtggccat cgactgggcg tgggccagca cctggacctg
      481 ggccccggcc gggtcggtcg tggccgccat ccgctcctcg gacgggaagg gcccgagcac
      541 gtcgcggagg tcctggacgg ctcccccctc ctcgtccacg gccaccaggg gcgggatggc
      601 cgacgcatcc ttcagggcct ggatccgggc gatgggccgg tcgggggaca ggctctgcag
      661 gaaaacggtc ccgatgccca ggtcggcgat gtgctgggcc tccaggtcag gggcctgccc
      721 gttgaccgac accgccagga gctggccgaa ctgccggcgc gggggcaggg cccgtgcgca
      781 ggcgatgcgg gggtcctcgg cgctcccggc gatgggggcg gcgacccgac cgggggcggc
      841 ggtggtcgtg gtcggcacgg ggtcctcgcg gtcgccggtc agcccgtccc aggacgcgac
      901 gaggccgacg aagagcagga aggcgacggc caacccgatc cgccgcagcc accgcatcct
      961 ctcagcctgc cggacggcgt cgaggttcgg gctagcggcc cgagatcggg cgccagaacg
     1021 gggcggtgac ctcgccgtcg actccggcga agcggagggg ggcgaagacg acctcgaccg
     1081 gctgatcgat gtggaggtcg ggggccagcg ggtccacgtc gagcaggcgg gtggccaggc
     1141 gggcagacgg gtcttcggcc acggcgacga ggccggtgac gtagggaggc tcgtactggg
     1201 gcaggaactg ctggtggacg accacccagg agaacagggt cccccggccg gacaggtcga
     1261 cccaggtgag gtcggacccg ccgcagcggg cacaggtgtc cgccgggtac cagaccaggc
     1321 gcccgcaggc ctcgcaacgg gggagggcca ggcgctcgtc ggcggccgcc gcccagaagg
     1381 gcgcggtcgg ggcccagtcc gtctccggca gggggaatcc gggggcggtc atgcctcact
     1441 ccggcggagg acgagggtgg cggcctcagg ccccgtgtag ccgccgtaga cgccgatctc
     1501 ggcgccgacc acctggttgg cggcctcacc ccgcagctgg cgtacgacct cgaccacgtg
     1561 ggcgatgccc aggacgtagg cgtgggagag catcccgcca tgggggttgt acggcagggc
     1621 gcccccgtcg acccgcaact cgccggcctc ggcgaaggca cccccgtcgc ccttcgcgca
     1681 gaagcccagg tcctcgatct gcatgaggct gacgatcgtg aacgggtcgt aggccgtggc
     1741 cacgtcgacg tcccccggct ggatcccggc catggcgaag gcggcgggcc cggcgaacac
     1801 ctgaggcgtg ctggtgaagt cggcctgttg ggcccagtgg gtgcccgcgt gggaccggcc
     1861 gagcccgacg cctgccacct cgaccgacgg ccggtccagg tcgcgggccc gttcgggcgt
     1921 ggtcatcacg tacgccgccc cgccgtcgga gatgaggcag cagtcctcct tgcggaacgg
     1981 atcggccagg tacggcgagg ccaggtagtc gtccaggctc aggggccggc cgtgcatgac
     2041 ggcgccaggc gtggcgttgg cgtgctcccg gcaggtcacg gcgatggctc cgagctgagc
     2101 ctccgtcgtc ccgaactggt gcatgtggcg gcgggcgatg gtggcgaagt agacgggctg
     2161 ggggaaccag ccgaagggga cctcgaggtt gcccttgaag cgctcttccg cgtggagctg
     2221 ccccggcccg ccggtcatgg caccccgctg ggtcgcccag gccacggcga acgagttgac
     2281 gatgaccgag gcgtcgccgt tggccagtcc ctcggccgcc gggacggggg cggtggccgc
     2341 ccagcgcatc ccgcccccgg cggtcgactc ccacaggtcg tggctggtgc cgaagtgggc
     2401 gtggaaggca gcggcgtcga gctggtcggg cacgcctggg ccgagcatga ccccgtcgac
     2461 gtcctcgggg cccaggccgg cgtcggccag cgcccgttcg acggcggtgg cggcgatctc
     2521 ggtcggcgtg cggcccgacg cccggctgtg ctcggactcg cccaccccga cgatggccac
     2581 cttccccccg acgtcgtcga gggggacgtt cgtgccccag tcgtcgctca tggccggatc
     2641 atgcgttgag gggtcgatcg ccgatcacgg aggtgttgcg tagccgctcc agctcgtcgg
     2701 ggcccaaccc cagctcaccg ccgaggacct cgtcgttgtg ctcaccgatg agcggggccg
     2761 gcgtgcgatg ccagggccgg tcgatcctcg aggcgaagcg gtacggcagg gaagggatgt
     2821 ggtgcacgcc gacgacggcc cgcgtcatcg gctcgaagaa cccccgagcg accagctggg
     2881 ggttgcgatc gacggccccg ggcgacacga cctcggcgca ggggacaccg gcgccgacca
     2941 gcgcctcgac cgcgtcggcc accggttggt cggccgtcca ctgctccagc gcctcgtcca
     3001 accgttcctg ggccgcccgc cgtccggcgt ggtcaccgag ggcgcgctcg tcggcgagat
     3061 cggaccgccc gaggaccgcg cacaacgcgg cccattgctc gtcggtggtc acggccaccg
     3121 cgacccagcg ctccttctcg gcgcacgcat ggacaccctg gggagcggcg acgggtcccc
     3181 gattgccttc tcggggctgg acgacgccgt aggcctcggc ctccacgatc gggccggacg
     3241 cggtagccag ggcggcctcg accatcggga cctcgaggag catcccctca ccggtgcgat
     3301 cccgttcctc cagggccacc agcagggcga aggcggcgtg ctgcccggcc agcgggtcac
     3361 acgggccgcg ggggacgatc ggcggcaggt cgggccagcc cgtgacgaag gccaggccgg
     3421 tgagctgctc catggtcggg gcgtagccgg tgcggtcccg ccacgggccg ccgaggccga
     3481 accccggcat gcggaccatc acggcccgcg gggcggcggc gtggatcacg ggccagtcga
     3541 ggtcgaactg ctccatcacc cggggggaga agttctcgag gacggcgtcg gccccctgcg
     3601 cgaggtcgag caacaggcgg cggccgtcgt cggcccccag gtccagggtc acgccccgct
     3661 tgcccgcgtt cgtcccgcag aacaccgggg cccactccca cca
//
LOCUS       DASTFV010000037         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_146552, whole genome shotgun sequence.
ACCESSION   DASTFV010000037 DASTFV010000000
VERSION     DASTFV010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146552"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFP02_00640"
     CDS             <1..372
                     /locus_tag="VFP02_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET9541556.1"
                     /translation="GLGLTGAELPDQYDQTRWPELRGRLAEVFLTRTRDQWMAVFDGT
                     DACVAPVLDFTEARDHPQMAHRLFPDGGTEVPPVPRLSVTPGQAGASPDWPGCDTDAV
                     LAESGFTGQEIADLRATGAIR"
     gene            402..782
                     /locus_tag="VFP02_00645"
     CDS             402..782
                     /locus_tag="VFP02_00645"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9541557.1"
                     /translation="MDRPIPKSIDEAFAGVNFLAERTPESQDEEFSARVAPYRDGGVF
                     VSHYAGNSEWERHPVGDEIVAVVDGATTMFLLIDGDEVAQAMGPGELVVVPQDTWHRF
                     ETPDGVKVVSVTPQPTDHQIDRPG"
     gene            complement(793..>2568)
                     /locus_tag="VFP02_00650"
     CDS             complement(793..>2568)
                     /locus_tag="VFP02_00650"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9541558.1"
                     /translation="FTNLHHPHRLMSDNQVVEVDRYPDGYYLTDDLTDRCLAWWKELR
                     ASHARKPWFTYLAHPACHAPLHAKAGDIERYRGAYAAGWDEVRARRFARQQDLGIVTE
                     GTELAPRNTEEGDDVRPWDELAEPEKALFARHMEVYAGMVDGLDQSIGRLLDGLAELD
                     ELDNTIVVFTSDNGASREGEEAGTSGYFVHLLGQPDVEADLPRMDLLGGPQTMPHYPR
                     GWAMASNTPFRLYKINTHAGGHSVPFILSWPDHLAAGGELRRQYAHVVDLLPTLLDLT
                     GVEAPTERHGRPLIPMAGTTFAATLADAEHPPTRTEQVYEMVGHRGFYRDGWEVVTRH
                     VPMTPIDDAEWQLYDLTTDPTELRDLAAERPEKVAELAAAWEAAAHAEQIYPLDEGSN
                     LKFVQRPERSRVFSEPVTIYPGTPTLERWRSLELINMRDFTVTASVDLAEGDRGWLVA
                     HGDQGGGYGLYVDDGRLWWLHNDGKGVVRRLEGGPVGTGAVQLAAIVSAPGGFTWDVT
                     LTVDGTSRGSDTGYRMLFPMSPFEGIDVGRDRRSPVSWEIYEADGPFPFTGRLHHVRY
                     EPGALAPDSAWRWKDVLKEMGAKFE"
BASE COUNT          339 a          904 c          936 g          389 t
ORIGIN      
        1 ggcctcggcc tcaccggtgc ggaactgccc gaccagtacg accagacccg ctggcccgag
       61 ctccggggcc ggctggcgga ggtgttcctg acccggaccc gggaccagtg gatggcggtg
      121 ttcgacggca ccgatgcctg cgtggctccc gtcctcgact tcaccgaggc ccgcgaccac
      181 ccccagatgg cccatcgcct gttccccgac ggcggcaccg aggtcccgcc ggtgccccga
      241 ctgtcggtca cccccggcca ggcgggggcg tcgccggact ggcccggctg cgacaccgat
      301 gccgtcctgg ccgagtccgg cttcacgggc caggagatcg ccgacctccg ggccaccggc
      361 gccatccgct gagctcgacc gtcgtcggta gcctcgggtc catggaccgc cccatcccga
      421 agtcgatcga cgaggcgttc gccggtgtga acttcctggc cgagcggacc ccggagagcc
      481 aggacgagga gttctcggcc cgggtcgccc cgtaccgtga cgggggcgtc ttcgtctccc
      541 actacgccgg gaactccgaa tgggagcgcc atccggtcgg ggacgagatc gtggccgtcg
      601 tcgacggggc gacgaccatg ttcctgctca tcgacggcga cgaggtcgcc caggccatgg
      661 gtccgggcga gctcgtggtc gtgccccagg acacctggca ccggttcgag acgccggatg
      721 gggtgaaggt cgtgtccgtg acgccccagc cgacggacca tcagatcgac cggcccggct
      781 gacgtcggcg ggctactcga acttggcgcc catctccttg agcacgtcct tccagcgcca
      841 ggcggagtcg ggggcgagcg cgccgggctc gtagcggacg tggtgcagcc tgccggtgaa
      901 ggggaagggc ccgtcggcct cgtagatctc ccaggacacg ggggagcgcc ggtcccggcc
      961 cacgtcgatg ccctcgaacg gcgacatcgg gaacagcatg cggtagccgg tgtccgagcc
     1021 ccgtgacgtg ccgtcgacgg tcaaggtcac gtcccaggtg aagccgcccg gggcgctgac
     1081 gatcgcggcc agttggacgg cgccggtccc gaccggacct ccttccagcc gtcgcaccac
     1141 tcccttaccg tcgttgtgga gccaccacag ccgcccgtcg tcgacgtaca ggccgtagcc
     1201 cccgccttgg tcgccgtggg cgacgagcca accccggtcg ccctcggcca ggtcgaccga
     1261 cgccgtgacg gtgaagtccc gcatgttgat cagctcgagg gagcgccagc gctcgagggt
     1321 cggcgtgccc gggtagatcg tcaccggttc gctgaacacc cgcgaccgct ccgggcgctg
     1381 gacgaacttg aggttgctgc cctcgtccag gggatagatc tgctcggcgt gggccgccgc
     1441 ctcccacgcc gcggccagct ccgccacctt ctccgggcgt tcggcggcca ggtcccggag
     1501 ctcggtcggg tccgtcgtca ggtcgtagag ctgccactcg gcatcgtcga tcggcgtcat
     1561 cggcacgtgg cgggtgacca cctcccagcc gtcccggtag aagccccggt ggccgaccat
     1621 ctcgtagacc tgctcggtcc gcgtcggcgg gtgctcggcg tcggccagcg tggccgcgaa
     1681 cgtggtcccg gccatcggga tgagcgggcg gccgtggcgc tcggtcggcg cctccacccc
     1741 ggtcaggtcc aggagcgtgg gcaggaggtc gacgacgtgg gcgtactggc gccgcagctc
     1801 gcccccggcg gccaggtgat cgggccagga caggatgaag ggcaccgagt ggcccccggc
     1861 gtgggtgttg atcttgtaga gccggaacgg tgtgttcgaa gccatggccc agccccgggg
     1921 gtagtgcggc atggtctggg gaccgcccag gaggtccatc cgggggaggt cggcttcgac
     1981 gtcgggctgg cccaggaggt ggacgaagta gccactggtc ccggcctcct caccctcccg
     2041 ggaggcgccg ttgtcggacg tgaagacgac gatcgtgttg tccagctcgt ccagctcggc
     2101 cagtccgtcg agcagccgcc cgatgctctg gtcgaggccg tcgaccatgc ccgcgtagac
     2161 ctccatgtgc cgggcgaaca gggccttctc gggctcggcc agctcgtccc aggggcggac
     2221 gtcgtcgccc tcctcggtgt tgcggggcgc cagctcggtg ccttcggtga cgatccccag
     2281 gtcctgctga cgggcgaagc gccgggcccg gacctcgtcc cagcccgcgg cgtacgcacc
     2341 ccggtagcgc tcgatgtcgc cggccttcgc gtggagcggg gcgtggcagg cggggtgggc
     2401 caggtaggtg aaccagggct tgcgggcatg gctggcccgc agctccttcc accaggcgag
     2461 gcagcggtcc gtgaggtcgt cggtcaggta gtacccgtcg gggtagcggt cgacctcgac
     2521 cacctggttg tcgctcatca ggcggtgggg gtggtgcagg ttcgtgaa
//
LOCUS       DASTFV010000038         4280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_147678, whole genome shotgun sequence.
ACCESSION   DASTFV010000038 DASTFV010000000
VERSION     DASTFV010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147678"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1249
                     /gene="rffA"
                     /locus_tag="VFP02_00655"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             107..1249
                     /gene="rffA"
                     /locus_tag="VFP02_00655"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF008687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HET9541559.1"
                     /translation="MPDSEIRFNQPTLEGREVEYIQQAVHGGHTSMNGPFAKRVTALL
                     EEELDAAGVLLTTSCTDALEMAAMLLALEPGDTVIVPSFTFVSSALAFIREGAKIVFA
                     DIERETLGIDPHHVAELIDDSTRAVVAVHYAGVACDVPGLRAVLADHPRIDLIEDNAH
                     GLFGRFDGQPLGGFGRMSAVSFHETKSFTCGEGGALVLNDPADVERANILYEKGTDRR
                     AFEQGTVDKYTWRDTGSSFGMSDILAAYLLGQLEARDAILAKRRAVVERYQERLAPDA
                     AALGFEVPVMPGARDYAYHLYYVLLEDADTRTRVQGALRAAGVQASFHFVPLHDSEGG
                     RRFRAYDSDCPVTDEITSRLLRLPVHNALTPDDADRISDAFLSALA"
     gene            complement(1318..3138)
                     /locus_tag="VFP02_00660"
     CDS             complement(1318..3138)
                     /locus_tag="VFP02_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541560.1"
                     /translation="MKVRVTTAKGTYWVEEDEIDAIDDDTAADDEVPSGDQQDVDHPD
                     PEAPGGMFAWLGDRFQTSPRVAEWVVGVLVAMAAVAVTAYMARDMWFTSDEWEYLANR
                     TAFDLGDLTRPVGGHWTTWSVLLLRGLYKTFGVDFWPWFYIPRLIGHTVLVTFIWRVM
                     RRRGADPLVGIFAYAILLVLGASGYQRALQVGNWAVYAALIICALVISRRPAKPTTRD
                     RVIVAVALMIAVLGNGYAVAVIGGIILTLLVARRLVAWIPSLIPAVLAYGIWWLNYRD
                     DIKPKPELKPSKLLDIPWGAFRVVRTAVEAATGFPTWLAAIAVLGLLAWIVVLAVRRR
                     FDLFDWIILATLAIGLCLLVVQRISIDDEAATRLRYGYSVSIMLAVALVPHIRLSKTL
                     LVRGLFVLAGCGMIAANLAQMKDAINVREEVAQEARVLSVAAAEMVRAGEPVVAGPST
                     LAHGLETNELIHLVDDGYDPASLPEDQIDRDLIEADARGALRMNLIDRNRPRGGHLPE
                     AGTPPTTAADVDAEGCVVVAQGDTVTATVADAGLITFDKTRPQSLALTWTDEFGEGRR
                     FFDDPDIRQGAVELATPATDADLTLESPVGDLTVCGFSAP"
     gene            complement(3135..>4280)
                     /locus_tag="VFP02_00665"
     CDS             complement(3135..>4280)
                     /locus_tag="VFP02_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541561.1"
                     /translation="AYLFIAIGTRQFGTSDFSGVAQLWTIWFFGSSVLTFPIQHWVIQ
                     RLRTDGHGGAVRVVLPRLMLFALGVGLAVVAVTFLVRGDMYGRDDVAYPLVAGGITVG
                     AAAMGLLRGGLAGHSRFLATATALAGENVIRFACGLAVVAAGATVGWYGAAILSGSLV
                     ILAWPSALRFDGPPVPGVSPVAFLGNVAGGTVIAQSVLTAGPVVLSLAGGTRDEVTSL
                     FTALALFRAPYIVALGLAIRMTATLTDLVAAGRSDDLRRIRRRMIAAGVALMAVGGIG
                     AALIGPDLLRLIFGDEVDLTRAACAGLAVGSLAALATLGLTLVQMTDGAGRHVLRTWA
                     TALAVGVVVMVALPLEPLPRVVAAFLASELTAFVLMALLDGSGERSR"
BASE COUNT          679 a         1481 c         1555 g          565 t
ORIGIN      
        1 gaggtggccc tcgacgacgg cacgcgggcc cggctggtcg cctggttcgc cgatgccaac
       61 gccgacctgg ctgagcagct gggtcgcccg atcgaccggt gggcccatgc ctgactccga
      121 gatccgcttc aaccaaccca ccctggaagg ccgggaggtc gagtacatcc agcaggcggt
      181 gcacggcggc cacacgtcca tgaacgggcc gttcgccaaa cgggtgacgg ccctgctgga
      241 ggaagagctg gatgccgccg gtgtcctgtt gaccacctcc tgcaccgacg cgttggagat
      301 ggcagccatg ctgctggcgc tcgagccggg cgacacggtg atcgtgccct cgttcacgtt
      361 cgtgtccagc gccttggcct tcatccgcga gggggccaag atcgtcttcg ccgacatcga
      421 gcgggagacc ctcggcatcg acccccacca cgtggccgag ctcatcgacg actcgacccg
      481 ggccgtcgtg gccgtccact acgccggagt ggcgtgcgac gtgccgggcc tgcgggccgt
      541 gctcgccgat cacccccgca tcgacctgat cgaggacaac gcccacgggc tcttcgggcg
      601 cttcgacggt cagcccctgg gcggcttcgg ccggatgtcg gcggtcagct tccacgagac
      661 caagagcttc acgtgcggcg agggtggggc cctggtcctc aacgacccgg ccgacgtcga
      721 gcgggccaac atcctctacg agaagggcac cgaccggcgc gccttcgagc agggcacggt
      781 cgacaagtac acctggcggg acacggggtc gtccttcggg atgtccgaca tcctggccgc
      841 ctacctcctg ggccagctcg aggcccggga cgcgatcctg gccaagcgtc gggcggtggt
      901 ggagcgctac caggagcggc tggcccccga cgccgcggcg ctgggcttcg aggtcccggt
      961 catgcctggc gctcgggact acgcctacca cctgtactac gtgctcctgg aggacgccga
     1021 cacccgcacc cgggtgcagg gggcgcttcg ggccgcgggg gtgcaggcct cgttccactt
     1081 cgtcccactg cacgactcgg agggcggccg ccgcttccgg gcctacgaca gcgactgccc
     1141 cgtgaccgac gagatcacca gccgcctgct ccgcctcccg gtccacaacg ccctcacccc
     1201 cgacgacgcc gaccgcatca gcgacgcctt cctgtccgcg ctcgcctgat ccctcgttct
     1261 ggcgcctgat cgcggtgcta tggcacccga atcaggcagc agttcggcag ggtcggatca
     1321 gggtgctgag aagccgcaga cggtgagatc gccgacgggg gactcgaggg tgaggtcggc
     1381 gtcggtggcc ggggtggcca gctcgacggc gccctgacgg atgtcggggt cgtcgaagaa
     1441 gcggcggccc tcgccgaact cgtcggtcca ggtcagggcc agggactggg gtcgggtctt
     1501 gtcgaaggtg atcagcccgg cgtcggcgac ggtggccgtg acggtgtcac cctgcgcgac
     1561 gacgacgcag ccctcggcgt ccacgtcggc ggcggtcgtc ggcggcgttc ccgcctcggg
     1621 caggtggccg ccgcggggtc ggttgcggtc gatcaggttc atgcgcaacg cgccccgggc
     1681 gtcagcctcg atcagatccc ggtcgatctg gtcctcgggc agcgacgcgg ggtcgtaacc
     1741 gtcgtcgacc aggtggatca gctcgttggt ctcgagcccg tgggcgaggg tcgacggtcc
     1801 cgccaccacg ggctcgccgg cccggaccat ctcggccgcg gctacggaga ggacgcgggc
     1861 ctcctgggcc acctcctcgc gcacgttgat ggcgtccttc atctgcgcga ggttggccgc
     1921 gatcatcccg cagccggcca ggacgaagag cccccgcacg agcagcgtct tggacagccg
     1981 aatgtggggc accaaggcga ccgccagcat gatgctgacc gagtagccgt agcgcaggcg
     2041 ggtcgcggcc tcgtcgtcga tggagatgcg ctggacgacg agcaggcaca ggccgatggc
     2101 cagcgtggcg aggatgatcc agtcgaagag gtcgaaccgg cggcggacgg ccagcaccac
     2161 gatccaggcc aagaggccca agacggcgat ggcggccagc caggtcggga acccggtggc
     2221 ggcctcgacg gcggtgcgca ccacccggaa ggcgccccac gggatgtcga ggagcttgct
     2281 cggcttcagc tcgggcttcg gcttgatgtc gtcccggtag ttcagccacc agatcccgta
     2341 ggcgaggaca gccgggatca gcgacgggat ccaggcgacc aggcgccggg ccaccagcag
     2401 ggtgaggatg atgccgccga tgacggccac ggcgtagccg ttgccgagga cggcgatcat
     2461 cagggccacg gcgacgatca cccggtcccg cgtcgtcggc ttcgcgggtc gacggctgat
     2521 gacgagggcg cagatgatca gggcggcgta caccgcccag ttcccgacct gcagggctcg
     2581 ctggtagccg ctggcaccga ggacgaggag gatggcgtag gcgaagatgc ccaccagggg
     2641 gtcggccccc cgacggcgca tgacccgcca gatgaacgtc accagcaccg tgtggccgat
     2701 gaggcggggg atgtagaacc acggccagaa gtcgacgccg aaggtcttgt agaggccgcg
     2761 gagcagcagg acgctccagg tcgtccagtg gcctccgaca gggcgggtca ggtccccgag
     2821 gtcgaaggcg gtgcggttgg ccaggtactc ccactcgtcg ctggtgaacc acatgtcgcg
     2881 ggccatgtag gcggtgacag cgacggcggc catggccacg aggaccccga ccacccattc
     2941 cgcaacccgc gggctggtct ggaaccggtc gcccagccag gcgaacatcc cgccgggggc
     3001 ctcgggatcg gggtggtcga cgtcctgctg gtcgccgctg gggacctcgt cgtcggcggc
     3061 ggtgtcgtcg tcgatggcgt cgatctcgtc ctcttcgacc cagtaggtcc ccttcgcggt
     3121 cgtgacccgt accttcatcg gctgcgttct ccggatccgt cgagcagcgc catgagcaca
     3181 aacgctgtca gctcactggc gaggaaggcc gcgacgaccc ggggaagggg ttcgaggggc
     3241 agcgccacca taacgacgac cccgacggcc aacgcggtcg cccacgtccg caacacgtgt
     3301 cgaccggctc cgtccgtcat ctgcaccagg gtcaggccca gcgtggccag ggcggcgaga
     3361 ctgccgaccg ccaggccggc acaggcggca cgggtgagat cgacctcgtc cccgaagatc
     3421 aggcggagca ggtcgggccc gatgagggcc gccccgatcc cgccgacggc catgagggcc
     3481 acccccgcgg cgatcatccg ccgccggatg cggcgcaggt cgtcggaccg cccggccgcc
     3541 accaggtccg tcagcgtcgc cgtcatgcgg atggcgaggc ccagggccac gatgtacggc
     3601 gcgcggaaga gggccagagc cgtgaacagg ctggtgacct cgtcccgggt cccgccggcg
     3661 aggctgagca cgacgggtcc cgcggtcagg acggattgtg cgatgaccgt gccgccggcg
     3721 acgttgccga ggaaggccac aggggagacc ccaggcaccg gcggcccgtc gaagcgcagg
     3781 gccgagggcc aggccaggat gaccagcgac ccgctgagga tggcggcccc gtaccagccc
     3841 acggttgccc cggcggcgac caccgccagg ccacaggcga agcggatgac gttctcgccg
     3901 gccagcgcgg tggcggtggc caggaaccgt gaatggccgg ccaaccctcc ccggagcagg
     3961 cccatggccg ccgcgccgac ggtgatgccg ccggcgacca ggggatacgc gacgtcgtcc
     4021 cgcccgtaca tgtcgccccg gaccaggaag gtgacggcga cgacggccag accgacgccg
     4081 agggcgaaca gcatcaggcg ggggagcacg acccggaccg ccccgccgtg gccgtcggtg
     4141 cgcaggcgtt ggatgaccca gtgctggatc gggaaggtca ggaccgacga gccgaagaac
     4201 cagatcgtcc acagctgggc gacgccggag aagtcgctgg tgccgaactg ccgggtcccg
     4261 atggcgatga acaggtaggc
//
LOCUS       DASTFV010000039         4792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_148454, whole genome shotgun sequence.
ACCESSION   DASTFV010000039 DASTFV010000000
VERSION     DASTFV010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148454"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VFP02_00670"
     CDS             <1..601
                     /locus_tag="VFP02_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9541562.1"
                     /translation="KAATIEHLQLTAIALGVGLVLSIALATIAIRWQKAYAPIAGFAG
                     VLYAIPSLALFAILFPITGLTVLTAEIALVGYTLLILIRNIVAGVQGVPDAVKEAADG
                     MGYRPLHRFFAVDLRLATPAIVAGIRIAAVTIIGLVTVTFVIGFGGYGSLINDGLSRD
                     FNTPVVVGAILSIALALAIDIILLLVERLLTPWNRAARR"
     gene            598..1347
                     /locus_tag="VFP02_00675"
     CDS             598..1347
                     /locus_tag="VFP02_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9541563.1"
                     /translation="MSALNEFTTFVTTSENYWGERGILIRTWDHLRLSGFALLVSSLL
                     SLPPAVYLGHIKRGGVSAVWVVNMGRAIPSFALIVLIFPISLRYGFGLGFWPTAFALV
                     LLGIPPIFTNAFTGVRDVDAGTVEAARGMGMQGREVLWGVEIPAALPLIITGLRVSAV
                     QVVATATLGAYVGFGGLGAFIVEGFAIQDDGKLLTGAILVALVVFVVELVFGWLQRRL
                     TPWVRADLLSKRDPRSFDIVDADQPTAPARL"
     gene            1412..2341
                     /locus_tag="VFP02_00680"
     CDS             1412..2341
                     /locus_tag="VFP02_00680"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9541564.1"
                     /translation="MLAVLALLAGACGDDSGDDSASDETTATGDTASDASVPPDGPTI
                     TIGTQDFGESAILSQIYGQALINAGYSVEYQELGGFRDLEVPAFESGDINFAPEYVAS
                     MLEFLNEFAGEATADVDATKALLDEQLTEIGLQALEPSPAQNRNTFVVTPETSEQLGL
                     TSISDLTEDLTLGAPQDCEENAFCLPGLQDVYGIDLSGGFTPLDPGVLPDALDNGEID
                     VAVMFSTDGVIGENGYIQLEDDQELFNAENIVPVVTDEVVDAYGADFESLVNEVSAAL
                     TTELLTAANARFQIDAEDADVIAADFLADNGLG"
     gene            complement(2409..2972)
                     /locus_tag="VFP02_00685"
     CDS             complement(2409..2972)
                     /locus_tag="VFP02_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9541565.1"
                     /translation="MATTDTFTRMDESTAEQWAEIGKQTFEHRDRVADRVLDMLRSLE
                     AITDGFAVDQLEHSVQTATRAERAGADPEVVVAALCHDIGKAVSVPNHPEIAAAILKP
                     YVRPEVHDMIRVHQDFQGRHYYAHFGGDPDAREQHRAALDPEVFALAERFADEWDQVA
                     FDPGYDSEPLEHFEPLVREVFATAKML"
     gene            complement(2997..4028)
                     /gene="ispH"
                     /locus_tag="VFP02_00690"
     CDS             complement(2997..4028)
                     /gene="ispH"
                     /locus_tag="VFP02_00690"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET9541566.1"
                     /translation="MAVDRVLLCAPRGFCAGVEMAIKALAWMVRTFEPPVYCYHEIVH
                     NQRVVDRFRDLGVVFVDDLEAVPQGSPIMLSAHGSAPEVVETARRQGGYVVDAVCPLV
                     TKVHHEVRVRAGKGYRIVYVGHEGHEEAVGTMAVAPDAIDRVERPEDIAALADRGQPV
                     ALLAQTTLSHRDWAGVLDAANERWPDLWQPGRSDLCFATTNRQSGLLQIADRCDAIVV
                     IGSANSSNTRALEQLARDAGCPRVFRVNDPDELPDDITGTVGVTAGASAPEDLVDAVI
                     ARLAPTEGTEEVWFTDEDEYFPPPRNLRDLLAAVDEAATVTLGGSLSARPATNDRSID
                     AADVLAALG"
     gene            4072..>4792
                     /locus_tag="VFP02_00695"
     CDS             4072..>4792
                     /locus_tag="VFP02_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9541567.1"
                     /translation="MSAPLVLAVAPGSPADRAGLRPGDELRALNGEAPRDIIRYQILV
                     DDPDLEIDVSRGGLDLTIGVQKGAGEPLGVEVESAVFDAVRTCDNHCEFCFIHQLPPG
                     MRSSLYQRDDDYRLSFLYGNFTTLTRFTEMDLERVITERLSPLYVSIHATDPDVRSEM
                     LRNRRGATSLRWLRALLDAGVLVHGQVVVCPGVNDGAVLEDTLVGIADQYPELATVAV
                     VPLGISRHGREGRLRPHTVAEA"
BASE COUNT          696 a         1652 c         1653 g          791 t
ORIGIN      
        1 caaggccgcc accatcgagc acctccagct caccgccatc gccctcggcg tcgggctggt
       61 cctctcgatc gccctggcca cgatcgccat ccgctggcag aaggcctacg ccccgatcgc
      121 cgggttcgcc ggggtcctct atgccatccc cagcctggcc ctgttcgcca tcctgttccc
      181 gatcacaggc ctgaccgtgc tgacggccga gatcgccctc gtcggctaca cattgctgat
      241 cctcatccgc aacatcgtcg cgggcgtgca gggcgtgccc gacgcggtca aggaggcggc
      301 cgacggcatg ggctaccggc cgctgcaccg gttcttcgcc gtcgacctcc ggctggccac
      361 cccggccatc gtggccggca tccgcatcgc ggcggtgacc atcatcgggc tggtgacggt
      421 gaccttcgtc atcggcttcg ggggctacgg ctcgctcatc aacgacgggc tgtcccggga
      481 cttcaacacc ccggtggtcg tgggggcgat cctgtcgatc gccctggccc tggccataga
      541 catcatcctc ctgctcgtcg agcgcctgct cacgccgtgg aaccgggcgg cgcggcgatg
      601 agcgcgctga acgagttcac gaccttcgtg acgacctcgg agaactactg gggcgagcgg
      661 gggatcctca tccgcacctg ggaccacctc cggctgtccg gcttcgccct cctcgtgtcg
      721 tcgctgctgt ccctgccccc ggcggtctac ctgggccaca tcaagcgggg tggggtgagc
      781 gcggtctggg tcgtcaacat gggacgggcc atcccgtcct tcgccctcat cgtgctcatc
      841 ttcccgatct cgctgcgcta cgggttcggg ctggggttct ggccgacggc gttcgccctg
      901 gtgctgctgg gcatcccgcc gatcttcacc aacgccttca ctggtgtccg cgacgtcgac
      961 gccgggaccg tcgaggccgc ccgggggatg ggcatgcagg gccgcgaggt gctgtggggc
     1021 gtggagatcc ccgcggccct gcccctgatc atcaccggcc tgcgggtgtc ggccgtgcag
     1081 gtggtggcca cggccacgct gggggcctac gtgggcttcg gtggcctcgg cgccttcatc
     1141 gtcgagggct tcgccatcca ggacgacggc aagctcctga ccggggccat cctcgtggcc
     1201 ctcgtggtct tcgtggtaga actggtgttc ggctggctcc agcgccgtct gaccccctgg
     1261 gtccgagccg acctgctatc caagcgggac ccgagatcct tcgacatcgt cgacgccgac
     1321 caaccgacgg ccccggcgcg gctgtgacag atcgcagatt gcgaaccaaa gggagaagtc
     1381 aatgaccagg aagatcagag ggttgtgggc ggtgctggcc gtcttggcgt tgctcgccgg
     1441 tgcgtgtggc gatgacagcg gcgacgacag cgcgtcggac gagaccacgg cgaccggcga
     1501 cacggccagc gacgcctccg taccccccga cggccccacc atcaccatcg ggacccagga
     1561 cttcggcgag agcgccatcc tgtcccagat ctacggccag gcgctcatca atgccggcta
     1621 ctccgtggag taccaggagc tgggtggctt ccgtgacctg gaggtccccg ccttcgagtc
     1681 cggtgacatc aacttcgccc cggagtacgt ggcctcgatg ctcgagttcc tgaacgagtt
     1741 cgccggagag gcgacagcag acgtcgacgc gaccaaggct ttgcttgatg agcagctgac
     1801 cgagattggt ctccaggcgc ttgagccgag cccggcgcag aaccgcaaca ccttcgttgt
     1861 cacgcctgag acctcagagc agctgggact tacatccatc agcgatctga ccgaggatct
     1921 gacactcggg gcgccgcaag attgtgaaga aaatgcgttc tgcctgccgg ggttgcaaga
     1981 cgtgtatggg atcgacctca gtggcggctt cacgccgcta gatccgggcg tccttccaga
     2041 cgcgctagac aacggcgaga tcgatgtggc tgtcatgttc tcgacagacg gagtaatcgg
     2101 cgagaacggc tacatccagc ttgaggacga tcaggagctg ttcaacgcag agaacatcgt
     2161 gccagttgtc accgacgagg ttgtcgacgc ctacggagca gatttcgaga gtttggtgaa
     2221 cgaggtcagc gcggcgctca ccaccgaact cctcacagca gccaacgcac ggttccagat
     2281 cgacgccgag gacgccgacg tcatcgccgc cgacttcctc gccgacaacg gcctgggcta
     2341 gtcgccggaa gcgcttctgg cgcctgattt cgggcctctg gccccgaaat caggcgccag
     2401 ttcgcgttct agagcatctt ggcggtggcg aagacctcgc ggaccagcgg ctcgaagtgc
     2461 tcgaggggct cgctgtcgta gcccgggtcg aaggcgacct ggtcccactc gtcggcgaag
     2521 cgctcggcca gggcgaagac ctcggggtcc agcgcggcgc ggtgctgctc ccgggcgtcg
     2581 gggtcgcccc cgaagtgggc gtagtagtgc cggccctgga agtcctggtg gacccggatc
     2641 atgtcgtgga cctcggggcg cacgtagggc ttgaggatgg cggcggcgat ctcggggtgg
     2701 ttgggcacgc tgaccgcctt gccgatgtcg tggcacaggg cggcgacgac gacctcggga
     2761 tcggcccccg cccgctcggc ccgggtggcc gtctggaccg agtgctccag ctgatccacg
     2821 gcgaagccgt cggtgatggc ctccagcgag cggagcatgt ccagcacccg gtcggcgacc
     2881 cggtcgcggt gctcgaaggt ctgcttgccg atctcggccc actgctcggc cgtcgactcg
     2941 tccatgcggg tgaaggtgtc ggtcgtcgcc atgaccaaca gtctgccagc cccggctcag
     3001 cccagtgcgg ccagcacgtc ggcggcgtcg atgctgcggt cgttggtggc ggggcgggcg
     3061 gacagggagc cgcccagggt gaccgtggcc gcctcgtcca ccgccgccaa caggtcccgc
     3121 aggttgcggg gcggggggaa gtactcgtcc tcgtcggtga accagacctc ctcggtgccc
     3181 tcggtcgggg cgaggcgggc gatcacggcg tcgaccaggt cttcgggggc cgacgccccg
     3241 gcggtgaccc cgaccgtgcc cgtgatgtcg tccggcagct cgtcggggtc gttgacccgg
     3301 aagacccggg ggcagccggc gtcgcgggcc agctgctcga gggcccgggt gttggaggag
     3361 ttggccgaac cgatgaccac gatggcgtcg cagcggtcgg cgatctggag caggccggac
     3421 tggcgattgg tggtggcgaa gcacaggtcg ctgcgaccgg gttgccacag gtcgggccag
     3481 cgctcgttgg cggcgtcgag gacgccggcc cagtcccggt gcgagagcgt ggtctgggcc
     3541 aggagggcga cgggttggcc gcggtcggcc agggcggcga tgtcttcggg gcgctcgacc
     3601 cggtcgatgg cgtcaggggc gacggccatg gtgcccaccg cctcctcgtg gccctcgtgg
     3661 cccacgtaga cgatgcgata gcccttgccc gcccggaccc gcacctcgtg gtggaccttg
     3721 gtcaccaggg ggcagacggc gtcgaccacg tacccgcctt ggcggcgggc ggtctcgacc
     3781 acctcggggg ccgatccgtg ggccgacagc atgatgggtg agccctgggg gaccgcctcc
     3841 aggtcgtcca cgaagaccac cccgaggtcc cggaagcggt ccaccacccg ctggttgtgg
     3901 acgatctcgt ggtagcagta gacgggcggc tcgaaggtcc gcaccatcca ggccagcgcc
     3961 ttgatggcca tctccacgcc cgcgcagaac ccccgcggcg cacagagcag gacccggtcg
     4021 acggccatcg atccgagggt atcggtgccc ctcgctccgt atccttgacg ggtgtctgct
     4081 ccactcgttc ttgctgtcgc gcccggttcg ccggccgacc gggcggggtt gcgccccggt
     4141 gacgagctgc gggccctgaa cggcgaggcc ccccgcgaca tcatccgcta ccagatcctg
     4201 gtcgacgacc cggacctgga gatcgacgtg tcccggggcg gcctggacct caccatcggg
     4261 gtccagaagg gggcggggga gccgttgggc gtcgaggtcg agtcggccgt cttcgacgcc
     4321 gtgcgcacct gtgacaacca ctgtgagttc tgcttcatcc accagctccc accggggatg
     4381 cgctccagcc tctatcagcg cgacgacgac taccgcctgt ccttcctcta cgggaacttc
     4441 accacgctga cccggttcac cgagatggat ctcgagcggg tgatcaccga gcgcctgtcg
     4501 ccgctctacg tctccatcca cgccaccgat ccggacgtgc ggtccgagat gctgcgcaac
     4561 cggcgcggcg ccaccagtct gcgctggctg cgggccctcc tcgacgccgg cgtgctggtc
     4621 cacggccagg tcgtggtctg ccccggggtc aacgacggcg ccgtcctcga ggacacgctg
     4681 gtcgggatcg ccgaccagta ccccgagctg gcgaccgtcg ccgtggttcc cctcggcatc
     4741 agccgtcacg ggcgcgaggg acgtctgcga ccccacaccg tcgcggaggc cg
//
LOCUS       DASTFV010000040         3546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_148456, whole genome shotgun sequence.
ACCESSION   DASTFV010000040 DASTFV010000000
VERSION     DASTFV010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148456"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1239
                     /locus_tag="VFP02_00700"
     CDS             163..1239
                     /locus_tag="VFP02_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9541568.1"
                     /translation="MEGPPADAVKLLRFTPFSVIRAVGMVAGTLLVLAVFRAATRPLG
                     WLVVAVVGAALLGPFVALLQRALPRGLAVVVALLVAAAAFGAVAYVVVDDLSRELNRV
                     QLISRRAAGEVERSERYGEFAREIHLRDRVNAFVEELPARLRGGTDVAAIQSAASRGV
                     SFLITFVLLLFMLATGPKFIEGGLRQIDDDVRRARARTLLLESYGRFWRYVAATLGRA
                     VLAGFFTYFVVQVADLPAPVLLAVWVGAWSIVPAVGIVVGSLAVGLVAIPHSFSLAGW
                     LMVLFIGYQVFDALVLEKRIDRSILHLGSFGTFVAAALGLEAYGLGGLIVATLLAIFL
                     AAMVRTITSRPTPVAVDEVLAGAG"
     gene            1252..1794
                     /locus_tag="VFP02_00705"
     CDS             1252..1794
                     /locus_tag="VFP02_00705"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET9541569.1"
                     /translation="MTIYTKKGDDGTTGLLYGGRVAKDAAAPTAYGEVDEAQAFLGVV
                     RAQADRELAGIVLGLQRDLWVVMAELATDIGNVAKLTEGQSRATEAMVEALTTMIDEI
                     SDRFEPPTEFVVPGESAVAAALDVARTVVRRAERAAVTARRGPDSWVVPYLNRLSDLL
                     WTLARWQEGESLTTRSVPEQ"
     gene            1812..3221
                     /locus_tag="VFP02_00710"
     CDS             1812..3221
                     /locus_tag="VFP02_00710"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET9541570.1"
                     /translation="MSDAAGDADVLVSGLTADGVAEAPEGLGDHLAARGFKADLGSTV
                     LVPREGGGSRLLVGLGSEAVDAQGLRRAAAGAARALSHHRSAATTLLADAGAEDTGGA
                     QALAEGFALGAYRYGEFKSEFEPHVLEAVTVLSGVAGVADGLARGARVAEAVNVARDL
                     VNEPGGSLTPPALADRAVALGEAGGITVDIMDKAAIESAGFGGLLGVNRGSFHEPRFI
                     RMTHAPEGASGHVVLVGKGITFDAGGLSIKTADGMYGMNGDMGGGAAVIATLSAVAAL
                     APGVKVTGLVPSTDNMLGPDATRPGDVLTISNGTTVEVVNTDAEGRLILADGLAEASK
                     EAPDAIIDLATLTGACMVALGPRIAGLMSNDDTLAAKVTAAAEGAGERVWRLPLPADM
                     EKAIESPVADIKNLGNRYGGALAAGLFLQHFVADDIPWVHLDIAGPAWLDDADGENPK
                     HGTGFGVRTLLELLTTWSD"
     gene            complement(3202..>3546)
                     /locus_tag="VFP02_00715"
     CDS             complement(3202..>3546)
                     /locus_tag="VFP02_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541571.1"
                     /translation="VGHDGLDVRRRIPPLNRGQVARLPDAVQQAHLDWQEQLLVSDRF
                     EAIQRRARRLALSLFETGQDADGVERELHRWQYHPAVASAATAWALDRHRDAAAAAET
                     PDAARALSRTRS"
BASE COUNT          435 a         1259 c         1320 g          532 t
ORIGIN      
        1 ggtccgtgag gtggcgctgg cgatggcgaa gacggccacg aggccgacga caccggcgca
       61 ggccagcgcc gccgaccgcc agtcgaggtc gacgcgcagg acctcctcgg gtgctgtcgc
      121 cgctccctgg tcgccgtcgg ccacgtgggt ccggtaggcc gggtggaggg accgccggcg
      181 gacgccgtca agctgctgcg gttcacgccc ttctcggtca tccgggcggt gggcatggtg
      241 gcggggacac tgctcgtgct ggccgtcttc cgggccgcca cccgaccgct cggctggctc
      301 gtcgtcgccg tggtcggcgc cgccctgctg gggccgttcg tggccctgct ccagcgggcc
      361 cttccccgcg gactggcggt cgtcgtcgcc ctgctcgtgg ccgcggccgc cttcggtgcg
      421 gtggcgtacg tggtcgtcga cgacctgagc cgagagctga accgcgtgca gctcatctcc
      481 cgtcgggcgg ccggcgaggt cgagcgatcg gagcgctacg gcgagttcgc tcgggagatc
      541 cacctacgcg accgggtgaa cgccttcgtc gaggagctgc ccgcccggct ccgcggcggc
      601 acggacgtcg ccgccatcca gtcggccgcg tcccggggcg tctcgttcct catcaccttc
      661 gtgctgctcc tcttcatgct ggcgacaggg ccgaagttca tcgagggtgg tctgcgtcag
      721 atcgacgacg acgtcaggcg ggcgcgcgcc cgcaccttgc tgctcgagtc gtacgggcgc
      781 ttctggcggt acgtcgcggc caccctcggc cgggccgtcc tggccgggtt cttcacctac
      841 ttcgtggtcc aggtggcgga cctgcccgcg cccgtgctcc tggccgtgtg ggtcggcgcg
      901 tggagcatcg tgccggccgt cggcatcgtc gtgggctcgt tggcggtggg cctcgtggcc
      961 atcccccatt cgttctcgct ggccgggtgg ctgatggtgc tcttcatcgg ctaccaggtc
     1021 ttcgacgcgc tggtcctcga gaagcggatc gatcggtcga tcctgcacct cggctcgttc
     1081 gggacgttcg tggccgcggc gctcggactg gaggcctacg ggctcggtgg gctgatcgtc
     1141 gcgacgttgc tggccatctt cctggctgcg atggtccgga cgatcacctc gcggcccacg
     1201 ccggtggcgg tcgacgaggt gctcgccggg gctggttagc ctcgcccggg cgtgacgatc
     1261 tacaccaaga agggcgacga cgggaccacc ggcctgctgt atggcggccg ggtggccaag
     1321 gacgccgcgg cccccacggc ctacggcgag gtcgacgagg cccaggcgtt cctcggcgtc
     1381 gtgcgggccc aggccgatcg ggagctggcc ggcatcgtgc tcgggctcca gcgcgacctg
     1441 tgggtggtga tggcggagct ggccaccgac atcggcaacg tggccaagct gaccgaaggg
     1501 cagtcgcggg cgaccgaggc gatggtcgag gccctcacga cgatgatcga cgagatctcc
     1561 gaccggttcg agccgcccac cgagttcgtg gtgccgggag aatctgcggt ggccgccgcc
     1621 ctcgacgtgg cccgcaccgt ggtgcgccga gccgagcggg ccgcggtgac ggcacggcgg
     1681 ggcccggact cctgggtcgt gccctacctg aaccgtttga gtgatctgct gtggaccctg
     1741 gctcgctggc aggaaggcga gtccctgacg acgaggtcgg tgcccgaaca atgacgacga
     1801 tcgagttctc cgtgtccgat gcggccggtg atgccgacgt gctggtcagc gggctgaccg
     1861 ccgacggcgt cgccgaggcc cccgaaggac tcggcgacca cctggccgcc cgcggcttca
     1921 aggccgacct cggttcgacc gtcctggtcc cccgtgaggg tgggggctcg cgcctcctcg
     1981 tcggcctcgg ttccgaggcg gtggacgctc agggcctgcg ccgggccgcg gccggggcgg
     2041 ctcgagcgct gagccatcac cggtcggccg cgaccacgct gctggccgac gccggcgccg
     2101 aggacaccgg cggggcccag gccctggccg aaggcttcgc cctcggcgcg taccggtacg
     2161 gcgagttcaa gtccgagttc gaaccgcacg tcctcgaagc ggtgacggtc ctcagtgggg
     2221 tcgccggcgt ggccgacggt ctggcccgcg gcgcccgagt ggccgaggcc gtcaacgtgg
     2281 cccgggacct ggtcaacgag ccgggtggat cgctcacccc gcccgcgctg gccgaccggg
     2341 cggtggccct cggggaggcc ggcggcataa ccgtggacat catggacaag gcggcgatcg
     2401 agtccgccgg gttcggcggg ctgctcggcg tcaaccgggg ctcgttccac gagcctcgct
     2461 tcatccgcat gacccacgcc ccggagggag cctccggcca cgtggtgctc gtgggcaagg
     2521 gcatcacctt cgacgccggc gggctgtcga taaagaccgc ggacggcatg tacggcatga
     2581 acggggacat gggcggaggg gccgcggtga tcgccaccct gtccgcggtc gccgccctcg
     2641 ccccgggggt gaaggtgacc ggcctggtgc cgtcgaccga caacatgctc ggtcccgacg
     2701 ccacccgccc cggtgatgtc ctcacgatca gcaacgggac cacggtcgag gtggtcaaca
     2761 ccgacgccga gggtcgtctg atcctggccg atggcctggc cgaggcgtcc aaggaggcac
     2821 ccgacgccat catcgacctg gccacgctca cgggcgcctg catggtcgcc ctcggccccc
     2881 gcatcgccgg cctcatgagc aacgacgaca ccctcgccgc caaggtgacc gccgcggccg
     2941 aaggagcagg ggaacgggtc tggcgcctgc ccctgcccgc cgacatggag aaggccatcg
     3001 agtcgcccgt ggcggacatc aagaacctgg gcaaccgcta cggcggggcc ctggcggccg
     3061 ggctcttcct gcaacacttc gtcgccgacg acatcccctg ggtccacctc gacatcgccg
     3121 ggcccgcctg gctcgacgac gccgacgggg agaaccccaa gcacggcacc ggcttcggcg
     3181 tccgcaccct cctggagctc ctcacgacct ggtccgactg agcgctcggg ccgcatcggg
     3241 tgtctcggcg gcggccgcgg cgtcgcggtg ccggtcgagg gcccaggcag tggcggcgct
     3301 ggccaccgcc gggtggtact gccagcggtg gagctcgcgt tcgacaccgt cggcgtcctg
     3361 cccggtctcg aacaggctca gggccaggcg gcgggcccgg cgctggatcg cctcgaagcg
     3421 gtcgctgacc agcagttgct cctgccagtc gaggtgggcc tgctgcacgg cgtcgggcag
     3481 gcgggccacc tggccgcggt tgagcggggg gatgcggcgc cggacgtcga ggccgtcgtg
     3541 gcccac
//
LOCUS       DASTFV010000041         2527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_151032, whole genome shotgun sequence.
ACCESSION   DASTFV010000041 DASTFV010000000
VERSION     DASTFV010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151032"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1585
                     /locus_tag="VFP02_00720"
     CDS             158..1585
                     /locus_tag="VFP02_00720"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET9541572.1"
                     /translation="MRSPRPLDALLAALPEYVLVPEGIAVGVAVTAVEHDSRIAGPGS
                     LYCALKGEHTDGHRHAATAVAAGAVAVVVEHPVAVSVPQIVVPDTRAAMGRLADAFFD
                     HPSRRIAVVGVTGTNGKTTVTHLLAAVLNHVGRTTRVVGTLSGARTTPEAPALQSALA
                     EAADAGASVVAMEVSSHAIELRRIVGTWFTVAVFTNLSQDHLDFHGTMDRYFAAKTRL
                     FEPERAAVGLVNRDDPWGARLLEAIAIPAVAWSAEDAEDPELRADGSRFTWRGHRIEL
                     ALAGRFNLANAVAAAEAALALGLEPAQIAAGLAQAGPVRGRFEPVGHSDVAVLVDYAH
                     TPDGLVQALASARELTEGRLIVVFGCGGDRDRDKRPLMGRAADQGADVVIVTSDNPRQ
                     EDPAAIVETVMTGVERVDDLIVEPDRRRAVDAAVALAAPGDLVLVAGKGHETTQDLGD
                     RVIDFDDREVAAAALARRGHGEACA"
     gene            1582..>2527
                     /gene="mraY"
                     /locus_tag="VFP02_00725"
     CDS             1582..>2527
                     /gene="mraY"
                     /locus_tag="VFP02_00725"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET9541573.1"
                     /translation="MRTLLFAAGVSLMVSLLGTKVLIDVLTRRRIGQPIREDGPQGHT
                     TKAGTPTMGGAAIVGGAVVGYLASDISNSRIITRSGLVVMLALVGAGLVGLADDWIKV
                     TRERNLGLTSRTKFLGLLAVALTFAVLAVGHTPVSTDLSFTRSGVLLDLGEIGWCVWA
                     VLLIVGTTNAVNLTDGLDGLAGGAATLAFTAFIVIGFWAFRHPLVYGVPHALDLAVVA
                     ACMTGACVGFLWWNASPAQIFMGDTGSLAIGAALAGLALMTNTSLLLALVGGLFVVET
                     LSVIIQVASFQIFGRRVFRMAPIHHHFELRGWPETTVII"
BASE COUNT          312 a          959 c          873 g          383 t
ORIGIN      
        1 gacccgcagc tctcgatcgt ggtcgtgatc gacgagccct ccggcgacgt ctacggcggc
       61 gacgtgtccg gaccggtgtt cagccggctg gccggagccg tcctccgggc ctaccggatc
      121 ccaccgccct cctccatcgt cgaggcaggc tgagcccatg cgctccccgc gaccgctcga
      181 cgcgctcctg gccgcgctgc ccgagtacgt cctcgtgccg gagggcatcg ccgtcggcgt
      241 cgcggtcacc gccgtcgagc acgacagccg catcgccggt cccggctcgc tgtactgcgc
      301 gctcaagggg gagcacaccg acggccatcg tcacgcggcc actgcggtgg cggcaggcgc
      361 ggtcgcggtg gtcgtcgagc accccgttgc ggtcagtgtc ccgcagatcg tggttcccga
      421 cacccgcgcc gcgatgggac gcctggccga cgccttcttc gaccacccgt ctcgacggat
      481 cgccgtcgtc ggggtgacgg gcaccaacgg caagaccacg gtgacccacc tgctggccgc
      541 cgtgctcaac cacgtcggtc ggaccacccg tgtcgtgggc accctgagcg gggcccggac
      601 caccccggag gctccggcgt tgcagtccgc cctggccgag gcggccgacg ccggcgcctc
      661 ggtcgtggcc atggaggtct cctcgcacgc catcgagctg cgccgcatcg tcggcacctg
      721 gttcacggtg gccgtcttca ccaacctctc ccaggaccac ctggacttcc acgggacgat
      781 ggaccgctac ttcgcggcca agacccgcct cttcgagccg gaacgggctg ccgtcggcct
      841 ggtgaaccgc gacgatccct ggggcgcccg cctgctcgag gccatcgcca tccccgccgt
      901 ggcctggtcg gccgaggacg ccgaggaccc cgagctccgg gccgacggca gccgcttcac
      961 gtggcggggc caccgcatcg agctggccct cgccggccgc ttcaacctgg ccaacgccgt
     1021 cgccgccgcc gaggccgccc tggccctcgg cctcgagccg gcccagatcg ccgccggcct
     1081 ggcccaggcc gggcccgtcc gcggccgctt cgagccggtc ggacacagcg acgtggccgt
     1141 gctcgtcgac tatgcccaca cgcccgacgg cctcgtccag gccctggcgt cggcccgaga
     1201 gctcacggag ggccggttga tcgtggtgtt cggctgcggc ggcgaccggg accgggacaa
     1261 gcgcccgctg atgggccgcg ccgccgacca gggggccgac gtcgtgatcg tgacctcgga
     1321 caatccccgt caggaggacc cggcggccat cgtcgagacc gtgatgaccg gcgtggaacg
     1381 ggtcgacgac ctgatcgtgg agcccgaccg ccgtcgggcc gtcgacgcgg ccgtggccct
     1441 cgccgcgccg ggggacctgg tcctggtggc gggcaaaggg cacgagacga cccaggacct
     1501 gggtgaccgg gtgatcgact tcgacgaccg ggaggtggcc gccgccgccc tcgcccgccg
     1561 tggccatggg gaggcgtgcg cgtgaggacg ctcctgttcg cggccggcgt ctccctgatg
     1621 gtgtcgctgc tcggcaccaa ggtcctgatc gacgtgctga cccggcgccg gatcggccag
     1681 cccatccgag aggacgggcc gcagggccac accaccaagg ccggcacgcc gacgatgggc
     1741 ggggccgcca tcgtcggtgg ggccgtggtc gggtacctgg cctccgacat ctccaacagc
     1801 cgaataatca cccgatcggg cctggtcgtg atgttggcct tggtcggcgc ggggctggtc
     1861 ggcctcgccg acgactggat caaggtcacc cgggagcgga acctcggact gacgtcacgg
     1921 accaagttcc tgggcctcct ggccgtggcc ctcaccttcg ccgtgctggc cgtgggccac
     1981 acgccggtca gcaccgacct ctccttcacc cggtcgggcg tcctgttgga tctcggggag
     2041 atcggctggt gcgtgtgggc cgtgctcctg atcgtgggca cgaccaacgc cgtcaacctg
     2101 accgacgggc tcgacggcct ggccggtggg gccgccaccc tcgccttcac cgccttcatc
     2161 gtgatcgggt tctgggcctt ccgtcacccg ctggtctacg gcgttcccca cgccctggac
     2221 ctggccgtcg tcgccgcgtg catgacgggg gcgtgtgtcg gcttcctctg gtggaacgcc
     2281 tcgcccgccc agatcttcat gggcgacacc ggctcgttgg ccatcggcgc cgccctggcc
     2341 ggcctggccc tgatgaccaa cacgtcgctg ctcctggcgc tcgtcggcgg cctgttcgtg
     2401 gtcgagaccc tgtcggtcat catccaggtg gccagcttcc agatcttcgg gcgccgggtc
     2461 ttccgcatgg cgccgatcca tcaccacttc gagctccggg gctggcccga gacgacggtg
     2521 atcatcc
//
LOCUS       DASTFV010000042         5410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_153261, whole genome shotgun sequence.
ACCESSION   DASTFV010000042 DASTFV010000000
VERSION     DASTFV010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153261"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VFP02_00730"
     CDS             complement(<1..799)
                     /locus_tag="VFP02_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541574.1"
                     /translation="MTTLDDLRIRVVPGDGVVLRAPGVTAVAFPSNEEHQTLVTAFLD
                     AARAAAGQPDAGRKAARAVAGLLAAAEDAPPLGLVGTDGAGLAVLLHGDVEFAFRDAA
                     GVDQTLSGRDAAAWLDRLLDTVPEQWSLARDGAGAADEWSDLQAGVARGNGVATGTPA
                     PEAETAADVPVPPVEPAPEQAPAPQVDVAPPPPPPEVATAPVPAADFVSVSLLDEEPE
                     GGSVRAPLPLAGADEPPPPPEPNEAEVPHVHGVICARGHFNDPTSPFC"
     gene            complement(804..1280)
                     /locus_tag="VFP02_00735"
     CDS             complement(804..1280)
                     /locus_tag="VFP02_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541575.1"
                     /translation="MLPTLNGRIQTRIFLVGVVGSVWTLIITPVVALFVDGDPTLGDV
                     YHVTFEILALVLILGIGWEFVYELLMQFRWEKDWPTMFGYLTGINEGILVYVVADKLG
                     SNPDTEWRAYLGAITIVPFLIHFVSTWLVINLFAGNFMKMIFIRWRFKGGRLIGGW"
     gene            complement(1296..2876)
                     /locus_tag="VFP02_00740"
     CDS             complement(1296..2876)
                     /locus_tag="VFP02_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541576.1"
                     /translation="MSKTDSVSLPYEEIAAAMEAAQAPGDLTDQLIATSEWSDQMLAE
                     AGIPVVDVPIVSVGGGLGSFVLADYLRQAGMPATSFKALGALDNPWDTYEYLTRCSQI
                     PRPERLRSDSGSTPDNIWGFPSYATREAMAAPGLVKKLDPLFNVATEPILTDYFTPRA
                     GQVFEALEVEARRIGYDQMKAKGIVRMVRKRHGGGYFTILTPEAGTSDTKRVAYRSTY
                     VHVAVGYPGLRFLPDLQEYRNTHEDFHRVVNAYEPHEHVYEELAKKPGTVMVRGAGIV
                     GSRILQRLCDDRDKGSQIQIVHLFRTYVDSSHGPNIFLRRKGSHGWAFQGFNYPKSCW
                     GGQYKHNLERWPDDKRNALWDTLGGTTTPHRKLWLNQLKKGRAEGWYREMVGTVDSVT
                     PGDGTVISRVNTNQGIFDVPANYVIDGTGLEADITEHRVLADLLQHGGAVRNPKGKLQ
                     VETYFEVRGTRSGVGKLYASGSATLGSYFAVVDSFLGLQYAALRIADDLADEGFVPRI
                     GVRRSMSQWRRWRRKTAP"
     gene            complement(2956..5013)
                     /locus_tag="VFP02_00745"
     CDS             complement(2956..5013)
                     /locus_tag="VFP02_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9541577.1"
                     /translation="MPETWLFAAVPGALTEEITARVFLSIAIIIVVARVFGRLAQKVR
                     QPAVVGEIVAGIMLGPSFLGLINEDLPTKLFPLAVRPFLGIIAQVGLIIFMFIVGLEL
                     DMGLIRGKGRLAAVTSLTSVVLPFTFGLAISGVLHEAYGVVNGQEVEWLHFALFMGAA
                     MSVTAFPVLARILNERGMHRTRIGVLTLACAAVDDVLAWSLLAGVLAVVGEGHLAPYW
                     VIILTLVYGAVMVVLIRPLLQKVVDRYQEVGRLTPDLLAVVLVGVMVSSYITSEIGIH
                     AIFGAFFFGAVFPREGAHQMFHEILERLEQVTVLLLLPVFFVVTGLNVDITGLGVDGL
                     GYLLLVILAACAGKFLGASLASRSQGVPARQALALGVLMNTRGLTELVILSIGREVGV
                     LNDAMFTIMVVMAVFTTIITEPLLRILYPDELVAREIAEAERIGSAEAEAFRVLVGTP
                     EGHPGPTLATLAAELTEGEASREVALVQFGTPDDTLEVGGGIEAMATSLDALHHLETA
                     VEASGVPVFARSRIDEDPGRLLGLLADESVADTILVPEDPSYTPPGGSQSPAAPTARL
                     SLNGATSTNGEVVVVLHGGGDDIHALDLGLRLAASRGAPLHVTGSPGRRVGSSLARLR
                     SEGLVDETAPAVAGLEIHAGEVSSGPAAGPTLGVWSGRDDGAERLVALVDRLGRPAPA
                     TEV"
     gene            5116..>5410
                     /locus_tag="VFP02_00750"
     CDS             5116..>5410
                     /locus_tag="VFP02_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9541578.1"
                     /translation="METIADYRLTRSLGAGSHGEFFLAEPPARLGLDAEFVAVKVMAG
                     QTQEDAFRRATRELRLFASVKSPFLVALYDAGQEGGRFFYAAEYLPLGSLASPA"
BASE COUNT          834 a         1765 c         2046 g          765 t
ORIGIN      
        1 cacagaacgg tgaggtgggg tcgttgaagt gcccccgggc gcagatcacg ccgtggacat
       61 ggggcacctc ggcctcgttg ggttcgggtg ggggcggcgg ctcgtcggcc ccggcgagcg
      121 ggagcggggc ccgcaccgac ccgccctcgg gctcctcgtc gagcagtgac accgacacga
      181 agtccgcggc cggcaccgga gcggtggcca cctcgggcgg tggcggggga ggggccacgt
      241 cgacctgggg ggcaggcgcc tgctccggcg cgggctcgac cggcggcacc ggcacgtccg
      301 ccgccgtctc cgcctccggc gcgggcgtgc cggtggccac tccgttgccc cgggccacgc
      361 cggcttggag gtcggaccac tcgtcggcgg cacccgcccc gtcgcgggcc agtgaccact
      421 gctcgggcac ggtgtcgagg aggcggtcca gccaggccgc ggcgtcccgc ccggagaggg
      481 tctggtcgac cccggcggcg tcccggaagg cgaactcgac gtcgccgtgc aggaggacgg
      541 cgaggccggc gccgtcggtg cccaccagac ccagcggcgg tgcgtcctcg gctgcggcga
      601 gcaggccggc cacggcccgt gccgccttgc gcccggcgtc gggctggccg gcggcggcgc
      661 gcgccgcgtc gaggaaggcc gtcaccagag tctggtgctc ttcattgctg gggaaggcga
      721 cagcggtgac gcccggggcc cggagcacga cgccgtcgcc ggggaccaca cggattcgga
      781 ggtcgtcgag ggtggtcatc ggatcaccag cctccgatca ggcgtccgcc cttgaagcgc
      841 cagcggatga agatcatctt catgaagttc ccggcgaaga ggttgatgac gagccaggtg
      901 ctcacgaagt ggatgaggaa ggggacgatg gtgatggcgc ccaggtaggc ccgccactcc
      961 gtgtccgggt tcgagccaag cttgtcggcg acgacgtaca cgaggatccc ctcgttgatc
     1021 ccggtgaggt agccgaacat cgtcggccag tccttctccc accggaactg catgaggagc
     1081 tcgtaaacga actcccaacc gatgccgagg atcaggacga gggccaggat ctcgaaggtg
     1141 acgtggtaga cgtcgcccag cgtcgggtcg ccgtcgacga acagggcgac gacgggggtg
     1201 atgatgagcg tccagaccga gcccacgacc cccacgagga agatgcgggt ctggattcgg
     1261 ccgttgagcg tgggtagcac ggggctcctc gatcctcagg gggcggtctt gcggcgccag
     1321 cggcgccact gcgacatgga ccggcgcacg cctatgcggg ggacgaagcc ctcgtcggcc
     1381 aggtcgtcgg cgatacgcag ggcggcgtac tggaggccga ggaacgagtc gacgaccgcg
     1441 aagtaggagc cgagggtcgc cgaaccagag gcgtacagct tgccgacccc gctccgggtg
     1501 ccgcggacct cgaagtaggt ctcgacctgg agcttcccct tggggttgcg caccgcacca
     1561 ccgtgctgga ggaggtcggc caggacccgg tgttcggtga tgtcggcctc gaggccggtg
     1621 ccgtcgatga cgtagttggc ggggacgtcg aagatcccct ggttggtgtt gacccggctg
     1681 atgacggtcc cgtcgcccgg ggtgaccgag tcgacggtgc cgaccatctc ccggtaccag
     1741 ccctcggccc ggcccttctt gagctggttc agccagagct tgcggtgggg ggtggtcgtg
     1801 cccccgagcg tgtcccacag cgcattgcgc ttgtcgtcgg gccagcgctc gaggttgtgc
     1861 ttgtactggc cgccccagca ggacttcggg tagttgaagc cctggaaggc ccacccgtgg
     1921 ctgcccttgc ggcgcaggaa gatgttcggg ccgtgggagc tgtcgacgta ggtccggaag
     1981 aggtggacga tctggatctg gctccccttg tcccggtcgt cacacagccg ctgcaggatc
     2041 cgggagccga cgatgccggc cccccgcacc atgaccgtgc ccggcttctt ggccagctcc
     2101 tcgtagacgt gctcatgggg ctcgtaggcg ttgacgaccc ggtggaagtc ctcgtgggtg
     2161 ttgcggtact cctgcaggtc gggcaggaag cgaagaccgg ggtagcccac ggccacgtgc
     2221 acgtacgtgc tgcggtacgc cactcgcttg gtgtcgctgg tgccggcctc gggggtgagg
     2281 atcgtgaagt agcccccgcc gtggcgcttg cgcaccatgc gcacgatgcc cttggccttc
     2341 atctggtcgt acccgatgcg acgggcctcg acctcgaggg cctcgaagac ctgccccgcc
     2401 cgtggcgtga agtagtcggt gaggatgggc tcggtggcga cgttgaagag ggggtccagc
     2461 ttcttgacga ggccgggggc ggccatggcc tcccgggtgg cgtaggaggg gaacccccag
     2521 atgttgtccg gcgtcgagcc ggaatcggac cggagccgct cggggcgggg gatctgggag
     2581 cagcgggtca ggtactcgta ggtgtcccac ggattgtcca acgccccgag ggccttgaag
     2641 ctggtggcgg gcatgcccgc ctggcgcagg tagtcggcga gcacgaacga gccgagaccg
     2701 ccgccgacgg agacgatggg gacgtcgacg acggggatgc cggcctcggc cagcatctgg
     2761 tcgctccact cgctcgtggc gatgagctga tcggtcaggt cgcccggcgc ctgcgccgct
     2821 tccatggccg cggcgatctc ctcgtacggg aggctgactg agtcggtctt gctcatcctg
     2881 ggctcctcgt cagtccgccg gggccagcgg gtggcgggat gatagggcac gccggcaggc
     2941 ccgtcggcgt gcgggtcaga cctccgtggc gggggcgggt cggccgaggc ggtcgacgag
     3001 ggcgacgagg cgctccgcgc cgtcgtcgcg accgctccag acgccgaggg tgggtcccgc
     3061 ggcgggcccg gacgacacct cgccggcgtg gatttccagc ccggcgaccg cgggagcggt
     3121 ctcgtccacg aggccctccg accggagccg ggcgaggctt gacccgaccc ggcgcccggg
     3181 ggacccggtg acgtgcaggg gcgcaccccg ggatgcggcg agccgcagcc cgaggtccag
     3241 ggcgtggatg tcgtcaccgc cgccgtggag cacgaccacc acctcaccgt tggtcgacgt
     3301 ggcgccgttg agcgacaagc gggcggtggg agcggccggg ctctgactgc cgcccggcgg
     3361 ggtgtaggag ggatcctccg ggaccaggat cgtgtcggcc acgctctcgt cggccagcag
     3421 gccgagcagg cgacccgggt cctcgtcgat gcgggaccgg gcgaacacgg ggacgccgga
     3481 cgcctcgacc gcggtctcca ggtggtggag ggcgtccagg gaggtggcca tggcctcgat
     3541 gccaccgccg acttcgagcg tgtcgtcggg cgtgccgaac tggaccaggg ccacctcgcg
     3601 ggaggcctcg ccctcggtga gctcggcggc cagcgtggcc aacgtcggcc ccgggtgccc
     3661 ttcgggcgtg ccgaccagca cgcggaacgc ctcggcctcg gccgagccga tgcgctccgc
     3721 ctcagcgatc tcccgggcga ccagctcgtc cgggtagagg atgcgcagga ggggctcggt
     3781 gatgatcgtc gtgaagacgg ccatcaccac catgatcgtg aacatggcgt cgttgaggac
     3841 cccgacctcg cgcccgatgg agaggatgac cagctcggtg aggccccggg tgttcatgag
     3901 gacgccgagc gccaaagcct ggcgggcagg gacgccctgg ctgcgagaag ccagcgaggc
     3961 gccgaggaac ttccccgcgc aggcggccag gatgacgagc aggaggtagc cgaggccgtc
     4021 cacgccgagg ccggtgatgt cgacgttgag gcccgtcacc acgaagaaga ccggtaggag
     4081 gagcaggacc gtcacctgtt cgagccgttc caggatctcg tggaacatct ggtgggcacc
     4141 ttcacggggg aacaccgccc cgaagaagaa ggccccgaag atggcgtgga tgccgatctc
     4201 agaggtgatg taggacgaga ccatcacgcc gaccaggacg acggcgagga gatcgggggt
     4261 gagccggccg acctcctggt accggtcgac gaccttctgg agcagcggtc ggatcaggac
     4321 gaccatcacg gcgccgtaga cgagggtcag gatgatcacc cagtacggcg ccaggtgccc
     4381 ttcgccgacc acggcgagga ccccggccag gagggaccag gccaggacgt cgtccacggc
     4441 cgcgcaggcc agggtcaata cgccgatccg ggtccggtgc atgccccgct cgttgaggat
     4501 gcgggccagg accgggaacg cggtgacgga catggcggcg cccatgaaga gggcgaagtg
     4561 cagccactcg acctcctggc cgttgaccac tccgtaggcc tcgtggagga cgccggagat
     4621 ggccaggccg aaggtaaagg ggagcacgac cgaggtcagc gaggtcacgg cggccagtcg
     4681 gcccttgccg cggatgaggc ccatgtccaa ctcgagcccc acgatgaaca tgaagatgat
     4741 gaggccgacc tgggcgatga tgccgaggaa aggccggacc gcgagcggga acagcttggt
     4801 cggcaggtcc tcgttgatga gcccgaggaa gctggggccg agcatgatcc cggccacgat
     4861 ctcgcccacg accgcgggtt gacggacctt ctgggccaga cgcccgaaga cccgggccac
     4921 cacgatgatg atggcgatgg agaggaagac ccgagcggtg atttcctcgg tgagtgctcc
     4981 aggaacggcg gcgaacaacc aggtctccgg cacggctcct ccgagcgtca gggcggccgg
     5041 atcgtagcaa cccggcgaag gcctagagtg cggggccgtc cgtggccgac aggacccggg
     5101 gcacaggagg cctgtgtgga gacgatcgcc gactaccgcc tgacccggtc actcggggcg
     5161 ggtagccacg gggagttctt cttggccgaa cctcccgccc ggctcggact cgacgccgag
     5221 ttcgtcgccg tcaaggtgat ggccggccag acccaggagg acgccttccg gcgggccacc
     5281 cgggagctcc gcctcttcgc ctcggtgaaa tcgccgttcc tggttgcgct ctacgacgcc
     5341 ggccaggagg gtgggcgctt cttctacgcc gccgagtacc ttcccctcgg ctcgttggcg
     5401 tcgccggcca
//
LOCUS       DASTFV010000043         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_153746, whole genome shotgun sequence.
ACCESSION   DASTFV010000043 DASTFV010000000
VERSION     DASTFV010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153746"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VFP02_00755"
     CDS             <1..792
                     /locus_tag="VFP02_00755"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET9541579.1"
                     /translation="GDTDTAPFGMGTYASRSTPVAGAAVAMVSRKIKAKARKLAAHLL
                     EVSEEDVEWELGRFYVRSAPDQGVTIQECAMAAYSNMPDGMEPGLENNAYYDPPNLTW
                     PFACYIATVEVDPGTGVWDVLDVVAVDDCGVRINPMIVEGQIMGGLTEAYAMASMQFI
                     TFDAEGNCIGSNYMDYLLPTAWETPGFQLHEVVTPCPHHPIGAKGVGECATVGGPAAF
                     VNAVMDALKGTGVRNIDMPLLPDRVYAALQDGQDVSGAPPVKTGS"
     gene            803..1387
                     /locus_tag="VFP02_00760"
     CDS             803..1387
                     /locus_tag="VFP02_00760"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9541580.1"
                     /translation="MLIATDFEVGQPVDKVWAFFDDIPQVAACLPGAELTDEVGPDAY
                     KGKVAIRMGPVKLQFAGTATIKERDEEAKRLVVDAAGADEKGRGQAAMLLTAVLSPAG
                     SGTKVEVSQDLQLSGAAAQYGRGMVADVTAVLMKDFSRNLQDRMTALEQGRSADEITS
                     AAPAGGFAIGLAALRMALARVFRRFFLPYRPSPS"
     gene            1399..1665
                     /locus_tag="VFP02_00765"
     CDS             1399..1665
                     /locus_tag="VFP02_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541581.1"
                     /translation="MALWWIGNALLLLVVVPVLVALLNRVLAALERIRGASDDILTGG
                     VALVGELDAVPGLLATTGGTIHDVSVGAVRYAGSVAQLLPPKED"
     gene            1665..1892
                     /locus_tag="VFP02_00770"
     CDS             1665..1892
                     /locus_tag="VFP02_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541582.1"
                     /translation="MPAAAIVTLVLATLIIAAAALGLIRVILHLSAVARTLDGVVGGV
                     QAVADKTAPVPAAVAAVNADLAPVRDFCESI"
     gene            1905..2177
                     /locus_tag="VFP02_00775"
     CDS             1905..2177
                     /locus_tag="VFP02_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541583.1"
                     /translation="MLTVLAATSDSTVSWDIGYAIAVIVVLAVVALVVPILFLAYRIG
                     TQAGAINDALTDAVDNTSALAGLKTTNESATAIVAGLERGRTRLGG"
     gene            2174..2464
                     /locus_tag="VFP02_00780"
     CDS             2174..2464
                     /locus_tag="VFP02_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541584.1"
                     /translation="MTVLALTETQEDLWWGAVIGGFVVVLAVAALLTILVLLVRRIDQ
                     RVVAVRDSLQAAAANTADTALLAETAAGVEAVLSEGLKHHLFLGRVRQAVRS"
BASE COUNT          377 a          960 c          890 g          388 t
ORIGIN      
        1 ggcgacaccg acaccgcgcc gttcgggatg ggcacctacg cctcgcgcag cactccggtg
       61 gccggggccg cggtcgcgat ggtgtcccgc aagatcaagg ccaaggcccg caagctggcc
      121 gcgcacctgc tggaggtgtc cgaggaggac gtcgagtggg agctgggccg cttctacgtg
      181 cgcagcgcac cggaccaggg cgtcaccatc caggagtgcg cgatggcggc gtacagcaac
      241 atgcccgacg gcatggagcc agggctcgag aacaacgcct actacgaccc gcccaacctc
      301 acctggccct tcgcctgcta catcgccacc gtggaggtgg acccggggac cggggtctgg
      361 gacgtgctcg acgtcgtggc ggtggacgac tgcggggtgc gcatcaaccc catgatcgtc
      421 gagggccaga tcatgggggg cctgaccgag gcctacgcca tggccagcat gcagttcatc
      481 accttcgacg ccgagggcaa ctgcatcggg tccaactaca tggactacct gctgccgacg
      541 gcgtgggaaa cgccggggtt ccagctccac gaggtggtca ccccctgccc ccaccacccg
      601 atcggggcca agggcgtcgg cgagtgcgcc accgtcggcg ggccggccgc cttcgtgaac
      661 gcggtgatgg acgccctgaa gggcaccggc gtgcgcaaca tcgacatgcc cctcctgccc
      721 gaccgggttt atgccgcgct tcaggacggc caggacgtct ccggcgcccc gccggtgaag
      781 acgggatcgt gagggcgccc cgatgctgat agccaccgac ttcgaggtcg gccagccggt
      841 cgacaaggtc tgggccttct tcgacgacat cccccaggtg gccgcctgcc ttcccggcgc
      901 cgagctgacc gacgaggtcg ggcccgacgc ctacaagggc aaggtcgcca tccgcatggg
      961 gccggtcaag ctccagttcg cgggcacggc cacgatcaag gagcgcgacg aggaggccaa
     1021 gcgcctcgtc gtcgacgccg ccggcgcgga tgagaagggc cggggccagg ccgccatgct
     1081 gctgaccgcc gtgctctccc cggcgggcag cggcaccaag gtcgaggtga gccaggacct
     1141 ccagctgtcc ggtgccgcgg cccagtacgg gcggggcatg gtcgccgacg tcaccgccgt
     1201 gctgatgaag gacttctccc ggaacctgca ggaccggatg acggcgctcg agcagggccg
     1261 ctcggccgac gagatcacgt ccgccgcccc cgccggtggc tttgccatcg ggctggccgc
     1321 cctgcgtatg gccctggccc gggtcttccg gcgcttcttc ctgccctacc gaccgagccc
     1381 gagctgagga gacgacccat ggcgctctgg tggatcggca atgccctgct gctgttggtg
     1441 gtggtccccg tactggtggc cctgctcaac cgcgtgctcg ccgccctcga acgcatccgg
     1501 ggggcgtccg acgacatcct gaccggcggc gtggctctcg tcggtgagct ggacgccgtc
     1561 cccggcctgc tggccacgac cggcggcacc atccacgacg tctcggtcgg cgccgtgcgt
     1621 tacgccggct ctgtcgccca actgctcccg ccgaaggagg actgatgccc gccgccgcca
     1681 tcgtcaccct ggtcctcgcc accctcatca tcgccgccgc cgccctcggc ctgatccggg
     1741 tgatcctgca cctcagcgcc gtggcccgca ccctcgacgg cgtggtcggc ggcgtccagg
     1801 ccgtcgccga caagaccgca cccgttcccg cggccgtcgc cgcggtgaac gccgacctcg
     1861 cgccggtgcg cgacttctgc gaatcgatct gaggaggcga caccatgctc accgtcctcg
     1921 ccgccaccag cgactccacc gtcagctggg acatcgggta cgccatcgcc gtgatcgtcg
     1981 tcctggcggt cgtcgccctg gtcgtgccga tcctgttcct ggcctatcga atcgggaccc
     2041 aggccggcgc catcaacgac gcgctgaccg acgccgtcga caacacctcg gctctggccg
     2101 ggctcaagac cacgaacgag tcggcgacgg ccatcgtggc cgggctcgag cggggccgga
     2161 cccggttggg aggatgaccg tgctggcact gaccgaaacg caggaggacc tgtggtgggg
     2221 ggcggtcatc ggggggttcg tggtcgtcct ggccgtggcc gccctgctga ccatcctggt
     2281 gctgctggtg cgccgcatcg accaacgggt cgtggccgtg cgtgactcgc tgcaggcggc
     2341 ggcggccaac accgcggaca cggcgcttct cgccgagacc gccgccgggg tcgaggccgt
     2401 cctctccgag ggcctcaagc accatctctt cctgggccgg gtccgccagg cggtgcgctc
     2461 gtgaccaccc tcgtcgtcct ctccgtcgtc gacatcgtcc tgctcatcgc cgggctggcc
     2521 atctacctgt ggatcgtcgg cggccagctc cagaacgtcg ccaccaacct cgaggagtgc
     2581 tccgagatcg tgcagacgat cctgagcgac gccga
//
LOCUS       DASTFV010000044         3015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_154912, whole genome shotgun sequence.
ACCESSION   DASTFV010000044 DASTFV010000000
VERSION     DASTFV010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154912"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VFP02_00785"
     CDS             <1..659
                     /locus_tag="VFP02_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541585.1"
                     /translation="ADVEVSEGGIRVRRSRPEPFAYTVPGDPSQAAFWVVAATLVPGS
                     DLVVEDLYLGPARTGYLDVLARMGADIEVERDADAGTGTVRVRAAALHGTSVVGAEIV
                     GLDEVPALAVAAAAAEGTTRFVDVGELRVKESDRLATVSGSVRAMGGTAEVDGDALVI
                     EGGTLQGGHIDAHGDHRIAMAAAVAGLASAGEAVTVEGWDAVATSYPRFEQDLGECTP
                     "
     gene            656..1303
                     /gene="cmk"
                     /locus_tag="VFP02_00790"
     CDS             656..1303
                     /gene="cmk"
                     /locus_tag="VFP02_00790"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET9541586.1"
                     /translation="MTVIAIDGPGGSGKSTVARHLAERLDLEYLDTGAMYRAVAFAAL
                     RRGIDPSDTEPVAKLVDGLELTVGEDGVMVDGVDATIEIRGPEVTRAVSLVAANPAVR
                     AELRSRQREWAAARPGGVVEGRDIGTVVFPDATLKVFLTARADVRAERRSQEVTDLDY
                     ETVAADLARRDTLDEGREDSPLAQAEDALVLDTSGLSIDEVVASLVVALAEATAS"
     gene            1300..2004
                     /locus_tag="VFP02_00795"
     CDS             1300..2004
                     /locus_tag="VFP02_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9541587.1"
                     /translation="MTKYESRYGSADPTLLSRWFYRFVWSLIWGAGKIMFRIEVHGRD
                     NIPATGAFILAPGAHRSNIETFVVSLVTRRRMRFMGKDSLWKYAGPDWFFSSLGGFPI
                     NRDGADREALTQCLELVAAGEPVVMFPEGTRRSGPTIEVDHMRDGVAYVANRGQTPIV
                     PVGIGGSERVMPAKAKYLRPVKMVLVVGEPLAPPPLKESGRVSRSAVRAQTEELRLRL
                     QALFDDAQRRAGTPNT"
     gene            2014..2412
                     /locus_tag="VFP02_00800"
     CDS             2014..2412
                     /locus_tag="VFP02_00800"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HET9541588.1"
                     /translation="MALAGEPGHEHPRWDRLRDAAIEAEFDTGRREDSVEEARAAIHI
                     RLARMTVGSLLLVAGVCMLVLPGPGWLAIAGGLAILSKDVVWAERALERVRRRLPADE
                     DGSVSTPVIVLSVLVALGAVSLSVWWYLLR"
     gene            2409..>3015
                     /locus_tag="VFP02_00805"
     CDS             2409..>3015
                     /locus_tag="VFP02_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9541589.1"
                     /translation="MSDPEAAIRLEGVSKTFPGSTRPAVGSLDLTVPAGRITVLVGPS
                     GCGKTTTLKMINRLIEPTGGRLEVLGAEIREIPVHELRRQIGYVIQQIGLFPHKTIAE
                     NIATVPKLLGWEKARTRQRVDELTELVGLDPEHLRRYPAALSGGQQQRVGVVRALAAD
                     PPVLLMDEPFGAVDPIVRARLQRELLDLQEKVRKTIVLVTHD"
BASE COUNT          414 a         1085 c         1086 g          430 t
ORIGIN      
        1 gggccgacgt cgaggtgagt gagggcggca tccgggtgcg ccgctcccgc cccgagccgt
       61 tcgcctacac cgtccccggc gacccgtcac aggccgcttt ctgggtggtg gcggccacgc
      121 tcgtcccggg tagcgacctg gtcgtcgagg acctctacct cggccccgcc cggactggct
      181 acttggacgt cttggcgcgc atgggcgctg acatcgaggt cgaacgcgac gccgacgccg
      241 ggacgggcac ggtccgggtc cgtgccgcgg ccctgcacgg gacctccgtg gtcggcgccg
      301 agatcgtcgg cctggacgag gtcccggcgc tggccgtggc cgccgccgcg gccgagggga
      361 cgacgcgctt cgtcgacgtg ggtgagctcc gggtcaagga gtcggaccgc ctggccacgg
      421 tgtcggggag cgtccgggcc atgggcggca ccgcggaggt cgacggcgac gcgctggtca
      481 tcgagggcgg caccctccag ggtggccaca tcgacgccca cggtgaccac cgcatcgcca
      541 tggccgccgc cgtggccggg ctggcttcgg cgggcgaagc ggtgaccgtc gaggggtggg
      601 acgccgtggc caccagctat ccccgtttcg aacaggatct gggagagtgc acgccatgac
      661 ggtgattgcc atcgacggtc cgggcggctc cggcaagtcg accgtggccc gccacctggc
      721 cgagcgcttg gacctcgagt acttggacac cggagccatg taccgagcgg tagctttcgc
      781 cgccctgcgc cgggggatcg acccgtccga caccgagccg gtggcgaagc tggtcgacgg
      841 cctcgagctg acggtggggg aggacggggt gatggtcgac ggggtcgacg ccaccatcga
      901 gatacgaggc ccggaggtca cgcgggcggt cagcctggtg gcagccaacc ccgccgtacg
      961 ggccgagctc cgcagccggc aacgggagtg ggccgcggcc cggcccggtg gcgtcgtgga
     1021 gggtcgcgac atcggcaccg tggtcttccc ggacgccacc ctcaaggtct tcctgacggc
     1081 ccgggccgac gtgcgggccg aacgccgctc ccaggaggtg accgacctcg actacgagac
     1141 ggtggccgcc gacctggccc ggcgagacac cctggacgag gggcgggagg acagccctct
     1201 ggcccaggcc gaggacgcct tggtcctcga caccagcggc ctctccatcg acgaggtggt
     1261 ggcgtccctg gtcgtcgctc tcgctgaggc gaccgcctcg tgaccaagta cgagagccgg
     1321 tacggcagcg ccgatccgac gttgctgtcg cgctggttct accggttcgt gtggagcctc
     1381 atctggggcg cggggaagat catgttccgc atcgaggtcc acgggcggga caacatcccc
     1441 gccacgggcg ccttcatcct cgcccccggt gcccaccgct cgaacatcga gacgttcgtg
     1501 gtctcgttgg tgacccgccg tcggatgcgg ttcatgggca aggactccct ctggaagtac
     1561 gccggccccg actggttctt ctcgtcgctg ggcgggttcc cgatcaaccg cgacggcgcc
     1621 gaccgagagg cgctcaccca gtgcctcgag ctggtcgcgg ctggagagcc cgtggtgatg
     1681 ttcccggagg gcacgcggcg ttcggggccg accatcgagg tcgatcacat gcgcgacggc
     1741 gtggcctacg tcgccaatcg gggccagacc ccgatcgtgc ccgtcggcat cggcggctcg
     1801 gagcgggtca tgccggcgaa ggccaagtac ctccggcccg tgaagatggt cctcgttgtc
     1861 ggcgagccgc tggccccgcc cccgctcaag gagagcgggc gggtgtcccg cagcgccgtc
     1921 cgggcccaga ccgaagagct gcgcctgcgc ctgcaggcgc tgttcgacga cgcccaacga
     1981 cgcgccggta caccgaacac ctagcctgcc cagatggcgc tggcgggcga gcctgggcac
     2041 gagcacccgc gatgggaccg cctccgggac gccgccatcg aggccgagtt cgataccggc
     2101 cgccgcgagg actccgtcga agaggcccgg gcggcgatcc acatccgcct cgctcgcatg
     2161 accgtcggct ccctcctgct cgtcgccggc gtgtgcatgc tcgtcctgcc gggccccggc
     2221 tggctggcca tcgccggggg cctggccatc ctgtccaagg acgtggtctg ggccgagcgg
     2281 gccctcgaac gggtccggcg ccgcctgccc gccgacgagg acggcagcgt ctccacgccg
     2341 gtcatcgtcc tgtcggtcct cgtcgccctc ggcgccgtga gcctgtccgt ctggtggtac
     2401 ctcctccggt gagtgacccc gaggcggcca tccggctgga gggtgtctcc aagaccttcc
     2461 ctggctcgac ccgaccggcg gtcgggtcgc tcgacctcac cgtccccgcc ggccggatca
     2521 ccgtgctggt cgggccatcg ggctgcggca agacgaccac cctcaagatg atcaatcgac
     2581 tgatcgagcc gaccgggggg cgtctcgagg tcctgggcgc cgagatccgg gagatcccgg
     2641 tccacgagct gcgccgccag atcggctacg tcatccagca gatcggcctc ttcccgcaca
     2701 agaccatcgc cgagaacatc gccaccgtgc ccaagctcct gggctgggag aaggcccgga
     2761 cccgccagcg ggtggacgag ctgaccgagc tggtcggcct cgacccggag cacctgaggc
     2821 gctacccggc ggccctgtcg ggcggccagc agcagcgcgt cggcgtcgtg cgcgcgctcg
     2881 cagccgatcc cccggtgctc ctgatggacg agcccttcgg agccgtcgac cccatcgttc
     2941 gggcccggct ccagcgagag ctgctcgacc tgcaggagaa ggtgcgcaag acgatcgtcc
     3001 tggtgaccca cgaca
//
LOCUS       DASTFV010000045         4525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_155784, whole genome shotgun sequence.
ACCESSION   DASTFV010000045 DASTFV010000000
VERSION     DASTFV010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155784"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1526
                     /gene="dnaK"
                     /locus_tag="VFP02_00810"
     CDS             <1..1526
                     /gene="dnaK"
                     /locus_tag="VFP02_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET9541590.1"
                     /translation="VNQAVITVPAYFDDAQRTATQEAGQIAGLEVLRIINEPTAAALA
                     YGMDKEGEDQTILVFDLGGGTFDVSLLDIGEGVFEVKATHGDTNLGGDDWDQRVMDWL
                     VESFKGDHGVDLGKDKMAEQRLKEAAEKAKVELSSTQQAQINLPFITATDSGPLHLDY
                     TLSRAKFQELTADLLERCRGPFEQAINDAGLKTEDIDHVIMVGGSTRMPAVADLVKSI
                     TGKEPHKGVNPDEVVAIGASVQAGVLKGDVKDILLLDVTPLSLGIETKGGVMTKLIER
                     NTTIPTKRTEVFTTADDSQPSVEVHVLQGEREMAQFNKTLGKFQLVDLPPAPRGVPQI
                     EVTFDIDANGIVHVAAKDRATSKEQSMTITGQSSLAKDDIEQMVKDAEAHAEEDRRKK
                     EEAEVRNTADSLVYQAEKQLKEHGDKITGDEKDAVEAKLTELRTALEGTDVEAIKSAT
                     EGLVTASQTFGQRLYDAAAQDAAAAGGGAGPSGPDEPDDDEVVEAEIVDEGDEPQSA"
     gene            1523..2170
                     /locus_tag="VFP02_00815"
     CDS             1523..2170
                     /locus_tag="VFP02_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET9541591.1"
                     /translation="MTKADSPDPGRVVDARPSAPKGKRKGAADPVDEDEAVDGAEPDD
                     AEVVEAEPEASADEAEVEDEVEEAAEVLEADLFAVLATELAAERDDYLDQLRRLQADF
                     ENYRKRVLKQQEETAERATEGLVADLLPVLDACDAAAQHGDDSAGPIAKALVEVLEKQ
                     GLVRLAPEGEAFDPNHHEAVAHEPGEGESVVTEVLRPGYVWKGRVLRPAMVRVRG"
     gene            2174..3304
                     /gene="dnaJ"
                     /locus_tag="VFP02_00820"
     CDS             2174..3304
                     /gene="dnaJ"
                     /locus_tag="VFP02_00820"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET9541592.1"
                     /translation="MDAQREWFEKDYYAVLGVGADASQKELTKAYRKLARENHPDTNK
                     GDAAAEERFKEISAAYDVVGDTDKRAKYDEVRRLGAVGGGFGPGAGGPGGFGGGFGAG
                     AEGVDLGDLLGNLFGGRGRRNHGRPAGPERGVNLETELHLAFEDALAGVTTSVHLVSD
                     AVCSTCHGSGAEPGSDVVVCPQCAGRGVIDENQGLFSFSQPCPRCAGQGMVVSDPCHT
                     CAGSGIERRPRTVKVKIPAGVTDGQRIKVKGRGGPGRNGGPPGDLYVVVHVGRHSLFG
                     RKGRNLTLSVPITFTEAALGADIKVPTLAGEPVTIRIPAGTTSGRTFKVKGKGAVTDA
                     GKGDLLVTVEVAVPQKLSADERTAVEALAEATTESPRAHLGV"
     gene            3306..3686
                     /locus_tag="VFP02_00825"
     CDS             3306..3686
                     /locus_tag="VFP02_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9541593.1"
                     /translation="MAVDRNRAVYVISVAAELAGMHPQTLRIYERKGLLEPARTQGGS
                     RRYSDEDIAMLARIQDLTAEGHNLEGVRRILELEGEIEALQGEIDSLREQAAQAVEDT
                     HRQYRRDLVPVKQSVVLYRGRRRA"
     gene            complement(3711..4454)
                     /gene="yaaA"
                     /locus_tag="VFP02_00830"
     CDS             complement(3711..4454)
                     /gene="yaaA"
                     /locus_tag="VFP02_00830"
                     /inference="COORDINATES: protein motif:HMM:NF015820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HET9541594.1"
                     /translation="MLVVLLPPSEGQAPGGDGPGWAPSDGRFGELARRRTRVVNALKR
                     AKGGTAKALGAKGELLRAAKDANLAAIGASTRPAHERFTGVVWDHLDPPTLTTAGRRR
                     AEVGVLVLSALTGLTAWSDPVPDFRLKLSASLPPVGNLAAFWRPMLSTVLNQHLAGHT
                     VVDLLPNEHRAAWIPDPDRYDLRRPALVFPDGRPAGHGGKAAKGLLARALLLSGDVDR
                     TLATFDPAAQGLVGADDLVLAVDEALTGP"
BASE COUNT          752 a         1604 c         1637 g          532 t
ORIGIN      
        1 ccgtcaacca ggcggtcatc acggtgcccg cctacttcga cgacgcccag cgcaccgcca
       61 cccaggaggc gggccagatc gccggcctcg aggtcctgcg catcatcaac gagccgaccg
      121 cggcggccct ggcctacggc atggacaagg agggcgagga ccagaccatc ctggtgttcg
      181 acctcggcgg cggcaccttc gacgtctccc tgctcgacat cggcgagggc gtcttcgagg
      241 tcaaggccac ccacggtgac accaacctcg gtggggacga ctgggaccag cgggtcatgg
      301 actggctggt cgagtccttc aagggcgacc acggcgtcga cctgggcaag gacaagatgg
      361 ccgagcagcg cctgaaggag gcggccgaga aggccaaggt cgagctctcc agcacccagc
      421 aggcccagat caacctgccc ttcatcaccg ccaccgacag cgggcccctg cacctggact
      481 acacgctcag ccgggccaag ttccaggagc tcacggccga cctgctcgag cggtgccgcg
      541 gccccttcga gcaggccatc aacgacgccg gtctgaagac cgaggacatc gaccacgtca
      601 tcatggtcgg cggctccacc cgcatgcctg ccgtcgccga cctggtcaag agcatcacgg
      661 gcaaggagcc ccacaagggc gtcaaccccg acgaggtcgt ggccatcggc gcctccgtgc
      721 aggccggcgt cctcaagggc gacgtgaagg acatcctgct cctcgacgtg acgccgctgt
      781 ccctcggcat cgagaccaag ggcggggtca tgaccaagct catcgagcgg aacacgacca
      841 tccccaccaa gcggaccgag gtcttcacca cggccgacga cagccagccg tcggtcgagg
      901 tccacgtcct gcagggcgag cgggagatgg cccagttcaa caagaccctg ggcaagttcc
      961 agctggtcga cctgcccccg gccccgcgcg gcgtgcccca gatcgaggtc acgttcgaca
     1021 tcgacgccaa cggcatcgtc cacgtggcgg ccaaggaccg ggcgacctcc aaggagcagt
     1081 cgatgacgat cacgggccag tcgtcgctgg ccaaggacga catcgagcag atggtcaagg
     1141 acgccgaggc ccacgccgag gaggaccgcc gcaagaagga ggaggccgag gtccgcaaca
     1201 cggccgactc gctcgtctac caggccgaga agcagctcaa ggagcacggc gacaagatca
     1261 cgggcgacga gaaggacgcg gtcgaggcca agctgaccga gctgcggacg gccctggagg
     1321 gcaccgacgt ggaggccatc aagagcgcca ccgaggggct cgtgaccgcc agccagacct
     1381 tcggccagcg tctctatgac gccgccgccc aggacgccgc ggccgccggc ggtggggccg
     1441 gtccctccgg gccggacgag cccgatgacg acgaggtcgt cgaggccgag atcgtcgacg
     1501 agggcgacga gccccagtcg gcatgaccaa ggccgactcg ccggacccgg gtcgggtcgt
     1561 ggacgcccgc ccgagcgcgc ccaagggcaa gcgcaagggc gcggccgacc cggtcgacga
     1621 ggacgaggcg gtcgacggag ccgagcccga tgacgccgag gtcgtcgagg ccgaacccga
     1681 ggcgtccgcc gacgaggccg aggtcgagga cgaggtcgaa gaggcggccg aggtcctcga
     1741 ggccgacctc ttcgcggtcc tggccaccga gctggccgcc gagcgcgacg actacctgga
     1801 ccagctgcga cggctccagg ccgacttcga gaactaccgc aagcgggtgc tcaagcagca
     1861 ggaggagacc gccgagcggg ccaccgaagg cctggtcgcc gacctcctgc ccgtgctcga
     1921 cgcctgtgac gccgccgccc agcacggtga cgactccgcc ggtcccattg cgaaggcctt
     1981 ggtcgaggtc ctcgagaagc agggcctggt gaggctggcc cccgagggcg aagccttcga
     2041 ccccaaccac cacgaggccg tggcccatga gccgggcgag ggagagtcgg tcgtgaccga
     2101 ggtcctgcgc cccgggtacg tctggaaggg ccgcgtcctg cggcccgcca tggtgcgagt
     2161 gcgggggtag cccgtggacg cccagcggga gtggttcgag aaggactact acgcggtcct
     2221 gggcgtgggc gcggacgcca gccagaagga actcaccaag gcgtaccgca agctggcccg
     2281 cgagaaccac ccggacacca acaagggtga cgccgcggcc gaggagcgct tcaaggagat
     2341 ctccgcggcg tacgacgtcg tcggcgacac cgacaagcgg gccaagtacg acgaagtcag
     2401 gcgcctcggc gcggtcgggg gcggcttcgg tcccggtgcc ggcggccctg gcggcttcgg
     2461 gggcggcttc ggcgcgggcg ccgagggcgt cgacttgggt gacctactgg gcaacctctt
     2521 cggcggtcgg ggccgccgca accacggccg tcccgccggg cccgagcgcg gcgtcaacct
     2581 ggagaccgaa ctgcacctgg ccttcgagga cgcgctggcg ggggtcacca cctcggtgca
     2641 cctggtcagc gacgccgtgt gctccacctg tcacggcagt ggtgcggagc cgggcagcga
     2701 cgtggtcgtc tgcccccagt gcgccggccg gggcgtcatc gacgagaacc aaggcctgtt
     2761 ctccttcagc caaccgtgcc cgcgctgcgc cggccagggc atggtcgtca gcgacccgtg
     2821 ccacacctgt gccgggtcgg gcatcgagcg ccggccgcgc accgtcaagg tcaagatccc
     2881 cgccggtgtc accgacggtc agcggatcaa ggtcaagggt cgcggcggcc ccggccgcaa
     2941 cggcggccca cccggcgacc tgtacgtggt cgtgcacgtc gggcggcatt cgctgttcgg
     3001 ccgcaaaggc cgcaacctca cgctgagcgt gcccatcacc ttcacggagg cggccctcgg
     3061 ggccgacatc aaggtcccga ccctggcggg ggagcccgtc accatccgca tcccggccgg
     3121 cacgaccagc gggcgcacgt tcaaggtgaa gggcaagggg gcggtgaccg acgcgggcaa
     3181 gggcgatctc ctcgtcaccg tcgaggtcgc cgtgccgcag aagctgtcgg ccgacgagcg
     3241 cacggcggtc gaagcactgg cggaggcgac caccgagtcg ccccgagcgc atctgggggt
     3301 gtgacatggc cgtggaccgg aatcgagccg tctacgtgat ctcggtggcg gccgagctgg
     3361 cggggatgca cccgcagacc ctgcgcatct acgagcgcaa gggcctcctg gagccggcgc
     3421 gcacccaagg aggcagccgt cgctacagcg acgaggacat cgccatgctg gcccgcatcc
     3481 aggacctcac cgccgaaggc cacaacctcg agggggtgcg ccggatcctg gagctggagg
     3541 gcgagatcga ggccctgcag ggtgagatcg actcgttgcg cgagcaggcg gcccaggcgg
     3601 tcgaggacac ccatcgtcag taccgccgcg acctggtccc ggtgaagcag tccgtcgtcc
     3661 tgtatcgggg gcgccgccgc gcctgagccc ggcccggcgt cgtccggacg tcaggggccg
     3721 gtcagcgcct catcgacggc gaggacgagg tcgtccgccc cgaccaggcc ctgagccgcc
     3781 gggtcgaagg tggccagggt ccggtccacg tcgccggaca ggaggagcgc tcgggccagc
     3841 aggcccttgg ccgccttgcc cccgtggcct gcgggccggc cgtcggggaa caccagggcc
     3901 ggacggcgca ggtcgtagcg gtccgggtcg gggatccacg ccgcccggtg ctcgttgggc
     3961 aggaggtcga cgaccgtgtg gccggccagg tgctggttga gcacggtgga gagcatcggc
     4021 cgccagaacg cggccaggtt gcccacgggt ggcagggacg ccgagagctt cagccggaag
     4081 tcgggcaccg ggtcgctcca ggcggtgagg ccggtgagcg ccgacaggac gaggacgccc
     4141 acctccgccc gccggcggcc ggccgtggtc aacgtcgggg gatcgaggtg gtcccacacg
     4201 acgccggtga accgttcgtg ggccggccgg gtcgatgcgc cgatggcggc gaggttggca
     4261 tccttggccg cccggagcag ttcccccttg gccccgaggg ccttcgccgt cccgcccttg
     4321 gcccgcttca gggcgttgac gacccgagtc cgtcgccggg cgagctcgcc gaaccgcccg
     4381 tcggacggtg cccaccccgg gccgtccccg ccgggggcct gtccttcgga cggcggcagc
     4441 agcacgacga gcacggccgc agatccggcc cgctcgaccg aggtcgtcgc acatgacgcc
     4501 ccgacctagg ccagtgccgt cgttc
//
LOCUS       DASTFV010000046         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_160133, whole genome shotgun sequence.
ACCESSION   DASTFV010000046 DASTFV010000000
VERSION     DASTFV010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160133"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VFP02_00835"
     CDS             <1..874
                     /locus_tag="VFP02_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9541595.1"
                     /translation="VVGGGYIGLEMAESYLRLGAKVTVVDRAPEVMGTLDPDMAALVR
                     TAMVRHGIDVRCGSPVDAFEPGSVHLESGDTLNADLVVLGMGVEPNSQLAADAGVETG
                     VKGAIRVNARLQTSVDGIWAAGDCTESFHLVSRKGVHLPLGTVANRQARVAGINLGGG
                     YATFPGVVGTAVTKLCATEVARTGLNEREAAAAGLGYVVGKIKAKTQAGYLPEARPIV
                     VKMLAERGTGRILGAQLVGEEGAGKRIDAVAVALSAGFTVEDLVNADLAYAPPFSPLW
                     DPIQVAARKALALL"
     gene            876..>2264
                     /locus_tag="VFP02_00840"
     CDS             876..>2264
                     /locus_tag="VFP02_00840"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9541596.1"
                     /translation="MTRPVLDREALLDDPTIAGRDFCAAYTAAVDDWLRALFAEAVPD
                     AKGLALVAVGGYGRAELAPGSDLDLLLLHAPRRLPGGAAQALWYPIWDAGLKLGHAVR
                     TVKQALALAAEDLDTATSLLTCRYLAGDRALSDDLAEQAVALWRKRSKRWLAQVAESV
                     HERHRQHGEVAFLLEPDLKEGRGGLRDVHALGWSEAARPQLTRGDPEALASAGDTLLA
                     VRVELHRRTRRPSDRLVLQEQDAVAEALGYEGADALMADVSRAARTVAWIADEAWGRT
                     ESALAGPTGRLARSDRQLGPGVILRDGEVHLEADADPALVPALVLRVGVAAAQHDARI
                     DRSSLDRLAAEVPPFGDPWPEGARGLLADLLLAGRPAIRVLETLDQRGLLVRILPEWE
                     PVRSRPQRNAYHQFTVDRHLWEAAVNAAALADSVHRHDLLVIGALLHDIGKGYPGDHT
                     DVGIDLVERIGPR"
BASE COUNT          290 a          868 c          801 g          305 t
ORIGIN      
        1 cgtcgtgggc gggggctaca tcggcctgga gatggccgag tcctacctgc ggctcggcgc
       61 caaggtcacc gttgtcgacc gggcacccga ggtcatgggc accctcgacc cggacatggc
      121 ggcgctggtg cgcaccgcca tggtccgcca cggaatcgac gtccgttgcg gctcaccggt
      181 ggacgccttc gagcccggct cggtccacct ggaatccggt gacaccttga acgccgacct
      241 cgtcgtgctg ggcatggggg tcgagcccaa cagccaactg gccgccgacg ccggcgtcga
      301 gaccggcgtc aagggtgcca tccgggtcaa cgcccgccta cagaccagcg tcgacgggat
      361 ctgggcggca ggcgactgca ccgagtcgtt ccacctggtc tcccgcaagg gcgtccacct
      421 ccccctcggg accgtggcca accgccaggc tcgggtggcc ggcataaacc tgggcggcgg
      481 ttacgccacg ttccccggcg tggtcggcac ggcggtgacg aagctctgtg ccaccgaggt
      541 ggcccgcacc ggcctcaacg agcgcgaggc ggccgccgcc ggcctgggct acgtcgtggg
      601 caagatcaag gccaagacgc aggccggcta tctgcccgag gcccggccca tcgtggtgaa
      661 gatgctggcc gagcgcggaa ccggtcgcat cctgggggcc cagctggtcg gtgaggaggg
      721 ggccggcaag cgcatcgacg ccgtggctgt cgccctcagc gccggcttca ccgtggagga
      781 cctggtcaat gccgacctgg cctacgcccc gcccttctcc cccctgtggg acccgatcca
      841 ggtggcggcc cgcaaggccc tcgccctcct gtgacttgac tcgccccgtc ctcgaccgcg
      901 aggcgctcct cgacgacccc acgatcgccg gtcgggactt ctgtgcggcc tacacggccg
      961 cggtcgacga ctggctgcgg gccctgttcg ccgaggcggt cccggacgcc aaggggctcg
     1021 ccctcgtggc ggtcgggggc tacggccggg ccgagctggc tccgggaagc gacctcgacc
     1081 tcctcctgct gcacgcgccg cgccggctgc cggggggcgc ggcccaggcc ctctggtacc
     1141 ccatctggga cgcgggcctg aagctgggcc acgccgtgcg cacggtgaag caggccctcg
     1201 ccctcgcggc cgaggacctg gacacggcca cctcgctcct gacctgtcgc tacctggccg
     1261 gcgaccgggc gctctccgac gacctggccg agcaggccgt ggcgctctgg cgcaagcgct
     1321 ccaagcgctg gctggcccag gtggcggagt cggtgcacga gcgccaccgc cagcacggcg
     1381 aggtggcctt cctcctcgag cccgacctga aggagggccg gggtgggctg cgggacgtcc
     1441 acgccctcgg ttggtccgaa gccgctcgcc cgcagctgac caggggcgat cccgaggccc
     1501 tggcctccgc cggcgacacc ctcctcgcgg tacgggtgga gctccaccgc cggacgcgcc
     1561 ggcccagcga ccgcctcgtg ctgcaggagc aggacgcggt ggccgaggcc ctcggctacg
     1621 agggggccga cgcgctcatg gccgacgtgt cccgggcggc ccgcacggtg gcctggatcg
     1681 cagacgaggc ctggggccgg accgagtccg cactggccgg tcccaccggc cgactggccc
     1741 gctcagaccg tcagctcgga cccggtgtga tcctgcgcga cggcgaggtc cacctcgagg
     1801 ccgacgccga tccggccctg gtgcccgccc tcgtcctccg ggtgggggtc gccgccgctc
     1861 agcacgacgc ccgcatcgac cggtcatcgc tggaccgcct ggccgccgag gtccctccgt
     1921 tcggggaccc gtggcccgaa ggggcccgtg gactgctggc cgacctcctg ctcgccggtc
     1981 gtcccgccat tcgcgtcctg gagaccctcg accagcgcgg tctgctggtg cggatcctcc
     2041 cggagtggga gccggtgcgc agccgtccgc agcgcaacgc ctaccaccag ttcacggtcg
     2101 accggcacct ctgggaggcc gcggtcaacg cggccgccct ggccgacagc gtgcaccgcc
     2161 acgacctgct cgtcatcggc gccctcctgc atgacatcgg caagggctac cccggtgacc
     2221 acaccgacgt cggcatcgac ctggtggagc gcatcggtcc tcgc
//
LOCUS       DASTFV010000047         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_160270, whole genome shotgun sequence.
ACCESSION   DASTFV010000047 DASTFV010000000
VERSION     DASTFV010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160270"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VFP02_00845"
     CDS             complement(<1..580)
                     /locus_tag="VFP02_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9541597.1"
                     /translation="MGGRLDGKVAIVTGAARGQGEAETRLFVAEGAHVVATDVLTDAV
                     EAVAADLGDGAIALTHDVADADAWASVVAAAMDRFGGVDVLVNNAGIHWIRAMVDEDP
                     ADLERLLRVNLVGPYIGMQAVIPAMQERGGGSIVNISSFAGLAGAWGHSAYGASKWGL
                     RGITKTAAIELGPLGIRVNSVHPGPIDTAMLPV"
     gene            611..1342
                     /locus_tag="VFP02_00850"
     CDS             611..1342
                     /locus_tag="VFP02_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET9541598.1"
                     /translation="MNFEPTADRLLPELDIRTEMVLLARTLWREGYDDHLAGHITVNQ
                     GDGTLLCNPWLLTWEEFRPQDVIRIDLEGNVVEGDWPVPLGIPLHLELHRARPDVQVA
                     VHHHPRWGTVWADLGRLPGRHDQSSVLGGGTAALVDEYGGAVNDPSSAAKAIAAMGDA
                     DLAILAHHGVFILGGSIRAAHQRAVAFESRCRTAWLTEVGGGGEELPPAVLGFFESGD
                     GEGFRGFWEAAARRELRADPSLLPD"
     gene            1417..2142
                     /locus_tag="VFP02_00855"
     CDS             1417..2142
                     /locus_tag="VFP02_00855"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9541599.1"
                     /translation="MTTALLIHGGASTARFWDRVVDRVGRAAVAVDLPGRRDRPADLM
                     TVTLDDGAASVAADLAAASPEGPVVVVAHSSGGLVVPRVVDRLGDRVTAVVLSSASVP
                     PEGGNGLDCMKASHRERMVAGIEWARAEGRRLVAPVPDDPEALRESYGERLDDDTLAF
                     VFERLVEDSFNVYFDPVTWSTVTAPITYVRASLDRAVPPALQDEMIAHLPGAPTVLDW
                     DCGHIPPITRPDPFSALLREFLN"
     gene            2155..>2621
                     /locus_tag="VFP02_00860"
     CDS             2155..>2621
                     /locus_tag="VFP02_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9541600.1"
                     /translation="MGSWIGQYCINVTDLDATVVFYEALGLECTSRTRIDTAGEAIVE
                     TVGKGGKVQFAEQDDQDGPLDMGNAFWKLYVNSNDIGVTHAAALAAGAEEVMAPMRME
                     QWPTSISFVKDGDGYLVELVQRHPWLDGDDTTPAWLGQYCINVTDLEATIAFY"
BASE COUNT          367 a          945 c          930 g          379 t
ORIGIN      
        1 gcacgggcag catcgcggtg tcgatggggc cggggtgcac cgagttgacc cggatgccca
       61 gagggcccag ctcgatggcc gccgtcttgg tgatgccccg cagcccccac ttcgacgcgc
      121 cgtacgcgct gtggccccag gccccggcca gcccggcgaa gctcgagatg ttgacgatgg
      181 agccgccgcc ccgctcctgc atcgccggga tcacggcctg catcccgatg taggggccga
      241 ccaggttcac ccgcagcagg cgctcgaggt cggccgggtc ctcgtccacc atcgcccgga
      301 tccagtggat gccggcgttg ttgacgagca cgtcgacccc gccgaagcgg tccatggcgg
      361 cggcgacgac ggaggcccag gcgtcggcgt cggccacgtc gtgggtcagc gcgatggccc
      421 cgtcccccag gtcggcggcc acggcctcga ccgcgtcggt cagcacgtcg gtggccacca
      481 cgtgggctcc ttcggcgacg aacagccggg tctcggcctc accctggccc cgcgccgctc
      541 ccgtgacgat ggcgaccttg ccgtccagac gaccccccac gcgcggtgaa tctagactca
      601 atccgctgac atgaacttcg aaccgaccgc cgaccggctg ctgccggagc tggacatccg
      661 caccgagatg gtgctgctgg cccgcaccct ctggcgggag ggctacgacg accacctggc
      721 cggccacatc acggtcaacc agggcgacgg cacgctgctc tgcaacccgt ggctcctgac
      781 ctgggaggag ttccggcccc aggacgtcat ccgcatcgac ctcgagggca acgtggtcga
      841 gggcgactgg ccggtgccgc tgggcatccc gctgcacctc gagctgcacc gggctcggcc
      901 cgatgtccag gtcgccgtcc accaccaccc ccgctggggc acggtctggg ccgacctcgg
      961 tcgcctgcct ggtcgccacg accagagctc ggtcctgggc gggggcacgg cggcgctggt
     1021 cgacgagtac ggcggcgccg tcaacgaccc ctcgtcggcg gccaaggcca tcgccgccat
     1081 gggcgacgcc gacctggcca tcctggccca ccacggcgtg ttcatcctgg gcggctccat
     1141 ccgggccgcc caccagcggg ccgtggcctt cgagtcccgt tgccgcacgg cgtggctgac
     1201 cgaggtcggc ggtggcggcg aagagttgcc cccggctgtc ctgggcttct tcgagagcgg
     1261 cgacggcgag ggcttccgcg gcttctggga agcggcggcc cgccgcgagc tccgagccga
     1321 tcccagcctc ctgcccgact gaggtcggtc atgacccgct ccgcggtgct ggagcctttc
     1381 gttcgtggtg ggccggacgg ggggtgaggg cgggagatga cgacggcgct gctgatccat
     1441 ggcggggcgt cgacggcgcg cttctgggac cgggtggtgg accgggtcgg acgggcggcc
     1501 gtcgccgtcg acctgcccgg acggagggac cggccggcgg acctgatgac ggtgaccttg
     1561 gacgacgggg cggcatcggt tgcggccgac ttggcggcgg cgtcccccga gggaccggtc
     1621 gtggtcgtgg cccactcgtc aggcgggctg gtcgtgcccc gcgtcgtcga ccgtctcggg
     1681 gaccgggtca cggccgtggt cctctcgtcc gcctcggtcc cgccggaggg gggcaacggg
     1741 ttggactgca tgaaggccag ccaccgggag cggatggtgg ccggcatcga gtgggctcgg
     1801 gccgagggcc ggcgcctggt cgcgcccgtg cccgacgatc ctgaagccct acgggagtcc
     1861 tacggcgagc gcctcgacga cgacacgttg gccttcgtct tcgagcggct ggtcgaggac
     1921 tcgttcaacg tctacttcga cccggtgacc tggtcgacgg tgacggcgcc gatcacgtac
     1981 gtacgcgcca gcctcgaccg ggccgtcccc ccggcgctgc aggacgagat gatcgcccac
     2041 ctcccgggcg ccccgaccgt cctggactgg gactgcggcc acatcccccc gatcacccgt
     2101 cctgatccgt tctcggcctt gctccgggag tttctgaact agagtagaaa actcatgggg
     2161 agctggatcg ggcagtactg catcaacgtc accgacttgg acgcgacggt cgtcttctac
     2221 gaggccctcg gcctcgagtg caccagccgt acccggatcg acaccgcggg cgaggccatc
     2281 gtcgagacgg tgggcaaggg cggcaaggtg cagttcgccg agcaggacga ccaggacggc
     2341 ccgctcgaca tgggcaacgc cttctggaag ctctacgtga acagcaacga catcggcgtc
     2401 acccacgccg ccgccctcgc cgccggggcc gaggaggtca tggccccgat gcggatggag
     2461 cagtggccga ccagcatcag cttcgtgaag gacggcgacg gctacctggt cgagctcgtc
     2521 cagcgccacc cctggctcga cggggacgac accacgccgg cgtggctcgg ccagtactgc
     2581 atcaacgtca cggacctcga ggccacgatc gccttctacg a
//
LOCUS       DASTFV010000048         2776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_160478, whole genome shotgun sequence.
ACCESSION   DASTFV010000048 DASTFV010000000
VERSION     DASTFV010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160478"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VFP02_00865"
     CDS             <1..382
                     /locus_tag="VFP02_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541601.1"
                     /translation="VVGGGLAVLAARSLHALSRRFLVFVPGGVVVHDPMTIVEPVLLP
                     KGSLASIGPAPADTDAEDLTMGAGGLLMELRLQQPADLLRRRPGRDPAEVVSTAAVLV
                     APTRTAAVLAEAESRHLPLDRPRS"
     gene            432..1073
                     /locus_tag="VFP02_00870"
     CDS             432..1073
                     /locus_tag="VFP02_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541602.1"
                     /translation="MSVTPPAAPWSPPPPPLPARRRRWPWVVFGLVVGLPVLAMTTFV
                     GMWYLALQGPRPTGADQERLDVLQAESILDRAPEGGQLLSDEAEAGECCGPFFNTSED
                     TRAVRTWALDEDDHLSSLRDFARAAADDGWTVDVPDNKRTSTCDRVEARKDFGDWTGH
                     LTVTADPWDGELEVALSTPAAEGASRRSTTLPGGYQRDGVTLDEAEALARLCV"
     gene            complement(1070..2098)
                     /gene="mnmA"
                     /locus_tag="VFP02_00875"
     CDS             complement(1070..2098)
                     /gene="mnmA"
                     /locus_tag="VFP02_00875"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET9541603.1"
                     /translation="MRVLCAMSGGVDSSVTAALLAEQGHAVQGVTLRLWGGESDQGCC
                     SVADVDDARRVADHLGLDHHVFNFGPDFDRQVVAPYVAAHAEGRTPNPCVECNRHLKF
                     DRLLDRARALGFDAIATGHHARVVERPDGTRRVARGADGPKDQSYVLYMLDQAALAQV
                     LLPVGTMTKAEVRDRAAALGLRTAAKPDSQDVCFIQAAAGRRGFLESRIDLTPGRIVD
                     ADGRKVGEVDAVELVTVGQRRGLGLRSQQEPSFVTSVDVPTATVTMGRKEDLLGQELV
                     LTDLVWADRPVAGPVLAQCSAHGEVRSGVLDGSRLRWDQPQPRVAPGQSVAFYDGAEV
                     LGGGIASV"
     gene            complement(2095..>2776)
                     /locus_tag="VFP02_00880"
     CDS             complement(2095..>2776)
                     /locus_tag="VFP02_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9541604.1"
                     /translation="VAEVVRRHAPDAVLHTDAVQALSWVDVATAAAGADLISVSAHKF
                     GGPKGVGALVVRPGVRLAARQIGGGQERDRRSGTQNVAGVAAMATAAGLTAARRTADT
                     ARVAALRDRLVDGLLAEVPDAVETGIAAGDRSGRAAGLAHVCFPGVEAEALLFLLDQA
                     GVAASAASSCASGAAEPSHVLAAMGVPRDQAAGSLRLSLGWASTEADVDRALEVVPGA
                     VTQLRAAR"
BASE COUNT          376 a         1039 c         1007 g          354 t
ORIGIN      
        1 ggtcgtgggc ggcgggctgg ccgtcctcgc cgctcgatcg ctgcacgccc tgtcccgccg
       61 cttcctggtc ttcgtgcccg gcggcgtcgt cgtgcacgac ccgatgacca tcgtggagcc
      121 cgtcctgctg cccaagggct cgctggcctc gatcggcccg gcgccggccg acaccgacgc
      181 cgaggacctg accatgggcg ccggcggcct gctcatggag ctccgcctcc agcaaccggc
      241 ggacctgctc cggcgccgcc ccggccgcga cccggccgag gtggtcagca ccgctgccgt
      301 cctcgtcgcc cccacccgca ccgccgccgt cctcgccgaa gccgagtccc gccacctccc
      361 cctcgaccgc cctcgctcct gatccagatc gtgacgactc gacccggtca ttcatcggcc
      421 gacggaggtg cgtgagcgtc acgccacccg ccgcaccgtg gtcgccgcct ccacctccgc
      481 tgcccgcccg ccgccggcgt tggccgtggg tcgtcttcgg cctcgtcgtc gggctgccgg
      541 tcctggccat gaccaccttc gtcgggatgt ggtacctcgc cttgcaaggt ccgcgcccga
      601 ccggtgccga ccaggagcgc ctcgacgtgc tccaggcgga gtcgatcctc gaccgggcac
      661 cggagggcgg ccagttgctc tccgacgagg ccgaagccgg cgaatgctgt gggccgttct
      721 tcaacaccag cgaggacacg cgcgccgtga ggacgtgggc tctcgacgag gacgatcatc
      781 tgtcgtcgct ccgggacttc gctcgcgccg cggcggatga cggctggacg gtcgatgtcc
      841 cggacaacaa acggacctca acctgcgacc gggtcgaggc aaggaaggac ttcggtgact
      901 ggacaggcca tctgacggtc accgccgatc cgtgggacgg tgagctggag gtggcactga
      961 gcacccccgc cgcggagggc gctagtcggc gttcgacgac gctgccgggc ggctaccaga
     1021 gggacggcgt cacactggac gaggccgaag ctctggctcg gctctgcgtc tagaccgagg
     1081 caataccgcc gcccagcacc tccgctccgt cgtagaaggc cacgctctga cccggggcga
     1141 cccggggctg cggctggtcc caacggagcc gcgagccgtc gaggacgccc gagcggacct
     1201 caccgtgggc gctgcactgg gccagcacgg ggccggcaac gggccggtcg gcccagacca
     1261 ggtcggtcag gaccagctcc tggccgagca ggtcctcctt gcgacccatg gtcaccgtcg
     1321 ccgtcggcac gtccaccgag gtcacgaagc tcggttcctg ctggctgcgc aggccgaggc
     1381 cccggcgctg gccgaccgtg accagctcga cggcgtcgac ctcgccgacc ttccggccgt
     1441 cggcatccac aatccgtcca ggggtcaggt cgatgcggga ctcgaggaag ccccggcgcc
     1501 cggccgccgc ctggatgaag cagacatcct ggctgtcggg cttggccgcc gtgcgcaggc
     1561 cgagggccgc ggcccggtcc cggacctccg ccttggtcat ggtgccgacc ggcagcagga
     1621 cctgggccag cgcggcctgg tcgagcatgt agagcacgta ggactggtcc ttggggccgt
     1681 cagcgccccg ggcgacgcga cgggtgccat cgggccgctc gacgactcgg gcgtgatggc
     1741 cggtggcgat ggcgtcgaag ccgagggccc gggcccggtc caggagccgg tcgaacttca
     1801 ggtgccggtt gcactccacg caggggttgg gcgtccgccc ctcggcgtgg gcggcgacgt
     1861 agggggccac gacctggcgg tcgaagtcgg gcccgaagtt gaagacgtgg tggtcgaggc
     1921 cgaggtggtc ggccactcgg cgggcgtcgt cgacgtcggc caccgagcaa cagccctggt
     1981 cggactcacc gccccacaga cggagggtga cgccctggac ggcgtggccc tgctcggcca
     2041 ggagcgcggc ggtgaccgac gagtccactc cgcccgacat cgcgcagagg accctcatcg
     2101 ggccgcccgc agctgggtca cggcgcccgg cacgacctcg agggcccggt ccacgtccgc
     2161 ctcggtgctg gcccagccca acgagaggcg cagcgagccc gcagcctggt cccggggcac
     2221 acccatcgcg gccaggacgt gggacggctc ggccgcgcca ctggcacagg acgaggcggc
     2281 ggaggcggcc accccagcct ggtcgagcag gaagagcagg gcctccgcct ccacccccgg
     2341 gaagcagacg tgggccaggc cggcggcgcg gccagaacgg tcgccggcgg cgatgccggt
     2401 ctccaccgcg tccgggacct cggccaggag cccgtcgacc agacggtcac ggagggcggc
     2461 gacgcgggcc gtgtccgcgg tccggcgggc ggcggtcagg cccgccgccg tggccatggc
     2521 ggcaacgccg gcgacgttct gcgtaccact gcgtcggtcc cgctcctggc ccccaccgat
     2581 ctgacgggcg gccagcctca caccgggacg cacgacgagg gccccgacgc ccttggggcc
     2641 gccgaacttg tgggcgctga ccgagatgag gtctgcacct gcggcagctg tggccacgtc
     2701 gacccaggac agggcctgga cggcgtcggt gtggagcacg gcgtcggggg catggcgacg
     2761 gacgacctca gccacc
//
LOCUS       DASTFV010000049         2808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_165736, whole genome shotgun sequence.
ACCESSION   DASTFV010000049 DASTFV010000000
VERSION     DASTFV010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165736"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFP02_00885"
     CDS             complement(<1..656)
                     /locus_tag="VFP02_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HET9541605.1"
                     /translation="MDRQGGGIEDLWARYRDDATRPDRDQLIVHYSPLVKYVAGRVAV
                     GLPQNVEHADLVSYGMFGLIDAIDKFDPERGFKFETYAISRIKGAILDELRSIDWVPR
                     SVRAKARALERSYAKLEAEHARPPTDLELADALEMTEIQLQTLLTQISFVGMAALDEV
                     LAMGGERGEAVTLGDTIADSGDGPVSRFEVEETRQLLASTINRLPEREKLVLTLYYYE
                     G"
     gene            complement(666..1502)
                     /locus_tag="VFP02_00890"
                     /pseudo
     CDS             complement(666..1502)
                     /locus_tag="VFP02_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
     gene            1669..>2808
                     /locus_tag="VFP02_00895"
     CDS             1669..>2808
                     /locus_tag="VFP02_00895"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HET9541606.1"
                     /translation="MPLRTADEYRAGLVDGRRLYYQGRPVADINQEPDLKIAVDHAAI
                     DFDLAHDPDHRDLAVVTDPDTGEEYNAAYRIPRNAEDVMARSKLIEAGTAAGGTVVTL
                     IHEIGTDGMFAMLRVLEGEGRERAEAFYRHCRDGDLAVAVAQTDVKGDRSKPPHEQED
                     PDLYVRVVDRSDEGIVVRGAKCHTTSSANGDEIIVFPTRAMGPDDADYAVSFAVPANT
                     EGLSLYVSGYGGGGGGHDAFDHPVSSRHKLLETLTVFDDVFVPWERVFLCQEPEKAGA
                     LALTFVEYHRFTAVSYKLPLVDAFVGAAAQIAEMNGVMKAGHIRDKLTHLVAYAETVR
                     ALTEAAGLRSRIGEHGIAYPDPMTTNMAKFTFASGFYKAVEWLQDI"
BASE COUNT          424 a          964 c         1031 g          389 t
ORIGIN      
        1 ccttcgtagt agtagagggt caggaccagc ttctcccgct ccggcaggcg gttgatggtc
       61 gaggccagca gctggcgggt ctcctcgacc tcgaagcggc tgaccggacc gtcgccgctg
      121 tcggcgatcg tgtcgcccag cgtgacggcc tcgcctcgct cgccccccat ggccagcacc
      181 tcgtcgaggg cggccatgcc gacgaacgag atctgggtga ggagggtctg gagctggatc
      241 tcggtcatct ccagggcgtc ggccagctcg aggtcggtcg gggggcgggc gtgctcggcc
      301 tcgagcttgg cgtaggaccg ttcgagggcc cgggccttgg cccgcaccga tcgagggacc
      361 cagtcgatcg agcggagctc gtcgaggatg gcgcccttga tccggctgat ggcgtaggtc
      421 tcgaacttga agcctcgctc gggatcgaac ttgtcgatgg cgtcgatgag gccgaacatc
      481 ccgtagctca cgaggtcggc gtgctcgacg ttctggggca ggcccaccgc cacccggccg
      541 gcgacgtact tcaccagcgg tgagtagtgg acgatcagct ggtcccggtc gggccgcgtg
      601 gcgtcgtcgc ggtaccgggc ccagaggtcc tcgattccac cgccttgtcg atccaccgcc
      661 cgtccctacg ctcgaggatg cgtcgtctcg agcacgccgc gcaggcgttc ccggctcaca
      721 tgggtgtaga gctgggtggt cgcgagatcg gcgtgtccga gtagttcctg tacagcacga
      781 aggtcggcac cgccgtccag aagatgagtg gcgaaggtgt ggcggagggc atggggatga
      841 gccggaccgg ccgcccgtcg gtcgaggatg cggcgtacgt cgcggggcgt gagggggtgg
      901 gcccgccggt tcacgaagac cacgtcgtcc ggcgtgtcgg cgtcggccag caccgcacga
      961 ccctggtcca gccactggcg gagggcgtcg accgccggtg ccgagagggg caccgtgcgc
     1021 tgcttggacc ccttgcccca gacggtcacg cggtgccggg gcaggtcgat gtcgaccggc
     1081 cgaaggccac aggcctcagc cacccggaga ccgctgccgt agaggatctc caggacggcg
     1141 cggtcccgct gggcgagggc ggcttcgacg gggtcgtcgg tgggcggcgg gccatcgtcg
     1201 aggagggcct cgatctcgtc ccggcgcagg acccggggca ggcggccgtc gtgaccggga
     1261 gccgacagtc gggccgaggg gtcgacctcg atgcgccccc gtcggcggag ccagtcgaag
     1321 tagcggcgca gcgaggcgac gtggcgcgcg atcgtgcggc gggcatagcc ccgggtggtg
     1381 aggaaggcga cgtagcgccg caaggcccgc cggtcgacgg cggtcgggtc ctcgaggccg
     1441 aggcgaccgg cccactccac gaaggcgtcc aggtcccggc ggtaggcggt gacgctggcc
     1501 ggggccaaag cggtcaggga cgtcgtgaac tcatccaggt cccaggccac gggcgcacgg
     1561 tagccccggc gtcacccaga gggcacgacc ccgcgcggag ggtggcagga tcgaccgcgg
     1621 gtgccgaccg tgagagccga ccggccgccc tcacctacca tcgcctgcat gcccttgcgc
     1681 accgcggacg agtaccgggc gggactggtc gacggccggc gcctgtacta ccaggggcgg
     1741 cccgtcgccg acatcaacca ggagccggac ctgaagatcg cggtggacca tgcggcgatc
     1801 gacttcgacc tggcccacga cccggaccac cgcgacctgg cggtcgtcac cgacccggac
     1861 acgggcgagg agtacaacgc cgcctaccgc atcccccgca acgcggagga cgtgatggcc
     1921 cggtccaagc tgatcgaggc ggggaccgcg gccggcggca cggtcgtcac cctgatccac
     1981 gagatcggca ccgacggcat gttcgcgatg cttcgcgtcc tcgaggggga gggccgggag
     2041 cgggccgagg ccttctaccg gcactgccgg gacggcgacc tggccgtggc cgtggcccag
     2101 accgacgtca agggcgaccg atccaagccg ccgcacgagc aggaggaccc cgacctctac
     2161 gtccgggtgg tcgaccgcag cgacgagggc atcgtcgtgc ggggggccaa gtgccacacc
     2221 acgagcagcg ccaacggcga cgagatcatc gtcttcccca cccgggccat gggccccgac
     2281 gacgccgact acgccgtgtc cttcgccgtg cccgccaaca ccgagggcct ctcgctctac
     2341 gtgtcgggct acggcggcgg gggaggcggc cacgacgcct tcgaccaccc cgtgtcctcc
     2401 cggcacaagc tgctcgagac cctcacggtc ttcgacgacg tgttcgtgcc gtgggagcgg
     2461 gtcttcctgt gccaggagcc ggagaaggcg ggggccctgg ccctgacctt cgtcgagtac
     2521 caccgtttca ccgcggtgtc ctacaagctc ccgctggtcg atgccttcgt gggggcggcg
     2581 gcccagatcg ccgagatgaa cggggtgatg aaggcgggcc acatccgcga caagctcacc
     2641 cacctggtgg cctacgccga gacggtccgg gccttgaccg aggccgccgg acttcggtcc
     2701 cggatcggcg agcacggcat cgcctacccg gaccccatga ccacgaacat ggccaagttc
     2761 accttcgcct cgggcttcta caaggccgtc gagtggctgc aggacatc
//
LOCUS       DASTFV010000050         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_168098, whole genome shotgun sequence.
ACCESSION   DASTFV010000050 DASTFV010000000
VERSION     DASTFV010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168098"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="VFP02_00900"
     CDS             complement(<1..1206)
                     /locus_tag="VFP02_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HET9541607.1"
                     /translation="MDHLAVAATAVAAAIGAVLASCEPTGWRPADIVWSAGFAVVVVL
                     AGAKARRWTWLTAAAIGAAAAPDLAVAAPALLGLVITLVSVVGERRTRVVGAVVLALA
                     LQSLLRLDLDDPYGLASLVAAAGVLPALVSGYRRASQPVRRTVQVGAVAFGLTATGLA
                     MAQAVVVVEARSSVAAGIDAAQSGFDAARDGEEAASVEQFDAAADAFAEANDSLTAPW
                     ALAGRAVPLLGQHARAVEDITSAGADLAETASGAAQDAPIEELRFTDGALDLRQVSEF
                     DAPLAEAEAALAAADDTVTTVNSGWLLPPLASRVDEFAAEIDEARPQAEIAQAGVAVA
                     PGLFGGHGLRRYFLAFVTPAEQRALGGFMGNFGVLTANEGDLNLARSDEIAALERPLR
                     AMDATVTAPP"
     gene            1466..>2286
                     /locus_tag="VFP02_00905"
     CDS             1466..>2286
                     /locus_tag="VFP02_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9541608.1"
                     /translation="MRVLVTGGAGFIGANLCRSLLADGHDVRVLDDLSTGRLGNLDGL
                     SLDLIEGSIVEPAVIAGAAADVDRIVHLAARASVPRSIAEPVAAHEANVTGTLNVLEA
                     ARPRGLPVIVASSSSVYGDTAVSPKHEDLPVAPRSPYAASKLATEGYALAYGVAYALP
                     VLALRFFNVYGPLQPADHAYAAVVPRFIDAALAGRPVEIHGDGRQSRDFTFVGTVVGV
                     LGRAVNEATTAPGPVNLAFGTRTDLLDLVARLEALLGQSIAVEHTDPRPGDVRHST"
BASE COUNT          291 a          854 c          856 g          285 t
ORIGIN      
        1 gggtggggcg gtgacggtgg cgtccatggc gcgcagtggg cgctcgagcg cggcgatctc
       61 gtcggaacgg gccaggttga ggtcgccctc gttggcggtg agcacgccga agttgcccat
      121 gaagcccccg agcgcccgtt gctcggcggg ggtgacgaag gccaggaagt agcggcgcag
      181 gccgtggcct ccgaacaggc ccggggccac ggcgacgcct gcctgggcga tctcggcctg
      241 ggggcgggct tcgtcgatct cggcggcgaa ctcgtcgacc cggctggcca gtgggggcag
      301 cagccagccg ctgttcaccg tcgtgaccgt gtcgtcggcg gccgccagag cggcctctgc
      361 ctcggccagg ggggcgtcga actccgacac ctgcctgagg tccagagcgc catcggtgaa
      421 ccggagctcc tcgatcgggg cgtcctgggc cgcgccggac gcggtctcgg ccaggtcggc
      481 gcccgccgag gtgatgtcct cgaccgctct ggcgtgctgg ccgaggagcg gcacggcccg
      541 tccggccagc gcccacgggg cggtcaggct gtcgttggct tcggcgaagg cgtcggccgc
      601 ggcgtcgaac tgctcgacgg aagcggcctc ctcgccgtcg cgggcggcat cgaacccgga
      661 ctgggcggcg tcgatgccgg ccgccacgga tgaacgggcc tcgaccacga ccacggcctg
      721 ggccatggcg aggccggtgg cggtgaggcc gaaggccacc gcacccactt gcacggtgcg
      781 tcggaccggt tggctggcgc gccggtaacc ggacacgagc gcggggagca ctccggccgc
      841 ggccaccaac gaggccaggc cgtagggatc gtcgaggtcc aggcgcagca ggctctggag
      901 ggcgagggcg aggaccacgg cccccacgac ccgggtccgt cgttccccca cgacgctgac
      961 cagcgtgatg accagaccga gcagggcggg ggcggccacg gccaggtcgg gggcggccgc
     1021 ggccccgatg gcggcggcgg tgagccaggt ccagcgccgg gccttcgcgc cggccaggac
     1081 gacgacgacg gcgaacccgg cggaccacac gatgtcggcc gggcgccagc ccgtgggctc
     1141 gcaggaagcg aggacggccc cgatggcggc agccacggcg gtggcggcga ccgcgaggtg
     1201 atccacccgg gtcgggggtc gggccggggg gtcagggggc aggaggggca tcgtggcagt
     1261 gcggagcgcc gaccccgagg acctcgggcc gaacgggccc gctgaatcta gcgaagcgag
     1321 ccgcgagaga gtcggcatgg ccggcgggct gccacactga ggcccccatg cgcttcctcg
     1381 ccaccggcgc gaccggcttc gtcggctccc ccctcgcccg gcacctggtc gacgacggcc
     1441 cggctgcctg ggccaccgct tagcggtgcg ggtcctggtc accggcgggg ccgggttcat
     1501 cggggccaat ctgtgccgct cgctgctcgc cgacggccac gacgtccgag tgctcgacga
     1561 cctgtcgacg ggccggctcg gcaacctcga cgggctgtcg ctcgacctga tcgaaggttc
     1621 gatcgtcgag cccgccgtca tcgcgggggc ggccgccgac gtcgaccgca tcgtccacct
     1681 ggcggcccgg gcctcggttc cccgctccat tgccgagccg gtcgccgccc acgaggcgaa
     1741 cgtcaccggc accttgaacg tcctcgaggc ggcccgaccc cgaggcctcc ccgtgatcgt
     1801 ggcgtcgtcc tcgtcggtgt acggcgacac ggccgtctcg ccgaagcacg aggacctccc
     1861 ggtcgcgcct cgcagtccct acgccgcctc caagctggcg accgagggct acgccctggc
     1921 ctacggcgtg gcctacgccc tccctgtcct ggccctgcgc ttcttcaacg tctacggccc
     1981 gctccagccc gccgaccacg cctacgccgc cgtcgtgccg cggttcatcg atgccgcgct
     2041 ggcgggccgg cccgtcgaga tccacggcga cggccgccag agccgggact tcaccttcgt
     2101 gggcaccgtg gtcggcgtcc tcggccgagc ggtgaacgag gccacgaccg cgcccgggcc
     2161 ggtcaacctg gccttcggca cccggaccga cctcctcgac ctcgtcgccc gcctggaagc
     2221 cctcctcggc cagtcgatcg ccgtggagca caccgacccc cgccccggcg atgtccgcca
     2281 ctcgac
//
LOCUS       DASTFV010000051         3154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_180470, whole genome shotgun sequence.
ACCESSION   DASTFV010000051 DASTFV010000000
VERSION     DASTFV010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180470"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..890
                     /locus_tag="VFP02_00910"
     CDS             18..890
                     /locus_tag="VFP02_00910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET9541609.1"
                     /translation="MRFSYAESMTDPANYLPLARAVEAAGYSSFIVPDSICYPEVSDS
                     TYPYTPDGNREFLDGAPFIEPFTLIPAMAAVTETLRFTTFVVKLPIRHPVLVAKQAGS
                     VAVMTNNRFGFGVGTSPWPEDYDICDVPWERRGKRMDEMIDIIRGLTTEGYFDYDGEI
                     FQLQSVKMSPTPTEPIPILIGGHGELALKRAAERGDGWMHAGGDPDELVRCLDRLKEL
                     RTMLGRENDPFEIHVISFDAWSVDGAHRLEDLGVTDAIVGFRDAYQPGPDTQPLQDKI
                     DALNQFADEVVAKV"
     gene            complement(900..1754)
                     /locus_tag="VFP02_00915"
     CDS             complement(900..1754)
                     /locus_tag="VFP02_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9541610.1"
                     /translation="MVAVLLGLAASAAFSCADFFGGMASRRANPWLVVLANQAIGLVP
                     LTVYLLLLTDHGPDGRDVALSMVAGVAAVAGLGLLFGGFAVGRMAVVAPVSAAVGGVV
                     PIAWGLANGERPGPVALLGAVVTLSAAVVLARSEGAATDTALEGGDDRRAALMAVGAG
                     LAFGVILICLSETREAAGLWPPMIAHFTAIPILTIGLVIARVSLVAPEPARRPMVASG
                     FFDATAVALLVLALRQDLVSLVAPAANLYPAGTVLLAALVLGERLRTVQVAALAAAMV
                     GLVLLSVG"
     gene            1815..2486
                     /locus_tag="VFP02_00920"
     CDS             1815..2486
                     /locus_tag="VFP02_00920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HET9541611.1"
                     /translation="MSVLDLWAAAQADMSALGRGLRADEADAPVPACPVWTVHDVFAH
                     QAGVASDILGGRLEGVTTDPWTQRQVEERAGRTLAEILDEWDADAPRLIEAMAPLGDA
                     VDPRMVIDVWTHDQDVRGAVGRPGQRNGERAEWVGDRFRDHLAEEVERAELAPLVVDF
                     GDRPAAEGSVAVSRFEFCRALVGRRSLDQIRQWTWTVDDPEPYVGLIPVFTARADALI
                     EPEAP"
     gene            2483..2716
                     /locus_tag="VFP02_00925"
     CDS             2483..2716
                     /locus_tag="VFP02_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HET9541612.1"
                     /translation="MSSTELLPDPVTTDTGEPEVAHIAPAADVNRAYVTGEAITALCG
                     VVFVPTRDPNRYPVCPPCKAVLDQIKAGRGGTN"
     gene            complement(2795..>3154)
                     /locus_tag="VFP02_00930"
     CDS             complement(2795..>3154)
                     /locus_tag="VFP02_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541613.1"
                     /translation="DRIGHGWRLVDDLDGPVAQEVRQRGLPLEVCLTSNACLGLPVEQ
                     HPVRRLFDAGFVVTVNPDDRAITTTTTSRELALWAEHHGFTDTELAAVQQAAVAAAFC
                     DDPTRAAVAAEVTAGWS"
BASE COUNT          488 a         1157 c         1133 g          376 t
ORIGIN      
        1 cggctagacc acagcccatg cggttctcct acgcggagtc gatgaccgac cccgccaact
       61 acctgcccct ggcccgagcg gtggaagcgg cgggctactc gtccttcatc gtccccgaca
      121 gcatctgcta tccggaggtg tcggactcga cgtaccccta cacgccggac gggaaccggg
      181 agttcctcga cggggcgccg ttcatcgagc cgttcacgct catcccggcg atggccgcgg
      241 tcaccgagac cctgcgcttc accaccttcg tcgtgaagct ccccatccgg cacccggtgc
      301 tggtggccaa gcaggccggc tcggtggcgg tgatgacgaa caaccgcttc ggcttcggcg
      361 tgggcaccag cccctggccc gaggactacg acatctgcga cgtcccctgg gagcgacggg
      421 ggaagcgcat ggacgagatg atcgacatca tccggggcct caccaccgag ggctacttcg
      481 actacgacgg cgagatcttc cagctccagt cggtgaagat gagcccgacc ccgaccgagc
      541 cgatcccgat cctcatcggc ggccacggcg agctcgccct gaagcgggcg gccgagcggg
      601 gcgacggctg gatgcacgcc gggggtgacc cggacgagct ggtgcgctgc ctggaccggc
      661 tgaaggagct gcggacgatg ctggggcggg agaacgaccc cttcgagatc cacgtcatca
      721 gcttcgacgc ctggagcgtc gacggggctc accgcctgga ggacctcggg gtcaccgacg
      781 ccatcgtcgg cttccgcgac gcctaccagc ccggccccga cacccagccc ctgcaggaca
      841 agatcgacgc actgaaccag ttcgccgacg aagtcgtcgc gaaggtctga cgtcggcctt
      901 cagcccacgc tgagcaggac cagtccgacc atcgccgcgg ccagcgccgc gacctgcacg
      961 gtccggaggc gctcgcccag cacgagggcg gccaggagca cggtccccgc cgggtagagg
     1021 ttggcggccg gggccaccag cgacacgagg tcctggcgca gggcgagcac gaggagggcc
     1081 accgccgtgg cgtcgaagaa gccgctggcc accatcggcc gtcgcgcggg ctcgggcgcc
     1141 acgagggaca cgcgggcgat gaccaggccg atggtcagga tcgggatggc cgtgaagtgg
     1201 gcgatcatcg gcggccagag gccggccgct tcgcgggtct cggacaggca gatgaggatg
     1261 acgccgaagg ccaggcccgc ccccaccgcc atcagcgcgg cccggcggtc gtcgcccccc
     1321 tccagcgccg tgtccgtggc cgccccctcg ctgcgggcca gcacgacggc ggccgacagc
     1381 gtgaccaccg cgccgagcag cgccaccggg ccagggcgtt cgccgttggc caggccccac
     1441 gcaatgggca ccaccccgcc gaccgccgcg ctgaccgggg ccaccacggc catccgtccc
     1501 accgcgaacc cgccgaacaa cagccccagc ccggccacgg cggccacccc cgcgaccatg
     1561 ctcagggcga cgtcccgacc gtccggtccg tggtcggtca ggagcaggag gtagacggtc
     1621 aggggcacga ggccgatggc ctggttggcg agcacgacga gccacggatt ggcccggcgc
     1681 gaggccatgc cgccgaagaa gtcggcgcac gagaacgcgg ccgatgccgc caggccgagc
     1741 aggaccgcca ccacggggcg agaccctagg gtgaggccga tgagcagcgg cacggacgtg
     1801 acgcctccgg cagcatgagc gttctcgacc tctgggcggc cgcccaagcg gacatgagtg
     1861 cgctggggcg gggcctgcgg gccgacgagg ccgacgcccc cgtgccggcc tgtccggtgt
     1921 ggacggtcca cgacgtgttc gcccatcagg ctggcgtggc cagcgacatc ctgggcggac
     1981 ggctggaggg cgtgaccacg gacccgtgga cccagcgcca ggtcgaggag cgggccgggc
     2041 gcacgttggc cgagatcctg gacgagtggg acgccgacgc cccccgcctc atcgaggcca
     2101 tggcccccct cggcgacgcc gtggatcccc gcatggtcat cgacgtgtgg acccacgacc
     2161 aggacgtgcg gggcgccgtc ggtcgccccg gccagcgcaa cggcgagcgg gccgaatggg
     2221 tgggcgaccg cttccgggat cacttggccg aggaggtcga gcgggccgag ctggcgccgc
     2281 tggtcgtcga cttcggtgac cggcccgcgg ccgaagggtc ggtcgccgtg agccgcttcg
     2341 agttctgtcg agccttggtc ggccgccgca gcctggacca gatccgccag tggacctgga
     2401 ccgtcgacga ccccgagccg tacgtcggcc tgatcccggt cttcaccgcc cgagccgatg
     2461 ccctgatcga gccggaggcg ccgtgagctc caccgagctg ctccccgatc ccgtcaccac
     2521 cgacaccggc gagcccgagg tggcccacat cgcccccgcg gccgacgtga accgggccta
     2581 cgtgaccggc gaggcgatca ccgcgctgtg tggggtggtc ttcgtcccca cccgggaccc
     2641 caaccgctac ccggtctgcc ctccctgcaa ggccgtcctc gaccagatca aagccggccg
     2701 cggcggcacc aactagcacc cgcccccggt gctggttgca cccagccgcg acccgccgcg
     2761 gggagaggca accagaacga acgtggacgg ggtgtcagga ccagccggcg gtgacttcgg
     2821 cggcgacggc ggcccgggtg gggtcgtcgc agaaggcggc ggcgaccgcg gcctgctgga
     2881 cggcggccag ctcggtgtcg gtgaagccgt ggtgctcggc ccagagggcc agctcccggg
     2941 acgtggtcgt ggtggtgatg gcccggtcgt cggggttgac cgtcaccacg aatccggcgt
     3001 cgaacaggcg gcgcaccggg tgctgctcca ccggcaggcc cagacaggcg ttggaggtca
     3061 ggcacacctc gaggggaagg ccccgctggc ggacctcctg ggccaccggc ccgtcgaggt
     3121 cgtcgaccag ccgccagccg tggccgatgc ggtc
//
LOCUS       DASTFV010000052         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_182884, whole genome shotgun sequence.
ACCESSION   DASTFV010000052 DASTFV010000000
VERSION     DASTFV010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182884"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VFP02_00935"
     CDS             complement(<1..1025)
                     /locus_tag="VFP02_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541614.1"
                     /translation="MADTLPGLGGPVDDESAESTDGAGLPDENRDDPEVQSGLGRGLA
                     AIIPTLAADLDTPPRTQRRKRRRGGLAALLGGPVEDEGAPAAESPAEPPPSSLPNAEP
                     VVAATEPSPGHEPAPSAVRKLRDDLVGALLDGLANTMALDLCAYLHEGPGEEPHLFLQ
                     SPSMDGIGAARTYALFDGLRVDLEAGGSEADFDAAGFRGVTVATVGAGSRGLWAVARV
                     EGITPPEREVAARFCRSFGRAVHQLDGAASPASAIATPVYVEAHPSRDSVLAEVQVRI
                     GDQVRPGHGRADTQVEAVTRAVLVATGSHVLFRYASEVDHEGEHAAVVLLEGGDQAVS
                     LGSALNPG"
     gene            1068..1370
                     /locus_tag="VFP02_00940"
     CDS             1068..1370
                     /locus_tag="VFP02_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HET9541615.1"
                     /translation="MANEPDDELVVVVPAAHQAGVWANDAIVNSSDHEVTVDLLRVDH
                     SVEPNVGTVVARVAMSPKLLRQLIDDLSVAWDAYVDEITDRLIDAPSDTGPVPPAD"
     gene            1421..2551
                     /locus_tag="VFP02_00945"
     CDS             1421..2551
                     /locus_tag="VFP02_00945"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET9541616.1"
                     /translation="MERPDLFARRNRLRILGLIILATVVYWMAVVAAAVATGLIVIFY
                     IISEAGVPDDFEVLKWFGYFCLAVIALSIVIGSLMSIVRLPTLRRKLEAQVLRETGAQ
                     LANPDAYPQVRNLLDGLALSAGVPAPRFAVIQDAVPNSFGVGTRPNKTIIGITSGLVD
                     ELNRDELEAALSYEISRIGSWDIALSSWAVALTSGAIANVEADDLRSIVGWIPGRMAE
                     WLQAWALRDQGEDRDRIAVQFTRHPEALITALEKLEADQGEIRRVSRATAPLWLEVPD
                     GVYASALSSRSKRLGTSLLLQQRIGRLTELAGLPPRPKVVPKARPLPPPPQAPAPAAP
                     APPGSPVAGVPVVGRAVMPHLPPPPTVPPPPGGGGGGGFRVS"
BASE COUNT          341 a          950 c          937 g          340 t
ORIGIN      
        1 cccgggttga gggcggagcc gagggacacg gcctggtccc cgccctcgag gaggacgacg
       61 gcggcgtgct caccctcgtg gtcgacctcg ctggcgtagc ggaacaggac gtggctcccg
      121 gtggcgacca gcaccgcccg ggtgacggcc tccacctggg tgtcggcccg gccgtggccg
      181 ggacggacct gatccccgat gcggacctgg acctcggcca gcaccgagtc ccgggacggg
      241 tgggcctcga cgtagacggg cgtggcgatg gccgaggccg gcgacgcggc gccgtcgagc
      301 tggtggacgg cgcgaccgaa cgaccgacag aaccgagccg ccacctcccg ctccggcggg
      361 gtgatcccct cgacccgggc cacggcccag agcccgcggg agccggcccc gacggtggcc
      421 acggtgacgc cccggaagcc ggccgcgtca aagtctgcct ccgacccgcc tgcttcgaga
      481 tcgacgcgca ggccgtcgaa gagcgcgtac gtgcgggccg ccccgatgcc gtccatcgac
      541 ggagactgca ggaacaggtg aggctcctcg cccgggccct cgtggaggta ggcgcacagg
      601 tcgagggcca tcgtgttggc caggccgtcg agcagggccc ccacgaggtc gtcgcggagc
      661 ttgcggacgg ccgacggcgc gggctcatgg cccggggacg gttcggtggc ggcgaccacg
      721 ggctcggcgt tcggcaacga cgacggtggt ggctcggcgg gcgactccgc agccggtgcc
      781 ccctcgtcct cgaccggtcc gccgagcagg gcggccaacc caccgcggcg ccgcttgcgg
      841 cgctgggtcc ggggcggggt gtcgaggtcg gccgccaggg tcgggatgat ggcggccagc
      901 cctcggccga gcccgctctg gacctcgggg tcgtcgcgat tctcgtcggg gagaccggct
      961 ccatcggtcg attcggcgga ctcgtcgtcc accggaccac cgaggccggg cagggtgtcg
     1021 gccacccgcc tacggtacgg accggggcgg accgagtggg ggacccgatg gcgaacgaac
     1081 ccgatgacga gctggtggtg gtggtgcccg ccgcccacca ggcaggggtg tgggccaacg
     1141 acgccatcgt gaactccagt gatcacgagg tcaccgtcga cctgctgagg gtcgatcact
     1201 ccgtcgagcc caatgtgggc accgtcgtcg cccgcgtcgc catgtcgccc aagctgctgc
     1261 gtcagctcat cgacgacctg agcgtcgcct gggacgccta cgtcgacgag atcaccgacc
     1321 ggctgatcga cgctccttcg gacaccggtc cggtgccacc cgcggattga gtagcgcgta
     1381 cccacgtacg ggcccggacg gacgcgtagc gtccccaccc atggagcggc ccgatctctt
     1441 cgcccggcgc aaccgactgc gcatcctcgg cctgatcatc ctggccacgg tcgtgtactg
     1501 gatggccgtc gtagccgccg cggtggccac cgggctgatc gtcatcttct acatcatcag
     1561 cgaggcgggg gtcccggacg acttcgaggt gctcaagtgg ttcggctact tctgcctggc
     1621 cgtcatcgcc ctctcgatcg tgatcggcag cctgatgtcg atcgtccgcc tgcccaccct
     1681 gcgtcggaag ctcgaggccc aggtgctgcg ggagaccggg gcccagctgg cgaaccccga
     1741 cgcctacccc caggtccgca acctcctgga cggcctggcc ctgtccgccg gcgtcccggc
     1801 cccccgcttc gccgtcatcc aggacgcggt gccgaactcc ttcggggtcg gcacccggcc
     1861 caacaagacg atcatcggca tcacctccgg cctcgtcgac gagctgaacc gggacgagct
     1921 ggaggcggcg ctgtcctacg agatcagccg gatcgggagc tgggacatcg ccctgtcgtc
     1981 ctgggccgtg gccctgacct cgggggccat cgccaacgtc gaggccgacg acctccggtc
     2041 catcgtcggg tggatcccgg gccggatggc cgaatggctc caggcgtggg cgctgcggga
     2101 ccagggcgag gaccgggacc gcatcgccgt ccagttcacc cggcaccccg aggccctgat
     2161 caccgcgctg gagaagctgg aggccgacca gggcgagatc cgccgggtga gccgggccac
     2221 ggccccgctg tggctggagg tgcccgacgg ggtctacgcc agcgccctct cgtcccggtc
     2281 caagcgcttg ggcacgtcgc tcctcctcca gcagcgcatc ggccggctga ccgagctggc
     2341 cgggctaccg ccgcggccga aggtggtgcc gaaggcgcga cccctgccgc ccccgccgca
     2401 ggcgccggcg ccggccgcgc cagcgccgcc gggctcgccc gtcgcgggtg tcccggtcgt
     2461 cggccgggcg gtgatgccac acctcccgcc gccgccgact gtacccccgc ccccgggcgg
     2521 gggtggcggc ggcgggttcc gcgtcagctg aggcggccga gcgcacag
//
LOCUS       DASTFV010000053         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_183003, whole genome shotgun sequence.
ACCESSION   DASTFV010000053 DASTFV010000000
VERSION     DASTFV010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183003"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFP02_00950"
     CDS             <1..556
                     /locus_tag="VFP02_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET9541617.1"
                     /translation="LLKENIIFLGTPIDDTIANLVCAQLLHLESENPDKDINIYINSP
                     GGDITALFAIYDTMQYIKPDIATICYGQAASAAAVLLAAGAPGKRLALPHSRILLHQP
                     YAGAQGQATDIELAAKEILRLRELLEEILAEHTGQPIERLHNDTDRDFVMTAAEAREY
                     GIIDEVIDTRSMAEAGAPIAAVSS"
     gene            578..1852
                     /gene="clpX"
                     /locus_tag="VFP02_00955"
     CDS             578..1852
                     /gene="clpX"
                     /locus_tag="VFP02_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET9541618.1"
                     /translation="MAKFGDGGELLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEELAETSDVSFDELPKPREIFEFLNDYIVGQSHAKKILSVAVYNHYKRMQMGGAD
                     SEVELAKSNILLIGPTGCGKTLLAQTLARMLNVPFAIADATALTEAGYVGEDVENILL
                     KLIQAADYDVKKAETGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTTAS
                     VPPQGGRKHPHQEFIQIDTTNILFICGGAFAGLEKIIESRVGRKGVGFGADIRGIDEK
                     DLGSIYGNVLPEDFLKFGLIPEFVGRLPVVGAVSNLDAEALIEILVEPKNALTRQYSR
                     LFEFEDVELEFKDDALEAIAELALLRGTGARGLRAILEEVLNEVMFELPSRGDVSKCV
                     IDRQVVEESAVPTLVPRTDPSTRPARPRRAAS"
     gene            1849..2274
                     /locus_tag="VFP02_00960"
     CDS             1849..2274
                     /locus_tag="VFP02_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541619.1"
                     /translation="MNRAARGALIGGAVGALVAVAQTVRAEDDPPQVVAVHAVRGAAQ
                     GALVGALVGWVLDRTSAKPASGVAAYTEVAKDAWTGAVDTAEQAYEVARPRLEHAYEA
                     TRPRVEHAVEVALPVVELATELARERAQDLAEAARRRAA"
     gene            2271..>2920
                     /locus_tag="VFP02_00965"
     CDS             2271..>2920
                     /locus_tag="VFP02_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET9541620.1"
                     /translation="MNPREARSWLDGHLNLEAGAGAVTAGVVDALSLDRMRLLVGVLG
                     DPQTAYPVIHLTGTNGKGSTARMLEAILRGHGLSVGLYTSPHLEALNERLVWDGRPIP
                     DEELAEVFDELATLEPLVDGELTWFELVTAAAFAWFAQQAVDVAVVEVGLLGRYDATN
                     VADGLVAVVTNVGQDHTDGRDRWREAIAGEKAGIIKPGSTLVLGETDWELQKIFEA"
BASE COUNT          498 a         1029 c          977 g          416 t
ORIGIN      
        1 cctgctcaag gagaacatca tcttcctggg cacgccgatc gacgacacga tcgccaacct
       61 ggtctgcgcc cagctcctgc acctcgagtc cgagaacccg gacaaggaca tcaacatcta
      121 catcaacagc ccgggcggtg acatcacggc gctgttcgcc atctacgaca cgatgcagta
      181 catcaagccc gacatcgcca ccatctgcta cggccaggcc gcctcggccg ccgcggtcct
      241 gctcgccgcg ggggcgccgg gcaagcgcct cgccctcccg cactcccgga tcctgctcca
      301 ccagccctac gccggggccc agggccaggc caccgacatc gagctggcgg ccaaggagat
      361 cctgcgcctg cgggagctcc tggaggagat cctggccgaa cacaccggtc agccgatcga
      421 gcgtctgcac aacgacaccg accgggattt cgttatgacc gccgcggagg cccgcgaata
      481 cggcatcatc gacgaggtga tcgacacccg ctcgatggcc gaggccggcg caccgatcgc
      541 agccgtcagt tcctgacacc gacaggggga tctcgaggtg gccaagttcg gagacggggg
      601 ggagctgctc aagtgctcct tctgtggcaa gtcccagaag caggtcaaga agctcatcgc
      661 cggtcccggc gtctacatct gcgacgagtg catcgacctc tgtaacgaga tcatcgagga
      721 ggagctggct gagacgtccg acgtcagctt cgacgagctc cccaagcccc gggagatctt
      781 cgagttcctg aacgactaca tcgtcggtca gagccacgcc aagaagatcc tgtccgtcgc
      841 cgtctacaac cactacaagc ggatgcagat ggggggcgcg gacagcgagg tcgagctggc
      901 caagtccaac atcctcctca tcgggcccac cggctgcggc aagacgctgc tggcccagac
      961 cctggcccgg atgctgaacg tgcccttcgc catcgccgac gccacggcgc tgacggaggc
     1021 gggctacgtc ggcgaggacg tcgagaacat cctgctcaag ctgatccagg ccgccgacta
     1081 cgacgtcaag aaggccgaga cggggatcat ctacatcgac gagatcgaca agatcgcccg
     1141 caagagcgag aacccgtcga tcacccgtga cgtgtcgggc gaaggcgtgc agcaggccct
     1201 gctcaagatc ctggagggga ccaccgcctc ggtcccaccc caggggggcc gcaagcaccc
     1261 ccaccaggag ttcatccaga tcgacaccac gaacatcctg ttcatctgtg gcggggcctt
     1321 cgccggcctc gagaagatca tcgagtcccg ggtcgggcgc aagggcgtgg gcttcggcgc
     1381 cgacatccgg ggcatcgacg agaaggacct cggctccatc tacggcaacg tcctgcccga
     1441 ggacttcctg aagttcgggc tgatccccga gttcgtcgga cgcctgccgg tggtcggcgc
     1501 ggtctccaac ctggacgccg aggcgctcat cgagatcctg gtcgagccca agaacgccct
     1561 gacccgccag tacagccggc tgttcgagtt cgaggacgtc gagctggagt tcaaggacga
     1621 cgcgctggag gccatcgccg agctggccct gctccggggc accggcgccc gaggcctgcg
     1681 ggccatcctc gaagaggtgc tcaacgaggt catgttcgag ctgccgagcc gcggtgacgt
     1741 ctccaagtgc gtcatcgacc gccaggtcgt ggaggagagc gcggtcccga ccctggtgcc
     1801 ccgcaccgac ccgtcgaccc gtcccgctcg gccccgccgc gccgcctcgt gaaccgggcg
     1861 gcccggggcg ctctgatcgg gggcgcggtc ggcgcgctgg tcgccgtcgc ccagaccgtg
     1921 cgagccgagg acgatccgcc gcaggtggtc gccgtgcacg ccgtacgcgg tgcggcccag
     1981 ggggccctgg tcggggccct cgtcggctgg gtgctggacc gcacctcggc caagccggcc
     2041 tccggagtcg cggcctacac cgaggtggcc aaggacgcgt ggaccggtgc ggtggatacg
     2101 gccgagcagg cctacgaggt ggcccgcccc cggttggagc atgcctacga ggcgacccgc
     2161 ccccgggtcg agcacgccgt cgaggtcgcg cttccggttg tcgagctggc caccgagctg
     2221 gctcgcgaac gggcccagga cctcgctgag gcagcccgcc gccgcgccgc gtgaacccgc
     2281 gcgaagcgcg gtcgtggctg gacggccacc tcaacctgga agcgggcgcc ggtgcggtga
     2341 ccgcaggcgt ggtcgacgcc ctctcgctgg accggatgcg gttgttggtc ggggtgctgg
     2401 gtgaccccca gaccgcctat ccggtcatcc acctgaccgg gaccaatggc aagggctcca
     2461 cggctcggat gctcgaagcg atcctgcggg ggcacggcct ctccgtcggg ctctacacca
     2521 gcccccatct cgaagcgctc aacgagcggc tggtgtggga cggccgaccc atccccgacg
     2581 aggagctggc cgaggtcttc gacgagttgg ccacgctcga gccgctggtc gacggcgagc
     2641 tgacctggtt cgagctggtg accgcggctg ccttcgcctg gttcgcccag caggccgtcg
     2701 atgtcgcggt cgtcgaggtc ggcctcctcg gccgctacga cgccaccaac gtggccgacg
     2761 gcctcgtcgc cgtggtcacc aacgtcggtc aggaccacac cgacggccgc gaccgttggc
     2821 gagaggcgat cgcgggggag aaggccggga tcatcaagcc cggctccaca ctcgtgctgg
     2881 gcgagactga ctgggagctc cagaagatct tcgaggccga
//
LOCUS       DASTFV010000054         3523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_184133, whole genome shotgun sequence.
ACCESSION   DASTFV010000054 DASTFV010000000
VERSION     DASTFV010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184133"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VFP02_00970"
     CDS             <1..850
                     /locus_tag="VFP02_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9541621.1"
                     /translation="EGRTVEQGIELFGPPTRQVTVAASPLGPSGARLGAVVLVRDESR
                     LRRLEAIRQEFVANVSHELKTPIGAIALLAETLAATDDPADAARRIDLLVEEAHRLGR
                     IIDDLLELGEVEGRDRSHEPVDLADLVEESVRGLRPVAEQLGIELDLELDRGQTCLAD
                     PRQLGSAITNLIDNALKYADGRSPVSILVQPGDGWADVVVQDHGIGIPQPEQERVFER
                     FYRVDKARSRDTGGTGLGLAIVRNVARAHGGEVLVTSREGEGSTFTLRLPLVSVPAGD
                     KEARRG"
     gene            843..1583
                     /locus_tag="VFP02_00975"
     CDS             843..1583
                     /locus_tag="VFP02_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9541622.1"
                     /translation="MADAPTVLVVEDEENFIEALTIGLKKEGFRVQIARDGAEALLVF
                     DAVRPDIVLLDLMLPKVSGVDVCREMRSRSKVPIIMVTAKGSEVDTVVGLEVGADDYV
                     TKPYRLRELVARMRAVLRRAAERTPDRTPGNGDEVLASGDLVLDPDRHEVSLHGDALD
                     LPLKEFEVLQLLLERAGRVVTRDQLIDEVWGHDYVGDTKTLDVHIKRLRSRIEADPSV
                     PERILTVRGVGYKIVAPDRADPRVASIP"
     gene            1646..3211
                     /locus_tag="VFP02_00980"
     CDS             1646..3211
                     /locus_tag="VFP02_00980"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9541623.1"
                     /translation="MSRRVGWNVFEGPGGPPERSADHFVASPFTRLARVHALQVAGDA
                     LIALALANSLFFSIPSGEARGQVALYLVLTMAPFAVIAPFIGPAIDRAANGRRLMVIL
                     IAFSRVLIGLFMIGNLDDLLLFPAALLMLVSGKAYGIAKSAIVPTTVSTDQELIEANS
                     KLSIITGFSGLAAGIPGVILGWLGGPTWIIGFAIVVFAVGTVLAFSLPQAAVAAEPVG
                     EAEEAELRSRGIFLAASSMGLMRAIVGFLVFLLAFAMREQPTWHLGFAVGASVLGSFV
                     GSILAPRVRRTTQEEDILVGSMLLATVAALLAAWSGGLGAAVLVAFAVGFSANTAKLA
                     FDSIVQRDAPDANRGRSFAKFETRFQIFWVVGAFIPVVLHLPARLGFLTIAVAAGFAG
                     ITYWIGRRDAAAGRPPRTSRGSRRPPRPPREERDRTDVSPPPDPTVVDDVLAIPHPGV
                     VEDFLPPGPTVVGDVPDASEPDGAEDPLAPGPDRRPRNPGRRPNTTGPTRKPPGASRR
                     RRPTQRNPRRRDL"
     gene            complement(3298..>3523)
                     /locus_tag="VFP02_00985"
     CDS             complement(3298..>3523)
                     /locus_tag="VFP02_00985"
                     /inference="COORDINATES: protein motif:HMM:NF021608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HET9541624.1"
                     /translation="VTTDESSRFVERLLGLELTQDQCDRGETFVAGVVERAGKRGLER
                     LWRSERELPTPAEVDAPGLWLARIDIPDPD"
BASE COUNT          467 a         1314 c         1202 g          540 t
ORIGIN      
        1 cgaggggagg accgtcgaac agggcatcga gctgttcggg ccccccaccc gccaggtcac
       61 ggtggccgcg tcaccgcttg ggcccagcgg cgcacggctg ggcgcggtcg tgctggtgcg
      121 ggacgagtca cggctgcgcc ggctcgaggc gatccgccag gagttcgtgg cgaatgtgag
      181 ccatgagctc aagaccccga tcggagcgat cgccctcctg gccgagacct tggccgccac
      241 cgacgacccc gccgacgcgg cgcgccgcat cgacctgctc gtcgaggagg cccaccggct
      301 cggccggatc atcgacgacc tcctcgagct gggtgaggtc gagggccgcg accgcagcca
      361 cgagcccgtc gacctcgccg acctcgtcga ggagtcggtc cggggcctgc gcccggtggc
      421 cgaacagctg gggatcgagc tcgacctcga gctggaccgg gggcagacct gcctggccga
      481 cccccgtcag ctcggatccg cgatcaccaa cctgatcgac aatgctctca agtacgccga
      541 tggccgctcg ccggtctcga tcctcgtcca gccgggtgat ggctgggccg acgtcgtggt
      601 gcaggaccac ggcatcggca taccgcagcc ggagcaggaa cgggtcttcg agcgcttcta
      661 ccgggtggac aaagcccgca gccgggacac cggtggcacc ggcctcgggt tggccatcgt
      721 ccgcaacgtg gcccgggccc acggcggcga ggtcctggtc acctcccgtg aaggggaggg
      781 ctccaccttc accctccgcc tgcccctcgt gtccgtaccc gctggtgaca aggaggctcg
      841 tcgtggctga tgccccgacc gtgctcgtgg tcgaggacga ggagaacttc atcgaggcct
      901 tgaccatcgg gctcaagaag gagggattcc gggtccagat cgcccgcgac ggggccgagg
      961 ccctgctggt gttcgacgcc gtccgtcccg acatcgtcct gctcgacttg atgctgccga
     1021 aggtctccgg cgtggacgtc tgccgggaga tgcggtcgcg ctccaaggtc ccgatcatca
     1081 tggtcacggc caagggctcc gaggtcgaca ccgtcgtggg cctcgaggtc ggcgccgacg
     1141 actacgtgac caagccgtac cgactgcgcg agctggtggc ccggatgcgg gccgtgctgc
     1201 gacgggcagc cgaacggacc cccgaccgga caccgggcaa cggcgacgag gtcctggcct
     1261 cgggcgacct ggtcctcgat cccgaccgcc acgaggtctc gctccacggc gatgcgctgg
     1321 acctgcccct caaggagttc gaggtcctgc agctgctgct cgagcgggcg ggccgggtgg
     1381 tcacccggga tcagctcatc gacgaggtct ggggccacga ctacgtgggc gacaccaaga
     1441 ccctcgacgt gcacatcaag cgcctccgca gccggatcga ggccgacccg tcggttcccg
     1501 agcgcatcct cacggtgcga ggtgtggggt acaagatcgt ggctccggac cgagccgatc
     1561 cccgagtagc gtcgattccg tagccttccg actccgcgag cggagtgagg atgccgttgc
     1621 cgtcgaccgc cgtacagtcg cctccgtgag ccgtcgcgtg gggtggaacg tgttcgaggg
     1681 cccgggcggg ccgcccgagc gctcggccga ccacttcgtc gcctccccgt tcacccgtct
     1741 ggcccgggtc catgccctcc aggtggcggg tgacgccctg atcgcgctgg ccctggccaa
     1801 ctcgctcttc ttctccatcc ccagcggcga ggcccggggc caggtcgccc tctacctggt
     1861 gctgaccatg gcgccgttcg ccgtgatcgc ccccttcatc ggtccggcca tcgaccgggc
     1921 ggccaacggc cgacggctga tggtcatcct gatcgccttc tcccgggtcc tcatcggcct
     1981 gttcatgatc gggaacctgg acgacctgct cctcttcccg gcggccttgc tcatgctcgt
     2041 gtccgggaag gcctacggga tcgccaagag cgccatcgtc ccgaccacgg tgtccaccga
     2101 ccaggagctg atcgaggcca actccaagct gtcgatcatc accggcttct cgggcctggc
     2161 cgcggggatc ccaggcgtga tcctgggctg gctcgggggg ccgacctgga tcatcggctt
     2221 cgccatcgtg gtgttcgccg tcggcacggt cctggccttt tcgctcccac aggctgccgt
     2281 ggccgcggag ccggtggggg aggccgagga ggccgagctg cgcagtcgcg gcatcttcct
     2341 ggccgcctcc tcgatgggcc tcatgcgggc catcgtcggc ttcctcgtct tcctgctggc
     2401 cttcgccatg cgggaacagc cgacgtggca cctcggcttc gccgtggggg ccagcgtcct
     2461 gggctccttc gtcggctcga tcctggcccc gcgggtccgg cgcacgaccc aggaggagga
     2521 catcctcgtg gggtcgatgc tcctggccac ggtggcggca ctgctggcgg cctggtccgg
     2581 cggcctcggg gccgcggtgc tggtcgcctt cgccgtcggc ttcagcgcca acacggccaa
     2641 gctggcgttc gactcgatcg tccagcgcga cgcccccgac gccaaccggg ggcggtcgtt
     2701 cgccaagttc gagacccgct tccagatctt ctgggtggtc ggcgcgttca tccccgtggt
     2761 cctccacctc ccggcccgct tgggcttcct gaccatcgcc gtggcggccg gcttcgccgg
     2821 catcacctac tggatcggcc ggcgggacgc ggcggccggc cgcccgcccc ggaccagccg
     2881 tggctcgcgc cgcccgcccc gcccaccccg ggaagagcgg gaccgaaccg acgtcagccc
     2941 gccgcccgac cccacggtcg tggacgatgt cctggccatt ccccatccgg gggtcgtcga
     3001 ggacttcctc cctcccggtc ccaccgtggt cggcgacgtc ccggacgcct ccgaaccgga
     3061 tggcgcggag gaccccctgg cgcccggccc cgatcgccgc ccccgcaacc ccgggcgtcg
     3121 cccgaacacc accgggccga cccgcaagcc acccggtgcg tcccgccgcc gccgccccac
     3181 ccagcgcaac ccccgccgtc gcgacctctg accgacaacg aggcaacaag cggtgactac
     3241 tttccgttct ggcgcctgat tgcggcgcga tagcccctca atcgggcgcc agaacggtca
     3301 gtcggggtcg gggatgtcga tgcgggccag ccagaggccg ggggcgtcga cttcggcggg
     3361 ggtgggcagc tcccgctcgc tgcgccagag gcgctccagg ccccgcttgc ccgcccgctc
     3421 gaccacgccg gcgacaaagg tctcgccccg gtcgcactgg tcctgggtca gctccaggcc
     3481 gagcaggcgc tcgacgaagc ggctggattc gtcggtggtg acc
//
LOCUS       DASTFV010000055         3380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_184746, whole genome shotgun sequence.
ACCESSION   DASTFV010000055 DASTFV010000000
VERSION     DASTFV010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184746"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VFP02_00990"
     CDS             <1..722
                     /locus_tag="VFP02_00990"
                     /inference="COORDINATES: protein motif:HMM:NF037564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HET9541625.1"
                     /translation="RDTDIDLLGALGRPVFVWGGANEGVAARVATANVVSFNVDPDAA
                     EDKFRDDTRAAPDNLFIDSTEAFWDKAGDASPARAPLPVPQAAPVDGAPLPGTPTPGV
                     AVDYGGTQAAFVWDVDRDGWVRLQDGTPHVVADGTVLAPTNVVLLVTPYGTAPSDQNS
                     PIALTTGTGDATVLIGGNAVDATWSRPEATDPYTLTDDATGEPLVLAPGRTWIGLPDS
                     GVTRLDQAQAAELASLVPAAG"
     gene            746..1639
                     /gene="pdxS"
                     /locus_tag="VFP02_00995"
     CDS             746..1639
                     /gene="pdxS"
                     /locus_tag="VFP02_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET9541626.1"
                     /translation="MADSTDRQTGTFRVKRGLAEMLTGGVIMDVVDPDQAKVAEDAGA
                     TAVMALERVPSDIRRDGGVARMSDPEMIEGIKAVVTIPVMAKARIGHFTEAQILQSLG
                     VDYVDESEVLTPADEAHHIDKWAFDVPFVCGATNLGEALRRISEGAAMIRSKGEAGTG
                     NIVEAVRHLRSILGDIRKVTQADSAELFDWAKQLQSPLPLVQEIAETGHLPVPMFCAG
                     GIATPADAALVMQLGAEAVFVGSGIFKSSNPPLVAKAIVEATTNFRDADILAKVSRGL
                     GDAMPGLEIDALETRYGDRGW"
     gene            1652..2242
                     /gene="pdxT"
                     /locus_tag="VFP02_01000"
     CDS             1652..2242
                     /gene="pdxT"
                     /locus_tag="VFP02_01000"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET9541627.1"
                     /translation="MRIGVLALQGASAAHVDVLRRLGAEAVTVKTPSDLDGVDGVVLP
                     GGESTTISFLLDSNELRRPLAERLAEGMPAFGTCAGMILLASEVLDGRADQRSFGAID
                     LAVRRNAFGRQLDSFEADLDVSGLDGPFHAVFIRAPGVESTGAGVEVLAEVDGRPVLC
                     RDGAIMVASFHPELTDDLRLHEMFLESFDRREVTQA"
     gene            2239..2991
                     /locus_tag="VFP02_01005"
     CDS             2239..2991
                     /locus_tag="VFP02_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9541628.1"
                     /translation="MSGHSKWATIKHKKGAADIKRGKLFGKLIRQVEVAAREGGGDPE
                     MNPTLRTMYQKARDSSVPLDTIERAIKRGTGELEGVTYEQVTYEGYAPNGVAVLVEVL
                     TDNRNRAGSEIRSLFTRGGGSLAEPGAVAWQFERKGVIVVPKSAEEDDVMMVGLDAGL
                     EDLTDEGDTWRLTSAATDVPDLRTALEDAGIAVESSDVTMLAKAEVPLDSAEKAKAVL
                     RLIDELDDHDDVQDVYANFDIPDDVLQAAAAD"
     gene            3118..3288
                     /locus_tag="VFP02_01010"
     CDS             3118..3288
                     /locus_tag="VFP02_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541629.1"
                     /translation="MTPATITDEEIETELLATSVTVAHDDDGDGTDGDDGGADDGDGD
                     TGGGDDDGGADA"
BASE COUNT          531 a         1205 c         1187 g          457 t
ORIGIN      
        1 cccgcgacac cgacatcgac ctgctgggcg cgctcggacg gccggtgttc gtctggggcg
       61 gggccaatga aggggtggcg gcccgggtgg ccaccgcgaa cgtggtcagc ttcaacgtcg
      121 atcccgacgc cgcggaggac aagttccgcg acgacacccg cgccgccccc gacaacctgt
      181 tcatcgactc gacggaggcc ttctgggaca aggcgggcga cgcctcgccg gcgcgggccc
      241 cgctgccggt gccccaggct gctccggtcg acggggcccc gctcccgggc acgccgactc
      301 ccggggtggc ggtcgactac ggcgggacgc aggccgcgtt cgtgtgggac gtggaccggg
      361 acggatgggt ccgcctccag gacgggaccc cgcacgtcgt cgccgacggc acggtcctgg
      421 ccccgaccaa cgtcgtgctg ctcgtcacgc cctacggcac cgccccgtcg gaccagaact
      481 cgccgatcgc cctgaccacg ggcaccggtg acgccaccgt gctgatcggc ggcaacgccg
      541 tcgacgccac ctggagccga cccgaggcga ccgacccgta caccctgacc gacgacgcaa
      601 ccggcgagcc cctggtgctg gcgccgggcc gtacctggat cggcttgccg gactccggcg
      661 tgacccgact ggaccaggcc caggcggccg aactggcctc cctggtcccc gcggccggct
      721 gatccggctc tcgtacactc cgggcatggc cgactccacc gatcgtcaga cgggcacgtt
      781 ccgcgtcaag cggggcctgg ccgagatgct gaccggcggc gtgatcatgg acgtcgtcga
      841 tcccgaccag gcgaaggtcg ccgaagacgc cggcgccacc gccgtcatgg ctctggagcg
      901 ggtcccctcc gacatccgcc gcgacggtgg cgtggcccgc atgagcgacc cggagatgat
      961 cgagggcatc aaagccgtcg tgaccatccc ggtcatggcc aaggctcgca tcggccactt
     1021 caccgaggcc cagatcctgc agtccctcgg tgtggactac gtcgacgaga gcgaggtcct
     1081 gaccccggcc gacgaggccc accacatcga caagtgggcc ttcgacgtcc cgttcgtctg
     1141 cggcgcgacc aacctgggcg aggccctgcg ccgcatctcg gagggggcgg ccatgatccg
     1201 ctcgaagggc gaagccggca ccggcaacat cgtcgaggcc gtgcgccacc tgcgctcgat
     1261 cctgggtgac atccgcaagg tgacccaggc cgactcggcc gagctcttcg actgggccaa
     1321 gcagctgcag tcacccctcc cgctggtgca ggagatcgcc gagaccggcc acctgccggt
     1381 accgatgttc tgcgccgggg gcatcgccac gccggccgac gccgctctgg tcatgcaact
     1441 cggggccgag gccgtcttcg tcggttctgg catcttcaag agctccaacc cgccgctggt
     1501 ggccaaggcc atcgtcgagg ccacgaccaa cttccgggac gccgacatcc tggccaaggt
     1561 cagccgcggc ctgggcgacg ccatgcccgg cctggagatc gacgccctcg agacccggta
     1621 cggcgaccgg ggctggtgat ggagcctccg ggtgcgcatc ggcgtactgg ctctccaggg
     1681 ggcgtcggcc gcccacgttg acgttctgcg ccggctgggc gcggaggcgg tgacggtcaa
     1741 gaccccttcc gatctggacg gcgtcgacgg tgtcgtcctc cccggcggcg aatcgaccac
     1801 gatctcgttc ctgctggaca gcaacgagct gcggcggccg ctggccgagc gcctggcgga
     1861 gggcatgccc gccttcggca cctgcgccgg gatgatcctg ctggccagcg aggtgctgga
     1921 cggccgggcg gaccagcggt ccttcggcgc gatcgacctg gccgtgcgcc gcaacgcctt
     1981 cggccgccag ctcgactcct tcgaggccga cctggacgtg tccggcctgg atgggccgtt
     2041 ccacgccgtg ttcatccggg cgccgggcgt ggagtcgacg ggggccggcg tcgaggtcct
     2101 ggccgaggtg gacggccggc ccgtgctctg tcgcgacggc gcgatcatgg tggcgtcgtt
     2161 ccaccccgag ctgaccgacg acctgcgcct gcacgagatg ttcctggaat cgttcgaccg
     2221 acgagaggtg acccaagcat gagcggccat tccaaatggg cgaccatcaa gcacaagaaa
     2281 ggggccgccg acatcaagcg gggcaagctc ttcggcaagc tgatccgcca ggtcgaggtg
     2341 gcggcccgcg agggtggcgg cgacccggag atgaacccga ccctgcgcac catgtaccag
     2401 aaggccaggg acagctcggt gcccctggac accatcgagc gggccatcaa gcgggggacc
     2461 ggcgagctgg agggcgtcac ctacgagcag gtgacctacg agggctatgc ccccaacggg
     2521 gtggccgtgc tcgtcgaggt gctgaccgac aaccggaacc gggccggctc ggagatccga
     2581 tcgctcttca cccgcggcgg gggctcgctg gccgagccgg gagcggtggc atggcagttc
     2641 gagcgcaagg gcgtgatcgt cgtccccaag tccgccgagg aggacgacgt gatgatggtc
     2701 ggcctcgatg ccggcctcga ggacctgacc gacgagggcg acacctggcg cctgacctcg
     2761 gccgcgaccg atgtacccga cctgcggacc gcgttggagg acgccggcat cgcggtggag
     2821 tcctccgacg tgaccatgct ggcgaaggcc gaggtgcccc tggactcggc cgagaaggcc
     2881 aaggccgtcc tccgcctcat cgacgagctg gatgatcacg acgacgtcca ggacgtctac
     2941 gccaacttcg acatacccga cgacgtgctc caggccgccg ccgcggactg agatcggacc
     3001 tgcgttcgcc aggccacgct gcggagtcgg cagcgaagca gctctgccga cgttcgtcga
     3061 aagctgccgt aggcagcagg agccgtgccg acggcgacga gggatattgt tcggcccatg
     3121 accccagcaa cgatcaccga cgaagagatt gagaccgagc tcctggccac ctccgtgacc
     3181 gttgctcacg acgacgacgg cgacggcacc gatggtgacg acggcggcgc ggacgacggt
     3241 gacggcgaca ccggtggagg cgacgacgac ggtggcgcgg acgcctgagg ccgacgcctc
     3301 ggctctgact cgctgcttcg gcggcgatcg gcaccccttt gaagaggctt ggcagcgagc
     3361 gcccctgacc ggccacgacc
//
LOCUS       DASTFV010000056         4340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_187037, whole genome shotgun sequence.
ACCESSION   DASTFV010000056 DASTFV010000000
VERSION     DASTFV010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187037"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..827
                     /locus_tag="VFP02_01015"
     CDS             174..827
                     /locus_tag="VFP02_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET9541630.1"
                     /translation="MSGSRTLPEDESKRLLAGYGIPFAPEIVAEDVAAASAAATEIGF
                     PVVAKLNGEGIAHKTERGLVRLNLSDAEAVETATTELLAAARPGDRAVSVLIAPFLAA
                     SRELIAGIHRDPQFGPTVMLGIGGILAEALADVAFRLAPIERVDAADMVDQLRTQALL
                     GEVRGEPAVDRTAVEDVLLALSAVAVERDDVASADLNPLLVVDGKPIAVDALVEVST"
     gene            824..2290
                     /locus_tag="VFP02_01020"
     CDS             824..2290
                     /locus_tag="VFP02_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET9541631.1"
                     /translation="MSERDPFLALFEPRGIVVAGASTHPGKFGFVALHNILSAGYQGK
                     VFATNREGAEVLGVPTVADVDDLPDGEIDLVFVCTPAAANPALLRACATKGIRAAFVA
                     SAGYREAGPEGRRAEDDLVALAAELGILLVGPNGQGLISTPADLCAQIVAPYPPAGRI
                     GIASQSGNFVSSFMNWAQLSGIGVSRAVSAGNSAAVDVPDFLEFYARDPATAVGLSYV
                     EGIEDGRAFYDRTRGVVAQKPLVVVKGGVTSSGAAAASSHTGALAADDAVFDGACRQA
                     GITRAATIEEAYEAAATFATQPLPKGPRVAVYTTAGGWGVVTADAIARSSLELLALPD
                     DLKAEIDQHLPPRWSRNNPIDLAGSETRDTIPRVMEIVVNHRDVDAVIFLGLGIQSNL
                     ARMEREGRFYPDHGLERIVEYHERQDARFAQAAAELSEASGKPILTATELAVSDPTNA
                     GPAAVRATGRLCYASSNRAVTALDHLWRYARYRRRRGL"
     gene            2298..3716
                     /gene="dacB"
                     /locus_tag="VFP02_01025"
     CDS             2298..3716
                     /gene="dacB"
                     /locus_tag="VFP02_01025"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HET9541632.1"
                     /translation="MRRLAVPLLLVVVALGCGIGAVYADTGGETAEAAVAIESPVTPV
                     LSARRVPEHMTEDIADRRLTTALTGLVDGAPPDTCLVVDAGGRPIIAHQAGTGLIPAS
                     NEKVLVAAAALDELGPRQRFTTTVKAASEPNRQGVVAGDLWLVGGGDPLLTTRRYAGT
                     SDSQAEAHTALEDLADQVVDAGVTQVQGQVLGDDSRYDSTRYVGSWPSRFIDQNQTGP
                     LSALSVNDGFSSFPSAADPGAPETPSANPPALAAQAFLDLLTRRGVRVSGTAGTGTAP
                     RQAPVVAEVRSAPLRDITAQLLTWSDNQTAELLLKEIAVARGGPGTTAAGADAVEAIL
                     TDAGFDLAGSDPEDGSGLSGSNRVTCGLLHQVLADAGPRSALVRGLAIAGQTGTLAST
                     FNGTPAEGRLRAKTGSLNEARALSGVVTVPDGDDLTFSLLVNQAVITPEGDGVRVDVG
                     LALADYPRRPGLDEVGPRPLAR"
     gene            3731..4327
                     /locus_tag="VFP02_01030"
     CDS             3731..4327
                     /locus_tag="VFP02_01030"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET9541633.1"
                     /translation="MPMFPLGTVLFPTMLLPLHVFEPRYRALVDDCVAGDGEFGVTLI
                     ERGSEVGGGDVRTDVGTVARIVQVERFEDGRFAVAAVGTRRIRIERWLADDPYPRAEV
                     VDWEDVGRTETALDDADALFRRALALAAELGEAPAPLDVELADDPAMATFQIGALAPL
                     GPLDRQALLATERPDDRVTLLVAQLTDVVELLRARLGR"
BASE COUNT          572 a         1683 c         1484 g          601 t
ORIGIN      
        1 cgctcccgtg ctggtcggca tgctcggcaa ggaccccctg agcgacgagg ccctggccgc
       61 ccggctcgag cacgtccgtc gcttcttccg cgccgccctc gaaccctgag ccgacccgtg
      121 cacccggccg ggtggacctg cgaaccctga gccaggccct accgttctcg cccgtgtccg
      181 gctcccgcac cctgcccgag gacgagtcca agcgcctcct ggcgggctac ggcatcccgt
      241 tcgcccccga gatcgtcgcc gaagacgtcg ccgccgccag tgccgccgcg accgagatcg
      301 gcttccccgt ggtggccaag ctgaacgggg agggcatcgc ccacaagacc gagcgcgggc
      361 tggtccggct gaacctgtcc gatgcagagg cggtcgagac cgcgaccacc gagctcctgg
      421 ccgcagcccg tcccggggac cgggcggtct cggtgctgat cgccccgttc ctggcggcca
      481 gtcgggagct gatcgcgggc atccaccgcg atccccagtt cggaccgacg gtgatgctgg
      541 gcatcggcgg gatcctggcc gaggctctcg ccgacgtcgc cttccggctg gccccgatcg
      601 agcgggtcga cgccgccgac atggtcgacc agctccgcac gcaggccctg ctgggcgagg
      661 tccggggcga gcccgcggtg gaccgcaccg cggtcgagga cgtcctgctc gccctctccg
      721 ccgtcgccgt cgagcgtgac gacgtggcct cggctgacct caacccgctc ctcgtcgtcg
      781 acgggaagcc gatcgccgtc gacgccctcg tggaggtgtc gacgtgagcg agcgcgaccc
      841 cttcctcgcg ctcttcgagc cccgggggat cgtggtcgcc ggggcgtcga cccaccccgg
      901 caagttcggg ttcgtggccc tccacaacat cctcagcgcc ggctatcagg gcaaggtctt
      961 cgccaccaac cgggagggag ccgaggtcct cggcgtcccg acggtggccg acgtcgacga
     1021 tctcccggac ggcgagatcg acttggtctt cgtctgcacc ccggcggccg ccaaccccgc
     1081 cctcctgagg gcatgtgcca ccaaaggcat ccgggccgcc ttcgtggcct cggccggcta
     1141 ccgggaggcc ggccccgagg gtcggcgggc cgaggacgac ctggtcgccc tcgcggccga
     1201 gttggggatc ctgctggtgg gccccaacgg tcagggtctc atctccacgc ctgcggacct
     1261 ctgtgcgcag atcgtcgccc cctatccgcc ggccggccgc atcggcatcg ccagccagtc
     1321 gggcaacttc gtgtcgtcgt tcatgaactg ggcccagctc tccggcatcg gcgtgagccg
     1381 ggccgtcagc gccggcaact ccgcggcggt cgacgtgccc gacttcctcg agttctatgc
     1441 ccgcgacccg gcgaccgccg tcggcctgtc ctacgtcgag ggcatcgagg acggccgcgc
     1501 gttctacgac cgcacgcgcg gcgtcgtcgc ccagaagccc ctcgtggtgg tgaaaggtgg
     1561 ggtcacctcc agcggggcgg ctgccgcgtc gagccacacc ggcgccctgg ccgccgacga
     1621 cgccgtgttc gacggggcct gccgccaggc gggcatcacc cgggccgcca ccatcgagga
     1681 ggcctacgag gctgccgcca ccttcgccac ccagcccctg cccaagggcc cgcgggtcgc
     1741 cgtctacacg accgccggag gctggggcgt ggtcaccgcc gacgccatcg cccgctccag
     1801 cctggagctg ctggccctgc ccgacgacct caaggccgag atcgaccagc acctgccgcc
     1861 tcgctggagc cgcaacaacc cgatcgacct ggccggtagc gaaacccgcg acaccattcc
     1921 ccgggtgatg gagatcgtgg tcaaccaccg cgacgtcgac gccgtcatct tcctcggcct
     1981 cggcatccag tcgaacctgg ctcgcatgga gcgcgaaggt cgcttctacc cggaccacgg
     2041 cctcgagcgc atcgtcgagt accacgagcg ccaagacgcc cggttcgctc aggccgcggc
     2101 ggagctgtcc gaggcctcgg gcaagccgat cctgaccgct acggagctgg cggtgtccga
     2161 cccgaccaac gccggtccgg cggcggtgcg ggccacgggt cgcctctgct acgcctcctc
     2221 caaccgggcg gtgaccgccc tcgaccacct ctggcgctac gcccgctacc gccgacgccg
     2281 ggggctctga ccgactcatg cgccgactcg ccgtccccct gctgctggtg gtcgtcgccc
     2341 tgggatgcgg gatcggcgcc gtctacgccg acacgggcgg cgagaccgca gaagcggcgg
     2401 tggccatcga gtcgccggtg actccggtgc tctcggcccg gcgagtcccc gagcacatga
     2461 ccgaggacat cgccgaccgc cgcctcacga cggccctgac cggcctggtc gacggggccc
     2521 cacccgacac ctgcctggtc gtggacgccg ggggccggcc gatcatcgcc caccaggccg
     2581 gcacggggct gatccccgcc tccaacgaga aggtcctcgt ggcggccgcg gcgctggacg
     2641 agctcgggcc gcgccagcgc ttcaccacga cggtcaaggc cgcgtccgag ccgaaccgac
     2701 agggtgtcgt cgccggtgac ctctggttgg tcgggggtgg tgatccgctt ctcaccaccc
     2761 ggcgctacgc cggcacgtcg gacagccagg ccgaggccca caccgcgctg gaagacctgg
     2821 ccgaccaggt cgtcgacgcc ggcgtgacgc aggtccaggg ccaggtcctg ggcgacgaca
     2881 gccgctacga cagcactcgc tacgtgggca gctggccgtc ccgcttcatc gaccagaacc
     2941 agaccggccc gctctcggcc ctctcggtga acgacggctt ctcctcgttc ccgtcggccg
     3001 cggaccccgg tgcgcccgag acgccgtcgg ccaacccacc ggcgctggcc gcgcaggcgt
     3061 tcctcgatct gctcacccgg cgaggcgttc gggtctcggg tacggccggg accgggaccg
     3121 cccccaggca ggccccggtc gtggccgagg tgcgctcggc cccgttgcga gacatcaccg
     3181 cgcagctcct gacctggtcc gacaaccaga ccgccgagct gttgctgaag gagatcgcgg
     3241 tggctcgggg cgggccgggc acgaccgccg ccggcgcgga cgccgtggag gcgatcctca
     3301 ccgatgccgg cttcgacctg gccgggagcg atcccgagga cggatcaggc ctgtcgggca
     3361 gcaaccgggt cacctgtggc ctgctccacc aggtcctggc cgacgccggg ccccggtccg
     3421 ccctcgtgcg gggcctggcc atcgccggcc agaccggcac cttggcctcc acattcaacg
     3481 ggacgccggc cgagggtcgc ctccgcgcca agaccggctc gctcaacgag gcccgggccc
     3541 tctccggcgt ggtgaccgtg cccgacggcg acgacctgac cttctcgctt ctcgtcaacc
     3601 aggccgtgat caccccggag ggcgacggcg tgcgcgtcga cgtcgggctg gccctcgccg
     3661 actacccccg acggcctggc ctcgacgagg tcggtccgcg gcccctcgcc cggtgacgac
     3721 gccgagcgtg atgcccatgt tccccttggg caccgtgctg ttcccgacga tgctcctgcc
     3781 cctgcacgtc ttcgagcccc ggtaccgggc cctggtcgac gactgcgtcg ccggtgacgg
     3841 cgagttcggc gtgaccctca tcgagcgggg gagcgaggtc ggtgggggcg acgtgcgcac
     3901 cgacgtgggc accgtcgccc ggatcgtgca ggtcgaacgc ttcgaggacg gtcgcttcgc
     3961 ggtcgccgcc gtggggaccc gacgcatccg catcgaacgc tggttggccg acgatcccta
     4021 tccccgcgcc gaggtcgtcg actgggagga tgtggggaga accgaaaccg cgttggacga
     4081 cgccgacgcc ctcttccgtc gggccctggc gctcgccgcg gagttggggg aggcaccggc
     4141 gccactcgac gtcgagctcg ccgacgaccc ggcgatggcc accttccaga tcggcgccct
     4201 cgccccgctc ggccccctcg atcggcaggc gctgctggcc actgagcggc ccgacgaccg
     4261 ggtcaccctg ctcgtcgcgc agctgaccga cgtggtggaa ctactccggg ccaggctcgg
     4321 gaggtagaaa ggtcccgtgc
//
LOCUS       DASTFV010000057         3054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_195362, whole genome shotgun sequence.
ACCESSION   DASTFV010000057 DASTFV010000000
VERSION     DASTFV010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195362"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFP02_01035"
     CDS             <1..308
                     /locus_tag="VFP02_01035"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET9541634.1"
                     /translation="LGEDHHTPWGIVHGGVYATAIESAASIGASTAVRERGMVAVGLT
                     NTTHFLRSVAAGRVLVEAEALHQGRSQQLWRVDVRDDRDRLLAHGEVRLQNLDPGAG"
     gene            326..1306
                     /locus_tag="VFP02_01040"
     CDS             326..1306
                     /locus_tag="VFP02_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9541635.1"
                     /translation="MTLVPPAAAIPPPPLGPTASLLRTTALTHTYPDGTTAITDLAIE
                     VPRGSVGLVGANGAGKTTLFRLMLGQMFPTRGAIEVVGLPVADDPVGIRSRLGYMPEH
                     DCLPNDQSAADLVATFGELAGLPAQAARQRASDVLDLVGLDEARFRPVGGFSTGMRQR
                     AKLAQALVADPELVLLDEPTAGLDPVGREEMLDLVGRLADFGISVLFATHLLDDVQRV
                     CDHVVMIDAGRLVVAGPIEALVERTGVLRVEVGGRADGIEALVSALAERSVAAVAVDA
                     HIVDVDISGHGDEVLDVVRDLVADLGLRLYALSSRHHSLDELFVEAAQRP"
     gene            1303..2223
                     /locus_tag="VFP02_01045"
     CDS             1303..2223
                     /locus_tag="VFP02_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541636.1"
                     /translation="MSAVAPPALGGAVYDRGYRPYEGARGGRSAGRWALWRLTLRRAL
                     GLRRSWRQKVLPWTLLGLVTIPAAVDLGVRYATRNTPLSEIGYEFLTFRDYVGVSTTL
                     LLFVAVSAPDVICPDRHDRVLPLIFSRPLTGDDYVLAKVGGIFALVFGFSFFPQVLLY
                     IGQMLVNEDGSLTYVGDHLDVLWQVPVSVAILALYYSVVSVAVASLTDRRIVGGLAFL
                     GVLFIPAIIAGAIVEATNEGEGTIFGVFNLLNLPLMLRDVVFEGQIDVDRDGGLSGVA
                     GAGWAALLLYVAIVGTGVFVLHRRYREARL"
     gene            2220..>3054
                     /locus_tag="VFP02_01050"
     CDS             2220..>3054
                     /locus_tag="VFP02_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9541637.1"
                     /translation="MSAPEPQATPETDPAFVPHPTVEVAGVSVWFGPKVAVTELSCSF
                     GPGVTGLLGPNGAGKTTVMRAICGLLPTDRGAIRVLGRDPRHDRSVQQDISLVPEDDA
                     VPGALTARQLVRYAASLHSVRDPIAPEASLAAVGLLDVADRRVAGFSKGMRQRAKIAA
                     SLVTQPKVMVLDEPLNGADPVQRAALIGLFQTLGAQGRTVIISSHVLNEVERLASRQL
                     VVIRGRLAAAGGHRAIRDALADRPRSVLVRTTEPRRLAGDLLASEAVQGVSVDGDKLV
                     IS"
BASE COUNT          350 a         1170 c         1056 g          478 t
ORIGIN      
        1 acctgggcga ggatcaccac accccttggg gcatcgtcca cggcggggtc tacgccactg
       61 ccatcgagtc ggcggccagc atcggggcca gcaccgcggt ccgggagcgg gggatggtcg
      121 ccgtcgggct gaccaacacc acgcacttcc tgcgctcggt cgccgcggga cgcgtgttgg
      181 tcgaggccga ggcgctccac cagggtcgga gccagcagct ctggcgggtc gacgtccgcg
      241 acgaccgcga ccggctgctg gcccacggcg aggtccggct ccagaacctg gaccccggcg
      301 ccggttgacc tagcctcggg gggacatgac gctggtcccc cctgccgcgg cgatcccgcc
      361 gcccccgctc gggccgacgg catcgctcct gcggacgacg gcgctgaccc acacctatcc
      421 ggacgggacc acggcgatca ccgacctcgc catcgaggtg cctcgcggct cggtcggcct
      481 cgtcggggcc aacggcgccg gcaagacgac cctcttccgg ctgatgctgg gccagatgtt
      541 cccgacccgc ggggccatcg aggtggtcgg cctgccggtg gccgacgacc cggtcgggat
      601 ccgcagccgc ctcggctaca tgcccgagca cgactgcctg cccaacgacc agtccgcggc
      661 cgacctcgtc gccacgttcg gtgagctggc cgggctgccg gcccaggccg cccgccagcg
      721 ggcctccgac gtgctcgacc tcgtcggcct ggacgaggct cggttccgcc ccgtcggcgg
      781 cttctcgacg gggatgcgcc aacgggcgaa gctggcgcag gccctcgtgg ccgaccccga
      841 gctggtcctg ctcgacgagc cgacggcggg cctcgacccc gtcggtcggg aggagatgct
      901 cgacctcgtc ggccgtctcg ccgacttcgg catctcggtc ctcttcgcca cccacctcct
      961 cgacgacgtg cagcgggtct gtgaccacgt cgtgatgatc gatgcgggcc gcctggtcgt
     1021 cgccggtccc atcgaggccc tggtcgagcg caccggcgtc ctccgggtgg aggtcggcgg
     1081 tcgggccgac gggatcgagg ccctggtctc ggccctggcg gagcggtccg tcgcggcggt
     1141 cgccgtcgac gcccacatcg tcgacgtcga catctccggc cacggtgacg aggtgctcga
     1201 cgtcgttcgg gacctcgtcg ccgacctcgg cctccgcctc tacgccctgt ccagccggca
     1261 ccactccctc gacgagctct tcgtcgaggc ggcccagcgt ccgtgagcgc cgtcgccccg
     1321 cccgcgctcg ggggtgcggt ctacgaccgc gggtaccggc cctatgaggg ggcccggggg
     1381 ggtcgttcgg ccgggcggtg ggcgctgtgg cgcctcaccc tgcgccgggc cctcggcctg
     1441 cgccgctcct ggcgccagaa ggtcctgccc tggaccctgc tcgggctggt cacgatcccg
     1501 gcggcggtcg acctgggtgt ccgctacgcc acccggaaca cgccgctgag cgagatcggg
     1561 tacgagttcc tgacgttccg ggactacgtg ggcgtctcca cgacgctgct gctgttcgtg
     1621 gccgtcagcg cgcccgacgt gatctgcccg gaccgccacg accgggtcct gcccctgatc
     1681 ttctcgcgtc ccctcaccgg cgacgactac gtgctggcga aggtcggggg gatcttcgcg
     1741 ctggtcttcg ggttctcctt cttcccccag gtcctcctct acatcggcca gatgctggtc
     1801 aacgaggacg gatcgctcac ctacgtcggc gaccacctcg acgtcctgtg gcaggtcccg
     1861 gtgtcggtcg ccatcctggc cctctactac tcggtggtga gcgtggccgt ggcctcgctc
     1921 accgatcgcc ggatcgtcgg cggcctggcc tttctcgggg tgctcttcat cccggccatc
     1981 atcgccggcg ccatcgtcga ggcgaccaac gaaggtgagg gcacgatctt cggggtcttc
     2041 aacctgctca acctgccgct gatgctgcgg gacgtcgtct tcgagggcca gatcgacgtc
     2101 gaccgggacg gcgggctctc gggcgtggcc ggagcgggct gggccgccct gctcctctac
     2161 gtcgccatcg tcgggactgg tgtgttcgtc ctgcaccgcc gctaccggga ggcccgtctg
     2221 tgagcgcgcc ggagccgcag gcgacccccg agacggatcc cgccttcgtc ccgcacccca
     2281 ccgtcgaggt cgccggcgtt tcggtctggt tcggcccgaa ggtcgccgtc accgagctgt
     2341 cgtgctcgtt cggtccgggc gtgaccgggt tgctgggccc gaacggcgcg ggcaagacca
     2401 ccgtgatgcg ggccatctgc ggcctgttgc cgaccgatcg cggggcgatc cgcgtgctcg
     2461 ggcgcgaccc ccgtcacgac cggtcggtgc aacaggacat ctccctcgtg cccgaggacg
     2521 acgccgtgcc cggggccctg accgcccgcc agctcgttcg ctacgccgcc tcgctccact
     2581 cggtgcggga cccgatcgcc cccgaggcct ccttggccgc ggtcgggctc ctcgacgtgg
     2641 ctgaccgccg ggtggccggc ttcagcaagg gcatgcgcca gcgggccaag atcgccgcgt
     2701 ccctggtcac ccagcccaag gtgatggtcc tcgacgagcc gctgaacggc gccgacccgg
     2761 tgcagcgggc cgccctgatc gggctcttcc agaccctcgg ggcccagggc cgcaccgtca
     2821 tcatcagctc ccacgtcctc aacgaggtcg agcgcctggc ctcccggcag ctggtggtca
     2881 tccggggccg cctcgccgcc gcgggcggtc accgcgccat ccgcgacgcc ctggccgatc
     2941 ggccccggtc cgtcctggtg cgcacgaccg agccccgccg cctggccggc gatctcctgg
     3001 ccagcgaggc ggtccagggc gtgtccgtgg acggcgacaa gctggtcatc tcct
//
LOCUS       DASTFV010000058         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_197939, whole genome shotgun sequence.
ACCESSION   DASTFV010000058 DASTFV010000000
VERSION     DASTFV010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197939"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VFP02_01055"
     CDS             complement(<1..508)
                     /locus_tag="VFP02_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9541638.1"
                     /translation="MTIPPLPPGGPVALPGRGTTFHRDLPSRAGADAPTVILLHGWTA
                     TADLNWFPAYEALAEHCRVVALDHRGHGQGIRTARRFRLADCADDAVALADVLGIDRF
                     IPVGYSMGGLIAQLTWHRHPDRVGGLVLGATARNFRGNRAAAGGFTALGAMAAAARLV
                     PSSVRAGAA"
     gene            complement(511..981)
                     /gene="greA"
                     /locus_tag="VFP02_01060"
     CDS             complement(511..981)
                     /gene="greA"
                     /locus_tag="VFP02_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET9541639.1"
                     /translation="MAENIQLTQATFDRLKEEHGHLTTVWRIDIAKRIEAARELGDLK
                     ENGDYHAAKEEQGQTEARIRDLEAMIERAEIVEGGSDDGTVAAGSVVSIRYEGDDDVE
                     TYLLGSIEEKRDGIEIMSPGSPIGQALVGAQAGDVVEAETPGGTLRVEIVSVGA"
     gene            1281..>2085
                     /locus_tag="VFP02_01065"
     CDS             1281..>2085
                     /locus_tag="VFP02_01065"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET9541640.1"
                     /translation="MTHRIGVIGGDGIGPEVVAEGLKVVRAAGVELDTVDYDLGGARY
                     LRDGVVLPDEVVEEWRGLDALFLGAVGTPDVPPGVIERGLLLRMRFALDLYVNLRPFL
                     HPPTIDFLVVRENTEGAYAGEGGFLRKDTPHEIATQGSVNTRMGVERAVRFAFDLARS
                     RERRHLTLVHKTNVLTFAGDLWERAFDAVAAEYDDITTAYQHVDAACIHFVQDPGRYD
                     VIVTDNLFGDILTDLAAAVTGGIGLAASGNLDVTRRNPSMFEPVHGSAPD"
BASE COUNT          293 a          734 c          750 g          308 t
ORIGIN      
        1 gggccgcgcc ggcccggacc gaggagggaa ccaggcgggc ggcggcggcc atcgccccca
       61 gcgcggtgaa ccctccggcg gcggcccggt tgccgcggaa gttgcgggca gtggctccca
      121 gtacgaggcc accgacccgg tcggggtgcc ggtgccaggt gagctgggcg atcagcccac
      181 ccatcgaata gccgaccggg atgaagcggt cgatgccgag cacgtcggcc agggccacgg
      241 cgtcgtcggc gcagtcggcc aggcggaagc ggcgggcggt gcggatgccc tggccgtgac
      301 cccgatggtc gagggccacg acccgacagt gctcggccag ggcctcgtag gccgggaacc
      361 agttcaggtc ggccgtagcc gtccatccgt ggaggaggat gacggtcggg gcgtcggccc
      421 cggcccgact ggggaggtcg cggtggaacg tggtcccccg cccgggcagc gcgacgggcc
      481 caccaggagg gaggggcggg atggtgatgg ctacgccccc accgagacga tctcgacgcg
      541 cagggtcccg ccgggggtct cggcctcgac gacgtcgccg gcctgggccc cgacgagcgc
      601 ctggccgatg ggtgagccgg gcgacatgat ctcgatgccg tcccgcttct cctcgatcga
      661 cccgagcagg taggtctcga cgtcgtcgtc accctcgtag cggatcgaca ccaccgatcc
      721 ggcggccacc gtgccgtcgt cggacccgcc ctcgacgatc tcggcccgct cgatcatggc
      781 ctcgaggtca cggatgcggg cctcggtctg gccctgctcc tccttggcgg cgtggtagtc
      841 gccgttctcc ttgaggtcgc ccagttcgcg ggcggcttcg atgcgcttgg cgatgtcgat
      901 gcgccagacc gtggtcaggt gcccgtgctc ttccttcagc cggtcgaacg tggcctgcgt
      961 gagctggatg ttctccgcca tggggagagg ttaagggtca gggatgagcg gggctaccgc
     1021 ccaaccggac gacaggtgct ggtccccggt gtgggtatcg aggttgacga tcgggtcccg
     1081 aggcccgaga ctggcggccg tgaagcgcca cctcgcgccc atcgtcgtaa ttacctagcg
     1141 cgcgccgatc tcggcccgcg ctcctcgacc gtccacctcg gtgggcggtt tcttcgttct
     1201 gggacggccc cgccgaccgg aacatcgacc aggtccagct gtgcagcgag aggtagtccc
     1261 caagtgacaa ggtcagcgac atgactcacc gcatcggcgt catcggcggc gacggcatcg
     1321 gccccgaggt cgtggccgag ggtctgaagg tcgtgcgcgc ggccggcgtg gagctggaca
     1381 cggtcgacta cgacctcggc ggcgcccgct acctgcggga tggtgtcgtg ctccccgacg
     1441 aggtcgtgga ggagtggcgg ggcctcgacg ccctcttcct cggcgcggtc ggcacgcccg
     1501 acgtgccgcc gggggtcatc gagcgggggc tgctgctcag gatgcgcttc gccctggacc
     1561 tctacgtcaa cctgcggccg ttcctgcacc cgccgacgat cgacttcctc gtcgtgcgcg
     1621 agaacaccga gggggcctac gccggcgagg gcggcttcct gcgcaaggac acgccccacg
     1681 agatcgcgac ccagggatcg gtcaacaccc ggatgggcgt cgagcgcgcc gtccggttcg
     1741 ccttcgacct ggcccgcagt cgggagcgcc ggcatctcac cttggtccac aagaccaatg
     1801 tgctgacctt cgccggtgac ctctgggagc gggcgttcga cgcggtggcc gccgagtacg
     1861 acgacatcac cacggcctac cagcacgtcg acgccgcctg catccacttc gtgcaggatc
     1921 ccggccggta cgacgtgatc gtcaccgaca acctgttcgg cgacatcctc accgacctcg
     1981 ccgccgccgt caccggcggc atcggcctgg ccgccagcgg caacctggac gtgacccggc
     2041 gcaacccgag catgttcgag ccggtgcacg gcagcgcccc ggaca
//
LOCUS       DASTFV010000059         6750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_198225, whole genome shotgun sequence.
ACCESSION   DASTFV010000059 DASTFV010000000
VERSION     DASTFV010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198225"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFP02_01070"
     CDS             complement(<1..450)
                     /locus_tag="VFP02_01070"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HET9541641.1"
                     /translation="MRRLFLVLILLAPLSACGGSDEADCGPVTREALDPNAGLHVLPD
                     QDQPRYTTDPPTSGAHYSLPPPTGVQDDPIERPMQVTALEGGQVLVQYRDLAAADLAR
                     LEDLASDVVVVAPNPDQAERVVLTAWITKQTCSGVDVDTVATFIDEQA"
     gene            510..797
                     /locus_tag="VFP02_01075"
     CDS             510..797
                     /locus_tag="VFP02_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541642.1"
                     /translation="MRRSTVERRLRDNSRRLQLLREELGITAEQMTQLGEEAEDARIR
                     AMVSETALAQREHSDAERHAAALSRHRAEVEAEIERLEQDQDRLLDQLGGG"
     gene            879..1463
                     /locus_tag="VFP02_01080"
     CDS             879..1463
                     /locus_tag="VFP02_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9541643.1"
                     /translation="MSTRVVIAEDEAIIRLDLKETLEEEGYEVVGETGRGDEAVDLVK
                     ELEPDIAILDIKMPGLDGLSAAREIAGERRAAVLILTAFSQRDLIEKARDAGALAYLV
                     KPFQRSELVPALEVALGRFQEMRALHEQAESLEEQLETRKVVDRAKGQLMDRQKMNES
                     DAFAFIQKTAMHRRHTMKRVAQDIIDGELTPEAS"
     gene            1511..4177
                     /gene="polA"
                     /locus_tag="VFP02_01085"
                     /pseudo
     CDS             1511..4177
                     /gene="polA"
                     /locus_tag="VFP02_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
     gene            4232..4411
                     /locus_tag="VFP02_01090"
     CDS             4232..4411
                     /locus_tag="VFP02_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541644.1"
                     /translation="MSTKRATRASIWFCLALALAIYVLVSASLAVATADVCDGTLGGQ
                     KTWQFAPPHWECPGR"
     gene            4461..5165
                     /locus_tag="VFP02_01095"
     CDS             4461..5165
                     /locus_tag="VFP02_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9541645.1"
                     /translation="MGAAYERYSFTKGTAQEAAFLVEALALTPGERVLDLGCATGRHV
                     RALAGHGVAPIGLDVSARFLAVAAEEAPELPFVRGDARNLPIADGTVDAVVSLCQGGF
                     GLTGGPAAAGLAAGVEPDEVVLAELSRVLRPGGRLALTAFSSYFAVRDLGDGEDFDAG
                     SGVLHEHTVVHDPEGRPAEQELWTTCYTPRELRLLLDRCGFSVAGLWSVRPGRYGRDD
                     PTVDSPEYLAVGRKRR"
     gene            5242..>6750
                     /gene="rpsA"
                     /locus_tag="VFP02_01100"
     CDS             5242..>6750
                     /gene="rpsA"
                     /locus_tag="VFP02_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET9541646.1"
                     /translation="MSDDLTPSATALADPPAADDSPFGTFDEEGVYHPRQIVDNDLGA
                     GLDEAYSATMVEVKDGQLVSGTVVRVDHDEVLLDIGYKSEGVIPSRELSIRNDVDPSE
                     IVSLGDSLDALVLTKEDKDGRLILSKKRAQYELAWGKIEEIKEAEGMVSGPVIEVVKG
                     GLILDIGLRGFLPASLVELRRVRDLQPYVGKVLEAKIIELDKNRNNVVLSRRAWLEET
                     QREQREEFLENLKPGERRKGVVSSVVNFGAFVDLGGMDGLVHVSELSWKHVDHPSSVV
                     AVGDEIEVQVLEVDLDRERISLSLKATQQDPWEEFASGHRVGELVYGRVTKLVPFGAF
                     VQVGDGIEGLVHISEMSAHHVELPEQVVTPGEELWVKVIDLDLQRRRISLSIKQAAEG
                     GVVAAEYQEHFGEHAYDESGNYVGPSGEIDEAWAEYYEEHGLDPATGQPKVPTATPAT
                     EEDAAAGDSNGAMAAALADAGLEAPTEPEDAEAPADPEAEVAEAPAEPDGAEA"
BASE COUNT         1065 a         2478 c         2292 g          915 t
ORIGIN      
        1 cgcttgttcg tcgatgaagg tcgccaccgt gtccacgtcc acgccggagc aggtctgctt
       61 ggtgatccag gcggtcagca cgacgcgctc ggcctggtcg gggttgggcg ccaccaccac
      121 gacgtcggac gccaggtcct ccaaccgggc caagtcggcg gccgcgaggt cccggtactg
      181 gacgaggacc tgaccgccct cgagggcggt cacctgcatc gggcgctcga tcggatcgtc
      241 ctggaccccg gtgggcggcg gcagtgagta gtgggcgccg gaggtgggcg ggtcggtggt
      301 gtacctcggc tggtcctggt cggggaggac gtgcagaccc gcgttcgggt cgagcgcctc
      361 cctggtgacc ggaccacagt cggcctcgtc cgagcccccg cacgcgctca ggggcgccag
      421 caggatcagg accaggaaca ggcgccgcac gccgtcatga tgcccggggc gtcacctccg
      481 cccgccagtc ggcctcgtag gatcgggccg tgcgccggtc gaccgtggag cgacggctcc
      541 gggacaactc ccgccgcctg cagctgctcc gcgaggagct gggcatcacc gccgagcaga
      601 tgacccagct cggcgaggag gccgaggacg cccgcatccg agccatggtc tcggagaccg
      661 cactggccca gcgcgagcac agcgacgccg agcgccacgc ggccgccctg tcccggcacc
      721 gggctgaggt ggaggccgag atcgagcgcc tggagcagga ccaggaccgt ctgctggacc
      781 agctcggcgg tggctgagcc accagtcgcg gcccgaccgg gatcggcaag tacagtcgcc
      841 ggttcgcgag cgagagccat ctgaggagcc ccgaacccgt gtccacgcgt gtcgtcatcg
      901 ccgaggacga agccatcatc cgcctggacc tcaaggagac cctggaggaa gagggctacg
      961 aggtcgtcgg cgagaccggt cggggcgacg aagccgtcga cctcgtcaag gagctcgagc
     1021 ccgacatcgc catcctggac atcaagatgc cggggctgga cggcctgtcc gccgcccggg
     1081 agatcgccgg cgagcgccgg gcggccgtgc tcatcctcac cgcgttcagc cagcgggacc
     1141 tgatcgagaa ggcccgcgac gccggcgccc tggcctacct ggtcaagccg ttccagcgca
     1201 gcgagctggt gcccgccctc gaggtggccc tgggccgctt ccaggagatg cgggccctcc
     1261 acgagcaggc cgagtccctc gaagagcaac tggagacccg caaggtggtg gaccgggcca
     1321 agggccagct catggaccgc cagaagatga atgagtccga cgccttcgcc ttcatccaga
     1381 agacggccat gcaccgtcgc cacaccatga agcgcgtggc ccaggacatc atcgacgggg
     1441 aactgacacc ggaagcgagc tgagcatgcc ggacccctcg gtgggcgcga cgccggccac
     1501 caacaaggtc atgctcgtcg acggcaactc gctgacctac cgggccttct tcgcccttcc
     1561 caccgacatg gccactgcct ccggccaggt caccaacgcc gtcttcggct tcacgtcgat
     1621 gatgatcaac ctgatccggg accaccagcc caccaacgtg gtggtggcgt tcgaccgccc
     1681 ggagccgacg ttccggcacg agcgcgtcgc cacctacaag ggcaaccggg acaccgcccc
     1741 cgacatcctg cgccagcaga tgggcctcgt ccgccaggtc ctcgaggcgc tgcgcatccc
     1801 catcctcgag gcacccggct acgaggccga cgacatcctg gccacccttg ccacccgggc
     1861 ccgggacgca ggtgacgagg tgctcatcgt caccggtgat cgggacacct accagttggt
     1921 cgaggacccc ctgatccggg tgatctacaa caagcggggt gtgtcggact acgccgacta
     1981 cgacgaggcc ggcatcaagg agcgcacggg ggtggcgccg accgactacg tgatctacgc
     2041 cgccctccgg ggcgatccgt cggacaacct cccggggatc cccggtgtgg gcgagaagac
     2101 ggcagccaag ctcgtcaaca cgtacggcga tctcgacggg atcttctccc acctcgacga
     2161 ccagaccccc aagctgcgcc agaacctggc cgagaacgag gcccaggccc gcctgaacgc
     2221 cgaggtcatg gtcctgctcc gggacatccc cctcgaggtc cagctcgacg acctcaccat
     2281 ggggaccttc gacaccgaag aggtgcggaa gctcttcgac ttcctcgagt tccgcaccct
     2341 cttcgaccgc ctggccgaag cgttcgacaa cgacctgggc ccggccctcg ggggctccga
     2401 ggtgctggag gcggaggtcg tcgagaccga gacgatcgcc gacgcgatca ccgtgctcaa
     2461 cggcctggcc gccgccgacc gcctcgtggc ggtggccggc gcctggcgcg gcgcccccgg
     2521 ccgcagcccg ctggagggct tggccctggt gaccgagccg gccgccgcgg aggtgacgtg
     2581 gatcccggcc gagctgctgg ccgacgccga ggtccgcgac gcgctcgcgg ccaccgcgcc
     2641 gacagacggc cgccgcctgg cggcccatgg ggcgaagccg ctcctgcgga gcctcaccga
     2701 gctcggggtc gacgccgggc tcggcgtcga cacgatgctg gcggcctacc tgctcgaccc
     2761 ggccgagtcc cgctacctgc tcgaagaact gctgatccgg tacgcccagc tccagctccc
     2821 cgagcagtcg gccagcgccg agggccagct cgacctcgac gagaccgcca cgtcgcccgc
     2881 ggtcacagcc ggccgggagg ccttggcggt cgaccgcctg atcgcccccc tccaggcgtc
     2941 gatggtggcc aacggcgtcc agcagctctt cgacgacatc gaggtgccgc tggtcggcgt
     3001 gcttgcccgc atggaggcgg tcggcatcgc ggtggaccgc gccgtcctcg aggccatcca
     3061 cgagcagctg gtcaccgaat gcgacgtgct cagcgcccag atcgtcgagg acgcgggcga
     3121 gccgttcaac gtgaactcca ccaagcagat gcgggagatc ctcttcgaca agctgggcct
     3181 caccccacag aagaagacga agaccggcta ctccaccgac gccgccagcc tcgagaagct
     3241 cctcggccag caccccatca tcgagcacct cctgcgctac cgcgaggtcg agaagctgcg
     3301 ggggacctac ggcgaaggcc tcctggccga gatcggcccg gacggccgca tccacgccac
     3361 cttcaaccag acggtggccc gcaccggccg gctcagctcc gacgccccga acctgcacaa
     3421 catcccggtg cgccgcgagg ccggcaagga gttccgcacc gcgttcgtcc ccgcgccggg
     3481 cttcgagatg ctggtggccg actacaacca gatcgagctc cggtgcatcg cccacctggc
     3541 cgaggacccc ggcctgatcg acgcgttcga gagcggtcgg gacatccaca ccgagaccgc
     3601 ctcccggatc ttcggggtgg aggccgacgg cgtcaccggc gagcaacggt cgaaggccaa
     3661 gatggtctcc tacggcctgg cctacggcat ggaggcctac ggctagggtc agcgcctgtc
     3721 gatccccacc gacgaagcgg ccgcgatcct cgacgcctac ttcgtggcct tcccggcggt
     3781 gcgggcctac atggaccaga ccgtggccga ggcccgcgag cggggctaca ccgagaccct
     3841 gttcggacga cggcgcccga tccccgagct gagcagcagc aacttccgca tccgacaggc
     3901 gggggagcgt caggccatga acgccggcat ccagggcctg gccgccgaca tcttcaaggt
     3961 cgccctcgtc cgcctcgacc aggccctgct ggccggcggg cacgagagtc ggctggtcct
     4021 gcaggtccac gacgaggtgc tgctcgaggt ggaccccgcc gagcacgacg ccatgaccga
     4081 gctgaccacg accatcctgt cgggggcggc cgagctccgg gtccccctcg aggtcaatct
     4141 cgccttcggg gacagctggg cggcggcaaa aacgtgatgc cgcgctcaac tccccgcccc
     4201 gtccggccga tggggagggc aggagcggcc gatgtccacc aaacgagcca cccgagccag
     4261 catctggttc tgcttggcgc tagccctcgc catctacgtc ctcgtctccg ccagcctggc
     4321 cgtggcgacg gcggatgtct gtgacgggac cctcggcggg cagaagacct ggcagttcgc
     4381 cccaccccac tgggagtgcc ccggccgctg acgccggcgc tggagggccg acccactggt
     4441 tcgagcccct ggccgaccac gtgggggcgg cctacgagcg ttactccttc accaagggca
     4501 cggcccagga ggccgccttc ctcgtcgagg ccctggccct gacgccgggg gagcgggtcc
     4561 tcgacctcgg ctgcgccacc ggtcgccacg tccgcgccct ggccgggcac ggtgttgctc
     4621 ccatcggcct cgacgtctcg gcccggttcc tggccgtagc cgccgaggag gcgcccgagc
     4681 tgccgttcgt gcggggggac gcccggaacc tgccgatcgc cgacggcacc gttgacgcgg
     4741 tggtgtcgct ctgccaaggg ggcttcggcc tcaccggggg accggctgcc gccgggctgg
     4801 cggccggagt cgagcccgac gaggtggtcc tggccgagct gtcccgggtg ctccgaccgg
     4861 gcgggcgcct ggcgctcacc gccttctctt cgtacttcgc cgtgcgcgat ctgggcgacg
     4921 gggaggactt cgacgccggg agcggcgtcc tgcacgagca cacggtggtg cacgaccccg
     4981 agggccgccc ggccgagcag gagctctgga cgacctgcta cacgccccgg gagctgcgcc
     5041 tgctcctgga ccgctgtggc ttctccgtgg cgggcctgtg gtccgtccgt cccggccgct
     5101 acggccggga cgaccccacg gtcgactctc ctgagtatct cgccgtcggc cgaaagcgcc
     5161 ggtagccttt cagtccgttc cccgggggtg cgcttctgcc ctccggttcc cactgtccag
     5221 cccaacgaaa gtcccctccc cgtgtccgac gacctcaccc cctcggcgac cgctctcgcc
     5281 gatccccccg ccgccgacga cagccccttc ggcaccttcg acgaggaggg cgtgtaccac
     5341 ccccgccaga tcgtggacaa cgatctcggc gccggtctcg acgaggccta ttcggccacc
     5401 atggtcgagg tcaaggacgg ccagctcgtc agcggcaccg tcgtacgggt ggaccacgac
     5461 gaggtcctgc tggacatcgg gtacaagtcc gagggcgtga tccccagccg tgagctgtcg
     5521 atccgcaacg acgtcgaccc ctccgagatc gtctccctgg gcgacagcct cgatgccctg
     5581 gtcctcacca aggaggacaa ggacggccgc ctgatcctgt ccaagaagcg ggcccagtac
     5641 gagctggcct ggggcaagat cgaggagatc aaggaggccg agggcatggt ctccggcccg
     5701 gtcatcgagg tcgtcaaggg tggcctgatc ctcgacatcg gcctgcgggg cttcctgccc
     5761 gcctccctgg tggagctgcg ccgggtccgc gacctccagc cgtacgtggg caaggtcctc
     5821 gaggccaaga tcatcgagct cgacaagaac cgcaacaacg tggtgctgtc ccgccgggcg
     5881 tggctggagg agacccagcg cgagcagcgc gaggagttcc tggagaacct caagcctggc
     5941 gagcgccgca agggtgtcgt ctcctcggtg gtcaacttcg gtgccttcgt cgacctcggc
     6001 ggcatggatg gcctcgtcca cgtgtccgag ctgtcctgga agcacgtcga ccacccgagc
     6061 tcggtcgtcg ccgtcggtga cgagatcgag gtgcaggtcc tggaagtgga tctggaccgg
     6121 gagcgcatca gcctctcgct caaggccacc cagcaggatc cgtgggagga gttcgcgtcc
     6181 ggtcaccggg tcggcgagct cgtctacggc cgggtcacca agctcgtccc cttcggcgcc
     6241 ttcgtccagg tgggcgacgg catcgagggc ctggtccaca tctcggagat gtcggcccat
     6301 cacgtcgagc tgcccgagca ggtcgtcacg ccgggcgagg agctgtgggt gaaggtcatc
     6361 gacctcgacc tccagcgccg ccgcatcagc ctgtcgatca agcaggccgc ggaaggtggc
     6421 gtcgtggccg ccgagtacca ggagcacttc ggcgagcacg cctacgacga gtccggcaac
     6481 tacgtcggcc cgtccggtga gatcgacgag gcctgggccg agtactacga ggagcacggc
     6541 ctggatccgg ccaccggcca gcccaaggtc ccgaccgcca cgccggcgac cgaggaggac
     6601 gccgccgccg gcgacagcaa cggcgccatg gccgccgccc tggccgatgc cggcctggag
     6661 gcccccaccg agcccgagga cgccgaagcc cccgctgacc ccgaggcgga ggtcgccgag
     6721 gcgcccgccg aacccgacgg ggccgaggcc
//
LOCUS       DASTFV010000060         4399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_198485, whole genome shotgun sequence.
ACCESSION   DASTFV010000060 DASTFV010000000
VERSION     DASTFV010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198485"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFP02_01105"
     CDS             <1..262
                     /locus_tag="VFP02_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9541647.1"
                     /translation="VLVLDEATSSLDPGTEALVEAAVVRLSEGRTVILVAHRLSTAER
                     ADRVAVVSGGRLVEVGPHAELVAKDGQYAAMFATWSGGSTAA"
     gene            291..2369
                     /locus_tag="VFP02_01110"
     CDS             291..2369
                     /locus_tag="VFP02_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET9541648.1"
                     /translation="MGVTRGAGAAAVLMSLLGVALAVGSVGPPGSRSADAASAPNVVV
                     IMTDDQTLAQMVALPETTALLGDAGVTFSDAVVSYPMCCPSRATFLTGQYTQNNGVRQ
                     NTAADDDNPLPREAFPVADVEAWPAFARDAERRSIAVALEDAGYHTGLLGKYMNGYQR
                     RAPVPPGWSDWRAAPSPSYWNTRLNENGVEVEYPGQFQTDVYREVAGDMIGQASDSRQ
                     PFFLMLNFSAPHTAGPGVHTYADRHQSLFPDALAPRSAAYDEADTSDKPAFLRDRYPR
                     LSTAQVQTVDARWRDSLRSLAAVDEAVGAIVEDLADTGELEDTVLIFTSDNGLYHGEH
                     RIPGSKYLPYEPAIRVPLIVAGPAVADGLRGTVSDAAVANVDLAATILDLANVEPLAP
                     VDGRSLLPLLTGEPVPWQAGATPSATHARPIYLMGVGSSIRGNVPQTYSGVRSSDGWS
                     YIRWEDQAGSTELYDLVNDPEQTENLARDPSYRAIRARLERQRHHLVRCAGRSCDVPP
                     YGYLDLPRTGAPPRWFAAARWADAGDVDVAFADGTFGPSRPGPRGRLLPWLWRYAGSP
                     PAAEQVSHFTDVADGLRPALDWAVANGIVGDRSRFAPARPVTRAVWATWLWRLAGRPA
                     PLPGHRPRDVAAGHPAQAAIRWLVEDPDGAGPLRAVGSSYGNGTFGPADAVSRESAVI
                     WLRNLDRRLT"
     gene            complement(2394..>4399)
                     /locus_tag="VFP02_01115"
     CDS             complement(2394..>4399)
                     /locus_tag="VFP02_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9541649.1"
                     /translation="LEEVPVGVFATDGDLRVLWANPRVGDLTGVRPVDAIGRALHELA
                     GPDADRVERALREAWIRQEPCLTWFRAPHSDGGRHRVQLRATVALGADGKPIGLIGSL
                     DDVAAVTHAEDPAARAATILQSSSDAVFSITNDSKVWFLNGAAEDLFGNRGIAQPTLQ
                     ALFTSESLRRFDAEILTRVEREGSWTGELTMRGRGGNPIYVEVTLRAERDEDGHLREL
                     SGLARDMTDRRKVEAAWAHRALHDPLTGLPNRAKLLDHLEKALARASRDGTHVALLFF
                     DVDRFKLVNDTRGHDVGDDLLCQIAERIRTVLRPHDLVARLGGDEFVVLFDGVESDSH
                     ALAVGHRVTEVIETQPMALPSGDYSVTVSAGVALSHTGTLPTRLMREADAAMYRAKEL
                     GRARLELYDDELRRRQAERVALSDDLSRAMADNAITLHYQPFVSLRTGQVTGMEALAR
                     WSHPTRGPLPPDEFIGLAEQTGLIDQLGPVLLRNACTFAVGWQSEVAQAPRLHVNISA
                     RQLADDQFPTTVRHILAESGLAPHRLCLEISESDLQGDTDVAVVALLRLKDIGIDLAI
                     DDFGTGFSSLPHLRSFPVDLLKVDRSFIDGLGPDPEDSAIAAAIVSLAHNLEIEAVAE
                     GVESVEQVSELQALGCDAAQGFLFARPMAAADVIPYLDHVYAV"
BASE COUNT          577 a         1538 c         1652 g          632 t
ORIGIN      
        1 ggtgctggtg ctggacgagg ccacgtccag cctcgaccct ggcaccgagg ccctggtcga
       61 ggccgccgtg gtccgcctct ccgagggtcg gacggtgatc ctggtggccc accgcctgtc
      121 gaccgccgaa cgggccgacc gggtggccgt cgtgtccggc ggccggctgg tcgaggtcgg
      181 ccctcacgcc gagctggtgg ccaaggacgg ccagtacgcc gccatgttcg ccacctggtc
      241 cggcggctcc accgccgcgt agtccgcagc gatgccagta ggttggatcg gtgggcgtga
      301 cgcgcggggc cggcgcggct gcagtcctga tgtcgctcct gggcgtggcc ctcgcggtcg
      361 gctcggtcgg cccaccaggt agccgttcgg ccgatgcggc gtcggccccc aatgtcgtgg
      421 tcatcatgac cgacgatcag acgctcgcac agatggtcgc cctgccggag accaccgccc
      481 tcctgggtga cgctggggtc acgttcagcg acgccgtggt ctcctacccg atgtgctgcc
      541 cgagtcgggc caccttcctg acgggccagt acacgcagaa caacggcgtg cgccagaaca
      601 ccgccgccga cgacgacaac ccgttgcccc gggaggcgtt cccggttgcc gacgtcgagg
      661 cctggcccgc cttcgcccgt gatgcggagc gacgctcgat cgcggtcgcc ctcgaggacg
      721 ccggctatca caccggcttg ctcggcaagt acatgaacgg gtaccagcgg cgcgctccgg
      781 tcccgcccgg ctggtcggac tggcgggcgg cgccgagtcc ctcgtactgg aacacgcgac
      841 tcaacgagaa cggcgtggag gtcgagtacc ccggccagtt ccagaccgac gtctaccgcg
      901 aggttgccgg cgacatgatc ggccaggcct ccgacagccg gcagcccttc ttcctgatgc
      961 tcaacttctc ggcgccccac actgccggac ccggcgtcca cacctacgcc gaccgtcacc
     1021 agtcgctgtt cccggacgcc ctggcccccc gttcggccgc ctacgacgag gccgatacct
     1081 cggacaagcc ggccttcctc cgcgaccggt acccgcggct cagcacggct caggtgcaga
     1141 ccgtggacgc ccgatggcgg gacagcctcc ggtcattggc ggcggtggac gaggccgtcg
     1201 gcgcgatcgt cgaagacctg gccgacaccg gcgagctgga agacacggtg ctgatcttca
     1261 ccagcgacaa cggcctgtac cacggggagc accggatccc ggggagcaag tacctgccct
     1321 acgagccggc gatccgtgtg ccgttgatcg tcgccgggcc cgcggtggcc gacggcctcc
     1381 gggggacggt ctcggacgcc gcggtggcca acgtggacct cgccgcgacc attctcgacc
     1441 tggccaatgt cgagcccctg gcgcccgtgg acggacggtc cctcctgccc ctcctcaccg
     1501 gcgagccggt gccatggcag gcgggggcca cgccctcggc cacccacgcc cgtcccatct
     1561 acctgatggg cgtcggctct tcgatccggg gcaacgtgcc ccagacctac agcggcgtgc
     1621 ggagcagtga cgggtggtcc tacatccgct gggaggacca agcgggcagc accgagttgt
     1681 acgacctcgt gaatgacccg gagcagacgg agaacctggc ccgcgacccc tcgtaccggg
     1741 cgatccgggc ccgactggaa cggcagcggc accacctcgt ccgctgtgct ggccgctcct
     1801 gtgacgtccc gccgtacggc tacctcgacc tgcctcgcac cggtgcgcca ccccgctggt
     1861 tcgccgccgc ccgatgggcc gacgcggggg acgtcgacgt ggcgttcgcc gacggcacct
     1921 tcggcccctc ccgccccggg ccgcggggcc gcctcctgcc ttggctgtgg cgctacgccg
     1981 gctcgccccc ggccgcggaa caggtctccc acttcaccga cgtggccgac ggcctccgac
     2041 cggcgctgga ctgggcggtc gcgaacggca tcgtcgggga tcgatcgagg ttcgccccgg
     2101 cgcgcccggt cacgcgtgcg gtctgggcga cgtggttgtg gcgcctggcc ggccggccgg
     2161 ccccgctccc gggtcaccgc ccacgggacg tggcggcggg acatcctgct caggccgcga
     2221 tccggtggct ggtcgaggac ccggacggcg ccggtccgct ccgggctgtc gggtcgagct
     2281 acgggaacgg cacgttcggc ccggccgatg cggtctcccg cgagtcggcc gtcatctggt
     2341 tgcgcaacct cgaccgtcgg ctgacgtagc cacgagccgt ggcccgccgg gccctacacc
     2401 gcgtagacgt ggtcgaggta ggggatgacg tcggcggcgg ccatggggcg ggcgaagagg
     2461 aagccctggg ccgcgtcgca gccgagggcc tggagctcgg agacctgctc gaccgactcg
     2521 acgccctcgg ccacggcctc gatctcgagg ttgtgggcca ggctgacgat ggcggcggcg
     2581 atggcggagt cctcggggtc cggaccgagg ccgtcgatga aggaccggtc gaccttcagg
     2641 aggtcgaccg ggaagctgcg gaggtggggc agggacgaga agccggtgcc gaagtcgtcg
     2701 atggcgaggt cgatcccgat gtccttcagg cgcaggaggg ccacgacggc gacgtcggtg
     2761 tcgccctgga ggtcggactc ggagatctcc agacagagcc ggtgcggggc gagcccggac
     2821 tcggcgagga tgtggcgcac cgtggtcggg aactggtcgt cggccagctg ccgggcggag
     2881 atgttcacgt ggaggcgggg agcctgggcc acttcggact gccagcccac ggcgaaggtg
     2941 caggcgttgc ggagcaggac cgggccgagc tggtcgatga gaccggtctg ttcggcgagg
     3001 ccgatgaact cgtccggcgg gagggggccc cgggtggggt ggctccagcg ggccagcgcc
     3061 tccatgccgg tcacctggcc ggtgcgcagg ctgacgaagg gctggtagtg gagggtgatg
     3121 gcgttgtcgg ccatggcccg gctgaggtcg tcggagaggg ccacccgttc ggcttgccgg
     3181 cggcggagct cgtcgtcgta gagctcgagg cgggcccggc cgagctcctt ggcccggtac
     3241 atggcggcgt cggcctcccg catgagccgg gtcggcaggg tgcccgtgtg ggagagggcc
     3301 acgccggcgc tcacggtcac cgagtagtcg ccgctgggca gggccatcgg ctgcgtctcg
     3361 atgacctcgg tgacccggtg gccgacggcc agcgcgtggc tgtcgctctc cacgccgtcg
     3421 aagaggacga cgaactcgtc accgccgagg cgggcgacca ggtcgtgggg tcgcaggacc
     3481 gtgcggatgc gctcggcgat ctggcagagc aggtcgtcgc cgacgtcgtg gccgcgggtg
     3541 tcgttgacca gcttgaagcg gtcgacgtcg aagaagagga gggcgacgtg ggtgccgtcc
     3601 cgactggccc gggccagtgc cttttcgagg tggtcgagga gcttcgcccg gttgggcagg
     3661 ccggtgagcg ggtcgtgcag ggcccggtgg gcccaagccg cctcgacctt gcggcggtcg
     3721 gtcatgtccc gggccagccc gctgagctcg cggaggtggc cgtcttcgtc ccgttcggcc
     3781 cgcagggtca cctcgacgta gatcgggttc ccaccccggc cccgcatggt gagctcgccc
     3841 gtccagctcc cctcccgctc gacccgggtc aggatctcgg cgtcgaagcg gcgcagggac
     3901 tccgacgtga acagggcctg gagcgtcggc tgggcgatcc cgcggttgcc gaagaggtcc
     3961 tcggcggcgc cgttgaggaa ccagaccttg ctgtcgttgg tgatgctgaa cacggcgtca
     4021 ctgctcgact gcaggatcgt ggccgcccgg gcggcggggt cctcggcgtg ggtgacggcg
     4081 gccacgtcgt cgagggagcc gatcaggccg atcggcttcc cgtcggcgcc gagggcgacg
     4141 gtggcccgga gctgcacccg gtggcgaccg ccgtcgctgt gcggcgcccg gaaccaggtc
     4201 aggcagggtt cctggcggat ccaggcctcg cgcagcgccc gttcgacccg gtcggcgtcg
     4261 gggccggcga gctcgtggag ggcccgaccg atggcgtcga cggggcgcac gccggtgagg
     4321 tcgccgacgc gcgggttggc ccagaggacc cggaggtccc cgtcggtggc gaacacgcct
     4381 accgggacct cctcgagga
//
LOCUS       DASTFV010000061         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_201835, whole genome shotgun sequence.
ACCESSION   DASTFV010000061 DASTFV010000000
VERSION     DASTFV010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201835"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VFP02_01120"
     CDS             <1..491
                     /locus_tag="VFP02_01120"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET9541650.1"
                     /translation="NELVVAGIVQSVITPFDEVPRNSAIDLVVSLGPRPRTIPADAVG
                     RPFSEVAAELEDLSLVVVREDEPTTDVPEGTVTSIDPAAGAVVERDSTVTVRVAVAQV
                     EVPDVTGLDPVSAADALAAVGLRVSQTVGPPNRNVVDTDPGPGTLVDPNSAVTLFTRR
                     FG"
     gene            650..2140
                     /locus_tag="VFP02_01125"
     CDS             650..2140
                     /locus_tag="VFP02_01125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9541651.1"
                     /translation="MTAERPPDLSVEAPGLSEESSVAWPLLLGQRVTEKAEDKGWYPW
                     VVLATTLFGLLTVTFTITILAVSIPGIAEDLNTSESTLTWVITGPLLAFAVVGPALGK
                     LGDRNGYRRIFLIGMAGSAVFAALSAVAPTAGALILFRVLGAGLGAATGPAAMALINT
                     TFPRERRAQAMGFWTLVMAGGPVLGVVIGGPVVEHFSWRLIFVAQVPVALLGLFVAFI
                     LLPDTEREERAAFDLAGTVLLAAAVAGILLGLNRGPEVGWSSPIVIAGFALAPILLMA
                     FVRVERRAADPLMPLRYLRSRNVGLPLIVEFTNNFAYMGGFILTPLLLEQVLGYGETR
                     AGLLSIARPLAFSISGPLAGFFAVRVGERKAATFGTLAIVCSMIGMSLVSPVSSDGLI
                     VVALALSGVGAGATLPAMAATIANAVDDRDLGVIGAAQQMVAQLGAVTGIQILQTVQA
                     SREAAVGEVDAFGQAFLTGAAVAFVGTLCALSVRSTVRDPVPATMA"
     gene            complement(2235..>2492)
                     /locus_tag="VFP02_01130"
     CDS             complement(2235..>2492)
                     /locus_tag="VFP02_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET9541652.1"
                     /translation="TYLDTSGGEAVMELGELDTPDLTVTTDYATAKAITVDGDAQVAM
                     QAFMQGKVKVQGDMSKLMLLQQAPADPVQTEIQAKIQELTE"
BASE COUNT          299 a          912 c          865 g          416 t
ORIGIN      
        1 acaacgagct ggtcgtggcc gggatcgtgc agtcggtcat cacccccttc gacgaggtgc
       61 cccgcaacag cgccatcgac ctcgtcgtgt ccctcggtcc ccggccccgc accatcccgg
      121 ccgatgccgt cggacggccc ttctccgagg tggccgccga actggaagac ctctccctgg
      181 tggtcgtgcg ggaggacgag ccgaccaccg acgtgcctga gggcacggtc acctccatcg
      241 accccgcagc gggcgcggtc gtcgaacggg actcgacggt gaccgtccgc gtggccgtgg
      301 cccaggtcga ggtccccgac gtgaccggcc tcgaccccgt ctcggctgcc gatgccctcg
      361 ctgcggtcgg gctgagggtc tcccagacgg tgggaccgcc gaaccggaac gtggtcgaca
      421 ccgaccccgg gccgggcacg ctggtcgacc cgaactcggc ggtgacgctc ttcacccgcc
      481 gtttcgggta acccgggcat ccaccacagg gggcggcggc ctccctagcg tttcgcccga
      541 accgggcccg ctggggtggt gccggccgtg acgctcccca gccgccacga cgctgccacc
      601 ccggccggcc cggttcaccg ctgacacgac gctcgttagc ctcccgccgg tgaccgccga
      661 acgcccgccc gacctctcgg tcgaggcccc ggggctcagc gaggagagtt cggtggcgtg
      721 gccgctgctg ctgggccagc gggtcaccga gaaggccgag gacaagggct ggtacccgtg
      781 ggtcgtactg gccacgaccc tgttcgggct gctcacggtc acgttcacca tcacgatcct
      841 ggccgtgtcg atccccggca tcgccgagga cctgaacacc agcgagagca ccctgacctg
      901 ggtcatcacc gggcccctcc tggcctttgc cgtcgtaggc ccggcgctgg gcaagctcgg
      961 cgaccggaac ggctaccgcc gcatcttcct catcggcatg gccggcagtg ccgtcttcgc
     1021 ggcgttgtcc gccgtggccc cgacggcggg ggcgttgatc ctcttccggg tgctcggggc
     1081 cgggctgggg gccgccaccg gcccggccgc gatggccctg atcaacacca cgttcccccg
     1141 cgagcgtcgg gcccaggcca tgggcttctg gaccctcgtc atggcagggg gcccggtgct
     1201 cggggtcgtc atcggcgggc cggtcgtgga gcacttcagc tggcgactga tcttcgtggc
     1261 gcaggtcccg gtcgccctcc tgggcctctt cgtcgccttc atcctcctgc ccgacaccga
     1321 gcgggaggag cgcgccgctt tcgacctggc gggcaccgtc ctgctggccg cggcggtggc
     1381 tgggatcctg ctcggcctga accgcggccc ggaggtgggc tggagcagtc ccatcgtcat
     1441 cgccggcttc gcgctggccc cgatcctgct gatggcgttc gtccgggtgg agcggcgggc
     1501 cgccgacccc ctgatgcccc tgcgctacct ccgcagccgc aacgtgggcc tgcccctgat
     1561 cgtggagttc accaacaact tcgcctacat gggtggcttc atcctgacgc cgctcctgtt
     1621 ggaacaggtc ctgggctacg gcgagacccg ggccgggctg ctctcgatcg ctcgtcccct
     1681 cgccttctcg atctcggggc cgttggccgg cttcttcgcc gttcgggtgg gcgagcgcaa
     1741 ggcggccacg ttcgggacgc tggcgatcgt ctgctcgatg atcgggatgt ccctggtctc
     1801 accggtcagc tcggacgggt tgatcgtggt cgcgctcgcc ctctcgggcg tcggcgccgg
     1861 ggccaccctg ccggccatgg cggcgaccat cgccaacgcc gtcgacgacc gggacctcgg
     1921 cgtgatcggg gcggcgcagc agatggtcgc ccagctcggt gccgtcaccg gcatccagat
     1981 cctgcagacc gtgcaggcca gccgcgaggc cgcggtcggc gaggtcgacg ccttcggcca
     2041 ggccttcctc accggtgccg ccgtcgcctt cgtcggcacc ctgtgcgccc tctcggtgcg
     2101 ttcgaccgtc cgcgatcccg ttcccgcgac gatggcctag ccagaagggt cgctgcggag
     2161 ttggagcgga gcagctcgca gaccggcagc ttcggcggcg ccggacttgc tgagcgaagc
     2221 gaagccagtc agagctattc ggtgagctcc tggatcttgg cctggatctc ggtctggacc
     2281 ggatcggccg gggcctgctg gaggagcatg agcttgctca tgtcgccctg gaccttgacc
     2341 ttgccctgca tgaaggcctg catggcgacc tgggcgtccc cgtccacggt gatggccttg
     2401 gcggtggcgt agtcggtggt cacggtgagg tccggggtgt ccagctcgcc cagctccatg
     2461 acggcctcgc cgcccgaggt gtcgagatag gt
//
LOCUS       DASTFV010000062         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_203143, whole genome shotgun sequence.
ACCESSION   DASTFV010000062 DASTFV010000000
VERSION     DASTFV010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203143"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFP02_01135"
     CDS             <1..248
                     /locus_tag="VFP02_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541653.1"
                     /translation="EIWRGHLNEAAAWAGETAPRGEIALVVQGALPPGPADEADVEAA
                     VQARLDAGDSARDAATTVAADLGVPKRQAYAVANRLA"
     gene            298..1407
                     /locus_tag="VFP02_01140"
     CDS             298..1407
                     /locus_tag="VFP02_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET9541654.1"
                     /translation="MVIAVLCALVVGGVIAFAVLKLLPATVLDLVTRHVDAQAQAGAR
                     ELDLRQGAITEQMDLRQGAISEQVQAANTELRELRELVATLRRDGAAQQGELATRLEE
                     AVATNRQVAELTGSLREALASPKARGTWGERTAEDILRVAGFIEGVNYRKQRSVQGGS
                     TIPDFTFLLPHDRVVHMDVKFPIAGYLRHLDASSDLERQQAAKQFVRDVRQRLKELAG
                     RHYIDPTCTVDYLLVFIPNESVYAFIHEHDPELVDFALQQKAVLCSPTTLFPVLAVIR
                     QAMDNFMVDQTSEQILRCLGAFTDQWEKLAEAIDKVGKQIETTQKAFGELNGPRRRAV
                     ERTFAQVDELRTNNQLPLAAVDVGEVPGLSDRRAG"
     gene            1458..2963
                     /gene="metG"
                     /locus_tag="VFP02_01145"
     CDS             1458..2963
                     /gene="metG"
                     /locus_tag="VFP02_01145"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET9541655.1"
                     /translation="MSRYYVTTPIYYVNDAPHIGHAYTTVTADALARWHRLLGEDVFF
                     LTGTDEHGLKVARAAEEHGLTPQEQADRTSERYKETWALLNISHDDFIRTTEPRHHRA
                     VQELLTAAKDNGHIYKDTYEGLYCVACEAYYTEDDLVDGNCPIHGRPVEFLSEENWFF
                     ALSAFERPLLDWYEANPAAVQPEGKRNEALGLIRQGLRDISISRSSIDWGVQVPWDPD
                     HVFYVWYDALINYATAVGYGRDPDRFADWWPEVHHVIGKDILRFHCVYWPALLLAAGL
                     APPKRISVHGFLLVGGEKMSKTSLNQIVPADLVEDFGVDGYRYHFLGATPFGPDGDFS
                     YEGMVERYNSDLANNLGNLQSRVATVVGKKCDAVGPAPRPDSPLAAVANKAYAETAEA
                     WARVQPSLALEATWRLIRETNAYLETNEPWKAEAGAEVDAVLGDALEALRIVTILASP
                     AVPDTCAEIWRRLGLEGRPEAQRLPEAAAWGGYPGGLTVEKGEPLFPRLSG"
     gene            2968..>3178
                     /locus_tag="VFP02_01150"
     CDS             2968..>3178
                     /locus_tag="VFP02_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET9541656.1"
                     /translation="MRWTDDHCHLGHLDLTDDAGVVAALATAAEHGVARVVDVGTDVA
                     DSTAAADRAAAHPAVWATAGVHPHEA"
BASE COUNT          496 a         1189 c         1057 g          436 t
ORIGIN      
        1 aggagatctg gcggggtcac ctgaacgagg ccgcggcctg ggcaggcgag acggcacctc
       61 ggggcgagat cgccctggtc gtgcagggcg cgctgccccc cggcccggcg gacgaggcgg
      121 atgtcgaagc cgccgtccag gcccgtctcg acgccggtga ctccgcccgg gacgccgcca
      181 ccaccgtcgc cgccgatctc ggagtcccga agcgccaggc ctacgccgta gccaatcgcc
      241 tggcctgaga ccggccgggt ccccgttctt gtcacccccc ctgggcagca tgggggcatg
      301 gtcatcgccg tcctctgtgc cctcgtcgtg ggtggggtca tcgccttcgc cgtcctcaag
      361 ctgctgccgg ccacggtcct cgacctcgtc acccgccacg tcgacgccca ggcccaggcc
      421 ggcgcccggg agctggacct gcgccagggg gccatcaccg agcagatgga cctgcgccag
      481 ggggcgatca gcgagcaggt ccaggcggcc aacaccgagc tccgggagct gcgggagctg
      541 gtggccacgt tgcgccgtga cggggccgcc cagcagggcg agttggccac ccgcctcgag
      601 gaggccgtcg ccaccaaccg ccaggtcgcc gagctgaccg gcagcctgcg ggaggccctg
      661 gccagcccca aggcccgggg cacctggggc gagcgcacgg ccgaggacat cctgcgggtg
      721 gccgggttca tcgagggcgt caactaccgc aagcagcggt cggtgcaggg agggagcacc
      781 atccccgact tcaccttcct gctgccccac gaccgggtcg tccacatgga cgtcaagttc
      841 ccgatcgcgg ggtacctgcg ccacctcgac gcctcctcgg acctggagcg ccagcaggcg
      901 gccaagcagt tcgtgcgcga cgtccgccag cggctgaagg agctggccgg ccgccattac
      961 atcgatccga cctgcacggt ggactacctg ctggtcttca tccccaacga gagcgtctac
     1021 gccttcatcc acgagcacga ccccgagctg gtggacttcg ccctgcagca gaaggccgtc
     1081 ctctgctcac cgaccacgct gttcccggtc ctggccgtca tccgtcaggc catggacaac
     1141 ttcatggtcg accagacctc cgagcagatc ctgcgctgcc tcggcgcctt caccgaccag
     1201 tgggagaagc tggccgaggc catcgacaag gtcggcaagc agatcgagac cacccagaag
     1261 gcgttcggcg agctcaacgg cccccggcgc cgggccgtcg agcgcacctt cgcccaggtc
     1321 gacgagctcc ggaccaacaa ccagctgccg ctggcggccg tcgacgtggg tgaggtcccg
     1381 ggcctgtccg accgtcgggc ggggtgagcg gcggtaaccc cggaaggggg gttgcggacc
     1441 gtcggtagcc tcgcgttgtg agccgctact acgtcacgac gccgatctac tacgtcaacg
     1501 acgcgcccca catcggccac gcctacacga cggtcaccgc cgacgccctg gcccggtggc
     1561 accggctgct cggggaggac gtcttcttcc tcacgggcac cgacgagcac ggcctgaagg
     1621 tggcccgggc cgctgaggag cacggcctca ccccccagga gcaggccgac cgcaccagcg
     1681 agcggtacaa ggagacctgg gccttgctga acatcagcca tgacgacttc atccgcacca
     1741 ccgagccccg ccaccaccgg gccgtccagg agctcctgac cgcggccaag gacaacggcc
     1801 acatctacaa ggacacctac gagggcctct actgcgtggc ctgcgaggcc tattacaccg
     1861 aggacgacct ggtcgacggg aactgcccga tccacggccg gccagtcgag ttcctgagcg
     1921 aggagaactg gttcttcgcc ctctccgcct tcgagcggcc actgctggac tggtacgagg
     1981 cgaacccggc ggccgtgcag cccgaaggca aacgcaacga ggccctcggc ctcatccgcc
     2041 agggcctgcg ggacatctcg atcagccgct cgtcgatcga ctggggcgtc caggtcccgt
     2101 gggatcccga tcacgtcttc tacgtctggt acgacgccct catcaactac gccacggcgg
     2161 tcggctacgg ccgcgacccc gaccggttcg ccgactggtg gccggaggtc caccacgtga
     2221 tcggcaagga catcctccgc ttccactgcg tctactggcc cgccctgctc ctggcggccg
     2281 gcctggcgcc gccgaagcgc atcagcgtcc acggcttcct gctggtcggg ggcgagaaga
     2341 tgtccaagac cagcctgaac cagatcgtcc cggcggacct ggtcgaggac ttcggtgtcg
     2401 acggctaccg ctaccacttc ctgggtgcca cccccttcgg cccggacggc gacttcagct
     2461 atgagggcat ggtcgagcgc tacaacagcg acctggccaa caaccttggc aatctccagt
     2521 cccgggtggc gacggtcgtg ggcaagaagt gtgacgccgt cggccctgcg ccgcgcccgg
     2581 acagcccgtt ggccgcggtg gccaacaagg cgtatgcgga aaccgccgag gcctgggccc
     2641 gggtacagcc gtcgctcgcc ctcgaggcca cctggcgtct catccgggag accaacgcct
     2701 atctcgagac caacgagcca tggaaggcgg aggccggggc cgaggtcgac gccgtgctcg
     2761 gcgacgccct cgaggccctg cgcatcgtga cgatcctggc ctcgcccgca gtgcccgaca
     2821 cctgtgcgga gatctggcgc cgcttgggcc tcgagggtcg gcccgaggcc cagcgcctgc
     2881 ccgaggccgc ggcctggggc ggttaccccg gcggcctcac ggtcgagaag ggtgagcccc
     2941 tcttcccccg cctgtcgggc tgatcgggtg cgttggaccg acgatcactg ccacctcggt
     3001 catctcgacc tgaccgatga cgccggcgtg gtcgccgcgc tcgccaccgc cgccgaacac
     3061 ggggtggccc gggtcgtcga cgtgggcacc gacgtggccg actcgacggc agccgccgac
     3121 cgtgccgcgg cccaccccgc ggtctgggcc acggccggtg tccaccccca cgaagccc
//
LOCUS       DASTFV010000063         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_205263, whole genome shotgun sequence.
ACCESSION   DASTFV010000063 DASTFV010000000
VERSION     DASTFV010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205263"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..934
                     /locus_tag="VFP02_01155"
     CDS             158..934
                     /locus_tag="VFP02_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HET9541657.1"
                     /translation="MSTDPTDPVDDEKGPAFVVPPGPTVRFRFTKQGKVRFTSHRDVA
                     RLWERALRRAGVGVVYSEGFSPRPRVAFGLALPTGHSSDGEYVDLRLDHEPTEGLPVL
                     CARLTDVLPDGITVPAAEVISPGTPSLQEAVGACTWTAVVGGPTFTQAKAAVVATLAH
                     TELPIERERKGKAQVVDLRPAIKELTVAEEGADQALTLTMVLATRPASFRPEEVVEAL
                     DPSWRLLRACRTNQWIIDEDTWREPVPLVVPSPRAEVRAS"
     gene            950..2719
                     /locus_tag="VFP02_01160"
     CDS             950..2719
                     /locus_tag="VFP02_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HET9541658.1"
                     /translation="MPDDRDADGTQERDESAGDTSRLADELPELPDRISENRPSAEAA
                     DRATVRRPQIGDTRPAPPSDDGDGAPTSGRGDGGGNGETSSSARRRRRRGGRGRGSGG
                     QGGGGGQGGGGRGAGNRSQSGGGNRDGGRQKVIPVEAMMVDDEDALELDEKSLKRRRG
                     RRRKGRAVGRYSMAVNVTAGVTQIAVLEGRSLVEHQVSRPSDDVSQIHGNIYTGRVQN
                     VLPGMEAAFIDIATPKNAVLYRGDVQYDPEDIEEKGQARIEQILKNGQTIICQVTKNP
                     IGAKGARLTQEVSLPGRFVVLVPGTSTYGISKRLPDNERKRLRGILDKVKLDGHGVIV
                     RTAAENVTEEEITRDLSRLVEQWRQIEALAKSSKAPALLYREPDMALRVIREEFNPDY
                     RSVTIDDPALADQVRDYVDSVSPGLGERVDYYDPEAEGLPLFERLHIHEQIHKALDKK
                     VWLPSGGSLIIEHTEALTVIDVNTGKNVGKSSLEETVYVNNLEAAEEIARQLRLRDIG
                     GIIVIDFIDMEVKANRAEVGKTFREALSRDKTRTQVFDISELGLVEMTRKRIGEGLLE
                     SLSSTCPTCTGRGRIVDSELMGE"
     gene            2753..>2946
                     /locus_tag="VFP02_01165"
     CDS             2753..>2946
                     /locus_tag="VFP02_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL21 family ribosomal protein"
                     /protein_id="HET9541659.1"
                     /translation="MYAVISTGGKQYRVTEGERLEVERLGDPDESVELSPILVVDGDR
                     VVSSPKDLAKAKVKATIVAEA"
BASE COUNT          484 a         1053 c         1024 g          385 t
ORIGIN      
        1 cctgctacga ctgcggggcc tgcaccggct acggcatcga gcacatcgtc gcctcggcca
       61 agccgccggc cgggggcagc cagggcaccg gtcagggcct ggagtggcac agcgccgtgc
      121 ccgtcgagct cgcggccaag ccggtcggcg tgggctcgtg agcaccgacc cgaccgaccc
      181 ggtcgacgac gagaaggggc ccgccttcgt cgtcccgccc ggtccgaccg tccgcttccg
      241 cttcaccaag cagggcaagg tccgcttcac cagccaccgc gacgtggccc gcttgtggga
      301 gcgggcgctg cgccgcgccg gcgtcggcgt ggtctacagc gagggcttct cgccccgacc
      361 tcgggtcgcc ttcgggctgg ccctgcccac cggccactcc tccgacggtg agtacgtcga
      421 cctccgcctc gaccatgagc ccaccgaagg cctacctgtt ctctgtgccc gcctgaccga
      481 tgtcctgccg gacgggatca ccgtcccggc cgcggaggtc atctccccgg gcaccccctc
      541 cctgcaggaa gccgttggcg cctgcacctg gaccgccgtg gtcggcggtc cgaccttcac
      601 ccaggccaag gccgccgtcg tggcgacctt ggcccacacg gagctcccca tcgagcggga
      661 gcgcaagggc aaggcccagg tcgtcgacct gcggcccgcc atcaaggaac tgaccgtggc
      721 ggaggaaggg gccgatcagg ccctcaccct gaccatggtc ctggccaccc gtccggcatc
      781 gttccgcccc gaggaagtgg tcgaggccct cgacccctcg tggcgcctgc tgcgggcgtg
      841 ccgaaccaac caatggatca tcgacgagga cacctggcga gaacccgtgc cactggtcgt
      901 cccgtcgccg cgcgccgagg tgcgcgcgtc atgagagagg acgtacccca tgcccgacga
      961 ccgcgatgcg gacgggaccc aggagcgaga cgagtcggcg ggggacacca gccgactcgc
     1021 tgacgagctc cccgagctgc cggaccggat ctccgagaac cgcccctccg ccgaggcggc
     1081 ggaccgggcc acggtccgcc ggcctcagat cggtgacacg cgcccggcgc cgccgtccga
     1141 cgacggtgac ggtgcgccga cgtccggccg cggcgacggc ggtggcaacg gtgagacctc
     1201 ctcgagcgcc cggcgccgcc ggcgccgcgg cggtcgcggt cgcggcagtg gcggccaagg
     1261 gggcggcggt ggccaggggg gcggcggccg cggtgccggg aaccgcagcc agagcggggg
     1321 tggcaaccgg gacggtggcc gccagaaggt gatcccggtc gaggccatga tggtcgacga
     1381 cgaggacgcc ctggagctgg acgagaagtc gctgaagcgt cgccggggcc gtcggcgcaa
     1441 gggccgggcc gtcgggcgct actcgatggc ggtcaacgtc acggccggtg tgacccagat
     1501 cgccgtgctg gagggccgct ccctggtcga gcaccaggtg tcccgcccgt ccgacgacgt
     1561 cagccagatc cacgggaaca tctacaccgg tcgggtccag aacgtcctgc ccggcatgga
     1621 ggcggcgttc atagacatcg ccacccccaa gaacgcggtc ctctaccggg gcgatgtgca
     1681 gtatgacccc gaggacatcg aggagaaggg ccaggcccgg atcgagcaga tcctgaagaa
     1741 cggccagacg atcatctgcc aggtcaccaa gaaccccatc ggggccaagg gcgcccgcct
     1801 gacccaggag gtctcgctgc ccgggcgctt cgtggtcctg gtgccgggca cgtccaccta
     1861 cggcatctcc aagcgcctgc cggacaacga gcgcaagcgc ctgcggggga tcctggacaa
     1921 ggtcaagctg gacggtcacg gtgtgatcgt gcggacggcc gccgagaacg tcaccgagga
     1981 ggagatcacc cgggacctga gccggctggt cgaacagtgg cgccagatcg aggcgctggc
     2041 caagagctcg aaggcgccgg ccctgctcta ccgcgagccg gacatggccc tgcgggtcat
     2101 ccgcgaggag ttcaaccccg actaccggtc cgtcaccatc gacgatccgg cgttggccga
     2161 tcaggtccgg gactacgtgg acagcgtttc gcccggtctc ggcgagcggg tcgactacta
     2221 cgaccccgag gccgagggcc tgcccctctt cgaacgcctc cacatccacg agcagatcca
     2281 caaggccctc gacaagaagg tctggttgcc gtccgggggc tcgctcatca tcgagcacac
     2341 cgaggccctc acggtcatcg atgtgaacac gggcaagaac gtgggcaagt cgagcctcga
     2401 ggagaccgtc tacgtcaaca acctggaggc ggccgaggag atcgcccgcc agctgcggct
     2461 gcgcgacatc ggcgggatca tcgtcatcga cttcatcgac atggaggtca aggccaaccg
     2521 ggccgaggtg ggcaagacgt tccgcgaggc gctgtcccgg gacaagaccc ggacccaggt
     2581 cttcgacatc tccgagctgg gcctggtcga gatgacccgg aagcgcatcg gtgagggtct
     2641 ccttgaatcc ctgtcctcga cctgccccac ctgcaccggt cgaggccgga tcgtggacag
     2701 cgagctcatg ggcgagtgag cggcggcgcg ccgccggtag gcttctccct ccatgtatgc
     2761 agtcatcagc accggcggta agcagtaccg cgtcaccgag ggcgagcgcc tcgaggtcga
     2821 gcgcctcggc gaccctgacg agtccgtcga gctctcgccg atcctggtcg tcgacggcga
     2881 ccgagtcgtc tcgtcgccca aggacctggc caaggccaag gtgaaggcca ccatcgtggc
     2941 cgaggc
//
LOCUS       DASTFV010000064         3172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_205536, whole genome shotgun sequence.
ACCESSION   DASTFV010000064 DASTFV010000000
VERSION     DASTFV010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205536"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="VFP02_01170"
     CDS             complement(<1..1313)
                     /locus_tag="VFP02_01170"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HET9541660.1"
                     /translation="MAETMGTVTEAPTGLLSGYDTGGFYDEAVDETDELRPEYEVLFD
                     RLRTMTHEELARRAAMSDDTMRKRGVTFTLSADAEAEAEGDEDLERTLPMDVLPRVIG
                     GAAWRDLEAGLIQRVSALNAFLEDLYVGERAAVRDGIVPWWLVTSSDGFVREAMGVPA
                     PSGVRCLVAGLDLVRGADGRLRVLEDNLRNPSGISYVLENRDLMTRLFPSIVRAHRVR
                     PVGNYGERLLRALRSVAPSGVADPCVVVMTPGIHNAAYFEHAFLAHQMGVELVEGHDL
                     LVDGQRVFMRTTEGPRPVHVIYRRLDDRFLDPVGFRSGSYIGVPGLLSAARAGAVTMA
                     NAVGNGVADDKAVYPFVPDLVRYYLNEEPLLPNVGTYCLWDPDQRAAVLDRLDELVTK
                     PVDGAGGYGLVIGPVASDEQLAVLRAQIEADPRGYISQEVVQLSRV"
     gene            complement(1369..2211)
                     /locus_tag="VFP02_01175"
     CDS             complement(1369..2211)
                     /locus_tag="VFP02_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9541661.1"
                     /translation="MTIDEAPEPIPIIPDVPRALSPLVRRICADNPGVMTGPGTNTYL
                     IGVDEIAVIDPGPADEAHLDAVAGCGGDRIRWILLTHTHPDHGPGAIGLKERTGAKIM
                     AFESRDGIDVDGTLADGDMVEGTEWRLTALHTPGHAGNHLCFMLEEERCLFTGDHVMD
                     GSTVVIKPPDGDMAAYLASLERIKAMRLSVIAPAHGHPIEDPKARIDWYIKHRIEREA
                     AIAAALKEAGTASIDDLVAAVYTDVDPERHPIARHSTYAHLLKLRDEGTVKGDDLDGK
                     WTTA"
     gene            complement(2208..2957)
                     /locus_tag="VFP02_01180"
     CDS             complement(2208..2957)
                     /locus_tag="VFP02_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541662.1"
                     /translation="MTVLIPRPAATTMVLRDGTEGLEVLLLRRSPHTVFVPGAHVFPG
                     GAVEAADHDPHPVAAGAPAEPDASARLGLDTGGLAFWLAAVRETFEEAGLLFADGPVD
                     RAAPERAAVDRGERPLAEVCAELGLTLRLGDLRYFGHWITPEGGPRRYTTRFFVAPAP
                     AGQVPIHDDGEAVDNEWVRPRVALDRFTAGDWELITPTERSLHALDRFADVAAVLAHL
                     DTHPPLTDDHGGRRIALPEELATAASQETLT"
     gene            complement(2954..>3172)
                     /locus_tag="VFP02_01185"
     CDS             complement(2954..>3172)
                     /locus_tag="VFP02_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541663.1"
                     /translation="DIVGILLLLPPTRAIARTFLAHVLRGRLKVATAGVAAPGSGGSS
                     WSGSVVDADARERTNRDDGPRGPIPLGP"
BASE COUNT          433 a         1074 c         1223 g          442 t
ORIGIN      
        1 acccgcgaca gctggaccac ctcctgggag atgtagcccc gggggtcagc ctcgatctgg
       61 gcccgcagga cggcgagctg ctcatcgctg gccacggggc cgatgaccag gccatagccg
      121 ccggccccgt cgaccggctt ggtcaccagc tcgtcgagcc ggtccagcac ggcagcccgc
      181 tggtcggggt cccagaggca gtaggtgccg acgttgggga gcaacggctc ctcgttgagg
      241 tagtagcgga cgaggtcggg gacgaaggga tagacggctt tgtcgtcggc caccccgttg
      301 cccaccgcgt tggccatggt gaccgccccc gcccgggcgg cgctgagcag gccgggcaca
      361 ccgatgtagc tgccgctgcg gaagccgacg ggatcgagga agcggtcgtc gaggcgccgg
      421 tagatgacgt gcaccgggcg aggcccctcg gtggtgcgca tgaacacccg ctgaccgtcg
      481 accagcaggt cgtggccctc gaccagctcg acgcccatct gatgggccag gaaggcgtgc
      541 tcgaagtagg ccgcgttgtg gatgccgggg gtcatgacca caacgcaggg atcggccacg
      601 ccggacgggg ccacgctgcg cagggcccgt agcaggcgct cgccgtagtt gcccaccggc
      661 cggacgcggt gcgcccgcac gatcgacggg aacagacggg tcatcaggtc ccggttctcc
      721 agcacgtagg agatgccgct ggggttgcgc aggttgtcct ccagcacccg cagccggccg
      781 tcggcccccc ggaccaggtc gaggccggcg accaggcaac gcacgcccga cggggcgggc
      841 acgcccatgg cctcccggac gaagccgtcc gaggacgtga ccagccacca cggcacgatc
      901 ccgtcccgca cggcggcccg ctcgccgacg tacaggtcct cgaggaaggc gttgagggcc
      961 gacacccgct ggatgagccc ggcctccagg tcccgccacg ccgccccgcc gatcacccgg
     1021 ggcaggacgt ccatgggcag ggtgcgctcg aggtcctcgt cgccctcggc ctcggcctcg
     1081 gcgtcggcgc tgagggtgaa cgtgacgccc cgcttgcgca tggtgtcgtc gctcatggcc
     1141 gcccgccggg ccagctcctc gtgcgtcatc gtccgcaggc ggtcgaagag gacctcgtac
     1201 tcggggcgga gctcgtccgt ctcgtcgacg gcctcgtcgt agaagccccc ggtgtcgtaa
     1261 ccggacagca gtcccgtcgg cgcttccgtc acggtcccca tggtctcggc cacggtggca
     1321 tcagggtgtg gcaaccgtgt ttcggccatg tgaaagcgtc ctacctcttc aggcggtcgt
     1381 ccacttcccg tcgaggtcgt cgcccttgac cgtgccctcg tcccggagct tgagcaggtg
     1441 ggcgtaggtg ctgtggcggg cgatcgggtg ccgctcgggg tccacgtcgg tgtagacggc
     1501 ggcgacgagg tcgtcgatgg acgccgtgcc cgcctccttc agcgcggcgg cgatggccgc
     1561 ctcccgctcg atgcggtgct tgatgtacca gtcgatgcgg gccttcgggt cctcgatggg
     1621 gtgaccgtgg gcgggggcga tgaccgacag gcgcatggcc ttgatccgct ccagggaggc
     1681 gaggtacgcc gccatgtcgc cgtccggggg cttgatgacg acggtcgagc cgtccatcac
     1741 gtggtcaccg gtgaagaggc agcgctcctc ctccagcatg aaacagaggt ggttgccggc
     1801 gtggcccggc gtgtgcaggg cggtcaggcg ccactcggtg ccctcgacca tgtcgccgtc
     1861 ggcgagcgtg ccgtccacgt cgatgccgtc gcgggactcg aaggccatga tcttggcgcc
     1921 ggtgcgctct ttgaggccga tggcacccgg cccgtggtcg ggatgggtgt gggtgagcag
     1981 gatccaacgg atccggtccc caccgcagcc ggccacggcg tcgaggtggg cctcgtcggc
     2041 cggaccaggg tcgatgacgg cgatctcgtc cacgccgatc aggtaggtgt tggtgcccgg
     2101 tccggtcatg acaccggggt tgtcggcgca gatccgccgt accagcgggg acagggcccg
     2161 gggcacgtcc gggatgatcg ggatcggctc gggggcttcg tcgatggtca cgtcagggtc
     2221 tcctgggagg cggcggtcgc gagctcctca gggagggcga tccgtcggcc accgtggtcg
     2281 tcggtcaaag gggggtgggt gtcgaggtgg gccaggacgg cggccacgtc ggcgaaccgg
     2341 tcgagggcgt gcaggctgcg ctcggtcggc gtgatgagct cccagtcccc ggcggtgaag
     2401 cggtcgaggg cgacgcgggg gcggacccac tcgttgtcca cggcctcgcc gtcgtcgtgg
     2461 atcgggacct ggccggcggg ggcgggagcc acgaagaagc gggtcgtgta ccggcggggc
     2521 ccaccctccg gggtgatcca gtggccgaag tagcggaggt cgccgaggcg cagggtcaag
     2581 cccagctcgg cgcagacctc ggccagcgga cgctcgcccc ggtcgacggc tgcccgctca
     2641 ggcgccgccc ggtcgacggg gccgtccgcg aagagcaggc cggcctcctc gaaggtctcc
     2701 cgcacggcag ccagccagaa cgccaagccg ccggtgtcca ggccgaggcg agccgatgcg
     2761 tcgggctcgg caggggcgcc ggccgccacg gggtgcgggt cgtggtcggc cgcctcgacc
     2821 gcgccaccgg ggaagacgtg ggcgccgggc acgaagaccg tgtgcgggct gcggcgcagg
     2881 agcaggacct cgaggccctc ggtgccgtcg cgcaggacca tggtcgtggc cgcggggcgg
     2941 gggatgagga cggtcatggg cccagcggga tcgggccgcg ggggccgtcg tcccggttcg
     3001 tgcgctcgcg ggcgtcggcg tcgaccaccg atcccgacca ggacgacccg ccgctccccg
     3061 gagccgcgac gccggccgtg gccaccttca ggcgaccccg caggacatgg gccaggaagg
     3121 tgcgggcgat ggcccgggtc gggggcaaca ggaggaggat gccgacgatg tc
//
LOCUS       DASTFV010000065         3328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_206173, whole genome shotgun sequence.
ACCESSION   DASTFV010000065 DASTFV010000000
VERSION     DASTFV010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206173"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..617
                     /locus_tag="VFP02_01190"
     CDS             177..617
                     /locus_tag="VFP02_01190"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET9541664.1"
                     /translation="MATLLLLSGPNLNLLGDREPEIYGTETLADHVTAARAAAAERGF
                     ELEHLQSNHEGDLIDEIQGARGRCAGIVINPGAFTHYAWAIHDALAAFDGPIVELHLS
                     DPEAREPWRHLSVVAPVAAATVKGLGAAGYPKAITVVADLLEDP"
     gene            653..1741
                     /locus_tag="VFP02_01195"
     CDS             653..1741
                     /locus_tag="VFP02_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET9541665.1"
                     /translation="MDFRSRFDRLRAELDGAGCDALLVTRLVNIRYLTGFTGSAALLL
                     VKPDRALFVSDGRYRDQSAEQIGAAGVDVDMEILNIGQKEVVVAGAAGVARLGLEADH
                     VTWAQQRKYATDWFPDTELVATEAVVDQLRLVKDAGEVARIEAACATADAALASIRHR
                     LVEGPTEEEFGLELDTAIRRLGAEGNSFETIVASGPNAAKPHAEPGSRRIQEGDLVVL
                     DFGALVDGYCSDMTRTFAIGEISPTQQKMLDVVGASQAAGVEAVAAGVEAKAVDLASR
                     EIIDEAGWGDAFLHSTGHGVGLEIHENPRVAQTSEDTLRSDYIVTVEPGVYLPEHGGV
                     RIEDTVVVTDEGCRVLTRAPKSTDPLAA"
     gene            1772..2335
                     /gene="efp"
                     /locus_tag="VFP02_01200"
     CDS             1772..2335
                     /gene="efp"
                     /locus_tag="VFP02_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET9541666.1"
                     /translation="MPAITTNDLKTGMHLEVEGNLVQVVDFQHVKPGKGHAFVRTTLK
                     NARNGAVVEKTFRAGEKVERVTVDKREMQYLYNDGTDYVFMDNATYDQINVSAKTLGD
                     AANYLVDGSTVILQMFGSEIVGVDLPAAVELAITECEPGIQGDRVSGARKPATLATGL
                     IVQVPLFIEPGERIRVDTRSGEYLSRA"
     gene            2340..2765
                     /gene="nusB"
                     /locus_tag="VFP02_01205"
     CDS             2340..2765
                     /gene="nusB"
                     /locus_tag="VFP02_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET9541667.1"
                     /translation="MSTERRQARERALGLLYEAETKSQSPAALVAEQVLEPDPFTTDV
                     VTGVESEGEEIDALITRFATNWPLNRMPALDRTLLRMGVYELTHRPDVPTGVVLSEIV
                     DLASEYSTDDSGPFVNGVLAAIAAEVRTDGDPHPRSAAG"
     gene            2731..3234
                     /locus_tag="VFP02_01210"
     CDS             2731..3234
                     /locus_tag="VFP02_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9541668.1"
                     /translation="MEIPIPDPPLAEGVVALRPWRLEDAPVLAKAWADPAIRRWCSVP
                     EDATVDGAERWISGSDRCRSDGLALDLAVECDGQLAGEVGLGPIQWAHRRAAVGFWVD
                     ADHRRHNLAAKALTLLAAWALVELPLDTLAGEASSENPASGWTLQAAEFQLVTERNTR
                     QAWIRTA"
BASE COUNT          506 a         1225 c         1136 g          461 t
ORIGIN      
        1 ctgcccacga cggtcgacgg cggcctggac cccggtgcgc tggtcgacct catgcgccgc
       61 gacaagaagg cgttcgacgg cctgaccttc gtgctcgacg gcccccgggg ccctgaggtc
      121 gtcaccgggg tcgaggaccg ccacgtcttg tcggccctgg agcaggtccg cgcctgatgg
      181 cgaccctcct gctcctgtcg ggcccgaacc tgaacctgct gggcgaccgg gagccggaga
      241 tctacggcac cgagacgttg gccgaccacg tcaccgccgc ccgggccgcc gccgccgagc
      301 gggggttcga gttggaacac ctgcagtcca accacgaggg tgacctgatc gacgagatcc
      361 agggggcccg ggggcgctgc gccggcatcg tcatcaaccc cggcgccttc acccactacg
      421 cgtgggccat ccatgacgct ctcgccgcat tcgacgggcc gatcgtcgag ctgcacctgt
      481 ccgatcccga ggcccgcgag ccctggcgtc acctgtcggt ggtcgctccg gtcgcggcgg
      541 cgaccgtcaa gggcctcggg gccgccggct atccgaaggc gatcaccgtg gtcgccgacc
      601 tcctggagga cccatgaacc ccgccattgc gcttgccaac gagctccccg ccatggactt
      661 ccggtctcgc ttcgaccgct tgcgggcgga gttggacggt gccgggtgcg acgccctgct
      721 cgtcacccgg ctggtcaaca tccgctacct gaccgggttc accgggtcgg cggccctctt
      781 gctggtcaag cccgaccggg cgttgttcgt cagcgacggc cgctaccggg accagtcggc
      841 cgagcagatc ggcgccgccg gggtcgacgt ggacatggag atcctgaaca tcggccagaa
      901 ggaggtcgtc gtcgccggcg ccgccggcgt ggcccgcctc ggcctggagg ccgatcacgt
      961 gacctgggcc cagcagcgca agtacgccac cgactggttc cccgacaccg agctggtggc
     1021 caccgaggcc gtcgtcgacc agcttcgcct ggtcaaggac gccggagagg tggcccgcat
     1081 cgaggccgcc tgcgccaccg ccgacgccgc cctcgcgtcg atccgccacc ggctggtcga
     1141 agggccgacc gaggaggagt tcggcctgga gctggacacg gccatccgtc gcctcggggc
     1201 cgaaggcaac agcttcgaga cgatcgtcgc ctccgggccc aacgcggcca agccccacgc
     1261 cgagcccggg tcgcggcgga tccaggaagg cgacctcgtc gtgctcgact tcggggcgct
     1321 ggtcgacggc tactgctcgg acatgacccg gacgttcgcc atcggcgaga tcagccccac
     1381 ccagcagaag atgctcgacg tcgtgggcgc ctcgcaggcg gccggggtcg aggcggtggc
     1441 ggcgggcgtc gaggccaagg cggtggacct ggcctcccgg gagatcatcg acgaggcagg
     1501 ctggggcgac gccttcctgc acagcacggg tcacggcgtc ggcctcgaga tccatgagaa
     1561 cccgagggtg gcccagacct ccgaagatac gctccgctcc gactacatcg tgaccgtcga
     1621 gcccggggtc tacctgcccg agcacggcgg ggtccggatc gaggacacgg tcgtggtcac
     1681 cgacgagggt tgccgggtcc tgacccgcgc gcccaagtcg accgatcccc tcgctgcctg
     1741 acaccgactc cctgaaggaa ccgacgagcc gatgcccgcc atcaccacca acgacctgaa
     1801 gaccggcatg cacctggagg tcgaaggcaa cctcgtccag gtcgtggact tccagcacgt
     1861 gaagccgggc aagggccacg ccttcgtgcg caccaccctc aagaacgccc gcaacggcgc
     1921 cgtggtcgag aagacgttcc gggccggcga gaaggtcgag cgggtcaccg tcgacaagcg
     1981 ggagatgcag tacctctaca acgacggcac cgactacgtc ttcatggaca acgccaccta
     2041 cgaccagatc aacgtgagcg ccaagacgct gggcgacgcc gccaactacc tggtcgacgg
     2101 ctcgacggtg atcctgcaga tgttcggctc cgagatcgtg ggcgtcgacc tgcccgccgc
     2161 cgtcgagctg gccatcaccg agtgcgagcc cggcatccag ggggaccggg tctccggcgc
     2221 ccgcaagccg gccacgctgg cgacgggcct gatcgtccag gtgcccctgt tcatcgagcc
     2281 cggcgagcgc atccgggtgg acacccgctc gggcgagtac ctgagccggg cctgagcccg
     2341 tgagcaccga gcggcgccag gcccgcgagc gggccctcgg cctgctctac gaggcggaga
     2401 ccaagagcca gagcccggcc gcactggtgg ccgagcaggt cctcgagccc gatccgttca
     2461 cgaccgatgt ggtgaccggc gtcgaatcgg agggcgagga gatcgacgcc ctcatcaccc
     2521 gcttcgccac gaactggccg ctcaaccgga tgcccgccct ggaccgcacg ctgctgcgca
     2581 tgggcgtcta cgagttgacc caccggccgg acgtgccgac cggcgtggtg ctgtccgaga
     2641 tcgtcgactt ggccagcgag tactccacgg acgactctgg acccttcgtc aacggcgtcc
     2701 tggccgccat cgccgccgag gtccgcaccg atggagatcc ccatccccga tccgccgctg
     2761 gctgagggcg tcgtcgccct ccgaccctgg cggctggagg atgccccggt cctggcgaag
     2821 gcctgggccg atcccgccat ccggcggtgg tgctcggtgc ccgaggatgc caccgtcgac
     2881 ggcgccgaac ggtggatcag cggctcggac cgctgccggt ccgacggcct cgccctcgac
     2941 ctggcggtcg agtgcgacgg tcagctggcc ggcgaggtcg ggctcggacc gatccagtgg
     3001 gcccatcgcc gggccgcggt cggcttctgg gtcgatgccg accatcgtcg ccacaacctg
     3061 gcggccaagg cgctgacgct cctcgccgcc tgggccctgg tcgagctgcc cctcgacacc
     3121 ctcgccggcg aggcctcgtc ggagaacccc gcctccggtt ggaccctgca agccgctgag
     3181 ttccagctcg tcaccgagcg caacacccgc caagcatgga tccgcaccgc ctgacccctt
     3241 ctggcgcccg tggttcgcac tgccccgcga gttcaggttc cgcaacggcg ggttggctca
     3301 ggggaggcgg tcgacggctt ggagccag
//
LOCUS       DASTFV010000066         2708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_215123, whole genome shotgun sequence.
ACCESSION   DASTFV010000066 DASTFV010000000
VERSION     DASTFV010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215123"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1080
                     /locus_tag="VFP02_01215"
     CDS             157..1080
                     /locus_tag="VFP02_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9541669.1"
                     /translation="MRVLVTGGAGFIGANLCRSLLADGHDVRVLDDLSTGRVGNLDGL
                     SLDLVEGSIVEPAVVAGAAADVDRIVHLAARASVPRSIAEPVAAHEANVTGTVNVLEA
                     ARPRGLPVIVASSSSVYGDTAVSPKHEDLPVAPRSPYAASKLATEGYALAYGVAYALP
                     VLALRFFNVYGPLQPADHAYAAVVPRFIDAALAGRPVEIHGDGRQSRDFTFVGSVVGV
                     LGRAVNEATTAPGPVNLAFGTRTDLLDLVARLEALLGQSIAVEHTDPRPGDVRHSTAA
                     GDRLAALFPGVAPINLDEGLAVTVDWFRRRS"
     gene            complement(1102..2448)
                     /locus_tag="VFP02_01220"
     CDS             complement(1102..2448)
                     /locus_tag="VFP02_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9541670.1"
                     /translation="MSQELTGWSRTAPTRATVVSPTTPEALAATLADVGVRGVIARGL
                     GRSYGDAAQNAGGAVADVTGVRRLVAFDGVTGLVTAESGLSFDDLLRVVVPQGWFVPV
                     SPGTRHVTVGGAIAADVHGKNHHRDGSFVQHVRSLRLRTPAGVVEVGPDQDPDLFWAT
                     AGGMGLTGVILDATFALQPISSSRLLVDTERTADLDDAFDRMTRGDHGYHYSVAWIDL
                     VATGATMGRSVLTRGDFAPVDALPEGDRDNPLAYDPTVLVTAPPWVPNLALNRLSVRA
                     FNEFWYRKHPRHRTGELQAIPSFFHPLDFVKGWNRIYGSRGFLQWQMVVPFGAEATLR
                     RAVEALSGEGRPSFLAVLKRFGESDPGPLSFPIAGWTLALDIPAGDATLGPLLDRLDR
                     DVAEAGGRIYLAKDSRLDPALLATMYPRLDEWREIRARVDPDQVLQSDLARRLELL"
     gene            complement(2501..>2708)
                     /locus_tag="VFP02_01225"
     CDS             complement(2501..>2708)
                     /locus_tag="VFP02_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541671.1"
                     /translation="FETAAASSTSVPWFELSILPFVIAVLRYALLLDRGHGAAPEELF
                     FADRPLQLAGLAWALVYGTAVYLT"
BASE COUNT          382 a          964 c         1006 g          356 t
ORIGIN      
        1 atctagcgaa gcgggccggg agagagtcgg catggccgac ggctgccaca ctgaggcccc
       61 catgcgcttc ctcgtcaccg gcgcgaccgg cttcgtcggc tcccacctcg cctggcccct
      121 ggtcgacgtc ccggccatct gggcgaccgc ctagcggtgc gggtcctggt caccggcggg
      181 gcggggttca tcggggccaa tctgtgccgc tcgctgctcg ccgacggcca cgacgtccga
      241 gtgctcgacg acctgtcgac gggccgggtc ggcaacctcg acgggctttc gctcgacctg
      301 gtcgaaggtt cgatcgtcga gcccgccgtc gtcgcggggg cggctgccga cgtcgaccgc
      361 atcgtccacc ttgcggcccg ggcctcggta ccccgctcca tcgccgagcc ggtcgccgcc
      421 cacgaggcca acgtcaccgg caccgtgaac gtcctcgagg cggcccgacc ccggggcctc
      481 cccgtgatcg tggcgtcgtc ctcgtcggtg tacggcgaca ccgccgtctc gccgaagcac
      541 gaggacctcc cggtcgcgcc tcgcagcccc tacgccgcct ccaagctggc gaccgagggc
      601 tacgccctgg cctacggcgt ggcctacgcc ctccccgtcc tggccctgcg cttcttcaac
      661 gtctacggcc cgctccagcc cgccgaccac gcctacgccg ccgtcgtgcc ccggttcatc
      721 gacgccgcgc tggcgggccg gcccgtcgag atccacggcg acggccgcca gagccgggac
      781 ttcacctttg tgggctccgt ggtcggcgtc ctgggccgag cggtgaacga ggccacgacc
      841 gcgcccggcc cggtcaacct ggccttcgga acccggaccg acctcctcga cctcgtcgcc
      901 cgcctggaag ccctcctcgg ccagtcgatc gccgtggagc acaccgaccc ccgccccggc
      961 gatgtccgcc actcgacggc ggcaggggac cgactggcgg ccctgttccc cggcgtggcg
     1021 ccgatcaacc tcgacgaggg cctggcggtc accgtcgact ggttccgccg ccgatcctga
     1081 gcggtcggcc gatccggctc gtcagagcag ttcgaggcgc cgggcgaggt cggactggag
     1141 gacctggtcg gggtcgacgc gggcgcggat ctcccgccac tcgtcgaggc gggggtacat
     1201 ggtggccagc agcgcggggt cgaggcgact gtccttggcc aggtagatgc ggccaccggc
     1261 ctcggccacg tcgcggtcga ggcggtcgag caacgggccc agggtggcgt cgccggcagg
     1321 gatgtccagg gccagggtcc agcccgcgat cgggaacgac aggggtccag ggtcgctctc
     1381 gccgaaacgc ttgagcacgg ccaggaacga cggacgcccc tcacccgaga gggcctcgac
     1441 ggctcggcgc agggtggctt cggctccgaa gggcacgacc atctgccact gcaggaagcc
     1501 ccggctgccg tagattcggt tccagccctt gacgaaatcc aaggggtgga agaacgaggg
     1561 aatggcctgg agctcgcccg tccggtggcg agggtgcttg cggtaccaga actcgttgaa
     1621 ggcccggacc gacagccggt tcagggcgag gttgggcacc cagggcggag cggtcacgag
     1681 gacggtgggg tcgtaggcca gggggttgtc ccggtcgccc tcgggcaggg cgtcgaccgg
     1741 ggcgaagtcg ccacgggtca gcacggaccg acccatggtg gccccggtgg cgacgaggtc
     1801 gatccacgcc accgagtagt ggtagccgtg gtcgccgcga gtcatgcggt cgaaggcgtc
     1861 gtccaggtcg gcggtccgct cggtgtcgac caggaggcgg ctgctgctga tcggctgcag
     1921 ggcgaaggtg gcgtcgagga tgacaccggt caggcccatg cccccggccg tggcccagaa
     1981 gaggtcgggg tcctggtcag gaccgacctc gacgacaccg gccggtgtgc gcaggcgcag
     2041 cgagcggacg tgctggacaa aggagccgtc ccggtggtgg ttcttgccgt ggacgtcggc
     2101 ggcgatggcc cccccgaccg tgacgtggcg ggtgccgggg gagacgggca cgaaccagcc
     2161 ctggggcacg accacccgca ggaggtcgtc gaagctcagg cccgactcgg cggtgaccaa
     2221 accggtgacg ccgtcgaagg ccaccaggcg gcgcaccccg gtgacgtcgg ccaccgcccc
     2281 accggcgttc tgggcggcgt cgccgtagga gcgacccagg ccgcgagcga tcacgccccg
     2341 gaccccgacg tcggccaagg tggcggcgag ggcctccggg gtggtggggg agaccaccgt
     2401 ggcccgggtc ggggccgtgc gcgaccagcc ggtcagctcc tggctcatcg ttgatcctgt
     2461 cggcggcggg ggctggccac gacagggttc cgtcccggca tcacgtcagg tagacggccg
     2521 tgccgtagac cagggcccag gccaggccgg ccagctggag cggccgatcg gcgaagaaca
     2581 gctcctcggg ggcggcgccg tggccgcgat cgaggagcag ggcgtagcga aggacggcga
     2641 tcacgaaggg caggatcgag agctcgaacc acggcaccga agtcgagctg gccgcagccg
     2701 tctcgaag
//
LOCUS       DASTFV010000067         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_218777, whole genome shotgun sequence.
ACCESSION   DASTFV010000067 DASTFV010000000
VERSION     DASTFV010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218777"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VFP02_01230"
     CDS             complement(<1..350)
                     /locus_tag="VFP02_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9541672.1"
                     /translation="MSGICEGRVCVITGAGRGIGREYALMLADQGAKIVVNDLGGDMT
                     GEGEDATPAQAVVAEIEAMGGEAVANGANVADFDASGEMIQQAIDHFGRLDVLINNAG
                     ILRDRMLVNMAEGE"
     gene            484..2145
                     /locus_tag="VFP02_01235"
     CDS             484..2145
                     /locus_tag="VFP02_01235"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HET9541673.1"
                     /translation="MRRPSPRKAVALMIPLVIVAAAVLTWVVDTRIHSDLVARNVVLD
                     GRAIGGFGQEDIDARVRHLSSTYLDTPVEIHSGDRTLETTVGELGAEVDQAATVAAAQ
                     DIGQDPLSWAERFREDEVAPVILTLADNPVSRTLQDLEGSDRSSPIEPLIQHSGFGFA
                     VVPGVPGHGIRATEVIDQVVAGAVAGEDPIVVDVDAKDIPPRFDDSEAEALAEEANTL
                     TEDGITINADGTSADVTAETLGPWVTSRPGREALQLVLDREQVGEDLPGLLPDVGDPP
                     AAASFTVSNGRPRIVPGQPGTGCCAPGSARRVIAALRNGDRSVDLELTRIRPERDAEW
                     AESLGIVEEISLPDQEPCTAYAAEGCRMSTHHDCCESRVTNIHRMADIVRGYVIPPNG
                     GHFSINEVVGPRTTENGFVVAGAIENGEHVESVGGGVSQFATTSFNAAFFAGLDIPNY
                     QFHTEHLSRYPYGRESTVSYPAPEFRIVNNTPYGVLMWPTYDDTTITMHLYSTRFATG
                     AQTGQSTGTNGSCTTVNTTRTRTYVDGHTENDTFSGYYRNGGTHC"
     gene            2183..>2565
                     /locus_tag="VFP02_01240"
     CDS             2183..>2565
                     /locus_tag="VFP02_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET9541674.1"
                     /translation="MTDEHLDFQPRMSDADKLMWTIERDPLLRSTITSVVVLDGPPDH
                     ARLKDKVERASRTVIRLRQRVIGNALSFSPPRWEVDPNFDLDYHLRFVKAANDGSMRE
                     LFDIAEPIGMQAFDRARPLWEFTVV"
BASE COUNT          416 a          965 c          820 g          364 t
ORIGIN      
        1 cactccccct cggccatgtt caccagcatc cggtcccgca ggatcccggc gttgttgatg
       61 agcacgtcga ggcggccgaa gtggtcgatg gcctgctgga tcatctcacc cgaagcgtcg
      121 aagtcggcga cgttggcccc gttggccacg gcctcgccgc ccatggcctc gatctcggcc
      181 acgaccgcct gggccggggt ggcgtcctcg ccctccccgg tcatgtcccc gccgaggtcg
      241 ttgacgacga tcttggcgcc ctggtcggcc agcatcaggg cgtactcccg cccgatgccc
      301 cggccggcgc cggtgatcac acagacccga ccttcacaga tcccgctcat gtcctcgtcc
      361 tcgttcgcgc tcgacgtgct ggccaccaag tgggcgagag agtgtttcac cgacgggccg
      421 ggaggggcga atcccccggg ggtgacagag gatcagcccc cgcgcacgcc agaatggccg
      481 ggaatgcgtc gtccgtcccc ccgcaaggcc gtcgcgctca tgatcccgct ggtgatcgtg
      541 gccgcggccg tcctgacttg ggtggtcgac acgcgcatcc actccgacct ggtcgcccgc
      601 aacgtcgtcc tcgacggccg ggcgatcggt ggtttcggcc aggaggacat cgacgcccgg
      661 gtgcgccacc tctcctcgac ctacctcgac acgccggtcg agatccactc cggggaccgc
      721 acgctcgaga ccacggtggg cgagctgggc gcggaggtcg accaggcggc cacggtcgcc
      781 gccgctcagg acatcggcca ggaccccctg tcctgggccg agcgcttccg ggaggacgag
      841 gtggccccgg tcatcctgac cctcgccgac aacccggtga gccgcacgct gcaggacttg
      901 gaggggtccg atcgcagcag cccgatcgag ccgctcatcc agcactccgg cttcggcttc
      961 gccgtcgtgc cgggtgtgcc gggccacggc atccgggcga ctgaggtcat cgaccaggtc
     1021 gtcgccgggg ccgtcgccgg agaggacccc atcgtcgtcg acgtcgacgc gaaggacata
     1081 ccgccccgct tcgacgacag cgaggccgag gcgctcgcgg aagaggccaa caccctcacc
     1141 gaggacggga tcaccatcaa cgccgacggc acctccgccg acgtgaccgc agagacgctg
     1201 gggccctggg tcacgtcccg gccgggacgg gaggccctgc agctggtgct cgaccgcgag
     1261 caggtcggcg aggacctgcc cggcctgctg cccgacgtcg gtgacccccc agccgccgcc
     1321 agcttcacgg tgagcaacgg tcgcccccgc atcgtcccgg gccagcccgg cacgggctgc
     1381 tgcgcgcccg gctcggcccg tcgggtcatc gccgccctgc gcaacggcga ccgatcggtg
     1441 gacctcgagc tgacccggat ccggcccgag cgcgacgccg agtgggccga gagcctgggc
     1501 atcgtcgagg agatcagcct cccggaccag gagccgtgca ccgcctacgc cgccgaaggc
     1561 tgccgcatga gcacccacca cgactgttgt gagagccggg tcacgaacat ccaccggatg
     1621 gccgacatcg tgcggggcta cgtgatccct ccgaacggag gccatttctc catcaacgag
     1681 gtcgtcgggc cccggacgac cgagaacggc ttcgtggtcg ccggcgccat cgagaacggc
     1741 gagcacgtcg agtccgtcgg tgggggggtg tcccagttcg ccaccacctc gttcaacgcc
     1801 gccttcttcg ccgggctcga catcccgaac taccagttcc acaccgagca cctcagccgc
     1861 tacccgtacg gacgcgagtc gaccgtgtcc tacccggcgc ccgagttccg catcgtgaac
     1921 aacacgccgt acggcgtgct catgtggccc acgtacgacg acaccaccat caccatgcac
     1981 ctctactcca cccggttcgc caccggagcc cagaccggac agtcgacggg cacgaacggc
     2041 agctgcacca cggtcaacac cacgaggacc cggacctacg tcgacggcca caccgagaac
     2101 gacacgttca gcggctatta ccgcaacggc ggcacccact gttgatggtg acgtcgcctc
     2161 ggatatgagg tgaactggag aggtgaccga cgagcacctc gacttccagc cccgcatgag
     2221 cgacgccgac aagctcatgt ggaccatcga gcgggatccg ctgctccgga gcacgatcac
     2281 ctccgtcgtc gtcctcgacg gtccccctga ccacgcccgc ttgaaggaca aggtggagcg
     2341 ggccagccga accgtcatcc gcctgcgcca gcgagtcatc ggcaacgccc tgtccttctc
     2401 gccgccccgc tgggaggtcg atcccaactt cgacctcgac taccacctgc gcttcgtgaa
     2461 ggcggccaac gacggcagca tgcgcgagct gttcgacatc gccgagccca tcggcatgca
     2521 ggccttcgac cgggcccggc cgctgtggga gttcaccgtg gtcga
//
LOCUS       DASTFV010000068         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_222897, whole genome shotgun sequence.
ACCESSION   DASTFV010000068 DASTFV010000000
VERSION     DASTFV010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222897"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..761
                     /locus_tag="VFP02_01245"
     CDS             78..761
                     /locus_tag="VFP02_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9541675.1"
                     /translation="MAESQTAAPSRLEILKALGENTRYAIYLELARSPRPLATAEIAA
                     TLALHANTVRPHLERMREVGLLDQRIDNNGGVGRPQHLYSLAPDAPSLGLEPPAMPAL
                     ARMLLTVAAEAGLDGNDAEAAGRDQGARDAESFPEDSPCIEALMTQLEYLGFDPEATG
                     GADGVTVAFTHCPFRELAEANPDLVCGLHRGMVEGFVDGLGTGQMGAFRTLVDRVPCQ
                     TDVVELRVR"
     gene            796..1116
                     /gene="erpA"
                     /locus_tag="VFP02_01250"
     CDS             796..1116
                     /gene="erpA"
                     /locus_tag="VFP02_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HET9541676.1"
                     /translation="MITVTDSAAAKVKELLEQEDDADLALRVAVRPGGCSGFSYEMFF
                     DSDVGDDDTKATFGQVEVVVDASSAMLLEGATLDYKDGLNQAGFAIDNPNAERTCGCG
                     QSFS"
     gene            complement(1126..>2012)
                     /locus_tag="VFP02_01255"
     CDS             complement(1126..>2012)
                     /locus_tag="VFP02_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541677.1"
                     /translation="PGISRRTVLAGGAALAFLTACSGGDDGDSADGTPDDSTPGPDEL
                     TGTLLVLLPPQGVLITDGEQRVPVAVADAEGVPVRAGLEPRDFQIRTDTGEPTVVTVE
                     PHSDGVPTPYYPIRFTPAGAGIHEITVVDDPGFNAVTLDIGDSTLIPIPGQALPVEHT
                     PTPANARGVDPICTRVPACALHDVDLADALGAGPVALVIATPEFCQTAICGPVLDLVI
                     EALPDYPTITAIHAEVYADPLSGDDPRAGGLAPLTDAFALSFEPTLYLMDATGTVVTR
                     LDSVYDRVELREALDQIA"
BASE COUNT          292 a          700 c          731 g          289 t
ORIGIN      
        1 aagacgtcct cgctgcgggg cagggtcgtc ggggccatgg ggtgatttta ccggcgtagc
       61 ccgtgtaatc tggtcccatg gccgagtcgc agaccgctgc cccctctcgg ctcgagatcc
      121 tgaaggcact gggcgagaac acccggtacg ccatctacct ggagctggct cgctcgcccc
      181 gccccctggc caccgcagag atcgccgcca ccctcgccct ccacgccaac acggtgcgcc
      241 cccacctgga gcgcatgcgc gaggtcggtc tgctcgacca gcgcatcgac aacaacggcg
      301 gggtcggccg gccccagcac ctctactcgc tggcccccga cgcgccgtcg ctgggcctcg
      361 agcccccggc catgcccgcg ctggcccgca tgctgctcac cgtcgccgcc gaggccggcc
      421 tcgacggcaa cgacgccgag gccgccggtc gggaccaggg ggcccgggac gccgagagct
      481 tccccgagga cagcccttgc atcgaggccc tgatgaccca gctcgagtac ctcggcttcg
      541 accccgaggc cacggggggc gccgacggcg tgaccgtcgc cttcacccac tgccccttcc
      601 gcgagctggc ggaggccaat cccgacctgg tgtgcggcct gcaccggggc atggtcgagg
      661 ggttcgtgga cggtctgggc accgggcaga tgggggcgtt ccgcaccctg gtcgaccggg
      721 tgccctgcca gaccgatgtg gtcgaactcc gcgttcggta gattgattgc acagacaagc
      781 acgggaggac caccagtgat caccgtcacc gacagtgcgg ccgccaaggt caaggagctc
      841 ctcgagcaag aggacgacgc cgaccttgcg ctgcgcgtcg cggtgcggcc gggtggttgc
      901 tcgggcttca gctacgagat gttcttcgac agcgatgtcg gcgacgacga caccaaggcc
      961 accttcggcc aggtcgaggt cgtcgtggac gcctccagcg ccatgctgct cgagggggcg
     1021 accctcgact acaaggacgg cctgaaccag gcggggttcg ccatcgacaa ccccaacgcc
     1081 gagcgcacct gcggctgcgg ccagtccttc agctagaccg cctcctcagg cgatctgatc
     1141 cagcgcttcg cgtagttcga cgcggtcata gacgctgtcg aggcgggtga cgaccgtgcc
     1201 ggtggcatcc atcaggtaca gcgtcggctc gaaggacagg gcgaaggcgt cggtgagggg
     1261 ggccaggccc cccgctcggg ggtcgtcgcc gctcaagggg tcggcgtaga cctcggcgtg
     1321 gatggcggtg atcgtcgggt agtcgggcag ggcctcgatg accaggtcca gcaccggccc
     1381 gcagatcgcc gtctggcaga actcgggggt ggcgatcacg agggcgaccg gccccgcccc
     1441 cagggcgtcg gccaggtcga cgtcgtgcag ggcacacgcc ggaacgcggg tgcagatcgg
     1501 gtcgacccct cgggcgttcg ccggcgtcgg ggtgtgttcg acgggcaggg cttggcccgg
     1561 gatcgggatg agcgtgctgt cgccgatgtc gagcgtgacg gcgttgaacc cggggtcgtc
     1621 gaccacggtg atctcgtgga tcccggcgcc ggccggcgtg aagcggatcg ggtagtacgg
     1681 cgtggggacg ccgtcgctgt ggggctcaac cgtcacgacg gtcggctccc cggtgtcggt
     1741 tcggatctgg aagtcccgcg gctcgagacc tgcccggacc ggcacccctt cggcgtcggc
     1801 gacggccacc ggcacccgct gctcgccatc ggtgatgagc acgccctggg ggggcaggag
     1861 caccagcaag gtgccggtga gctcgtcggg gcccggcgtg ctgtcgtcgg gagtgccgtc
     1921 ggccgagtcg ccgtcatcgc ccccgctgca ggcggtgagg aacgccaggg cggcaccgcc
     1981 ggccaagacc gtccgtcggg agattccggg ag
//
LOCUS       DASTFV010000069         3575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_223608, whole genome shotgun sequence.
ACCESSION   DASTFV010000069 DASTFV010000000
VERSION     DASTFV010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223608"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="VFP02_01260"
     CDS             <1..1210
                     /locus_tag="VFP02_01260"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9541678.1"
                     /translation="DAPKLVRRDDGTDVWRYGDAELPNIGLNAVAGRPPAEYGIDPTS
                     FADMREGCYDIAARIADMNANGVLGSMCFPSFPQFCGQLFARTEDKDVALAMVRAYND
                     WHIDEWCGYEPGRFIPLAIPAVWDPQVMADEVRRVAAKGCHAVTFSENPERLGWPSFH
                     SDHWDPFWAACSDTGTVVCLHIGSSSQMIMTAADAPINVMITLQPINLVQAASDLLWS
                     RVFHKFPDVRFALSEGGIGWIPYFLERADYVYQHHREWTGTDFGDQLPSQTFLEKSIF
                     CFIDDAFGLESRHHIGVDRICWECDYPHSDSTWPTSPELLSKSLHGIPDAEVNQMTHE
                     NAMRFFQFDPFAHRPREQATVGALRAEAAGVDTSLRGSGKAPRDPSEGPITILDIVNA
                     ASANGATAAQ"
     gene            complement(1232..2383)
                     /locus_tag="VFP02_01265"
     CDS             complement(1232..2383)
                     /locus_tag="VFP02_01265"
                     /inference="COORDINATES: protein motif:HMM:NF005892.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541679.1"
                     /translation="MTIKDATAIVGIGQTEWGKGLEDAELVLALRAIKAALDDAGIEP
                     GEVDGLSSYTLETSDEVDIARNLGLGDITWFSQVGYGGGAGCGVVGHAAVAVATGQAN
                     VAVAWRSRKRGAAASRPWSQVASRVGGSHQWSRPFGLLRPVDEIAMLARRYFHEFGGT
                     REELAAVAVTVREHANRNPAAMMYGKALTVEQYLEARWISEPLCLFDNCLESDGAAAV
                     VIVSAARALDLDVTPALIHSFAQSIPQQHQVMTNYFGDDPLKGPAWACADLLWRNSDL
                     TPADVHVAQLYDAFSPLVPLSLEGYGFCERGEGLAFCQDGGISWDGGRLPVNTSGAGM
                     SEAYVHGFNLITEAVRQVRGTSTAQVDGASVSLVTSGEGVPTSALLLRA"
     gene            complement(2380..>3575)
                     /locus_tag="VFP02_01270"
     CDS             complement(2380..>3575)
                     /locus_tag="VFP02_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET9541680.1"
                     /translation="VCIYAISGGTGAHLADLASADGLRIPDLTEATQTALREWIPGYL
                     RVSNPVDCGGAPSADWRGRKILDTLLADPNVGVLVCPITGALPTMSRPLAEDLVAVAE
                     TTDKPIVVIWGSPLADDPAYVDVLVPSGLPVFRTFGNCVGAVKAYLDHHEFRDRHTSP
                     FAKPVRRRSPAAPLVDALLSDPRSGTGPLSEFESKGVLAAYGIPVTDDKLCHSAAAAV
                     KAASELGYPVVMKACSADLAHKSDLGLVVVGIGSAAEVRRTYADLVERSPVVPDGILV
                     CPTAAPGVECVVGVSQDALFGPVVMFGLGGVLVEVLGDVTFRVPPFDRAEAGRMICEV
                     KGFPLLTGVRGHPKADLKALVDVILKVQRLAVDHTETIAELDINPLVVHPTGATALDA
                     LIVTR"
BASE COUNT          545 a         1260 c         1275 g          495 t
ORIGIN      
        1 cgacgcaccg aagctcgtcc ggcgcgacga cggcaccgac gtgtggcggt acggggacgc
       61 ggagctaccc aacatcgggc tcaacgcggt cgccggccgc ccgcccgccg agtacggcat
      121 cgaccccacg tcgttcgccg acatgcggga gggttgctac gacatcgctg cccgcatcgc
      181 cgacatgaac gccaacggtg tcctcggctc gatgtgcttc ccgtcgttcc cgcagttctg
      241 cggccagctc ttcgcccgga cggaggacaa ggacgtggcc ctggccatgg ttcgggccta
      301 caacgactgg cacatcgacg agtggtgcgg gtacgagccg gggcgcttca tccccctggc
      361 catccccgcc gtctgggacc cgcaggtcat ggccgacgag gtccgacggg tggcggccaa
      421 gggctgtcac gccgtgacct tctcggagaa ccccgagcgt ctcggctggc cgagcttcca
      481 cagcgaccac tgggacccgt tctgggcggc ctgctcggac accggcaccg tggtctgcct
      541 gcacatcggg tcgtcgtcgc agatgatcat gacggcggcc gacgccccca tcaacgtgat
      601 gatcacgctg cagccgatca acctggtcca ggccgcgtcg gacctgctgt ggtcccgggt
      661 gttccacaag ttccccgacg tgcgcttcgc cctgtccgag ggcggcatcg gctggatccc
      721 gtacttcctg gagcgggccg actacgtgta ccagcaccac cgcgaatgga ccggcaccga
      781 cttcggcgac cagctcccca gccagacctt cctcgagaag tcgatcttct gcttcatcga
      841 cgacgccttc gggctggaga gccggcatca catcggcgtc gaccgcatct gctgggagtg
      901 cgactacccg cactcggact ccacctggcc cacgtcaccg gagctgctga gcaagtcgct
      961 gcacggcatc ccggacgccg aggtcaacca gatgacccac gagaacgcca tgcggttctt
     1021 ccagttcgac ccgttcgccc accggccccg cgagcaggcg acggtggggg ccctccgggc
     1081 cgaggccgcc ggcgtcgaca cctcgctgcg gggctcgggc aaggcgcccc gcgacccgag
     1141 cgagggcccc atcaccatcc tcgacatcgt caacgccgcc agcgccaacg gcgccaccgc
     1201 cgcccagtaa cccggcgagc cggaccccgg gtcaggcccg cagcaacagc gccgaggtgg
     1261 gcacgccttc gcccgaggtg acgagggaga ccgacgcgcc gtcgacctga gcggtcgagg
     1321 ttccgcgcac ctggcggacg gcttcggtga tgaggttgaa gccgtggacg taggcctcgg
     1381 acatgccggc gccggaggtg ttcaccggca accgcccacc gtcccaggag atgccgccgt
     1441 cctggcagaa ggccagtccc tcgccccgct cgcagaagcc gtagccctcc agggacagcg
     1501 ggacgagggg cgagaaggcg tcgtagagct gggccacgtg gacgtcggcg ggggtcaggt
     1561 ccgagttgcg ccacagcagg tcggcgcagg cccaggccgg gcccttcaag gggtcgtcgc
     1621 cgaagtagtt ggtcatgacc tggtgctgct gggggatcga ctgggcgaag gagtggatga
     1681 gcgccggcgt gacgtcgaga tcgagggccc gcgccgccga gacgatgacc acggcggcgg
     1741 cgccgtccga ctcgaggcag ttgtcgaaca ggcagagcgg ctcggagatc cagcgggcct
     1801 cgaggtactg ctcgaccgtc agtgccttcc cgtacatcat cgccgccggg ttccggttgg
     1861 cgtgctcgcg aacggtgacg gcgaccgcgg ccagctcctc ccgggtgccg ccgaactcgt
     1921 ggaagtagcg gcgggccagc atggcgatct cgtcgaccgg ccggaggagg ccgaagggcc
     1981 ggctccactg atgcgagccg ccgacccggc tggctacctg gctccacgga cggctggcgg
     2041 cggccccccg cttgcggctg cgccaggcca cggccacgtt cgcctgtccg gtggcgacgg
     2101 cgacggcggc atggccgacg accccacaac cggcccctcc gccgtagccg acctggctga
     2161 accaggtgat gtcgccaagg cccaggttgc gggcgatgtc gacctcgtcg ctggtctcca
     2221 gcgtgtacga cgacaggccg tcgacctcgc ccggctcgat gcccgcgtcg tccagcgcgg
     2281 ccttgatggc tcgcagcgcg aggaccagct cggcgtcctc cagccccttg ccccactcgg
     2341 tctggccgat gcccacgatg gcggtggcgt ccttgatcgt catcgggtga cgatcagggc
     2401 gtcgagggcg gtggcgccgg tggggtgcac gacgagtggg ttgatgtcga gctcggcgat
     2461 ggtctcggtg tggtcgacgg cgagccgctg gaccttcagg atcacgtcga ccagcgcctt
     2521 caggtcggcc ttggggtgtc cccgcacgcc ggtgagcagc gggaacccct tgacctcgca
     2581 gatcatgcgg cccgcttcgg cccggtcgaa cggggggacc cggaaggtga cgtcgccgag
     2641 gacctcgacc agcacgccgc ccaggccgaa catcaccacg gggccgaaca gcgcatcctg
     2701 ggacacgccc acgacgcact cgactcccgg cgcggccgta gggcacacga ggatgccgtc
     2761 ggggacgacg ggggagcgct cgaccaggtc ggcataggtc cgtcgcacct cggccgccga
     2821 cccgatgccg acgacgacca ggccgagatc gctcttgtgg gccaggtcgg ccgagcacgc
     2881 cttcatcacc accgggtagc cgagctccga ggccgccttc accgccgcgg ccgccgagtg
     2941 gcacagcttg tcgtcggtca ccgggatccc gtatgcggcc agcaccccct tcgactcgaa
     3001 ctccgacagc ggtcccgttc cagaacgggg gtcggagagg agggcgtcga cgaggggcgc
     3061 ggcgggggag cggcggcgga cgggcttggc gaacggcgac gtgtggcggt cccggaactc
     3121 gtggtggtcg aggtacgcct tcaccgcgcc cacgcagttc ccgaaggtgc ggaacacggg
     3181 gaggcccgag ggcaccagca cgtcgacgta ggccgggtcg tcggccaaag gcgagcccca
     3241 gatgacgacg atcggcttgt cggtggtctc ggccacggcg accaggtcct cggccagggg
     3301 tcggctcatg gtcggcaggg ccccggtgat gggacagacg agcacgccca cgttggggtc
     3361 ggcgagcagg gtgtcgagga tcttgcgccc ccgccagtcg gcggacgggg cgccgccgca
     3421 gtcgaccggg ttcgacactc gcaggtagcc ggggatccac tcccgcagcg cggtctgggt
     3481 ggcctcggtc aggtcgggga tcctcaggcc gtcggccgaa gccaggtcgg ccaggtgggc
     3541 cccggtgccg ccggagatgg cgtagatgca gaccc
//
LOCUS       DASTFV010000070         2569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_224004, whole genome shotgun sequence.
ACCESSION   DASTFV010000070 DASTFV010000000
VERSION     DASTFV010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224004"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..884)
                     /locus_tag="VFP02_01275"
     CDS             complement(123..884)
                     /locus_tag="VFP02_01275"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET9541681.1"
                     /translation="MSIVTPEAVRLEFAPAGVATRMLGLFIDLAIQLAALLALGLVVG
                     LTTSGGVSETAAVILTLVGLFLIILGYPVACESLWNGRTVGKAALGLRVRTKEGAPIR
                     FRHAAIRGALGLVDFWLTLGAAATISVLVTRDSQRLGDLAAGTLVLRERTAASLTSSA
                     FAFYPPPGWEAYAAALDVGAMTADQYGLVRGYLLRASEMTPAARHHLALRLANPLTVI
                     LAHQPPAGVQAEPFLHCAAAAYQRLHRAPERTPPY"
     gene            877..2007
                     /locus_tag="VFP02_01280"
     CDS             877..2007
                     /locus_tag="VFP02_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HET9541682.1"
                     /translation="MLTAALPRRAASTGRPRHEPNLRPLDIDGFIDRNQPTWQRLADR
                     TGRARRAVRRLGPGELDQLVADYQRTSAHLSYARTAFADPGLTSRLTRLVADANGVIY
                     GKRNRTLSSIGRFFAVTFPAAVWYARRFVLVSALLMVVPAVAVGAWIGTSDDALDAAA
                     PDAVREAYLDEGFEAYYSSEPAAQFATEVTVNNIQVSILAFAAGIAFCLGAAYVLILN
                     GANLGVAAGLVVAAGQSPKFFGLILPHGLLELTAISLAAAAGIQMGWALIAPGDRTRG
                     DAVAEEGRRSVVIVLGLALAFVVAGTIEGFVTGSGLSTLVRVGIGVTVEIAFVLYVVV
                     QGRRAAAVGETGLLGERPPTWGDEWSLHRSASTPTTVLSPSP"
     gene            complement(1979..>2569)
                     /locus_tag="VFP02_01285"
     CDS             complement(1979..>2569)
                     /locus_tag="VFP02_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541683.1"
                     /translation="RVMAGQVGDVPRVEHAMDAVMMLTAVATRLGDRAGLVAFDHEVR
                     AVVSPGHGPGQLGRVTEAMYRLEPALVESDYRGAFTATLARFRRRALLVVLTELVEQA
                     VGEFLAPALPLICARHLVVIGAVQDPEVLAWANATPTGTEEAYRKASAVAALQERDRT
                     IARLQGLGATVVDAAPGRLAPQLADAYLKVKATGRL"
BASE COUNT          339 a          952 c          940 g          338 t
ORIGIN      
        1 cggcatcgac gctgcgcagt ccaggtaggc ggtgctcacc ccctcaggct acggacccac
       61 tccttccggt tgcgtccatc cgcggccgac cgcgggaagg tgcaaccaga accgggggtg
      121 actcagtagg ggggtgtgcg ctcgggggcg cggtggaggc gctggtaggc ggcggccgcg
      181 cagtggagga agggctcggc ctgcacgccc gccggtggct ggtgggcgag gatcaccgtc
      241 agcgggttgg ccaggcgcag ggcgaggtgg tggcgggcgg cgggggtcat ctccgacgcc
      301 cgcaggaggt agccccggac caggccgtac tggtcggcgg tcatggcccc gacgtcgagc
      361 gcggccgcgt aggcctccca gccgggcggc gggtagaagg cgaaggcgga cgacgtcagc
      421 gaggccgcgg tgcgctcccg caggacgagg gtgccggcgg cgaggtcccc caggcgctgg
      481 ctgtcccggg tcaccagcac cgagatcgtg gccgccgccc cgagggtgag ccagaagtcc
      541 accaggccca gcgcgccccg gatggcggcg tgccggaacc ggatcggggc cccctccttg
      601 gtccgcaccc gcagcccgag ggcggccttg cccacggtgc gcccgttcca cagcgactcg
      661 cacgccaccg ggtacccgag gatgatcagg aagagcccga cgagggtcag gatcaccgcc
      721 gcggtctcgg acacgccgcc ggacgtggtc aggccgacga cgaggccgag ggcgagcagg
      781 gcggccagtt ggatggccag gtcgatgaac aggcccagca tccgggtggc cacgccggcg
      841 ggggcgaact ccagacggac ggcctcgggc gtgacgatgc tcacggccgc gctcccccgt
      901 cgtgctgcct ccaccggccg gccacgccac gaacctaacc tcaggcccct ggacatcgac
      961 ggcttcatcg accgcaacca accgacctgg cagcggttgg cggaccggac cgggcgggcc
     1021 cgtcgggctg tacggcgact cgggccgggg gagctggacc agctggtcgc cgactaccag
     1081 cgcacctcgg cgcacctgtc ctacgcccgg accgccttcg ccgatcccgg cctgacctcc
     1141 cgcctgaccc gcctggtggc cgacgccaac ggggtcatct acggcaagcg caaccgcacc
     1201 ctgtcctcga tcgggcggtt cttcgccgtc accttccccg ccgcggtctg gtacgcccga
     1261 cgattcgtgc tggtcagtgc cctgctcatg gtggtccctg ccgtcgcggt cggcgcctgg
     1321 atcgggacgt cggacgacgc cctcgacgcc gcggcccccg acgcggtgcg ggaggcgtac
     1381 ctcgacgagg gcttcgaggc gtactactcg tcggagccgg cggcgcagtt cgccaccgag
     1441 gtcaccgtca acaacatcca ggtgtcgatc ctggccttcg ccgccgggat cgccttctgc
     1501 ctgggcgcgg cctacgtgct gatcctgaac ggggccaact tgggcgtggc ggccgggctg
     1561 gtcgtggccg cgggccagtc ccccaagttc ttcggcctga tcctgcccca cggcctcctc
     1621 gagctcaccg ccatctccct ggccgcggcc gccgggatcc agatgggctg ggccctcatc
     1681 gcccccggcg accggacccg gggagacgcc gtcgccgaag agggccgtcg ctcggtggtc
     1741 atcgtcctcg gtctggccct cgccttcgtc gtggccggca ccatcgaggg cttcgtcacg
     1801 ggaagcgggc tgtccaccct cgtccgggtc ggcatcggcg tcaccgtcga gatcgccttc
     1861 gtgctctacg tggtcgtcca gggccggcgg gcggcggccg tgggcgagac cggcctcctc
     1921 ggcgagcgcc cgcccacctg gggcgacgag tggtcgctcc atcgttcggc ctccacccct
     1981 acaaccgtcc tgtcgccttc accttgaggt aggcatcggc cagctggggg gcgaggcggc
     2041 cgggagcggc gtcgaccacg gtggccccga ggccctggag ccgggcgatg gtgcgatccc
     2101 gctcctggag ggcggccacc gccgacgcct tgcgataggc ctcctcggtg cccgtcggcg
     2161 tggcgttggc ccacgccagg acctcggggt cctgcacggc gccgatgacc acgaggtggc
     2221 gggcacagat caggggcaga gccggggcca ggaactcccc caccgcctgc tcgaccagct
     2281 cggtgaggac gaccagcagg gcccggcgcc ggaaccgggc caacgtggcc gtgaaggcgc
     2341 cccggtagtc gctctcgacc agagcgggct ccaggcggta catggcctcg gtcacccggc
     2401 ccagctggcc ggggccgtga cccgggctga ccacagcccg gacctcgtgg tcgaaggcga
     2461 cgagcccggc ccggtcgccc agccgggtgg ccacggccgt cagcatcatc accgcatcca
     2521 tggcgtgctc gacccggggg acgtcgccca cctggcccgc catcacccg
//
LOCUS       DASTFV010000071         4151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_233089, whole genome shotgun sequence.
ACCESSION   DASTFV010000071 DASTFV010000000
VERSION     DASTFV010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233089"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /gene="topA"
                     /locus_tag="VFP02_01290"
     CDS             complement(<1..1480)
                     /gene="topA"
                     /locus_tag="VFP02_01290"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET9541684.1"
                     /translation="MANPLVIVESPAKAKTIAGYLGSGYDVDSSVGHIRDLPQNAADV
                     PAAYKGEPWARLGVDTDNDFKPLYVVNKGKKDVVANLRKKMKAASELYLATDEDREGE
                     AIAWHLLEVLNPPSGMPVRRMVFHEITPEAIQRAIDEPREIDRRLVDAQEARRILDRL
                     YGYEVSPVLWKKVQSGLSAGRVQSVATRIVVERERARMRFTVASYWDLDGRFAGGEPQ
                     ADFGATLVAVDGARLATGKDFDEDGRLQGDVTLLDEAGARGLASALDRADVTVRSVES
                     KPYTRKPYAPFMTSTLQQEAGRKLRFSSARTMSVAQRLYENGYITYMRTDSTTLSDTA
                     VAAARAQILEKYGRDHLPDAPRQYTRKVKNAQEAHEAIRPAGDRFRLPEEVQRELQGD
                     EFRLYELVWRRTVASQMTDARGQSVQVRLGAMASDGRDAEFAATGKTIHHPGFLRAYV
                     EGSDDPEGDLEDQERILPDMGEGDQVAVRSIEAKGHETQPPAR"
     gene            complement(1548..2306)
                     /locus_tag="VFP02_01295"
     CDS             complement(1548..2306)
                     /locus_tag="VFP02_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9541685.1"
                     /translation="MSVDGAVVLVTGGAGGIGAAAARMFARDGAETVVIADVKDASET
                     VAAVQAAGVTAEWIDADLAEEDVVVDLIARIVADHGRLDTAFNNAGITGHMTTFHELD
                     KATWDRMIAVNLTSVFLCMKHELAHMVERGGGGAIVNTSSGAGVFGFAGLPHYVAAKH
                     GVLGLTRTAASEYARQGIRVNAVLPGPTETPMLLSFMGEDDGLRQLMASSVPSGELLQ
                     PEDIAEAVVWLASDKARQVSGQAHMIDGGSIHSR"
     gene            2349..3560
                     /locus_tag="VFP02_01300"
     CDS             2349..3560
                     /locus_tag="VFP02_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9541686.1"
                     /translation="MDFEPSDKCRELSERLEAFMAEKVIPAEPVYAEQMAASGDGHHH
                     PQVMEDLKAEARARGLWNLFLPHATEWTEGLSNLDYAPLAEITGRSSLAPEALNCAAP
                     DTGNMEVLTMFGTDEQKEQWLRPLLEGEIRSCFAMTEPDVASSDATNIGLRIETDGDD
                     YVLNGRKWWISGAARERCKIAIVMGQTDPDAARHLQQSQVLVPMDTPGLTMVRDLTVF
                     GYTDQEGHCELAFEDVRVPRSNLIGEQGGGFAIAQARLGPGRIHHCMRVIGVAERALE
                     AMCRRVTSRTAFGKPLSEQGVIQEWIADSRIDIEQARLLVLKTAWLMDTVGNKGARIE
                     VSAIKVAVPNMALRIIDRAIQAHGAGGIGPDFGLSSMYAHVRTLRLADGPDEVHRMQL
                     AKREIRKYEAN"
     gene            complement(3701..>4151)
                     /locus_tag="VFP02_01305"
     CDS             complement(3701..>4151)
                     /locus_tag="VFP02_01305"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9541687.1"
                     /translation="GVIPLGEVVELEIPPTMEYLDLARVVVSAAASVDPAFPPSRVSD
                     LRLAVSELCANAIESHQRADNDEPLTIRCDLDDDRVTIEIEDHGGGFDPDGLSPMPHA
                     TDPSRLERERGLGIPLVRSLADDVEFLVKDDGTTVRVVLFHPNGTGR"
BASE COUNT          623 a         1443 c         1462 g          623 t
ORIGIN      
        1 agcgggcggg gggctgggtc tcgtggccct tggcctcgat gcttcgcacg gccacctggt
       61 cgccctcgcc catgtcgggc aggatccgct cctggtcctc gaggtcgccc tcgggatcgt
      121 cggagccctc gacgtaggcc cgcaggaagc ccgggtggtg gatggtcttg ccggtggccg
      181 cgaactcggc gtcacggccg tcgctcgcca tggccccgag ccgcacctga acactctggc
      241 cccgggcgtc ggtcatctgg gaggcgacgg tgcggcgcca gaccagctcg tagagacgga
      301 actcgtcgcc ctgcagctcg cgctggacct cctcggggag gcggaagcgg tcgcccgcgg
      361 ggcggatcgc ctcgtgggcc tcctgagcgt tcttgacctt gcgggtgtac tgccgcgggg
      421 catcgggcag gtggtcccgg ccgtacttct ccaggatctg ggcccgggcg gcggccacgg
      481 cggtgtccga cagcgtcgtg ctgtccgtcc gcatgtaggt gatgtaaccg ttctcgtaga
      541 ggcgttgggc cacgctcatg gtccgggcgg acgagaagcg cagcttgcgc cccgcctcct
      601 gctggagggt cgaggtcatg aacggcgcgt agggcttgcg ggtgtagggc ttggactcca
      661 ccgaccggac ggtgacgtcg gcgcggtcga gggccgaggc caggccccgg gccccggcct
      721 cgtccagcag ggtcacgtcg ccctggagcc ggccgtcctc gtcgaagtcc ttgcccgtgg
      781 ccagccgggc cccgtcgacg gcgaccaggg tggcaccgaa gtcggcctgc ggctcgccgc
      841 cggcgaaccg gccgtcgaga tcccagtagg aggcgaccgt gaagcgcatc cgggcccgtt
      901 cccgctcgac cacgatgcgg gtggccacgc tctggacccg gcccgccgac aggccgctct
      961 gcaccttctt ccagaggacg gggctgacct cgtagccgta gaggcggtcc aggatgcggc
     1021 gggcctcctg ggcatcgacc aggcggcggt cgatctcacg gggctcgtcg atggcccgtt
     1081 ggatggcctc gggcgtgatc tcgtggaaga ccatgcgtcg caccggcatg cccgacgggg
     1141 ggttcagcac ctcgagcagg tgccaggcga tggcctcccc ctcacggtcc tcatcggtcg
     1201 cgagatagag ctcgctggcc gccttcatct tcttccggag gttggcgacc acgtccttct
     1261 tgcccttgtt caccacgtac aggggcttga agtcgttgtc ggtgtccacg ccgaggcggg
     1321 cccagggctc gcccttgtag gcggcgggca cgtcggcggc gttctgaggc aggtcgcgga
     1381 tgtggccgac cgacgaatcg acgtcgtacc ccgagccgag gtagcccgcg atggtcttcg
     1441 ccttcgccgg gctctcgacg atcaccagag ggttggccac ggcgcggagg gtaggggcaa
     1501 tgacacgctc tgtgtcaacc accccacccg gacgggcggg tgaacgacta gcgggagtgg
     1561 atgctcccgc cgtcgatcat gtgcgcctga ccgctgacct ggcgggcctt gtccgaggcc
     1621 aaccagacca cggcttcggc gatgtcctcg ggctggagca actccccgct cggcaccgag
     1681 gaggccatca gctggcgcag gccgtcgtcc tcgcccatga agctcaggag catcggcgtc
     1741 tcggtggggc caggaaggac ggcattgacc cggatgccct gtctggcgta ctcggaggcc
     1801 gccgtgcggg tcaggccgag gacgccgtgc ttggccgcga cgtagtgggg gaggccggcg
     1861 aagccgaaga cgcccgcgcc cgacgaggtg ttgacgatgg ccccgccgcc gccccgctcg
     1921 accatgtggg ccaactcgtg cttcatgcac aggaagacgc tcgtgaggtt caccgcgatc
     1981 atccggtccc aggtggcctt gtccagctcg tggaaggtgg tcatgtggcc ggtgatgccg
     2041 gcgttgttga aggcggtgtc caggcgaccg tggtcggcca cgatccgggc gatgaggtcg
     2101 acgacgacgt cctcctcggc caggtcggcg tcgatccatt cggccgtcac gccggcggct
     2161 tgcaccgcgg cgacggtctc cgaggcgtcc ttcacatcgg cgatcaccac tgtttcggca
     2221 ccgtcccggg cgaacatccg ggccgccgcc gccccgatcc cgccggcccc gcccgtcacc
     2281 agcaccactg ctccgtccac gctcatggcc gggaccctag atcggggtca ggagtaggtt
     2341 cgccggtcgt ggacttcgaa ccgagcgaca agtgccgtga gctgtccgag cgcctcgagg
     2401 cgttcatggc cgagaaggtg atccccgccg agcccgtcta cgccgagcag atggccgcct
     2461 cgggcgacgg ccaccaccac ccccaggtca tggaggacct caaggccgag gcccgggccc
     2521 gggggctgtg gaacctcttc ctcccccacg ccacggagtg gaccgagggc ctgtccaacc
     2581 tggattacgc ccccctggcc gagatcaccg gccgcagcag cctggctccc gaggccctga
     2641 actgcgccgc acccgacacc gggaacatgg aggtgctgac catgttcggc accgacgagc
     2701 agaaggaaca gtggctccgg cccctgctgg agggcgagat ccgctcctgc ttcgccatga
     2761 cggagccgga cgtggccagc tccgacgcca ccaacatcgg cctgcgcatc gagaccgacg
     2821 gtgacgacta cgtcctcaac ggccgcaagt ggtggatctc gggggccgcc cgggagcgct
     2881 gcaagatcgc catcgtcatg ggccagaccg atccggacgc cgcccgtcac ctccagcaga
     2941 gccaggtgct cgtacccatg gacacgccgg ggctgacgat ggtccgggac ctgaccgtct
     3001 tcggctacac ggaccaggag gggcactgcg agctggcctt cgaggacgtt cgggtccccc
     3061 ggtccaacct gatcggcgag cagggcggcg ggttcgccat cgcccaggcc cgactcgggc
     3121 ccggccgcat ccaccactgc atgcgggtga tcggcgtggc cgaacgggcc ctggaggcca
     3181 tgtgccgccg ggtcaccagc cgcaccgcgt tcggcaagcc gctgtcagag cagggcgtca
     3241 tccaggagtg gatcgccgac tcccgcatcg acatcgagca ggcccgcctc ctggtcctca
     3301 agacggcctg gctgatggac acggtgggca acaagggcgc ccgcatcgag gtgtcagcca
     3361 tcaaggtggc cgtccccaac atggctctgc gcatcatcga ccgggccatc caggcccacg
     3421 gcgccggcgg gatcgggccc gacttcggcc tctcgtccat gtacgcccac gtgcgcacgc
     3481 tgcgcctggc cgacggcccc gacgaggtcc atcggatgca gctggccaag cgcgagatcc
     3541 gcaagtacga agcgaactga acgcccaccg tcgcgtcaga cgcggcgacc gcgaccgatc
     3601 acgccgatca cggcgaggat gacgaggacc acgaggatga cgaggagcag gttcatcgcc
     3661 tccccttacc cgactgggag aggttcaccc gaatctctcg tcagcggccc gtcccattcg
     3721 ggtggaagag gacgacgcgg accgtggtgc cgtcgtcctt gaccaggaac tccacgtcgt
     3781 cggccagaga ccggaccaga gggatgccga ggccccgctc ccgctcgagt cgcgacggat
     3841 cggtggcgtg gggcatcggg gacagcccgt ccgggtcgaa gccgccgccg tgatcctcga
     3901 tctcgatggt gacccggtcg tcgtcgagat cacagcggat ggtcagcggc tcgtcgttgt
     3961 ccgcccgctg gtgggactcg atggcgttgg cgcacagctc ggacacggcc aggcgcagat
     4021 ccgagacgcg ggacgggggg aaggcgggat cgaccgatgc ggccgcgctc accacgacac
     4081 gggcgagatc gaggtactcc atggtgggcg ggatctcgag ctcgacgacc tcgcccaacg
     4141 ggatcacccc g
//
LOCUS       DASTFV010000072         5273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_238456, whole genome shotgun sequence.
ACCESSION   DASTFV010000072 DASTFV010000000
VERSION     DASTFV010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238456"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..778)
                     /locus_tag="VFP02_01310"
     CDS             complement(209..778)
                     /locus_tag="VFP02_01310"
                     /inference="COORDINATES: protein motif:HMM:NF017697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate hydrolase family protein"
                     /protein_id="HET9541688.1"
                     /translation="MLAELLAHAGVEELLELRSSVGFLALHGGSLERETDRIALEAAE
                     VAGASLYALCQPDDLRWHIPSVQMDPADSLALGRFMAHVDVVLSVHGYGRDGMFTTLL
                     LGGGDRSLAAHTAGHLRTGLPGYTVVDDLGAIPKPLRGLHPDNPVNRTEGGGVQIELP
                     PRIRLQGPNADPAARQALVEALAAAALAL"
     gene            complement(768..1661)
                     /locus_tag="VFP02_01315"
     CDS             complement(768..1661)
                     /locus_tag="VFP02_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HET9541689.1"
                     /translation="MIDAPLALAFTAGMVATVNPCGFAMLPAYLTFFLGLEGGAADED
                     ARAGVARALAVGAVVTLGFVAVFTTVGALISHLSLAVDEWFPWMTIVVGIAVLALGVG
                     LLFGFELRLALPHLDRGGRERTLPSMFVFGVSYAVASLSCTLPVFLSVVAGTFSRSNT
                     ASGIAMYVCFALGMGLVLTVLTLALALARQSLVHRLRSALPFINRIAGVLLVVAGAYF
                     TWYGIYELRIRDDPRTAAGPVDTVTGWSSDITTWIQDLGATRLGLVLGIVVCAALLYV
                     LLRPADTVTPADEDDEPARAG"
     gene            complement(1658..2254)
                     /locus_tag="VFP02_01320"
     CDS             complement(1658..2254)
                     /locus_tag="VFP02_01320"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9541690.1"
                     /translation="MAETRVRSPIGVALVAMVLAGLVTALVLSFVLEDDGRPEASVLT
                     LAPPPGNGTVPQQSDLRGEPAPDFAFEPLHGGADVDFAAFRAGRPTVLNFFYKDCPPC
                     VVEMPDLQDAYEAYGDRVNFLGLSYEESVEDGLDLAERTGVTYELGRDPAGEIITAYA
                     GIGLPTTVFIDADGTIVTTHTGRIHPDELQDELDGLLA"
     gene            complement(2301..3563)
                     /locus_tag="VFP02_01325"
     CDS             complement(2301..3563)
                     /locus_tag="VFP02_01325"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET9541691.1"
                     /translation="MTSTRRKGAAAVLMAALLIVGACSSDDSGDDTRPANETTGPDDT
                     TTTTAAEAAGVDAERVQGVVEALADDELEGRDNHTDGSVLAQDLLVDQLSQISEPLDG
                     DTLDGYGHTFSEGTNLIGQIPGSDRADEYVVLGAHYDHLGHDCRTDTPGDDICNGAAD
                     NASGVATVLEVGHLLAADSEPPSRTIILAFWDAEEDDLGGSRDYVTDPPVPLEDTVAY
                     LNWDIQGANLSPALADTTVVVGAETGGPGLVAATQAATESSPLSTLQLSLLFGQGRSD
                     HANFASAGVPIVFFTDANTPCYHTAQDDVSNLDFDKLDQQILTAEALTRDLASTEDVP
                     VYDPTAPPATYDDAVSMLGVVSAAEPDFGLFSPEAQDTAAQFLTDLNTMVDAGAEAFD
                     DAAVSTLLGGSIDIVEALSTGVCDGFLD"
     gene            complement(3612..4829)
                     /locus_tag="VFP02_01330"
     CDS             complement(3612..4829)
                     /locus_tag="VFP02_01330"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9541692.1"
                     /translation="MTMARRRWTAVVLVGAVLAGGACSSDESGDGTAAAGPDADRVRG
                     VVEALADDEMEGRDNGSDGSTLAQDFLVDQLGQFSDPLDGDSLEGYGHTFALGTNLIG
                     VIAGGDRAEEYVVLGAHYDHLGWGGCRTDTPGDMVCNGAGDDASGVATVLEIGRSLAA
                     DPEPPPRTVVLALWDAEEDGLAGSAAYVDDPPVPLEDTAVYLNWDIQGSNLSPALADS
                     TVMIGAETGGPNLIAAAQAATEDSSLQTLVLSLRFGGGRTSDHVTFAAAGVPTVFFTD
                     GTTPCYHTSHDDVSTLDFDKLGQQIHTGLALTRDLVTTDDPPEYDGTTPPATYDDAVS
                     MLDVLSAGQPDFERFSPDVQATAEQFLIDLQSMVDAGEQAFDDAAVSTLLGGSVDVVE
                     ALSSGECDGFLDP"
     gene            complement(4930..>5273)
                     /locus_tag="VFP02_01335"
     CDS             complement(4930..>5273)
                     /locus_tag="VFP02_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HET9541693.1"
                     /translation="IDEEPKSWEAKARCRDGSGALTHLFFSEELQDIARAKRICADCP
                     VIEPCLSGALERREPWGVWGGQLILNGRILATKRRRGRPPKHPRPEDQLPVIPIPEAL
                     RETVGAAVGAA"
BASE COUNT          760 a         1795 c         1979 g          739 t
ORIGIN      
        1 acgagccccc cgggctcgta gccgtaccag atcgggatgg tgagcggcgg gccgtcggct
       61 cgctcgatcg agatgacgcc gacgtggaca ccggcgagga actcctgccg ctcgtgctcg
      121 ctcatggtca aggacatggg cgaagggtgt cacgtcgccc gacacgtcgc gttcggagat
      181 cagctggcgg gggctgaaat cgcccctcct agagggccag tgccgcggcg gcgagggcct
      241 cgacgagggc ctgacgggcg gcggggtcgg cgttggggcc ctgcaggcgg atgcggggcg
      301 gcagctcgat ctgcacccca ccgccctcgg tccggttgac cgggttgtcg gggtggaggc
      361 cccgcagggg cttggggatc gcgcccaggt cgtccacgac cgtgtagccg ggcaggcctg
      421 tgcgcaggtg gcccgcggtg tgggcggcca gcgaccggtc gccgccgccc agcagcagtg
      481 tggtgaacat cccgtcccgg ccgtagccgt ggaccgacag gacgacgtcg acgtgggcca
      541 tgaaccggcc cagggcaagg ctgtcggccg ggtccatctg caccgagggg atgtgccagc
      601 gcaggtcgtc gggttggcac agggcgtaca gcgaggcgcc ggcgacctcc gccgcctcga
      661 gggcgatgcg gtcggtctcc ctctccagcg agccgccgtg cagggccagg aagccgaccg
      721 acgagcgcag ctcgaggagc tcctcgaccc cggcgtgggc caggagctca gccagcacgg
      781 gcgggctcat cgtcctcgtc ggcgggggtg acggtgtcgg cgggccgcag caggacgtag
      841 agcagagcgg cgcacacgac gatgccgagc accagaccga gacgggtcgc ccccaggtcc
      901 tggatccagg tcgtgatgtc ggagctccac ccggtgaccg tgtcgaccgg gccggccgcg
      961 gtccgcgggt cgtcccggat gcgtagctcg tagatgccgt accacgtgaa gtaggcgccg
     1021 gccacgacca ggagcacccc ggcgatccga ttgatgaacg gcagggccga gcgcagccgg
     1081 tggaccagcg actggcgggc cagggcgagg gccagggtga gcacggtgag gaccaggccc
     1141 atgcccaacg cgaagcagac gtacatggcg atgcccgaag ccgtgttgga acggctgaag
     1201 gtcccggcca ccacgctcag gaacacgggg agggtgcagg acagcgacgc cacggcgtag
     1261 gacacgccga acacgaacat cgagggcagt gtccgctccc gcccgccgcg gtcgagatgg
     1321 gggagggcca gccgtagctc gaagccgaag aggaggccca cgccgagggc gaggacggcg
     1381 atgccgacca cgatcgtcat ccatgggaac cactcgtcca ccgccagtga caggtgggag
     1441 atgagcgccc cgacggtggt gaacacggcg acgaagccca gggtcacgac cgcgcccacc
     1501 gccagggccc gggccacacc ggcccgggcg tcctcgtcgg ccgcaccccc ctcgagtccg
     1561 aggaagaagg tcaggtaggc cgggagcatg gcgaagccgc aggggttgac cgtggccacc
     1621 atgccggccg tgaaggcgag ggccagcggg gcgtcgatca tgccaacagc ccgtccagtt
     1681 cgtcctgcag ctcgtcgggg tggatccggc ccgtgtgggt cgtgacgatg gtcccgtcgg
     1741 cgtcgatgaa caccgtggtc ggcagcccga tcccggcgta ggcggtgatg atctcgccgg
     1801 cggggtcccg cccgagctcg taggtcaccc ccgtgcgctc ggccaggtcc agcccgtcct
     1861 cgaccgactc ctcgtaggag aggccgagga agttcacccg gtcgccgtag gcctcgtagg
     1921 cgtcctgcag gtcaggcatc tccacgacgc agggggggca gtccttgtag aagaagttga
     1981 gcacggtcgg tcgccccgcc cggaacgcgg cgaagtcgac gtcggccccg ccgtggagcg
     2041 gctcgaaggc gaaatcagga gcgggctcgc cccggaggtc ggactgctga gggaccgtgc
     2101 cgttgccggg aggcggggcc aaggtgagca ccgaggcctc cggccgcccg tcgtcctcca
     2161 gcacgaagct caggaccagc gcggtgacga ggccggccag caccatggcg accagagcca
     2221 caccgatcgg gctccgcacc cgcgtctcgg ccatggccgg ggacggtacc cgtggtcagc
     2281 gacgggcgac tcgcggctgg ctagtcgagg aacccgtcgc agacgccggt gctcagggcc
     2341 tccacgatgt cgatcgagcc gccgagcagc gtgctgacgg cggcatcgtc gaacgcctcg
     2401 gcgccggcgt cgaccatggt gttgaggtcg gtcaggaact gggccgcggt gtcctgggcc
     2461 tcggggctga agaggccgaa gtcgggctct gccgcgctga ccaccccgag catcgacacg
     2521 gcatcgtcgt aggtggccgg cggcgccgtg gggtcgtaga cgggcacgtc ctcggtggag
     2581 gccaggtccc gggtcagggc ctcggcggtc aggatctgct ggtccagttt gtcgaagtcc
     2641 aggttggaga cgtcgtcctg cgccgtgtgg tagcagggcg tgttggcgtc ggtgaagaag
     2701 acgatgggca cgccggcgct ggcgaagttg gcgtggtcgc tgcggccctg gccgaacagg
     2761 aggctcagct gaagggtcga caggggggac gactccgtgg cggcctgggt ggcggcgacg
     2821 aggccggggc caccggtctc ggccccgacc acgacggtcg tgtcggccag ggccgggctc
     2881 aggttcgccc cctggatgtc ccagttcagg taggccaccg tgtcctccag cgggacgggc
     2941 ggatcggtga cgtagtcccg cgagccgccc aggtcgtcct cctcggcgtc ccagaaggcc
     3001 aggatgatcg tgcgggacgg gggctcggag tcggcggcca gcaggtggcc gacctccagg
     3061 accgtcgcca ccccggacgc gttgtccgcc gcgccgttgc agatgtcgtc accgggggtg
     3121 tcggtccggc agtcgtggcc gaggtggtcg tagtgcgcgc cgagcacgac gtattcgtcg
     3181 gcccggtccg aacccgggat ctgcccgatg aggttggtgc cctcggagaa cgtgtggccg
     3241 tagccgtcga gggtgtcgcc gtcgaggggc tcgctgatct ggctcagctg gtcgacgagg
     3301 aggtcttggg ccagcaccga gccatcggtg tggttgtccc gcccctccag ctcgtcgtcc
     3361 gccaacgctt cgaccacacc ctggacccgc tcggcgtcga ctccggccgc ttcggccgcg
     3421 gtggtggtcg tcgtgtcgtc ggggcccgtc gtctcgttcg ccggccgggt gtcatcaccg
     3481 ctgtcgtcgg agctgcacgc cccgacgatc agcagggcgg ccatcagcac ggcggccgcc
     3541 cccttccgcc gggtgctggt catggcgtga acctaggcca cctgccggtc ctcgggcccc
     3601 gcacgcctcg tctacgggtc gaggaagccg tcgcactcgc cggagctcag ggcctcgacg
     3661 acgtccactg agccaccgag cagggtgctg acggccgcgt cgtcgaaggc ctgctccccg
     3721 gcgtcgacca tggactggag atcgatgagg aactgctctg ctgtggcctg cacatcgggc
     3781 gagaaccgct caaagtcggg ttggccggca ctcaacacgt cgagcatgga gacggcgtcg
     3841 tcgtaggtgg ccggtggcgt cgtgccgtcg tactccggcg ggtcgtcggt ggtcaccagg
     3901 tcccgggtca gggccaggcc ggtgtggatc tgctggccca gcttgtcgaa gtcgagggtg
     3961 gacacgtcgt cgtgggacgt gtggtagcag ggcgtggtgc cgtcggtgaa gaacaccgtc
     4021 ggtacgcctg cggccgcgaa ggtgacgtgg tcgctggtgc gcccaccgcc gaaccgcagg
     4081 ctcaggacga gggtctgcaa cgacgagtcc tcggtcgcgg cctgggcggc ggcgatcagg
     4141 ttcggaccgc ccgtttccgc cccgatcatc acggtgctgt cggccagtgc cgggctcagg
     4201 ttcgagccct ggatgtccca gttgaggtag accgcggtgt cctcgagggg caccggcgga
     4261 tcgtcgacat aggccgcgga gccggcgagg ccgtcctcct ccgcgtccca cagggcgagg
     4321 accacggtcc gcggcggcgg ctccgggtcc gccgccagcg aacggccgat ctcgaggact
     4381 gtggccacgc cgctggcgtc gtcgcccgcc ccgttgcaga ccatgtcgcc cggcgtgtcg
     4441 gtccggcaac ccccccagcc gaggtggtcg tagtgggcgc ccaggaccac gtactcctcg
     4501 gcccggtcgc cgccagcgat caccccgatg aggttggtgc ccagggcgaa ggtgtggccg
     4561 tacccctcga ggctgtcgcc gtccaagggg tcgctgaact ggcccagctg gtcgacgagg
     4621 aagtcctggg ccagggtcga gccgtccgac ccgttgtccc gaccctccat ctcgtcatcg
     4681 gccaaggcct cgaccacgcc cctgacccgg tcggcatccg ggccagccgc cgccgtgccg
     4741 tcgccgcttt catcggagct gcaggccccg cccgccagca cggcccccac cagcactacc
     4801 gccgtccacc gccgtcgggc catggtcatg gcgggaacct aggccaccgg gcagacgggg
     4861 gttcgcagtc cgtcgtggtg gggggatccc cacgacggcc acgtcacccc cgtccgccgt
     4921 tggtggcggt caggcggccc cgacggcggc gccgacagtc tcccgcagcg cctccgggat
     4981 cgggatcacc ggaagctggt cctcgggccg cggatgcttg ggcggacggc cccggcgccg
     5041 cttggtggcc aggatccgac cgttgaggat cagctgcccg ccccagacgc cccacggctc
     5101 gcgccgctcg agcgcgccgg acaggcacgg ctcgatcacc gggcaatcgg cgcagatgcg
     5161 cttggcccgg gcgatgtcct ggagctcctc ggagaagaag aggtgggtca gggcgccact
     5221 gccgtcacgg cagcgggcct tggcctccca gctcttcggc tcctcgtcga tga
//
LOCUS       DASTFV010000073         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_244303, whole genome shotgun sequence.
ACCESSION   DASTFV010000073 DASTFV010000000
VERSION     DASTFV010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244303"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..567)
                     /locus_tag="VFP02_01340"
     CDS             complement(166..567)
                     /locus_tag="VFP02_01340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET9541694.1"
                     /translation="MAGLEVHLVTPEREVWSGEADFVTARGVDGDLGVLPGHAPLLAA
                     LAVGPVFIDADGSRIAVAVDGGFLHVAHEDDVTRVDILAEHAVLPDELAQDDAESHER
                     RAEELRSEDRLDEAREEEAKATVRRRVAENG"
     gene            complement(570..2018)
                     /gene="atpD"
                     /locus_tag="VFP02_01345"
     CDS             complement(570..2018)
                     /gene="atpD"
                     /locus_tag="VFP02_01345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET9541695.1"
                     /translation="MATVAYERDTGRSGGGTGRVVRIIGPVLDVEFAPDELPAINTAL
                     TVERTLDGEKTTITCEVAQHVGDGIVRAIAMSPTDGLIRGTPVHDTGSPMTMPVGENT
                     LGHVFNVLGQALDDPDFQAEKRWPIHRHAPSFDQLEPKTEMFVTGIKVIDLLEPYVAG
                     GKIGMFGGAGVGKTVVIQEMIHRVAEEHSGVSVFAGVGERTREGNDLFLEMSESGVID
                     KTALIFGQMDEPPGVRLRVGLAALTVAEYFRDEMGQDVLLFIDNIFRFVQAGSEVSTL
                     LGRMPSAVGYQPTLAGEMGVLQERITSTRGHSITSLQAIYVPADDITDPAPHTTFAHL
                     DATTVLAREIASLGIYPAVDPLTSTSRILDPRYVGDEHYRVARRVQEILQRYNDLQDI
                     IAILGVDELSEDDKVIVNRARRIQRFLSQPFYVAEQFTGVAGKTVPLDETIASFDALA
                     KGDYDHLPEQAFFNVGGIEEAEEKAKQLGGES"
BASE COUNT          272 a          717 c          736 g          320 t
ORIGIN      
        1 atggccgagg gctggtccaa cggcgccatc gccaggaccc tggtcgtcac cgaggccgcg
       61 gtggccaagc acatcggcag catcctggcc aagctcgacc tgccgccggc cgacgacgac
      121 caccgccgcg tccgggccgt gctcgcctac ctccgcgcct gagcctcagc cgttctcggc
      181 gacccgccgg cgcacggtcg ccttggcctc ctcctcgcgg gcctcgtcga ggcggtcctc
      241 ggagcgcagc tcctcggccc ggcgctcgtg cgactcggcg tcgtcctggg cgagctcgtc
      301 ggggagcacg gcgtgctcgg ccaggatgtc gacccgggtc acgtcgtcct cgtgggcgac
      361 gtggaggaag ccgccgtcca ccgcgaccgc gatgcggctg ccgtccgcgt cgatgaacac
      421 cgggccgacg gccagggcgg ccagcagcgg cgcgtggccg gggagcacgc cgaggtcgcc
      481 gtccaccccg cgggcggtga cgaagtcggc ctcgccggac cacacctccc gctcgggggt
      541 gaccaggtgg acctccaggc cggccacggc taggactccc cgccgagctg cttggccttc
      601 tcctcggcct cctcgatccc gccgacgttg aagaaggcct gctcgggcag gtggtcgtag
      661 tcgcccttgg ccagcgcgtc gaaggaggcg atggtctcgt ccagggggac cgtcttgccg
      721 gcgacgccgg tgaactgctc ggccacgtag aacggctggg acaggaaccg ctggatccgc
      781 cgggcccggt tgacgatcac cttgtcgtcc tcggacagct cgtcgacccc gaggatggcg
      841 atgatgtcct ggaggtcgtt gtagcgctgc aggatctcct ggacccgccg ggcgacccgg
      901 tagtgctcgt cgccgacata gcgcgggtcg aggatgcgcg aggtcgaggt cagcgggtcg
      961 accgccgggt agatgccgag cgatgcgatc tcgcgggcca gcaccgtggt cgcgtccagg
     1021 tgggcgaagg tggtgtgcgg ggccgggtcg gtgatgtcgt cggcgggcac gtagatcgcc
     1081 tgcagcgagg tgatggagtg gccgcgggtg gaggtgatcc gctcctggag cacgcccatc
     1141 tcgccggcca gggtcggctg gtagccgacc gcgctcggca tccggccgag cagggtcgag
     1201 acctcggagc cggcctgcac gaaccggaag atgttgtcga tgaacagcag cacgtcctgg
     1261 cccatctcgt cgcggaagta ctcggcgacg gtcagggcgg ccaggccgac ccgcagccgc
     1321 acccccggcg gctcgtccat ctggccgaag atcagcgccg tcttgtcgat gacgcccgac
     1381 tcgctcatct ccaggaacag gtcgttgccc tcgcgggtcc gctcccccac cccggcgaac
     1441 accgacacgc ccgagtgctc ctcggccacc cggtggatca tctcctggat gaccaccgtc
     1501 ttgccgaccc cggcgccgcc gaacatgccg atcttgccgc cggccacgta cggctccagc
     1561 aggtcgatga ccttgatgcc ggtcacgaac atctcggtct tgggctccag ctggtcgaag
     1621 ctgggggcgt ggcggtggat cggccagcgc ttctcggcct ggaagtcggg gtcgtcgagg
     1681 gcctggccga gcacgttgaa gacgtgcccg agggtgttct cgcccaccgg catggtcatg
     1741 ggcgagccgg tgtcgtgcac cggggtcccc ctgatcaggc cgtcggtggg cgacatggcg
     1801 atggcccgca cgatcccgtc gccaacgtgc tgggcgacct cgcaggtgat ggtcgtcttc
     1861 tcgccgtcga gggtccgctc gacggtcagg gcggtgttga tggcgggcag ctcgtcaggg
     1921 gcgaactcga cgtccagcac cggaccgatg atccggacga cccgcccggt gccgcctccg
     1981 ctgcggccgg tgtcccgctc gtatgcgacc gtggccatgt cgtgtcagct cctgttgctc
     2041 tggag
//
LOCUS       DASTFV010000074         3696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_245366, whole genome shotgun sequence.
ACCESSION   DASTFV010000074 DASTFV010000000
VERSION     DASTFV010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245366"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFP02_01350"
     CDS             complement(<1..229)
                     /locus_tag="VFP02_01350"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET9541696.1"
                     /translation="MTSTGLDQHIGDVGWLVASFVEGTADVADAIVVSSDGLLLAISP
                     QLDRGAADKLAAIVSGLRSLSEGVSSVTRRGG"
     gene            complement(226..2226)
                     /locus_tag="VFP02_01355"
     CDS             complement(226..2226)
                     /locus_tag="VFP02_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9541697.1"
                     /translation="MTKLKIRTRLLLALAIPLLALGGLGVRYLTTINQVKVDGRLYDQ
                     VTGPRAVVADVALLAENQSRALIDAEQLLRETSSSRIDGLLADLQVREDYYLAGVEAW
                     NGARVAEQPELAAMLDEAYGPSVEFYRVIDEELVPAVQAEDEDAMASAVAAVGRAYAV
                     QRAAADQVLDAAQVQATASEANAAAYLDDRLRQDQLIAAALVLIAVLTGALTVRSITR
                     PLRQLEQDLPRMANDLRRMDLAVERPEISHLAVDTHDELALATEAFTSVVETSVDLAV
                     EQARVRHNQAETLQHLGRRNQNLLRRMMAIINDLEHGERDAEALQELFRLDHIATRMR
                     RNAESLLVLAGSEQPRRWSEPVSVVDVVRSAASEIEDYDRIDLSNIEPVVVQGASAAD
                     ISHLLAELLENATLYSPPNTRVTVTGRRVRTGYQLAVLDQGLGLDEPSLARANDRILD
                     AAASASDLTDSKLLGLHVVGRLADRHDTYVALTPNPAGGLIALVTLPRAIVALAPSSA
                     GMIPQTVGPLPPAAAPRTAIAAPLAIAAPPPPPPPPAPPAVAAPPMHATPPPPVSAVP
                     PPASYPPPVTQPVAGPLAPVGANDNGASGPVPTVAVAAGPAPSLRRRVRSAEEGGPAE
                     AAEVVHGPTRTPKDVRDNWSRLALGVRQARLENGTNHEEARQ"
     gene            2446..>3696
                     /locus_tag="VFP02_01360"
     CDS             2446..>3696
                     /locus_tag="VFP02_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET9541698.1"
                     /translation="MATDPNRWTLKTQEAVAHAVEAAKAQNNPEVTPDHLLAALLGQA
                     EGVVLPILAKVGRPPLELRNQVQESLARLPHSYGSEAQFDAEFRTVLDDADREREQLT
                     DEYLSTEHLLLALAERVGVTHDELLAALQEVRGSHRVTSQSPEDQFQALEKYGRDLTE
                     DARQGKLDPVIGRDDEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEGLARRIVEGDV
                     PESLRNRRLISLDLGSMVAGAKFRGEFEERLKAVLKEITDAEGEVITFIDELHTIVGA
                     GAAEGAMDAGNMIKPLLARGQLRMIGATTLDEYRKHIEKDAALERRFQPVTVNEPSVE
                     DTISILRGLRERYEVHHGVRITDGALVAAAVLSNRYITERFLPDKAIDLVDESASRLR
                     MEMDSMPAELDEIERRRMQLEIERE"
BASE COUNT          536 a         1251 c         1397 g          512 t
ORIGIN      
        1 acccgcctcg ccgggtcacc gacgacacac cctcggacag gctgcgcagc cctgagacga
       61 tggcggccag cttgtcggct gcgccgcgat cgagctgcgg ggagatggcg agcagcagcc
      121 cgtcggagga gacgacgatg gcgtcggcca catcggccgt gccttccacg aagctggcca
      181 ccagccagcc gacgtcaccg atgtgctggt cgagcccggt gctggtcatt gcctcgcctc
      241 ctcgtggttg gtgccgttct ccaagcgggc ctgccggaca cctagggcca gtcggctcca
      301 gttgtcccgc acgtccttcg gggtccgcgt aggcccgtgg acgacctcgg cggcctcggc
      361 gggaccaccc tcttccgcgg agcgcacccg ccgccgcagc gacggggcgg gcccggccgc
      421 gaccgcgacg gtgggcacgg ggccggacgc tccgttgtcg ttcgccccga ccggtgcgag
      481 ggggccggcg acgggttggg tcaccggcgg tgggtaggag gcgggcggcg gcaccgcgct
      541 caccggcgga ggtggtgttg cgtgcatcgg gggcgccgcg acggctgggg gtgccggggg
      601 tggcgggggt ggcggcggtg ccgcgatcgc caggggtgcc gcgatcgcgg tccgcggggc
      661 ggccgcagga ggcaacggcc caacggtctg cgggatcatc ccggcggagc tgggggccag
      721 ggccacgatc gcccggggga gggtgaccag cgcgatgaga ccgccggcgg ggttgggcgt
      781 caacgccacg tacgtgtcgt ggcggtcggc cagtcggccc accacgtgca gcccgaggag
      841 cttcgagtcg gtgaggtcgg aggcgctggc ggcggcgtcg aggatccggt cgttggcccg
      901 ggccaggctg ggctcgtcga ggccgaggcc ctggtcgagg accgccagct ggtagcccgt
      961 ccgcacccgt cgccccgtca cggtcacccg ggtgttcggg ggcgagtaca gcgtggcgtt
     1021 ctcgagcagc tcggccagga ggtgggagat gtcggccgcc gaggcccctt gcaccacgac
     1081 gggttcgatg ttgctcaggt cgatccggtc gtagtcctcg atctccgacg ccgccgagcg
     1141 gacgacgtcg acaaccgaca ccggctcgct ccaccggcgg ggctgctcgc tgccggcgag
     1201 cacgaggagg ctctcggcgt tgcgccgcat gcgggtggcg atgtgatcca ggcggaagag
     1261 ctcctgaagg gcctcggcat cgcgttcgcc gtgctcgagg tcgttgatga tcgccatcat
     1321 ccggcgcagc aggttctggt tgcggcggcc caggtgctgg agggtctcgg cctggttgtg
     1381 gcggacgcgg gcctgctcga cggcgaggtc gacggaggtc tccacgacgg acgtgaacgc
     1441 ctcggtcgcc agcgccagct cgtcgtgggt gtcgacggcg aggtggctga tctcgggccg
     1501 ctcgacggcg aggtccatcc gccggaggtc gttcgccatc ctcggcaggt cctgctcgag
     1561 ctggcggagg ggtcgggtga tcgagcggac ggtgagcgca ccggtcagca cggcgatcag
     1621 gacgagggcg gcggcgatga gctggtcctg gcgcaggcgg tcgtccaggt aggcggcggc
     1681 gtttgcctcg ctcgcggtgg cctggacctg ggcggcgtcg agaacctggt cggcggcggc
     1741 ccgctgcacg gcgtaggccc gtccgacggc ggcgacggcg gacgccatgg cgtcctcgtc
     1801 ctcggcctgc acggcgggga cgagctcctc gtcgatgacg cggtagaact cgaccgacgg
     1861 gccgtaggcc tcgtcgagca tcgcagccag ctcgggctgc tcggcgacgc gggccccgtt
     1921 ccaggcctcc accccggcca ggtagtagtc ctcgcggacc tgcaggtcgg ccaggaggcc
     1981 gtcgatccgt gacgacgagg tctcgcggag cagctgctcg gcgtcgatca gggcccggga
     2041 ctggttctcg gccagcaggg ccacgtcagc cacgacggca cgggggccgg tgacctggtc
     2101 atagaggcgt ccgtcgacct tcacctggtt gatggtcgtc aggtagcgca cgcccaagcc
     2161 gccgagcgcg agcaacggga tggccagcgc cagcaggaga cgcgtgcgga tcttgagttt
     2221 cgtcatggcc accatccctg gaccccgggc cggcggcagc ccgagccctc ttgcttcaga
     2281 tatcggacgc ggtcacccgg acttgaaccc cggggccggg cggagttcgg cgcgaagccg
     2341 tcgccagccg cggccgacgt ctcggcgtcc tcggccgacc caggcgcaga cagaaaactt
     2401 gagtccttta ttgtcaagtt tgggtggtcg ccgtagactg ccacgatggc gacggacccg
     2461 aaccggtgga cgctgaagac gcaggaggcg gtggcccacg ccgtcgaggc ggccaaggcc
     2521 cagaacaacc ccgaggtcac acccgaccac ctgctcgccg ccctcctggg ccaggccgag
     2581 ggcgtggtcc tgcccatcct ggccaaggtc ggccgtccgc cgctggagct gcgcaaccag
     2641 gtgcaggagt ccctggcccg gctgccccac agctatggct cggaggcgca gttcgacgcc
     2701 gagttccgga cggtgctgga cgacgccgac cgcgagcggg agcagctcac cgacgagtac
     2761 ctctccaccg agcacctgtt gctggcgttg gccgagcgcg tcggggtcac ccacgacgag
     2821 ttgctggccg ccctccagga ggtccggggc agccaccggg tgaccagcca gagcccggag
     2881 gaccagttcc aggccctgga gaagtacggc cgggacctga ccgaggacgc ccgccagggc
     2941 aagctcgacc ccgtcatcgg tcgggacgac gagatccggc gggtcgtgca ggtcctgtcc
     3001 cgccggacca agaacaaccc ggtcctcatc ggggagccgg gcgtcggcaa gacggcgatc
     3061 gtggagggcc tggcccggcg catcgtcgag ggcgacgtgc ccgagagcct gcgcaaccgc
     3121 cgactgatca gcctcgacct gggcagcatg gtcgccggcg ccaagttccg gggcgagttc
     3181 gaggagcggt tgaaggcggt cctcaaggag atcaccgacg ccgagggcga ggtcatcacc
     3241 ttcatcgacg agctccacac catcgtgggc gccggcgcgg ccgaaggggc catggacgcc
     3301 gggaacatga tcaagccgct cctggcccgg ggccagctcc gcatgatcgg ggcgaccacc
     3361 ctcgacgagt accgcaagca catcgagaag gatgctgcgc tcgagcgccg cttccagccg
     3421 gtcacggtga acgagccgag cgtcgaggac acgatcagca tcctccgcgg gctccgcgag
     3481 cgctatgagg tccatcacgg cgtccggatc accgacggcg cgctcgtcgc cgcggcggtg
     3541 ctctccaacc gctatatcac cgagcgcttc ctgccggaca aggcgatcga cctcgtggac
     3601 gagtccgcat cccgcctgcg gatggagatg gactcgatgc ccgcggagct cgacgagatc
     3661 gagcgacgcc ggatgcagct cgagatcgag cgtgag
//
LOCUS       DASTFV010000075         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_247982, whole genome shotgun sequence.
ACCESSION   DASTFV010000075 DASTFV010000000
VERSION     DASTFV010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247982"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..435)
                     /locus_tag="VFP02_01365"
     CDS             complement(61..435)
                     /locus_tag="VFP02_01365"
                     /inference="COORDINATES: protein motif:HMM:NF019473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HET9541699.1"
                     /translation="MAEPIDVVREFCDAWGTPAAIDAALALLSDDCTYHNIPLEPVHG
                     IDEIRATIQGFSAGVEKVEFETLAIAANGSTVLTERIDTFFFPGKAIALPVMGTFEVN
                     ADGKISAWRDYFDMNQFLTQMG"
     gene            533..1705
                     /locus_tag="VFP02_01370"
     CDS             533..1705
                     /locus_tag="VFP02_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET9541700.1"
                     /translation="MTGAATAWGASGLAGLTGRADGPALAPGERTVDAVIALVADAPV
                     ADPLALLGERAALAGLTRAGSTSCGGSARLLPTADGWLAVNLARPEDIDAVPAWLALT
                     AAPSDPWTTVTEGVRTRSAEELVERAALLGLAVAAVGEVGPRIDTVARRRIRTGPPLV
                     TAPLVVDLSSLWAGPLCSHLLDLGGARVVKVESTARPDGGRSDDSGFFDLLNHGKASV
                     ALDLPSAEGVARLRSLLQAADVVIEGSRPRVLRQWGLDADALLADAAGPRVWVSITGH
                     GRDDAEGLRVAFGDDGAAAGGLVGRDDAGPCFLADAVADPLTGIAAASAVTTALAEGG
                     SWLLDAALAQVAASVADPQRTSRPVTGPVAPPRARPVPGPARPLGADTAAVLAELP"
     gene            1702..>2323
                     /locus_tag="VFP02_01375"
     CDS             1702..>2323
                     /locus_tag="VFP02_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541701.1"
                     /translation="MSALLIRGGEVDGRRADVLVDDGRIVAVGADLRPSGSPDTWDVD
                     GGAVLPGLHDHHLHLLAMAAAAGSLDLTDPAGGIEEAVRRAAADVAPGGWLRAVGHHA
                     AGGDVDRARLDDWAGPVPVRVQDASGALWILNSAALAAVAAAGIEVGADAATSGWILR
                     EDAALAAVWPAPALDLAAVGHGLAARGVTGVTDATPFTDPGGPTLLA"
BASE COUNT          262 a          859 c          891 g          311 t
ORIGIN      
        1 catcactccc gcaggcggcg atcagcagga gggccacggc caggggtacg aggcggcgca
       61 tcaacccatc tgggtgagga actggttcat gtcgaagtag tcgcgccagg cgctgatctt
      121 gccgtcggcg ttcacctcga aggtccccat cacgggcagg gcgatggcct tgccggggaa
      181 gaagaaggtg tcgatgcgct cggtgagcac ggtcgagccg ttcgcggcga tggccagcgt
      241 ctcgaactcg accttttcca ccccggccga gaagccctgg atggtggccc ggatctcgtc
      301 gatcccatgg accggctcga gcgggatgtt gtggtacgtg cagtcgtcgg acagcagggc
      361 caacgccgcg tcgatcgctg ccggggtccc ccaggcgtca cagaactccc gcaccacgtc
      421 gatcggctcg gccacgtctc cccctcggtt cgctgagcct gcgaccgtat ccgcgtcagc
      481 cgagagcggc gaaggccggc ctgctctgga cgccgtcgct accgtccgga cggtgacggg
      541 agcggccacg gcgtgggggg cgagcgggct cgccgggttg accgggcgag ccgacggtcc
      601 ggccctggcc ccgggcgaga ggacggtcga cgcggtcatc gccctggtgg ccgacgcgcc
      661 ggttgccgac ccgttggcct tgctcggcga gcgggccgcg ttggccggcc tgacccgcgc
      721 gggctcgacc agttgcggcg ggtcggcccg gttgctgccg acggccgacg ggtggctcgc
      781 cgtcaacctg gcccggcccg aggacatcga cgccgtgccc gcctggctgg ccttgaccgc
      841 cgcgccgtcc gacccgtgga ccaccgtgac cgagggggtg cgcacccggt cggcggaaga
      901 gctggtcgag cgggccgctc tcctcgggct ggccgtggcc gcggtcggcg aggtcgggcc
      961 ccggatcgac accgtcgccc gccgccggat ccggaccggg cccccgctgg tgacggcgcc
     1021 cctcgtggtg gatctcagct cgttgtgggc cgggccactg tgcagccacc tcctcgatct
     1081 cggtggcgcc cgggtggtga aggtggagtc gaccgcccgg ccggacggtg gtcgatcgga
     1141 cgactcgggg ttcttcgacc tgctcaacca cggaaaggca tcggtggccc tcgacctccc
     1201 gtcggccgag ggcgtggccc gactgcgcag cctcctgcag gcggccgacg tggtgatcga
     1261 gggctcccgc cctcgggtgc tgcggcagtg gggcctcgac gcggacgccc tactcgccga
     1321 cgctgccggc ccgcgggtct gggtgtcgat caccggccac gggcgggatg acgcagaggg
     1381 cctacgggtc gccttcggcg acgatggggc ggcggcgggg ggactggtcg ggcgcgatga
     1441 cgccggcccg tgcttcctgg ccgacgcggt ggccgatccc ctcaccggca tcgccgcggc
     1501 cagcgcggtg acgacggccc tggccgaagg gggaagctgg ctgctcgacg cggcgctggc
     1561 gcaggtcgcg gcctcggtgg ccgatccgca acggaccagc cggcccgtca ccggcccggt
     1621 ggccccgccc cgggcccgcc ccgtccccgg cccggcccga ccgctcggcg ccgacaccgc
     1681 agccgtcctg gccgagctcc cgtgagcgcg ctcctcatac gcggcggtga ggtcgacggg
     1741 cgacgggccg acgtgctggt cgacgacggc cggatcgtcg ccgtcggcgc cgacctgcgt
     1801 ccgagcggct cgcccgacac ctgggacgtc gacggcggag cggtgctgcc cggtctccac
     1861 gaccaccacc tccacctcct ggccatggcc gcggccgcgg gatcgctgga cctgaccgac
     1921 cccgcgggcg ggatcgaaga ggccgtgcgc cgggccgcgg ccgatgtcgc gccgggcggg
     1981 tggctccggg ccgtgggcca tcacgccgcg ggcggggacg tcgaccgagc ccggctcgac
     2041 gactgggccg gcccggtgcc cgtgcgggtc caggacgctt cgggggccct ctggatcctc
     2101 aactcggccg ctctggccgc ggtcgccgcg gcaggcatcg aggtcggcgc cgatgcggcc
     2161 acgtcgggct ggatcctccg tgaggacgcg gcgttggccg cggtgtggcc cgccccggcc
     2221 ctcgatctgg ccgccgtcgg gcacggcctc gcggctcggg gcgtcaccgg ggtcaccgac
     2281 gccacgccgt tcaccgaccc gggtggcccg accctcctgg ccg
//
LOCUS       DASTFV010000076         3217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_249229, whole genome shotgun sequence.
ACCESSION   DASTFV010000076 DASTFV010000000
VERSION     DASTFV010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249229"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /gene="argS"
                     /locus_tag="VFP02_01380"
     CDS             <1..992
                     /gene="argS"
                     /locus_tag="VFP02_01380"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET9541702.1"
                     /translation="RQDQIDTLAGLGVAFDVWFSESSLAHSGAIEQSLADLLARDAPD
                     KPEDVDEWKVAYEADGATWLRSTVFGDDKDRVLVKTDGEYTYVLPDIAYHRDKFARGF
                     ELLINVWGADHHGYVSRLKNAVQALGHDPDELEIVITQLVSLFKNGEPVRLSKRTGQI
                     VTIDEIVDEIGADSARITFLLQSMDSPQTVDLAEVASQAMENPVFYVQYAHARIRSIG
                     RKVAELGVERAPLADVDLGLLTHERELELLRSLSELPEVVAGATLGRAPHQVTTWVRE
                     LAGRFHGFYHDCYVVADSVDPALTQARLWLVEAVRIGLAIGLDLLGASAPDEM"
     gene            1031..2257
                     /gene="lysA"
                     /locus_tag="VFP02_01385"
     CDS             1031..2257
                     /gene="lysA"
                     /locus_tag="VFP02_01385"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET9541703.1"
                     /translation="MGDAGRLSIGGCDLLDLAAEIGTPVFVYDETHLRARCAEAVAAF
                     GEGVAYAAKAFLCRAMAQLAHEEGLYLDVATGGELHVALAAGVPPDRLVLHGNNKSEA
                     ELARALDAGVGRIVVDSFDEVDRLERLAPDAPSRPQVLVRVTPGVEAHTHEYVRTGQD
                     DSKFGFGLRSGAAGEAVERIGAMRSVDLIGLHAHIGSQVFVVDSFFQAVEVLAPFVNA
                     QGLPELSIGGGLGVAYVEGESAPTITEWGGAVRAACVDAGITARVSAEPGRAIAAAAA
                     VTLYTVGTIKDLRGIRTYVAVDGGMSDNPRPVLYGSGYETFLPRATDAVRDRPVRVVG
                     KHCESGDLLVAEGRVPEDLAVGDILATPVTGAYGHSMGSNYNKVPRPPVVFVKDGDAR
                     LVVRRETDDDLLRLDL"
     gene            complement(2263..2754)
                     /locus_tag="VFP02_01390"
     CDS             complement(2263..2754)
                     /locus_tag="VFP02_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9541704.1"
                     /translation="MSELPGGWTPEAVLGGRADRVVMRARRPGQAPVVVKATPPGAGW
                     RSRAALRREARLLEAAQGEGVVHLLDVLDRQGRTVLVLAFVPAGALADRTAPDPDLVV
                     AHLTTAVERLHRLGLGHGALRPEHILLEADGRPVLCGFGAAHRSHDRDPDATALAALI
                     RAV"
     gene            complement(2747..>3217)
                     /locus_tag="VFP02_01395"
     CDS             complement(2747..>3217)
                     /locus_tag="VFP02_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541705.1"
                     /translation="FVISGPPQSGRSTAMARFLTAPDRPLTVYRAGGDAERFRAEVRA
                     WLDGPRPRLFAVDDAETLTDPEGVLRDLVTRRHPDARIVVGVRADAWRSAYGSWLSEL
                     RPAGHGLALAPDPVHDAESWTLALPPLGPSPPPGRGVLVDDGQAAVIQVALADE"
BASE COUNT          441 a         1170 c         1182 g          424 t
ORIGIN      
        1 cccgccagga ccagatcgac accctcgccg ggctcggggt ggccttcgac gtctggttct
       61 cggagtcgtc actggcccat tcgggggcga tcgagcagag cctggccgac ctcctggccc
      121 gggacgcccc ggacaagccc gaggacgtcg acgagtggaa ggtggcctac gaggccgacg
      181 gtgccacctg gctgcggtcg accgtcttcg gcgacgacaa ggaccgggtc ctcgtcaaga
      241 ccgacggcga gtacacctac gtgctccccg acatcgccta ccaccgggac aagttcgccc
      301 ggggtttcga gctcctcatc aacgtgtggg gggccgacca ccacggctac gtctcgcggc
      361 tcaagaacgc ggtgcaggcc ctgggccacg atcccgacga gctcgagatc gtgatcaccc
      421 agctggtcag cctgttcaag aacggcgagc cggtgcggct gtcgaagcgg accggccaga
      481 tcgtcaccat cgacgagatc gtggacgaga tcggcgccga cagcgcccgc atcaccttcc
      541 tgctccagtc gatggacagc ccgcagaccg tcgacctggc cgaggtggcc agtcaggcca
      601 tggagaaccc ggtcttctac gtgcagtacg cgcacgcccg gatccggtcc atcgggcgca
      661 aggtggccga gctgggggtc gagcgggccc cgctggctga cgtcgacctg ggcctgctca
      721 cccacgagcg ggagctggag ctgctgcgct cgctctccga gctgcccgag gtggtggccg
      781 gggccaccct gggccgggcg ccccaccagg tcacgacctg ggtccgggaa ctggccggga
      841 ggttccacgg cttctaccac gactgctacg tggtggccga cagcgtcgat cccgccctga
      901 cccaggcccg cctgtggctg gtcgaggccg tacggatcgg cctggccatc gggctggacc
      961 tgctgggggc cagcgcgccc gacgagatgt gaccccgctc ccgcgggggc tcctgcctga
     1021 caccgcgacg gtgggcgacg ccggccgcct gagcatcggg ggctgtgacc tcctcgacct
     1081 ggccgccgag atcggaaccc cggtattcgt ctacgacgag acccacctgc gggcccgctg
     1141 cgcagaggca gtggccgcct tcggcgaggg cgtggcctat gcggccaagg cctttctctg
     1201 ccgggcgatg gcccagttgg cccatgagga gggcctgtac ctcgacgtgg ccacgggggg
     1261 ggagctgcac gtggccctgg ctgccggcgt cccgcccgat cgcctggtcc tgcacggcaa
     1321 caacaagtcc gaggccgaac tggcccgggc cctcgacgcc ggggtcggcc gcatcgtcgt
     1381 cgactccttc gatgaagtcg accggctcga gcgcctcgct cctgatgccc cgagccggcc
     1441 ccaggtcctg gtccgggtca ccccgggcgt cgaggcccac acccacgagt acgtgcgcac
     1501 gggccaggac gactccaagt tcggcttcgg cctccggtcc ggcgccgccg gcgaggccgt
     1561 cgagcgcatc ggtgccatgc gctcggtcga cctgatcggg ctgcacgccc acatcggcag
     1621 ccaggtcttc gtcgtcgact ccttcttcca ggccgtcgag gtgctggccc ccttcgtcaa
     1681 cgcccaaggc ctgcccgagc tgtcgatagg cggcggcctc ggcgtggcct acgtcgaggg
     1741 cgagtcggcc ccgacgatca ccgagtgggg cggggcggtg cgggccgcgt gcgtcgacgc
     1801 cggcatcacc gcccgggtgt cggccgagcc cggtcgggcg atcgccgcgg ccgccgccgt
     1861 caccctctac acggtcggca ccatcaagga cctgcgcggc atccgcacct acgtcgccgt
     1921 cgacggtggc atgagcgaca acccccgccc cgtgctctac ggcagcggct acgagacctt
     1981 cctgccccgg gccaccgacg ccgtgcggga ccgtccggtg cgggtcgtgg gcaagcactg
     2041 cgagtcgggc gacctgctgg tggccgaggg ccgggtcccc gaggacctcg ccgtcggcga
     2101 catcctggcc acgccggtca ccggcgcgta cggccactcc atgggctcca actacaacaa
     2161 ggtccctcgc ccgccggtcg tgttcgtcaa ggacggcgat gcccgtctcg tcgttcgccg
     2221 cgagaccgac gacgacctcc tccgcctcga cctctgacgg cgtcagacgg cgcggatgag
     2281 cgcggcaagg gcggtggcgt cggggtcgcg gtcgtgactg cggtgggcgg cgccgaagcc
     2341 gcacaggacc gggcgcccgt cggcctcgag gaggatgtgc tcggggcgga gagcgccatg
     2401 gcccaggccg aggcggtgga ggcgctcgac cgcggtcgtc aggtgggcga cgacgaggtc
     2461 cgggtcgggg gcggtgcggt cggccagcgc gccggccggg acgaaggcga gcacgagcac
     2521 cgttcggccc tggcggtcca ggacgtcgag gaggtggact acgccctcgc cctgcgccgc
     2581 ttccagcagc cgggcctccc ggcggagggc ggcccgtgac ctccagccgg cgccgggtgg
     2641 ggtggccttg accacgacgg gcgcctggcc cgggcggcgc gcccgcatca cgacccggtc
     2701 cgctcgaccc ccgaggacgg cctcgggggt ccaaccgcca gggagctcac tcatcggcga
     2761 gggcgacctg gatgaccgcc gcctgaccat cgtccaccag cacgccccgc ccgggtggcg
     2821 ggctcggccc gagcggggga agggcgaggg tccacgattc ggcgtcgtgc accgggtcgg
     2881 gggccagggc caggccgtga ccggccggac ggagctcgga caaccaggag ccgtaggccg
     2941 agcgccaggc gtccgcccgc acgccgacca cgatccgggc gtcggggtga cggcgggtga
     3001 cgaggtcccg gagcacgccc tcggggtcgg tcagggtctc ggcgtcgtcc acggcgaaca
     3061 gtcggggtcg aggcccatcg agccaggctc ggacctcggc ccggaaccgc tcggcgtcgc
     3121 cgccggctcg gtacaccgtc agcgggcggt cgggcgcggt caggaaccgg gccatcgcgg
     3181 tggagcgccc ggactgcggt gggccactga tcacgaa
//
LOCUS       DASTFV010000077         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_250483, whole genome shotgun sequence.
ACCESSION   DASTFV010000077 DASTFV010000000
VERSION     DASTFV010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250483"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VFP02_01400"
     CDS             <1..1213
                     /locus_tag="VFP02_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9541706.1"
                     /translation="PKAVFVADVTEATTRGVWGDKGGFHHTAEVWDLAAAAAGDPAPV
                     TIEPMSDLAALPYSSGTTGAPKGVMLSHHNITSNVQQIRGTDLIPDGSVMIDFLPFFH
                     IYGLTVLMLCGLASGSKQVVLPGFDPQRFVDLTQSHRATNLFLVPPAMLALANMGDQL
                     GADLSSVRYVLSGAAPLPIDVARRVAEHYDVEVVQGYGMTEASPVTHISLLGHDKPGT
                     VGPPVADTRQKVVDLATGEELGIGETGELLIQGPQVMAGYYQRPDATEETLLTDADGV
                     WLRTGDIVTVDEDGFVTIRDRAKEMIKYKGYQIAPAELEAVLIENPDIADAAVIPKDS
                     GNGMDEIPKAFVVLREGATLSADEVMAYVDERVAPYKKVREVAFVEAIPRNPSGKILR
                     RELKEKERASS"
     gene            1210..>2144
                     /locus_tag="VFP02_01405"
     CDS             1210..>2144
                     /locus_tag="VFP02_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9541707.1"
                     /translation="MSDLPALLHPFAKPSAPRDHFVTVVRGDGALVWDADGNEYVDGM
                     ASLWFANVGYGRAEVADAVAAQVRTLHAYHCFDPFTNGPADALAERLAALAPVDDPRI
                     FLVSSGSEAVDSAMKLAKAAHIRAGRPERTLIVSRSAGYHGVTYGGTSAQGIPDNRAG
                     FGELLPDVVNVAGDDIEAMAMLFAERGDEVAAVISEPVQGAGGVFPPPEGYLTQLRRL
                     CDDHGAFLILDEVICAFGRLGRWFGAEHYEVRPDIITFAKGVTSGYVPLGGVVLGEPV
                     HTALASDPTFILRHGHTYSGHATAAAAAMANLDVI"
BASE COUNT          339 a          795 c          693 g          317 t
ORIGIN      
        1 gcccaaggcc gtcttcgtcg ccgacgtcac cgaggccacc acccgcggcg tgtggggcga
       61 caagggcggc ttccaccaca cggccgaggt ctgggatctg gcggcggccg ccgccggaga
      121 ccccgccccg gtgaccatcg agccgatgag cgacctggcc gccctcccct actcctccgg
      181 gaccactggt gctcccaagg gagtcatgct cagccaccac aacatcacga gcaacgtgca
      241 gcagatccgc gggaccgacc tcatcccgga cggctcggtg atgatcgact tcctgccgtt
      301 cttccacatc tacggcctga ccgtcctgat gctctgcggc ctggccagcg ggtccaagca
      361 ggtcgtcctg cccggcttcg acccccagcg cttcgtggac ctcacccaga gccaccgagc
      421 caccaacctc ttccttgtgc cgcccgccat gttggccttg gccaacatgg gtgaccagct
      481 cggcgccgac ctgagcagcg tgcgctacgt gctgtcgggg gcggctcccc tgcccatcga
      541 cgtggcccgc cgggtggcgg agcactacga cgtcgaggtc gtccagggct acggcatgac
      601 cgaggccagc ccggtgaccc acatcagcct gctcggccac gacaagccgg gcaccgtcgg
      661 gccgcccgtt gcggacacgc gccagaaggt ggtcgacctg gctaccgggg aggagctggg
      721 catcggcgag accggtgagc tgctcatcca gggcccgcag gtcatggccg gctactacca
      781 gcgccccgac gccaccgagg agaccctgct gaccgacgcc gacggcgtct ggttgcgcac
      841 cggcgacatc gtgaccgttg acgaggacgg cttcgtcacc atccgtgacc gggccaagga
      901 gatgatcaag tacaaggggt accagatcgc gccggccgag ttggaggccg tcctcatcga
      961 gaaccctgac atcgccgacg ccgccgtcat ccccaaggac agcggcaacg ggatggacga
     1021 gatcccgaag gccttcgtgg tccttcggga gggggcgacg ctgtcggccg acgaggtcat
     1081 ggcctacgtc gacgagcgag tcgcgcccta caagaaggtc cgcgaggtgg ccttcgtcga
     1141 ggccatcccg aggaatccga gcggcaagat cctgcgccgc gaactcaagg agaaggagcg
     1201 cgcgtcctca tgagtgacct tcctgccctg ttgcacccgt tcgccaagcc atcggcgccg
     1261 cgggaccact tcgtcaccgt cgtccggggc gacggagccc tcgtctggga cgccgacggc
     1321 aacgagtacg tcgacggcat ggccagcctg tggttcgcca acgtcggcta cggccgcgcc
     1381 gaggtggccg acgccgtcgc cgcccaggtc aggacgctgc acgcctacca ctgcttcgac
     1441 cccttcacca acggcccagc cgacgccctc gcggaacgcc ttgccgccct cgccccggtt
     1501 gacgacccac ggatcttcct ggtcagctcc ggctcggagg ccgtcgactc cgccatgaag
     1561 ctcgcgaagg ccgcccacat ccgggcgggt cgacccgagc ggacgctcat cgtcagccgg
     1621 tcggcgggct accacggcgt gacctacggc gggacctcag cccagggcat ccccgacaac
     1681 cgggccgggt tcggcgagct tctgcccgat gtggtgaacg tcgccggcga cgacatcgag
     1741 gccatggcca tgctcttcgc cgaacggggc gacgaggtgg ccgccgtcat cagcgagccg
     1801 gtccaggggg cggggggcgt gttcccccct cccgagggct acctgaccca actccgacgc
     1861 ttgtgcgacg accatggggc cttcctgatc ctggacgaag tcatctgtgc cttcgggcgc
     1921 ctcggtcgtt ggttcggggc cgagcactac gaggtccgtc ccgacatcat cacgttcgcc
     1981 aagggggtca cctccggcta cgtgcccctc ggcggtgtcg tgctgggcga gcccgtgcac
     2041 accgcgctgg cctccgaccc gacgttcatc ctccgtcacg gtcacaccta ctcgggtcat
     2101 gccaccgcgg ccgccgcggc catggccaac ctcgacgtca tcga
//
LOCUS       DASTFV010000078         2947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_250986, whole genome shotgun sequence.
ACCESSION   DASTFV010000078 DASTFV010000000
VERSION     DASTFV010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250986"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..392)
                     /locus_tag="VFP02_01410"
     CDS             complement(72..392)
                     /locus_tag="VFP02_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541708.1"
                     /translation="MAKDPDCDLCEAAKITHWFHEDDVCWIAECELCSTPMVVWKDHG
                     TDPPPDAKGHMHDELGRVALATIGEFYVDDNMRNIPDHYHAHARPKGGFFGHHLRKHW
                     IDAP"
     gene            419..847
                     /locus_tag="VFP02_01415"
     CDS             419..847
                     /locus_tag="VFP02_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9541709.1"
                     /translation="MTDWVRDRLEIDDLLTRYATAIDTMELDLLDEVFTGDADCDYEV
                     VGGFRGDRAAFKDWLHEVMGFFEFHMHYVTNRTVTLDGDEATAVSYLYNPCVLKGGDG
                     KVLTEGGRYHDRIVRTPAGWRLAARREEPIWNAWPPGLRP"
     gene            929..2071
                     /locus_tag="VFP02_01420"
     CDS             929..2071
                     /locus_tag="VFP02_01420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9541710.1"
                     /translation="MEFGIFNSLYCPHQAVEAGPDPELVEHTRLMDEVAWTKTADRSG
                     FKYTWATEHHFLEEYSHLSANESFLAYLAGVTEDIHLGSGIINITPPVNHPARVAERV
                     AMIDHLSGGRFEFGTGRGSSTTEQAGFGITDPDLTKAMWAEALPQIVRMWTETDYSFD
                     GEFFSMPPRNVLPKPLTKPHPPLWVAAGNPGTFETAAKMGLGVLCFGISNPEALKPLI
                     DIYKTNIGDADPVGGFVNDNIMVTTQMLCLEDGQKAKEIATRMTTGYHTSSLFRYLDT
                     FPKPEGLPTWPELIPEPTLEQIEQSVERGLMAIGSPDEVEACVAKYEATGADQLVFGM
                     LSTTMPIDIAVEAVETFGRDLIPAFDKDPVHRTTAQREAQVSPQPA"
     gene            2089..>2947
                     /locus_tag="VFP02_01425"
     CDS             2089..>2947
                     /locus_tag="VFP02_01425"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET9541711.1"
                     /translation="MSTPAVRRVTEGLRFPEGPVALSDGSIVVCEIEAGRVTRVQPGG
                     AKTTVAVVGGGPNGAALGPDGALYVVNNGGFIWSDLGGMLIPLDLATGANEPPDFQGG
                     WVDRVDLDTGEVTTLYRECDGRRLCSPNDIVFDAEGGFWFTDLGKMRAHDLDRGGLFY
                     ATADGSTITQPVHGLLGANGVGLAPDGGTVYVAESHTGRLLAWTVEGPGRVAGRGGVR
                     HGGRCVVATPAGFDSLAVEADGHVVVAAISHGLCVVDPATGEHEFVEMPDPLVTNICF
                     TGDDDRTAYT"
BASE COUNT          466 a         1064 c          972 g          445 t
ORIGIN      
        1 gccgtggagc gggaggtgcc aggtcatctg cgggaccttg gtgatgcgga cggcgccgac
       61 ggtccacctg gtcacggggc gtcgatccag tgcttgcgca ggtggtggcc gaagaagccg
      121 cctttggggc gggcgtgggc gtggtagtgg tccgggatgt tgcgcatgtt gtcgtcgacg
      181 tagaactcgc cgatcgtggc cagcgccacc cggcccagct cgtcgtgcat gtggcccttg
      241 gcgtccggcg gcgggtcggt gccgtggtcc ttccagacca ccatcggtgt cgagcagagc
      301 tcgcactcgg cgatccagca cacgtcgtcc tcgtggaacc agtgggtgat cttggccgcc
      361 tcgcagaggt cgcagtccgg gtccttcgcc atatcggcca gaccctacgc tgccgcccat
      421 gaccgactgg gtgcgtgacc gactcgagat cgacgacctc ctgacccgct acgccacggc
      481 catcgacacc atggagctcg acctgctcga cgaggtgttc acgggcgacg ccgactgcga
      541 ctacgaggtc gtcgggggct tccggggcga tcgggccgcc ttcaaggact ggctccacga
      601 ggtgatgggg ttcttcgagt tccacatgca ctacgtgacc aaccgcaccg tcaccctgga
      661 cggcgacgag gcgaccgcgg tcagctacct ctacaacccc tgcgtcctga agggcggcga
      721 cggcaaggtc ctcaccgagg ggggccgcta ccacgaccgg atcgtgcgca cccccgccgg
      781 ctggcgcctc gccgcccggc gtgaagagcc gatctggaac gcctggcccc ccggcctccg
      841 gccgtaagag gctgcgctcc cttcgcagcg tcgggccgtg ctgggggcaa aactgaaacg
      901 tgttctagtt tgctgttagc gttcgggcat ggagttcggc atcttcaaca gcctctactg
      961 cccgcaccag gccgtggaag caggtcccga tccggagctc gtcgagcaca cccggctgat
     1021 ggacgaggtg gcctggacca agaccgcgga ccggtcgggc ttcaagtaca cgtgggccac
     1081 cgagcaccac ttcctcgagg agtactccca cctctcggcc aacgaatcct tcctcgccta
     1141 cctggccggc gtgaccgagg acatccacct cggcagcggg atcatcaaca tcaccccgcc
     1201 ggtcaaccac ccggcccgag tggccgagcg ggtggccatg atcgaccacc tctccggcgg
     1261 ccggttcgag ttcggcaccg gccggggctc gtcgaccacg gagcaggccg gcttcggcat
     1321 cacggatccg gacctgacca aggccatgtg ggccgaggcc ctgccccaga tcgtccgcat
     1381 gtggaccgag acggactact cgttcgacgg cgagttcttc tcgatgcccc cgcgcaacgt
     1441 tctgcccaag ccgctgacca agccccaccc acccctgtgg gtcgccgccg gcaaccccgg
     1501 caccttcgag accgcggcca agatgggcct gggcgtgctc tgcttcggca tctccaaccc
     1561 ggaggccctc aagcccctga tcgacatcta caagaccaac atcggcgacg ccgacccggt
     1621 cgggggcttc gtcaacgaca acatcatggt gacgacgcag atgctgtgcc tggaggacgg
     1681 gcagaaggcc aaggagatcg ccacccggat gaccaccggc taccacacgt ccagcctctt
     1741 ccggtatctc gacaccttcc ccaagcccga gggcctcccg acctggcccg agctcatccc
     1801 cgaaccgacg ctggaacaga tcgagcagtc ggtcgagcgg gggctcatgg ccatcggctc
     1861 gcccgacgag gtcgaggcct gcgtggccaa gtacgaggcg accggcgccg accagctcgt
     1921 cttcgggatg ctctcgacga cgatgcccat cgacatcgcc gtcgaagccg tggagacctt
     1981 cggtcgcgac ctgatccccg ccttcgacaa ggacccggtg caccgcacca cggcccagcg
     2041 cgaggctcag gtgagccctc agcccgccta agcggcgatg atgggccggt gagcacaccg
     2101 gcggtccgac gcgtcaccga gggcctgcgc ttccccgagg gccccgtggc cctgtccgac
     2161 gggtcgatcg tcgtgtgcga gatcgaggcg ggccgcgtga cccgcgtgca gcccggcggc
     2221 gccaagacca cggtcgccgt cgtcgggggc gggccgaacg gcgccgcgct cggtcccgac
     2281 ggcgcgctct acgtcgtcaa caacggcggc ttcatctgga gcgatctcgg cgggatgctc
     2341 atccctctcg acctggccac cggggccaac gagccaccgg acttccaggg cggctgggtc
     2401 gaccgggtcg acctcgacac cggtgaggtc accaccctct accgcgagtg tgacggcagg
     2461 cggctgtgca gccccaacga catcgtcttc gacgccgagg gcggcttctg gttcaccgac
     2521 ctcggcaaga tgcgggccca tgacctcgac cgcggcggcc tcttctacgc caccgcggac
     2581 ggctcgacga tcacccaacc cgttcacggc ctcctcgggg ccaacggcgt cggcctggct
     2641 cccgacggag gcaccgtgta cgtggcggag tcccacaccg ggcgcctcct ggcctggacc
     2701 gtcgaggggc cgggacgggt cgccgggcgc ggtggggtga ggcatggtgg acgctgcgtc
     2761 gtggccacgc ctgccgggtt cgactccctc gccgtggagg cggacggcca cgtcgtcgtg
     2821 gccgccatca gccacggact gtgcgtggtg gacccggcca cgggcgagca cgagttcgtc
     2881 gagatgcccg accccctcgt caccaacatc tgcttcaccg gtgacgacga ccgcaccgcc
     2941 tacacca
//
LOCUS       DASTFV010000079         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_269784, whole genome shotgun sequence.
ACCESSION   DASTFV010000079 DASTFV010000000
VERSION     DASTFV010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269784"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1809)
                     /locus_tag="VFP02_01430"
     CDS             complement(202..1809)
                     /locus_tag="VFP02_01430"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HET9541712.1"
                     /translation="MSVYVAEEFSSDEEDILRRYFTNLDRPVFALVNLPEVVKGALFA
                     RYSRSPKSLRRLFLDEFVVDLDITGDQTVDATMGLERAEELYQTVFYDYGDDSVAQLG
                     GVHLACEQASNILTKILEWGRLMAYLEQSTRYIAYDARIGGRFRYYRDPDVLQSNLGT
                     RYVGDMDRLFDTYAELVPTLGDFFRDNIPKDPADSDFIYRQSIRAKAFDALRGILPAA
                     SLSNVGIYGTGQGYEQLLLRMRAHPLPEARSYAGMMLTELRKVIPSFLRRVDVPDRGV
                     AWSNYLAENRMAMDEMAAKVFPTSGEMDAEPAVTLVDFDPDAEVKMVASMLYAHTDRP
                     ETEIEARVRSMSTDERLAVVRAYCGERSNRRHKPGRALERSFYRFDVLTDYGAFRDLQ
                     RHRMLTIEWQRLTPRHGFTRPESVDAAGQAARFDEAMERSEALFDDLEPAFPDQASYA
                     VALAYRIRFVMQINARAAMHMLELRTTPQGHPAYRWVAQQMHQRIADEAGHHAVAELM
                     RYVDHDPEPDLERLDAERRAEARRLTP"
     gene            complement(1887..>2919)
                     /locus_tag="VFP02_01435"
     CDS             complement(1887..>2919)
                     /locus_tag="VFP02_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET9541713.1"
                     /translation="FDVERGAAGMDTAFVAKAGTGDGPTIGVFCEYDALPEIGHACGH
                     NVIGAAGLGAGLAAATVAEELGGRVVVLGSPAEEGGGGKVFLEERGALDGLDAAMMIH
                     PADGDLQAMNVIAIQQVHVEYHGQAAHAAAFPHKGRNALDAAVLGYMNVAALRQHIRP
                     TERIHGVFTDAGDKPNIVPKYAAAEWYVRSPTLRLLQGLKERVLTCLEAGALASGCTM
                     DHEWQDPAYADMISNPVLVDLFSANAERTGRNLADPDLAAGVVGSTDMGNVSYVVPSI
                     HPMLAVAPPGISIHTPEFTAFARGEEGDRAVLDGARAMAMTVADLWLDPSRVEAAKAS
                     FDEAVATRR"
BASE COUNT          404 a          948 c         1101 g          466 t
ORIGIN      
        1 cgaggtcgtc ggcgacgacg aggtcttcgt ccaggtcctc ggcatccagg tcctcggcgt
       61 cgagatccac cgtctcggcg tcagcgtcct cgtcgatgtc caccgcgtcg tcttgtttag
      121 ccatgcctct cctcgtggga acgccctccg ggcgatcgcg ggcggatcat agaggcgcgt
      181 tcgcgcgttc gtgcgcccgg gtcagggggt gaggcgccgg gcctcggccc gacgctcggc
      241 atcgagtcgt tccaggtcgg gctcggggtc gtgatcgacg tagcgcatga gctcggccac
      301 ggcgtggtgg ccggcctcgt cggcgatgcg ctggtgcatc tgctgggcca cccaccggta
      361 ggcgggatgg ccctggggcg tggtccgcaa ctccagcatg tgcatcgcgg cccgggcgtt
      421 gatctgcatg acgaagcgga tccggtaggc cagggcgacg gcgtaggagg cctggtcggg
      481 aaaggccggc tcgaggtcgt cgaacagggc ctcggaccgc tccatggcct cgtcgaagcg
      541 ggcggcctgg ccggcggcat cgaccgactc gggccgggtg aagccgtgcc gcggggtcag
      601 gcgttgccac tcgatggtca gcatccggtg gcgctgcagg tcccggaacg cgccgtagtc
      661 ggtgaggacg tcgaaccggt agaaggagcg ctcgagggcc cgccccggct tgtggcgacg
      721 gttggaccgt tcgccgcagt aggcccgcac cacggccagc cgttcgtcgg tcgacatgga
      781 tcgtacccgg gcctcgatct cggtctcggg tcggtcggtg tgggcgtaga gcatgctcgc
      841 caccatcttc acctcggcgt ccgggtcgaa gtcgaccagg gtcaccgccg gctccgcgtc
      901 catctcgccc gaggtcggga agaccttggc cgccatctcg tccatggcca tgcggttctc
      961 cgccaggtag ttgctccacg ccaccccgcg gtcgggcacg tcgacccggc gcaggaagga
     1021 ggggatgacc ttgcgcagct cggtcagcat catccccgcg tacgagcgag cctcgggcag
     1081 agggtgggcc cgcatgcgca ggaggagttg ctcgtagccc tgaccggtgc cgtagatgcc
     1141 gacgttggag agggatgccg cgggcaggat cccccgcagg gcgtcgaagg ccttggcccg
     1201 gatcgactgg cggtagatga agtcggagtc ggcggggtcc ttggggatgt tgtcgcggaa
     1261 gaagtccccc agggtcggga cgagctcggc gtaggtgtcg aacagccggt ccatgtcgcc
     1321 gacgtagcgg gtgcccaggt tcgactggag aacgtcggga tcgcggtagt agcggaaccg
     1381 gccgccgatg cgggcgtcgt aggcgatgta gcgggtggac tgctcgaggt aggccatgag
     1441 ccggccccac tcgaggatct tggtcaggat gttcgaggcc tgctcgcagg ccaggtgcac
     1501 cccgccgagc tgggccaccg agtcgtcgcc gtagtcgtag aagacggtct ggtacagctc
     1561 ttcggcccgc tccaggccca tggtggcgtc gacggtctgg tcgccggtga tgtccaggtc
     1621 caccacgaac tcgtccagga acaggcggcg caggctcttg ggcgatcggg agtagcgggc
     1681 gaagagcgcg cccttgacca cctcgggcag gttgacgagg gcgaagaccg gacggtcgag
     1741 gttcgtgaag taccgccgga ggatgtcctc ctcatcggag ctgaattcct cggcgacgta
     1801 gacgctcatt cggggagtca gcgtacggcc tcaccgaccg aggacgggtg accgctcgcc
     1861 gtgctccccc gggtcacctg acgcggtcaa cgccgggtcg cgacggcctc gtcgaaggac
     1921 gccttggcgg cctcgacccg gctgggatcc agccacaggt cggccaccgt catggccatg
     1981 gcccgggcgc cgtcgagcac ggcccggtct ccctcctcgc cccgggcgaa ggccgtgaac
     2041 tcgggggtgt ggatcgagat gccgggcggg gcgacggcga gcatggggtg gatggagggg
     2101 acgacgtagc tcacgttgcc catgtccgtg ctccccacca caccggcggc gaggtcggga
     2161 tcggccaggt tccgcccggt gcgttcggcg ttggcgctga agaggtcgac gaggaccggg
     2221 ttggagatca tgtcggcgta ggccgggtcc tgccactcgt ggtccatggt gcagcccgac
     2281 gccagggccc ctgcttccag gcaggtcagg acccgctcct tgagcccctg gaggaggcgc
     2341 agcgtcggcg agcgcacata ccactcggcc gccgcgtact tggggacgat gttgggcttg
     2401 tccccggcgt cggtgaagac gccgtggatc cgctcggtgg gccggatgtg ctgacggagg
     2461 gcggcgacgt tcatgtagcc gagcacggcg gcgtcgaggg cgttgcgccc cttgtgcggg
     2521 aaggcggcgg cgtgggcggc ctggccgtgg tattcgacgt ggacctgctg gatggcgatg
     2581 acgttcatgg cctgcaggtc cccgtcggcg gggtggatca tcatcgccgc gtcgaggccg
     2641 tcgagggccc ctcgctcttc caggaagacc ttgcccccgc ccccctcctc ggccggggag
     2701 ccgaggacga cgacccggcc gccgagttcc tcggccacgg tggccgcggc caggccggcg
     2761 cccaagccgg cggcgccgat gacgttgtgg ccgcaggcat ggccgatctc cggcagcgcg
     2821 tcgtactcac agaagacccc gatggtcggg ccgtcgccgg tgccggcctt ggccacgaac
     2881 gcggtgtcca tgccggccgc gccccgctcg acgtcgaag
//
LOCUS       DASTFV010000080         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_275750, whole genome shotgun sequence.
ACCESSION   DASTFV010000080 DASTFV010000000
VERSION     DASTFV010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275750"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..376
                     /locus_tag="VFP02_01440"
     CDS             32..376
                     /locus_tag="VFP02_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HET9541714.1"
                     /translation="MHELSVGQAIVDIARERSDGCPVERVSVRIGYLRQVVPDALQFA
                     WEMLTEGTDLDGCVLDIEHVPAVVACASCSARTTLDWPVMGCGSCGGIDVELLSGEEF
                     QLASIDIGQEVG"
     gene            376..1158
                     /gene="hypB"
                     /locus_tag="VFP02_01445"
     CDS             376..1158
                     /gene="hypB"
                     /locus_tag="VFP02_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HET9541715.1"
                     /translation="MGRFHRHEDGTVHDHPHTEDHEHDRPVEARDVGDHRGYVDTGVS
                     RVAVLEKILSENDRVADGNRSAFAAAGVRAVNLMSGPGAGKTTLLRRTIVALGEQVRI
                     GVLEGDIATSLDADRLDGLGADISLVNTSAGFGGECHLDAVMVRAGLARLQLDRLDLL
                     VIENVGNLVCPAEFNVGESAKAMVYAVTEGEEKPLKYPVMFRAAEVVVVNKVDLLPHL
                     DFDLDRFLSNLGDVNPAAEVILTSARTGEGVDAWCAWLQGFA"
     gene            1159..1920
                     /locus_tag="VFP02_01450"
     CDS             1159..1920
                     /locus_tag="VFP02_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541716.1"
                     /translation="MLGIFTLGFRHGFDVDHVAAITDISGSQLFRLRAFVLSTLYALG
                     HALMVVALGLGIAWAGWDVPDAGRVVGLTLVVLGGYVLWCVATDRRPSSRAGLVVRAM
                     GWFRARLAPTTFVEHEHEHGHDGSHQHTHAELVEGPGSAAEGAGTTVVVGHRHRHRHD
                     DIRAPYGVAGAIGVGLVHGIGAETPTQVLALAAGTASLVPFLFGLFLANTVVAVVAAI
                     GLTERRLRGLNAIVGVFSVYVGILYLLAMTPPLVG"
     gene            1942..2673
                     /locus_tag="VFP02_01455"
     CDS             1942..2673
                     /locus_tag="VFP02_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET9541717.1"
                     /translation="MGDPPELLPFPEDWSRALAVVAHPDDLEYGTASAVAKWTDQGKW
                     VGYVLATSGEAGIDGLAPAEAGPLRESEEIASAAVVGVDSVEFLGLADGTLTYGLDLR
                     REIAAAIRRHRPELVLSINFHFSWGGPSVNMADHRVLGEALLDATRDAGNRWVFTDLL
                     DEGLEPWSGVRLAAFNGSPEATHVVDVTGFLDQGVASLEQHRAYLEGLGDGTTRAADV
                     VTPAAVATGARIGVEHAVAFEVFTL"
     gene            2707..>3710
                     /locus_tag="VFP02_01460"
     CDS             2707..>3710
                     /locus_tag="VFP02_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9541718.1"
                     /translation="MSDYTPPMDDIAFVLTHLCDIEDLSKLEPFTHADPATILGVIEE
                     AGRFMASAIAPLNHPADKQGLTFADGVITTPDGFKEAYAQYVDAGWGAVPFDPEYGGG
                     GFPWVVGVAMQELLTTASMAFSLGPLLTQGAIDMLQHHSSADQKAVYLPKMVTGEWTG
                     TMNLTEPQAGSDVGALITKALPAADGTYRISGQKIFITFGEHDLADNIVHLVLARTPD
                     APPGTKGISCFIVPKYLVEEDGSLGERNGVSCVSIEHKMGIHASPTCTLAYEDAVGFL
                     VGDENEGMRYMFTMMNNARLSVGLEGLALAERSHQQAVAFAKERLQGRAVGAEPGTQS
                     P"
BASE COUNT          535 a         1314 c         1275 g          586 t
ORIGIN      
        1 cgcgaagtgg ccgtgagcat ggggcgacaa ggtgcatgag ctctcggtcg gccaggcgat
       61 cgtcgacatc gcccgcgagc gcagcgacgg ctgcccggtc gagcgggtct cggtgcgcat
      121 cgggtacctc cgccaggtcg tgcccgacgc cctgcagttc gcctgggaga tgttgaccga
      181 gggcaccgac ctcgacggct gtgtgctgga catcgagcat gtccctgccg tcgtggcctg
      241 cgcctcctgc tccgcccgca ccaccctgga ctggcccgtg atgggctgcg gcagctgtgg
      301 gggcatcgac gtcgagctgt tgagcggtga agagttccag ttggcgtcca tcgacatcgg
      361 acaggaggtc ggctagtggg acggttccac cgccacgagg acggcaccgt ccacgatcat
      421 ccgcacaccg aagaccacga gcacgaccgc ccggtcgaag cccgcgacgt cggtgaccac
      481 cggggctacg tcgacaccgg tgtgtcccgg gtcgccgtgc tggagaagat cttgtcggag
      541 aacgaccgcg tcgccgacgg caaccggtcg gcgttcgccg ccgccggcgt tcgcgcggtc
      601 aacctcatgt ccggtcccgg cgcgggcaag accacgctgt tgaggcggac catcgtcgcc
      661 ttgggcgagc aggtgcgcat cggggtcctg gagggtgaca tcgccaccag tctcgatgcc
      721 gatcgcctgg acgggctcgg ggccgacatc tcgttggtca acaccagcgc cggcttcggt
      781 ggcgagtgcc acctcgacgc cgtcatggtg cgagccgggc tggcccggct gcagttggac
      841 cgcctcgacc tcctggtcat cgagaacgtc ggcaacctcg tctgcccggc ggagttcaac
      901 gtcggcgagt cggcgaaggc aatggtctat gcggtgacgg aaggcgagga gaagccactc
      961 aagtacccgg tcatgttccg agcggccgag gtggtggtgg tcaacaaggt cgacctcctc
     1021 ccgcacctcg acttcgatct cgatcgcttc ctcagcaacc tgggggacgt gaaccctgca
     1081 gccgaggtga tcctcaccag cgcccgcacc ggtgagggcg tcgacgcctg gtgcgcctgg
     1141 ctgcaaggat tcgcctgagt gctgggcatc ttcaccctcg ggttccgaca cggcttcgac
     1201 gtggatcacg tcgcggccat caccgacatc agcggcagcc agctcttccg gctccgggcg
     1261 ttcgtgctgt ccacgctcta cgccctcggg cacgcgctca tggtcgtggc cctcggcttg
     1321 ggaatcgcct gggcgggttg ggacgtgccc gacgcgggtc gcgtcgtcgg cctgacgctg
     1381 gtggtgctgg gcgggtacgt gctctggtgc gtggccaccg accggcgccc cagctcgcgc
     1441 gccggcctcg tggtccgagc catgggctgg ttccgggccc ggctggcacc gaccaccttc
     1501 gtcgagcacg aacacgagca cggccacgac ggcagtcacc agcacaccca tgccgagctg
     1561 gtcgagggtc ccggatccgc ggcggagggc gcaggcacca cggtcgtggt cggccatcgt
     1621 caccggcacc gccacgacga catccgggcc ccctacggcg tcgccggggc catcggcgtc
     1681 ggcctcgtgc acggcatcgg ggccgagacc ccgacccagg tgctggctct cgccgcgggc
     1741 acggcgtcgt tggtgccctt cctgttcggc ctgttcctgg ccaacaccgt cgtcgccgtc
     1801 gtcgccgcca tcgggctgac cgagcgtcgc ctgcgggggc tcaacgccat cgtcggcgtc
     1861 ttcagcgtct acgtcgggat cctctacctg ctggcgatga cgcccccctt ggtcggttga
     1921 ccgtgggggt acggtccgcg gatgggcgat ccgccggagc tgctgccgtt ccccgaggac
     1981 tggagccggg cccttgcggt ggtggcccat cccgacgacc tggagtacgg gacggcgtcg
     2041 gcggtcgcca agtggaccga tcagggcaag tgggtcgggt acgtcttggc caccagtggt
     2101 gaagcgggca tcgacggcct ggccccggcg gaggccggcc cgctgcggga atcggaggag
     2161 atcgcgtcgg ccgccgtcgt cggtgtggac tccgtcgagt tcctgggcct ggccgacggg
     2221 accctgacct acggcctcga cctgcgccgc gagatcgccg cggccatccg ccgccaccgg
     2281 cccgagctcg tgctgtcgat caacttccac ttctcgtggg gcggcccgtc ggtgaacatg
     2341 gccgaccacc gggtcttggg cgaggccctg ctcgacgcca cccgggacgc cggcaaccgg
     2401 tgggtcttca ccgatctcct ggacgagggg ctggagccgt ggtccggcgt ccggctggcg
     2461 gccttcaacg gctcgccgga ggccacccac gtcgtcgacg tgaccggttt cctggatcaa
     2521 ggtgtggcct cactcgaaca gcaccgggcc tacctcgaag gtctcggcga cggcacgacg
     2581 agggcggccg acgtggtcac ccccgcggcc gtggccacgg gggcccgcat cggtgtcgag
     2641 cacgcggtgg ccttcgaggt gttcaccctc tgaacccggc cgaccactgc tcggtaccct
     2701 cggggggtgt ccgactacac gccgccgatg gacgacatcg ccttcgtcct gacccacctc
     2761 tgtgacatcg aggacctgag caagctcgag ccgttcacgc acgcggaccc ggccacgatc
     2821 ctcggtgtga tcgaggaggc gggccggttc atggcctccg ccatcgcccc cttgaaccac
     2881 ccggccgaca agcagggcct gaccttcgcc gacggtgtca tcacgacgcc ggacgggttc
     2941 aaggaggcgt acgcccagta cgtcgatgcc ggttggggcg cggtgccctt cgatcccgag
     3001 tacggcggcg gcggcttccc ctgggtcgtc ggcgtggcca tgcaggagct cctgaccacg
     3061 gcctcaatgg ccttctcgct gggccccctt ctcacgcagg gcgccatcga catgctccag
     3121 caccacagtt cggccgacca gaaggccgtc tacctgccca agatggtgac cggtgagtgg
     3181 acgggcacga tgaacctgac cgagccccag gccggctccg acgtcggggc gctgatcacc
     3241 aaggccctcc ccgcggccga cggcacctat cggatcagcg gccagaagat cttcatcacc
     3301 ttcggcgagc acgacctggc cgacaacatc gtccacctcg tcctggcccg cacccctgac
     3361 gccccgccgg ggaccaaggg gatctcctgc ttcatcgtcc ccaagtacct ggtcgaggag
     3421 gacggttcgc tgggcgagcg caacggggtg agctgcgtgt ccatcgagca caagatgggc
     3481 atccacgcga gccccacgtg caccctcgcc tacgaggacg ccgtcggctt cctcgtcggc
     3541 gacgagaacg aaggcatgcg ctacatgttc acgatgatga acaacgcccg cctgtcggtg
     3601 gggctggagg gcctggccct cgccgagcgc tcccaccagc aggccgtggc cttcgccaag
     3661 gagcgcctcc agggtcgcgc cgtcggggcc gagcccggca cccagtcacc
//
LOCUS       DASTFV010000081         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_275763, whole genome shotgun sequence.
ACCESSION   DASTFV010000081 DASTFV010000000
VERSION     DASTFV010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275763"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1790
                     /locus_tag="VFP02_01465"
     CDS             <1..1790
                     /locus_tag="VFP02_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9541719.1"
                     /translation="GDDDFTYFWSGLRLAPGTDLADVEQAWEEQAPDRFAGRNPFRYL
                     RTSTLHASVQQANRPQVVGLAGFGLMAALLGTALTVQVTRRVVRAARPDLASLQALGG
                     TWARSSAGVLLAAGLAVLIGAALAVGLAWLLSPVAPVGLVREIEPDPGQAFDALVLGV
                     GGIVLALLGAVAAVLTARRVGRADEQAPADPGLAAHLPLVSGFGVRMVMGRRSTAARS
                     AVVGAVVSTVAVVATLVVGSNVQHLITTPRLFGADFDAAYELGSGYSQFDPEVLNRFL
                     TERDDPDLDGWSSVTYTNVTIGEVEVPALGVTPGRGTIGPTLLEGRAPAGPGEIALGE
                     DSLDAVGAAVGDEIDVEGQPFTVVGTAVLPALGQATSDHSGLGVGGWLAAEDLAGLFP
                     DEEFTGLASAVLVDFRDGADAEATVEGLKAELLDDLLAWGVSEADAEEAGADILTIYR
                     PPRPAELVGTASSLAAPALLAAMLGVGALIALGFALIASVRGRRRELAVVKSLGFLRR
                     QVQGVVLTQTLVTVVVGLVVGIPVGIALGGVLWRLLAEQLGVVAEQDIPTLAILAYAG
                     AGAVLAILLTAVPARIAARTPAALVLTQE"
     gene            complement(1803..>2255)
                     /locus_tag="VFP02_01470"
     CDS             complement(1803..>2255)
                     /locus_tag="VFP02_01470"
                     /inference="COORDINATES: protein motif:HMM:NF019829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HET9541720.1"
                     /translation="WEPDVDAEVERLGVDEYVTRAITDELVVGGESDPRALASRLWAL
                     EGVAKRYREFLIAYQDVPEALEAMRARHERLTDAEFLAGALATFVDFQEVVRHDPLLP
                     PELLPRPWPGREARILLAKVRKLGVLAREHHDRPVLFHQFDEVLDDLS"
BASE COUNT          276 a          788 c          857 g          334 t
ORIGIN      
        1 ggggtgacga cgacttcacc tacttctggt cggggctccg gctggcgccg ggcactgatc
       61 tcgccgacgt ggagcaggcg tgggaggaac aggcccccga ccggttcgcc ggccggaacc
      121 ccttccggta cctccgtacc agcacgctcc acgccagcgt ccagcaggcc aaccgtcccc
      181 aggtcgtggg cctggccggc ttcgggctga tggccgccct gctcggcacc gccttgaccg
      241 tgcaggtgac gcggcgggtg gtgcgggccg cccgacccga cctggcgtcc ctgcaggccc
      301 tgggcgggac ctgggccagg agctcagccg gcgtgctcct cgcggcgggc ctggcggtcc
      361 tgatcggcgc cgccctggcc gtcgggctgg cttggctgtt gtcgccggtg gcgcccgtgg
      421 gcctcgtgcg cgagatcgaa cccgacccag ggcaagcctt cgacgcgctg gtcctggggg
      481 tcggcgggat cgtgctcgcc ctcctcggcg ccgtggccgc cgtgctcacc gcccgacggg
      541 tggggcgggc cgacgaacag gccccagccg accccggtct ggccgcccac cttcccctgg
      601 tgagcgggtt cggcgtccgg atggtgatgg ggcgacggag cacggcggct cggtcggcgg
      661 tcgtcggggc cgtggtgtcg acggtcgcgg tcgtcgccac cctggtggtg ggcagcaacg
      721 tccagcacct gatcacgacg ccccgcttgt tcggcgccga cttcgacgcc gcctacgagt
      781 tgggttcggg gtactcccag ttcgaccctg aggtcttgaa ccggttcctg accgaacggg
      841 atgaccctga cctggacggc tggtcatcgg tcacctacac gaacgtgacg atcggcgagg
      901 tcgaggtccc cgccctcggc gtgactccgg gccgggggac catcgggccg accctcctcg
      961 agggccgagc gcccgccggg ccgggcgaga tcgccttggg cgaggacagc ctcgatgccg
     1021 tcggcgcggc cgtgggtgac gagatcgacg tcgagggcca accgttcacg gtcgtcggca
     1081 cggccgtgct ccccgcgctc gggcaggcca cgagtgatca cagcggcctc ggcgtcggcg
     1141 ggtggctggc ggccgaggac ctcgcgggcc tgttccccga cgaggagttc accggactgg
     1201 ccagcgcggt gctcgtcgac ttccgggacg gcgccgacgc ggaggcgacc gtcgaaggac
     1261 tgaaggcgga actgctcgac gacctgttgg cctggggggt gtccgaggcg gacgccgagg
     1321 aggcgggggc cgacatcctc acgatctacc gaccaccccg tccggccgag ctggtcggca
     1381 ccgcgagctc cctggccgca ccggcgttgc tggccgcgat gctcggcgtc ggcgccctca
     1441 tcgctctggg cttcgccctc atcgcctccg tacgggggcg tcgccgggag ctggccgtcg
     1501 tgaagtcgtt gggcttcctc cgccgccagg ttcagggggt cgtgctgacc cagaccctcg
     1561 tgacggtcgt cgtcggtctc gtcgtgggca tccccgtcgg gatcgccctg ggcggtgtcc
     1621 tgtggcgcct cctcgccgag caactgggcg tggtggcaga acaggacata ccgacgctgg
     1681 cgatcctggc ctacgcagga gccggagccg ttctggcaat cctgctcacc gccgtaccgg
     1741 cccggatcgc ggcgcggacc ccggccgcgc tggtcttgac ccaggagtag cgcccgggga
     1801 aatcagctga ggtcgtcgag gacctcgtcg aactggtgga acaggacggg gcggtcgtgg
     1861 tgctcgcggg cgaggacgcc gagcttgcgg accttggcca gcaggatgcg ggcctcccga
     1921 cccggccacg ggcgggggag cagctcgggc gggaggaggg ggtcgtgacg gacgacctcc
     1981 tggaagtcca cgaaggtggc gagggcgccg gccaggaact cggcgtcggt gaggcgctcg
     2041 tggcgggccc gcatcgcctc caaggcctcg ggcacgtcct ggtaggcgat gaggaactcg
     2101 cggtagcgct tggccacccc ctcgagggcc cacaggcggg aggcgagggc gcgggggtcg
     2161 gactcacccc ccaccaccag ttcgtcggtg atggctcggg tgacgtactc gtccacgccg
     2221 aggcgctcga cctcggcgtc gacgtcgggc tccca
//
LOCUS       DASTFV010000082         2999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_278106, whole genome shotgun sequence.
ACCESSION   DASTFV010000082 DASTFV010000000
VERSION     DASTFV010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278106"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..179
                     /locus_tag="VFP02_01475"
     CDS             15..179
                     /locus_tag="VFP02_01475"
                     /inference="COORDINATES: protein motif:HMM:NF026213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541721.1"
                     /translation="MAAQVAEWAVQAAPQDASAHGARAEVYAARRAEEPSLMAKGIFR
                     AAEGDSRPSG"
     gene            273..1205
                     /locus_tag="VFP02_01480"
     CDS             273..1205
                     /locus_tag="VFP02_01480"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HET9541722.1"
                     /translation="MSDAIAPVPISDETTRRLFGEPSFRVTKRGGYDPGDVAEFLGGL
                     GSQMLTLIGRLRKAESDVDVLTTELDHWQARTRDAEATRETFERTLALAEDTANATVA
                     DARHRAAGVMTNAEATADELIGQARHQSFAMVEQARDAAQRCYADERLKVREEWQRVQ
                     DESAQLETLRLAVAAETMALEEVRNQLRTRLRMAAAEMLNVAESPGCLGQPVTRGMPE
                     RPHPLAPSDPVQMPVASTVSAAPTDHVLEAAAAAVANPVVTPPPEPPEITPPAIEPIG
                     VGEGFGNPSADAAFERFMSEDIDDEPSREWILAG"
     gene            complement(1305..2141)
                     /locus_tag="VFP02_01485"
     CDS             complement(1305..2141)
                     /locus_tag="VFP02_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9541723.1"
                     /translation="MSLSRRQFLLAGAAGVVAGTAVGCGFFDDDGPERIAYGDAPSQF
                     GDLYLPSGDPQATVVLIHGGGWETSEDLSLVRPMARDLRREGYAVWNVEYRRVGEAGG
                     GWPGTFEDVGAAIDHVNALADDHPVDPEMVVVVGHSAGGTLALWAAGRDGDVEPRGYL
                     SLAGFTDLEACVQENLLAGACARLLGGTPAEVSRRYQDASPLERLPTGRRQSLVHGED
                     DDIVPVRQSEGYAAAAEAAGDPVTLVTVPNAGHFQLIDIHHPAYGQVLDQIRTLVSLN
                     PA"
     gene            complement(2151..>2999)
                     /locus_tag="VFP02_01490"
     CDS             complement(2151..>2999)
                     /locus_tag="VFP02_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9541724.1"
                     /translation="GAGQMIDRARAAYAAGLEGLFVGDHHATSVPYYQNSPILGRLMA
                     EWPDRRFGALYLLPLWHPLVVAEQIGTLASLGPGPFVFQCAIGGGEDQFGPMGIPLDG
                     RGGRFEASLAAIRALLAGEVVDDPAGPWGLRRARISPTPPEPVEIWIGGAGPKALARA
                     ARLGDTWYAGPELTDDDAEQKLAQYLDHGADPPCRPLRRDIYVGADEADVARVREPLE
                     QKGYRGFDLAALVTGTAEQVAERFLSLGERGFDEIVVRQLSFDQTDALASLERLAEVR
                     RLVAAG"
BASE COUNT          437 a         1120 c         1068 g          374 t
ORIGIN      
        1 cgggtgacct ccgcctggcc gcccaggtcg cggagtgggc cgtgcaggcc gcaccgcagg
       61 atgccagcgc ccacggggct cgggccgagg tctacgccgc ccgacgggcc gaggagcctt
      121 cgctcatggc caagggcatc ttccgcgccg ccgaagggga cagccgtccc tcaggctgac
      181 ccgggagggg cccggaccct tctgctcaag tccccaaagc agatgccgac ccttgaccta
      241 ggtcattcgg cctgatccag gagacacgca ccatgtccga cgccattgca cccgttccga
      301 tcagcgacga aaccactcgt cggctgttcg gcgagcccag ttttcgggtc accaagcgag
      361 gcgggtacga ccccggtgat gtggccgagt tcctgggcgg cctgggcagc cagatgctca
      421 cgctcatcgg tcggctgcgc aaggccgagt ccgatgtcga cgtcctgacg accgagctgg
      481 accactggca ggcccgcacc cgcgacgccg aagcgacccg cgagaccttc gagcgcaccc
      541 tcgccctggc cgaggacacg gccaacgcca ccgtggccga cgcccgccac cgggccgccg
      601 gcgtcatgac caacgccgag gccaccgccg acgagctgat cggacaggcc cgccaccagt
      661 ccttcgccat ggtcgagcag gccagggacg cggcccagcg gtgctacgcc gacgagcgcc
      721 tgaaggtgcg cgaggagtgg cagcgggtgc aggacgagtc cgcgcagctg gagaccctcc
      781 gtctcgccgt ggccgccgag accatggccc tcgaagaggt tcgcaaccag cttcggaccc
      841 gccttcgcat ggccgccgcc gagatgctga acgtggccga gagccccggc tgcctgggtc
      901 agcccgtgac ccggggcatg ccggagaggc cgcaccctct ggccccgtcg gaccccgtcc
      961 agatgcctgt ggcgtccact gtcagcgccg cgcccaccga ccatgtcctg gaagccgccg
     1021 ccgcggccgt ggccaacccg gtcgtgacgc cgccgccgga gccacccgag atcaccccgc
     1081 cggccatcga gcccatcggg gtgggcgagg gcttcggcaa cccgtcggct gacgccgcct
     1141 tcgagcgctt catgtccgag gacatcgacg acgagcccag ccgcgagtgg atcctcgccg
     1201 gctagaccgt cacatcgctc cgccttcgct ccgccggctc cgccgaagcg gcgggtcgaa
     1261 ggcgcggcac cgccgcctcc gcagcgtcac gccgcggccg cccgtcaggc ggggttcaac
     1321 gagaccaggg tcctgatctg gtccagcacc tggccgtagg cggggtggtg gatgtcgatg
     1381 agctggaaat ggccggcgtt gggcacggtc accagggtca ccgggtcgcc cgcggcctcc
     1441 gccgccgcgg cgtagccctc ggactgacgg accgggacga tgtcgtcgtc ctccccgtgg
     1501 acgaggctct ggcgtcggcc cgtgggcagg cgctccagcg gtgaggcgtc ctggtagcgc
     1561 cggctgacct ccgccggtgt cccgccgagc agtcgggcac aggccccggc caacaggttc
     1621 tcctgcacgc aggcctcgag atcggtgaac ccggccagcg acaggtaacc ccgaggctcg
     1681 acgtcgccgt cccgacctgc ggcccagagg gccagcgtcc cgccggcgga gtggcccacg
     1741 accacgacca tctccggatc gacagggtgg tcgtcggcca acgcgttcac gtggtcgatg
     1801 gcggcaccga cgtcctcgaa cgtaccgggc cacccgccgc cggcttcgcc gacgcggcgg
     1861 tactcgacgt tccacacggc gtacccctcg cgtcgcaggt cccgggccat ggggcggacg
     1921 agcgacaggt cttcgctcgt ctcccaaccc ccgccgtgga tcagcaccac cgtggcctgc
     1981 gggtccccgc tgggcaggta gagatcgccg aactgggaag gggcgtcgcc gtaggcgatg
     2041 cgctcgggcc cgtcgtcgtc gaagaagcca cagccgacgg cggtgccggc gacgacgccg
     2101 gcggcgccgg cgagcaggaa ctggcgacgg gagaggctca cccggtggtg ctaccccgcg
     2161 gcgacgagcc gacgaacctc ggccagtcgc tcgagagacg ccagggcgtc tgtctggtcg
     2221 aaggagagct ggcggacgac gatctcgtcg aagccccgct cgccgaggct gaggaagcgc
     2281 tccgccacct gctcggcggt gccggtgacc agggcggcga gatcgaagcc ccggtagccc
     2341 ttctgctcca agggctcccg cacccgggcc acgtccgcct cgtcggcgcc gacgtagatg
     2401 tcccggcgca gcggtcggca cggcgggtcg gcgccgtggt ccaggtactg ggccagcttc
     2461 tgctcggcgt cgtcgtcggt cagctcgggc ccggcgtacc aggtgtcacc gagccgcgcg
     2521 gcccgggcca gggccttggg cccggccccg ccgatccaga tctccacggg ctccgggggc
     2581 gtcgggctga tccgggctcg gcgcaagccc cacgggccgg cggggtcgtc gaccacctcg
     2641 cccgccagga gggcccgaat ggcggccagg gatgcctcga agcgcccgcc ccgcccgtcg
     2701 agtgggatgc ccatcggccc gaactggtcc tcgcccccac cgatggcgca ttggaacacg
     2761 aacgggccag ggccgagcga ggccagcgtc ccgatctgct cggcgaccac cagggggtgc
     2821 cagaggggca gcaggtagag ggcgccgaag cgccggtcgg gccactcggc catgaggcgg
     2881 cccaggatcg gcgagttctg gtagtaggga acggacgtgg cgtggtggtc gcccacgaac
     2941 agcccctcga ggccggcggc gtacgccgcc cgggcccggt cgatcatctg cccggcgcc
//
LOCUS       DASTFV010000083         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_290026, whole genome shotgun sequence.
ACCESSION   DASTFV010000083 DASTFV010000000
VERSION     DASTFV010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290026"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VFP02_01495"
     CDS             <1..1204
                     /locus_tag="VFP02_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET9541725.1"
                     /translation="REVGVTPEEIRRRNYIPSDAFPYDSPAGLVFDSGNYEPTLDRAL
                     ELAGLDDLRGEQQRLRDEGSTTHLGIGLCTYVEMCGLAPSRVLASLNYGAGGWESATV
                     RMLPTGSVQVVTGTSPHGQGHETTWSQIVADQLGIDPDAIEVLHSDTAIAALGMDSYG
                     SRSLAVGGTAVHMATDLVLEKARAIAAHQLEASADDLEFEDGSFRVRGSPDKSVAIGD
                     VAMAAFTAHDLPDGMEPNLSAQVTYDPPNFVFPFGTHVAVVEIDEETGDIRLRDYVAV
                     DDCGNQINPMIVDGQLHGGILQGVAQALWEGAAYDEDGNLNNASLADYLVPSAMEAPT
                     FTLDSTVTPSPTNPLGVKGIGEAGTIGSAAAVMNAVIDALGPLGITDIDMPASPNKVW
                     AAIQEARS"
     gene            1201..2022
                     /locus_tag="VFP02_01500"
     CDS             1201..2022
                     /locus_tag="VFP02_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET9541726.1"
                     /translation="MIPVAFDYVRAGSADEALALLAEHGDEAKLLAGGHSLLPLMKLR
                     LAVPGVLVDIGRLDDLSYIREDGDTIAIGALTRHRDIETSDVLKEHVPLLAHATSKVG
                     DPQVRHRGTLGGALSHGDPAADHPAVLLALGGEMVIRGPGGERTVAADDFFTGFLETA
                     LAPDELLTEVRIPKATGAGWSFQKFNRRAQDWAIVGVAAVHNGRTGIGLVNMDTKPIR
                     ATASEEAMAGGASAADAADVAHEGTNPPADLNGTVEYRQHLARVLVRRALEEATA"
     gene            complement(2024..>2794)
                     /locus_tag="VFP02_01505"
     CDS             complement(2024..>2794)
                     /locus_tag="VFP02_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9541727.1"
                     /translation="RISTFAVARTLGGRSLVRIRRIPAAIIPTVAMPVFLLISFSGAF
                     SSVVNIPGFPTDSILSWVGPYAVIQGAAFAGVGASQSVAADLENGFYDRLLMSPSSRF
                     SLMLGPLSGAMVRVLLPFTVVLAVAFIGGLELTEGVRGLLMLLLTAEGVALIATFYGL
                     ALVYKFRTQRSGSLVQVGVFVVMFLSVGQVPLAVMEGWLHGVATVNPMTNILRMARQG
                     FVAQITWADTWPGLLVILLGTLFFGRWAYRGLLRLTQE"
BASE COUNT          464 a         1038 c          941 g          351 t
ORIGIN      
        1 ccgcgaggtc ggggtcaccc ccgaggagat ccggcggcgc aactacatcc ccagcgacgc
       61 cttcccgtac gactcccccg ccgggctggt cttcgacagc ggcaactacg agccgacgct
      121 ggaccgggcc ctggagctcg ccgggctcga cgacctgcgg ggcgagcaac agcgcctgcg
      181 ggacgagggc tcgaccaccc acttgggcat cggcctctgc acctacgtgg agatgtgcgg
      241 cctggccccc agccgggtcc tggcctcgct caactacggc gccggcggct gggagtccgc
      301 cacggtgcgg atgctgccga ccggcagcgt ccaggtggtc accgggacct cgccgcacgg
      361 tcagggccat gagaccacgt ggtcccagat cgtggccgac cagctcggca tcgaccccga
      421 cgccatcgag gtcctgcact ccgacacggc catcgccgcc ctcgggatgg acagctacgg
      481 ttcccgctcg ctcgccgtcg gcggcacggc cgtgcacatg gccaccgacc tggtgctgga
      541 gaaggcccgg gccatcgccg cccaccagct ggaggcatcg gccgacgacc tcgagttcga
      601 ggacggctcg ttccgggtgc ggggctcgcc ggacaagtcc gtggccatcg gcgacgtggc
      661 catggccgcg ttcaccgccc acgacctgcc cgacggcatg gagccgaacc tctcggccca
      721 ggtcacctac gacccgccca acttcgtctt cccgttcggc acccatgtgg ccgtcgtcga
      781 gatcgacgag gagaccggcg acatccgtct ccgggactac gtcgcggtcg acgactgcgg
      841 caaccagatc aacccgatga tcgtcgacgg tcagctccac ggcgggatcc tgcagggggt
      901 ggcccaagcc ctctgggagg gggccgccta cgacgaggac ggcaacctga acaacgcctc
      961 gttggccgac tacctcgtgc cctcggccat ggaggcaccg accttcacgc tggactcgac
     1021 ggtcacgccc agcccgacca acccgctcgg ggtcaagggc atcggggagg ccgggaccat
     1081 cggttccgcc gccgccgtca tgaacgcggt gatcgatgcc ctcgggcccc tcggcatcac
     1141 ggacatcgac atgcccgcct cgcccaacaa ggtgtgggcg gcgatccagg aggcccgctc
     1201 gtgatccctg ttgcgttcga ctacgtccgg gccggctcgg ccgacgaggc gctggccctc
     1261 ctcgccgaac acggcgacga ggccaagctc ctggccggtg gccactccct gctgccgctg
     1321 atgaagctgc ggctggccgt gcccggcgtg ctggtcgaca tcggtcgcct cgacgacctg
     1381 tcctacatcc gtgaggacgg cgacaccatc gccatcgggg ccctgacccg gcaccgtgac
     1441 atcgagacca gcgacgtcct gaaggagcac gtgcccctcc tcgcccacgc cacctcgaag
     1501 gtcggtgacc ctcaggtccg ccatcgcggc acgcttggcg gggcgctgtc ccacggcgat
     1561 ccggcggcgg accaccccgc cgtcctgctc gccctcgggg gcgagatggt catccggggc
     1621 ccgggcggcg agcgcacggt ggcggccgac gacttcttca ccggctttct ggagacggct
     1681 ctcgcccccg acgagctgtt gaccgaggtc cgcatcccca aggcgaccgg tgccggctgg
     1741 tcgttccaga agttcaaccg ccgggcccag gactgggcca tcgtcggcgt ggccgccgtg
     1801 cacaacggcc gcacgggcat cggcttggtc aacatggaca ccaagcccat ccgagccacg
     1861 gcgtccgaag aggccatggc cggcggggcc tcggccgccg acgcggccga cgtcgcccac
     1921 gaggggacca acccgccggc cgacctcaac ggcaccgtcg agtaccgcca acacctggcc
     1981 cgggtcctcg tccgccgcgc cctcgaagaa gcgaccgcct agctcactcc tgggtcaggc
     2041 gcaggaggcc gcggtaggcc cagcggccga agaacagggt cccgagcagg atcaccagca
     2101 ggccgggcca ggtgtcggcc caggtgatct gggcgacgaa gccctgccgg gccatgcgca
     2161 ggatgttggt catcgggttg accgtggcca caccgtggag ccagccctcc atcaccgcca
     2221 gaggcacctg cccgaccgag aggaacatca ccacgaagac gccgacctgc acgagcgagc
     2281 cggaccgctg ggtgcggaac ttgtagacca gggccagccc gtagaaggtg gcgatgaggg
     2341 ccacgccctc cgccgtcagc agcagcatca gcaggccccg gaccccctcg gtcagctcca
     2401 gaccaccgat gaaggccacc gccagcacca cggtgaaggg cagcagcacc cggaccatcg
     2461 cccccgacag gggcccgagc atcaggctga accgcgagct cggtgacatc agaagacggt
     2521 cgtagaaccc gttctcgagg tcggcggcga ccgactgcga cgccccgaca ccggcgaagg
     2581 ccgccccctg gatgacggcg taaggcccca cccaggagag gatcgagtcg gtcgggaagc
     2641 cggggatgtt gaccactgac gagaaggcgc cgctgaagga gatcagcagg aagaccggca
     2701 tggcgaccgt cgggatgatg gcggccggga tccggcggat ccgcaccagg ctgcggccgc
     2761 cgagcgtccg ggccacggcg aacgtgctga tccg
//
LOCUS       DASTFV010000084         5937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_291124, whole genome shotgun sequence.
ACCESSION   DASTFV010000084 DASTFV010000000
VERSION     DASTFV010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291124"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VFP02_01510"
     CDS             complement(<1..791)
                     /locus_tag="VFP02_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HET9541728.1"
                     /translation="MKVLLSWMREFAPELTDDVDVLSEALDDLGTPVEERVRLGEGLD
                     GIVVARVLETKPHPDADRVQLVDVDAGDGEALQIVCGAFNMAAGDLIPLATLGTTMPN
                     GMVIERRKMRGEWSNGMLCSATEIGLGEDSEGILILSPGLTPGQPLTEALGLQGDWLL
                     DLEVNANRPDALSMVGVARDVAARLGVGFTLPSWTAPTGGASSRAVASVEIVDAAACP
                     RFVARVLRDVTIGPSPTWLANRITQAGMRSISNVVDVSNYVMLELG"
     gene            complement(791..1789)
                     /gene="pheS"
                     /locus_tag="VFP02_01515"
     CDS             complement(791..1789)
                     /gene="pheS"
                     /locus_tag="VFP02_01515"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET9541729.1"
                     /translation="MIDEIARIQAGALARVQTASSVDELTTIGQEILGKRSELVGLRK
                     QLGGLDEATRKQTGEALNAATQVIKAALADRESGLQGAERSARYEAERLDLTEIIDPP
                     PVGHLHIVTQTWQALEDIFVGMGYRVAEGPEIETDWYNFEALNIPDSHPARSMWDTLW
                     VDIGEPGSMLLRTHTSNVQIHVMQAEAPPIYIVAPGRTYRRDTTDATHLPVFHQIEGL
                     VVDRGITMADLAGTIQAFTQAYFGQDFESRLRPSYFPFTEPSAEFDIRPPGGDWLELG
                     GCGMVHPNVFRHVGIDPEEWTGFAFGFGIDRLAKVRYEVDDLREFVTSDIRFLEQF"
     gene            complement(1836..2603)
                     /locus_tag="VFP02_01520"
     CDS             complement(1836..2603)
                     /locus_tag="VFP02_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET9541730.1"
                     /translation="MSKGSVQRVRRLGRHARARREEGAFVVEGPRAISAALDFDADLD
                     EVLVVGGAEVELVNRARDAGATITHVEGEQLERALTTRTPQPIVAVAGLVSWSPAEIV
                     SGATESGLPVVVLAGVSDPGNAGTVIRSAAAAGAAGVLFTPGSVDPTNPKCVRSSAGA
                     LFALRVGAVDDLDVLRDLGLTTIGAAAGGASPDEVDLSGPIALVLGGEAGGLPEDQLV
                     DVEVALPLEGGVESLNVAMAGSVLLYEARRQRNGGRW"
     gene            complement(2596..2964)
                     /gene="rplT"
                     /locus_tag="VFP02_01525"
     CDS             complement(2596..2964)
                     /gene="rplT"
                     /locus_tag="VFP02_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET9541731.1"
                     /translation="MARTKTSVPSRRRRKVTLERAQGYYGNKSRSYRAANEQVMHSLR
                     YAYRDRRARKGEFRRLWIQRINAACRENGTSYSRFIAGLKAAEVEVDRKILADLAVSD
                     PAAFTALVAVANEHAGSSDQ"
     gene            complement(2976..3173)
                     /gene="rpmI"
                     /locus_tag="VFP02_01530"
     CDS             complement(2976..3173)
                     /gene="rpmI"
                     /locus_tag="VFP02_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET9541732.1"
                     /translation="MPKMKTHRGAKKRFKVTGSGKVTRRQIHKNHFKRRKPADRQRRL
                     AGEVEVTGGDRQHVLRQLGKR"
     gene            complement(3209..3922)
                     /gene="infC"
                     /locus_tag="VFP02_01535"
     CDS             complement(3209..3922)
                     /gene="infC"
                     /locus_tag="VFP02_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET9541733.1"
                     /translation="MASGPAHDDTRINDQIRVREVRLVGVDGEQIGIKATPEALALAR
                     DQGLDLVEVAAAANPPVCRIMDYGKFKYENAQKAKESRKKATNVVVKEMKYRPKIGRG
                     DFDTKTRKVHEFLGEGHKVKVTIMFRGREVQHPELGRRILDRVAETVVDVGRVEALPK
                     VDGRNMTMVLGPDKQAQAKKAREDAEAAEALQAEANGSAEVVEAAPAPVVDSTPETNS
                     ATENAAENETSEAAATAAE"
     gene            4143..4577
                     /locus_tag="VFP02_01540"
     CDS             4143..4577
                     /locus_tag="VFP02_01540"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HET9541734.1"
                     /translation="MSSLWTPGGEHPVEPDAAPSDPTGPQTDPETDPLAGLSDEERAQ
                     AEAMAAEMAAARQRVLEAPADMVVANHAMGLYELAAIHLSEEDPDLTAARLAIDAMGQ
                     LVDGLSGRLGEAEATLKDALAQLRMAFVQVSEQAAGGDAPEG"
     gene            4817..5008
                     /locus_tag="VFP02_01545"
     CDS             4817..5008
                     /locus_tag="VFP02_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541735.1"
                     /translation="MDVDIKFWFYLAAVICFLLAAIGENWRYGARTRRGVAPALTLLP
                     LGLALFAFPTMWDVGVRAF"
     gene            complement(5005..5223)
                     /locus_tag="VFP02_01550"
     CDS             complement(5005..5223)
                     /locus_tag="VFP02_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HET9541736.1"
                     /translation="MADDLTGTVTTFDDPAGFGTVTADEGGEHFFHCTAIADGTRQIA
                     VGARVRFVVVPGRLGRWEAATVRPIDAA"
     gene            complement(5226..>5937)
                     /gene="hisG"
                     /locus_tag="VFP02_01555"
     CDS             complement(5226..>5937)
                     /gene="hisG"
                     /locus_tag="VFP02_01555"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HET9541737.1"
                     /translation="RYVAEGMFDLGITGRDWIEETSSEVVTLGELHYSKATARPIKMV
                     VAVGGDSDVQSVDDLPQGVRVSTEYPEVTRRYFEKQGIDADIRLSYGATEAKIPDIVD
                     CIVEITETGRALRAAGLRVIDEILQSYTELIANPAAYEDADKRHAMGQILTLLEGTLE
                     ARGKVLVKLNVAEADLQAVIDVLPAMKAPTVNALHGDGGFAVETVVPKREINTLIPEL
                     KDRGATDIIELPLAKIVH"
BASE COUNT          832 a         2057 c         2082 g          966 t
ORIGIN      
        1 cccagctcca gcatcacgta gttcgagacg tcgaccacgt tgctgatgga gcgcatgccg
       61 gcctgggtga tgcggttggc cagccaggtg ggcgacgggc cgatggtcac gtcccgcagc
      121 acgcgggcga cgaagcgggg gcaggcggcg gcgtccacga tctcgaccga agcgacagcc
      181 cggctggagg cgccacctgt cggcgcggtc caggagggca gggtgaagcc cacaccgagc
      241 cgagcggcca cgtcccgggc cacgccgacc atcgacagcg catcgggccg gttggcgttg
      301 acctcgaggt cgagcaacca gtcaccctgc aggcccagcg cctcggtcag gggttggccg
      361 ggggtcaggc ccggcgagag gatcaggatg ccctcgctgt cctcgcccag accgatctcc
      421 gtggccgagc agagcatccc gttcgaccac tcgccccgca tcttgcgccg ctcgatgacc
      481 atgccgttgg gcatggtcgt gcccaacgtg gccaacggga tcaggtcacc agccgccatg
      541 ttgaacgcgc cgcagacgat ctgcagcgcc tcgccgtcac ccgcgtccac gtcgaccagc
      601 tggacccggt cggcgtcagg atggggcttg gtctccagca cccgggccac gacgatgccg
      661 tccagcccct cgccgagccg cacccgctcc tcgaccggcg tgccgaggtc gtccaacgcc
      721 tccgacagca cgtcgacgtc gtcggtcaac tccggcgcga actcccgcat ccacgacaag
      781 agcaccttca tcagaactgc tcgaggaatc ggatgtcgga cgtgacgaac tcgcggaggt
      841 cgtcgacctc gtagcggacc ttggccaggc ggtcgatgcc gaagccgaag gcgaagccgg
      901 tccattcctc ggggtcgatg cccacgtggc ggaagacgtt gggatggacc atgccgcagc
      961 ccccgagctc cagccagtcc ccgcccgggg ggcggatgtc gaactcggcc gacggctcgg
     1021 tgaacgggaa gtaggagggg cgcaggcggg actcgaagtc ctggccgaag taggcctggg
     1081 tgaaggcctg gatggtgccc gccaggtccg ccatcgtgat cccccggtcg accaccaggc
     1141 cctcgatctg atggaagacc ggcaggtggg tggcgtcggt cgtgtcccgt cggtaggtcc
     1201 ggcccggggc cacgatgtag atgggcggtg cctcggcctg catcacgtgg atctgcacgt
     1261 tggaggtgtg cgtccgcagg agcatcgagc cgggctcgcc gatgtcgacc cagagggtgt
     1321 cccacatcga ccgggccggg tgcgagtccg ggatgttgag ggcctcgaag ttgtaccagt
     1381 cggtctcgat ctccggaccc tcggcgacgc ggtagcccat gcccacgaag atgtcctcga
     1441 gggcctgcca ggtctgggtg acgatgtgga ggtgcccgac cggcgggggg tcgatgatct
     1501 cggtgaggtc gaggcgttcg gcctcgtagc gggccgaccg ctcggcgcct tgcaggccgc
     1561 tctcccggtc ggccagtgcg gccttgatca cctgggtggc ggcgttcagg gcctcccccg
     1621 tctgcttccg ggtggcctcg tccagtccgc ccagctgctt gcggaggccg accagctcgg
     1681 agcgcttgcc caggatctcc tggccgatcg tggtgagctc gtcgacgctg gaggccgtct
     1741 ggacgcgggc cagggcgccg gcctggatgc gagcgatctc gtcgatcatg gggccaccac
     1801 cctccccgtt cggcaggacc tcggtccagt tggattcacc aacgaccccc gttgcgctgc
     1861 cggcgggctt cgtagagcag gaccgagccc gccatggcca cgttcagcga ctccacgccg
     1921 ccctccagcg ggagcgccac ctcgacgtcg accagctggt cctcgggcag accgccggcc
     1981 tccccgccga gcacgagggc gatcgggccg gacaggtcga cctcgtcggg gctggcaccg
     2041 ccggcggccg cgccgatcgt ggtcaggccc aggtcccgga ggacgtcgag gtcatcgacc
     2101 gctcccaccc ggagggcgaa gagggccccg gcactcgaac gcacgcactt ggggttggtg
     2161 gggtcgaccg accccggcgt gaagagcacg ccggccgcac cggccgccgc ggccgagcgg
     2221 atgacggtgc ccgcgttgcc ggggtcgctg accccggcca gtaccaccac gggcaggccg
     2281 ctctcggtgg ccccgctgac gatctccgcc ggtgaccagc tgaccagtcc ggccacggcc
     2341 acgatcggct ggggcgtgcg ggtggtcaac gcccgctcga gctgttcgcc ctcgacgtgg
     2401 gtgatggtcg ccccggcgtc ccgggcccgg ttgaccagct ccacctcggc cccgccgacg
     2461 acgaggacct cgtcgaggtc cgcgtcgaag tccagggccg cgctgatggc gcgtgggcct
     2521 tcgacgacga aggcgccctc ctcccgacgg gcgcgggcgt gtcgccccag gcgacgtacc
     2581 cgctggacgg agcccttact gatccgagga gcccgcgtgc tcgttggcga ccgcgaccag
     2641 ggcggtgaag gccgccgggt cgctgacggc caggtcggcc aggatcttgc ggtcgacctc
     2701 gacctcggct gccttcaggc cggcgatgaa ccggctgtag gaggtgccgt tctcccggca
     2761 ggcggcgttg atgcgctgga tccacagccg gcggaactcg cccttgcggg cccgacggtc
     2821 gcggtaggca tagcgcaggg agtgcatcac ctgctcgttg gcggcccggt acgaacggct
     2881 cttgttgccg tagtagccct gggcgcgctc gagggtgacc ttgcggcgac gacgcgacgg
     2941 gaccgatgtc ttcgttctgg ccatgactgc ctcctctacc gcttgcccag ctggcggagg
     3001 acgtgctggc gatcgccgcc cgtcacttcg acctcaccgg ccagacgacg ctggcgatcg
     3061 gcgggcttgc gccgcttgaa gtggttcttg tggatctggc ggcgcgtcac cttgccgctg
     3121 cccgtcacct tgaagcgctt cttggcgccc cggtgcgtct tcatcttggg catttgcgtc
     3181 tcttcctctc ggcggcggtg ccgctccctc actcggccgc ggtggccgct gcctcactcg
     3241 tctcgttctc ggctgcgttc tcggttgcgc tgttggtctc gggggtgctg tcgaccacgg
     3301 gggccggggc ggcctccacg acctcggcgg agccgttggc ctcggcctgg agggcttcgg
     3361 cggcttcggc gtcctcgcgg gccttcttgg cctgtgcctg cttgtccgga cccaggacca
     3421 tggtcatgtt gcgaccgtcg accttcggca gggcctcgac ccgaccgacg tccacgaccg
     3481 tctccgccac cctgtccagg atgcggcgcc ccaactcggg gtgctggacc tctcggcccc
     3541 ggaacatgat ggtgaccttg accttgtggc cttcgccgag gaactcgtgg accttgcgtg
     3601 tcttggtgtc gaagtcgccg cgcccgatct tggggcggta cttcatctcc ttgacgacga
     3661 cgttggtcgc cttcttccga gactccttgg ccttctgggc gttctcgtac ttgaacttcc
     3721 cgtagtccat gatgcgacag acgggggggt tcgccgcggc cgcgacctcg acgaggtcga
     3781 gaccttgatc gcgggccagt gcgagagctt ccggggtggc cttgatgccg atctgctcgc
     3841 catcaacacc taccaggcgg acctcacgga cccggatctg gtcattgatc cgggtgtcgt
     3901 catgggcggg gcccgaagct atggccgatc actacgcaat gcgcgactcc tcttgcgaac
     3961 gaaagagtcc cgatccccgg gccgcctcag acggtgagcc acaggagcga caaggaccac
     4021 ccgcaggggt ggtgctggcc aggacctgtg cgcaccgcag tcggtggcca ggtgggggcg
     4081 ggaccccgct ttccaggttg gattgcgagc aacaaggtac cagtggtggc acccttcccc
     4141 tcatgagcag cctttggacc cccggcggcg agcacccggt cgagccggat gccgcgccct
     4201 cagaccccac cggcccgcag acggaccccg agaccgatcc cctggccggc ctcagcgacg
     4261 aggagcgggc ccaggccgag gccatggccg ccgagatggc cgccgcccgc cagcgggtgc
     4321 tggaagcccc ggccgacatg gtcgtggcca accacgccat gggcctctac gaactggcgg
     4381 cgatccacct cagcgaagag gatcccgacc tgacggccgc ccgcctcgcc atcgacgcga
     4441 tgggccagct ggtcgacggg ctgtccggcc gcctgggtga ggccgaggcc acgctcaagg
     4501 acgcgctggc ccagctgcgc atggccttcg tccaggtcag cgagcaagcc gccggaggcg
     4561 acgcacccga gggctgacca tcagggtcac gggacagcgg gcgacggatc acccacgagt
     4621 tcgagagcgc aggtcttaat tcggcgcggc cgggtacgga ggtggcgcac gtcacccgga
     4681 tgtgcctaag gctcgagagg gggaccccat ggcgatcggc gttggcacct tgttgctcat
     4741 catcatcctg atcctgctgc tgacctgacc ggaccgatcg tccctcccat cagcccccga
     4801 gtcgaaaggc aatcccatgg acgtggacat caagttctgg ttctacctgg ccgccgtcat
     4861 ctgcttcctc ctcgccgcaa tcggcgagaa ctggcggtat ggcgcccgta cgcgccgagg
     4921 tgtggccccg gcgttgaccc tgctgccgct gggtctcgcc ctcttcgcgt tccccaccat
     4981 gtgggacgtg ggggtgcggg ccttctaggc ggcgtcgatc ggccgcaccg tcgcggcctc
     5041 ccagcgcccg aggcgcccgg ggacgaccac gaatcggacc cgggcgccga cggcgatctg
     5101 gcgggtgccg tcggcgatgg ccgtgcagtg gaagaagtgc tccccgccct cgtcggcggt
     5161 caccgtgccg aaccccgcgg ggtcgtcgaa cgtggtgacc gtccccgtga ggtcgtcggc
     5221 cacggttagt ggacgatctt ggccaggggg agctcgatga tgtcggtggc gccccggtcc
     5281 ttcagctcgg ggatcagggt gttgatctcc cgcttgggga cgacggtctc caccgcgaac
     5341 cccccgtcgc cgtgcagcgc gttgacggtg ggtgccttca tcgcgggcag gacgtcgatc
     5401 accgcctgga gatcggcctc ggccacgttc agcttcacca ggaccttgcc ccgggcctcg
     5461 agcgtgccct cgaggagggt caggatctgg cccatggcgt gacgcttgtc ggcgtcctcg
     5521 taggccgccg ggttggcgat gagctccgtg taggactgca ggatctcgtc gatgacccgc
     5581 aggccggcgg cgcggagagc ccgaccggtc tcggtgatct cgacgatgca gtcgacgatg
     5641 tcggggatct tggcctcggt ggcaccgtag gagagccgga tgtcggcgtc gatgccttgc
     5701 ttctcgaagt agcgccgggt gacctcgggg tactcggtgg agacccgcac gccctggggc
     5761 aggtcatcga cggactggac gtcggagtca ccgccgaccg ccacgaccat cttgatgggg
     5821 cgggccgtgg ccttgctgta gtggagctca ccgagggtca cgacctcact ggaggtctcc
     5881 tcgatccagt cccggccggt gatgccgagg tcgaacatgc cctcggccac gtagcgg
//
LOCUS       DASTFV010000085         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_295683, whole genome shotgun sequence.
ACCESSION   DASTFV010000085 DASTFV010000000
VERSION     DASTFV010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295683"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFP02_01560"
     CDS             <1..195
                     /locus_tag="VFP02_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HET9541738.1"
                     /translation="VTAWEGTAGTVVLEGSFWNAVGPPGLTDGDEVVVSGYEGMTLTV
                     RRAAPDPGRMPMTMPRKDVP"
     gene            195..1163
                     /locus_tag="VFP02_01565"
     CDS             195..1163
                     /locus_tag="VFP02_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HET9541739.1"
                     /translation="MAAAIAVLLVLVLVIVLILSSSVKIVLEYERAVIFRLGRCVGAK
                     GPGVFFIIPIIDKIRRVNLQIAAVPVASQAVITKDNVTITVDAVAYFQVVDPLASVIK
                     IQNWYNSAQLVAQTTLRSIIGRHELDQMLSERDRINSELQIALDTQTEAWGIKVHRVE
                     VRDVGLPEQMQRAMARQAEAERERRAKIIAAEGELQASQKLGEAADVINRSPGALQLR
                     QLQTMVEISAENTSTIIFPIPIEIMGAISALTTHLGGDPHPPIIPPAPAVDETEVGSS
                     VEATNALADVVIPPLDIPEPELPPSPTAPPSPDLPPPPTGPPPPTA"
     gene            complement(1164..2006)
                     /locus_tag="VFP02_01570"
     CDS             complement(1164..2006)
                     /locus_tag="VFP02_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9541740.1"
                     /translation="MELLFCGVRGSTPAPGADFVRVGGHTSCVALTRSGTDTPSLVLD
                     AGTGLQRLSDRLDGGPFRGTLLLTHLHWDHAQGLPFFRSGDQPDAEVRLLIPDQQDGR
                     AAVDVLAGSMSPPHFPIRPEGLEGHWTFATLEEGEHIIEGFEVTAVELPHKGGRTFGY
                     RIVDPGGATVVYASDHGPLALGPGEDGHGPIHEAIVDLAREADVLIHDAQFVAAELAV
                     AEAYGHATVDYCYRLAEKAGVRKLVLFHHSPVRVDDDVDRLGDLAPPGLDVTIAREGE
                     TLTL"
     gene            complement(2017..>2373)
                     /locus_tag="VFP02_01575"
     CDS             complement(2017..>2373)
                     /locus_tag="VFP02_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9541741.1"
                     /translation="VTAVETALERELATTIMRGGEKPAVRTVEPGGLLTEQGQAGHEV
                     FLLLDGVVDVAVDGEVLAEFGPGAILGERAVLEGGLRTSTITARTRCRVAAATGDQVD
                     HEALAELSEGHRKEDG"
BASE COUNT          351 a          870 c          821 g          331 t
ORIGIN      
        1 gtcaccgcct gggagggcac ggccggaacc gtggtcctcg agggctcgtt ctggaatgcc
       61 gttgggcccc cggggctgac cgatggtgac gaggtcgtcg tcagcggcta cgaaggcatg
      121 acgctgacgg tgcgccgagc ggcgcccgac ccgggaagga tgcccatgac catgccccgc
      181 aaggacgtgc cctgatggcc gctgccatcg cggtgctcct ggtcctcgtc ctcgtcatcg
      241 tgctgatcct ctcctcctcg gtgaagatcg tgctggagta cgagcgagcg gtcatcttcc
      301 gcctcgggcg gtgcgtgggg gccaagggtc ccggcgtgtt cttcatcatc ccgatcatcg
      361 acaagatccg gcgggtcaac ctccagatcg cggcggtgcc ggtcgcctcg caggccgtga
      421 tcaccaagga caacgtgacg atcaccgtcg acgccgtggc ctacttccag gtcgtcgatc
      481 cgctggcctc cgtcatcaag atccagaact ggtacaactc ggcccagctc gtggcccaga
      541 ccaccctgcg ctcgatcatc ggccgccacg agctggacca gatgctctcc gagcgggacc
      601 ggatcaactc cgagctgcag atcgccctgg acacccagac cgaggcgtgg ggcatcaagg
      661 tccaccgggt cgaagtccgc gacgtcggcc tgcccgagca gatgcagcgg gccatggccc
      721 gtcaggccga agccgagcgg gagcggcggg ccaagatcat cgccgccgag ggtgagctcc
      781 aggcctccca gaagctgggc gaagcggccg acgtgatcaa ccgctcgccc ggtgccctcc
      841 agctgcgcca gctgcagacg atggtcgaga tctccgccga gaacacctcc acgatcatct
      901 tcccgatccc catcgagatc atgggcgcca tctcggcgct gaccacccac ctcggcgggg
      961 atccccaccc cccgatcatc ccaccggccc cggctgtgga cgagaccgag gtgggttcat
     1021 cggtcgaggc gacgaacgcc ctcgccgacg tggtgatccc gccactcgac atccccgagc
     1081 ccgagctccc gccgtcgccg accgccccgc cctcgcccga tctgccgccg ccgcccacgg
     1141 gcccgccgcc gccgaccgcc tgatcagagg gtgagggtct cgccctcccg ggcgatggtc
     1201 acgtccaggc cgggaggagc gaggtcgccc aggcggtcga cgtcgtcgtc gacccgcacc
     1261 gggctgtggt ggaacaggac cagcttccgc acgccggcct tctcggccag gcggtagcag
     1321 tagtccaccg tggcgtggcc gtacgcctcg gccacggcca gctcggcggc gacgaactgc
     1381 gcgtcgtgga tgaggacgtc ggcctcgcgg gcgaggtcga cgatcgcctc gtggatcggg
     1441 ccgtggccgt cctcgcccgg gccgagggcg agtggaccgt ggtccgaggc gtagaccacc
     1501 gtcgccccac cgggatccac gatgcggtag ccgaaggtgc ggccgccctt gtggggcaac
     1561 tcgacggcgg tgacctcgaa gccctcgatg atgtgctcgc cctcctccag ggtggcgaag
     1621 gtccagtgcc cctccaggcc ctcgggccgg atcgggaagt gtgggggcga catcgagccg
     1681 gccaacacgt cgaccgcggc ccggccgtcc tgctgatcgg ggatgaggag ccggacctcg
     1741 gcgtcgggct ggtcgccgga gcggaagaag ggcaggccct gggcgtggtc ccagtgcagg
     1801 tgggtcagga gcagcgtgcc ccggaaggga ccgccgtcga gacggtcgga cagccgctgc
     1861 agcccggtgc cggcgtcgag caccagcgac ggggtgtccg tccccgagcg cgtgagcgcg
     1921 acacaggagg tgtggccccc gacgcgcacg aagtcggcgc ccggggccgg cgtcgaccca
     1981 cggacaccgc agaagaggag ttccactgct ggaggtctac ccgtcttcct tccggtgtcc
     2041 ctcactcagc tcggccagcg cctcgtgatc gacctggtcg cccgtggcgg cggcgacccg
     2101 gcagcgggta cgggccgtga tcgtcgaggt gcggagcccg ccctccagca ccgcccgctc
     2161 gcccaggatg gcgccggggc cgaactcggc caggacctcg ccgtcgacgg caacgtccac
     2221 cacgccgtcc aggagcagga acacctcgtg gccggcctgg ccctgctcgg tcagcaggcc
     2281 gcccggctcg accgtgcgca ccgccggctt ctcgccgccc cgcatgatcg tcgtggccag
     2341 ctcccgctcc agggcggtct cgaccgcggt gac
//
LOCUS       DASTFV010000086         2555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_300870, whole genome shotgun sequence.
ACCESSION   DASTFV010000086 DASTFV010000000
VERSION     DASTFV010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300870"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VFP02_01580"
     CDS             <1..459
                     /locus_tag="VFP02_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541742.1"
                     /translation="PATQIKSTFPVLKNPANHHKAVGYSLEQWTYAFTNTFTPERSRE
                     TYERYHVPASGRILWDSVLANLEPGHQGTWVDYDNDDRAPLLFISGDADNLMPPRIQQ
                     SNAKHYKSDTVTEVKEFAGPHLLPMAEGWEAVADYALDWALEHAVVSETA"
     gene            456..1253
                     /locus_tag="VFP02_01585"
     CDS             456..1253
                     /locus_tag="VFP02_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9541743.1"
                     /translation="MSDLRITHIGGPTVLVEVEGWRILSDPTFDPPGRTYAFGWGTSS
                     RKTAGPAVAPEDIGPVDVVLVSHDHHGDNLDDAGRALLPSAGVVVTNSSAAARLDGTT
                     RGLHAWETTWVEGVGRPTLEITATPCRHGPPLSRPIVGHVVGFALRWQGQPHGVLWFS
                     GDTVLYDGVREVAGRLDVDTAVVHLGEVKFPVTGPIRYSMTAEEGVELCGLLSPGTAI
                     PVHYEGWSHFQEGRAEVERAIAAASPDIRSRFRLLDLGVPAEIASSG"
     gene            complement(1272..1976)
                     /locus_tag="VFP02_01590"
     CDS             complement(1272..1976)
                     /locus_tag="VFP02_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9541744.1"
                     /translation="MARTHSRQTIRYERRTRDVFSVGVGLGVLGVGMLLVRDGNVSGA
                     EEAVFRAVNDLPEALYPVLWPFQQAGALFIGPLVALVALLLRRYRLAWAALATTVLKL
                     VSERAVKGVASRQRPGTSIGSDIHLRGDVSAGGESFVSGHAVLIAALATIVSPYLPGR
                     WKIVPWAIAGTVLFTRVYVGAHNPLDVICGAGLGLAIGGVLNLAFGVPAASRTPTPAE
                     PQTEPTPQPSTSPGTA"
     gene            2113..>2555
                     /locus_tag="VFP02_01595"
     CDS             2113..>2555
                     /locus_tag="VFP02_01595"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9541745.1"
                     /translation="MRRRRTIGFLVLAALGGVVVGCSSEPADQADSAAVRVASTLRDD
                     TITVGSFDFAESELVAEIYSQALEGDGYHVRRAFGLGPREFVLPALAGGLVEVVPEYA
                     GSALQFSSLASDIGTADVAATNDQLAELLAERGITVLAPSPAQDA"
BASE COUNT          384 a          915 c          897 g          359 t
ORIGIN      
        1 ccggccacgc agatcaagtc caccttcccg gtgctcaaga acccggccaa ccaccacaag
       61 gcggtgggct actcactgga gcagtggacc tacgccttca ccaacacctt cacccccgaa
      121 cggtcccggg agacctacga gcgctaccac gtgcccgcgt cgggccgcat cctgtgggac
      181 agcgtgttgg ccaacctcga gcccggccac cagggaacct gggtcgacta cgacaacgac
      241 gaccgggccc cgctgctgtt catctccggc gacgcggaca acctgatgcc gccccgcatc
      301 cagcagtcga acgccaagca ctacaagtcg gacacggtca cggaggtgaa ggagttcgcc
      361 ggcccgcacc tgctgcccat ggccgagggg tgggaagcgg tcgccgacta cgcgctggac
      421 tgggccctcg agcacgcggt cgtgtccgaa acggcgtgag tgacctgcgc atcacccaca
      481 tcggcgggcc gacggtcctg gtcgaggtcg agggctggcg gatcctctcg gatcccacct
      541 tcgacccgcc gggccggacc tacgccttcg gctgggggac gagctcccgg aagacggcgg
      601 gcccggccgt cgccccggag gacatcgggc ctgtcgacgt ggtcctggtc agccacgacc
      661 accacggcga caacctcgat gacgccggcc gggcgctcct gccttcggcc ggcgtcgtcg
      721 tgacgaactc gtcggcggcc gctcgcctgg acggcacgac gagggggctt cacgcgtggg
      781 agaccacgtg ggtcgagggc gtgggccgac cgacgctaga gatcacggcc acgccgtgcc
      841 gccacggtcc tccgctcagc cggccgatcg tcggccacgt cgtcggcttt gcgctccggt
      901 ggcagggcca gccccacggc gtcctctggt tctcgggtga caccgtgctc tacgacgggg
      961 tgcgggaggt cgccggccgc ttggacgtcg acaccgccgt cgtccacctc ggcgaggtga
     1021 agttccccgt caccggcccg atccgttaca gcatgaccgc cgaggagggg gtggagctgt
     1081 gcggcctcct gtcccccgga accgccatcc ccgtgcacta cgaggggtgg tcccacttcc
     1141 aggagggacg ggccgaagtc gagcgggcga tcgccgccgc gtcccccgac atccggagcc
     1201 ggttccggct cctcgacctc ggcgtaccgg cggagatcgc ttcgagcggg tgaccggggt
     1261 cgaaccggcg ttcaggccgt gccgggactg gtcgacggtt gcggcgtcgg ttccgtctgg
     1321 ggctcggccg gagtcggcgt gcgagacgcc gcgggcacgc cgaaggcgag gttgaggaca
     1381 ccaccgatcg ccagacccaa cccggcgccg cagatgacgt cgagcgggtt gtgggccccc
     1441 acgtacaccc gcgtgaacag caccgtgccg gcgatcgccc acggcacgat cttccaccgc
     1501 ccggggagat acggcgagac gatggtggcg agcgcagcaa tgagcaccgc gtgcccggac
     1561 acgaagctct cgccgccggc ggagacgtca ccacggaggt ggatgtcgct gccgatcgag
     1621 gtccctggtc gttggcgtga ggccaccccc ttcacggccc gctcggacac cagcttgagc
     1681 accgtggtcg ccaacgcggc ccacgccagc cggtagcgac gcagcagcag ggcgacgagg
     1741 gcgacgagcg gcccgatgaa cagcgccccg gcctgctgga acggccacag cacggggtag
     1801 agcgcctccg ggaggtcgtt caccgctcgg aagaccgcct cctcggcccc cgagacgttg
     1861 ccgtcgcgca cgagcagcat cccgacgccg agcacgccga gtccgacgcc gacgctgaac
     1921 acgtcgcggg ttcggcgttc gtagcggatc gtctggcggc tgtgggtgcg ggccaccggc
     1981 ctccctccga gtggtggtcg agcggcaacg gcgaccttac gcctcgatcg tcgagcccgc
     2041 gggggttggc ttccacggac cctgtcgatg tggaaggctc cttcgacgac ccttcggagg
     2101 aggagcggga cgatgcgccg tcgtcgaacg atcggctttc tggtcctcgc ggcgctcggt
     2161 ggcgtcgtcg tcgggtgcag ttcggagccg gccgaccagg ccgattcggc cgcggtcagg
     2221 gtggcgtcga ccctccgcga cgacacgatc acggtcggat cgttcgactt cgccgagagc
     2281 gaactggtgg ccgagatcta cagccaggcc ttggaaggcg acggctacca cgtgcgtcgt
     2341 gccttcggcc tcgggccccg ggagttcgtg ctgccggcgc tcgctggtgg tctggtcgag
     2401 gtggtgccgg agtacgccgg gagtgctctc cagttctcga gcctggcctc cgacatcggc
     2461 accgcggacg tcgccgccac caacgaccag ctcgcggagc tcctcgccga gcgggggatc
     2521 accgtgctcg ccccctcgcc ggcgcaggac gccaa
//
LOCUS       DASTFV010000087         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_307968, whole genome shotgun sequence.
ACCESSION   DASTFV010000087 DASTFV010000000
VERSION     DASTFV010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307968"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VFP02_01600"
     CDS             complement(<1..691)
                     /locus_tag="VFP02_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9541746.1"
                     /translation="MRVRGVLAALAALLTIVVGVPFGAGGAEATPARDPVIIVAGTFA
                     GQPAASIFYAPMAARLRADGYQPYIFGLPTSGTQDIALTAQALNTYADQVRAQTGATR
                     VDLIGHSQGGLVARHYVKHLGGSSEVDALISLGAPHYGTAVANVAKLFGLGNCIGITS
                     CVQMSIGSTYLANLNAGDDTIGAVRYTNIVSANDEVVIPYRTGWLADDGNNVNVRVQS
                     PCFLRIVGHVGL"
     gene            781..>2381
                     /locus_tag="VFP02_01605"
     CDS             781..>2381
                     /locus_tag="VFP02_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET9541747.1"
                     /translation="MLTISGLSKAHGNRTLFRGVTFVLGDGRRVALVGANGVGKTTLL
                     EIVMGQQPADAGEVHRSADLQVGYLPQDKPVVSSGTVLDETLDGGGPLAELHDRLEDL
                     EARLTAGDQGVLEEYGDVQSRFEALGGYALQAEAERILGGLGFTPEDSRRPVRELSGG
                     WRMRVLLARLLLSDPDVLLLDEPTNHLDVDSVGWLEQYLANYRGAILFVSHDRDFIDG
                     VANRVIEMSHESATEYVGGFAEFVVEREANLERLEAAANRQAKQLAKTEQFIERFRYK
                     ATKARQVQSRVKALEKIERITVPDDEIIRARFAFAPPPRSSRVMAELHGVDIGYDDEP
                     PVLTGVDLVVERGMRIGLIGPNGAGKTTLLKVLLGELDPVAGRVVMGSNLEIASFEQH
                     QADALDGSKRVVEEFSSSVPEAAGRNRRTLLGSFGFSGDAADRRVSDLSGGERTRLAL
                     GKIMVEPHNLLVLDEPTNHLDLPSCDILEDALRAYDGTVLLVTHDRHLIRSVATSLIE
                     VRGGTATWHDGVDEDILTPRLDSGPG"
BASE COUNT          350 a          862 c          842 g          327 t
ORIGIN      
        1 ccagacccac gtggcccacg atccgcagga agcaggggga ctggacgcgg acgttgacgt
       61 tgttgccgtc gtcggccagc cacccggtgc ggtaggggat cacgacctcg tcgttggccg
      121 acacgatgtt cgtgtagcgg acggcgccga tggtgtcgtc ccccgcgttg aggttggcca
      181 ggtaggtcga gccgatcgac atctgcacgc aggaggtgat gccgatgcag ttgcccaggc
      241 cgaagagctt ggccacgttg gccaccgccg tcccgtagtg cggcgccccc aggctgatga
      301 gggcgtcgac ctcggacgaa ccgccgaggt gtttgacgta gtggcgggcg accaggccgc
      361 cctgggaatg gccgatgagg tcgacccggg tggccccggt ctgggcccgg acctggtcgg
      421 cgtacgtgtt gagcgcctgc gccgtgaggg cgatgtcctg ggtgcccgac gtcggcagtc
      481 cgaagatgta gggctggtag ccgtcggccc gcagccgagc cgccatcggg gcatagaaga
      541 tgctcgccgc gggctggccg gcgaaggtgc cggccacgat gatcaccggg tcccgggccg
      601 gggtcgcctc cgcccctccc gcgccgaacg gcacccccac gacgatggtc agcaacgccg
      661 ccagcgcggc gaggaccccc cggactcgca tgcccgcaaa gtactcgctg gcgcgccggc
      721 cgctccggtg agccgaatcc cccgcagggc ggacccgggc cgctcgtagc ctgaccggcc
      781 gtgctcacga tctccgggct gagcaaggcc cacggcaacc ggaccctgtt ccggggcgtc
      841 accttcgtcc tcggcgacgg gcgccgggtc gcgctcgtgg gcgccaacgg cgtgggcaag
      901 acgaccctgc tggagatcgt catgggccag cagccggccg acgccggtga ggtgcaccgc
      961 tcggccgacc tccaggtcgg ctacctcccc caggacaagc cggtcgtctc ctcggggacc
     1021 gtcctcgacg agaccctgga cgggggcggc cccctggccg agctccatga ccgcctggag
     1081 gacctcgagg cccggctcac cgcgggcgac cagggcgtgc tcgaggagta cggcgacgtc
     1141 cagtcccgct tcgaggccct cgggggctac gccctccagg ccgaggccga gcgcatcctc
     1201 ggcggcctcg gcttcacgcc ggaggactcc cgccgcccgg tccgcgagct ctcgggtggt
     1261 tggcgcatgc gggtcctcct ggcccggctg ttgctgagcg accccgacgt cctcctgctc
     1321 gacgagccca ccaaccacct cgacgtcgac tccgtcgggt ggctcgagca gtacctggcg
     1381 aactaccggg gcgccatcct cttcgtcagc cacgaccggg acttcatcga cggggtggcc
     1441 aaccgggtca tcgagatgag ccacgagtcg gccaccgagt acgtcggcgg cttcgccgag
     1501 ttcgtggtcg agcgggaggc caacctcgag cggctggagg cggcggccaa ccgccaggcc
     1561 aagcagctgg ccaagaccga gcagttcatc gagcgcttcc gctacaaggc gaccaaggcc
     1621 cgccaggtcc agagccgggt caaggcgctg gagaagatcg agcgcatcac ggtccccgac
     1681 gacgagatca tccgggcccg cttcgccttc gccccgccgc cccggtcgtc ccgggtcatg
     1741 gccgagctcc acggcgtcga catcggttac gacgacgagc cgccggtgct gaccggcgtc
     1801 gacctggtgg tcgagcgggg catgcgcatc ggcctgatcg ggcccaacgg ggcgggcaag
     1861 accaccctgc tcaaggtcct gctcggtgag ctggatccgg tggcgggccg cgtcgtcatg
     1921 ggcagcaacc tggagatcgc ctcgttcgag cagcatcagg ccgacgccct ggacgggtcc
     1981 aagcgggtgg tcgaggagtt ctccagcagc gtgccggagg ccgccggccg gaaccggcgc
     2041 acgctgctcg gcagcttcgg cttcagcggg gacgccgccg accgtcgggt cagcgacctg
     2101 tccggcggcg agcgcacccg gctggccctc ggcaagatca tggttgagcc ccacaacctg
     2161 ctggtcctcg acgagcccac gaaccacctc gacctgccca gctgcgacat cctcgaggac
     2221 gccctccggg cctacgacgg gaccgtcttg ttggtcaccc acgaccggca cctgatccgg
     2281 tcggtggcca cctccctcat cgaggtccgg ggcgggaccg cgacctggca cgacggggtc
     2341 gacgaggaca tcctcacccc ccgcctggac agcgggcccg g
//
LOCUS       DASTFV010000088         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_308774, whole genome shotgun sequence.
ACCESSION   DASTFV010000088 DASTFV010000000
VERSION     DASTFV010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308774"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="VFP02_01610"
     CDS             <1..1504
                     /locus_tag="VFP02_01610"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9541748.1"
                     /translation="FDAQVGWDPFMTSSSWHGVTTAMIGNCGMSFAPVAPGSEAFLAE
                     MMESVEDIPRDAILGGIPWDWTTHPQYLDSVQRLSPALNVVSLVGHCAIRHQVMGERS
                     LTDEEPTPKELVRMREIVAESIAGGAVGYSTSRILVHVVPDGRAVPGTYASNAEHLAL
                     ADGMNEAGGGIFQAVLDFETRAGNEFQLLRAMAERAGDVLFAIGPGNDESTGLGPVEL
                     WDGFLSDTRAEAGRITGYTMTRPSGSLMGLAQVPPVKGGRWREVMGLPTMEDRLAALR
                     DDATRATLVEEGRRKGMLYDPTHIHPLGQGDTPRYDVEGGQSLADLADAADVHPVELI
                     IDRLLESEGRELFNIWFFHRNRAAIGPLLALDHVYPGAGDAGAHAGQICDADAPTHFL
                     AHWCRDEGLLALPEAVHRLTAKAAGTLGLVDRGTLEVGAYADVNVFDPSGLQVGYPTY
                     VNDFPGGAGRLCVRAEGYAITLVNGVVVTEQGEHSGARPGRVLREFARGA"
     gene            1509..>2372
                     /locus_tag="VFP02_01615"
     CDS             1509..>2372
                     /locus_tag="VFP02_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9541749.1"
                     /translation="MRVGVVILPEHSWPEARRLWRRTEELGFDHAWTYDHLSWRSLRD
                     GPWHAAVPTLAAAAVVTDKIRLGTLVASPTFRHPVPFARELITLDDLSAGRFTLGIGA
                     GGFGWDATTLDREPWSTKERADRFTEFVSLTDLLLRQAVTTREGRWYSAYEARSAPGC
                     VQQPRLPFAIAAGGPRGMRLTAEVGQAWVTVGERDREGGAAPVDEGVATVAGQIEALE
                     SACAAVGRDPATLDRIVLSGLNLDSGLGSVGEFEDAIGRFEAVGTTDVVVHWPRPTDP
                     YQGDEAAFEKLF"
BASE COUNT          330 a          870 c          824 g          348 t
ORIGIN      
        1 cttcgacgcc caggtcggct gggacccgtt catgacctcc agctcgtggc acggcgtgac
       61 gacggcgatg atcggcaact gcggcatgtc cttcgccccg gtcgcccccg ggagcgaagc
      121 cttcctggcc gagatgatgg aaagcgtcga ggacatacct cgcgacgcca tcctcggggg
      181 gatcccctgg gactggacga cccatccgca gtacctcgac tcggtccagc ggctgtctcc
      241 cgcgctcaac gtcgtcagcc tcgtcggcca ttgcgccatc cgccaccagg tgatgggcga
      301 gcgctccctg accgacgagg agccgacgcc gaaggagctg gtgcggatgc gggagatcgt
      361 cgccgagtcg atcgccggtg gtgccgtggg gtactcgacc tcgcgcatcc tggtccacgt
      421 ggtccccgac gggcgggccg tcccgggtac ctatgcctcg aacgccgaac acctggccct
      481 ggccgacggc atgaacgagg ccggtggcgg gatcttccag gccgttctgg acttcgagac
      541 gcgagccggc aacgagttcc agctcctccg agccatggcc gagcgcgccg gcgacgtgtt
      601 gttcgccatc ggtccgggca atgacgagtc gaccgggctc ggtcccgtcg agctctggga
      661 cgggttcctg agcgacaccc gggccgaggc cggtcggatc acgggctaca cgatgacccg
      721 accctccggc agcctgatgg gcctggccca ggtcccgccc gtcaaaggcg ggcgctggcg
      781 ggaggtcatg ggcttgccca cgatggagga ccggctagcg gcccttcggg acgacgccac
      841 ccgagcgacc ctcgtcgagg agggacggcg caaggggatg ctctacgacc ccacgcacat
      901 ccatccgctc ggacagggcg acaccccacg gtacgacgtc gaaggcggcc agtccctcgc
      961 cgatctggcc gatgccgccg acgtgcaccc ggttgagctg atcatcgacc ggctgctcga
     1021 gagcgagggc cgcgagctgt tcaacatctg gttcttccac cgcaaccggg cggccatcgg
     1081 gcccctgctg gcgctcgacc acgtctatcc gggggcaggc gacgccgggg cccacgccgg
     1141 acagatctgc gatgcggacg cccccaccca cttcctggcc cactggtgcc gcgacgaagg
     1201 tctgctcgcc ttgcccgaag ccgtccaccg gctcacggcg aaggccgcgg gcacccttgg
     1261 cctggtcgac cgcggcaccc tcgaggtcgg cgcctacgcg gacgtgaacg tgttcgaccc
     1321 gtccggcctg caggtcggct accccaccta tgtcaacgac ttcccgggcg gcgcgggtcg
     1381 cctgtgcgtg cgggccgagg ggtacgccat caccctcgtc aacggcgtgg tcgtgaccga
     1441 gcagggcgag cactccggcg cccgcccggg ccgggtcctg cgcgagttcg cccggggagc
     1501 ctgagccggt gcgagtcggc gtggtcatcc tcccggagca ctcatggccg gaggcccgac
     1561 ggctctggcg gcggaccgag gagctgggct tcgaccacgc ctggacctat gaccacctga
     1621 gctggaggag cctgcgcgac ggcccatggc acgcggcggt gccgaccttg gcggcggccg
     1681 ccgtcgtgac cgacaagatc cggctcggga ccctcgtggc gtcccccacc ttccgtcacc
     1741 cggtgccctt cgcccgagag ctcatcaccc tcgacgatct ctcggccggg cgcttcaccc
     1801 tgggcatcgg tgccggcggc ttcggctggg acgccaccac gctcgaccgt gagccctggt
     1861 cgaccaagga gcgggcggat cggttcaccg agttcgtgtc cctcaccgac ctcctgctgc
     1921 gccaggcggt gacgacccgc gagggtcgtt ggtactcggc gtacgaggcc cgctccgcac
     1981 ccgggtgcgt ccagcagccc cgattgccct tcgccatcgc cgcgggcggc ccccggggca
     2041 tgcgcctcac ggccgaggtc ggccaggcct gggtcacggt cggcgagcgg gatcgggaag
     2101 gtggtgctgc gccggtcgac gagggcgtgg ccacggttgc cggccagatc gaggcgctcg
     2161 agtctgcctg tgcggccgtc ggccgggacc ccgcgaccct cgaccgcatc gtgctcagcg
     2221 gtctcaacct ggacagcggc ctcggctcgg tcggcgagtt cgaggacgcc atcggccgct
     2281 tcgaggccgt cggtaccacc gatgtcgtcg tgcactggcc ccgccccacc gacccctacc
     2341 agggcgacga ggcggccttc gagaagctgt tc
//
LOCUS       DASTFV010000089         2525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_314443, whole genome shotgun sequence.
ACCESSION   DASTFV010000089 DASTFV010000000
VERSION     DASTFV010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314443"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /gene="carB"
                     /locus_tag="VFP02_01620"
     CDS             complement(<1..778)
                     /gene="carB"
                     /locus_tag="VFP02_01620"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379077.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HET9541750.1"
                     /translation="MPRRDDIESILLIGSGPIVIGQACEFDYSGTQACRVLREEGYRV
                     ILANSNPATIMTDPDFADATYVEPLDLEILTRIIEREQPDALLPTLGGQTALNLAMAL
                     VEAGVLDEHGVELIGANAEAIATAEDRDQFKQAMIEIGLGVPNSAIAHTLEEALAVVD
                     EIGLPVIIRPAYILGGRGTGMAATPDEFAKAAKHGLESSPISEILIEQSIAGWKEFEL
                     EVMRDRADNCVVICSIENVDPMGVHTGDSITVAPAQTLSDV"
     gene            complement(778..1860)
                     /gene="carA"
                     /locus_tag="VFP02_01625"
     CDS             complement(778..1860)
                     /gene="carA"
                     /locus_tag="VFP02_01625"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET9541751.1"
                     /translation="MTGPIREAHLVLADGTTFEGEAIGAEPPEGVATGEVVFNTVLSG
                     YQEVITDPSYAGQMIAFTYPHIGNYGVTPEDDESAKPFCRGVIIRELARRRSNWRAEE
                     DLDAFLRRHGVPGIAGVDTRRLTRHIRDAGSMPGAFGTADETTLKQAAADEPGTDGQD
                     LASVVSCTEPYTVGDGPRRVVAYDLGMKATILRHLSGLATVEVVPAWTTADEVLAREP
                     DGVFLSNGPGDPAAVTTTTEAIRGLLGQVPVFGICLGHQLLATALGGATHKLPFGHHG
                     GNHPVQRLATGGIEITSQNHNYAVAPDSLGAAEVTHVNLNDGVIEGLRSNDVPAFSVQ
                     YHPEAGPGPHDARYLFALFADLMDDT"
     gene            complement(1857..>2525)
                     /locus_tag="VFP02_01630"
     CDS             complement(1857..>2525)
                     /locus_tag="VFP02_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9541752.1"
                     /translation="AAAEELMVARDLALARLTGARVHFQHMSTAGSVELIRRAKADGV
                     RATAEVAPHHLTLTDAEVAAFDAVFKVNPPLREQSDVDAVRAGLIDGTLDAVATDHAP
                     HTQEDKEKPFDSAPPGMLGLEYALAIVLTEVGLPVEQALAVLSWRPAAIARLTEEHGG
                     PVAEGRPANLCVVDPDAEWTVDPADSASRSRNNPYAGRKLTGRVRHTVYRGEAVVIDT
                     EPQR"
BASE COUNT          323 a          816 c          985 g          401 t
ORIGIN      
        1 cgacgtcgga cagggtctgg gccggcgcca ccgtgatcga gtcaccggtg tggacgccca
       61 tgggatccac gttctcgatg gagcagatga cgacgcagtt gtcggcccgg tcccgcatga
      121 cctcgagctc gaactccttc cacccggcga tggactgctc gatgaggatc tcggagatgg
      181 ggctcgactc gaggccgtgc ttggccgctt tggcgaactc gtcgggcgtc gccgccatgc
      241 ccgtcccccg gccgcccagg atgtaggcgg ggcggatgat gaccgggagg ccgatctcgt
      301 cgacgacggc cagggcctcc tcgagcgtgt gggcgatggc cgaattgggt acccccaggc
      361 cgatctcgat catcgcctgc ttgaactggt cccggtcctc cgcggtggcg atggcctcgg
      421 cattggcccc gatgagctcg accccgtgct cgtccagcac cccggcctcc accagggcca
      481 tggccaggtt cagcgccgtc tggccgccga gggtgggcag cagggcgtcg ggctgctccc
      541 gctcgatgat ccgggtgagg atctccaggt ccaggggctc gacgtaggtg gcgtcggcga
      601 agtccgggtc ggtcatgatc gtggccgggt tcgagttggc caggatgacc cgatagccct
      661 cctcccgcag gacccgacag gcctgggtcc ccgagtagtc gaactcgcac gcctggccga
      721 tgacgatcgg cccggagccg atcaggagga tggactcgat gtcgtcccga cggggcatca
      781 ggtgtcgtcc atgaggtcgg cgaagagggc gaacaggtag cgggcgtcgt gcgggccggg
      841 tccggcctcg gggtggtact ggacgctgaa ggcgggcacg tcgttgctgc gcaggccctc
      901 gatcaccccg tcgttgaggt tcacgtgggt gacctcggcc gcgccgagcg agtcgggggc
      961 gacggcgtag ttgtggttct ggctggtgat ctcgatgcca ccggtggcca ggcgttggac
     1021 ggggtggttc ccgccgtggt ggccgaacgg cagcttgtgg gtggccccgc cgagggccgt
     1081 ggccaggagc tggtggccga ggcagatgcc gaagaccggg acctggccga ggaggccacg
     1141 gatggcctcg gtcgtggtgg tgaccgcggc cgggtcgccg gggccgttgg acaggaacac
     1201 cccgtcgggc tcccgggcca ggacctcgtc ggcggtcgtc caggcgggca cgacctcgac
     1261 cgtggccagt ccgctcaggt ggcgcaggat cgtggccttc atgcccaggt cgtaggccac
     1321 gacccggcgc gggccgtcgc ccaccgtgta gggctcggtg cagctgacga ccgaggccag
     1381 gtcctgaccg tcggtgccgg gctcgtcagc cgcggcctgc ttgagcgtgg tctcgtcggc
     1441 ggtgccgaag gcgccaggca tggagccggc gtctcggatg tggcgggtga ggcgacgggt
     1501 gtcgaccccc gcgatccccg gtacgccgtg gcggcgcagg aaggcgtcca ggtcctcctc
     1561 cgcccgccag ttgctgcggc gccgcgccag ctcccggatg atgacgcccc ggcagaaggg
     1621 cttggcgctc tcgtcgtcct cgggggtgac gccgtagttg ccgatgtgcg ggtacgtgaa
     1681 ggcgatcatc tgaccggcgt aggacgggtc ggtgatgacc tcctggtagc cggacaggac
     1741 cgtgttgaac acgacctccc ccgtggccac gccctcgggc ggctcggccc cgatggcctc
     1801 cccctcgaac gtcgtcccgt cggccaggac gagatgcgcc tctcggatcg gcccggtcat
     1861 cgctggggct cggtgtcgat gacgacggct tcgccgcggt agacggtgtg gcggacccgg
     1921 cccgtgagct ttcgaccggc gtaggggttg ttgcgggagc gactggcgga gtcggcgggg
     1981 tcgacggtcc actcggcgtc agggtcgacg acgcagaggt tggcggggcg gccctcggcg
     2041 acggggccac cgtgctcctc ggtgagacgg gcgatggccg cgggccgcca ggagaggacg
     2101 gcgagggcct gctcgactgg caggccgacc tcggtgagca cgatggcgag tgcgtactcg
     2161 aggccgagca tcccgggcgg ggcggagtcg aagggctttt ccttgtcctc ctgggtgtgg
     2221 ggggcgtggt cggtggcgac ggcgtcgagg gtgccgtcga tcagaccggc ccggacggcg
     2281 tcgacgtcgg actgctcgcg cagcggcggg ttcaccttga acaccgcgtc gaaagcggcc
     2341 acctcagcgt cggtcagggt gaggtggtgc ggggcgacct cggctgtggc gcggacgccg
     2401 tcggccttgg cccttcggat gagctcgacc gagcccgccg tgctcatgtg ctggaagtgc
     2461 acccgggctc cggtcaggcg ggccagcgcc aggtcgcggg cgaccatgag ctcctccgcc
     2521 gccgc
//
LOCUS       DASTFV010000090         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_315326, whole genome shotgun sequence.
ACCESSION   DASTFV010000090 DASTFV010000000
VERSION     DASTFV010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315326"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1003
                     /locus_tag="VFP02_01635"
     CDS             71..1003
                     /locus_tag="VFP02_01635"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET9541753.1"
                     /translation="MEISRPRIESNPLSDTRSRFVIEPLEPGFGYTLGNSLRRTLLSS
                     IPGAAVTSIKIDGVLHEFTSIQGVKEDVTDVILNVKELRLRSETNEPATMFLRSTGPG
                     DVTAADIVPPADIDVINPELHLATLNTKAKLEMELRVEKGRGYRPAERNKSASDEIGR
                     IPVDSIFSPVRRVSYTVEATRSGEMTNYDRLILDVETDGSLSPEDALASAGGTLRELV
                     SLFADLTGGGEGLSLGPAEQTGPLGANLDTPIEELDLSVRSYNCLKREGITTVGELVT
                     KSEADLLDIRNFGAKSIDEVREKLQELGLSLREE"
     gene            1008..1367
                     /gene="rplQ"
                     /locus_tag="VFP02_01640"
                     /pseudo
     CDS             1008..1367
                     /gene="rplQ"
                     /locus_tag="VFP02_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440437.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            2112..>2327
                     /locus_tag="VFP02_01645"
     CDS             2112..>2327
                     /locus_tag="VFP02_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541754.1"
                     /translation="MRLVVAYDGGPFSGFARQRDRRTVQGELEAVLSRLAMQPVTTVG
                     AGRTDAGVHARGQVVHADVTARLDPGRV"
BASE COUNT          353 a          885 c          815 g          274 t
ORIGIN      
        1 caagtagcga ccacgagggc ttccccgagt catatggcgg gccggggagc cggaggtgaa
       61 caccgtaccc atggagatct cgcgcccacg catcgagtcc aacccactgt cggacacccg
      121 ctcgcgcttc gtcatcgagc cgctggagcc gggcttcggc tacacgctgg gcaactcgct
      181 gcgccgcacc ctgctgtcgt ccatcccggg cgccgcggtc acatcgatca agatcgacgg
      241 cgtcctgcac gagttcacgt ccatccaggg cgtgaaggag gacgtcaccg acgtcatcct
      301 caacgtcaag gagctgcgcc tgcgctcgga gaccaacgag ccggcgacca tgttcctgcg
      361 ctccaccggc cccggtgacg tgaccgcggc cgacatcgtg cccccggccg acatcgacgt
      421 catcaacccc gagctgcacc tggccaccct gaacaccaag gccaagctgg agatggagct
      481 gcgggtcgag aagggccgcg gctaccggcc ggccgagcgc aacaagtcgg cctccgacga
      541 gatcggccgc atcccggtcg actcgatctt ctcgccggtg cgccgggtca gctacaccgt
      601 cgaggccacc cgctcgggcg agatgaccaa ctacgaccgg ctgatcctgg acgtcgagac
      661 cgacgggtcg ctgtcccccg aggacgcgct cgcctcggcc ggcggcaccc tgcgcgagct
      721 ggtcagcctg ttcgccgacc tgaccggcgg cggcgagggc ctctcgctcg gcccggccga
      781 gcagaccggc ccgctcggcg ccaacctgga cacgccgatc gaggagctcg acctgtcggt
      841 ccgctcctac aactgcctga agcgcgaggg catcaccacc gtcggcgagc tggtcaccaa
      901 gtccgaggcc gacctgctcg acatccgcaa cttcggcgcc aagtcgatcg acgaggtccg
      961 cgagaagctc caggagctcg gcctttcgct gagggaggaa tagtcgtatg ccgaccccga
     1021 ccaaggggcc tcgcctgggc gggggaccgg cccacgagaa gctgctcatc gccaacctgg
     1081 ccagggccct gttctccgag ggccggatcc gcaccaccga ggccaaggcc aagcgcctgc
     1141 ggccccaggc ggaacggctg atcaccttcg ccaagcgcgg cgacgtggcc agccgccgcc
     1201 aggtccttac cgtggtccgc gacaaggcgg tcgtgcacaa gctgttcgcc gagatcgccc
     1261 cgcggttcgc cgagcgccag ggcggctaca cccgcatcct caagctcggc ccccggccgg
     1321 gcgacgccgc ccccatggtc ctgatcgagc tggtggagca gggcgagggc gagcggaccg
     1381 gcgacaccga ggacggtggc cggcgccgcc gcttcggtcg ccgttcccgg gccgaggccg
     1441 cccccgaggg cgccaaccgg gccgagcgcc gcgccgccct gcgccgccgc ggccccgcgc
     1501 ccggcgaggc cgaggcggcc ctggccggcg aggacctcga cgacgacgcc gacgaggtcg
     1561 acgaggagcg cccggccgtc cccaccgacg ccgagatcga agccggcgtc accgccgccg
     1621 gcgacgagga cgaggacgag accgccgacg aggtcgacga ggtcgatgag gtcgatgagg
     1681 ccacggccga ggagcccggc gtcaccgagg ccggcgtcga cgacaccgaa gacgcggccg
     1741 cccaggccga cgacgaggcc gccgacgacg agaccgaggc atccgccgcc gaggacgagg
     1801 accaggatcg caagtaggcc atgggcgccg ccccaggccc caccgaccgc agcaccggcc
     1861 gccccgcccg ctccggcgcc cgcaggcgtc ctcctgcgcc cggcgtccaa cctcccgccc
     1921 cttccggtgg cgagagcccc ggtcgggagg gtagccgagc tggccggcgg ggcgtcgggg
     1981 agtttccaca gccccctcgg tcctcacggc cgccccggtc ctcacctcct ccctcgtcct
     2041 cacagccccc tcgatcctcg cggcctctcc ggtcctcacg ggcccctggt ccggctgggg
     2101 agctgcggcg gatgcggttg gtcgtggcct atgacggggg gccgttctcg gggtttgccc
     2161 ggcagcggga ccggcggacc gtgcaggggg agctggaggc ggtgctttcg cggctggcca
     2221 tgcagccggt gacgacggtc ggggcgggga ggaccgacgc cggggtgcat gctcgggggc
     2281 aggtcgtcca tgccgacgtg acggcgcggc tcgaccccgg acgggtc
//
LOCUS       DASTFV010000091         2377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_322430, whole genome shotgun sequence.
ACCESSION   DASTFV010000091 DASTFV010000000
VERSION     DASTFV010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322430"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VFP02_01650"
     CDS             complement(<1..628)
                     /locus_tag="VFP02_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9541755.1"
                     /translation="MTPENVVVVLLDSLNRHDLGCYGGTDFATPHLDRFASERATRFT
                     RHVTGSLPCMPARHDILCGALDFLWKPWGSIEAWERPVTGALRDEGVVTMLISDHPHL
                     FETGGENYHTDFFAWDYQRGHEGDAWRTASDPSWVGSPAVGASWFFDRLHGTDLGLER
                     GYDRSRTWFRSEEDFPGPRVMAAAADWLRTAAPHHDRWLLFVDEFDPHE"
     gene            complement(701..1282)
                     /locus_tag="VFP02_01655"
     CDS             complement(701..1282)
                     /locus_tag="VFP02_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET9541756.1"
                     /translation="MSVTETTTTATRTVDGTEVPVAGTYVIDASHSHAGFVVRHLMVS
                     KVRGAFSGVSGTVEVGDDITASTVEVEIDPATVDTRDAGRDEHLRSADFFGTEEFGSI
                     TYTSSGIRRDGGGWVLDGDLTIKGVARPVSLDLEFLGAVTDPWGGSRLGFTAGGELDR
                     EDWGLNYNQALETGGVVIGKKVTLEIEAELVRQ"
     gene            1395..1871
                     /locus_tag="VFP02_01660"
     CDS             1395..1871
                     /locus_tag="VFP02_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9541757.1"
                     /translation="MACPSLDDPRITLAGLLFEVHDGLTTRLGAKRSPTSEVLIRLAR
                     SPERSLRMSDLAAQVTMSASGLSRAIDRLVATGSIERVACDYDRRVVHARLTDQGQAE
                     IEAMLPEHVRVLEESFSVLTPTERDRFEATLRKLRCQVNPEAVQASDPAPSRAAAS"
     gene            1940..2377
                     /locus_tag="VFP02_01665"
     CDS             1940..2377
                     /locus_tag="VFP02_01665"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET9541758.1"
                     /translation="MPFTHHVDVRYLEADQQGVVFNMWYLAYLDDAMTGLLAHGGLGY
                     PDLLAQGWDVQLVHTELDWVGPLRWGEPGEVDVALARVGTTSFTLQFAVRVGDRPVCT
                     AETVYVAVETDGWTKVPVPEVLLAALGPVSPLPPPRVVRPAGR"
BASE COUNT          342 a          827 c          848 g          360 t
ORIGIN      
        1 gctcatgcgg gtcgaactcg tcgacgaaga ggagccaccg gtcgtggtgg ggggcggcgg
       61 tgcgcagcca gtccgccgcg gccgccatga cccggggccc ggggaagtcc tcctcggacc
      121 ggaaccacgt ccgcgaccgg tcatagcccc gctccaggcc gaggtcggtc ccgtggaggc
      181 ggtcgaagaa ccagctggcg ccgaccgccg gcgagccgac ccagctgggg tcggacgcgg
      241 tccgccaggc atcgccctcg tggccccgtt ggtagtccca ggcgaagaag tcggtgtggt
      301 agttctcccc accggtctcg aacaggtggg ggtggtccga gatcagcatg gtgaccacgc
      361 cctcgtcgcg gagggcgccg gtgaccggcc gctcccaggc ctcgatcgag ccccagggct
      421 tccagaggaa gtcgagcgcg ccacagagga tgtcgtggcg ggccggcatg cagggcagtg
      481 acccggtcac gtggcgggtg aagcgggtgg cccgttcgga cgcgaaccgg tcgaggtggg
      541 gggtggcgaa gtcggtcccg ccatagcagc cgaggtcgtg ccggttcagc gagtcgagca
      601 ggacgaccac gacgttctcc ggagtcaccg ccccatgttc ccagacgcgc cacggcggcc
      661 cgtccggggg ggttgaacgg gccgccgcag agcgggtgag ctactggcgg accagctcgg
      721 cctcgatctc cagggtgacc ttcttgccga tgaccacgcc accggtctcg agcgcctggt
      781 tgtagttcag gccccagtcc tcacggtcca gctcgccacc ggcggtgaag ccaaggcgac
      841 tgccgcccca cgggtcggtc acggcgccga ggaactcgag gtccaagctc accggacggg
      901 ccacgccctt gatggtgagg tcaccgtcga gcacccagcc gccgccgtcc cggcggattc
      961 cggagctggt gtaggtgatg gagccgaact cctcggtgcc gaagaagtcg gcggagcgca
     1021 ggtgctcgtc ccgaccggcg tcacgggtgt cgacggtggc gggatcgatc tcgacctcga
     1081 ccgtggaggc ggtgatgtcg tcgcccacct cgaccgtgcc ggacacgccg ctgaaggcgc
     1141 cgcggacctt ggaaaccatc aggtggcgga cgacgaagcc ggcgtggctg tgggaggcgt
     1201 cgatgacgta ggtgccggcg acagggactt cggtgccgtc gaccgtccgg gtggcggtgg
     1261 tggtggtctc ggtgacgctc atggtgtgct cctcatgggg gttctggttg tttctgcaat
     1321 agttgctgac gcaatcactg tacgtgcaag aacttgcgcg tgcaaccttt tgttcccgca
     1381 tctaccatcg ggtcatggcc tgtccgtccc tcgacgatcc ccggatcacc ctcgccgggc
     1441 tcctcttcga ggtgcacgac ggcctgacca cccgactggg cgccaagcgc agcccgacca
     1501 gcgaggtgct gatccgcctg gcccggagtc ccgagcggag cctgcgcatg agcgacctgg
     1561 ccgcccaggt gaccatgtcg gccagcggcc tgagtcgggc catcgaccgt ctggtggcca
     1621 ccgggtcgat cgagcgggtg gcgtgtgact atgaccgccg cgtcgtccac gcccgcctca
     1681 ccgaccaggg tcaggccgag atcgaggcca tgctccccga gcacgtccgg gtcctcgagg
     1741 agtccttctc ggtcctcacc cccaccgagc gcgaccggtt cgaggccacc ctgcgcaagc
     1801 tccgctgcca ggtgaacccc gaggccgtcc aggccagcga cccggcaccc agccgggccg
     1861 ccgcctccta ggacgacccg ctcccggctc tggcggccga tccgaccgaa cggatcggcg
     1921 cccacctaga ttcgcggcgg tgccgttcac ccaccacgtc gacgtgcgct acctggaggc
     1981 ggaccagcag ggcgtggtgt tcaacatgtg gtacctggcc tacctggacg atgccatgac
     2041 cggcctgctg gcccacggcg ggctcggcta ccccgacctc ctggcccagg ggtgggacgt
     2101 ccagctcgtc cacaccgagt tggattgggt cgggccgctg cgctggggcg agccgggcga
     2161 ggtcgacgtc gcgttggccc gcgtcgggac cacgtcgttc accctgcagt tcgccgtccg
     2221 ggtgggtgac cgccccgtgt gcacggccga gaccgtttac gtcgccgtag agaccgacgg
     2281 ctggacgaag gtgccggtgc ccgaggtcct gctcgccgcc ctcggccccg tcagcccgct
     2341 cccgccccct cgagtggtca ggccagcggg gcgatga
//
LOCUS       DASTFV010000092         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_323693, whole genome shotgun sequence.
ACCESSION   DASTFV010000092 DASTFV010000000
VERSION     DASTFV010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323693"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VFP02_01670"
     CDS             complement(<1..840)
                     /locus_tag="VFP02_01670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9541759.1"
                     /translation="MEFAFSEDQLAFRDAVRDLLAGTCPPEAVRTAWDADEPWDGDRW
                     DALVEMGVLGLTVPEADGGLGRTEVDLVLLLEEAGRAALPEPLMESALLARDVPGGGS
                     AKVAAAPADATHVAGAASADVVVVGSTVYPAPTGVQPQPSVDRARRLVRSVDVTAAEP
                     LATVDRNPFERMAFGTAATLVGVARRLVEVTVDYAKERQQFGQPIGGFQAVKHHLASA
                     HLAVEFAAPAVYRAAWSLATDQPAVARDVSMAKAMAGDAAEQAARVALQVHGAIGYTF
                     ESNL"
     gene            complement(911..2065)
                     /locus_tag="VFP02_01675"
     CDS             complement(911..2065)
                     /locus_tag="VFP02_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9541760.1"
                     /translation="MDLTFSDQHEGFRAEAREWLQANVPSGLGSMNTAAGFAAHRAWE
                     RTLFDARWAVVSWPEEYGGRGADLIEWLIFEEEYWAAGGPGRVTQNGIFLLAPTIFEH
                     GTKEQQDRYLPRMAAGEELWAQGWSEPDAGSDLAGLRSRAVQDGDDWVLTGQKTWSTR
                     GVFCDWLFGLFRSDPEAQRHQGLTYFLVPLDAEGVTVRPISGLHDDVAFAEVFLEEVR
                     VPSANVLGGVNEGWRVAMATASSERGLTLRSPGRFMAAADRLIDLYRRRPDEHLRDDV
                     ARAWMNAEAYRLLTFQAVSRMLQTGQPMGAEASLMKLWWSELDVHLNQTAQRLVGADA
                     ELGLDSEDAPDDGRWLDGWLFSLAGPIYAGTNEIQRNVVAERVLGLPRVR"
BASE COUNT          325 a          788 c          767 g          292 t
ORIGIN      
        1 gaggttcgac tcgaacgtgt agccgatggc accgtggacc tggagggcga cccgggcggc
       61 ttgctcggcc gcgtcaccgg ccatggcttt ggccatcgag acatcgcggg cgacggcggg
      121 ctggtcggtg gccagcgacc aggcggcccg gtagacggcc ggggcggcga actcgaccgc
      181 caggtgggcc gacgccaggt ggtgcttcac ggcctggaac ccgccgatgg gctggccgaa
      241 ctgctggcgc tccttggcgt agtcgacggt gacctcgacg agccgccggg ctacgccaac
      301 aagggtggcc gcggtgccga aggccatccg ctcgaacggg ttgcggtcga cggtggcgag
      361 aggctcagcc gccgtcacgt cgaccgagcg gacgaggcgg cgggcccggt cgaccgaggg
      421 ctggggctgg acgccggtcg gcgccgggta gacggtcgag cccacgacca cgacgtcggc
      481 ggaggcggcc ccggccacgt gggtggcgtc ggcgggcgcg gcggccacct tggccgaacc
      541 ccctcctgga acgtcccgag ccagcagggc cgactccatg agcggttcgg gcagggccgc
      601 ccgacccgct tcctccaaca ggaggaccaa gtcgacctcg gtcctgccca gcccaccgtc
      661 ggcctccggc acggtcaggc ccaggacgcc catctcgacc agggcgtccc accggtcccc
      721 gtcccacggt tcgtccgcgt cccaggccgt ccgcaccgct tctggcgggc acgtcccggc
      781 cagcagatcc cgcaccgcat cccgaaaggc gagctggtcc tcggagaagg cgaactccat
      841 cagctgaccc cccgttctgg aacctgaaac tcgcggccaa tcacaaaccg caggttccag
      901 cacggtgagg tcatcggacc ctcgggagac cgaggacgcg ttcggcgacg acgttgcgct
      961 ggatctcgtt ggtgccggcg tagatgggac cggccagcga gaagagccag ccgtccagcc
     1021 agcggccgtc atcgggggcg tcctcgctgt cgaggcccag ctcggcgtcg gcgccgacca
     1081 ggcgttgggc cgtctggttc aggtggacgt cgagctcgga ccaccagagc ttcatcaggc
     1141 tggcctcggc tcccatgggc tggccggtct gcagcatgcg gctgacggcc tggaaggtca
     1201 ggagccggta ggcctcggcg ttcatccagg cccgggccac gtcgtcccgc aggtgctcgt
     1261 cgggccggcg ccggtagaga tcgatcaggc ggtcagcggc cgccatgaac cgaccggggc
     1321 tgcgcagggt caggcctcgc tcggaggacg ccgtggccat ggccacccgc cagccctcat
     1381 tgaccccgcc gaggacattg gccgacggca cccggacctc ctccagaaag acctcggcga
     1441 aggccacgtc gtcgtgcagg ccactgatgg ggcgcacggt cacgccctcg gcgtccagcg
     1501 ggacgaggaa gtaggtcagg ccctggtggc gctgggcctc ggggtccgag cggaacaggc
     1561 cgaagagcca gtcgcagaac acgcctcggg tcgaccatgt cttctgcccg gtcagcaccc
     1621 agtcgtcgcc gtcctggacg gcccggctgc gcaggccggc caggtcgctg ccggcgtcgg
     1681 gctcggacca gccctgggcc cacagctcct cccccgccgc catcctgggc aggtagcggt
     1741 cctgctgctc tttggtcccg tgctcgaaga tcgtcggcgc cagcaggaag atgccgttct
     1801 gggtgacccg gccggggccg cccgccgccc agtactcctc ctcgaagatc agccactcga
     1861 tgaggtcggc gccccgaccg ccgtactcct caggccacga cacgacggcc cagcgggcgt
     1921 cgaacagcgt ccgttcccac gccctgtggg ctgcgaatcc cgcggcggtg ttcatcgacc
     1981 ccagccccga cggcacgttg gcctgcaacc attcccgcgc ctcggcccga aagccttcgt
     2041 gctggtcgga gaaggtcagg tccatgccgg cgagagcctg acgccccgtc cgaccgagtc
     2101 gcaactcgga cctgacgacc cgtcagatgg ggcgagtacc ctccgagccg tgagctcgac
     2161 ggacacggcg aa
//
LOCUS       DASTFV010000093         3020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_325338, whole genome shotgun sequence.
ACCESSION   DASTFV010000093 DASTFV010000000
VERSION     DASTFV010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325338"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VFP02_01680"
     CDS             complement(<1..585)
                     /locus_tag="VFP02_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET9541761.1"
                     /translation="MTHRLLATVGAIAVLAALTTACVPGPPEDIAGATEGDDPVTIAR
                     ESVLRIRAVGACGVAIGSGFVVDGRHLVTNRHVVAGASRLQVETWDGRRVAVGDAHQA
                     VDTDLGVIDLAARSARRLEPLALADEPTPVGARLTALGYAMAGPAVSTQGQFLDRPRG
                     RRFEEPARVMRMSASVRPGNSGGPLLNADGEVVGV"
     gene            complement(582..1295)
                     /locus_tag="VFP02_01685"
     CDS             complement(582..1295)
                     /locus_tag="VFP02_01685"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HET9541762.1"
                     /translation="MSSTETTSATGALPAAWYRDPSGRHERRWWDGDHWTDRVADGDL
                     EAADPPVRAQPAVPELTAGVAPGAIEAHSAAVAPPETETQPGPGRRPPAEREVHGRGI
                     LALGIVGILAATASFLWGMQNHRTASEWRDRGEVLQEELATRASNTDALEEALSQSAS
                     RGAQARDGQQSFAELRDAAVTTVDQVRACALDLNHVMIALQLGDDPSIPIEQANRSCE
                     EASLNGETLIRVLDEVAGS"
     gene            complement(1362..2174)
                     /locus_tag="VFP02_01690"
     CDS             complement(1362..2174)
                     /locus_tag="VFP02_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HET9541763.1"
                     /translation="MAYDHPDITSLLTLEPHGPDTFVGTGPEYPWGKRVYGGQVVAQA
                     LRAAASTVDGPYRAHSLHAYFIRPGDADEPIRFEVDRLRNGRSFVTREVVARQAGGAI
                     LDLSCSFQVDEDEADVQVGGLPDVDLPRAAREDDSWGPLIERQWGQRPGPGVARTWLR
                     VRGPLGEDPILHQCALAFASDDAPMEAALTHHPRWSGREEDFGRFMTASLDHAIWFHR
                     PVQGDAWHLYDMASEGIRGGRGMSYGRVLTTDGVHVATVTQEVLIRDLRANA"
     gene            complement(2187..2780)
                     /locus_tag="VFP02_01695"
     CDS             complement(2187..2780)
                     /locus_tag="VFP02_01695"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9541764.1"
                     /translation="MLRVLVVRHGQSAWNAVGRWQGQADPPLTELGLHQAAHAAQHVG
                     AVDAVVASDLERSLTTAAIIAELIGIGPVLVDQGFRERHAGEWQGLTKAEIAEGWPGY
                     LEDRRRPPAFEPDDDFQARIFAALDRVRESVPAGDVLVVAHAGVVYQAEAVLGVDFEP
                     LPNLGGRWVESDDGGPWGLGDRVVLVDPDEFTVPGVE"
BASE COUNT          368 a         1073 c         1169 g          410 t
ORIGIN      
        1 gacgccgacg acctcgccgt cggcgttcag caggggccca ccggagttgc cgggacggac
       61 gcttgcgctc atccgcatga cccgggcggg ctcttcgaag cggcgcccgc ggggtcggtc
      121 gaggaactgg ccctgggtgc tgaccgccgg gcccgccatg gcgtagccca gagcggtgag
      181 ccgggccccg acgggtgtcg gttcgtcggc cagggccaac ggctcgagcc ggcgcgcgct
      241 gcgggcggcc aggtcgatga cgccgaggtc ggtgtccacg gcctggtggg cgtcgcccac
      301 ggcgacccgc cggccgtccc acgtctccac ctgcagccgg ctggcgccgg cgacgacgtg
      361 ccggttggtg accaggtgcc ggccgtcgac gacgaaaccc gagccgatgg ccacgccgca
      421 ggccccgacc gcgcggatgc gcagcaccga ctcccgcgcg atcgtgaccg ggtcgtcgcc
      481 ctcggtggcg ccggcgatgt cctcgggcgg ccccgggaca cacgccgtgg tcagcgcggc
      541 caggacggcg atcgcaccga ccgtggccag gagccggtgg gtcacgagcc ggcgacctcg
      601 tccagcaccc ggatgagcgt ctcgccgttg agcgaggcct cctcgcagct ccggttggcc
      661 tgttcgatgg ggatgctcgg gtcgtcgccc agctgcaggg cgatcatgac gtggttgaga
      721 tcgagggcgc aggcccgcac ctggtcgacg gtggtgacgg cggcgtcccg cagctcggcg
      781 aaggactgct ggccgtcccg ggcctgggcg ccgcgactgg ccgactggct cagggcctcc
      841 tcgagggcgt cggtgttcga ggcgcgggtg gccaactcct cttgcagcac ctcaccccgg
      901 tcgcgccact cgctggcggt gcggtggttc tgcatgcccc acaggaacga ggctgtggcg
      961 gcgaggatcc cgacgatgcc gagggcgagg atccctcgcc cgtggacctc gcgctcggcc
     1021 ggcgggcggc gcccgggccc gggctgggtc tcggtctccg gcggggcgac cgccgcgctg
     1081 tgggcctcga tggcgcccgg ggcgacgccc gccgtcagtt cgggcaccgc cggctgggcc
     1141 cggaccggtg gatccgcggc ttctaggtcg ccgtcggcca cccggtcggt ccagtggtcg
     1201 ccgtcccacc agcgtcgttc gtggcgcccg gagggatccc ggtaccaggc ggcaggaagc
     1261 gcgcccgtcg ccgacgtcgt ctcggtgctg ctcacggtcc gctcctggtc cgccggtcgt
     1321 tgccgtctct gatcggccgg aggagggtgg gtgctgagcc gtcaggcgtt ggcgcggagg
     1381 tcgcggatca ggacctcctg cgtgacggtg gccacgtgga cgccgtcggt ggtgaggacc
     1441 cggccgtagg acatgccccg gccgccccgg atgccctcgg aggccatgtc gtagaggtgc
     1501 caggcgtcgc cctgcaccgg gcggtggaac cagatggcgt ggtcgaggct ggcggtcatg
     1561 aagcgcccga agtcctcctc acggcccgac cacctcgggt ggtgggtgag ggcggcctcc
     1621 atgggggcgt cgtcggaggc gaaggccaga gcgcactggt gcaggatcgg gtcctccccc
     1681 aaggggcccc gcacccgcag ccaggtccgg gccacccccg ggcccgggcg ctggccccac
     1741 tgccgctcga tgagcgggcc ccacgagtcg tcctcccgcg ccgctcgggg caggtccacg
     1801 tccggcaggc cgccgacctg gacgtcggcc tcgtcctcgt cgacctggaa cgagcacgac
     1861 aggtcgagga tggccccacc cgcctgccgg gccacgacct cccgggtgac gaagctgcgc
     1921 ccgttgcgga gacgatccac ctcgaaccgg atgggctcgt cggcgtcacc gggccggatg
     1981 aagtaggcgt gcagggaatg ggcccggtag gggccgtcga cggtcgatgc cgccgctcgc
     2041 aaggcctggg ccacgacctg gccgccgtac acgcgcttgc cccaggggta ctccgggccg
     2101 gtcccgacga acgtgtccgg gccgtggggc tccagggtca agaggctggt gatgtcgggg
     2161 tggtcgtagg ccatcgcgtc agctcctcac tcgactccgg ggaccgtgaa ctcgtccggg
     2221 tcgacgagca cgacccggtc accgaggccc caggggccgc cgtcatcgga ctcgacccaa
     2281 cgcccgccga ggttcggcag gggctcgaag tcgacgccga gcactgcctc cgcttggtag
     2341 acgacgccgg cgtgggcgac gacgaggacg tcgccggcgg ggacggactc gcgcacccgg
     2401 tcgagggcgg cgaagatccg ggcctggaag tcgtcgtcgg gctcgaaggc aggcggtcgg
     2461 cggcggtcct cgaggtaccc gggccaaccc tcggcgatct ccgccttggt caggccctgc
     2521 cactcgccgg cgtggcgctc ccggaagccc tggtcgacca ggacgggccc gatgccgatg
     2581 agctcggcga tgatcgccgc cgtggtcagg gaccgctcga ggtcgctggc caccacggcg
     2641 tcgacggcgc cgacgtgctg ggcggcgtgg gccgcctggt ggaggccgag ctcggtcagg
     2701 ggtgggtcgg cctgaccctg ccaccggccc acggcgttcc acgccgactg gccgtggcgg
     2761 acgacgagga ctcggagcac ggtcggggac cgtaggcgca cggcggctga actccttggt
     2821 cgagcggccg gttgcgtacc ctgcctggat ggcccaggtg cgacttttcg cgtccatgcg
     2881 ggagtcggcg ggccgttccc acgacgagct gccgggcacg acggtggctg ccgtcctcga
     2941 cgcggcggtg gagcgctacg gcgccgactt cgccgcccgg ctccaacagt gccgcgtgtg
     3001 ggtcaacggg gagccggtga
//
LOCUS       DASTFV010000094         3950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_328030, whole genome shotgun sequence.
ACCESSION   DASTFV010000094 DASTFV010000000
VERSION     DASTFV010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328030"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFP02_01700"
     CDS             <1..310
                     /locus_tag="VFP02_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541765.1"
                     /translation="ARAQAMADYAKAEELSLAESVAYADSASDLPMLEAVGFPVAVNP
                     ETRLATLARKRGWLVETFDKAPGGPRPPLPLAARPGGREGWLARGLDRMTPTSGVSGR
                     "
     gene            307..1476
                     /locus_tag="VFP02_01705"
     CDS             307..1476
                     /locus_tag="VFP02_01705"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9541766.1"
                     /translation="MKALLFERNLPRYAAARVAGAVVPGRGAAWGPLRLKDTDVPDLP
                     SPAWERVRPRLSGICGSDLATIDGRSSRYFEPIVSFPFVPGHEVVGERDDGSRVVLEA
                     VLGCAAREIHPPCDACAVGDVGRCERVAFGDLEPGLQTGFCCDTGGGWSTLLVAHREQ
                     LHAVADDLADEAAVMVEPAACAVRAALAAGEGGVQIVLGAGTLGLLTVAALRRFGTPG
                     TLIATARYPHQARLAKELGADVVVDPGEAHRAVRRATGAMVIGDTLTRGADAVVDCVG
                     SADTIATGIDVARAGGRVVLCGMPGTVSLDLTALWHKEIALVGAYAYGLEADGRRTFD
                     LAFELVQEADLGRLVSATYPLDRYQEALEHAAAAGPRGAVKIAFDLRNERERESL"
     gene            1473..3068
                     /locus_tag="VFP02_01710"
     CDS             1473..3068
                     /locus_tag="VFP02_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HET9541767.1"
                     /translation="MSRPGFVLEVDRSTPPVVFHHGDTLRLERLPVGRSRIVYPAEPL
                     EGLKDPDEAIRQALLDPLGDSAPLPELLTAGMKLTIAFDDISLPLPPMRRPDIRQRII
                     EAVLDMAAAAGVDDVHLIAALALHRRMTEAELRHAVGDRVYDAFAPDGLLYNFDAEDP
                     ENLSFLGTTDEGEEVEISKRAAESDLLVYVNINLVAMDGGHKSTATGLASYRSLRHHH
                     NVHTLQHSRSLMDRHNSALHHSNWRMGDVIRDAGVKIFQIETTLNNDTFGGDLPLSVL
                     QKREWEWTMADRASFIGLKAGLERMPASARRKVFNSWKAPYALTSVQAGEVGPVHEET
                     TRNVHAQQLVAVEGQTDVLTMGLPYICPYNVNSVMNPILVMCLGLGYFFNLYRGRPLV
                     REGGVLIMTHPTPWEFHPVHHPSYIDFFEQVLSETTDPVEIEAKYEKSFAEDEWYRHL
                     YRTSYAYHGVHPFYMWYWGSHALQHLGGVIIVGGDVKAVRRLGFKPASTLQDAFEMAT
                     DVVGPQPTVTHLHNPPILLADVT"
     gene            3065..>3950
                     /locus_tag="VFP02_01715"
     CDS             3065..>3950
                     /locus_tag="VFP02_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9541768.1"
                     /translation="MRVRFAPTRLVRALPFPYRAPAIPRGVEPPRTKPRKGGAYDTDW
                     ARRWPARATRLVLLNGVIKPAAKALADPQFRGLDRLSTLEGPAIFVANHHSHLDTPLL
                     LSSIPEPWRNKMFVGAAADYFFGSPVTSALSALVIGAIPIERSKVSRRGADQAADLID
                     DGWSLTLFPEGGRSPDGWGQEFRGGAAYLALRCEVPVVPVHIAGTGKILGKGMKYPKP
                     AHTRLTFGHPLAIEEGESSTHLNERIEAAVATLADESTTDWWEARRRVHAGTTPSLTG
                     PDAPTWRRAWALGDRKGRA"
BASE COUNT          556 a         1526 c         1316 g          552 t
ORIGIN      
        1 ggcccgagcc caggccatgg ctgactacgc caaggccgaa gagctgtcgc tggccgagtc
       61 cgtggcctac gccgactcgg cgtccgacct gccgatgctg gaggccgtcg ggttccccgt
      121 ggcggtgaac cccgagaccc gactggcgac cctggcccgc aaacggggct ggctggtgga
      181 gaccttcgac aaggcgcccg gcgggccgcg cccgcccctc cctctggcgg cgcgccccgg
      241 gggccgggag ggctggctgg cccgtggcct cgaccgcatg accccgacca gtggggtgtc
      301 cggacgatga aggcccttct gttcgagcgc aacctgcccc ggtacgccgc cgcccgggtg
      361 gcgggcgcgg tcgtgcccgg ccggggtgcg gcctggggcc cgctgcggct gaaggacacc
      421 gacgtgcccg acctgccctc gcctgcgtgg gagcgggtac ggccccgcct gtccggcatc
      481 tgcggctccg acctggcgac gatcgacggc cggagctcgc gctacttcga accgatcgtc
      541 tcgttcccgt tcgtgcccgg tcacgaggtc gtgggcgaac gggacgacgg atcccgggtc
      601 gtgctcgaag ccgtgctcgg ctgtgccgcc cgagaaatcc accccccgtg tgacgcctgc
      661 gccgtcggcg acgtggggcg ctgtgagcgg gtcgccttcg gcgacctcga gcccggcctg
      721 cagacgggct tctgctgcga caccgggggc ggctggtcga ccctgctggt ggcccaccgc
      781 gagcagctcc acgccgtggc cgacgatctc gccgacgagg ccgcggtcat ggtcgagccc
      841 gcagcctgcg ccgtgcgggc cgccctggcc gctggcgagg gcggcgttca gatcgtgctc
      901 ggggccggca cccttggcct gctgaccgtg gccgccctgc gccggttcgg cacgccgggc
      961 accctgatcg ccaccgcccg ctacccgcac caggctcgcc tggccaagga gctcggggcc
     1021 gatgtcgtcg tcgatcccgg cgaggcccac cgggccgtac gccgggccac cggcgccatg
     1081 gtgatcgggg acaccctgac ccggggggcc gacgccgtcg tggactgcgt gggcagtgcc
     1141 gacaccatcg ccaccggcat cgacgtcgcc cgcgcgggcg ggcgggtcgt cctctgcggc
     1201 atgcccggca cggtgtccct cgacctcacg gccctgtggc acaaggagat cgccctcgtc
     1261 ggggcctacg cctacggtct ggaggccgat ggccggcgca cgttcgacct cgccttcgag
     1321 ctggtccaag aggccgacct cggccgcctg gtgtcggcca cctaccccct cgatcgatac
     1381 caggaggcgt tggagcacgc cgcggctgcc ggtccccgag gggccgtcaa gatcgccttc
     1441 gacctccgca acgaacggga gcgtgaatcg ctgtgagcag gcccggattc gtcctcgagg
     1501 tcgatcgttc gaccccaccg gtggtgttcc accacggtga cacgctgcgt ctggagcgac
     1561 tgcccgtcgg ccgctcccgc atcgtctacc ccgccgagcc cctggagggc ctgaaggacc
     1621 ccgacgaggc catccgccag gccctgctcg acccgttggg cgactccgcc ccgctgcccg
     1681 agctcctgac ggcgggcatg aagctgacca tcgccttcga cgacatctcg ctgccgctgc
     1741 ccccgatgcg ccggcccgac atccgccagc gcatcatcga ggcggtcctg gacatggccg
     1801 ccgccgccgg ggtcgacgac gtccacctca tcgccgccct cgccctgcac cgccgcatga
     1861 ccgaggccga gctgcgccac gccgtgggtg accgggtcta cgacgccttc gcccccgacg
     1921 gcctcctcta caacttcgac gccgaggatc ccgagaacct gtcgttcctg ggcacgaccg
     1981 acgagggcga ggaggtggag atctcgaagc gggcggccga gagcgacctg ctcgtctacg
     2041 tcaacatcaa cctcgtggcc atggacggcg gccacaagag caccgccacc ggtctggcca
     2101 gctaccgcag cctgcgccac caccacaacg tccacaccct gcagcactcc cgctcgttga
     2161 tggatcggca caactcggcc ctgcaccact cgaactggcg catgggtgac gtcatccggg
     2221 acgccggggt gaagatcttc cagatcgaga ccacgctcaa caacgacacc ttcggcggcg
     2281 acctgcccct gtcggtcctg cagaagcggg aatgggagtg gacgatggcc gatcgggcct
     2341 cgttcatcgg cttgaaggcg ggcctggagc ggatgccggc cagcgcccgg cgcaaggtct
     2401 tcaactcgtg gaaggcgccg tacgccctga cctcggtgca ggcgggcgag gtcggcccgg
     2461 tccacgagga gacgacccgc aacgtccacg cccaacagct ggtggcggtc gagggccaga
     2521 ccgatgtgct cacgatgggg ctgccctaca tctgccccta caacgtgaac tcggtgatga
     2581 acccgatcct ggtcatgtgc ctgggcctcg ggtacttctt caacctctac cggggccggc
     2641 ccctggtccg ggagggcggg gtgctgatca tgacgcaccc gaccccctgg gagttccacc
     2701 ccgtccacca ccccagctac atcgacttct tcgagcaggt gctgtccgag acgaccgatc
     2761 cggtggagat cgaggccaag tacgagaagt ccttcgccga ggacgagtgg taccgccacc
     2821 tgtaccggac ctcgtacgcc taccacggcg tccacccctt ctacatgtgg tactggggca
     2881 gccacgccct ccagcacctc ggcggcgtga tcatcgtggg cggcgacgtc aaggctgtcc
     2941 gtcggctcgg cttcaagccc gcctcgaccc tgcaggacgc cttcgagatg gccactgacg
     3001 tggtcgggcc tcagcccacc gtgacccacc tccacaaccc gccgatcctc ctggctgacg
     3061 tgacgtgagg gtccggttcg cgcccacccg gctggtccgg gcgctgccgt tcccgtaccg
     3121 cgccccggcg atcccgcggg gcgtcgagcc gccgcggacc aagccccgca agggcggtgc
     3181 ctacgacacc gactgggccc gccgctggcc ggctcgagcc acccggctgg tcctgctcaa
     3241 cggcgtcatc aagccggcgg cgaaggcgct ggccgacccc cagttccggg gcctggaccg
     3301 gctgagcacg ctcgagggcc cggcgatctt cgtggccaac caccacagcc acctcgacac
     3361 cccgctcctc ctcagctcca tccccgagcc gtggcgcaac aagatgttcg tgggcgcggc
     3421 ggccgactac ttcttcggca gccccgtcac ctcggccctg tcggccctgg tgatcggcgc
     3481 catccccatc gagcgctcga aggtgagccg ccggggcgcg gaccaggccg ccgacctgat
     3541 cgacgacggc tggagcctga ccctgttccc cgaggggggc cgctcccccg acggctgggg
     3601 ccaggagttc cggggcggcg ccgcctacct cgccctccgc tgcgaggtgc ccgtcgtccc
     3661 ggtccacatc gccggcaccg gcaagatcct gggcaagggc atgaagtacc cgaagcccgc
     3721 ccacacccgc ctcaccttcg gccaccctct cgcgatcgag gagggcgagt ccagcaccca
     3781 cctcaacgag cgcatcgaag cagccgtcgc caccctcgcc gacgagtcca ccaccgactg
     3841 gtgggaggcc cgccggcggg tccacgccgg gaccacgccc tcgctgaccg gccccgacgc
     3901 ccccacctgg cgccgggcct gggccctcgg cgaccgcaag ggccgcgccc
//
LOCUS       DASTFV010000095         3113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_331004, whole genome shotgun sequence.
ACCESSION   DASTFV010000095 DASTFV010000000
VERSION     DASTFV010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331004"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFP02_01720"
     CDS             <1..480
                     /locus_tag="VFP02_01720"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET9541769.1"
                     /translation="HTTVIDTPTGLVEIEVDDDQLVGLNFVETSLADQDDPSGIADAL
                     RRYFDGDLDAIADLPVRFDTGTPFQQEVWRALQTIPVGETISYGELARRVGRPTGFRA
                     VGSANGRNPVAVVVPCHRVIAADGTLGGYSGGLDNKRWLLAHEGVVLAEDQRLPGLV"
     gene            complement(477..1667)
                     /locus_tag="VFP02_01725"
     CDS             complement(477..1667)
                     /locus_tag="VFP02_01725"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HET9541770.1"
                     /translation="MARPDSLSAAQARRIHLYAQGFGEKRPPGRVDRRHVRKVFDRLG
                     LIQIDSVNVLVRSHYLPLFSRLGPYDRSLLDRFAYRDHEAFEYWGHEASLIQARFQPL
                     LRWRMASEHRWAGMRTFAKERADLIDEVHGLVLAEGLTGSSDLGGRERKKEPWWDWNE
                     NKAALEHLFYEGRLGATRRENFERAYCAPALVIPADVLAAPTPSEREAMVGLLEHSAR
                     AHGVGTAKDLGDYFRLPIKAVRPLLEEMADDGIVDRVEVEGWKQPTYVHPDAHLPRWV
                     KAAALVSPFDSVMWERDRVERVFDFNYRIEIYVPKPKRVYGYYVLPFLLGDRYVARVD
                     LKADRAASALLVQSAFAEDGVDHLEVAERLAEELRTMAGWLDLDDVVVKDRGDLAPTL
                     QLFV"
     gene            complement(1720..2790)
                     /locus_tag="VFP02_01730"
     CDS             complement(1720..2790)
                     /locus_tag="VFP02_01730"
                     /inference="COORDINATES: protein motif:HMM:NF040257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541771.1"
                     /translation="MHRVVVWGTGNAGRPAIRAVAAHRDLALVGAVVANPDKVGRDAG
                     ELAFVDPLGVVATDDPARAVADDVDAVVYTATGDTRPDDALADLLTCLEAGKDVVSTS
                     FYPLLHPPSAPRDLLDAVGAACATGDSSVFVSGIDPGWALDILPALVSGVGAGITEIR
                     VQEVFNYALYDQPDVVRQIIGFGGPMDKTPLMLFDVSLEMVWAPMVRVLADMLGLTLD
                     DVRIQVERRPLERTIEVPGMGTFEAGTQGAFRFEVQGVVGGRPLLVVEHITRIDDDCA
                     PDWQQSTSPGGEHRVQLTGHPNLAVSVHGTEPGEPGAAGGGNASAANRIVNAIPAVIA
                     APAGPVSPLDLPPITGTAQLAV"
     gene            2854..>3113
                     /locus_tag="VFP02_01735"
     CDS             2854..>3113
                     /locus_tag="VFP02_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9541772.1"
                     /translation="MIARDPTELTVKQLDAAAMRALAHPLRMRMVGLLRAEGPATASG
                     LAERLGVSSGLTSYHLRALADAAFVEDDPDHATRGRERWWRAS"
BASE COUNT          462 a         1089 c         1134 g          428 t
ORIGIN      
        1 cacacgaccg tgatcgacac ccccaccggc ctcgtcgaga tcgaggtcga cgatgaccag
       61 ctggtcgggc tcaacttcgt cgagacgagc ctggccgatc aggacgaccc gtccggcatc
      121 gccgacgccc tccgtcgcta cttcgacggc gacctcgacg ccatcgccga cctgcccgtg
      181 cgcttcgaca cgggcacgcc gttccagcag gaggtgtggc gggcgctgca gaccattccg
      241 gtcggcgaga ccatcagcta cggcgagctg gcccgccgcg tcggccggcc caccggcttc
      301 cgggcggtgg ggtcggccaa cggccggaac cccgtcgccg tggtcgtccc ctgccaccgg
      361 gtgatcgccg cggacggcac gctcggcggc tattccggcg gactggacaa caagcgctgg
      421 ctcctggccc acgaaggcgt cgtcctggcc gaggaccagc gcctccccgg cctcgtctag
      481 acgaacagct gcagggtggg cgcgaggtcg ccccggtcct tgacgacgac atcgtcgagg
      541 tcgagccagc cggccatcgt ccgcagctcc tcggccaggc gttcggccac ctcgaggtgg
      601 tcgaccccgt cctcggcgaa cgccgactgc acgagcagcg ccgaggccgc ccggtcggcc
      661 ttgaggtcga cgcgggcgac atagcggtcg cccagcagga acggcagcac gtagtacccg
      721 tagacccgct tcggcttcgg cacgtagatc tcgatgcggt agttgaagtc gaagacccgt
      781 tcgacccggt cccgttccca catcaccgag tcgaagggcg acaccagggc cgccgccttc
      841 acccagcggg gcaggtgcgc gtcggggtgc acgtaggtgg gctgcttcca gccctcgacc
      901 tcgacccggt ccacgatccc gtcgtcggcc atctcctcca gcagggggcg gacggccttg
      961 atgggcagcc ggaagtagtc gcccaggtcc ttggccgtcc ccacgccgtg ggcccgggcc
     1021 gagtgctcca ggaggccgac catcgcctcc cgctccgacg gggtcggcgc ggcgagcacg
     1081 tcagcgggga tcaccagcgc gggagcgcaa taggcccgct cgaagttctc ccggcgagtg
     1141 gcgcccaatc ggccttcgta gaagaggtgc tccagcgccg ccttgttctc gttccagtcc
     1201 caccacggct ccttcttgcg ctcgcgcccg cccaggtcgc tggagccggt gagcccctcg
     1261 gccaggacga ggccatggac ctcgtcgatg aggtcggccc gctccttggc gaacgtccgc
     1321 atgccggccc accggtgctc gctggccatg cgccagcgca gcaggggctg gaagcgggcc
     1381 tggatgagcg acgcctcgtg cccccagtac tcgaaggcct cgtggtcccg gtaggcgaag
     1441 cggtcgagca gcgaccggtc gtagggaccg agccgggaga acagcggtag gtagtggctg
     1501 cggacgagga cgttgaccga gtcgatctgg atcaggccga ggcggtcgaa caccttgcgc
     1561 acgtgacgac ggtcgacccg ccccgggggg cgcttctcgc cgaagccctg ggcgtagagg
     1621 tggatccgcc gggcctgggc cgcggagagt gagtcgggtc gggccacggc cggaggctac
     1681 ggagcggtat caggggtgac gtggacggat ccggacaggt cagacggcga gctgcgccgt
     1741 gccggtgatc ggaggcagat cgagagggct gaccgggccg gcgggggcgg cgatgacggc
     1801 cgggatggcg ttgacgatcc ggttggccgc cgatgcgttg ccaccgcccg cggcaccggg
     1861 ctcgcccggc tcggtcccgt gcacgctgac ggccaggttc gggtggccgg tgagctggac
     1921 ccggtgctcg ccgccgggtg aggtcgactg ctgccagtcg ggggcgcagt cgtcgtcgat
     1981 gcgggtgatg tgctcgacga ccaggagggg acgaccgccg acgacgccct ggacctcgaa
     2041 gcggaaggcg ccctgggtcc cggcctcgaa ggtgcccatg ccggggacct cgatggtgcg
     2101 ctcgagcggg cgccgctcga cctggatgcg cacgtcgtcg agggtcaggc ccagcatgtc
     2161 ggccaggacc cgcaccatcg gggcccagac catctccagc gagacgtcga acagcatcag
     2221 cggggtcttg tccatggggc cgccgaagcc gatgatctgg cggaccacgt cgggctggtc
     2281 gtagagggcg tagttgaaga cctcctggac ccggatctcg gtgatcccgg cgccgacgcc
     2341 gctgaccagg gcaggaagga tgtccagggc ccagcccggg tcgatgccgg acacgaagac
     2401 cgacgagtcg ccggtggcgc aggccgcccc gaccgcgtcg agcaggtccc gtggggccga
     2461 cggtgggtgg agcaacgggt agaaggacgt ggacacgacg tccttgcccg cctccaggca
     2521 ggtcaggagg tcggccaggg cgtcgtcggg gcgggtgtcg ccggtggccg tgtagacgac
     2581 ggcgtcgacg tcgtcggcca cggctcgagc cgggtcatcg gtcgccacca ccccgagcgg
     2641 gtcgacgaag gccaactcgc cggcgtcgcg ccccaccttg tcggggttgg ccacgaccgc
     2701 cccgaccagc gccaggtccc ggtgggcggc caccgcccga atggccggcc gacccgcgtt
     2761 gcccgttccc cacacgacga ctcgatgcat cgccggacgg tagccggggg cctcgggttg
     2821 atcatgcaaa ggatctttgc aaagatcctt tgcatgatcg ccagggaccc gacggagctc
     2881 accgtcaaac agctcgatgc cgccgcgatg cgtgccctcg cccacccgct gcgcatgcgc
     2941 atggtcgggc ttctccgggc cgagggcccg gccaccgcga gtgggctggc cgagcgcctc
     3001 ggcgtcagca gcggcctgac cagctaccac ctgcgggcct tggccgacgc ggcgttcgtc
     3061 gaggacgatc ccgatcacgc cacaagaggc cgggagcgat ggtggcgagc ctc
//
LOCUS       DASTFV010000096         2808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_331981, whole genome shotgun sequence.
ACCESSION   DASTFV010000096 DASTFV010000000
VERSION     DASTFV010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331981"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFP02_01740"
     CDS             <1..531
                     /locus_tag="VFP02_01740"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET9541773.1"
                     /translation="SAVSARLLQDGRLLAVALVSAGLSREAYEFDDAPMPAVPPPEDC
                     EVLARPPFPIPIRDQIESRHAIGPQPFQPPGDAVTGGWMRMVDPEPTDVHTLVQMADG
                     WVPAIFGTVAERLGVPTVDLTVHLRQTPTPEDDGWLLVRFRTRVAAEGYLEEDGEIWG
                     RSGRLLAQSRQLAAIL"
     gene            534..800
                     /locus_tag="VFP02_01745"
     CDS             534..800
                     /locus_tag="VFP02_01745"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET9541774.1"
                     /translation="MRRRAVVSGRVQGVWYRDTARQEAERLGVAGSAANRLDGRVVLE
                     AEGEEAAVEAFLAWAAEGPPRARVDAVSVEAIEPTGEPGFTVTW"
     gene            complement(837..1481)
                     /locus_tag="VFP02_01750"
     CDS             complement(837..1481)
                     /locus_tag="VFP02_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET9541775.1"
                     /translation="MALPSRLLRWLAGAALTVLVVAACGPDDGRHLADPNPDLTAVPV
                     PTTTPVPVIDEDAPLQGVGPGGLTLSSPDFAPGATMPRSATCRGDRSPELEWTDPPRR
                     VTELALVVQDVDAEGVAQWVVTGIPPGTTRARLGEPPGGSDVRLNSAGVAAYSGPCPQ
                     DGFAHRIVFTLYALDQPLPVGAADPTSVVTAVQAASYGSASLIGRAAPPVTEDG"
     gene            1556..2527
                     /locus_tag="VFP02_01755"
     CDS             1556..2527
                     /locus_tag="VFP02_01755"
                     /inference="COORDINATES: protein motif:HMM:NF019603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9541776.1"
                     /translation="MTTIHHDDDGDLGVLAGQRVAVVGYGNQGRSWALNLRDSGLYVQ
                     VCVRADATREQAEADGFTAADIAAADDADVVCVLVPDDAIPTLALTPGPAALTVVASG
                     YTLAFDRFAPAGDIGMVAPRMLGPEVRRCYEEGGGFITAVGVHRDLTGTAQARTLAVA
                     KGIGGLRQGAIELTARQEAVLDLAVEQVLSPALTHVSNNFVGVMLEQGIPLEAVITEL
                     VLSGEVERTYRLLREEGFARQMAYHSPTSQYGQLSRRGRYDDLDVGTTMRELVDDITS
                     GRFADEWDAEQDTGHARLAALTATYAGPEVQAFEDEMRTRLGERAAG"
BASE COUNT          384 a          985 c         1076 g          363 t
ORIGIN      
        1 agcgcggtgt ccgcccgcct gctccaggac ggccgactgc tggccgtggc cctggtgtcg
       61 gcggggctct cgcgggaggc ctacgagttc gacgacgcgc cgatgcccgc ggtcccgccg
      121 ccggaggact gcgaggtcct ggcccgaccg ccgttcccca tcccgatccg ggaccagatc
      181 gagtcccgtc acgccatcgg cccgcagccc ttccaaccgc ccggcgacgc cgtgaccggc
      241 ggctggatgc gcatggtcga cccggagccg accgatgtcc acaccttggt ccagatggcc
      301 gacggctggg tgccggcgat cttcgggacc gtggccgagc gcctgggcgt gcccacggtg
      361 gacctcaccg tgcacctgcg ccagacacct acacccgagg acgacggttg gctcctcgtc
      421 cgcttccgga cccgggtggc ggccgagggt tacctcgagg aggacggcga gatctggggc
      481 cgatcggggc ggctgctggc ccagtcccgc cagctcgccg cgatcctgtg acggtgcgtc
      541 gccgagcggt cgtgtccggc cgggtgcagg gcgtctggta ccgggacacg gcccgccagg
      601 aggccgaacg cctcggcgtc gccggctcgg ccgcgaatcg gctcgacggc cgagtggtcc
      661 tggaggcgga gggtgaggag gccgcggtgg aggccttcct ggcctgggcg gccgagggac
      721 cacctagggc ccgggtcgac gccgtcagcg tcgaagcgat cgaacccacc ggcgagcccg
      781 ggttcacggt cacctggtag cggcaggagg tacggcggcg tgacgacggg ttcggttcag
      841 ccgtcctcgg tgacgggcgg ggccgcgcgg ccgatgaggc tggccgagcc gtaggaggcg
      901 gcctggacgg ccgtgaccac cgaggtcggg tcggcggccc cgacgggcag cggctggtcc
      961 agggcgtaga gggtgaacac gatgcggtga gcgaagccgt cctgtgggca ggggccggag
     1021 taggcggcga cgccggcgct gttgagccgc acgtccgagc cgccgggtgg ctccccgagg
     1081 cgcgcccggg tcgtgcccgg tgggatgccg gtgacgaccc actgggcgac accctcggcg
     1141 tcgacatcct ggacgaccag ggccagctcg gtcacccggc ggggcgggtc ggtccactcc
     1201 agctccgggg accggtcgcc acggcaggtc gcgctacggg gcatcgtggc cccgggcgcg
     1261 aagtccgggc tggacagggt caggccaccg gggccgacgc cctggagcgg tgcgtcctcg
     1321 tcgatgacgg ggaccggggt cgtggtcggc accggcacgg cggtcaagtc ggggttcggg
     1381 tcggccaggt gccgcccgtc gtcgggcccg caggcggcca cgaccaggac ggtgagcgcc
     1441 gcgcccgcca gccagcggag cagacgggag gggagggcca cggggggcag tattccgcct
     1501 tgccggtcgg gttcggcgga ccgaggccct tccggtgccg tcctacgctc ggggcatgac
     1561 gaccatccat cacgacgacg acggcgacct cggcgtcctg gccggccagc gtgtcgccgt
     1621 cgtcggctac gggaaccagg gccgctcgtg ggccctcaac ctccgggatt ccgggcttta
     1681 cgtccaggtc tgcgtccggg ccgacgccac ccgtgagcag gcggaggccg acgggttcac
     1741 cgcggccgac atcgccgcgg ccgatgacgc cgacgtggtc tgcgtcctgg tgcccgacga
     1801 cgccatcccg acattggctc tgacaccggg cccggcggcc ctgaccgtcg tggccagcgg
     1861 ctacacgctg gccttcgacc ggtttgcccc ggccggcgac atcggcatgg tcgccccccg
     1921 catgctcggt cccgaggtcc ggcgctgcta cgaggagggc ggcggcttca tcaccgccgt
     1981 cggcgtgcac cgggacctca cgggcacggc ccaggcccgc acgctggcgg tggccaaggg
     2041 catcggtggc ctgcgccagg gcgcgatcga gctcaccgcc cggcaggagg ccgtcctcga
     2101 cctggccgtc gagcaggtcc tctcgccggc gctgacccac gtgtccaaca acttcgtcgg
     2161 cgtgatgctg gagcagggga ttcccctgga ggccgtcatc accgaactgg tcctgtcggg
     2221 ggaggtcgag cgcacctatc gcctcctgcg ggaggagggg ttcgcccggc agatggcgta
     2281 ccactcgccg accagccagt acggccagct gtcgcgtcgg ggtcgctacg acgacctcga
     2341 tgtcgggacc acgatgcggg agctggtcga cgacatcacg tccgggcgct ttgccgacga
     2401 gtgggacgcc gagcaggaca cgggccacgc ccggctggcg gccctgaccg cgacctacgc
     2461 cggcccggag gtccaggcct tcgaggacga gatgcggacg cggctcgggg agcgggccgc
     2521 cggctgaccg cgggcggggt actccggagg aaagaccacc taccctgagg caatgacgcg
     2581 gcgcagcctc gcggagcagc tccgacgacc ggacccgatc ggcagagccg gccggacgga
     2641 gtacggcgga atgtgatgga cgacaagccc aggaacctcc gctcgatgct cacggaggcc
     2701 aaggacgcgt ccgagctgat gctcgacctg gcctacgcgt cggtctactt cggtgacccg
     2761 gacatggccg aggaggtcga cgagctcgag gaccagatga acgacctc
//
LOCUS       DASTFV010000097         2910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_332926, whole genome shotgun sequence.
ACCESSION   DASTFV010000097 DASTFV010000000
VERSION     DASTFV010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332926"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFP02_01760"
     CDS             <1..372
                     /locus_tag="VFP02_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9541777.1"
                     /translation="KRTGDSRTSVADGLAATAKVITAAAAIMVVVFGSFVLESDRTFK
                     LMGTGLATAIFLDATIVRMLLVPATMELLGDRNWWLPKWLDRILPNVDIEGHSELAVH
                     AGDDDLDEDDPRRDADLVGVD"
     gene            complement(382..1011)
                     /locus_tag="VFP02_01765"
     CDS             complement(382..1011)
                     /locus_tag="VFP02_01765"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET9541778.1"
                     /translation="MGMLRFSFGTMGSGKSTLALQIHHNLASRDLRGLLCTQLDRDGA
                     QVTSRLGVSAGAVDVGHKLDLEDLCHSFATRHGGLDYLVCDEAQFYTPAQVEQLARVV
                     DDLDVDVHALGLLTDFRGRLFPGSARFLELADERVEVQVEARCWCGARATQNARLVDG
                     DQVYDGDVVVVGDTDHEARVSYELLCRRHWMAGETGRASSIVGALSDAG"
     gene            complement(1067..2842)
                     /locus_tag="VFP02_01770"
     CDS             complement(1067..2842)
                     /locus_tag="VFP02_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET9541779.1"
                     /translation="MRRHRFALLATALAAAVLVPAAAPSAATPARRVPGIAFTSTPTS
                     GATGTVIDVAGRRCLLPGGAEPGEGVVVRLRSQGQVLAFTTLPVARNGTWSGHLPVPA
                     GTPARLHQLDARCVYPEVPDPVVYGARTFTVTGEGAGAESTPATPTFNGGIEPYGEYD
                     GQSTCSPSIKPGMAAFMRIVQRNYGGGSLGVSRSCGAGGQSEHKEGRAWDWAMNAGSA
                     GDRSRVQALMNWLFATDAQCNHYARARRLGVMYIIWNRHMFRMYDVDRGWAPYSGSSP
                     HTDHVHFSLTRDGGAGSVSFYHPTFRAPAGWTTARHRVRDVTVGPALDGLQPLTGDFD
                     GDERDDLLWYDPVAGNGQVWYTREDGRFVARDLTMGPGRAPVIGDFNGDCRDDVFWYG
                     PGAVPDRQWQGLANRTFRSVAETVGGTYQQPVAGDFNGDRVSDILWYNPGPGEDRLWR
                     GSPYGFVARSINVGGNYRPFTGDFDGDLRDDVFWYGPGSAPDLLWYGRTTGFTPHDVT
                     VAGDTTPLAGDFNGDDRTDIFWYGVGEAPDRMWTGRSNRSFTGHAVDAARPYERPAVG
                     DFDGDLQDDVLWHASPLHEDRIWHF"
BASE COUNT          419 a         1000 c         1087 g          404 t
ORIGIN      
        1 aagcggaccg gcgacagccg cacctcggtg gccgacgggc tggccgccac ggccaaggtc
       61 atcaccgccg cggcggcgat catggtcgtg gtcttcggca gcttcgtgct cgagtccgac
      121 cgcacgttca agctgatggg cacggggctg gccacggcca tcttcctcga cgccacgatc
      181 gtgcggatgc tgctggtccc ggcgacgatg gaactgctgg gtgaccggaa ctggtggttg
      241 ccgaagtggc tggaccggat cctgcccaac gtcgacatcg agggccactc cgagctcgcc
      301 gtccatgccg gcgacgacga tctcgacgag gacgacccgc ggcgcgacgc cgacctggtc
      361 ggcgtcgact gacccggcag ctcagccggc gtcgctgagg gcgcccacga tcgacgaggc
      421 ccgccctgtc tcgcctgcca tccagtggcg gcggcacagc agctcgtagc tgacccgggc
      481 ctcgtgatcg gtgtcgccca cgaccaccac gtcgccgtcg tagacctggt cgccgtcgac
      541 caggcgggcg ttctgggtgg ctcgggcgcc gcaccagcag cgggcctcga cctggacctc
      601 gacccgctcg tcggccagtt ccaggaaccg ggcggagccc gggaacaggc gaccgcggaa
      661 gtcagtgagc aggccgaggg cgtggacgtc gacgtccagg tcgtcgacca cgcgcgccag
      721 ctgctcgacc tgggccggtg tgtagaactg ggcctcgtcg cacacgaggt agtcgaggcc
      781 accgtgtcgg gtggcgaagc tgtggcagag gtcctcgaga tcgagcttgt ggccgacgtc
      841 gacggcgccg gccgacacgc cgaggcggga ggtcacctgc gccccgtccc ggtcgagctg
      901 ggtgcagagc agcccccgta ggtcccgaga ggcgaggttg tggtggatct ggagggccag
      961 agtgctcttc cccgatccca tcgtcccgaa cgagaaccgc agcatcccca tgggcgaaga
     1021 acttacaccg ctgtagttcg ccctgccgca ccgagcggcg cggcgctcag aagtgccaga
     1081 tgcggtcttc gtgcagaggg ctggcgtgcc agagcacgtc gtcctggagg tcgccgtcga
     1141 agtcacccac cgcaggtcgc tcgtagggac gggcggcatc gacggcgtgg cccgtgaagc
     1201 tccggttcga gcggcccgtc cacatgcggt ccggggcctc accgacgccg taccagaaga
     1261 tgtcggtgcg gtcgtccccg ttgaagtcgc ccgccagagg ggtggtgtca ccggcgacgg
     1321 tcacgtcgtg gggggtgaag ccggtggtcc tgccgtacca gagcaggtca ggagccgatc
     1381 ccggcccgta ccagaagacg tcgtcgcgga ggtcgccgtc gaagtcgccg gtgaacgggc
     1441 ggtagttccc acccacgttg atcgagcggg cgacgaagcc gtacggcgag ccccgccaca
     1501 gccggtcctc gccggggccg gggttgtacc agaggatgtc cgacacccgg tcgccgttga
     1561 agtcgccggc gaccggctgc tgataggtgc cccccacggt ctcggccaca gagcggaagg
     1621 tccggttggc caggccctgc cattgacggt caggcaccgc gccggggccg taccagaaga
     1681 cgtcgtcgcg gcagtcgccg ttgaagtcgc cgatgacggg ggcccggccc gggcccatgg
     1741 tcaggtcccg ggcgacgaag cgaccgtcct cgcgggtgta ccagacctgc ccgttcccgg
     1801 ccacggggtc gtaccagagc aggtcgtcgc gctcgtcccc gtcgaagtca ccggtcagcg
     1861 gctgaaggcc gtccagggcg gggcccaccg tgacgtcgcg cacgcggtgc cgggccgtgg
     1921 tccacccggc cggggcgcgg aaggtggggt ggtagaagct gacgctgccg gcaccgccgt
     1981 cgcgggtcag gctgaagtgc acgtggtcgg tgtgcgggct cgagcccgag tacggggccc
     2041 acccgcggtc gacgtcatac atccggaaca tgtgccggtt ccagatgatg tacatgacgc
     2101 cgagccggcg ggcccgggcg tagtggttgc actgggcgtc ggtggcgaag agccagttca
     2161 tcagggcctg cacccgggac cggtccccag cgctgcccgc gttcatggcc cagtcccaag
     2221 cccggccctc cttgtgctca ctctggccgc ccgcgccgca gctccggctc acgccgaggc
     2281 tgccgccgcc gtagttgcgc tggacgatcc gcatgaaggc ggccatgccc ggcttgatgc
     2341 tgggactgca ggtggactgc ccgtcgtact cgccgtaggg ctcgatgccc ccgttgaacg
     2401 tcggcgtggc gggggtcgac tcggccccgg cgccctcgcc ggtgaccgtg aaggtgcggg
     2461 cgccgtagac cacggggtcg ggcacctccg ggtagacgca ccgggcgtcg agctggtgga
     2521 gccgggccgg agtgcccgcg ggcaccggca ggtgccccga ccaggtgccg ttgcgggcga
     2581 cggggagggt ggtgaaggcc aggacctgac cctggctgcg gaggcggacg accacgccct
     2641 caccgggctc ggccccgccg gggagcagac agcgccggcc cgccacgtcg atgacggtgc
     2701 ccgtggcgcc cgacgtcggg gtgctggtga acgcgatgcc gggtacgcgc cgggccggtg
     2761 tagcggcaga cggcgccgcc gcgggcacga gcacggcggc ggccagcgcg gtggcgagga
     2821 gggcgaagcg gtgccggcgc accgtcgaac cttcggtcct gatgacagtc ccctttaccg
     2881 ccctccgcgt acgaccacga ggctaggcgc
//
LOCUS       DASTFV010000098         2526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_333470, whole genome shotgun sequence.
ACCESSION   DASTFV010000098 DASTFV010000000
VERSION     DASTFV010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333470"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1550
                     /locus_tag="VFP02_01775"
     CDS             <1..1550
                     /locus_tag="VFP02_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET9541780.1"
                     /translation="FALDFLGPEEFREGDVFVANDPYHGGGHLPDYNVFAPVYGTQAD
                     GSRRMVLICSIQCHHGDTGGSVPGGYNVTAADIWGEGARWPVVKVIDRGEERRDVLYA
                     LQANNRLPDYIGDLRAQMGAAQMAAERLGAMIDRYTLATVEESVDYMIDYASLRFREE
                     VASWPDGSYEGDAYVDHDPLGNPDIHLHVKITVDGDRLVIDYSGSDTRDEIQAWSTYG
                     NTRGYTVAQIASMMDPEIPKNEGFFDSLELVVPSGCVLNPAPGKPVSAGTHHPGADIG
                     EVIAVAMQHVLPERSVPQTYKTGIPTIIRGVDPRSGATFTDHSAEVYAGWCNAAKGMD
                     AWGCQAASFGNLWKATAEINESLFPHVQWSRDYRTDSGGPGQWRGLCGSHYRKEVRVP
                     SHVYTYVVGMKYPMPGIAGGGPGAPNAMVLRADSDDPYRVTHTADWVPMEAGDIVQYD
                     YGGGGGWGDPLDREPQAVLDDVLDEYVSVAGAEGDYGVVLTGSLEDLTLAVDEAATAA
                     LRDERRG"
     gene            1554..>2526
                     /locus_tag="VFP02_01780"
     CDS             1554..>2526
                     /locus_tag="VFP02_01780"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET9541781.1"
                     /translation="MGYRIGVDVGGTFTDLIAVTPTGEVVLEKTPSTLDDQSRGVMTG
                     LGQLAEQFGLSLTELCDDVDVLVHGTTTADNTMIEMDGAATGLLVTKGHRDEIEMRRV
                     HKEQIWDPAAPGPVPIAKRRARIPIPERVDFEGKVLLDLDEEAVRRGVQRLKALGVES
                     IAVMFLFSFVNPDNERRAAEIIREEFPDVAHVSLSHEVMPRGPEYERVSTTLVNAYVA
                     PRIAHYVDNLSATLREAGYRGPLLIMQSTGGVMPPEHVRGQAVSLLGSGPTGGVMGAA
                     VAAERGGEPDFVAIDMGGTSFDICLVRGGRPEIKTDWNWRYRYYIGLP"
BASE COUNT          401 a          930 c          850 g          345 t
ORIGIN      
        1 gcttcgccct cgacttcctc gggcccgagg agttccgcga gggcgatgtc ttcgtcgcca
       61 acgacccgta ccacgggggc ggccacctcc ccgactacaa cgtcttcgcc ccggtgtacg
      121 gcacccaggc cgacggcagc cggcggatgg tcctgatctg ctccatccag tgccaccacg
      181 gcgacaccgg gggctcggtg cccggtggct acaacgtcac cgcggccgac atctggggcg
      241 aaggggcccg ttggccggtg gtcaaggtca tcgaccgggg cgaggagcgc cgcgacgtcc
      301 tctacgccct ccaggccaac aaccgcctgc ccgactacat cggcgacctg cgggcccaga
      361 tgggggcggc ccagatggcg gccgagcgcc tcggggccat gatcgaccgc tacacgctgg
      421 ccaccgtcga ggagtcggtc gactacatga tcgactacgc ctccctccgc ttccgggagg
      481 aggtcgcctc ctggcccgac ggttcctacg agggcgacgc ctacgtcgac cacgacccgc
      541 tgggcaaccc cgacatccac ctccacgtga agatcacggt ggacggcgac cgcctggtca
      601 tcgactactc gggcagcgac acgcgcgacg agatccaagc ctggtcgacc tacgggaaca
      661 cccggggcta cacagtggct cagatcgcct cgatgatgga cccggagatc cccaagaacg
      721 agggcttctt cgactccctc gagctggtgg tccccagcgg ctgcgtcctg aacccggcgc
      781 cgggcaagcc ggtgtcggcc ggcacccatc accctggcgc cgacatcggc gaggtcatcg
      841 ccgtggccat gcagcacgtg ctcccggagc ggtccgtccc ccagacctac aagacgggca
      901 tccccacgat catccggggc gtcgaccccc gcagcggggc caccttcacc gaccactcgg
      961 ccgaggtcta cgccggctgg tgcaatgcgg cgaagggcat ggacgcctgg ggctgccagg
     1021 ctgcctcctt cggcaacctc tggaaggcca ccgccgagat caacgagagc ctgttccccc
     1081 acgtccagtg gagccgggac taccgcaccg actcgggcgg tcccggccag tggcggggcc
     1141 tgtgcggaag ccactaccgc aaggaggtcc gggtccccag ccacgtctac acctacgtcg
     1201 tcggcatgaa gtacccgatg cccggcatcg ccggcggcgg ccccggcgcg cccaacgcca
     1261 tggtcctccg ggccgactcc gacgacccct accgcgtcac ccacaccgcc gactgggtgc
     1321 ccatggaggc cggcgacatc gtccagtacg actacggcgg cggcggcggt tggggtgatc
     1381 ccctggaccg agagccccag gccgtgctcg acgacgtgct cgacgagtac gtcagcgtgg
     1441 ccggagccga gggcgactac ggcgtcgtgc tcaccgggtc cctcgaagac ttgaccctcg
     1501 cggtggacga ggcggccacg gccgcgctcc gggacgagcg gcgcggctga gccttggggt
     1561 accggatcgg cgtcgatgtc gggggcacgt tcaccgacct gatcgcggtc acgcccacgg
     1621 gcgaggtcgt gctggagaag accccctcga ccctcgacga ccagtcgcgg ggcgtgatga
     1681 ccggcctcgg ccagctggcc gagcagttcg ggctctcgct gactgagctg tgcgacgacg
     1741 tcgacgtcct cgtccacggc accacgaccg ccgacaacac gatgatcgag atggacgggg
     1801 ccgccaccgg cctgctcgtc accaaaggac atcgcgacga gatcgagatg cggcgggtcc
     1861 acaaggagca gatctgggac ccggctgccc cgggccccgt ccccatcgcc aagcgccggg
     1921 cccgcatccc gatccccgag cgggtcgact tcgaaggcaa ggtcctgctc gacctcgacg
     1981 aggaggcggt gcggcggggc gtgcaacgcc tgaaggcgct gggggtcgag tcgatcgcgg
     2041 tgatgttcct gttcagcttc gtgaacccgg acaacgagcg tcgggccgcc gagatcatcc
     2101 gcgaggagtt ccccgacgtc gcccacgtct ccctgtccca cgaggtcatg ccccggggcc
     2161 ccgagtacga gcgggtctcg accaccttgg tgaacgccta cgtggccccc cgcatcgccc
     2221 actacgtcga caacctgtcg gccacgctgc gggaggccgg ttaccggggg ccgctgctga
     2281 tcatgcagtc caccggcggg gtcatgcccc ccgagcacgt ccgcggccag gccgtgagcc
     2341 tgctcggctc cggcccgacg ggtggggtca tgggcgcggc ggtggccgca gagcggggcg
     2401 gcgagcccga cttcgtcgcc atcgacatgg gcggcaccag cttcgacatc tgcctcgtgc
     2461 ggggcggccg cccggagatc aagaccgact ggaactggcg ctaccgctac tacatcggcc
     2521 tgccga
//
LOCUS       DASTFV010000099         4445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_336172, whole genome shotgun sequence.
ACCESSION   DASTFV010000099 DASTFV010000000
VERSION     DASTFV010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336172"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..654)
                     /locus_tag="VFP02_01785"
     CDS             complement(43..654)
                     /locus_tag="VFP02_01785"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET9541782.1"
                     /translation="MPRLSRENIELAREIISRYPRPRSAVIPLLHLAQEQDGYVTDEA
                     MEHIGELVGITPAEVLGTCSFYEMLKLEPVGRYLVNVCTDISCMLLGGAELLEHAEEA
                     LDLKAGNTTADGLFTLEDVECQAACTEAPCLQVNYRYFTNVAPAALDQLLEDLRAGQM
                     DGDIPPHGTLHRIRQHIPPERAAGPALPDGGQAPAWMAPAEGA"
     gene            complement(692..2020)
                     /locus_tag="VFP02_01790"
     CDS             complement(692..2020)
                     /locus_tag="VFP02_01790"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HET9541783.1"
                     /translation="MSDTEAEFADLIAQTSEESQGLSRGSGADRLREMGAVLRLSEAD
                     AAALEGEAGDPDQTMIINMGPQHPSTHGVLRLMLELQGETVLRTKPIIGYLHTGMEKT
                     GETLTYLQGATNVTRMDYASPLFNELAFALAVEQLLEVEVPVRATWIRMLMTEMNRIA
                     SHLLFLATNGMDLGAVSMMLFGWREREEVLRFFESVTGLRMNHNYIRPGGVAADLPDG
                     WEEDVLALMDLIPPRLEEYDTLMTGQPIWRERLQGVGVITADEAIALGATGPILRSTG
                     YAWDLRRDMPYLAYDELDFDVVVGSYGDAFDRYAIRLNEVRESMRLVRQIVERMPSGD
                     YRVQDKKVTPPPRARIDESMEALIHHFKLFTEGFQVPPGETYAAVESPRGELGCYIVS
                     DGGAKPYRLRVRGPSFVNLQTLPHMMRGGLMADAVAVISSVDPIMGEVDR"
     gene            complement(2017..2562)
                     /locus_tag="VFP02_01795"
     CDS             complement(2017..2562)
                     /locus_tag="VFP02_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET9541784.1"
                     /translation="MSDETGVQDAETEDAAPEPEPVAEKHGVPVTDSLGQEVLHPSRE
                     QYRDLVETLRAEGYLVCIDLTAVDYLAHPHRSVAPGVVPERFELVVSFLSHEHKGRTR
                     LRVQVPADDPTVPCIVDLHPGADALEREVFDMFGIVFEGHPDLTRILMPEDWDGHPLR
                     KDYGVGAIPVQFKGAGSEERA"
     gene            complement(2569..3174)
                     /locus_tag="VFP02_01800"
     CDS             complement(2569..3174)
                     /locus_tag="VFP02_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HET9541785.1"
                     /translation="MPERKEDGLLDKGLEGLSHNFVTGTLEELVKWARARSVMPATFG
                     LACCAIEMMATGGAHYDLARYGMETFRASPRQADLMIVAGRVSQKMAPVLRQIHDQMM
                     EPKWVISMGVCASSGGMFNNYAIVQGVDQVVPVDVYAPGCPPGPQTLMHAILTLHAKI
                     RSGELTQRREVRGGGAEIQISQVDGQTDTGAVRLLKPTTKD"
     gene            complement(3174..3641)
                     /gene="ndhC"
                     /locus_tag="VFP02_01805"
     CDS             complement(3174..3641)
                     /gene="ndhC"
                     /locus_tag="VFP02_01805"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET9541786.1"
                     /translation="MGQYLPVLALGLLAFVFAILSILASRLLAPRLMTPAKEAPYECG
                     IVPSREPPQRFPVKFFLVAMIFIVFDIEIIFFIPWAVVYRDLGAFGFWSMLIFAFLVF
                     ESFLYLVSKGALDWGPVKRVVEALGQVGAHRTSDSTIRRVGLEGREPTEEVAA"
     gene            complement(3645..>4445)
                     /locus_tag="VFP02_01810"
     CDS             complement(3645..>4445)
                     /locus_tag="VFP02_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541787.1"
                     /translation="GARLGFQVAMAGLFGWLMLLSLFWWIYGVGAIGTLPSWRVEEIN
                     VGDLSQAQLEEARGLVPENLPSAEDIIAEFPEVAEAMAEDPTPTLSEIAALPDLPPEV
                     EEQLTGLEGGWDVLSQAAIGDAQAAADTALISEDTGLYTSASEYVLVDGFTVGGKEQP
                     ESDSLWDRVSTRVANAFTILNPPKYTVIQVQTSESTAVPEGNAPLSPTPDEDQPVVSV
                     IMVRDLGTRRLPPAMLTILFGSLFALTCALLHSRDRRAQALSTVTAGD"
BASE COUNT          659 a         1463 c         1641 g          682 t
ORIGIN      
        1 cgccggctga cgatgcggtc ggtgctggcc agaggcgcca tgtcaggccc cctcggccgg
       61 ggccatccac gccggcgcct ggcccccgtc ggggagggcg gggccggcgg cgcgctcggg
      121 cgggatgtgc tggcggatgc ggtgcagggt cccgtggggc gggatgtccc cgtccatctg
      181 gccggcccga aggtcctcca ggagctggtc cagggcggcg ggcgcgacgt tggtgaagta
      241 ccggtagttg acctgcaggc agggggcctc ggtgcaggcc gcctggcact cgacgtcctc
      301 gagggtgaac aggccgtcgg cggtggtgtt gcccgccttc aggtccagag cctcctcggc
      361 gtgctccagc agctcggcgc cgccgaggag catgcaggag atgtccgtac agacgttgac
      421 caggtagcgg ccgacgggct cgagcttgag catctcgtag aaggagcagg tgccgaggac
      481 ctcggccggg gtgatcccga ccagctcgcc gatgtgctcc atggcctcgt cggtgacgta
      541 gccgtcctgc tcctgggcca ggtggagcag ggggatgacg gctgagcgcg ggcgcgggta
      601 gcgcgagatg atctcgcggg cgagctcgat gttctcgcgg gagaggcggg gcactatgcc
      661 ttgccctccg ggcgagccgt gtccacggag gtcatcgatc gacctcgccc atgatcggat
      721 ccacgctgga gatgacggcc acggcgtcgg ccatgaggcc gccccgcatc atgtgcggga
      781 gggtctgcag gttcacgaag ctcgggccgc ggacgcgcag tcggtagggc ttggctccgc
      841 catcggacac gatgtagcag cccagctccc cccggggcga ttccacggcg gcgtaggtct
      901 cccccggcgg cacctggaag ccctcggtga acagcttgaa gtggtggatc aaggcctcca
      961 tcgactcgtc gatgcgggcc cggggcggtg gggtgacctt cttgtcctgc acccggtagt
     1021 cccccgaggg catccgctcg acgatctggc gcaccagcct catcgactcc cgcacctcgt
     1081 tcaggcggat ggcgtagcgg tcgaaggcgt cgccgtagct acccaccacg acgtcgaagt
     1141 ccagttcgtc gtaggccagg tagggcatgt cccgacgcag gtcccaggcg tagccggtgg
     1201 agcggaggat gggcccggtg gccccgaggg cgatggcctc gtcggcggtg atgaccccca
     1261 ccccctggag ccgctctcgc cagatcggct ggcccgtcat cagggtgtcg tactcctcca
     1321 gccgcggcgg gatcaggtcc atcagggcga ggacgtcctc ctcccagccg tcgggcaggt
     1381 cggcggcgac gccaccgggt cggatgtagt tgtggttcat ccgcaggccg gtgaccgact
     1441 cgaagaagcg caggacctcc tcgcgctcgc gccagccgaa cagcatcatc gagacggcgc
     1501 cgaggtccat gccgttggtg gcgaggaaga gcaggtggct ggcgatgcgg ttcatctcgg
     1561 tcatcagcat gcggatccac gtggcccgga ccgggacctc gacctcgagg agctgctcca
     1621 cggccagggc gaaggccaac tcgttgaaca acggcgaggc gtagtccatg cgggtgacgt
     1681 tggtggcccc ctgcaggtag gtcagggtct cgcccgtctt ctccatgccc gtgtgcaggt
     1741 agccgatgat cggcttggtc cgtaggacgg tctcaccctg gagctcgagc atgagccgca
     1801 gcaccccgtg ggtcgacggg tgctggggac ccatgttgat gatcatcgtc tggtcggggt
     1861 cgccggcttc cccctcgagg gcggcggcgt cggcctcgga caggcggagc acggcgccca
     1921 tctcccgcag gcggtcggcc cccgaacctc gggacaggcc ctggctctcc tccgaggtct
     1981 gggcgatgag atcggcgaac tcggcctcgg tgtcgctcat gcgcgctcct cagaaccggc
     2041 acccttgaac tgcaccggga tggccccgac gccgtagtcc ttgcgcaggg ggtggccgtc
     2101 ccagtcctcg ggcatcagga tccgggtcag gtcgggatgg ccctcgaaca cgatgccgaa
     2161 catgtcgaac acctcacgct ccagggcgtc ggccccgggg tgcaggtcga cgatgcaggg
     2221 cacggtcggg tcgtcggccg ggacctggac gcgcaggcgg gtccggccct tgtgctcgtg
     2281 gctcaggaat gacaccacca gctcgaagcg ctcagggacg acaccggggg cgacggatcg
     2341 gtgcggatgg gccaggtagt ccaccgcggt gaggtcgatg cagaccagat agccctcggc
     2401 ccggagggtc tcgaccaggt cccggtactg ctcgcgagag gggtgcagca cctcctggcc
     2461 cagcgagtcg gtgaccggga cgccgtgctt ctcggccacc ggctccggct ccggggccgc
     2521 gtcctcggtc tcggcgtcct gaacgccggt ctcgtcgctc accgacggct agtccttcgt
     2581 ggtgggcttg aggaggcgga cggcgccggt gtcggtctgg ccgtcgacct ggctgatctg
     2641 gatctccgcg ccgccgccgc gcacttcccg gcgctgggtc agctcgcccg agcggatctt
     2701 ggcgtggagg gtgaggatgg cgtgcatgag ggtctggggc ccgggggggc agccgggggc
     2761 gtagacgtcg acgggcacga cctggtcgac gccctggacg atggcgtagt tgttgaacat
     2821 gccgccgctg gaggcgcaga cgcccatgga gatgacccac ttgggctcca tcatctggtc
     2881 gtggatctgg cgcaggaccg gagccatctt ctgggagacc cgcccggcca cgatcatgag
     2941 gtcggcctgg cgcggggagg cccggaaggt ctccatgccg taacgggcca ggtcgtaatg
     3001 ggcgccgccg gtggccatca tctcgatggc acagcaggcc agcccgaagg tggcgggcat
     3061 cacgctgcgg gcccgggccc acttgaccag ctcctcgagg gtgccggtca cgaagttgtg
     3121 gctgagcccc tcgaggccct tgtccagcaa gccgtcttcc ttgcgctcgg gcatcaggcc
     3181 gccacctcct cggtgggctc gcggccctcg aggccgaccc ggcggatggt cgagtcgctg
     3241 gtgcggtggg ccccgacctg accgagggcc tcgacgaccc gcttgaccgg gccccagtcc
     3301 agcgcgccct tgctgaccag gtagaggaag gactcgaaga ccaggaaggc gaagatcagc
     3361 atcgaccaga acccgaaggc cccgaggtcc cggtacacga cggcccaggg gatgaagaag
     3421 atgatctcga tgtcgaacac gatgaagatc atcgccacca ggaagaactt gaccgggaag
     3481 cgctgggggg gctcgcggct ggggacgatc ccgcactcgt agggggcctc cttggccggc
     3541 gtcatcagcc ggggcgcgag gagccgggag gccaggatgc tgaggatcgc gaagacgaag
     3601 gccagcagtc cgagggcgag caccggcagg tactggccca tcgctcagtc ccccgcggtg
     3661 acggtcgaga gggcctgcgc ccggcggtcg cggctgtgga gcagggcaca ggtgagggcg
     3721 aacaggctgc cgaacaagat cgtcagcatc gcaggcggca gccgccgcgt cccgaggtcc
     3781 cgcaccatga tcaccgacac gacgggctgg tcctcgtccg gggtgggcga caggggggcg
     3841 ttgccctccg gcactgcggt ggactccgag gtctggacct ggatcaccgt gtacttcggt
     3901 gggttcagga tcgtgaaggc gttggcgacc cgggtcgaca cccggtccca gaggctgtcg
     3961 ctctccggct gctccttgcc cccgacggtg aacccgtcca ccagcacgta ctcgctggcc
     4021 gaggtgtaga gcccggtgtc ctcgctgatc agggcggtgt cagccgcggc ctgggcgtca
     4081 ccgatcgcgg cctgggacag cacgtcccag cccccctcga gaccggtcag ctgctcttcg
     4141 acctcggggg gcaggtcggg cagggcggcg atctcgctca gggtgggggt ggggtcttcg
     4201 gccatcgcct cggccacctc ggggaactcg gcgatgatgt cttcggccga gggcaggttc
     4261 tcgggcacga ggccccgggc ctcctcgagc tgggcctggc tcaggtcacc gacgttgatc
     4321 tcctcgaccc tccacgaggg cagggtcccg atggctccca cgccgtagat ccaccagaag
     4381 aggctgagca gcatcagcca gccgaagagg ccggccatgg ccacctggaa gccgaggcgg
     4441 gcccc
//
LOCUS       DASTFV010000100         4825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_336656, whole genome shotgun sequence.
ACCESSION   DASTFV010000100 DASTFV010000000
VERSION     DASTFV010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336656"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3067)
                     /locus_tag="VFP02_01815"
     CDS             complement(<1..3067)
                     /locus_tag="VFP02_01815"
                     /inference="COORDINATES: protein motif:HMM:NF023275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-(1->3)-arabinofuranosyltransferase family
                     protein"
                     /protein_id="HET9541788.1"
                     /translation="MPPRTSSASSRLRRSLPLLLLAAVTYLPLVFTQPGQVGADTKTY
                     LYVDPGRLLRDAAFLWDPGVGLGGVTHQVIGYLWPMGPFYWFLDAIGVPDWLAQRLWL
                     GSLMFAAGAGVLYLLRTLGWPDRPAGHDDEKPSSATWWDAGMVVAALAYALSPYVLDY
                     AARISVILLPWAGLPWLIAFLARAIRVGGWRYPAAFALVTLTISSTNLTSVVLVGIGP
                     ILWVAFAVWIEREVPLRQALAAVWRAGLLTVGVSLWWFVGLVVEGRYGIPIVRYTETY
                     QAVAVASTTPEVIRGLGYWFFYGNDKLGQWIIPSIHYMRYGIPLSFALPIFAFLSGAL
                     TRFRHRLFFITLIVIGAVMAVGGHPYDDPSPAGGLLVAWTRTDIGLAFRSTARVLPLL
                     VLGTSVLLGTGVVALGRRLPRLATPIGVLAVILVIANLPAPWVGQMVDRNLRRDEDLP
                     DYWVDAAAHVDGRDDGTRVLELPGSDFAAYRWGNTVDPITPGLMDRPYVARELIPLGT
                     PVSANLVNAIDRPLQEGTFDPDALAPLARLIGVGDIVLRSDLEYERFRTPRPETTWAL
                     LQSVPGLGEPVAFGEPVENQPREQLPLLDEIELATDQTLDDPPPVAVFPVDDAAPIVR
                     ALPAEHPIVVSGDGDGLVAATSAGVIDPDRAILYSAGLLDEDDPAMFDIVLDQGADIV
                     ITDSNRRRARRWGAIRENEGYTEMAGEESLEYDVGDARLDSFPTASDDSFTVAVQRGG
                     ATIQASAYGNPVSYTPGDRAFFAIDGDPRTAWKVGAFSEVEGEYLRVTTDEPVTTDHI
                     GLTQPLSTDINRWITEVELRFDGGDPVRVDLTEDSRTRPGQVIEFPERTFQRLDIEIL
                     GDTIGFRPKLAGVSAVGFSEVDLDGLQLEELIRPPVDLVDAVDGDLTDHRLSYVLQRQ
                     RTNPAEPVRVDEEPAMRRLLDVPGERAFELDGEARISAQAPDPLVDRLLGLPGAGAGG
                     LTARSSAHLAGSLAARASSAVDGDPTTAWTTPFNALMGQYMEVELPEAHT"
     gene            3142..3873
                     /locus_tag="VFP02_01820"
     CDS             3142..3873
                     /locus_tag="VFP02_01820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9541789.1"
                     /translation="MSASDEAQLEMVHRLPKAQLVQRIPYVAEQVRGRGVIHVGFVDA
                     GYQEMQAEAGTWLHAHLAEAATSLVGIDLDAAGVALATDQGYEAYVADCRDPEQIDAL
                     AIEPAEVVVAGEVIEHLDDPGSFLDGLHRLLAPGGTLILTTPNASGLLNGFAAMAGAE
                     INHPDHVVLFSWRTLSNLMGRHGWEVVATATFVPAVKAAPGSGLRQRVLGLAGRFVLW
                     LERTIGRLWAPFVADGLIVTAREKP"
     gene            complement(3879..4274)
                     /locus_tag="VFP02_01825"
     CDS             complement(3879..4274)
                     /locus_tag="VFP02_01825"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HET9541790.1"
                     /translation="MSPRAQLLVMAIVAFSLLFILRLVRRRKLQGKYALLWVSVGLFL
                     GAFALIPDVLVPIADWVGIAYEPAVFFLAAIAFLFLMVVHFSFELSRSEERTRTLAEE
                     VALLKARMDKTDPPFDRTPTAPVDDAPED"
     gene            complement(4271..>4825)
                     /locus_tag="VFP02_01830"
     CDS             complement(4271..>4825)
                     /locus_tag="VFP02_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9541791.1"
                     /translation="LSLPFNLGIGGALRTGFLYAVRNGYERAVQFDGDGQHDADEIPT
                     LLAALADGADMVVGSRFAGKATSYSVSRVRARAMGVLRFTIKTLSGQQFTDTSSGFRA
                     FNREVLEFFARTYPSEYMESVEALLLACAEGFRVAEVPVNMSDREAGTPSNRRWRLIY
                     HYLRLLVVLVASAPRRAVVREAKA"
BASE COUNT          753 a         1605 c         1836 g          631 t
ORIGIN      
        1 ccgtgtgcgc ctcgggcagc tcgacctcca tgtactggcc catcagggcg ttgaagggcg
       61 tcgtccaggc ggtggtcggg tcgccgtcca ctgccgagga cgcccgggcc gccagcgacc
      121 cggccaggtg ggccgaggag cgggcggtga ggcctcccgc gcccgcgccg ggcaggccca
      181 ggaggcggtc gaccagggga tccggggcct gggccgagat gcgggcctcg ccgtccagct
      241 cgaacgcccg ctcaccgggg acatcgagca ggcgccgcat ggccggctcc tcgtcgaccc
      301 ggaccggctc ggccgggttg gtccgttggc gttgcagcac gtagctcagg cggtggtcgg
      361 tcaggtcgcc gtcgacggca tcgacgaggt cgaccggcgg acggatgagc tcttccaact
      421 gcagcccgtc gagatcgacc tcggagaagc ccacggcgct gaccccggcc aacttgggcc
      481 ggaagccgat ggtgtcgccc aggatctcga tgtcgaggcg ctggaaggtc cgctcgggga
      541 actcgatgac ctggccgggg cgggtccggg agtcctcggt caggtccacg cgcaccgggt
      601 cgccaccgtc gaagcgcagc tcgacctcgg tgatccagcg gttgatgtcg gtcgagaggg
      661 gctgggtcag gccgatgtgg tcggtggtca ccggctcgtc ggtcgtgacc cgcaggtact
      721 caccctcgac ctcgctgaag gcgccgacct tccaggcggt tcgggggtcc ccgtcgatgg
      781 cgaagaacgc ccggtcgccc ggggtgtagc tgaccgggtt gccgtaggcg ctggcctgga
      841 tcgtggcccc gccgcgctgc acggcgaccg tgaagctgtc atcggacgcc gtggggaagc
      901 tgtcgagacg ggcatcgccg acgtcgtact cgagggactc ctcgccggcc atctccgtgt
      961 agccctcgtt ctcccggatg gccccccagc gccgggcccg acgccggttg gagtcggtga
     1021 tgacgatgtc ggcgccctga tccaggacga tgtcgaacat ggccgggtcg tcctcgtcga
     1081 ggagtccggc gctgtacagg atcgcccggt cggggtcgat cacgcccgcc gaggtggccg
     1141 ccaccaggcc gtcaccgtcg ccggagacca cgatggggtg ctcggcgggc agggcccgca
     1201 cgatcggagc ggcgtcgtcg accgggaaga cggccacggg gggcgggtcg tcgagggtct
     1261 gatcggtggc cagctcgatc tcgtccagca ggggcaactg ctcgcggggc tggttctcga
     1321 ccggttcgcc gaaggccacc ggctcaccca ggccgggcac ggactggagc agcgcccacg
     1381 tggtctccgg gcgaggcgtg cggaagcgct cgtactcgag gtcggagcgc aggacgatgt
     1441 cgccgacacc gatgaggcgg gcgagggggg ccagtgcgtc ggggtcgaac gtgccctcct
     1501 gcagcggccg atcgatggcg ttgaccaggt tggccgagac cggggtgccc agggggatca
     1561 gctcccgggc cacgtacggg cggtccatca ggcccggcgt gatcgggtcg acggtgttgc
     1621 cccagcggta ggcggcgaag tccgagcccg gcagctcgag gacccgggtg ccgtcgtccc
     1681 ggccgtcgac gtgggcggcg gcgtcgaccc agtagtcggg caggtcctcg tcgcggcgca
     1741 ggttccggtc caccatctga ccgacccagg gcgcgggcag gttggcgatg accaggatca
     1801 ccgccagcac cccgatcggc gtggccaagc gagggagtcg acgccccagg gcgacgacgc
     1861 ccgtgccgag gaggaccgag gtccccagca ccagcaacgg cagcacgcgg gccgtgctcc
     1921 ggaaggcgag cccgatgtcg gtcctggtcc aggccaccag caggccaccc gcgggtgacg
     1981 gatcgtcgta gggatgaccg ccgaccgcca tgacggcccc gatcacgatc agggtgatga
     2041 agaagagccg gtgtcggaag cgggtcaggg cgccggacag gaaggcgaag atcggcaggg
     2101 cgaacgacag ggggatgccg tagcgcatgt agtggatgct ggggatgatc cactggccga
     2161 gcttgtcgtt gccgtagaag aaccagtagc cgaggcctcg gatgacctcc ggtgtcgtgg
     2221 aggccaccgc cacggcctgg taggtctcgg tgtagcggac gatggggatg ccgtagcggc
     2281 cttccacgac caggccgacg aaccaccaga gggacacgcc gacggtcagc agcccggcgc
     2341 gccacacggc ggccagggcc tgccggagcg gcacctcccg ctcgatccag acggcgaagg
     2401 cgacccagag gatcgggccg atgccgacga ggacgaccga ggtgagattg gtgctgctga
     2461 tggtcagcgt caccagggcg aacgcggccg gatagcgcca accgccgacc cggatggccc
     2521 gggcgaggaa ggcgatgagc cagggcaggc cggcccacgg cagcaggatg accgagatgc
     2581 gggccgcgta gtccaggacg taggggctca gggcgtaggc cagggcggcc acgaccatgc
     2641 ccgcgtccca ccaggtcgcc gaggagggct tctcgtcgtc gtggcccgcc ggtcggtcgg
     2701 gccaaccgag ggtgcgcagg aggtagagga cgccggcgcc ggcggcgaac atgaggctgc
     2761 ccagccagag gcgctgggcc agccagtcgg gcacgccgat ggcgtcgagg aaccagtaga
     2821 aggggcccat cggccacagg tagccgatga cctggtgggt caccccgccc aggcccacac
     2881 cggggtccca gaggaaggcg gcgtcccgca ggaggcggcc cgggtccacg tacaggtagg
     2941 tcttcgtgtc cgcgccgacc tggcccggct gggtgaagac caggggcagg taggtgaccg
     3001 ccgccaggag cagcagcggg aggctgcggc gtaggcgcga cgaggcggag gaggtacggg
     3061 gaggcatcag gacgagcggt ccgtcacgct accggggcgg gcccgacccc tgggggcggg
     3121 cgaccggtag cgtgcgacgc cgtgtccgcg tccgacgagg ctcagctcga gatggtgcac
     3181 cggctgccca aggcgcagct ggtgcagcgc atcccctacg tggccgaaca ggtccggggg
     3241 cgcggggtga tccacgtcgg gttcgtcgac gccggctacc aggagatgca ggccgaggcc
     3301 ggcacctggc tgcacgccca cctggcggag gcggcgacga gcctggtcgg catcgacctg
     3361 gacgcggccg gcgtggccct cgccacggac cagggttatg aggcctacgt ggccgactgc
     3421 cgggacccgg agcagatcga tgccctcgcc atcgagcccg cggaggtggt ggtggccggc
     3481 gaggtcatcg agcacctcga tgaccccggt tccttcctcg acggcctgca ccgcctgctg
     3541 gcccccggtg gcaccctcat cctgaccacc cccaacgcct cgggcctgct caacggcttc
     3601 gccgcgatgg ctggggccga gatcaaccat cctgaccacg tcgtgctctt cagttggcgc
     3661 accctgtcca acctcatggg ccgccacggc tgggaggtgg tggccacggc cacgttcgtg
     3721 cccgccgtga aagccgctcc cgggtcgggc ctgcgccagc gggtcctggg cctcgccggc
     3781 cgcttcgtcc tctggctgga gcgcaccatc ggtcgcctct gggctccctt cgtcgccgac
     3841 ggcctgatcg tcaccgcccg cgagaagccg tagcgacctc agtcctccgg cgcgtcgtcc
     3901 acgggcgcag tcggcgtccg gtcgaacggg gggtcggtct tgtccatccg ggccttgagc
     3961 agggcgacct cctcggcaag cgtccgcgtg cgttcctcgg agcgcgacag ctcgaaggag
     4021 aagtggacga ccatgaggaa caagaacgcg atggcggcga ggaagaagac ggccggctcg
     4081 taggcgatgc ccacccagtc ggcgatgggc accaggacgt cggggatcag ggcgaaggcg
     4141 ccgaggaaca gcccgacact gacccagagc agggcgtact tgccctgcag cttgcgccgg
     4201 cggaccaggc gcaggatgaa gagcagcgag aaggccacga tggccatgac caggagttgg
     4261 gcccggggac tcatgccttc gcctcccgga ccacggctcg acggggggca gacgcgacca
     4321 ggacgacgag gagccgcagg tagtggtaga tgaggcgcca acgccggttc gacggggtac
     4381 ccgcctccct gtcgctcatg ttcaccggca cctcggccac ccggaacccc tcggcacagg
     4441 ccaggagcag ggcttcgacg gactccatgt actccgacgg ataggtgcgg gcgaagaact
     4501 cgagcacctc gcggttgaag gcccggaagc cggagctggt gtcggtgaac tgctggccgg
     4561 acagggtctt tatggtgaat cggagcacgc ccatggcccg cgcccgcact cggctcacgc
     4621 tgtaggaggt ggccttgccg gcaaagcggc tgcccacgac catgtcggca ccgtcggcca
     4681 gcgccgccag cagggtgggg atctcgtcgg cgtcgtgctg gccgtcgccg tcgaactgca
     4741 cggcccgctc gtagccgttg cggacggcgt agaggaagcc cgtgcgcagc gcgccgccga
     4801 tgccgaggtt gaaggggagc gagag
//
LOCUS       DASTFV010000101         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_339375, whole genome shotgun sequence.
ACCESSION   DASTFV010000101 DASTFV010000000
VERSION     DASTFV010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339375"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VFP02_01835"
     CDS             complement(<1..823)
                     /locus_tag="VFP02_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9541792.1"
                     /translation="MGHPREEVEEAFAEFIRRGVENRDWPAWAALFTDDAQYIEHNLG
                     VFHGRPAIHDWIVDAMAPFPNMTFSVDWHVIEGDRVAFYIWNHLPDPAGGDAFYSFPN
                     ISLIDYAGDGTWSREEDFYNPADANRAVGNWYRAGGSKETSENDDLVAPEDWAPLPRP
                     PEHERSEVEAAFHRYVERGRQAVATGDWETWSKQFVEDARYFEHHYGRFNGRAEIRDW
                     IVGVMQPFPEMDFPTHWYGIDGNRVVFIAGNRLPVPTGGDTTYEVPACVVLHYAGD"
     gene            879..1664
                     /locus_tag="VFP02_01840"
     CDS             879..1664
                     /locus_tag="VFP02_01840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9541793.1"
                     /translation="MQLVGKVVVITGGGNGIGAAMARRFTEEGPAAVVVADIDGDAAA
                     AVADEIDGRSVTVDVADEAQVQDLVASTLRDHGRIDLFCANAGVGAFGGVEVPDEAWQ
                     RSWDVNVMAHVYAARALLPDWLERDEGYLLHTASAAGLLTNIGAAPYSVSKHAVVGLA
                     EWLSMTHGGGGVRFSCLCPMGVRTNMVAGGKDVAAGAVVINQGLIEPEDCAEAAVDGL
                     ATERFLILPHPEVAEFVLRKATDHDRWLAGMRRLQATIEGGLS"
     gene            1661..>2073
                     /locus_tag="VFP02_01845"
     CDS             1661..>2073
                     /locus_tag="VFP02_01845"
                     /inference="COORDINATES: protein motif:HMM:NF021489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET9541794.1"
                     /translation="MRQAKKLLGVLFPLLLIGGFAIIGAVSGDVDDGSGSDKSFDLES
                     IDVNTALRRDGVLLVSEEVTYDFDGTFNVGTRDFDAGPWAITNIRAYEDDQPLDTITN
                     TPTLFEWDLSAASGTHTYELRYEVRDAVIAWPDVV"
BASE COUNT          298 a          706 c          736 g          333 t
ORIGIN      
        1 cgtcgccggc gtagtggagg acgacgcagg cggggacctc gtacgtcgtg tcgccgccgg
       61 tggggacggg caggcggttg ccggcgatga agacgacccg gttgccgtcg atgccgtacc
      121 agtgggtcgg gaagtccatc tcggggaagg gctgcatgac cccgacgatc cagtccctga
      181 tctccgcccg gccgttgaag cggccgtagt ggtgttcgaa gtagcgagcg tcctcgacga
      241 actgcttgga ccaggtctcc cagtcgccgg tggccacggc ctgacggccc cgctcgacgt
      301 aacggtggaa ggcagcctcc acctcgctgc gctcgtgctc gggtgggcgg ggcaggggcg
      361 cccagtcctc gggagcgacg aggtcgtcgt tctccgacgt ctccttggag cccccggccc
      421 ggtaccagtt gccgaccgcc cggttggcgt cggccgggtt gtagaagtcc tcttcgcggg
      481 accacgtccc gtcgccggca tagtcgatga ggctgatgtt ggggaaggag tagaaggcgt
      541 cgccgccggc cgggtcaggc aggtggttcc agatgtagaa cgccacccgg tcgccttcga
      601 tcacgtgcca gtccaccgag aaggtcatgt tcgggaacgg cgccatggcg tccacgatcc
      661 agtcatggat cgcgggccgg ccgtggaaga cccccaggtt gtgctcgatg tactgggcgt
      721 cgtcggtgaa cagcgcggcc caggccggcc agtcccggtt ctccacccct cgacggatga
      781 actcggcgaa cgcttcctcg acttcttcac gcgggtgccc catgggcggg gaaggtagtg
      841 cagcccgtgc gctcgccccg gccgtacgct ctccgcccgt gcaactggtc gggaaggtcg
      901 tggtcatcac gggcggtggc aacggcatcg gggcggcgat ggcccgacgt ttcaccgagg
      961 agggtccggc cgccgtggtg gtggcggaca tcgacggtga cgcggccgcc gcggtggccg
     1021 acgagatcga cggccggtcc gtcaccgtcg acgtggccga cgaggcccag gtgcaggacc
     1081 tggtcgcgtc gacgctgcgg gaccacggcc ggatcgacct gttctgtgcc aatgccggcg
     1141 tgggcgcctt cggcggggtc gaggtccccg acgaggcctg gcagcgctcc tgggacgtca
     1201 acgtcatggc ccacgtctac gcggcccggg cgctgctccc ggactggctg gagcgggacg
     1261 agggctacct cctccacacc gcgtcggccg ccggcctcct gaccaacatc ggcgctgcgc
     1321 cctactcggt gtccaagcac gccgtggtgg gactggccga atggctgtcc atgacccacg
     1381 gcggcggtgg ggtgcgcttc tcctgcctct gccccatggg cgtgcgcacc aacatggtcg
     1441 ccgggggcaa ggacgtcgcg gccggagcgg tggtcatcaa ccagggcctg atcgagcccg
     1501 aggactgtgc cgaggcggcc gtggacggcc tggccaccga gcgcttcctg atcctgcccc
     1561 accctgaggt ggccgagttc gtgctccgca aggcgaccga ccacgatcgc tggttggccg
     1621 ggatgcgccg actgcaggcg accatcgagg gaggcctgtc gtgaggcagg ccaagaagct
     1681 cttgggcgtc ctgttcccgc tgctgctcat cggcgggttc gcgatcatcg gtgccgtctc
     1741 gggcgacgtc gatgacggct ccggctccga caagtccttc gacctcgagt cgatcgacgt
     1801 gaacaccgcg ctgcgccgtg acggtgtcct cctcgtgagc gaggaggtga cctacgactt
     1861 cgacggcacg ttcaacgtcg gcacccgcga cttcgacgcc gggccctggg ccatcaccaa
     1921 catccgggcc tacgaggacg accagccgtt ggacaccatc accaacacgc ccaccctgtt
     1981 cgagtgggac ctctccgccg cctcgggcac ccacacctac gagctgcgct acgaggtccg
     2041 cgacgccgtc atcgcctggc ccgatgtggt cga
//
LOCUS       DASTFV010000102         5190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_339434, whole genome shotgun sequence.
ACCESSION   DASTFV010000102 DASTFV010000000
VERSION     DASTFV010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339434"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VFP02_01850"
     CDS             <1..206
                     /locus_tag="VFP02_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HET9541795.1"
                     /translation="LSAGINSDKSRDINKFLKALGIKGLTSQTQGEQVRVSHKKRDAL
                     QEAMAALKEEDFGIPLQFQNFRD"
     gene            complement(203..1045)
                     /locus_tag="VFP02_01855"
     CDS             complement(203..1045)
                     /locus_tag="VFP02_01855"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET9541796.1"
                     /translation="MKNTLKTFTLLAALGGLFLFIGSFWGQGGLIIGLVLGLVVVGGS
                     YWFSDTIAIKAARAQPVSEAEMPELYRIVRELTTLADLPMPKLYVTPERQPNAFATGR
                     NPDHAAVAVTAGILEALSWDELRGVLAHEISHIGNRDILIGSVAAAVALAITFVARMA
                     MWASIFGGGDRDRNVFGELALILLAPLAAMLLQMALSRSREFEADRTGAELIGTGEPL
                     ASALQKLDAYAQRIPADIRPEQAAAYIVNPLRGRRVSFANAFASHPPMEERIARLRQP
                     VDAR"
     gene            complement(1089..1574)
                     /locus_tag="VFP02_01860"
     CDS             complement(1089..1574)
                     /locus_tag="VFP02_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541797.1"
                     /translation="MEVPENYWWRDGPVAPGPAVVFDMDGVLSDASTRQHYLEFPSRD
                     WEAFFAACGEDELVDEVARLLECLDDGLHIVLLTARPMRVQRQTLGWLERYKLRWDLL
                     IMREFGDYQASRAFKQRTVGELRGYGFDLRLAFEDDRRNVDMFHREGVPCVYIHSGYY
                     D"
     gene            complement(1598..3556)
                     /gene="acs"
                     /locus_tag="VFP02_01865"
     CDS             complement(1598..3556)
                     /gene="acs"
                     /locus_tag="VFP02_01865"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HET9541798.1"
                     /translation="MAAEPTIEDYLREERTFSPPASFTADALVTDRSLYDEADADYEA
                     FWARQARELLTWSKDFTTVLEWDLPVARWFGDGELNVAYNCLDRHVEAGKGDKVAFHW
                     EGEPGDTRSVTYADLLDEVCRFTNVLKGLGVVKGDRVAISMPMIPELPVAMLSCARLG
                     AAHSVVFGGFSPDSLIDRINDAEAKVAVTADGGWRRGSASGLKVNVDAALDATPSITD
                     VVVVRRTGQDVDMVEGRDHWYHDLMADASADCPAEPMEAEQLLYLLYTSGTTAKPKGI
                     MHTTGGYLTQVAFSHKVVFDLHPETDVYWCAADIGWVTGHSYIVYGPLTNGATSVMYE
                     GTPDHPHKARLWEIVEKYGVTQLYTAPTAIRTFMKWGPDEPASKDLSSLRLLGTVGEP
                     INPEAWMWYHQHIGGERCPIVDTWWQTETGGHMITPLPGVTTTKPGSATFPLPGIGAE
                     LVDEAGNPVAQGGGYLTLTRPWPAMLRGIWGDPERYRETYWSRFEGRYFAGDGAKIDE
                     DGYLWLLGRVDDVMNVSGHRISTTEVESALVSHPAVAEAAVVGAADDTTGQAIIAYVI
                     LRGGNEPTPELGEELRQHVAKKIGPTARPKTAILVPDLPKTRSGKIMRRLLKDVAEGR
                     DLGDTTTLADPAVFDEIRAKADEAPPED"
     gene            complement(3566..4096)
                     /locus_tag="VFP02_01870"
     CDS             complement(3566..4096)
                     /locus_tag="VFP02_01870"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HET9541799.1"
                     /translation="MTEAPAPRILPPTVLEGAAELDGAVDALRALVDDALPPGDVRDA
                     LQGKWLGHPVHPVLTDLAIGFWTSAFVLDLVPVKRLRAAADVFVALGLAAALPTVATG
                     LADWSRLPRERQRVGVVHAGSNAVASGFYAWSLAARVRGRRTRGIALGMLGAGAATVG
                     GYLGGHLAFPPSETGA"
     gene            complement(4162..4341)
                     /locus_tag="VFP02_01875"
     CDS             complement(4162..4341)
                     /locus_tag="VFP02_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541800.1"
                     /translation="MIRALIVGLAVFLIATFPATWLLMLFLGNLGLGLSYWGALPLGI
                     LVSALIGGSSANTYA"
     gene            complement(4381..>5190)
                     /locus_tag="VFP02_01880"
     CDS             complement(4381..>5190)
                     /locus_tag="VFP02_01880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET9541801.1"
                     /translation="EVGPLFGAVLVRALDAWWDAQGRPAAFTVYDAGSGAGTLGRSLA
                     LAGSPHRYVAVDRSRAQRARHQGLESAADLPDGPLVGVVVANELLDNLPFDLTVGGRP
                     FDPGQNWPETGLAPRQDQARQWVEDVLARLRGRLVVVDYVSTTAAMATRPWPEWLRTY
                     RGHDRGGHPMDHLGAQDITTEVAVDQLPAPDEDHSQADWLRSWGIDDLVEEGRRIWHE
                     RAHLGDLGAIQARSRVNEAEALLDPAGMDSFRVLEWSAVEEAGERDPGGAV"
BASE COUNT          758 a         1786 c         1902 g          744 t
ORIGIN      
        1 cgctgagcgc gggcatcaac tccgacaagt cccgggacat caacaagttc ctgaaggccc
       61 tcggcatcaa gggcctgacc agccagaccc agggcgagca ggtccgggtc tctcacaaga
      121 agcgggatgc cctccaggag gcgatggcgg ccctgaagga ggaggacttc ggcatccccc
      181 tccagttcca gaacttccgg gactagcggg cgtccaccgg ctggcggagg cgggcgatcc
      241 gctcctccat gggcgggtgg ctggcgaagg cgttggcgaa gctcacccga cggccgcgca
      301 gcgggttgac gatgtacgcc gccgcctgct cgggccggat gtcggcgggg atgcgctggg
      361 cgtaggcgtc cagcttctgg agggcagagg ccagcggctc cccggtgccg atcagctcgg
      421 cgccggtgcg gtccgcttcg aactcccggc ttcgggagag ggccatctgc agcagcatgg
      481 ccgccagagg cgccaggagg atcagggcca gctccccgaa cacgttgcgg tcccggtcgc
      541 cgccgccgaa gatcgacgcc cacatcgcca tgcgggccac gaaggtgatg gccagggcca
      601 ccgcggcggc cacggacccg atcaagatgt cgcggttgcc gatgtggctg atctcgtggg
      661 ccaggacgcc gcggagctcg tcccaggaga gggcctcgag gatgccggcg gtcaccgcca
      721 cggcggcgtg gtccgggttg cgcccggtgg cgaaggcgtt gggctggcgc tccggggtga
      781 cgtacagctt cggcatcggc aggtcggcga gcgtggtgag ctcccgcacg atgcggtaca
      841 gctcgggcat ctcggcctcg gacacgggct gcgcccgggc ggccttgatg gcgatggtgt
      901 cggagaacca gtacgagccg ccgacgacca cgaggcccag gacgaggccg atgatcaggc
      961 ccccctggcc ccagaacgag ccgatgaaca ggaagaggcc gccgagggcg gcgagcaggg
     1021 tgaaggtctt cagggtgttc ttcatggcag tcggaacaac gttcgagggg cgccgcgcgt
     1081 tcccgcgctc agtcgtagta accgctgtgt atgtagacgc agggcacgcc ctcgcggtgg
     1141 aacatgtcca cgttgcggcg gtcgtcctcg aaggccaggc ggaggtcgaa gccgtagccc
     1201 cggagctcgc cgacggtgcg ctgcttgaag gctcgggagg cctggtagtc gccgaactcc
     1261 cgcatgatga gcaggtccca gcgcagcttg tagcgctcca gccatcccag ggtctggcgc
     1321 tggacccgca tcgggcgggc cgtcagcagg acgatgtgca ggccgtcgtc gaggcactcc
     1381 aggaggcggg ccacctcgtc cacgagctcg tcctcgccgc aggcggcgaa gaaggcctcc
     1441 cagtccctgc ttgggaactc gaggtagtgc tgacgggtgg aggcgtcgga gaggaccccg
     1501 tccatgtcga acaccacggc gggccccggc gccaccggcc cgtcccgcca ccagtagttc
     1561 tccgggacct ccacctccgg gaggctaggg ctgttcctca atcctccggg ggggcctcgt
     1621 cggccttcgc ccggatctcg tcgaagacgg cggggtcggc cagggtggtg gtgtcaccga
     1681 ggtcgcggcc ctcggccacg tccttcagca ggcggcgcat gatcttgccg ctgcgggtct
     1741 tgggcaggtc ggggacgagg atggcggtct tcggccgggc ggtggggccg atcttcttgg
     1801 ccacgtgctg gcgcagctcc tcgcccagct ccggcgtcgg ctcgttcccg ccccgcagga
     1861 tcacgtaggc gatgatcgcc tggccggtgg tgtcgtcggc ggccccgacg acggcggcct
     1921 cggccaccgc cgggtgcgac accagggccg actccacctc ggtggtcgag atgcggtggc
     1981 cggacacgtt catgacgtcg tccacccggc ccaggagcca gaggtaaccg tcctcgtcga
     2041 tcttggcccc gtcgccggcg aagtaccggc cctcgaagcg gctccagtag gtctcccgat
     2101 agcgctcggg atcaccccag atgcctcgca gcatggcggg ccacgggcgg gtcagggtga
     2161 ggtagccccc gccctgggcc accgggttgc cggcctcgtc gaccaactcg gccccgatcc
     2221 cgggcagggg gaaggtggcc gagcccggct tggtcgtcgt gacgcccggc aggggggtga
     2281 tcatgtgccc accggtctcg gtctgccacc acgtgtccac gatcgggcag cgctcgccac
     2341 cgatgtgctg gtggtaccac atccaggcct cggggttgat gggctccccc acggtgccca
     2401 gcaggcggag cgaggagagg tccttgctgg cgggctcgtc ggggccccac ttcatgaacg
     2461 tccgaatggc ggtgggggcc gtgtagagct gggtcacgcc gtacttctcg acgatctccc
     2521 acagccgggc cttgtgcggg tggtccggcg tgccctcgta catgaccgac gtggccccgt
     2581 tggtcagcgg cccgtagacg atgtagctgt ggccggtgac ccagccgatg tccgccgcgc
     2641 accagtagac gtcggtctcc gggtggaggt cgaagaccac cttgtgggag aaggccacct
     2701 gggtcaggta gcccccggtg gtgtgcatga tccccttggg cttggccgtg gtccccgacg
     2761 tgtacagcag gtacaggagc tgttcggcct ccatcggctc ggccgggcag tcggcactcg
     2821 cgtcggccat gaggtcgtgg taccagtggt cccggccctc gaccatgtcg acgtcctggc
     2881 ccgtgcgccg gaccacgacc acgtcggtga tcgacggcgt ggcgtcgagg gcggcgtcga
     2941 cgttgacctt cagcccggac gcactccccc gccgccaacc cccgtcagcg gtgaccgcga
     3001 ccttggcctc agcgtcgttg atgcggtcga tgagcgagtc gggcgagaac ccgccgaaga
     3061 ccaccgagtg ggcggcgccg aggcgggcac aggagagcat ggccacgggc agctcgggga
     3121 tcatcggcat ggagatggcg acccggtccc ccttcacgac gcccagcccc ttgagcacgt
     3181 tggtgaaccg gcagacctcg tcgagcaggt cggcgtaggt gacgctgcgg gtgtcacccg
     3241 gctcgccctc ccagtggaag gcgaccttgt cgcccttgcc ggcctccacg tggcggtcga
     3301 ggcagttgta ggcgacgttc agctcgccgt ccccgaacca ccgggcgacg ggcaggtccc
     3361 attccaggac cgtcgtgaag tccttcgacc aggtcagcag ctctcgggcc tgccgggccc
     3421 agaaggcctc gtagtcggcg tcggcctcgt cgtagagcga gcggtcggtc accagggcgt
     3481 cggcggtgaa cgaggcgggc ggtgaaaagg tccgctcctc ccggaggtag tcctcgatgg
     3541 tgggttcagc ggccatggtt cgaccttacg cgccggtctc agacggcggg aaggccagat
     3601 ggccaccgag gtagccaccg acggtggcgg cccccgcgcc cagcatcccc agggcgatgc
     3661 ctcgggtccg ccgaccccgg acccgagccg cgagtgacca ggcgtagaag ccggacgcca
     3721 cggcgttgct cccggcgtgg accacgccca cccgctgccg ctcccgaggc agccggctcc
     3781 agtcggccag gcccgtggcg acggtcggca gggccgccgc caagccgagg gccacgaaga
     3841 cgtcggccgc ggcgcgcagg cgcttcaccg ggaccaggtc gagcacgaac gcgctggtcc
     3901 agaacccgat ggccaggtcg gtgagcacgg ggtggacggg gtggccgagc cacttgccct
     3961 ggagggcgtc gcgtacgtcg cccggtggga gggcgtcgtc caccagggcg cgtagcgcgt
     4021 cgaccgcgcc gtcgagctca gccgcgccct ccaggacggt cggcgggagg atgcgtggtg
     4081 ctggggcctc ggtcaccccc ggctcctacc cggtcgaggc ggtccgcgtt cccaccctgg
     4141 ccgacacccg gtgtccggcg gtcaggcgta ggtgttggcg ctgctaccgc cgatgagggc
     4201 ggagaccagg atcccgaggg gcagcgcgcc ccagtaggac agcccgaggc ccaggttgcc
     4261 caggaacagc atgagcaacc aggtggccgg gaaggtggcg atcaggaaca ccgccagccc
     4321 gacgatgagt gcacgaatca tggtgaatcc ccctcgttcc ggcgccgagt gtggcacatg
     4381 tcagaccgcg cctccgggat ccctttctcc ggcctcttcg actgcagacc actcgaggac
     4441 ccggaacgag tccatgccgg cggggtcgag cagggcctcg gcctcattga cccgactgcg
     4501 cgcctggatg gcccccaggt cgccgaggtg ggcccgctcg tgccagatcc gccgaccttc
     4561 ctccaccagg tcgtcgatgc cccacgaccg gagccagtcg gcctggctgt ggtcctcgtc
     4621 gggcgccggg agctggtcga cggcgacctc ggtggtgatg tcctgggcgc cgaggtggtc
     4681 catcggatgg ccgccccggt catgaccccg ataggtgcgc aaccactcgg gccaggggcg
     4741 ggtggccatg gcggccgtcg tcgagacgta gtcgacgacg accaggcgac cgcgcagccg
     4801 ggccaggacg tcctcgaccc attgccgggc ctgatcctgc cgcggggcga ggcccgtttc
     4861 gggccagttc tgtccgggat cgaagggccg accgccgacc gtcaggtcga acggcaggtt
     4921 gtccagcagc tcgttggcga cgaccacacc gacgagcggc ccgtcgggca gatcggccgc
     4981 cgactcgagg ccctggtgcc gggcccgctg ggccctcgaa cggtcgacgg ccacgtagcg
     5041 gtgggggctg ccggccagcg ccaggctgcg gcccagcgtg cccgccccgg acccggcgtc
     5101 gtacacggtg aaggcggccg gtcgaccctg cgcatcccac caggcgtcca gcgcgcggac
     5161 gagcacggcc ccgaagaggg gcccgacctc
//
LOCUS       DASTFV010000103         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_341608, whole genome shotgun sequence.
ACCESSION   DASTFV010000103 DASTFV010000000
VERSION     DASTFV010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341608"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFP02_01885"
     CDS             <1..543
                     /locus_tag="VFP02_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541802.1"
                     /translation="HRQASDRVAELCGLLEEIEGRLANLGENIGAQEDLVAAAAEGMA
                     TTEERLAAGPEAGAVDDAGAALDDLLLGMTEVDDTEWYLLTRLAGLRALSFAGSVPLV
                     LDGALADLDREAAHRVLDKVEAMTDVVQVIYLGDDDAVVEWAALRGSEATVITATTGD
                     AATADTSTPEAAAPVAVPVA"
     gene            598..1713
                     /locus_tag="VFP02_01890"
     CDS             598..1713
                     /locus_tag="VFP02_01890"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541803.1"
                     /translation="MTLLSDDGIRELAAFRPDQGVVTSCYLDVDGRRFPRRAEYEQEL
                     DRVLRHARTRANGTSPINDDLVAIERHVKRGLDRSRTRGVAMFSCAADGFWTALALPV
                     PVRTQLVVGHAPAVGQLEAALHHHEPIGVLLVDRQQARVLIFEMGELTEHAEEVGDLL
                     RAVDEKDEKARGDGSSRVEDRDRQHVRRAAAAAFGVYQRHPFEHLLVGAPAALIGAVE
                     DALHPYLRERLRERTTLAVGDPVETIRAAVMAAETDLERRREAGLIDELRGRLGRDDR
                     ATAGLATVLEALADRRVERLLVSGGYHEEGWSCAGCGTLAKVGPTCPRCGTAMREEQD
                     VVAAAIDDALAASCRVDVCDRSADLDVLGRIGALLRF"
     gene            1719..2675
                     /locus_tag="VFP02_01895"
     CDS             1719..2675
                     /locus_tag="VFP02_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9541804.1"
                     /translation="MPTPTTIGVDVGGTKLLALALDTTNDAVLGTARHPTPRGAEALL
                     DGVAEIVAEITEQCTLGPDLPVGVGVAGLVDHRGVLRHGPNQHGVRELEVLGGLVDRL
                     GRRVLVDNDGNCAVWAEWSSGAARGQDDVVLVTLGTGIGAGLMVGGQVVRGANGMAGE
                     PGHTMVDPTGPPCPCGRRGCWERYASGAGLARMARDAAEAGRLEEVLAAAGGDAAAIR
                     SEDVVTAGRTGDPEATAIMARFAWWTAVGVANLVAVLDPDVVVLGGGLIEAADLWLDE
                     TRRRLPEVLVASEHRVLPRVEAAVHGTEAAAMGAALLARDPA"
     gene            2719..>2985
                     /locus_tag="VFP02_01900"
     CDS             2719..>2985
                     /locus_tag="VFP02_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9541805.1"
                     /translation="MEFRRITSLPPYVFTIIDGLKIELRREGVDVVDLGFGNPDLPSP
                     EVAVAKLTEAAQNPRNHRYSSSRGIPKLRQAVAALYERRFGVTLD"
BASE COUNT          405 a         1103 c         1087 g          390 t
ORIGIN      
        1 caccgacagg ccagcgaccg ggtcgccgag ctctgcggcc tgctcgagga gatcgaaggt
       61 cgcctggcca acctgggcga gaacatcggg gctcaggagg acttggtggc ggcggccgcc
      121 gaaggcatgg ccaccaccga ggagcgcctg gctgccggcc cggaggccgg tgccgtcgac
      181 gatgccggcg ccgcgctgga cgacctgctc ctcggcatga ccgaggtgga cgacaccgag
      241 tggtatctcc tcacccgcct cgccggcctc cgggcgctct cgttcgccgg atcggtcccc
      301 ctcgtcctcg acggggccct ggctgatctc gaccgtgagg ccgcccatcg ggtcctcgac
      361 aaggtcgagg cgatgaccga cgtggtccaa gtcatctacc tgggcgacga cgacgccgtc
      421 gtcgaatggg ccgccctgcg cgggtccgag gcgacggtca tcaccgccac gaccggcgac
      481 gccgccaccg cggacacctc cacgccggag gccgccgcgc cggtcgccgt ccccgtcgcc
      541 tgaccgctcg ccgaggactc ggctgacgcc gggcacccac cgccctaccc tgggcggatg
      601 accctcctga gcgacgacgg gatccgcgaa ctggccgcct tccgccctga ccagggcgtg
      661 gtcacgagct gttacctcga cgtagacggg cgccgcttcc cgcgccgggc cgaatacgaa
      721 caggagctcg accgcgtgct ccgccacgcc cgcacccggg ccaacggcac atcaccgatc
      781 aacgacgacc tcgtggccat cgagcgccac gtcaaaaggg gcctggaccg ttcccggacc
      841 cgcggcgtgg cgatgttctc gtgcgcggcg gacggcttct ggaccgccct cgccctgccg
      901 gtgccggtac ggacccagct cgtcgtgggc catgccccgg ccgtcggcca gctggaggcg
      961 gccctgcacc atcacgaacc catcggcgtg ctgctggtcg accggcagca ggcccgcgtc
     1021 ctcatcttcg agatggggga gctgaccgag cacgccgagg aggtcggcga cctgctgcga
     1081 gccgtcgacg agaaggacga gaaggcccgg ggcgacgggt cgtcccgggt ggaggaccgg
     1141 gaccggcagc atgtccgccg cgccgctgcg gcggccttcg gcgtgtacca gcgccacccc
     1201 ttcgagcacc tgctcgtcgg ggcccccgcg gcgctgatcg gggccgtgga ggacgccctc
     1261 cacccctacc tgcgggaacg tctccgcgag cggacgaccc tcgccgtcgg cgacccggtc
     1321 gagaccattc gggccgcggt catggccgcc gagacggacc tggagcgccg ccgggaagcg
     1381 ggactcatcg acgagctgcg gggccgactc ggccgcgacg accgggcgac cgccggcctg
     1441 gccacggtgc tcgaggcctt ggccgaccgg cgggtcgagc ggttgctggt gtcggggggt
     1501 taccacgagg agggctggtc ctgcgccggc tgtggcacct tggccaaggt cggcccgacc
     1561 tgccctcgct gcggcaccgc gatgcgcgaa gagcaggatg tcgtggccgc cgccatcgac
     1621 gacgcgctgg ccgcctcgtg ccgggtcgac gtctgcgacc gctccgccga cctcgatgtg
     1681 ctcggccgta tcggcgcctt gctccgattc tgagcaccat gcccaccccg accacgatcg
     1741 gggtcgatgt cggcgggacc aagctgctgg ccctcgccct cgacaccacg aacgacgccg
     1801 tcctcggtac ggcccgacac cccacccccc ggggagcgga ggcgctcctc gacggggtgg
     1861 ccgagatcgt ggcggagatc accgagcagt gcaccctcgg accggacctg ccggtgggcg
     1921 tcggcgtggc cggcctggtc gaccaccgag gcgtcctgcg tcacggtccc aaccagcacg
     1981 gcgtgcggga actggaggtc ctcggaggct tggtcgaccg cctcgggcgc cgggtgctgg
     2041 tcgacaacga cgggaactgc gccgtgtggg ccgaatggag ctcgggggcg gcccggggcc
     2101 aggacgacgt cgtcctggtc acgctcggca cgggcatcgg tgccggactg atggtgggtg
     2161 gccaggtggt gcgcggggcc aacgggatgg ccggggaacc gggtcacacc atggtcgacc
     2221 cgaccggtcc accgtgcccc tgcgggcgcc gcggatgttg ggagcgttat gcctccggtg
     2281 ccggcctggc ccggatggcc cgcgacgcgg cggaagccgg ccgcctggaa gaggtgctgg
     2341 cagcggcggg cggcgacgcc gcagccatcc gcagtgagga cgtcgtcacg gcaggccgga
     2401 cgggcgaccc ggaggcgacg gcgatcatgg cccgcttcgc ctggtggacc gcggtcggcg
     2461 tggccaacct cgtggccgtc ctcgaccccg acgtggtggt cctgggcggc ggcctgatcg
     2521 aggccgccga cctctggttg gacgagaccc ggcgccggtt gcccgaggtg ttggtggcct
     2581 ccgagcaccg cgtcctaccg cgcgtggagg ccgccgtcca cggaacggag gcggcggcca
     2641 tgggcgccgc actcctcgcc cgcgatccgg cctgaagtcc ccgagggcag ccggacggcg
     2701 ccgctaccct cgaagtcgat ggagttccgc cggatcacgt cgctgccgcc gtacgtgttc
     2761 acgatcatcg acgggctcaa gatcgagctc cgccgggagg gggtggacgt ggtcgatctc
     2821 gggttcggga accccgacct gccgtcgccg gaggtggccg tcgccaagct caccgaggcc
     2881 gctcagaacc cccgcaacca ccgctactcg tccagccggg gcatccccaa gctgcgccag
     2941 gccgtcgccg cgctctacga gcgacgcttc ggcgtcaccc tcgac
//
LOCUS       DASTFV010000104         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_346378, whole genome shotgun sequence.
ACCESSION   DASTFV010000104 DASTFV010000000
VERSION     DASTFV010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346378"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /gene="rpmA"
                     /locus_tag="VFP02_01905"
     CDS             <1..208
                     /gene="rpmA"
                     /locus_tag="VFP02_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET9541806.1"
                     /translation="TSKRLGVKVYDGTVVNAGAIIVRQRGTKIHPGDNVGRGGDDTLF
                     ATANGTVKFGARKGRKHVSVLSEG"
     gene            254..1324
                     /locus_tag="VFP02_01910"
     CDS             254..1324
                     /locus_tag="VFP02_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9541807.1"
                     /translation="MSTSPSRWPDVAWAHAPLAVGEVTSVARREREEREHALLAPGAT
                     RASGAGSRARAEDPDPNRTCFERDLDRIQHSRPFRRLAGKCQVYLAPDDDHLRTRLTH
                     AIEVGQVATSIARATRLNEALTLASALGHDCGHGPAGHASEDALSPYLEGGYHHAVYG
                     ADVVLEPLNLCAETLDAVRNHSWNRPAPATPEGEVVAWADRIAYVCHDFEDAVDAGVV
                     TAAELPTEVVEAVGDRRSAQLGGFIAAMVETIAETGRIGMRKPEADALAAFRAFNYER
                     IYLRPESVAQADRVNAMLRQLTEWFIENPRAIGAGEILRPGSAAAAAAAVRYVSGMTD
                     RFALRLAVDRLGWSEADLPTGV"
     gene            1376..2638
                     /gene="obgE"
                     /locus_tag="VFP02_01915"
     CDS             1376..2638
                     /gene="obgE"
                     /locus_tag="VFP02_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET9541808.1"
                     /translation="MAGFVDEAQLCARAGDGGAGCVSFRREAHVSRGGPDGGDGGTGG
                     DVWLVATRNVASLLAFRDHPHRRAENGVHGQGKKKHGAAGADIDVPVPEGTQVKDRDG
                     ILLADLAHAGDRWRAVEGGRGGRGNARFLSNRRRAPAFAEQGEPGDEIWFDLELKLLA
                     DVALVGFPNVGKSTLIARISAARPKIADYPFTTLEPNLGVVRTDDAEFVVADIPGLIE
                     GASEGKGLGHQFLRHIERARVLVILCDLAPADGVPVAEQQRILLDELGQYQPDLLDRP
                     RLVVGSRADMVTDDAIPEGLDLIISSITGRGIDDLVGRAATLVTGARAAEPVDEEESR
                     GPAFVLHRPGAAPFDIFRDDDGAYVVDGREARRAVALSDLSDVQALEVAHGRLKRLGV
                     DRALARAGAEAGDTVRIGGLAFDYEPES"
BASE COUNT          377 a         1007 c          925 g          354 t
ORIGIN      
        1 caccagcaag cgcctcggcg tgaaggtgta cgacggcacc gtcgtcaacg ccggcgccat
       61 catcgtgcgc cagcgcggca ccaagatcca tcccggcgac aacgtcggtc gcggcgggga
      121 cgacaccctg ttcgccaccg caaacggcac cgtgaagttc ggtgcccgca aggggcgcaa
      181 gcacgtcagc gtgctctccg agggttagcg gtcgctctgc tcactgtccc accccttccg
      241 tagggtggac gatgtgagca ccagtccgag ccgctggccc gatgtggcct gggcccatgc
      301 cccgctcgcc gtcggcgagg tcacgtccgt ggcccgccgt gaacgggagg agcgggagca
      361 cgccctgctc gcccccgggg cgacccgcgc gtcgggcgcc ggatcccgcg cgagggccga
      421 ggaccccgat cccaaccgga cctgcttcga gcgggacctc gaccgcatcc aacacagccg
      481 gcccttccgg cgcctggccg gcaagtgcca ggtctacctg gcgcccgacg acgatcacct
      541 gcggacccgc ctgacccacg ccatcgaggt gggtcaggtg gccacgtcca tcgcccgggc
      601 cacccgcctg aacgaggccc tgaccctggc ctccgccctg ggtcacgact gcggccacgg
      661 tccggccggt cacgcgtccg aggacgccct gtcgccgtac ctggagggcg gctaccacca
      721 tgccgtctac ggcgccgacg tcgtgctcga gcccctgaac ctgtgcgccg agacgttgga
      781 cgccgtgcgc aaccactcgt ggaaccgccc ggccccggcg acgccggagg gcgaggtcgt
      841 ggcctgggcc gatcgcatcg cctacgtctg ccacgacttc gaggacgccg tcgacgccgg
      901 ggtggtcacc gccgccgaac tgcccaccga ggtcgtcgag gcggtagggg accgacgcag
      961 tgcccagctg ggcggcttca tcgcggccat ggtcgagacc atcgccgaga ccggtcggat
     1021 cggcatgcgc aagcccgagg ccgacgcgct ggcggcgttc cgggccttca actacgaacg
     1081 catctacctc cgccccgagt ccgtggccca ggccgatcgg gtcaacgcga tgctgcggca
     1141 gctcaccgaa tggttcatcg agaacccgag ggccatcggt gccggagaga tcctccgccc
     1201 gggctccgcc gcggccgccg cggccgccgt ccggtatgtc agcggcatga ccgaccgctt
     1261 cgccctgcgg ttggccgtcg atcgcctcgg ctggtcagaa gccgacctcc ccaccggcgt
     1321 ctgaccctcc ggaaccaacc ccgggggccg acgagcctgc tcgtatcctc ctggcgtggc
     1381 cgggttcgtc gatgaagcac agctctgtgc ccgcgccggt gacggcgggg cggggtgtgt
     1441 gtcgttccgg cgggaggccc atgtgtcccg gggcgggccc gacggcggcg acgggggcac
     1501 gggtggtgac gtctggctgg tcgccacccg caacgtggcc tccctgctgg ccttccggga
     1561 ccatccccac cggcgggccg agaacggcgt ccacggccag ggcaagaaga agcacggcgc
     1621 cgccggcgcc gacatcgacg tacccgtgcc cgagggcacc caggtcaagg accgcgacgg
     1681 gatcctgctg gccgacctcg cccacgcggg ggaccggtgg cgggccgtcg agggcggtcg
     1741 gggcgggagg ggcaacgccc gcttcctgag caaccgccgc cgggcgcccg ccttcgccga
     1801 gcagggcgag ccgggcgacg agatctggtt cgacctcgag ctcaagctgc tggctgacgt
     1861 cgccctggtc ggcttcccca acgtgggcaa gagcacgctc atcgcccgca tctccgccgc
     1921 ccgacccaag atcgccgact acccgttcac caccctggag cccaacctcg gcgtggtccg
     1981 caccgacgac gccgaattcg tcgtggccga catccccggc ctcatcgagg gcgcgagtga
     2041 gggcaagggc ctcgggcacc agttcctgcg gcacatcgaa cgggccaggg tcctggtcat
     2101 cctctgcgac ctggcaccgg ccgacggcgt acccgtggcc gagcagcagc ggatcctgct
     2161 ggacgaactg gggcagtacc agcccgacct cctcgaccga ccccggctcg tggtcggctc
     2221 ccgggccgac atggtcaccg acgatgccat tcccgagggc ctggacctga tcatctcctc
     2281 gatcaccggc cggggcatcg acgacctggt cggacgggcg gccacgctcg tcacgggggc
     2341 tcgcgccgcg gagccggtcg acgaggagga gtcccgcggc cccgccttcg tcctccaccg
     2401 ccccggcgcc gcgcccttcg acatcttccg ggacgacgac ggtgcctacg tggtcgacgg
     2461 tcgggaggcc cggcgggccg ttgccctctc cgacctgagc gacgtccagg ccctggaggt
     2521 ggcgcatggg cgcttgaagc gcctcggcgt cgaccgggcc ctggctcggg ccggagcgga
     2581 ggccggcgac accgtccgca tcggtggcct ggccttcgac tacgagcccg agagctgacc
     2641 gtgatcgtcg tcgtcaagat cgg
//
LOCUS       DASTFV010000105         2981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_349287, whole genome shotgun sequence.
ACCESSION   DASTFV010000105 DASTFV010000000
VERSION     DASTFV010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349287"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VFP02_01920"
     CDS             <1..693
                     /locus_tag="VFP02_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HET9541809.1"
                     /translation="RQEKKDRNALITARQDEPVAPVEDIVSADQPTGVGDDVRFRRAT
                     ATGTYDEAAQVLVRNRSFDEAPGSWVLTPLVMDDGTVLVVNRGWVPVTGEQTLSHDAA
                     PPEGTVEVEGLLEASQSRGSFGPTDPSDEVLDTLSRVDLDRYQEQVDGDLYPVWLQLE
                     SQDPPPGDRPTPVDPPELTEGPHLGYAFQWFTFTVIALVGYPLILRRRARQVDEPDET
                     AEVHQDEPVPTS"
     gene            690..1265
                     /locus_tag="VFP02_01925"
     CDS             690..1265
                     /locus_tag="VFP02_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9541810.1"
                     /translation="MSSRDQALEATLTCIANGGMKGFTVEDVARQAGLSRATIYRYFP
                     GGRDQLVTETVTWEVGRFFVRLAEAIADAPDFRTRVEQGLAFAHRSLRDHEAFQRAMA
                     TDQGEIRARLAGIAPIVVGVIRSYLEPLLEAETLVDGVTPIEAADHLARLIGSLIVDE
                     GVWDLGDPSQVHELVDRWLLSGIVVIDLTGS"
     gene            1506..2087
                     /locus_tag="VFP02_01930"
     CDS             1506..2087
                     /locus_tag="VFP02_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9541811.1"
                     /translation="MSTGPERVADAALACIADHGVRAVTVERIAVEAGVSRASVYRWF
                     PGGRNDVLLAAGRHEMTRFFTEVEPRLAAAGTLDDVLAEALSGAVRFLRDSKPLHALL
                     ANEPELVMPHLAFDRMGTAFAVAAEVAERHLRRFLDEDDACRATELIARLALSHTLSP
                     SADLDVGDLDAARRHVHRFVTPGLVPLTDCPRS"
     gene            2110..>2981
                     /locus_tag="VFP02_01935"
     CDS             2110..>2981
                     /locus_tag="VFP02_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9541812.1"
                     /translation="MSSNEALIGRADVNDLEAILAVSNTDRNEVIHAVEDNADAIFTW
                     DYAKGQRPALNRLYEKAKTSQWNGETDLDWSIEVDQEAVVAANAAQNGFMEQSLDLSG
                     TQMEHWSDEKWLEFGVESNNWLLSQFMHGEQGALVCTAKIVETVPWIDAKYYASTQVM
                     DEARHVEVFAKYLDTKLSGHYPINAHLRMLLDDIIQDSRWDMTYLGMQIMVEGLALAA
                     FGFMHQMTTEPLLKRLLRYVMSDEARHVAFGVLSLKEHYEGLTSAELRERQEFAFEAA
                     LRMRDRMLQQEVWER"
BASE COUNT          481 a         1098 c          979 g          423 t
ORIGIN      
        1 cgccaggaga agaaggaccg caacgccctc atcaccgccc gccaggacga gccggtcgcc
       61 cctgtcgagg acatcgtgag cgccgaccag cccaccggcg tgggcgacga cgtgcggttc
      121 cggcgggcca ccgccacggg cacgtacgac gaggccgctc aggtcctggt ccgcaaccgg
      181 agcttcgacg aagcgcccgg ctcgtgggtc ctcacgccgc tggtcatgga cgacggcacg
      241 gtcctggtgg tgaaccgggg ctgggtcccg gtgacggggg agcagacgtt gagccatgac
      301 gcggccccgc ccgagggcac cgtcgaggtg gagggccttt tggaggcctc ccagagccgg
      361 ggcagcttcg gtccgaccga cccgagtgac gaggtcctcg acaccctctc ccgtgtggac
      421 ctcgaccgct accaggagca ggtcgacggc gacctctacc cggtgtggct ccagctcgag
      481 tcccaggatc cgccacccgg tgacaggccc accccggtcg acccgcccga gctgaccgag
      541 ggcccccacc tgggctacgc cttccagtgg ttcaccttca cggtcatcgc cctcgtcggc
      601 tacccgctca tcctgcgccg tcgggcccgc caggtcgacg agcccgacga aaccgccgag
      661 gtccaccagg acgagcccgt cccgacgtca tgagctcgcg ggaccaggcg ctggaggcca
      721 cgctgacctg catcgcgaac gggggcatga agggcttcac cgtcgaggac gtggcccggc
      781 aggccggcct gtcccgggcg acgatctacc gctacttccc gggcggtcgt gaccagctgg
      841 tgaccgagac ggtgacctgg gaggtgggcc gcttcttcgt tcgcctggcc gaggccatcg
      901 ccgacgcccc ggacttccgc acccgggtcg agcagggcct ggcgttcgcc caccggtccc
      961 tgcgcgacca cgaggcgttc caacgggcca tggcgaccga ccagggcgag atccgggccc
     1021 gcctggccgg gatcgccccc atcgtggtcg gggtcatccg cagctacctg gagcccctcc
     1081 tcgaggccga gaccctggtc gacggcgtca cccccatcga ggccgccgac cacctggccc
     1141 gcctcatcgg cagcctcatc gtcgacgagg gcgtctggga cctgggcgat ccgagccagg
     1201 tccacgagct cgtcgaccgt tggctgctga gcggcatcgt cgtgatcgac ctgacgggaa
     1261 gttgagcgag cctgcgcagg tcagtcggac cagcagtggc tcctgagcgg catcgtcgtg
     1321 atcgagctgt cctgaggccc tgcaggcgcc agccggagca gcaccacgga cgagcagctt
     1381 cggcggagcc ggccagtcag atgtagatga gaccattgtc acaggccagg ccccttcccc
     1441 tgagacaaaa aggggcgtac tgtctcatcg tgatgggggc catcgcggcg ggcgaacgcc
     1501 gacacttgag cacgggaccc gagcgggtcg ccgacgccgc cctcgcctgc atcgccgacc
     1561 acggggtgcg ggccgtcacg gtcgagcgca tcgccgtaga ggccggcgtc tcccgcgcca
     1621 gcgtgtaccg ctggttcccc ggcggtcgca acgacgtcct cctggccgcc ggtcgtcatg
     1681 agatgactcg gttcttcacc gaagtggagc cccgcctcgc cgccgccggg accctcgacg
     1741 acgtcctggc cgaggccctg tccggtgccg tgcgcttcct gagggacagc aagccgctgc
     1801 acgccctcct ggccaacgag cccgagctgg tcatgcccca cctggccttc gaccgcatgg
     1861 gcaccgcctt cgccgtcgcc gccgaggtcg ccgagcgcca cctgcgccgc ttcctcgacg
     1921 aggacgacgc ctgccgggcc accgagctca tcgcccggct cgccctctcg cacacccttt
     1981 ccccatcggc cgacctcgat gtgggggacc tggatgccgc ccggcgccac gtccaccgct
     2041 tcgtgacccc cggcctcgtc cccctcaccg attgccccag gagctgacgc catgtctacg
     2101 accgaagaga tgtccagcaa cgaggccctc atcgggcggg cggacgtcaa cgacctcgag
     2161 gccatcctcg cggtgtccaa caccgaccgg aacgaggtga tccacgccgt cgaggacaac
     2221 gccgacgcca tcttcacctg ggactacgcg aagggtcagc gcccggccct gaaccggctc
     2281 tacgagaagg ccaagacctc gcagtggaac ggggagaccg acctggactg gtcgatcgag
     2341 gtcgaccagg aggccgtcgt ggccgccaac gccgcccaga acggcttcat ggagcagagc
     2401 ctcgacctct cgggcaccca gatggagcac tggtccgacg agaagtggct ggagttcggg
     2461 gtcgagtcca acaactggct gctctcccag ttcatgcacg gtgagcaggg cgccctggtc
     2521 tgcacggcca agatcgtcga gaccgtcccg tggatcgacg ccaagtacta cgcctcgacc
     2581 caggtgatgg acgaggcccg ccacgtcgag gtcttcgcca agtacctcga caccaagctg
     2641 tcgggccact acccgatcaa cgcccacctg cggatgctgc tggacgacat catccaggac
     2701 agccgctggg acatgaccta cctgggcatg cagatcatgg tcgagggcct ggccctggcc
     2761 gccttcggct tcatgcacca gatgaccacc gagccgctgc tgaagcggct cctgcgctac
     2821 gtcatgagcg acgaggcccg ccacgtcgcc ttcggcgtcc tcagcctgaa ggagcactac
     2881 gagggcctga cctccgccga actgcgcgag cgtcaggagt tcgccttcga ggccgccctg
     2941 cgcatgcggg accggatgct tcagcaggag gtctgggagc g
//
LOCUS       DASTFV010000106         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_359464, whole genome shotgun sequence.
ACCESSION   DASTFV010000106 DASTFV010000000
VERSION     DASTFV010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359464"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1123
                     /locus_tag="VFP02_01940"
     CDS             149..1123
                     /locus_tag="VFP02_01940"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET9541813.1"
                     /translation="MEALGRRHLMLFAGRASRELANEVARHLGIECGSVDIRDFANGE
                     IYARYGESVRGTDAFVIQTHSAPVNERIMEQLIMIDALKRASAKRISAVIPYYGYSRQ
                     DKKGRGREPISAKLVADMLHTAGADRLITMDLHSGQIQGFFDGPVDHLTAMPVLADHI
                     RSSCESRDLVVVAPDAGRVKTAEKMAGVLGAPLAFLHKRRSRTEAHKIEVREVVGEVD
                     GRVCVLIDDMIDTASTIVQGAEVLMASGAERVTAAATHGIFSGPAIDRLKNAPIAEVV
                     VTNTLPLGPDQKLDKMRVLSVAGIVADAIRAVFEDASVSEIFNDQNQF"
     gene            1197..1775
                     /locus_tag="VFP02_01945"
     CDS             1197..1775
                     /locus_tag="VFP02_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET9541814.1"
                     /translation="MAGELQGRKIAFLATDGVEQIEYTDPRKAVEEAGATAHLVALEP
                     GEIQGFNHLDRGDRFPVDKAVAEASADDYDGLVLPGGVANPDALRADKDAVQFVRSFF
                     EAGKPVASICHGAWTLVEADVVRDRTLTSWPSIKTDIRNAGGTWVDEEVHTDRGLVTS
                     RKPDDLPAFNAKIVEEFAEGVHEEQAQATARA"
     gene            1837..2289
                     /locus_tag="VFP02_01950"
     CDS             1837..2289
                     /locus_tag="VFP02_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9541815.1"
                     /translation="MPKTARDVMTPNAECVGENETLLDAARKMADRGYGSMPICGEDS
                     RLKGMLTDRDIVVKALAQGRDPASTTAGELAEGKPVTIGADDSLQDALKTMAQYKVRR
                     LPVIDGHDLVGIIAVADVARELDDDDSKGDLIEAISAGPAQDGGPGSQ"
     gene            2354..>2478
                     /locus_tag="VFP02_01955"
     CDS             2354..>2478
                     /locus_tag="VFP02_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET9541816.1"
                     /translation="MLAKSVSILPEGMLYEPKWDGFRCVVFRDGDEVELGSRKERP"
BASE COUNT          438 a          862 c          877 g          301 t
ORIGIN      
        1 ccatcaccaa ggacgtgccg cccgacgcac tggccatcga acgctccgac caacggacga
       61 tcgagggctg ggcggccagg cgccgacggc ggaagaccag ggagtagacg gggagggcac
      121 ggtgagcaac ggtgtgacgc ccaggcaggt ggaggcgctt gggcgcaggc acctgatgct
      181 gttcgcggga cgggccagcc gcgagctggc caacgaggtg gccaggcacc tcgggatcga
      241 gtgcggttcg gtggacatcc gcgacttcgc caacggcgag atctacgccc ggtacgggga
      301 gagcgtgcgc ggcaccgacg cgttcgtgat ccagacccac agcgcgccgg tgaacgagcg
      361 gatcatggag cagctgatca tgatcgacgc cctcaagcgg gcgtcggcca agcggatcag
      421 cgccgtcatc ccctactacg gctactcccg ccaggacaag aagggccggg gccgcgagcc
      481 gatctcggcc aagctggtgg ccgacatgct gcacacggcc ggggccgacc ggctcatcac
      541 catggacctg cactccgggc agatccaggg gttcttcgac gggccggtcg accacctgac
      601 cgccatgccg gtgctggccg accacatccg cagcagctgc gagagccgcg acctggtggt
      661 ggtcgccccc gacgccggcc gggtcaagac ggccgagaag atggccggtg tgctcggcgc
      721 ccccctggcc ttcctgcaca agcggcgcag ccgcaccgag gcccacaaga tcgaggtccg
      781 cgaggtggtc ggcgaggtcg acgggcgggt gtgcgtgctg atcgacgaca tgatcgacac
      841 cgccagcacc atcgtgcagg gcgccgaggt gctgatggcc agcggcgccg agcgggtgac
      901 ggccgccgcc acccacggga tcttctccgg cccggccatc gaccggctca agaacgcccc
      961 catcgccgag gtcgtggtca ccaacaccct gcccctgggc ccggaccaga agctcgacaa
     1021 gatgcgcgtg ctgtcggtgg ccggcatcgt cgccgacgcc atccgggcgg tgttcgagga
     1081 cgccagcgtc tccgagatct tcaacgacca gaaccagttc tgaggccgtt tgcagccccc
     1141 cgaggccgtc aatgcttaga ggtgaagcat tgtcgtccct cggacaggag gagcacgtgg
     1201 caggcgagct gcaaggcagg aagatcgcgt ttctggcaac tgacggagtc gagcagatcg
     1261 agtacaccga cccccgcaag gcggtcgagg aagccggcgc caccgcgcac ctggtggcgc
     1321 tggagcccgg cgagatccag ggcttcaacc acctcgacag gggagacagg ttcccggtcg
     1381 acaaggccgt ggccgaggcc agcgccgacg actacgacgg gctggtgctg cccggcgggg
     1441 tcgccaaccc cgacgccctg cgggccgaca aggacgccgt ccagttcgtg cgctcgttct
     1501 tcgaggccgg caagccggtc gcctcgatct gccacggcgc ctggacgctg gtcgaggccg
     1561 acgtggtcag ggaccgcacc ctgacctcct ggccgagcat caagaccgac atccgcaacg
     1621 ccggcgggac ctgggtcgac gaggaggtcc acaccgaccg cggcctcgtc accagccgca
     1681 agcccgacga cctgccggcc ttcaacgcca agatcgtcga ggagttcgcc gagggcgtgc
     1741 acgaggagca ggcccaggcg accgcgcgag cctgagcggg cccgggcaaa tgcccgcgac
     1801 ctgaaggaac ccagctgagg aggagggtgc ggcgccatgc cgaagacagc ccgcgacgtc
     1861 atgaccccca acgccgagtg cgtgggggag aacgagaccc tgctcgacgc ggccaggaag
     1921 atggccgacc ggggctacgg gtccatgccc atctgcggcg aggacagccg cctcaagggc
     1981 atgctgaccg accgcgacat cgtcgtgaag gccctcgccc agggcaggga cccggcctcg
     2041 accacggccg gcgagctcgc cgagggcaag ccggtcacca tcggggccga cgactcgctg
     2101 caggacgccc tgaagacgat ggcccagtac aaggtccgcc gccttccggt gatcgacggc
     2161 cacgacctgg tcggcatcat cgccgtggcc gacgtcgccc gggagctgga cgacgacgac
     2221 tccaagggcg acctgatcga ggccatctcc gccggccccg cccaggacgg cggccccggg
     2281 tcgcagtagc ccgagctcgg ttcgtggcac catcgggggc atggacctgc ccgtcatgcc
     2341 cccgatccgc ccgatgctcg ccaagtcggt ctcgatcctg cccgagggga tgctgtacga
     2401 gcccaagtgg gacgggttcc gctgcgtggt gttccgcgac ggcgacgagg tcgagctggg
     2461 cagccgcaag gagcggcc
//
LOCUS       DASTFV010000107         4222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_360512, whole genome shotgun sequence.
ACCESSION   DASTFV010000107 DASTFV010000000
VERSION     DASTFV010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360512"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /gene="extP"
                     /locus_tag="VFP02_01960"
     CDS             <1..693
                     /gene="extP"
                     /locus_tag="VFP02_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HET9541817.1"
                     /translation="SIFRPGSIFRKGYTDSPRNRSYVIMNSVLYHLHPVKVKRHAVKV
                     SYTLCLGGLSFFLFILLTVTGIFLMFFYRPTATQAWDDIQALHTSVAFGLLVRNMHRW
                     AAHLMVISVFLHMARVFYHGAYKPPREFNWVIGVVLLLLTLLLSFTGYLLPWDQLALW
                     AVTVGSNMGGFAPVFGSQVKFALLGGAEIGSETLLRWYVLHVLFLPFVIVIFMAVHFW
                     RVRKDGGISGPL"
     gene            709..1467
                     /locus_tag="VFP02_01965"
     CDS             709..1467
                     /locus_tag="VFP02_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HET9541818.1"
                     /translation="MVEIPEHLRKRAEEARAKAEAARGGGDGGTDAGDAGGADSGIPS
                     ELLERSKAARARAAGGDADTAPAASGGGTAVATAPAAATPTGPGGHTQRLLTVVKAGS
                     IQDVKATPVDKVHVWPHLLLIEFVAALGITAFLLFFSIFVNAPLLELANPNRTPNPSK
                     APWYFLGLQELLTMFHPMIAGVTIPTIGLIGYAMVPYIDKNPSTKPEDRKFAISLFTV
                     QLMFWAVLVIIGSFFRGPGQNFVFPWNEGIFFDL"
     gene            1477..2331
                     /locus_tag="VFP02_01970"
     CDS             1477..2331
                     /locus_tag="VFP02_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET9541819.1"
                     /translation="MSTGTILLIVIPLLVVLAGILLAGAARRRETGDAVGGLSRETRK
                     RDQGVALDADLEPTGRDVEREAKLAQSGKLVPVDPAPLEPYTPPDAETLGVTRRQFLN
                     RAILGFTGFSLAAFGGAVLAFIWPNAQGGFGSKIRIGNIDDVKASIVEGNGFFYVPEG
                     RMWITEYPSDALEKAAQVYSPAELTGMEAGVNTLFQTCPHLGCRVPNCDSSQWFECPC
                     HGSRYNRVGEKRGGPAPRGMDRFPSTVSGGVLEVDTGIRIQGPPIGTNTTGQEPEGPS
                     CLGGSTHE"
     gene            2328..3380
                     /locus_tag="VFP02_01975"
     CDS             2328..3380
                     /locus_tag="VFP02_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET9541820.1"
                     /translation="MSVLAGAVLASTVSSVGYVIVVLTLIAFGVAVFINIRRARAETG
                     SELELAPNRVPHHPDEVLEGPVLNRNLAFGLGAIVIISIGLPVYWLGEPARNEGAQAT
                     FDRVAAERGAEQFAVTAEGGLNCAGCHGGMDAPGGVAPYTINDANGEFVASVEWQAPA
                     LNTVLLRFDEEELYDILVYGRPFSPMPAWGIEGGGALNDQQIGNLIAYLKTIQITPEE
                     AQAAATDALADAMASGQYETEGEALFNLGLYDGFAGGAYSCGRCHTQGWSYGEPESDG
                     DGALGPNLTAVRSQFPGGAAGFQEQVDFVTEGSVKGVLYGAHGQGTGRMPGFDDMLTD
                     EQIQAIVEYERNLGDD"
     gene            3373..>4222
                     /locus_tag="VFP02_01980"
     CDS             3373..>4222
                     /locus_tag="VFP02_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541821.1"
                     /translation="MTDSIVGVLLAGISYDPEIRGVLVVTVAALILIGSVYLLLSTNV
                     GARLGFQVAMAGLFGWLMLLSLFWWIYGVGAIGTLPSWRVEEINVGDLSQAQLEEARG
                     LVPENLPSAEDIIAEFPDVAEAMAEDPTPTLSEIAALPDLPPEVEEQLAGLEGGWDVL
                     SQAAIGDAQAAADTALISEETGLYTSASEYVLVDGFTRGGKEQPESDSLWDRVSTRVA
                     NAFTVLNPPKYTVIQVQTSESTAVPEGNAPLSPTPDEDQPVVSVIMVRDLGTRRLPPA
                     MLTILFG"
BASE COUNT          629 a         1517 c         1406 g          670 t
ORIGIN      
        1 tcgatcttcc gacccggctc gatcttccgg aagggctata cggacagccc ccggaaccgg
       61 tcctacgtca tcatgaacag cgtcctgtac cacctccacc cggtgaaggt gaagcggcac
      121 gcggtcaagg tcagctacac gctctgcctc ggtgggctga gcttcttcct cttcatcctg
      181 ctgaccgtga ccggcatctt cctgatgttc ttctaccgcc cgaccgcgac ccaggcctgg
      241 gacgacatcc aggccctgca cacctcggtg gccttcggcc tcctggtgcg gaacatgcac
      301 cggtgggccg cgcatctgat ggtgatctcg gtcttcctcc acatggcccg ggtcttctac
      361 cacggcgcct acaagccgcc ccgggagttc aactgggtca tcggcgtggt cctgctgctg
      421 ctgaccctgc tcctgagctt caccggctac ctcctgccct gggaccagtt ggcgctctgg
      481 gccgtgacgg tcgggtcgaa catgggtggg ttcgccccgg tgttcgggtc gcaggtgaag
      541 ttcgcccttc tcggtggggc cgagatcggt tcggagacgt tgctgcggtg gtacgtgctg
      601 catgtgctgt tcctgccctt cgtgatcgtc atcttcatgg ccgtgcactt ctggcgggtc
      661 cgtaaagacg gcggcatcag tgggcctctg taggagcgac tgagactcat ggtcgagatc
      721 cccgaacacc tccgtaagcg ggccgaagag gcccgagcca aggccgaggc cgctcgtggc
      781 ggcggcgacg gtggcaccga cgcgggcgat gcaggtggcg ccgacagcgg cataccgtcc
      841 gagctgctcg agcggtcgaa ggcggcgcgg gcccgggccg cgggtggcga cgccgacacg
      901 gcaccggccg cgtccggggg gggcacggcc gtggccacgg cgccggccgc cgccacgccc
      961 acaggccctg gtggccacac ccagcgcctg ctgaccgtgg tcaaggccgg ctccatccag
     1021 gacgtgaagg ccacgcccgt ggacaaggtc cacgtctggc cccacctgct gctgatcgag
     1081 ttcgtggccg ccctcgggat caccgccttc ctgctcttct tctcgatctt cgtcaacgcc
     1141 ccgctgctgg agctggccaa ccccaaccgg acgccgaacc cctccaaagc gccgtggtac
     1201 ttcctcggcc tccaggagct gctgacgatg ttccacccca tgatcgccgg ggtgaccatc
     1261 ccgaccatcg gcttgatcgg ctacgcgatg gtcccgtaca tcgacaagaa cccgagcacc
     1321 aagcccgagg accgcaagtt cgccatctcc ctgttcaccg tgcagctcat gttctgggcc
     1381 gtgctggtga tcatcgggtc cttcttccga ggccccggcc agaacttcgt ctttccctgg
     1441 aacgaaggga tcttcttcga cctgtaggtc acgcccatga gcaccggaac gatcctcctc
     1501 atcgtcatcc ctctgctggt cgtgctggcg ggcatcctcc tggccggcgc ggcgcgccgc
     1561 cgggagaccg gcgacgccgt cggtggcctc agccgcgaga cccgcaagcg ggaccagggc
     1621 gtcgccctgg acgccgacct cgagcccacg ggccgggacg tcgagcgtga ggccaagctg
     1681 gcccagtccg ggaagctggt cccggtcgac cccgcccccc tcgagcccta caccccgccc
     1741 gacgccgaga ccctcggggt cacgcggcgc cagttcctca accgggccat cctcggcttc
     1801 accggcttct cgttggctgc cttcggcggg gccgtcctgg cgttcatctg gcccaacgcc
     1861 cagggcgggt tcggctccaa gatccggatc ggcaacatcg acgacgtgaa ggccagcatc
     1921 gtcgaaggca acggcttctt ctacgtgccc gaggggcgca tgtggatcac cgagtacccc
     1981 agcgacgccc tggagaaggc cgcgcaggtc tactcacccg ccgagctgac gggcatggag
     2041 gccggcgtga acacgctgtt ccagacctgc ccccacctgg gctgtcgggt gccgaactgt
     2101 gacagctcgc agtggttcga gtgcccgtgc cacggctccc ggtacaaccg ggtgggcgag
     2161 aagcggggcg ggccggcccc ccggggcatg gaccgcttcc cgtcgacggt ctcgggcggt
     2221 gtgctcgaag tcgacaccgg catccgcatc cagggcccgc ccatcggcac caacaccacc
     2281 ggccaggagc cggagggccc cagctgcctc gggggttcga cgcacgaatg agcgttctcg
     2341 cgggggccgt cctggcctcg accgtctcca gcgtgggcta cgtcatcgtc gtcctgacgc
     2401 tgatcgcgtt cggcgtcgcc gtcttcatca acatccggcg ggcccgggcc gagaccggct
     2461 cggagctgga gctggcgccc aaccgggtgc cgcaccaccc cgacgaggtc ctcgagggcc
     2521 cggtgctcaa ccggaacctg gccttcggcc tcggggccat cgtgatcatc tcgatcggcc
     2581 tgcccgtcta ctggttgggt gagcccgccc gcaacgaggg cgcccaggcc accttcgacc
     2641 gcgtggccgc ggaacggggc gccgagcagt tcgcggtgac cgccgagggc ggcctcaact
     2701 gcgcgggctg ccacggcggc atggacgccc ccggcggcgt ggccccctac acgatcaacg
     2761 acgccaacgg cgagttcgtg gccagcgtcg agtggcaggc ccccgccctc aacaccgtcc
     2821 tcctgcgctt cgacgaagaa gagctctacg acatcctcgt ctacggccgt cccttctcgc
     2881 ccatgccggc gtggggcatc gagggcggtg gcgccctcaa cgaccagcag atcgggaacc
     2941 tgatcgccta cctcaagacg atccagataa cgcccgagga ggcccaggcg gcggcgaccg
     3001 acgccctggc cgacgccatg gcgagcggcc agtacgagac cgaaggcgag gccctgttca
     3061 acctcggcct ctacgacggc ttcgccgggg gcgcctactc ctgcgggcgc tgccacaccc
     3121 agggctggtc ctacggcgag cccgagtccg acggcgacgg ggcgctcggc cccaacctga
     3181 ccgccgttcg gagccagttc ccgggcgggg ccgcgggctt ccaggagcag gtcgacttcg
     3241 tgaccgaggg ctcggtcaag ggggtcctct acggcgccca cggccagggc acggggcgga
     3301 tgccgggctt cgacgacatg ctgaccgacg agcagatcca ggccatcgtg gagtacgaga
     3361 ggaacctcgg cgatgactga ttcgatcgtg ggcgtcctgc tggcggggat ctcctatgac
     3421 cccgagatcc gtggtgtgct ggtggtcacg gtggcggcgc tgatcctgat cggctccgtc
     3481 tacctcctgc tcagcaccaa cgtcggggcc cgcctcggct tccaggtggc catggccggc
     3541 ctcttcggct ggctgatgct gctcagcctc ttctggtgga tctacggcgt gggggccatc
     3601 gggaccctgc cctcgtggag ggtcgaggag atcaacgtcg gggacctgag ccaagcccaa
     3661 ctcgaggagg cccgtggcct cgtgcccgag aacctgccct cggccgaaga catcatcgcc
     3721 gagttccccg acgtggccga agcgatggcc gaggacccga cgcccaccct gagcgagatc
     3781 gccgccctgc ccgacctgcc ccccgaggtg gaagagcagc tggccggtct cgaggggggc
     3841 tgggacgtgc tgtcccaggc cgcgatcggt gacgcccagg ccgcggctga caccgccctg
     3901 atcagcgagg agaccgggct ctacacctcg gccagcgagt acgtgctggt ggacgggttc
     3961 acccgcgggg gcaaggagca gccggagagc gacagcctct gggaccgggt gtcgacccgg
     4021 gtcgccaacg ccttcacggt cctgaaccca ccgaagtaca cggtgatcca ggtccagacc
     4081 tcggagtcca ccgcggtgcc ggagggcaac gcccccctgt cgcccacccc ggacgaggac
     4141 cagcccgtcg tgtcggtgat catggtgcgg gacctcggga cgcggcggct gccgcctgcg
     4201 atgctgacga tcttgttcgg ca
//
LOCUS       DASTFV010000108         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_362412, whole genome shotgun sequence.
ACCESSION   DASTFV010000108 DASTFV010000000
VERSION     DASTFV010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362412"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..260)
                     /locus_tag="VFP02_01985"
     tRNA            complement(165..260)
                     /locus_tag="VFP02_01985"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(223..225),aa:Sec,seq:tca)
     gene            complement(379..1254)
                     /locus_tag="VFP02_01990"
     CDS             complement(379..1254)
                     /locus_tag="VFP02_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HET9541822.1"
                     /translation="MEAAFFDLDKTVIARASMVAFGKPLYRAGLLSRWLLLRALYGQL
                     VYLYLGADEARMAKMREAALRVTAGWSQDHIRSLVRETLEEVIEPIIYEEALDLIRFH
                     QAEGRKVFIVSASPQEIVDPLAQYLGVDDVIATRPQVDEHGRYSGGVDFYSYGPSKVI
                     AMEEAAERDGIDLAASYAYSDSITDEPMLSAVGHPVVVNPDRGLARIAAERDWEVRVF
                     QRPVKMRDRVSMPVPGRTAAISGGLAAIVAGVVVFWLLRREPEPSGWEQFGRSMRAWT
                     RRTARPKGRTALRRR"
     gene            1398..2048
                     /locus_tag="VFP02_01995"
     CDS             1398..2048
                     /locus_tag="VFP02_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase operon protein YhjQ/BcsQ"
                     /protein_id="HET9541823.1"
                     /translation="MHLSCWALKGGVGTTVVTAALGLTLTRAGHDVLLVDLAGDLPAA
                     LGLPEPDGPGLTDWLAAGDDVPADGLGRIEVPAVPGLAVLPRGRGELGPTDRVEALVA
                     ALDPRRSVAIDCGVLGACEPDRAVVAARCTHSLLVTRACYLALRRAADPPLRPSGVVL
                     VTEPGRALDRTDVEQVVGAPVRAEVALDPAVARAVDAGLLASRLPRGLERALRRAA"
     gene            2038..>2232
                     /locus_tag="VFP02_02000"
     CDS             2038..>2232
                     /locus_tag="VFP02_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541824.1"
                     /translation="MPPEGQELEARVHQRLLTEVPEGDGDRVVEVARAVGREAPLLTG
                     SERGGVADRVVARIRGLGALE"
BASE COUNT          293 a          801 c          823 g          315 t
ORIGIN      
        1 caagacctgg acgatgttgc accgggtcct caagggctac gactggtggc agcgccgcgt
       61 ccggcggacg acgccgaacg ggttcctgcc cccgcccgac tacgacccgg tcgcctggaa
      121 ggcgaagcag caggcgtgga gctgaccagc ccggcacgac ggcgtggcgg aggtggacgg
      181 gaatcgaacc cgccggacgg ggatcgcccg tcccacccgc tttgaaggcg ggggagccca
      241 ccaggtgctc tgacacctcc acccgacacg gtagggcggg accgacctag ggtggtccca
      301 tgaagaagct gctgttgctc ctggtcgtgg tggttctcgg cgtggtcgtt gcgaagaagc
      361 tccaggaagc ctgaacacct aacgccggcg tagggcggtc cgacccttgg gtcgggccgt
      421 tcgtcgcgtc caggcccgca tcgatcgccc gaactgctcc caaccggacg gctcgggctc
      481 gcgccgcagg agccagaaga ccaccacgcc ggccacgatg gcggccaggc ctccgctgat
      541 ggccgcggtg cgacccggga cgggcatgga cacccggtcc cgcatcttca ccggacgctg
      601 gaagacccgg acctcccagt cccgctcggc ggcgatgcgg gccaggcccc ggtcagggtt
      661 gaccacgacg gggtggccta cggcggacag catcggctcg tcggtgatgg agtccgagta
      721 cgcgtaggac gcggccaggt cgatgccgtc ccgctcggcc gcctcctcca tggcgatgac
      781 cttggacggc ccgtaggagt agaagtcgac gccaccggag tagcgaccgt gctcgtcgac
      841 ctggggccgg gtggcgatga cgtcgtcgac gccgaggtac tgggccaggg gatcgacgat
      901 ctcctggggc gaggcgctca cgatgaagac cttgcgcccc tcagcctggt ggaagcggat
      961 gaggtcgagg gcctcctcgt agatgatcgg ctcgatgacc tcttcgaggg tctcgcggac
     1021 cagcgagcgg atgtggtcct gggaccagcc ggccgtgacc cggagcgcgg cctcgcgcat
     1081 cttggccatc cgggcctcgt cggcgccgag gtagaggtag acgagctggc cgtacagggc
     1141 ccgcaggagc agccaacggg acaggaggcc ggcccggtag aggggcttgc cgaaggccac
     1201 catcgaggcg cgggcgatga cggtcttgtc caggtcgaag aacgcggcct ccatgcggcc
     1261 caccctagga acgcgaggac cccgttgttc gtgaacctcg tcacgcctaa cgtgggcttc
     1321 gtcgcgagtt caccggccgt gtgccgggct cgccccggcg cttcccccgc ctgtcacctc
     1381 tgctcggggg acgtcctgtg cacctttcct gctgggccct caagggcggc gtggggacca
     1441 ccgtggtcac cgccgccctc ggcctcaccc tcacccgcgc cggccacgac gtgctgctcg
     1501 tcgacctcgc cggcgacctg cctgccgcgc tcggcctgcc cgagcccgac ggtcccggcc
     1561 tcaccgactg gttggccgcg ggcgatgacg tccccgccga cgggctcggc cgcatcgagg
     1621 tgcctgccgt ccccggcctg gccgtcctgc ctcggggtcg cggcgagctc ggaccgacgg
     1681 accgggtcga ggccctggtg gccgccctcg atccccgccg ctcggtggcg atcgactgcg
     1741 gcgtcctcgg cgcctgcgag cccgaccggg cggtcgtcgc ggcccggtgc acgcactcgc
     1801 tgctggtcac ccgggcctgc tacctcgccc tgcgtcgggc ggccgacccg cccctgcgtc
     1861 cctccggggt cgtcctggtg accgagccgg gtcgggcctt ggaccgcacg gatgtcgaac
     1921 aggtcgtcgg tgcccccgtc cgggccgagg tggccctgga tcccgcggtc gcccgggcgg
     1981 tcgatgccgg gctgctggcc agccggctgc cccggggcct cgaacgcgct ctgcgccgtg
     2041 ccgcctgagg gccaggagct ggaggcccgg gtccaccagc gcctcctgac cgaggtcccg
     2101 gaaggagacg gggaccgggt ggtcgaggtg gcgcgggccg taggccgcga ggccccgctc
     2161 ttgaccggct cggagcgggg tggggtggcc gaccgggtgg tggcccgcat ccgaggcctg
     2221 ggagccctcg ag
//
LOCUS       DASTFV010000109         4414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_363749, whole genome shotgun sequence.
ACCESSION   DASTFV010000109 DASTFV010000000
VERSION     DASTFV010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363749"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFP02_02005"
     CDS             complement(<1..195)
                     /locus_tag="VFP02_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541825.1"
                     /translation="MSDGKMAIRADEVSKKFRLAHNRSEYLKEAIKRAERTEFTDFWA
                     LKNVSFEVEEGSMFGIIGHNG"
     gene            complement(195..1016)
                     /locus_tag="VFP02_02010"
     CDS             complement(195..1016)
                     /locus_tag="VFP02_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9541826.1"
                     /translation="MLTATDELWGYRALVQNLTNRQLKSQYKESLFGNLWSMINPAAT
                     IGVYSLVFGVLLKFGRNIPGIQYGDLQSFPLYLFCGIIMWSTFNRVAIGAMGTFSGTG
                     ALLKKVYFPPAAPIVAHGLAQIKQTMLEGLIAMIVLAALGYFSWSFVLWPLLVPPIVI
                     FSLGLGLVLTLANAYYKDVSYLMNILTMLLFYCTPILYSLERIGDAEVFGIKATTLLE
                     LNPLTHFVEASREMLYLGIAPSMVRWAAIWLASILSLAVGWAVFVRYSKDVSEVV"
     gene            complement(1030..1854)
                     /locus_tag="VFP02_02015"
     CDS             complement(1030..1854)
                     /locus_tag="VFP02_02015"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9541827.1"
                     /translation="MFVVYGMPRSGTTLVAQVLNAHTGLLVPDETDFMVPAAHAFRLV
                     EDPDLGRRLLGDLIASTDRFDATLGRWLTADRVRQVVADAPYRFDSIVASLYDALAEA
                     AAVTSAGDKSPNDLPQSEILGQAGFFTERIRAIHLVRDPRDVMVSMARLGWLNGIDVS
                     YARLWRNANLSLHQRLAAHPDRYRLVRYEDVVARPTEVFEAVADFLRVPFEPAMVSEE
                     ARFSQYPEHKGMAQHASTFRPIGGDSVGGYAELFDRSTLDEVEALAGDALSTFGYQ"
     gene            complement(1859..3484)
                     /locus_tag="VFP02_02020"
     CDS             complement(1859..3484)
                     /locus_tag="VFP02_02020"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9541828.1"
                     /translation="MFLVVGQPGSEARLVADTLEAHPDLVVAGRGELLVPLAFLLQRV
                     AEPVAARRLATDLIVADKGFADGIGRHLTAGEVAEVVFGSPLRLGPLLSQIFAAVARA
                     AGVRSAGTMLSVLANPVLNRVGLYEGDVRLLHVVRDARAVVAGSDAHGAAVDIARRWD
                     QANRLFRDRRGGDPATYALVRVEEFLHHPGRVVATVAPVLGIGATRHDLPRRTAPPAP
                     ALDRATQRAVTHTAREGLFVFGYVPPRYSPRRAAILGWRRGRALLERRRNSRNHRRSQ
                     AWALQVPPPWAPESEAETIALAACNICRWTGAAFTGQQHAESADCPQCGSIARERFHL
                     HGLAPDVDAGRLRLLETAPRLHGTYATAMARWCDYTELEPVPPGGPLGHLVGLEVMAD
                     ASADWIVSAHDLQAIPDPDAALAQFHRVLASGGVLLLQIPVLDATTTTLDAAVTAAPG
                     TARWAFGMDVLDRITGAGFAPELLVTDEFVDLAKGGRDAWSTVTSTGEVDLEGVLGAA
                     NSVTLTPIADRATARRRGWIPAVLFLTIRASRA"
     gene            complement(3488..4342)
                     /locus_tag="VFP02_02025"
     CDS             complement(3488..4342)
                     /locus_tag="VFP02_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541829.1"
                     /translation="MLVGERVIYLQLQKTGSTRITDLIQATVGAEERHKHDRIRRDDL
                     ADPDIAARRILGSIRSPWSYYVSLWAYGSRRAGGIYERTTATPRMDQARSLRGTLHKK
                     TIPLENRDRASRRRVKKWKTAYGNPEDREAFRRWIRFLNDPDYLVDVGEGYGKSAISR
                     HSGLLTYRYLVLFTLGVVPGEPHLGGHNPELETLAKVRDYADENLLCQDMIRMEHLAD
                     ELEASLVAAGYDLTAEQRELIHGQAEGRSENRTEHHPAAWYYDDATRDLVAERDRFVI
                     ERHGYEFG"
BASE COUNT          691 a         1437 c         1639 g          647 t
ORIGIN      
        1 gccgttgtgg ccgatgatgc cgaacatcga gccctcctcg acctcgaagg acacgttctt
       61 cagcgcccag aagtcggtga actcggtgcg ctcggcccgc ttgatggcct ccttcaggta
      121 ctcggaccgg ttgtgggcca accggaactt cttggacacc tcgtcggcgc ggatcgccat
      181 cttgccgtca ctcatcacac gacctcactg acgtctttgg agtatcggac gaacaccgcc
      241 cagcccacgg ccaggctcag gatcgaggcc agccagatgg cggcccaccg gaccatgctg
      301 ggcgcgatgc cgaggtagag catctcccga gaggcctcga cgaagtgcgt cagggggttg
      361 agctcgagca gggtggtggc cttgatcccg aagacctcgg cgtcaccgat gcgctccagg
      421 gagtacagga tgggcgtgca gtagaagagg agcatcgtga ggatgttcat caggtacgac
      481 acgtccttgt agtacgcgtt cgccagggtc aggaccagcc ccagacccag cgagaagatc
      541 acgatcggtg ggaccagcag cggccagagc acgaagctcc aggagaagta acccagggcc
      601 gcgagcacga tcatggcgat caggccctcg agcatcgtct gcttgatctg ggcgaggccg
      661 tgggccacga tcggggccgc gggcgggaag tacaccttct tcaacagcgc ccccgtcccg
      721 ctgaaggtcc ccatcgcgcc gatggcgacg cggttgaacg tcgaccacat gatgatcccg
      781 cagaagaggt acagcgggaa gctctgcaga tcgccgtact ggatgcccgg gatgttgcgg
      841 ccgaacttga ggagcacgcc gaagaccaac gagtagacgc cgatggtggc ggccgggttg
      901 atcatcgacc agaggttgcc gaagaggctc tccttgtact gggacttgag ttggcggttc
      961 gtcaggttct gaacgagagc tcggtagccc cagagctcgt cggtggcggt cagcatgtgc
     1021 tggaggtcct cactggtagc cgaaggtgga cagggcgtcg ccggcgaggg cctcgacctc
     1081 gtccagcgtc gatcggtcga acagttcggc gtagccaccg acgctgtccc caccgatggg
     1141 tcggaaggtg ctggcgtgct gggccatgcc cttgtgctcg gggtactgcg agaagcgggc
     1201 ctcctcgctc accatggccg gttcgaaggg cacccgcagg aagtcggcga cggcctcgaa
     1261 cacctcggtg gggcgggcca cgacgtcctc gtagcggacc agccggtagc ggtcggggtg
     1321 ggcggcgaga cgctggtgca ggctcaggtt cgcgttccgc cagaggcggg cgtagctcac
     1381 atcgattccg ttcagccatc ccagtcgtgc catggagacc atcacgtcgc gagggtcccg
     1441 aaccaggtgt atggcccgga tgcgctccgt gaagaacccg gcctgtccaa ggatctcgct
     1501 ctggggcagg tcattcggtg atttgtcgcc ggccgaggtg acggcagccg cctcggccag
     1561 agcgtcgtag aggctagcga cgatgctgtc gaagcggtac ggcgcgtcgg caacgacttg
     1621 ccgcacccgg tcggcggtga gccagcgccc gagggtggcg tcgaagcggt cggtcgacgc
     1681 gatgaggtca ccgaggagcc ggcgccccag gtcggggtcc tcgaccagtc ggaaggcgtg
     1741 agccgcggga accatgaagt cggtctcgtc cgggacgagg aggccggtgt gggcgttgag
     1801 gacctgggcc accagcgtcg tgcccgaccg gggcatcccg tagacgacga acacggcctc
     1861 aggccctcga agcccggatg gtgaggaaca ggacggccgg gatccagccc cgacgcctgg
     1921 ccgtcgcccg gtcggcgatc ggcgtcagcg tcaccgagtt ggcggcgccg agaacgcctt
     1981 cgaggtcgac ctcaccggta ctggtcacgg tggaccaggc atcccggccg cctttggcca
     2041 gatccacgaa ctcatcggtc accaggagct ccggcgcgaa gccggccccg gtgatccggt
     2101 cgaggacatc catgccgaac gcccaccgcg ccgtccccgg ggcggcggtg accgccgcat
     2161 ccagggtggt cgtggtcgcg tcgagcacgg ggatctgcaa caggagcaca cctccggacg
     2221 ccaggacccg gtggaactgg gccagcgcgg catcggggtc cgggatggcc tgcaggtcgt
     2281 gggccgagac gatccagtcg gccgaagcat cggccatgac ctcgaggccg acgagatggc
     2341 cgagggggcc gcccggcgga accggctcca gctcggtgta gtcgcaccac cgggccatcg
     2401 ccgtggcgta ggtgccgtgc aggcgagggg cggtctcgag gaggcggagg cgaccggcgt
     2461 cgacgtcggg ggcgaggccg tggaggtgga accgctcccg ggcgatgctg ccgcactggg
     2521 ggcagtcggc gctttcggcg tgttgctggc cggtgaaggc ggccccggtc caccggcaga
     2581 tgttgcaggc ggccagggcg atggtctccg cctccgactc gggggcccag gggggtggga
     2641 cctggagggc ccacgcctgg ctgcggcgat ggttccgtga gttgcgccgc cgctcgagca
     2701 gggctcgacc ccggcgccag ccgaggatgg ccgcccgccg cggcgaatac cggggcggca
     2761 cgtagccgaa gacgaacagg ccttcccggg cggtgtgggt gaccgcccgc tgggtggccc
     2821 ggtccagggc cggcgcgggg ggagccgtgc gccgaggaag gtcgtggcgg gtggcgccga
     2881 tcccgagcac gggggccacg gtcgccacca ctcgaccggg atggtgcagg aactcctcga
     2941 ctcggaccag ggcgtaggtg gcggggtcac cgccccggcg atcgcggaac aggcggttgg
     3001 cctgatccca gcgccgagcg atgtcgacgg ccgcgccgtg agcgtccgag ccggcgacga
     3061 cggcgcgagc gtctctgacc acatggagga gccggacgtc gccctcgtac aagccgaccc
     3121 ggttgagcac cgggttggcc agcacggaga gcatggtgcc cgcggatcgc accccggccg
     3181 cccgggcgac cgcggcgaag atctggctca ggagcgggcc gaggcgcagc ggcgagccga
     3241 acacgacctc ggccacctcg cccgcggtca ggtgccggcc gatcccgtcg gcaaagccct
     3301 tgtcggccac gatcaggtcg gtggccagcc gccgggcggc gacgggctcg gcgacgcgct
     3361 gcaggaggaa ggcgagcggc accaggagct ctccccgtcc ggctacgacc agatcggggt
     3421 gggcctcgag ggtgtcggcg acgaggcgcg cctcagaacc gggttggccg acgacgagga
     3481 acacggttca gccgaactcg tagccgtggc gctcgatgac gaagcggtcc cgctcggcca
     3541 cgaggtcccg ggtggcgtcg tcgtagtacc aggccgccgg atggtgctcg gtgcggttct
     3601 cgctgcggcc ctcggcctgg ccgtggatca actcgcgctg ctcggcggtg aggtcgtagc
     3661 cggccgccac cagggaggcc tcgagctcgt cggccaggtg ctccatgcgg atcatgtcct
     3721 ggcagagcag gttctcgtcg gcgtagtccc gcaccttggc caacgtctcg agctcggggt
     3781 tgtggccgcc cagatggggc tcaccgggca cgacaccgag cgtgaagagc accaggtacc
     3841 ggtacgtcag cagccccgag tgccgggaga tggcgctctt gccgtacccc tcgccgacgt
     3901 cgacgaggta gtccgggtcg ttcaggaacc ggatccagcg ccggaacgcc tcgcggtcct
     3961 cggggttgcc gtatgcggtc ttccacttct tcacccgccg ccgggacgcc cgatcgcggt
     4021 tctcgagggg aatcgtcttc ttgtgcagcg tcccccgcag cgaccgggcc tggtccatgc
     4081 ggggcgtggc cgtcgtccgt tcgtagatgc cgccggctcg ccgactcccg taggcccaca
     4141 aggagacgta gtacgaccac gggctgcgga tcgaacccag gattcggcgg gcggcgatgt
     4201 cggggtcggc caggtcgtct cgccggatgc ggtcgtgctt gtgacgctcc tcggccccga
     4261 cggtggcctg gatcaggtcg gtgatccggg tgctgcccgt cttctggagc tggaggtaga
     4321 tgacgcgttc gccgacgagc atcgccgcgt caggttaggg ccgcccccgc gccgcgacgc
     4381 ggtcctcgcg gggcgaccgg ccgtcaggcg ccga
//
LOCUS       DASTFV010000110         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_368545, whole genome shotgun sequence.
ACCESSION   DASTFV010000110 DASTFV010000000
VERSION     DASTFV010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368545"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VFP02_02030"
     CDS             <1..810
                     /locus_tag="VFP02_02030"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9541830.1"
                     /translation="DTYGLDSAHDYDPVWARCRDLKVAVAFHSGSIGWGSRVSPSNYM
                     LNHIGHLAEGQHALAKSLFLGGVTRRFPELRFAFLEGGVAWAAALYADIVGHWEKRNR
                     NAMAHLDPSGIDRAVLLELVGAHAPNVDLDPALRTLPRRTEDPDHLDEFAACGIEVAE
                     DIAELFATPFFFGCEADDPMVRMAFDTAANPFGSRLKAVFGSDIAHWDVPDMADVLCE
                     AHELVEYGLIDDDAFRDFVFTNPVRFFTDVNPDFFEGTVVADAVRDLNGAP"
     gene            807..2507
                     /locus_tag="VFP02_02035"
     CDS             807..2507
                     /locus_tag="VFP02_02035"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9541831.1"
                     /translation="MSHDLVVRGGVVVDGTGAPGRVADVAVRDGRIVAIGTVDDDGVE
                     TIDATGRVVTPGFIDLHTHYDAQLLWDPAATPSPLHGVTTVLGGNCGFTIAPLAEGDA
                     DYIRQMMAVVEGMPLASLEAGPAWDWLGFDEWLDRLDGAIAVNAGFLVGHSTIRRAVM
                     GDAAVTDEADADQVEAMATEVHRAIEAGALGFSSSWGEAHRDGDGHLVPSLAAHRDEL
                     VALAAAAGAHDGTTLEFIPCVGEIPDDRMALMADMAAAAGRPLNWNLLGSLSPVEIYQ
                     QQLTASDVAAERGATVVALALPDVMRLRMGPVLDGLAAFAEVAALPIDQRRRAVADPD
                     SRARLRDEAERAGRGPLAALSDGSLVEIAESDHWGGRTLAEVAAATGVDTVDVLIDVV
                     LVDGLALTMVLPTLVPSLGATDEGWAARVAVWQDPRVRLGGSDAGAHADMMCHANYPT
                     VVLGEVVRDRGLLSLEEAVRMLTDEPARLYGLKDRGRLAVGAHADLVVFDPETVGSEP
                     ARARHDLPAHGLRLYAEARGIDHVVVGGTEIVRHGVVTGACPGTVLRSGVDTETTAPS
                     "
BASE COUNT          337 a         1019 c          880 g          366 t
ORIGIN      
        1 gacacctacg gcctggacag cgcccacgac tacgaccccg tgtgggcccg ctgccgggac
       61 ctgaaggtgg cggtggcctt ccactccggc tcgatcgggt ggggcagtcg cgtctcgccg
      121 tccaactaca tgctcaacca catcggccac ctggccgagg gccagcacgc gttggccaaa
      181 tccctcttcc tcggcggggt gacccggcgg ttccccgagc tgcgcttcgc cttcctggag
      241 ggaggggtgg cctgggcggc ggctctctac gccgacatcg tcgggcactg ggagaagcgc
      301 aaccggaacg ccatggccca cctcgacccg tccggcatcg accgggccgt cctgctcgag
      361 ctggtcgggg cccacgcccc caacgtcgac ctcgaccccg ccctccgtac cctcccccgc
      421 cgcacggagg accccgacca tctcgacgag ttcgcggcct gtggcatcga ggtcgccgag
      481 gacatcgccg agctcttcgc caccccgttc ttcttcggct gcgaagctga cgacccgatg
      541 gtgcgcatgg cgttcgacac cgcagccaat ccgttcggct cgcgcctcaa ggccgtgttc
      601 gggtccgaca tcgcccactg ggatgtgccc gacatggccg atgtgctctg cgaagcccac
      661 gagctggtcg agtacggcct catcgacgac gacgccttcc gggacttcgt cttcaccaac
      721 ccggtgcgct tcttcaccga cgtcaacccg gacttcttcg agggcaccgt cgtggccgac
      781 gccgtgcgcg acctgaacgg cgccccgtga gccacgacct ggtcgtccgg ggcggggtgg
      841 tcgtcgacgg caccggtgcc cccggccgcg tggccgacgt cgccgtgcgg gacggccgca
      901 tcgtcgccat cggcaccgtt gacgacgacg gcgtcgagac catcgacgcc accggtcggg
      961 tcgtgacccc tggtttcatc gacctccaca cccactacga cgcccagctc ctgtgggacc
     1021 ccgcggcgac gccgtccccg ctgcacggcg tgaccacggt cctcgggggc aactgcggct
     1081 tcaccatcgc ccccctggcc gaaggggacg ccgactacat ccgccagatg atggccgtgg
     1141 tcgaggggat gcccctggcc tccctggaag cgggcccggc ctgggactgg ctgggcttcg
     1201 acgagtggct ggaccgcctc gacggcgcca tcgccgtcaa cgccggcttc ctggtcggcc
     1261 actccaccat ccgccgcgcc gtcatgggtg acgccgccgt caccgacgag gccgatgccg
     1321 accaggtcga ggccatggca accgaggtcc accgggcgat cgaggccggt gccctcggct
     1381 tctcctcgtc ctggggggag gcccaccggg acggggacgg ccacctcgtg ccgtccctgg
     1441 ccgcccaccg ggacgaactg gtcgcgctcg ccgccgccgc cggggcccac gacggcacca
     1501 ccctcgagtt catcccctgc gtcggcgaga tccccgacga ccggatggcc ctcatggccg
     1561 acatggccgc cgccgccggt cgccccctga actggaacct gctcggcagc ctgtcccccg
     1621 tcgagatcta ccaacagcaa ctgaccgcgt ccgacgtcgc cgctgagcgg ggcgccaccg
     1681 tggttgccct cgccctcccc gacgtgatgc gcctgcgcat gggcccagtt ctggacggcc
     1741 tcgccgcgtt cgccgaggtc gccgccctgc cgatcgacca gcggcgccgg gcggtggccg
     1801 accccgacag ccgggcccgc ctgcgggacg aggcggaacg ggctggccgt ggcccgctgg
     1861 ctgcgctgag cgacggctcg ctcgtcgaga tcgccgagtc cgatcactgg ggtggccgca
     1921 cgctggccga ggtggccgcc gccaccggcg tggacaccgt cgacgtgctc atcgacgtcg
     1981 tcctcgtgga cggcctggcc ctgacgatgg tcctgcccac gctcgtgcct tcgttggggg
     2041 ccaccgacga gggctgggcc gcccgggtcg cggtctggca ggacccccgg gtccgcctcg
     2101 gcggttcgga cgccggcgcc cacgccgaca tgatgtgcca cgccaactac cccaccgtcg
     2161 tcctcggcga ggtcgtgcgg gaccggggcc tgctgagcct ggaggaggcc gtgcgcatgc
     2221 tgaccgacga gccggcccgg ctctacgggc tgaaggaccg gggccgcctc gccgtcgggg
     2281 cccacgccga cctggtcgtg ttcgaccccg aaacggtcgg ctcggagccg gcccgggccc
     2341 gccacgacct cccggcccac ggcctccgcc tctacgcaga ggcccggggg atcgaccacg
     2401 tcgtggtcgg cgggaccgag atcgtccgcc acggcgtggt gaccggcgcc tgcccgggca
     2461 ccgtcctgcg cagcggcgtc gacaccgaga caacggcccc gtcatgagcg ccccggaccg
     2521 ggtcgccctc gtcaccgggg ccagtcgcgg catcggcaag gcctgcgccg tcgagctggc
     2581 caaggtcggc tacgacgtcg cc
//
LOCUS       DASTFV010000111         3171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_375179, whole genome shotgun sequence.
ACCESSION   DASTFV010000111 DASTFV010000000
VERSION     DASTFV010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375179"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3133)
                     /gene="carB"
                     /locus_tag="VFP02_02040"
     CDS             complement(<1..3133)
                     /gene="carB"
                     /locus_tag="VFP02_02040"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET9541832.1"
                     /translation="MLREEGYRVILANSNPATIMTDPDFADRTYIEPLTPAVLAAIID
                     RERPDAVLPTLGGQTGLNIAMALYGEGRIGVPGKPEMIGADATAIATAEDRERFKEAM
                     VEIGLDVPRSGIAHNLAEARAVADEIGLPVIIRPAYILGGRGTGMAATPEEFAKAAKH
                     GLESSPISEILIEQSIAGWKEFELEVMRDRADNCVVICSIENVDPMGVHTGDSITVAP
                     AQTLSDVEYQRMRDAAFACLRRVGVETGGSNVQFALDPTNGEMVVIEMNPRVSRSSAL
                     ASKATGFPIAKIAARLAVGYTLDEIPNDITEKTPACFEPTIDYVVTKVPRWAFEKFPG
                     QPAVLGTQMQSVGEAMAIGRTFPESLQKGLRSLEHGRHGLNCDPGETALDDLSDDELV
                     ARASIGTPDRPFELEAALRRGISIDDVYASTKVDPWFLDQISLIVEERAHLAETGFTA
                     MARADWRRAKRLGFADAQLAWLWGIGADEVRAARLAAGVRATFKTVDTCGAEFDAHTP
                     YHYSTYEDEDEVRPGEKPKVVILGSGPNRIGQGIEFDYCCVHASFALRDAGFETVMVN
                     CNPETVSTDYDTSDRLYFEPLTHEDVLNVLEAEADSGEIVGVIVALGGQTPLKLAGVL
                     PEGMVLGTSAAAIDLAEDRERWNALCARLEIPQPAGGTAVTVDEALAVVERVGYPVLM
                     RPSYVLGGRAMSIVYDDDGLRSAMAELAGFGSLGKEGGLSAERPVLVDRFLEDAVEVD
                     VDALRDHTGDCLIGGVMEHVEEAGVHSGDSACIIPPPSLSDETLAVIETHTRAIAEAL
                     DVRGLINVQYAVKANQVFVIEANPRASRTVPFVAKATGVPLVKLAARVMVGATLVELR
                     AEGLLVDKVVGDHVAVKEAVLPFDRFPDVDTVLGPEMRSTGEVMGIDLTVGLAFAKSQ
                     IAAGDRLPESGTVFLSLADRDKPTAEAAARLFRELGFAIAATAGTARFLEERGIEVAT
                     VVAKLGEETGHDAVELIQSGKVDLVVNSPRGRGPRADGAHIRRAAASANIPVVTTAAA
                     GLAAARGMADWSRH"
BASE COUNT          431 a         1097 c         1164 g          479 t
ORIGIN      
        1 cgtgacggga ccagtcggcc atgccccggg ccgcggcgag accggcggcg gccgtggtga
       61 ccaccgggat gttggccgat gccgcggccc gacggatgtg ggcgccgtcg gccctcgggc
      121 cccggccccg ggggctgttg acgaccaggt cgaccttgcc cgactggatc agctcgacgg
      181 cgtcgtgacc cgtctcctcg cccaacttgg cgaccaccgt cgccacctcg atcccccgct
      241 cctccaggaa gcgggcggtg cctgcggtgg cggcgatggc gaaacccagc tcgcggaaga
      301 gccgcgccgc ggcttcggcc gtgggcttgt cccggtcggc cagggagagg aagaccgtgc
      361 ccgactcggg cagccggtcg cccgccgcga tctggctctt ggcgaaggcc aggcccaccg
      421 tcaggtcgat gcccatgacc tcgcccgtgg agcgcatctc cgggccgagg acggtgtcga
      481 cgtcggggaa gcggtcgaag ggcagcaccg cctccttcac cgcgacgtgg tcgcccacga
      541 ccttgtcgac caggaggcct tcggcccgga gctccacgag ggtggcgccg accatcaccc
      601 gggccgccag cttcaccagc gggacgccgg tggccttggc cacgaacggc accgtgcggg
      661 aggcccgggg gttggcctcg atcacgaaga cctggttggc cttgaccgcg tactggacgt
      721 tgatgagccc ccggacgtcg agggcttcgg cgatggcccg ggtgtgggtc tcgatgacgg
      781 ccagggtctc gtcggacagc gacggcggcg ggatgatgca ggcgctgtcc ccggaatgga
      841 cccccgcctc ctcgacgtgc tccatgaccc cgccgatcag gcagtcgccg gtgtggtccc
      901 gcagggcgtc gacatcgacc tcgaccgcgt cttccaggaa gcggtcgacc aggaccggac
      961 gttcggccga gagcccgcct tccttgccca gcgagccgaa gccggcgagc tcggccatgg
     1021 ccgaccgcag gccgtcgtcg tcgtagacga tggacatggc acggcccccg aggacgtagg
     1081 agggccgcat cagcaccggg tagccgaccc gctcgacgac ggccagggcc tcgtccaccg
     1141 tgaccgcggt gccacccgcg ggctggggga tctccaggcg ggcgcagagg gcgttccagc
     1201 gctcgcggtc ctcggccagg tcgatggcgg cggcgctggt gccgaggacc atgccctcgg
     1261 gcaggacgcc ggccagcttg aggggggtct ggccgcccag ggccacgatg accccgacga
     1321 tctcccccga gtcggcctcc gcctcgagca cgttgagcac gtcctcgtgg gtcaggggct
     1381 cgaagtagag gcggtcgctg gtgtcgtagt cggtcgacac cgtctccggg ttgcagttga
     1441 ccatgaccgt ctcgaagccc gcgtcgcgga gggcgaagct ggcgtgcacg cagcagtagt
     1501 cgaactcgat gccctgcccg atccggttgg gcccggagcc gaggatgacg accttgggct
     1561 tctcccccgg ccggacctcg tcctcgtcct cgtaggtcga gtagtggtac ggcgtgtggg
     1621 cgtcgaactc ggccccgcag gtgtcgaccg tcttgaacgt cgcccggacg ccggccgcca
     1681 ggcgggcggc tcggacctcg tcggccccga tcccccacag ccaggccaac tgggcgtcgg
     1741 cgaaccccag ccgcttggcc cgacgccagt cggcccgggc catggccgtg aagccggtct
     1801 cggccaggtg ggcccgctcc tcgacgatca ggctgatctg gtccaggaac caggggtcga
     1861 ccttggtcga ggcgtagacg tcgtcgatgg agatcccccg gcggagcgcg gcctccaact
     1921 cgaagggccg gtcgggggtg ccgatcgagg cccgggccac cagctcgtcg tcgctcaggt
     1981 cgtccagcgc ggtctcgccc gggtcacagt tcagaccatg acggccgtgt tcgagggagc
     2041 gcaggccctt ctgcagtgac tcggggaacg tccggccgat ggccatggcc tcgcccacgg
     2101 actgcatctg ggtgccgagg acggcgggct ggcccgggaa cttctcgaag gcccagcggg
     2161 gcaccttggt gacgacgtag tcgatggtcg gctcgaagca ggccggggtc ttctcggtga
     2221 tgtcgttggg gatctcgtcc agcgtgtagc ccacggccag gcgggcggcg atcttggcga
     2281 tcgggaagcc ggtcgccttg gaggccaggg ccgacgagcg gctgacccgc gggttcatct
     2341 cgatgacgac catctcgccg ttcgtcgggt cgagggcgaa ctggacgttg gagcccccgg
     2401 tctccactcc gacccggcgc aggcaggcga aggcggcgtc ccgcatgcgc tggtactcga
     2461 cgtcggacaa ggtctgggcc ggggccaccg tgatcgagtc accggtgtgg acgcccatgg
     2521 ggtccacgtt ctcgatggag cagatgacga cgcagttgtc ggcccggtcc cgcatgacct
     2581 cgagctcgaa ctccttccac ccggcgatgg actgctcgat gaggatctcg gatatggggc
     2641 tggactcgag gccgtgcttg gccgctttgg cgaactcctc gggcgtcgcc gccatgcccg
     2701 tcccccggcc ccccaggatg taggcggggc ggatgatgac gggcaggccg atctcgtcgg
     2761 cgaccgcccg ggcctcggcg aggttgtggg cgatcccgct gcgcggcacg tcgaggccga
     2821 tctcgaccat cgcctccttg aaccgctcgc ggtcctcggc ggtggcgatg gccgtggcgt
     2881 cggccccgat catctctggt ttgccgggca cgccgatgcg accctcgccg tacagggcca
     2941 tggcgatgtt caggccggtc tggccgccga gcgtcggcag cacggcgtcg ggacgctcgc
     3001 ggtcgatgat cgccgccagc accgccggcg tcagcggctc gatgtaggtg cggtcggcga
     3061 agtccgggtc ggtcatgatc gtcgccgggt tcgagttggc gaggatgacg cggtagccct
     3121 cctcgcgcag cacgcggcac gcctgcgtgc cggagtagtc gaactcgcac g
//
LOCUS       DASTFV010000112         2647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_382325, whole genome shotgun sequence.
ACCESSION   DASTFV010000112 DASTFV010000000
VERSION     DASTFV010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382325"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFP02_02045"
     CDS             <1..201
                     /locus_tag="VFP02_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET9541833.1"
                     /translation="TAWAGAGKDYADALPRTVSGMLRSRANTIEGGTTEVNKNILGER
                     VLGLPREPDPWRDAPWGEVPRS"
     gene            198..1004
                     /locus_tag="VFP02_02050"
     CDS             198..1004
                     /locus_tag="VFP02_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET9541834.1"
                     /translation="MIAVGTEPYETLVVERDGHVGWLIFDRPHRGNAMDAVMLAELER
                     AWQDLDADPEVRVIVNTGNGTTFQTGLDVGQLAEDPGALREQSRRTKGAELKLTAWHN
                     GVRKPVIAAVNGVCAGGGLHFVADADIVIVAEDATFLDPHVSIGQVTAYEVVALVRMA
                     PAEAIFRMALVGRHERMDARRAHQLGICSEVVAPDRLRPAATELAGKIARNSPAAMAA
                     TKRALWGALEAGLTDACRAGSQELVTMWGHPDQTEGPAAWADKREAQWST"
     gene            992..1783
                     /locus_tag="VFP02_02055"
     CDS             992..1783
                     /locus_tag="VFP02_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET9541835.1"
                     /translation="MEHLIIEKHGSVGWLINDRPDQLNAMNSAMRDEFADAWLELDRD
                     PDVRVIVHTGNGRAFQTGVDVSEIAGDGVGFERYRESVENWDLHFTAWQQGVRKPVIT
                     AVNGICAGGGFHWVAEADIVIAASDAQFFDPHVSIGQVVAIEAIGLMRKMPAEAVMRM
                     ALVGRHERMSAQRAYELGMISEVVDPPDRLREAAQELAEKIAHNSPAAMAATKRALWG
                     SLELGLTDACQAGAQDLVSMWGHPDQTEGPAAFAERREPEWQPLD"
     gene            1825..>2647
                     /locus_tag="VFP02_02060"
     CDS             1825..>2647
                     /locus_tag="VFP02_02060"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9541836.1"
                     /translation="MLDDLIFVSVDDHVVEPPHLFEGRMPAAYADRAPKVIRTDAGDD
                     VWSFEGAMIPNIGLNAVAGRPKEEYGVDPTSYDEMRPGCWDIHERVKDMSAGGILAAM
                     CFPSFPSFSGRLFLTTEDKGLATAVVRAYNDWHIDEWCGTYPERHIPMAIPMLWDPEA
                     SAEEIRRVSAKGCHSLTFTENPATLGLPSFHSEHWDPMWRALVETGTIMNVHLGSSGQ
                     LAVTAPDAPMDVMITLQPMNICAAAADLVWSRVFKEYRDLKVALSEGGTGWIPYFL"
BASE COUNT          416 a          933 c          941 g          357 t
ORIGIN      
        1 acggcgtggg ccggcgcggg gaaggactac gccgacgcac tgccccggac cgtgtcgggc
       61 atgttgcgct cgcgggccaa caccatcgag ggtgggacca ccgaggtgaa caagaacatc
      121 ctgggggagc gggtcctggg cctgccccgc gagcccgacc cctggcggga cgccccctgg
      181 ggcgaggtcc ctcgctcatg atcgccgtgg gtaccgagcc ctacgagaca ctggtcgtcg
      241 agcgggacgg tcacgtcggc tggctgatct tcgatcgccc gcaccggggc aacgccatgg
      301 acgcggtgat gctggccgag ctggagcggg cgtggcagga cctggacgcc gaccccgagg
      361 tccgggtcat cgtgaacacc gggaacggga cgacgttcca gaccggcctc gacgtcgggc
      421 agctggccga ggaccccggc gccctgcggg agcagtcccg gcgcaccaag ggggccgagc
      481 tgaagctgac ggcgtggcac aacggcgtgc gcaagccggt gatcgccgcc gtcaacgggg
      541 tgtgtgcggg cggcggcctg cacttcgtcg ccgacgccga catcgtcatc gtggccgagg
      601 acgccacctt cctcgacccc cacgtctcga tcggccaggt caccgcctac gaagtcgtcg
      661 ccctggtccg gatggcaccg gccgaggcca tcttccgcat ggccctcgtc ggccgtcacg
      721 agcggatgga cgcccggcgg gcccaccaac tggggatctg ctcggaggtg gtggcccccg
      781 accggctccg gcccgcggcc accgagctgg ccgggaagat cgcccgcaac agcccggcgg
      841 cgatggccgc caccaagcga gccctgtggg gcgcgctgga ggcgggcctc accgacgcct
      901 gccgggccgg ctcccaggag ctggtgacga tgtggggcca ccccgaccag accgaagggc
      961 ccgccgcctg ggccgacaag cgggaggcgc agtggagcac ctgatcatcg agaagcacgg
     1021 gtccgtgggc tggctgatca acgaccggcc ggaccagctc aacgccatga acagcgccat
     1081 gcgcgacgag ttcgccgacg cctggctcga gctggaccgg gaccccgacg tgcgggtcat
     1141 cgtccacacg ggcaacggcc gagcgttcca gaccggggtc gacgtgtccg agatcgccgg
     1201 cgacggcgtc ggcttcgagc gctaccggga gtcggtggag aactgggacc tgcacttcac
     1261 ggcctggcag cagggggtgc gcaagccggt catcaccgcc gtcaacggga tctgtgccgg
     1321 aggtggcttc cactgggtgg ccgaggccga catcgtcatc gccgcctccg acgcccagtt
     1381 cttcgacccc cacgtgtcca tcggccaggt ggtggccatc gaggccatcg ggctgatgcg
     1441 caagatgccg gcggaggcgg tcatgcgcat ggccctggtc ggccggcacg agcgcatgtc
     1501 ggcccagcgg gcctacgagc tgggcatgat cagcgaggtg gtggatccgc ccgatcgtct
     1561 gcgagaggcg gcccaggagc tggccgagaa gatcgcccac aacagcccgg cggcgatggc
     1621 ggccaccaag cgcgccctgt gggggtccct cgagctcggc ctgaccgacg cctgccaggc
     1681 gggggcccag gacctcgtgt cgatgtgggg ccaccccgat cagaccgagg gccccgccgc
     1741 cttcgccgag cgccgggagc cggagtggca gcccctcgac tagattctga accgagattc
     1801 atcccccgga acgggagttc tgagatgctc gacgacctca tcttcgtgag cgtggacgac
     1861 cacgtggtcg agccgcccca cctcttcgag ggccggatgc cggcggccta cgccgaccgg
     1921 gcgcccaagg tgatccgcac ggacgcgggg gacgacgtct ggtccttcga aggggccatg
     1981 atccccaaca tcggcctcaa cgccgtcgcc ggtcggccca aggaggagta cggcgtcgac
     2041 cccacctcct acgacgagat gcgcccgggc tgctgggaca tccacgagcg ggtgaaggac
     2101 atgagcgccg gcgggatcct ggccgccatg tgcttcccgt cgttcccgtc gttctccggc
     2161 cgtctcttcc tgaccaccga ggacaagggc ctcgccaccg cggtcgtgcg ggcctacaac
     2221 gactggcaca tcgacgagtg gtgcggcacc tatcccgagc gccacatccc gatggccatt
     2281 cccatgctgt gggaccccga ggccagcgcg gaggagatcc ggcgggtgtc ggcgaaggga
     2341 tgccattcgc tgaccttcac cgagaacccg gcgacgctcg gcctgccgag cttccacagc
     2401 gagcactggg accccatgtg gcgggccctc gtggagaccg ggacgatcat gaacgtccac
     2461 ctcggctcgt ccggccagct ggcggtgacc gccccggacg ccccgatgga cgtgatgatc
     2521 acgctgcagc cgatgaacat ctgcgccgcg gccgccgacc tcgtctggtc ccgtgtcttc
     2581 aaggagtacc gcgacctgaa agtggcgctg tccgagggcg ggaccggctg gatcccctac
     2641 ttcctcg
//
LOCUS       DASTFV010000113         2351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_383646, whole genome shotgun sequence.
ACCESSION   DASTFV010000113 DASTFV010000000
VERSION     DASTFV010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383646"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFP02_02065"
     CDS             complement(<1..430)
                     /locus_tag="VFP02_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9541837.1"
                     /translation="MTDGAPNSGRLSGEVALVTGSTAGLGRVIALRLAAEGAGVVVTG
                     RDQERGEAVAYEVGGTFVAADLNAEAEATALVDAAARRFGSLTILVNNAVASVTSTPV
                     DEIATDDWDAALRVNVTAPMWLCRAAIPHFRAARHGSIVNV"
     gene            501..1409
                     /locus_tag="VFP02_02070"
     CDS             501..1409
                     /locus_tag="VFP02_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9541838.1"
                     /translation="MSGYLEGKSIAVTGAGRGIGRAVALACAAEGASVVVNDYGVAPD
                     GSEPTSEVADAVVEEITDAGGTAVAVADSVAEMAGGERIVATAVEQFGKIDGVVCPAG
                     ILRERMLFNMSEDEWDPVIATHLKGTFTVFRAAAARMKEQKSGSLIGFTSGAFAGSVA
                     QANYAAAKGGIVSLVRSAAAGMYRYGVTSNCIAPVAQTRLSAQVPMELQMGDAEDVAP
                     MVVYLLSDEARHITGQVYTVNGPTIAVWDQPHEVRSMDHEGGGRWTPAEIAAQIDEGV
                     GQERMGLIDRLDAMRAAAASGDKPNQ"
     gene            complement(1479..2183)
                     /locus_tag="VFP02_02075"
     CDS             complement(1479..2183)
                     /locus_tag="VFP02_02075"
                     /inference="COORDINATES: protein motif:HMM:NF022586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HET9541839.1"
                     /translation="MTGYRRRIELVTVSPEEVWGELEDDFHHFRVTVVHRDGKVVGID
                     GETPRHPWSTCPDALAPLQALVGAPVTADATAHGAHADARTTCTHWFDLAGLAIAQAG
                     AGRDHRRYDITVPDRGDGGRTTAHLARDGVDLLDWDVDARTVLGPEPYAGQPMGRGFL
                     AWANATLDPDTAEAAIALRRACRISLGRLMDLDDYERASEVGHAMADTCHTFSTDVME
                     TALRIKGSSVTLDWPR"
BASE COUNT          338 a          815 c          881 g          317 t
ORIGIN      
        1 agacgttcac gatcgatccg tggcgggcgg cccggaagtg cggaatggcg gcccggcaca
       61 accacatcgg cgcggtcacg ttcacccgca gggcggcgtc ccagtcgtcg gtggcgatct
      121 cgtcgaccgg cgtcgacgtc acggatgcca ccgcgttgtt gaccaggatc gtcagcgagc
      181 cgaagcgccg agcggcggca tcgacgagcg ccgtcgcctc cgcctcggcg ttcaggtcgg
      241 cggcgacgaa ggtcccgccc acctcgtagg cgaccgcttc gccccgctcc tggtcccgcc
      301 cggtcactac gacgcctgcc ccctcggcgg ccaggcggag ggcgatgacc cgacccagtc
      361 cggccgtgga gccggtcacc agggccacct ctccggagag ccgccccgag ttcggagccc
      421 cgtcggtcac gtgcgtaagg taaccagccg cacatgacgc aggcgtcagc ttggctggcc
      481 caccgacgag aggcacagag atgagcgggt acctggaggg caagtcgatc gccgtcaccg
      541 gggcgggtcg aggcatcggg cgggccgtgg ccctggcctg cgcggccgag ggtgccagcg
      601 tcgtggtcaa cgactacggg gtggctcccg acggctccga gccgaccagc gaggtggccg
      661 acgccgtcgt cgaggagatc accgacgccg gtggcaccgc cgtcgccgtg gccgactcgg
      721 tggccgaaat ggcaggcggg gagcggatcg tggccacggc cgtggagcag ttcggcaaga
      781 tcgacggcgt cgtgtgcccg gccgggatcc tgcgggagcg gatgctgttc aacatgtccg
      841 aggacgagtg ggacccggtc atcgccaccc acctcaaggg cacgttcacc gtcttccggg
      901 ccgccgccgc ccgcatgaag gagcagaagt cgggctcgct gatcggcttc acgtccggtg
      961 cgttcgccgg cagcgtggcc caggccaact acgccgcggc caagggcggc atcgtctccc
     1021 tggtccgctc ggccgccgcc gggatgtacc gctacggcgt cacgtccaac tgcatcgccc
     1081 ccgtcgccca gacccgcctg tcggcccagg tgccgatgga gctgcagatg ggtgacgccg
     1141 aggacgtcgc cccgatggtc gtctacctgt tgtcggacga ggcccgccac atcaccggtc
     1201 aggtgtacac cgtgaacggc ccgacgatcg ccgtctggga ccagccccac gaggtccgct
     1261 cgatggacca cgaggggggc gggcgctgga cgccggcgga gatcgcggcg cagatcgacg
     1321 agggagtggg tcaggagcgc atgggcctca tcgaccgtct cgacgccatg cgggccgccg
     1381 ccgcctccgg cgacaagccc aaccagtagg tcgcgctgcg cagcggagcg gagcagctcc
     1441 gaggaccgca gcttcggcgg cgccggactt ggcgagcgtc agcgaggcca gtcgagtgtg
     1501 acggacgagc ccttgatgcg gagggcggtc tccatgacgt cggtcgagaa ggtgtggcag
     1561 gtgtcggcca tggcgtggcc gacttcggag gcccgctcgt agtcgtccag gtccatcagg
     1621 cgaccgaggg agatgcggca ggcccggcgc agggcgatgg cggcctcggc cgtgtccggg
     1681 tccagggtgg cgttggccca ggccaggaag ccccgcccca tcggctgccc cgcgtacggc
     1741 tcgggaccga ggacggtccg tgcgtcgacg tcccagtcga ggaggtcgac cccgtcgcgg
     1801 gccaggtggg ccgtcgtccg cccaccgtca ccccggtcgg gcaccgtgat gtcgtagcgg
     1861 cggtggtccc gcccggcgcc ggcctgagcg atggccaggc cggcgaggtc gaaccaatgg
     1921 gtgcaggtcg tgcgggcgtc ggcgtgggcg ccgtgggccg tggcgtcggc cgtcaccggc
     1981 gcgccgacga gggcctggag tggcgcgagg gcgtccgggc aggtggacca gggatgacgt
     2041 ggggtctcgc cgtcgatgcc caccaccttt ccgtcccggt ggacgaccgt cacccggaag
     2101 tggtggaagt cgtcctccag ctccccccag acctcctccg gcgacaccgt gaccagctcg
     2161 atccgtcgcc ggtaccccgt cacgggaagc cgggcttgaa gatcatgagg aagaggatgg
     2221 tgaccaggag gaccttgagg accatgtcgg tcatcccgat cttccggccc agcgcctcga
     2281 gctggaccac ctggggcggc gggccctggg cgccgggggg tgggccctcg gtccgggaca
     2341 gctcctcggc c
//
LOCUS       DASTFV010000114         2881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_386056, whole genome shotgun sequence.
ACCESSION   DASTFV010000114 DASTFV010000000
VERSION     DASTFV010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386056"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VFP02_02080"
     CDS             <1..639
                     /locus_tag="VFP02_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541840.1"
                     /translation="GAGVTPAFVDEATERLGAVVKRAYGSTEAPTVATAHADDPTDRG
                     RDTDGRATGEVRFRLGDGDELLVSGPELCAGYDDPEATKAAFIDGWYRTGDRATIDDE
                     GWITITGRLKSVIIRGGENVSVAAVEATLEAHPSVRHAVVVGAPDDRLGERVVAVVTA
                     DEAFDLADCQRWFAEQGATKFTWPERVIVVDRIPTLPAGKPDRDAIRRLVTG"
     gene            complement(899..1957)
                     /locus_tag="VFP02_02085"
     CDS             complement(899..1957)
                     /locus_tag="VFP02_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9541841.1"
                     /translation="MDFELSDDQVALRDGARSLLDGRATPTQVRAVVDAEGGIDDGLW
                     SAMVEQGWTALAVPEDRGGLGLGIVELVVLLEEVGGHVAPAPVLQQAMALDALVTGDG
                     PADLIAPLVAGEAIGTVAFSPVTSDGSTVTGRPEPVIHAPSARVLIVRTADELYAVDL
                     EAAGLSPAAEPAMDRTRALGWVELDAAPAVRLGGADAVERYLDLGAVGHSAELLGASA
                     RVLDMAVEYAKERVQFGRPIGSFQAIKHRCADMLVDVEGMRSAVYHAAWALGAGDPDR
                     SVAASTAKTWCSDASRRVMASGLQVHGGIGFTWEHDLHLFVKRAQLDGVSFGDATWHR
                     DRLGRLLRAKLEAGQPLL"
     gene            1978..>2881
                     /locus_tag="VFP02_02090"
     CDS             1978..>2881
                     /locus_tag="VFP02_02090"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9541842.1"
                     /translation="MDLTPTREQEAFRAELRAWLAEHPPDVDLRAHHEDLAQEVVDLR
                     QWQARLAEGRWIGVTWPEEYGGRGAGALHHFIVQEELARARAPELVGRIGVNLVGPTL
                     LAHGTAEQKARWLPRILAADELWCQLFSEPGAGSDLAAVSTKAERVDGGWRLTGQKVW
                     TSYAQFADWGLCLARSDPDAPKNRGISALAVDMRAEGVEVRPLVQITGEAEFNEVFFD
                     GAFVPDDRLIGAEHDGWRVSSSTLTHERGTNPRQFVIHAQLVEELFRLAIESGRYDDP
                     RLQRKLAEAFVEVRLFQLHNWRSLS"
BASE COUNT          400 a         1083 c         1018 g          380 t
ORIGIN      
        1 ggcgccggcg tcaccccggc tttcgtggac gaggccacgg aacgcctcgg cgccgtggtg
       61 aaacgggcct acggctcgac cgaagccccc accgtggcga cggcccacgc cgacgacccg
      121 accgaccggg gccgcgacac cgatggccgg gccacgggcg aggtccggtt ccgactcggc
      181 gacggcgacg agctcctcgt cagcggcccc gagctgtgcg ccggctacga cgatcccgaa
      241 gccaccaagg ccgccttcat cgacggctgg tatcgcaccg gcgaccgggc cacgatcgac
      301 gacgagggct ggatcaccat caccggccgg ctgaagagcg tcatcatccg cgggggcgag
      361 aacgtctccg ttgccgccgt cgaggccacc ctcgaggccc acccgtcggt ccgccacgcc
      421 gtcgtcgtcg gcgcgcccga cgaccgcctc ggcgagcggg tcgtcgccgt ggtgacggcc
      481 gacgaggcct tcgacctcgc cgactgccag cggtggttcg ccgagcaggg cgccaccaag
      541 ttcacctggc ccgagcgcgt gatcgtggtc gaccgcatcc ccaccctccc cgcgggcaag
      601 cccgaccgcg acgcgatccg gcggctggtc accggctgag gctcttcgac gtcgacgtgg
      661 aggagcagac ggcgtcggag atggtgctcg ccgtgtcggt cggtggaggg actgatgctg
      721 cgtgtgcgac ggggcgatgg tcgtgctgtc gcacccgctg ggtgaccgtc gagtcatggt
      781 cgagggcgcg aacggacgta cgagatcgac gtgtggcggt acggggaggt ggcccgccgc
      841 cgacgaccag cctcagttcg ttcctgactc cctagtcgcc acgcctcagc tcgacggctc
      901 agagcagggg ttggcccgct tcgagcttgg cccggaggag gcgtccgagg cggtcgcggt
      961 gccaggtggc gtcgccgaag ctgacgccgt cgagctgggc ccgcttcacg aacaggtgca
     1021 ggtcgtgctc ccaggtgaag ccgatgccgc cgtggacctg gaggcccgag gccatgaccc
     1081 ggcgggaggc gtcggagcac cacgtcttgg ccgtggaggc ggcgacggag cggtcggggt
     1141 cgcccgcgcc cagcgcccag gcggcgtggt agacggcgga gcgcatgccc tcgacgtcga
     1201 ccagcatgtc ggcgcagcgg tgtttgatgg cctggaagct gccgatgggt cggccgaact
     1261 ggacccgttc cttggcgtac tccacggcca tgtccagcac ccgggcggac gctccgagca
     1321 gctcggcact gtgaccgacg gccccgaggt cgaggtagcg ctcgacggcg tcggccccgc
     1381 ccaggcggac ggcaggcgcg gcgtcgagct cgacccagcc cagcgcccgg gtccggtcca
     1441 tggccggctc cgccgcgggg gagagcccag ccgcctccag gtcgaccgcg tacagctcgt
     1501 ccgccgtgcg cacgatcagc acccgggctg acggggcgtg gatgaccggc tccgggcgcc
     1561 ccgtcacggt cgacccgtcg gatgtcaccg gcgagaaggc gacggtcccg atggcttccc
     1621 ccgccaccag cggggcgatc aggtcggccg ggccgtcacc tgtgaccagg gcgtccaggg
     1681 ccatcgcctg ctgcagcacg ggggccgggg cgacatggcc accgacctcc tccagcagca
     1741 ccaccagctc gacgatcccc agccccaggc cgccccgatc ctcgggcacg gccaacgccg
     1801 tccagccctg ctcgaccatg gccgaccaca gtccgtcgtc gattcctccc tccgcatcga
     1861 ccaccgctcg gacctgcgtc ggagtcgccc ggccgtcgag caggctgcgg gcgccgtcgc
     1921 gcagggcgac ctgatcgtcc gagagctcga aatccacgtg cgtaccattg catccccgtg
     1981 gacctgaccc cgacgcgcga gcaggaggcc ttccgggcgg agctccgtgc gtggttggcc
     2041 gagcacccgc ccgacgtcga cctgcgggcc caccacgagg acctggccca ggaggtcgtc
     2101 gacctgcgcc agtggcaggc ccgcctggcc gaggggcgct ggatcggggt gacctggccc
     2161 gaggagtacg gcggccgggg cgccggcgcc ctgcaccact tcatcgtcca ggaggagctg
     2221 gcccgcgccc gggcccccga gttggtcggc cgcatcggcg tcaacctggt cggccccacc
     2281 ctgctggccc acggcacagc cgagcagaag gcccgctggc tgccccggat cctggccgcc
     2341 gacgagctgt ggtgccagct cttctccgag cccggcgccg gctccgacct ggccgcggtg
     2401 tccacgaagg ccgagcgggt cgacgggggc tggcgcctga ccggccagaa ggtctggacc
     2461 agctacgccc agttcgccga ctggggcctc tgcctggccc gctccgaccc cgacgccccc
     2521 aagaaccggg gcatctccgc cctcgccgtg gacatgcggg ccgagggcgt cgaggtccga
     2581 ccgctcgtgc agatcaccgg cgaggcggag ttcaacgagg tcttcttcga cggcgccttc
     2641 gtccccgacg accgcctgat cggtgccgag cacgacggct ggcgggtgtc tagctctacc
     2701 ctcacccacg agcggggcac caacccccgc cagttcgtca tccacgccca gttggtcgag
     2761 gagctgttcc gtctcgccat cgagtccggc cgctacgacg acccccgcct gcagcggaag
     2821 ctggccgagg cattcgtcga ggtccgcctc ttccagctcc acaactggcg ctcgctctcc
     2881 c
//
LOCUS       DASTFV010000115         2750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_388433, whole genome shotgun sequence.
ACCESSION   DASTFV010000115 DASTFV010000000
VERSION     DASTFV010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388433"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /locus_tag="VFP02_02095"
     CDS             <1..1557
                     /locus_tag="VFP02_02095"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9541843.1"
                     /translation="EVALSVDEGDRAHFPVVVVGCGQSGLLAGIRLQEAGIPYTIVEK
                     NAGVGGTWFENTYPGCRVDVGNHFYCYSFEPSDHWSQYFARQPELQAYFDSVMRRHGV
                     AEHVRWETEVVRATWDDGAGTWSVRIRDHDGAEETLTARAVISAVGQLNRPNLPAIAG
                     RDDFAGPAFHSARWDHSVDLTGKRVALIGAGASGFQIAPAIADEVAHLTVFQRTAQWM
                     FPNPNYHETVGPGVQWALRHLPFYGRWYRFLLFWPACDGGLEAARIDPAYDDSDGRAV
                     SETNEFTRQMFTDWIVSQIGDDDPDLLAKVIPDYPATGKRTLQDNGSWLTTLTRPDVD
                     LVREGIDHIEADAVVTADGVRHDVDVIVFATGFQANRFLFPIEVVGRDGTVLAERWGE
                     RPSAYLGITVPGFPNLFCMYGPGTNLAHGGSLIFHSECQMRYITGCLDLLLSSGAKAM
                     EPRPEVHDAYYERTQQELKGLVWSHPSIRHSWFKNADGDIHVLSPWRLVDYWAWTKAP
                     DPADFDVTAA"
     gene            complement(1568..2314)
                     /locus_tag="VFP02_02100"
     CDS             complement(1568..2314)
                     /locus_tag="VFP02_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541844.1"
                     /translation="MTAAEPLIEVELPAPVEEVWAHLRDPGLIRRWFGWEYEGLEGEI
                     EFIFLGPPPEALAGMPEGAGFEVDDGAHTITWAYGGVDRDRFELRAEGDGCVLRVTRT
                     ISPESWDGVYDDVGEGWISFVQQLRFALTRHRGEERRTVFTSSAVTATDDVLAVLGVD
                     RVDGPWAGMLGPEPGRSGDLWFRSDHQLAVTVDQWNDGLLLVTWPRDSASVTLSVFGL
                     DGAAVVDLGERWNAWWVDHVGTPDPDSPYT"
     gene            complement(2311..>2750)
                     /locus_tag="VFP02_02105"
     CDS             complement(2311..>2750)
                     /locus_tag="VFP02_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET9541845.1"
                     /translation="AAGLALGADGVWCGSVWLTTEEAETPPSVKDKFLAAGSSDTVRS
                     RSMTGKPARMLRTAWTDEWERPDGPGPLPMPMQPILVAPALQRVARVADAAGSGGQQL
                     VTYFVGQVVGAMNTVKPTRRVVLEMVEEFIDAVERLDDLVQSE"
BASE COUNT          413 a         1020 c          932 g          385 t
ORIGIN      
        1 gaggtcgccc tcagcgtcga cgagggcgat cgggcccact tccccgtcgt ggtcgtcggt
       61 tgcgggcaat cggggctcct ggccgggatc cggctgcagg aggcgggcat cccgtacacg
      121 atcgtcgaga agaacgccgg ggtcgggggc acctggttcg agaacaccta ccccggctgc
      181 cgggtcgacg tcggcaacca cttctactgc tactccttcg agccgtcgga ccactggagc
      241 cagtacttcg cccggcagcc cgagctccag gcctacttcg actcggtgat gcggcgccac
      301 ggggtcgccg agcacgtgcg ctgggagacc gaggtggttc gggcgacgtg ggacgacgga
      361 gccggcacct ggtccgtgcg gatccgggac cacgacgggg ccgaggagac cctgaccgcc
      421 cgcgccgtca tctcggcggt gggacagctc aaccggccca acctccccgc catagccggc
      481 cgcgacgact tcgccggacc ggccttccac tccgcccgtt gggaccactc ggtcgacctc
      541 accgggaagc gggtggccct gatcggggcc ggcgccagcg ggttccagat cgcccccgcc
      601 atcgccgacg aggtggctca cctcaccgtc ttccaacgca cggcacagtg gatgttcccg
      661 aaccccaact accacgagac agtcgggccg ggcgtgcaat gggcgctgcg ccatctcccc
      721 ttctacgggc gctggtaccg gttcctgctc ttctggccgg cgtgcgacgg tggcctggaa
      781 gccgcccgca tcgaccccgc gtacgacgac tcggacgggc gggcggtcag cgagaccaac
      841 gagttcaccc gccagatgtt caccgactgg atcgtcagcc agatcggcga cgacgatccc
      901 gatctgctgg ccaaggtcat ccccgactac ccggccaccg gcaagcgcac gctgcaggac
      961 aacggcagct ggctgacgac cctcacccgg cccgacgtcg acctggtgcg cgaggggatc
     1021 gaccacatcg aggccgatgc cgtggtcacc gccgacgggg tgcgccacga cgtcgacgtc
     1081 atcgtgttcg ccaccgggtt ccaggccaac cgcttcctgt tccccatcga ggtggtgggc
     1141 cgtgacggca cggtcctggc cgagcggtgg ggcgagcggc cgtcggccta cctgggcatc
     1201 accgtccccg gcttccccaa cctgttctgc atgtacgggc ccgggacgaa cctggctcac
     1261 ggcggctcgc tgatcttcca cagcgagtgc cagatgcggt acatcaccgg ctgtctcgac
     1321 ctgctcctgt catcgggggc gaaggccatg gagcccaggc ccgaggtcca cgacgcgtac
     1381 tacgagcgca cccagcagga gctgaagggc ctcgtctggt cccacccttc gatccggcac
     1441 tcgtggttca agaacgcgga cggcgacatc cacgtcctca gcccctggcg cctcgtcgac
     1501 tactgggcct ggacgaaggc gcccgacccg gccgacttcg acgtgaccgc cgcttgagcc
     1561 cgcgacctca tgtgtacggc gagtccgggt cgggggtgcc gacgtggtcg acccaccagg
     1621 cgttccagcg ctcgccgagg tcgaccacgg cggcgccgtc gagaccgaag acggacaggg
     1681 tcaccgacgc gctgtcccgc ggccaggtca ccagaagcag gccgtcgttc cactggtcga
     1741 cggtcaccgc cagctggtgg tcggaccgga accacaggtc gcccgagcgc cccggttcag
     1801 gaccgagcat cccggcccag ggcccgtcga cccggtccac cccgagcacg gcgaggacgt
     1861 cgtcggtggc cgtgaccgcc gacgacgtga agacggtccg ccgctcctcg ccgcggtgcc
     1921 gggtcagggc gaagcggagc tgctggacga agctgatcca gccctcgccc acgtcgtcgt
     1981 agaccccgtc ccagctctcc gggctgatcg tgcgggtcac ccgcagcaca cagccgtcgc
     2041 cttcggcgcg gagctcgaac cggtcccggt cgacgccccc gtacgcccag gtgatggtgt
     2101 gggccccgtc gtcgacctcg aagccggcgc cctccggcat cccggccagc gcctccgggg
     2161 gcgggccgag gaagatgaac tcgatctcgc cctccaggcc ctcgtactcc cagccgaacc
     2221 agcgccggat gaggccgggg tcccgcagat gggcccacac ctcttcgacg ggcgcgggca
     2281 gctcgacctc gatcaggggc tcggcggcgg tcattcggac tggaccaggt cgtcgaggcg
     2341 ttcgacggcg tcgatgaact cctccaccat ctccaggacg acgcggcggg tgggtttgac
     2401 cgtgttcatg gccccgacga cctggcccac gaagtaggtc accagctgct ggccgccgct
     2461 gccggcggcg tccgcgaccc gggcgacccg ctgcagcgcg ggggccacga gaatcggctg
     2521 catgggcatg ggcagcgggc cggggccatc gggccgctcc cactcgtcgg tccaggccgt
     2581 gcgcagcatc cgcgcgggct tgcccgtcat cgagcgcgag cggaccgtgt cggaggaccc
     2641 ggccgccagg aacttgtcct tgaccgacgg tggggtctcg gcctcctcgg tggtcaacca
     2701 gaccgaaccg caccagaccc cgtcggcccc gagggccagg cccgccgcca
//
LOCUS       DASTFV010000116         7211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_391404, whole genome shotgun sequence.
ACCESSION   DASTFV010000116 DASTFV010000000
VERSION     DASTFV010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391404"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..751)
                     /locus_tag="VFP02_02110"
     CDS             complement(167..751)
                     /locus_tag="VFP02_02110"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HET9541846.1"
                     /translation="MDVTEAVDHAPRSRGLVLGAAAVVAVIMVALVVVLATRDPAEDR
                     VDESPLVGHVAPPLVGEVVAPEATAGTPFDLDRLRGQWVVVNFFATWCVPCVTEHPEL
                     IEFSERHAAAGDASVASVVFDADEDVDDIVDFFADNGGDWPVVDGEDVILDWAVAAVP
                     ESFLVSPDGIVAAKITGGVTADGLDDLIAEVQQA"
     gene            complement(751..2733)
                     /locus_tag="VFP02_02115"
     CDS             complement(751..2733)
                     /locus_tag="VFP02_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HET9541847.1"
                     /translation="MNGALGTAGVLLGLLGALGGAGTVLVGLQRGRPNLLRSAPAYGL
                     LVFAGAFLAMAAMERALITRDFTVLFVAENGSSRTPAVFNVATLWSALEGSIILWALV
                     LGGYLVAVLRKFRDRLDDPLVGWALVTMFVTAAFFFALMVGPADPFKSFNPPPGFDGP
                     GPNPLLQNHILMAFHPPMLYLGYVGFTVPFAFAIGALATGRVGEGWLVETRRWTLFAW
                     GFLTVGIVLGAWWSYEVLGWGGYWAWDPVENASFLPWLTGTAYLHSVMVQERRGMLRV
                     WNLSLLCATFALTILGTFLTRSGVLDSVHAFTESGIGPAFLGFFAVIVGTSVLLIGWR
                     GDRLRAPGRIDSPLSREGAFFGNNLLFAAFAFVVLLGTIFPLVVEAVNGDRISVGVPY
                     FDRMAMPIGLGLLALMAVAPVLPWRKASAELLRHRLEWPAWIGTLTVVVAVLLGARGL
                     APLLAFGLGGFAAGSAGRQLVLATRRHGWKGLVGRANGGMVVHIGVVIIAVAFAASQS
                     YGHVGEFRLGEGDSAEVGGHTVTYLGLETIERSAKTETRAQVQIDDDGVFTPSLSEFA
                     FGGQAIGTPSVRVGLTGDVYLALQALPEDGSDEILLRVVVQPLILWLWVGGTVMAVGT
                     ILAGWPGNRRRPTDPTSAGLAEEVTGPAPDPTPVTA"
     gene            complement(2780..3289)
                     /locus_tag="VFP02_02120"
     CDS             complement(2780..3289)
                     /locus_tag="VFP02_02120"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HET9541848.1"
                     /translation="MDVSHDEGESADAGDAPPLDLTPRTTPSRRRRGLAASLVLVAVV
                     AALALIAVKALGDASLFFLNADEAVEQREDLGTDRFRLQGTVVDDSVEETDDGVAFQV
                     AFNGATVDVVHRGDPPELFQPGIPVVLEGHWDGEVYASDRILVKHSSEYEAENEDRLR
                     EADEGQSSP"
     gene            complement(3280..3414)
                     /locus_tag="VFP02_02125"
     CDS             complement(3280..3414)
                     /locus_tag="VFP02_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541849.1"
                     /translation="MINQGYVVAGYVFTFLGLGAYAAWILVRGRRLSAKVPEERRRWM
                     "
     gene            complement(3411..4157)
                     /gene="ccsA"
                     /locus_tag="VFP02_02130"
     CDS             complement(3411..4157)
                     /gene="ccsA"
                     /locus_tag="VFP02_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET9541850.1"
                     /translation="MNQTTTGGSPAHTGSRGSRIVGLLAAGGAVALLVFALVLSPADR
                     IQGEAVRLMYLHVPAAICMYLCVGLLGLGSAMWLVKKTPGWDVLAAAAAEVGLVFTAL
                     TLLTGMLWGRPTWGVYWVWDARLTTTAMLAVLLAGYLALRRVPAAAESRSRRAAVAGL
                     LVVIDLPLIHYSVTWWRTLHQGETIATTDVKIDGLMLFSLVLGMAVGVLVTVWLLIHR
                     FRVAWLEEEVEQGGLDLALAERRAEAEVGA"
     gene            complement(4154..4822)
                     /locus_tag="VFP02_02135"
     CDS             complement(4154..4822)
                     /locus_tag="VFP02_02135"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HET9541851.1"
                     /translation="MLADARLIAGKDLRIERRARVATNQVAPFALLVLVLFAFALDPD
                     RGILDRATAGLYWVAVLLSTVLAVQRAYALESTDGLPDALRLSGLDPAAVFLGKTAAL
                     ALQLLVLEALLLAGVALLYTTELAGPALLAATCLAATLGLASAGSLYGVLAAGLRVRE
                     TLLPLLLLPVVAPVLIGATRAFEAALAGTPGEGWPWCGLLAIFAAIYVAFGMLAFGPL
                     MEEL"
     gene            complement(4812..5489)
                     /gene="ccmA"
                     /locus_tag="VFP02_02140"
     CDS             complement(4812..5489)
                     /gene="ccmA"
                     /locus_tag="VFP02_02140"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HET9541852.1"
                     /translation="MAPVVHLQGAVSLLGRFPALAGVDLDVEAGEVVLLRGPNGAGKT
                     TLLRVCAGLLPVTSGTAEVLGVDLGQDRRAVRHRVGFVGHGGGLYDDLSVAENVAFWG
                     RAAGATDADVAGALTRLGLDGRLRDLAVARLSAGQRRRTSIAAMVARRPELWLLDEPH
                     ASLDAEGRDLIDTLIRDSVAAGATVLMASHEDDRAAAVATRVVEVRGGTTVAPVDGAV
                     PEAPRAG"
     gene            5668..6555
                     /locus_tag="VFP02_02145"
     CDS             5668..6555
                     /locus_tag="VFP02_02145"
                     /inference="COORDINATES: protein motif:HMM:NF033747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HET9541853.1"
                     /translation="MKLARVLGAVGRVLITAGTLILLFVAYELWGTGIHTAQAQDDAR
                     DEIEQQTEAYSAEHPDPRPPRPGQAQPVPDAPPRGEPLGIIQIPAIGVSKVFFEGVEL
                     DVLSSGPGHYEGTPLPGQEGNAGIAGHRTTYGAPFNRIDELGEGDEIIISYANGSQFT
                     YVYNSTVIVAPERTDVLEYKFDNRLTLTACHPKYSAAERIVVSAVLEEPAAPFTPLPD
                     AGEAVVPLDASTSIDGQTHPKTPAVVWGIAAAMVWLTAWVVGRAWRKWPMYAIGLPIF
                     LLVLYGFFENFSYALPSSY"
     gene            6555..7112
                     /locus_tag="VFP02_02150"
     CDS             6555..7112
                     /locus_tag="VFP02_02150"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HET9541854.1"
                     /translation="MRTLRLAAVLLTAGLALSACADDDPPEWLVERATPTTTTPVPAT
                     TAVPSTTVPHPGRELAVIDLEPGVCIEDAGAFTGTEVNEITQTQAIACRLPHDAEVYL
                     RQDLPAGPDDPFPGVGALRSQAQELCRDGFEAFVGVRWTRSELEIAALWPSPPSWAEG
                     DRLVVCAVFRLDGQPLTGSALGSRT"
BASE COUNT         1157 a         2648 c         2563 g          843 t
ORIGIN      
        1 gcttcggagt cggcgaccga ctggctgcga cacgtcgggc acttgtaccc ctcggccacg
       61 tcgaacaccc gctcctcggt ggtgcggggc gtgtcgtcgg gctggagccc gaccaacagg
      121 gcgcccagga gcaccaggcc catcaccagc cacgagaacc ggcgcatcag gcctgctgga
      181 cctcggcgat gaggtcgtcg aggccatcag ctgtcacccc gccggtgatc ttggcggcca
      241 cgatcccgtc gggcgacacc aggaacgact cgggcaccgc ggcgacggcc cagtcgagga
      301 tcacgtcctc gccgtcgacc accggccagt cgccgccgtt gtcggcgaag aagtcgacga
      361 tgtcgtcgac gtcctcatcg gcatcgaaga ccacgctcgc caccgacgcg tcacccgcgg
      421 ccgcgtggcg ctcactgaac tcgatgagct ccgggtgctc cgtgacgcag ggcacgcacc
      481 aggtggcgaa gaagttgacc accacccact ggccgcggag acggtcgagg tcgaagggcg
      541 tgcccgccgt ggcctcgggg gcgacgacct cgcccaccaa cggcggggcg acatggccga
      601 ccagcgggct ctcgtcgacc cgatcctccg ccggatcgcg agtggccagg accacgacga
      661 gggcgaccat gatcacggcc acgaccgccg ccgcgccgag gaccagcccc cggctgcggg
      721 gcgcgtgatc gaccgcttcg gtgacgtcca tcaggcggtg accggcgtcg ggtcaggggc
      781 agggccggtg acctcctcgg ccagcccggc cgaggtcggg tcggtgggac gccgccggtt
      841 cccgggccac ccggcgagga tcgtgcccac ggccataacc gtcccgccga cccagagcca
      901 caggatcaac ggctggacca cgacccgcag gaggatctcg tcgctgccgt cctcgggcag
      961 ggcctgcagg gccaggtaga cgtcgccggt gagcccgacc cgtaccgacg gcgtgccgat
     1021 ggcctgcccg ccgaaggcga actccgagag gctgggggtg aacacgccgt cgtcatcgat
     1081 ctggacctgc gcccgggtct cggtcttggc cgaccgctcg atcgtctcca gcccgagata
     1141 ggtcaccgtg tggcccccga cctcggccga gtccccctcg cccaaccgga actcgccgac
     1201 gtggccgtag ctctgggagg cggcgaaggc cacggcgatg atgaccacgc cgatgtgcac
     1261 gaccatgccg ccgttggccc ggcccaccag gcccttccac ccgtggcggc gggtggccaa
     1321 caccagttgg cggccggccg acccggcggc gaacccaccg aggccgaagg cgagcagcgg
     1381 ggccaggccc cgggccccca ggaggaccgc gacgaccacc gtcaacgtcc cgatccaggc
     1441 cggccactcc agccggtggc gcagcagctc ggcgctggcc ttgcgccagg gcaacaccgg
     1501 ggccacggcc atgagggcca ggagaccgag cccgatgggc atcgccatgc ggtcgaagta
     1561 gggcaccccg accgagatcc ggtcgccgtt caccgcctcg accaccagag ggaagatcgt
     1621 gcccagcagg acgacgaagg cgaaggcggc gaacaggagg ttgttgccga agaaggcccc
     1681 ctcccgggag aggggcgaat cgatgcgccc gggcgcccgg agccggtcgc cccgccagcc
     1741 gatgagcagg acgctggtcc cgacgatcac ggcgaagaag ccgaggaagg cgggcccgat
     1801 gcccgattcg gtgaacgcgt ggaccgagtc gaggaccccg gagcgggtga ggaaggtgcc
     1861 gaggatggtc agggcgaagg tggcgcagag cagcgacagg ttccagaccc gcagcatccc
     1921 ccggcgctcc tggaccatca ccgagtgcag gtaggccgtc ccggtcagcc agggcaggaa
     1981 ggaggcgttc tcgaccgggt cccacgccca gtagccgccc caccccagca cctcgtacga
     2041 ccaccaggcc ccgagcacga tgccgacggt gaggaagccc caagcgaaca acgtccagcg
     2101 ccgggtctcg accagccagc cctcgcccac ccgtccggtg gccagcgccc cgatggcgaa
     2161 ggcgaagggc acggtgaagc cgacgtagcc caggtagagc atcggcgggt ggaacgccat
     2221 gaggatgtgg ttctggagga gcgggttggg ccccgggccg tcgaagcccg gcggcgggtt
     2281 gaacgacttg aaggggtcgg ccgggccgac catcagggcg aagaagaacg ccgcggtcac
     2341 gaacatggtg accagggccc agcccaccag cgggtcgtcg aggcgatccc ggaacttgcg
     2401 gagcacggcg acgaggtacc cgccgaggac cagcgcccac aggatgatcg agccctcgag
     2461 ggccgaccag agggtggcca cgttgaacac ggccggggtg cggctgctgc cgttctcggc
     2521 cacgaagaga acggtgaagt cccgggtgat gagggcccgc tccatggccg ccatggccag
     2581 gaacgctccg gcgaagacga ggagcccgta ggccggcgcc gaccgaagca ggttcggccg
     2641 gccccgctga agccccacca ggacggtgcc cgcgccgccc agggcgccga ggagcccgag
     2701 cagcacgccg gccgtcccga gcgcgccgtt cacgacgatc gcctggtcac gtcggcgacg
     2761 cgaaggggaa acgagccgtt cacgggctcg actggccctc gtcggcctcc cgcaggcgat
     2821 cttcgttctc ggcctcgtac tccgaggagt gcttcaccag gatccggtcg gaggcgtaga
     2881 cctcaccgtc ccaatggccc tccaggacca cggggatgcc gggctggaag agctccggcg
     2941 ggtcgccccg gtggaccacg tccacggtcg ccccgttgaa ggcgacctgg aaggcgacgc
     3001 cgtcgtcggt ctcctcgacc gagtcgtcca cgaccgtccc ctggaggcgg aagcgatcgg
     3061 tacccaggtc ctcgcgctgc tcgaccgcct cgtcggcgtt gaggaagaag agcgaggcgt
     3121 cgcccagggc cttcaccgcg atgagggcca gggcggcgac gaccgcgacc aggacgagcg
     3181 aggcggcgag gccgcggcgg cgccgggacg gggtggtcct cggtgtgagg tcgaggggcg
     3241 gcgcgtcgcc ggcgtcggcg gattcgccct cgtcgtggct cacatccacc gacgacgctc
     3301 ctcggggacc ttggcggaga gacgacgacc ccgtaccagg atccacgccg cgtaggcgcc
     3361 gaggccgagg aaggtgaaca cgtagccggc gacgacgtag ccctggttga tcacgccccc
     3421 acctccgctt cggcccgccg ctcggcgagg gcgaggtcga ggccgccctg ctcgacctcc
     3481 tcctccagcc aggccactcg gaaccggtgg atgagcagcc acaccgtcac caggacgccg
     3541 accgccatgc ccaggacgag gctgaacagc atcagcccgt cgatcttcac gtcggtggtg
     3601 gcgatggtct cgccctggtg cagggtccgc caccaggtga cggagtagtg gatgaggggg
     3661 aggtcgatca cgaccaggag cccggccacg gccgcccgtc gggagcggga ttcggcggcg
     3721 gccgggaccc ggcgcagagc caggtatccg gccaggagca cggccagcat ggccgtggtg
     3781 gtcaggcggg cgtcccacac ccagtagacg ccccaggtcg ggcgacccca cagcatcccg
     3841 gtgagcaggg tcaacgcggt gaagaccagc ccgacctcgg cggccgccgc ggccaggacg
     3901 tcccagcccg gcgtcttctt gaccagccac atggcgctcc ccagcccgag caggccgacg
     3961 cagaggtaca tgcagatggc cgccggcacg tgcaggtaca tgaggcggac cgcctccccc
     4021 tggatgcggt cagccgggct gagcacgagg gcgaagacca ggagggccac cgcaccgccg
     4081 gccgccagga ggcccacgat ccggctgccc cgcgacccgg tgtgggccgg cgaccccccg
     4141 gtcgtggtct ggttcacagt tcctccatga ggggcccgaa ggcgagcatc ccgaacgcta
     4201 cgtagatcgc cgcgaatatg gccagcaggc cacaccacgg ccaaccttcg ccgggcgtcc
     4261 ccgccagcgc cgcctcgaag gcccgggtgg ccccgatgag cacgggggcc acgaccggga
     4321 gcaacagcag cggcagcagg gtctcccgga cccggaggcc ggcggcgaga acgccgtaga
     4381 ggctaccggc ggaggccagc cccagggtcg cggccaggca ggtagccgcg agcagggccg
     4441 gaccggccag ctcggtcgtg tagagcagcg ccaccccggc cagcaacagg gcctcgagga
     4501 cgagcaactg gagcgccagg gccgccgtct tgcccaggaa gaccgcagcc ggatcgaggc
     4561 cggagaggcg aagggcgtcc ggcaggccgt cggtcgactc cagggcgtag gcccgctgga
     4621 cggccagcac cgtcgacagc aggacggcca cccagtagag gccggcggtg gcccggtcga
     4681 ggattccccg gtccgggtcg agggcgaagg cgaagagcac caggaccaga agcgcgaacg
     4741 gggccacctg gttcgtcgcc acccgagccc gccgttcgat gcgcaggtcc ttaccggcga
     4801 tcaggcgcgc gtcagccagc acggggagcc tccgggacgg cgccgtcgac cggggccacg
     4861 gtggtgcccc cgcggacctc gacgacgcgg gtggccacgg ccgccgcccg gtcgtcctcg
     4921 tggctggcca tcagcaccgt ggcccccgcc gccacggagt cccggatgag ggtgtcgatc
     4981 aggtcccggc cctcggcgtc gaggctggcg tggggctcgt ccagcaacca cagctcggga
     5041 cggcgggcga ccatggccgc gatggaggtc cgccgccgct ggccggcgga gagtcgggcc
     5101 acggccaggt cccggaggcg gccgtcgagg ccgagccggg tcaatgcccc cgccacgtcg
     5161 gcgtcggtgg cgcccgccgc ccgaccccag aaggccacgt tctcggccac gctgaggtcg
     5221 tcgtagaggc cgccgccgtg gcccacgaaa ccgacccggt ggcggacggc gcgccggtcc
     5281 tggccgaggt ccacaccgag gacttcggcc gtgccgctgg tcacgggcag gaggccggca
     5341 cacacccgca ggagggtggt cttgccggcg ccgttcgggc cgcggagcag gaccacctca
     5401 ccagcctcga cgtcgaggtc gacaccggcg agtgcgggga accgcccgag caacgagaca
     5461 gcaccctgga ggtggacgac aggggccatg cgcgggccaa ggctactgat gcgcaacgcc
     5521 gaagcccggt cccggcgctg cgtagtcgga gcggagcagc tcaccagatc cgcggcatcg
     5581 gcgacgccgg acgtcgtgaa cgaagtgaac gatgtcgagg gtgacggtgc ccgagtgggg
     5641 tcccaaggca ccggtaccct cccggccgtg aagttggcgc gcgtcctcgg tgcagtgggc
     5701 cgcgtcctga tcaccgcggg caccctgatc ctgctcttcg tcgcctacga gctctggggc
     5761 acgggcatcc acaccgccca ggcccaggat gacgcccggg acgagatcga gcagcagacc
     5821 gaggcctaca gcgccgagca cccggatccg cgtccgccgc gacccggcca ggcccagccg
     5881 gtgcccgacg caccgccccg gggcgagccc ctcgggatca tccagatccc ggccatcggc
     5941 gtgagcaagg tcttcttcga gggggtcgag ttggacgtgc tgtccagcgg ccccggccac
     6001 tacgagggca ctcccctgcc gggccaggag ggcaacgccg ggatcgccgg gcaccgcacg
     6061 acctacggcg ccccgttcaa ccgcatcgac gagttgggcg agggcgacga gatcatcatc
     6121 agctacgcca acggctcgca gttcacctat gtgtacaact cgacggtcat cgtggccccg
     6181 gagcgcaccg acgtgctcga gtacaagttc gacaaccgcc tcaccctgac ggcctgtcac
     6241 ccgaagtaca gcgccgcgga gcgcatcgtg gtgtccgccg tcctcgagga gcccgcggcc
     6301 cccttcaccc cgctccccga tgcgggagaa gcggtcgtcc cgctcgacgc cagcaccagc
     6361 atcgacggcc agacccaccc caagaccccg gccgtcgtgt gggggatcgc cgccgccatg
     6421 gtctggctca ccgcctgggt cgtgggccgg gcctggcgga agtggccgat gtatgccatc
     6481 gggctgccca tcttcctcct cgtgctctac ggcttcttcg agaacttctc ctacgccctg
     6541 ccctccagct actgatgcgc accctccgcc tcgccgccgt cctcctcacc gctggtctcg
     6601 ccctgagcgc gtgcgccgac gacgaccccc cggagtggct ggtcgagcgg gccacgccca
     6661 cgaccacgac cccggtcccc gcgacgaccg ccgtgccgtc gaccacggtg ccccacccgg
     6721 gccgcgagct ggcggtcatc gacctcgagc ccggcgtctg catcgaggac gccggtgcct
     6781 tcaccgggac cgaggtcaac gagatcaccc agacccaggc catcgcctgt cgcctcccgc
     6841 acgatgccga ggtgtacctc cgccaggacc tgcccgccgg tccggacgat cccttccccg
     6901 gtgtgggcgc gctgcggagc caggcccagg agctctgccg agacggcttc gaggccttcg
     6961 tcggggtgcg ttggacccgg tccgaactgg agatcgccgc gctgtggccg tcgccgccgt
     7021 cgtgggcgga gggcgaccgg ctcgtggtgt gcgccgtgtt ccgcctcgac ggccagccgc
     7081 tgacgggctc ggccctgggg tcccgcacct aaccgagccg ccccgtcgct tacgctcccg
     7141 cgccagtgcc gatccgccgt gtcctcatcg ccgcgttggc gtgcctgctg ccgctcgccg
     7201 cctgctccgc c
//
LOCUS       DASTFV010000117         2746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_391998, whole genome shotgun sequence.
ACCESSION   DASTFV010000117 DASTFV010000000
VERSION     DASTFV010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391998"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VFP02_02155"
     CDS             <1..949
                     /locus_tag="VFP02_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET9541855.1"
                     /translation="SALAAPLADYSVMTERAALFTAGPLLVKAATGEDVTPEALGGPQ
                     VAVASGVVQDVVPDDDAAIDRARAWIGYFDGQVGEGDDVGPRPLGDLLEVIPPDPRRP
                     YAMVEVVDRLADGGSVLVAGAGFGGGMLTALIRLGGRTVAVVANDPSVRAGAIGRDEA
                     DKAARFLETVAPFGLPVVFLADNPGVMAGTQAERDGVLRAAAGLFAAQRRYPGSKLHV
                     TLRKAFGFGSSAMAMNPFDHQALTLAFPGVLLGAMPAESGGRAAGLTADEQAAAEILQ
                     AGGPYRSAHTMGYDDVVDPRDLRDRLLAGLVMAGGRGAR"
     gene            complement(921..1679)
                     /locus_tag="VFP02_02160"
     CDS             complement(921..1679)
                     /locus_tag="VFP02_02160"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET9541856.1"
                     /translation="MTTTTVITAVAPPPFGQDPDPVVIGHRGAAGHHPDNSLEGFAAA
                     RDLGATWVELDVRLSADGVVVLSHDPATAAGSLVTETPSAQLATEGLTTLAEALPVIE
                     EHALGVDVEIKNLPWEDDYDPRLRLVDATLEVLDEASPLGPVVLSSFNQGAIDRVRAQ
                     AGDAYATALITPGAGDARALRARVLAAGHDGVVIDHEAVDDEILHTLSTGGLTLWAYT
                     VDDPMTAAVLVADGVSGIVTDVPDEISERLDLLP"
     gene            1986..>2746
                     /locus_tag="VFP02_02165"
     CDS             1986..>2746
                     /locus_tag="VFP02_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HET9541857.1"
                     /translation="MRSEAVAIGDGIYNWDGLGQTRVALIGREGRARFVVRAENDGNG
                     IDDLVVQGTRNTTNFGIRYFVGDQDVSARVRNGIFRFADVGPGQHRALNVEVRANPSA
                     PLRSKVIVRVALRSGTDGTLRDRVKAVVYRSQGVETLIEGRPFTGQATAERWADDQEA
                     SARFVLNAALYWELAPARGIRPEVAYAQSAKETAYGHFGGVIDASFHNPCGLKTTTGG
                     DNDDPNAHMRFVNWRQGITACIDHLGLYAGAPGYP"
BASE COUNT          362 a          974 c         1030 g          380 t
ORIGIN      
        1 cagcgccctg gccgccccgc tggcggacta ctcggtgatg accgagcggg ccgccctgtt
       61 caccgccggt cccctgctgg tcaaggcggc caccggtgag gacgtgaccc ccgaagccct
      121 cggggggccg caggtggcgg tggcgtcggg cgtggtccag gacgtggtgc ccgatgacga
      181 cgcggccatc gaccgggccc gcgcctggat cggctacttc gacggccagg tgggggaggg
      241 ggacgacgtc ggtccccgtc cgctgggcga cctgctcgag gtgatcccgc ccgatccccg
      301 gcgcccttac gcgatggtcg aggtcgtgga tcgcctggcc gacggcggat ccgtcctggt
      361 ggccggggcg ggcttcggcg gcgggatgct gaccgccctg atccgcctcg ggggccggac
      421 cgtggccgtg gtggccaacg acccgtccgt ccgggccggg gcgatcggcc gggacgaggc
      481 ggacaaggcc gctcgcttcc tcgaaaccgt ggcgccgttc gggctgccgg tggtcttcct
      541 ggccgacaac cccggcgtga tggcagggac gcaggccgag cgggacgggg tgctcagggc
      601 cgccgccggg ctcttcgccg cccagcgccg atacccgggc tcgaagctgc acgtgaccct
      661 gcgcaaggcc ttcggcttcg gctcgtcggc catggccatg aaccccttcg accaccaggc
      721 cctgaccctg gccttccccg gggtcctgct cggggccatg cccgccgaga gcgggggccg
      781 ggcggcgggc ctcaccgccg acgagcaggc cgccgcggag atcctccagg ccggcggtcc
      841 ctaccgttcg gcccacacca tgggctacga cgacgtcgtc gacccgagag acctccgtga
      901 ccgtctgctc gccggcctgg tcatggcagg aggtcgaggc gctcgctgat ctcgtcgggc
      961 acatcggtca cgatcccgct cacgccgtcg gcgacgagga cggccgccgt catggggtcg
     1021 tcgacggtgt acgcccagag ggtcaggccg cccgtcgaca gggtgtgcag gatctcgtcg
     1081 tcgaccgcct cgtggtcgat gacgacgccg tcgtggccgg cggcgagaac ccgggcccgg
     1141 agcgcacgcg cgtctccggc gcccggggtg atgagggccg tggcgtaggc gtcgcccgcc
     1201 tgcgcccgga cgcggtcgat ggccccctgg ttgaacgacg acagcaccac cggccccagc
     1261 ggcgaggcct cgtccaggac ctcgagcgtc gcgtcgacga ggcgcagtcg ggggtcgtag
     1321 tcgtcctccc agggcaggtt cttgatctcg acgtcgaccc cgagggcgtg ttcctcgatc
     1381 accgggagag cctcggcgag ggtggtcagc ccctcggtgg cgagctgggc ggagggcgtc
     1441 tcggtcacca acgagccggc ggccgtggcc ggatcgtgac tcagcacgac gacgccgtcg
     1501 gcggacaggc gcacgtcgag ctcgacccag gtggcgccga ggtcccgcgc cgcggcgaag
     1561 ccttcaagcg agttgtcggg gtgatggccg gcggcgccgc ggtggccgat cacgacgggg
     1621 tcggggtcct ggccgaacgg gggtggggcg acagcggtga tgacggtggt cgtggtcacc
     1681 gccgcgctgg tgggctctgc cgtctggtcg cgcccaccgt cgtcggagca gccgctgacc
     1741 agcagtacgg cggcgaagct gacggcgaac gggtgcgtcg acgagcgggc gatcgccgga
     1801 gcgtaggcgc cggttcccca cgaggtcgcg ggggtaacct cggggtcatg ttcggcggag
     1861 cccggccctt ggcggccgtc tttctcatga tggcgctagg cctccagatc ggggtggcgg
     1921 aggcgcagac aacggatccg ggtccttgtg cccctccgtg ccagcagccg gacggtcgca
     1981 tccggttgcg gagcgaggcc gtcgccatcg gggacgggat ctacaactgg gacggcctgg
     2041 gccagacccg ggtggcgctc atcggccgcg aggggcgggc tcgcttcgtc gtgcgggccg
     2101 agaacgacgg caacggcatc gacgacctgg tggtgcaggg gacccgcaac accaccaact
     2161 tcggcatccg gtacttcgtc ggcgaccagg acgtctcggc ccgggtccgc aacggcatct
     2221 tccgattcgc tgacgtcggc cccggccagc accgggcgtt gaacgtcgag gtccgggcca
     2281 acccttcggc gccgctgcgc tcgaaggtca tcgtccgcgt ggcgctgcgg tccgggaccg
     2341 acgggaccct acgggatcgg gtcaaggctg tcgtctaccg cagccagggc gtcgagaccc
     2401 tgatcgaggg ccggcccttc accggccagg ccaccgccga gcgctgggcc gatgatcagg
     2461 aagccagcgc ccgcttcgtc ctgaacgcgg cgctctactg ggagctggcc ccggcccggg
     2521 gcatccggcc cgaggtggcc tacgcccagt cggccaagga gacggcctac ggccacttcg
     2581 gtggggtcat cgacgccagc ttccacaacc cctgtggcct gaagaccacc accggcggcg
     2641 acaacgacga tcccaacgcc cacatgcgct tcgtgaactg gcgccagggg atcacggcct
     2701 gcatcgacca cctcggcctc tacgccggcg cccccggcta cccgag
//
LOCUS       DASTFV010000118         3016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_399618, whole genome shotgun sequence.
ACCESSION   DASTFV010000118 DASTFV010000000
VERSION     DASTFV010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399618"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1992)
                     /gene="ligD"
                     /locus_tag="VFP02_02170"
     CDS             complement(<1..1992)
                     /gene="ligD"
                     /locus_tag="VFP02_02170"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET9541858.1"
                     /translation="MATARRRRLPFPSALPAHRVGDAWRLEADGRELRLSNLDKVYWP
                     DDGITKGDLLAYYWNVAELLLPHLHDRPLTMYRMPNGLAGEPFYEKQVPAHAPDWLPT
                     AAVATEEDSRVVEFVLAQDLASLLYVVNLGCIEMHPLHARAGSLDRPDYAFFDLDPFE
                     PYTYEDVRTVAKLVKVVLDGLGLRGYAKTSGATGMQVFVPLDGTHTHSDAREFVDRVG
                     RLVVRAYPEKATMAWPVAERAGKVFIDHQMNRAGANIASVYSLRPLPGAPVSTPLDWD
                     ELDDDLEPGDFRIDNVWERFAAGDRFRPVLDDKQSLSEAMEALGLTPGATDPPRERSR
                     PRFPPKRSARPTGRASLDEYAAKRDFQRTPEPPSPGSTPAPPATAGTPQPHPPVDGAP
                     TGRVADGGNGGDRLSTAPAEGPHQAEADPAEGPHQAEADPGPYAAEADPNGLPSEAHP
                     AGLTEVAALEPGRRFTIQQHHARRLHHDVRFEQDGVLVSFAVPKRLPEEPGVRHLAVN
                     TEDHPLDYLTFSGSIPAGEYGGGEVRLFDLGSYEPLEVADGKLTVRLNGTRYRGAEYH
                     LFRTRDRDWLVILKGKVSLPQPAPPPTYEPMMAVLTAEPFDDDEWLFEVKWDGHRCLA
                     NLGQATRLTSRTRRDMTAQFPELIDMHRQLAARNAVVDGE"
     gene            2115..2945
                     /locus_tag="VFP02_02175"
     CDS             2115..2945
                     /locus_tag="VFP02_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET9541859.1"
                     /translation="MKTIWKGAISFGLVTIPVRVYGATEAKSLRFNQLHEPDGGRIRY
                     KRVCSIDGEEVDYSEIVKGYEYEKDHYVTLTDEELDSLPVASAKAIEIERFVEADEID
                     PIYFQKAYYLVPEGTGVKAYHLLREAMADDGKVALAKVAFRDKEHLATLRLRDNVFVL
                     ETMYWPDEIRVPKFDVLDESVELRPQEIRMAKSLIDSLTDVFQPEEFRDEYRAALEEL
                     VQKKVQGEEITYTEEAEPSKVVDLMEALKASVEAAKAGKPAPERKTSSSRRRKKAAGE
                     "
BASE COUNT          429 a         1007 c         1130 g          450 t
ORIGIN      
        1 ctcgccgtcg acgaccgcgt tgcgggccgc cagctggcgg tgcatgtcga tcagctcggg
       61 gaactgggcg gtcatgtcgc gcctggtccg cgacgtcagc cgggtcgcct ggcccaggtt
      121 ggccaggcag cggtggccgt cccacttgac ctcgaacagc cactcgtcgt cgtcgaaggg
      181 ctcggcggtg agcacggcca tcatcggctc gtaggtcggg ggcggcgccg gctgggggag
      241 cgacaccttg cccttgagga tgaccagcca gtcgcggtcc cgggtccgga acaggtggta
      301 ctcggccccc cggtaccggg tcccgttgag gcggacggtg agcttgccgt cggccacctc
      361 cagcggctcg tagctgccga ggtcgaacag ccggacctcg cccccgccgt actcgccggc
      421 cgggatcgac ccgctgaagg tcaggtagtc gagcgggtgg tcctcggtgt tgacggcgag
      481 gtggcgcacc cccggctcct cgggcagccg cttgggcacg gcaaaggaga ccagcacccc
      541 gtcctgctcg aagcgcacgt cgtgatggag ccgccgggca tggtgctgct ggatggtgaa
      601 ccgccgcccc ggttcgaggg ccgccacctc ggtcaacccc gccggatgcg cctcgctcgg
      661 cagcccgttc gggtccgctt cggccgcata gggtcccggg tccgcctcgg cctggtgcgg
      721 tccctcggcc gggtccgcct cggcctggtg cggtccctcg gcgggggctg tggacagccg
      781 gtcgcccccg ttcccgccgt cggctaccct cccggtaggg gccccatcca ccggtgggtg
      841 gggttggggg gtgcccgccg ttgcgggggg tgccggggtg ctccccggcg aggggggttc
      901 cggggtgcgc tggaagtccc gcttggcggc gtactcgtcc aggctggccc ggcctgtggg
      961 gcgggccgac cgctttggcg ggaaccgagg gcggctccgt tcgcggggcg ggtcggtggc
     1021 gccgggggtg agccccaacg cctccatggc ctcggacagc gactgcttgt cgtccaggac
     1081 cgggcggaac cggtcgccgg cggcgaaccg ctcccagacg ttgtcgatcc ggaagtcgcc
     1141 cggctccagg tcgtcgtcca gctcgtccca gtccagcggg gtcgacaccg gcgccccggg
     1201 cagcggccgc agcgagtaga cgctggcgat gttggccccg gcccggttca tctggtggtc
     1261 gatgaacacc ttgcccgccc gttccgcgac cggccaggcc atggtcgcct tctcggggta
     1321 ggcgcggacg accagccggc ccacacgatc cacgaactcg cgggcgtcgc tgtgggtgtg
     1381 ggtgccgtcg aggggcacga acacctgcat gccggtcgcc cccgaggtct tggcgtagcc
     1441 ccgcaggccg agcccgtcca gcaccacctt gaccagcttg gccacggtgc ggacgtcctc
     1501 gtaggtgtac ggctcgaagg ggtcgaggtc gaagaaggcg tagtcgggcc ggtccaggga
     1561 gccggcccgc gcgtgcaggg ggtgcatctc gatgcagccg aggttgacca cgtacagcag
     1621 gctggccagg tcctgggcga gcacgaactc gacgacccgg gagtcctcct cggtggccac
     1681 ggccgcggtg ggcagccagt cgggggcgtg ggccggcacc tgcttctcgt agaacggctc
     1741 gccggccagg ccgttcggca tccggtacat ggtcagcggc cggtcgtgga ggtgggggag
     1801 gagcagctcg gccacgttcc agtagtaggc gagcaggtcg cccttggtga tgccgtcgtc
     1861 cggccagtag accttgtcca ggttggacag gcgcagctca cggccgtcgg cctccagccg
     1921 ccaggcgtcg ccgacccggt gggcgggcag cgccgacgga aacggcaggc ggcggcgcct
     1981 ggcggtagcc aacgtgcccg ggcctccttc agttgcgttg ctcgccagcg tacagttgtc
     2041 cctggtccgt gaccgtcgcc ttccgacgtg cccgccgctc gcggagtgtc cgacgtcaaa
     2101 ggaggcctgg cttcgtgaag acgatctgga agggggcgat ctccttcggg ttggtcacca
     2161 tccccgtccg ggtgtatggg gccaccgaag cgaagagcct ccggttcaac cagctgcacg
     2221 agcctgacgg cggccggatc cgctacaagc gggtgtgctc gatcgacggg gaagaggtcg
     2281 actactcgga gatcgtcaag gggtacgagt acgagaagga ccactacgtg accctgaccg
     2341 acgaggagct cgactcgctg ccggtggcct cggccaaggc gatcgagatc gagcgcttcg
     2401 tcgaagccga cgagatcgac cccatctact tccagaaggc ctactacctg gtgcccgagg
     2461 gcaccggggt caaggcctac cacctgctgc gcgaggccat ggccgacgac ggcaaggtgg
     2521 ccctggccaa ggtcgccttc cgcgacaagg agcacctggc caccctgcgg ctccgcgaca
     2581 acgtgttcgt gctggagacg atgtactggc cggacgagat ccgcgtgccc aagttcgacg
     2641 tcctggacga gtcggtcgag ttgcggcccc aggagatccg catggccaag agcctgatcg
     2701 acagcctcac cgacgtcttc cagcccgagg agttccgcga cgagtaccgg gcggcgctcg
     2761 aggagctggt ccagaagaag gtccagggcg aggagatcac ctataccgaa gaggccgagc
     2821 cgtctaaggt ggtcgacctg atggaggcgc tgaaggcgag cgtcgaggcg gccaaggccg
     2881 gcaagccggc cccggagcgc aagacgagca gcagccggcg gcgcaagaag gcggcaggcg
     2941 agtagggaag tagccggtag cgggcgtccc ggtccggggc gtcgtggggg gtggacgtgg
     3001 cgcttgacct ctgccc
//
LOCUS       DASTFV010000119         2790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_406442, whole genome shotgun sequence.
ACCESSION   DASTFV010000119 DASTFV010000000
VERSION     DASTFV010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406442"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VFP02_02180"
     CDS             <1..763
                     /locus_tag="VFP02_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9541860.1"
                     /translation="LIRWEDPERGIVSPGEFIPALENTGLIVPVGTWVLEQACRQAHE
                     WARTYSDQVPLMVTVNVSARQLAQADFGEVLRRVLGETGVDPGTICLEITEGAVMADV
                     DAAWTMLRSAKALGVYLALDDFGTGFSSLSFLQKFRPDIVKIDKSFVDGLGQSREDTT
                     IIEHVVGMARGLGMVTVAEGVETADQVRYLHSLECNMAQGYFYSRPQPPSVIDRILGQ
                     PPNDRWRRDRVAAEAAQALPAPAVTPTMRTPAPAY"
     gene            complement(764..2197)
                     /locus_tag="VFP02_02185"
     CDS             complement(764..2197)
                     /locus_tag="VFP02_02185"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9541861.1"
                     /translation="MRFTVIGHACLRIETSGPTILVDPWLTGSCYWRSWWHFPADADP
                     TPELLAPDVVYLTHHHFDHFHYPSMRQVDKGAEVLCARFGVDVMADEVRDIGFRTVSE
                     LPHGEIRELAPGVRVASYQYGFDDTAFVVADGDTVLADLNDCKIRGRALRQIAEDFGP
                     VTFLLKTHSWAQSYPHLYEAEDAEDLTLLKRQTYLDEFIDATRTLQPRFAVPFANMVA
                     FLHPESWAMNAEMITPDEVRAAFERADGLERTALVAMSPGDTWSDEDGFDVSGTRWWD
                     DRLPRMTQMAAAIAPTLAATAAEEAQRSLAFTDFEHHLSAFLQAVPRPALRMLLKRPI
                     VFEVPGDEQPYWVVDGRRRRIWRQATPPADRANLIRVAPAVLADAIDKRILHFVHGSM
                     RIRVALRPGGVNEDLAFWGLLMIWEIGYLPVRNLASRRFLDAVWRRRREAVGSLGMFR
                     GSGSPLKRLSEGFAVPTDDDRGATRAA"
     gene            complement(2330..>2790)
                     /locus_tag="VFP02_02190"
     CDS             complement(2330..>2790)
                     /locus_tag="VFP02_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541862.1"
                     /translation="LEWLVGPIDKVSAWTREFHAIDGIEDAATASFSFASGAVGSMVS
                     VWHDVLERPSLRHVEILAERLHVTVEGDWFGPVRWTQTGQGEQVLDGADLLAALVPAL
                     PEGGNPDGEFLAAVAEGRPASPAVGEALRAHRLVDALYRSAAADGTPVAP"
BASE COUNT          433 a          982 c         1001 g          374 t
ORIGIN      
        1 cctcatccgc tgggaggacc ccgagcgggg catcgtcagc ccgggcgagt tcatcccggc
       61 cctggagaac accggcctca tcgtccccgt cgggacctgg gtgctggagc aggcctgtcg
      121 ccaggcccac gagtgggcca ggacctacag cgaccaggtc ccgctcatgg tcacggtcaa
      181 cgtgtccgcc cgccagctgg cccaggccga cttcggcgag gtgctgcgcc gggtgctggg
      241 cgagaccggg gtcgaccccg ggaccatctg cctcgagatc accgagggcg cggtcatggc
      301 tgacgtcgac gccgcctgga cgatgctgcg ctcggccaaa gccctcggcg tctatctcgc
      361 cctcgacgac ttcgggaccg ggttctcgtc cctcagcttc ctgcagaagt tccggccgga
      421 catcgtcaag atcgacaagt ccttcgtcga cggcctcggt cagagtcgag aggacaccac
      481 gatcatcgag cacgtggtgg gcatggcccg cggcctcggc atggtgacgg tggccgaagg
      541 cgtggagacg gccgaccagg tgcgttacct gcacagcctc gagtgcaaca tggcacaggg
      601 ctacttctac agccggcccc agcccccctc ggtcatcgac cgcatcctcg gccagccccc
      661 gaacgaccgc tggcgccgcg accgggtcgc ggccgaggcg gctcaggccc tgccggcccc
      721 ggccgtgacc ccgaccatgc ggacgcccgc ccccgcctac tgatcaggcg gcccgcgtcg
      781 ccccgcggtc gtcgtcggtc ggcaccgcga agccctcgga caaccgcttg agcggtgaac
      841 ccgacccccg gaacattccc aaggagccga ccgcttcacg acgacgtcgc cagacggcgt
      901 ccaggaagcg acgagaagcc aggttccgca ccggcaggta gccgatctcc cagatcatga
      961 gcaggcccca gaaggcgagg tcctcgttca ccccgccggg acgcagggcc acgcggatgc
     1021 gcatggagcc gtggacgaag tgcaggatcc gcttgtcgat ggcgtcggcc aggaccgccg
     1081 gggcgacgcg gatcaggttg gcccggtccg ccggaggggt ggcctggcgc cagatgcggc
     1141 gacggcggcc gtcaaccacc cagtacggct gctcgtcgcc gggcacctcg aagacgatcg
     1201 ggcgcttcag cagcatccgc agcgccggtc ggggcaccgc ctggaggaac gccgagaggt
     1261 ggtgctcgaa gtcggtgaag gcgagggagc gttgggcctc ctcggccgcg gtggcggcca
     1321 gggtcggagc gatggccgcc gccatctggg tcatgcgagg caggcggtcg tcccaccagc
     1381 gggtcccgct cacgtcgaag ccgtcctcgt cggaccacgt gtcgccgggg ctcatggcca
     1441 ccagcgcggt gcgctccagg ccgtcggccc gctcgaaggc cgcccgcacc tcgtcgggcg
     1501 tgatcatctc ggcgttcatg gcccaggact cggggtggag gaaggccacc atgttggcga
     1561 agggcacagc gaagcggggc tgcagggtcc gggtggcgtc gatgaactcg tcgaggtagg
     1621 tctggcgctt caggagggtc aggtcctcgg cgtcctcggc ctcgtacagg tgggggtagg
     1681 actgggccca cgagtgggtc ttgagcagga acgtcaccgg cccgaagtcc tcggcgatct
     1741 ggcgcagggc ccggccccgg atcttgcagt cgttgaggtc ggccaggacg gtgtccccgt
     1801 cggccacgac gaacgcggtg tcgtcgaagc cgtactggta ggaggccacc cgcacgccgg
     1861 gggccagctc ccggatctcg ccgtggggca gctcgctcac cgttcggaac ccgatgtccc
     1921 tcacctcgtc ggccatcacg tccacgccga agcgggcgca gaggacctcg gctcccttgt
     1981 cgacctgacg catcgacggg tagtggaagt ggtcgaagtg gtggtgggtc aggtagacga
     2041 cgtcgggcgc gaggagctcg ggcgtcgggt cggcgtcggc cgggaagtgc caccaggacc
     2101 gccagtagca cgacccggtg agccaggggt cgaccaggat cgtggggccc gaggtctcga
     2161 tgcgcaggca ggcgtgcccg atgacggtga accgcacccc ggcaggctac ggagccgagc
     2221 cgctaggcga ggcgaccaat gagcgcagcg ctccattcgc cgagcgccgc gaggcgaacc
     2281 agccgttcac cgcgcgaacc ttgtacctgt ccgcggggtc ccagggcgct cagggagcga
     2341 ccggggtgcc gtcggcggca gccgagcggt agagggcgtc cacgaggcga tgggcccgga
     2401 gtgcctcccc caccgcaggc gacgccggtc gaccctcggc cacggcggcc aggaactcac
     2461 cgtcggggtt gcccccttcc ggcagcgccg ggacgagggc ggccaggagg tcggccccat
     2521 cgagcacctg ctcgccctgc ccggtctggg tccagcgcac cggcccgaac cagtcgccct
     2581 ccaccgtgac gtggagccgc tcagcgagga tctcgacgtg gcgcaggctg ggccgttcca
     2641 ggacgtcatg ccagaccgac accatcgagc ccaccgcacc ggaggcgaac gagaagctcg
     2701 ccgtcgccgc gtcctcgatg ccgtcgatgg cgtggaactc ccgggtccag gcactgacct
     2761 tgtcgatcgg acccaccagc cactcgagga
//
LOCUS       DASTFV010000120         4169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_412768, whole genome shotgun sequence.
ACCESSION   DASTFV010000120 DASTFV010000000
VERSION     DASTFV010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412768"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFP02_02195"
     CDS             <1..190
                     /locus_tag="VFP02_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541863.1"
                     /translation="GAMAAALADAGLEAPAEPEDAEAPAGPEAEVAEAPAEPDGAEAA
                     PEPEAEVAEAPDEDDATA"
     gene            409..1275
                     /gene="cpaB"
                     /locus_tag="VFP02_02200"
     CDS             409..1275
                     /gene="cpaB"
                     /locus_tag="VFP02_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HET9541864.1"
                     /translation="MSSRRTMILIAAIAVGCIAAFVVLKYVSGVEERANGDAERVSVL
                     VVRADIPQGVPGEQAVQEGWIVEDEIAREFLPATRITDFDQITGKVALNTLAANQVLV
                     EGMFVDPTTNSIGFGRRLEENHVAYTISVDSTSGVAGLLVPGDLVDIFATTGEGEPTD
                     AEVSSDTAEQQGIFGQNARLLYHQVRILAIGTETAPQPGEEQAAAEGEDAAAEAPQAS
                     GLLTLDLPIDAAQLIASVDSGSLRLALTGPDYQAQPIPRIDLNDLAELPGETDARTRF
                     GTLTPYGPDGPQ"
     gene            1315..2652
                     /locus_tag="VFP02_02205"
     CDS             1315..2652
                     /locus_tag="VFP02_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HET9541865.1"
                     /translation="MSNLFSDPLGSTDSFGGAAGVELAPIGVSVPTGYPGGDAFGGSN
                     GTMGLEPLSSAPDLSHLPLATVDVDEMVRTQLAMQLGTHAVPFPSIEALATKLTGTPL
                     VVVLGPSMASPDQLVVVERLTRPRPEIGAILIAEQLTTEVLQQALRAGVRDVLPLQDL
                     SQLAMTVKRVGDMLGSGSQQPAIMDPNNVHGAPEPGGDGQVITVFSTKGGAGKSVIAC
                     NVAVRLAKKSNRPVALVDADLQFGDVAVMLKLAPQHTIVDAVGSLDRGMDAAMLQSLL
                     VQHDASGLYVLPAPLEPAFADQIRGEEMVRIVEVLRSFCSHVIIDTPAYFNDVVLGLI
                     EISDDVLLVAGMDIPNIKNVKIGLQTLRLLNTPMSKLRLVLNRANSKVKLDVSEVEKT
                     LQVKADALIPSDVVVPQSVNKGIPVVLAAPKAAVTRAIEQLADTFLIAETAGR"
     gene            2681..4048
                     /locus_tag="VFP02_02210"
     CDS             2681..4048
                     /locus_tag="VFP02_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HET9541866.1"
                     /translation="MSLYKRLQDGGGAPAGQSGLRDPVLDELRHKIHHVLIDELGPIL
                     YDKRISEEDLRRRVHEQLHAALAQERAPLSAADKAQLIQDVSDDILGYGPIDRLLNDE
                     ELTEVMVNGPSSVYIERSGKLEKTDARFVDEEHLRRIIDKIVSEVGRRLDESSPMVDA
                     RLPDGSRVNAVIHPLAIGGPFLTIRKFSKDPFQIDDLIRFGTLNAASARFLQACVVGR
                     LNLIVAGGTGTGKTTTLNVLSSFIPADERIVTIEDAKELQLHQDHVLALEARPPNIEG
                     RGQVTIRDLVKNALRMRPDRIVVGECRAGEALDMLQAMNTGHDGSITTIHSNTPRDTL
                     SRIETMTLMAGFDLPVRAIREQMASALDCIVQLTRLRDGTRRITHVTEVQGMEGDVIT
                     LQDVFLFDYGAGVDERGKFRGHLKATGVRPKFTEKLQDLGIRLGPEVFQPEPSGGHQM
                     IGSAR"
BASE COUNT          685 a         1559 c         1338 g          587 t
ORIGIN      
        1 cggcgccatg gccgccgccc tggccgatgc cggcctggag gcccccgccg agcccgagga
       61 cgccgaggcc ccagccggcc ccgaggcgga ggtcgccgag gcgcccgccg aacccgacgg
      121 ggccgaggcc gcacccgagc ctgaggcgga ggtcgccgag gctcccgacg aggacgacgc
      181 caccgcctga tccggccgcc tgagggcggc accagttccg aactcgaggc gacagctcac
      241 ggattccgtg ggttgtcgcc tcgaggtcgt tttgaggtcg cgacgaggcg ccgcgccttt
      301 cgccattccc gtttggccaa tcgctaagca cccggaaggt ccggccgacc tagtgagaga
      361 cacaggccgg gcccaccggc acaggcgcaa ccagcaaaga ggtcggacgt gagctctcgc
      421 agaaccatga tcctcatcgc ggcgatcgcg gtgggctgta tcgccgcatt cgtcgtactc
      481 aagtacgtca gcggtgtcga ggaacgagcc aacggggacg ccgagcgcgt ctcggtcctg
      541 gtcgttcgag cggacatccc ccagggagtc cccggtgagc aggccgtgca ggagggttgg
      601 atcgtcgagg acgagatcgc ccgcgagttc ctgcccgcca cccgcatcac cgacttcgac
      661 cagatcaccg ggaaggtggc cctgaacacg ctggccgcca accaggtcct ggtggagggg
      721 atgttcgtcg atccgacgac caactcgatc gggttcggac gacgcctcga ggagaaccac
      781 gtcgcctaca cgatctcggt ggacagcacg agcggcgtcg ccggcctgct cgtccccggt
      841 gacctggtcg acatcttcgc cacgaccggt gagggcgagc ccaccgacgc ggaggtgtcg
      901 tccgacacgg ccgagcagca ggggatcttc gggcagaacg cccgcctcct ctaccaccag
      961 gtccgcatcc tggccatcgg cacggagacc gcgccgcagc ccggtgagga acaggccgcc
     1021 gccgagggtg aggacgcggc cgccgaagcg ccgcaggcca gcggcctgct caccctcgat
     1081 ctcccgatcg acgccgccca gctcatcgcc tcggtggact ccggttccct gcgcctggcc
     1141 ctgaccgggc cggactacca ggctcagccg atcccccgga tcgacctcaa cgacctggcc
     1201 gagctgcccg gcgagaccga cgcccgaacc cgcttcggga cgctcacccc ctacgggccc
     1261 gacggccccc agtaaccgac ccacccgccg aatcgcccca ccggagcctg agctatgagc
     1321 aacctgttct ctgaccccct cggcagcacc gactcgttcg gtggcgccgc gggcgtcgag
     1381 ctggcgccca tcggggtgtc ggtgccgacg ggctacccgg gcggcgacgc cttcggtggc
     1441 agcaacggca ccatgggtct cgagcctctg tcctcggccc cggacctgag ccacctgccg
     1501 ctggccaccg tcgacgtcga cgagatggtc cgcacccagc tggcgatgca gctcggcacc
     1561 cacgccgtgc ccttcccgtc gatcgaggcg ctggcgacca agctcaccgg gacgcctctg
     1621 gtcgtggtcc tgggcccgtc gatggcctcg ccggaccagt tggtcgtggt cgaacgcctg
     1681 acccgccctc ggcccgagat cggggccatc ctcatcgccg agcagctcac cacggaggtc
     1741 ctccagcagg cccttcgggc cggcgtccgg gacgtgctgc cgctccagga cctctcccag
     1801 ctggccatga cggtcaagcg ggtcggcgac atgctcggca gtggctcgca gcagccggcg
     1861 atcatggacc cgaacaacgt ccacggagcc cccgagcccg gcggcgacgg ccaggtcatc
     1921 accgtcttct cgaccaaggg tggggcgggc aagtcggtca tcgcctgcaa cgtggccgtg
     1981 cgcctggcca agaagagcaa ccggcccgtg gccctggtcg acgccgacct ccagttcggc
     2041 gacgtggccg tcatgctcaa gctggccccc cagcacacca tcgtcgacgc cgtcggctcg
     2101 ctcgatcgcg gcatggacgc ggcgatgctc cagagcctgc tcgtgcagca cgatgcctcc
     2161 ggcctctacg tgctgcccgc ccccctggag ccggccttcg ccgaccagat ccggggcgag
     2221 gagatggtgc gcatcgtcga ggtcctgcgc agcttctgca gtcacgtcat catcgacacg
     2281 ccggcgtact tcaacgacgt cgtcctcggc ctgatcgaga tcagcgacga cgtgctcctg
     2341 gtcgccggga tggacatccc gaacatcaag aacgtgaaga tcgggctgca gaccctgcgc
     2401 ctgctcaaca cgccgatgtc caagctccgc ctggtcctca accgggccaa ctccaaggtg
     2461 aagctggacg tcagcgaggt cgagaagacc ctgcaggtca aggccgatgc cctgatcccg
     2521 agcgacgtcg tggtgcccca gtcggtgaac aagggcatcc ccgtggtcct cgccgccccc
     2581 aaggcggccg tcacccgggc catcgaacaa ctcgccgaca ccttcctgat tgccgagacc
     2641 gccggtcgtt gaccggtaac caaccgccga ggagaagctg atgtcgctgt acaagcgcct
     2701 gcaggacggg ggaggagccc ccgcgggtca gagcggcctg cgggaccctg tgctcgacga
     2761 gctgcggcac aagatccacc acgtcctcat cgacgagctg ggccccatcc tctacgacaa
     2821 gcggatctcc gaggaggacc tgcggcgtcg ggtccacgag cagctccacg ccgccctggc
     2881 ccaggagcgg gcgccgctgt cggccgccga caaggcccag ctcatccagg acgtctccga
     2941 cgacatcctc ggctacggac ccatcgaccg gctgctcaac gacgaggagc tgaccgaggt
     3001 catggtcaac ggaccgagct cggtctacat cgagcgctcc ggcaagctcg agaagaccga
     3061 cgcccgcttc gtggacgagg agcacctccg ccgcatcatc gacaagatcg tcagtgaggt
     3121 cggtcgtcgc ctggacgagt cgagccccat ggtggacgcc cgtctgcccg acggctcccg
     3181 cgtcaacgcg gtgatccacc cgctggccat cggtgggccc ttcctcacca tccgaaagtt
     3241 ctccaaggac ccgttccaga tcgacgacct gatccgcttc gggaccctga acgccgcctc
     3301 ggcccgcttc ctgcaggcct gcgtggtcgg gcgcctgaac ctgatcgtcg ccggcggtac
     3361 cggtaccggc aagaccacga ccttgaacgt gctctcctcc ttcatccccg ccgacgagcg
     3421 catcgtcacc atcgaggacg ccaaggagct ccagctgcac caggaccacg tcctggcgtt
     3481 ggaggcccgc ccgcccaaca tcgagggtcg gggccaggtc accatccgtg acctcgtcaa
     3541 gaacgccctg cgcatgcgtc ccgaccggat cgtggtcggg gagtgtcgtg ccggcgaggc
     3601 cctggacatg ctccaggcca tgaacacggg ccatgacggc tcgatcacga ccatccactc
     3661 caacacgcct cgggacaccc tgtcccgtat cgagacgatg accctgatgg ccggcttcga
     3721 cctgccggtc cgggccatcc gggagcagat ggcctccgcc ctggactgca tcgtgcagct
     3781 gacccgactg cgggacggca cccggcgcat cacccacgtg accgaagtgc agggcatgga
     3841 gggcgacgtc atcaccctcc aggacgtgtt cctgttcgat tacggcgccg gcgtggacga
     3901 gcgcgggaag ttccggggcc acctcaaggc cacgggcgtt cgccccaagt tcaccgagaa
     3961 gctccaggac ctgggcatcc gcctcggccc cgaggtcttc cagcccgagc cctccggcgg
     4021 acaccagatg atcggctccg cccgatgagg atcctgcgcg ccgcgctcgg cggcctgctc
     4081 accgcggccg tcctggccgg accggccatc gccttcgcgg ccgacgacga ggccgccgcg
     4141 ccgtacctgg ccgtccgtgg cgtcgactc
//
LOCUS       DASTFV010000121         6219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_420038, whole genome shotgun sequence.
ACCESSION   DASTFV010000121 DASTFV010000000
VERSION     DASTFV010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420038"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /gene="rpsS"
                     /locus_tag="VFP02_02215"
     CDS             <1..195
                     /gene="rpsS"
                     /locus_tag="VFP02_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET9541867.1"
                     /translation="KVIQTWSRRSTIIPEMVGHTIAVHDGRKHVPVYITESMVGHKLG
                     EFAPTRTFRAHAGQERAGRR"
     gene            195..548
                     /gene="rplV"
                     /locus_tag="VFP02_02220"
                     /pseudo
     CDS             195..548
                     /gene="rplV"
                     /locus_tag="VFP02_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
     gene            1013..1648
                     /gene="rpsC"
                     /locus_tag="VFP02_02225"
                     /pseudo
     CDS             1013..1648
                     /gene="rpsC"
                     /locus_tag="VFP02_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            1960..2376
                     /gene="rplP"
                     /locus_tag="VFP02_02230"
     CDS             1960..2376
                     /gene="rplP"
                     /locus_tag="VFP02_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET9541868.1"
                     /translation="MLMPKKVKHRKQQRGHRRGPAKGGTTVNFGDYGIQALEPGWITA
                     RQIEAARIAMTRHIKRGGKVWINIFPDKPVTQKPAETRMGSGKGNPEYWVAVVKPGRI
                     LFELSYPDESTAKAALERAIQKLPIKARFVRREEAF"
     gene            2376..2600
                     /gene="rpmC"
                     /locus_tag="VFP02_02235"
     CDS             2376..2600
                     /gene="rpmC"
                     /locus_tag="VFP02_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006235039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET9541869.1"
                     /translation="MKAAQVRELGDADLVQQLDDARQELFNLRFSHVTGQLDNATALR
                     DTRREVARLMTELRAREIRAAEALEETTDA"
     gene            2593..2874
                     /gene="rpsQ"
                     /locus_tag="VFP02_02240"
     CDS             2593..2874
                     /gene="rpsQ"
                     /locus_tag="VFP02_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET9541870.1"
                     /translation="MPETETTESTERNRRKTREGMVVGTKMDKTAIVAVTDRVRHRRY
                     DKTVQRVTKLYVHDEDNTLNVGDRVKVAETRPLSKNKRWRLLEIVERAR"
     gene            2871..3239
                     /gene="rplN"
                     /locus_tag="VFP02_02245"
     CDS             2871..3239
                     /gene="rplN"
                     /locus_tag="VFP02_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET9541871.1"
                     /translation="MIQQETRLRVADNSGAREVLCIKVLGGSRRRYATIGDVFVATVK
                     DAIPGAAVKKGEVVRCVVVRVKKEKRRPDGSYIRFDENAAVLINDQQQPRGTRIFGPV
                     GRELRDKRFMRIVSLAPEVL"
     gene            3251..3556
                     /gene="rplX"
                     /locus_tag="VFP02_02250"
     CDS             3251..3556
                     /gene="rplX"
                     /locus_tag="VFP02_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET9541872.1"
                     /translation="MKIKKGDRVVVLTGKDKGKEGEVSRAIPSAGKVIVDGVNVAKRH
                     QKATRATMQGGIIDKDMPIPVANVAILCPSCGATRVGYRFDPDGTKYRTCKKCGSDL"
     gene            3556..4122
                     /gene="rplE"
                     /locus_tag="VFP02_02255"
     CDS             3556..4122
                     /gene="rplE"
                     /locus_tag="VFP02_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET9541873.1"
                     /translation="MAATSTKPRLQQRYDDEIRQKLKDDLGLGNIMEVPRLTKIVLNC
                     GVGAATDQKSLIDGAVADLTIIAGQKPVVTKATKSIANFKLREGNPIGAKVTLRGARM
                     WEFFDRLINLAIPRIRDFRGLPDRSFDGHGNYTFGVTEQLIFPEIDYDAIDTVRGMDI
                     TIVTTARTDAEGRALLDAYGFPFRRNED"
     gene            4126..4311
                     /locus_tag="VFP02_02260"
     CDS             4126..4311
                     /locus_tag="VFP02_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HET9541874.1"
                     /translation="MAKKALVEKANKTPKFKVRGYTRCQRCGRSRAVYRKFKLCRLCL
                     RELAHAGELPGMTKASW"
     gene            4316..4720
                     /gene="rpsH"
                     /locus_tag="VFP02_02265"
     CDS             4316..4720
                     /gene="rpsH"
                     /locus_tag="VFP02_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET9541875.1"
                     /translation="MTMTDPIADMLTRIRNANVAMHDDVTMPSSKLKESLAAVLQKEG
                     YIQAFRVDQEEGKPSKTLTIDMKYSEARERVITGIRRVSKPGLRVYTKADAVPRVLGG
                     LGVAVVSTSKGLLTDREARRQRIGGEILCYVW"
     gene            4729..5274
                     /gene="rplF"
                     /locus_tag="VFP02_02270"
     CDS             4729..5274
                     /gene="rplF"
                     /locus_tag="VFP02_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET9541876.1"
                     /translation="MSRVGQAPIPVPSGVEVKIEGRVVTVKGPKGELSRTLPEVLSIT
                     QDAGELTVDRADEERESRALHGLFRSLVANMVTGVTEGYRRGLEIVGVGYRATAQGDR
                     ALELAVGYSHTVKVQAPEGITFEVPSNTRIDVVGIDKETVGQVAADIRAIRKPEPYKG
                     KGIRYQGEVVRRKAGKAASAG"
     gene            5291..5653
                     /gene="rplR"
                     /locus_tag="VFP02_02275"
     CDS             5291..5653
                     /gene="rplR"
                     /locus_tag="VFP02_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET9541877.1"
                     /translation="MAPTAKKATARQRRHGRVRKKVQGTADRPRLAVFRSNRHISVQV
                     IDDRAGVTLAAASTYETELKGSATGNKDAAAKVGERIAERAKAAGVTSVVFDRGGNLY
                     HGRVAAVADAAREGGLEF"
     gene            5653..>6219
                     /gene="rpsE"
                     /locus_tag="VFP02_02280"
     CDS             5653..>6219
                     /gene="rpsE"
                     /locus_tag="VFP02_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET9541878.1"
                     /translation="MSSYQREQAPNPRASGDPQLRESRVIDINRVAKVVKGGRRFSFT
                     ALVVIGDANGRVGLGYGKAKEVPLAIQKGTEEARRNLFKVPMAGSTIVHPILGEWGAG
                     RVLLKPAAPGTGVIAGGAARAILEEAGIHDVLCKSLGSANHNNVARATLQGLKGQRRP
                     DAIAALRGIPAEEFVPKGLLEAYLATEKG"
BASE COUNT         1128 a         2234 c         2088 g          769 t
ORIGIN      
        1 aaggtcatcc agacctggtc tcgccggtcc acgatcatcc cggagatggt gggtcacacc
       61 atcgccgtcc acgacggtcg caagcacgtg cccgtctaca tcaccgagtc catggtcggt
      121 cacaagctgg gcgagttcgc tcccacgcgc accttccgcg cccacgccgg ccaggaacgg
      181 gcgggtcgac gctgatggct gcgcccgccg cggtcaagga ccgtccgcac acccaggctc
      241 aggtgcgcta cgtgcgcatg tcggcctcga aggcccgcca ggtcctggat ctgatccggg
      301 gcaagtccgt cggtgccgcc gacgacgtcc tggccctctc cgagcgcgac gccgcgctgg
      361 tcatccagaa gtgcctgcgc tcggccgtgg ccaacgccga gcacaacgac ggcctcgacc
      421 ccgaggagct cttcgtggcg acctgtttcg ccgacgaggg cccgacgctg cgccgcttcc
      481 gcccccgggc ccgcggccgc gccacccgga tccgcaagcg gacctgccac atcacggtga
      541 tcgtggcccg ctactcgccc gacgagctgg ccgtgcgccg cgagatcgag cggaccaagg
      601 gcagcggcaa gggccgcagc tccgacgcct ccagccgccg tcgccgggtg gccaagagcc
      661 ggggtgagga ggtcccgacc gaggacgccg tcgaggagac cgtcgacgag ccgacggtcg
      721 acgagaccga cacggccgtc gaggcgaccg ccgaggagct cgtcgaagaa cacaccaagg
      781 atgagctgct cgagatcgcc gacgaggccg gggtcgaggt ggccaagagc tcgaacaagg
      841 ccgagatcgc cggggccatc gaggtggcga cggcgtcggc cgacgagctg gccgaggccc
      901 acacgaagga cgagctgctg gagctggccg agcaggccgg catcgacgtg gcgaagagct
      961 ggacgaagcc ccagatcgcc gaggcgatcg tggcttcacg agaggacgac tgatgggaca
     1021 gaaggtcaac ccttacgggt tccgactcgg ggtcaccacc gactggaagt cgcgctggtt
     1081 cgcggaccgc gaggagtacg ccgacttcat catcgaggac tggaagatcc gggactacct
     1141 gatgggcgag ctgccccacg ccgccatcag ccgggtcgag gtcgagcgca cgcgtgaccg
     1201 cctgcgcatc gacgtccaca ccgcccgccc cggcatcgtc atcggtcgcc gcggcgccga
     1261 ggccgaccgc ctgcgggccg ggctgacgaa gatcaccggc aacgccaagg tccagctcaa
     1321 catccaggag atcaagcagc ccgagctcga cgccgcgctg atcgcccagg gtgtcgccga
     1381 ccagctcgcc ggccgggtcg ccttccgccg ggccatgaag cgggccgtgc agaacgccca
     1441 gaaggccggt gcccagggca tccgggtcca gtgctcgggt cgcctcggcg gctccgagat
     1501 ggcccgcacc gagtggtacc gcgagggtcg ggtccccctg cacaccctgc gggccgacat
     1561 cgactacggc ctgcgtgagg cccacaccac cgccggccgc atcggcgtga aggtctggat
     1621 ctacaagggc gacatcctcc cctacaagcc cgccaacgag gagaagagcg tccgtgaggc
     1681 ggccatggcc gtcggcgaga cctccggcca ggccgcgcct gagccccggg tcgtgtcctc
     1741 caccgccgcc cgcaagcgcg aagaggagaa ggcccagaag gaagcggccg ccgcggctgc
     1801 cgccgaggcc gatgccgagc cgaccgagga cgcgccggag cccctcatca aggaggccga
     1861 tcccgagttc gagcgcctcc tcgccgagga agaggagatc gagcggacca cccgcgagca
     1921 ccacgagacg ccgcacttcc gcccgggaga cgctgactga tgctgatgcc caagaaggtc
     1981 aagcaccgca agcagcagcg cggtcaccgg cgcggcccgg ccaagggcgg caccaccgtc
     2041 aacttcggcg actacggcat ccaggccctc gagcccgggt ggatcaccgc ccgccagatc
     2101 gaggccgccc gaatcgccat gacccgccac atcaagcgtg gtggcaaggt ctggatcaac
     2161 atcttcccgg acaagcccgt gacccagaag ccggccgaga cccgcatggg ctcgggcaag
     2221 ggcaaccccg agtactgggt cgccgtggtc aagccgggcc ggatcctctt cgagctgtcc
     2281 taccccgacg agagcaccgc caaggcggcc ctcgagcggg ccatccagaa gctaccgatc
     2341 aaggcccgct tcgtacgccg cgaggaggcg ttctgatgaa ggccgcccaa gtccgtgagc
     2401 tcggcgacgc cgacctcgtc cagcagctgg acgacgcccg ccaggagctc ttcaacctcc
     2461 gcttctccca tgtcaccggc cagttggaca acgccacggc gctgcgggac acccgccgcg
     2521 aggtggcccg gctcatgacc gagctgcggg cccgagagat ccgcgccgcc gaggcgctcg
     2581 aggagaccac cgatgcctga gaccgagacc accgagtcga cggagcgcaa ccggcgcaag
     2641 acccgcgagg gcatggtcgt gggcacgaag atggacaaga ccgccatcgt ggccgtcacc
     2701 gaccgcgtcc ggcaccgtcg ctacgacaag accgtccagc gggtcaccaa gctctacgtc
     2761 cacgacgagg acaacaccct caacgtgggt gaccgggtca aggtggccga gacccgcccg
     2821 ctgtccaaga acaagcgttg gcgcctgctc gagatcgtgg agcgggcccg atgatccagc
     2881 aggagacccg gctccgggtc gccgacaaca gcggggcccg tgaggtcctg tgcatcaagg
     2941 tgctcggcgg ctcccgccgg cgctacgcga ccatcggcga cgtgttcgtg gccacggtca
     3001 aggacgccat tcccggtgcg gccgtcaaga agggcgaggt cgtccggtgc gtcgtcgtcc
     3061 gggtcaagaa ggagaagcgt cgtccggacg gctcctacat ccgcttcgac gagaacgccg
     3121 ccgttctcat caacgaccag cagcaacccc gcggcacccg catcttcggg cccgtgggcc
     3181 gcgagctgcg ggacaagcgc ttcatgcgca tcgtctccct cgccccggag gtgctctgac
     3241 catggcaggc atgaagatca agaagggcga ccgcgtcgtc gtcctcaccg gcaaggacaa
     3301 gggcaaggag ggcgaggtct cccgcgccat cccctcggcc ggcaaggtga tcgtcgacgg
     3361 cgtgaacgtg gccaagcgtc accaaaaggc gacccgggcc accatgcagg gcgggatcat
     3421 cgacaaggac atgcccatcc cggtggccaa cgtcgccatc ctctgcccca gctgcggcgc
     3481 cacccgggtc ggctaccgct tcgaccccga cggcaccaag taccggacgt gcaagaagtg
     3541 cgggagtgac ctgtgatggc cgccaccagc accaagccgc gcctgcagca gcgctacgac
     3601 gacgagatcc gccagaagct gaaggacgac ctcgggctgg ggaacatcat ggaggtcccc
     3661 cgcctgacca agatcgtcct caactgcggc gtcggggcgg cgaccgacca gaagtcgctc
     3721 atcgacggcg ccgtcgccga cctcacgatc atcgccggcc agaagccggt ggtgaccaag
     3781 gccaccaagt ccatcgccaa cttcaagttg cgcgagggca accccatcgg ggccaaggtg
     3841 accctgcggg gcgcccgcat gtgggagttc ttcgatcggc tgatcaacct ggccattccc
     3901 cgcatccggg acttccgggg gctgcccgac cggtcgttcg acggtcacgg caactacacg
     3961 ttcggcgtga ccgagcagct gatcttcccc gagatcgact acgacgcgat cgacaccgtc
     4021 cgaggcatgg acatcacgat cgtcaccacc gcccgcaccg acgccgaagg ccgagccctg
     4081 ctcgacgcct acggcttccc cttccgacgc aacgaggact gagccatggc caagaaggca
     4141 ttggtcgaga aagccaacaa gaccccgaag ttcaaggtcc gcggatacac ccgctgccag
     4201 cggtgtggcc gctcgcgggc ggtgtaccgc aagttcaagc tgtgccgcct gtgcctgcgg
     4261 gagctggcgc acgcgggcga gctgcccggc atgacgaagg cgagttggtg atccgatgac
     4321 gatgaccgac ccgatcgccg acatgctcac gcgcatccgc aacgccaacg tggcgatgca
     4381 cgacgacgtg acgatgccca gctccaagct caaggagagc ctggccgccg tcctccagaa
     4441 ggagggctac atccaggcct tccgtgtcga ccaggaggag ggcaagccct ccaagaccct
     4501 gaccatcgac atgaagtact ccgaggcccg cgagcgggtg atcaccggca tccgccgcgt
     4561 gtccaagccc ggcctgcgcg tctacaccaa ggccgacgcc gtcccccgcg ttctcggggg
     4621 cctgggggtc gccgtcgtct cgaccagcaa gggactcctc accgaccgcg aggctcgccg
     4681 ccagcgcatc ggtggcgaga tcctctgcta cgtctggtag gagccgagat gtcgcgagtt
     4741 ggacaagcac ccatcccggt cccctccggg gtcgaggtca agatcgaggg ccgcgtggtc
     4801 accgtgaagg gccccaaggg cgagctgtcg cgcaccctgc ccgaggtcct cagcatcacc
     4861 caggacgcag gcgagctgac ggtggaccgc gccgacgagg agcgggagag ccgggccctg
     4921 cacggcctct tccggtcgct ggtggccaac atggtcacgg gcgtgaccga gggctaccgc
     4981 cgcggcctcg agatcgtggg ggtcggctac cgggccaccg cccagggcga tcgtgccctg
     5041 gagctggccg tgggctacag ccacacggtc aaggtccagg cccccgaggg gatcaccttc
     5101 gaggtgccct ccaacacccg catcgacgtc gtcggcatcg acaaggagac ggtcggccag
     5161 gtggccgccg acatccgcgc cattcgcaag cccgagccgt acaagggcaa gggcatccgc
     5221 taccagggag aggtcgtccg ccgcaaggcc ggcaaggccg cttccgcagg ctgagtacga
     5281 aggaatcgtc atggcaccca ccgccaagaa ggccacggcc cgccagcgcc gacacggtcg
     5341 cgtccgcaag aaggtccagg gcacggccga ccggcctcgc ctggccgtct tccggtcgaa
     5401 ccggcacatc agcgtgcagg tcatcgacga ccgcgccggg gtcaccctcg ccgcggcgtc
     5461 gacctacgag accgagctca agggctcggc gaccgggaac aaggacgccg cagccaaggt
     5521 cggcgagcgc atcgccgagc gggccaaggc cgccggcgtc acctctgtgg tcttcgaccg
     5581 cggcggaaac ctctaccacg ggcgcgtcgc cgccgtggcc gatgccgccc gtgagggcgg
     5641 actggagttc tgatgagcag ctaccagcgt gaacaggccc ccaacccgcg ggccagtggg
     5701 gacccgcagc tgcgggagtc ccgggtcatc gacatcaacc gcgtggccaa ggtggtcaag
     5761 ggcgggcggc ggttctcctt caccgccctc gtggtcatcg gtgacgccaa tggccgggtc
     5821 ggcctgggct acggcaaggc caaggaggtg cccctggcca tccagaaggg caccgaggag
     5881 gcccgccgca acctgttcaa ggttcccatg gcgggcagca cgatcgtgca ccccatcctc
     5941 ggcgagtggg gcgctgggcg tgtcctgctg aagcccgccg ctcccggtac cggggtgatc
     6001 gccggcggcg ccgcccgggc gatcctcgaa gaggccggca tccacgacgt gctctgcaag
     6061 tcgctgggct cggccaacca caacaacgtc gcccgggcca ccctccaggg cctgaagggc
     6121 cagcgtcgtc ccgacgccat cgccgcgctg cggggcatcc ccgcggagga gttcgtgccc
     6181 aagggcctgc tcgaggcgta cctggccacc gagaagggt
//
LOCUS       DASTFV010000122         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_422520, whole genome shotgun sequence.
ACCESSION   DASTFV010000122 DASTFV010000000
VERSION     DASTFV010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422520"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VFP02_02285"
     CDS             complement(<1..530)
                     /locus_tag="VFP02_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9541879.1"
                     /translation="MIRVVVADDQALVRAGLRVLIDAEPEVEVVGEAEDGEAAVALVA
                     ETRPDVVLMDIRMPKLDGLAATRQIVSDATLDGVRVLILTTFELDEYVFEALRSGASG
                     FLVKDTEPVELLRAIRVVAEGESLLSPGVTRRLIGEFVAQPQGRTFAADALEPLTDRE
                     REVVAQVAAGLSNDEIA"
     gene            complement(527..1771)
                     /locus_tag="VFP02_02290"
     CDS             complement(527..1771)
                     /locus_tag="VFP02_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9541880.1"
                     /translation="MSVPALLHRQTVQDALISVVYTVSVVAITVRLDHGGEPGRPLDV
                     LAYVCMIGVGVALAFRRRWPTGTLYAILALTLVYVIRDYTGGPFFLAVFIAIATVASV
                     MPTREALPRVAIAFVALALSGIFVDSADESGWVHLLYLSWSVVAFLAGKTVRDRRELL
                     TGLRERNRHLEETQEEEARRRVAEERVRIARDLHDIVAHNIAAISLQAATGAYVADAH
                     PDKARDALLAIRQASRQTLDDLRCTLDVLRDGDDSAPLAPTPGLSALPMLVDSVVEAG
                     VPVTLVLEGDTGAVPEAVAGAAYRIVQESLTNVMRHAGPARVTVAVTRLADGLDVSVE
                     DDGRGASAAPGPTGHGLAGMRERALTFGGTFAAGPRPGGGFAVRAWLPVVLEPDEPCA
                     DSSGRPDHAPIPHPAGAGGASS"
BASE COUNT          302 a          715 c          743 g          241 t
ORIGIN      
        1 gcgatctcgt cgttgctcag gccggcggcg acctgggcca cgacctcccg ctcccggtcg
       61 gtgagcggct ccagggcgtc ggcggcgaac gtccggccct ggggctgggc cacgaactcg
      121 ccgatgaggc gccgcgtgac gccgggcgac agcagggact cgccctcggc cacgacccgg
      181 atcgcccgca gcagctcgac cggttcggtg tccttgacca ggaagccgct ggcccccgac
      241 cgcagggcct cgaagacgta ttcgtcgagc tcgaaggtgg tcaggatcag gacccggacc
      301 ccgtcgaggg tcgcgtcgga aacgatctga cgggtggcgg ccaggccgtc gagcttcggc
      361 atccggatgt ccatgaggac cacgtcgggc cgggtctccg ccaccagggc cacagcggcc
      421 tcgccgtcct cggcctcgcc cacgacctcg acttcgggct cggcgtcgat caggacccgg
      481 aggccggccc gcaccagcgc ctggtcgtcg gccaccacga cgcggatcac gagctggccc
      541 cgccggcacc cgccggatga gggatggggg cgtggtcggg ccgaccggac gagtctgcac
      601 acggctcatc gggctcgagg acgaccggca gccaggcccg cacggcaaag ccgccgcccg
      661 gcctcggccc cgcggcgaac gtcccgccga acgtgagggc ccgctcccgc atgccggcca
      721 acccatggcc ggtcggccca ggggccgcgg aggcgcctcg accgtcgtcc tccacggaga
      781 cgtcgaggcc atcggcgagg cgggtcacgg cgaccgtgac ccgagcgggg ccggcgtggc
      841 gcatgacgtt ggtcagcgac tcctggacga tgcggtaggc cgccccggcg acggcctcgg
      901 gcacggcgcc ggtgtcgccc tcgagcacca acgtcacggg cacgcccgcc tccacgacgg
      961 agtcgacgag catgggcagc gccgagaggc cgggcgtggg cgccagcggc gccgagtcat
     1021 cgccgtcccg caggacgtcg agggtgcagc ggaggtcgtc gagggtctgg cggctggcct
     1081 ggcgaatggc cagcagggcg tcgcgggcct tgtcgggatg ggcgtcggcc acataggcac
     1141 cggtcgcggc ctgcaggctg atggcggcga tgttgtgggc cacgatgtcg tgcaggtccc
     1201 gagcgatgcg gacccgttcc tcggccacgc ggcgccgggc ctcttcctcc tgggtctcct
     1261 caaggtgtcg gttgcgctcc cgcaggccgg tgaggagctc gcggcggtcc cgaaccgtct
     1321 tgccggccag gaaggccacg accgaccagc tcaagtagag caggtggacc caacccgact
     1381 cgtcggcgct gtccacgaag atcccgctga gggccagggc cacgaacgcg atggccaccc
     1441 gtggaagggc ctcgcgggtg ggcatgacgc tggccacggt cgcgatggcg atgaacacgg
     1501 ccaggaagaa gggcccgccg gtgtagtcac ggatcacgta gaccagggtc agggccagga
     1561 tggcgtacag cgtccccgtc ggccagcggc gacggaacgc cagggccacg ccgacgccga
     1621 tcatgcacac gtaggccagg acgtcgaggg gacgccccgg ctccccgccg tggtcgaggc
     1681 ggaccgtgat ggcgacgacc gacaccgtgt agacgacgct gatcagcgcg tcctggaccg
     1741 tctgacgatg cagcagggcc gggacgctca cccccgcgac gttacggggg gctggctcct
     1801 gcgccatccc gtggacgacg taactgcggg ctacgtccct tgacgtacgt cccggcgcga
     1861 cgggcgtcgg gcccacagcc cgcccgtgac caggagcgac cacaggctga ccgcggtcag
     1921 cacgatcagg aaggcgacat agccgtaggc cgcggcctca tcccaggcca gttcgtcgcc
     1981 gtagcggagg agggcgacgc c
//
LOCUS       DASTFV010000123         2377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_430632, whole genome shotgun sequence.
ACCESSION   DASTFV010000123 DASTFV010000000
VERSION     DASTFV010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430632"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..391
                     /locus_tag="VFP02_02295"
     CDS             41..391
                     /locus_tag="VFP02_02295"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET9541881.1"
                     /translation="MSKPVGAYRPIVRAGEWLVVSGQIGLNDGALVGGGFRDEVVQAL
                     DNLEGLLAGEGATLAQVVKTTVYLRHMGDYGILNEEWVGRFGDSLPARAAVAVSELPL
                     HALVEIEAWAYTGA"
     gene            405..560
                     /locus_tag="VFP02_02300"
     CDS             405..560
                     /locus_tag="VFP02_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541882.1"
                     /translation="MAGPVLIVVALLLLPVLMCMGGAVLAAILGWSVKANVDAANEGS
                     ELLELNT"
     gene            complement(575..1282)
                     /locus_tag="VFP02_02305"
     CDS             complement(575..1282)
                     /locus_tag="VFP02_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004105653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9541883.1"
                     /translation="MSAADTAAATEPVLVFPDPAPADLVQTLDLSGFAWKAVGDEVGA
                     TRNEPDSGWGGAVVCADTDPEGAFALCRALRRRDAPVAPVLLLISGAQLGELELREDL
                     YDDFCLSPFHPRELEARLRHQFWRSGGGVQAELVEYEPLVLNLETYQALIAGRPLDLT
                     YMEYELLKFLAQHPGKVFTRETLLSRVWGYEYYGGARTVDVHVRRLRAKLGEEHANLI
                     QTVRSVGYRFGQSRWAG"
     gene            complement(1303..>2377)
                     /locus_tag="VFP02_02310"
     CDS             complement(1303..>2377)
                     /locus_tag="VFP02_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET9541884.1"
                     /translation="EDLEGNPFEGDPRGILRRNLDRARERGFSFFCAPELEFFYFADG
                     DASTPLQPLDQGSYFDLTTADVASDLRKRTIHTLEAMSIPVEYSFHEDSPSQHEIDLR
                     YDDALSMADSVMTARLVVREIALERGVYATFMPKPLEGVQGSGMHTHLSLFEGDVNAF
                     HDAGDELGLSKVGRHFIAGLLHHAPEITAITNQLVNSYKRLVQGTGTGSQTVFEAPVF
                     VSWGRNNQSALVRVPVVKRGKESSTRIEYRAPDPACNPYLAYSVLLAAGMKGVEEGYD
                     LPEPQQGNIYELTAEERAAEGLSALPQSLSEALDAMEGSELVAEALGEHVHDWFLRNK
                     RNEWAAYKAAVTPFELERYLPVW"
BASE COUNT          346 a          781 c          890 g          360 t
ORIGIN      
        1 ccaccggcca ccgctaccgc tgagggctac cgtcctcgcc atgtcgaagc ctgtgggtgc
       61 ctaccgaccg atcgtccgag ccggggagtg gctggtcgtc tccgggcaga tcgggctgaa
      121 cgacggcgcg ctcgtcgggg gcgggttccg ggacgaggtc gtccaggcgc tggacaacct
      181 ggagggcctg ctcgccggtg agggggccac cctggcccag gtggtgaaga cgacggtcta
      241 cctccgccac atgggtgact acgggatcct caacgaggag tgggtgggtc gcttcggcga
      301 cagcctcccg gcccgggctg cggtggcggt cagcgaactg cccctccacg ccctggtcga
      361 gatcgaggcc tgggcctaca ccggcgccta gctaaggttc ggcgatggct ggaccggtgc
      421 tgatcgtcgt cgccttgctg ctcctgccgg tgctgatgtg catgggtggc gccgtcctgg
      481 ccgccatcct cggctggtcc gtcaaggcca acgtggacgc ggccaacgag ggctccgagc
      541 tcctcgagct caacacgtag cgagtggctc gacctcagcc ggcccagcgg gactggccga
      601 agcggtagcc caccgagcgc acggtctgga tcaggttggc gtgctcctcg cccagcttgg
      661 cccgcaggcg ccggacgtgg acgtcgaccg tgcgggcccc gccgtagtac tcgtagcccc
      721 agacccggct gagcagggtc tcccgggtga agaccttgcc cgggtgttgg gccaggaact
      781 tcagcagctc gtactccatg taggtgaggt cgagcgggcg accggcgatg agggcctggt
      841 aggtctcgag gttgaggacc agaggctcgt actccacgag ctcggcctgg acaccgccac
      901 ccgagcgcca gaactggtgg cgcaggcggg cctccagctc gcgggggtgg aagggcgaga
      961 ggcagaagtc gtcgtagagg tcctctcgca gctccagctc gcccagctgg gcgccggaga
     1021 tgagcaggag gacgggggcc accggcgcgt cccggcggcg aagggcgcgg cagagggcga
     1081 aggccccctc ggggtcggtg tcggcgcaga ccacggcgcc accccaaccg ctgtcgggct
     1141 cgttgcgggt ggccccgacc tcgtcgccca cggccttcca ggcgaacccg gacaggtcca
     1201 gggtctggac caggtcggcc ggggcggggt cagggaagac caggaccggt tcggtcgcgg
     1261 cggcggtgtc cgcggcgctc atccccatca gcctgccacg cgtcaccaga cggggaggta
     1321 acgctcgagc tcgaacgggg tgacggcggc cttgtaggcg gcccactcgt tgcgcttgtt
     1381 gcgcaggaac cagtcgtgga cgtgctcgcc cagggcctcg gccacgagct cggagccctc
     1441 catcgcgtcg agggcctcgg agagcgactg ggggagggcg ctcaggccct cggccgcccg
     1501 ctcctccgcg gtgagctcgt agatgttgcc ctgctggggc tcgggcaggt cgtaaccctc
     1561 ctcgaccccc ttcatgccgg ccgccaacag gaccgagtac gccaggtagg ggttgcaggc
     1621 gggatcgggg gcccggtact cgatgcgggt cgaggactcc ttgccccgct tgaccaccgg
     1681 cacccggacg agggcggact ggttgttgcg cccccacgag acgaacaccg gggcctcgaa
     1741 caccgtctgg gagccggtgc cggtgccctg caccaggcgc ttgtaggagt tcaccagctg
     1801 gttggtgatg gcggtgatct cgggggcgtg gtgcagcagg ccggcgatga agtggcggcc
     1861 gaccttggac aggcccagct cgtcgccggc gtcgtggaag gcgttgacgt caccctcgaa
     1921 gagcgagagg tgggtgtgca tgcccgagcc ctgcacccct tcgagcggct tcggcatgaa
     1981 ggtggcgtac acgccccgct ccagggcgat ctcacgcacg acgagccggg cggtcatcac
     2041 gctgtcggcc atcgacaggg cgtcgtcgta gcgcaggtcg atctcgtgct ggctggggct
     2101 gtcctcgtgg aagctgtact ccacggggat gctcatggcc tccagcgtgt ggatggtccg
     2161 cttgcgcagg tccgaggcca cgtcggcggt ggtcaggtcg aagtacgagc cctggtcgag
     2221 cggctgcaac ggcgtcgagg cgtcgccatc ggcgaagtag aagaactcca gctcgggggc
     2281 acagaagaag gagaagcccc gctcgcgggc ccggtccagg ttgcggcgca ggatgccccg
     2341 ggggtcgccc tcgaagggat tgccctccag gtcctcg
//
LOCUS       DASTFV010000124         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_437996, whole genome shotgun sequence.
ACCESSION   DASTFV010000124 DASTFV010000000
VERSION     DASTFV010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437996"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VFP02_02315"
     CDS             <1..1191
                     /locus_tag="VFP02_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9541885.1"
                     /translation="TPTLNWYINPDNGGQSRLAEKCVDASNGAYQVDIQTLPNSASDQ
                     REQLVRRLAAQDSSIDVMSLDPPFVAEFANAGFLRPFSKEDEATFTDGVLKGPLETTY
                     WNDQLVAAPFWANTQLLWFRKSAAEAAGVDPTEPGFTWDQMIKAAESQQKVIGVQANR
                     YEGYMVWINALVVSAGGEIIGNVEAGKDATPEINSPAGDAAAEIVGGLARSPAAPPAM
                     STAGEEESRSVFQGDSGMFMVNWPYVYSAAQGDAEQSVVDDISWARYPGVEAGEASRP
                     PLGGINLAIGAYTKYPEQALDLVKCATSLQSNIEYMVDSGNPAAKRAAYDDPKVREAF
                     PMASLIRDSIDEAGPRPVTPYYNDVSTSVQRVWHPATEVRAPQTPEETATFMTDVLQG
                     KRLL"
     gene            1352..>2113
                     /locus_tag="VFP02_02320"
     CDS             1352..>2113
                     /locus_tag="VFP02_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9541886.1"
                     /translation="MTAYPLIYAVVLAFYDYRLTDPGGRTFVGLSNFATVLTDPVWWS
                     DFTTTLIITVVSVGVELVLGFAFAMVMYKILFGRSLIRTGILVPYGIITVVSAFIWRY
                     AFQLDSGFVNQWFGLGDFNWFGERWSSLTVITLSEIWKTTPFISLLLLAGLVQVPAEL
                     NKAAMVDGATAWQRLMRVTLPNMKAAIMVALLFRTLDAWRIFDNPFIMTAGANDTETL
                     SILAYRQNVTLLNLGVGSAISVLLFFTVVIIALIFI"
BASE COUNT          346 a          714 c          694 g          359 t
ORIGIN      
        1 accccgacct tgaactggta catcaacccg gacaacggcg ggcagagccg cctggccgag
       61 aaatgcgtcg acgcctccaa cggtgcctac caggtcgaca tccagacgct gccgaactcg
      121 gcgtccgacc agcgggagca gctggtccgg cggctggccg cccaggacag ctcgatcgac
      181 gtgatgagcc tggacccgcc gttcgtcgcc gagttcgcca acgccggctt cctccgtccg
      241 ttctccaagg aggacgaggc caccttcacc gacggcgtcc tcaagggccc gctggagacg
      301 acctactgga acgaccagct ggtggcggcc ccgttctggg ccaacaccca gctgctctgg
      361 ttccgcaagt cggccgcgga ggcggccggc gtcgacccca ccgaaccggg cttcacctgg
      421 gaccagatga tcaaggcggc cgagagccag cagaaggtga tcggggtgca ggccaaccgg
      481 tacgagggct acatggtctg gatcaacgcc ctcgtggtgt cggccggggg cgagatcatc
      541 ggcaacgtgg aagccggcaa ggacgccacc cccgagatca actccccggc gggagatgcg
      601 gcggccgaga tcgtcggcgg cctcgccagg tccccggcgg cgccaccggc gatgtccaca
      661 gccggtgagg aggagtcccg ttcggtcttc cagggtgact cgggcatgtt catggtcaac
      721 tggccgtacg tctattccgc ggcccagggc gacgccgagc agtcggtcgt ggacgacatc
      781 agctgggccc gataccccgg ggtcgaggcg ggcgaggcga gccgtccgcc gctgggcggg
      841 atcaacctgg ccatcggcgc ctacaccaag tacccggagc aggcgctcga cctggtcaag
      901 tgtgccacct cgctgcagag caacatcgag tacatggtgg actccggcaa cccggcggcg
      961 aagcgggccg cctacgacga ccccaaggtc cgcgaggcct tcccgatggc ctcgctgatc
     1021 cgtgactcga tcgacgaggc cgggccgcgg ccggtcacgc cctactacaa cgacgtctcg
     1081 acgtcggtgc agcgggtctg gcatccggcg acggaggtcc gggctccgca gacgccggag
     1141 gagacggcga ccttcatgac cgacgtcctg cagggcaagc ggctcctgtg agccgaccgg
     1201 cgcctcgccc cgagaggagc accccgtgac cgttaccgaa ccccgcgtcg aggccccggc
     1261 gcaaccgccg cccgaagtgt ccgagcggac gcggtacgaa cggcggctgg gcctggccct
     1321 gtcagccccg gcattcatcg tcatggtctt cgtgaccgcg tacccgttga tctacgcggt
     1381 cgtcctcgcc ttctacgact accggttgac cgaccccgga ggaaggacct tcgtcgggct
     1441 cagcaacttc gcgacggtgc tgaccgaccc cgtgtggtgg tccgacttca cgaccacgct
     1501 gatcatcacc gtggtgagcg tgggtgtgga gctggtgctc ggcttcgcct tcgccatggt
     1561 gatgtacaag atcctgttcg gccgctcctt gatccgcaca gggatcctcg ttccgtacgg
     1621 catcatcacg gtcgtgtcgg cgttcatctg gcgctatgcg ttccagctcg actccggatt
     1681 cgtgaaccag tggttcggcc tcggcgattt caactggttc ggtgagcgct ggtcgtcgct
     1741 gactgtcatc acgttgtcgg agatctggaa gacgacgccg ttcatctcgt tgctcctgct
     1801 ggccggcctg gtgcaggtgc ccgccgagct gaacaaggcg gccatggtcg acggcgccac
     1861 ggcgtggcag cggttgatgc gggtgacgtt gcccaacatg aaggccgcga tcatggtggc
     1921 cttgttgttc cggaccttgg acgcgtggcg gatcttcgac aatccgttca tcatgacggc
     1981 gggggccaac gacaccgaga cgctgtcaat cctggcctac cgtcagaatg tgaccctgct
     2041 caacctgggc gttggatcgg cgatctcggt actgctcttc ttcaccgtgg tcatcatcgc
     2101 cctcatcttc atc
//
LOCUS       DASTFV010000125         3356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_442257, whole genome shotgun sequence.
ACCESSION   DASTFV010000125 DASTFV010000000
VERSION     DASTFV010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442257"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2311)
                     /locus_tag="VFP02_02325"
     CDS             complement(<1..2311)
                     /locus_tag="VFP02_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9541887.1"
                     /translation="MVVIVWVLALFVLNGVAGGVGSAFRDDLNLPDVESRDGFDLLDE
                     QFGGQGTGYTGTIVFRAEQGVDDPEVQEQMQELFDLVAGLDDVARVDSPYAEGGEELI
                     SSQGPEAGLIAYANVELPEESDFTRAAEIRDTILEQSPEIDGLTIELGGFIFADFEEP
                     TAELLGLAFAIVILIVAFGSVLAMGLPIGVALVGIGLGSALTTLLSHVITIPEFAPFI
                     GVMIGLGVGIDYALLIITRYREQLHAGQDVRTSISIAMDTAGRSVLFAGMTVVISLLG
                     MLLMGVPFVQGLAVSASVTVALTVAASLTLLPALIGFAGERIELTRRRGLIAAGFLAL
                     GLVGAGLGFSPLLIGFPIAVAVLLLGIWVPYLKAEVPHRAPKPRRETVAYRWSRIIQH
                     RPWPAALVGTAILLVLAAPVLGLRLGFSDESNFPEDTTTRQAYDLLVDGFGEGFNGPF
                     LLVADVGEGADVEALGAVTDAVAADPEVVFVSPPVPNDPENPTAVLWNVVPSSGPQEE
                     ATTELVNRLRDDVLPPAEDATGTEVLVTGATPINVDFAEFLQGRLPYFFGAVLVLSFL
                     LLMVVFRSLLVPLKAVIMNLLSIGAAYGVMVALFQWGWLSDLTGVAQAPVEPWMPMML
                     FAIVFGLSMDYEVFLLSRIREEYNRTGDSHTSVADGLAATAKVITAAAAIMVVVFGSF
                     VLESDRTFKLMGTGLATAIFLDATIVRMLLVPATMELLGDRNWWLPRWLDRILPNVDI
                     EGHSELAVHAGDDDLDEDDPRRDADLVGVD"
     gene            2523..3200
                     /locus_tag="VFP02_02330"
     CDS             2523..3200
                     /locus_tag="VFP02_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9541888.1"
                     /translation="MRILVVEDEAPMATSIQRGLVAEGFAVDIATDGDEGLWMTREFP
                     YDLIVLDVMLPGTNGYVVCRTLREEGSRVPILMLTAKRGDLDEAEGLETGADDYLTKP
                     FSFVVLVARVRALLRRAQGERAPRLQAGDLVLDAADRTVRRGEVTIDLRNREFAVLEY
                     LLRHAGEVVSKAEIVEHVWDLNFDGDLNIVEVYVSSLRKKIDGPFDRHALETVRGIGY
                     RLRIDGG"
BASE COUNT          555 a         1161 c         1182 g          458 t
ORIGIN      
        1 agtcgacgcc gaccaggtcg gcgtcgcgcc gcgggtcgtc ctcgtcgaga tcgtcgtcgc
       61 cggcgtggac ggcgagctcg gagtggccct cgatgtcgac gttgggcagg atccggtcca
      121 gccacctcgg cagccaccag ttccggtcac ccagcagttc catcgtcgcc gggaccagga
      181 gcatccgcac gatcgtggcg tcgaggaaga tggccgtggc cagcccggtg cccatcagct
      241 tgaacgtgcg gtcggactcg agcacgaagc tgccgaagac cacgaccatg atcgccgccg
      301 ccgcggtgat gaccttggcg gtggcggcca agccgtcggc caccgacgtg tggctgtccc
      361 ccgtccggtt gtactcctcc cgtatccggg agaggaggaa cacctcgtag tccatggaca
      421 ggccgaagac gatggcgaac agcatcatcg gcatccaggg ctcgacgggg gcctgggcga
      481 ccccggtgag gtcgctgagc cagccccact ggaacagggc caccatcacc ccgtaggccg
      541 ccccgatcga cagcaggttc atgatcacgg ccttcagcgg gaccagcagg gaccggaaga
      601 ccaccatcag gagcaggaag gacagcacca gcacggcgcc gaagaagtac ggcaggcggc
      661 cctggaggaa ctcggcgaag tccacgttga tcggcgtcgc cccggtgacc agcacctcgg
      721 tgccggtggc gtcctcggcc ggggggagga cgtcgtcgcg gagacgattg accagttcgg
      781 tggtggcctc ctcctggggg cccgaggagg gcaccacgtt ccagaggacc gcggtggggt
      841 tctccgggtc gttgggcacc ggcggcgaga cgaagacgac ctccgggtcg gccgccactg
      901 cgtcggtgac ggcgccgagc gcctcgacgt cggcgccctc gccgacgtcg gccacgagca
      961 ggaacgggcc gttgaagccc tcaccgaagc cgtcgaccag caggtcgtag gcctgccggg
     1021 tggtggtgtc ctcggggaag ttgctctcgt cggagaagcc gaggcgcagc ccgaggacgg
     1081 gcgcggccag caccaacagg atcgccgtcc cgaccagagc cgcaggccac ggccgatgct
     1141 ggatgatccg gctccagcgg taggccaccg tctcccgccg tggcttcggc gcgcggtggg
     1201 gcacctccgc cttcaggtac ggcacccaga tgcccagcag cagcaccgcc accgcgatcg
     1261 ggaagccgat gagcaggggt gagaacccga gtccggcccc caccaggccg agcgccagga
     1321 agccggcggc gatcaggccc cggcggcggg tgagctcgat gcgctcgccc gcgaaaccga
     1381 tgagggcggg gagcagcgtg agtgaggccg cgaccgtgag ggcgacggtg accgaggcgc
     1441 tcacggccag gccctggacg aagggcacgc ccatcagcag catgcccagc aacgagatga
     1501 ccacggtcat ccccgcgaag aggaccgatc gccccgcggt gtccatcgcg atgctgatcg
     1561 acgtccgtac gtcctggccg gcgtggagct gttctcgata ccgggtgatg atgagcaggg
     1621 cgtagtcgat gcccacgccg aggccgatca tgacgccgat gaacggggcg aactcgggga
     1681 tcgttatgac gtggctgagc aaggtcgtca gggccgagcc gagaccgatg ccgacgaggg
     1741 ccacaccgat gggcaggccc atggccagca ccgacccgaa ggcgacgatc aggatgacga
     1801 tggcgaacgc caggcccagc agctcggcgg tcggctcctc gaagtcggcg aagatgaacc
     1861 cgcccagctc gatggtcagg ccgtcgatct cgggcgactg ctccaggatc gtgtcccgga
     1921 tctccgcggc gcgggtgaag tcactctcct cgggcagctc gacgttggcg taggcgatca
     1981 ggcccgcctc cggcccctgg gaggagatga gctcctcgcc gccctcggcg taggggctgt
     2041 cgacccgggc cacgtcgtcg aggccggcga ccaggtcgaa gagctcctgc atctgctcct
     2101 ggacctcggg atcgtccacg ccctgttcgg cccggaagac gatcgtgccc gtgtacccgg
     2161 ttccctggcc cccgaactgc tcgtcgagca ggtcgaagcc gtcgcgggac tccacgtcgg
     2221 ggaggttgag gtcatcgcgg aacgcgctgc cgacaccgcc cgcgacgccg ttcaggacga
     2281 acagggcgag gacccacacg atgacgacca accagcggcg gtcatgacac caggggccga
     2341 gacgagcgaa cacagagacc tccggggggt ggcgaacgac gactcttcct acccgcttcg
     2401 actcggcaac ccaaagaaat tcatcggtgc cggcgtcgcg acctcgcgat cacccgggcg
     2461 ctcggcgtca tccgcggccc cgcctcccgg cggcgccggc ccgggccgta gcgtgaggcg
     2521 cgatgcggat cctggtggtc gaggacgagg ccccgatggc cacgtcgatt cagcgggggc
     2581 tggtggccga gggcttcgcc gtcgacatcg cgacggatgg cgacgagggc ctctggatga
     2641 cccgggagtt cccctatgac ctgatcgtcc tcgacgtgat gctgcccggg acgaacggct
     2701 acgtggtctg ccgcacgctg cgagaggagg gcagccgggt ccccatcctc atgctcacgg
     2761 ccaagcgggg ggacctcgac gaggccgagg gcctggagac cggggccgac gactacctca
     2821 ccaagccctt ctcattcgtg gtcctcgtgg cccgtgtccg ggccctgctg cgtcgggccc
     2881 agggcgaacg cgcccctcgc ctccaggccg gggacctcgt cctggacgcc gccgaccgca
     2941 cggtgcgccg gggcgaggtc accatcgacc tgcgcaaccg ggagttcgcc gtcctcgaat
     3001 acctcctgcg ccacgccggc gaggtggtgt ccaaggccga gatcgtcgag cacgtctggg
     3061 acctgaactt cgacggcgac ttgaacatcg tcgaggtcta cgtcagctcg ctgcgcaaga
     3121 agatcgacgg acccttcgac cggcacgccc tcgagaccgt ccgggggatc ggctaccggc
     3181 tgcggatcga cggcggatga gccccgtccg ctggcgctcg gtgcgcctgc gcatggtgct
     3241 ggccgccgtc gccgtgctcg ccgtcctgct cgtcactgcc gccatcggct tcgacctcct
     3301 cctgcgtagc cggctcatcg acaacctgga cgcctcgttg caggcccaga cgaccg
//
LOCUS       DASTFV010000126         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_442570, whole genome shotgun sequence.
ACCESSION   DASTFV010000126 DASTFV010000000
VERSION     DASTFV010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442570"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /locus_tag="VFP02_02335"
     CDS             complement(<1..1359)
                     /locus_tag="VFP02_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET9541889.1"
                     /translation="MLDVNNFHQLTISLATADSIRTWSNGEVKKPETINYRTLKPEKD
                     GLFCEKIFGPTKDWECYCGKYKRVRFKGIICERCGVEVTRSKVRRERMGHIELAAPVV
                     HIWYLRGTRSWLAYLLTGTEPKDELKAKQLEKVIYFAANLVWMVDDEKRHAELPNLEV
                     ELAGEKEAIEKDRELEIAQRLEELEAEVAELEGEGAKANDLKSRRRAADKDLEEIRER
                     YEEEMDLVQRAFDEFRDLFPRKIIEDEVLWREMADRYGEYFEGGMGADAIARLLEQLD
                     LDAEEETLRSQIDPPEGQKPLSAQRKQKAIKRLKIVSAFNRRDEHGRRSNDPRAMILD
                     VVPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPEIIVNNEKRM
                     LQEAVDALFDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIV
                     VGP"
     gene            complement(1392..>2717)
                     /locus_tag="VFP02_02340"
     CDS             complement(1392..>2717)
                     /locus_tag="VFP02_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET9541890.1"
                     /translation="GPEEITRDIPNLSDDILADLDERGIVRVGAEVGPGDVLVGKVTP
                     KGETELTPEERLLRAIFGEKAREVRDTSLKVPHGEQGKVIDVKLFSRDEQHELPPGVN
                     QLVRVYVGQKRKISVGDKLAGRHGNKGVISKILPLEDMPFMADGSPIDIILNPLGVPS
                     RMNVGQVLEAHLGYVARWGWDIDGDGKADVGDEPIRGTETKTRPTTKPATLIATPVFD
                     GAHWETADVVDRANDHPTIKELLTNIVPESVDGERLVQTTGKAQLYDGRTGEPYDNPV
                     MVGQIYILKLAHLVDDKIHARSTGPYSMITQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYCLQELLTIKSDDVLGRVKVYEAIVKGENIPEPGIPESFKVLIKEMQALCINVEVLS
                     QAGEEIEMRELDEDVFRTAEELGIDISRPERGTDEDDELRAASRTTGRI"
BASE COUNT          346 a          893 c          956 g          522 t
ORIGIN      
        1 cgggccgacg acgatgaccg aacggccgga gtagtcgacg cgcttgccga gcaggttctg
       61 gcggaaccgg ccctgcttgc ccttgagcat gtcggacagg gacttgaggg gccggttgcc
      121 ggggcccgtg accgggcggc cgcggcggcc gttgtcgaac agggcgtcga cggcctcctg
      181 cagcatccgc ttctcgttgt tgacgatgat ctcgggggca ccgaggtcca gcagtcgctt
      241 gaggcggttg ttccggttga tgacccggcg gtacaggtcg ttgaggtccg acgtggcgaa
      301 gcggccaccg tcgagctgga ccatcgggcg cagctccggc gggatgaccg ggacgacgtc
      361 gaggatcatg gcccgcgggt cgttggagcg ccggccgtgc tcgtcgcggc ggttgaaggc
      421 cgacacgatc ttcaggcgct tgatggcctt ctgcttgcgc tgggccgaga ggggcttctg
      481 gccctcgggg gggtcgatct ggctgcggag ggtctcctcc tcggcgtcca ggtcgagctg
      541 ctcgagcagg cgggcgatgg cgtcggcgcc catgccgccc tcgaagtact cgccgtagcg
      601 atcggccatc tcccgccaga ggacctcgtc ctcgatgatc ttgcggggga agaggtcgcg
      661 gaactcgtcg aaggcgcgct ggacgaggtc catctcctcc tcgtagcgct cgcggatctc
      721 ctcgaggtcc ttgtccgcgg cccggcggcg ggacttgagg tcgttggcct tggccccctc
      781 gccctccagc tcggccacct cggcctcgag ctcctccagg cgctgggcga tctccagctc
      841 ccggtccttc tcgatggcct ccttctcgcc ggccagctcg acctcgaggt tgggcagctc
      901 ggcgtggcgc ttctcgtcgt ccaccatcca gacgaggttg gccgcgaagt agatgacctt
      961 ctccagctgc ttggccttca gctcgtcctt gggctcggtg cccgtgagca ggtaggccag
     1021 ccaggagcgg gtgccccgca ggtaccagat gtggacgacg ggggcggcca gctcgatgtg
     1081 gcccatgcgc tcgcgccgga ccttggagcg ggtcacctcg acgccgcagc gctcacagat
     1141 gatgcccttg aagcggacgc gcttgtactt gccgcagtag cactcccagt ccttggtcgg
     1201 cccgaagatc ttctcgcaga agaggccgtc cttctcgggc ttcagcgtgc ggtagttgat
     1261 cgtctccggc ttcttgacct caccgttgga ccacgtgcgg atcgagtccg cggtggccag
     1321 ggagatcgtg agctggtgga agttgttgac gtcgagcatg cgtgttggtt cctcttcctc
     1381 gatgtccggt tctagatccg gccggtcgtg cggctggccg cccggagttc gtcgtcttcg
     1441 tcggtgcccc gctcgggccg gctgatgtcg atgccgagct cctcggccgt gcggaacacg
     1501 tcctcgtcca gctcccgcat ctcgatctcc tcgcccgcct gggagaggac ctcgacgttg
     1561 atgcagaggg cctgcatctc cttgatgagg accttgaagc tctcggggat gcccggctcg
     1621 gggatgttct cgcccttgac gatggcctcg tagaccttca cccggccgag cacgtcgtcg
     1681 gacttgatgg tcaggagctc ctgcaggcag taggcggcgc cgtaggcctc gagggcccag
     1741 acctccatct ccccgaagcg ctggccaccg aactgggcct taccgcccag cggctgctgg
     1801 gtgatcatgg agtacgggcc ggtggaccgg gcgtggatct tgtcgtccac caggtgggcc
     1861 agcttcaaga tgtagatctg gccgaccatg accgggttgt cgtagggctc gccggtgcga
     1921 ccgtcgtaga gctgggcctt gcccgtggtc tggaccaggc gctcgccgtc gaccgactcc
     1981 gggacgatgt tggtcaggag ctccttgatc gtcgggtggt cgttggcccg gtcgaccacg
     2041 tccgcggtct cccagtgggc gccgtcgaac accggcgtgg cgatgagcgt cgccggcttg
     2101 gtggtcggac gggtcttggt ctcggtgccc cggatgggct cgtcgccgac gtcggccttg
     2161 ccgtcgccgt cgatgtccca accccaacgg gcgacgtagc cgaggtgcgc ttcgagcacc
     2221 tggcccacgt tcatccggga ggggacgccg agggggttca ggatgatgtc gatgggcgag
     2281 ccgtcggcca tgaacggcat gtcctcgagg ggcaggatct tggagatcac gcccttgttg
     2341 ccgtggcggc cggccagctt gtcgcccacg ctgatcttgc gcttctggcc gacgtagacc
     2401 cgcaccagct ggttcacgcc gggcggcagc tcgtgctgct cgtcccggct gaagagcttg
     2461 acgtcgatga ccttgccctg ctcgccgtgg ggcaccttca gggaggtgtc ccgcacctca
     2521 cgggccttct ccccgaagat ggcccgcagg agccgctcct cgggggtgag ctcggtctcg
     2581 cccttgggcg tgaccttgcc gaccaggacg tcgccgggac ccacctcggc cccgacccgc
     2641 acgatgccgc gctcgtcgag gtcggccagg atgtcgtcgg agaggttcgg gatgtcccgg
     2701 gtgatctcct ccgggcc
//
LOCUS       DASTFV010000127         5662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_443545, whole genome shotgun sequence.
ACCESSION   DASTFV010000127 DASTFV010000000
VERSION     DASTFV010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443545"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFP02_02345"
     CDS             complement(<1..306)
                     /locus_tag="VFP02_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541891.1"
                     /translation="MIWAVAPYPLLLSPLDIGPVTLRNRVVMGAHFTMFTEPTAAGEP
                     GFYGERTGRYLADRARGGVGAVICGQAAVHPTTAYQMPNNAAAWLPEAVPHFRRVADQ
                     "
     gene            405..1790
                     /locus_tag="VFP02_02350"
     CDS             405..1790
                     /locus_tag="VFP02_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9541892.1"
                     /translation="MKRRRVLFAVITVGALVAAGCGGDRGEDASDTASTETTEPAGGG
                     AGDFGDLEGVCGPNEGGGELSTAGPEETQGITADTITLGTVSDPGFEGRPGLNQEIFD
                     AGTAFVEWCNAAGGINGKQLELNLHDAAINEYQPVMAQSCETDFAIVGSGAVQDNFWP
                     ETGAACGLIDIAGFSVTAEKAGLAGRDAIEARSVQPVPNAGDRFQVGSYVTVDAENPD
                     AAARSGIIYGDLDTIIAQKDKVIDGLEQIGHTFVHEAAYNILGEANWAPFAQALQDDD
                     VRFLHFVGEGEFFALLVQAMDEIGYRPDLMLMETNLYDQNWVDAAGPAADGVYVRTVF
                     WPFEEAADNPATQQYIDVVNAVDGKVALLGAQSMSSWLLFAQAARDCDLDDDLTRSCV
                     LEGAASATDWTGGGLHAPTNPGSNEPTPCTIVLQIQDGGFSRFAPEEGYDCGDESDQP
                     YVVDVEATGVS"
     gene            1859..3241
                     /locus_tag="VFP02_02355"
     CDS             1859..3241
                     /locus_tag="VFP02_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9541893.1"
                     /translation="MKARRVLLAVVTVGVLLAAGCGSDRGEDESGGTDSSTTESTAPA
                     EAAAGDFGDLTGVCGPNEGGGAVSDAGPDETQGITADGITVGTVSDPGFEGLPGLNQE
                     IFDAGSAFVEWCNAAGGINGKQLTLNLRDAAVTEYQPVLEQSCQEDFGIVGSGAVQDN
                     FWPEVGASCGLIDIAGFSVTPDKAGLAGRDPIEARSVQPVPNPSDRYQVGSYLIIDAE
                     YPDAPARTGILYGDIETTAIQAERVQAGLEAIGHTIVHTAAYNILGEANWAPFAAALQ
                     EDDVQWLQFVGSGDNLALLLQAMDEIGYRPEVIAQDANFYDQTWIDAAGPSADGVFVR
                     TVFNPFEEAADNPAVQQYIDHVEAIDGKVASLGTQAWSAWLLFAQAARDCDLDDNLTR
                     SCVLEGASSVTDWTGGGLHVPTSPGTNEPPECTLVLQIEDSTFNRWAPDEGFDCGEDS
                     DGPFVVDINI"
     gene            3344..4723
                     /locus_tag="VFP02_02360"
     CDS             3344..4723
                     /locus_tag="VFP02_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9541894.1"
                     /translation="MTIRRLFALLTVLGLVAAGCSGDRGEDESGGVSDESTESTASAV
                     AAGDFGDLTGVCGPNEGGGEVSGDPAETQGITADSITVGTVADPGFEGRPGLNQELFD
                     AGTAFVEWCNAAGGINGKEVVLNLRDAAVTEYQPVLEQSCQEDFGIVGSGAVQDNFWP
                     TTGAECGLIDVAGFSVTPEKAGSAGDDPIATRSVQPVPNPLNEFPVGAASILNDEFPD
                     AGANTGILYADLDTLITQAARTTEAYESLGMNVVYESTYNLLGEANWAPFAAAVQQAG
                     VTFLTFVGEGANLALFQQALDEIGYDVEVTLQDANFYDQSYIDAAGAAAEGTYVRNAF
                     WPLEEAADNPATQQYIDHVEAVDGKVALLGAQAWSAWLLFAQAARDCDLDGDLTRTCV
                     LDGAASVTEWTGGGLHAPTNPAENRGPDCTLVLQVQDGAFVRHAPTDGDYDCDPAYVV
                     QLEGDYAVG"
     gene            4778..>5662
                     /locus_tag="VFP02_02365"
     CDS             4778..>5662
                     /locus_tag="VFP02_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9541895.1"
                     /translation="MDQFLTFTVFGLVTAAIYAVAASGLVVTYTTSGIFNFAHGAFGM
                     LAAFMYWRLRVEWGWPAPISLLIMLCLVAPLFGATIERVIFRGLQDVSEVTKLVVSVS
                     LLFGVYQGAIILFPPDQGRRFPGFYDGNAFDLGVVNLQWHDAFTIVAAILVAVFLRFL
                     LFGTRTGIAMRAVVDDRPLAQLTGSRPDRSAMLAWAIGTSLAALSGILLAGSQGVLKH
                     LDLTLLVINAYAAAMIGRLRSLPLTFVGALILGLGESYAVGYLPTNQEITSIFGWNDF
                     TPISLSGLRPAFPVILLFI"
BASE COUNT          854 a         2012 c         1952 g          844 t
ORIGIN      
        1 ctggtcggcc acccggcgga agtggggaac cgcctccggc agccacgccg ccgcgttgtt
       61 gggcatctgg tacgccgtcg tcgggtggac cgcggcctgg ccgcagatga cggcgccgac
      121 cccgccccga gcccggtcgg ccagataccg ccccgtccgc tcgccgtaga accccggctc
      181 gcccgcggcg gtgggctcgg tgaacatcgt gaagtgcgcc cccatgacca cccggttgcg
      241 cagcgtcacg ggaccgatgt cgagggggga gagcaggagc gggtacgggg ccacggccca
      301 gatcatggcg cgatcccccg ggggtcgacc gaaaggcgcg tgcgatccgc gcgatagtgg
      361 tcgaggcgac cgaccggggg gtcgggacca catcggggga accaatgaag cgacgtcgcg
      421 tcctgttcgc cgtgatcacc gtcggggcgc tggtggccgc cggctgtggt ggagatcggg
      481 gcgaggacgc ctccgacacc gcgtcgaccg agaccaccga gcccgccggt ggcggggcgg
      541 gcgacttcgg tgacctcgaa ggggtgtgcg ggcccaacga gggcggcggg gagctgtcga
      601 ccgcgggacc cgaggagacg cagggcatca ccgccgacac catcacgctg ggcaccgtgt
      661 ccgaccccgg cttcgagggc cgtcccgggc tcaaccagga gatcttcgac gccggcaccg
      721 ccttcgtcga gtggtgcaac gccgccgggg gcatcaacgg caagcagctc gagctcaacc
      781 tgcacgacgc ggcaatcaac gagtaccagc cggtcatggc ccagtcctgc gagaccgact
      841 tcgccatcgt gggctcgggc gcggtgcagg acaacttctg gccggagacc ggtgccgcct
      901 gtggcctcat cgacatcgcc ggcttctccg tcacggccga gaaggcgggc ctggccggcc
      961 gggacgcgat cgaggcccgg tccgtgcagc ccgtgcccaa cgccggagac cgcttccagg
     1021 tgggctcgta cgtgacggtc gacgccgaga atccggacgc cgcggcccgc tccggcatca
     1081 tctacggcga cctcgacacg atcatcgccc agaaggacaa ggtgatcgac ggcctcgagc
     1141 agatcggtca caccttcgtg cacgaggccg cctacaacat cctgggcgaa gccaactggg
     1201 cccccttcgc ccaggccctg caggacgacg acgtccggtt cctccacttc gtcggcgagg
     1261 gggagttctt cgccctcctc gtccaggcca tggacgagat cggctaccgc ccggacctga
     1321 tgctcatgga gaccaacctc tacgaccaga actgggtcga cgccgcgggg ccggcggcgg
     1381 acggggtcta cgtgcggacg gtcttctggc ccttcgagga agcggcggac aacccggcga
     1441 cgcagcagta catcgacgtg gtcaacgcgg tcgacggcaa ggtcgccctc ctcggggccc
     1501 agtcgatgtc gtcgtggctg ctgttcgccc aggcggcccg cgattgcgat ctcgacgacg
     1561 acctgacccg gagctgtgtc ctggagggcg cggcgtcggc cacggactgg accggcggcg
     1621 ggctccacgc ccccacgaac ccggggtcga acgagccgac gccgtgcacg atcgtcctgc
     1681 agatccagga cggaggcttc agccgtttcg ccccggagga gggctacgac tgcggggacg
     1741 agagcgacca gccctacgtc gtggacgtcg aggcgacggg cgtttcctga cgggtcgtca
     1801 gatccgcgcg atagtggtgc cgatcgccga cccgggggcc ggcgcacaag gggggaacat
     1861 gaaggcacgt cgggtcctgc tcgcggtcgt gaccgtgggc gtcctgctcg ctgcaggttg
     1921 tggcagtgat cgcggcgagg acgagtcggg gggaaccgac agctccacga ccgagtcgac
     1981 cgcgcccgcg gaggcggccg ccggtgactt cggtgacctg accggcgtgt gcggccccaa
     2041 cgaagggggc ggggcggtgt ccgacgccgg gccggacgag acccagggga tcaccgccga
     2101 cgggatcacg gtcggcaccg tgtccgatcc cggcttcgag ggtcttcccg gcttgaacca
     2161 ggagatcttc gacgccggct ccgctttcgt cgagtggtgc aacgccgcgg gtggcatcaa
     2221 cggcaagcag ctgaccctga acctgcgcga cgcagccgtc accgagtacc agccggtcct
     2281 cgagcagtcg tgccaggagg acttcggcat cgtcggctcc ggggccgtgc aggacaactt
     2341 ctggcccgag gtcggtgcgt cctgcggtct gatcgacatc gccgggttct cggtcacccc
     2401 cgacaaggcc ggcctggccg ggcgggatcc gatcgaggcc cgctcggtgc agccggtgcc
     2461 caacccgtcg gatcggtacc aggtcggctc gtacctcatc atcgacgccg agtaccccga
     2521 cgctccggcc cgcaccggga tcctctacgg cgacatcgag accacggcca tccaggccga
     2581 gcgggtccag gccgggctgg aggcgatcgg tcacacgatc gtgcacacgg cggcgtacaa
     2641 catcctgggc gaggcgaact gggcgccgtt cgcggccgcg ctgcaggagg acgatgtgca
     2701 gtggctgcag ttcgtcggct cgggtgacaa ccttgctctg ctcctccagg ccatggacga
     2761 gatcggctac cgccctgaag tcatcgccca ggacgccaac ttctacgacc agacctggat
     2821 cgacgccgcc ggcccgtcgg ccgacggggt gttcgtccgc accgtgttca acccgttcga
     2881 ggaggccgcc gacaacccgg cggtccagca gtacatcgac cacgtcgaag ccatcgacgg
     2941 caaggtggcc tcgctcggga cccaggcctg gtcggcgtgg ctcctgttcg cccaggcggc
     3001 gcgggactgt gacctggacg acaacctgac ccgcagctgc gtcctcgagg gggcgtcgtc
     3061 ggtgacggac tggaccggtg gggggctgca cgtgcccacc agcccgggca ccaacgagcc
     3121 gccggagtgc accctcgtcc tgcagatcga ggacagcacc ttcaaccgtt gggcccccga
     3181 cgagggcttc gactgcggtg aggacagcga cgggccgttc gtggtcgaca tcaacatctg
     3241 accccgaatc tgacgatccg tcagtttgtg cttagatggc ccctcgcccg gcggggggcc
     3301 atctcgcgcc gtggccgccc tgtttccaca cctgagggga tccatgacca tccgccgcct
     3361 gttcgcgctg ctcactgtgc tcggactcgt cgctgccggc tgcagcggtg atcgggggga
     3421 ggacgagtcc ggcggcgtga gcgacgagtc gaccgagtcg accgccagcg cggtggcggc
     3481 cggtgacttc ggcgacctga ccggggtgtg tggcccgaac gaggggggcg gtgaggtcag
     3541 cggcgacccc gccgagaccc agggcatcac ggccgacagc atcaccgtgg gcacggtggc
     3601 cgatcctggg ttcgagggtc ggcccggcct gaaccaggag ctcttcgacg ccggcacggc
     3661 cttcgtcgag tggtgcaacg ccgccggtgg gatcaacggc aaggaggtcg tgctgaacct
     3721 gcgcgacgcg gcggtgaccg agtaccagcc ggtgctggag cagtcgtgcc aagaggactt
     3781 cgggatcgtc ggctccgggg cggtgcagga caacttctgg cccaccaccg gcgccgagtg
     3841 cgggctgatc gacgtcgccg gcttctcggt caccccggag aaggcgggga gcgccggtga
     3901 cgacccgatc gccacacgat cggtccagcc cgtgcccaat ccgctcaacg agttccccgt
     3961 cggcgcggcg tcgatcctga acgacgagtt ccctgacgcc ggggccaaca ccggcatcct
     4021 ctacgccgac ctggacaccc tcatcaccca ggcggcccgg acgacggagg cctacgagag
     4081 cctcggcatg aacgtggtct acgagagcac ctacaacctc ctcggcgagg ccaactgggc
     4141 cccgttcgcc gccgcggtgc agcaggccgg cgtgaccttc ctcaccttcg tgggcgaggg
     4201 cgccaacctg gccctgttcc agcaggcgct ggacgagatc ggctacgacg tcgaggtcac
     4261 gcttcaggac gccaacttct acgaccagtc ctacatcgac gccgccggcg ccgcggccga
     4321 gggcacctac gtccgcaacg ccttctggcc cctcgaggaa gcggccgaca acccggccac
     4381 ccagcagtac atcgaccacg tcgaagccgt ggacggcaag gtcgctctgc tcggcgccca
     4441 ggcctggtcg gcgtggctgc tgttcgccca agccgcccgg gactgtgacc tcgacggcga
     4501 cctgacccgg acctgcgtgc tcgacggcgc cgcctcggtg accgagtgga ccggcggagg
     4561 gctgcacgcc cctacgaacc cggccgagaa ccggggcccg gactgcacgc tcgtcctcca
     4621 ggtccaggac ggggccttcg tccgccacgc acccaccgac ggggactacg actgcgatcc
     4681 ggcctatgtg gtccaactcg agggggacta cgccgtcggt tagcgccgcg gcgacatcgt
     4741 aagctcagcg cccgatcgcc ctggggggta cgtcggtttg gaccagttcc tgacgttcac
     4801 ggtcttcggg ctcgtgacgg ccgccatcta tgccgtcgcg gccagcggcc tcgtggtgac
     4861 ctacaccacc tcgggcatct tcaacttcgc ccacggcgcg ttcgggatgc tggccgcctt
     4921 catgtactgg cggctccggg tcgagtgggg ttggccggcc cccatctcgc tcctcatcat
     4981 gctctgcctg gtcgccccgc tgttcggcgc caccatcgag cgggtcatct tccggggcct
     5041 ccaggacgtg tccgaggtga ccaagctggt cgtctcggtc agcctcctct tcggggtcta
     5101 ccagggcgcc atcatcctct tcccgccgga ccagggccgg cggttccccg ggttctacga
     5161 cggcaacgcc ttcgacctcg gcgtggtcaa cctccagtgg catgacgcct tcaccatcgt
     5221 ggccgccatc ctcgtggccg tcttcctgcg cttcctgctg ttcgggaccc gcaccggcat
     5281 cgccatgcgg gcggtggtcg acgaccgccc gctggctcag ctcaccggga gccgccccga
     5341 ccggtcggcc atgttggcct gggccatcgg gacgtcgctg gccgccctgt ccgggatcct
     5401 gctggccggc agccagggtg tgctcaagca cctggacctg accctgctgg tcatcaacgc
     5461 ctacgccgcg gccatgatcg gccggctgcg cagcctgcct ctcaccttcg tgggcgccct
     5521 gatcctgggc ctcggcgagt cctatgccgt gggctacctg cccacgaacc aggagatcac
     5581 ctcgatcttc ggctggaacg acttcacgcc gatctccctc agcgggctcc gcccggcctt
     5641 cccggtgatc ctcctcttca tc
//
LOCUS       DASTFV010000128         3796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_456109, whole genome shotgun sequence.
ACCESSION   DASTFV010000128 DASTFV010000000
VERSION     DASTFV010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456109"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VFP02_02370"
     CDS             <1..631
                     /locus_tag="VFP02_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9541896.1"
                     /translation="GRRPLMALRIDNVITSGIFSLDGEDFEVDNNIWLVGDDDEVIVV
                     DAAHDHRPIVAGVGSRRVVAVVCTHGHNDHINAAVPLAEGVGAPIWVHPDDRMLWDVV
                     WPDRAPGADLTDGQVIEVGGSNLQVLHTPGHSPGGCCLHASADSVVFSGDTLFNGGPG
                     ATGRSFSDYSTIVASIRDRLLVLDPATVVHTGHGDSTTIGDEAERSGDW"
     gene            646..1446
                     /locus_tag="VFP02_02375"
     CDS             646..1446
                     /locus_tag="VFP02_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9541897.1"
                     /translation="MEVRGVVLAVEEAGTGTPVLWGHGLTSSMAAEVAMGFGVVDLPE
                     DRYRVVRYDARGHGDSGGTTGPAAYAYPGLAADQLGLADALGIDRFVVGGMSMGSGTA
                     LHTAVAAPDRVLALVLGIPPTAWEGRRERGAVYGDRGRVIAEQGMDAFIEQAMAEPLA
                     EIFAPFAEQVTAGIRARYESYDPAVLAPLLTGVGGSDLPDPEAVAMLPMPVLVLAWDG
                     DPVHPRATAERLAELLPQAEVMIAPTLRDVLGWSGRVLEFLDRVVQAK"
     gene            complement(1434..2012)
                     /locus_tag="VFP02_02380"
     CDS             complement(1434..2012)
                     /locus_tag="VFP02_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9541898.1"
                     /translation="MVATPSTMLPLGAELPAFTLSGPDATTWSTEDAVGAPALVVAFL
                     CPHCPYVKHVEARFAEVTADLMNRGAAVVAVQSNAVEDYPQDGPEGMAAQAITHGFRF
                     PYLLDASQEVAKAFRAACTPDFFVFDGEQRLAYRGQFDDSRPSNGLPATGADLMAAVE
                     ALLDGGDVSEDQAPSIGCNIKWRAGNEPDYFA"
     gene            2084..2848
                     /locus_tag="VFP02_02385"
     CDS             2084..2848
                     /locus_tag="VFP02_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9541899.1"
                     /translation="MTPGLPPEAEAHYRSYDEDARLERHSLERVRTQMLLERLLPDPP
                     ADVLDVGGGTGVHSIWLAEQGHRVTLVDAMPEHVDQASAIGTFAATVGDARALDRADA
                     TADAVLLLGPLYHLTEPGDRALALAEARRVLRPGGVLVAGAVSYAASLLDGVREGWMD
                     EPVFVEIVRADLATGYHRNPDADPRWFTTTFFHRPEELRSEVEGAGFVVDEVIGVEGP
                     GWMVGEPEGSLLAAELADAHPELALLSAHLVAVGRR"
     gene            2848..3180
                     /locus_tag="VFP02_02390"
     CDS             2848..3180
                     /locus_tag="VFP02_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET9541900.1"
                     /translation="MADWVAVATEVAPGQIVDAAHGDLDLVVWRTFEGEPCVMEARCP
                     HEWSHLAAEGVVDGPEIVCAAHFWRFTTDGTGSKVGMTGRRDPKADIAVFPCREVDGR
                     IEAQLPSE"
     gene            3192..>3796
                     /locus_tag="VFP02_02395"
     CDS             3192..>3796
                     /locus_tag="VFP02_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9541901.1"
                     /translation="MTELDGARILITGPTGQVGLPVALALAEHNDVVGVARFGDARAR
                     ERLEGAGVTCITADLVGGDFSAVPTDVDHVANFAVAKTGDFEKDLAANVESLGMLMHH
                     CREATSFLHCSSTAVYEPAGHHDFVEGDPLGDNHRPFGFLPTYSITKIAAEAMARYGA
                     RQWDLPTTIARLSVPYGDNGGWPAFHFEMMLAGQAVPVHEDA"
BASE COUNT          503 a         1337 c         1397 g          559 t
ORIGIN      
        1 cggtcgtcgt cctctgatgg cgctgcgcat cgacaacgtc atcacctcgg gcatcttcag
       61 cctggacggc gaggacttcg aggtcgacaa caacatctgg ctggtcggcg acgacgacga
      121 ggtgatcgtg gtcgacgccg cccacgacca ccggccgatc gtggccggcg tgggctcccg
      181 acgggtcgtc gccgtggtct gcacccatgg ccacaacgac cacatcaacg ccgccgtgcc
      241 cctggccgag ggggtcgggg cgccgatctg ggtccatccc gacgatcgga tgctgtggga
      301 cgtcgtctgg cccgaccggg cgccgggtgc cgacctcacc gacgggcagg tcatcgaggt
      361 cggcgggtcg aacctccagg tgttgcacac gcccggtcac tcgcccggcg gttgctgcct
      421 gcacgcctcg gcggacagcg tcgtcttctc cggcgacacc ctcttcaacg gcgggcccgg
      481 cgccaccggt cggagcttct cggactacag caccatcgtg gccagcatcc gggaccggtt
      541 gctggtgctg gacccggcaa cggtggtgca caccggccac ggcgacagca ccacgatcgg
      601 cgatgaggct gagcggtcgg gcgactggtg accgcgggcc aatcggtgga ggtccggggg
      661 gtcgttctcg cggtcgagga agcgggtacc gggacaccgg ttctctgggg gcacgggctg
      721 accagctcga tggcggccga ggtggcgatg ggcttcggtg tcgtcgacct ccccgaggac
      781 cgctaccggg tggtgcgcta cgacgctcgg ggccacgggg actcgggcgg aacgaccggc
      841 ccggccgcct acgcctatcc gggactggcg gccgaccagc tgggcctggc cgacgccctc
      901 gggatcgacc gcttcgtcgt cggcggcatg tcgatgggtt cggggacggc gctgcacacc
      961 gcagtcgcgg ccccggatcg ggtgctcgcg ctcgtcctcg ggatcccgcc gacggcctgg
     1021 gaggggcgcc gcgaacgggg ggcggtctac ggggatcgtg gccgcgtcat cgccgagcag
     1081 gggatggacg ccttcatcga gcaggccatg gccgagccgt tggcggagat cttcgccccc
     1141 ttcgccgagc aggtgaccgc cggcatccgc gcccgttacg agagctacga cccggccgtg
     1201 ctggcccccc tgctcaccgg tgtgggcggc tccgacctcc cggatcccga ggccgtggcc
     1261 atgctcccga tgcccgtcct cgtcctggcc tgggacgggg atcccgtgca cccccgggcg
     1321 accgcggagc gcctcgccga gctcctgcca caggcggagg tcatgatcgc cccgacgctg
     1381 cgcgacgtgt tgggctggtc cggccgggtg ctcgagttcc tggaccgggt ggttcaggcg
     1441 aagtagtccg gctcgttgcc ggcccgccat ttgatgttgc agccgatgct gggggcctgg
     1501 tcttcggaca cgtcgcctcc gtccaggagc gcttcgaccg cggccatcag gtcggcaccg
     1561 gtggcgggca ggccgttgct cggccgggag tcgtcgaact ggcctcggta ggccagccgc
     1621 tgctcgccgt cgaacacgaa gaagtccggg gtgcaggcgg ccctgaacgc cttggccacc
     1681 tcctgggagg cgtcgagcag gtaggggaac cggaagccgt gggtgatggc ctgggcggcc
     1741 atgccttcgg gtccgtcctg ggggtagtcc tcgacggcgt tgctctgcac ggcgaccacg
     1801 gcggcacccc ggttcatgag gtcggcggtg acctcggcga agcgagcctc cacgtgcttg
     1861 acatacgggc agtgcgggca gaggaaggcc accaccaggg ccggggcgcc gacggcgtcc
     1921 tcggtcgacc aggtggtggc atcggggccc gagagggtga aggcgggcag ctcggcgccg
     1981 agcgggagca tcgtggacgg ggtggcgacc atggggtcct ccgggtggcg gtggtggacg
     2041 gaaagccgac ggcagcggtg gaacgagcgc ctaccgtgcc gctgtgactc caggtctacc
     2101 ccccgaggcc gaggcccact accggagcta cgacgaggac gctcgcctgg aacgccactc
     2161 gctcgagcgg gtgcgcaccc agatgctgct ggagcggctg ctgcccgacc ccccggccga
     2221 cgtcctggat gtgggcggcg gtaccggtgt ccactcgatc tggctggcgg agcagggcca
     2281 tcgggtcacc ttggtcgacg ccatgcccga gcacgtcgac caggcgtcgg ccatcggcac
     2341 gttcgcggcc acagtcggcg atgcccgggc gctggaccgg gccgacgcca ccgctgacgc
     2401 cgtgctcctg ttgggtccgc tgtaccacct gaccgagccc ggcgaccggg ccctcgctct
     2461 ggcggaggcg cgccgggtgc tccgccccgg tggtgtgctg gtcgccgggg ccgtgagcta
     2521 tgccgcgtcc ctcctggacg gcgtccggga gggctggatg gacgagccgg tgttcgtcga
     2581 gatcgtccgg gccgacctcg cgaccggcta ccaccgcaac cccgacgccg atccgcgctg
     2641 gttcaccacg accttcttcc accggcccga ggagctgcgg tccgaggtgg agggcgccgg
     2701 cttcgtggtc gacgaggtca tcggggtcga gggaccgggc tggatggtgg gggagccgga
     2761 gggctcgctc ctggcggccg agctggccga cgcccacccc gagctggccc tcctgagcgc
     2821 ccacctcgtc gccgtcgggc gccgctgatg gcggactggg tggcggtggc gacggaggtg
     2881 gccccgggac agatcgtgga cgccgcccac ggcgacctgg atctcgtcgt ctggcggacc
     2941 ttcgagggcg agccctgcgt catggaggcc cggtgccccc acgagtggtc ccacctcgcc
     3001 gccgagggag tggtcgacgg ccccgagatc gtctgcgcgg cccacttctg gcggttcacc
     3061 accgacggca cgggcagcaa ggtcggcatg accggtcgcc gggaccccaa ggccgacatc
     3121 gccgtgttcc cgtgccggga ggtcgacggt cgaatcgagg ctcagctccc cagcgagtag
     3181 aacgaacggc gatgaccgag ctggacggtg cccgcatcct catcaccggt ccgacgggtc
     3241 aggtcgggct ccccgtggcc ctcgccctgg ccgagcacaa cgacgtcgtc ggggtcgccc
     3301 gtttcggcga cgcccgggct cgggagcggc tcgaaggggc cggcgtgacc tgcatcaccg
     3361 ccgatctcgt cggcggggat ttctcggccg tgcccacgga cgtcgaccac gtcgccaact
     3421 tcgccgtggc caagaccggc gacttcgaga aggacctggc cgccaacgtc gagtccctcg
     3481 ggatgttgat gcaccactgc cgggaggcca cctctttcct gcactgctcg tccaccgcgg
     3541 tgtacgagcc cgccggccac cacgacttcg tcgagggcga tccgctgggg gacaaccacc
     3601 gcccgttcgg cttcctgccc acctactcga tcaccaagat cgcggcggag gcgatggccc
     3661 gctacggcgc ccgccagtgg gacctgccca ccaccatcgc ccgcctgtcg gtgccctacg
     3721 gcgacaacgg cggctggccc gccttccact tcgagatgat gctggccggc caggccgtgc
     3781 ccgtccacga ggacgc
//
LOCUS       DASTFV010000129         3417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_459260, whole genome shotgun sequence.
ACCESSION   DASTFV010000129 DASTFV010000000
VERSION     DASTFV010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459260"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VFP02_02400"
     CDS             complement(<1..611)
                     /locus_tag="VFP02_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET9541902.1"
                     /translation="MTDFLNDWGLTLVTFIPAVGAVLVMAMPKGDDLLVKVVALAASL
                     GSGVMGVLLLLDFDYDHAGELQYVVDKSWIDVINSRYIMGLDGISLPLLALTLLVVPL
                     CIIYSWNHLPDPGKPKAFFTLLLILETGMVGTFLAQDLILFFVFFEVVLLPMYFMIGV
                     WGGPNRQYAAIKFFLFTLFGSALMLVSFLALYFLGDGTFDIRIL"
     gene            complement(628..2709)
                     /gene="nuoL"
                     /locus_tag="VFP02_02405"
     CDS             complement(628..2709)
                     /gene="nuoL"
                     /locus_tag="VFP02_02405"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET9541903.1"
                     /translation="MAMWFLEHAWIIPFLPAVSFLLILAVGKRLPFGGSEIGILSVGT
                     SFVLSACTGVAWIQHVNAGGGGEGGEEALGLAARAAEEGHHAIEPIVKTVTWFQFGGH
                     EFPVGTMIDGLSVIMLVTVSLVSLLVHVYSTEYLKGDRRFTHYFAFLSLFTASMLTLV
                     IAQNTLMLLTAWELVGLCSFVLIGHWWEDKPNSDAALKAFLTNRVGDVGLIIGVIILF
                     FGAGTFNIVDINALAAEGGLSHLILLMAAICLTAAVCSKSGQFPLHTWLPDAMAGPTP
                     VSALIHAATMVVAGVFMVARFYPVFFEGFSISSSSINLLAFVGAFTTVVGATLAFVQK
                     DIKKVLAYSTISQLGYMVMALGVGAWTAAIFHLFTHAMFKACLFLGAGSVSHAAHHTF
                     DMRKMGGLRKYMPSTFATFVIASLALAGIPPLAGFWSKDEILAGALQGQTNAYPAMLV
                     LGCMTAFMTAAYMTRAIWMTFFGEYRGEGTPHESPRLMTVPLWILAGAAVVVGFFNLP
                     EKLAPDSIALRFEHYIEPTFAFPEIEHPEFSFLLAAFSILLAVSGLALAWRYFAHNRG
                     PHGLTERSAAAAWGYRVLENKYYLDWLYESVIVRSIKGPIANGVYWFNQHILDGVVNG
                     AANVSRVTGNWLYRSIDQGGVDRLVNASGAGAEGSGQLLRRIQTGRVQQYAALLFAGA
                     AVLAGIFIVII"
     gene            complement(2709..3008)
                     /gene="nuoK"
                     /locus_tag="VFP02_02410"
     CDS             complement(2709..3008)
                     /gene="nuoK"
                     /locus_tag="VFP02_02410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET9541904.1"
                     /translation="MLLNQFLLLGAVLFCIGVYGVLSRRNGVAVLMSVELILNAVSIN
                     LVAFGAFNGDVAGQVFALFVITVAAAEVGVGLAIVLLIYRNRRSVDLDQADLMKG"
     gene            complement(3014..>3417)
                     /locus_tag="VFP02_02415"
     CDS             complement(3014..>3417)
                     /locus_tag="VFP02_02415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET9541905.1"
                     /translation="LASVAAQYILLAAEFVAATQVLVYIGAIVVLFLFGIMLTRAKLG
                     RDQDLTNKRWPMAAATALLLFGVMTYALIDQYHWNETPMPADPRITTVEGSNTATVSD
                     SIFSTYLVPFEVVSVLLLAALVGAIVIARKD"
BASE COUNT          662 a         1031 c         1246 g          478 t
ORIGIN      
        1 aggatgcgga tgtcgaaggt gccgtcgccc aggaagtaga gggccaggaa gctgaccagc
       61 atcaaggccg agccgaacaa cgtgaacagg aagaacttga tcgccgcgta ctggcggttg
      121 ggcccgcccc agaccccgat catgaagtac atcgggagca ggacgacctc gaagaacacg
      181 aagaagagaa tcaggtcctg ggccaggaag gtcccgacca tgcccgtctc caggatgagc
      241 aggagggtga agaaggcctt cggcttgccc gggtcgggca ggtggttcca cgagtagatg
      301 atgcagagcg gcaccaccag cagggtcagg gccagcagcg gcaacgagat cccgtcgagg
      361 cccatgatgt agcggctgtt gatgacgtcg atccagctct tgtcgacgac gtactgcagc
      421 tcgccggcgt ggtcgtagtc gaagtccagg agcagcagga cacccatcac cccggacccg
      481 agcgaggccg ccaaggcgac caccttcacc agcaggtcgt cccccttggg catggccatc
      541 accaggacgg cgcccaccgc cgggatgaac gtcaccaggg tcaggcccca gtcgttgagg
      601 aagtcggtca tggctcggtg gtgactccta gatgatgacg atgaagatgc cggcgaggac
      661 tgcggcgccg gcgaagagga gggcggcgta ctgctgcacg cggccggtct ggatgcgacg
      721 caggagctgg ccggagccct cggccccggc gcccgaggcg ttgaccagcc ggtccacgcc
      781 tccctggtcg atgctgcggt agagccagtt gcccgtgacc ctggacacgt tggccgcccc
      841 gttgaccacg ccgtcgagaa tgtgctggtt gaaccagtag acgccgttgg cgatgggacc
      901 cttgatgctc cggacgatga cgctctcgta gagccagtcg aggtagtact tgttctcgag
      961 cacgcggtaa ccccaggcgg ccgccgcact gcgttcggtg aggccgtgcg ggccgcggtt
     1021 gtgggcgaag taccgccaag ccagcgcgag gccggagacg gccaacagga tcgagaaggc
     1081 cgccagcagg aagctgaact cggggtgctc gatctccggg aaggcgaagg tcggttcgat
     1141 gtagtgctcg aagcgcagcg cgatgctgtc cggggccagc ttctcgggca ggttgaagaa
     1201 gccgacgacg acggccgccc cggccaggat ccagaggggc acggtcatga gccggggcga
     1261 ctcgtggggg gtgccctcac cccggtactc gccgaagaag gtcatccaga tggcccgggt
     1321 catgtaggcc gcggtcatga acgccgtcat gcagccgagg accagcatcg ccgggtaggc
     1381 gttggtctgg ccctgcagcg ccccggccag gatctcgtcc ttggaccaga accccgccag
     1441 cggcgggatg ccggccaggg ccagggaggc gatgacgaag gtggcgaagg tgctgggcat
     1501 gtacttgcgc agcccgccca tcttgcgcat gtcgaaggtg tggtgggcgg cgtggctgac
     1561 cgaccccgcc ccgaggaaga ggcaggcctt gaacatggcg tgggtgaaga ggtggaagat
     1621 ggccgccgtc caggccccca cgccgagagc catgaccatg tagccgagct ggctgatggt
     1681 ggagtaggcg aggaccttct tgatgtcctt ctggacgaag gccagcgtcg ccccgacgac
     1741 ggtggtgaag gccccgacga aggccagcag gttgatgctg gaggagctga tcgagaagcc
     1801 ctcgaagaac acggggtaga agcgggcgac catgaagacg ccggccacga ccatcgtggc
     1861 cgcgtggatc agggccgaga ccggggtggg gccggccatg gcgtcgggca accaggtgtg
     1921 cagggggaac tggcccgact tcgagcagac cgcggccgtc aggcagatgg cggccatgag
     1981 gaggatgagg tggctcaggc cgccctcggc cgccagcgcg ttgatgtcga cgatgttgaa
     2041 ggtgccggcg ccgaagaaca ggatgatcac gccgatgatc aggccgacgt cgccgacccg
     2101 gttggtcaga aaggctttga gggcggcgtc ggagttgggc ttgtcctccc accagtgccc
     2161 gatgagcacg aacgagcaga gcccgaccag ctcccacgcg gtcagcagca tcagggtgtt
     2221 ctgggcgatg accagggtga gcatcgacgc cgtgaacagg gacaggaagg cgaagtagtg
     2281 ggtgaagcgc cggtccccct tcaggtattc ggtgctgtac acgtggacca gcagcgacac
     2341 caggctgacg gtgacgagca tgatcaccga caggccgtcg atcatggtcc cgacggggaa
     2401 ctcgtggccg ccgaactgga accaggtgac ggtcttcacg atcggctcga tggcgtgatg
     2461 gccctcttcg gcagcccggg ccgcgaggcc cagggcctcc tcgcccccct cgccgccccc
     2521 gccggcgttg acgtgctgga tccaggccac cccggtgcag gcggagagca cgaaggaggt
     2581 gccgaccgac aggatcccga tctccgagcc gccgaacggg agccgcttgc ccacggccag
     2641 gatgagcagg aacgagacgg ccgggaggaa cgggatgatc caggcgtgtt ccaggaacca
     2701 catggccatc agcccttcat gaggtcggcc tggtccaggt cgacgctccg gcggttgcgg
     2761 tagatgagca ggacgatggc caggccgaca ccgacctcgg cggcggcgac ggtgatgacg
     2821 aacagggcga acacctggcc ggcgacgtcg ccgttgaacg caccgaaggc gaccaggttg
     2881 atgctgacgg cattgaggat gagctcgacc gacatcagca cggccacgcc gttgcggcgg
     2941 gagagcacgc cgtagacgcc gatgcagaac aggaccgcgc cgaggaggag gaactggttg
     3001 agcagcatcc tccctagtcc ttcctggcga tcacgatggc ccccacgagg gcggcgagga
     3061 ggagcacgga cacgacctcg aagggcacga ggtaggtgga gaagatcgag tcgctcaccg
     3121 tggcggtgtt ggagccctcc accgtggtga tccgcgggtc cgccggcatc ggcgtctcgt
     3181 tccagtggta ctggtcgatg agcgcgtagg tcatcacccc gaacaagagc agcgcggtgg
     3241 ccgccgccat gggccagcgc ttgttggtga ggtcttggtc gcggccgagc ttggcgcggg
     3301 tgagcatgat gccgaacagg aacagcacga cgatggcgcc gatgtacacg aggacctggg
     3361 tggccgcgac gaactcggcg gccagcagga tgtactgcgc cgccaccgag gcgagca
//
LOCUS       DASTFV010000130         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_461079, whole genome shotgun sequence.
ACCESSION   DASTFV010000130 DASTFV010000000
VERSION     DASTFV010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461079"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VFP02_02420"
     CDS             complement(<1..414)
                     /locus_tag="VFP02_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET9541906.1"
                     /translation="MPSTSPAGIRTRLLAAPVLVGAAVLVLAGPVGAAVVAETTEAVG
                     SQMTWGKAAVLGVVEGITEFLPISSTGHLLVASRLLDLPSEEGSAGLEALNAYAIAIQ
                     FGAILAVVALFWRRFVEMLQGLAGRNPDGRHLFVIL"
     gene            complement(465..971)
                     /locus_tag="VFP02_02425"
     CDS             complement(465..971)
                     /locus_tag="VFP02_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET9541907.1"
                     /translation="MTGDPGAQVRESLDATGVAYEVMDCDPELADTAVFCEHYGVPVE
                     QSANTILVAGKPRGGGDKVYVACVVLAHTRLDVNNAVRHRLDVKKASFASADETRELT
                     GMLIGGVTAPALPDGMPLWVDARVMDAAWIVLGGGDRSTKIKAPPAVLTALGAEVVED
                     LATIPPAG"
     gene            complement(940..1551)
                     /locus_tag="VFP02_02430"
     CDS             complement(940..1551)
                     /locus_tag="VFP02_02430"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HET9541908.1"
                     /translation="MLWWLVVVGLSLTVGLVLESGLSRARADARRFGEPVPVVVARVD
                     LAAGAMAGPDTVELRSWPAALVPAGALTAVPDGEVVSQAVLAGEPVVEARLGADHLVP
                     PGSRALALPTGPGALGVRPGDRVDLLATFDPLVAPAGEDPTVTVARAATVVAVRTRAI
                     TVAVTAAEAPRVAFALSQATITLALTPPGTVPADDGGSGSTGP"
     gene            complement(1768..>2108)
                     /locus_tag="VFP02_02435"
     CDS             complement(1768..>2108)
                     /locus_tag="VFP02_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541909.1"
                     /translation="VVVIEGPRFSTRAESAWFAAAGWSVVNMTQYPEAALARELGLCY
                     ATVALITDYDVGVEGVADHAVTMDEVFAVMADNNDRVRTLLFRAIDAIPSTRSCACIE
                     GAGGLQPDPP"
BASE COUNT          311 a          728 c          835 g          234 t
ORIGIN      
        1 caggatgacg aacaggtggc gcccgtcggg gttgcgcccg gcgaggccct ggagcatctc
       61 gacgaagcgg cgccagaaca gggcgaccac ggccaggatg gcgccgaact ggatggcgat
      121 ggcgtaggcg ttgagggcct cgaggccggc gctgccctcc tccgacggga ggtcgagcag
      181 ccgcgacgcg acgagcaggt gcccggtcga cgagatgggc aggaactcgg tgatgccctc
      241 gaccaccccg aggacggccg ccttccccca cgtcatctgg ctgccgacgg cctccgtcgt
      301 ctccgccacc acggccgccc cgaccgggcc ggcgaggacc agtacggcgg caccgaccag
      361 cacaggagcc gccagcaggc gcgttcggat gccggcgggc gacgtcgagg gcacgcccga
      421 gtttccgcca gagcggcgcc tgggcgccat tcggagaagc cgactcaacc ggcgggcggg
      481 atggtggcga ggtcttccac gacctcggcg ccgagggcgg tgagcacggc gggtggggcc
      541 ttgatcttgg tgctgcgatc cccgccgccg aggacgatcc acgccgcgtc catgacccgg
      601 gcatcgaccc acagcggcat gccgtcgggc agggccggcg ccgtgacgcc gccgatgagc
      661 atcccggtca gctccctcgt ctcgtcggcc gaggcgaacg acgccttctt cacatcgagg
      721 cggtggcgca cggcgttgtt cacgtcgagg cgggtgtggg ccaggacgac gcaggccacg
      781 tagaccttgt cgcccccgcc ccggggcttg cccgccacca ggatggtgtt ggccgactgc
      841 tcgacgggga cgccgtagtg ctcgcagaag acggcggtgt cggccagctc ggggtcgcag
      901 tccatgacct cgtaggccac acccgtcgcg tcgagcgact cacggacctg tgctcccgga
      961 tcccccgtca tcggcgggga cggtacccgg aggggtgagg gcgagcgtga tggtggcctg
     1021 cgagagggcg aaggcgaccc gaggggcctc agccgcggtg acggccaccg tgatcgcgcg
     1081 ggtccggacg gcgacgacgg tggcggcccg ggcgacggtc accgtggggt cctcgcccgc
     1141 gggggcgacc agcgggtcga aggtggccag caggtcgacc cggtcaccgg gtcggacacc
     1201 gagggcgccg ggcccggtgg ggagggccag ggcccggctg ccgggcggca ccaggtggtc
     1261 ggcgccgagt cgggcctcga ccacgggctc gccggcgagg accgcctgac tcaccacctc
     1321 accgtcgggc acggcggtca gggccccggc ggggacgagg gcggcgggcc acgagcgcag
     1381 ctcgaccgtg tcggggccgg ccatggcccc cgcggccagg tcgacgcgag ccacgacgac
     1441 agggacgggc tcgccgaagc ggcgggcgtc ggcccgggcg cggctgaggc cggactcgag
     1501 gacgaggccg acggtcagcg acagcccgac gacgaccagc caccagagca ccgggcgccg
     1561 tcgcagccgg agccgggccc accagagcgc gagcggacgg aggggagagg gacgaggtcg
     1621 ccgcacggcg cgcctccttc gcacgagtga ctccgcggga gaagcgccgc ggtcagcgta
     1681 cgcggacccg gccgattccc gttctggttg cgtccagccg cgctggggcg cgggagccgc
     1741 caccagaacg atcggggggt cagggcgtca gggcgggtcg ggctggaggc caccggcgcc
     1801 ttcgatgcag gcgcacgacc gggtcgacgg gatggcgtcg atggcccgga agaggagggt
     1861 gcggacccga tcgttgttgt cggccatgac ggcgaacacc tcgtccatgg tcacggcgtg
     1921 gtcggcgacc ccctcgacgc cgacgtcgta gtcggtgatc agggccacgg tggcgtagca
     1981 gaggcccagc tcccgggcca gggcggcttc ggggtactgg gtcatgttga cgaccgacca
     2041 acccgcggcg gcgaaccagg ccgactcggc ccgggtggag aaacgcggcc cttcgatcac
     2101 gaccacgg
//
LOCUS       DASTFV010000131         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_462035, whole genome shotgun sequence.
ACCESSION   DASTFV010000131 DASTFV010000000
VERSION     DASTFV010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462035"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VFP02_02440"
     CDS             complement(<1..425)
                     /locus_tag="VFP02_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET9541910.1"
                     /translation="MAEVAVVGAGYVGLTTAACFAHLGHKVVCADIDVARIEALVRGE
                     VPIVEQGLDAVVREGLDSGRLSFVVGAPAAAAQAEFVYLAVPTPQGADGRADLSYIER
                     AAHEIGPVLAPEAIVINKSTVPVGSTGVVERALERSDVYV"
     gene            complement(430..1899)
                     /locus_tag="VFP02_02445"
     CDS             complement(430..1899)
                     /locus_tag="VFP02_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET9541911.1"
                     /translation="MAGRRARERRTWPQRLLITFNAGFIVACLVSAAALGYANEKLDQ
                     VPRIDFGENGPLAEPEAPGEAQNYLLVGTDSSAGLDPDDSRTNGRENLGVLSDTIMLL
                     RVDPGSTQAQLLSFPRDLWVTIPGAGEQKINAALAAGGAETLINTITDNFGVPVHHYI
                     EVDWAGFQNLVEAVDGVPMYFDKPLRDETTGLLIPHPGCVTLDPEQALAFARSRHLEY
                     SEDGGATYAVDGTGDLGRISRQQAFLRRAIQRAIDKGVRNPITLNSLVDVGIGAVGAL
                     DTELSADDLITLGTAFRNFQPDELQTMTLDVYDDVVNGLSVLRLQDTEANQERLDIFK
                     GLGSGAVGESGSVRVAINNGTGATGQATAVADSLAGLGFDTSPGTGDAERFDFTRTVV
                     RYQPGNEAAAQYVAAQLVNGADLQEVGATYVADVILVTGTDYAGVVESLQPPPTPLGG
                     AGGGTVPGTTPADPGDGSVTTTTYAYGEVPSTPADQVCG"
     gene            1971..>2985
                     /locus_tag="VFP02_02450"
     CDS             1971..>2985
                     /locus_tag="VFP02_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HET9541912.1"
                     /translation="MRRGPVDGALAVVGDRLATDLIDLTQDLTALDGDGFWAVVIPFD
                     GAPVCARFATVRPAVPWPGDRWRGPRPDQWTSSLDRATFEAGVRDIRAAIGRGDVYQV
                     NLTRRLSAPHDLGAGTAGDIAALGAALAAGNPAPYAAVVRVPGADLAVASASPEQFLR
                     REGDRVWSSPIKGTAATADRLTAKDRAENVMIVDLVRNDLGRVCAYGSVHVPSLCRVE
                     PHPGLVHLVSTVEGRLRPDLGWPELIAAAFPPGSVTGAPKLAAVEHLRRLEPVARGVY
                     CGAVGWVDADAGVGELNVAIRTFWMDEGRLHFGTGGGITWDSDPHAEWQETELKARRL
                     LAVA"
BASE COUNT          400 a         1037 c         1102 g          446 t
ORIGIN      
        1 acgtacacgt cggagcgctc cagggcccgc tcgaccaccc cggtcgaacc gacgggcacc
       61 gtcgacttgt tgatgacgat cgcctcgggg gccaacacgg ggccgatctc gtgggcggct
      121 cgttcgatgt agctgaggtc ggctctgccg tcagccccct ggggtgtggg cacggccagg
      181 tagacgaact cggcctgggc ggcggcggcg ggggcgccga ccacgaagct caggcggcca
      241 ctgtccaggc cctcccggac gacggcgtcg aggccctgct cgacgatggg gacctcacct
      301 cggaccaggg cctcgatccg ggcgacgtcg atgtcggcac agacgacctt gtggcccaga
      361 tgggcgaagc acgcggcggt cgtcagcccg acgtaaccag cccctacgac agcgacctcg
      421 gccacggcgc tacccgcaga cctggtcggc cggggtgctg ggcacctcgc cgtaggcata
      481 ggtcgtcgtg gtcaccgagc cgtccccggg gtcggccggc gtcgtcccgg gcacggttcc
      541 gccgcccgcg ccccccagag gggtcggggg cggctgcaag ctctcgacga ccccggcgta
      601 gtccgtgccg gtgaccaaaa tgacgtcggc gacataagtg gctcccacct cttgaagatc
      661 ggcaccgttc acgagttgtg cagcgacgta ctgggccgcg gcctcgtttc cgggctggta
      721 acgcaccacc gtacgcgtga agtcgaacct ctcggcatct ccggtgcccg gcgatgtgtc
      781 gaagccgagg ccggccaggc tgtcggccac cgccgtggcc tggcccgtcg cgcccgtccc
      841 gttgttgatg gccacgcgga ccgagccgct ctcgccgacg gctcccgacc ccaggccctt
      901 gaagatgtca aggcgctcct gattggcctc ggtgtcctgg agccgcagga cggagaggcc
      961 gttgacgacg tcgtcgtaga cgtcgagggt catggtctgc agctcatcgg gctggaagtt
     1021 gcggaaggcc gtccccaggg tgatcaggtc gtcggcgctg agctcggtgt ccagcgcccc
     1081 gacggcgccg atgcccacgt cgacgagcga gttgagggtg atcgggttgc gcacgccctt
     1141 gtcgatcgcc cgttggatcg cccggcgcag gaaggcctgc tggcgggaga tgcgtccgag
     1201 gtcgccggtg ccgtcgaccg cgtaggtggc gccaccgtcc tcggagtact cgagatgccg
     1261 ggaacgggcg aaggcgaggg cctgttcggg atcgagggtg acacagcccg gatgggggat
     1321 cagcaacccg gtcgtctcgt cgcgcaaggg cttgtcgaag tacatgggca cgccgtcgac
     1381 ggcctcgacc aggttctgga acccggccca gtcgacctcg atgtagtggt ggacgggcac
     1441 cccgaagttg tcggtgatcg tgttgatcag cgtctcggcc ccaccggcgg ccagggcggc
     1501 gttgatcttc tgctcgcccg caccggggat ggtcacccag aggtcccggg ggaacgacag
     1561 gagctgggct tgcgtcgagc cggggtcgac ccgcagcagc atgatcgtgt cggacagcac
     1621 gccgaggttc tcccggccgt tggtgcggga atcgtcgggg tccaggccgg ccgagctgtc
     1681 ggtgcccacc agcaggtagt tctgggcctc gccgggcgct tcgggctcgg ccagcggccc
     1741 gttctcgccg aagtcgatgc gcggcacctg gtcgagcttc tcattcgcat accccagggc
     1801 cgccgccgag accaggcacg cgacgatgaa gccggcgttg aaggtgatca gcaaccgctg
     1861 gggccaggtg cggcgctcac gcgcccgtcg accggccatc ggggcgaggc tagcggccat
     1921 ttccagaagt ccggaggcgg ccctaccggt cgccccgtag cctccgcagg gtgcgacggg
     1981 gaccggtgga cggggcgctg gcggtggtcg gcgaccgcct ggccaccgac ctgatcgacc
     2041 tcacccagga cctcacggcc ctcgacggcg acggcttctg ggcggtggtg atccccttcg
     2101 acggcgcgcc ggtgtgcgcc cggttcgcca cggttcgccc ggccgtcccc tggcccgggg
     2161 accggtggcg ggggcctcgg cccgaccagt ggacctcgtc gctggaccgg gcgacgttcg
     2221 aggccggggt gcgggacatc cgggccgcca tcgggcgggg cgacgtctac caggtgaacc
     2281 tgacccgccg gttgtccgct ccccatgacc tgggcgcggg gacggccggg gacatcgccg
     2341 ccctcggcgc cgcgctggcc gccgggaacc ccgcgccgta cgccgcggtc gtgcgggtgc
     2401 cgggggctga cctggccgtg gcatcggcct ctcctgagca gttcctccgt cgggaggggg
     2461 accgcgtgtg gtcctccccc atcaagggga cggcggcgac ggccgatcgc ctcacggcca
     2521 aggaccgggc cgagaacgtg atgatcgtgg acctggtccg caacgacctc ggtcgggtgt
     2581 gcgcctacgg ctcggtccac gtgccatccc tgtgccgggt cgagcctcac cccggcctgg
     2641 tccacctggt cagcaccgtg gagggccggc tgcgaccgga cctgggctgg ccggagctga
     2701 tcgccgccgc cttcccgccc ggatcggtga ccggggcgcc gaagctggcc gccgtcgagc
     2761 acctgcgccg cctggaaccg gtcgctcggg gcgtctactg cggggcggtg ggctgggtgg
     2821 atgccgatgc cggcgtgggc gagctcaacg tcgccattcg caccttctgg atggacgagg
     2881 ggcgtctgca cttcggcacc ggaggcggca tcacctggga ctcggaccct cacgccgaat
     2941 ggcaggagac cgagctgaag gcccgccgcc tcctcgccgt cgcct
//
LOCUS       DASTFV010000132         2347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_466117, whole genome shotgun sequence.
ACCESSION   DASTFV010000132 DASTFV010000000
VERSION     DASTFV010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466117"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFP02_02455"
     CDS             <1..346
                     /locus_tag="VFP02_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9541913.1"
                     /translation="NVPSDLRDATALDGYGHLGFMWHVAIPLARPAIAGLSVFSFLYA
                     WNQYLWPLLVADDPDVRTVQIGLKSLNSANVDELNLVMAGTLIAALPIALVLIVFQRH
                     LVRGLTAGAVKG"
     gene            351..1787
                     /locus_tag="VFP02_02460"
     CDS             351..1787
                     /locus_tag="VFP02_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9541914.1"
                     /translation="MKRTSLVLTLLAVLALVAAACGGDSGGGGGGGEGAQGRDAEVDA
                     SLCPVDTLEAASADGPVQIEFWHAMTASNEETLNTMIDDYNASQDAVEVTGVFSGSYD
                     ETLDKYLTGLRSGELPQLVQLEETTLQTMIDSGSTVPAAACVAASDYDTSDILPSVLA
                     EFEVEGTLWPMPFNVSNPVFYYDRNDFTAAGLDPDAPPATLDEMYDMGQTIVRAGAAE
                     HAMSLEVQPWYPEQWMSMAGETLVDNTNGRDARAEAATVDNATMIDIFTWIREMVDND
                     LIFNVGRNPSGADHFFALASGDAAFTIGTSGALGSVYDALDSGTVETDVDIGIGPLPG
                     PVAGGPTSAGGAALSIVANGSSPEEIAATWDFVQYLVDPVQQAFWHVNTGYIPISFTA
                     SEDADVQALWADRPGYQVAYEQLADDNLPPGGGGPVIGAYVEFRNALEDAIEALVLDN
                     ADPTATAASLQEEADTAIASYNDRVADS"
     gene            complement(1800..2081)
                     /locus_tag="VFP02_02465"
     CDS             complement(1800..2081)
                     /locus_tag="VFP02_02465"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HET9541915.1"
                     /translation="MLHHVVLFQVPDPLHREELVTRLQALPGLIPEIDSYEVGVDVSG
                     GDNSYDVGLYSTFADTDALEVYRAHHAHQAVLQLIGEICPYRIVTDWLD"
BASE COUNT          377 a          848 c          781 g          341 t
ORIGIN      
        1 caacgtcccc agcgacctgc gggacgccac ggccctggac ggctacggcc acctcggctt
       61 catgtggcac gtggccatcc ctctggcccg cccggccatc gccggcctga gcgtcttcag
      121 cttcctctac gcgtggaacc agtacctctg gcccctgctc gtagccgacg accccgacgt
      181 ccggaccgtg cagatcgggc tgaagagcct caactcggcc aacgtcgacg aactgaacct
      241 ggtgatggca ggcaccctca tcgccgccct acccatcgcg ctggtcctca tcgtcttcca
      301 gcgccacctg gtgcgggggc tcacggccgg ggcagtgaaa ggatgaccga atgaagcgaa
      361 cctccctggt cctgaccctt ctggccgtgc tggccctcgt ggccgcggcc tgtgggggcg
      421 acagcggggg cgggggtggc ggtggcgagg gcgcccaggg ccgtgacgcc gaggtcgacg
      481 cctcgctctg cccggtcgac accctcgaag cggcgtcggc cgacgggccg gtgcagatcg
      541 agttctggca cgcgatgacc gcctccaacg aggagacgct caacacgatg atcgacgact
      601 acaacgccag ccaggacgcc gtcgaggtca ccggggtctt ctccggttcc tacgacgaga
      661 ccctcgacaa gtacctgacc ggcctgcgca gcggcgagct cccccagctg gtccagctcg
      721 aggagaccac cctgcagacc atgatcgaca gcgggagcac ggtgccggcc gcggcctgtg
      781 tcgcggcgtc ggactacgac acctccgaca tcctcccgtc ggtcctggcc gagttcgagg
      841 tcgagggcac gctctggccg atgccgttca acgtctcgaa cccggtcttc tactacgacc
      901 gcaacgactt cacggcggcc ggcctcgacc ccgacgcccc gccggccacc ctcgatgaga
      961 tgtacgacat gggccagacc atcgtcaggg ccggggcggc cgagcacgcc atgtccctcg
     1021 aggtccagcc ctggtacccc gagcagtgga tgtcgatggc gggggagacc ctggtcgaca
     1081 acaccaacgg ccgggacgcc cgggcggagg cggccaccgt ggacaacgcc acgatgatcg
     1141 acatcttcac ctggatccgg gagatggtgg acaacgacct gatcttcaac gtcgggcgca
     1201 acccgtccgg cgccgaccac ttcttcgccc tcgccagtgg tgacgccgcc ttcaccatcg
     1261 gcacatccgg cgccttgggc tcggtctacg acgccctcga ctccggcacc gtcgagaccg
     1321 acgtcgacat cggtatcggc cccctgcccg gcccggtggc gggaggcccg accagcgccg
     1381 gcggggcggc cctgtcgatc gtggccaacg gctcgtcgcc cgaagagatc gcggccacgt
     1441 gggacttcgt ccagtacctg gtcgacccgg tccagcaggc cttctggcac gtcaacaccg
     1501 gctacatccc gatcagcttc accgcctccg aggatgccga cgtgcaggcc ctgtgggccg
     1561 atcgccccgg ctaccaggtg gcctacgagc agttggccga cgacaacctg ccgccgggcg
     1621 gcggcggccc ggtcatcggg gcctacgtcg agttccgcaa cgccctggag gacgccatcg
     1681 aggccttggt cctcgacaac gccgacccga cggccaccgc ggccagcctg caggaggagg
     1741 ccgacaccgc catcgcctcc tataacgacc gggtcgccga cagctgaccc accagaccgt
     1801 cagtcgagcc agtcggtgac gatgcggtag gggcagatct cgccgatgag ctggagcacg
     1861 gcctggtggg cgtggtgagc ccggtagacc tccagcgcgt cggtgtcggc gaaggtggag
     1921 tagaggccga cgtcgtagct gttgtcgccg ccggatacgt cgacgccgac ctcgtacgag
     1981 tcgatctcgg gtatcagccc gggtagggcc tggagccggg tcaccagctc ctcccgatgg
     2041 aggggatcgg ggacctggaa caacacgaca tggtggagca cgccgccatt ctggccgccg
     2101 cgctctgcgg tccgggcgtg gatcccgcga tccgcggaac cccttcttcc gctgtagccg
     2161 gatggtgtgg gtcagacgat gcggtaggtc tggagggcgt gggccacgac cttgccgtcg
     2221 tcgtcggcgg cagtgacctc gcagaagatc agctcccggc cccggcggat cgcccggccg
     2281 tgacacacca ggtccgagcg gcgggcagcc ccgacgtact ggaccgacag ggcgaccgtc
     2341 gacgcac
//
LOCUS       DASTFV010000133         2507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_477445, whole genome shotgun sequence.
ACCESSION   DASTFV010000133 DASTFV010000000
VERSION     DASTFV010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477445"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1114)
                     /locus_tag="VFP02_02470"
     CDS             complement(23..1114)
                     /locus_tag="VFP02_02470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9541916.1"
                     /translation="MDFAFTEEQEAVRELAEQVFTGTATLERVKEVEASDERMDRALW
                     KELATTGLLGIGLPEAHGGAGLGLVEVCLTLLAQGRRVAPVPLWPTVVAAQTLAAFGT
                     DGHQARWLPAVISGDAVLTVALEVEPGGRGEADEVDGDWRLTGSWPSVPAGHLADAVV
                     VPAAVDGSVALFLVEASAAGTSVEEATTTNRSKVGHYRFDATPAERLEGGADAVTFAH
                     ERALVALAAVSLGACEEATRLAAEYTSGREQFGKPLSTNQGVVLKAADAYIAMAAMRA
                     TLWQAAWRIDEGLDAADAVEVAHWWASDGGHRVVHITQHLHGGMGADIDYPVHRYFLW
                     AKEVTDTLGGPSAHLARLGDLIAQEADDA"
     gene            complement(1116..2045)
                     /locus_tag="VFP02_02475"
     CDS             complement(1116..2045)
                     /locus_tag="VFP02_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional MaoC family dehydratase
                     N-terminal/OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HET9541917.1"
                     /translation="MTADELLDRLRAFEGRSYGPAVTADDPVNLPMIRHWVEAMGDTN
                     PVYLDDGAAEATGRPGIVAPAVMLQVWSMRGLKSQGPRADGVQSELLGLLEEHGFTSV
                     VATNTEQEYLRELVPGDLLATTSVIESVSEEKATGLGVGHFVTTRTEYRDQDGELVGA
                     MTFRILKFRPRPKAEPKPNRPRPAVNLDNAFFFEGAKDEKLLIQKCSSCGALRHPPGP
                     LCPQCQSLDWEAIEASGQGTVYSFVTNHYPQVPAFDYPLNVALIELEEGVRLISNVVG
                     IDADAVEIGMPVAVVFTAFDDELTLPQFQPVGR"
     gene            complement(2045..>2507)
                     /locus_tag="VFP02_02480"
     CDS             complement(2045..>2507)
                     /locus_tag="VFP02_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9541918.1"
                     /translation="ITTQLNHERIGLAAHSGEAHELFADVRDWAAENGVLAIPWVRAD
                     LAKCHALLDAMHLLNWKLTTAVANDELNPADASAVKVYGTEAVVDVYHLLLGVLGAAG
                     YLREGSPGAVLSGRVEQAGRSAQINTFGGGVNEIQREIVAWMGLGMTRGAR"
BASE COUNT          344 a          897 c          905 g          361 t
ORIGIN      
        1 gaaggcccgc gtgggggcga ggtcaggcat cgtcggcctc ctgggcgatc aggtcaccga
       61 ggcgggccag gtgggcgctc gggcccccga gcgtgtcggt gacctccttg gcccagagga
      121 agtaccggtg cacggggtag tcgatgtcgg cccccatgcc gccgtggagg tgctgggtga
      181 tgtgcacgac gcggtgacct ccgtcggagg cccaccagtg ggcgacctcg acggcatcgg
      241 ccgcgtccag gccctcatcg atgcgccaag ccgcctgcca gagcgtggcc cgcatcgccg
      301 ccatggcgat gtaggcgtca gcggccttga gcaccacgcc ctggttggtc gacagcggct
      361 tgccgaactg ctcccggccg gaggtgtact cggcggccag gcgcgtggcc tcctcgcacg
      421 cgccgagcga gacggcggcg agggcgacca gcgcgcgttc gtgggcgaag gtcacggcgt
      481 cggcgccgcc ttcgaggcgc tcggccggcg tggcgtcgaa ccggtagtga ccgaccttgc
      541 tgcgattggt cgtcgtcgcc tcctcgaccg aggtgcccgc ggccgacgct tccaccagga
      601 acagggccac ggacccgtcg actgcggcgg gcaccacgac ggcgtcagcc aggtggccgg
      661 cgggcacgct gggccaggac ccggtcaggc gccagtcccc gtcgacctcg tctgcctcac
      721 cgcgacctcc cggctcgacc tcgagcgcca ccgtgagcac ggcgtcgccg gaaatgaccg
      781 cgggaagcca gcgggcctgg tgcccgtcgg tgccgaacgc ggccagcgtc tgggccgcca
      841 caacggtcgg ccacagtggc acgggggcga cccggcgacc ctgcgccagg agcgtcaggc
      901 acacctcgac caggccgagg cccgcaccgc cgtgggcctc cggcaggccg atgcccagga
      961 gtccggtggt ggccagctcc ttccagaggg cgcggtccat gcgctcgtcg gaggcctcga
     1021 cctccttgac ccgctcgagc gtggccgtcc ccgtgaacac ctgctcggcc agctcccgga
     1081 cggcctcctg ttcctcggtg aaggcgaaat ccatgtcagc gccccaccgg ctggaactgg
     1141 gggagggtca gctcgtcgtc gaaggcggtg aacacgaccg ccacgggcat gccgatctcg
     1201 acggcgtcgg cgtcgatgcc gaccacgttg ctgatgaggc gcacgccctc ctccagttcg
     1261 atcagggcca cgttgagcgg gtagtcgaag gcggggacct gcgggtagtg gttggtcacg
     1321 aagctgtaga cggtgccctg gcccgaggcc tcgatggcct cccagtccag ggactggcac
     1381 tgcgggcaca ggggcccggg cgggtggcgc agcgctccac aggacgagca cttctggatc
     1441 agcagcttct cgtccttggc cccctcgaag aagaaggcgt tgtcgaggtt cacggccggc
     1501 cggggccggt tcggcttcgg ctcggccttc ggccggggcc ggaacttgag gatccggaac
     1561 gtcatcgccc cgaccagctc gccgtcctgg tcccggtact cggtccgggt ggtgacgaag
     1621 tggccgaccc cgagaccggt cgccttctcc tccgacacgc tctcgatgac cgacgtggtg
     1681 gcgaggaggt cgcccggcac cagctcccgg aggtactcct gctcggtgtt ggtggccacg
     1741 accgacgtga acccatgctc ctcgagcagg ccgagcagct cgctctgcac gccgtcggcc
     1801 cgggggccct gggacttgag gccccgcatg gaccagacct gcaacatgac cgccggggcc
     1861 acgatcccgg gccgtccggt ggcctcggcg gccccgtcgt cgaggtacac ggggttggtg
     1921 tcccccatcg cctcgaccca gtgccggatc atgggcaggt tgaccgggtc gtcggcggtc
     1981 accgccggcc cgtagctgcg gccctcgaac gcccgcagcc gatccaggag ctcgtcggcc
     2041 gtcatcagcg ggccccccgg gtcatgccga ggcccatcca ggccacgatc tcccgctgga
     2101 tctcgttcac gccacccccg aaggtgttga tctgggccga gcgcccggcc tgctccaccc
     2161 gccccgacag gacagccccc ggcgagccct cccgcaggta gccggccgcg ccgaggacgc
     2221 cgaggagcag gtggtacaca tcgacgactg cctcggtgcc gtacaccttg accgcggacg
     2281 cgtcggccgg gttcagctcg tcgttggcca ccgcggtggt gagcttccag ttgagcaggt
     2341 gcatggcgtc gagcagggcg tggcacttgg ccaggtccgc ccgcacccac gggatggcga
     2401 gcacgccgtt ctcggcggcc cagtcgcgga cgtcggcgaa cagctcgtgg gcctcaccgg
     2461 agtgggcggc gaggccgatg cgctcgtggt tgagctgggt ggtgatc
//
LOCUS       DASTFV010000134         2733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_477698, whole genome shotgun sequence.
ACCESSION   DASTFV010000134 DASTFV010000000
VERSION     DASTFV010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477698"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VFP02_02485"
     CDS             <1..782
                     /locus_tag="VFP02_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9541919.1"
                     /translation="AAAKHLTPVTLELGGKSPAIVDRSANLEVAGRRIAWGKFLNAGQ
                     TCIAPDYVLVDETVQDELIEHIRTAVSAFYGEDPMASADYARIVNGRHFDRLSGLLGG
                     GGEAAIGGTTDADERYISPTVLVDTPADAAIMEEEIFGPLLPVLPVASVAEAIDRVNA
                     RPKPLALYVFAGDDDVVDDVLARTSSGGVCVNHTLMHITPPELPFGGVGESGQGRYHG
                     RSGFDTFSNLKSVLRKPTFPDPALLYPPYTRGKEKIVRRAL"
     gene            complement(787..2616)
                     /locus_tag="VFP02_02490"
     CDS             complement(787..2616)
                     /locus_tag="VFP02_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541920.1"
                     /translation="MVTPQRAPGPVPKAGPGLVGINLTTAGHLADALDRVAVEVDGQA
                     REARSAGLEGGQVPIAAARLALVARWAAHEAAAIRRIIDRLRAVDVTGVARWSGGRSP
                     AFADPVAGYAWGIELVDALLAGRMAEARRLLADHGRDPVVATVVVAGLGAAGTVDLLR
                     PAVAGWARPGTDADDQRAVVLGVAGLLAGAERSGTTDLTVGDLADAARRGSVPLRALA
                     LLFAGGARFPSSFVRDAVRTVVAPLNAALRAEPGLGVGPWMIPAAGAPLDARVVVLAA
                     AARDHEAAVEAVGAVDLDDLLAGSLGYLDGGVALAGVLLAATTPLTFRGTPMFDPLLT
                     TSPVHPGREGDNARRIIEWIGAHPEVPLAVHAELGHLARPWIGGFRTVGLDRVLRPPV
                     VLDEGLARAYLAYAQARDHVDEHLQDAAWSWAAGELDHLAATGATGPGFDAVGSVLGI
                     VTVTGLDADATRAAAVDRRIGRQDMLWRQVARLAVERLPPPASGLADPVVSRALDRFL
                     ASTDQELTHWRDLRDLAVAHEYLALDHLVANVVWAEDPEPIPPPPPVLLVDPDRPRLG
                     LRPPVDLDEAGVAAWTRWRSALAGHGRPARQVAGDQFLAETRD"
BASE COUNT          384 a         1006 c         1009 g          334 t
ORIGIN      
        1 aggcggcggc gaagcacctc accccggtca ccctggagct gggtgggaag tccccggcca
       61 tcgtggaccg gtcggccaac ctcgaggtcg ccggtcgccg catcgcctgg ggcaagttcc
      121 tcaatgccgg ccagacctgc atcgctcccg actacgtcct ggtcgacgag accgtccagg
      181 acgagctgat cgagcacatt cgcaccgccg tcagcgcctt ctacggcgag gacccgatgg
      241 ccagcgccga ctacgcccgc atcgtcaacg ggcgccactt cgaccgcctg tccggcctgc
      301 tggggggcgg cggtgaggcc gccatcggcg ggaccaccga cgccgacgag cgctacatct
      361 cgcccaccgt cctggtcgac acgccggccg acgccgcgat catggaggag gagatcttcg
      421 ggccgctgct gcccgtgctc cccgtggcgt cggtggctga ggccatcgac cgggtgaacg
      481 ctcgacccaa gccgctggcc ctgtacgtct tcgccggtga cgacgacgtc gtcgacgacg
      541 tgctggcgcg gacctcgtcc gggggtgtct gcgtgaacca cacgctgatg cacatcaccc
      601 cacccgagct gcccttcggc ggcgtgggcg agagcggcca gggccgctac cacgggcgca
      661 gcggcttcga caccttcagc aacctcaagt cggtcctgcg caagcccacc ttccccgacc
      721 ccgccctgct ctacccgccc tacacccggg gcaaggagaa gatcgtccgc agggcgttgt
      781 agccggtcag tcgcgggtct cggccaggaa ctggtcaccc gccacttgac gggcggggcg
      841 gccgtgtccg gccagggccg accgccaccg ggtccaggcc gcgacccctg cctcgtcgag
      901 gtcgacgggc gggcgcaagc cgagccgggg gcggtccgga tcgacgagga gcacgggggg
      961 cggtggcggg atcggttccg ggtcctcggc ccacacgacg ttggccacga ggtggtccag
     1021 cgcgaggtac tcgtgggcca cggcgaggtc gcggaggtcc cgccagtgcg tcagctcctg
     1081 gtcggtcgac gccaggaagc ggtccagtgc tctggacacg acgggatcgg ccagaccgct
     1141 cgccggcggg gggaggcgct cgacggccag acgggccact tgccgccaga gcatgtcctg
     1201 ccggccgatg cgccggtcca ccgcagccgc cctggtcgcg tcagcgtcga gcccggtgac
     1261 ggtcacgatg ccgaggacgc tgcccaccgc gtcgaacccg gggcctgtgg cgccggtggc
     1321 ggccaggtgg tcgagctcgc ccgcggccca ggaccaggcc gcgtcctgga ggtgctcgtc
     1381 gacgtggtca cgggcctggg cgtaggcgag gtaggcccgg gccaggccct cgtccagcac
     1441 gacgggagga cgcaggaccc ggtcgaggcc gacggtgcgg aaccccccga tccacggtcg
     1501 ggcgaggtgg cccagctcgg cgtgcaccgc gagggggact tcggggtggg ccccgatcca
     1561 ctcgatgatc cgccgggcgt tgtccccttc ccggccgggg tgcaccggcg aggtggtgag
     1621 caacgggtcg aacatcggcg tgccccggaa cgtcagcggc gtggtcgccg ccaggaggac
     1681 gccggccagt gccaccccgc cgtcgagata gcccagcgag ccggcgagca gatcgtcgag
     1741 atcgacggca ccgacggcct cgaccgcggc ctcgtggtct cgggcggcgg ccgccaggac
     1801 cacgacccgg gcatcgagag gcgcgcccgc cgccgggatc atccacggcc cgacgccgag
     1861 gccgggctcg gcccggagag cggcgttgag cggcgcgacc accgtccgga cggcatcccg
     1921 cacgaaggag ctcgggaatc gggccccgcc ggcgaagagg agggcgagcg cgcggagggg
     1981 aaccgaccct cggcgggcgg cgtcggccag atcaccgacc gtgagatccg tcgtcccgct
     2041 ccgttcggcc ccggcgagga ggccggcgac cccgagcacg accgcgcgct ggtcgtcggc
     2101 gtcggtcccg ggccgggccc atcccgcgac ggccggccgg aggagatcca ccgtgcccgc
     2161 ggcgccgagg ccggcgacta cgaccgtggc caccacggga tcgcggccat ggtcggccag
     2221 caggcgccgg gcctccgcca tccgaccggc gagcagggca tcgaccagct cgatccccca
     2281 cgcgtagccc gccaccgggt cggcgaacgc cgggctccgg ccaccgctcc agcgggcgac
     2341 gcccgtgaca tcgaccgcgc gcaaccggtc gatgatccgt cggatggccg ccgcctcgtg
     2401 cgcggcccag cgagccacca gggccagtcg ggcggcggcg atcgggacct ggccgccctc
     2461 gaggccggcc gagcgggcct cgcgcgcctg gccgtcgacc tcgacggcca ctcggtcgag
     2521 ggcgtcggcc aggtgacctg ccgtcgtcag gttgatgccg accaggcccg ggccggcctt
     2581 cgggaccggg cccggagctc tttgaggcgt aaccattgcg tttcatgttg tttcatgtaa
     2641 tgctcatggt caacagtcac gacgtgaagg gaacgccgcc atgccgttcg atcaggtggg
     2701 agccgatctg gggcagctgg ccacgctgcg ccg
//
LOCUS       DASTFV010000135         3988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_480344, whole genome shotgun sequence.
ACCESSION   DASTFV010000135 DASTFV010000000
VERSION     DASTFV010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480344"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /gene="hemG"
                     /locus_tag="VFP02_02495"
     CDS             <1..612
                     /gene="hemG"
                     /locus_tag="VFP02_02495"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HET9541921.1"
                     /translation="KVHSLGHVEEDGTVLALPAFATADLLADPSPGAAGLLNTIGYAS
                     PVLVTLAVPAAGIDHALDASGFLVPHTEGLLLTACSWASRKWSHLAGGDTVILRASAG
                     SAHDDRAQHLPDRELVKALRADLRRTMGLMGDPTEVRVSRWPQSLPQYAVGHLAMVAD
                     LRHTLAVEHPGLALAGSAYEGVGLPACIHDGRLAARRLLDTRG"
     gene            656..2140
                     /gene="lnt"
                     /locus_tag="VFP02_02500"
     CDS             656..2140
                     /gene="lnt"
                     /locus_tag="VFP02_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET9541922.1"
                     /translation="MTSARSGGGRRGALVSLLAGALLALAMPPWGWWPLAFVAFALFS
                     HQVDDPSRRRRAGRGALFALGWFVPAIFWMLDFTLPGYGIAVALWCGFWALAALLCPP
                     GRWRWVGLVGAAVLAEYVRWSWPFGGQPLATVAMTQVDSPLGPTVRVLGPLLLVALVV
                     TVGVALDALFAGSRRIGAGALGGVIAVVLLAGVAPDGHPTGTLDVALVQGGGPQRTRA
                     ADTDPRDVFERHLAATEDVDTPVDLVLWPENVINVEGPVETQREGEELSALARELDAT
                     LVVGAVEGEGDRFHNAALGIDADGNFTDRYEKVRRVPFGEYVPLRSFLRPFAGDALPD
                     RDAIEGEGEATVETDVGTLGVVISWEVFFANRARDAISHGGEVLLNPTNGSSYWLTIV
                     QTQQIASSRLRAMETGRWVLQAAPTGLSAIITPDGDVIDRTAVSERAVIQGTIETREG
                     LTLYTRWGDWPMVLLALGLVAGSSLAFRRRAAGTDTNRSRPDAT"
     gene            complement(2137..2574)
                     /gene="dtd"
                     /locus_tag="VFP02_02505"
     CDS             complement(2137..2574)
                     /gene="dtd"
                     /locus_tag="VFP02_02505"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HET9541923.1"
                     /translation="MRGLVQRVAEARVRVGDETVGEIGAGLCVLVGVHRDDTEAQALK
                     LADKVWGLRIMADDEGVMNRSVADTTGEVLVVSQFTLYGNTERGRRPSWLDAAPPEQA
                     EPLVDQVVSSLRDLGATVATGRFRTDMQVELVNDGPTTILLEV"
     gene            2657..3907
                     /locus_tag="VFP02_02510"
     CDS             2657..3907
                     /locus_tag="VFP02_02510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9541924.1"
                     /translation="MPADAPLRIAYLSYRGKPHVGGQGVYTRHLSKALVDLGHTVEVL
                     GGQPYPRLDERVPLVELPSLDIYNEHFPMRMPWIGELKHFADFVEVTSFSLGTFPEPL
                     AFSIRAWQHLRTRRGEFDLVHDNQSLGYGLLAIERDGLPVVATIHHPITVDRRLEMEH
                     AENGWQRWNKGRWYAFTKMQTRVASRMKRVITVSENSRDDIHKDHQVPLQNMAIVPVG
                     VDQEQFRPLPHIQRVPGRLITTASADVAMKGLAYLLEAVAKIRTERPCDLVIIGKPKE
                     EGRAMRTLEQYGLTDAVQWVYGVSDERIVELYAEAELAVVPSLYEGFSLPAIEAQCSG
                     VPLVATTGGALREVVGPDGETAFTCPPGDAAALAARINFAMDDPELATKVGLAGRQRV
                     IENWTWRHTAEKTVEQYRIRLKQG"
BASE COUNT          545 a         1522 c         1331 g          590 t
ORIGIN      
        1 aaggtccact cgctcggcca cgtcgaggag gacgggaccg tcctcgccct tcccgccttc
       61 gccacggccg acctgttggc cgaccccagc cctggcgccg ccggcctgct caacaccatc
      121 ggctacgcgt cgcccgtcct ggtcaccctc gccgtccccg ccgcgggcat cgaccacgcc
      181 ctcgacgcct ccggcttcct ggtcccccac accgagggcc tgctgttgac ggcgtgctcg
      241 tgggcctccc gcaagtggag ccacctcgcc ggcggcgaca ccgtgatcct ccgggcatcg
      301 gccggctcgg ctcacgacga ccgggcccag cacctgcctg accgcgagct ggtgaaggcc
      361 ctccgggccg acctgcgcag gaccatgggc ctgatggggg acccgaccga ggtgcgcgtc
      421 agccgctggc cccagtcgct gccgcagtac gccgtcggcc atctggcgat ggtcgccgac
      481 ctgcgccaca cgctggccgt cgagcacccc ggcctggcct tggccgggtc cgcctacgag
      541 ggcgtgggcc tacccgcctg catccacgac ggccgcctgg ccgcccgtcg cctcctggac
      601 acccgcgggt gaccccaggc acgaacctgc gtcgcccaca ccacggatct gtggtgtgac
      661 gagcgcacgt tcgggtggag ggcgccgagg ggcactcgtc agcctcctgg ccggcgcgct
      721 gctggcgctg gcgatgccgc cctggggatg gtggccgctg gccttcgtcg cgttcgcgct
      781 gttcagccat caggtcgacg acccgtcgcg tcgccggcga gccggacgcg gcgccctgtt
      841 cgccctcggc tggttcgtgc ccgccatctt ctggatgctc gacttcacgc tgcccgggta
      901 cggcatcgcc gtcgctctgt ggtgcggctt ctgggccctg gccgccctcc tctgcccacc
      961 ggggcgctgg cggtgggtgg ggcttgtcgg cgccgccgtc ctggccgagt acgtccgctg
     1021 gtcctggccc ttcggcggcc agccgctggc gaccgtggcc atgacccagg tcgactcccc
     1081 gctcggcccc acggtccggg tcctcgggcc cctgttgctg gtcgccctcg tggtgaccgt
     1141 cggtgtggcg ctggacgccc tgttcgcggg ctcccgccgc atcggcgccg gagccctggg
     1201 cggtgtgatc gccgtcgtcc tcctcgcggg ggtcgcgccc gacggccacc ccaccgggac
     1261 gctcgacgtc gctctggtcc aagggggtgg gccccagcgc acgcgggccg ccgacaccga
     1321 cccccgcgac gtgttcgaac gccatctggc cgccactgag gacgtggaca cgccggtcga
     1381 cctggtcctc tggccggaga acgtcatcaa cgtggagggc ccggtcgaga cccagcgaga
     1441 gggcgaggag ctgtcggccc tggcccggga gctggacgca accctcgtgg tcggcgccgt
     1501 cgaaggggaa ggtgacaggt tccacaacgc cgccctcggc atcgacgccg acggcaactt
     1561 caccgaccgc tacgagaagg tccggcgagt cccgttcggc gaatacgtcc ctctgcggtc
     1621 gttcctccgc cccttcgccg gcgacgcgtt gcccgaccgg gacgccatcg agggcgaggg
     1681 ggaggcaacg gtcgagaccg acgtgggcac gctcggggtg gtcatctcct gggaggtgtt
     1741 cttcgccaac cgggccaggg acgccatcag ccacggcggc gaggtcctgc tcaaccccac
     1801 caacggttcg tcctactggc tgaccatcgt ccagacccag cagatcgcct cgtcgcggtt
     1861 gcgggccatg gaaacgggtc gttgggtcct acaggccgcc cctaccggcc tgtccgccat
     1921 catcaccccc gacggcgacg tcatcgaccg caccgccgtc agcgagcgcg ctgtcatcca
     1981 gggcaccatc gagacccgcg agggcctgac cctctacacc cgctggggcg actggcccat
     2041 ggtcctcctg gcgctgggac tggtggcggg ctcctcgctc gcgttccgtc gtcgggcggc
     2101 agggaccgac accaaccgat cacggccgga cgccacctag acctcgagca ggatcgtcgt
     2161 ggggccgtcg ttgaccagct cgacctgcat gtcggtgcgg aagcggccgg tggcgacggt
     2221 ggcgcccagg tcccgcaacg acgagacgac ctggtcgacg aggggctcgg cctgctcggg
     2281 cggggcggcg tccagccacg aggggcggcg gccccgctcg gtgttgccgt agagggtgaa
     2341 ctggctgacc acgaggacct cgccggtggt gtcggccacc gagcggttca tcacgccctc
     2401 gtcgtcggcc atgatccgca ggccccacac cttgtcggcc agtttgaggg cctgggcctc
     2461 ggtgtcgtcc cggtggacgc cgacgagcac gcacaggccg gcaccgatct cgcccaccgt
     2521 ctcgtcgccg acccgaaccc gggcctccgc cacgcgctgc accagacccc gcacgaggcc
     2581 catcctggcc tgaaagtcca ccgttccggg cacacttgac cgctcagtca acggaccgga
     2641 aagcccatct cgtcacatgc ctgccgacgc gccgctgcgc atcgcctatc tcagctaccg
     2701 gggcaagccc cacgtgggtg gtcaaggcgt ctacacccgt cacctctcca aggccctggt
     2761 ggacctgggg cacaccgtcg aagtcctcgg cggtcagccc tatccccggc tggacgagcg
     2821 ggtccccctc gtcgagctcc cgagcctcga catctacaac gagcacttcc cgatgcggat
     2881 gccgtggatc ggcgagctca agcacttcgc cgacttcgtc gaggtcacgt cgttctccct
     2941 cggcaccttc ccggagccgc tggcgttcag catccgggcc tggcagcacc tgcgcacccg
     3001 ccggggtgag ttcgacctcg tccacgacaa ccagtccctc gggtacggcc tgctggccat
     3061 cgagcgggac gggctgcccg tcgtggccac catccaccac cccataaccg tcgaccgacg
     3121 gctggagatg gagcacgccg agaacggctg gcagcggtgg aacaagggtc gctggtacgc
     3181 cttcaccaag atgcagaccc gggtggccag ccgcatgaag cgcgtcatca cggtgtcgga
     3241 gaactcgcgg gacgacatcc acaaggacca ccaggtccct ctccagaaca tggccatcgt
     3301 ccccgtcggt gtggaccagg agcagttccg gccgctcccc cacatccagc gggtgcccgg
     3361 ccggctcatc accaccgcct cggccgacgt ggccatgaag ggcctggcct acctgctgga
     3421 ggccgtggcc aagatccgca ccgagcgacc ctgcgacctg gtcatcatcg gcaagcccaa
     3481 ggaggagggg cgggccatgc gcaccctcga gcagtacggg ctgaccgacg cggtccagtg
     3541 ggtctacggc gtgtccgacg agcgcatcgt cgagctctac gccgaggccg agctggccgt
     3601 ggtcccctcc ctctacgagg gcttctccct gccggccatc gaggcccagt gctccggcgt
     3661 gcccctggtg gccaccaccg gcggtgccct gcgcgaggtc gtcggccccg atggcgagac
     3721 cgccttcacc tgtccgccgg gcgacgccgc cgccctggcc gcccgcatca acttcgccat
     3781 ggacgatccc gagctggcca ccaaggtcgg cctggccggc cggcagcggg tcatcgagaa
     3841 ctggacgtgg cgccacaccg ccgagaagac ggtcgagcag taccgcatcc gcctcaagca
     3901 gggctgaccg ggtgctgacc gtcgactacg accgtctcgg cgtgcgcgcc ggtgagctcc
     3961 tgctggacct cggcgcgggc aacggtcg
//
LOCUS       DASTFV010000136         3661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_484194, whole genome shotgun sequence.
ACCESSION   DASTFV010000136 DASTFV010000000
VERSION     DASTFV010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484194"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFP02_02515"
     CDS             <1..266
                     /locus_tag="VFP02_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541925.1"
                     /translation="TFHLNNTSTITDAPTIRSLRFALQDEMDDWRDGEMLIQSLLETD
                     DEVQRAADRVAHLEKLLLAAGGIAGPGTIGDPYAEPQPTEVTA"
     gene            263..1096
                     /locus_tag="VFP02_02520"
     CDS             263..1096
                     /locus_tag="VFP02_02520"
                     /inference="COORDINATES: protein motif:HMM:NF016212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF455 family protein"
                     /protein_id="HET9541926.1"
                     /translation="MRKAFPVEDLARDDRFVRVDIQDRISDPRSVKNSRMRTQARLTP
                     DRPDSADAARALMHGIFVGEIQALEGAGRTCWDFDTGDGRDEAPFALKLDMARQCWDE
                     ARHCEISVKLSDWMGTEIGEFSEGAFLYAAACNPDPVLRLTGVNRALEGLAIDVFNTM
                     REFGEMAGDPVLEFCEDWMLADEVTHVKMGSDWLRRLTEDDPERRKDALEFQRVVDKL
                     FSLGGFRSDDDDSPVKLARRFRELAGFDDEEIDEIADLASEALEEARTRQEAAAALAT
                     A"
     gene            1101..1658
                     /locus_tag="VFP02_02525"
     CDS             1101..1658
                     /locus_tag="VFP02_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541927.1"
                     /translation="MAQIELRPDKWAMVDYDPDRLRAIAEAMANAAGLPADLEVVVAL
                     DDRSPLGRYSIDSMDPVLVSMESGAFEDPKHPRQLSEGGAAGVLGRLFFRVADRLTEG
                     FADAPDDADLEPAAGVAWEVHAAGRLARAGQQAQLQRWRYHFRNRHGFTDEGDAAFDT
                     LWSADALSWAEVQALSAAALAGIPA"
     gene            1688..2173
                     /locus_tag="VFP02_02530"
     CDS             1688..2173
                     /locus_tag="VFP02_02530"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET9541928.1"
                     /translation="MTEAEHALDELLGGINRVMWILTAADAGERSGCLMGFATQSSLD
                     PPRFLACVSRANHTFALAARAEHVAVHAAPDEADLTLARLFGEETGDEIDKFARCEWH
                     EGPEGQPVLDACPAWFVGRVLDRIDLGDHVGLLLEPVAVEGTPTDVLTFARAQDLDAA
                     H"
     gene            complement(2184..2504)
                     /locus_tag="VFP02_02535"
     CDS             complement(2184..2504)
                     /locus_tag="VFP02_02535"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET9541929.1"
                     /translation="MPDPDDVDLTVVVMAFTAADPEALLSVLAKYVVLARGHPGCRNI
                     DLIASVTHPGRLLVIQKWSSPAAQQAHFDSADMVEMAEACRGLLTAPPDIDLLEAISA
                     HDLT"
     gene            2716..3651
                     /locus_tag="VFP02_02540"
     CDS             2716..3651
                     /locus_tag="VFP02_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9541930.1"
                     /translation="MPKERVERDEEDLVRLYLTDIGQYPLLTKDDEVRLAQQIEGGTA
                     AREEMDAATTLTPARKRELRKALRLGEDAERTFVQSNLRLVVSIAKKYQASGLPLLDL
                     IQEGNLGLMHAVEKFDWRKGFKFSTYATWWIRQAITRGIANTGRTIRLPVHAGDTLAR
                     LQKARSRLELKYGRPATLSELAAEVEMPEDKVTEALRFAAEPLSLSEPLREDGDAELG
                     DVVEDRSAESPFEVAATALLPEEINRLLAPLDEREREILKLRFGLDRGEPRTLEEVGE
                     HFNLTRERIRQIEARAMSQLRHPSSDTGARDLLAV"
BASE COUNT          563 a         1352 c         1248 g          498 t
ORIGIN      
        1 acacgttcca cctgaacaac acgtccacga tcaccgacgc gcccaccatc cgttcgctgc
       61 gcttcgcgct gcaggacgag atggacgact ggcgcgacgg cgagatgctc atccagtcct
      121 tgctcgagac cgacgatgaa gtacaacggg ccgccgatcg ggtggcccac ctggagaagc
      181 tgttgttggc cgcgggcggc attgccggtc cgggcaccat cggcgacccg tacgctgaac
      241 cccagcccac ggaggtgacg gcatgaggaa ggcctttccg gtcgaggacc tggcccggga
      301 cgatcgcttc gtccgggtcg acatccagga ccgcatcagc gacccccgct cggtgaagaa
      361 cagccgcatg cggacccagg cccggctgac gccggaccgc ccggactcgg ccgatgccgc
      421 ccgggccctc atgcacggca tcttcgtcgg ggagatccag gccctcgagg gcgccgggcg
      481 cacctgctgg gacttcgaca ccggcgacgg ccgggatgag gcccccttcg ccctgaagct
      541 ggacatggcc cgacagtgct gggacgaggc ccgccactgc gagatctcgg tcaagctcag
      601 cgactggatg ggcaccgaga tcggcgagtt ctccgaggga gcgttcctct acgccgcggc
      661 ctgcaacccg gacccggtcc tgcgcctcac cggcgtgaac cgggcgctgg agggcctggc
      721 catcgacgtc ttcaacacca tgcgggagtt cggcgagatg gccggcgacc ccgtcctcga
      781 gttctgcgag gactggatgc tggccgacga ggtcacccac gtgaagatgg gctcggactg
      841 gctccgtcgt ctcaccgagg acgaccccga gcgccggaag gacgccctcg agttccagcg
      901 ggtggtcgac aagctcttct ccctcggcgg cttccgcagc gacgacgacg acagcccggt
      961 caagctggcc cgccgcttcc gcgagctggc cggcttcgac gacgaggaga tcgacgagat
     1021 cgccgacctc gccagcgaag ccctcgagga ggcccgcacc cgccaggagg cggcggccgc
     1081 cctcgccacc gcctgacccg gtggcccaga tcgagctccg ccccgacaag tgggcgatgg
     1141 tcgactacga ccccgaccgc ctccgggcca ttgccgaggc catggccaac gcggccggcc
     1201 tcccggcgga cctggaggtc gtggtcgcgc tggacgaccg gtcgcccctg ggccgctaca
     1261 gcatcgactc gatggatccc gtgctggtgt ccatggagag cggcgccttc gaggatccca
     1321 agcacccccg tcagctctcc gagggtgggg ccgccggtgt cctcggccgc ctcttcttcc
     1381 gggtggccga ccggctgacc gagggcttcg ccgacgcgcc cgacgacgcc gacctcgagc
     1441 cggcggccgg cgtggcctgg gaggtccatg ccgccgggcg cctggcccgg gccggccaac
     1501 aggcccagct ccagcggtgg cgctaccact tccggaaccg gcacgggttc accgacgagg
     1561 gcgacgccgc cttcgacacc ctctggtccg ccgacgcgct cagctgggcc gaggtccagg
     1621 ccctctcggc agccgcgctc gccggcatcc ccgcctgacc gtccgggcgc cggggtaggg
     1681 accggcgatg accgaagcgg agcacgcgct ggacgagttg ctcggcggca tcaaccgggt
     1741 gatgtggatc ctgaccgccg ccgacgctgg tgagcggtcc ggctgcttga tgggcttcgc
     1801 cacccagtcc agcctcgatc cgccccggtt cctggcctgc gtctcccggg ccaaccacac
     1861 cttcgccctc gccgcccggg ccgagcacgt cgccgtccac gccgcccccg acgaggcgga
     1921 cctcaccctg gcccggctct tcggcgagga gacgggcgac gagatcgaca agttcgcccg
     1981 ctgcgaatgg cacgagggcc cggagggcca acccgtcctc gacgcctgcc ccgcctggtt
     2041 cgtcggccgg gtgctcgacc gcatcgacct cggcgaccac gtcggcctgc tcctcgagcc
     2101 cgtcgccgtc gaagggaccc cgaccgacgt gctcaccttc gcgcgggccc aggacctcga
     2161 cgccgcccac tgaaggcgag cgcctacgtc aggtcgtgtg cgctgatggc ctcgagcagg
     2221 tcgatgtcgg ggggcgcggt gagcaggccg cgacaggcct cggccatctc caccatgtcg
     2281 gccgagtcga agtgggcctg ttgggcggcg ggcgacgacc acttctggat gaccagcagc
     2341 cggcccgggt gggtgaccga cgcgatcagg tcgatgttgc gacagcccgg gtgcccccgg
     2401 gccaggacca cgtacttcgc caggaccgag agcagcgcct cgggatcggc cgcggtgaag
     2461 gccatgacca caacggtcag gtcgacgtcg tcggggtcgg gcacgggtgg gacctcaagc
     2521 acggtggggt tcgggccgtt gtcacggaga gcgaccgggc caccccggtc ggcggtcgac
     2581 gccggttggg ctacttgacc gcgtcggtat cgtaggcaac gccccactgg cgaccgcggg
     2641 attcggtccc ggccgccggt accgtccatc gaggttccca ctgaaggttc ccgaatctgg
     2701 ccagggagat cgtcgttgcc caaggaacgt gtcgagcggg acgaagaaga cctcgttcgc
     2761 ctgtacctca ccgacatcgg gcagtacccg ctgctcacca aagatgacga ggtccggctc
     2821 gcgcagcaga tcgagggtgg caccgccgcc cgcgaggaga tggacgcggc cacgacgctg
     2881 acgcccgccc gcaagcggga gctgcgcaag gcccttcgcc tcggtgagga cgcggaacgc
     2941 accttcgtgc agtccaacct gcgcctggtc gtctccatcg ccaagaagta ccaggcctcg
     3001 ggcctgcccc tgctggacct gatccaggag ggcaacctcg gcctgatgca cgcggtcgag
     3061 aagttcgact ggcgcaaggg cttcaagttc tccacgtacg ccacctggtg gatccgccag
     3121 gccatcaccc gcggcatcgc caacaccggc cgcaccattc gcctgccggt gcacgccggc
     3181 gacaccctcg cccgcctgca gaaggcgcgc tcccggctcg agctcaagta cggccgtccg
     3241 gccaccttgt ccgagctggc cgccgaggtg gagatgcccg aggacaaggt caccgaggcg
     3301 ctgcgcttcg ccgccgagcc cctgtcgctg tccgagcccc tgcgggagga cggtgacgcc
     3361 gagctgggtg acgtggtcga ggaccgctcc gcggagtccc ccttcgaggt ggcggccacc
     3421 gcgctgctcc ccgaggagat caaccggctg ctggccccgc tcgacgagcg cgagcgcgag
     3481 atcctcaagc tgcgcttcgg gctcgaccgc ggcgagccgc gcacactcga agaggtcggc
     3541 gagcacttca acctcacacg cgagcggatc cgccagatcg aggcccgggc catgtcgcag
     3601 ctgcgccatc ccagcagcga cacgggggcc cgcgacctgc tcgctgtctg acccaccaac
     3661 g
//
LOCUS       DASTFV010000137         2483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_489038, whole genome shotgun sequence.
ACCESSION   DASTFV010000137 DASTFV010000000
VERSION     DASTFV010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489038"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VFP02_02545"
     CDS             complement(<1..365)
                     /locus_tag="VFP02_02545"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET9541931.1"
                     /translation="MQIAIPLFDRLTALDAIGPYEVLQRLPGANVTFVGTRTGEVRTE
                     NGFLGLTVDAHVDEVPQPDVIVVPGGVGSRQAVDDEALVDWIRAAHETTTFTTSVCTG
                     SFVLGAAGLLDGLTATTHW"
     gene            complement(392..1351)
                     /locus_tag="VFP02_02550"
     CDS             complement(392..1351)
                     /locus_tag="VFP02_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9541932.1"
                     /translation="MHRVVVVAFPGLQPLDVVGPHEVFAGANRGLDPAQRYEIEVVAR
                     RTGPVEGESGLALHAAALGATEGIGTLLLPGGGGVVAASEDPVLVDWIRQAADGAERV
                     ATVCSGTFLAGAAGLIDGRRVTTHWARAEQLAARHPRAVVDADPIHLRDGDLWTSAGV
                     TAGIDLALALVEEDHGPALAQTIARWLVMFLRRPGGQSQFAVPVWAEPAPPGPVRQAQ
                     DLVHAEPGADWRVDDLARRVGMSPRHFSRSFTDQVGQSPGHYVTTVRVEAARRLLEEE
                     PAATTALVARRCGFGTAESLRRAFLRRLGVPPEQYRRRFATNP"
     gene            complement(1458..>2483)
                     /locus_tag="VFP02_02555"
     CDS             complement(1458..>2483)
                     /locus_tag="VFP02_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541933.1"
                     /translation="SPDGSVVVRGVIRADGSTRLTWLDPVDGRPIARVRLDEPASLRV
                     VAADGRVAVGPPDVPVTGLIYPVARAQSPVSVVAPDGTVHTYTLPGSVEPEAFSTDGS
                     GLFVLSYTPAGNPTEYQVNRLDLESGEIGGVYGQDSADERQERMGGTARTQVWAPDGS
                     TVYTLYTQEVDGQATSFIHVLNLDEGWAFCLDLPEPLGWDTPAALAVAPDGGRLWVTE
                     AAGHGAVVDTDTLEVLATGDFPSPGAYGLAAVADGDRVFVGSESEIAVLDGIDLHQLA
                     RWRTTRPIIGLRRDDLDGRLVAVTDGQLTTFPLDAPERDPAGRDLPYTSPLVVIDPTL
                     PTAECAC"
BASE COUNT          324 a          836 c         1002 g          321 t
ORIGIN      
        1 ctccagtggg tggtggcggt gaggccgtcg agcaggccgg cggcgccgag gacgaaggat
       61 cccgtgcaca cgctggtggt gaaggtggtc gtctcgtggg cggcgcggat ccagtcgacc
      121 agtgcttcgt cgtccaccgc ctgccggctg ccgaccccgc cggggaccac gatcacgtcg
      181 ggctggggga cctcgtccac gtgggcgtcc acggtcaagc cgaggaagcc gttctcggtg
      241 cggacctcac cggtgcgggt gcccacgaag gtcacgttgg cgccggggag gcgttggagc
      301 acctcgtagg gcccgatggc gtcgagggcg gtgaggcggt cgaagagggg gatggcgatc
      361 tgcacggctg gctccttggt tcggtggtcg gtcaggggtt ggtggcgaaa cggcggcggt
      421 actgctcggg gggcacgccg aggcgacgca ggaaggcccg ccgcaggctc tcggccgtgc
      481 cgaagccgca gcgtcgggcc accagcgccg tggtcgccgc cggctcctcc tcgagcaggc
      541 ggcgggcggc ctcgacccgc acggtcgtga cgtagtggcc gggcgactga ccgacctggt
      601 cggtgaagga gcggctgaag tgccgcgggc tcatgcccac ccggcgggcc aggtcgtcga
      661 cccgccagtc ggccccgggc tcggcgtgga cgaggtcctg ggcctggcgc accggtcccg
      721 gtggggcggg ctcggcccag acggggacgg cgaactggga ctggccgccc ggtcgccgca
      781 ggaacatgac cagccaccgg gcgatggtct gggccaacgc cgggccgtgg tcctcctcca
      841 ccagggcgag ggcgaggtcg atgcccgcgg tgaccccggc cgaggtccag aggtcgccgt
      901 cccgcaagtg gatggggtcg gcgtcgacca cggctcgggg gtgacgggcg gccagctgct
      961 cggctcgggc ccagtgggtg gtgacccgcc ggccgtcgat caacccggcg gcgccggcca
     1021 ggaaggtccc gctgcacacg gtggcgaccc gttcggcccc gtcggcggcc tggcggatcc
     1081 agtcgaccag gacgggatcc tcgctggcgg ccacgacgcc cccaccaccg gggaggagca
     1141 gcgtgccgat gccttccgtg gccccgaggg cggcggcgtg gagggccagg ccgctctcgc
     1201 cttcgaccgg cccggttcga cgggcgacga cctcgatctc gtagcgctgg gccggatcga
     1261 ggccgcggtt ggccccggcg aacacctcgt gaggcccgac gacgtcgagg ggctggagcc
     1321 cggggaaggc gacgaccacg acgcggtgca cggggcgagg gtgcctcacc gccgcgatgg
     1381 cgtcaatgac gatatcccca cctttcctgc cacgaccgag ccccctggcg agggcccctt
     1441 cgggagttga gctcagctca gcaggcgcac tcggcggtgg ggagggtggg gtcgatgacc
     1501 accagggggc tggtgtaggg caggtcccgg ccggcggggt cccgttcggg ggcgtcgagc
     1561 gggaaggtcg tgagctgccc gtcggtcacc gccaccaggc gtccgtcgag gtcgtcgcgc
     1621 cgcagcccga tgatcgggcg agtggtgcgc caccgggcca gctggtgcag gtcgatgccg
     1681 tcgagcacgg cgatctcgct ctccgaaccg acgaagaccc ggtcgccgtc cgccaccgcg
     1741 gcgaggccgt aggcgcccgg cgacgggaag tcaccggtcg ccaggacctc gagggtgtcg
     1801 gtgtcgacca cggccccgtg gccggcggct tcggtcaccc agaggcggcc accgtcgggg
     1861 gccacggcca acgccgcagg agtgtcccaa ccgaggggct cgggcagatc gaggcagaag
     1921 gcccagccct cgtccaggtt caggacgtgg atgaaggagg tggcctgccc gtcgacctcc
     1981 tgggtgtaga gcgtgtagac ggtgctgccg tcgggggccc acacctgggt gcgggccgtg
     2041 ccgcccatgc gctcctggcg ctcgtcggcg ctgtcctggc catagacgcc gccgatctcg
     2101 cccgactcga ggtcgaggcg gttgacctgg tactcggtgg ggttgccggc gggggtgtag
     2161 ctgaggacga agagcccgct gccgtcggtg ctgaaggcct ccggctccac gctgcccggg
     2221 agggtgtagg tgtgcacggt cccgtcgggg gcgacgacgc tcaccgggct ctgggcccgg
     2281 gcgacgggat agatgaggcc ggtcaccggg acatcgggcg gtccgacggc gacccggccg
     2341 tcggcggcca cgacccgcag gctggcgggc tcgtcgaggc ggacccgggc gatgggacgg
     2401 ccgtcgacgg ggtccagcca ggtcaggcgg gtggagccgt cggcccggat gacaccccgg
     2461 accaccaccg aaccgtccgg tga
//
LOCUS       DASTFV010000138         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_491669, whole genome shotgun sequence.
ACCESSION   DASTFV010000138 DASTFV010000000
VERSION     DASTFV010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491669"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /gene="jag"
                     /locus_tag="VFP02_02560"
     CDS             <1..1016
                     /gene="jag"
                     /locus_tag="VFP02_02560"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HET9541934.1"
                     /translation="VRRRGRSVEWVQTSGKTIDEAKERALDELGVDDRDAEFEVLEEP
                     RSGLFGLTRGDAKVRARVRPTSPRPKQERGRRRSGGGGRERSRSGGGNGGGRSRSSRG
                     GGNGGGRSRAEASDRDKDQDRKQDDDRERGGSGGGNGGGRGGGRSRGNDGGGRNRSSR
                     NEGGRERSRDNDRPKEDKGEPMTEASVQEQAAAAEEFLSGLVEAFGLTADVSSEEVDE
                     AVEVRIDGNDLGLLIGPKGRTLWALQELTKTVVQRQQGGGLSTRIRVDVAGYRQRRKA
                     ALERFTQDAAAAVLDSGQARALEPMGAADRKVVHDTVNDIEGVVTRSEGEDPRRRVVI
                     APE"
     gene            1030..1617
                     /locus_tag="VFP02_02565"
     CDS             1030..1617
                     /locus_tag="VFP02_02565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HET9541935.1"
                     /translation="MLTEAQGLGWIGQGPLEDQLRHAGAFTQLLGEGPGVVFDLGSGG
                     GLPALPCALSAPRWTWVLVESRLRRADHLVSACRRLELADRVDVVHGRAEDVGRDPAY
                     RTTADVVTARAFGPPAVVAECAAPLLRVGGRLLVSEPPDGVDRWPEDGLAQVGLSSAV
                     HRVAEGFAIAELGQVAACPDRYPRRAGRGVRSPLF"
     gene            2022..>2549
                     /locus_tag="VFP02_02570"
     CDS             2022..>2549
                     /locus_tag="VFP02_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9541936.1"
                     /translation="MGAKEYQPDLTIPLPKPRNPRVIAVANQKGGVGKTTTAVNLGAG
                     LAERGLRVLVVDLDPQGNASTGLGLNIRELDSTMYDVLLDDVPIEDCIEPSEVRNLFV
                     APASLDLAGAEIELVSAMSRELRLRTALAPVKDDYDYVLIDCPPSLGLLTLNAFAAVE
                     EVLVPIQCEYYALEGL"
BASE COUNT          374 a          857 c          948 g          370 t
ORIGIN      
        1 ccgtaagaag aagaggtaga tccgtggagt gggtacagac cagcggcaag acgatcgacg
       61 aggccaagga acgggccctc gacgaactcg gtgtcgacga ccgtgatgcc gagttcgaag
      121 tcctggaaga gccccgcagc ggcctgttcg ggctgacccg cggcgacgcc aaggtccgcg
      181 ctcgggtccg gccgacctcg ccgcggccca agcaggagcg gggtcgccgt cgttcgggcg
      241 gcggcggccg cgagcgcagc cgttcgggag gcggcaacgg cggcggccgc tcccgcagca
      301 gtcggggtgg tggcaacggc ggcggccgct cccgcgccga ggcctccgac cgggacaagg
      361 atcaggaccg caagcaggac gatgaccgcg agcgcggcgg ttcggggggc ggcaacggcg
      421 ggggccgcgg tgggggccgg tcccggggca acgacggggg tggccgaaac cggtcctctc
      481 ggaacgaggg cggccgtgag cggtcccgtg acaacgatcg acccaaggaa gacaaaggtg
      541 aaccgatgac cgaagcgagt gtccaggagc aggcggcggc ggcggaggag ttcctctccg
      601 ggctggtcga ggccttcggg ctgaccgccg acgtgagctc ggaggaggtc gacgaggccg
      661 tcgaggtgcg catcgacggc aacgacctcg gtctgctgat cggccccaag ggccgcacgc
      721 tgtgggccct gcaggagctg accaagaccg tggtccagcg ccagcagggc ggtggcctct
      781 cgacccgcat ccgggtggac gtggccggct accggcagcg ccgcaaggcc gccctggagc
      841 ggttcacgca ggatgcggcg gcggccgtgc tggattcggg ccaggcccgg gccctcgagc
      901 ccatgggggc ggccgaccgg aaggtcgttc acgacaccgt caacgacatc gagggcgtcg
      961 tcacccgctc cgagggcgag gatccccgtc ggcgggtcgt gatcgcccct gaatgagacg
     1021 ctgaccgccg tgctgaccga ggctcagggc ctcggttgga tcggtcaggg tcccctcgag
     1081 gaccaacttc gtcacgccgg ggccttcacc cagttgctgg gtgagggccc cggtgtcgtt
     1141 ttcgacctgg gcagcggggg cggactgccc gccctcccct gtgccctctc ggcaccgagg
     1201 tggacctggg tcctggtgga gagccggctc cgacgggctg atcacctggt ctccgcctgt
     1261 cggcgtttgg agttggctga ccgggtcgac gtcgtccacg gtcgagcgga ggacgtcggc
     1321 cgggatccgg cgtaccggac caccgccgat gtcgtgaccg ctcgggcgtt cggccccccg
     1381 gccgtcgtgg cggagtgcgc ggctcctctg ctccgggtcg gcggccggct tctcgtgagc
     1441 gagccgcctg acggcgtcga ccgttggccg gaggacggct tggcacaggt cgggctctcc
     1501 tcggcggtcc accgggtggc ggagggcttc gccatcgccg agctcgggca ggtagcggcg
     1561 tgcccggatc gctatccccg gcgggccggc cgtggggtcc ggtcgccgct gttctgaagc
     1621 gtttcccgtg aaaccttttc ggttggcggg cggcctgtgt ttcccgggaa accccttccc
     1681 acggcccagg atccgtaacg ggtcggtggc ctccttcgga ctcggtccat gaccgccacg
     1741 aacgtctgag ctaccccgcg gccgggtgat ccaccgggac aagacatggt cggctggcgt
     1801 gatccaaccc cttcgtcgcc tcatcgcgga gctcgaggcg ttcggatcgg agccctactg
     1861 cccgcagggc cgtcggcgca tgatggtggt gaacgggaag ctcgtgcgca tcgacggagg
     1921 tcgagggtcc ttgcgtttgg gtggccgaac aggaaggatg ggcgccgatg gcgaggaccc
     1981 gtggtcgtaa tcgcgcccgt gcggaggacg acgatgaacg ggtgggagcc aaggagtacc
     2041 agcccgacct gacgatcccc ctgcccaagc cccggaatcc tcgggtcatc gccgtggcca
     2101 accagaaggg cggggtgggc aagacgacca ccgcggtcaa cctgggcgcc ggcctggccg
     2161 aacggggcct gcgggtcctc gtggtcgatc tcgaccctca gggcaacgcc agcaccggcc
     2221 tcggcctgaa catccgcgag ctcgactcca cgatgtacga cgtgctgctg gacgacgtgc
     2281 ccatcgagga ctgcatcgag cccagcgagg tccgcaacct cttcgtggcc cccgccagcc
     2341 tggatctggc cggcgccgag atcgagctgg tctccgccat gtcccgggag ctgcggctgc
     2401 gcacggcctt ggccccggtg aaagacgatt acgactacgt cctcatcgac tgcccaccgt
     2461 ccctcggcct gctcaccctc aacgccttcg ccgcggtgga ggaggtcctg gtccccatcc
     2521 agtgtgagta ctacgccctc gagggcctc
//
LOCUS       DASTFV010000139         3822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_491747, whole genome shotgun sequence.
ACCESSION   DASTFV010000139 DASTFV010000000
VERSION     DASTFV010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491747"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VFP02_02575"
     CDS             <1..376
                     /locus_tag="VFP02_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541937.1"
                     /translation="ESSAVDASPNGADGSFADGSTEYDAHADLSVGVESDGRFAPAQA
                     DVPPTPEPAEASPILGASNDAAEVARQLSNLSPAAARAVAAAAKATTDEEREAALAAV
                     AQEGDEPIDRELLLRFLASVKS"
     gene            392..1084
                     /locus_tag="VFP02_02580"
     CDS             392..1084
                     /locus_tag="VFP02_02580"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HET9541938.1"
                     /translation="MDAAIVVGVAVLGVLVSPFLLRLIVQVPAMATVGYVSDEASPIT
                     PTIRERAVQVLTPAVFVLAGLRWGASAVLVPFLLLAAVLVVVSMIDLEHFRIPDRIVF
                     PALAMSAALIIFVSLIEGFDVVFVRNAFIGSIAYFTLLLVPHLIYPRGMGFGDVKLAL
                     LMGLFLGWIYPDPFQVLGLVMWALVLGSGLGVLAGVGFALLRGRRAEFPFGPALALGC
                     LLAIQFSDALVG"
     gene            1176..2351
                     /gene="aroC"
                     /locus_tag="VFP02_02585"
     CDS             1176..2351
                     /gene="aroC"
                     /locus_tag="VFP02_02585"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET9541939.1"
                     /translation="MLRYLTAGESHGKALVVVIEGLPAGLPILEADLQAELARRRLGF
                     GRGPRMRFEQDELQLLTGIRHGRTLGSPVAIEIGNSEWERKWTEEMSPAPGETAAPLT
                     QPRPGHADLAGMQKYGFTDARDVLERASARETAARVAAGAIAKLLLSHLGVSVLSHVV
                     RMGDAVASGVRPGADELERVDASEVRCFDPDAESAMVDAIKAAAKAGDSLGGTVEVLG
                     FGVPVGLGSHVHWDRKLDALLAQALMSIQAVKGVAIGDGFDVAAKRGSEAHDPISWDE
                     DAGAYQRASDKAGGIEGGISTGGLLVAQVAMKPLATLNRPTLKTVDTETKEETVSFKE
                     RTDVTAVPAMGVVAETMTALVLAREATRKFGGDSLDEFVANANAYLASVRKRALGDG"
     gene            2360..2872
                     /locus_tag="VFP02_02590"
     CDS             2360..2872
                     /locus_tag="VFP02_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET9541940.1"
                     /translation="MPKHVVLVGMMGAGKSAVGRRVAARLVRPFSDSDTVVEDRVGRT
                     VGEIWRHDGEAAFRRLEAEALQESLADPTPAVIAAAGGTVMDAGSRAALLADDAVVIW
                     LRADPAILLGRVRHGDHRPLLEDDPAGVLARLNDERAALYDEVSDVVIDVDDLDLDEV
                     AARVLEEIDG"
     gene            2869..>3822
                     /locus_tag="VFP02_02595"
     CDS             2869..>3822
                     /locus_tag="VFP02_02595"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HET9541941.1"
                     /translation="MIRVPVPLPDQPYDVLVGAGARHRLLEVLPVGAERAAVVTQADI
                     GVPVDPGVEHEVFLLDDGEEAKSLESVEELCRRFSRWGLTRRDVVVTVGGGVVTDTAG
                     FAAAVYHRGVRVVHVPTTLLGQVDAAIGGKTGVNLPEGKNLVGAFWQPSAVLCDTEVL
                     ATLPPREYTSGLGEMAKYHFLTGIDMAGLPLDERVAECVRIKAELVVRDATEHGDRAL
                     LNYGHTLGHALEIAGTYDLRHGEAVAIGLVFAACLARGLERIDDDRVAEHRRVIEGYG
                     LPTTIDGGLDPGALVDLMRRDKKAFDGLTFVLDGPRGPEIVT"
BASE COUNT          500 a         1382 c         1373 g          567 t
ORIGIN      
        1 cgagagctcg gccgtggacg cctcgccgaa cggggccgac ggctccttcg ccgacggctc
       61 gacggagtac gacgcccacg ccgacctctc ggtgggcgtc gagtccgacg gtcggttcgc
      121 cccggcgcag gccgacgtcc cgccgacgcc ggagcccgcg gaggccagcc cgatcctcgg
      181 cgccagcaac gacgcggccg aagtggcccg ccagctgtcg aacctgagcc cggctgcggc
      241 ccgagccgtg gcggcggcgg ccaaggcgac caccgacgag gagcgggagg cggccctcgc
      301 ggccgtggcc caggagggcg acgagcccat cgaccgggag ctgctgctcc ggttcctcgc
      361 ttcggtcaag tcctagccct acattcaccc gatggacgcc gccatcgtgg tcggagtcgc
      421 cgtcctcggc gtgctcgtga gtcccttcct gctgcgcctg atcgtgcagg tcccggcgat
      481 ggccaccgtg gggtacgtca gcgacgaggc gtcaccgatc accccgacca tccgggagcg
      541 ggcggttcag gtcctcacgc cggcggtgtt cgtcctcgcc gggctccggt ggggcgccag
      601 tgcggtcctg gtgccgttcc tgctcctggc ggccgtactg gtggtcgtgt cgatgatcga
      661 cctcgagcac ttccgcatcc ccgaccggat cgtcttcccg gcgctggcca tgtcggcggc
      721 cctgatcatc ttcgtctcgc tcatcgaggg cttcgacgtc gtgttcgtgc gcaacgcctt
      781 catcggatcg atcgcctact tcacgctcct gttggtgccc cacctgatct acccgagggg
      841 catgggcttc ggcgacgtga agctggccct gctcatgggc ctctttctcg gctggatcta
      901 ccccgatccg ttccaggtcc tggggctggt catgtgggcc ctggtgctcg gtagcggcct
      961 gggtgtcctg gccggcgtgg gcttcgccct cctgcgaggc cgccgggccg agttcccctt
     1021 cggccccgcc ctggccctcg gctgccttct ggccatccag ttcagcgacg cgctcgtcgg
     1081 ctgagacgag atggaacgat cgttccatct cggctccgtg tcgcctgagc ggctaagacc
     1141 ggctcccgac tcaacccggc ccgtatcgtg ggcgggtgct ccggtacctg accgctggtg
     1201 agtcccacgg caaggccctc gtcgtcgtca tcgagggcct ccccgccgga ctgcccatcc
     1261 tggaggccga tctccaggcc gagctggctc gccgccggct gggcttcggc cgcggtcccc
     1321 gcatgcgctt cgagcaggac gagctccagc tgctgaccgg catccgccac gggcggaccc
     1381 tgggctcgcc ggtcgccatc gagatcggca actcggagtg ggagcggaag tggaccgagg
     1441 agatgtcgcc ggctccgggt gagacggccg ccccgctgac ccagccccga cccggccacg
     1501 ccgacctggc gggcatgcag aagtacgggt tcaccgacgc ccgggacgtc ttggaacggg
     1561 cctcggcccg ggagacggcg gcgcgcgtcg ccgccggggc catcgccaag ctgctgctgt
     1621 cgcaccttgg cgtgtcggtc ctgtcccacg tcgtgcgcat gggtgacgcc gtcgcctccg
     1681 gcgtccggcc tggcgccgac gagctcgagc gggtcgacgc ctccgaggtc cgctgcttcg
     1741 accccgatgc cgagtcggcc atggtcgacg ccatcaaggc cgcggccaaa gcgggcgact
     1801 ccctcggcgg caccgtcgaa gtgctgggct tcggggtgcc cgtcgggctg ggcagccacg
     1861 tccattggga ccgcaagctc gacgccctgc tggcccaggc cctcatgagc atccaggccg
     1921 tgaagggcgt ggccatcggc gacggtttcg acgtcgccgc gaagcggggg agcgaggccc
     1981 acgaccccat ctcctgggac gaggacgccg gggcgtacca gcgagcgtcg gacaaggcgg
     2041 gcgggatcga gggcggcatc tccaccggcg gcctgctcgt ggcgcaggtg gccatgaagc
     2101 cgctcgccac cttgaaccgg cccacgctca agacggtgga caccgagacc aaggaggaga
     2161 cggtctcctt caaggaacgg accgatgtca ccgccgtgcc cgccatgggc gtcgtggccg
     2221 agacgatgac cgctctggtg ctggctcggg aggccacccg caagttcggc ggcgactcac
     2281 tggacgagtt cgtggccaac gccaacgcct atctggcgtc ggtccgcaaa cgggccctgg
     2341 gcgacggctg atgggcgccg tgccgaagca cgtggtgctg gtcgggatga tgggcgcggg
     2401 caagagcgcc gtcggtcgcc gggtggccgc tcgcctggtc cgaccgttca gcgacagcga
     2461 cacggtcgtc gaggaccggg tcggacggac ggtgggcgag atctggcgcc acgacggtga
     2521 agccgcgttc cgccggctcg aggccgaggc cctccaggag tcactggccg accccacgcc
     2581 cgccgtcatc gccgcggccg ggggcacggt catggacgcc gggagccggg ccgctctgct
     2641 cgccgacgac gctgtcgtca tctggctgcg ggccgacccc gccatcctgc tcggacgcgt
     2701 ccgccatggt gaccaccggc ccctgctcga ggacgacccg gccggcgtgc tggcacggct
     2761 gaacgacgag cgggcggcgc tctacgacga ggtgtccgac gtggtcatcg acgtggacga
     2821 cctcgacctc gacgaggtcg cggcccgggt gctcgaggag atcgacgggt gatccgggtc
     2881 ccggtcccgt tgcccgacca gccctacgac gtgctggtgg gagccggggc ccgtcaccgc
     2941 ctgctcgagg tcctccccgt cggggccgag cgggccgcgg tcgtgaccca ggccgacatc
     3001 ggcgtacccg tcgaccccgg cgtcgagcac gaggtcttcc tcctcgacga cggcgaggag
     3061 gccaagagcc tggagagcgt cgaggagctc tgccgccgct tctcccgctg gggcctcacc
     3121 cgccgggacg tggtcgtgac ggtgggtggg ggcgtggtca ccgacaccgc cgggttcgcg
     3181 gccgccgtct accaccgggg cgtgcgggtg gtgcacgtgc ccaccacgct gctgggccag
     3241 gtcgacgccg ccatcggggg caagacgggc gtgaacctgc ccgagggcaa gaacctggtc
     3301 ggcgccttct ggcagccctc ggcggtgctc tgcgacaccg aggtcctggc cacgctgccg
     3361 ccccgggagt acacctccgg cctgggtgag atggccaagt accacttcct gaccggcatc
     3421 gacatggccg gcctgcccct cgacgagcgg gtggccgagt gcgtgcgcat caaggccgag
     3481 ctggtcgtgc gggacgccac cgagcacggc gaccgggccc tgctcaacta cggccacacc
     3541 ctgggccacg ccctggagat cgccgggacc tacgacctgc gccatggcga ggcggtggcc
     3601 atcggcctcg tgttcgccgc ctgcctggcc cggggcctgg agcggatcga cgacgaccgg
     3661 gtggccgagc accgcagggt gatcgagggc tacggcctgc ccacgacgat cgacggcggc
     3721 ctggaccccg gtgcgctggt cgacctcatg cgtcgcgaca agaaggcgtt cgacggcctg
     3781 accttcgtgc tcgacggacc ccggggccct gagatcgtca cc
//
LOCUS       DASTFV010000140         4737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_500493, whole genome shotgun sequence.
ACCESSION   DASTFV010000140 DASTFV010000000
VERSION     DASTFV010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500493"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFP02_02600"
     CDS             <1..332
                     /locus_tag="VFP02_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541942.1"
                     /translation="LIIFVSVYEMGDYLVGSGSKNAIEGPLAGFAAIAVFAFALWVVT
                     FVPFRDNALLAFGALAAGLCPLGQLMGSAVLPRSDAPASALRRLDSLLVLGPAWVVAL
                     WSYTEII"
     gene            349..1107
                     /locus_tag="VFP02_02605"
     CDS             349..1107
                     /locus_tag="VFP02_02605"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HET9541943.1"
                     /translation="MEIFSNFMDPGGGGEMLGRYVHFLAGITWIGMLYYFNFVQTPAF
                     AEVSDATRSEAMRTITWRALWWFRFGALLTFLSGVWILGAQHLLGDRFDDFWTTSSGL
                     SILIGALFATTMFLNVWLVIWPAQQVIIGSARKVAEGGEADPEAAARAKPAARASRAN
                     TLMSIPMLWFMGFTSHYSFNYGETDDSSFIVVLVLFLIILAVIELTALGKIGGYDSEL
                     AKTLFDKHKNTIIAGFVVWFVLFVIGYELIIGAS"
     gene            1133..1669
                     /locus_tag="VFP02_02610"
     CDS             1133..1669
                     /locus_tag="VFP02_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541944.1"
                     /translation="MSASLDDFSDPNLLDGLTGWPLDRVRAFRDEAQAAEGGLSYVRR
                     VVQGRLDIVGAELQRRREGGDPGDLSGLIAQLPDLLADPDRPPGAGARSPRRLDLAEV
                     PDDLAVELDQIVDTDALADLPGFDTEALDKMAQRLVDFERWISERRHVVQGVVDTLQA
                     EIARRYRDGEATVDGLLD"
     gene            1672..2067
                     /locus_tag="VFP02_02615"
     CDS             1672..2067
                     /locus_tag="VFP02_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9541945.1"
                     /translation="MPSGDELKGEIETRWRDLGEFIREQRNLGQLSLRKLSEKAEVSN
                     PYLSQIERGLRHPSAEILRQIARALEISSEELYVRAGILEERDDRPDLVAEIRRESDL
                     TEDQKKHLIYMYESFRSANAGAVEAEAAE"
     gene            2086..3309
                     /locus_tag="VFP02_02620"
     CDS             2086..3309
                     /locus_tag="VFP02_02620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9541946.1"
                     /translation="MTLSNGVTRAAVLSMHTSPLAQPGTGDSGGMNVYVRELVAALSQ
                     AGVECCVFVRRSDNESPDVVDVEPGFRVVHVDAGPVDLAKEDLPEILPAYIAGVEEWL
                     RREPVDVIHANYWLSGLAGHELKHRLDLPLVSTFHTLARVKAENGDPEPERRAEAEAS
                     IIGCSDVICASSTTEESQLAVLYDAPADRIEIVPPGVDHAFFSPGDQRGARSALGLGE
                     EPVLLFVGRLQPLKRLGMAVDVLARIERQDATLVVVGGPSGTQGEAELARVRDQAASL
                     GVADRIRWVAPVPHHLLSTYYRAADVCLVPSRSESFGLVALEAAACGIPVVAAAVGGL
                     RSIVEHGRTGFLVEGGDPAYFGAYVDEVLGSPRLAAELGRVAAERGRTFTWSTTAARL
                     RRLYADLATRAPVSC"
     gene            3334..3798
                     /locus_tag="VFP02_02625"
     CDS             3334..3798
                     /locus_tag="VFP02_02625"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HET9541947.1"
                     /translation="MLSADALDALEDRIDAWFAREAAANPMIAAVDRGEPGERRWYVR
                     VRGEQKDTFTLWFTLGQRTLRYETYVMPAPEENRAAFYEHLLARNLGLYGWGFAIGEE
                     SAVFLTGALGVEALAGDEALARELDRVLGSGWEYVERCFRPALRIGFASKVQ"
     gene            4036..>4737
                     /locus_tag="VFP02_02630"
     CDS             4036..>4737
                     /locus_tag="VFP02_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541948.1"
                     /translation="MPARVQRGAAVLLAMLALAVAAPVWAQSADDLDENQQQQDELDS
                     QLEILEADHAELEAALEATSARVRQQEAAKRAAEQRLEAARAEVRAAQAAVAEMQAEI
                     VELGHQADEDAVDLYMNPDPTLTVMDTDDLTQATRRDALLSTIASRHSDVLDRLGGLE
                     IDLVDAEARTREAAAAVEAERAEVAALLADYQATLRQQQRLESALTARIDAVEAEAAA
                     LAEEEASIRAALAQAS"
BASE COUNT          647 a         1753 c         1610 g          727 t
ORIGIN      
        1 ccctcatcat cttcgtgtcc gtctacgaga tgggcgacta cctcgtcggc tccggctcga
       61 agaacgccat cgagggcccc ctggccggct tcgccgccat cgccgtcttc gccttcgccc
      121 tctgggtcgt cacgttcgtg ccgttccggg acaacgcgtt gctggccttc ggagcgctgg
      181 ccgccgggct ctgccccctc ggccagctga tgggatcggc cgtcctgccc cggtccgacg
      241 cccccgcgtc ggccctgcgc cgcctggatt ccctgctcgt cctgggcccc gcgtgggtcg
      301 tcgccctctg gtcctacacc gagatcatct gaactagtct ccgcggcgat ggagatcttc
      361 agcaacttca tggatccggg cggtggcggc gagatgctcg gtcgctacgt ccacttcctg
      421 gccggcatca cctggatcgg gatgctctac tacttcaact tcgtccagac cccggcgttc
      481 gccgaggtca gcgacgccac ccgctccgag gccatgcgca cgatcacgtg gcgggccctg
      541 tggtggttcc gcttcggcgc cctgctcacc ttcctgtcgg gcgtctggat cctcggtgcc
      601 caacacctgc tcggggaccg cttcgacgac ttctggacca catcgagcgg cttgtcgatc
      661 ctcatcggtg ccctgttcgc caccaccatg ttcctcaacg tgtggttggt catctggccc
      721 gcccagcagg tcatcatcgg ctcggcccgc aaggtggccg aaggcggcga ggccgatccc
      781 gaggccgccg cccgggccaa gcccgcggcc cgggcctccc gggccaacac gctgatgtcg
      841 atccccatgc tgtggttcat gggcttcacc agccactact ccttcaacta cggcgagacc
      901 gacgacagct cgttcatcgt cgtgctcgtc ctgttcctca tcatcctggc cgtgatcgag
      961 ctgaccgccc tgggcaagat cggcggctac gacagcgagc tggccaagac actcttcgac
     1021 aagcacaaga acacgatcat cgccgggttc gtggtgtggt tcgtgctgtt cgtcatcggc
     1081 tacgagctca tcatcggcgc ctcctaggaa tcggcgccgt acgctcgacc ccgtgtctgc
     1141 gtcgctcgac gacttctccg atcccaacct gctcgacggc ctgaccggct ggcccctgga
     1201 ccgcgtccgg gccttccgtg acgaggccca agcggccgag ggggggcttt cctacgtccg
     1261 ccgggtggtg cagggccgcc tcgacatcgt cggggccgag ctccagcgcc gccgggaggg
     1321 cggtgacccg ggcgacctca gtggcctgat cgcccagctg cccgacctcc tggccgaccc
     1381 cgatcgcccg cctggcgcgg gcgcccggtc cccccgccgc ctcgacctgg ccgaggtccc
     1441 cgacgacctg gccgtcgagt tggaccagat cgtggacacc gacgcgctgg ccgacctccc
     1501 cggcttcgac accgaggccc tcgacaagat ggcccagcgg ctggtcgact tcgaacgctg
     1561 gatctccgag cgtcgccatg tcgtgcaggg cgtcgtcgac acgctccaag ccgagatcgc
     1621 ccggcggtac cgcgacggcg aggccaccgt cgacgggctc ttggactgac cgtgccctcg
     1681 ggtgacgagc tcaagggcga gatcgagacc cgctggcgcg acctcggcga gttcatccgg
     1741 gagcagcgca acctgggcca gctctcgctg cgcaagctct cggagaaggc cgaggtctcc
     1801 aacccgtacc tgagccagat cgagcggggc ctgcgccacc cctcggcgga gatcctgcgc
     1861 cagatcgccc gggccctcga gatctcctcc gaagagctct acgtgcgggc cggcatcctc
     1921 gaggagcggg acgaccgccc ggacctggtc gccgagatcc gccgggagag cgacctcacc
     1981 gaggaccaga agaagcacct catctacatg tacgagtcct tccgcagcgc gaacgccgga
     2041 gcggtagagg ccgaggcagc cgagtaggga atcggggacc tgcccgtgac gttgtccaac
     2101 ggcgtgaccc gcgccgccgt gctctccatg cacacgtctc ccctggccca gccgggcacg
     2161 ggcgacagcg gcgggatgaa cgtgtacgtg cgggagctgg ttgcggccct cagccaagcc
     2221 ggcgtcgagt gctgcgtgtt cgtgcgccgc agcgacaacg agtcccccga cgtggtcgac
     2281 gtcgagcccg ggttccgggt ggtccacgtc gacgccggcc ccgtcgacct ggccaaggag
     2341 gacctgcccg agatcctccc ggcctacatc gccggcgtgg aggagtggct gcggcgcgag
     2401 ccggtcgacg tgatccacgc caactactgg ctgtcggggc tggccggcca cgagctgaag
     2461 caccgcctcg acctgcccct ggtgtcgacc ttccacaccc tggcccgggt gaaggccgag
     2521 aacggcgatc cggagcccga gcgccgggcc gaggccgagg cctcgatcat cggctgctcc
     2581 gacgtcatct gcgcctccag caccaccgag gagagccagc tggccgtcct ctacgacgct
     2641 ccggccgatc gcatcgagat cgtgccgccg ggcgtggacc atgccttctt ctcgcccggt
     2701 gaccagcggg gggcccgcag cgccctcggg ctgggggagg agccggtgct gctcttcgtc
     2761 ggccggctcc agcccctcaa gcgcctcggg atggcggtcg acgtgttggc ccgcatcgag
     2821 cgccaggacg ccacgctggt cgtcgtcggg ggcccgtcgg gcacgcaggg cgaggccgag
     2881 ctggcccggg tccgggacca ggcggcgagt ctcggcgtcg cggatcgcat ccgctgggtc
     2941 gcgcccgtgc cccaccacct gctgtccacc tactaccggg ccgcggacgt gtgtctggtc
     3001 ccgagccgct cggagtcctt cggcctggtg gccctcgagg ccgcggcctg tggcatcccc
     3061 gtggtcgccg ccgccgtcgg gggcctgcgt tccatcgtgg agcacggccg caccggcttc
     3121 ctggtcgagg ggggcgaccc cgcgtacttc ggggcctacg tggacgaggt cctgggcagt
     3181 ccgcgccttg ccgccgagct ggggcgggtc gcggccgagc gggggcgcac gttcacctgg
     3241 tcgaccacgg cggcccggct ccgccgcctc tacgccgacc tggccacccg ggccccggtc
     3301 agctgctgag tgtccggccc cggacccgcc ggcgtgctgt cggcggacgc cctcgacgcc
     3361 ctcgaggacc gcatcgacgc ctggttcgcc cgggaagcgg ccgccaaccc catgatcgcc
     3421 gccgtcgacc ggggtgagcc gggagagcgt cggtggtacg tccgggtccg gggcgagcag
     3481 aaggacacct tcacgctgtg gttcacgctg ggccagcgca ccttgcgcta cgagacctac
     3541 gtgatgccgg cgcccgagga gaaccgcgcc gccttctacg agcacctgtt ggcgcgcaac
     3601 ctcggcctct acggctgggg cttcgccatc ggcgaggagt cggcggtgtt cctgaccggt
     3661 gccctgggcg tcgaggccct cgccggcgac gaggcgctgg cccgcgagct ggaccgggtc
     3721 ctcggctcgg gctgggagta cgtggagcgg tgcttccgtc ccgccctgcg gatcggcttc
     3781 gccagcaagg ttcagtaagt ttcaatcatt tatattgtat ttccaggtct aaggctctag
     3841 ggtcggctac gacccggtgt gcccggcgtc gtcgccccgt cggggaagcg ggggtggtcg
     3901 tgagcggcac accgggtcgc attacggtcc tgttacagtc gggggccgat ccggtcctcc
     3961 tccggagcgg tcccggcgga ctactctccg gaaccggacg ggtcgaccgg tccgacaccc
     4021 cagtccagag gattcatgcc cgcacgcgta caacgaggtg ccgccgtcct gctggcgatg
     4081 ctcgccctcg ccgtggcggc ccccgtgtgg gcccagtccg ctgacgacct ggacgagaac
     4141 cagcagcagc aggacgagct cgactcccag ctcgagatcc tggaggcgga ccacgccgag
     4201 ctggaagcgg ccctggaggc caccagtgcc cgggtccgcc agcaggaggc ggccaagcgg
     4261 gcggccgagc agcgcctcga ggccgctcgg gccgaggtcc gcgccgccca ggccgcggtg
     4321 gccgagatgc aggccgagat cgtcgagctg ggtcaccagg ccgacgaaga cgccgtcgac
     4381 ctctacatga accccgatcc gacgttgacg gtgatggaca ccgacgacct gacccaggcc
     4441 acccgccggg atgccctgct gtcgaccatc gcctcccgcc acagcgacgt gctcgaccgg
     4501 ctcggcggcc tcgagatcga cctggtcgac gccgaggccc ggacccgcga ggccgccgcc
     4561 gcggtcgagg ccgagcgggc cgaggtcgcg gccctcctgg ccgactacca ggccacgctc
     4621 cgccagcagc agcgactgga gtcggcgctg accgcccgga tcgacgccgt cgaagccgag
     4681 gccgcggccc tggccgagga ggaggcctcc atccgggccg ccctggccca ggcgtca
//
LOCUS       DASTFV010000141         4637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_503618, whole genome shotgun sequence.
ACCESSION   DASTFV010000141 DASTFV010000000
VERSION     DASTFV010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503618"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VFP02_02635"
     CDS             <1..1029
                     /locus_tag="VFP02_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET9541949.1"
                     /translation="PGDFDKRTLLCNTGAEAVEAAVGIARYATGRQGVVVFEGAYHGR
                     SNLTLAMTSKFGLFKKGFGPFAPEIYRLPFPDLYRRPSQFTEDEWVAHAVSQLDRAMV
                     AQVDPSHVAAVVIEPVLGEGGFVPAPAPFLQRLRELCDQHGMVLIADEIQAGFGRTGT
                     LFAIEHAGVVPDMVTTAKSLAAGMPLAAVTGRAELMDAPHPGGLGGTYSGNPLACVAA
                     IEAIDTIREPAFLARAVAVGDRIRGHLDAIQAEHPDLVGDVRGLGAMLVMELVTDAES
                     KAPDPDATVAVTLATLERGVITIRAGLYSNCVRFLPPLTISDEEIDEAMTVVAEAVRV
                     AAKERAHG"
     gene            1022..2104
                     /locus_tag="VFP02_02640"
     CDS             1022..2104
                     /locus_tag="VFP02_02640"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HET9541950.1"
                     /translation="MADQPIRVLDLVGSPAEVGRVHGAAYAVEIREYTDERVHLSSDG
                     VWAGRAVTHDEVIALAEACLPAHEAYDPELYAEMLAMAEGAGVSPAEAVIVGGFTDFV
                     DTVRASGGDPAPEDNCTAALVPLDAGGGEPWFAQTWDMHDTATPYIVMLRTRVTGRPE
                     ALVFTTVGCLGQIGMNEVGLCVGINNLTAADGKIGVTWPFVVRKALAQTSIDDALACI
                     TGADLAGGHNFLLLDAEGRGYDVEAMPTATHVIELHDEPVVHTNHCVAEITQAVQGPR
                     APVRIASTENRYARAVELLGKRPIDETTLMALTRDEEAICERPEPPYEVMSCGATVMR
                     PRARELWAVWGLPVENQYEHFTVGRE"
     gene            2187..2801
                     /locus_tag="VFP02_02645"
     CDS             2187..2801
                     /locus_tag="VFP02_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9541951.1"
                     /translation="MNLTADLAPACHRLEISAFPSADPAGLLSEEDIRVFAEIFPEGF
                     FVALDGDEPVGQGAGIFVDFDFATQVQHTITSLCGPHQCDNHDPEAHWYYGVDIVVSA
                     DHRGRGIGRKLYDLRKDLVQRHDKRGIIAGGDLPGYPEHAGAMDIETYVAKVVAGELT
                     DPTLSMQLHNGFEVRGVIHDYLPHEHGEDHASLIVWENPDYSGG"
     gene            2806..4233
                     /locus_tag="VFP02_02650"
     CDS             2806..4233
                     /locus_tag="VFP02_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9541952.1"
                     /translation="MLLPDLVLEADPDARALTFEDESWTFGRLASELSRVAAAVTAST
                     APGDRVALVGRNRLAYVAALYGIPAAGRIAVPLNHRLAPAEIAAQVERAGATLVIADD
                     LPGAVPWAAWEAEVAAAHPGPRSAALVEDDPAWILFTSGTTGTAKGAVLTHASLLAAV
                     RGANAARPVADDDVYLFCFPLCHVAAYNVACLHAAGRPVVLMAGFDAATLPRLVARYG
                     VTSLSLAPTMLVMLLDDPSLTPEAFASVRQVAYGAASMAPDLLRRGTEILDVGFAQGY
                     GMTELSGNAVFLDEEGHRAGLGDRPHLLRAAGRPSPQAEVRIAADGEILVRGPQVMAG
                     YWDDPEATAATIVDGWLHTGDIGEVDDEGYLYVVDRAKDIVVTGGENVSSREVEDVLA
                     DHPGVATVAVIGVPDERWGEAVCAVVVPVPGAEPTLEDLVAFTRGRLGGFKQPRRLVL
                     VDSLPVNTAGKVLKRELREQVTADA"
     gene            4226..>4637
                     /locus_tag="VFP02_02655"
     CDS             4226..>4637
                     /locus_tag="VFP02_02655"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET9541953.1"
                     /translation="MPDARLLVVWHSNSGHTQEMVDLVLAGCRDPEVGVIDVRDLPAL
                     EATADDVRWATGVILGTPAHFGYMSGALKHFFDTVYLDLLDQTRGLPYALFVKGGNDT
                     DGAVAAIHKIVTGLAWKEVQPPLTVVGDLTDDQRA"
BASE COUNT          659 a         1696 c         1620 g          662 t
ORIGIN      
        1 cccggggact tcgacaagcg caccctgctc tgcaacacgg gggccgaggc ggtcgaggcc
       61 gcggtcggca tcgcccgcta cgccacgggc cgccagggcg tggtcgtgtt cgagggggcg
      121 taccacggtc ggtcgaacct gaccctggcc atgaccagca agttcgggct gttcaagaag
      181 ggcttcggcc ccttcgcccc ggagatctac cggttgccgt tccccgatct ctaccgccgc
      241 cccagccagt tcaccgagga cgagtgggtg gcccacgccg tcagccagct ggaccgggcg
      301 atggtggccc aggtcgaccc gtcgcacgtg gccgccgtcg tcatcgagcc ggtcctgggc
      361 gagggggggt tcgtccccgc cccggctccg ttcctgcagc ggctgcggga gctgtgcgac
      421 cagcacggca tggtcctgat cgccgacgag atccaggccg ggttcggtcg gaccggcacg
      481 ctcttcgcca tcgagcacgc cggcgtcgtc cccgacatgg tcaccacggc caagtccctc
      541 gccgcgggga tgcccctggc cgccgtcacc ggtcgggccg agctgatgga cgccccccac
      601 ccgggtggcc tcggggggac ctactccggc aatccgctgg cgtgcgtcgc cgccatcgag
      661 gccatagaca ccatccgcga gcccgccttc ctggcccggg ccgtggccgt gggcgaccgg
      721 atccgtggtc acctggacgc gatccaggcc gagcaccccg acctcgtcgg cgatgtgcgg
      781 ggactggggg cgatgctggt catggagctg gtgaccgacg ccgagtccaa ggcccccgac
      841 cccgacgcca ccgtggcggt gaccttggcc acgctggagc ggggcgtgat caccatccgg
      901 gccggcctct actcgaactg cgtgcgcttc ctgccgccgc tgacgatcag cgacgaggag
      961 atcgacgagg cgatgaccgt ggtggccgag gccgtgcggg tggcggccaa ggaacgagcc
     1021 catggctgat cagccgatcc gggtcctcga cctcgtcggc tcaccggcgg aggtgggtcg
     1081 ggtgcacggg gcggcctatg cggtcgagat tcgggagtac accgacgagc gggtgcacct
     1141 gtcctccgac ggggtgtggg ccggccgcgc cgtgacgcac gacgaggtca tcgccctggc
     1201 cgaggcctgc ctgccggctc acgaggccta cgaccccgag ctctacgccg agatgctggc
     1261 catggccgaa ggggccggcg tgtcccccgc cgaagccgtc atcgtcggcg gcttcaccga
     1321 cttcgtcgac acggtgcggg cgtcgggtgg ggacccggcg ccagaggaca actgcacggc
     1381 cgcgctggtc cccctggacg ccgggggcgg cgagccctgg ttcgcccaga cctgggacat
     1441 gcacgacacg gcgacgccct acatcgtgat gctccgcacc cgggtgaccg ggcggcccga
     1501 ggcgctggtg ttcaccaccg tcggttgcct cggtcagatc gggatgaacg aggtcggcct
     1561 ctgtgtcggc atcaacaacc tcaccgcggc cgacggcaag atcggcgtga cctggccctt
     1621 cgtggtccgg aaggcgctgg cccagacgtc catcgacgac gccctggcct gcatcaccgg
     1681 ggccgacctg gccggtggcc acaacttcct gctcctcgac gccgaaggtc ggggctacga
     1741 cgtcgaagcc atgccgacgg ccacccacgt catcgagctg cacgatgagc ccgtggtcca
     1801 caccaaccat tgcgtggccg agatcaccca ggccgtccaa gggccgcggg ccccggtgcg
     1861 catcgcctcc acggagaacc gctacgcccg agccgtcgaa ctgttgggca agcgtcccat
     1921 cgacgagacg accctcatgg ccctgacccg ggacgaggag gccatctgcg agcggcccga
     1981 gccgccgtac gaggtcatgt cctgcggggc caccgtcatg cgcccgaggg cgcgtgagct
     2041 gtgggcggtg tggggcctgc ccgtcgagaa ccagtacgag cacttcaccg tcggccgtga
     2101 gtgatcggcg gagccggtcg gcgggccgag taggcccgca agccgatgag cgacagcgaa
     2161 gaggcctcga cacagcgatc cggtacgtga acctcacggc ggacctggcg ccggcctgtc
     2221 accgcctgga gatctcggcg ttcccttccg cggatcccgc cggcctcctg agtgaggagg
     2281 acatccgcgt cttcgccgag atcttcccgg agggcttctt cgtggccctc gacggtgacg
     2341 agcccgtcgg ccagggggcg gggatcttcg tcgacttcga cttcgcgacc caggtccagc
     2401 acacgatcac ctcgctgtgc gggccccacc agtgcgacaa ccacgatccg gaggcccact
     2461 ggtactacgg cgtagacatc gtggtctcgg ccgaccaccg tggccgcggg atcggccgga
     2521 agctctacga cctgcgcaag gacctcgtcc agcgccacga caagcgcggg atcatcgccg
     2581 gaggtgacct gcccggctac cccgagcacg ccggggccat ggacatcgag acctacgtcg
     2641 ccaaggtggt ggcgggcgag ctgaccgacc cgaccttgtc catgcagctc cacaacggct
     2701 tcgaggtccg gggcgtgatc cacgactacc tgccccacga gcacggcgag gaccacgcca
     2761 gcctcatcgt ctgggagaac cccgactact ccggcggctg agcccgtgct cctgcccgac
     2821 ctcgtcctgg aggcggaccc ggacgcgagg gccctgacct tcgaggacga gtcctggacc
     2881 ttcgggcgtc tggcctcgga gctttcgcgg gttgcggccg cggtgacggc ctcgacggca
     2941 ccgggcgacc gagtggccct ggtcggccgc aaccggctgg cctacgtcgc ggccctctac
     3001 ggcatcccgg ccgcggggcg catcgccgtg cccctgaacc accgccttgc gcccgccgag
     3061 atcgccgccc aggtcgagcg ggcgggggcc acgctcgtca tcgccgatga cctccccggg
     3121 gctgtgccct gggcggcctg ggaggccgag gtggccgcag cccaccccgg gccacggtcg
     3181 gcggccctcg tcgaggacga tccggcctgg atcctcttca ccagcggcac gaccgggacc
     3241 gcgaaggggg ccgtcctgac ccacgccagc ctgctcgccg cggtccgggg agcgaacgcg
     3301 gctcggccgg tggccgacga cgacgtctac ctgttctgct tcccgctctg ccacgtcgcc
     3361 gcctacaacg tggcctgcct gcatgccgcg ggccgccccg tggtcctcat ggccgggttc
     3421 gacgccgcca ccctcccccg cctcgtggcc cggtacggcg tcacctcgct ctccctggcc
     3481 cccacgatgc tggtgatgtt gctggatgat ccgagcctca cccctgaggc cttcgcctcc
     3541 gtgcgacagg tggcctacgg cgcggcgtcc atggcccctg acctgctgcg gcggggcacc
     3601 gagatcctgg acgtcggctt cgcccagggc tacggcatga ccgagctgtc aggcaacgcc
     3661 gtcttcctcg acgaggaggg gcaccgggcg gggctcggcg accggcccca cctcttgagg
     3721 gcggcgggtc ggcccagccc acaggccgag gtccggatcg ccgcggacgg tgagatcctg
     3781 gtccgcggcc cgcaggtcat ggccggctac tgggacgacc ccgaggcgac cgcggcgacc
     3841 atcgtcgacg gctggctcca cacgggcgac atcggcgagg tcgacgacga gggctacctg
     3901 tacgtcgtcg accgggccaa ggacatcgtc gtcaccgggg gcgagaacgt gtcgtcccga
     3961 gaggtcgaag acgtcctcgc cgaccacccg ggcgtggcca cggtggccgt gatcggcgtg
     4021 cccgacgagc gctgggggga ggccgtgtgt gctgtggtcg tccccgttcc cggggccgag
     4081 cccaccctcg aggacctggt ggcgttcacc cggggccggt tgggcggctt caagcagccg
     4141 cgccgcctgg tcctggtcga cagcctgccg gtgaacaccg cgggcaaggt cctcaagcga
     4201 gagctccgcg agcaggtgac cgcggatgcc tgacgcccga ctgctcgtcg tctggcactc
     4261 gaactccggc cacacccagg agatggtcga cctcgtcctg gccggctgcc gggatcccga
     4321 ggtcggcgtc atcgacgtcc gcgatctgcc tgccctggag gccaccgctg acgacgtgcg
     4381 gtgggcgacc ggcgtgatcc tggggacacc ggcgcacttc gggtacatga gtggggccct
     4441 caagcacttc ttcgacacgg tctacctgga cctgctggat cagacccggg ggctccccta
     4501 cgccttgttc gtgaagggcg gcaacgacac cgacggagcc gtcgccgcca tccacaagat
     4561 cgtgaccggc ctggcctgga aggaggtcca gcccccgctc accgtggtcg gtgacctcac
     4621 cgacgaccag cgcgccg
//
LOCUS       DASTFV010000142         2354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_506230, whole genome shotgun sequence.
ACCESSION   DASTFV010000142 DASTFV010000000
VERSION     DASTFV010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506230"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="VFP02_02660"
     CDS             <1..1221
                     /locus_tag="VFP02_02660"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541954.1"
                     /translation="HRLLSLSFAQFQSDRALVRAERGIDGRRRELEEVEATATCDRGD
                     VDEYLALLDAGRVAQAQAPQRVIEEALSGLSPGDVVDAPMKDGHERVAVLSVAHRAQG
                     HVRVRAVSRTGKVLTFGAHTVTAAPVVLGALELPEPYRPNNQKFRNEVARRLDRTRTR
                     RPSRSTREVVAPDDHPVAGCPDLRKHLRSARRARRLRQEIAAHEKRLGQETGSLAREF
                     DRVLQVLDGRGILDGWALTESGLRLARLYHECDLLVAEAIGDGLLDGLDGPDLVALLS
                     CFTYEHRSPEPAPAPTFPDVALRDRWEQLADLATDLLKEEAALSLRLTREPDPGFIPL
                     AYEWTKGDQLDEVLGDDELSGGDFVRQIRQLLDLLRQIAEVAPDPTTRDTARAAIRRV
                     DRGVVAASSSVSVT"
     gene            1218..1829
                     /locus_tag="VFP02_02665"
     CDS             1218..1829
                     /locus_tag="VFP02_02665"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541955.1"
                     /translation="MTVEKGRDWGSAGALPEDGVIVRSDAEARAVVTAARRAGEEVPP
                     LGLLGGDLCRTVGGRGDEARLRSNVAARLNVDLGSVLMDGRLHWFVAHLVTRRGWLRG
                     PLFGAFNAQWLGPWDVAPRAHPGDGQLEIVEVTMSAGDRLKARSRLATGTQVPHPGIR
                     IRRADALQWDVPKGHAVWLDGERVGPATTLSIRVEPDGLRVVV"
     gene            1839..>2354
                     /locus_tag="VFP02_02670"
     CDS             1839..>2354
                     /locus_tag="VFP02_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9541956.1"
                     /translation="MDVDTVKDRLAQEVERRADVLLEISHSIHEHPELCYEEHHAHDL
                     LAGVLEDEGFDVERGAAGMDTAFVAKAGTGDGPTIGVFCEYDALPEIGHACGHNVIGA
                     AGLGAGLAAATVAEELGGRVVVLGSPAEEGGGGKVFLEERGALDGLDAAMMIHPADGD
                     LQAMNVIAIQQV"
BASE COUNT          315 a          877 c          849 g          313 t
ORIGIN      
        1 caccgcttgc tgtcgctgtc gttcgcccag ttccagtccg accgggccct ggtccgggcc
       61 gagcggggca tcgacgggcg gcgccgcgag ctggaggagg tcgaagccac cgcgacctgc
      121 gaccggggcg acgtcgacga gtacctggcc ctcctcgacg ccggccgggt ggcgcaggcc
      181 caggcacccc agcgggtcat cgaggaagcg ttgagcggtc tcagtccggg cgacgtggtc
      241 gacgccccca tgaaggacgg ccacgagcgg gtcgccgtcc tgtcggtggc ccaccgggcc
      301 cagggccatg tccgggtccg agccgtcagc cggacgggca aggtcctgac gttcggcgcg
      361 cacacggtca ccgccgcgcc ggtggtgctg ggcgcactcg agctgcccga gccctatcgg
      421 ccgaacaacc agaagttccg caacgaggtc gcccgccgcc tcgaccggac ccgcacccgt
      481 cggcccagcc gctcgacccg cgaggtcgtc gcgcccgacg accacccggt ggcgggatgc
      541 cctgatctgc gcaagcacct gcgctcggcc cgccgggccc gccggctgcg tcaggagatc
      601 gccgcccacg agaagcggct cggccaggag accggctcgt tggcccggga gttcgaccgg
      661 gtgctccagg tcctggacgg ccgcgggatc ctggacggct gggccctgac cgagtccggg
      721 ctccggctcg cccggctgta ccacgagtgc gatcttctcg tggccgaggc catcggtgac
      781 ggcctgctcg acggcctcga cggaccggac ctggtcgccc ttctgtcctg cttcacctac
      841 gagcaccgca gccccgaacc ggcacccgcc ccgacctttc ccgacgtcgc cctgcgggac
      901 cggtgggagc agctggccga cctggccacc gacctgctca aggaggaggc cgcgttgtcc
      961 ttgcgcctca ctcgtgaacc ggatcccggt ttcatcccgc tggcctacga atggacgaag
     1021 ggggaccagc tcgacgaggt cctgggcgac gacgagctgt ccggcgggga cttcgtgcgc
     1081 cagatccgcc agctgctgga cctgttgcgt cagatcgccg aggtcgctcc tgaccccacg
     1141 acccgggaca cggcccgcgc cgccatccgc cgggtcgacc ggggggtggt cgccgcctcc
     1201 tccagcgtca gcgtgacatg accgtcgaga agggccggga ctgggggagc gcgggagcgc
     1261 tgcctgagga cggggtgatc gtgcgctccg acgccgaggc ccgggccgtg gtcaccgccg
     1321 cccggcgggc aggggaggag gtcccgccgc tcgggctcct cggcggtgat ctctgccgca
     1381 ccgtcggagg gcgaggcgac gaggcccgcc tccgctcgaa cgtcgcggcc cggctgaacg
     1441 tggacctggg ctcggtcctg atggacggcc gcctgcactg gttcgtcgcc cacctcgtga
     1501 cccgccgcgg ctggctccgg ggacccctct tcggtgcgtt caacgcccaa tggctcgggc
     1561 cctgggacgt cgcccctcgc gcccaccccg gtgacggcca gctggagatc gtcgaggtca
     1621 cgatgtccgc cggcgaccgg ctgaaggccc gctcccggct ggccaccggc acccaggtgc
     1681 cccatcccgg catccgcatc cggcgggccg acgctctcca gtgggacgtc cccaagggcc
     1741 acgccgtctg gctcgacggc gagcgcgtcg gcccggccac gaccctgtcg atccgggtcg
     1801 agcctgacgg gctgcgcgtc gtcgtctaga caggggcgat ggacgtcgac acggtcaagg
     1861 accggctggc ccaggaggtc gagcgccggg ccgacgtgct gctggagatc tcccacagca
     1921 tccacgagca ccccgagctc tgctacgagg agcaccacgc ccacgacctg ctggccgggg
     1981 tcctcgaaga cgagggcttc gacgtcgagc ggggcgcggc cggcatggac accgcgttcg
     2041 tggccaaggc cggcaccggc gacggcccga ccatcggtgt cttctgtgag tacgacgcgc
     2101 tgccggagat cggccatgcc tgtggccaca acgtcatcgg cgccgccggc ttgggcgccg
     2161 gcctggccgc cgccaccgtg gccgaggagc tcggcggccg ggtcgtcgtc ctcggctcac
     2221 cggccgagga ggggggtggg ggcaaggtct tcctggaaga gcgaggggcc ctcgacggcc
     2281 tcgacgcggc gatgatgatc caccccgccg acggggacct gcaggccatg aacgtcatcg
     2341 ccatccagca ggtc
//
LOCUS       DASTFV010000143         2940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_507887, whole genome shotgun sequence.
ACCESSION   DASTFV010000143 DASTFV010000000
VERSION     DASTFV010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507887"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VFP02_02675"
     CDS             complement(<1..598)
                     /locus_tag="VFP02_02675"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9541957.1"
                     /translation="MVDASMVSFPVNGTEADGYLSTPDGGSGPGLLVIQEWWGLVPQI
                     KAVCDRLAGEGFTALAPDLFHGDFAEHTEMDKAGELMNSLPPERAAKDMAGAIAHLLA
                     SDAVTGVRVGVIGFCMGGLLTLMISALEGDRVAAAVTFYGAPLGDGEPDWSGLTAKVE
                     GHFAENDDFFPPEAVRDLESRLQAMGKDVSFTIYPGTGH"
     gene            complement(666..1307)
                     /locus_tag="VFP02_02680"
     CDS             complement(666..1307)
                     /locus_tag="VFP02_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HET9541958.1"
                     /translation="MPSLHSFATFALASFLLVIVPGPSVLFVISRGVALGRRAALATV
                     IGNAAGVYVQALLVAVGLGAVISRSATVFNAIKLVGAGYLMYLGIQAVRHRRALSEVL
                     DAAGTVRPTKHLLREGFVVGLANPKAAVFFAAILPQFVEPGGAPAGLQMAVLALVSVG
                     VALVSDGAWGLAAGSARVWLSSSPRRLERLGGAGGITMIGLGVGLAFSGGRKA"
     gene            1367..2755
                     /locus_tag="VFP02_02685"
     CDS             1367..2755
                     /locus_tag="VFP02_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9541959.1"
                     /translation="MSTHPYAERFAVNRRLPESGRQEGELLDELQQMATEEDAYWETG
                     KCSGTMYCGDHEHYAFMGEAFQLFAHANILQRDMFPSATKLEGEIIAMTLDLMHGAEA
                     EGGDAVGMVTTGGTGSIMHAVLAYREHGRKERGIERPNIIKPETAHAAFDKAAHLFGL
                     ELRRAPVDPVTTKVRPEVVADLIDESTVAVIGSACNYGYGTVDPIAQLGALAQERGIG
                     LHVDGCLGGFILPWGEELGYPVPPFDFRVPGVTSISADTHKYAYGLKGTSVLLFRDRA
                     LRNSQYFFLTDWTGGKYCSPGMEGSRSGGLLAATWAGMLKLGRGGYLRYAQQIFEASA
                     AMQAAVTSHPELRLLGEDPTFLFSVTSNEFDVYHVNDFMRGRGWRFNGQQYPNALHMA
                     VTRPQTQAGVTEAFATDLADAVAYAKEQGDEPPKSGAIYGGIPGGMTPDADEFIRAVM
                     TDMMDDHQALPA"
     gene            2755..>2940
                     /locus_tag="VFP02_02690"
     CDS             2755..>2940
                     /locus_tag="VFP02_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET9541960.1"
                     /translation="MSGSALGIDVGSTNVKVARVDETGRLEASTQRPLVTRRDGDVVE
                     QDADALWSAVTAAVAEVA"
BASE COUNT          483 a         1051 c         1026 g          380 t
ORIGIN      
        1 cgtgcccggt gcccgggtag atcgtgaagc tgacgtcctt gcccatggcc tgcagccggg
       61 actccaggtc ccgcacggcc tcgggcggga agaagtcgtc gttctcggcg aagtggccct
      121 cgaccttggc cgtgagccca gaccagtccg gctcgccgtc acccaaggga gccccgtaga
      181 aggtcacggc agccgccacc cggtcaccct ccagcgccga gatcatcagg gtgagcaggc
      241 cgcccatgca gaagccgatg accccgaccc ggacacccgt gacggcgtcg ctggccagga
      301 ggtgggcgat cgccccggcc atgtccttgg cggcccgttc cggcggcagg ctgttcatca
      361 gctcgccggc cttgtccatc tcggtgtgct cggcgaagtc accgtggaag aggtccgggg
      421 ccagagcggt gaacccctca ccggccaagc ggtcgcacac cgctttgatc tgggggacga
      481 ggccccacca ctcctggatc acgaggaggc ccggcccgga ccccccgtcg ggtgtggaca
      541 ggtagccgtc ggcctcggtg ccattgaccg ggaacgaaac catgctggcg tcgaccatcg
      601 gttgtgtacc tccagtgcgg ggacgacgcg aacgtagtca gttcgcctca gggccgcgga
      661 cgggatcagg ccttgcgccc accggagaac gccagcccga ccccgagccc gatcatggtg
      721 atcccgccgg ccccacccag tcgctcgagc cggcgcggcg aggacgagag ccagacccgg
      781 gccgaccctg cggcaaggcc ccaggccccg tccgagacca gggccacccc gacggagacg
      841 agggccagca cggccatctg gaggccggcc ggagcgccac cgggctcgac gaactgcggg
      901 aggatggcgg cgaagaacac ggcggccttc gggttggcca gcccgacgac gaagccctcc
      961 cgcagcaggt gcttcgtcgg ccggaccgtg ccggcggcgt cgagcacctc cgagagggcc
     1021 cgccgatggc ggaccgcctg gatccccaag tacatgaggt agccggcccc gaccagcttg
     1081 atggcgttga acacggtggc cgaccggctg atgaccgccc ccagcccgac ggcgacgagc
     1141 agggcctgga cgtagacgcc ggccgcgttg ccgatcacgg tggcgagggc cgctcggcgg
     1201 ccgagagcca cgccgcggct gatgacgaac aggacgctgg gcccgggcac gatcaccagc
     1261 aggaacgagg ccagggcgaa cgtggcgaac gagtgcaacg acggcatcgc cggaagctac
     1321 cggccccggc cccgcgaccc gtccccgatt cggccagact caggccatga gcacgcatcc
     1381 ctacgccgag cggttcgccg tcaaccgtcg cctgcccgag tccggccgcc aggaaggcga
     1441 gttgctcgac gagctgcagc agatggccac cgaggaggac gcgtactggg agaccggcaa
     1501 gtgctccggg acgatgtact gcggcgacca tgagcactac gccttcatgg gggaggcctt
     1561 ccagctcttc gcccacgcca acatcctcca gcgggacatg ttccccagcg ccaccaagct
     1621 ggaaggcgag atcatcgcca tgaccctgga cctcatgcac ggtgccgagg ccgagggcgg
     1681 tgacgccgtg ggcatggtca ccacgggcgg gaccggcagc atcatgcacg ccgtcctcgc
     1741 ctaccgggag cacggtcgca aggagcgggg gatcgagcgg cccaacatca tcaagccgga
     1801 gacggcccac gccgccttcg acaaggcggc ccacctcttc ggcctggagc tgcgccgggc
     1861 gccggtggac cccgtgacca ccaaggtcag gcccgaggtc gtggccgacc tcatcgacga
     1921 gtcgaccgtg gccgtcatcg gttcggcctg caactacggc tacgggacgg tcgatccgat
     1981 cgcccagctc ggggccctgg cccaggagcg ggggatcgga ctccacgtgg acggctgcct
     2041 cggtggcttc atcctgccgt ggggcgagga gttggggtat cccgtcccgc cgttcgactt
     2101 ccgcgtgccg ggagtgacgt cgatctcggc cgacacccac aagtacgcct acgggctcaa
     2161 gggcacctcc gtgctgctct tccgggaccg ggccctgcgc aacagccagt acttcttcct
     2221 gaccgactgg acggggggca agtactgctc gccggggatg gaggggtccc gctccggcgg
     2281 cctgctggcc gccacctggg cggggatgct caagctgggc cggggcgggt acctgcgtta
     2341 cgcccagcag atcttcgagg cgtcggccgc catgcaggcc gcggtgacca gccaccccga
     2401 gctgcgcctg ctgggcgagg accccacgtt cctcttctcg gtcacctcca acgagttcga
     2461 cgtgtaccac gtcaacgact tcatgcgggg ccggggctgg cgcttcaacg gccagcagta
     2521 ccccaacgcc ttgcacatgg ccgtgacccg gccccagacc caggccgggg tgaccgaggc
     2581 gttcgccacc gacctggccg atgctgtcgc ctacgccaag gagcagggcg acgagccccc
     2641 caagagcggg gcgatctacg gcgggatccc ggggggcatg acgcccgacg ccgacgagtt
     2701 catccgagcc gtgatgaccg acatgatgga cgaccatcag gccctgccgg cctgatgagc
     2761 gggtcggccc tcggcatcga cgtcgggagc acgaacgtca aggtggctcg ggtcgacgag
     2821 accggccggc tggaggcctc gacccagcgg ccgctggtga cccggcgcga cggtgacgtg
     2881 gtcgagcagg acgccgacgc cctctggtcc gccgtcactg cggccgtggc cgaggtggcc
//
LOCUS       DASTFV010000144         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_509184, whole genome shotgun sequence.
ACCESSION   DASTFV010000144 DASTFV010000000
VERSION     DASTFV010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509184"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VFP02_02695"
     CDS             <1..686
                     /locus_tag="VFP02_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SecD/SecF family protein translocase subunit"
                     /protein_id="HET9541961.1"
                     /translation="RDQIQISGDFDESSAKDLALVLEYGALPVELVPQQTQTVSATLG
                     EDSLRAGLVAGIIGLGLTVIFMVGYYRLLGVVAVVSLTLEFALVWVLISYLGESRGLA
                     LTLAGVTGLIVSIGISVDSNVVFYEHLKEDVRNGRTFRSTVDRSFGSSFTTIIKADLA
                     SLIGAFLLYFLSVGPVRGFALLLGISTVVDLIATWFFIRPAVLILARSPRYRNNYAAL
                     GMPNVEAGS"
     gene            683..1819
                     /gene="secF"
                     /locus_tag="VFP02_02700"
     CDS             683..1819
                     /gene="secF"
                     /locus_tag="VFP02_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HET9541962.1"
                     /translation="MTDDMATTEDSAVEPERSGLRANLRALYQCTNDFDFTRLFKRGL
                     VIAVLVSVISIISLFTRGLNLGIDFKGGSVWEVPVTDVSVEDARDVMEGVGLGDAKIQ
                     LAVNADGTKILRVQADVTEVDASREVTGDLADLAEVPVSDIAVNTVGPSWGDEITRQA
                     QRALVYFFIAITLYISFRLEWKMAVGALVSMAHDLLLSVGVYSILRIEVTPATLISFL
                     TILGYSLYDTIVVFDRALENSSRTVAKTPYRVIMNRTLNQTLMRSVNTTVATILPIIS
                     MLIFGALLLGAKTLQEFGLALLVGLTSGAYSSLFVAAPIVSWLKEREPKFAYTGEAPV
                     AMAAAVDGESTGRPARRGAARRPATPAAPVGAIPPRPRKTKKRK"
     gene            1860..>2315
                     /locus_tag="VFP02_02705"
     CDS             1860..>2315
                     /locus_tag="VFP02_02705"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET9541963.1"
                     /translation="MTDASWLTGLVRDIPDFPEPGITFRDITPLLGDVDAFRFVVDAL
                     ADEFAGETIDRVLGIEARGFLLAAPVAYRLGAALVPVRKSGKLPHDTHTESYQLEYGS
                     DALELHVDALGAGDTCLVIDDVLATGGTAAAVGRLIERLGGRLAGYGFLI"
BASE COUNT          330 a          841 c          741 g          403 t
ORIGIN      
        1 agcgggacca gatccagatc agcggtgact tcgacgagtc ctcggccaag gaccttgccc
       61 tggtgctcga gtacggggcc ctgccggtcg agctcgtgcc ccagcagacc cagacggtgt
      121 cggcgacgct gggtgaggac tccctgcggg ccgggctcgt cgccggcatc atcggcctgg
      181 gcctgaccgt catcttcatg gtcggctact accggctgct gggcgtggtg gccgtggtca
      241 gcctcaccct ggagttcgcc ctcgtgtggg tcctcatctc gtacctcggc gaatcccggg
      301 gcctggccct gaccctggcc ggcgtcaccg gcctgatcgt gtccatcggc atctccgtgg
      361 actccaatgt cgtcttctac gagcacctga aagaggacgt ccgcaacggg cgcaccttcc
      421 gcagcaccgt cgaccgctcg ttcgggtcga gcttcaccac gatcatcaag gccgacctgg
      481 cctcgctgat cggggccttc ctcctgtact tcctctcggt cgggccggtc cggggcttcg
      541 ccctcctgct cggcatctcg acggtcgtgg acctgatcgc cacctggttc ttcatccggc
      601 ccgcggtcct gatcctggct cgcagcccga ggtatcggaa caactacgcc gcgcttggca
      661 tgcccaacgt ggaggcggga tcatgacgga cgacatggcc accaccgagg actccgccgt
      721 ggagccggaa cggtccggcc tgcgggcgaa cctccgggcg ctctaccagt gcaccaacga
      781 cttcgacttc acccgcctct tcaaacgggg cctcgtcatc gccgtgctgg tgtcggtgat
      841 ctccatcatc agcctcttca cccgcggcct gaacctgggc atcgacttca agggcgggag
      901 tgtctgggag gtcccggtca ccgacgtgtc ggtcgaggac gcccgcgacg tgatggaggg
      961 cgtgggcctc ggtgacgcca agatccagct ggcggtgaac gccgacggga ccaagatcct
     1021 gcgggtccag gccgatgtca ccgaagtgga cgccagccgt gaggtcacgg gcgacctggc
     1081 cgacctggcg gaggtccccg tctccgacat cgccgtgaac acggtcggac catcctgggg
     1141 cgacgagatc acccggcagg ctcagcgcgc cctcgtctac ttcttcatcg ccatcacgct
     1201 gtacatcagc ttccggctgg agtggaagat ggccgtgggc gccctggtgt cgatggccca
     1261 cgaccttctg ctgagcgtgg gcgtgtactc catcctccgg atcgaggtca cgccggcgac
     1321 cctcatctcg ttcctgacca tcctgggcta ctcgctctac gacacgatcg tggtgttcga
     1381 ccgcgccttg gagaacagct cccgcacggt ggccaagacg ccgtaccggg tgatcatgaa
     1441 ccggacgttg aaccagaccc tgatgcggtc ggtcaacacg acggtcgcca cgatcctgcc
     1501 catcatctcg atgctgatct tcggcgccct cctgctcggg gcgaagaccc tgcaggagtt
     1561 cggtctcgcc ctcctggtcg gcctcacctc gggggcctac tcgtccctct tcgtcgccgc
     1621 cccgatcgtc tcgtggctga aggagcggga gcccaagttc gcctacaccg gcgaggcgcc
     1681 ggtggccatg gccgccgcgg tcgacggcga gtcgacgggc cgtcccgccc ggcgcggcgc
     1741 cgctcgtcgt ccggctaccc cggccgctcc ggtcggcgcc atcccgcccc gcccccgcaa
     1801 gaccaagaag cgcaaatagc tccccgagaa cggcgggtcg ccgacggtag attcgtctga
     1861 tgaccgacgc atcgtggctc accgggctcg tgcgcgacat ccccgacttc cccgagccgg
     1921 gcatcacctt ccgcgacatc accccgctgc tcggcgacgt cgacgccttt cgcttcgtgg
     1981 tcgacgccct ggccgacgag ttcgccggtg agaccatcga ccgggtcctg ggcatcgagg
     2041 cccgcggctt cctgctcgcc gccccggtcg cctaccgcct cggcgccgcc ctggtgccgg
     2101 tgcgcaagtc ggggaagctg ccccacgaca cccacaccga gagctaccag ttggagtacg
     2161 gcagcgatgc cctggagctg cacgtcgacg ccctgggggc gggggacacc tgcctggtca
     2221 tcgacgacgt gttggccacc ggcggcaccg cggcggcggt cggccgactg atcgagcgcc
     2281 tgggcggccg tctcgccggc tatggcttct tgatc
//
LOCUS       DASTFV010000145         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_511808, whole genome shotgun sequence.
ACCESSION   DASTFV010000145 DASTFV010000000
VERSION     DASTFV010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511808"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1229
                     /locus_tag="VFP02_02710"
     CDS             84..1229
                     /locus_tag="VFP02_02710"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9541964.1"
                     /translation="MSTERTVDQDRLADLFARAQRDVDAGTLPACQVAVALDGELVAE
                     ATFGAPEGSRFHGYSSGKVVVAGAVWVLLGEGKLRLEDRVAEHIPEFGPNGKDTITVE
                     QVLTHTSGFPRAPFAPLQWEDRDARLGRFASWRTNWEPGTRFEYHATSAHWVLAELIE
                     RAAGQDWRQFVRDRLLEPVGLDHLRFGVPPEDQGDIVDVVTVGEAPTSAELEALTGVA
                     GLDLAAFQGEVTDEVLISFNRPDVRAVGVPGGGVITTAGDLALWYQALLHDPRGLWDP
                     TVLADGTGNVRCTFPDPMTSAPANRTIGVIVRGDDDQIALRGMGRTVSARAFGHNGAG
                     GQVAWGDPGSGLSFAYLTNGLDRHPIASGRRGAAMSNRAGALMTPGG"
     gene            1268..>2478
                     /locus_tag="VFP02_02715"
     CDS             1268..>2478
                     /locus_tag="VFP02_02715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HET9541965.1"
                     /translation="MERLSGMDASFLYAETPTGHTHVTGVIVIDAAKMTGGYQFDRVV
                     DMIEQRLHRLKPFRRRLVEVPLSFDHPVWIEDPDFDITNHMHRVTWPAPGRTQELAAL
                     VGDLMSRPLDRQRPLWEMWVAEGGKEGRVALISKIHHSAIDGVTGADLLSQLFDLEPE
                     APPVDPPDEPWEPEPIPTETQLLVDAIAARIKDPWRAARAARRTLNSVAGVVSTVRNK
                     GEERTPAMPFTGPRTLFTGSLSADRAVAFGKAPLEDLKVVKNVYGCKLNDVVLATCSM
                     ALRKYHESHGEIPDKPLLIMCPVSTHSPDGEGTNQVSSMAVRLPVHMADPVDQLLEIF
                     EDTKVAKEMQSAMGAEMLSDITQFAPPALFNQAMRLYSRSGLADRHTPVQNGVVSNIP
                     GPPIPMWMVGAP"
BASE COUNT          376 a          945 c          821 g          336 t
ORIGIN      
        1 gccaccgtgg cgaacgagcc gatgaatccc gtcccaccgg tcaccaggat gcgcaccgcg
       61 gcggacggta gcgtccgcgg ctgatgagca ccgagaggac cgtggaccag gaccgactcg
      121 ccgatctctt cgcccgggct cagcgggacg tcgatgccgg caccctgccg gcctgtcagg
      181 tggccgtcgc cctcgacggc gagctcgtgg cggaggccac cttcggcgcc ccggagggca
      241 gtcgcttcca cggctactcg tcgggcaagg tcgtcgtcgc cggcgccgtc tgggtcctcc
      301 tgggcgaggg gaagctgcgc ctggaggacc gggtcgccga gcacatcccc gagttcgggc
      361 ccaatggcaa ggacaccatc accgtcgaac aggtcctgac ccacaccagc ggcttccccc
      421 gggccccgtt cgcaccgttg cagtgggagg accgggacgc ccgccttggc cggttcgcgt
      481 cctggcggac caactgggag cccggcaccc gcttcgagta ccacgcgaca tcggcccact
      541 gggtgctggc cgagctcatc gagcgagccg ctggccagga ctggcgtcag ttcgtgcggg
      601 accgactgct cgagcccgtg gggctggacc acctgcgctt cggcgtgccg cccgaggacc
      661 aaggcgacat cgtcgacgtg gtcaccgtcg gtgaagcccc gacctcggcc gagctggagg
      721 ccctcaccgg cgtcgccggc ctcgatcttg ccgccttcca gggcgaggtg accgacgagg
      781 tcctgatcag cttcaaccgc cccgacgttc gggccgtggg cgtgcccggc ggcggggtca
      841 tcaccacggc cggcgacctg gcgctctggt atcaggccct cctccatgac ccgaggggac
      901 tgtgggaccc caccgtgctg gccgacggca ccggcaacgt ccgctgcacc ttccccgacc
      961 cgatgacctc ggcaccggcc aatcgcacga tcggcgtgat cgtgcggggt gacgacgacc
     1021 agatcgccct gcggggcatg ggccgcaccg tgtcggcccg cgccttcggc cacaacggcg
     1081 cgggcggcca ggtggcctgg ggcgaccccg ggtcgggatt gtccttcgcc tacctcacca
     1141 acggcctcga ccggcacccg atcgcgtccg gccgccgggg tgccgccatg tccaaccgcg
     1201 ccggggccct catgacgccc ggggggtagc gttcgcccgc ggacggcccg cggcgagggg
     1261 gaacaggatg gaacggctct ccgggatgga cgcctcgttc ctctacgccg agacgccgac
     1321 cgggcacacg cacgtgaccg gggtcatcgt catcgatgcc gccaagatga ccggcgggta
     1381 ccagttcgac cgggtcgtcg acatgatcga gcagcgccta caccggctga agcccttccg
     1441 ccgccggctg gtcgaggtgc cgctgtcctt cgaccacccc gtgtggatcg aggatccgga
     1501 cttcgacatc accaaccaca tgcaccgcgt cacgtggccc gctccgggcc gcacccagga
     1561 gctggccgcc ctggtcggcg acctgatgtc ccggcccctc gaccgccaac gccccctctg
     1621 ggagatgtgg gtggccgaag gcggcaaaga gggccgggtc gccctgatct ccaagatcca
     1681 ccactccgcc atcgacggcg tcaccggggc cgacctcctg agccagctct tcgacctgga
     1741 gcccgaggcc cccccggtcg atcctcccga cgagccgtgg gagcccgagc ccatcccgac
     1801 cgagacccag ctgctcgtcg atgccatcgc cgcccgcatc aaggacccgt ggcgagccgc
     1861 ccgggccgcc cgccgcacgc tcaactcggt cgccggcgtc gtgagcaccg tcaggaacaa
     1921 gggcgaggag cgcaccccgg ccatgccctt caccggcccc cgcaccctct tcaccgggtc
     1981 gctgagcgcg gaccgggcgg tggccttcgg caaggccccg ctggaggacc tcaaggtggt
     2041 gaagaacgtc tacggctgca agctcaacga cgtcgtcctg gccacctgct ccatggccct
     2101 gcgcaagtac cacgagagcc acggcgagat ccccgacaag ccgctgctca tcatgtgccc
     2161 ggtgtccacc cacagccccg acggcgaggg gaccaaccag gtctcctcca tggccgtgcg
     2221 cctgcccgtg cacatggccg atcccgtcga ccagctgctc gagatcttcg aggacaccaa
     2281 ggtggccaag gagatgcaga gcgccatggg cgcggagatg ctcagcgaca tcacccagtt
     2341 cgcgccgccg gccctgttca accaggccat gcgcctctac agccggagcg ggctggccga
     2401 tcgccacacc ccggtccaga acggcgtcgt ctccaacatc cccgggccgc cgatcccgat
     2461 gtggatggtc ggggcccc
//
LOCUS       DASTFV010000146         2668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_512096, whole genome shotgun sequence.
ACCESSION   DASTFV010000146 DASTFV010000000
VERSION     DASTFV010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512096"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VFP02_02720"
     CDS             <1..825
                     /locus_tag="VFP02_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541966.1"
                     /translation="EDAEAAEAIEDRHAAVLSAQDDHERVRKLAVYVATFSAIGAVPL
                     AMLEGRSMAMPFVLFAAVAAVTSIIFWGRVRMAQTAEADALEAAGMPSYLGFHLDRVN
                     SLLATDKARRVLLEASEVHRSARGEWEQLVGNGISTEWAMEHKDEIVAAARLRQDVTS
                     SSAIGMTGGGDLIARYAQALNTRLAAVRGVGARHQTLPLILDDTLTGIDAALKPPLLE
                     LLSKATVSQQVLFLTNDEDVISWARLEAIAGHLALIEPAAGLSVTPEPDVVEVDLR"
     gene            847..1353
                     /locus_tag="VFP02_02725"
     CDS             847..1353
                     /locus_tag="VFP02_02725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9541967.1"
                     /translation="MKIRRATVDDAEAIRAIYNAEVTGSTVTFDLVPRTAQEQVAWLV
                     ERSGAYAVLVAEDDDGTVLGFASLSPFRERPAYSTTVEDSVYVGRDRRGGGVGGALLE
                     ELVATATAHGFHTVIARIVGGHDASIALHQKAGFDVVGTEREVGRKMGRWLDVVVMQR
                     LLGDGGAV"
     gene            1385..2002
                     /locus_tag="VFP02_02730"
     CDS             1385..2002
                     /locus_tag="VFP02_02730"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006822499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET9541968.1"
                     /translation="MSTYTLPDLPYDHAALEPHLDARIMELHHGKHHAAYVDQSNKAL
                     DQLAEARSSGDFALITKLSKDLAFNLSGHVLHTVFWTCMSPDGGGAPEGELAAAIEDA
                     FGSFDDLKAQLTEATKSVQGSGWGALAWEPLAGRLIVEQVYDHQSNIGQGSDPILVID
                     AWEHAFYLQYLNDKAAWVDAFWEVADWQSAAARFDAVQTTGTATR"
     gene            1999..2352
                     /locus_tag="VFP02_02735"
     CDS             1999..2352
                     /locus_tag="VFP02_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9541969.1"
                     /translation="MSSSLGDVHDGEDRLMVETAEGDPPTIALIGEVDPHTAPLLADA
                     LDAVIDKGATVVRIDGRRLDFIDSSGLRVLVDAHRRLGSDQNVLVLAHVSPTLRRLLE
                     VTGLDEHVTVESTSS"
     gene            complement(2363..>2668)
                     /locus_tag="VFP02_02740"
     CDS             complement(2363..>2668)
                     /locus_tag="VFP02_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9541970.1"
                     /translation="EVTPTASADLLLVATELCTNAIEATPPDVPVEVRVSNDGTALRL
                     TVANHRPVDSAEPEPAVLQRGSLQERGRGLAIVRSLVDTFVMTSDDDRTVVRAMRLL"
BASE COUNT          404 a          979 c          911 g          374 t
ORIGIN      
        1 gaggacgccg aggcggccga agcgatcgag gaccgccacg ctgccgtgtt gtccgctcag
       61 gacgaccacg agcgcgtccg gaagctggcc gtctacgtcg ccaccttcag cgccatcggc
      121 gccgtgcccc tggcgatgct cgagggccgc agcatggcca tgcccttcgt gctcttcgcc
      181 gcggtcgccg ccgtgacctc gatcatcttc tggggtcggg tgcgcatggc ccagaccgca
      241 gaggccgatg ccctcgaggc ggccgggatg ccgtcgtacc tcgggttcca cctcgaccgg
      301 gtcaattccc tgctggccac cgacaaggcc cgccgcgtcc tgctggaggc ctccgaggtc
      361 caccggtcgg cgcggggcga gtgggagcag ctggtcggga acggcatctc gaccgagtgg
      421 gccatggagc acaaggacga gatcgtggcc gccgcccgcc tgcgccagga cgtgacctcc
      481 tccagcgcca tcggcatgac cggcgggggc gacctgatcg cccgctacgc ccaggccctg
      541 aacacgcggc tggccgcggt ccgaggcgtc ggggctcgcc accagaccct gccgctgatc
      601 ctcgacgaca ccctcacggg catcgatgcc gccctgaagc ctccgctgct cgagctcctg
      661 agcaaggcca ccgtgagcca gcaggtcctg ttcctgacca acgacgagga cgtcatctcc
      721 tgggcccggc tggaggccat cgccggccac ctggccctca tcgagccggc ggccgggctg
      781 tcggtcaccc cggagcccga cgtcgtcgag gtcgacctgc gctgacgggg cagactgccc
      841 cccgccatga agatcagacg ggccacggtg gacgacgccg aggcgatacg ggccatctac
      901 aacgccgagg tcaccggctc cacggtgacc ttcgacctgg tgccccgcac ggcccaggag
      961 caggtcgcgt ggctggtcga gcgttcgggc gcctacgccg tgctggtggc cgaagacgac
     1021 gacgggacgg tgttgggctt cgcctcgctg tccccgttcc gggagcgacc ggcctacagc
     1081 acgaccgtgg aggactcggt ctacgtcggg cgtgaccgcc gcggcggcgg ggtcggcggg
     1141 gcgctgctcg aggagctggt ggccacggcc accgcccacg gcttccacac cgtcatcgcc
     1201 cgcatcgtcg gcggccacga cgcgtcgatc gcgctccacc agaaggccgg cttcgacgtg
     1261 gtgggcactg aacgggaggt cgggcgcaag atggggcgct ggctggacgt cgtggtgatg
     1321 caacgccttc tcggcgacgg cggggccgtc tgatttctcc ccgatcgggg tagacgtgtg
     1381 ccccatgagc acgtacaccc tccccgacct gccgtacgac cacgccgccc tcgagcccca
     1441 cctcgacgcc cggatcatgg agctccacca cggcaagcac cacgcggcct acgtggacca
     1501 gtccaacaag gccctcgacc agctggccga ggcccgcagc agcggcgact tcgccctcat
     1561 caccaagctg agcaaggacc tcgccttcaa cctctcgggc cacgtcctgc acaccgtctt
     1621 ctggacctgc atgagccccg acggcggggg tgcccccgag ggcgagctgg ccgcggccat
     1681 cgaggacgcc ttcggcagct tcgacgacct caaggctcag ctcaccgagg ccaccaagag
     1741 cgtccaggga tcgggctggg gagccctcgc ctgggagccg ctggccggcc gtctcatcgt
     1801 cgagcaggtg tacgaccacc agagcaacat cggccaggga tcggacccca tcctggtcat
     1861 cgacgcctgg gagcacgcct tctacctcca gtacctcaac gacaaggcgg cgtgggtcga
     1921 cgccttctgg gaagtggcgg actggcagag cgcggcggcc cgcttcgacg ccgtccagac
     1981 caccgggacg gccacccggt gagcagtagt ctcggcgacg tgcacgacgg cgaggaccgg
     2041 ctcatggtcg agaccgccga gggcgacccg ccgacgatcg ccctcatcgg cgaggtcgat
     2101 ccccacactg ccccactcct ggccgacgcc ttggacgcgg tcatcgacaa aggggccacg
     2161 gtcgtgcgca tcgacggccg ccgtctcgac ttcatcgact cgtccggcct ccgggtgctg
     2221 gtcgacgccc accgccggct gggcagcgac cagaacgtgc tggtcctggc ccacgtgtca
     2281 cccacgctgc ggcgcctcct cgaggtcacc ggcttggacg agcacgtgac cgtcgaatcg
     2341 acgtcgtcct gagcgcagcc gctcagagca ggcgcatggc ccggaccacg gttcgatcgt
     2401 cgtcggaggt catcacgaac gtgtccacca gcgaacgcac gatggccagg ccacggcccc
     2461 gttcctgcag tgagccccgc tgcagcacgg cgggctcggg ctcggccgag tccaccggtc
     2521 ggtggttggc cacggtcagg cgcagggcag tgccgtcgtt gctcacccgg acctccacgg
     2581 ggacgtcggg cggggtggcc tcgatggcgt tcgtgcacag ctcggtggcc accaggagca
     2641 ggtcggcgga tgccgtgggg gtgacctc
//
LOCUS       DASTFV010000147         5134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_512156, whole genome shotgun sequence.
ACCESSION   DASTFV010000147 DASTFV010000000
VERSION     DASTFV010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512156"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VFP02_02745"
     CDS             <1..967
                     /locus_tag="VFP02_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541971.1"
                     /translation="SLPLEDADEAARFVLQALPRLPAAPTLPTAHPVEGMLAQAAWGL
                     RGVTVADDGSLAVRPLELDGHHPYAEDAGVGGPPFAALQAFLRAVKGRTGPVKLQLTG
                     PVTLGRALADAGAPADVAFAVAGSAVRERVETLLAAARHAVPSAPLVVFLDEPGLTGN
                     EHPRFPLANEEVVRLLSESLASIELDAISGVHCCGPTDWSLVTQAGPRVLSLPVTAGV
                     VTHAGTLGRFLDRDGWIAWGAVPTDEPVGETGDGLWRRLSDLWCGLVREGCDAGRLRR
                     QALITPACGLAAHAERQADHRLRLATELGRRVLGQAIGVRLQVGA"
     gene            1011..3077
                     /gene="ligA"
                     /locus_tag="VFP02_02750"
     CDS             1011..3077
                     /gene="ligA"
                     /locus_tag="VFP02_02750"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET9541972.1"
                     /translation="MATDPTADEITAAERVQELAEQIRFHNERYHTLDDPLISDADFD
                     ALVRELKALEAEFPDLATPDSPTQDVGGAPSTTFAPVEHAVRMMSLDNAFELEELVAW
                     GARLQRRLGQAADAAVDYVCELKIDGLAVSIRYEEGRYVQAATRGNGRVGEDITPNVA
                     TLAQVPEKLTGDVPDVLEIRGEIYMPIPAWNALNEEQERQGLARYVNPRNTAAGSLRQ
                     KDAAITATRNLALWTYQLGQVQGGPTFTTHHETLDYIRSLGLPVNPKIERLSGLEEIH
                     QYCVGWLEHRHDLDYEIDGVVVKVDDLALRDELGSTAKAPRWAIAFKFPPEERTTLLK
                     DIMVSIGRTGKATPFALLEPVFVGGSTVGLSTLHNQDQVAAKDVRPGDTVTVRKAGDV
                     IPEVVGPVLGLRRKGLRKWKFPTVCPVCGGPLVRLEGESDTFCTNLDCPAQIAGRIEH
                     FASRGAMDIEGFGEQRAQLFTHLDPETGTRLITDVGDIYSIDWDVILGMEGFGEIAVR
                     NLQEAIESSRSRPLANLLVGLNIRHLGGTMAELVAMGMGSLDAIMDASEEELVALDGV
                     GPIIARSVHEFFQSDANRAVVEKLRAAGLNFDGPERPDVPQTLDGISIVVTGTLDGFT
                     REEIEEAIKSRGGKAPGSVSKKTTAVVLGRDPGQAKATKAEELGIPRIDEAAFRHLLD
                     TGELPT"
     gene            complement(3070..3495)
                     /locus_tag="VFP02_02755"
     CDS             complement(3070..3495)
                     /locus_tag="VFP02_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541973.1"
                     /translation="MSDSLRRVLVVLAVVACLAGIVFAFTLVDTDDGTNDIAVTEAGP
                     VEQLIPPRGSEILRQEAVGVDLRPGWTGVLVINGVEIPEDQIDDANLASLGQLLYTAG
                     DGKAVERFEAGENCITAVVWRVEESRADSRDVDWCFNVT"
     gene            3592..>5134
                     /locus_tag="VFP02_02760"
     CDS             3592..>5134
                     /locus_tag="VFP02_02760"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9541974.1"
                     /translation="MAMTRMNDHVGRVLAGRYRLLAPIGAGGSAQVFLAEDTRLKRRV
                     AVKLLHESYARDEAFIRRFRSEAQTAAGLNHPNLLAVFDWGEDEVPFLVTEFIGGGSL
                     RAMLDQGQRLSISQATLIGLEAAKGLDHAHRQGLVHRDLKPANLLFGEDERLRIADFG
                     LARALADAAVTEPSGAVLGTARYASPEQAQGRPLDGSSDIYSLALVLIEAVTGQVPFS
                     VDTTIGTLMARVAEPVPVSNRLAVLQPILRAAGAVEPEDRPDAARLATMLIKASEELD
                     PPERLPLAGAIVLADTAVEIDRDPTMLPEPSPGGTVFPDEAPVPPAEIAGPGRLGRRR
                     QRRAARKAARGDRRTGRRVLIGVLTVVLLAAVAAGGYAAYQVLRPEHEVPDLAEQTVG
                     NLDDLVGDLGFDVQLVGVRRNGAPAGTVVEQDPAEGEKLREGQTLTVTVSRGPEFLDV
                     PANLTNITQAQSEAAIEAAGFRVGHVSEKYNELVVAGIVQSVITPFDEVPRNSAIDLV
                     VSLGPR"
BASE COUNT          745 a         1938 c         1752 g          699 t
ORIGIN      
        1 cagcctgccg ctcgaggatg ccgacgaggc agcccgattc gtcctccagg ccctgcctcg
       61 cctccccgcc gcgcccaccc tgccgaccgc ccaccccgtc gaggggatgc tggctcaagc
      121 ggcctgggga ctgcgcggcg tgaccgtggc cgacgacggc agcctggccg tgcggccgct
      181 cgagctcgac ggccaccatc cgtacgccga ggacgcgggc gtcggcgggc caccgttcgc
      241 cgcactgcag gccttcctcc gcgccgtcaa gggccgcacg ggcccggtga agctccagct
      301 cacaggcccg gtgaccctcg ggcgagcgct ggccgatgcc ggggctccgg cggacgtggc
      361 cttcgccgtg gccggctcgg ccgtgcggga gcgggtggag acgctcctgg ccgccgcccg
      421 ccacgccgtg ccgtcggccc cgctggtcgt cttcctcgac gagcccggcc tcaccggcaa
      481 cgagcacccg cgcttcccgc tggccaacga ggaggtcgtc cggctcctca gcgagtcgct
      541 ggcttcgatc gagctggacg ccatcagcgg ggtgcactgt tgcgggccga ccgactggtc
      601 gctggtcacc caggccgggc cccgggtcct gtccctgccc gtgacggccg gcgtcgtcac
      661 ccatgccggc accctcggcc ggttcctgga ccgggacggc tggatcgcct ggggggccgt
      721 gcccaccgac gaacccgtgg gggagacggg cgacggcctc tggcggcggc tctccgacct
      781 ctggtgcggc ctggtgcgcg agggctgcga cgccggtcgc ctgcgccgtc aggccctgat
      841 cacccccgcc tgcggcctgg ctgcccacgc cgagcgccaa gccgaccacc gccttcgcct
      901 ggccaccgag ctgggtcggc gagtgctggg ccaggccatc ggcgtgcgcc tccaggtcgg
      961 cgcctgacga gccggccgtt gccgtcactg gtcgccggta ggttctgacc gtggcgaccg
     1021 accccaccgc cgacgagatc accgccgccg agcgggtcca ggagctggcg gagcagatcc
     1081 ggttccacaa cgagcgctac cacacgctcg acgacccgct gatcagcgac gccgacttcg
     1141 acgccctcgt ccgtgagctg aaggccctgg aggccgagtt cccggacctg gccacgcccg
     1201 actcgccgac gcaggatgtc ggcggcgccc cgtccaccac cttcgccccg gtcgagcacg
     1261 ccgttcggat gatgtcgctg gacaacgcct tcgagctgga ggagctcgtg gcctggggcg
     1321 cccgcctcca gcgtcggctg ggccaggccg cggatgcggc ggtggactac gtctgcgagc
     1381 tgaagatcga cggcttggcc gtgtccatcc gctacgaaga gggccgctac gtccaggccg
     1441 ccacccgggg caacggtcgg gtcggcgagg acatcacccc caacgtggcc accctggccc
     1501 aggtccccga gaagctgacg ggcgacgtcc ccgacgtgct ggagatccgg ggcgagatct
     1561 acatgcccat cccggcgtgg aacgccctca acgaggaaca ggagcgccag ggcctggccc
     1621 gttacgtgaa cccccgcaac actgcggcgg gctcgctgcg ccagaaggac gcggcgatca
     1681 cggccacgcg caacctggcg ctgtggacct accagctcgg ccaggtccaa ggcgggccga
     1741 ccttcaccac ccatcacgag accctggact acatccggtc cctcggcctg cccgtcaacc
     1801 cgaagatcga gcgcctgtcc gggctggagg agatccacca gtactgcgtg ggctggctgg
     1861 agcaccgcca cgacctcgac tacgagatcg acggcgtcgt ggtgaaggtg gacgacctgg
     1921 ccctgcgcga cgagctgggc tcgacggcca aggcaccccg ctgggccatc gccttcaagt
     1981 tcccgcccga ggaacggacg accctgctca aggacatcat ggtgtccatc gggcggaccg
     2041 ggaaggcgac gccgttcgcc ctgctcgagc ccgtcttcgt cgggggctcg accgtcggcc
     2101 tgtccaccct ccacaaccag gaccaggtgg cggccaagga cgtccggccg ggggacaccg
     2161 tcaccgtgcg caaggcgggc gacgtgatcc ccgaggtcgt cggccccgtg ctcgggctgc
     2221 gcaggaaggg cctgcgcaag tggaagttcc cgacggtctg cccggtctgc ggcggtccgc
     2281 tggtccggct ggagggcgag agcgacacct tctgcacgaa cctcgactgt cccgcccaga
     2341 tcgccgggcg catcgagcac ttcgcctccc gaggcgccat ggacatcgag ggcttcggtg
     2401 agcaacgggc ccagctgttc acccacctcg atcccgagac gggcacccgg ctcatcaccg
     2461 acgtcggcga catctacagc atcgactggg acgtcatcct gggcatggaa ggcttcggcg
     2521 agatcgccgt gcgcaacctc caggaggcca tcgagtcatc gcggtcacgg ccgctggcca
     2581 acctgctggt gggcctcaac atccggcacc tgggtgggac catggccgag ctcgtggcga
     2641 tgggcatggg cagcctcgac gccatcatgg acgcctccga ggaggagctg gtcgccctcg
     2701 acggcgtcgg cccgatcatc gcccgcagcg tccacgagtt cttccagtcc gacgccaacc
     2761 gggccgtggt cgagaagctt cgggccgccg gcctcaactt cgacggtccc gagcgtccgg
     2821 atgtgcccca gacgctcgac ggcatctcca tcgtggtgac cggcacgctc gacggcttca
     2881 cccgggagga gatcgaggag gcgatcaagt cccgcggcgg caaggccccc ggcagcgtct
     2941 ccaagaagac caccgcggtc gtcctcggcc gcgacccggg tcaggccaag gccaccaagg
     3001 ccgaggaact cggcatcccg aggatcgacg aagccgcttt ccgccacctc ctcgacaccg
     3061 gcgagctccc tacgtgacgt tgaagcacca gtcgacgtct cggctgtcgg cccggctctc
     3121 ctcgacccgc cagacgacgg cggtgatgca gttctcgccg gcctcgaagc gctcgacagc
     3181 cttgccgtca ccggccgtgt agaggagctg gccgagcgac gccaggttgg cgtcgtcgat
     3241 ctggtcctcg gggatctcca cgccgttgat gacgagtacg ccggtccacc cgggccggag
     3301 gtcgacaccg acggcctcct ggcgcaggat ctcgctcccc cggggcggga tgagctgctc
     3361 gacggggccg gcctcggtga cggcgatgtc gttggttccg tcgtcggtgt cgaccagggt
     3421 gaaggcgaag acgatccccg ccaggcaggc gacgacggcg aggacgacca ggacacggcg
     3481 cagcgagtcg gacaccgcca cagtgtgtgc cgcggacggc ccgaaccgca cggtggccct
     3541 ctcacgggag actccccgtc ggaggcgacc ccagccttta ccctcggagg catggccatg
     3601 acccgcatga acgaccacgt cggtcgcgtc ctcgcgggtc gctaccggct gctggcgccc
     3661 atcggggccg gcgggtcggc ccaggtcttc ctggccgagg acacccgact caagcgccgg
     3721 gtggccgtca agctcctcca tgagtcctat gcccgggacg aggccttcat ccgccgcttc
     3781 cggtcggagg cgcagaccgc ggccggcctc aaccatccca acctcctcgc cgtgttcgac
     3841 tggggcgagg acgaggtccc gttcctggtc acggagttca tcggcggagg ctcgctccgg
     3901 gccatgctcg accaggggca gcggctcagc atctcccaag ccaccctcat cggcctcgag
     3961 gcggccaaag gtctcgacca cgcccatcgg cagggcctcg tgcaccggga cctgaagccg
     4021 gccaacctcc tgttcggcga ggacgagcgc ctgcgcatcg ccgacttcgg gctcgcccgc
     4081 gcgttggccg acgccgccgt caccgaaccg tccggggcag tgctgggcac cgcccgctac
     4141 gcgtcccccg aacaggcgca gggtcgaccg ctcgacggga gcagcgacat ctactccctc
     4201 gctctcgtgc tcatcgaggc ggtcaccggc caggtgccct tctccgtgga cacgaccatc
     4261 ggcacgctga tggcccgggt ggccgagccc gtccccgtca gcaaccgcct cgccgtcctc
     4321 cagccgatcc tgcgggccgc cggcgcggtc gagcccgagg accggcccga cgccgcccgc
     4381 ctggccacga tgctgatcaa ggcctctgag gagctcgacc ctccggagcg gctgcccctg
     4441 gccggggcca tcgtgctggc ggacaccgcc gtcgagatcg accgcgatcc gacgatgctg
     4501 cccgagccca gcccaggcgg caccgtcttc ccggacgagg ctcccgtacc gccggccgag
     4561 atcgccggcc ccggccgact gggtcgccgg cggcagcggc gggcggccag gaaggcggcg
     4621 aggggtgatc gccgcaccgg ccgccgggtg ctgatcggcg tcctgacggt cgtgctgctc
     4681 gccgccgtag ccgccggcgg ctacgccgcc taccaggtcc tgcgcccgga gcacgaggtc
     4741 cccgacctgg ccgagcagac ggtgggcaac ctggacgacc tggtgggtga tctcggcttc
     4801 gacgtccaac tcgtcggcgt gcgccggaac ggggcgccgg ccgggaccgt ggtcgaacag
     4861 gaccccgccg agggcgagaa gctccgagag ggtcagaccc tcaccgtcac cgtgtcccgc
     4921 ggccccgagt tcctcgacgt tcccgccaac ctgaccaaca tcacccaagc ccagtccgag
     4981 gcggccatcg aggccgccgg gttccgggtc ggtcacgtct cggagaagta caacgagctg
     5041 gtcgtggccg ggatcgtgca gtcggtcatc acccccttcg acgaggtgcc ccgcaacagc
     5101 gccatcgacc tcgtcgtgtc cctcggtccc cggc
//
LOCUS       DASTFV010000148         5057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_515366, whole genome shotgun sequence.
ACCESSION   DASTFV010000148 DASTFV010000000
VERSION     DASTFV010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515366"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFP02_02765"
     CDS             <1..479
                     /locus_tag="VFP02_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET9541975.1"
                     /translation="NPAGVRRQVVGVADMSVAEKLLRVLEAAGGVRALVVHGADGLDE
                     LTTTTTSSVFELRDGEVSSYQVDPTAFGLATAAPDDLLGGTADVNADSARRVLAGEAG
                     AHRDIVVLNAAAGFLVADRASDLAEGVALAQAAIDDGRAEAKLADVVELSPRLSAG"
     gene            complement(489..965)
                     /locus_tag="VFP02_02770"
     CDS             complement(489..965)
                     /locus_tag="VFP02_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541976.1"
                     /translation="MSELLVALFSIAAVIAVAVIIPIMAVRWRLRRRNRVHPNVPSPA
                     PLTWLVAPSTPARLHRRLQVTVRVASTHTGRQVPLAVAEHAVELDRRVVLAARQPYGT
                     RGALLRDLRAEADELDRLAMAVRRPLDVEAVGDPYERLATLAEAHAELDAIDHTRR"
     gene            complement(972..2642)
                     /locus_tag="VFP02_02775"
     CDS             complement(972..2642)
                     /locus_tag="VFP02_02775"
                     /inference="COORDINATES: protein motif:HMM:NF005907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HET9541977.1"
                     /translation="MFAMMRAVPVPGQATLDDLGTPLSEVTFCVIDLETTGGSAAEDR
                     ITEVGAVLLRGGECLGTFQTLVNPGRTVPPVITVLTGITDSMLVPAPTIDTVLPSLLE
                     FWGDAVLVGHNIGFDLGFLRAACARTDRATPEVPRVDTVALARRLFGDEVRNHRLGTL
                     AEQLRLDHKPSHRALDDALATGDLLHVLLERAARLGVLGLDDLLGLPKIDRHPQAAKL
                     KLTNRLPRRPGVYVFRDRDGRPLYVGKAANLRTRVRSYFSSDRRRKVAQLLRETVRID
                     HTVCDGPMEAEVLEVRLIAEWTPRFNKVGTRAGAYTYLKLTCNEAFPRLSVVRTTRDD
                     GAFYLGPLPSARFAKRVAEAIETSVPLRRCSGRPTGRREAPCAPAQLGVATCPCAGSV
                     TVEEYDRLVQRTLRGLTREPEILLAPLRDRMAALARDQRFEEAADVRERAAALSAALE
                     RQGRLDRLRTAGRVVIEVDDGGGAELVDGHLLRSWAEQPTLGLEPVTPTGSTVVRCDE
                     VDEMLCVSRWLDERAGRFRLTHCEGGLASPYPPLPSFTPGRRRRRPVV"
     gene            2687..2923
                     /locus_tag="VFP02_02780"
     CDS             2687..2923
                     /locus_tag="VFP02_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541978.1"
                     /translation="MTAQRFRCTACGNLTRFDVVLSRRTRAFHHYTVGGELSVEDEEV
                     LDEKLEQVACRWCGNGDHVEPVSEQADAVAEDAT"
     gene            2935..4188
                     /locus_tag="VFP02_02785"
     CDS             2935..4188
                     /locus_tag="VFP02_02785"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET9541979.1"
                     /translation="MDPAAVRPALERALEVARQGVAATPTVAAPKELVRYLDFQKLTG
                     PALAAVQRVLDRDDDFRRRVLASTTPEEIGAASWLALSRPAGWEVLLQTVAEADAHAT
                     GSDAAARQARDLQRALEASERKRAQADEALVRLRDEREAIREELASVRKDRRRAEGTL
                     TTVERRVDDWEQKLDDRETRLTEAQGAREAREHELAETQRLLADAHRELARRPAPLVP
                     GERSVDVGGLREAASRLQRAAATLARSVDGVLAEVPPVPVLIEDGGDASALRTRLPLP
                     GGVVADTVDGARWLLARPGAVLLVDGYNVAKTAWPDDDLEKQRDRLIRALDELIMRTG
                     ATAEIVFDGPEGTVPGVRHGTRSVRVRYSGGALADDVVVDLIDAYPVDRPVVVVSSDR
                     EVRDAARVKAATVIGSDTLIAVFTA"
     gene            4231..4419
                     /locus_tag="VFP02_02790"
     CDS             4231..4419
                     /locus_tag="VFP02_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541980.1"
                     /translation="MRIDCDECVQQHTATCGECVVTFLLGREADDAVVIDVAEERQLR
                     LLQDAGLVPALRHQPRTG"
     gene            4453..>5057
                     /locus_tag="VFP02_02795"
     CDS             4453..>5057
                     /locus_tag="VFP02_02795"
                     /inference="COORDINATES: protein motif:HMM:NF019931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueG-associated DUF1730 domain-containing
                     protein"
                     /protein_id="HET9541981.1"
                     /translation="MPAAALADRLRAVGREAGLDALGFASAEVFSSTREDLSNRKAAG
                     LHGGMQFTYRDPERSTDPRRILPGARSLVVGARSYRRAAPGPMDVRATGRVAAYAWRD
                     HYADLGAGLTAVAEVLRADGWTARVVADDNALVDREAAVRAGLGWYGKNSTVLLPGAG
                     SEFVLGSVVTDAELPVAAERVADGCGACRRCVDACPTGAIVA"
BASE COUNT          671 a         1775 c         1964 g          647 t
ORIGIN      
        1 ccaaccccgc cggcgtccgc cgtcaggtgg tgggagtagc cgacatgagt gtggcggaga
       61 agctcctgcg ggtcctcgaa gccgccgggg gggtgcgggc tctggtcgtg cacggcgccg
      121 acggcctgga cgaactgacc acgaccacca ccagctccgt gttcgaactg cgggacggtg
      181 aggtctcgag ctaccaggtc gatccgaccg cgttcggcct ggccaccgcc gcgcccgacg
      241 acctgctggg tgggaccgcc gatgtcaacg ccgactcggc ccgccgggtg ttggcgggcg
      301 aggccggtgc ccatcgggac atcgtcgtgc tcaacgccgc cgccggcttc ctggtcgccg
      361 accgggcgtc cgacctggcg gagggcgtcg ctctggcgca ggcggccatc gacgacggcc
      421 gcgccgaagc gaagctggcc gacgtggtcg agctcagccc ccgcctgtcc gccggctgac
      481 cccgtcggct aacgacgggt gtggtcgatg gcgtcgagct cggcgtgggc ctcggccagg
      541 gtggccagtc gctcgtaggg gtcgccgacg gcctcgacgt cgagggggcg gcggacggcc
      601 atcgcgaggc ggtccagctc gtcggcctcc gcccggaggt cacggagcag cgctcctcgg
      661 gtgccgtagg gctgccgggc ggcgaggacg acccggcggt ccagttcgac ggcgtgctcg
      721 gccacggcga gcgggacctg acgaccggtg tgggtcgacg cgacccgcac ggtgacctgg
      781 agacggcggt ggaggcgggc cggggtcgac ggggcgacga gccatgtcaa gggcgcgggc
      841 gacggcacgt tgggatggac ccggttacgg cggcggagac gccagcgcac ggccatgatc
      901 gggatgatca ccgcgaccgc gatcacggcg gcgatgctga acagcgcaac cagcagctcc
      961 gacatcaccg ctcagactac cggccgccgc cgccgccgtc cgggcgtgaa gctggggagc
     1021 ggcggatagg gcgaggccag gccgccctcg cagtgggtga gccggaagcg gccggcccgt
     1081 tcgtccaacc accgggagac gcacagcatc tcgtcgacct cgtcgcagcg gaccacggtg
     1141 ctgccggttg gcgtcacggg ctccaggccg agggtgggct gctcggccca cgagcgcagc
     1201 aggtgcccgt cgaccaactc ggcgccaccg ccgtcgtcca cctcgatcac gacccgaccg
     1261 gcggtccgca gccggtccag tcggccttgc cgctccaacg cggcactgag cgcggcggcc
     1321 cgctcccgga catcggcggc ctcctcgaag cgctggtccc gggccagcgc ggccatgcga
     1381 tcacgcaagg gggccagcag gatctcgggc tcgcgggtca ggccccgcag cgtgcgttgg
     1441 acgagccggt cgtactcctc gaccgtcacc gaccccgcgc acgggcaggt cgccacgccg
     1501 agctgggcgg gggcgcaggg cgcctcccgg cggccggtgg gtcgtcccga gcaccggcgc
     1561 agcggcaccg acgtctcgat ggcctcggcc acccgcttcg cgaagcgggc gctgggcagc
     1621 ggcccgaggt agaaggcgcc gtcgtctcgg gtggtgcgga cgaccgagag tcgggggaag
     1681 gcctcgttgc aggtcagctt caggtaggtg taggcccctg cccgggtccc gaccttgttg
     1741 aagcgagggg tccactcggc gatgagtcgg acctcgagga cctcggcctc catggggccg
     1801 tcgcagacgg tgtggtcgat gcgcaccgtc tcccgcagga gctgggccac cttccgccgg
     1861 cggtcgctgg agaagtagct gcggacccgg gtgcgcaggt tggcggcctt gcccacgtag
     1921 aggggccggc cgtcccggtc ccggaagacg tagacgcccg gccggcgggg gagccggttg
     1981 gtcagcttga gcttggccgc ctgggggtga cggtcgatct tggggaggcc cagcaggtcg
     2041 tcgaggccga gcacgccgag ccgggccgcc cgctccagga gcacgtggag gaggtcgccg
     2101 gtggccaggg cgtcgtccag tgcccgatgg ctgggcttgt ggtcgagccg aagctgctcg
     2161 gccagcgtcc cgagcctgtg gttgcggacc tcgtcgccga acaggcggcg ggccagggcc
     2221 acggtgtcga cgcgggggac ctcgggcgtg gcccggtcgg tgcgcgcgca ggccgcccgc
     2281 aggaagccga ggtcgaagcc gatgttgtgg ccgaccagga cggcgtcgcc ccagaactcg
     2341 agcagcgagg ggagcacggt gtcgatggtc ggagccggca ccagcatcga gtcggtgatg
     2401 ccggtcagca ccgtgatgac cggcggcacc gtgcgccccg ggttcaccag cgtctggaac
     2461 gtcccgaggc actcgccgcc ccggaggagc acggcaccga cctcggtgat gcggtcctcg
     2521 gcggccgaac caccggtcgt ctcgaggtcg atgacgcaga aggtgacctc ggacaggggg
     2581 gtgccgaggt cgtcgagggt ggcctgtcct ggtaccggca ccgcccgcat catggcgaac
     2641 atctgttcgt cacgtcaagc atggtgcccg tcggtacggt ggcggcgtga ccgcccagcg
     2701 cttccgctgc accgcgtgcg ggaacctgac ccggttcgac gtggtcctca gtcgccggac
     2761 ccgggccttc caccactaca cggtcggcgg cgagctgtcc gtggaggacg aggaggtcct
     2821 ggacgagaag ctggagcagg tcgcctgtcg ttggtgcgga aatggcgacc acgtcgagcc
     2881 cgtgtccgag caggccgatg ccgtcgccga ggacgcgacc tgagcggtcc tggagtggac
     2941 ccggccgccg tgcgcccggc gctggagcgg gccctcgagg tggcccgtca gggcgtggcg
     3001 gcgacgccga cggtggcggc gcccaaggag ctggtccgct acctcgactt ccagaagctc
     3061 accggcccgg cgctggcggc ggtccagcgg gtcctcgacc gggacgacga cttccgccgt
     3121 cgggtgctgg cttcgacgac gcccgaggag atcggcgccg cgagctggct ggcgctgagc
     3181 cgtcccgccg gctgggaggt cctgctccag accgtggccg aggccgatgc ccacgccacc
     3241 gggagcgacg ccgccgcccg ccaggcgcgc gacctgcagc gggccctgga ggcgtcagag
     3301 cgcaagcggg cccaggccga cgaggcgttg gtccgcctgc gggacgagcg cgaggcgatc
     3361 cgcgaagagc tggcctcggt gcgcaaggac cggcgtcggg ccgagggcac cttgaccacc
     3421 gtcgagcgtc gagtcgacga ctgggagcag aaactcgacg accgggagac ccgcctgacc
     3481 gaggcgcagg gagcacggga ggcccgggag cacgagctgg ccgagaccca gcggctgctg
     3541 gccgacgccc accgcgagtt ggcgcgccgt ccggcgccgt tggtgcccgg cgaacgctcc
     3601 gtcgacgtcg gcggcctccg agaggcggcc tcccgcctgc agcgggccgc ggcgaccctg
     3661 gcccgctcgg tggacggcgt gctggcggag gtgccgcccg tgccggtgct catcgaagac
     3721 gggggcgacg ccagcgcact ccgcacccga ctgccgttgc ccggtggcgt cgtggccgac
     3781 acggtcgacg gggcccggtg gctgctggcc cggcccgggg ccgtgctgct ggtcgacggc
     3841 tacaacgtgg ccaagacggc ctggccggac gacgacctgg agaagcagcg ggaccgcctg
     3901 atccgggcct tggacgagtt gatcatgcgc accggggcga ccgccgagat cgtcttcgac
     3961 ggcccggagg gcacggtgcc gggagtccgg cacggcaccc gctcggtccg ggtccgctac
     4021 agcggcggcg ccctcgccga cgacgtcgtg gtcgacctga tcgacgccta tccggtagac
     4081 cgaccggtcg tcgtggtctc cagcgaccgc gaggtccggg acgccgcccg ggtcaaggca
     4141 gccaccgtga tcggctcgga caccctcatc gccgtcttca ccgcctgacc ggcgggctgg
     4201 ctgtcatacc cccttcgtac tgttcggggc atgaggatcg actgcgacga atgcgtgcag
     4261 cagcacacgg cgacctgcgg tgagtgcgtc gtgacgttcc tgctggggcg ggaggcggac
     4321 gacgccgtcg tcatcgacgt ggccgaggag cgccagctgc gcctgctcca ggacgccggc
     4381 ctggtccccg ccttgcgcca ccagccccgg acgggctgag gactccaccg gccgaggtgc
     4441 gagcatgggc gggtgcccgc cgctgccctc gccgaccgcc tgcgggccgt ggggcgggag
     4501 gcagggctgg acgccctggg ctttgcctcc gccgaggtgt tcagctcgac ccgcgaggat
     4561 ctgagcaacc gcaaggccgc gggccttcac ggcggcatgc agttcaccta ccgggacccc
     4621 gaacggagca ccgatccgcg gcgcatcctt cccggtgccc gcagtctggt cgtgggcgcc
     4681 cgctcgtacc gccgggccgc gccgggaccg atggacgtcc gggcgaccgg tcgggtcgcg
     4741 gcgtacgcgt ggcgggacca ctacgccgac ctcggggcgg gcctgaccgc cgtcgccgag
     4801 gtgctccggg ccgacggctg gacggcgcgg gtcgtggccg acgacaacgc cctcgtcgac
     4861 cgggaggcgg cggtgcgagc cggactcggc tggtacggca agaacagcac ggtgctgctc
     4921 cccggtgcgg gcagcgagtt cgtcctcggc tccgtcgtga ccgacgccga gctccccgtc
     4981 gccgccgagc gggtggctga cggctgcggg gcgtgccggc gttgcgtcga cgcctgcccg
     5041 acgggcgcca tcgtcgc
//
LOCUS       DASTFV010000149         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_519982, whole genome shotgun sequence.
ACCESSION   DASTFV010000149 DASTFV010000000
VERSION     DASTFV010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519982"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..600
                     /gene="pyrR"
                     /locus_tag="VFP02_02800"
     CDS             46..600
                     /gene="pyrR"
                     /locus_tag="VFP02_02800"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET9541982.1"
                     /translation="MTPPYGGVFTARSQVMDREDVHRALRRMAHEIIERNEGRLAEVV
                     VIGLQTGGAPMAAALVAALEEIDEAAAPIDMGTIDVALYRDDIGLRPVMPEEVTEIPV
                     DLTERIVVLVDDVLFTGRTIRAALDALTDFGRPRSVQLAVIVDRGHRELPIRPDYVGK
                     NLPTRRDEVVDVSIDGVELGEMVK"
     gene            597..1538
                     /locus_tag="VFP02_02805"
     CDS             597..1538
                     /locus_tag="VFP02_02805"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET9541983.1"
                     /translation="MSRHLLSIEDLGAGGIDEILQLTDTFVEVSARPIARVPALRGKT
                     VAFCFYEDSTRTRVSFETAAKRLSADTVNFSIAASSVNKGESVRDTVETICAMGVDGL
                     VVRHRSPGVPWQITRWVDATVINAGDGGHEHPTQALLDAYTIRQELGSVEGRRIAIVG
                     DIKHSRVARSNVAAFTALGAEVTLVAPPTLLPPRLDDWPVEVSSDLDAVLPKVDVVYL
                     LRMQKERMTESLIPSLREYTAGYGLTRRRVDLLSDDAVVMHPGPMNRGVEIAAEVADL
                     PRAVVTNQVRNGVAVRMAVLYLLLGSGVDLKPEVVDG"
     gene            1531..>2476
                     /locus_tag="VFP02_02810"
     CDS             1531..>2476
                     /locus_tag="VFP02_02810"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET9541984.1"
                     /translation="MADADLVIRGGTVVDADGSRRADVAVADGVIVAVGDGLSGGREL
                     DAGGCIVGPGLVDLHAHLREPGKEEAETVETGARAAARGGFTAVVAMPNTTPAIDSAS
                     VVREVQALARDALCAVHPAAALTVGRAGEALTPMAELADLGVRLFTDDGNGVQDDRLM
                     RRAMEYATGLAHDVVLAQHCEVEALAEGGHMHEGAWSSRLGIPGIPAAAEELMVARDL
                     ALARLTGARVHFQHMSTAGSVELIRRAKDDGVRVTAEVAPHHLTLTDAEVAAFDAVFK
                     VNPPLREQSDVDAVRAGLIDGTLDAVATDHAPHTQEDKE"
BASE COUNT          364 a          913 c          868 g          331 t
ORIGIN      
        1 gtacggacag agaggaccac cggcttggcc gaacgagaac gcaagatgac gcccccttat
       61 gggggcgttt tcactgcccg gtcacaggtc atggaccgcg aggacgtcca ccgggcgctg
      121 cggcggatgg cccacgagat catcgagcgc aacgagggcc gcctggccga ggtggtcgtg
      181 atcgggctcc agacgggcgg ggccccgatg gcggcggcgc tggtcgcggc cctcgaggag
      241 atcgacgagg ccgccgcccc catcgacatg ggcaccatcg acgtcgccct gtaccgggac
      301 gacatcgggc tccggccggt catgcccgaa gaggtcaccg agatccccgt cgacctgacc
      361 gagcgcatcg tggtgctggt cgacgacgtg ctcttcaccg gccgcaccat ccgcgccgcc
      421 ctcgacgccc tcaccgactt cggtcggcct cgctcggtcc agctcgcggt gatcgtcgac
      481 cggggtcacc gggagctgcc catccgcccc gactacgtgg gcaagaacct gcccacccgc
      541 cgcgacgagg tcgtggacgt ctccatcgac ggggtcgaac tgggcgagat ggtgaaatga
      601 gccggcacct cctgtccatc gaggacctgg gggcaggggg catcgacgag atcctccagc
      661 tgaccgacac cttcgtcgag gtctctgccc gcccgatcgc ccgggtcccc gccctgcggg
      721 gcaagaccgt ggccttctgc ttctacgagg actccacccg cacgcgggtc agcttcgaga
      781 cggcggccaa gcgcctgtcc gccgacacgg tcaacttctc catcgccgcc tcctcggtca
      841 acaagggcga gtcggtccgg gacaccgtcg agaccatctg cgccatgggg gtcgacggcc
      901 tggtcgtccg ccaccgctcg ccgggggtgc cctggcagat cacccggtgg gtggacgcca
      961 cggtgatcaa cgccggggat gggggccacg agcaccccac ccaggctctg ctggacgcct
     1021 acacgatccg ccaggagctg ggctcggtgg agggccgcag gatcgccatc gtgggcgaca
     1081 tcaagcactc ccgggtggcc cgctccaacg tggccgcctt caccgcgctg ggggccgagg
     1141 tcaccctggt ggcaccgccg accctgctcc cgccccgcct cgacgactgg ccggtggagg
     1201 tcagcagtga cctcgacgcc gtcctgccca aggtggacgt cgtctacctc ctccgcatgc
     1261 agaaggagcg catgaccgag tccctgatcc cctcgctgcg ggagtacacc gcgggctacg
     1321 gcctgacccg gcggcgggtc gacctgctga gcgacgacgc ggtggtcatg caccccgggc
     1381 ccatgaaccg gggcgtggag atcgccgccg aagtggccga cctgccgcgg gccgtggtca
     1441 ccaaccaggt ccgcaacggc gtggccgtgc ggatggcggt gttgtacctg ctcctcggct
     1501 ccggcgtgga cctgaagccg gaggtcgtcg atggctgatg ccgatctcgt catccggggc
     1561 gggacggtcg tggacgccga cggctcgcgg cgggccgatg tggccgttgc cgacggcgtc
     1621 atcgtggcgg tcggcgacgg cctgagcggc gggcgggagc tggacgccgg gggctgcatc
     1681 gtcggcccgg gcctggtcga ccttcacgcc cacctccggg agccgggcaa ggaggaggcc
     1741 gagaccgtgg aaaccggtgc tcgggccgcg gcccgaggcg gcttcactgc cgtggtggcc
     1801 atgcccaaca cgacaccggc catcgacagc gcctccgtgg tccgcgaggt ccaggccctc
     1861 gcccgcgatg ccctgtgcgc cgtccacccc gccgcggcac tgaccgtggg ccgggcgggc
     1921 gaggcgctga ccccgatggc cgagctggcc gacctcggcg tccgcctctt caccgacgac
     1981 ggcaacggcg tgcaggacga ccgcctgatg cgccgcgcca tggagtacgc caccggactg
     2041 gcccacgacg tggtcctggc ccagcactgc gaggtcgagg ccctggccga gggtggccac
     2101 atgcacgagg gcgcctggtc cagccgtctc ggcatccccg gcatcccggc ggcggcggag
     2161 gagctcatgg tcgcccgcga cctggcgctg gcccgcctga ccggagcccg cgtgcacttc
     2221 cagcacatga gcacggccgg ctcggtcgag ctcatccgac gggccaagga cgacggcgtc
     2281 cgggtcacgg ccgaggtcgc cccgcaccac ctcaccctga ccgacgcgga ggtggccgct
     2341 ttcgacgccg tgttcaaggt gaacccgccg ctgcgcgaac agtccgacgt cgacgccgtc
     2401 cgggccggtc tgatcgacgg caccctcgac gccgtcgcca ccgaccacgc cccccacacc
     2461 caggaggaca aggaaa
//
LOCUS       DASTFV010000150         4533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_522862, whole genome shotgun sequence.
ACCESSION   DASTFV010000150 DASTFV010000000
VERSION     DASTFV010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522862"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFP02_02815"
     CDS             <1..308
                     /locus_tag="VFP02_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9541985.1"
                     /translation="RDLAVLRTVGFTRRQVLAVVAWQATALVLAAALVAVPVGIVLGR
                     WAWLIFADELRVVGRPVVPALAVIGATLGAILMANVVAVATGRRSARASAATALRAE"
     gene            389..3886
                     /locus_tag="VFP02_02820"
     CDS             389..3886
                     /locus_tag="VFP02_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET9541986.1"
                     /translation="MAAGTATQESPPSMCGIIAVVRRPSERVPASSAELLALVEQASA
                     ASAGFADLAPGPALVDLLDAVAAPLVQADALLRGSPGVRTLLADPALTPTLDRLTAGI
                     EGRADAVEGALDHAESLGSTAELELVNAALVRLRDAAWAVRRDRLPTAARVAELAGSD
                     PGPAAIEAFLSLQQVLSAIDRLEVRGRDSAGIHVLVHGHGLDLEAPSVRALLDGRDDD
                     PRFVSGAVRVAHGHLAFVYKAAAEIGELGDNTAALRAAIADDALLGLALSGPTAEALV
                     LGHTRWASVGIISEPNAHPVCGEESDGVGRPYVAAALPGVVDNHADLRAEEGLVVAAE
                     ITTDAKVIPALTARRLAEGDGLDEAFRRTVATLEGSVAIAATNGVAPERLHLALRGSG
                     QALYVGLTEDAYVVASEPYGLVEETSTYVRMDGETPANPDNPTASRGQVLVLDGARAG
                     TVEGIARVAYDGTELPVAATDLETAEITTRDIDRGDFPHFLLKEMTEAPASFRKTLRG
                     RLTERDGRATVELGPETLPDDLRAQLREGRIHRVLVTGQGTAAVAGQSLAATLEALAP
                     ESPVRVEAVVATELSGFELAEDMSDTLVVAISQSGTTTDTNRTVDLVRSRGGAVLAIV
                     NRRGSDLTDKADGVLYTSDGRDVEMSVASTKAFYAQVAAGVLLAAALAEELGAPDTGE
                     RTVMLAALRGLPDALTRVLEDRERIGETARELAPGRRYWAVVGNGPNRIAAAEVRIKL
                     SELCYKSIACDVTEDKKHIDLSSEPLILVCAAGLTGSNADDVAKEVAIYRAHKAAPVV
                     IATEGEGRYTSALQALTVPPTHPRLAFVLAAMVGHLFGYEAALAIDEGARPLREARAA
                     IEEAVGSRVEGADAEVLLEKLRPEFEPRATRFADTLRSGAYDGHLEASTASRAGALFR
                     YALGVSPLDGYQGEYGKVGTPGVIVEDLTAALTRAIEELTRPIDAIKHQAKTVTVGIS
                     RTDETLLQVPLVRAVMAAGAPRDGLTYHALRVLADLDPAVAEVTGWTRYRIEGRPESG
                     EATIVALDRGGVAAGIPTRTEQDPRLRGTKNRVASERQVLVATGRRDGRPIVLVPESK
                     DGQATGLTLLHVRFRDRLSAITARGVLQGYRHRFALLRDAVTETEPTFRDDLLAEIDV
                     ADLLVASISSLADRFRSES"
     gene            3892..4263
                     /locus_tag="VFP02_02825"
     CDS             3892..4263
                     /locus_tag="VFP02_02825"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET9541987.1"
                     /translation="MVVGLGTDLVDLDRFRLAAERTPGILTRYFTEGERAYAERRQDP
                     TERYAARFAAKEAVMKALGVGLGSCELAEIEVVRDEDSGAPDLVLHGKAAALAADRGV
                     TAWKLSLTHTGTSAHAIVIAL"
     gene            4311..>4533
                     /locus_tag="VFP02_02830"
     CDS             4311..>4533
                     /locus_tag="VFP02_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541988.1"
                     /translation="MGPVVRNATVEDLDELMALREAVAGEGRWIGAELPLDVERVRRA
                     FWESAERGSLFVAEVDGEIVGMLGMSLPPF"
BASE COUNT          590 a         1686 c         1654 g          603 t
ORIGIN      
        1 gacgggacct cgccgtcctg cggacggtcg ggttcacccg gcgccaggtc ctggccgtgg
       61 tcgcgtggca ggcgacggcc ctggtgctcg cggccgccct cgtcgccgtc ccggtcggca
      121 tcgtcctggg ccggtgggcg tggctgatct tcgccgacga gctgcgggtg gtgggccggc
      181 ccgtggtgcc cgccctcgcc gtgatcggcg cgaccttggg tgccatcctg atggccaacg
      241 tcgtcgccgt ggccaccggt cgccggtcgg cccgcgcgtc ggccgcgacc gccctgcgag
      301 cggagtgacc gccgagtcct ggctctgacc gaccgcggtc cctccgggcg aggggttcgt
      361 acactgtggc ccgcgccacc ggtctgcggt ggctgcgggc accgccaccc aggagtcacc
      421 accgtcaatg tgcgggatca tcgctgtcgt ccgacgcccc tctgagcgtg tcccggcctc
      481 cagcgccgaa ctgttggccc tcgtcgagca ggcctcggcc gcctcggccg gattcgccga
      541 cctggctccc ggtccggcct tggtcgacct gctcgacgcc gtggccgcgc cgctggtgca
      601 ggccgacgcc ctgctcaggg gatcgcccgg ggtgcggacc ctgctcgccg accccgccct
      661 gacccccacg ctggaccgcc tgacggccgg gatcgagggc cgagccgacg ccgtggaagg
      721 ggcgctcgac cacgccgagt cgctgggctc gaccgccgag ctggaactgg tcaatgccgc
      781 cctggtccgg ctccgggacg cggcgtgggc cgtccggcgg gaccggttgc cgaccgcggc
      841 gcgggtcgcc gaactggccg ggtcggatcc cggcccggcg gccatcgagg cttttctctc
      901 cctccagcag gtgctctcgg ccatcgaccg cctggaggtc cggggtcggg acagcgccgg
      961 catccacgtg ctcgtccacg gccatggcct ggacctggag gccccgtcgg tgcgcgccct
     1021 cctcgacggg cgggacgacg acccccggtt cgtctccggt gccgtgcggg tcgcgcatgg
     1081 ccacctcgcc ttcgtctaca aggcggcggc ggagatcggc gagctggggg acaacacagc
     1141 ggcgttgcgg gccgcgatcg ccgacgacgc cctgctgggc ctcgccctga gcgggccgac
     1201 ggccgaggcc ctcgtcctcg gtcacacccg ctgggccagc gtgggcatca tctccgagcc
     1261 caacgcccac ccggtctgcg gcgaggagtc ggacggggtc ggccgccctt acgtggccgc
     1321 cgccctcccc ggcgtcgtcg acaaccacgc cgacctgcgg gccgaggagg gcctggtggt
     1381 cgcggccgag atcacgaccg acgccaaagt gatccctgcc ctcaccgccc gtcgcctggc
     1441 ggagggcgat ggcctcgacg aggccttccg gcgcacggtg gccaccctcg aaggctcggt
     1501 ggccatcgcg gccaccaacg gcgtggctcc ggagcgactg cacctggccc tgcgggggag
     1561 cggccaggcc ctgtacgtgg ggttgaccga ggacgcttac gtcgtggcca gtgagcccta
     1621 cgggctggtc gaggagacgt cgacctacgt gcggatggac ggcgagacgc ccgccaaccc
     1681 ggacaacccg acggccagcc ggggccaggt cctggtcctc gacggcgccc gagcggggac
     1741 ggtcgagggc atcgctcggg tggcttacga cggcaccgag ctgcctgtcg ccgccaccga
     1801 cctggagacg gccgagatca cgacccggga catcgaccgg ggtgacttcc cgcacttcct
     1861 gctcaaggag atgaccgagg ccccggcctc gttccgcaag accctgcggg ggcgcctgac
     1921 cgaacgggac ggccgggcca ccgtggagct cgggccggag acgctgcccg acgacctgcg
     1981 ggcgcagctg cgggagggcc gcatccatcg ggtcctcgtc accggccagg gcacggccgc
     2041 cgtcgccggg cagagcctgg cggccaccct cgaggcgctc gcccctgaaa gcccggtgcg
     2101 ggtggaggcc gtcgtggcga ccgaactgtc cgggttcgag ttggccgagg acatgtcgga
     2161 caccctggtc gtggccatct cccagtccgg gaccaccacc gacaccaacc ggacggtcga
     2221 cctggtccgc tcccgagggg gcgccgtgct ggccatcgtc aaccgtcggg gcagcgacct
     2281 gaccgacaag gccgacggcg tcctctacac ctccgacggg cgggacgtgg agatgagcgt
     2341 cgcctccacc aaggccttct acgcccaggt ggccgcgggc gtcctgctcg cggccgccct
     2401 ggcggaggag ctcggggcgc ccgacacggg tgagcggacg gtcatgctgg ccgcgctgcg
     2461 gggcctgccc gacgcgctca cccgggtcct cgaggaccgg gagcgcatcg gggagaccgc
     2521 gcgggagctg gccccggggc ggcgctactg ggccgtggtc ggcaacgggc ccaaccgcat
     2581 cgccgccgcc gaggtccgga tcaagctgtc ggagctctgt tacaagtcga tcgcctgtga
     2641 cgtcaccgag gacaagaagc acatcgacct gtcctccgaa ccgctcatcc tggtctgcgc
     2701 ggcgggcctg accggttcca acgccgacga cgtggccaag gaagtggcca tctaccgggc
     2761 ccacaaggcg gcccccgtgg tcatcgccac ggagggcgag ggccggtaca cctcggccct
     2821 gcaggccctg accgtcccgc ccacccaccc ccgcctggcg ttcgtgctcg ccgccatggt
     2881 cggtcacctc ttcggctacg aggcggcgct ggccatcgac gagggggctc gccccctgcg
     2941 ggaggctcgg gccgcgatcg aggaggccgt ggggagccga gttgaaggag cggacgccga
     3001 ggtcctgctc gagaagctcc gtcccgagtt cgagccccga gccactcgct tcgccgacac
     3061 cctgcgcagc ggggcctacg acggccacct ggaggccagc acggcgtcgc gtgccggggc
     3121 cctcttccgc tacgccctcg gtgtctcgcc cctggacggc tatcagggcg agtacggcaa
     3181 ggtgggcacg ccgggcgtca tcgtcgagga cctcaccgcg gcgctgaccc gtgccatcga
     3241 ggagctgacc cggcccatcg acgccatcaa gcaccaggcc aagacggtga cggtgggcat
     3301 ctcccgcacc gacgagaccc tgctccaggt gcccctggtc cgggccgtca tggccgccgg
     3361 agcgccccgg gacggcctca cctaccacgc cttgcgcgtg ctggccgacc tcgatccggc
     3421 ggtggccgag gtcaccgggt ggacccggta ccgcatcgag ggccgcccgg agagcggcga
     3481 ggcaaccatc gtggccctcg accggggtgg cgtggccgcc ggcatcccga cccggaccga
     3541 gcaggacccg cgcctgcggg ggaccaagaa ccgggtggcc tccgagcgcc aggtgctggt
     3601 ggccaccggc cggcgggacg gacgacccat cgtgctcgtc cccgaatcca aggacggtca
     3661 ggcgaccggg ttgaccctgc tccacgtccg cttccgggac cgcctgtcgg ccatcaccgc
     3721 ccgaggggtc ctccagggct accgccaccg gttcgccctc ctgcgggacg ccgtcaccga
     3781 gaccgagccc accttccgcg acgacctgct ggccgagatc gacgtggccg acctgttggt
     3841 ggcctcgatc agctcgctgg ccgaccgctt ccgcagcgag agctgaggcc cgtggtcgtc
     3901 ggcctcggca ccgacctggt cgacctcgac cggttccggc tggccgccga gcgcaccccc
     3961 gggatcctca cccggtactt caccgagggc gaacgggcct acgccgagcg gcggcaggac
     4021 cccaccgagc gctacgccgc ccggttcgcg gcgaaagagg cggtgatgaa ggccttgggc
     4081 gtggggctcg ggtcctgcga gctggccgag atcgaggtcg tccgggacga ggactccggc
     4141 gcccctgacc tggtcctcca cggcaaggcc gcggcgctgg ccgccgaccg cggcgtcacc
     4201 gcttggaagc tgagcctgac ccacaccggc accagcgccc acgccatcgt catcgccctc
     4261 tgaccacgaa atcgggcgcc aggacggctc gggtggtcga gactggcggg atggggccgg
     4321 ttgtgcggaa cgcgacggtg gaggacctcg acgagctgat ggcgttgcgg gaggccgtgg
     4381 cgggggaggg ccggtggatc ggggccgagc tgccgctgga cgtcgagcgg gtccgtcgcg
     4441 ccttctggga gagcgccgag cgaggatctc tcttcgtggc cgaggtcgac ggcgagatag
     4501 tgggcatgct cgggatgagc ctgccgccct tcc
//
LOCUS       DASTFV010000151         2486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_523214, whole genome shotgun sequence.
ACCESSION   DASTFV010000151 DASTFV010000000
VERSION     DASTFV010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523214"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VFP02_02835"
     CDS             complement(<1..719)
                     /locus_tag="VFP02_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9541989.1"
                     /translation="MSGGLTWYLARSLGIVAYALLTASMLWGLWLSTRTPARQGRAWL
                     LDVHKVLGGLGVLFVLGHLGALVADSYIDFGVVDLLVPFASGWQPRAVAWGVLALYLL
                     AAVEVTSLLRRTLTRRSWRKIHLAAFAAYLMATAHFLTAGTDAGGRLVLSLTFGGVAL
                     VATGTLYRVVQHLTRPTRVHTPAKERSWSPSPPPTTPPSRPARLPPAPPQAASPPRPR
                     LPVPSHPPGAPVAVRSLASAGG"
     gene            complement(704..1618)
                     /locus_tag="VFP02_02840"
     CDS             complement(704..1618)
                     /locus_tag="VFP02_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET9541990.1"
                     /translation="MSEAVHHHRFAAMGTTAHIVVVGGTFDHLEQARRRIGQLERRWS
                     RFLPDSEVSTINASDGLPLIVSRDTTLLVERSVQAWRATAGRFDPTVHDAVVAAGYDG
                     AFAGGPVPAPLPAVAPSPGLHDVVVDRGTGLVWVPAGVHLDPGGIGKGLAADLVVTEL
                     RAAGVGGACVNLGGDLRVDGLAPQGDTWSVAIDDPRDRGRELTRIPLSSGGVATSSRL
                     DRSWEIDGVPAHHLIDPASGRPADTEIVAVTAVAATAWWAEVTATWAFLGDGAPRALG
                     ATEVLLVTADGTTRSTRRLQEVASCPAA"
     gene            1716..2372
                     /gene="merB"
                     /locus_tag="VFP02_02845"
     CDS             1716..2372
                     /gene="merB"
                     /locus_tag="VFP02_02845"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HET9541991.1"
                     /translation="MDTEDLRLAVYHHLADTGRAPVTGALAAVFDVDEDEVVEGLEAL
                     HDARHIVLRDGMIVMAHPFATIPLGFSVMGADTLWWGGCAWDSFALPHLLPDEDEVLV
                     ATTCPGCGAALSWVVGTDAPPEGDEVAHFLVPTSHIWDDVVHTCGHQRLFCSAACVDR
                     WLTDSGDDRGYVMDLPTLWRLARGWYAGRLERGYVRREPADAHAYFAEVGLEGEFWGL
                     "
BASE COUNT          350 a          830 c          989 g          317 t
ORIGIN      
        1 ccgccggctg acgccagcga ccggacggcg acgggcgccc cgggtgggtg cgacggcacg
       61 gggagccggg ggcggggtgg ggaagccgcc tgtggcggtg ctggggggag ccgcgccggg
      121 cggctggggg gggttgtcgg gggtggggat gggctccatg agcgctcctt ggcgggggtg
      181 tggacacggg tcggacgggt caggtgctga acgacgcggt agagcgtccc ggtggctacg
      241 agggccacgc ccccgaaggt gagggacagg acgaggcggc cgccggcatc ggtgcccgcc
      301 gtgaggaaat gggcggtggc catcaggtag gcggcgaagg cggccaggtg gatcttgcgc
      361 cagctccggc gggtgagggt gcggcgcagc agcgacgtca cctcgacggc ggccaggagg
      421 tagagggcga gcacccccca ggccacggct ctcggctgcc agccgctggc gaacgggact
      481 aggaggtcga ccacgccgaa gtcgatgtag ctgtcggcca ccagcgcgcc gaggtggccg
      541 aggacgaaca ggacgcccag gccgccaagg accttgtgga catccaggag ccaggcccga
      601 ccctgacgag cgggtgtccg ggtggagagc cacaggcccc agagcatcga cgcggtcagg
      661 agggcgtagg ccacgatccc gagggagcgg gcgaggtacc aggtcaggcc gccggacacg
      721 aggcgacctc ctgcaaccgg cgggtcgagc gggtggtgcc gtcggcggtg acgaggagga
      781 cctccgtggc accgagggcg cgcggcgccc cgtcgcccag aaacgcccac gtggcggtga
      841 cttcggccca ccacgcggtg gcggccaccg cggtgacggc gacgatctcg gtgtcagccg
      901 ggcgaccgga cgccgggtcg atgaggtggt gggcggggac accgtcgatc tcccagctgc
      961 ggtccagtcg gctgctggtg gcgaccccgc cagagctcag ggggatccgc gtcagctcac
     1021 ggccgcggtc cctcgggtcg tcgatcgcca ccgaccacgt gtcaccctgt ggcgcgaggc
     1081 cgtcgacccg taggtcgccg ccgaggttca cgcaggcccc gcccacgccg gccgcccgga
     1141 gctccgtgac aacgagatcg gcggccaacc ccttgccgat cccgccggga tcgaggtgaa
     1201 cgccggcagg gacccagacg aggccggtgc cccggtccac caccacgtcg tgaagcccgg
     1261 gggagggcgc gacggcgggg agaggggcag ggaccggtcc accggcgaag gcgccgtcgt
     1321 agccggcggc gaccacggcg tcgtgcaccg tgggatcgaa gcgcccggcc gtcgcccgcc
     1381 acgcctgcac gctgcgctcg acgaggaggg tggtgtcgcg gctgacgatc agtgggaggc
     1441 cgtcggaggc gttgatggtc gagacctcgc tgtcggggag gaaccggctc caccggcgct
     1501 ccagctggcc gatgcggcgg cgggcttgtt ccaggtggtc gaaggtgccc ccgaccacga
     1561 cgatgtgggc agtggtcccc atggcggcga accggtggtg gtggacggcc tcggacatgg
     1621 cgctcaccgt cggccctgga tctgcgaacg ggggccgggc gggctgggag gttcctgaga
     1681 atcgtcgaag gccgcggctc gggcagactc gggccatgga caccgaggac ctgcgcctgg
     1741 ccgtctacca ccacctggcc gacacaggcc gggcaccggt caccggtgcg ttggcggcgg
     1801 tgttcgacgt cgacgaggac gaggtggtcg agggcctgga ggcgctgcac gacgcccgcc
     1861 acatcgtcct gcgggacggg atgatcgtca tggctcatcc cttcgccacc atcccactgg
     1921 gcttctcggt gatgggggcc gacaccctct ggtggggtgg ctgcgcctgg gacagcttcg
     1981 ccctgcccca cctgctgccc gacgaggacg aggtcctcgt ggccaccacc tgcccgggct
     2041 gtggggcggc cctgtcctgg gtcgtgggga ccgacgcccc gcccgaaggt gacgaagtgg
     2101 cccatttcct ggtcccgacc agccacatct gggacgacgt ggtccacacc tgcggccacc
     2161 agcgcctctt ctgctcggcg gcctgcgtcg accggtggtt gaccgactcg ggcgatgacc
     2221 ggggctacgt catggatctg ccgaccctct ggcgcctcgc tcgcggctgg tacgccggcc
     2281 gactcgagcg cggctatgtc cggagggagc ccgccgacgc ccacgcctac ttcgccgagg
     2341 tcggcctcga aggggagttc tggggcctct agcccccggt ctcgaagccg gtgaggacgc
     2401 ccgtggcatt ggtgccgatg gcttcgacgg cgtagccgcc ctccatcacg aagagggtcg
     2461 gggcgccgag gtcggccagg cgggcg
//
LOCUS       DASTFV010000152         4388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_527088, whole genome shotgun sequence.
ACCESSION   DASTFV010000152 DASTFV010000000
VERSION     DASTFV010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527088"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..365
                     /locus_tag="VFP02_02850"
     CDS             126..365
                     /locus_tag="VFP02_02850"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9541992.1"
                     /translation="MRSRPPTEICTTGLRSRSSINRGDSGGPLLVRGWWDCAFRPVGV
                     TALGSDSTMDLYAGFTSIPVEAGWIAQSIKALRLG"
     gene            404..871
                     /gene="bcp"
                     /locus_tag="VFP02_02855"
     CDS             404..871
                     /gene="bcp"
                     /locus_tag="VFP02_02855"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HET9541993.1"
                     /translation="MADPTRLEAGAKAPAIALLDQNGEKVKLADFKGRPVLVYFYPRA
                     DTPGCTTQSCGLRDVKDDVGDAVILGISPDLPPKLAKFDEKYGLGFTLLSDPEHAVAD
                     AYGVWGEKKNYGKTYMGIIRSAFLIDEKGKVEQAWYKISPKDTPTKFLAALGS"
     gene            1004..1474
                     /gene="fabZ"
                     /locus_tag="VFP02_02860"
     CDS             1004..1474
                     /gene="fabZ"
                     /locus_tag="VFP02_02860"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET9541994.1"
                     /translation="MSPPLWSPGEPMPRPDAIIPHRHPFLLVDEITEIVPDERATGYW
                     HPTGDEAFFAGHFPDYPVVPGVLLIEALAQVGAVAAMAGETDADAIPFFGGIDKARFR
                     RQVRPGERIDLEVELTQRSKRAGKGQGRATVDGELACETGLFFLAVRRSELPEG"
     gene            complement(1471..2655)
                     /locus_tag="VFP02_02865"
     CDS             complement(1471..2655)
                     /locus_tag="VFP02_02865"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET9541995.1"
                     /translation="MDGRRVVVTGVGVVSAVGIGAEAFWAALLGPAQVGKTREIQDWD
                     PGPWFDGPKDARRADRFTQLAAGAAGLALEQSGDPGGDPDRIGVLVGTGIGGLHTLED
                     QVEVNRVRGPERVSPFLIPMLMPNAAAAGISMRHGFRGPCETTVTACAAGTHSIGNAA
                     RLIATGRCNAVVTGGTESAITQTGVAGFENMTALSSSGVSRPFDRDRDGFVMAEGAGV
                     LVLEELEHARARGATPLAEVLGSGSTADAHHITAPSPGGSGAVTCMELALADAGRSPD
                     SVRQINAHGTSTPLNDAAEAEAIVKVFGAQGPPVTSIKGITGHSLGAAGALEAAAVVL
                     SMQHRLIPPTANHVQAEDDLAPIDVVTGQGREWAPGPTLSNSFGFGGHNGCIVFDAPP
                     AG"
     gene            complement(2658..2894)
                     /gene="acpP"
                     /locus_tag="VFP02_02870"
     CDS             complement(2658..2894)
                     /gene="acpP"
                     /locus_tag="VFP02_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET9541996.1"
                     /translation="MSDDLFERFKGHAVTVLAVDADKVTLEANFADDLDADSLDLVEL
                     VMALEDEFDVSIEEEELEGVATVAQAVDLVRSKL"
     gene            complement(2937..3659)
                     /gene="fabG"
                     /locus_tag="VFP02_02875"
     CDS             complement(2937..3659)
                     /gene="fabG"
                     /locus_tag="VFP02_02875"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HET9541997.1"
                     /translation="MTDAGRVALVTGGNRGIGLACATALAEAGYRVAITHRGEAPDAP
                     GLLPVPCDVTDPATVEAAFAAVEEAFGPVEVLVHAAGINADTLLLMMKEEDFSRVIDA
                     NLTGAFRVSKRAAKRMLRARWGRIILVGSVVGQIGSAGQANYAASKAGLVGFARSLAR
                     ELAPRSITVNVVSPGPIDTDMLRALDDDQRALLTSMVPLGRVGQPEEVAAAVGYLASD
                     AAAYLTGVNLPVDGGLGMGVGS"
     gene            complement(3656..>4388)
                     /locus_tag="VFP02_02880"
     CDS             complement(3656..>4388)
                     /locus_tag="VFP02_02880"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HET9541998.1"
                     /translation="GRRPEDVDALVVATTTPDQTVPATASTVQEILGLDCGAFDLNAA
                     CSGFVYGLVVAHGLLAIGHRCVLVVGADTLSRITDWDDRGTAILFGDGGGAVVLEAVD
                     EDRILAWDLSSDGSGRNLLYADVGGTMKMDGPEIYRRAVRVIVSSAQATLQRAGVEAA
                     DLDLLVPHQANQRIIDAACRRLGVPDEKVVSVLEHTGNTSAASIPHALAEAADQGRVA
                     DGALVLLVGFGAGMTGASTLLRWGR"
BASE COUNT          617 a         1544 c         1600 g          627 t
ORIGIN      
        1 caagggctga cgcacccact ccggttccac gggggcggcc tccgggccgc ccccgtggcg
       61 cgtccggggg ccctccgcgg aacgggccac ggacgaggtg agtcccggcg cccggcgctg
      121 gcaggatgag gtctcggccc ccaaccgaga tctgtacgac gggcctgcgg tcccggtcga
      181 gcatcaaccg tggcgattcc ggtggcccgc tgctcgtgcg gggctggtgg gattgtgcgt
      241 tccggccagt cggcgtcacg gcgctgggct cggacagcac gatggacctc tacgccgggt
      301 tcacgtcgat ccccgtggag gccggctgga tcgcccagtc gatcaaggcg ttgcggctcg
      361 gctgagcgga cgttgtcggg tcgacggcgg ctaccttgcg cccatggctg atcccacacg
      421 actcgaggcg ggggccaagg ccccggcgat cgccctgctg gaccagaacg gcgagaaggt
      481 gaagctggcg gacttcaagg gtcggcccgt cctcgtctac ttctacccgc gggccgacac
      541 gccgggctgc acgacccagt cctgtggcct gcgggacgtg aaggacgacg tcggcgacgc
      601 cgtcatcctc ggcatcagcc ccgatctgcc acccaagctg gccaagttcg acgagaagta
      661 cgggctcggg ttcaccttgc tctccgaccc cgagcacgcc gtggccgacg cctacggcgt
      721 ctggggcgag aagaagaact acgggaagac ctacatgggg atcatccgct cggcgttcct
      781 gatcgacgag aagggcaagg tcgaacaggc ctggtacaag atcagcccga aggacacgcc
      841 gacgaagttc ctggccgcgc tgggctcgtg agccgtcctg cctcgatggg gccgaccgat
      901 cacggtgcgg ccgagttcct ggccgcgttg ggctcgtgag ccgtactacg tcgatggggc
      961 cgaccgatca cggcgcggcc gagttcctga ccgcgctggg ctcgtgagcc cgccgctgtg
     1021 gtcgccgggc gagccgatgc cgcgacccga cgcgatcatc ccgcaccgcc acccgttcct
     1081 cctggtcgac gagatcaccg agatcgtccc cgacgagcgg gccaccggct actggcaccc
     1141 gaccggcgac gaggcgttct tcgccgggca cttccccgac tatcccgtcg tgcccggcgt
     1201 gctgctcatc gaggccttgg cccaggtggg ggcggtggcg gcgatggccg gggagaccga
     1261 cgccgacgcc attccgttct tcggtggcat cgacaaggct cggttccgcc ggcaggtccg
     1321 tcccggcgag cggatcgacc tcgaggtcga gctcacccag cggtccaagc gggcagggaa
     1381 gggtcagggc cgggccaccg tggacgggga gctggcctgt gagaccggcc tcttcttcct
     1441 cgccgtccga cggtcggagc tgcccgaggg ctagccggcc ggcggggcgt cgaagacgat
     1501 gcagccgttg tggccgccga agccgaacga gttcgacagg gtgggccccg gcgcccactc
     1561 ccgtccctgg ccggtgacga catcgatggg cgcgaggtcg tcctcggcct ggacgtggtt
     1621 ggcggtgggc gggatcagtc ggtgctgcat cgagagcacg acggcggcgg cctccaacgc
     1681 cccagcggcg ccgaggctgt ggcccgtgat ccccttgatc gaggtcaccg gcggcccctg
     1741 ggccccgaag accttgacga tggcctccgc ctccgcggcg tcgttgaggg gcgtggaggt
     1801 gccgtgggcg ttgatctgac gcaccgaatc cgggctgcgg ccggcgtccg ccaacgccag
     1861 ctccatgcag gtgaccgcgc ccgacccgcc gggcgagggc gcggtgatgt gatgggcatc
     1921 cgccgtgctg cccgacccca ggacctcggc cagcggtgtg gcgccgcggg cgcgggcgtg
     1981 ctccagctcc tccagcacga ggacaccggc cccctcggcc atgacgaagc cgtcgcggtc
     2041 gcggtcgaag ggtcgggaca cccccgagct gctgagggcg gtcatgttct cgaagccggc
     2101 cactccggtc tgggtgatgg cgctctcggt gccgccggtg acgacggcgt tgcagcgacc
     2161 ggtcgcgatg agccgggcgg cgttgccgat ggagtgggtc cccgcggcgc aggccgtcac
     2221 cgtggtctca cagggtcccc ggaagccgtg gcgcatggag atgccggcgg cggcggcgtt
     2281 gggcatgagc atggggatga ggaacggtga gacccgttcg gggccccgga cccggttgac
     2341 ctcgacctgg tcctccaggg tgtggaggcc gccgatgccc gtgccgacca gcacgccgat
     2401 ccggtcgggg tccccgcccg ggtcgcccga ctgctcgagg gccaggccgg cggcccctgc
     2461 ggccagctgc gtgaaccggt cggcccgccg ggcgtccttg ggcccgtcga accagggtcc
     2521 cgggtcccag tcctggatct cccgggtctt gcccacctgg gccgggccga gcagggcggc
     2581 ccagaaggcc tcggccccga tgccgaccgc cgacacgacc ccgacgccgg tcacgaccac
     2641 ccgtcgtccg tccatcgtca gagcttggag cgaacgaggt cgacggcctg ggcgacggtc
     2701 gcgacgccct ccagctcctc ttcctcgatg ctgacgtcga actcgtcttc gagggccatg
     2761 accagctcga cgagatccag gctgtcggcg tcgaggtcgt cggcgaagtt ggcctcgagg
     2821 gtcaccttgt ccgcgtcgac ggcgagcacg gtgacggcat ggcccttgaa gcgctcgaac
     2881 agatcatcac tcacgggggt tcttctcctg tctcggtgaa ctcaaacgaa ctgggcctag
     2941 cttcccacgc ccatgcccag gccgccgtcg acgggcaggt tgacgccggt caggtaggcg
     3001 gcggcgtcgg aagccagata gcccacggcg gcggcgacct cttcgggctg gccgacccgg
     3061 ccgaggggca ccatcgacgt gaggagggcc ctctggtcgt cgtcgagggc ccgcaacatg
     3121 tcggtgtcga tgggcccggg gctgaccacg ttgacggtga tcgaccgcgg tgccagctca
     3181 cgggccaggg aacgggcgaa cccgaccagg ccggccttgc tggcggcgta gttggcctgg
     3241 ccggcggagc cgatctggcc cacgaccgag cccacgagga tgatccgccc ccagcgggcc
     3301 cgcagcatgc gcttggccgc ccgcttgctc acccggaacg caccggtgag gttggcgtcg
     3361 atcacccggc tgaagtcctc ctccttcatc atgagcagga gggtgtcggc gttgatgccg
     3421 gcggcgtgga cgaggacctc caccgggccg aacgcctcct cgacggcggc gaaggcggcc
     3481 tcgaccgtcg cggggtcggt gacgtcgcag ggcacgggga gcagccccgg ggcgtcgggg
     3541 gcctcgcccc gatgggtgat cgcgacgcgg tagcccgctt cggccagcgc cgtggcacag
     3601 gccagcccga ttccccggtt cccgcccgtc acgagggcga cgcgaccggc gtcggtcacc
     3661 ggccccaacg caggagtgtg ctggctccgg tcatcccggc gccgaagccc acgaggagca
     3721 ccagagcgcc gtcggccacg cgaccctggt cggccgcctc ggcgagcgcg tgcgggatcg
     3781 aggccgcgga cgtgtttccg gtgtgctcga gcaccgacac gactttctcg tcgggcacgc
     3841 caaggcgacg gcaggccgcg tcgatgatcc gctggttggc ctggtggggc acgagcagat
     3901 cgaggtcagc ggcctccacg cccgcccgtt ggagcgtcgc ctgcgcggag ctgacgatga
     3961 cccggaccgc ccgccggtag atctcgggcc cgtccatctt catggtgccg ccgacatcgg
     4021 cgtagagcag gttccggccg ctgccgtccg aactgaggtc ccaggccagg atccggtcct
     4081 cgtccacggc ctccagcacg acggcaccgc caccgtcacc gaaaaggatc gccgtgcccc
     4141 ggtcgtccca gtcggtgatg cgactcaggg tgtcggcccc gaccacgagc acgcaacggt
     4201 gcccgatcgc cagcaggccg tgggccacca cgaggccgta gacgaagccc gagcaggcgg
     4261 cattgaggtc gaaggccccg cagtccaggc cgaggatctc ttgcacggtc gaggcagtgg
     4321 ccggcaccgt ctggtccggt gtggtggtgg cgacgacgag ggcgtcgaca tcctccgggc
     4381 gccgcccg
//
LOCUS       DASTFV010000153         2880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_529202, whole genome shotgun sequence.
ACCESSION   DASTFV010000153 DASTFV010000000
VERSION     DASTFV010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529202"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VFP02_02885"
     CDS             complement(<1..386)
                     /locus_tag="VFP02_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET9541999.1"
                     /translation="MQPVEEIAERGYTILEGVFDAHRAEALVADLERLERALGTLPAG
                     NSFEGDRTLRVYNLLAHGALWEELAVDPAVLPVVEGVLDPGCLMSSLSSIRILPDETA
                     QPIHADDQVIPLDKPHAPTVCNTMWAL"
     gene            447..713
                     /locus_tag="VFP02_02890"
     CDS             447..713
                     /locus_tag="VFP02_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HET9542000.1"
                     /translation="MTPFEEAVAGVLAGLGVGDVVTYGEVAAEAGYPGAARAVGRFLA
                     THEGQPWWRVVTSTGRLVPQHTEEHARRLRAEGVRVEDGRVILR"
     gene            713..1201
                     /locus_tag="VFP02_02895"
     CDS             713..1201
                     /locus_tag="VFP02_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET9542001.1"
                     /translation="MAIYDHPIHSLDGSDADLSDYSDKVALLVNVASKCGLTPQYEGL
                     QKLQDTYGERGFTVLGFPCNQFLGQEPGSADEIADFCSMNYGVSFPLFEKIEVNGDER
                     HPLYDELTQVADAEGEAGDIQWNFEKFLVAPGGTSIQRFRPGVEPEAPELVAAIEADL
                     PG"
     gene            1204..1560
                     /locus_tag="VFP02_02900"
     CDS             1204..1560
                     /locus_tag="VFP02_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542002.1"
                     /translation="MAEARPEVDGARRRIGAYIVGVLAVLWVLVGVALVTIIGWEAIT
                     ADDRSCPVPGLDSVYGDSSWQAWPPGQVCTFGDARLEEPGAVRGAAIVFEIVIGVGLF
                     VVWRRYRHAPDPDWAA"
     gene            1724..>2880
                     /locus_tag="VFP02_02905"
     CDS             1724..>2880
                     /locus_tag="VFP02_02905"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9542003.1"
                     /translation="MSSTFESLGVAPDLVEALASRGITEPFPIQQLAIPPALEGRDVC
                     GKAKTGSGKTLAFGLPILGRVTKAEPRRPRALVLVPTRELALQVCEELRPLSIERNVK
                     VMAIYGGAPMEKQVAELEQGAEVVAATPGRLIDLIDRNECSVADIDIIVLDEADRMAD
                     MGFLPQVEWILRRIDNEHQTMLFSATLDGVVNGVIKRQCTDPAICEVEYAQVTVDEMH
                     HRFLHVHDMDKVRVTAAIAKGTERTLVFCNTKRAADRLERSLEKEGVDAAAIHGDLPQ
                     RVRERALKDFDEGRLHVLVATDVAARGIHVDDIDVVIHYDPPTDHKTYLHRSGRTARA
                     GESGIVVTLALWNQELEVKRLQTRIGVLDQPIVEVFSNDPRLGDLLAWDPS"
BASE COUNT          447 a         1011 c          986 g          436 t
ORIGIN      
        1 agggcccaca tcgtgttgca gacggtcggg gcgtggggct tgtcgagcgg gatcacctgg
       61 tcatcggcgt ggatgggctg tgccgtctcg tcgggcagga tccggatcga ggagagcgac
      121 gacatcaggc agccgggatc caggacgccc tcgacgacgg ggagcaccgc ggggtcgacg
      181 gccagctcct cccagagcgc gccgtgggcc aacaggttgt agacccggag ggtccggtcg
      241 ccctcgaagg agttgcccgc cggcaaggtg ccgagcgctc gctccagccg ctcgagatcg
      301 gccaccaacg cctcggcccg gtgggcgtcg aacacgccct ccaggatcgt gtagccccgt
      361 tcggcgatct cctccaccgg ttgcacgacg cgttcttacc cgcgatccgg tccgcctggg
      421 ggccacatcc cgggtaagaa cgggcgatga cgccgttcga ggaggcggtg gcgggcgtgt
      481 tggccgggct cggcgtgggc gatgtcgtca cgtacgggga ggtggcggcc gaggcgggct
      541 acccgggggc cgcccgggcc gtgggtcggt tcctggcgac gcacgagggc cagccctggt
      601 ggcgcgtggt caccagcacc ggtcgacttg tcccccagca caccgaagag cacgcccgcc
      661 gactgcgggc cgagggtgtc cgggtcgagg acggacgcgt aatactgcgt tgatggccat
      721 ctacgaccac ccgatccact cgctcgacgg cagcgacgcc gacctctccg actactcgga
      781 caaggtggca ctgctggtga acgtggcctc caagtgcggc ctcacgcccc agtacgaggg
      841 gttgcagaag ctgcaggaca cctacggcga gcggggcttc accgtcctcg gcttcccgtg
      901 caaccagttc ctcggccagg agccgggcag cgccgacgag atcgccgact tctgctcgat
      961 gaactacggc gtgtccttcc ccctgttcga gaagatcgag gtgaacggtg acgagcggca
     1021 cccgctctac gacgagctga cgcaggtggc cgacgccgag ggcgaggcgg gcgacatcca
     1081 gtggaacttc gagaagttcc tggtggcgcc cggggggacg agcatccagc gcttccgccc
     1141 gggtgtggag cccgaggccc ccgagctcgt cgccgccatc gaggccgacc tcccgggctg
     1201 agcgtggccg aggcccgccc cgaggtcgac ggggcccgac ggcggatcgg cgcgtacatc
     1261 gtcggcgtcc tggccgtgct ctgggttctc gtcggggtcg ctctggtgac catcatcggt
     1321 tgggaggcca tcaccgcaga tgaccggagc tgccccgtgc ccggcctcga ctccgtttac
     1381 ggcgattcgt cgtggcaggc ctggcccccg ggccaggtgt gcacgttcgg cgacgcccgg
     1441 ctggaggagc cgggggcggt ccggggcgcc gcgatcgtct tcgagatcgt gatcggtgtg
     1501 ggcctgttcg tggtctggcg gcgctatcgc catgccccgg atcccgactg ggccgcctga
     1561 cggtcctctc gttcggcgct catctcggta cgcttgacgg tcgaagggtc tgcatccccg
     1621 ccacgcaacc tcctggcgcc ggatggaccc tcccgggttg tcctctgtcc tgttgttcgc
     1681 cgagggcggc ccacccagga cacacgacaa gaaagacaac ccgatgagct ccacgttcga
     1741 gtcgctcggc gtggcccccg acctggtcga ggccctcgca tcccgcggca tcaccgagcc
     1801 cttcccgatc cagcaactcg ccatcccgcc cgccctcgag ggccgggacg tgtgtggcaa
     1861 ggccaagacc ggctccggca agaccctggc cttcggcctg cccatcctcg gccgggtgac
     1921 caaggccgag ccccgacggc cccgggccct ggtgctggtc cccacgcggg agttggccct
     1981 tcaggtctgc gaggagctca ggcccctctc gatcgagcgc aacgtgaagg tgatggccat
     2041 ctacggcggc gcccccatgg agaagcaggt ggcagaactg gagcagggcg ccgaggttgt
     2101 ggcggccacc cctggccgcc tcatcgacct catcgaccgc aacgagtgct ccgtcgccga
     2161 catcgacatc atcgtcctcg atgaggccga ccgaatggcc gacatggggt tcctgcccca
     2221 ggtcgagtgg atcctgcgcc gcatcgacaa cgagcatcag accatgctct tctcggccac
     2281 gctcgacggc gtggtcaacg gtgtcatcaa gcgccagtgc accgaccccg ccatctgcga
     2341 ggtcgagtac gcccaggtca ccgtggacga gatgcatcac cgcttcctgc acgtgcacga
     2401 catggacaag gtccgggtca ccgcggccat cgccaagggc accgagcgca cgctggtgtt
     2461 ctgcaacacc aagcgcgccg ccgaccgcct cgaacgcagc ctggagaagg aaggtgtcga
     2521 cgccgccgcc atccacggcg acctccccca gcgggtgcgg gagcgggcgc tcaaggactt
     2581 cgacgaagga aggctccatg tgctggtcgc caccgacgtg gcggcccgag gcatccacgt
     2641 cgacgacatc gacgtggtca tccactacga cccgccgacg gaccacaaga cctacctgca
     2701 ccgctccggc cgcacggccc gcgccggtga gtcgggcatc gtcgtcaccc ttgccctgtg
     2761 gaaccaggag ctcgaggtga agcggctcca gacccggatc ggtgtgttgg accagcccat
     2821 cgtcgaggta ttctccaacg atccccgact cggggacctg ctggcgtggg atccctccca
//
LOCUS       DASTFV010000154         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_531535, whole genome shotgun sequence.
ACCESSION   DASTFV010000154 DASTFV010000000
VERSION     DASTFV010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531535"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VFP02_02910"
     CDS             <1..725
                     /locus_tag="VFP02_02910"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HET9542004.1"
                     /translation="GEFDFVLGPERPAGYDGLFMEINGDAVHAITRDYLVDSRTGKRV
                     EWKIEASDRPATFRESDEDAARRFRAVITWLKDQAAMIPIPVGQQDDVDEEHNLGHRA
                     NPDSNQFDDPYPVPENNFGWSAGDAAYAFGKWDLAPDEALVIEGTSPECAFWSLNPWS
                     PYLHTFNYDYERLTLNHGQAVLDDDGSFTIVLAHEQPDHRNGLSLQGNRRGFLCFRWF
                     LPEKVPDPIRTKVIKVSEIAAL"
     gene            complement(729..1418)
                     /locus_tag="VFP02_02915"
     CDS             complement(729..1418)
                     /locus_tag="VFP02_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9542005.1"
                     /translation="MIDVVALDGDDTLWHSENQFVMTQDRFKELLAGRADPDLLDARL
                     MAHERENLQVFGYGVKGFTLSMIETAIEVTEGRIDTADIQAIIDGAKEMLQHPVELLD
                     GVAETVRALVKDYRVMVITKGDLFHQESKLARSGLVEDLWQVEIVAEKDEASYRRIFD
                     RYGLDPATFVMVGNSVKSDILPVLALGGRAVHIEYEMTWEMEHAEADKAHEGYWELDT
                     IRDLPTLLATL"
     gene            1441..>2261
                     /locus_tag="VFP02_02920"
     CDS             1441..>2261
                     /locus_tag="VFP02_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET9542006.1"
                     /translation="MLLCVGDLIEDVVVWLPGPVALGSDTVVRITRERGGSAANAAAA
                     AVRAGGRARFVGQLGDDELAHRLLHGLAMTGVDVVARHGGRTGSIVVLVDSTGERTML
                     TDRAAATELAAAEPEWLVGVDVLHLPAYSLFVERLAKSCLALAVMAQERGIPVTIDPS
                     SAALLEQYGVDRFKALLADVQPAILFPNADEAALLLSDDRPPEGVDLVVEKHGPDAVR
                     LITIEGVTEVPVPLVTGVVDTTGAGDAFAAGFLVAWTAGKPTVECVARGSETAAHV"
BASE COUNT          333 a          790 c          774 g          364 t
ORIGIN      
        1 acggcgagtt cgacttcgtc ctggggccgg agaggcccgc gggctacgac ggcctgttca
       61 tggagataaa cggcgacgcc gtccacgcca tcacccggga ctacctggtc gattcccgta
      121 ccgggaagcg ggtcgagtgg aagatcgagg cgtcggaccg tcccgccacc ttccgggagt
      181 ccgacgagga cgccgcccgc cgtttccggg ccgtcatcac ctggctgaag gaccaggcgg
      241 cgatgatccc catcccggtc ggccagcagg acgatgtcga cgaggagcac aacctcggcc
      301 accgggccaa cccggacagc aaccagttcg acgatccgta ccccgtgccc gagaacaact
      361 tcggttggag cgccggtgac gccgcctacg ccttcggcaa gtgggacctg gcccccgacg
      421 aggcgctggt catcgagggc acctcccccg agtgcgcgtt ctggagcctg aacccgtgga
      481 gcccctatct ccacacgttc aactacgact acgagcgcct gaccctcaac cacggccagg
      541 ccgtgctcga cgacgacggc tcgttcacga tcgtgctggc ccacgagcag cccgaccacc
      601 gcaacggcct ctctctgcag gggaaccgtc ggggctttct ctgcttccgc tggttcctgc
      661 ccgagaaggt ccccgacccc atccgcacca aggtcatcaa ggtcagtgag atcgccgctc
      721 tctgacgctc acagggtggc cagcagggtg gggaggtcgc ggatcgtgtc gagctcccag
      781 tagccctcgt gggccttgtc ggcctcggcg tgctccatct cccaggtcat ctcgtactcg
      841 atgtgcacgg cccgcccgcc gagggcgagc acggggagga tgtcggactt caccgagttg
      901 ccgaccatga cgaaggtggc cgggtcgagg ccgtagcggt cgaagatgcg gcggtagctg
      961 gcttcgtcct tctcggccac gatctcgacc tgccagaggt cctcgaccag gccggagcgg
     1021 gccagcttgc tctcctggtg gaacaggtcg cccttggtga tgaccatgac ccgatagtcc
     1081 ttgacgaggg cccgcaccgt ctcggccacc ccgtcgagga gctcgacggg gtgctggagc
     1141 atctccttgg ccccgtcgat gatggcctgg atgtcggcgg tgtcgatgcg gccctcggtc
     1201 acctcgatgg ccgtctcgat catggagagg gtgaagccct tcacgccgta gccgaagacc
     1261 tgcaggttct cccgctcgtg ggccatgagg cgggcgtcga gcaggtcggg atcggcgcgg
     1321 ccggccagga gctccttgaa ccggtcctgg gtcatgacga actggttctc gctgtgccag
     1381 agcgtgtcgt ctccatcgag ggccaccacg tcgatcacgc cccccttcta ccctgagccg
     1441 gtgctgttgt gtgtcggcga tctcatcgag gacgtggtcg tgtggctgcc cggtccggtc
     1501 gccctcggtt ccgacaccgt ggtgcgcatc acccgggagc gagggggcag tgcggcgaac
     1561 gcggcggcgg cggccgtgcg ggccggcgga cgggcgcgct tcgtcggcca gctgggtgac
     1621 gacgagctgg cccaccggct gctgcacggg ctggcgatga cgggggtcga cgtggtcgcc
     1681 cgccacggag ggcgcaccgg gtcgatcgtc gtgctggtcg actcgacggg tgagcgcacg
     1741 atgctgaccg atcgggccgc cgccaccgaa ctggccgccg ccgaaccgga gtggctcgtc
     1801 ggcgtcgacg tgctgcacct gcccgcttac tcactgttcg tcgaacgcct ggccaagagc
     1861 tgcctggcgc tggccgtcat ggcccaggag cgcgggatcc cggtgaccat cgacccctcg
     1921 tcggcggcct tgctcgagca gtacggcgtc gaccgcttca aggccctgct ggccgacgtg
     1981 cagcccgcca tcctgttccc caacgccgac gaggccgctc tgctcctgag cgacgacagg
     2041 ccgccggagg gcgtcgacct ggtcgtcgag aagcacggcc ccgacgccgt ccgcctgatc
     2101 accatcgagg gcgtgactga ggtgccggta cccctggtga ccggggtggt cgacaccacc
     2161 ggcgccggtg acgccttcgc cgccggcttc ttggtcgcct ggacagccgg gaagcccacc
     2221 gtcgagtgcg tggcccgcgg cagcgagacc gcggcccacg t
//
LOCUS       DASTFV010000155         4569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_532058, whole genome shotgun sequence.
ACCESSION   DASTFV010000155 DASTFV010000000
VERSION     DASTFV010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532058"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFP02_02925"
     CDS             <1..240
                     /locus_tag="VFP02_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HET9542007.1"
                     /translation="MALDPQLLEILACPEDKGPLLYFEDENLLYNPRLQRKYRIDDDI
                     PVMLVDEAEAADDAEHERLLAKADATGIEPTFSDG"
     gene            233..1264
                     /locus_tag="VFP02_02930"
     CDS             233..1264
                     /locus_tag="VFP02_02930"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HET9542008.1"
                     /translation="MADRVDSLEMFATAAAFPEQIDEAVATAHQVEGLPDGTQIDNVL
                     VLGMGGSGVAGDLLAHIAGPFMPVPVVVVKGYEPPSYVNERSLVFAMSFSGHTEETLE
                     AVTTAANAGGRIVAVTRGGALAELCRSWGAPVVGIADDIPQPRAGLGALAAPMMIVLE
                     RLGLYPGAEGWLRFAVDQLKARRDKLVLDGNPAERLADDLARRVPVIYGGGGLGGLAA
                     TRWKTEFNENSRSPAFANTVPELCHNEIAGWGQNGDLTRQVFALINLRHDFEHPQIAR
                     RFDVMDPWLDEVVGDIFEVRAAGEGSLAQLLDLCMMGTFTSLHLAVREGIDPGPVPVL
                     DQLKAALGE"
     gene            complement(1278..1634)
                     /locus_tag="VFP02_02935"
     CDS             complement(1278..1634)
                     /locus_tag="VFP02_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9542009.1"
                     /translation="MPVVRIDHVQLAMPVGREDEATAFYEGLLGLPRVPKPDPLAARG
                     GCWFEGDEVKVHLGVEADFRPARKAHPALVVDDLSALVERLRDVGVEVREGDDPSGRP
                     QVYVDDPFGNRIELVG"
     gene            1738..3213
                     /gene="ahcY"
                     /locus_tag="VFP02_02940"
     CDS             1738..3213
                     /gene="ahcY"
                     /locus_tag="VFP02_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET9542010.1"
                     /translation="MSTNTDARPTTVADGDYKVADLSLAAFGRKEIQLAEHEMPGLMA
                     LRAEYYDSQPLAGARIMGSLHMTIQTAVLIETLTTLGAQVRWVSCNIFSTQDHAAAAV
                     VVGPEGTVDDPKGVPVYAWKGETLEEYWWCTEQALVWPDDGEGPNMILDDGGDATMLV
                     HLGTEYERAGEVPDPSTAESEEFEVVLQTLTRTLGDDPKLWHRIGERIKGVTEETTTG
                     VHRLYERHAAGTLLFPAINVNDSVTKSKFDNLYGCRHSLIDGINRATDVMIGGKVAVV
                     CGYGDVGKGCAQSLRGQGARVIITEVDPICALQAAMEGFQVTTLEDVIETADIFVTAT
                     GNKDIISAEHMARMKHQAIVGNIGHFDNEIDIAGINKMSDVQKVEVKPQVHEYVFPDG
                     HSVIMLAEGRLLNLGCATGHPSFVMSSSFTNQVIAQIELFAHTDAYELGVHVLPKHLD
                     EKVARLHLDALGVKLTKLTEDQAAYIGVKPSGPYKSTHYRY"
     gene            3218..4039
                     /locus_tag="VFP02_02945"
     CDS             3218..4039
                     /locus_tag="VFP02_02945"
                     /inference="COORDINATES: protein motif:HMM:NF004843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9542011.1"
                     /translation="MDDLEGKVAVVTGAASGIGRALADRWVTEGMKVVLADVEEGPLD
                     AARDELDASGADVLAVRTDVSDAAQVEALAAATLDHFGAVHLVCNNAGVGGGGLIWEA
                     ELEEWDWVLGVNLLGVVHGIKAFVPHLVAQDEGHVVNTASIAGFAYGPMMGPYNASKA
                     AVVAISETLKADLALQGSKVGVSVLCPGWVNTRIHESDRNRPGGPLADDEITAGRQML
                     GEVLKGGKQPDEVAHLVAAAVHEGTTHIFTGDEWIGLARGRLETVLTELPRDDGS"
     gene            4132..>4569
                     /locus_tag="VFP02_02950"
     CDS             4132..>4569
                     /locus_tag="VFP02_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542012.1"
                     /translation="MVEATTVPDLCALITTLAVRGAPALGAAGAMGVALAHVRGEDTA
                     AAADVLIATRPTAANLAWGARRALAAEDPVGEAVTIATDDVECCRAIGRNGAALVPED
                     SRILTHCNAGGLACVGYGTAVGVIRAAHEAGRRPRVWVDETRPV"
BASE COUNT          730 a         1657 c         1544 g          638 t
ORIGIN      
        1 atggcgctcg acccgcagct gctcgagatc ctggcgtgcc ccgaggacaa gggcccgctg
       61 ctgtacttcg aggacgagaa cctgctctac aacccccgtc tccaacggaa gtaccgcatc
      121 gacgacgaca tcccggtcat gctcgtggac gaggccgagg cggctgatga tgccgagcac
      181 gagcgcctcc tggccaaggc cgacgccacc ggcatcgagc cgacgttcag cgatggctga
      241 tcgagtcgac tcgctcgaga tgttcgccac ggcggcggcg ttcccagagc agatcgacga
      301 ggccgtggcc acggcccacc aggtcgaggg actgcccgac ggcacccaga tcgacaacgt
      361 gctcgtcctc ggcatgggtg gcagcggcgt ggccggcgac ctgctggccc acatcgccgg
      421 gccgttcatg ccggtgcccg tcgtcgtggt caagggttac gagccaccgt cgtacgtcaa
      481 cgagcgcagc ctggttttcg ccatgtcgtt ctcgggccac accgaggaga ccctcgaggc
      541 ggtgacgacg gcggccaacg cgggtggtcg catcgtggcc gtcacccggg gtggcgcgct
      601 ggccgagctc tgccggtcgt ggggtgcgcc cgtcgtcggc atcgccgacg acatccccca
      661 gccgcgggcc ggcctggggg cgttggcggc gccgatgatg atcgtcctgg agcgcctcgg
      721 tctctacccc ggcgccgagg gttggctgcg cttcgccgtc gaccagctca aggcccgccg
      781 ggacaagctc gtcctcgacg gcaacccggc cgagcgcctg gccgacgacc tggcccgtcg
      841 ggtccccgtc atctacggcg ggggtggcct cgggggcctg gccgccacgc gttggaagac
      901 cgagttcaac gagaacagcc ggtcgcccgc cttcgccaac accgtccccg agctctgtca
      961 caacgagatc gcgggctggg gccagaacgg cgacctgacc cgccaggtct tcgccctgat
     1021 caacctgcgc cacgacttcg agcacccgca gatcgcccga cgcttcgacg tcatggaccc
     1081 ctggctggac gaggtggtgg gcgacatctt cgaggtccgg gccgcgggtg agggcagcct
     1141 ggcccagctc ctggacctct gcatgatggg caccttcacc agcctccacc tcgccgtccg
     1201 cgagggcatc gaccccggcc ccgtccccgt cctggaccag ctcaaggccg cattggggga
     1261 gtagaaggca gcccggatca gccgacgagc tcgatgcggt tgccgaaggg atcgtcgacg
     1321 tagacctgcg ggcggccgga cgggtcgtcg ccctcccgga cctcgacccc gacgtcccgg
     1381 agccgctcga ccagggcact gaggtcgtcc acgaccagtg ccgggtgggc cttgcgggcg
     1441 ggccggaagt cggcctcgac gccgaggtgg accttcacct cgtcgccttc gaaccagcag
     1501 ccaccccggg cggcgagcgg gtcgggcttt ggcacccggg gcaggccgag gaggccctcg
     1561 tagaacgccg tggcctcgtc ctcacgcccc accggcatcg ccaactggac gtggtcgatc
     1621 ctcaccaccg gcaccggggc agcatgccac taccctgtcc gcggggctga tcgagaggcg
     1681 tcagtggacc ccagccccgt atgcgaaacc ccaagctcct gcccgaaagg caccacgatg
     1741 tccacgaaca ctgacgcgcg tccgaccacc gtcgccgacg gcgactacaa ggtcgccgat
     1801 ctctccctgg ccgccttcgg ccgcaaggag atccagctgg ccgagcacga gatgcccggg
     1861 ctgatggccc tgcgggccga gtactacgac agccagccgc tggcgggggc ccggatcatg
     1921 ggctcgctgc acatgaccat ccagacggcc gtgctgatcg agaccctgac caccctcggc
     1981 gcccaggtcc gctgggtgtc ctgcaacatc ttctccaccc aggaccatgc cgccgccgcg
     2041 gtcgtcgtcg gccccgaagg caccgtcgac gaccccaagg gtgtcccggt ctacgcctgg
     2101 aagggcgaga cgttggagga gtactggtgg tgcaccgagc aggccctggt gtggcccgac
     2161 gacggtgaag gcccgaacat gatcctggac gacggcggcg acgccacgat gctcgtccac
     2221 ctcggcaccg agtacgagcg ggccggcgag gtcccggacc cctccacggc cgagagcgag
     2281 gagttcgagg tcgtgctcca gaccctcacc cggacgctgg gcgacgatcc caagctctgg
     2341 caccgcatcg gtgagcgcat caagggcgtc accgaggaga ccacgaccgg cgtgcaccgc
     2401 ctctatgagc gccacgccgc gggcacgctg ctcttcccgg cgatcaacgt caacgactcg
     2461 gtcaccaagt ccaagttcga caacctctac ggctgccgcc acagcctcat cgacggcatc
     2521 aaccgggcca ccgacgtcat gatcggcggc aaggtcgccg tggtctgcgg ctacggcgac
     2581 gtcggcaagg gctgtgccca gtcactccgg ggccagggcg cccgggtcat catcaccgag
     2641 gtcgatccca tctgtgccct ccaggcggcg atggagggct tccaggtcac gacgctggag
     2701 gacgtcatcg agacggccga catcttcgtc accgccaccg gcaacaagga catcatctcc
     2761 gccgagcaca tggcccggat gaagcaccag gccatcgtcg gcaacatcgg ccatttcgac
     2821 aacgagatcg acatcgccgg catcaacaag atgtccgacg tccagaaggt cgaggtcaag
     2881 ccccaggtcc acgagtacgt gttcccggac gggcactcgg tgatcatgct ggccgagggt
     2941 cggctgctga acctgggttg tgccaccggc caccccagct tcgtgatgtc cagcagcttc
     3001 accaaccagg tcatcgccca gatcgagttg ttcgcccaca ccgacgccta cgagctcggc
     3061 gtgcacgtgc tgcccaagca cctggacgag aaggtggccc gcctccacct cgacgctctg
     3121 ggcgtgaagc tgaccaagct caccgaggac caggccgcgt acatcggcgt caagccgagc
     3181 ggcccctaca agtccacgca ctaccggtac tgagcccgtg gacgacctcg aaggaaaggt
     3241 cgcggtcgta acgggcgcgg ccagcggcat cggccgggcg ctggccgacc gctgggtcac
     3301 cgagggcatg aaggtcgtcc tggccgacgt cgaggaggga cccctcgacg cggcccggga
     3361 cgagctcgac gcgagcgggg ccgatgtcct cgccgtccgg accgacgtgt ccgatgcggc
     3421 gcaggtcgag gccctggccg cggccaccct cgaccacttc ggtgccgtgc acctcgtgtg
     3481 caacaacgcc ggcgtgggcg gcggtggcct catctgggag gccgagctcg aggagtggga
     3541 ctgggtgctg ggcgtcaacc tcctcggggt cgtccacggc atcaaggcct tcgtccccca
     3601 cctcgtggcc caggacgagg gtcacgtggt gaacacggcg tccatcgccg ggttcgccta
     3661 cggcccgatg atgggcccct acaacgcctc gaaggccgcc gtcgtggcca tctccgagac
     3721 cctgaaggcc gatctggccc tgcagggctc gaaggtcggc gtgagcgtgc tctgcccggg
     3781 ctgggtcaac acccgcatcc acgagtcgga ccgcaaccgc cccggagggc ccttggccga
     3841 cgacgagatc acggcgggtc gccagatgct gggcgaggtc ctgaagggcg gcaagcagcc
     3901 cgacgaggtc gcgcacctcg tggcggccgc cgtgcacgag gggaccaccc acatcttcac
     3961 cggtgacgag tggatcggcc tggcccgagg ccggctcgag accgtgctca ccgagctgcc
     4021 gcgggacgac gggtcctgac cgacctatcc gatccgatcc caccgaccat cgagtgggcg
     4081 ggcgatcggg tccgcctcat cgaccaacgc ctcctgcccg gtcgactcga ggtggtcgag
     4141 gccacgaccg ttcccgacct gtgtgccctg atcacgaccc tggccgtgcg gggcgccccg
     4201 gcgctcggcg cggccggggc gatgggtgtg gcgctggccc atgtgcgggg cgaggacacc
     4261 gctgccgccg ccgacgtgct catcgccacc cggccaacgg ccgcgaacct ggcctggggc
     4321 gcccgccggg ccctggccgc cgaggatccg gtcggggagg ccgtgaccat cgccaccgac
     4381 gatgtcgaat gctgtcgcgc catcggccgg aacggtgccg ccctggttcc cgaggacagc
     4441 cgcatcttga cccattgcaa cgccgggggc ctggcctgcg tgggctacgg caccgcggtc
     4501 ggcgtcatcc gcgccgccca cgaggccggg cgtcgccctc gggtctgggt cgacgagacc
     4561 cgcccggtg
//
LOCUS       DASTFV010000156         5123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_533038, whole genome shotgun sequence.
ACCESSION   DASTFV010000156 DASTFV010000000
VERSION     DASTFV010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533038"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VFP02_02955"
     CDS             <1..415
                     /locus_tag="VFP02_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HET9542013.1"
                     /translation="LADAVPQWADLAANPPAVFIGGPVEQGAVLGLGRLVAEVPPEGL
                     TPVHAGIGVLDLEADPVRLVGDVGGVRLFTGYSGWGPGQLEDELAMGGWFVVDAEPDD
                     VTTTEPDDLWRAVLRRQPDDDLARLALFPNDLSAN"
     gene            418..1209
                     /locus_tag="VFP02_02960"
     CDS             418..1209
                     /locus_tag="VFP02_02960"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9542014.1"
                     /translation="MTTAPLDGVRIEYELAGTPGQRRLLSISGSGSDLRRPPRTQDEA
                     VLAERFEILAYDHRGLGRSVPDDPHREPTMADYAADAVGLLDHVGWDTCRVLGISFGG
                     MVAQELAVTAPVRIERLVLACTSPGGAGGSSSPLHTLLDMADDERLAQQVAWLDDRTV
                     DDPELRAFWTEMMAGLAGGEPTEGERHQLQARSHHDVWDRLPQLALPVLIAAGRFDGI
                     ALPANQEALARQIEGADLRWFDGGHAFFLQDRTALPAIADWLAEG"
     gene            complement(1300..3435)
                     /locus_tag="VFP02_02965"
                     /pseudo
     CDS             complement(1300..3435)
                     /locus_tag="VFP02_02965"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
     gene            3450..4052
                     /locus_tag="VFP02_02970"
     CDS             3450..4052
                     /locus_tag="VFP02_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9542015.1"
                     /translation="MKAVTRERNPRGQGERLRVDLIDAAVDLIAETGSVDGVSLRAAA
                     KRAGVSPMAVYNHFADKDALLVAAVEHCWDEFQAAIAVTLDEPEPSARLRAAGDAYVR
                     FALEHPGRYAVMFSGTRNLPERAVPIGMSAFDTLVAMVADILDARHDGRDPAFVAVQV
                     HTWIHGMVSLLGCAPDGPWPSTDTLLDDLMVRLGLGPPTA"
     gene            complement(4067..4531)
                     /locus_tag="VFP02_02975"
     CDS             complement(4067..4531)
                     /locus_tag="VFP02_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET9542016.1"
                     /translation="MTELRFDDVEAINAAAGGDYGDWGREIEITQEMINQFAELTGDH
                     QWIHVDIERANAGPFGGPIAHGFLTLSLLPALSGGTDLAVTGHANAVNYGADGLRFLA
                     PVPAGSKVHARSKLVSAEAKGSGTLVKTETAVHVVDVEKPSLIYQMLVLYMG"
     gene            complement(4606..>5123)
                     /locus_tag="VFP02_02980"
     CDS             complement(4606..>5123)
                     /locus_tag="VFP02_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542017.1"
                     /translation="WCRATNADLDVAAALDGHVLPDETGRAGAALLEAGEVYRRTGVV
                     QPNGSALHDALVGGGTGGVGAPTEATIAEVVATLDRADDELAAAVFTDPDRAITRREL
                     RQAVALARHGAWRLARIHELATPDNEALASDLAHLHEEQAACWDARSRPGGREDSLGR
                     LPSPSGSTPGP"
BASE COUNT          771 a         1821 c         1857 g          674 t
ORIGIN      
        1 cctggccgac gctgtgcccc agtgggccga tctggcggcc aacccgcccg cggtcttcat
       61 cggaggcccg gtcgagcagg gcgccgtgct cggcctcggg cgcctcgtcg ccgaggtccc
      121 cccggagggg ctgaccccgg tccacgccgg catcggcgtg ctcgacctcg aggccgatcc
      181 cgtccgtctc gtgggcgatg tcggcggcgt ccgcctcttc accggctact cgggttgggg
      241 ccccggccag ttggaggacg agctggccat gggcggctgg ttcgtcgtgg acgccgagcc
      301 ggacgatgtg acgaccaccg agcccgacga cctctggcgg gccgtcctcc gccgccagcc
      361 cgacgacgac ctcgcccgcc tcgccctctt ccccaacgac ctctccgcca actgaaggtg
      421 acgacggcgc cgctggacgg ggtgaggatc gagtacgagc tggccgggac gccagggcag
      481 cgccggctcc tgtccatctc gggctccggc agcgacctcc gacgcccgcc ccggacccag
      541 gacgaggcgg tgctggccga gcgcttcgag atcctggcct acgaccaccg gggactcggc
      601 cgctccgtgc ccgacgaccc gcaccgggag ccgacgatgg ccgactacgc ggcggacgcc
      661 gtcggccttc tcgaccacgt gggctgggac acctgccggg tcctcgggat ctccttcggc
      721 ggcatggtcg cccaggagct ggccgtcacc gcgccggtgc gcatcgagcg cctggtgctg
      781 gcgtgcacgt cgccaggcgg agcaggcggg tcgtcctcgc cgctccacac gctgctggac
      841 atggccgacg acgagcgcct ggcgcagcag gtggcttggc tcgacgaccg cacggtcgac
      901 gaccccgagc tgcgggcctt ctggaccgag atgatggccg gccttgccgg gggcgagccg
      961 accgagggcg agcgccacca gttgcaggcc cggtcccatc acgatgtgtg ggaccggctc
     1021 ccgcagctgg cgctacccgt cctgatcgcc gcggggcgct tcgacggcat cgccctgccc
     1081 gccaaccagg aggcgctcgc ccgtcagatc gagggcgcgg atctccgctg gttcgacggt
     1141 gggcacgcgt tcttcctcca ggaccgcacc gcgctgcccg ccatcgccga ctggctggcc
     1201 gagggctgag gccgcggcgg cgcggtcgac ctcgtcgggc gcggcctcgt ggaggccgaa
     1261 gcgctgctgg aggcgctcga gcggccccgg ggcccaccag ttggcgtcac cggccagctt
     1321 catgaacgcc ggcaccagca gggcccgcac gatggtggcg tccatcacca cggccatggc
     1381 gaggccaaga ccgaagagct tgatgaaggt gatgcccgag gtggcgaagg ccaggaacgt
     1441 gacggccagc agcaaggcgg cggcggtgac gatgcggccg gtgtgctcca ggcccgccgc
     1501 cacggcgtgc tcgttgtcgc cggtccggtc gtactcctcc ttgatgcggg agaggaggaa
     1561 gacctcgtag tccatcgaca gcccgaaggc gatgcagaac atgaggatcg gcgtcgtcgt
     1621 gtccgtcagc ccggtcggcg tgaagtccag caggccggca ccatggccct cctggaagat
     1681 ccagaccatg gccccgaagg tggcggtcag gctcagcgtg ttgaggacca gggccttcac
     1741 cggcaccagg atcgacccga acatggcgaa cagcaggatg aacgtggcta ccgcgatcca
     1801 cagggccgcc cagggcacga ggccgaagat ggcgtccttc acgtcgatct gctgggcgcc
     1861 catcccgccg accaggacct cgtcgaaggg ggcgtcgaga tcccggacgg actcgaccaa
     1921 ggcctcgccc tcctccgaga tgggttcgac ggtgggcacg aggctgaacc acgtcgcccc
     1981 gtcggcccgg aactgctcgg tggtggcgtc ggggccgatg agcagcgtgc cgtcggcgta
     2041 acgaccggtg cgggcgtcga cccgggcgat gccgggcagg gccgacagct cgctggcgta
     2101 ggcctcgacg tcggtgtcgt ccaccgtccc gagggcgacc accgggaagg cgtcggcctc
     2161 gtcggacacg aagtcgtccc gcagggcctc ggacacctcg cgggcgggat tgccctcggg
     2221 cagcacgcgg tgatccggtg agccgaactg cgcctgcagg aagggaaggc cgagtccgac
     2281 cagcaacgcc accacggcga cggtcaccac gaacggccgg cgcatgacgg cggtggccag
     2341 gcggtgccag aaccccgtgc cgacggcggg cgtctcccgg tgacggaaga cccgccagcg
     2401 atcgacccgc gggccgagca cggccagcag ggcggggagg ctgaccagcg aggccagcat
     2461 ggcgaccagg atcaccccga tgccggcgta ggcgaacgag cgcaggaaga acagcgggaa
     2521 gatgagcagg gcggagaggg acgcggccac ggtgagggcg ctgaaggcga ccgtgcgccc
     2581 cgcggtctcg acggtgcgca cgacggcgtc gtcgacgctg cgcccggcgt gcagctcttc
     2641 tcggaaccgg gacaccacga acaggctgta gtcgatggcc aggccgagcc ccatggccgt
     2701 caccaggttg atggagaaca ccgagacgtc ggtcaccgcg gtgaccagcc agaggacgaa
     2761 gaaggcgccg agcaccgcca gcatcccgac cccgacaggg agaagggcgg ccaccacgcc
     2821 cccgaacacg atgatgagca ggatcagggt gatgggcacg gccacgctct cagcccgggc
     2881 cagatcgccc tcgatggtct caccgatctg gttgaagatc tcgaccgagc ccccgacctc
     2941 gacggtcacc ggaccgcgct caccgctgaa ggtgtcggcc aagccgtcgg cggcgtcctg
     3001 ccactcgtcc tggtcgccgg ccaaccggcc cacgaccatg gcggccgagc cgtcctcgct
     3061 ccgcagcggt ggcggcgagc cgagcgacca gtacgacacg acgtcgtcgg ccttggggta
     3121 ggcggccagt tcgtcggtca gctgctggcc gacggccgcc acctcgggtg cgtcgacatc
     3181 gccgtcgccg gcggtgacca ggaggacgac gttgggtgcc ccggcgccga agcgggcctc
     3241 gagcaggtcg tcggcccggg tcgactccga atccgggtcg gcgaagccgc cgctggacag
     3301 ccggtcgaac accgcgccgc cgagcacgcc ggcaatgatg acgacgacga cggtcagcac
     3361 cagcacgagg cgccggcgac ggacggtgaa ctggccgatg cgggtgaaca cggttgtgtg
     3421 tttacgctcg tacatctacg ctcgtcaacg tgaaagcggt cacacgggag cgcaaccccc
     3481 gaggccaagg cgaacgcctc cgggtcgacc tgatcgatgc cgccgtcgac ctgatcgccg
     3541 agaccggttc ggtcgacggg gtcagcctgc gggccgcggc caaacgggcc ggcgtctcgc
     3601 cgatggcggt ctacaaccac ttcgcggaca aggacgccct gctggtggcc gccgtcgagc
     3661 actgctggga cgagttccag gcggccatcg ccgtcaccct cgacgaaccc gagccgtcgg
     3721 cccggttacg ggcggcgggg gacgcctacg tccgcttcgc cctcgagcat cccggccggt
     3781 acgcggtcat gttctccggc acccgcaacc tgcccgagcg ggccgtgccc atcggcatga
     3841 gtgccttcga cacgctggtg gccatggtgg ccgacatcct cgacgcccgc cacgacggac
     3901 gggaccccgc gttcgtcgcc gtccaggtcc acacctggat ccacggcatg gtcagcctgt
     3961 tgggatgcgc gcccgacggc ccctggccgt cgaccgacac cctgttggac gatctcatgg
     4021 tccgcctcgg cctcggcccc ccgaccgcct gacgcgaccc ggatggtcag cccatgtaga
     4081 gcacgagcat ctggtagatg aggctgggct tctcgacgtc gaccacgtgg acggcggtct
     4141 cggtcttgac gagcgtgccg ctgcctttgg cctcggcgct gaccagcttg ctgcgggcgt
     4201 ggaccttgct gccggcgggg accggagcca ggaagcgcag cccgtcagcg ccgtagttga
     4261 cggcgttggc atggccggtg acggcgaggt cggtcccgcc ggagagggcc gggagcagcg
     4321 acagggtcag gaagccgtgg gcgatcggcc caccgaaggg gccggcgttg gcccgctcga
     4381 tgtccacgtg gatccactgg tggtcgccgg tcagctcggc gaactggttg atcatctcct
     4441 gggtgatctc gatctcccgg ccccagtccc cgtagtcgcc acccgcagcg gcgttgatgg
     4501 cttcgacgtc gtcaaatcgc agttcggtca cgggagagaa cgtagatcag gcggaccgac
     4561 cgacccgcac cgaccggcgg gacgacggct cccgaggagc aaccgtcagg gtcccggcgt
     4621 cgaccccgaa gggctcggga gccggcccag gctgtcctcc cggccccctg ggcgactgcg
     4681 ggcgtcccag caggccgcct gctcctcgtg caggtgggcg agatcggagg ccagggcctc
     4741 gttgtccggc gtagcgagct cgtggatgcg ggccaggcgc cacgcaccgt ggcgggccag
     4801 ggccacagcc tgccgcagct cgcgccgggt gatcgcccgg tcagggtcgg tgaagacggc
     4861 ggcggcgagc tcgtcgtcgg cccggtccag cgtggcgacg acttcggcga tggtggcctc
     4921 ggtgggggcg cctacgccac ccgtgccgcc gcccaccagc gcatcgtgga gggccgagcc
     4981 gttgggctgg acgacgccgg tgcggcggta gacctcgccc gcttccagca gcgcggcccc
     5041 ggctcgaccg gtttcgtcgg gcagcacgtg accgtccagg gccgcagcca cgtccaaatc
     5101 ggcgttggtc gcccggcacc agg
//
LOCUS       DASTFV010000157         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_536830, whole genome shotgun sequence.
ACCESSION   DASTFV010000157 DASTFV010000000
VERSION     DASTFV010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536830"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VFP02_02985"
     CDS             <1..502
                     /locus_tag="VFP02_02985"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET9542018.1"
                     /translation="EPGTRTGVAIRTDNPKEVGADRIANAVAAFDLYGGPTIMVDFGT
                     ATTCDAVSADGEYLGGAIAPGIEISLDALVGRAAALRQVEIAEPRSVLGRTTVESIQS
                     GIVYGYAAQVDGLCARIEEELGECTVVGTGGLAPLIAPFAESVQHIEPWLTLHGLRLV
                     HAMNVE"
     gene            499..1923
                     /gene="lysS"
                     /locus_tag="VFP02_02990"
     CDS             499..1923
                     /gene="lysS"
                     /locus_tag="VFP02_02990"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET9542019.1"
                     /translation="MSDGPGPRIPYRVEPTHHAAQIVDQYAGIADGEETGVDVTVAGR
                     LMLQRPHGRLAFAVLRDMTGDVQLFSLEKVTAGYADLIALPLGTWVSATGEVVKTRKG
                     ELSVKVATWEVLARPQRPFPDKWHGLNDPDTRYRQRYVDTWVTPEARSTLLARSQLVS
                     LCRRWLEERGFIEVETPIFHPIPGGALAKPFRTHHNALDVELFLRIAPELYLKRLVVA
                     GFERVFEIGRVFRNEGLSPRHNPEFTMLELYQAYADYGDIMALTEELVAYCATEITGG
                     TTVMVGDRELDLSAPWPRASLTDLVEQAIGVRVEVRMPVEDVRAIALEHGLEPEPSWG
                     SGKILLELYEKHVEVTLWDPVFVMDYPKEVSPLSRDHRELPEMVERFEAIVAGRELCN
                     AFSELIDPVEQRARFEDQVRQGEAGDDEAMAVDEDYLRALEYGLPPTGGLGIGIDRLV
                     MLLTGSSAIRDVIAFPTLRPEQES"
     gene            1926..>2169
                     /locus_tag="VFP02_02995"
     CDS             1926..>2169
                     /locus_tag="VFP02_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9542020.1"
                     /translation="MRVLLTGMGGDFGARTANVVEADPAVEAVLGLDAYPPRRRLHGS
                     EFRRIDPRDESRTTAAVRDFAPTVVLHLGVWEPDARA"
BASE COUNT          310 a          810 c          737 g          312 t
ORIGIN      
        1 cgagccgggg acccggacgg gggtggccat ccgcacggac aaccccaagg aggtcggggc
       61 ggaccgcatc gccaacgccg tcgccgcctt cgacctctac ggcgggccca cgatcatggt
      121 cgacttcggc acggccacga cgtgcgacgc ggtgtcggct gacggtgagt acctcggcgg
      181 cgccatcgcc cccgggatcg agatcagcct cgacgccctc gtcgggcggg ccgccgccct
      241 gcgtcaggtc gagatcgcgg agccccgttc ggtgctgggt cggaccacgg tcgagtcgat
      301 ccagtcgggc atcgtctacg ggtacgccgc ccaggtcgac gggctctgtg cccgcatcga
      361 ggaggagctg ggggagtgca ccgtggtggg cacgggcggc ttggccccgc tcatcgcccc
      421 ctttgccgag agcgtgcagc acatcgagcc ctggctcacc ctgcacggcc tccgcctcgt
      481 ccacgccatg aacgtcgaat gagcgacggc cccgggcccc gcatccccta ccgggtcgag
      541 cccacccatc acgccgccca gatcgtcgac cagtacgccg gcatcgccga cggcgaggag
      601 accggcgtcg acgtgaccgt cgccgggcgc ctcatgctcc agcggcccca cgggcgcctg
      661 gccttcgccg tgctgaggga catgaccggc gacgtccagc tcttctcgct ggagaaggtg
      721 acggccggct acgccgacct catcgccctg cccctcggca cctgggtcag cgccaccggc
      781 gaggtggtca agacccgcaa gggcgagctg tcggtgaagg tggccacgtg ggaggtgctg
      841 gcccggccgc agcgaccctt tcccgacaag tggcacggcc tcaacgaccc cgacacgcgc
      901 taccggcagc gctatgtcga cacctgggtc acgccggagg cccgctcgac cctgctggcc
      961 cgctcccagc tcgtctcgct gtgccggcgc tggttggagg agcgcggctt catcgaggtc
     1021 gagaccccga tcttccaccc catccccggc ggggcgctgg ccaagccctt caggacccac
     1081 cacaacgcgc tggacgtcga gctcttcctg cggatcgccc ccgagctgta tctgaagcgc
     1141 ctggtcgtgg ccggcttcga acgcgtcttc gagatcggcc gggtcttccg caacgagggc
     1201 ctctcccccc gccacaaccc cgagttcacg atgctcgagc tctaccaggc ttacgccgac
     1261 tacggcgaca tcatggccct caccgaggaa ctggtggcct actgcgccac cgagatcacc
     1321 ggcgggacga cggtcatggt cggcgacagg gagctcgacc tgtccgcgcc ctggccccgg
     1381 gcctcactga ccgacctggt cgagcaggcc atcggcgtcc gggtcgaggt ccggatgccg
     1441 gtcgaggacg tgcgggccat cgccctcgag cacggcctgg agcccgagcc gtcctggggc
     1501 tcgggcaaga tcctgctcga gctgtacgag aagcacgtcg aggtcaccct ctgggacccc
     1561 gtcttcgtca tggactaccc gaaggaggtg tccccgttgt cgcgggacca ccgggagctg
     1621 ccggagatgg tcgagcgctt cgaggccatc gtggccggcc gcgagctctg caacgccttc
     1681 tccgagctca tcgacccggt cgagcagcgg gcccgcttcg aggaccaggt ccgccagggc
     1741 gaggccggcg acgacgaggc catggccgtc gacgaggact acctgcgggc gctggagtac
     1801 gggctgccgc cgaccggggg gttgggcatc ggcatcgacc gtctggtcat gttgctgacc
     1861 ggctcgtccg ccattcgcga cgtgatcgcc ttcccgaccc tccgacccga acaggagagc
     1921 tgacggtgcg tgtccttctg accggcatgg ggggtgactt cggcgcccgc accgccaacg
     1981 tggtcgaggc cgaccccgcg gtagaggccg tgctcgggct ggacgcctac ccgcctcggc
     2041 gccggctgca cggcagcgag ttccgccgca tcgacccccg ggacgagtcg cgcaccaccg
     2101 ccgcggtccg agacttcgcc cccaccgtcg tgctccacct cggcgtctgg gagcccgacg
     2161 cccgggccg
//
LOCUS       DASTFV010000158         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_544814, whole genome shotgun sequence.
ACCESSION   DASTFV010000158 DASTFV010000000
VERSION     DASTFV010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544814"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFP02_03000"
     CDS             <1..348
                     /locus_tag="VFP02_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542021.1"
                     /translation="PLWIGGRSYRSLRRAVELADGWAPFGVTPEQIGAWLARARDTEA
                     WAARARPLEIVLYPDPQVDPVGDPEGTEDLIGRYLEVGATMLNLRFRARSLQDGVDQL
                     EAMAARPAFTPSS"
     gene            complement(367..1218)
                     /locus_tag="VFP02_03005"
     CDS             complement(367..1218)
                     /locus_tag="VFP02_03005"
                     /inference="COORDINATES: protein motif:HMM:NF024107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9542022.1"
                     /translation="MARRATAVVGVSLLTLALAACGSGDDGDERLRAEPAVEEAGLPI
                     RSEERTVAGMRTVVVRPRQHGPWPLLVWVHGPEAPPESYADLLDEIAAVGNVVVAPAM
                     PGNAGADAVLALPFQPGRVRQVIDAVTTGPYAIAVADPEHIAVAGHGLGAMTALAAGF
                     NSCCVDRRVDAVVAVAGRLATFPGGIYSSGSIPVLLVHGARDGVVPYTGSGEALQQVG
                     TSAYLLTVKRGDHDSYLRADAAVHPAVRDATVAFLHATIGGSPQAGLADLIHAGGRPG
                     VRLTSRG"
     gene            complement(1223..>2242)
                     /locus_tag="VFP02_03010"
     CDS             complement(1223..>2242)
                     /locus_tag="VFP02_03010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET9542023.1"
                     /translation="VSQVGEQSNNVARNAWIAGGLPWHVPATSVDRQCGSSQQAVHFV
                     AQGVIAGAYDLGIACGVESMTRTPMGSNASGGTGPFSGQWIEAVGGRFHPTQFEVSQI
                     LAERYGVSREDMDTYAVESHRRAQENWDNGHFAKETVPVPIKDEEGAETGELLDRDQG
                     IRPESTVESLARLPSAASWDPSIAPDITAGNASQTSDGASAMLIAERSTADALGLPYR
                     AVIRHFAVAAEDPWLVLSAPVPATKKLLERSGMTVDQFDSVECNEAFAAIALMWAREF
                     QPDMTRYNSRGGAIAIGHPLGASGVRLMTTLLNDLEATGGRFGFQTMCEGGGQANATV
                     IERVT"
BASE COUNT          307 a          800 c          826 g          309 t
ORIGIN      
        1 cccctgtgga tcggtgggcg cagctaccgc tccctgcgtc gagccgtgga gctggctgac
       61 ggctgggccc cgttcggcgt gaccccggag cagatcggcg cctggctggc ccgagcccgg
      121 gacaccgaag cctgggcggc gcgggcgcgg ccgttggaga tcgtcctcta ccccgacccg
      181 caggtcgacc ccgtcggcga ccctgagggg accgaggacc tgatcggccg ctacctcgaa
      241 gtgggcgcca ccatgctcaa cctgcgcttc cgggcccgct ccctccaaga cggcgtcgac
      301 caactcgagg ccatggccgc ccgcccggcg ttcacgccct cgtcgtgagc gcgcagccgc
      361 ccggcgtcac ccccgcgacg tcaaccggac gccagggcgc ccgccggcgt ggatgaggtc
      421 ggccagtccg gcttgtggcg agccgccgat ggtggcgtgg aggaaggcca cggtcgcgtc
      481 ccggacggct ggatggaccg ccgcgtcggc ccggaggtag ctgtcgtgat caccccgttt
      541 cacggtcagc aggtaggccg aggtccccac ctgctggagg gcttcgccac tcccggtgta
      601 gggcacgaca ccgtccctgg ccccgtggac gagcaagacg ggtatggagc cgctgctgta
      661 gatgccaccc gggaaggtgg ccagccggcc ggccacagcc acgacggcat ccacccggcg
      721 gtcgacacag cagctgttga accctgcggc cagggcggtc atggccccca ggccgtggcc
      781 ggcgacggcg atgtgctcgg gatcggcgac ggcgatggcg tacgggccgg tcgtcaccgc
      841 atcgatgacc tgacgcacgc gacccggctg gaacggcagg gccagcacgg catccgcgcc
      901 agcgttgccg ggcatggccg gagccaccac gacgttgccg acggccgcga tctcgtcgag
      961 caggtccgcg taggactcgg gcggggcctc gggtccatgg acccacacca gcaggggcca
     1021 cgggccgtgc tgacgggggc ggaccaccac cgtgcgcatc ccggccacgg tccgctcctc
     1081 actgcggatg ggtaggccgg cctcctcgac ggcgggttcg gcgcgcagcc gctcatcgcc
     1141 gtcgtcgccg ctcccgcagg ccgcgagcgc gagcgtcagg aggctgaccc ccacgaccgc
     1201 cgtcgcccgc cgcgccatgg gctcaggtca cccgttcgat caccgtggcg ttggcctgcc
     1261 cgcccccctc acacatggtc tggaagccga agcgaccacc cgtggcctcg aggtcgttga
     1321 gcagcgtggt catcagccgc accccgctgg ccccgagggg gtggccgatg gcgatggctc
     1381 caccccgaga gttgtagcgc gtcatgtcag gctggaactc gcgggcccac atgagggcga
     1441 tggcggcgaa ggcctcgttg cactccaccg agtcgaactg gtcgacggtc atgcccgacc
     1501 gctcgagcag cttcttggtg gccgggaccg gggcggaaag caccagccac gggtcctcgg
     1561 cggcgacggc gaagtggcgg atgacggccc ggtacggcag gccgagggcg tcggccgtgc
     1621 tccgctcggc gatgagcatg gccgaggcgc cgtcggaggt ctgggaggcg ttgcccgccg
     1681 tgatgtcggg ggcgatcgaa ggatcccagg acgccgcgct gggcagccgg gccagcgact
     1741 cgacggtgct ctcggggcgg atgccctggt cccggtcgag cagctcgccg gtctcggccc
     1801 cctcctcgtc cttgatcggg acgggcacgg tctccttggc gaagtggccg ttgtcccagt
     1861 tctcctgggc ccgacggtgg gactcgacgg cgtaggtgtc catgtcctcg cggctgacgc
     1921 cgtaacgctc ggccaggatc tgactgacct cgaactgggt ggggtggaac cggccaccga
     1981 cggcctcgat ccactgcccc gagaacggcc cggtgccccc gctggcgttg gagcccatgg
     2041 gcgtgcgggt catggactcg acaccgcagg cgatgccgag gtcgtaggcc ccggcgatga
     2101 cgccttgggc gacgaagtgg acggcctgct gggacgagcc gcactgccgg tcgaccgagg
     2161 tggcgggcac gtgccagggc aggccaccgg cgatccaggc gttgcgggcc acgttgttgg
     2221 actgctcgcc cacttggctg ac
//
LOCUS       DASTFV010000159         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_558706, whole genome shotgun sequence.
ACCESSION   DASTFV010000159 DASTFV010000000
VERSION     DASTFV010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558706"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VFP02_03015"
     CDS             <1..966
                     /locus_tag="VFP02_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET9542024.1"
                     /translation="EIGPVLAPEAIVINKSTVPVGSTGVVERALERSDVYVVSNPEFL
                     REGSAVQDFLHPDRVVIGADDQSAAIRVASLYVGVPAPLMVTDPASAETIKYTSNAFL
                     ATKLSFINAVATICEQVGADVNDVVLGMGYDKRIGHDFLRPGPGYGGSCFPKDTSALV
                     RIAEDAGYDFALLRSAMAVNEDQFDRVASKVAELVGGDLSGCRVALWGLTFKARTDDR
                     RESPALHIARRLLDAGAVLQAHDPTVGDGPLAELPAIEVTSDPYAACEGAEALVVLTE
                     WDEFRWLDFDKVASIMASPRIMDTRNLLERAALVRRGFVYEGLGR"
     gene            970..1911
                     /locus_tag="VFP02_03020"
     CDS             970..1911
                     /locus_tag="VFP02_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HET9542025.1"
                     /translation="MARVVVTGGAGFLGSHISTALLERGDQVVAVDNLSTGRVENIEH
                     LFGNDAFQFVRRDVSEYLWVPGPVDAVLHFASPASPRDYLELPIQTLKVGSLGTHNGL
                     GLAKDKGARFLLASTSEVYGDPQIHPQPEDYWGHVNPVGPRGVYDEAKRFAEAISMAY
                     ARTHGMAVRIVRIFNTYGPRLRARDGRVVSNFIDQALRGEPLTVYGDGAQTRSFCYVE
                     DEVRGLLALLDSDLDGPCNIGNPDEFTVRELADLVIEVTDSTSEVVHEDLPVDDPAKR
                     RPDITRARTELGWEPKVQLREGIERTVEWFKDELDRG"
     gene            complement(1917..>2502)
                     /locus_tag="VFP02_03025"
     CDS             complement(1917..>2502)
                     /locus_tag="VFP02_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9542026.1"
                     /translation="QLWTGYPEETNAPYGIAKLAQLAQAQANREQYGQDVIYLMPTNL
                     YGPGDKFNASVSHVIPALIKKCVDAHDAGEDHIVVWGTGNASREFLYVDDAADAILQA
                     AERYDGGDPVNLGADEETWIRDLVPLIAETVGFEGEIRWDSSYPDGQPRRRVDPTRAA
                     TEFGFKASTPLRDGLRATVDWYLSNREEAEARIL"
BASE COUNT          362 a          886 c          847 g          407 t
ORIGIN      
        1 gagatcggcc ccgtgttggc ccccgaggcg atcgtcatca acaagtcgac ggtgcctgtc
       61 ggttcgaccg gggtggtcga gcgggccctg gagcgctccg acgtgtacgt ggtgtccaat
      121 ccggagttcc tccgcgaagg ctcggcggtg caggacttcc tgcaccccga ccgggtggtc
      181 atcggggccg acgaccagag cgccgccatc cgggtcgcgt ccctctacgt gggagtcccg
      241 gcgccgctca tggtcaccga cccggcgtcg gctgagacca tcaagtacac cagcaacgcc
      301 ttcctggcca ccaagctctc gttcatcaac gccgtggcca ccatctgcga gcaggtgggg
      361 gctgacgtca acgacgtcgt gctcggcatg ggttacgaca agcggatcgg gcacgacttc
      421 ctccggcccg gcccgggcta cggcggctcg tgcttcccga aggacacctc ggcgctggtt
      481 cgcatcgccg aggacgcggg ctacgacttc gccttgctcc gcagcgcgat ggccgtcaac
      541 gaggaccagt tcgaccgcgt cgcgtccaag gtcgcggagc tggtgggcgg ggacctgtcg
      601 gggtgtcgcg tcgccctgtg gggcctcacc ttcaaggccc gcaccgacga ccggcgggag
      661 tcacccgccc ttcacatcgc ccgtcgcctg ctggacgccg gcgccgtgct ccaggcccac
      721 gaccccaccg tcggcgacgg tccgctggcc gaactgcccg ccatcgaggt gacgagtgac
      781 ccgtatgccg cctgtgaggg ggcggaggcc ctcgtcgtgc tgaccgagtg ggacgagttc
      841 cgctggctgg acttcgacaa ggtggcgtcg atcatggcca gtccccgcat catggacacc
      901 cgcaacctgc tcgagcgcgc cgccctcgtc cgccggggct tcgtctacga gggcctcggg
      961 cgctgatcca tggcccgggt cgtggtgacg gggggagccg ggttcctcgg ctcccacatc
     1021 agcaccgccc tcctcgagcg gggtgaccag gtcgtcgccg tcgacaacct gagcaccggt
     1081 cgggtcgaga acatcgagca tctcttcggc aacgacgcgt ttcagttcgt ccgtcgcgac
     1141 gtctccgagt acctgtgggt ccccggcccg gtcgatgctg ttctgcactt cgccagcccg
     1201 gcctcgcccc gcgactacct ggagctgccc atccagaccc tcaaggtggg cagcctcggc
     1261 acccacaacg ggctgggcct cgccaaggac aagggcgccc ggttcctgct cgcctcgacc
     1321 agcgaggtct acggcgaccc ccagatccat ccccaacccg aggactactg gggccatgtg
     1381 aacccggtcg gtccccgggg cgtgtacgac gaggccaagc gcttcgccga ggccatctcg
     1441 atggcctacg cccggaccca cggcatggcg gtccgcatcg tccgcatctt caacacctac
     1501 ggcccccggc tccgggcccg cgacggacgg gtggtgtcca acttcatcga ccaggccctc
     1561 cggggcgagc cgctgaccgt ctacggcgac ggcgcccaga ctcggagctt ctgttacgtc
     1621 gaggacgagg tccgggggct gctggccctc ctcgacagtg acctcgatgg tccgtgcaac
     1681 atcggcaacc ccgacgagtt caccgtccgg gagctggccg acctcgtcat cgaggtgacc
     1741 gacagcacgt cggaggtcgt ccacgaggac ctgcccgtcg acgaccccgc gaagcgccga
     1801 cccgacatca cccgagcccg caccgagctc ggatgggagc ccaaggtcca gctccgcgag
     1861 ggcatcgagc gcaccgtgga gtggttcaag gacgagctcg accggggctg accgccctac
     1921 aggatcctcg cttcggcctc ttcgcggttg ctcagatacc agtccacggt ggctcggagg
     1981 ccgtcccgga ggggggtgct cgccttgaag ccgaactcgg tggcggcccg cgtgggatcc
     2041 acgcggcggc ggggttggcc gtcggggtag ctgctgtccc agcggatctc gccttcgaag
     2101 ccgacggtct cggcgatgag ggggaccagg tcccgtatcc acgtctcctc gtcggcgccg
     2161 aggttcaccg ggtcaccgcc gtcataccgc tcggctgcct ggaggatggc gtcggcggcg
     2221 tcgtcgacgt acaggaactc ccggctggcg ttgccggtcc cccagacgac gatgtggtcc
     2281 tcaccggcgt cgtgggcgtc gacgcacttc ttgatgaggg cggggatcac gtgggacacc
     2341 gacgcgttga acttgtcgcc cgggccgtag aggttggtcg gcatcaggta gatgacgtcc
     2401 tggccgtact gctcccggtt ggcctgggcc tgggccagtt gagccagctt ggcgatgccg
     2461 tagggggcgt tggtctcctc gggatagccc gtccacagct ga
//
LOCUS       DASTFV010000160         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_559814, whole genome shotgun sequence.
ACCESSION   DASTFV010000160 DASTFV010000000
VERSION     DASTFV010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559814"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VFP02_03030"
     CDS             <1..344
                     /locus_tag="VFP02_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9542027.1"
                     /translation="QDPLSLDSPVGEEDDSNLADFIEDLQAEAPADVATRHMLNDAVL
                     EALDELTPREKEVVRLRFGLEDGQARTLEEVGKQFGVTRERIRQIEAKTLAKLRHPHR
                     SQKLRDYLEGG"
     gene            complement(355..558)
                     /locus_tag="VFP02_03035"
     CDS             complement(355..558)
                     /locus_tag="VFP02_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542028.1"
                     /translation="MGFRTGVIVGLAAGYYLGAKAGRERYEQINAWIDQARNSDAVNA
                     AAGRARELIDDADDLTAANFSNN"
     gene            680..755
                     /locus_tag="VFP02_03040"
     tRNA            680..755
                     /locus_tag="VFP02_03040"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:713..715,aa:Asn,seq:gtt)
     gene            788..1252
                     /locus_tag="VFP02_03045"
     CDS             788..1252
                     /locus_tag="VFP02_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HET9542029.1"
                     /translation="MTMVDLPKAVMRSESDCPMVDAGEGIKLQLLQVDVELGLWVIRN
                     LFEPGARVQTHKHTGQVFAFTQSGCWKYDEYPERMTAGTYLFEPAGSIHTLIVPEDNT
                     EVTDVWFAIYGANLNLDAEGNVESVLDAAFIRDAYLALCEAEGHPRPNVIGA"
     gene            1262..2269
                     /locus_tag="VFP02_03050"
     CDS             1262..2269
                     /locus_tag="VFP02_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9542030.1"
                     /translation="MRAVVLTTLGGPLETAEVDDPAPGPGEVVVAVDSCGICGSDLHI
                     VDAFAIPGQVLGHEVAGRVAAVGQGVEGWREGDPVMALSLATCGTCDACRSGRPRKCT
                     AALMMGVETPGAYAEYVRTPAHDLVAMPAGFDLRLGALVEPLAVARHAVGRGGLMAGE
                     SALVIGGGPVGLAVLVWLKHLGAGTVAVTDPRASRRAGARQLGADVVLDPTAGDLAGQ
                     LAHAGVDAPTLVLECVGLPGLVDQAAAVAAVDGRVVVVGVCMADDRYFPYTAIAKELD
                     WRFAFYYCRADVDATVAAIADGLDVAPLITGEVDLAATPARFDALKTGADDTKVLIRP
                     G"
BASE COUNT          337 a          870 c          813 g          358 t
ORIGIN      
        1 cccaggaccc gctgtcgctg gactcaccgg tgggggagga ggacgactcc aacctggccg
       61 acttcatcga ggacctacag gcggaggcgc cggccgacgt cgccacccgt cacatgctca
      121 acgacgccgt gctggaggcg ctggacgagc tgaccccccg ggagaaggag gtcgtgcgcc
      181 tgcgtttcgg cctcgaggac ggccaggccc gcacgctgga agaggtgggc aagcagttcg
      241 gcgtgacccg cgagcggatc cgccagatcg aggccaagac cctggccaag ctgcgccacc
      301 ctcaccgcag ccagaagctg cgcgactacc tcgagggtgg ctgagggctg agccctagtt
      361 gttcgagaag ttggcggccg tgaggtcgtc agcgtcgtcg atgagctccc gggcccggcc
      421 ggcggcggcg ttgaccgcgt cggaattgcg ggcctggtcg atccacgcgt tgatctgctc
      481 gtagcgctcc cggcccgcct tggcgccgag gtagtacccc gccgccaggc cgacgatcac
      541 gcctgtcctg aatcccatgc cccaatgctt actccctcag gggggtttgt gacaccccaa
      601 tcggcacttc ttcgcgatcg acgtgtctgc ctttgctcat caggggactg atcgacgcgc
      661 tgttatcctc cgaggccatt ccggggtagc tcagctggca gagcaatcgg ctgttaaccg
      721 atttgtcgtg ggttcgagcc ccacccccgg agccagccgc attgcccccg aactaacctg
      781 cgccgccatg acgatggtgg atctgcccaa ggccgtgatg cgcagcgagt cggactgccc
      841 gatggtggat gccggcgagg gcatcaagct ccagctgctc caggtcgacg tcgagctcgg
      901 cctctgggtc atccggaacc tgttcgagcc cggggcccgc gtgcagacac acaagcacac
      961 cggacaggtc ttcgccttca cccagtcggg ctgctggaag tacgacgagt accccgagcg
     1021 gatgaccgcc ggcacctacc tcttcgagcc cgcgggctcg atccacacgc tgatcgtgcc
     1081 cgaggacaac accgaggtga ccgacgtctg gttcgccatc tacggggcca acctcaacct
     1141 cgacgcggag ggcaacgtgg agtcggtcct cgacgccgcc ttcatccgag acgcctacct
     1201 cgccctctgc gaggccgagg gccacccccg cccgaacgtc atcggcgcct gagggcacgc
     1261 cgtgcgggcc gtggtgctga ccacgctcgg cggcccactg gagaccgccg aggtcgacga
     1321 cccggccccc ggccccggtg aggtcgtcgt cgccgtcgac tcctgcggga tctgcggctc
     1381 cgacctccac atcgtcgacg ccttcgccat acccggccag gtactgggcc atgaggtggc
     1441 cgggcgggtc gccgccgtcg gtcagggcgt cgagggctgg cgggaaggcg atcccgtgat
     1501 ggccctgtcc ctggccacct gcggcacgtg cgacgcctgc cggagcggcc gaccccgcaa
     1561 gtgcaccgcg gccctgatga tgggggtcga gaccccgggc gcctacgccg agtacgtcag
     1621 gacgccggcc cacgatctgg tcgccatgcc cgcgggcttc gatctccgcc tcggcgccct
     1681 cgtcgagccc ctcgccgtgg cccgtcacgc cgtgggccgc gggggcctga tggcggggga
     1741 gtcggcgctg gtcatcggcg gcgggcccgt cggcctcgcc gtcctcgtct ggttgaagca
     1801 cctcggggcc ggcaccgtcg ccgtgaccga cccgcgggcg agccgccggg ccggcgcccg
     1861 ccagctcggc gccgacgtgg tcctcgaccc gacggcggga gacctcgccg gacaactcgc
     1921 ccacgccggc gtcgacgcgc cgacgctggt cctggagtgc gtgggcctgc cgggtctggt
     1981 cgaccaggcg gcggcggtcg ccgcggtcga cggccgggtg gtggtggtcg gcgtctgcat
     2041 ggccgacgac cggtatttcc cttacaccgc gatcgccaag gagctggact ggcggttcgc
     2101 cttctactac tgccgggccg acgtcgacgc cacggtcgcg gccatcgccg acggcctcga
     2161 tgtcgccccg ctgatcaccg gcgaggtcga cctggccgcc acccccgccc gcttcgacgc
     2221 gctcaagacc ggcgccgacg acaccaaggt cctcatccgc cccggctgac cttccgacct
     2281 gaccggcgtg gacttgtcgc tcgttggagc cgatagcgcg ggcccgccgt caccggcgcg
     2341 gtagaaagcc cgtgggcccg tagctcaatg gtcagagc
//
LOCUS       DASTFV010000161         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_559868, whole genome shotgun sequence.
ACCESSION   DASTFV010000161 DASTFV010000000
VERSION     DASTFV010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559868"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VFP02_03055"
     CDS             <1..619
                     /locus_tag="VFP02_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9542031.1"
                     /translation="ETVLIHAAAGGSGSAAIQLAVDAGAHVIATVGSEEKVQLCRDLG
                     AETVVNYRTDAFAPVVMEQTGGKGADVVFDNVGDAVMADSLRCTAYNGRYLMMGFASD
                     KTVADEKSIVPRTIALANIKLCGVLLSYADADFGAAIKQAMGWNFPSNELGAEIMQKV
                     IDLVREERVRAVVGRVADFDEVPAAFEAMANRETVGRTIVKLWPD"
     gene            733..2034
                     /locus_tag="VFP02_03060"
     CDS             733..2034
                     /locus_tag="VFP02_03060"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9542032.1"
                     /translation="MALVLLAGACSSQAADDSSSGGGGSHDGSGDAAADGGTPIDGLT
                     DDTINVSFIGADFAALAEAGLAPDLGDMTVTIPALVDDINENGGIGGRQINLTVDLVD
                     GTAGPDVGQAACIKATDEDDAAVVFVSPAISRDIARCTAVQQQTVTLGMPGWDDPLYE
                     EAEGRLFSVGSQTSMGTYRNTAVWADVLDQAGELEGKTIGVVTSDDFEALAAGTEQAL
                     VPHLEELGYDVVEFAVLPCPEGDTDCEQHEPTMQTFKDAGVDLVFMNAGALAGPALFA
                     AATSLDYHPQWVTCCNSITDTVVQFAAGSFDQLDGTFGVSTTFAQRSEEAETCNQIVR
                     DRTDEDYATESDAYGFTGVNCIALLVFRDAMDTIDGPVTESALIEAIEGLETVPSLEG
                     IPGTIGPDKHDAGDAVFLAQFTTEAGEYLPVDDEEPIEAPA"
     gene            2048..>2298
                     /locus_tag="VFP02_03065"
     CDS             2048..>2298
                     /locus_tag="VFP02_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542033.1"
                     /translation="MTGGEAEDRGLAAAVLEEESRRDAARAPAPPVVLPDDLLPGVGG
                     EEMPLREALRIGGWTTVITIGLAGALIGFDNVALAVLSP"
BASE COUNT          364 a          836 c          797 g          301 t
ORIGIN      
        1 cgagaccgtc ctcatccatg ccgcggccgg gggctcgggc tcggccgcga tccagctggc
       61 ggtcgacgcc ggggcccacg tgatcgccac cgtcgggtcc gaggagaagg tgcagctctg
      121 ccgcgacctc ggcgccgaga ccgtcgtcaa ctaccgcacc gatgcgttcg cccccgtcgt
      181 gatggagcag accggcggca agggggccga cgtcgtcttc gacaacgtgg gcgacgcggt
      241 catggccgac tcgctgcgct gcaccgccta caacggccgc tacctcatga tggggttcgc
      301 ctcggacaag acggtcgccg acgagaagtc gatcgtcccc cgcaccatcg ccctggccaa
      361 catcaagctc tgcggcgtgc tcctgtcgta cgccgacgcc gacttcggag ccgccatcaa
      421 gcaggcgatg ggctggaact tcccgagcaa cgagctcggg gccgagatca tgcagaaggt
      481 catcgacctc gtccgggagg agcgggtgcg ggccgtggtc gggcgggtcg ccgacttcga
      541 cgaggtcccc gccgccttcg aggcgatggc caaccgcgag accgtcggcc gcacgatcgt
      601 gaagctctgg ccggactgac gggccgaccc cgccgcccgc gacggaccgt gggcgatttc
      661 cgaactattg tcggaaactc acgacggcct ccggggggat gcatgacggg acaccgatgg
      721 accgcagggc tggtggccct cgtactgctg gccggggcct gcagcagcca ggccgccgac
      781 gacagcagta gcggtggcgg tggcagccac gacgggagcg gtgacgccgc cgccgacgga
      841 ggaacgccca tcgacggcct caccgacgac accatcaacg tgtcgttcat cggcgccgac
      901 ttcgccgcgc tggccgaggc ggggctggct cccgatctcg gcgacatgac ggtcaccatc
      961 cccgccctcg tcgacgacat caacgagaac ggcggcatcg gtggccgcca gatcaacctg
     1021 accgtcgacc tcgtcgacgg caccgccggc ccggacgtag gtcaggccgc gtgcatcaaa
     1081 gccaccgacg aggacgacgc ggccgtcgtg ttcgtctccc ccgccatcag ccgggacatc
     1141 gcccgctgca ccgcggtcca gcagcagacg gtcacgctgg gcatgccggg ttgggacgac
     1201 ccgctctacg aggaggccga gggtcggctc ttcagcgtgg gctcccagac ctccatgggg
     1261 acctaccgga acaccgcggt ctgggccgac gtcctcgacc aggccggtga gctggagggc
     1321 aagaccatcg gcgtggtcac cagcgacgac ttcgaagcgc tggccgcggg cacggagcag
     1381 gcgctcgtgc cccatctcga ggagctcggc tacgacgtgg tcgagttcgc cgtgctcccc
     1441 tgcccggagg gcgacacgga ctgcgagcag cacgagccga ccatgcagac cttcaaggac
     1501 gccggcgtcg acctcgtctt catgaacgcg ggtgccctcg ccggcccggc gctgttcgcc
     1561 gccgcgacca gcctcgacta ccacccccag tgggtcacct gctgcaacag catcaccgac
     1621 accgtcgtcc agttcgcggc ggggtccttc gaccagctcg acggcacctt cggcgtcagc
     1681 acgacgttcg cccagcgctc cgaggaggcc gagacctgca accagatcgt gcgggaccgc
     1741 accgacgagg actacgccac cgagtcggac gcctacggct tcaccggcgt caactgcatc
     1801 gccctgctcg tcttccgtga cgcgatggac accatcgacg gcccggtgac cgagtcggcg
     1861 ctcatcgagg ccatcgaggg cctggagacc gtgccctccc tggagggcat cccgggcacc
     1921 atcggccccg acaagcacga cgccggggac gcggtgttcc tggcccagtt cacgacggag
     1981 gcgggcgagt acctgcccgt cgacgacgag gaaccgatcg aggcgccggc ctgaccgggc
     2041 cgcgagcgtg accggcgggg aggcggagga ccgggggctg gcggccgccg tcctggagga
     2101 ggagtcccgc cgcgacgcgg cccgcgcccc ggccccgccc gtcgtcctgc ccgacgacct
     2161 cctgcccggg gtcggcggtg aggagatgcc gctgcgcgaa gcgctgcgga tcgggggctg
     2221 gacgaccgtc atcaccatcg ggctggccgg ggcgctcatc ggcttcgaca acgtcgccct
     2281 ggcggtgctc agcccgga
//
LOCUS       DASTFV010000162         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_560155, whole genome shotgun sequence.
ACCESSION   DASTFV010000162 DASTFV010000000
VERSION     DASTFV010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560155"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFP02_03070"
     CDS             complement(<1..543)
                     /locus_tag="VFP02_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9542034.1"
                     /translation="MFLKSLSMKGFKSFADATRLDLEPGITVVVGPNGSGKSNVVDAI
                     GWVLGAQAPSIVRSKAMDDVIFAGTSQRPALGRAEVALTIDNSAGLLPIEFTEVTISR
                     TLFRSGDSEYAINNVPCRLLDIQDLLSDSGVGRQQHVIVSQGQIDAVLNAKAEDRRAI
                     IEEAAGILKYRKRKERSERRL"
     gene            complement(675..1511)
                     /gene="mutM"
                     /locus_tag="VFP02_03075"
     CDS             complement(675..1511)
                     /gene="mutM"
                     /locus_tag="VFP02_03075"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HET9542035.1"
                     /translation="MPELPEVETIRRELERDIVGLRIKDVEVNDLRSIRRHGKKAPFV
                     KLLDGAKVTHVHRRGKYLVLSLDTGSVLVIHLGMSGQLRRNAAKDAAEPHTRVVVTFT
                     QKGQLRFIDPRKFGEMFVVDADAVDDEVPELAHLGFDPVEQAVSWTRFGQALVNRSVK
                     LKPLLMDQKFIAGIGNIYADEILYGAGLRYDRLSDSLSTQEIRRLYRSLVETLHDAIK
                     YRGSTLADGQYVDLQGKPGEYQEHHQVYDREGQACRRCRSVIVRVKANSRSTFFCEQC
                     QV"
     gene            complement(1504..2253)
                     /gene="rnc"
                     /locus_tag="VFP02_03080"
     CDS             complement(1504..2253)
                     /gene="rnc"
                     /locus_tag="VFP02_03080"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET9542036.1"
                     /translation="MTERAMSDLAARLGHDFADPGLLVEAVTHRSWCAEHVGTPSNER
                     LEFLGDAVLGLVIADYLFQVEGAMPEGELAMARAAVVSTEALAQVASVLELGQFLRLG
                     KGEDATGGREKRSILADSMEAIIGAVYVDAGLAAARTLLLPLVAERLAGAVAGPGEAD
                     HKTRLQEASARQYGQVPRYEVSGTGPDHDRRFTAVVRIGGTERGQGTGKSKKEAEQAA
                     ARVAWQSLRDEPEPTNEPESTTETESRTADA"
     gene            complement(2288..2578)
                     /locus_tag="VFP02_03085"
     CDS             complement(2288..2578)
                     /locus_tag="VFP02_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HET9542037.1"
                     /translation="MPAETHVEQGPMGRPEVLELIRDRLADILEIEPSQIQEGDSFAD
                     DLDADSLALIELVEALEEELSERSVGFRIEDEDLEDLKSVRDAVDYVVARLG"
     gene            complement(2693..>2994)
                     /locus_tag="VFP02_03090"
     CDS             complement(2693..>2994)
                     /locus_tag="VFP02_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542038.1"
                     /translation="LKTLEGGLKAIVGAIYEVFGSSDEARAAADVLLPALIPLADQLD
                     PDSTGGAMLLGVDGVCIISHGSSSARAVVNAVRVGAEMAEADLVGDLTAAIRPPD"
BASE COUNT          416 a         1017 c         1072 g          489 t
ORIGIN      
        1 caggcggcgc tcggagcgct ccttgcgctt gcggtacttg aggatgccgg cggcctcctc
       61 gatgatcgcc cgccggtcct cggccttggc gttgaggacg gcgtcgatct ggccctggct
      121 gacgatgacg tgctgttggc ggccgactcc cgagtccgac agcaggtcct ggatgtcgag
      181 caggcggcag gggacgttgt tgatggcgta ctcgctgtca cccgaacgga acagcgtgcg
      241 gctgatggtc acctcggtga actcgatggg cagcaggccc gccgagttgt cgatggtgag
      301 ggccacctcg gcccggccca gggccgggcg ctgggaggtc ccggcgaaga tgacgtcgtc
      361 catcgccttg gagcgcacga tgctcggggc ctgggcgccg agcacccagc cgatggcgtc
      421 caccacgttg gacttgccgc tcccgttggg cccgacgacc acggtgatgc ccggctcgag
      481 gtcgaggcgg gtggcgtcgg cgaaggactt gaagcccttc atggacagtg acttcaggaa
      541 cacgtgcgca ccccgggaac agtgatgctc cggacgttag ccagcagcct cgggctggtc
      601 gcgcacccca gagacctcag gtggcgccgg ggtttcccgt gtccggacca cggcggcgcc
      661 gacgggcttg ccggtcacac ctggcactgc tcgcagaaga acgtgctgcg gctgttggcc
      721 ttgacccgga cgatgaccga gcggcagcga cggcaggcct ggccctcgcg gtcgtagacc
      781 tggtggtgct cctggtactc gccgggcttg ccctggaggt ccacgtactg gccgtcggcg
      841 agggtcgagc cgcggtactt gatggcgtcg tggagggtct cgaccaggga ccggtacagc
      901 cgccggatct cctgggtgga cagcgagtcc gagagccggt cgtagcggag gccggcgccg
      961 tagaggatct cgtcggcgta gatgttgccg atgccggcga tgaacttctg gtccatcagc
     1021 aggggcttga gcttcacgct gcggttgacc agcgcctgac cgaaccgggt ccaggacacg
     1081 gcctgctcga ccgggtcgaa gccgaggtgg gccagctcgg ggacctcgtc gtcgacggcg
     1141 tcggcgtcga ccacgaacat ctccccgaac ttgcgggggt cgatgaagcg cagctggccc
     1201 ttctgggtga aggtgacgac gacccgggtg tgcggctcgg ccgcgtcctt ggccgcgttg
     1261 cgccggagct ggccgctcat gcccaggtgg atgaccagca cgctcccggt gtccagggac
     1321 aggaccaggt acttcccgcg gcggtggaca tgggtgacct tcgcgccgtc gagcagcttg
     1381 acgaacggcg ccttcttgcc gtggcggcgg atcgaacgca ggtcgttgac ctcgacgtcc
     1441 ttgatccgga gccccacgat gtcgcgctcc agctcccgcc ggatggtctc cacttcggga
     1501 agctcaggca tcggcggtac ggctctcggt ttcagtcgtg gactcgggtt cgttcgtggg
     1561 ttcgggctcg tcccggaggg actgccaggc gacccgggcg gcggcctgtt cggcctcctt
     1621 cttggacttg ccggtgccct ggccccgctc ggtcccgccg atgcggacca cggcggtgaa
     1681 gcgacggtcg tggtccggtc cggtgcccga cacctcatag cggggcacct ggccgtactg
     1741 gcgggccgac gcctcctgga gccgggtctt gtggtcggcc tcgcccggcc cggcgaccgc
     1801 cccggcgagg cgctcggcca ccaggggcag cagcagcgtg cgggcggcgg cgaggccggc
     1861 gtcgacgtag acggcgccga tgatcgcttc catggagtcg gccaggatcg agcgcttctc
     1921 ccgtccgccc gtggcgtcct cccccttgcc caggcggagg aactggccca gctcgaggac
     1981 cgaggccacc tgggccaggg cctcggtgct caccacggcg gcccgggcca tggccagctc
     2041 cccctcgggc atggccccct cgacctggaa gaggtagtcg gcgatgacca gtccgaggac
     2101 ggcgtcgccc aggaactcga ggcgctcgtt ggacggggtg ccgacgtgct cggcacacca
     2161 agagcgatgg gtgacggcct cgaccaggag gccggggtcg gcgaagtcgt ggcccaggcg
     2221 cgccgcgagg tcgctcatgg cgcgctcggt catgtccaca gatccgtcct cacgtcatcg
     2281 ccggacctca gccgaggcgg gcgaccacgt agtccacagc gtcgcggacc gacttgaggt
     2341 cctcgagatc ttcgtcctcg atccgaaacc ccacggagcg ctccgagagc tcctcttcga
     2401 gggcttcgac gagctcgatc agggccaggg agtcggcatc caggtcgtcg gcgaaggaat
     2461 cgccctcctg gatctggctc ggctcgatct cgaggatgtc ggccagacgg tcacggatga
     2521 gctccagcac ctcgggtcgc cccatcgggc cctgttcgac atgggtctcg gccggcacgt
     2581 gcgcactctc cccgttcgct cgttcgtggt gggtccgacg attctatcgc cgccgacacc
     2641 cactcgcagg gtgcgcccca tagcctttcc gcccttttgt cgacgtctca ggtcaatcgg
     2701 gtgggcggat ggccgcggtg aggtcgccga ccaggtcggc ctcggccatc tcggccccga
     2761 cccgcaccgc gttgacgacg gctcgggccg acgacgaccc gtgactgatg atgcagaccc
     2821 cgtcgacccc cagcagcatc gcccctcccg tggaatccgg gtccagctgg tcggccaggg
     2881 ggatcagcgc ggggaggagc acgtccgccg cggccctggc ctcgtccgac gagccgaaga
     2941 cctcgtagat ggccccgacg atggccttca gcccaccctc gagggtcttg aggg
//
LOCUS       DASTFV010000163         4461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_574710, whole genome shotgun sequence.
ACCESSION   DASTFV010000163 DASTFV010000000
VERSION     DASTFV010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574710"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2387
                     /locus_tag="VFP02_03095"
     CDS             <1..2387
                     /locus_tag="VFP02_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9542039.1"
                     /translation="WFCGFLLIALAARPAQPTELADDLDGVHTRWLTELVIHFPVVSV
                     GALAVVERVTGGEVSNGTIALILVVFVLNALRHAAASRQMAGLTGALAEQLSALQTER
                     QRSDLVIDEARDPYVELDPDGRVLVWNGPAEAVFGWSRDHMVGRLIADIVPEDNREAS
                     RLRMEEFIASSDASVWDEGVDVRYLRPDGTEVPIEIAAWSTGEGAALRVHAFVWDISE
                     RLAEHELLEASEKKWQSLVSHASDMTIVLDREGRVDQEVPTVRQLFGYADNENHGRHF
                     ADLIHPQDLQRAIDRFTRVLAEPGNQERERVRIRRFDGSWVHVDAIATNLLDEPTVAG
                     IVCNLRDISEQVAAENELKRQARRDPLTGLPNRNLMTNALARHCAEEHDGHAALLLMD
                     LDGFKDINDGLGHDVGDQLLVEIGHRLDRVTRADDVVARLGGDEFAVLVTRLPALGQA
                     AAIAENLGNLIREPVRLGDVTLEVGVSIGIADCSPGDDPLAVLQRADVAMYRAKRERS
                     GHAVYGSGDDEERRNSVKVAADLRQAIDRGDLVVHYQPKIELSTGLVDSVEALVRWQH
                     PTRGLLPPDEFIALAERTGLIRDLTTWVLGTALRQCATWRAEGLDLPVAVNLSPRVLG
                     QSDLVAGVREALEANGVPAGRLTLEITESAFPENGRLLSSTLDALGRLGVRLSVDDFG
                     TGWSSLSKLKEMPVQELKIDRTFVFALAEDATDHSIVRSVLELARSLGLTVVAEGVET
                     EEVLDLLRELDCPLVQGYLFSRPVPGHELTGWILERDHQRRTTGSTDAEDSFPH"
     gene            complement(2373..3083)
                     /locus_tag="VFP02_03100"
     CDS             complement(2373..3083)
                     /locus_tag="VFP02_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HET9542040.1"
                     /translation="MRFGVLAPMPSELRPVVKAFGLRRGRSGSLEGHVGRAGEADVVA
                     TTTGIGTELATTAAQRMLDRGEVDHVVVVGIAGGIGPTTAVPDLIIPEAVVDWPELRE
                     HRPAPLGRLVGAGTIVSSDEYGYPPEVIADFIARGVVAVDMETVAVARVCADAEIPWS
                     AVRAISDRADDDTVDQDVITLVKPDGSPDLGRSLRYLIRHPGRISRLAALGRDASAAA
                     RVAATAAAAACAAHSVGE"
     gene            complement(3093..4298)
                     /locus_tag="VFP02_03105"
     CDS             complement(3093..4298)
                     /locus_tag="VFP02_03105"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET9542041.1"
                     /translation="MGDDGQGAAGGPLAGIKVMDLAIAATGPYAATMLADQGAEVIKV
                     ERLGIGDIARYVGVQIDGISALFQLCNRGKRSIAVDLSVEAGRDVVRALAADCDVVIQ
                     NWRPGVADRLGVGYEDLRRDDLVYVSISGFGDVGPKADRGAYDTVIQAQAGMGHAQAD
                     PDTGEPAFVRQVIADKVTALTAAQAVTAALLARERGRGGQHLRLSMLEAVVAFLWVDA
                     AGNEVLRDGDGSQPGSFSAGARPIAFEDGWGMVTPTSDADFFGMCRAFGVNGHDDPRL
                     ASPTLRQQNADLTREIVGRCWAAAAGLTVADASRRLEAERVPYGILVSPADLAEDPQA
                     VALELLVDSHHPVAGAIRQPRHPTRFEGTPAGVGGPSPALGQHTDELLAEAGLADRIV
                     ELRAAGVVG"
BASE COUNT          645 a         1667 c         1546 g          603 t
ORIGIN      
        1 cgtggttctg cgggttcctg ctcatcgccc tggcggctcg accggcgcag ccgaccgagc
       61 tcgccgacga cctcgacggc gtccacaccc gctggctcac cgagctggtc atccacttcc
      121 ccgtcgtctc cgtcggcgcg ctggccgtcg tcgaacgggt caccggtggc gaggtgtcca
      181 acggcaccat cgccctcatc ctcgtggtgt tcgtgctcaa tgccctccgc cacgccgcgg
      241 ccagccgtca gatggccggc ctgaccggag ccctcgcgga gcagctctcg gccctgcaga
      301 ccgagcgcca acgctccgac ctggtcatcg acgaggcccg cgacccttac gtcgagctgg
      361 atccggacgg gcgggtcctg gtctggaacg gccccgccga ggccgtcttc ggttggagcc
      421 gggaccacat ggtcggccgg ttgatcgccg acatcgtgcc cgaggacaac cgggaggcca
      481 gccgactgcg catggaggag ttcatcgcgt cgtcggacgc gtcggtctgg gacgaagggg
      541 tcgacgtccg ctatctccgc ccggacggca ccgaggtccc gatcgagatc gcggcgtggt
      601 ccaccggcga gggggccgcc ctccgggtcc acgccttcgt ctgggacatc agcgagcgcc
      661 tggccgagca cgagttgctg gaggccagcg agaagaaatg gcagtccctg gtcagccacg
      721 cctcggacat gaccatcgtc ctcgaccgcg agggccgggt ggaccaggag gtccccaccg
      781 tccggcaact gttcggctac gcggacaacg agaaccacgg gcgccacttc gccgacctca
      841 tccaccccca ggacctccag cgggccatcg accgcttcac ccgggtgttg gccgaaccgg
      901 ggaaccagga gcgggagcgg gtccgcatcc gccgcttcga cggcagctgg gtccacgtcg
      961 acgccatcgc caccaacctg ctcgacgagc cgaccgtggc cggcatcgtc tgcaacctgc
     1021 gggacatctc cgagcaggtg gcggcggaga acgagctgaa gcgccaggcc cgacgggacc
     1081 cgctcacggg cctgccgaac cgcaacctca tgaccaacgc gctggcccgg cactgcgccg
     1141 aggagcacga cggccacgcc gccctcctcc tcatggacct cgacggcttc aaggacatca
     1201 acgacgggct cggccacgac gtgggcgacc agctgctggt cgagatcggg caccgcctcg
     1261 accgggtgac ccgggccgac gacgtggtcg cccgcctggg cggcgacgag ttcgccgtcc
     1321 tcgtgacccg gctgccggcc ctcggccaag ccgccgccat cgccgagaac ctgggcaatc
     1381 tgatccggga gcccgtgcgc ctcggggacg tcaccctcga ggtcggggtg agcatcggca
     1441 tcgccgactg ctcccccggt gacgatcccc tggctgtgct ccagcgggcc gacgtggcca
     1501 tgtaccgggc caagcgcgag cgcagcggcc acgccgtcta cggcagtggg gacgacgagg
     1561 agcggcgcaa cagcgtgaag gtggccgccg acctccgcca ggccatcgac cggggcgacc
     1621 tcgtcgtgca ctaccagccc aagatcgagc tgagcaccgg gctggtggac tccgtcgaag
     1681 ccctggttcg gtggcagcac cccacgcggg gcctgctccc ccccgacgag ttcatcgccc
     1741 tggccgagcg caccggactg atccgggacc tgaccacgtg ggtcctggga acagccctgc
     1801 gtcagtgtgc gacctggcgg gccgagggcc tcgacctgcc cgtggccgtc aacctgtccc
     1861 ctcgggtgct gggccagagc gatctcgtgg ccggcgtccg cgaggctttg gaggccaacg
     1921 gggtacccgc cggccgcctg accctcgaga tcaccgagtc ggcgttcccc gagaacggcc
     1981 ggttgctctc ctcgaccctc gatgctctcg gccggttggg tgtgcgcctg tcggtcgacg
     2041 acttcggtac gggatggtca tcgctgtcca agctgaagga gatgccggtc caggagctca
     2101 agatcgatcg gaccttcgtc ttcgccctcg ccgaggacgc cacggaccac tcgatcgtgc
     2161 gatccgtgct cgagctggcc cggtccctgg gcctcaccgt ggtcgccgaa ggcgtggaga
     2221 ccgaagaggt cttggacctg ctccgcgagc tcgactgccc gctcgtgcag ggctatctct
     2281 tctcccggcc ggtgcccggc cacgagctca ccgggtggat cctggaacgg gaccaccagc
     2341 gccgcaccac cggctccacc gacgccgagg actcattccc ccactgagtg cgcggcacag
     2401 gcggcggccg cggccgtcgc cgccacgcgc gccgcggccg acgcgtcccg ccccagggcc
     2461 gccaggcggg agatccgtcc gggatggcgg atgaggtacc gcaggctccg gccgaggtcg
     2521 ggtgagccgt cgggcttgac cagggtgatc acgtcctggt cgacggtgtc gtcatcggcc
     2581 cgatcactga tcgcccgcac ggccgaccag gggatctccg cgtcggcgca gacccgggcc
     2641 acggccaccg tctccatgtc cacggcgacc acaccgcggg cgatgaaatc ggcgatcacc
     2701 tccggcgggt agccgtactc gtcggaggag acgatggtgc cggcgccgac caggcggccg
     2761 aggggcgccg gccggtgctc gcggagctcg ggccagtcga ccacggcctc agggatgatc
     2821 agatccggga ccgcggtcgt cgggccgatg ccccccgcga tgccgaccac gacgacatgg
     2881 tcgacctcac cccggtcgag catccgctgg gcggccgtcg tggccagctc cgtgccgatg
     2941 ccggtggtcg tggccacgac gtcggcctca cccgcgcgcc cgacatggcc ctcgagactg
     3001 cccgatcgtc cccgccgcag cccgaaggcc ttcaccaccg gccgcagctc cgacggcatc
     3061 ggcgccaaca ccccgaaacg catcagcgct tcctagccga ccacgccggc ggcgcggagc
     3121 tcgacgatcc gatcggccag gccggcttcg gccagcagct cgtcggtgtg ctggccgagc
     3181 gccggcgacg ggccgcccac ccctgccggg gtgccctcga aacgggtggg atgacggggc
     3241 tggcggatcg ccccggccac ggggtggtgg ctgtccacca gcaactcgag ggccacggcc
     3301 tgcgggtcct cggccaagtc cgccggcgac acgaggatcc cgtacggcac ccgttcggcc
     3361 tccaagcggc gcgaggcatc ggccacggtg aggccggcgg cggcggccca gcagcgcccg
     3421 acgatctccc tggtcagatc ggcgttctgc tggcgcaggg tggggctggc caggcgggga
     3481 tcgtcatggc cgttcacgcc gaaggcccgg cacatgccga agaagtcagc gtccgaggtg
     3541 ggggtgacca tcccccagcc gtcctcgaag gcgatgggcc gggcgccggc cgagaagctt
     3601 cccggctggg agccgtcacc gtcccgtagg acctcgttgc cggcggcgtc gacccacagg
     3661 aaggcgacga cggcctccag catcgagagg cgcaggtgct gaccgccgcg cccccgctcg
     3721 cgggccagca gggcggcggt gacggcctgg gccgcggtca gggcggtgac cttgtcggcg
     3781 atgacctggc gcacgaaggc cggctcaccg gtgtcgggat cggcctgggc gtgacccatg
     3841 cccgcctggg cctggatcac ggtgtcgtag gccccgcggt cggccttcgg gccgacgtcg
     3901 ccgaaccccg agatcgacac gtagacgagg tcgtcccggc gcaggtcctc gtaccccaca
     3961 ccgagccggt cggccacccc gggccgccag ttctggatca ccacgtcgca gtcggcggcc
     4021 agggcccgca ccacgtcccg acccgcctcg accgagaggt cgacggcgat ggagcgcttg
     4081 ccccggttgc agagctggaa cagggcgctg atcccgtcga tctggacgcc gacgtagcgg
     4141 gcgatgtccc cgatgccgag gcgctcgacc ttgatcacct cggcgccctg gtcggccagc
     4201 atcgtggccg cgtaaggccc ggtggcggcg atggccaggt ccatgacctt gatccctgcc
     4261 agtggcccgc ctgctgcgcc ctgcccgtcg tcccccacgg gcccattctg gccctctccc
     4321 cgcgtcacgg tggtacggcc gcggcccgtc tccaccatcc tcggatcacc acgacggagg
     4381 tgacgccatg ggtcggccag acctcccgcc ggcacgacga cgccagctga ccctcaggcg
     4441 ggcgcgaaga aggtgacgac c
//
LOCUS       DASTFV010000164         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_575620, whole genome shotgun sequence.
ACCESSION   DASTFV010000164 DASTFV010000000
VERSION     DASTFV010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575620"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VFP02_03110"
     CDS             complement(<1..720)
                     /locus_tag="VFP02_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9542042.1"
                     /translation="MTRSFAFADLWELVAEQVPDREAVVCLDRRLTYGDLDERANRLA
                     AHLRANGVGPGDHVGVYLTNGTEYLETMLAAYKLRAVPINVNYRYVEDELRYLFADAG
                     VRAVVHDAEFADRMAAVFDDLPELAVALAVGEDYEAALAAADPAPAFEDRSSDDLYIL
                     YTGGTTGMPKGVVWRQEDAFHSCIGGGDPARLLGAITTPEGIVDRIAPEGSAAVFLPV
                     APLMHAAGQWTSLSWLFAGGKV"
     gene            complement(752..1567)
                     /locus_tag="VFP02_03115"
     CDS             complement(752..1567)
                     /locus_tag="VFP02_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542043.1"
                     /translation="MVAHLQRFAAQTGLPVVNTWGAKGVFPWDSPFHAGTAGLQARDF
                     ELAGLTDKLAVVCIGVDPDELPPGGFGDAQRLDLHPVHLAFAAEGWPEPEGEPPRPPL
                     YTDLAAVIGPAYESEAVPLHPARASRDLGALRPFGGVVVADPGPAGLWVARTFPTTEP
                     GSVLVPGTNAPGTAAAAALLGGLDGSRPALGVTTWPLDPMTEALLDLARSLEVDLTLE
                     AWGGEADLSSPDDRQEATAAAIARSGVDVVPVPVDFRATADLVAVAGEISAWS"
     gene            complement(1571..2041)
                     /locus_tag="VFP02_03120"
     CDS             complement(1571..2041)
                     /locus_tag="VFP02_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542044.1"
                     /translation="MTGRTASDLLAAGLTALGVTRVFGEPVAVPWPGLTTVPVADGVI
                     ADLLADVDGRLDRVGASWHDRVLRVSSRPGALVESVRIDSAESLANALATTLGQEVPD
                     AFALQLDLDLAGPVPDGIEPKAAQTRIEGIRLSPGLAPDLGVLVGPAVLRVLDG"
BASE COUNT          314 a          790 c          852 g          271 t
ORIGIN      
        1 gaccttgccg ccggcgaaca gccaggacag gctcgtccac tgcccggcgg cgtgcatgag
       61 gggggccacc ggcaggaaga cggcggccga cccctccggg gcgatgcggt ccacgatgcc
      121 ctcgggcgtg gtgatggcgc cgagcagacg ggcggggtcc ccgccgccga tgcaggagtg
      181 gaacgcgtcc tcctggcgcc agacgacgcc cttgggcatg ccggtggtgc cgcccgtgta
      241 gaggatgtag aggtcgtcgc tgctgcggtc ctcgaacgcg ggcgcggggt cggcggcggc
      301 cagcgcggcc tcgtagtcct cccccaccgc caacgccaca gccagctcgg gcaggtcgtc
      361 aaagacggcg gccatgcgat ccgcgaactc ggcgtcgtgg accaccgccc ggaccccggc
      421 gtcggcgaac aggtagcgca gctcgtcttc gacgtagcgg tagttcacgt tgatcgggac
      481 ggcccgcagc ttgtaagccg ccagcatcgt ctccaagtac tcggtcccgt tcgtcaggta
      541 gacgccgacg tggtcaccgg ggccgacccc gttggcccgc aagtgggcgg ccagccggtt
      601 ggcccgctcg tcgagatccc cgtaggtcag tcgccggtca aggcagacca ccgcttcccg
      661 gtccggcacc tgctcggcca ccaactccca caggtcggcg aaggcgaacg accgggtcat
      721 ggccggggac cgtagacgcg aggcgcggcg gtcaagacca agcgctgatc tcgccggcga
      781 cggcgaccag gtcggcggtg gcgcggaagt cgacagggac ggggacgacg tcgactccgg
      841 agcgggcgat ggcggcggcg gtggcttcct ggcggtcatc gggcgaggac aggtcggcct
      901 cgccacccca cgcctcgagg gtgaggtcga cctcgagcga ccgggccagg tcgagcagcg
      961 cctccgtcat cgggtcgagt ggccacgtcg tcacgcccag ggcgggccgg gacccgtcga
     1021 ggcccccgag gagggcggcc gcggcggcgg tgcccggggc gttcgtgccc ggcacgagga
     1081 ccgagccggg ctcggtcgtc gggaaggtgc gggccaccca cagcccggcg gggccgggat
     1141 cggccaccac gacgccgccg aagggccgga gggcgccgag atcccgactg gctcgggccg
     1201 ggtgcagcgg cacggcctcg gactcgtagg cggggccgat cacggcggcc aggtcggtgt
     1261 agagcggtgg gcggggtggc tccccctccg gctccggcca gccttcggcg gcgaaggcca
     1321 ggtgcaccgg atggaggtcc aggcgctggg cgtcaccgaa accgcccggg ggcaactcgt
     1381 cggggtccac gccgatgcag accacggcca gcttgtcggt caagccggcc agctcgaagt
     1441 cccgggcctg caggcccgcc gtgccggcgt ggaacgggct gtcccagggg aacacgccct
     1501 tggcccccca ggtgttgacc accgggaggc cggtctgggc ggcgaaccgc tggaggtggg
     1561 cgaccacgac ctagccgtcc aagacccgga gcacggccgg tccgaccagg acaccgagat
     1621 cgggggcgag cccgggcgac aggcggatgc cctcgatccg ggtctgggcc gccttcggct
     1681 cgatcccgtc gggcaccgga ccggccaggt ccaggtccag ttgcagggcg aaggcgtcgg
     1741 ggacctcctg gcccagggtg gtggccaggg cgttggccag gctctcggcg ctgtcgatgc
     1801 ggaccgactc gacgagggcg cccggccggg agctgacccg cagcacccgg tcgtgccacg
     1861 acgcccccac tcggtccagc cgcccgtcga cgtcggccaa caggtcggcg atgaccccgt
     1921 cggcgacggg cacggtcgtc agccccggcc acggcaccgc gacgggctcc ccgaacaccc
     1981 gcgtcacccc gagggccgtg agccccgcgg ccagcaggtc cgaagcggtg cgcccagtca
     2041 tccccgccga gggtaggccc gcttctggcc tctgatctgc cggtcctggc cggtgcatcg
     2101 gaggccagaa cgggggtcag tcggcgaact ggtcccggag ctcgtacttg accactttgc
     2161 cggtggggtt cctcgggagg acgtccatga actcgagctg ctccggcagg gcctgcttgc
     2221 ggaggcc
//
LOCUS       DASTFV010000165         3549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_581083, whole genome shotgun sequence.
ACCESSION   DASTFV010000165 DASTFV010000000
VERSION     DASTFV010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581083"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VFP02_03125"
     CDS             complement(<1..679)
                     /locus_tag="VFP02_03125"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET9542045.1"
                     /translation="MALVKTMTADEAVAGIESGMTIGIGGWASRRKPMTLLKALLRTD
                     VDDLTVVTYGGPDVGILCAAGRVRKVVFAFVSLDTIPLEPHFRAAREAGAIEVEEYDE
                     GMFLLGLQAAAWNVPFLPTRVGLGSDLFRVNPRLTTVTSPYAGPDGEPEELVAMPALP
                     LDVSLLHLNRADERGNAQFLGPDLYMDDLFASAAEATFISCEKVVPTAELTPFHTLRI
                     NRLQVDGV"
     gene            complement(681..1451)
                     /locus_tag="VFP02_03130"
     CDS             complement(681..1451)
                     /locus_tag="VFP02_03130"
                     /inference="COORDINATES: protein motif:HMM:NF005925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HET9542046.1"
                     /translation="MPITSEVDAHGIATVVMDNPPVNALTVAGWFELAGIVRALGDDP
                     AVRVVILAAEGRGYNAGVDIKEMQATEGYDALIGANKGCYAAFAAVYECAVPVIAAVH
                     GYCLGGGIGLVGNADVIIAAEDATFGLPEVDRGALGAATHLARLVPQHKMRAMVYTST
                     VATAAELHHYGSVLKVVPRDELMAAAREVAETIAAKSPTVIRAAKESLNGIDPVDVKR
                     SYRFEQGFTFELNLAGVADEARDAFVERGENADFGGED"
     gene            1546..2301
                     /locus_tag="VFP02_03135"
     CDS             1546..2301
                     /locus_tag="VFP02_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9542047.1"
                     /translation="MSAATDLDGRVALVSGGARGLGRGVADGLLAAGARVAVCGRSQP
                     DELPAGARFFGADVRDAGAVDGLVAAVAEHYGRLDIAVNNAGGSPVAEAATAAPAFST
                     AIVTLNLLAPLLVSQAVNRVMQGQDDGGAIVNIGSLTGPSPSPGTAAYGAAKAGLVDL
                     SRTLAVEWAPKVRVNCVSGDLIATDAAADDDADAIAAVEATVPLGRLGTVSDMAGVVC
                     YLASPASAYVTGANLVIHGGGRRPTYLGAIAGD"
     gene            2455..>3549
                     /locus_tag="VFP02_03140"
     CDS             2455..>3549
                     /locus_tag="VFP02_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542048.1"
                     /translation="MSDLVIVRRILTTLTAVLVGASALAVVPVALPSAQGEGEPTLAT
                     SVAEGLTGTSIQVSGTGCYLPDGITGADGVLFQLIAPDGSAAASGTLPVERDGTWDAP
                     FVVPNGVPAGTYGVKGTCIAPMYEDLGVVTAEPFTVTGAGAPVPEREPATPRFPNEIE
                     AYPTYDGQSTCSPAPKPGMIAYRDMVMRAYPASSSYGISRDCSVGGTSEHKEGRAWDW
                     ANDATTAAGRRRVADFFRWLFRTDSYGHRHAMARRLGVMYIIWNRQIFRMYRANEGWT
                     PYTGSSPHTDHVHVSLTRRGGNKRTSFWTMRLSGPTDPPPPTDPPPRPRRAPAEFEQT
                     ARAVDGTFEHAQVGDFDGNGKNDILWYGEGA"
BASE COUNT          514 a         1311 c         1234 g          490 t
ORIGIN      
        1 ccacgccgtc cacctgcagc cggttgatgc gcagggtgtg gaagggggtg agctcggccg
       61 tcggcacgac tttctcacag gagatgaagg tggcctcggc ggccgatgcg aacaggtcgt
      121 ccatgtagag gtcgggcccg aggaactggg cgttgcctcg ctcgtcggcc cggttcaggt
      181 ggagcaacga cacgtccagg ggcagggcgg gcatggcgac cagctcctcc ggctcgccgt
      241 cgggccccgc gtagggcgag gtcacggtcg tcagccgggg attcacccgg aagaggtccg
      301 agccgaggcc tacccgggtg gggaggaacg ggacgttcca ggccgcggcc tggagcccca
      361 gcaggaacat gccctcgtcg tactcctcga cctcgatggc ccccgcctcg cgggcagccc
      421 ggaagtgggg ctcgagagga atcgtgtcga gcgagacgaa ggcgaacacc accttgcgga
      481 cccggccggc ggcacagagg atgccgacgt cggggcctcc gtacgtcacc accgtcaggt
      541 cgtcgacgtc ggtccgcagc agggccttga gcagggtcat cggcttgcgc cgggacgccc
      601 agcccccgat gccgatggtc atgccgctct cgatcccggc gacggcctcg tcggccgtca
      661 tggtcttgac caaagccacg tcagtcctcg ccgccgaagt cggcgttctc gccccgctcg
      721 acgaaggcgt cgcgggcctc gtcggccacg ccggccaggt tcagctcgaa ggtgaagccc
      781 tgttcgaagc ggtacgagcg cttcacgtcg accgggtcga tcccgttcag ggactccttg
      841 gccgcccgga tcaccgtcgg gctcttcgcc gcgatcgtct cggccacctc gcgggcggcg
      901 gccataagct cgtcccgggg cacgaccttc aggaccgatc cgtagtggtg cagctcggcc
      961 gcggtcgcca cggtcgacgt gtagaccatc gcccgcatct tgtgctgggg gaccaggcgg
     1021 gccaggtgcg tggccgcgcc gagggcgccc cggtccactt caggcagccc gaaggtggcg
     1081 tcctcggcgg cgatgatcac gtcggcgttg ccgaccaggc cgatgccccc gccgaggcag
     1141 tagccgtgga cggcggcgat gacgggcacg gcgcactcgt agaccgcggc gaaggcggcg
     1201 taacagccct tgttggcccc gatcagggcg tcgtagccct cggtggcctg catctccttg
     1261 atgtcgaccc cggcgttgta gccccgaccc tcggcggcca ggatcaccac ccggacggca
     1321 gggtcgtcgc ccagcgcgcg gacgatgccg gccagctcga accagcccgc caccgtcagg
     1381 gcgttgaccg gtgggttgtc catgaccacg gtggcgatcc cgtgggcgtc gacctcggac
     1441 gtgatgggca tgcgcggcgg accgtaaacg atgcgacccc tgccgggcga acgggtcggg
     1501 gcttcctccg tcgtgccggc cggttcgccc tacgctcgcc cgaggatgag cgcagcaacc
     1561 gatctcgacg gccgggtcgc cctggtgagc ggcggggccc ggggcctcgg acggggtgtc
     1621 gccgacggac tcctcgccgc cggcgcccgt gtcgccgtct gtggccgcag ccagcccgac
     1681 gagctgcccg cgggggcccg gttcttcggc gccgacgtcc gggacgccgg ggcggtggac
     1741 ggcctggtcg ccgcggtggc cgagcactac ggccgcctcg acatcgccgt gaacaacgcc
     1801 ggcggctcgc ccgtcgccga ggcggctacg gccgctccgg cgttctccac ggccatcgtc
     1861 acgctcaacc tcctggcgcc actgctcgtg agccaggccg tcaaccgggt catgcagggc
     1921 caggacgacg gcggggccat cgtgaacatc ggctcgctca ctggcccgag cccgtcaccg
     1981 gggaccgcgg cctacggggc ggcgaaagcc ggcctggtcg acctcagccg gacgttggcc
     2041 gtcgaatggg cgcccaaggt ccgcgtgaac tgtgtgtcgg gcgacctcat cgccaccgac
     2101 gcggccgcgg atgacgacgc cgacgccatc gccgcggtcg aggccacggt gcccctcggt
     2161 cgactcggca cggtgagcga catggccgga gtcgtgtgct acctcgcgtc gcccgcgtcg
     2221 gcctacgtga ccggggccaa cctggtgatc cacggcggcg gccgccgccc gacctacctt
     2281 ggagccatcg ccggcgactg agcccggcgg cccacgaggg ttggcgcgcc gctgaccccg
     2341 tcgcctacat ttctgtaacc agttcgcaaa gaaactgcaa tcagatctgc gcggtggaag
     2401 ggaagcccac cgaaaggcaa cgtgaatgcg cagagtgttc acagtccgca tgcaatgtcc
     2461 gaccttgtga tcgtgcgccg aattctcacc accctgaccg ccgtgctggt cggggcgtcc
     2521 gccttggccg tcgtgccggt cgccctcccc tcggcgcagg gcgaggggga gccgaccctg
     2581 gcgacctccg tcgccgaggg cctcaccggc acctccatcc aggtctcggg caccggctgc
     2641 tacctgcccg acgggatcac gggcgccgac ggcgtgctgt tccagctcat cgcccccgac
     2701 ggcagcgccg ccgcctcggg caccctcccg gtcgagcggg acgggacctg ggacgctccc
     2761 ttcgtcgtcc ccaacggcgt ccccgccggc acctacggcg tcaagggcac ctgcatcgcc
     2821 cccatgtacg aggacctggg cgtggtcacc gccgagccct tcacggtcac cggggccggc
     2881 gcccccgtgc ccgagcggga gccggccacg cctcgcttcc ccaacgagat cgaggcctac
     2941 ccgacctacg acggccagag cacctgcagc ccggcgccca agccgggcat gatcgcctac
     3001 cgggacatgg tcatgcgggc ctatccggcg tcgtcgagct acggcatcag ccgggactgc
     3061 tccgtcggcg ggaccagcga gcacaaggaa gggcgggcct gggactgggc caatgacgcc
     3121 accacggccg cggggcgccg ccgggtcgcc gacttcttcc gttggctgtt ccgcaccgac
     3181 agctacggcc accgccacgc catggcccgt cgcctcggcg tcatgtacat catctggaac
     3241 cgccagatct tccgcatgta ccgggccaac gaggggtgga cgccgtacac cgggtccagc
     3301 ccccacaccg accacgtcca cgtcagcctg acccgccggg gcggcaacaa gcggaccagc
     3361 ttctggacca tgcggctctc agggcccacg gatccgccgc cgccgaccga tcccccgccc
     3421 cgtcccaggc gggccccggc cgagttcgag cagaccgcgc gggccgtcga cggtacgttc
     3481 gagcacgccc aggtcggcga cttcgacggc aacggcaaga acgacatcct ctggtacggc
     3541 gaaggggcc
//
LOCUS       DASTFV010000166         3511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_583287, whole genome shotgun sequence.
ACCESSION   DASTFV010000166 DASTFV010000000
VERSION     DASTFV010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583287"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..579
                     /locus_tag="VFP02_03145"
     CDS             151..579
                     /locus_tag="VFP02_03145"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9542049.1"
                     /translation="MTAARFNHVGQCVTDLDRSRRFYVEVFGFEVARELHPPDQGSDR
                     LLRLPAAVGMTAVYLKRDDFILELLHFADAGVVAGEERVVNQPGLTHVSLSVDDLDAS
                     LAAVEAHGGEVLTDTNIGVAVFVKDPDGQLLELLTNWDKP"
     gene            complement(765..1214)
                     /locus_tag="VFP02_03150"
     CDS             complement(765..1214)
                     /locus_tag="VFP02_03150"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET9542050.1"
                     /translation="MTLNPGDAAPDFALPDQTGTTRQLSDLLADGPVVLFWYPAAMTT
                     GCTKEACHFRDIAAEFEAVGAQRVGISADAPEKQAQFDEKHDLGYPLLSDQDKAVAKA
                     YGVKRSLLPMNKRATFVIGTDGKIVDVITSETNMNAHADQALQVLRA"
     gene            1248..1703
                     /locus_tag="VFP02_03155"
     CDS             1248..1703
                     /locus_tag="VFP02_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET9542051.1"
                     /translation="MPTVFERPADLLDGVGTDLGTTAWLEITQERVDTFADATGDHQW
                     IHVDPERAKDGPFGGTIAHGYLTLSLVNTFLPDLLEVRNVSLGVNYGTDKTRFPSAVP
                     VGSRIRGRAELTGAEEVKGGVQARVTVTVEIEDSDRPACVVETISRFLD"
     gene            1703..2434
                     /locus_tag="VFP02_03160"
     CDS             1703..2434
                     /locus_tag="VFP02_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9542052.1"
                     /translation="MAEQPARRDEPLPGSLRETRDEVPEAIPAATVILLRDGANGLET
                     LMLRRNSKLEFAGGMWVFPGGRVDPEDYPPDAHDDVEAAVRVAAAREAAEEAGLACDP
                     DAMVPFAHWTPPMVAIKRYATWFFVAEAPTGAVTIDDGEIHESIWARPEDTLARRDAG
                     EVELAPPTWVTLDRLTKSATVAEALDEARNAPVEAFVTAVGKDGDDLVALWHGDAGYE
                     TGDPTVPGPRHRLVMSADTWRYHRD"
     gene            2560..>3511
                     /locus_tag="VFP02_03165"
     CDS             2560..>3511
                     /locus_tag="VFP02_03165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET9542053.1"
                     /translation="MPDRRQPTTTSSFGVSGRSNHDASDFYARFTPPELSDDSTVEAP
                     LEVDVIIPGDARAMTRVADRSVALVVTSPPYFAGKAYEQDLGEGHIPATYLEYLQMLE
                     DVFTECARVLEPGGRIAVNVANLGRKPYRSLSADVIAILQDRLRLLLRGEVVWIKARG
                     AAGSFAWGSFRQPSNPVLRDLSERVIVASKGRFDRGRPADSTLTKDEFMEATTDVWEL
                     PTESARRVGHPAPFPVELPQRLIELYTYPGDLVLDPFMGSGSTAVAAVRTGRHYVGYD
                     TDVDYVDAASARVDAERITLTATGHDPAPTAGGESARVLAA"
BASE COUNT          499 a         1246 c         1231 g          535 t
ORIGIN      
        1 ggcgtgctgg ccgagcgtgc tccggtagtc gccgtcgatg atggtgtcgg gcttgtcggc
       61 gaaggcgtcc aggccgggga ggaacagccg gtggtacacg agctccttca tgcggggcag
      121 ggtaggtgac ccaccactag gttgccgatc gtgaccgcgg cccggttcaa ccacgtgggg
      181 cagtgcgtca ccgacctcga tcgctcgcgt cggttctacg tggaggtctt cggcttcgag
      241 gtcgcccgcg agctccaccc tcccgaccaa ggctcggacc gtctgctgcg gctgccggcg
      301 gcggtcggga tgacggccgt ctacctgaag cgggacgact tcatcctcga gctgctccac
      361 ttcgccgacg ccggggtggt ggcgggggag gagcgggtgg tgaaccagcc cggcctgacc
      421 cacgtgtccc tcagcgtcga cgacctcgac gcgtcgctgg cggccgtgga ggcccacggc
      481 ggcgaggtgt tgaccgacac caacatcggc gtggccgtct tcgtcaagga ccctgacggc
      541 cagctcctcg agctgttgac caactgggac aagccctagc agagcgaacc ttcggcgtca
      601 ggcaccgccg atgtcgagtt ctgccgaagg ttggctggag atgcgaactt tcggcgtgag
      661 gcaccgccga tgtcgagttt tgccgaaggt tggcgggaga tgcgaacttt cggcgtgagg
      721 caccgtcgat gtcgagttct gccgaaggtt ggcgggtagc gcggctacgc gcggaggact
      781 tggagggcct ggtcggcgtg ggcgttcatg ttggtctcgc tggtgatgac gtcgacgatc
      841 ttgccgtcgg tgccgatgac gaaggtggcc cgcttgttca tcggcagcag gctgcgcttg
      901 acgccgtagg ccttggcgac ggccttgtcc tggtcggaga ggagcgggta gccgaggtcg
      961 tgcttctcgt cgaactgggc ctgcttctcg ggagcgtcgg cgctgatgcc cactcgctgg
     1021 gcgccgaccg cctcgaactc ggcggcgatg tcacggaagt ggcacgcctc cttggtgcag
     1081 ccggtggtca tcgccgccgg gtaccagaac agcacgacgg gcccgtcggc cagcagatcg
     1141 ctgagctggc gggtcgtgcc cgtctggtcg ggcagggcga agtccggggc ggcatcgcca
     1201 gggttgaggg tcatcggggt cctccgtcgg tccgtagagt cgaccttatg ccgacggtgt
     1261 tcgaacgccc tgccgatctg ctcgacgggg tcggcaccga tctcggcacg acggcctggc
     1321 tggagatcac ccaggagcga gtcgacacct tcgccgacgc caccggcgac caccagtgga
     1381 tccacgtcga cccggaacgg gccaaggacg ggccgttcgg cggcaccatc gcccacggct
     1441 acctcaccct ctcgctggtc aacaccttcc tccccgacct gctcgaggtg cgcaacgtct
     1501 ccctgggcgt caactacggg accgacaaga cccgcttccc ctcggcggtg ccggtgggca
     1561 gccgcatccg agggcgggct gagctgaccg gcgccgagga ggtcaagggc ggggtccagg
     1621 cccgggtcac ggtgaccgtc gagatcgagg acagcgaccg cccggcctgt gtggtcgaga
     1681 ccatctcccg gttcctggac tgatggccga gcagccggcc cggcgggacg agcccctccc
     1741 tggatcctta cgggagacca gggacgaggt ccccgaggcc atccccgcgg ccaccgtcat
     1801 cctcctgcgg gacggggcca acgggctgga gaccctgatg ctgcgccgca actccaagct
     1861 ggagttcgcc ggcggcatgt gggtgttccc cgggggtcgg gtcgacccgg aggactaccc
     1921 gcccgacgcc cacgacgacg tcgaggccgc ggtccgggtc gcggccgccc gtgaggccgc
     1981 ggaggaggcc ggtctggcct gtgaccccga cgccatggtc cccttcgccc actggacacc
     2041 gccgatggtt gccatcaagc gctacgccac ctggttcttc gtcgccgagg ccccgaccgg
     2101 cgcggtgacc atcgacgacg gcgagatcca cgagtcgatc tgggcccgcc ccgaggacac
     2161 gctggcccgg cgtgacgccg gagaggtcga actggccccg cccacctggg tcaccctcga
     2221 ccgcctgacc aagtccgcca ccgtggccga ggccctggac gaagcccgga acgcccccgt
     2281 ggaggccttc gtcaccgccg tcggcaagga cggcgacgac ctcgtggccc tctggcacgg
     2341 cgacgccggc tacgagaccg gcgaccccac cgtccccggc ccccgccacc gcctcgtcat
     2401 gtccgccgac acctggcgct accaccgcga ctgaacagat gggggcggag tgtcactggg
     2461 gttctgcatg atgtcggcat gggcgcgcat ccagggacga cgaccggccg ggccgtagca
     2521 tcggggatcc ggcgccagcc ccggcccggg aggagagccg tgcccgaccg tcgccagccc
     2581 acgaccacgt cctccttcgg cgtgtcgggc cggtccaacc acgacgccag cgacttctac
     2641 gcccgcttca ccccgcccga gctgtccgac gactcgacgg tggaggcgcc gctcgaggtc
     2701 gacgtgatca tccccggcga cgcccgggcg atgacccggg tggccgaccg ctccgtggcg
     2761 ctggtcgtca cctcgccccc gtacttcgcg gggaaggcct acgagcagga cctgggcgag
     2821 gggcacatcc ccgccaccta cctcgagtac ctccagatgc tggaggacgt cttcaccgag
     2881 tgcgcccgtg tgctcgagcc cggcggccgc atcgcggtca acgtggccaa cctcgggcgc
     2941 aagccctacc gctcgctctc ggccgacgtc atcgccatcc ttcaggaccg cctgcggctg
     3001 ctgctgcggg gcgaggtggt ctggatcaag gcccggggcg cggccggttc gttcgcctgg
     3061 ggctcgttcc gccaaccgtc caaccctgtg ttgcgggacc tgtccgagcg ggtgatcgtc
     3121 gccagcaagg gccgcttcga ccggggtcgc ccggcggaca gcacgctgac caaggacgag
     3181 ttcatggagg cgaccaccga cgtgtgggag ctgcccaccg agtcggcccg ccgcgtcgga
     3241 cacccggcgc cgttcccggt ggagctgccc cagcgcctca tcgagctcta cacgtatccc
     3301 ggtgacctcg tgctcgaccc gttcatgggc tcgggctcga ccgcggttgc cgcggtgcgc
     3361 accggccgcc actacgtcgg ctacgacacc gacgtcgact acgtcgacgc ggcctcggcc
     3421 cgggtggatg ccgagcgcat caccttgacc gccaccggtc acgatcccgc cccgacggcc
     3481 ggcggcgagt cggctcgcgt gctggccgcc a
//
LOCUS       DASTFV010000167         3183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_588971, whole genome shotgun sequence.
ACCESSION   DASTFV010000167 DASTFV010000000
VERSION     DASTFV010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588971"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..965)
                     /locus_tag="VFP02_03170"
     CDS             complement(3..965)
                     /locus_tag="VFP02_03170"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET9542054.1"
                     /translation="MLVIQRPTVEPVSEADGNRQKFSIAPLEPGFGHTLGNSLRRTLL
                     SSIPGAAITQVRFDDALHEFDTISGITEDVTDILLNLKDVVVVSHSDEPVEVRLDVRG
                     ATDVTGADLETNPDIEILNKELHIATLNTKARLALDLTIEKGRGYRSGDRDAEETTIG
                     IIPLDAIYSPVRRVTFEVEATRVEQETAYDALILDIETDGSISPREALASAGGTLRAL
                     GALVDEVSDEPQGLELTEAGPQAAGSPDLDLPIEDLDLSERPRNCLKRAQVNTVGELL
                     EKSEDDLLAITNFGQKSLDEVIAKLDERGLTLKGGPATIIPAPE"
     gene            complement(1013..1630)
                     /gene="rpsD"
                     /locus_tag="VFP02_03175"
     CDS             complement(1013..1630)
                     /gene="rpsD"
                     /locus_tag="VFP02_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012010904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET9542055.1"
                     /translation="MSRYTGPRARVSRRFGVNIWGTKGETIALDRRPYPPGEHGRDRR
                     RGNQSDYLLQLTEKQKARFTYGLTEKQFRRTYDEANRRSGVTGENMLRYLELRLDNVV
                     YRAGWAATRPQARQLVNHGHIEVNGKRVDIPSYRVRKGEVISLSTKAKALIVVTWNRD
                     VLDRVPPPWLETGAEGAQVTVRELPLREHIDVPVREQLIVELYSK"
     gene            complement(1630..2028)
                     /gene="rpsK"
                     /locus_tag="VFP02_03180"
     CDS             complement(1630..2028)
                     /gene="rpsK"
                     /locus_tag="VFP02_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET9542056.1"
                     /translation="MAKPAAGGRRPKKRERKNIPHGIAHIKSSFNNTIITVTDRQGNV
                     LSWASAGNVGFKGSRKSTPYAAQMAAETATKRAMEHGVRKVDVEVKGPGSGRETAIRT
                     IQQMGIEVTGIKDVTPVPHNGCRPPKRRRV"
     gene            complement(2028..2405)
                     /gene="rpsM"
                     /locus_tag="VFP02_03185"
     CDS             complement(2028..2405)
                     /gene="rpsM"
                     /locus_tag="VFP02_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET9542057.1"
                     /translation="MARIAGVDIPREKRTVIALTYIFGIGDTLASEVCTNTGVDPSRR
                     VRDLTDEEVSQLRVYIDENLQVEGDLRRDIQQDIKRKMEIGAYQGIRHRRGLPVRGQR
                     THTNARTRKGPRKTVAGKKKVGK"
     gene            complement(2410..2523)
                     /gene="rpmJ"
                     /locus_tag="VFP02_03190"
     CDS             complement(2410..2523)
                     /gene="rpmJ"
                     /locus_tag="VFP02_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET9542058.1"
                     /translation="MKVRPSVKKMCEKCKVIRRHGRVRVICTNPRHKQRQG"
     gene            complement(2534..2758)
                     /gene="infA"
                     /locus_tag="VFP02_03195"
     CDS             complement(2534..2758)
                     /gene="infA"
                     /locus_tag="VFP02_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET9542059.1"
                     /translation="MAKPKEDAIVLEGTVLESLPNAMFKVELENGHELLAHISGKMRM
                     HYIRILPGDRVQVELTPYDLKRGRITYRYK"
     gene            complement(2913..3095)
                     /locus_tag="VFP02_03200"
     CDS             complement(2913..3095)
                     /locus_tag="VFP02_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET9542060.1"
                     /translation="MSDSSASTSSEPSVVDSNISSSGVGGTLARTGADPGLLLLLGGG
                     LVVTGVLVLRYRPREA"
BASE COUNT          433 a          985 c         1189 g          576 t
ORIGIN      
        1 gactactccg gcgcggggat gatggtggcg gggccaccct tgagggtcag gccccgctcg
       61 tccagcttgg cgatgacctc gtcgagggac ttctggccga agttggtgat ggccaggagg
      121 tcgtcctcgg acttctccag cagctcaccc accgtgttga cctgggcccg cttgaggcag
      181 ttccggggcc gctcggagag gtcgaggtcc tcgatgggca ggtccaggtc cggcgaaccg
      241 gcggcctggg ggccggcctc ggtcagctcc aggccctggg gctcgtcgct gacctcgtcg
      301 accagggccc cgagcgcccg cagggtgccg ccggcggagg ccagggcctc gcgggggctg
      361 atggacccgt cagtctcgat gtcgaggatg agggcgtcgt acgccgtctc ctgctcgacc
      421 cgggtggcct ccacctcgaa ggtgacgcgg cgcacgggcg agtagatggc gtccaggggg
      481 atgatgccga tggtggtctc ctcggcgtcc cggtccccag agcggtagcc ccggcccttc
      541 tcgatggtca ggtccagcgc gaggcgggcc ttcgtgttga gggtggcgat gtggagctcc
      601 ttgttcagga tctcgatgtc ggggttggtc tcgaggtcgg ccccggtgac gtcggtcgcc
      661 ccgcggacgt cgaggcggac ctcgacgggc tcgtcactgt gggagaccac gacgacgtcc
      721 ttgaggttca acaggatgtc ggtgacgtcc tcggtgatgc ccgagatcgt gtcgaactcg
      781 tgcagggcgt cgtcgaagcg gacctgggtg atggccgctc cggggatcga cgacagcagg
      841 gtccggcgca gggagttgcc cagggtgtgg ccgaagccgg gctccaacgg agcgatggag
      901 aacttctgtc ggttgccgtc ggcctcgctg acgggctcga cggtcggtcg ctgaatgacc
      961 agcatggcgt ttcctctcgg acctttttga acgggcgccc cggggcaggg gcttacttgc
     1021 tgtagagctc gacgatcagc tgttcccgca cgggcacgtc gatgtgctca cgcaggggca
     1081 gttcgcgcac ggtgacctgg gcaccctcgg cgccggtctc gagccagggc ggcgggaccc
     1141 ggtcgaggac gtcgcggttc caggtgacga cgatgagggc tttggccttg gtcgacaggg
     1201 agatgacctc gcccttgcgg acccggtagc tcgggatgtc gacccgcttg ccgttgacct
     1261 cgatgtggcc gtggttcacc agctggcggg cctgggggcg ggtggcggcc cagccggcgc
     1321 ggtagacgac gttgtcgagg cggagctcga ggtagcgcag catgttctcg ccggtcacgc
     1381 ccgagcgccg gttggcctcg tcgtaggtgc ggcggaactg cttctcggtc aggccgtagg
     1441 tgaaccgggc cttctgcttc tcggtgagct gcagcaggta gtcggactgg ttgccgcggc
     1501 gacggtcacg accgtgctcg ccgggcgggt aggggcgacg atccagggcg atggtctcgc
     1561 ccttggtgcc ccagatgttg acgccgaagc gccgcgagac gcgggcgcgt ggaccggtgt
     1621 agcgggacat cagaccctcc gccgcttggg ggggcggcag ccgttgtggg gcacgggggt
     1681 gacgtccttg atgccggtca cctcgatgcc catctgctgg atggtgcgga tggcggtctc
     1741 gcggcccgag cccgggccct tgacctcgac atcgaccttg cgcacgccgt gctccatggc
     1801 ccgcttggtg gcggtctcgg ccgccatctg ggcggcgtac ggcgtggact tgcgggagcc
     1861 cttgaagccg acgttgccgg cggaggccca cgacagcacg ttcccctggc gatcggtgac
     1921 cgtgatgatc gtgttgttga aggagctctt gatgtgagcg atcccgtggg ggatgttctt
     1981 gcgttcgcgc ttcttggggc gacgaccgcc ggcggcgggc ttggccatta cttgcctacc
     2041 ttcttcttgc cggccacggt cttgcgtggg cccttgcggg tgcgggcgtt ggtgtgggtg
     2101 cgctgacccc ggacgggcag gccacgacgg tggcggatgc cctggtaggc cccgatctcc
     2161 atcttgcgct tgatgtcctg ctggatgtcc cggcgcaggt caccctcgac ctgcaggttc
     2221 tcgtcgatgt agacccggag ctggctgacc tcttcgtcgg tcaggtctcg cacccggcgg
     2281 ctcgggtcga ccccggtgtt ggtgcagacc tcggaggcga gggtgtcgcc gatgccgaag
     2341 atgtacgtga gggcgatgac ggtccgcttc tcgcgaggga tgtcgacgcc ggctatacgg
     2401 gccattccgt tacccctgcc gctgcttgtg tcgtgggttg gtgcagatga cgcggacccg
     2461 accgtgacgg cggatgacct tgcacttctc gcacatcttc ttgacgctcg gtcgtacctt
     2521 caacagatcc tcgtcacttg taccgatagg tgatgcgacc tcgtttgagg tcgtaggggg
     2581 tcagctcgac ctggacgcgg tccccgggca ggatccggat gtagtgcatc cgcatcttcc
     2641 cggagatgtg ggccaacaac tcgtggccat tctccaactc caccttgaac atggcattgg
     2701 gcaacgactc gagcaccgtg ccctcgagca cgatcgcgtc ttccttcggc ttcgccagga
     2761 caatctccga acaactagtg gtgagcggca cgccgggccg cgcgcaagaa cgcgctggac
     2821 ccggggccga cgggcaaggc tagcggcgcg ccggggccgc agccaacctc cgccctagca
     2881 caagggcccg ggcggggcgg ggatcgccgg gctcaggctt cgcgggggcg gtaccgcagg
     2941 acgaggacac cggtgaccac gaggccgcca ccgaggagca ggagcagccc cggatcggcg
     3001 ccggtgcggg ccagggtccc accgacgccg ctggacgaga tgttgctgtc gacgacactc
     3061 ggctccgacg acgtggaggc gctggagtcg ctcacgctgg gcgacgtgga cgaggtgctg
     3121 accgacgtcg acgagacggt cgagctcgtg tcggtggacg tggaggagat ggacgaggtg
     3181 gac
//
LOCUS       DASTFV010000168         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_595118, whole genome shotgun sequence.
ACCESSION   DASTFV010000168 DASTFV010000000
VERSION     DASTFV010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595118"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1889
                     /locus_tag="VFP02_03205"
     CDS             <1..1889
                     /locus_tag="VFP02_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542061.1"
                     /translation="SMYFPPEELYEGRFFQPLMPISGFENAAPMILGGMMGASIDFAV
                     DSGGYLVESNLGRTVMFPGDDTTLVGYRASAAVARYSRVLAAEMYGEHRAYGYVYGGS
                     GGAYKTIGCIENTVDVWDGAVPFVHGSPLSLPNLFTVQAHAIRILHDVFPQIVDAVEV
                     GGSGDPWTGLTVEQRDALAEVTRMGFPLEVWFDVDRVAAGYTGVFCSLIDQVIGFDPD
                     YFDLFWSEPGYLGADPPASLAAARVQHDTTISQVVLQSEATELGLPMSMSAMFGDSEH
                     DMPAALRLADLPEGNLQGASLILNSGDGKGAMLHIAQVIGDLVMTGFGENNFDAMRRF
                     KAGDEVRVDNSIYLASQTYHRHQVPDIEEYPTFAQFCSGGEPIYPQRPEIIGMRFARG
                     GGGSIQSGRFTGKMIVVEALMDETCSPWQADWYRSRVQEALGDRLDDHYRVWFIEHAM
                     HTSPTVLPDDARPVRTTRAVSYAGVLQQALRDLSAWVENGRVPPASTSYEIVDGQVVV
                     PDTAAERKSIQAVVRVAANGRSRAEVATGEDVEFTAEVEVPAGTGTIVTAEWDFEGAG
                     DFPRPTEGVDGSATHLRLSTTYAFREPGTYFPALRVASQRQGAVDSPFARVLNLGRVR
                     VVVS"
BASE COUNT          300 a          741 c          706 g          305 t
ORIGIN      
        1 tctcgatgta cttcccgccc gaggagctct acgaggggcg gttcttccag cccctgatgc
       61 cgatctcggg gttcgagaac gcggcgccca tgatcctggg cggcatgatg ggcgcgtcca
      121 tcgacttcgc ggtcgacagc ggtggctacc tcgtcgagtc gaacctcggc cgcacggtca
      181 tgttccccgg cgacgacacc acgctggtcg gctaccgggc cagcgccgcg gtggcccggt
      241 actcccgggt gctcgccgcg gagatgtacg gcgagcaccg cgcctacggc tacgtgtacg
      301 gaggcagcgg cggcgcctac aagaccatcg gctgcatcga gaacaccgtc gacgtgtggg
      361 acggggcggt gcccttcgtc cacggctcgc cgctgagcct gcccaacctg ttcaccgtcc
      421 aggcccacgc catccggatc ctgcacgacg tgttccccca gatcgtcgac gccgtcgagg
      481 tgggcggcag tggcgacccc tggaccggcc tgaccgtcga gcagcgggac gccctggccg
      541 aggtgacccg gatggggttc cctctcgagg tctggttcga cgtcgaccgc gtcgccgccg
      601 ggtacaccgg cgtgttctgc agcctgatcg accaggtcat cggcttcgac cccgactact
      661 tcgacctgtt ctggtccgag cccggctatc tcggggccga tccccccgcc tcgctggccg
      721 ccgcccgcgt ccagcacgac accaccatca gccaggtcgt gctccagtcc gaggcgaccg
      781 agctcggcct gccgatgtcg atgtcggcga tgttcggcga cagcgagcac gacatgccgg
      841 ccgcgctgcg tctggccgac ctgcccgaag gcaacctcca gggtgcgtcc ctcatcctca
      901 acagcgggga cggcaagggc gcgatgctgc acatcgccca ggtgatcggc gacctggtca
      961 tgaccggctt cggggagaac aacttcgacg ccatgcgccg gttcaaggcg ggcgacgagg
     1021 tgcgggtgga caactcgatc tacctcgcct cgcagaccta ccaccgccac caggtgccgg
     1081 acatcgagga gtacccgacc ttcgcccagt tctgctccgg cggcgagccc atctaccccc
     1141 agcgtcccga gatcatcggc atgcggttcg cgcggggcgg cgggggctcg atccagagcg
     1201 gtcgcttcac cgggaagatg atcgtggtcg aggccctgat ggacgagacc tgctcgccgt
     1261 ggcaggccga ctggtaccgg tcccgcgtcc aggaggcgct gggcgatcgc ctcgacgacc
     1321 actaccgggt ctggttcatc gagcacgcca tgcacacctc gcccaccgtt ctgccggatg
     1381 acgcgcgtcc ggtgcggacc acgcgggccg tctcctacgc cggcgtgctc caacaggccc
     1441 tccgcgacct gagcgcgtgg gtggagaacg gcagggtccc gccggcctcc accagctatg
     1501 agatcgtgga cggtcaggtg gtcgtgcccg acaccgcggc ggagcgcaag agcatccagg
     1561 cggtggtcag ggtggcggcc aacggtcggt cccgggccga ggtcgccacc ggcgaggacg
     1621 tcgagttcac cgccgaggtc gaggtgccgg cggggacggg caccatcgtg accgccgagt
     1681 gggacttcga gggggccggc gacttccccc gtccgacgga gggcgtcgac gggtcggcga
     1741 cccacctgcg gctgtcgacg acctacgcct tccgcgagcc gggcacctac ttcccggcgc
     1801 tgcgggtcgc cagccagcgc caaggcgccg tcgactcacc cttcgcccgt gtcctgaacc
     1861 tcggccgggt ccgggtggtc gtcagctaga cgatggggga ccgcgtggcc gctatcgcgg
     1921 ccgtcggcct gctgtgcctg gccggctgta gcggggacaa cggggacgac gccgcacccg
     1981 cggagaccgt ggaggccgtg acgacgatga ccacgacacc ccctccggcg agcagccggt
     2041 ccctggccac ca
//
LOCUS       DASTFV010000169         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_598008, whole genome shotgun sequence.
ACCESSION   DASTFV010000169 DASTFV010000000
VERSION     DASTFV010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598008"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..>2350
                     /gene="hrpB"
                     /locus_tag="VFP02_03210"
     CDS             23..>2350
                     /gene="hrpB"
                     /locus_tag="VFP02_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HET9542062.1"
                     /translation="MESAVADVRDALATVGTAVLQAEPGAGKTTVVPLRLLDEAWRGD
                     GRIVVLEPRRLATRAAARRMADLLGERVGETVGYRTRDERHVGPATRIEVVTEGILTR
                     RLQADPSLPGTALVVFDEVHERHLQTDLALALALDAREGLRPDLRLLAMSATLEATRL
                     AVLLGGSAGPAPVVTSPGRTHPVDIRWRPPGPRVRPFDAVAAVVHEAVRDEGGDVLVF
                     LPGAAAIRRVGGLLADLPRDVDVRPLHGSLRPEEQDLALAPSASGRRRVVLATDIAES
                     SLTVDGVRVVVDAGEVRRPRHDTRSGLSRLHTTAASRASAVQRAGRAGRLGPGVAYRL
                     WSAGEHAARRPFAPPEIAAADLAGLALELAVWGATADALAFLDPPPPTALADATELLG
                     RLGAIDDAGRPTPTGRTMVDLPLHPRLARMVTDERSALAVVLATLLEERDPLRGRPEE
                     LDADVTERIRLVTDPRRGHPRLDDGARRTARRRAQELARRAGIGFGEVDPSRAGPVLA
                     LAYPDRIAQARGRGRFQLRSGRAVALAPHDPLAAEAFIVVADLAPPAPREVDDRVRLA
                     AALDEAEVEAVAGTEVRETTELAWNARDELEQRAERRLDALVLDRAVGPAPAGAPTTA
                     ALLDRVRDDGLGLLGWTTAARTLQERAGFARRFLGDPWPDLNDEALVAGMAEWLAPLL
                     GRATSRADLRRVDVLAVLRGRLGHRLHELDRVVPARVTIASGRTVAGDYDHDPPSIRV
                     RAQELYGTAIHPTVADGRVPLTVEVLSPAGRPVQVT"
BASE COUNT          246 a          952 c          872 g          280 t
ORIGIN      
        1 cgtcgccccg accgggctgc ccgtggagtc ggcagtggcc gacgtccggg acgcgctggc
       61 caccgtgggc accgcggtgc tccaggccga gccgggagcg ggcaagacca ccgtggtgcc
      121 gttgcgcctg ctcgacgagg cgtggcgcgg tgacgggcgc atcgtggtcc tcgagccgcg
      181 gcgcctggcc acccgggccg cggcgcgacg gatggccgac ctcctgggcg agcgcgtcgg
      241 agagaccgtc ggctaccgca cccgggacga acgtcacgtc ggaccggcga cccggatcga
      301 ggtcgtgacc gaggggatcc tcacccgtcg gctccaggcc gacccgagcc tgcccggcac
      361 cgcgctggtc gtcttcgacg aggtccacga gcggcacctg cagaccgacc tcgccctggc
      421 gctcgccctc gacgcccgcg agggcctgcg cccggatctc cggttgctgg ccatgtcggc
      481 cacgctcgag gccacccggc tggccgtcct gttgggcggg tccgccggac cggcgccggt
      541 cgtgaccagc cccggccgca cccaccccgt cgacatccgt tggcggcccc ccgggccccg
      601 ggtgcgtccc ttcgatgccg tcgccgccgt cgtgcacgag gcggtccgcg acgaaggcgg
      661 tgacgtgctc gtgttcctcc ccggggccgc cgccatccgg cgcgtcggtg ggttgctggc
      721 cgacctgccc cgcgacgtcg acgtccggcc gctgcacggc agcctccgcc ccgaggagca
      781 ggacctcgcc ctcgccccgt cggcgtccgg gcggcgacgg gtcgtgctgg ccaccgacat
      841 cgccgagtcg agcctgaccg tcgacggtgt ccgggtcgtg gtcgacgccg gcgaggtccg
      901 ccggccgcgc catgacaccc gcagcgggct gagccgcctg cacaccaccg ccgcctcgcg
      961 ggcctcggcg gtccagcggg cgggccgggc cggacggctc gggccgggcg tcgcctaccg
     1021 gctgtggtcg gcgggcgagc acgccgcccg ccggccgttc gcgccgcccg agatcgccgc
     1081 cgccgacctg gccgggttgg ccctcgagct ggccgtctgg ggggcgacgg ccgacgctct
     1141 ggccttcctc gacccgcccc cgcccaccgc cctggccgac gccaccgagc tgctcggtcg
     1201 cctcggggcg atcgacgacg ccgggcggcc gacgccgacc gggcggacga tggtcgacct
     1261 cccgttgcac ccccgcctgg cccgcatggt gaccgacgag cgctccgccc tggccgtcgt
     1321 cctggccacc ctgctcgagg aacgggaccc gctgcgaggg cgacccgagg agctggacgc
     1381 cgacgtcacc gagcgcattc gcctggtgac ggacccccgc cggggccatc cccggctcga
     1441 cgacggagcc cggcgcaccg cgcgccgacg ggcccaggaa ctggcccggc gggcgggcat
     1501 cggcttcggg gaggtcgacc cgagccgggc agggcccgtc ctggccctcg cctaccccga
     1561 ccgcatcgcc caggcccgcg ggcgagggcg gttccagctg cggtcgggac gggccgtggc
     1621 cctggccccc cacgacccgc tggccgccga agcgttcatc gtcgtggccg atctggcccc
     1681 gcccgcccct cgggaggtcg acgaccgggt ccgtctcgcc gcggccctcg acgaggccga
     1741 ggtcgaggcg gtggccggga ccgaggttcg ggagacgacc gagctggcct ggaacgcccg
     1801 ggacgagctc gagcagcgcg ccgagcggcg cctcgacgcg ttggtgctgg accgggccgt
     1861 cgggcccgcc cccgccgggg cgccgacgac ggccgcgctc ctcgaccggg tgcgggacga
     1921 cggcctcggc ctgctgggct ggaccacggc ggcccgcacg cttcaggagc gggccggctt
     1981 cgcccggcgt ttcctcggcg acccgtggcc ggacctcaac gacgaggccc tcgtcgccgg
     2041 catggccgag tggctggctc ccctcctcgg gcgagccacc tcgcgcgccg acctccgtcg
     2101 ggtcgacgtg ctggccgtgc tccgagggcg ccttggtcac cgcctgcacg agctcgaccg
     2161 cgtcgtgccc gcccgggtga cgatcgccag cggccggacg gtggcgggcg actatgacca
     2221 cgaccccccg tccatcaggg tccgggccca ggagctctat ggcaccgcga tccatcccac
     2281 cgtggccgat ggtcgggtgc ccctcaccgt cgaggtcctc tcccccgccg gtcgcccggt
     2341 ccaggtgacc
//
LOCUS       DASTFV010000170         4482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_598868, whole genome shotgun sequence.
ACCESSION   DASTFV010000170 DASTFV010000000
VERSION     DASTFV010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598868"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VFP02_03215"
     CDS             <1..501
                     /locus_tag="VFP02_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET9542063.1"
                     /translation="LAPAAHGANRTGRMFTGDRSGDFLYAALHRTGFANQPTSDHRDD
                     GLTLTGAWITAPVRCAPPANKPTPEERDTCRPWLERELALLPSLEVFVVLGQFAYQAM
                     TNHLQVKPRPKFAHGTEVPAPRGQTILCSYHVSQQNTFTGKLTEKMFDEVLQRARALA
                     AAGGEM"
     gene            695..1111
                     /locus_tag="VFP02_03220"
     CDS             695..1111
                     /locus_tag="VFP02_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542064.1"
                     /translation="MGRPRALRLGVALVVTVFLGVACSGDDTADYDAAYQEDFLRQCR
                     DAFGGAAAPQVCGCWYDALSRTVAFKDVPAIDDMLADDFDDAPTRLPGGEMDVPLELL
                     AACVRSLGATPTLGDPVPLPTTPRPPPPPTTTTVVT"
     gene            1108..1479
                     /locus_tag="VFP02_03225"
     CDS             1108..1479
                     /locus_tag="VFP02_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542065.1"
                     /translation="MSKLVVGAAAVVVLAAAVVGGILFVRDDGPPTDYDARIEEDFMA
                     TCTADAENLDFSRSEDFCRCAYDGIRADIPFDRFLAIDAALQADPTAVPGPVDRIRTA
                     CYFETEADPTLSTVPTTTAPA"
     gene            1485..2693
                     /locus_tag="VFP02_03230"
     CDS             1485..2693
                     /locus_tag="VFP02_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HET9542066.1"
                     /translation="MTTTEPAPARTRWTYQWKELYEEVIDFGLCTGCAGCVIACPHDV
                     IGYDHEQGGYKPFHLEDELGPTDCGHGQKGCTSCTRACPRFRTWEEDANEHLFAQDRE
                     PDDMAGIYKDILLTRASDDMVHEMGQDGGLVSALLIWAMDKGYIDGALTSFLEGDGST
                     WKAIPGVAANKEEILASAGSRYTYSANPLALKEAQERGLSKLALVGMGCQTSSPPVMW
                     HRKVGKVGKPFILNIGLMCSKTFDDAIFEELFEAKYGIKKADIKKVNIKGVFQIWCHD
                     GSYHEVNLKECHAWTREGCKSCPDFAAEHADISTGGIGKYNDWTLTVVRTDLGREVIS
                     RMLADGTIVSRPGDDDPDAIKLMRRLSIVSRRRWPDTAWAPVKLGTPPPKKKAEAPPA
                     AEPPPEVANT"
     gene            2716..3954
                     /locus_tag="VFP02_03235"
     CDS             2716..3954
                     /locus_tag="VFP02_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9542067.1"
                     /translation="MDLTYPPEAEEFRLEVRSWLEENLPDGWFDEGFEMTPEERKAFN
                     RGWTAKLHEGNWLCASWPTEYGGRGLPTMHAVVLAEEFHRSGAPMRADFFGDTLVGPT
                     ILQWGTEEQKQEFIPKILSGEISWCQGFSEPDAGSDLAGLKTKAELDGDEWVVNGQKI
                     WTTQAGFADYIFVLARTDPDAPKHKGISYLLCPMKQPGIEVRPIKQIDASAEFYEVFF
                     TDARCPKENVVGGVNGGWKVAMTTLGFERGTSATTGYRRFEKELEGITQMAKANGKAD
                     DPRVRQGLADAWSKVQIMKVNGLRSLSSVLTGSKDLGVAALGATNKMFWSEYHRDVME
                     LAIDIAGMGGQVLTGNVDAEESVPGYGARRTNEGYPASVLQSAFFFSRSETIWGGTAQ
                     IQRNIVGERVLGLPKEPRPD"
     gene            3956..4369
                     /locus_tag="VFP02_03240"
     CDS             3956..4369
                     /locus_tag="VFP02_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HET9542068.1"
                     /translation="METDEARALVASARVARLATVNPGLGVDVVPITFALLEGDRLVS
                     IVDQKPKSTTRLRRLTNIRAHPEVTVLVDHYEDDWERLWWVRMRGTATVIAEGDDFTE
                     AVDALTERYPQYVAAPPPGPVVSVAVDAWSGWRAD"
BASE COUNT          733 a         1632 c         1480 g          637 t
ORIGIN      
        1 ctggcgcctg cggcccacgg ggccaaccgg accgggcgca tgttcaccgg cgaccgatcg
       61 ggcgacttcc tctacgccgc cctgcaccgc accggcttcg ccaaccaacc cacctcggac
      121 caccgcgacg acggcctgac cctcaccggc gcctggatca ccgcccccgt ccgctgcgcc
      181 cccccggcga acaagcccac ccccgaagag cgcgacacct gccgcccgtg gctcgagcgg
      241 gagcttgcgc tcctccccag cctcgaggtc ttcgtcgttc tcggccaatt cgcctaccag
      301 gccatgacga atcaccttca ggtgaagcct cggcccaagt tcgcccacgg gacagaggta
      361 ccggccccac gaggccagac gatcctgtgc agctaccacg tgagccagca gaacaccttc
      421 accggaaagc tcacggagaa gatgttcgac gaggtgctgc agcgggcgag ggcgctggct
      481 gcggcggggg gcgagatgta gcgcccgcct ggcggggacc ggagcgccgc cgaattggct
      541 ccggtccccg caccggccgg ttgaggaggg gactcgtcgt tgagacccca ccagagtggc
      601 cgtgcaggcg ctacctgaac ttgatcggtc acctccgcgg gtctccatat gggccatcag
      661 gcctgttttt ggccgcctgg gtacggtgcg ggccatgggt cggcctcgtg cgctacgtct
      721 cggcgtggcg ctggtcgtga ccgtgttcct cggggtcgcc tgcagtggtg acgacacggc
      781 cgactacgac gccgcctacc aggaggactt cctccgccag tgccgcgacg ccttcggggg
      841 tgccgccgcc ccgcaggtgt gcggctgctg gtacgacgcc ctgtcccgaa cggtggcgtt
      901 caaggacgtg cccgccatcg acgacatgct cgccgacgac ttcgacgacg cccccacccg
      961 tctgcccggc ggtgagatgg atgtgcccct cgagctcctg gccgcgtgtg tccgctccct
     1021 gggggccacg ccgacgctgg gcgatcccgt gcccctgccg acgacgcctc gacccccgcc
     1081 cccgccgacc acgaccacgg tcgtgacgtg agcaagctgg tggtcggggc cgccgccgtg
     1141 gtggttctgg ccgccgcggt cgtgggtggc atcctcttcg tgcgggacga cggcccgccc
     1201 accgactacg acgcccgcat cgaggaggac ttcatggcca cgtgcacggc cgatgccgag
     1261 aacctcgact tctcgcgatc cgaggacttc tgccggtgtg cctacgacgg catccgcgcc
     1321 gacatcccct tcgaccggtt cctggccatc gacgccgctc tccaggccga tcccaccgcg
     1381 gtgcccggcc cggtcgaccg gatccgcacc gcctgctact tcgagaccga ggctgacccg
     1441 acgctgagca ccgtcccgac caccaccgcg cccgcctgag gaccatgacc accactgagc
     1501 ccgcccccgc ccgcacccgc tggacctacc agtggaagga gctctacgag gaggtcatcg
     1561 acttcggcct ctgcacgggc tgtgcgggct gtgtgatcgc ctgcccccac gacgtcatcg
     1621 ggtacgacca cgagcagggt ggctacaagc cgttccacct cgaggacgag ctgggcccca
     1681 ccgactgcgg ccacggtcag aagggctgca ccagctgcac gcgggcctgc ccccgcttcc
     1741 gcacctggga ggaggacgcc aacgagcacc tcttcgccca ggaccgagag cccgacgaca
     1801 tggccgggat ctacaaggac atcctcctga cccgggcctc cgacgacatg gtccacgaga
     1861 tgggccagga cggcgggctg gtctcggccc tgctgatctg ggcgatggac aagggctaca
     1921 tcgacggcgc cctcacctcg ttcctcgagg gggacgggtc gacgtggaag gccatccccg
     1981 gcgtggccgc caacaaggag gagatcctgg cctccgccgg cagccgctac acgtactcgg
     2041 ccaaccccct ggcgctgaag gaggcccagg agcggggcct gtccaagctg gctctcgtcg
     2101 gcatggggtg ccagacctcg tcgccgccgg tgatgtggca ccgcaaggtc ggcaaggtcg
     2161 gcaagccgtt catcctgaac atcggcctga tgtgctcgaa gacgttcgac gacgccatct
     2221 tcgaagagct cttcgaggcg aagtacggga tcaagaaggc ggacatcaag aaggtcaaca
     2281 tcaagggggt gttccagatc tggtgccacg acggcagcta ccacgaggtc aacctcaagg
     2341 agtgccacgc ctggacccgg gaaggctgca agagctgccc ggacttcgcc gccgagcacg
     2401 ccgacatctc gacggggggc atcggcaagt acaacgactg gacgctcacg gttgtccgca
     2461 ccgacctggg ccgggaggtc atctcccgga tgctggccga cggcaccatc gtcagccggc
     2521 ccggcgacga cgaccccgat gccatcaagc tgatgcgccg cctctccatc gtcagccgcc
     2581 ggcgctggcc cgacaccgcc tgggctcccg tcaagctcgg caccccaccc ccgaagaaga
     2641 aggcggaggc gccgcccgcc gcggagccac cgcctgaggt ggcaaatacc tgaccgactg
     2701 gtcaagctct cctccatgga tctgacctat ccccccgagg ccgaggagtt ccggctcgag
     2761 gtccgctcgt ggctggagga gaacctgccc gacggctggt tcgacgaggg gttcgagatg
     2821 accccggagg agcgcaaagc gttcaaccgg gggtggacgg ccaagctcca cgagggcaac
     2881 tggctgtgcg cctcgtggcc gaccgagtac ggcggccgag gcctgcccac gatgcacgcc
     2941 gtcgtcctgg ccgaggagtt ccaccgctcc ggcgccccga tgcgggccga cttcttcggc
     3001 gacaccctcg tcggtcccac gatcctgcag tggggcaccg aggaacagaa gcaggagttc
     3061 atccccaaga tcctcagcgg ggagatctcc tggtgccagg gcttctcgga gcccgatgcc
     3121 ggctccgacc tggccgggct caagaccaag gccgagctgg acggcgacga gtgggtcgtc
     3181 aacggccaga agatctggac cacccaggcc ggcttcgccg actacatctt cgtgctggcc
     3241 cgcaccgacc cggacgcacc gaagcacaag ggcatctcct acctgctctg cccgatgaag
     3301 cagccgggca tcgaggtccg ccccatcaag cagatcgacg cctcggcgga gttctacgag
     3361 gtcttcttca ccgacgcccg ctgcccgaag gagaacgtgg tcggtggggt caacgggggc
     3421 tggaaggtgg ccatgaccac cctcggcttc gagcggggga cctcggccac cacgggctac
     3481 cgccgcttcg agaaggagct ggagggcatc acccagatgg ccaaggccaa cggcaaggcc
     3541 gacgacccgc gagtgcgcca aggcctggcc gacgcctggt ccaaggtcca gatcatgaag
     3601 gtcaacggtc tgcgctcgct gtcgtcggtc ctcaccggga gcaaggacct cggcgtggcc
     3661 gccctgggtg ccaccaacaa gatgttctgg tcggagtacc accgcgacgt catggagctg
     3721 gcgatcgaca tcgccggcat gggcggccag gtgctcacgg gcaacgtgga cgcggaggag
     3781 tcggtgcccg gttacggcgc ccggcgcacc aacgaggggt atccggccag cgtgctgcag
     3841 tcggcgttct tcttcagccg atccgagacc atctggggcg ggacggccca gatccagcgc
     3901 aacatcgtgg gggagcgggt gctgggcctg cccaaggaac cccggcccga ctgacgtgga
     3961 gaccgacgag gcccgggcgc tggtcgcctc ggcccgggtg gcccgcctgg ccaccgtcaa
     4021 ccccggcctc ggcgtcgacg tcgtccccat caccttcgcc ctgctcgagg gtgaccggct
     4081 ggtcagcatc gtcgaccaga agcccaagtc gaccacccgc ctgcgccgct tgaccaacat
     4141 ccgggcccac cctgaggtga ccgtgctggt cgaccactac gaggacgact gggagcgcct
     4201 gtggtgggtc cgaatgcggg ggacggccac ggtgatcgcc gagggcgacg acttcactga
     4261 ggccgtggat gccctgaccg agcgctaccc gcagtacgtc gccgccccgc cccctggccc
     4321 cgtcgtctcc gtcgccgtcg acgcctggtc gggctggcgg gccgactgag cgccgtccgg
     4381 gcccgacatt gcggttcggt tacagagcac tacccttccg gccgcatggc gaagcgcagg
     4441 cgcggacggc gcgccgactc gtcctccggc gaaccggccc cc
//
LOCUS       DASTFV010000171         2524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_602886, whole genome shotgun sequence.
ACCESSION   DASTFV010000171 DASTFV010000000
VERSION     DASTFV010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602886"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="VFP02_03245"
     CDS             <1..1504
                     /locus_tag="VFP02_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HET9542069.1"
                     /translation="PPLSGDEMPHLLDRYAEAKTESRVIDFDDLLRLALRIMERDAPA
                     AAAQRWRFRHLFVDEFQDVNPLQYRLLRAWLGDADDLFVVGDPHQAIYGWNGADARYL
                     DRFEEVFPAERYPGVAVLHLRGNYRSTPQILATAAAVLETSRLVAHRPDGPMPTVVAH
                     ADEAAEARAITRDVRERHGPGRPWSTQAVLVRTNGQGAVITEALSRAGIPHRLRGADQ
                     LLGRPGVVDVLRRLRREPYAAVLADLRSPPVDAEPEATPRAAEDADQATEDRATLVIL
                     AREYEALDPEPSGPAFASWLVAAVRSGEGADKDVVEVSTLHAAKGLEWPVVHLAGLED
                     GLVPIGYAKTPAARAEEQRLLYVGVTRAKDEVRLHWSRTRVQGGEARDRSPSPWLPPV
                     EAAITDLLVASRPVDGRHHLQTPRAELHARHPAPAEEVDEVLARLAAWRAQTARVARV
                     APQAVVDDRTLHAIAEARPASVEALTGLPGLGPIKAARFGDGILAALDPS"
     gene            complement(1608..2324)
                     /locus_tag="VFP02_03250"
     CDS             complement(1608..2324)
                     /locus_tag="VFP02_03250"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET9542070.1"
                     /translation="MSGEPREMSDEDRGMVSERWREVEADDLTPRQREQRRLADRMRT
                     VIDRLVATSAPEEALRYAADSLERLAAEFEQYPQGNTYEGFAEAANAGGDQQAFFDHS
                     PLIGLANPLSPPVVIRVEDDHIDGRVTFGAAYEGPPGCVHGGYIAAAFDEVLGSAQSL
                     GGRPGMTGTLTIRYRSPTPLHTPLRFEAGVTGVDGRKIFTTGRCFADDVLTAEAEGIF
                     ISIDRERVEELLALRDGTPD"
     gene            complement(2321..>2524)
                     /locus_tag="VFP02_03255"
     CDS             complement(2321..>2524)
                     /locus_tag="VFP02_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9542071.1"
                     /translation="RQRYEGGGLFSWWDYRAGNFHKGKGMRIDHVLATPPLAERAGAV
                     LIDRNARKGEKPSDHAPVVVDFT"
BASE COUNT          330 a          975 c          884 g          335 t
ORIGIN      
        1 cccaccgctg tccggcgacg agatgcccca cctcctcgac cgctacgccg aggccaagac
       61 cgagtcccgg gtcatcgact tcgacgacct gctccggctg gcgttgcgga tcatggagcg
      121 ggacgccccc gccgcagccg cccagcgctg gcgcttccgc cacctcttcg tcgacgagtt
      181 ccaggacgtc aacccactgc agtaccgact cctgagggcc tggctgggcg acgccgacga
      241 cctgttcgtc gtcggcgatc cccaccaggc catctacggc tggaacgggg ctgacgcccg
      301 ctacctggac cgcttcgagg aggtgttccc cgccgagcgc taccccggtg tcgccgtcct
      361 gcacctgcgg gggaactacc gctcgacccc gcagatcctg gccacggccg cggccgtcct
      421 ggagaccagc cgtctcgtcg cccaccgacc ggacgggccg atgccgacgg tcgtggccca
      481 cgccgacgag gccgccgaag cccgggccat cacccgggac gtccgcgagc gccacggccc
      541 gggacggccg tggtcgaccc aggccgtgct cgtgcgcacc aacggccaag gggccgtgat
      601 caccgaggcc ctcagtcggg cgggcatccc ccaccgcctc cgtggtgccg accagctact
      661 cggccggccc ggcgtggtcg atgtcctccg ccgcctgcgc cgcgagcctt acgccgccgt
      721 gctggccgat ctccggtcgc ctccggtcga cgcagagccc gaggccacac cccgcgccgc
      781 cgaagacgcc gaccaggcca ccgaggaccg ggccaccctc gtgatcctgg cccgtgagta
      841 cgaggccctc gaccccgagc cgtcgggccc ggccttcgcc agctggctgg tcgccgccgt
      901 gcgctcgggc gagggggcgg acaaggacgt cgtcgaggtc agcaccctgc acgcggcgaa
      961 gggcctcgaa tggcccgtcg tgcacctggc ggggctggag gacggcctgg tcccgatcgg
     1021 ctacgcgaag acgccggcgg cccgggccga ggagcagcgc ctgctctacg tgggcgtcac
     1081 ccgggccaag gacgaagttc gcctgcactg gtcccgcacc cgggtccagg ggggcgaagc
     1141 ccgtgaccgg agcccgtccc cgtggctccc gccggtcgag gccgcgatca ccgacctcct
     1201 ggtcgcgtcc cgccccgtcg acggccgcca ccacctccag acgccgcggg ccgagctgca
     1261 cgcccgtcac cccgccccgg cggaggaggt cgacgaggtc ctcgcccgac tggccgcctg
     1321 gcgggcccag accgcacggg tggcccgcgt ggccccgcag gccgtcgtcg acgaccgcac
     1381 cctccacgcc atcgccgagg cccgcccggc ctccgtcgag gcgctgaccg gtctccccgg
     1441 gctgggcccg atcaaggccg cccgcttcgg cgacgggatc cttgccgccc tcgacccgtc
     1501 ctgagcgaag atgggccagc gggtcggccg gccgactgca acgtcgtgat cgccacgggg
     1561 ttcgtgctgg gcgacgccgg gaggtcctga gcaccggcag gcaacgatca gtcgggggta
     1621 ccgtcccgca gggcgagcag ttcttcgacc cgctcccggt cgatgctgat gaagatgcct
     1681 tcggcctcgg cggtgaggac gtcgtcggcg aagcacctgc cggtggtgaa gatcttgcgc
     1741 ccgtccacgc cggtcacccc cgcctcgaag cgcagtggcg tgtgcagggg cgtgggcgag
     1801 cggtagcgga tggtcagcgt gccggtcatg ccgggacggc cgccgaggga ctgggcggaa
     1861 ccgaggacct cgtcgaaggc ggcggcgatg tagccaccgt ggacgcaacc cggcgggccc
     1921 tcgtacgcgg cgccgaaggt cacccggccg tcgatgtggt cgtcctcgac ccggatgacc
     1981 accggcggcg acagcggatt ggccaggccg atcagcggac tgtggtcgaa gaacgcctgt
     2041 tggtcaccgc cggcgttggc cgcctcggcg aagccctcgt aggtgttgcc ctgcgggtac
     2101 tgctcgaact cggcggccag gcgctcgagg gagtcggcgg cgtaccgcag cgcttcctcc
     2161 ggggcggagg tggccacgag gcgatcgatc accgtgcgca tccgatccgc caggcgccgc
     2221 tgctcgcgct ggcgcggcgt gaggtcgtcg gcctcgacct cccgccaccg ttcgctcacc
     2281 atcccgcggt cctcgtccga catctcccgc ggctcgccgc tcatgtgaag tccaccacca
     2341 ccggcgcgtg gtccgacggc ttctcgccct tgcgggcgtt ccggtcgatg aggacggccc
     2401 cggcccgctc ggccagcggc ggcgtggcca ggacgtggtc gatgcgcatg cccttgccct
     2461 tgtggaagtt gccggcacgg tagtcccacc acgagaacag gccgccgcct tcgtagcgct
     2521 gccg
//
LOCUS       DASTFV010000172         2636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_609618, whole genome shotgun sequence.
ACCESSION   DASTFV010000172 DASTFV010000000
VERSION     DASTFV010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609618"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VFP02_03260"
     CDS             complement(<1..565)
                     /locus_tag="VFP02_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542072.1"
                     /translation="MTADAPTTEFDPDAADDAEPGGFLPVLTSVIQRRWTSSVFGIVA
                     PGAVRRRPSDVARILTAIVLIAAISASVTSITAIEAKVIAVVAALPDGFTGVGEVLYR
                     LGPIVAGGLAVSALVARRTRLLLTLLVAFAVGLGFGALLSELVDITQALKDAGTDLAG
                     LNPDFPVVPLAASIAVLLAARPYLTRPT"
     gene            complement(633..2024)
                     /locus_tag="VFP02_03265"
     CDS             complement(633..2024)
                     /locus_tag="VFP02_03265"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HET9542073.1"
                     /translation="MFHHSKNDANDALAPTYGGRFLSEPIPKYRIPEGEMPARVAYQI
                     IHDELALDGNPALNLATFVTTWMEPEAEALMTESLNVNSIDQDEYPATTEIQNRCVSI
                     MADLFHADDAEHAMGTATVGSSEAIHLAGLAMKWRWRSRQEAAGKPTDRPNLVMSETV
                     QVCWEKFARYFDVEARMVPITPERTVIDPELAMALVDENTIGVVGILGSTYTGEFEPI
                     GELAAALDDVQERTGLDVPIHVDGASGAFVAPFTQPKLAWDFRLPRVKSINASGHKYG
                     LVYPGVGWALWRDTAHLPEDLIFHTNYLGGDQPTFDLNFSRGASQIIAQYYNFLRMGR
                     AGYTDVMAGLSETATYLAGLVDNSPILELMSKPDALPLVCAKVADDAGFTAYDLSAKM
                     KERGWIVPAYTLAPDADHVSVLRVVVREGLSSEMADILVADFHRAGEALGGTLPELPT
                     RGTHQPHKRGKIC"
     gene            complement(2079..>2636)
                     /locus_tag="VFP02_03270"
     CDS             complement(2079..>2636)
                     /locus_tag="VFP02_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542074.1"
                     /translation="VGSFCFALGSVPGFASLVAANTVVLVFFVGSLFFTSAATLQYLQ
                     TTGGKLWSWQPTDLAWAASLIQLVGTLWFNIDTAAARLTGLDAQQENLRVWTPDYLGS
                     VCFLVASLLAVRTLSRPPWHAGRPWKVAGLNLLGSVFFMAAAVAAFVVPDTDDLLDAS
                     LANSGTFLGAICFLVAARIEITPDE"
BASE COUNT          409 a          835 c          994 g          398 t
ORIGIN      
        1 gggtcgggcg ggtgaggtag ggccgggcgg cgaggaggac ggcaatggac gcggccaggg
       61 gaacgaccgg gaagtccggg ttgagcccgg cgaggtccgt tccggcgtcc ttcagggcct
      121 gggtgatgtc gaccagctcc gagaggagcg ccccgaagcc cagcccgacg gcgaacgcca
      181 cgagcagggt gaggagcagg cgggtacggc gggcgaccag ggcgctgacc gccaaccccc
      241 ccgccacgat cggaccgagc cggtagagga cctcgcccac cccggtaaat ccgtcgggca
      301 gggcggccac gacggcgatc accttcgcct cgatggcggt gatcgacgtg acgctggcgg
      361 agatcgcggc gatcagcacg atggcggtga gtatccgggc gacatcactc ggccgtcggc
      421 gcaccgcgcc gggggccacg atcccgaaga ccgacgacgt ccagcgtcgt tggatcaccg
      481 aggtcaggac cggcaggaaa ccccccggct cggcgtcgtc agccgcgtcc gggtcgaact
      541 cggtggtcgg ggcgtcggcg gtcatcggcc ccgtcccgtc atcgggccga tcttaggttg
      601 ggcgcagtgg cggacccggg gggcgccgcg actcagcaga tcttgccccg cttgtgcggc
      661 tggtgggtgc ctcgcgtcgg cagctcgggc agcgtgcctc cgagcgcttc gccggcgcga
      721 tggaagtcgg cgaccaggat gtcggccatc tccgagctga gcccctcgcg caccacgacc
      781 cggaggaccg agacgtggtc ggcgtcgggc gccagcgtgt acgcgggcac gatccagccc
      841 cgttccttca tcttggcgct gaggtcatag gccgtgaacc cggcgtcatc ggccaccttg
      901 gcgcacacca gggggagggc gtcgggtttg ctcatcagct cgaggatggg gctgttgtcc
      961 acgagaccgg cgaggtaggt cgccgtctcc gacaaaccgg ccatcacgtc cgtgtacccc
     1021 gcccgaccca tgcgcaggaa gttgtagtac tgggcgatga tctggctggc cccccgggag
     1081 aagttcaggt cgaaggtggg ctggtcgcct ccgaggtagt tggtgtggaa gatgaggtcc
     1141 tcgggcaggt gggccgtgtc ccgccacagc gcccaaccga cgcccgggta cacgaggccg
     1201 tacttgtgtc cggaggcgtt gatcgacttc acccggggga gacggaagtc ccaggccagc
     1261 ttcggttggg tgaagggggc gacgaaggcc ccgctggcgc cgtcgacgtg gatcgggaca
     1321 tccagcccgg tccgctcctg gacgtcgtcg agggcggcgg cgagctcgcc gatcggctcg
     1381 aactcaccgg tgtaggtgga gccgaggatg ccgaccaccc cgatggtgtt ctcgtcgacg
     1441 agggccatgg ccagctcggg gtcgatgacg gtgcgttcgg gggtgatcgg caccatccgg
     1501 gcctcgacgt cgaagtagcg ggcgaacttc tcccagcaga cctggacggt ctcgctcatg
     1561 accaggttgg gccggtcggt cggcttcccg gcggcctcct ggcgggaccg ccagcgccac
     1621 ttcatggcca ggccggccag gtggatggcc tcggaggagc ccaccgtggc ggtgcccatg
     1681 gcgtgctcgg cgtcgtcggc gtggaacagg tcggccatga tgctgacgca ccggttctgg
     1741 atctcagtgg tcgccggata ctcgtcctgg tcgatgctgt tgacgttcag gctctcggtc
     1801 atcagggcct cggcctcggg ctccatccag gtcgtgacga aggtggccag gttcagcgcc
     1861 gggttgccgt ccagggccag ctcgtcgtgg atgatctggt aggcgacgcg ggccggcatc
     1921 tcgccctcgg ggatccggta cttcgggatc ggctcggaga ggaaacggcc gccgtacgtc
     1981 ggcgcgagag cgtcgttggc gtcgttcttg ctgtgatgga acatgagagt cctttggtcg
     2041 tccaggagga tgattccacg accggggccg cccgttcatc attcgtcggg ggtgatctcg
     2101 atccgggcgg ccaccaggaa gcagatcgcc cccaggaagg tcccgctgtt ggccaggctg
     2161 gcgtcgagga ggtcgtccgt gtcgggcacc acgaaggcgg cgacggcggc ggccatgaag
     2221 aacaccgagc cgagcaggtt caggccggcc accttccagg ggcggccggc gtgccatggc
     2281 gggcggctga gcgtcctgac ggctaggagg ctggccacga gaaagcacac cgagccgagg
     2341 tagtccggcg tccacacccg caggttctcc tgttgggcgt ccaagccggt caggcgggca
     2401 gccgcggtgt cgatgttgaa ccagagggtc ccgacgagct ggatgagcga ggccgcccag
     2461 gccaggtcgg tcggctgcca cgaccacagc ttcccgccag tggtctgcag gtactggagc
     2521 gtggctgcgc tggtgaagaa cagcgagccg acgaagaaga cgaggacgac cgtgttcgcg
     2581 gccacgaggc tggcgaaccc gggcacgctg ccgagggcga agcagaacga gcccac
//
LOCUS       DASTFV010000173         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_616656, whole genome shotgun sequence.
ACCESSION   DASTFV010000173 DASTFV010000000
VERSION     DASTFV010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616656"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFP02_03275"
     CDS             <1..208
                     /locus_tag="VFP02_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542075.1"
                     /translation="LDAALSADDDTTTHEQAAAFLDTARGTPFESRPGVSLGDDVQAE
                     TDELVATGIGWGGELLHLVCFAQA"
     gene            573..1832
                     /locus_tag="VFP02_03280"
     CDS             573..1832
                     /locus_tag="VFP02_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9542076.1"
                     /translation="MRIAIVGSGVSGLVCAHLLHSRHEVTLYEVDGRPGGHANTVEVD
                     VDGERHAVDTGFIVYNERTYPGFVALLAHLGVATKPSDMSFSVADERTGLEWRSTSLS
                     TVFAQRRNLADPAFLRMLADVARFNRAARRVAADGVEGDLTLGEFLASGRWSRQLLDG
                     YLVPLGSAIWSADPRSFADIPLITFARFFDNHGLLQLGDRPRWRTIEGGSAHYVDAIL
                     RPLRGHVRCNDGIEKVVRRRQTGTVELRTAVGALREFDHVVIATHSDQALRLLADPTP
                     AEREILGAIRYQPNRATLHTDRGLLPRNRRAWASWNYHRMADDRSQVAVTYSMNRLQG
                     IPSPTPVLVTLNRHDDIDPARVLDTFEYDHPVLDAAAVRAQGRHGELSGHEGTSFAGA
                     YWGYGFHEDGVQSALRVCRALGVEPPW"
     gene            1829..2560
                     /locus_tag="VFP02_03285"
     CDS             1829..2560
                     /locus_tag="VFP02_03285"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HET9542077.1"
                     /translation="MNSALYEGTLRHRRRGPRPHRFDYALTLLYLDLAELDEVFARHP
                     LWSVERPNAVSFRRADFLGDTEIPLDTAVRDLVEARLGRRPSGAIRLLGHVRTWGWLF
                     NPLTIYVCTDAGGDRIEALVLEVTNTPWHERHAYVLDGGAGEHRFAKALHVSPFFGMD
                     QEYRLRMGAPGEHLVVELASLEGGAVVFEASLALRRRAITRSSLGRLLWRHPLLTLRV
                     STAIYTQALRLWAKRTPYHPHPARS"
BASE COUNT          361 a          974 c          855 g          377 t
ORIGIN      
        1 gctcgatgcg gcgctctccg ccgacgacga caccacgacg cacgagcagg cggcggcctt
       61 tctcgacacg gcccgaggta ccccgttcga gtcccgtccc ggggtgtcac tcggcgatga
      121 cgtgcaagcc gagaccgacg agctggtggc gaccggcatc ggttggggcg gcgaactcct
      181 ccacctcgtc tgcttcgccc aggcctgagc tggcacaaac cgctccttgg agatcgccgt
      241 ggccagcagg catcagctgg cacgtcgcga tgcagggaga cgtaggtatc aaccacgcgc
      301 cggtcagagg atggatccga atgcgtctcg atgaggtcat ccgccgggcc gtcatcctcg
      361 gtctctgcgt gatagacgcg gtcgtcggcg acgatcgtga ccgcccgatg cgcccggcct
      421 ggcgacggcg gggtgctgcg cctgctcagg cgaaccaggc cgcgacggac aactcccagg
      481 gtgctccgat cacgtcaagg ggtgggccgg gaccggtcat gcgacgacct ccgaggttgt
      541 ctccttcttt agtgctaaat agcatccacc tgatgcggat tgcgatcgtg ggcagtgggg
      601 tgtcgggcct cgtctgcgcc cacctgctgc actcccgcca cgaggtcacc ctgtacgagg
      661 tcgacggacg gccgggcggg cacgccaaca cggtcgaggt cgacgtcgac ggtgagcgcc
      721 acgccgtcga caccggcttc atcgtctaca acgagcgcac ctaccctggt ttcgtcgccc
      781 tgctggccca cctcggcgtg gccaccaagc cgagcgacat gagcttcagc gtggccgacg
      841 agcgcaccgg actggagtgg cggagcacct ctctctcgac cgtgttcgcc cagcgccgga
      901 acctggccga cccggccttc ctgcgcatgc tcgccgacgt ggcccggttc aaccgagcgg
      961 cccggcgagt cgccgccgac ggcgtggagg gcgacctcac actcggggag ttcctggcgt
     1021 cggggcggtg gtcgcgccag ctcctcgacg gctacctcgt ccccctgggc tcggccatct
     1081 ggtcggctga cccgaggtcg ttcgccgaca tccccctcat caccttcgcc cgcttcttcg
     1141 acaaccacgg cctgctgcag ctgggcgacc ggccccgctg gcgcacgatc gagggcggct
     1201 ccgcccacta cgtggacgcc atcctccgcc cgctgcgcgg gcacgtgcgg tgcaacgacg
     1261 ggatcgagaa ggtcgtccgg cgcaggcaga cggggacggt cgagctccgg acggcggtcg
     1321 gggccctgcg ggagttcgac cacgtggtca tcgccaccca cagcgaccag gccctgcgcc
     1381 tcctggccga cccgacgccg gcggaacgtg agatcctcgg ggccatccgc taccagccca
     1441 accgggccac gctccacacc gaccgggggc tcctgccccg gaaccggcgg gcgtgggcga
     1501 gctggaacta ccaccgcatg gccgacgacc ggagccaggt ggccgtcacc tactccatga
     1561 accggctcca ggggatcccg tcgccgacgc cggtcctggt cacgctcaac cgccacgacg
     1621 acatcgatcc ggcccgggtc ctggacacct tcgagtacga ccaccccgtg ctcgatgccg
     1681 cggcggtgcg ggcacagggc cgccacggcg agctcagcgg ccacgagggc acgtcgttcg
     1741 ccggcgccta ctggggctac gggttccacg aggacggggt gcagagcgcg ctgcgggtgt
     1801 gccgggccct gggggtcgag ccgccgtggt gaacagcgcc ctctacgagg gcacgctacg
     1861 ccaccggcgc cggggccccc gcccccaccg gttcgactac gccctgaccc tgctctacct
     1921 cgacctcgcc gagctcgacg aggtcttcgc ccgacacccg ctctggtcgg tcgagcgccc
     1981 caatgcggtc tcgttccgac gggccgactt cctgggcgac accgagatcc ccctcgacac
     2041 tgcggtgcgc gacctcgtgg aggcccggct cggccgacgg ccttccggtg cgatccgcct
     2101 gctgggccac gtccgcacct ggggctggct gttcaacccc ctcaccatct acgtctgcac
     2161 cgacgccggc ggcgatcgca tcgaggccct ggtcctcgag gtgaccaaca ccccctggca
     2221 cgagcgccac gcctacgtcc tcgacggcgg ggcgggcgag caccgcttcg ccaaggcgct
     2281 gcacgtgtcc ccgttcttcg ggatggacca ggagtaccgg ctgcgcatgg gggcgccggg
     2341 cgagcacctc gtggtcgagc tcgcctccct cgagggcggc gccgtggtgt tcgaggcgag
     2401 cctggccctg cgtcgacggg cgatcacccg gtcgtccctc ggccggctcc tgtggcgcca
     2461 tcccctgctc acgctgcggg tctccaccgc catctacacc caggcgctgc gcctctgggc
     2521 caagcggacg ccgtaccacc cccacccggc ccggtcatga cctcccc
//
LOCUS       DASTFV010000174         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_625395, whole genome shotgun sequence.
ACCESSION   DASTFV010000174 DASTFV010000000
VERSION     DASTFV010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625395"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFP02_03290"
     CDS             <1..246
                     /locus_tag="VFP02_03290"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HET9542078.1"
                     /translation="DAACAAIAKAVGIDAKLVEFHVSAVTGGTDALGDVTVQVEHEGV
                     RVTGRGVATDVVEASARAYLQAVNKLTRSTERKEVPA"
     gene            243..1472
                     /locus_tag="VFP02_03295"
     CDS             243..1472
                     /locus_tag="VFP02_03295"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET9542079.1"
                     /translation="MTRRPNQVRPEEAHAAMARVFGVDVEADAASSSSTAPEPPAEES
                     EGRAALGRVFGTEAELSSRGYRIAVLAGDGIGPEVVAEARKAVEAAGARFGFGLDWVD
                     YDLGADRWLATGEALPDAVLEELREVDAILLGAVGRPDVPPGVLERGLLLRLRFELDL
                     YVNFRPVKLYPGVASPLADVKPDDIDLLVVRENTEGVYVGAGGTVHRGGPAEAATETS
                     IETRAGVERCLRFAAAKARERAGRLTLVHKTNVLVHAGDLWQRAARAVADETGVALDY
                     AHADAACLYLVSDPGRFDVIVTDNLFGDILTDLGAAIAGGLGTAASANLNPESSPSLF
                     EPVHGSAPDIAGTGRANPVAAILSASLLLDHLGEREAAAAVEAGAVAALPGAAGYSTQ
                     ALGDRIATAVAGGGSPD"
     gene            1486..>2073
                     /gene="ilvE"
                     /locus_tag="VFP02_03300"
     CDS             1486..>2073
                     /gene="ilvE"
                     /locus_tag="VFP02_03300"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HET9542080.1"
                     /translation="MPITPSGKIWMDGEYVDWDDAKIHVLTPSLHYGWGVFEGIRAYA
                     TDAGPAVFRLTDHMKRLYRSAKIYHMEPPVSLEGAIKVTKDLVAMNDLPACYIRPLVF
                     LGYGEIGLNPLTSPISMVIACWPWGAYLGEDAFQTGVRAKVSSWRRLDPNIIPPAAKA
                     TGQYINSSLAKAEAVKAGYDEGILLNPNGYVTDGSG"
BASE COUNT          302 a          768 c          743 g          260 t
ORIGIN      
        1 gacgccgcct gcgcggccat cgccaaggcg gtcgggatcg acgccaagct ggtcgagttc
       61 cacgtctcgg cggtcaccgg cggcaccgac gccctcgggg acgtgaccgt ccaggtggag
      121 cacgaggggg tgcgggtaac cgggcgcggc gtggccaccg acgtggtcga ggcctcggcg
      181 cgcgcctacc tccaggccgt caacaagctc acccgcagca ccgaacggaa ggaagtgccg
      241 gcatgaccag gcgacccaac caggtccgtc ccgaggaggc gcacgccgcc atggcccggg
      301 tcttcggcgt ggacgtggag gccgacgcgg cgtcgtcgtc gtccacggcg cccgagccgc
      361 cggccgagga gtccgagggc cgcgccgccc tcggccgcgt cttcggcacg gaggccgagt
      421 tgagctcacg tggataccgg atcgccgtcc tggccggcga cgggatcggg ccggaggtcg
      481 tggccgaggc gcgcaaggcc gtcgaggcgg ccggggcacg cttcggcttc ggcctggact
      541 gggtcgacta cgacctcggg gccgaccgct ggctcgccac cggggaggcc ctgcccgacg
      601 ccgtcctcga ggagctgcgc gaggtcgacg cgatcctgct cggggccgtc ggccggcccg
      661 acgtgccccc tggcgtgctg gagcggggcc tgctgctccg gctccgcttc gagctcgacc
      721 tctacgtgaa cttcaggccg gtgaagctgt acccgggggt ggcgtcgccg ctggccgacg
      781 tcaagcccga cgacatcgac ctgctggtgg tgcgggagaa caccgagggc gtgtacgtgg
      841 gcgccggcgg gaccgtccac cgcggcggcc cggccgaggc cgccaccgag acctcgatcg
      901 agaccagggc cggggtcgag cgctgcctgc gcttcgcggc cgccaaggcc cgcgagcggg
      961 cgggccggct caccctggtc cacaagacca acgtgctggt ccacgccggc gacctctggc
     1021 agcgggcggc cagggcggtg gccgacgaga ccggggtcgc cctcgactac gcccacgccg
     1081 acgccgcctg cctgtacctg gtcagcgacc cgggacgctt cgacgtgatc gtcaccgaca
     1141 acctgttcgg cgacatcctc accgacctgg gcgcggccat cgccggcggc ctcggcaccg
     1201 ccgccagcgc caacctcaac cccgaatcgt ccccctcctt gttcgaacca gtgcacgggt
     1261 cggcgcccga catcgccggg accggccggg ccaacccggt cgccgccata ctctcggcct
     1321 ccctgctcct ggaccatctg ggggagcggg aggccgcggc cgcggtcgaa gccggggccg
     1381 tagcggccct cccaggggct gcgggatact cgacccaggc gctcggggac cggatcgcca
     1441 ccgccgtcgc gggcggcggg tccccagact aggaggaggg cgacgatgcc gatcacacca
     1501 agcggcaaga tctggatgga cggggagtac gtcgactggg acgacgccaa gatccacgtg
     1561 ctgaccccgt cgctgcacta cggctggggc gtgttcgagg gcatccgcgc gtacgccacc
     1621 gacgccggcc cggccgtgtt ccggctcacc gaccacatga agcggctcta ccggagcgcc
     1681 aagatctacc acatggagcc gccggtctcc ctcgaggggg ccatcaaggt gaccaaggac
     1741 ctggtcgcca tgaacgacct gccggcctgc tacatccgcc cgctggtctt cctcggctac
     1801 ggcgagatcg gcctcaaccc gctcacctcg ccgatcagca tggtcatcgc ctgctggccg
     1861 tggggcgcct acctgggcga ggacgcgttc cagaccgggg tgcgggccaa ggtcagctcc
     1921 tggcggcggc tggaccccaa catcatcccg cccgcggcca aggcgaccgg ccagtacatc
     1981 aactcctcgc tggccaaggc cgaggcggtc aaggccggct acgacgaggg catcctgctc
     2041 aaccccaacg ggtacgtgac cgacgggtcg ggc
//
LOCUS       DASTFV010000175         4660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_627899, whole genome shotgun sequence.
ACCESSION   DASTFV010000175 DASTFV010000000
VERSION     DASTFV010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627899"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VFP02_03305"
     CDS             <1..639
                     /locus_tag="VFP02_03305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="involved in translesion DNA polymerization with
                     beta clamp of polymerase III; belongs to Y family of
                     polymerases; does not contain proofreading function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET9542081.1"
                     /translation="TAEKLRSRGIRSVGEVARLDEGSLVAMLGPASGRHLHALAHNRD
                     PRPVQVGRRRRSIGSQCALGWRPKTAADIDATLVAIVDRVTRRMRKADRVGRTVTLRL
                     RFDDFSRATRSHTLERATAHTPTVLATVRGLLVEAGSLVEERGGLTLVGVAVGNLDDR
                     GFVQLTLPFDRFSGSALDAVLDDVHERYGHDAVRRAVLLGRSTGLRVPMLPD"
     gene            672..2117
                     /locus_tag="VFP02_03310"
     CDS             672..2117
                     /locus_tag="VFP02_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET9542082.1"
                     /translation="MAPGRDGRRPRRTWPQRLLITFNIAMVMATLGLAAALGYANDKL
                     EQVQHLDLGTGVLDPAAEDPGEPQNYLLVGTDSAARLDESDPAARADEGVLSDTIMVL
                     RVDPDETQAQLLSFPRDLWVNIPDWGESKINAALSAGRDSLIATIQENFGIPINHYVE
                     VDFLGFQELVEAVDGVPIYFDKPLRDTHTGLNVTSTGCVTLSPDQALAYARSRYLQYY
                     EDGGWYYDGTGDFGRISRQQDFIRRTIQRAIDKGVRNPLTLNTLINAGISSVSLDDTL
                     VVDDLLQLGRRFRSFDPEQLRTMALDVYLDSVGEISIVRMVDNEANQARLNIFRGVAG
                     PDADAGAVDAASVGITTLNGTGTGGQATEVAAAFANLGFDTSPGIDDAERFDFAQTVI
                     RYKAGDEANAQFVASQLVAGAQLEQVADTGLANVQVITGLDFAGVKPTLVPPTTAAPS
                     PSTPSPTTTLPDLPTTTVLGEVPQAPEGESC"
     gene            complement(2118..2912)
                     /locus_tag="VFP02_03315"
     CDS             complement(2118..2912)
                     /locus_tag="VFP02_03315"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9542083.1"
                     /translation="MSLTASSPRVGELLRDWRRRRHLSQLDLSIEAEVSSRHLSFVET
                     GRSRPSRELLLHLAEHLEVPLRERNALLLAAGFAPVYRETPMDAEEMAPVRAALDKIL
                     AGHEPFPAVAVDSRWDLISANGPALGLLTAGVAPDLLDPPVNAMRVTLHPDGLAPRIA
                     NFAEYSAHLLLRLHRQALLTQDLVIADLLDELRGYPGVVEHTSAAVEPVDLLFSPLRI
                     EAPDGGELVFFSTLATFGTAVDITLAELSIEQFFPADAATAAALLG"
     gene            3002..4018
                     /gene="ligD"
                     /locus_tag="VFP02_03320"
     CDS             3002..4018
                     /gene="ligD"
                     /locus_tag="VFP02_03320"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET9542084.1"
                     /translation="MAAKDVVELEVAGHTVRITSPDKVFFAERGETKLDLVRYYEAVA
                     EPLMRTMAGRPTLMQRFPDGASGKSFFQKRVPSGAPEWLTTMVVSTPNGTTSNALVSE
                     DLAHILWAVNMGCLGFHTWPYHRDDPDHADELRIDLDPQPGIVFDHLREAASEVKALF
                     DELGVEAHPKTTGNRGLHIYVRLLPQWDSYEVRACAVAVARELERRRPDLVTAAWWKE
                     ERGERVFIDFNQNAPHKTVFSAWSARSRVGAQVSTPLFWDEIATVLPDELTIATVPAR
                     VAERGDPWGDMPDRPQSLEPLLALHEADMAAGLMDAPWPPVYPKQPNEPPRVAPSRAR
                     QDDG"
     gene            complement(4028..>4660)
                     /locus_tag="VFP02_03325"
     CDS             complement(4028..>4660)
                     /locus_tag="VFP02_03325"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9542085.1"
                     /translation="AVPPHRSPRSGPGVSETGEIIVVRHGQTAANAQGLLLGRLDVDL
                     DAVGRVQAVALAAALPAPDLVVTSPLARTRQTAAAFGEHIEVEVDEQWIEMDYGAFDG
                     RPVADVPAETWARWRADVDFAPPGGESLAGMTARVHDALDDLATRAAGRRVVVVTHVS
                     PIKSTVGWALQLGPEVSWRTFVAPASITRVGLGPNGPTLRSFNETMHLSA"
BASE COUNT          713 a         1725 c         1585 g          637 t
ORIGIN      
        1 acggcggaga agctccggtc gcggggcatc cggtcggtcg gcgaggtggc ccggctggac
       61 gagggctcac tcgtcgcgat gctcggaccg gcgtccgggc gtcacctgca cgctttggcc
      121 cacaaccgtg atccccgtcc ggtgcaggtc gggcgccgac ggcgctcgat cggctcacag
      181 tgcgccctcg ggtggcgccc caagaccgcg gcggacatcg acgccacgct cgtcgccatc
      241 gtcgaccggg tgacccgcag gatgcgcaag gccgaccgcg tggggcgcac cgtgaccctc
      301 cgcctccgct tcgacgactt cagccgggcc acccgctccc acacgctgga gcgggccacc
      361 gcccacacgc cgaccgtcct cgccacggtg cggggcctcc tggtcgaggc cggctccctg
      421 gtcgaggaac gaggcgggct cacgctggtg ggcgtggcgg tgggcaacct cgatgaccgg
      481 ggattcgtgc aactcaccct gcccttcgac cggttcagcg ggtcggccct ggacgccgtc
      541 ctcgacgacg tgcacgagcg ctacggccac gacgccgtcc gtcgggccgt gctcctcggg
      601 cgctcgaccg ggctcagggt gccgatgctg cccgactgat ctcggcctcg agtcgtcggt
      661 agcctcaccc gatggctcca ggaagggacg gcaggcgtcc acggcgtacg tggcctcagc
      721 gcctgctgat caccttcaac atcgccatgg tcatggccac cctgggccta gccgcggccc
      781 tcggctacgc caacgacaag ctggagcagg tccagcacct cgatctcggt accggcgtcc
      841 tcgaccccgc cgccgaagac ccgggcgagc cccagaacta cctgctggtg ggcacggaca
      901 gcgcggcccg cctcgatgag agtgaccccg ccgcccgcgc ggacgagggt gtcctctcgg
      961 acacgatcat ggtgctgcgg gtcgaccccg acgagaccca ggcccagttg ctgtccttcc
     1021 cccgtgacct ctgggtgaac atccccgact ggggtgagtc gaagatcaac gccgccctct
     1081 cggccgggcg cgactcgctc atcgccacca tccaggagaa cttcgggatc cccatcaacc
     1141 actatgtcga ggtcgacttc ctggggttcc aggagttggt cgaggccgtc gacggcgtcc
     1201 cgatctactt cgacaagccg ctgcgggaca cccacacggg cctcaacgtc accagcaccg
     1261 gttgcgtcac gttgagcccc gaccaggcgc tggcctacgc ccgctcccgg tacctgcagt
     1321 actacgaaga cggcggttgg tactacgacg ggaccggcga cttcggtcgt atctcccgcc
     1381 agcaggactt cattcgcagg acgatccagc gggccatcga caagggcgtc cggaaccccc
     1441 tcaccctcaa cacgctcatc aacgccggca tcagcagcgt gtccctcgac gacaccctgg
     1501 tcgtcgacga cctgctccag ctgggccgcc gcttccggtc cttcgacccc gagcagctca
     1561 ggacgatggc gctggacgtc tacctcgact ccgtcggcga gatcagcatc gtgcgcatgg
     1621 tcgacaacga ggccaatcag gcccggctca acatcttccg gggcgtcgcc ggccccgacg
     1681 ccgacgccgg ggcggtcgat gccgcttccg tcgggatcac gacgctcaac ggcacgggca
     1741 ccggcggcca ggccaccgag gtggcggccg cgttcgccaa cctcggcttc gacacctcgc
     1801 ccggtatcga tgacgccgaa cgcttcgact tcgctcagac cgtgatccgc tacaaggccg
     1861 gtgacgaggc caacgcccag ttcgtggcca gccagctggt ggccggagcc cagctcgagc
     1921 aagtggccga cacgggcttg gccaacgtcc aggtcatcac cggcctcgac ttcgccggtg
     1981 tcaagcccac gctggtaccg ccgacgaccg cggccccctc gccgtccacg cccagcccga
     2041 ccaccacgct gcccgacctc ccgaccacga ccgtgctcgg cgaggtgccc caggcccccg
     2101 agggcgagtc ctgctgatca gcccaggagg gctgcggccg tggccgcgtc ggcggggaag
     2161 aactgttcga tcgacagctc ggccagggtg atgtccacgg cggtgccgaa ggtggcgagg
     2221 gtcgagaaga acaccagctc cccgccgtcg ggggcctcga tgcggagcgg cgagaagagc
     2281 aggtccaccg gctccaccgc ggcgctggtg tgctcgacca caccgggata gccccggagc
     2341 tcgtcgagga ggtcggcgat gaccagatcc tgggtgagca gggcctggcg gtgcaggcgg
     2401 agcaggaggt gggcgctgta ctccgcgaag ttggcgatgc gcggggccag gccgtcgggg
     2461 tgcagggtca cccgcatggc gttcaccggc gggtccagca ggtcgggggc cacgcccgcg
     2521 gtgaggagcc cgagagcagg cccgttggcg gagatcaggt cccaccggct gtcgacggcc
     2581 accgccggga agggctcgtg gccggcgagg atcttgtcca acgcggcgcg gaccggcgcc
     2641 atctcctcgg cgtccatcgg cgtctcccgg tacacggggg cgaacccggc cgccagcagc
     2701 agcgcgttgc gctcccgtag cgggacctcc aggtgctcgg ccaggtgcaa cagcagttcc
     2761 ctgctgggcc tggaccgccc ggtctcgacg aagctgagat gccgcgacga cacctccgcc
     2821 tcgatcgaca ggtcgagctg gctcagatgg cgccgtcggc gccagtcccg gagcagctcc
     2881 cccacccggg gcgacgaggc ggtgaggctc atggcgccga ccctaacgac gccctgatcc
     2941 gggagcgctt acccgtgggg taagcgccct cgttcccgcc ccagccccta ccgtgaggga
     3001 catggcggcc aaggacgtgg tcgagctgga ggtggccggg cacaccgtgc gcatcaccag
     3061 ccccgacaag gtcttcttcg ccgagcgggg cgagaccaag ctcgacctgg tccgctacta
     3121 cgaggccgtc gccgagcccc tgatgcgcac gatggccggt cgacccaccc tcatgcaacg
     3181 cttcccggac ggagcgagcg ggaagtcgtt cttccagaag cgggtcccca gcggcgcccc
     3241 cgaatggctg accacgatgg tggtgagcac gcccaacggc acgacgtcca acgccctcgt
     3301 gagcgaggac ctggcccaca tcctctgggc cgtcaacatg ggctgcctcg gcttccacac
     3361 ctggccgtac caccgcgacg accccgacca cgccgacgag ctgcgcatcg acctggaccc
     3421 gcagccgggc atcgtcttcg accacctgcg ggaggcggcg agcgaggtca aggcgctctt
     3481 cgacgagctc ggcgtcgagg cccaccccaa gacgacgggc aaccggggcc tgcacatcta
     3541 tgtacggctg ctgccccagt gggacagcta cgaggtgcgg gcctgcgcgg tggccgtggc
     3601 ccgcgagctg gaacgtcgac gcccggatct ggtcaccgcg gcgtggtgga aggaggagcg
     3661 gggcgagcgg gtgttcatcg acttcaacca gaacgccccc cacaagaccg tcttcagcgc
     3721 ctggtccgcc cgatcacggg tcggcgctca ggtctccacc cccttgttct gggacgagat
     3781 cgccacggtc cttcccgacg agctgaccat cgccaccgtc cccgcccgcg tggccgagcg
     3841 gggcgatccg tggggcgaca tgccggaccg gccgcagtcc ctcgagcccc tgctcgcgct
     3901 ccacgaggcc gacatggctg ccgggctgat ggatgcccct tggccgcccg tctatcccaa
     3961 gcaacccaac gagccgcctc gggtcgcgcc gagccgggcc cgccaggacg acggttgaac
     4021 gggcgcgtca cgcgctcagg tgcatcgtct cgttgaagga ccggagggtg ggcccgttcg
     4081 gccccaaccc gacccgggtg atggaggccg gggcgacgaa ggtccgccat gacacctccg
     4141 gcccgagctg gagggcccag ccgacggtcg acttgatcgg cgacacatgg gtgaccacca
     4201 ccacccggcg gccggcggcc cttgtcgcga ggtcatcgag ggcgtcgtgg acccgggccg
     4261 tcatcccggc cagcgactct ccaccgggag gggcgaagtc gacatcggcc cgccagcggg
     4321 cccacgtctc ggccggcacg tccgccacgg ggcgcccgtc gaaggcgccg tagtccatct
     4381 cgatccactg ctcgtcgacc tcgacctcga tgtgctcgcc gaacgcggcc gccgtctgcc
     4441 gggtccgggc caacgggctg gtcaccacca ggtccggggc gggcagggcc gcggccaacg
     4501 ccaccgcctg cacgcggccg acggcatcga ggtccacgtc gaggcggccc aggagcaggc
     4561 cctgggcgtt ggccgcggtc tggccgtggc ggacgacgat gatttcgccg gtttcgctca
     4621 cgcccggtcc cgagcgaggt gaccggtgtg gcggaaccgc
//
LOCUS       DASTFV010000176         2593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_648796, whole genome shotgun sequence.
ACCESSION   DASTFV010000176 DASTFV010000000
VERSION     DASTFV010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648796"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(360..1115)
                     /locus_tag="VFP02_03330"
     CDS             complement(360..1115)
                     /locus_tag="VFP02_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542086.1"
                     /translation="MNASIKRPDVAMDTAREAESTDSRWRALYRLAGAGALLSAVFIP
                     IQVLVFIVWPPPLEGTAADWFALFRDNRLVGLLDLDLLLIADNLLLVPILLALYVLLR
                     RDSQSLMAIATVLGFLGIVFFIASNPAFEMLSLSQRYAAAATEAQRSTFLAAGEAMLA
                     TWQGTAFQTYYLLGSIAGIMIGAVMLRSAVFSNLTGWMAILGNSVGLGLYVPTVGVYI
                     SVFSVLFLEVWYVLIARRLLQLGRRASEVEASP"
     gene            complement(1215..1649)
                     /locus_tag="VFP02_03335"
     CDS             complement(1215..1649)
                     /locus_tag="VFP02_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542087.1"
                     /translation="MVISAIGPPCRPEEAWHGCEPAFTLIPDFGLSGLVAMAVASVVL
                     LWSVAFVHRRHGELVLLLSAILFLVGGGFSTLWFGILAGIAGTRIGGPLPWWRARVPP
                     RLSRWSLARLWPWLLVTYLGWWAGSWAIAAFAADLMLRLPPP"
     gene            complement(1679..2182)
                     /locus_tag="VFP02_03340"
     CDS             complement(1679..2182)
                     /locus_tag="VFP02_03340"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9542088.1"
                     /translation="MVHIKGEIVIDRPVEEVFDFVADERNEPRYNPRMRGVEKLSTGP
                     IGVGTRFRAETTSTGRTVPLVIEFTGYDRPRRLASTTHMSSMNLQGALTFDSVSGGTR
                     MRWAWDVEPRGLLKLMRPLVALMGRRQEQGIWRGLKHLLEAGEMPGRRPDEVTMKPDW
                     QTNDERA"
     gene            complement(2237..>2593)
                     /locus_tag="VFP02_03345"
     CDS             complement(2237..>2593)
                     /locus_tag="VFP02_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542089.1"
                     /translation="LLSVFLLLVGGGFGPPLVGIIIGIAATRIGVAGRRRPGSVSRAL
                     GRAWPWLLGAGVLGYLSLVPGAIVLDWLIEADIAGLVAGLMVLSFTSLILALVAARAR
                     DRLRTGQTADPGEMAR"
BASE COUNT          466 a          897 c          881 g          349 t
ORIGIN      
        1 accgggcgct cgggcttgca gcgccagatc cacccgccgg actgaccgtc ggtcggcgag
       61 gatcggcaag acacggtgcg tcgacgtgac ctgctcggcg gtctgctcca cgagtaccgg
      121 cgagctgcag gaacacgttt gcgcaccctt cacccagggc tccggcgttc tcttgatatg
      181 cccgatcgtg cgatagcgcc cgataagtgt ggagcaggca cgaacctgcg ggatcatgcg
      241 gtcccaacgc ctcatgaccg ggccagggct atctcaccca ctggagcgag cccggtgggc
      301 cacggccctg gcaaccgctt cggggttgcc gtacaccgac tcgtagagga cgacggcctt
      361 catggagatg cctccacctc ggatgcgcgc ctccccaact ggagcagccg tcgggcaatc
      421 aggacgtacc agacctccag gaataggacg gagaacaccg agatgtagac cccgacggtg
      481 gggacgtaga ggcccaggcc aacggagttc cccaggatgg ccatccaccc ggtcaggttg
      541 ctgaacacgg cgcttcggag catcaccgcc ccgatcatga tgccggcgat ggaaccgagg
      601 aggtagtagg tctggaaggc ggtgccctgc caggtggcga gcatggcctc ccccgccgcc
      661 aggaaggtcg atcgctgggc ctcggtggcg gccgcggcgt accgctggct cagagagagc
      721 atctcgaacg ccgggttcga ggcgatgaag aacacgatcc ccaggaaccc gaggaccgtg
      781 gctatggcca tcagggattg gctgtcccgc cggagcagca cgtatagggc caggaggatc
      841 ggcaccagga gcaggttgtc ggcaatcagc aggaggtcca ggtcgaggag ccccaccagc
      901 cggttgtcgc ggaacagggc gaaccagtcc gcggcggttc cttcgagcgg tggcggccag
      961 acgatgaaca ccaggacctg gatcgggatg aataccgccg agagcagggc gcccgccccg
     1021 gcgagccggt acagggccct ccagcgcgag tccgtggact cggcttctcg ggctgtgtcc
     1081 atggcgacgt caggccgctt gatggatgcg ttcacctggg acctcctctt ggttggggac
     1141 acctgcccgg ggccgggtag cgtgcgcgag ggcagacagc aggatcagcg cgaggaccac
     1201 gggcgtggcg atggtcacgg cggggggagc cgcagcatca ggtcggccgc gaaggcggcg
     1261 atggcccacg acccggccca ccaccccagg taggtcacca ggagccacgg ccacagccga
     1321 gccagcgacc accgcgacag gcggggcggc acacgggccc gccaccaggg gagcggcccg
     1381 ccaatccggg tgccggcgat cccggccagg atcccaaacc acagtgtgct gaagccgcca
     1441 ccgacgagga acaggatcgc cgacagcagc aggaccagtt cgccgtggcg ccggtggacg
     1501 aaggccacgg accacagcag gaccacgctg gcgaccgcca tggcaacgag gccgctgagg
     1561 ccgaagtccg ggatcagggt gaacgcgggc tcgcagccgt gccacgcctc ctccggccgg
     1621 cacggcggcc cgatggcgct gattaccaag cccgcacggc gctgcgaata cgaggatttc
     1681 aggctcgttc atcgttcgtc tgccaatccg gcttcatcgt gacctcatcc ggcctgcggc
     1741 ctggcatctc acctgcttcc aggagatgct tgaggcccct ccagatcccc tgttcctgac
     1801 gccgacccat gagggcgacc agcggccgca tgagcttgag gaggccgcgc ggctccacgt
     1861 cccacgccca ccgcatccga gtccctccgg acaccgagtc gaacgtcagg gcgccctgca
     1921 ggttcatgga ggacatgtgg gtggtcgagg ccagccgccg gggtcggtcg tagccggtga
     1981 actcgatgac cagggggacg gtccggcccg tgctcgtggt ctccgcccgg aaccgggtgc
     2041 cgacgccgat cggtccggtc gagagcttct cgaccccgcg catccggggg ttgtagcggg
     2101 gctcgttgcg ctcgtcggcg acgaagtcga agacctcctc caccggccgg tcgatgacga
     2161 tctcgccctt gatgtgcacc atgtcgttca cctcctcaac ctgttccgtc tcacatccgt
     2221 ccgcccgaac cgatcctcat ctggccatct cccccgggtc tgccgtctgg ccggttcgca
     2281 ggcggtcccg ggcccgggcg gccaccaggg ccaagatgag cgaggtgaag gaaaggacca
     2341 tgagcccggc cacaagcccc gcgatatccg cttcgatcag ccaatcgagg acgatcgcgc
     2401 ctggcaccag cgacaggtag ccgagcaccc cggctcccag gagccatggc caggcccgcc
     2461 ccagagcccg ggagacactc cccgggcgcc ggcggccggc gaccccgatc ctggtggcag
     2521 cgatgccgat gatgatcccg accagcggcg gcccgaagcc cccgcccaca aggagcagga
     2581 acaccgacag caa
//
LOCUS       DASTFV010000177         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_649428, whole genome shotgun sequence.
ACCESSION   DASTFV010000177 DASTFV010000000
VERSION     DASTFV010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649428"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VFP02_03350"
     CDS             <1..942
                     /locus_tag="VFP02_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET9542090.1"
                     /translation="EVRFGRKGDYFETVGYLDVREADKRFEEAEQRRLLYVAATRAQD
                     HLVVSLHHKAGYPSHAALLHEVCQAKAGPVTPPEDIQLALPLTVDARVPTERPVEGQE
                     VAEDWRRARLELLGRVRGSGVATPSGLGHDEPLPVPEEDQRVPGRGEGGTARGRAVHA
                     VLQAVTLPDGPDLDDLSRLEAAAEGLEDPAAVAALARSALAAPVVQRALAAPRHWREL
                     AVQAEIRGRLVEGFIDLAFEDDDGRLVVIDYKTDAAVNPAAYRLQIGAYAAALARATG
                     REVAEGWLVFATEDGAEERQVTDLAAAVTEVEDLVAG"
     gene            complement(944..2101)
                     /locus_tag="VFP02_03355"
     CDS             complement(944..2101)
                     /locus_tag="VFP02_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9542091.1"
                     /translation="MGLGATMALGSSQGRSRLPARQALVGAAVGVLGVVATITLNAGL
                     TDALDHPERAGVAWGAGGIVSGEGLQPDAVSPELLETIAATPGVAAIATIDRQVLPVD
                     GVGVPTFAVRPTPGADVSPIDLVLTSGRAPEGTDEAAIGPASAADLGVGVGDEVTVGA
                     TDQQVSIVGEALFPTDVHATFDEGLWLDGEGFDRAAPAELNEVEGVQRQVAIEVADGA
                     SEEEVVAALQASVGDQFQDLGPAVTPVELSNLENVRMLPRVLAGFLALLAVGALGHVL
                     ATAARRRRQDFAVLRALGLTRWDSRVVLNVQGTVIGLVGLLVGIPAGIVLGQLGWAVV
                     AESVPLAVVRPVAALAVAALIPLALLVANGLAVWPGRRVARLRPGEVLRTE"
BASE COUNT          301 a          816 c          859 g          242 t
ORIGIN      
        1 gaggtccgct tcgggcggaa gggtgactac ttcgagacgg tcggctacct cgacgtccgc
       61 gaggccgaca agcgcttcga ggaggccgag cagcgccggc tgctgtacgt cgccgccacc
      121 cgagcgcagg accacctcgt cgtgagcctc caccacaagg cgggctaccc gagccacgcc
      181 gccctgctcc acgaggtctg ccaggccaag gccgggcctg tgaccccgcc cgaggacatc
      241 cagctcgccc tgcccctgac cgtggacgcc agagtcccga ccgagcgacc cgtggagggc
      301 caggaggtgg cggaggactg gcggcgggcc cggctcgagc tcctgggccg ggtccggggc
      361 tcgggggtgg cgacgccgag cggcctcggc cacgacgagc cactgcccgt ccccgaggag
      421 gaccagcgcg tccccgggcg gggcgagggg ggcacggccc gcggtcgcgc cgtccacgcc
      481 gtcctgcagg ccgtgaccct gcccgacgga cctgacctgg atgacctgag ccggctcgag
      541 gcggccgccg agggcttgga ggacccggct gcggtggctg ctctcgcccg gtcggccctc
      601 gccgccccgg tcgtgcagcg ggccctggcc gcgccccgcc actggcggga gctggccgtg
      661 caggccgaga tccgcggccg cctggtcgaa gggttcatcg acctcgcctt cgaggacgac
      721 gacggccgcc tcgtggtcat cgactacaag accgacgccg ccgtgaaccc cgccgcctac
      781 cggctccaga tcggggccta cgccgccgcc ctggcccggg cgacgggtcg cgaggtcgcc
      841 gagggctggc tggtcttcgc caccgaggac ggggccgaag agcggcaggt gaccgatctc
      901 gccgcggcgg tgaccgaggt cgaggacctc gtcgccggct gactcactcg gtgcgcagga
      961 cctcgccggg acggaggcgg gcgactcggc ggccgggcca cacggcgagg ccgttggcga
     1021 cgaggagggc gagcgggatc agcgcggcta cggctagagc ggcgacgggc cgcaccacgg
     1081 cgagcggcac gctctcggcc acgaccgccc agcccagctg cccgaggacg atcccggcgg
     1141 ggatgcccac cagcaggccg accagcccga tgaccgtgcc ctgcacgttg agcacgaccc
     1201 gggagtccca ccgggtcagg ccgagggcac ggagcacggc gaagtcctgc cggcgacgcc
     1261 gggccgcggt ggcgaggacg tggccgaggg cgcccaccgc cagcaaggcg aggaacccgg
     1321 ccagcacccg cggcagcatg cgcacgttct cgaggttgct gagctccact ggcgtgacgg
     1381 ccggccccag gtcctggaac tggtccccga cggaagcctg gagggcggcg acgacctcct
     1441 cctcggacgc gccgtcggcg acctcgatgg cgacctgccg ctggacgcct tccacctcgt
     1501 tgagctcggc cggtgcggct cggtcgaagc cttcgccgtc gagccagagg ccctcgtcga
     1561 acgtggcgtg gacgtcggtc gggaagaggg cctcgcccac gatcgacacc tgctggtcgg
     1621 tcgccccgac cgtgacctcg tcgcccacgc cgacgccgag gtcggcggcg gaggccggcc
     1681 cgatggcggc ctcgtcggtg ccctccgggg cccgtcccga ggtcaggacg aggtcgatcg
     1741 gcgacacgtc ggccccgggg gtcgggcgga cggcaaaggt cggcaccccg acgccgtcca
     1801 cggggaggac ctgacggtcg atcgtcgcta tcgcggcgac gccgggcgtg gccgcgatcg
     1861 tctccaggag ctcgggggac acggcatcgg gctgcaaccc ctcgccggag acgatgccgc
     1921 cggcgcccca ggcgacgccg gcccgctcgg ggtgatccaa ggcgtcggtc agcccggcgt
     1981 tgagggtgat ggtggccacc accccgagga cgccgacggc cgcgcccacc agcgcctggc
     2041 gggcggggag gcggctcctg ccctggctgg agccgagggc catcgtcgcg ccgagtccca
     2101 cgggaacggg ggtcgcccgt cggatccagg cgagggccga ggagggtggt gaggagcggg
     2161 cgcgggtctg gcgtcggaac gaggtcgcgg tgcctgcacc gacgagcacg gccagggc
//
LOCUS       DASTFV010000178         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_651039, whole genome shotgun sequence.
ACCESSION   DASTFV010000178 DASTFV010000000
VERSION     DASTFV010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651039"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VFP02_03360"
     CDS             complement(<1..970)
                     /locus_tag="VFP02_03360"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HET9542092.1"
                     /translation="MAPSTDLTTDERSDLDAARQGDEAAFTRLVEAHRRPLHAHCYRM
                     LGSVHDADDALQDTLLAAWRGLRGFAGTGSFRSWLYRIATNASIRVGERRRRRMLVPE
                     LGPAFTQVWDLGETVTEPVFIEPYPDAGLADRSGTTDPEARYDLLESVELGFVAALQA
                     LPATQRAVLVLRDVLAIPAAEVAETLDTTVAAVNSALQRARKTMEDRVDPVTQQATRR
                     ALGEDGEQQLVHSFIRAWEHHDVDGLVGLLAEDARLTMPPMPAWFTGRAAIGEFFADR
                     AFDRPWRFVPITASGQLAVVGYMGDDAGAYPLQNVNVLTLRGRDIVA"
     gene            complement(1023..2012)
                     /locus_tag="VFP02_03365"
     CDS             complement(1023..2012)
                     /locus_tag="VFP02_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET9542093.1"
                     /translation="MTPPHPVRFGAFVPQGWKLEFTGWDPARAWARAKEVALAAELAG
                     YDHLWVYDHVETVPRREPEPMFEAWSMMAALAEVTRTARLGQLVTCAGYRNPALLAKQ
                     AATVDVMSGGRLIFGIGAGWYDEEYRSHGWTYPPDRQRLELLEETIQIAKAMWGEPSA
                     GLHGHHAWVDDAISFPKPIQSPLPIWVGGGGEKVTLRIAAEHAHATNWQVGLEEFVHK
                     SEVLQGHCAAVGRDFDDVVRTHAPDCRLFDTEADLARWLDEPEGGDLWGEEDPATYVR
                     DNFVGTDAQVAEKVQAFLDAGCREFVLWFRDYPDTESMERFMAAVVPRLHLSR"
     gene            complement(2009..>2317)
                     /locus_tag="VFP02_03370"
     CDS             complement(2009..>2317)
                     /locus_tag="VFP02_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542094.1"
                     /translation="AYDWEQFLFLIADDDPMACPRLDHFGMSVSSLDELEAFLAKAKA
                     FAEQDDRVDIIDQKAEVHGDALTLHSFYVRYLLPMMIEIQFFDWDPSVAALNPANQPA
                     "
BASE COUNT          343 a          773 c          866 g          335 t
ORIGIN      
        1 cggccacgat gtcccgaccc cggagggtga ggacgttgac gttctggagt gggtaggcac
       61 cggcgtcgtc gcccatgtag ccgacgaccg ccaactggcc gctcgcggtg atgggcacga
      121 accgccacgg tcggtcgaag gcacgatcgg cgaagaactc gccgatggcg gcccggcccg
      181 tgaaccaggc gggcatcggg ggcatcgtga gccgggcgtc ctcggccagc agccccacga
      241 gcccgtcgac gtcgtggtgc tcccaggccc ggatgaagga gtgcacgagt tgctgctcgc
      301 cgtcctcgcc gagggcccgg cgcgtggctt gctgggtcac cgggtcgaca cggtcctcca
      361 tcgtcttccg ggcccgttgc agggcgctgt tgaccgcggc aaccgtcgtg tcgagggtct
      421 ccgccacctc cgcggcgggg atggccagga catcgcgcag cacgaggacc gcccgctggg
      481 tggcgggcag ggcctgcagc gcggcgacga agcccaactc gacgctctcc agcaggtcgt
      541 agcgggcctc ggggtcggtg gtgccggacc gatcggccag cccagcgtcg gggtagggct
      601 cgatgaagac cggctcggtc accgtctcgc cgaggtccca cacctgggtg aacgcggggc
      661 cgagctcggg caccaacatc cggcgccggc ggcgctcgcc gacccggatt gacgcgttgg
      721 tggcgattcg gtagagccag gaccggaacg agccggttcc ggcgaaccca cgaaggcctc
      781 tccaagcggc caggagcgtg tcctgcagcg cgtcgtcggc gtcgtgcacc gagcccagca
      841 tccggtagca gtgggcgtgc agtggccgcc ggtgggcctc gacgagccgg gtgaaggcgg
      901 cctcgtcgcc ttggcgggcg gcgtcgaggt cggagcgctc gtcggtcgtg agatcggtcg
      961 aaggggccaa gcgtcgaacc tacccagggt cgagcgttcc gcggaacgtc cggcgccgtc
     1021 gctcagcgcg agaggtgcag cctcgggacg acggcggcca tgaagcgctc catggactcg
     1081 gtgtcggggt agtcacggaa ccagaggacg aactcgcggc agccggcgtc gaggaaggcc
     1141 tggaccttct cggcgacctg ggcgtcggtg ccgacgaagt tgtcccgaac gtaggtcgcg
     1201 gggtcctcct cgccccagag gtcgccgcct tcgggctcgt ccagccagcg cgccagatcg
     1261 gcctcggtgt cgaagagacg gcagtccggg gcgtgggtgc ggacgacgtc gtcgaagtcc
     1321 cggcccacgg cagcacagtg gccctgcagg acctccgact tgtgcacgaa ctcctcgaga
     1381 cccacctgcc agttggtggc gtgcgcgtgt tcggcggcga tgcgcagggt gaccttctcg
     1441 ccgccgccgc cgacccagat gggcagaggt gactggatgg gtttggggaa cgagatggcg
     1501 tcgtcgaccc aggcgtggtg cccgtgcagg ccggccgacg gctcgcccca catggccttc
     1561 gcgatctgga tggtctcctc cagcagctcc agccgctggc ggtcgggggg ataggtccat
     1621 ccgtgggacc ggtactcctc gtcgtaccag cccgccccga tgccgaagat gagccggccg
     1681 ccggacatga cgtcgaccgt ggcggcctgc ttggccaaca gggcggggtt gcggtagccg
     1741 gcgcaggtga cgagctggcc cagtcgggcc gtccgggtga cctcggccag cgccgccatc
     1801 atcgaccagg cctcgaacat cggctccggt tcccggcggg ggacggtctc gacgtggtcg
     1861 tagacccaga ggtggtcgta gcccgccagc tccgcggcca acgccacctc cttggcccgg
     1921 gcccaggccc gggcggggtc ccagcccgtg aactccagct tccagccctg cgggacgaac
     1981 gccccgaagc ggaccggatg gggcggggtc acgcgggctg gttggccggg ttgagcgcgg
     2041 cgaccgacgg atcccagtcg aagaactgga tctcgatcat catcggaagc aggtagcgca
     2101 cgtagaagct gtgcagggtg agggcgtcgc cgtggacctc ggccttctga tcgatgatgt
     2161 cgacccggtc gtcctgctcg gcgaaggcct tcgccttggc caggaacgcc tcgagctcgt
     2221 cgagcgagct caccgacatc ccgaagtggt cgagacgggg gcacgccatc gggtcgtcgt
     2281 cggcgatgag gaagaggaac tgctcccagt cgtaggc
//
LOCUS       DASTFV010000179         2180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_652793, whole genome shotgun sequence.
ACCESSION   DASTFV010000179 DASTFV010000000
VERSION     DASTFV010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652793"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VFP02_03375"
     CDS             complement(<1..1049)
                     /locus_tag="VFP02_03375"
                     /inference="COORDINATES: protein motif:HMM:NF005892.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HET9542095.1"
                     /translation="MSGDVGFVGTTAVAGIGATEFSKASGRSEMQLACEATVLALEDA
                     GIEPSEVDGMVTFTADTNPEIEIARLTGIGELTFFSRIHHGGGAACGTVQQAAMAVAE
                     GIADVVVVYRAFNERSGHRFGTGVQDRPPAANAEAAHFAWYAPFGLLTPAQWVAMFGR
                     RYMHETGATSEDFGRVSVADRHHAATNPKAFFYEQPITLEDHQNSRWIVEPLHLLDCC
                     QESDGGQALVVTSLERARDLKTPPAVIVGAAQGSGEDQQMMTSYYRDSITGIPEMGLV
                     ARQLYEQAGIGPDDIQTAVLYDHFTPFVLCQLEEFGFCGKGEAKDFVRDGHLEMSGRL
                     PTNTHGGQLGEAYIHG"
     gene            complement(1046..1468)
                     /locus_tag="VFP02_03380"
     CDS             complement(1046..1468)
                     /locus_tag="VFP02_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET9542096.1"
                     /translation="MTDLPPSRAFADVAVGDELTPLAIPLTRTVIVATAIATRDYQDV
                     HHDPSLAVERGSPDIFMNILSTNGFVNRFVTEWAGPASRVTKLSIRLGAPNYPGDTMS
                     FTGSVEAKDDATNTVTVGLVGANSIGAHVTGKVELVLP"
     gene            complement(1461..>2180)
                     /locus_tag="VFP02_03385"
     CDS             complement(1461..>2180)
                     /locus_tag="VFP02_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9542097.1"
                     /translation="EVEPAGRGEAREVDGDWRLTGFWPSVPAGHLADAIVVPAVVDGS
                     VALFLVDPSAAGASVEVATTTNRSKVGHYTFDATPAEPLDGGADALAFAHERALVALA
                     AISLGACEEATRMAAEYTSGREQFGKPLSTNQGVVLKAADAYIALAAMRATLWQAAWR
                     LDEGLDAADAVEVAHWWASDGGHRVVHITQHLHGGMGADIDYPVHRYFLWAKEITDTL
                     GGPSAHLARLGDLIAAETDRD"
BASE COUNT          304 a          739 c          801 g          336 t
ORIGIN      
        1 ccgtggatgt aggcctcgcc caactgcccc ccgtgggtgt tggtgggcag gcgcccgctc
       61 atctccaggt gcccgtcccg cacgaagtcc ttggcctcgc ccttgccgca gaagccgaac
      121 tcctccagct ggcagagcac gaagggcgtg aagtggtcgt agaggacggc ggtttggatg
      181 tcgtcgggac cgatgccggc ctgctcgtag agctggcggg cgaccaggcc catctccggg
      241 atgccggtga tggagtcccg gtagtaggag gtcatcatct gctggtcctc ccccgagccc
      301 tgcgccgccc cgacgatgac cgccggaggc gtcttcaggt cccgagcccg ctccagggac
      361 gtgaccacga gggcttggcc cccgtcgctc tcctggcagc agtcgagcag gtgcagcggc
      421 tcgacgatcc accggctgtt ctggtggtcc tcgagggtga tcggctgctc gtagaagaag
      481 gccttcgggt tggtggcggc gtggtgccgg tcggcgacgg agacccgacc gaagtcctcg
      541 ctggtggccc cggtctcgtg catgtaccgc cggccgaaca tggccaccca ctgcgcgggg
      601 gtgagcaggc cgaagggggc gtaccaggcg aagtgggccg cttccgcgtt cgccgcgggc
      661 ggtcggtcct ggacgcccgt gccgaagcga tggccggagc gctcgttgaa ggcccggtag
      721 acgacgacca cgtcggcgat gccttcggcc accgccatgg ccgcctgctg gacggtgccg
      781 cacgccgctc cgccaccgtg gtggatccgg gagaagaacg tgagctcgcc gatgcccgtg
      841 aggcgggcga tctcgatctc ggggttggtg tcggcggtga aggtgaccat gccgtcgacc
      901 tcggacggct cgatgccggc gtcctcgagg gccaggaccg tggcctcgca ggccaactgc
      961 atctccgagc gccccgaggc cttggagaac tcggtggcgc cgatcccggc gaccgcggtg
     1021 gtcccgacga accccacatc gccgctcatg gcagcaccaa ctcgaccttg ccggtgacgt
     1081 gggcgccgat cgagttggcc ccgaccaggc cgaccgtgac cgtgttggtg gcgtcgtcct
     1141 tcgcctcgac cgacccggtg aagctcatgg tgtcccccgg gtagttgggg gctccgagcc
     1201 ggatcgacag cttggtcacc cggctggccg ggcccgccca ctcggtgacg aagcggttca
     1261 cgaagccgtt ggtggagagg atgttcatga agatgtcggg cgagccccgc tcgaccgcga
     1321 gcgagggatc gtggtgcacg tcctggtagt cccgggtggc gatcgccgtc gccacgatga
     1381 ccgtgcgggt cagcgggatg gccaacgggg tcagttcgtc gccgacggcc acgtcggcaa
     1441 aggcccggga gggagggagg tcagtcacgg tcggtctccg cggcgatgag gtcgcccagt
     1501 cgggcgaggt gggcgctggg gcccccgagg gtgtcggtga tctccttggc ccagaggaag
     1561 tagcgatgga cggggtagtc gatgtccgcg cccatgcccc cgtgcaggtg ttgggtgatg
     1621 tgcacgaccc ggtggccgcc gtcagaggcc caccagtggg cgacctcgac ggcgtcggcc
     1681 gcgtccagtc cctcgtcgag gcgccatgcc gcctgccaca acgtggcccg catcgccgcc
     1741 agcgcgatgt aggcgtcggc cgccttgagc accactccct ggttggtcga cagaggcttg
     1801 ccgaactgtt cccggcccga cgtgtactcg gccgccatcc gggtggcctc ctcacaggcc
     1861 cccagcgaga tggccgccag ggccaccagc gcccgctcgt gggcgaaggc cagggcatcg
     1921 gcgccgccgt cgaggggctc ggccggcgtc gcgtcgaagg tgtagtgacc gactttgctc
     1981 cgattggtcg tcgtcgccac ctcgaccgag gcgccggcgg ccgaagggtc caccaggaac
     2041 aaggccacgg acccgtcgac gacggcgggc acgactatgg cgtcggccag gtggccggcg
     2101 ggcacgctgg gccagaatcc ggtcaggcgc cagtcgccgt cgacctcgcg tgcttcaccg
     2161 cgacccgcgg gctcgacctc
//
LOCUS       DASTFV010000180         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_654302, whole genome shotgun sequence.
ACCESSION   DASTFV010000180 DASTFV010000000
VERSION     DASTFV010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654302"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VFP02_03390"
     CDS             <1..852
                     /locus_tag="VFP02_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9542098.1"
                     /translation="TGNRGWSPSSLTPFQVSYMQSGYRFRSPFRHLDDSDFIEACTED
                     LRDVIATQTAGDIAALIAEPVQGVGGFAIPPDGFFGPVEKVCREHDMLFIADEVQTGW
                     GRTGDHFWGHQAHGITPDLMTFAKGVGNGLTIAGVVGRAEVMDSVSANSISTFGGNPL
                     SCAGALANLHYLLDHGLQANTKARGAELMAALAPLSERHPAIGEIRGKGLMIGLEFVG
                     DDGLTPDAQVASAVLEGCKARGLLVGKGGLHGNCLRIAPPLSLTAEEAAEGAVILTTT
                     LDTVLGV"
     gene            complement(849..1631)
                     /locus_tag="VFP02_03395"
     CDS             complement(849..1631)
                     /locus_tag="VFP02_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9542099.1"
                     /translation="MPTTWEMVDEGWGRKASDFACLNEPAAAREYAFVHHRLALGDGD
                     RLLDAACGSGFALELARARGVTCAGIDAAPRLAAVARDRNPDSDIRVGDMEALPWDDA
                     SFDVVTSFRGIWGTTPGAVDEVHRVLVPGGRLALTVWGNVGKSPGAWMLAPFLWATDD
                     KVQHQADMVVLGRPGVGEAFLEDRGFEVAPRFEVPFAWEYPDPHTYARGVASTGPAYE
                     SIQSIGEEEFLARATALAEEHMRPGLPLRGQVQVFGYIGTKR"
     gene            1748..>2029
                     /locus_tag="VFP02_03400"
     CDS             1748..>2029
                     /locus_tag="VFP02_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542100.1"
                     /translation="MTETADAGPAERNGVPTEKLFGTIAKMKENGELAAFRFSARNEW
                     VEGTASRSSIYEWYGAGADQVHVEEFDFVADHPTLGHGHGPTPQEYVLHA"
BASE COUNT          323 a          746 c          684 g          276 t
ORIGIN      
        1 acggggaacc ggggctggtc gccgtcgtcg ctgaccccgt tccaggtcag ctacatgcag
       61 tccggctacc gcttccggag cccgttccgc cacctggacg acagcgactt catcgaggcc
      121 tgcaccgagg acctgcggga cgtgatcgcg acgcagaccg cgggcgacat cgccgcgctg
      181 atcgccgagc ccgtccaggg cgtcggcggc ttcgccatcc cccccgacgg cttcttcggc
      241 ccggtcgaga aggtgtgtcg ggagcacgac atgctcttca tcgccgacga ggtgcagacc
      301 ggctggggcc ggacgggcga ccacttctgg ggccaccagg cccacgggat cacccccgac
      361 ctgatgacgt tcgccaaggg ggtcggcaac gggctgacca tcgccggtgt ggtcggccgg
      421 gccgaggtca tggactcggt gtcggccaac tccatctcca ccttcggcgg caaccccctg
      481 tcctgcgccg gggcgctggc caacctccac tacctgctcg atcacggact ccaggccaac
      541 acaaaggccc ggggggccga actgatggcc gccctcgccc cgctgtccga gcgccacccc
      601 gccatcggcg agatccgggg gaagggcctg atgatcggcc tcgagttcgt gggtgacgac
      661 ggcctgaccc ccgacgccca ggtcgcctcg gccgtgctcg agggctgcaa ggcccgcggc
      721 ctgctggtcg gcaagggcgg cctccacggc aactgcctgc gcatcgcccc accactgtca
      781 ctgaccgccg aggaagccgc cgagggcgcg gtgatcctca ccacgaccct cgacaccgtc
      841 ctgggcgtct agcgcttggt cccgatgtag ccgaacacct ggacctgacc ccgcagcggc
      901 aagcccggcc gcatgtgctc ctcggcgagg gccgtggccc gggccaggaa ctcctcttcg
      961 ccgatgctct ggatggactc gtaggccggg ccggtggacg ccaccccccg ggcataggtg
     1021 tgcgggtcgg ggtactccca ggcgaagggc acctcgaagc ggggcgcgac ctcgaaccct
     1081 cgatcctcca ggaaggcctc gccgacgccg ggacgcccca acaccaccat gtccgcctgg
     1141 tgctggacct tgtcgtcggt ggcccagagg aacggggcga gcatccacgc gccgggggac
     1201 ttcccgacgt tcccccagac cgtcagggcc agtcgaccgc cgggaacgag cacacgatgg
     1261 acctcgtcca ccgcgccggg ggtggtaccc cagatcccac ggaagctcgt gacgacgtcg
     1321 aagctggcgt cgtcccacgg caacgcctcc atgtcaccga cccggatgtc gctgtcgggg
     1381 ttgcggtctc gagccaccgc ggcgaggcgg ggggccgcgt cgatgccggc gcaggtcacg
     1441 ccccgggctc gggccagctc caaggcgaac cccgacccac acgcggcgtc gagcagccgg
     1501 tcgccgtcac cgagggccag gcgatggtgc acgaaggcgt actcgcgagc ggccgcgggc
     1561 tcgttcaggc aggcgaagtc gctggctttg cgtccccagc cctcgtccac catctcccag
     1621 gtcgtgggca tgccgagtgg cctctcctcg atgtcggccg acagacaccg gagacgcgga
     1681 ccattcaccc cgaggcctcc ggtgagcttg gtaatcttcg cgcacgcgac agagagggaa
     1741 cgagaaggtg acggagaccg ccgacgcagg ccccgccgag cgcaacggtg tacccaccga
     1801 gaagctgttc ggcaccatcg ccaagatgaa ggagaacggc gagctcgccg ccttccggtt
     1861 ctcagcccgg aacgaatggg tggagggaac cgccagtcgg tcctcgatct acgagtggta
     1921 cggcgccggt gccgaccagg tccacgtgga ggagttcgac ttcgtcgccg accatccgac
     1981 gctcggccat gggcacggcc ccacgcccca ggagtacgtg ctccacgcc
//
LOCUS       DASTFV010000181         4824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_661978, whole genome shotgun sequence.
ACCESSION   DASTFV010000181 DASTFV010000000
VERSION     DASTFV010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661978"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFP02_03405"
     CDS             <1..224
                     /locus_tag="VFP02_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HET9542101.1"
                     /translation="YRRCCHPYGCDRTGGRLQTDHIVEHQHGGPTDTANGQPLCGTHN
                     RWKTNHQNDRPTEAPVDRDQRRTQPHPGP"
     gene            complement(370..909)
                     /locus_tag="VFP02_03410"
     CDS             complement(370..909)
                     /locus_tag="VFP02_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542102.1"
                     /translation="MPTRSPWLVLVPLVALLAGCSGDDGDAAGSDSTATSTLDPGVTF
                     EDDVTVPSGSGASTTVPPEGDVVSVPGQVPDRFPGRFPVPEGAEVEIGSSGHAEGELR
                     LSVDYTIADEDPAAVMDFYRDAVTQAGYSILLDEEAGTRRDYVGTLVFETDTYVGNVL
                     VSADGRRDVLLSLTATLPD"
     gene            complement(896..3733)
                     /gene="gcvP"
                     /locus_tag="VFP02_03415"
     CDS             complement(896..3733)
                     /gene="gcvP"
                     /locus_tag="VFP02_03415"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HET9542103.1"
                     /translation="MIPPFAHRHIGPDGAEIEAMLAAVGVASLDALVDAAVPASIRQD
                     TRLALPEAGTEEEILTRLRAYADRNQVAVSMLGMGYSGTLTPTVILRNVMENPAWYTA
                     YTPYQPEISQGRLEALLNFQTMITDLTGMDLANASLLDEATAAAEAMALCRRVTKRDS
                     ATFFVDAECHPQTIEVVATRAEPLGIKVIVGDPVTGLDGIDAYGVLVQYPGTSGGIRD
                     LTPIVEQVHEQGGLVVVAADLLALTLLEAPGAAGADVVVGSSQRFGVPLGYGGPHAAY
                     MSVREVHARSLPGRLVGVSVDEAGRPAHRLALQTREQHIRREKATSNICTAQVLLAVM
                     ASMYAVYHGPDGLTAIAEQVHERAGRLAAGLAGAGIGLEHEHFFDTVTAVVPGRAAEV
                     VAAAGERGINLRLLDADRVGIALDETTTPAHVEAVWAAFGVEGTYEEVTAESALPGDL
                     VRTSAFLTHPVFHQHRSETEFLRYVRSLADKDIALDRSMIPLGSCTMKLNATTEMIPV
                     TWPGFAALHPFAPLDQAEGYRELITELEAWLAEITGYDAVSVQPNAGSQGELAGLLAI
                     RDYHRQRGDAHRDVCLIPSSAHGTNAASAVMAGLRVVVVACDADGNVDLDDLQARVVE
                     HGDALAALMVTYPSTHGVFEEHIGEVCAAVHDAGGQVYLDGANLNAMVGIARPGKFGA
                     DVSHLNLHKTFCIPHGGGGPGVGPVAVREHLAPFLPGHPVVPEAGPLTGPGPVAAAPW
                     GSAGILPISWAYIALMGPDGLRRATEVAVLNANYVATRLRAHFPVLYTGEGGLVAHEC
                     ILDVRGLTKDTGVTAEDVAKRLIDYGFHAPTLSFPVPGTLMVEPTESESQAELDRFCD
                     AMIAIRTEIDRVASGEWPVEDSPLRHAPHTAEDVVADDWSRPYSREAAAYPVASLRRQ
                     KYWAPVGRIDQGYGDRNLQCECPPVSAYADA"
     gene            complement(3730..4773)
                     /gene="gcvT"
                     /locus_tag="VFP02_03420"
     CDS             complement(3730..4773)
                     /gene="gcvT"
                     /locus_tag="VFP02_03420"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET9542104.1"
                     /translation="MNLRLSPLDAAHRALGAKMVPFGGWDMPLSYGDGTIAEHRACRT
                     GAVAFDVSHLGTVRVTGPEAKGVLQANLSADLDKIEPGRTQYTHLLDADDASVLDDII
                     VWWLGEDRFDVMPNASNTAGVLAAIGGVDVTPTRAVIAVQGPRARDLFPAIADVGRRR
                     VVEMDLDGVAVTVAGTGYTGEDGVEVAVAAEHAVAVWDSVLARGVVPAGLGARDTLRL
                     EAGLPLHGHELGPGITPLQAGLGWVVGWDKGDFRGRAALEAERAAGPSRRLRGLLLDG
                     KRPPRADQSVVDGDAVVGAVTSGNYSPMLDRGIALAFVRPDIEPGARLAVDVRGRPEP
                     AEVVTPPFWTGAP"
BASE COUNT          686 a         1741 c         1774 g          623 t
ORIGIN      
        1 agtaccggcg ctgctgtcac ccctacggct gtgaccgcac cggcggtcgc ctccaaaccg
       61 accacatcgt cgaacaccaa cacggcgggc ccaccgacac cgccaacggc caacccctct
      121 gcggcaccca caaccgctgg aagaccaacc accagaacga ccggcccacc gaggcccccg
      181 tcgaccggga ccaacgaaga acccagcccc acccggggcc atgaccaacc aatcgacaca
      241 gccgaacacg ccccgcggca cccgtcacgc cccgatcaac ctcaccccga gccgccagcc
      301 cgcccccgac gacccacagc cctacgccgc atccccgcct cggtgtcagt cctccagagc
      361 gaaggcgagt cagtccggca gcgtcgcggt gaggctcagc aggacgtcgc gacgaccgtc
      421 ggcggagacc aggacgttgc cgacgtaggt gtcggtctcg aagaccagcg tgccgacgta
      481 gtcccggcgg gtgccggcct cttcgtcgag caggatcgag tagccggcct gggtgacggc
      541 gtcgcggtag aagtccatga cggcggccgg gtcctcgtcg gcgatcgtgt agtcgaccga
      601 caaccggagc tcaccctcgg cgtgaccgga cgagccgatc tcgacctcgg ccccttcggg
      661 aacggggaag cggcccggga accggtcggg cacctgaccg ggcaccgaca cgacgtcgcc
      721 ctcggggggc acggtggtgc tggcgccgga gcccgacggc acggtcacgt cgtcctcgaa
      781 ggtgaccccc gggtccaggg tcgaggtcgc cgtcgagtcc gacccggcgg cgtcaccgtc
      841 gtccccgctg cagccggcca gcaaggcgac caacgggacg aggaccagcc aggggctacg
      901 cgtcggcata ggccgagacg ggcgggcatt cgcactgcag gttgcggtcg ccgtagccct
      961 ggtcgatgcg accgaccggg gcccagtact tctggcgacg cagggacgcc accggatagg
     1021 ccgccgcctc cctgctgtag ggccgcgacc agtcgtcggc cacgacgtcc tccgccgtgt
     1081 ggggcgcgtg gcgcagaggg ctgtcctcga cgggccactc gcccgacgcc acccggtcga
     1141 tctcggtccg aatggcgatc atggcgtcac agaagcggtc caactcggcc tggctctcgg
     1201 actcggtggg ctcgaccatg agcgtgcccg gcacggggaa cgacagggtc ggggcgtgga
     1261 agccgtagtc gatcaggcgc ttggccacgt cctcggcggt cacgccggtg tccttggtca
     1321 ggccccgcac gtcgaggatg cactcatggg ccacgaggcc cccctcgccc gtgtagagca
     1381 ccgggaagtg ggcccggagg cgggtggcca cgtagttggc gttgagcacc gccacttcgg
     1441 tggcccggcg caggccgtcg gggcccatga gggcgatgta ggcccaggag atgggcagga
     1501 tcccggcgga gccccagggg gcggccgcca ccgggccggg gccggtcagc gggccggcct
     1561 cggggaccac cgggtgcccg ggcaggaacg gggccaggtg ctcccgcacg gccaccgggc
     1621 ccacaccggg gcctccccca ccgtgcggga tgcagaacgt cttgtgcagg ttgaggtggg
     1681 acacgtcggc cccgaacttg ccgggccggg cgatgccgac catcgcgttc aggttggccc
     1741 cgtccaggta cacctgcccg ccggcgtcgt ggacggcggc gcagacctcg ccgatgtgct
     1801 cctcgaagac accgtgggtc gaggggtagg tgaccatgag ggcggccaag gcgtcgccgt
     1861 gctcgaccac cctggcctgc aggtcgtcca ggtcgacgtt gccgtcggcg tcgcaggcca
     1921 cgaccacgac ccgcagtccg gccatgaccg ccgaggcagc gttggtgccg tgggccgagg
     1981 acgggatcag gcacacgtcc cggtgggcgt cgccccgctg gcggtggtag tcgcggatgg
     2041 cgaggaggcc ggccagctcg ccctgggacc cggcgttggg ctgcaccgac accgcgtcat
     2101 agccggttat ctccgccagc caggcctcga gctcggtgat cagctcccgg tagccctcgg
     2161 cctggtccaa cggggcgaag gggtgcaggg ccgcgaaccc cggccaggtc accgggatca
     2221 tctcggtggt ggcgttcaac ttcatggtgc aggagcccag ggggatcatc gaccggtcca
     2281 gcgcgatgtc cttgtcggcc agcgagcgca catagcgcag gaactcggtc tcggaccggt
     2341 gctggtggaa gaccgggtgg gtcaggaagg cgctggtccg gaccaggtcg cccggcaggg
     2401 ccgactcggc cgtcacctcc tcgtaggtcc cctccacgcc gaaggcggcc cagaccgcct
     2461 ccacgtgggc cggcgtcgtc gtctcgtcca aggcgatgcc gacccggtcg gcgtcgagca
     2521 ggcgcaggtt gatcccccgc tcccccgcgg cggccacgac ctccgccgcc cggccgggga
     2581 ccaccgcggt cacggtgtcg aagaagtgct cgtgctcgag gccgatgccc gccccggcca
     2641 ggccggcggc cagccgcccg gcccgctcgt ggacctgctc ggcgatggcc gtcaggccgt
     2701 cgggcccgtg gtacacggcg tacatcgagg ccatcacggc caacaggacc tgggccgtgc
     2761 agatgttgct ggtggccttc tccctgcgga tgtgctgctc ccgggtctgc agggccagcc
     2821 ggtgggcggg gcggccggcc tcgtcgaccg agacgccgac caggcgaccc ggcagggagc
     2881 gggcgtggac ctcccgcacg ctcatgtagg cggcgtgcgg gccgccgtag cccaggggca
     2941 cgccgaagcg ctggctggag ccgaccacga cgtcggctcc ggccgcgccg ggtgcctcga
     3001 gcagggtcag ggccagcagg tcggcggcca cgaccaccag gccgccctgc tcgtggacct
     3061 gctcgacgat cggggtcagg tcccggatgc cgccgctggt gcccgggtac tggacgagca
     3121 cgccgtaggc gtcgatgccg tcgaggccgg tcacggggtc gccgacgatg accttgatgc
     3181 ccaggggctc ggcccgggtg gccaccacct cgatcgtctg cggatggcac tcggcgtcca
     3241 cgaagaaggt ggccgagtcc cgcttggtca cccgacggca gagggccatg gcctcggctg
     3301 cggccgtggc ctcgtccagc aggctggcgt tggccaggtc catcccggtg aggtcggtga
     3361 tcatggtctg gaagttgagg agggcctcga gccggccctg ggagatctcc ggctggtacg
     3421 gcgtgtaggc cgtgtaccag gccgggttct ccatgacgtt gcgcaggatg accgtcgggg
     3481 tgagcgttcc cgagtagccc atgccgagca tggagacggc cacctggttg cggtcggcgt
     3541 aggcccggag ccgagtcagg atctcctctt cggtgcccgc ctcgggcagg gccaggcggg
     3601 tgtcctggcg gatgctggcg ggtacggcgg cgtcgaccag ggcgtccagg gaggccacac
     3661 cgaccgcggc cagcatggcc tcgatctcgg cgccgtcggg gccgatgtga cggtgggcga
     3721 agggcgggat cacggtgctc ctgtccagaa gggtggggtg acgacctcgg cgggctcggg
     3781 gcgaccccgc acgtcgacgg ccagccgggc gccgggctcg atgtcggggc ggacgaaggc
     3841 cagggcgatg cctcggtcca gcatgggcga gtagttgccg ctggtcacgg cgccgacgac
     3901 ggcgtcaccg tcgaccaccg actggtccgc ccggggcggg cgcttgccgt ccagcagcag
     3961 gccccgcagg cggcggctcg ggcccgcggc ccgctcggcc tccagcgccg cccgtccccg
     4021 gaagtcgccc ttgtcccagc ccacgaccca gcccaacccc gcctgcagcg gggtgatgcc
     4081 cgggcccagc tcgtggccgt ggaggggcag gcccgcctcc aggcgcagtg tgtcccgcgc
     4141 gcccaggccg gcggggacca ccccgcgggc caggacgctg tcccagacgg cgaccgcgtg
     4201 ctcggcggcc accgccacct cgaccccgtc ctcgcccgtg tagcccgtcc ccgcgacggt
     4261 gacggccacc ccgtcgaggt ccatctccac gacccgacgg cggccgacgt cggcgatcgc
     4321 cgggaacaga tcgcgggccc gaggcccctg cacggcgatg acggcccggg tgggcgtcac
     4381 gtcgaccccg ccgatggcgg ccagcacgcc tgccgtgttg gaggcgttgg gcatgacgtc
     4441 gaagcggtcc tcccccagcc accacacgat gatgtcgtcg aggaccgacg cgtcgtcggc
     4501 gtcgagcagg tgcgtgtact gggtccgccc gggctcgatc ttgtccaggt cggcggagag
     4561 gttggcctgg agcacgccct tggcctccgg gcccgtgacc cggacggtgc cgaggtggct
     4621 gacgtcgaag gcgaccgcgc cggtccggca ggcccggtgc tcggcgatcg tgccgtcgcc
     4681 gtaactcagg ggcatgtccc agccgccgaa gggcaccatc ttcgccccga gggcgcgatg
     4741 ggccgcgtcg agcggcgaga gccggagatt caccccgaga ttctaaggag cggcccggtg
     4801 cgatctacgc ggccgagtcc atgc
//
LOCUS       DASTFV010000182         2980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_669932, whole genome shotgun sequence.
ACCESSION   DASTFV010000182 DASTFV010000000
VERSION     DASTFV010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669932"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..851
                     /locus_tag="VFP02_03425"
     CDS             192..851
                     /locus_tag="VFP02_03425"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET9542105.1"
                     /translation="MDSKPDDPAAQLADELRGAIEELSSVAHDPERVAQAVRMAADLR
                     TFLDGPKAPRWYEASRVNGHLDEVSAAAFNNQSLYRGAHNPLAPPMAMALVEDDEGRR
                     IEARVRLNVAYEGPPHGVHGGYVAGMFDEVLGATQRMMAKPGVTGRLTVHYRHITPVE
                     EDLLLKAWITEDVGRRIVAKATCHAGDTLTAEADGLFIRVDFDEVADRMAARRDGGSV
                     T"
     gene            928..1440
                     /locus_tag="VFP02_03430"
     CDS             928..1440
                     /locus_tag="VFP02_03430"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9542106.1"
                     /translation="MDEGEGDTRRGLDQKAGLPDARRVVAALNPSLTRAAAAGLAVAI
                     VALDIDDMKVLNDRYGVPVGDRVLAAVASCLVDQARRFDIVARSEGDEFMVVLLDAGA
                     AVATAWAELARAALEELEVPPVASRIDAAFGVAVHDGVESGNALVQRANDAMVRARRA
                     GGNPVVVSVD"
     gene            complement(1472..2203)
                     /locus_tag="VFP02_03435"
     CDS             complement(1472..2203)
                     /locus_tag="VFP02_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET9542107.1"
                     /translation="MITSTDLPVPDRALAIAAHPDDVEFNCGGTFAKWSAAGCVVNHL
                     ILTDGSKGTWDPEQDLEALVALRQEEQRAAARALGSTGDVGFCGWVDGELDVTLERRG
                     VVAWWIRHVRPDVVLAHDPWKRYRIHPDHRAAGFLATDGIVAARDPHFFPEHDVDPWR
                     PTTLLLFEADDPDHVEDVTDWTDPKLAALECHESQFETTMFKDLGEVESAREAFRARE
                     RQKMVEMGEVASFRYGETFTLVSDI"
     gene            complement(2200..2784)
                     /gene="cofC"
                     /locus_tag="VFP02_03440"
     CDS             complement(2200..2784)
                     /gene="cofC"
                     /locus_tag="VFP02_03440"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HET9542108.1"
                     /translation="MLVLVPVKAFGRAKVRLAEVLDGPSRAALAREMADRVLTAAAPL
                     PVAVVCDDDDVAAWAESRGARVLWRPGHGLNGAVNDAVTTLAGEGLDRVMVTHADLPL
                     ATGFDELLAGDDGGAVLVPDRHEDGTNVAVVPAASGFTFAYGPGSFPRHCAEAERLGL
                     TLTVHRPPRLTWDVDVPADLDLPPDVPTTPLLAT"
     gene            2811..>2980
                     /locus_tag="VFP02_03445"
     CDS             2811..>2980
                     /locus_tag="VFP02_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542109.1"
                     /translation="MDVRVVVLGAGSWGTTVAHLAAHNTPTTLWARRADVAEEINTSH
                     TNSRYLADFTLH"
BASE COUNT          436 a         1035 c         1102 g          407 t
ORIGIN      
        1 ccagcgagaa gggtcggccc tgcttggcgg cggccacgcc ccgcagcacg gcggtctggt
       61 cgatgaggtt gagggtgccc cgctggtcgt cctcccccca ccgcccccag ttggagacac
      121 gggcggccag ttccttgagt tcgggcggga gtgccatggt cccccagcct cgcgcgtgca
      181 tgatgcgggc catggacagc aagcctgacg accccgccgc ccaactggcc gacgagctcc
      241 ggggcgccat cgaggagctg agctcggtgg cccacgaccc cgagcgggtg gcccaggcgg
      301 tgaggatggc ggccgacctg cgcacgttcc tcgacggtcc gaaggcaccc cgctggtacg
      361 aggccagccg ggtcaacggg cacctcgacg aggtcagcgc cgccgccttc aacaaccaga
      421 gcctctaccg gggtgcgcac aaccccctgg ccccgcccat ggccatggcg ctggtcgagg
      481 acgacgaggg ccgtcgcatc gaggcccggg tccgactcaa cgtcgcctac gagggcccgc
      541 cccacggtgt ccacggcggc tacgtggccg ggatgttcga cgaggtcctc ggcgccactc
      601 agcggatgat ggcgaagccg ggcgtgaccg gccgcctgac cgtccactac cgccacatca
      661 cccccgtcga ggaggacctg ctcctcaagg cctggatcac cgaagacgtc ggccgccgca
      721 tcgtcgccaa agccacctgc cacgccggcg acaccctgac cgcggaggcc gacggcctgt
      781 tcatccgggt cgacttcgac gaggtcgccg accgcatggc cgcccgccgt gacggtggtt
      841 ctgttacgtg atcttcgcac gaatgtcacg aaaccgacac gaagggcgcg gtgacggggc
      901 aggatgaccg aacgcaggag caacccgatg gatgagggtg agggtgacac ccgccgtggc
      961 ctcgatcaga aggccggcct tccggacgcc cgacgcgtcg tcgccgctct gaacccgtca
     1021 ttgacccgag cggcagccgc cgggctcgcc gtcgccatcg tggccctcga catcgacgac
     1081 atgaaggtgt tgaacgatcg ctacggcgta ccggtcggcg accgcgtgct ggccgcggtg
     1141 gcatcgtgcc tcgtcgacca ggcccgccgc ttcgacatcg tcgctcgctc cgagggcgac
     1201 gagttcatgg tcgtcctgct ggacgccggg gctgccgtcg ccaccgcttg ggccgaactg
     1261 gctcgcgccg cgctcgagga gctggaggta cctccggtgg cctcccggat cgacgccgcc
     1321 ttcggtgtcg ccgtccatga cggcgtcgag agcgggaatg ccctcgtgca acgggccaac
     1381 gacgcgatgg tccgggcccg acgggccggc gggaacccgg tcgtggtgag cgtggactga
     1441 tcaggcgtcg accgccgtcg ccgcccgtgg atcagatgtc ggagaccagc gtgaacgtct
     1501 cgccgtagcg gaagctggca acctcgccca tctcgaccat cttctgtcgc tccctggcgc
     1561 ggaaggcctc gcgtgcggac tcgacctcgc cgaggtcctt gaacatggtg gtctcgaact
     1621 gggactcgtg gcactcgagg gcggcgagct tggggtcggt ccagtcggtg acgtcttcga
     1681 cgtggtcggg gtcgtcggct tcgaacagga ggagcgtggt cgggcgccag gggtcgacgt
     1741 cgtgttcggg gaagaagtgg gggtccctcg cggcgacgat gccgtcggtg gccaggaagc
     1801 cggcggcgcg gtggtcgggg tggatgcggt agcgcttcca ggggtcgtgg gcgaggacga
     1861 cgtcggggcg gacgtggcgg atccaccagg cgacgacgcc tcggcgctcc agggtgacgt
     1921 ccagctcgcc gtcgacccag ccgcagaagc cgacgtcgcc ggtcgagccc aaggcccggg
     1981 ctgccgcccg ctgctcctcc tggcggaggg cgacgagcgc ctcgaggtcc tgctcgggat
     2041 cccaggtgcc cttcgacccg tcggtgagga tcaggtggtt caccacgcaa cccgccgccg
     2101 accacttggc gaaggtgccg ccgcagttga actcgacgtc gtcggggtgg gcggcgatgg
     2161 ccagggcccg gtcggggaca gggaggtcgg tcgaggtgat cacgtcgcca ggagcggggt
     2221 ggtgggcacg tccgggggca ggtcgaggtc agccgggacg tccacgtccc aggtcagccg
     2281 gggtgggcgg tgcacggtca gggtcaaccc gagccgctcg gcttcggcgc agtggcgggg
     2341 aaacgagccc ggcccgtagg cgaaggtgaa gccggaggcg gccgggacga cggccacgtt
     2401 ggtcccgtcc tcgtggcggt cggggaccag gacggcgcca ccgtcgtctc ccgcgagcag
     2461 ctcgtcgaag cccgtggcca gcggcaggtc ggcgtgggtg accatgaccc ggtcgaggcc
     2521 ctcgccggcc agcgtggtga cggcgtcgtt gaccgcgccg ttcaggccgt ggcccggtcg
     2581 ccacaggacg cgggcgccgc gggactcggc ccaggccgcg acgtcgtcgt cgtcgcagac
     2641 cacggcgacg ggcagggggg cggcggcggt cagcacgcga tcggccatct cccgggccag
     2701 cgctgctcgc gacgggccgt ccagcacctc ggccaaccgg accttggccc gcccgaaggc
     2761 cttgaccggc acgaggacga gcatcgaagg gcgagtctag ggtggccctc atggacgtcc
     2821 gagtggtggt tctcggcgcc gggtcgtggg ggacgacggt cgcccacctc gccgcgcaca
     2881 acacgccgac cacgctctgg gcccgccgcg ccgacgtggc cgaggagatc aacaccagcc
     2941 acaccaacag ccgctacctg gcggacttca ccctccacga
//
LOCUS       DASTFV010000183         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_674512, whole genome shotgun sequence.
ACCESSION   DASTFV010000183 DASTFV010000000
VERSION     DASTFV010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674512"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VFP02_03450"
     CDS             <1..891
                     /locus_tag="VFP02_03450"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HET9542110.1"
                     /translation="IVVGSTVIDRAIVEGQRNRVDEVRDNLDERQAANDALQAQVDDL
                     EAFANDAAAITVDGRLTDTVAVVVTDEGVDRDPVDRTIELLGQAGSTVRGVLTVDSSW
                     VLEDPEQMAALAEVVDLDAESTVEEIQTRAASLLVTDLSSSVEVVDDGTGALDEISAL
                     DLVDYEALTDVVAPRPRRVLFVVVSGPASTLESSAHTESFAVAAAASAGAVVVAEVWR
                     EEENGPDRGDSLATILGSPTLRGQISTVDDLDLAQGPTTAILTLLAAQAGEIGHYGVG
                     DGADAAAPPPPGPPTTTTVP"
     gene            888..>2149
                     /locus_tag="VFP02_03455"
     CDS             888..>2149
                     /locus_tag="VFP02_03455"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HET9542111.1"
                     /translation="MTAPNSVGRSAVGMGAAAAASRVLGGARVLVVAAVLGTTWLGDT
                     FEAANTFPTVVFELLAAGALSAVLVPTFVDLFARDQRPEAERQAGGLLGLALVVLGSL
                     AVVGMIAAPWLADALTRASDAPAQQQELATSLLRWFLPQLLLYPIGFCAIALLNAKRV
                     FALPAAAPIGNTVVLVSALLIFYAVAGPDPGLDLTTGETALLGLGGTLGVAAFVAIPT
                     VALWRTGFRLWPRWAPRDPGVRRLLGLSSWAVLQHSGAALLLGAAIVLGAGVEGGVVS
                     YRVAFYLFLAPYGILAQPLHTTVQPELADDVDRGDLEGFGRAIRWALASLGVVVLPVA
                     GLMVALARPAMEVAAFGQADSGDGVALMAAGVAGLALGLPAYGAFRLLAAAWYALDDS
                     RTPALAAVASALIGVGLMIALVPVTDGAA"
BASE COUNT          239 a          825 c          756 g          329 t
ORIGIN      
        1 atcgtcgtcg ggtcgacggt catcgaccgc gccatcgtcg agggccaacg caatcgggtc
       61 gacgaggtcc gcgacaactt ggacgagcgc caggcggcca acgacgccct ccaggcccag
      121 gtcgacgacc tcgaggcgtt cgccaacgac gccgcggcca tcaccgtcga cggccggctg
      181 accgacaccg tggccgtcgt cgtcaccgac gaaggcgtgg atcgggatcc ggtcgaccgg
      241 acgatcgaac tgctcggcca ggccggctcg accgtccggg gcgtgctcac ggtcgacagc
      301 tcgtgggtcc tggaggaccc cgagcagatg gcggcgctgg ccgaggtcgt cgacctcgac
      361 gccgagtcca ccgtcgagga gatccagacc cgggcggcct ccctcctcgt caccgacctg
      421 tcgtcgtcgg tcgaggtggt cgacgacggc accggtgccc tcgacgagat cagcgctctg
      481 gacctggtcg actacgaggc cttgaccgac gtggtcgccc cccggccccg gcgggtcctc
      541 ttcgtggtcg tgtccggccc agccagcacc ctcgagagct cggcccacac cgagtcgttc
      601 gccgtcgccg cggccgcgtc ggcgggcgcg gtggtcgtgg ccgaggtgtg gcgggaggaa
      661 gagaacggcc cggaccgagg tgactcactg gccaccatcc tcggctcacc gacgctgcgg
      721 ggacagatct cgacggtgga cgacctggac ctggcccagg gtcccaccac cgcaatcctc
      781 accctcctgg ccgcccaggc gggcgagatc gggcactacg gcgtgggcga cggtgccgat
      841 gcggccgccc caccgccgcc cgggccgccg accacgacca cggtcccgtg accgccccga
      901 actccgtcgg ccggtccgcg gtcgggatgg gggcggcggc ggccgcctcc cgggtcctcg
      961 gcggggcccg cgtcctcgtc gtcgccgccg tcctgggcac gacctggctg ggggacacct
     1021 tcgaggcggc gaacaccttc ccgacggtcg tcttcgagct gctggcggcg ggggccctgt
     1081 ccgccgtgct cgtcccgacc ttcgtcgatc tcttcgcccg cgaccagcga cccgaggccg
     1141 aacgccaggc cggtggcctg ctcgggctgg ctctggtggt cttgggctca ctcgccgtcg
     1201 tggggatgat cgccgccccc tggctggccg acgcgttgac cagggcctcc gacgccccgg
     1261 cccaacagca ggagctggcg acttccctgc tgcgctggtt cctgccccaa ctgctgctct
     1321 atccgatcgg cttctgcgcg atcgccctcc tcaacgccaa gcgggtgttc gccctgccgg
     1381 cggcggcccc gatcggcaac acggtcgtcc tggtgtccgc ccttctgatc ttctacgccg
     1441 tggccggacc cgatcccggg ctcgacctga cgacgggcga gacggcgctg ctcggcctcg
     1501 gcgggacctt gggtgtggcc gccttcgtgg ccatcccgac ggtggccctg tggcgcaccg
     1561 gattccggtt gtggccgcgg tgggctccac gtgaccctgg cgtccgtcgc ctcctcgggc
     1621 tctccagctg ggccgtgctc cagcacagcg gcgccgccct cctgctgggc gccgccatcg
     1681 tcctcggggc cggcgtcgag ggcggggtgg tctcgtaccg ggtcgccttc tacctgttcc
     1741 tggcccccta cgggatcctg gcccaaccgc tgcacaccac cgtccagccc gagttggccg
     1801 acgacgtgga tcggggcgac ctcgaaggct tcggccgggc catacgctgg gcgctggcgt
     1861 cgctcggcgt cgtggtcctc ccggtggccg ggctcatggt ggccctggcc cgcccggcca
     1921 tggaggtggc cgccttcggt caagccgact cgggcgacgg cgtggccctg atggccgcgg
     1981 gcgtggccgg cctggccctc ggcctgccgg cctacggcgc cttccgcctg ctggccgcgg
     2041 cctggtacgc cctcgacgac agccgcacgc cggccctcgc ggcggtcgcc tccgccctga
     2101 tcggcgtggg cctcatgatc gccctcgtcc cggtgaccga tggtgcggc
//
LOCUS       DASTFV010000184         3068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_682397, whole genome shotgun sequence.
ACCESSION   DASTFV010000184 DASTFV010000000
VERSION     DASTFV010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682397"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VFP02_03460"
     CDS             complement(<1..305)
                     /locus_tag="VFP02_03460"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9542112.1"
                     /translation="MTLPATMPAAVYREKGVLEVVDVDTPGLGPHDVLLEVSHCGVCG
                     SDLHMVLEGWGTPDAIEGHEYTGTVAAVGAEVTRWTVGQPVVAGPSRRCGECGPCLAG
                     "
     gene            complement(302..784)
                     /locus_tag="VFP02_03465"
     CDS             complement(302..784)
                     /locus_tag="VFP02_03465"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9542113.1"
                     /translation="MREHRYTLDMPHSAARLWALFQDYDSWTKYAPMVTAVEVVHPGD
                     QHGNGLLRRVIYQLPMGRKGAALELVTEVAKERGYTFTMLSHKPGNDQTGKVRLEPLG
                     PNQTRFHFEERYHLTSAPWKWFEGRIYGFINKKNEETMRAAAQWLTDHPEYRPDLVSR
                     "
     gene            complement(784..2049)
                     /locus_tag="VFP02_03470"
     CDS             complement(784..2049)
                     /locus_tag="VFP02_03470"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9542114.1"
                     /translation="MGDRTWTTGMAKSEAHGLLPDPEPRDIRYTVISVDDHLVEPPGM
                     FEGRLPARFQDAAPRVVETEEGHEVWEFDGQIFLQVGLNAVVGRPREDWKIEPTRFED
                     MRPGCWDIDARVKDMDINGVWAALNFPSQITGFCGRVFSQCSDPALGLEVTKAWNDWF
                     HDEWWQPHPERNIPMGITYLADAEAGAAEIRRNAERGFRAVTLPEQPHRIGLPPIFSD
                     HWDPIIEACADTGTVVCLHVASCGIVDLPKGAPAIQLGATLFGQLALGACAEWLWSGY
                     PVRFPDLQIAMSEGGIGWVAMLIDRLDNMLERSGYGRDFTGSFGMPSDLRPSDVLRRN
                     FTFCTIDDPGTISTRDVIGVENICFETDYPHGDGTWPDTQAVIEKAYGGILTDAEIAA
                     ITHENAARVFRHPLPPADSPHAAGLRNPT"
     gene            2115..>3068
                     /locus_tag="VFP02_03475"
     CDS             2115..>3068
                     /locus_tag="VFP02_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HET9542115.1"
                     /translation="MPLPRVRAPDTEEFRAGFAAVRERLDIPATMPAEVEAAGAEAAR
                     RAVTADRVDRRDLPLVTIDPVGSKDLDQALHIEARGDGHRVHYAIADVAAFVDAGDPV
                     DREAWHRIVTMYSPDHRAPLHPAALSEGAASLLPDGDRPAILWSIDLDGQGEVTERRV
                     ERALVRSRAQLGYEEAEALCDRGGHPALGELRTVGERRIARQAERGGISLHLPNQEVV
                     AGDHGYDVVFEAPLAIEHWNEQISLLTGMVAAELMLSAGTGLLRTLPPPPEFVVRALG
                     RSARGLGVDWPKRTHDYASILGGLDPARPEHAALITQAARLF"
BASE COUNT          416 a         1067 c         1132 g          453 t
ORIGIN      
        1 ccggcgaggc agggcccgca ctcaccgcag cggcgggagg gtccggccac gaccggctgg
       61 cccaccgtcc accgggtgac ctcggcgccg acggcggcga cggtgccggt gtactcgtga
      121 ccctcgatgg cgtcgggcgt gccccagccc tccagcacca tgtgcaggtc gctgccgcac
      181 acgccgcaat gggagacctc gagcaggacg tcgtggggcc cgaggcccgg ggtgtcgacg
      241 tcgaccacct ccaggactcc cttctcgcgg tagacggcgg cgggcatggt ggcggggagg
      301 gtcatcgcga caccaggtcg gggcggtact cgggatggtc ggtcagccac tgcgccgccg
      361 cccgcatggt ctcctcgttc ttcttgttga tgaagccgta gatccgcccc tcgaaccact
      421 tccacggcgc gctcgtcagg tggtaccgct cctcgaagtg gaaccgggtc tggttcgggc
      481 ccaggggctc gaggcggacc ttgcccgtct ggtcgttgcc cggcttgtgg ctcagcatgg
      541 tgaaggtgta gccccgctcc ttcgccacct cggtcaccag ctccagcgcc gcgcccttgc
      601 ggcccatcgg cagctggtag atcacccgcc gcagcaagcc gttcccgtgt tggtcaccgg
      661 gatgcaccac ctcgactgcg gtgaccatcg gggcgtactt cgtccagctg tcgtagtcct
      721 ggaacagggc ccacagccgg gcggcggagt ggggcatgtc gagggtgtag cggtgctcgc
      781 gcatcaggtc gggttccgga ggccggccgc gtggggactg tcggccgggg gcagcgggtg
      841 gcggaacacc cgggccgcgt tctcgtgggt gatggcggcg atctcggcgt cggtgaggat
      901 cccgccgtag gccttctcga tgacggcctg ggtgtcgggc caggtgccgt ccccgtgcgg
      961 gtagtcggtc tcgaagcaga tgttctcgac gccgatgacg tcccgggtgc tgatcgtgcc
     1021 cgggtcgtcg atggtgcaga aggtgaagtt gcggcggagc acgtcactgg gccggagatc
     1081 gctgggcatg ccgaacgagc cggtgaagtc ccggccgtag cccgagcgct cgagcatgtt
     1141 gtccagccgg tcgatgagca tggccaccca gccgatgccc ccttcggaca tggcgatctg
     1201 caggtcgggg aagcggacgg gatagcccga ccacagccac tcggcgcagg cgccgagggc
     1261 caactggccg aacagggtcg cccccagctg gatggcgggg gcccccttcg gcaggtcgac
     1321 gatcccgcag gacgccacgt ggaggcagac cacggtgccg gtgtcggcgc aggcctcgat
     1381 gatcgggtcc cagtggtccg agaagatcgg gggcaggccg atgcggtggg gctgctcggg
     1441 cagggtcacg gcccggaagc cccgctcggc gttgcgccgg atctctgccg ccccggcctc
     1501 ggcgtcggcc aggtaggtga tgcccatggg gatgttgcgt tcgggatggg gctgccacca
     1561 ctcgtcgtgg aaccagtcgt tccacgcctt ggtcacctcg aggccgagcg cggggtccga
     1621 gcactggctg aacacccgcc cacagaagcc ggtgatctgg gacgggaagt tcagcgcagc
     1681 ccagaccccg ttgatgtcca tgtccttcac gcgggcgtcg atgtcccagc agcccgggcg
     1741 catgtcctcg aagcgggtgg gctcgatctt ccagtcctcc cggggccggc ccacgacggc
     1801 gttgagcccg acctgcagga agatctgacc gtcgaactcc cagacctcgt gcccctcctc
     1861 ggtctccacg acccgggggg cggcatcctg gaaccgggcc gggaggcgac cctcgaacat
     1921 gccgggcggc tcgaccaggt ggtcgtccac cgagatcacc gtgtagcgga tgtcccgggg
     1981 ctcggggtcg ggcagcaggc cgtgggcctc gctcttggcc atgcccgtgg tccaggtccg
     2041 gtcccccatc gtcccggaga ctacgagatg cctgacacgg gcgtcagcgg gctcggtggc
     2101 tacggtcgct ctccgtgccg ctgcctcggg tgcgagcccc cgacaccgag gagttccggg
     2161 cgggcttcgc cgccgtccgg gagcgactcg acatcccggc cacgatgccg gccgaggtcg
     2221 aggcggcggg ggccgaggcg gcccgccggg ccgtgaccgc ggaccgggtc gaccgtcggg
     2281 atctcccgct cgtcaccatc gaccccgtcg gcagcaagga cctggaccag gccctgcaca
     2341 tcgaggcccg aggcgacggt caccgggtgc actacgccat cgccgacgtc gccgcgttcg
     2401 tggatgctgg tgacccggtc gaccgcgagg cgtggcatcg gatcgtcacg atgtacagcc
     2461 ccgaccatcg ggcaccgctg cacccggctg cgctgagtga gggggcggcc agcctcctgc
     2521 ccgacggtga ccggccggcc atcctgtgga gcatcgacct cgacgggcag ggggaggtca
     2581 ccgagcggcg ggtcgagcgg gccctggtcc gcagccgggc ccagctcggc tatgaggagg
     2641 ccgaggccct gtgtgaccgg ggcggccacc cggccctcgg cgagctgcgg acggtcggcg
     2701 agcgccggat cgcccgccag gccgagcggg gtggcatcag cctccacctg cccaaccagg
     2761 aggtcgtggc cggtgaccac ggctacgacg tggtcttcga ggccccgttg gccatcgaac
     2821 actggaacga gcagatctcg ctgctgaccg gcatggtcgc ggccgagctc atgctgagcg
     2881 ccgggaccgg gctgctgcgg acgctgccac cgccgccgga gttcgtcgtc cgcgccctcg
     2941 ggcgcagtgc ccggggcctc ggcgtcgact ggccgaagcg cacccacgac tacgcgtcga
     3001 tcctgggcgg gctggacccg gcccggccgg agcacgcggc cctgatcacg caggcggccc
     3061 gcctcttc
//
LOCUS       DASTFV010000185         3381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_688579, whole genome shotgun sequence.
ACCESSION   DASTFV010000185 DASTFV010000000
VERSION     DASTFV010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688579"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFP02_03480"
     CDS             <1..297
                     /locus_tag="VFP02_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET9542116.1"
                     /translation="TLDEWKEKLDTMDGVWAPMQSALELHDDPQAIANGYLPRLTTPE
                     GVDFALVASPVQFDQVSPELRTAPEHGQHTEEILLGRGMDWEQLAALKDDGSIL"
     gene            complement(320..652)
                     /locus_tag="VFP02_03485"
     CDS             complement(320..652)
                     /locus_tag="VFP02_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542117.1"
                     /translation="MSEQSAPAGRREQFEEEIAAVRIRTGKAEAEQRFTILGLIGLIG
                     GIGICIAAYIASGGQSDTRDVISSVILALVGVTVSILGAALFLRYSLARFLRFWLLRL
                     IYEQQDQK"
     gene            complement(682..2178)
                     /locus_tag="VFP02_03490"
     CDS             complement(682..2178)
                     /locus_tag="VFP02_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9542118.1"
                     /translation="MALLEARGVTVRFGGNVATDAVDLDVDAGGITGLIGPNGAGKTT
                     MFNALCGLQPMARGSVVLDGEDISKLAAHQRARRGLARTFQRLEVFTLLSVRENILSG
                     AEIRRTWARRAGPGARTDDRTPEEMADELIERLGLSDVAEDRVDSLPTGRARLVELGR
                     ALANLPRLILLDEVSSGLNETETEAMAAVLQGLAENGIGILLVEHDMSLVMSTCHDIH
                     VLDFGEIIAVGTPEETQTNPRVQEAYLGSGTTTHAKAVRRDLTSEEVPLLDVRGISAG
                     YGEFDVLDEVSFSVRTGEVFALLGPNGAGKSTTLKVVSGLLAPTSGSVAICGRSVTGA
                     DADALSRAGVCMVPEGKGIFPNLTVGENLEMATFTGTSKADIEERSYGRFPRLAERRS
                     QVAGTLSGGEQQMLSMARALATEPSLLLLDELSMGLAPIIVQGLYEQVAEIAREGLTI
                     VVVEQFAHEVLGVADTAAIMLNGRVRRVGPPEQIAEELTEAYLAGSTG"
     gene            complement(2159..>3381)
                     /locus_tag="VFP02_03495"
     CDS             complement(2159..>3381)
                     /locus_tag="VFP02_03495"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9542119.1"
                     /translation="DFTPISLSGLRPAFPVILLFIILLSLPPLRVRASGQQKSREYIP
                     MPPMRRWVAATVVLGALAWVAASQLSGIRTYQLGIGMGVGIIMLSLVPLIGWGGQINL
                     APLTFAGIGAVTMGHFGGSGTLWGLVLAAVVSGLVGALVALPALRLQGLYLALATAAF
                     AVFMEKMFFNQSIVLPNASLNVPRLEIGGLSFQGDKAHLVLLSVSFGLVALFLVFLRR
                     GEFARRLLAMKASPAACVTLGLNLTRTKLQVFTLSAAIAGFGGALLGAQTNSVSPDSF
                     RFLQGLPIVLLAVIGGISAVGGALFGGLTFAVAFFIIPDVAPSLTNIFALSPALAGVS
                     LGRNPNGAVNETVRSVKEKLASRRARAEGDVTEVEPDWDRLGLDEPLTVAERDRIDRE
                     LALDEEVLYGAARS"
BASE COUNT          544 a         1153 c         1209 g          475 t
ORIGIN      
        1 accctggacg agtggaagga gaagctggac acgatggacg gggtctgggc cccgatgcaa
       61 tccgccctcg agcttcacga cgacccgcag gccatcgcca acggctatct cccgaggctg
      121 accacgccgg aaggcgtcga cttcgccctg gtggcctcgc cggtccagtt cgaccaggtg
      181 tcacccgagc tgcgcacggc gcccgagcac ggccagcaca ccgaggagat cctcctcggc
      241 cggggcatgg actgggagca gctggccgcc ctcaaggacg acggctcgat cctgtaggcg
      301 gttctggaac cagaacgggt catttctggt cctgctgctc gtagatgagg cggaggagcc
      361 agaagcgcag gaagcgggcc agcgagtagc gcaggaacag cgccgcgccc aggatcgaca
      421 ccgtgacccc gacgagcgcc aggatcacgc tggagatgac gtcacgggtg tcgctctggc
      481 cgccggaggc gatgtaggcg gcgatgcaga tgccgatgcc gccgatgagg ccgatgaggc
      541 ccaggatcgt gaagcgttgc tccgcctccg ccttgcccgt gcggatgcgg accgcggcga
      601 tctcctcctc gaactgctcg cgccgccccg ccggtgccga ctgctcgctc atgggctctg
      661 ctctccttgg gtcacgaatg gtcagccggt cgagccggcc agataggcct cggtcaactc
      721 ttctgcgatc tgctcgggcg gcccgacgcg gcgcacccgt ccgttcaaca tgatggccgc
      781 ggtgtcggcc acgccgagga cctcgtgggc gaactgctcc acgaccacga tggtcagccc
      841 ctcgcgggcg atctccgcca cttgttcgta gaggccctgg acgatgatcg gggccaggcc
      901 catcgacagc tcgtcgagca ggagcaggct gggttcggtg gccagggccc gggccatgga
      961 cagcatctgc tgctcgcccc ccgacagcgt cccggccacc tggctgcggc gctcggccag
     1021 ccgcgggaag cggccgtagg accgttcctc gatgtcggcc ttgctcgtgc cggtgaacgt
     1081 ggccatctcc aggttctcgc ccacggtcag gttcgggaag atgcccttgc cctcggggac
     1141 catgcacacg cccgctcggg acagggcgtc ggcgtcggcc ccggtgaccg agcgaccgca
     1201 gatggccacg ctgcccgagg tcggggccag caggccgctg acgaccttga gcgtggtgga
     1261 cttgcccgcc ccgttgggcc cgaggagggc gaagacctcg ccggtgcgga ccgagaagct
     1321 gacctcgtcg agaacgtcga actcgccgta gccggcactg atcccgcgga cgtcgagcaa
     1381 cgggacctcc tcggaggtca gatcccggcg gacggccttg gcgtgagtgg tcgtaccgct
     1441 gcccaggtag gcctcctgca cccgcgggtt ggtctgggtc tcctcgggtg tgcccaccgc
     1501 gatgatctcg ccgaagtcga gcacgtggat gtcgtggcag gtgctcatga ccaggctcat
     1561 gtcgtgctcg acgagcagga tgccgatgcc gttctcggcc aggccctgga gcaccgcagc
     1621 catggcctcg gtctcggtct cgttcagccc cgacgagacc tcgtcgagca ggatcaggcg
     1681 agggaggttg gccagggccc ggcccagctc gaccagtcgg gcccggcccg tgggcaggct
     1741 gtcgacccgg tcctcggcca cgtcgctcag cccgagccgc tcgatgagct catcggccat
     1801 ctcctccggc gtgcggtcgt cggtccgcgc ccccggcccg gcgcgacggg cccaggtccg
     1861 acggatctcg gctccggaca ggatgttctc ccgcaccgag agcagggtga agacctcgag
     1921 acgctggaag gtgcgggcca gcccccggcg ggctcgctgg tgggccgcca gcttggagat
     1981 gtcctcaccg tcgagcacca ccgagccccg ggccatcggc tgcagcccgc acagggcgtt
     2041 gaacatcgtg gtcttgcccg ccccgttggg gccgatcagg ccggtgatgc cgccggcgtc
     2101 gacgtccagg tcgacggcat cggtggccac gttcccgccg aagcggacgg tgacccccct
     2161 agcttcgagc agcgccatag agcacctcct cgtccagggc cagctcccgg tcgatgcgat
     2221 cccgttcggc gacggtgagc ggttcgtcca ggccgaggcg gtcccagtcc ggttcgacct
     2281 cggtgacgtc gccctccgcc cgagctctgc gcgaggccag cttctccttg acgctgcgca
     2341 cggtctcgtt gaccgcaccg ttggggttac gaccgaggct gaccccggcc agggccgggc
     2401 tcagggcgaa gatgttggtc agcgacggag cgacgtcggg gatgatgaag aaggccacgg
     2461 cgaaggtcag gccgccgaac agggcgccgc ccacggcgct gatgccgccg atgaccgcca
     2521 ggagcacgat cgggaggccc tgcaggaagc ggaagctgtc cggggacacc gagttggtct
     2581 gcgccccgag cagcgcacca ccgaagccgg cgatggccgc cgacagggtg aagacctgca
     2641 gcttggtccg ggtcaggttg aggcccaggg tgacgcacgc cgccggactg gccttcatgg
     2701 ccagcagacg gcgggcgaac tcgccccggc gcaggaacac caggaagagg gccaccaggc
     2761 cgaaggagac cgacagcagc acgaggtggg ccttgtcgcc ctggaaggag agcccaccga
     2821 tctccagtcg cggcacgttc aggctggcgt tgggcagcac gatgctctgg ttgaagaaca
     2881 tcttctccat gaacaccgcg aacgcggcgg tggccagagc caggtagagg ccttggagtc
     2941 ggagggcggg caacgcgacg agcgccccga ccaggccgct gacgaccgcg gccagcacga
     3001 ggccccaaag ggtcccgctc cccccgaagt gacccatcgt caccgccccg atgccggcga
     3061 aggtcagggg ggccaggttg atctggccac cccaaccgat gaggggcacc agggacagca
     3121 tgatgatccc gacgcccatg ccgatgccga gctggtaggt ccggatgccg ctgagctggc
     3181 tggccgccac ccatgcgagg gcgccgagca cgacggtggc ggcgacccag cgccgcatcg
     3241 gcggcatggg gatgtactcc cgggacttct gctggccgga ggcgcggacc ctgagcggcg
     3301 ggagggagag gaggatgatg aagaggagga tcaccgggaa ggccgggcgg agcccgctga
     3361 gggagatcgg cgtgaagtcg t
//
LOCUS       DASTFV010000186         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_693406, whole genome shotgun sequence.
ACCESSION   DASTFV010000186 DASTFV010000000
VERSION     DASTFV010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693406"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFP02_03500"
     CDS             complement(<1..353)
                     /locus_tag="VFP02_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542120.1"
                     /translation="MPAEPTDPADPVGLNRPVAPLRPTDRLLALLADLGLSPRATLLG
                     FAVCLVALLGWLWLRSPAPPAAEAGLPLVPAATTALPGASSLPIVANGDTVLAHAAGA
                     VRRPGLYELAAGERVA"
     gene            complement(439..1146)
                     /locus_tag="VFP02_03505"
     CDS             complement(439..1146)
                     /locus_tag="VFP02_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9542121.1"
                     /translation="MTDVTDATFQTAVLDRSDVVPVVVDLWAPWCGPCTQLGPILEKV
                     IGATDGKVELVKVDIDENPQVSQAFQVQSIPAVYALKDGAVVDSFIGAQGEDAVQAFV
                     DGLLPSETENTIEALLAAGDEASLDQVLALVPDDERAVVALADLWVDGGRADEALELL
                     TRIPESPETRRVAAKARTGEAVVDGDEISGRLDGLLDRVKADDDARQEFVDLLELLGP
                     DDPRTAAYRKSLSQRLF"
     gene            1206..2264
                     /locus_tag="VFP02_03510"
     CDS             1206..2264
                     /locus_tag="VFP02_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET9542122.1"
                     /translation="MANTDEAETIPVEGIHPEEVTDWFATHVPEAQPPLAFDLLAGGR
                     SNLTYSVTDRTGARWVLRRPPLGHVLATAHDMGREHRIISALAPTPVPVAPVIGLSPD
                     DSVNGAPFYVMAFVDGPIVRNAEGARQLPVEHRARATESIVDVLADIHAVDVEAVGLG
                     DLGRHEGYIERQLKRWQGQWEKSKTRELPVIDAVHDRLALSVPGQVGVAIVHGDYRLD
                     NCVLDDEGTIQAVLDWELCTLGDPLADVGQLCMYWTEPGDTLVAAPDAPTTVEGFGTR
                     ADLLARYASTSGRDLAEIDYYIAFGFWKLACICEGVLKRYQAGVMGGPDASALVEMFT
                     HHVANLANAAATTLGMDP"
     gene            2261..>3096
                     /locus_tag="VFP02_03515"
     CDS             2261..>3096
                     /locus_tag="VFP02_03515"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HET9542123.1"
                     /translation="MSHPLYEFHQDPGLSEPVLLVALEGWIDAGGGAASAAETILASA
                     EQIPVATFNSDTLLDHRARRPIMHLVDGVNTGLTWPTIRLDETWDREGRDVLFLHGPE
                     PDHNWPSFAKAVIELAEGFEVSEVYGLGAYPAPVPHTRPVQLASTASSDDMAQRVGFI
                     RGTLEVPAGVAAAIEAQAAEIGMSALGLWAQVPHYAAAMPYPAAAVRLIDGLFEVAGL
                     RFDHGELDAEVETIRARLDELVANSDENQELVHQLEIHVDQSEPDPDDGGIELRSGDE
                     LAA"
BASE COUNT          460 a         1126 c         1086 g          424 t
ORIGIN      
        1 gccacccgtt ctccagcggc cagctcgtag aggccggggc gccgcaccgc tcccgcggca
       61 tgggcgagca cggtgtctcc gttcgcgacg atcggcaagg acgaggcccc cgggagcgcc
      121 gtcgtggccg cgggcaccag tggtagcccc gcctcggcgg cgggcggggc cggcgaccgt
      181 agccacagcc agcccagcag ggcgacgagg cagaccgcga agccgagcag tgtggcccgg
      241 ggtgagaggc cgaggtcggc caggagggcg aggagccggt ccgtcgggcg aaggggggcg
      301 acggggcggt tcagcccgac agggtcggca ggatcggtgg gctccgcagg cacggcagtc
      361 ctccacggct gggcagcacg cgggggaagc gcagagggat gttagcccct ttcaatccct
      421 tttacgccga agccaggctc agaagaggcg ttgcgacaac gacttgcggt aggccgcggt
      481 gcgggggtcg tcggggccga ggagctcgag caggtcgacg aactcctggc gggcgtcgtc
      541 gtccgctttg acccggtcga gcaggccgtc gaggcgaccg ctgatctcat caccgtcgac
      601 cacggcctcg cccgtgcggg ccttcgccgc gacccggcgg gtctcggggg actcggggat
      661 ccgggtcagc agctccaggg cctcgtcggc ccgaccgccg tcgacccaca ggtcggcgag
      721 cgcgaccaca gcccgctcgt cgtcgggcac gagggccagg acctggtcga gggacgcctc
      781 gtcgcctgcg gccagcagcg cctcgatggt gttctcggtc tcgctgggca gcaggccatc
      841 gacgaacgcc tggacggcat cctccccctg ggccccgatg aagctgtcca cgacggcacc
      901 gtccttgagc gcgtagacgg ccgggatcga ctggacctgg aaggcctggg acacctgggg
      961 gttctcgtcg atgtcgacct tgaccagctc gaccttgccg tcggtcgccc cgatcacctt
     1021 ctccaggatg ggtcccagct gcgtgcaggg tccgcaccag ggggcccaca ggtcgaccac
     1081 gacggggact acgtccgagc ggtcgaggac ggcggtctgg aaggtggcat cggtgacgtc
     1141 ggtcatcggt tcatccaacg gggtgagggg tcggggcatt ccggtgtagc agtaggttgc
     1201 tggcgatggc gaacaccgac gaagccgaga cgatcccggt cgaggggatc cacccggagg
     1261 aggtgaccga ctggttcgcc acccacgtcc ccgaggccca accgcccctc gccttcgacc
     1321 tcctcgccgg cggccgctcc aacctgacct actcggtcac cgaccgcacc ggcgcccgct
     1381 gggtgttgcg ccgcccaccg ctcggccatg tcctggccac ggcccacgac atgggtcggg
     1441 agcaccgcat catctcggcg ctggccccga ctccggtccc ggtggccccc gtcatcggcc
     1501 tcagcccgga cgactcggtg aacggggccc ccttctacgt catggccttt gtcgacggcc
     1561 ccatcgtgcg caacgccgaa ggggcccgtc agctccccgt cgagcaccgg gcccgggcca
     1621 ccgagtccat cgtcgacgtg ctggccgata tccacgccgt cgacgtcgaa gcggtggggc
     1681 tgggcgacct gggccgccac gagggctaca tcgaacggca gctcaagcgg tggcaggggc
     1741 agtgggagaa gtcgaagacc cgggagctcc cggtcatcga cgccgtgcac gaccgcttgg
     1801 ccctctcggt accggggcag gtcggcgtgg ccatcgtcca cggcgactat cgcctggaca
     1861 actgcgtcct cgacgacgag gggaccatcc aggccgtgct cgactgggag ctctgcaccc
     1921 tgggcgatcc cctggccgac gtcggccagc tctgcatgta ctggacggag cccggcgaca
     1981 ccctggtcgc cgctcctgac gccccgacca ccgtcgaggg gttcggcacc cgggccgacc
     2041 tcctggcccg ctacgccagc acctccggcc gggacctggc cgagatcgac tactacatcg
     2101 ccttcggctt ctggaagctg gcctgcatct gtgagggcgt gctcaagcgc taccaggccg
     2161 gggtcatggg tggccccgac gccagcgcgc tggtcgagat gttcacccac cacgtggcca
     2221 acctggccaa cgcggcggcc acgaccctgg ggatggaccc gtgagccacc cgctctacga
     2281 gttccaccag gacccggggc tgtccgagcc cgtcctgttg gtggccctgg agggctggat
     2341 cgacgccggt ggcggtgccg cctcggcggc ggagaccatc ctggcctcgg ccgaacagat
     2401 cccggtggcc acgttcaaca gcgacaccct cctcgaccac cgggcccggc gcccgatcat
     2461 gcacctggtc gacggggtca acacgggact cacctggccg acgattcgcc tggacgagac
     2521 ctgggaccga gaggggcggg acgtcctctt cctccacggc ccggagcccg accacaactg
     2581 gccgtcgttc gccaaggccg tcatcgagct cgccgagggc ttcgaggtca gcgaggtcta
     2641 cgggctcggg gcgtaccccg cccccgtccc ccacacccgg cccgtgcagc tggcctccac
     2701 ggcgtcgtcc gacgacatgg cccagcgggt cggcttcatc cggggcaccc tcgaggtgcc
     2761 cgccggggtg gccgcggcca tcgaggcgca ggcggccgag atcggcatgt ccgccctcgg
     2821 cctctgggcc caggtccccc actacgccgc ggccatgccc taccccgccg cagccgttcg
     2881 cctcatcgac ggcctcttcg aggtcgccgg gctgcgcttc gaccacggcg agctggatgc
     2941 ggaggtcgaa acgatccggg cccgactcga cgagctcgtg gccaacagcg acgagaacca
     3001 ggaactggtc caccagctag agatccacgt cgaccagagc gagcccgacc ccgacgacgg
     3061 cgggatcgaa ctgcgctccg gtgacgagct ggccgc
//
LOCUS       DASTFV010000187         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_696862, whole genome shotgun sequence.
ACCESSION   DASTFV010000187 DASTFV010000000
VERSION     DASTFV010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696862"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFP02_03520"
     CDS             complement(<1..249)
                     /locus_tag="VFP02_03520"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HET9542124.1"
                     /translation="MTTVTGLYRYPVKSMQGLDVTTLDLTADGFAGDRRWALLDAATG
                     RLMSAKRWSALLQASADDDGMTLPDGTWIPFGAPDADAI"
     gene            complement(246..1361)
                     /locus_tag="VFP02_03525"
     CDS             complement(246..1361)
                     /locus_tag="VFP02_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9542125.1"
                     /translation="MAEPAQALVLEAPRRLVAVERDRPVSMADTGVLRVEACGLCGTD
                     HEQYTGALHPGWAFVPGHEVVGVVEGVGAEAARRWGVAEGDRVALEVFLGCGECPACG
                     AGDVRHCVRHGLRTMYGFLGVEEGTGLSGGYATHLELRPDSRLLKVPDGLDPVLATAF
                     NPLGAGLRWGARLPGTRPGDVVAVLGPGIRGLCVVAAAREAGAGFVMVTGAGERDADR
                     LAWARRFGADLTVDVTEEDPVVALRRASGGRADVVVDVTAIAPTAFAQAVALCRTGGT
                     VVVAGTRGATTEAPGFHPDDVVGRELRVLGALGVDTADYAAALDLLATGRYPFVDVPR
                     EVVDLDGAADLLDRMAGGSGPPPPVHGVVVPDLRSGP"
     gene            1372..1809
                     /locus_tag="VFP02_03530"
     CDS             1372..1809
                     /locus_tag="VFP02_03530"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET9542126.1"
                     /translation="MADELLSEDEQNRRRQAIKGVFPATPFMGLLGIAFDRYEPDDVV
                     VRLPFHEQLTNDGEVYHGGVIATMLDTAGAAAAWSNHDFDKGSRASTVALSVQYVGAA
                     RRSDLVCHGRAIRRGRELIFCEVTAADDDGKVVAHALQTYRIV"
     gene            1901..2182
                     /locus_tag="VFP02_03535"
     CDS             1901..2182
                     /locus_tag="VFP02_03535"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HET9542127.1"
                     /translation="MLHHVVLFQVPDPLHREELVRRLQALPGLIPEIDAYEVGVDVSR
                     GDNSYDVGLYSTFADTDALEVYRAHHAHQAVLQLIGDISPHRIVTDWLD"
BASE COUNT          330 a          829 c          760 g          264 t
ORIGIN      
        1 gatcgcatcc gcgtcgggcg ccccgaacgg gatccaggtg ccgtcgggca gcgtcatgcc
       61 gtcgtcatcg gccgacgcct ggagcagcgc cgaccagcgc ttggcgctca tcagccgacc
      121 agtggcggca tcgagcaggg cccagcgccg gtcccccgcg aagccgtcgg ccgtgaggtc
      181 cagggtggtc acgtccaacc cctgcatcga cttcacggga tagcggtaga ggccggtcac
      241 cgtcgtcatg ggccggaccg taggtccgga acgaccactc cgtggaccgg cgggggcggc
      301 cccgacccac ccgccatgcg atccaggagg tcggcggcgc cgtcgaggtc gaccacttcc
      361 cggggcacgt cgacgaacgg gtaacggccc gttgccagca ggtcgagggc ggcggcgtag
      421 tcggcggtgt cgacgccgag tgccccgagg acccgcagct cgcgaccgac gacgtcgtcg
      481 gggtggaagc ccggggcctc ggtggtcgca ccccgcgtgc ccgccaccac cacggtgccg
      541 ccggtgcggc acagggccac ggcctgggcg aacgcggtcg gggcaatggc cgtgacgtcc
      601 acgacgacgt ccgcccggcc gccgctggcc cggcggagag cgacgaccgg gtcctcctcg
      661 gtcacgtcca ccgtcaggtc ggcaccgaag cggcgggccc aggccaggcg gtcggcgtcc
      721 cgctcccctg ctccggtgac catcacgaag ccggcgccgg cctcccgtgc ggcggcgacc
      781 acgcacaggc cacggatccc cggaccgagg acggccacga catcaccggg acgggtgccg
      841 ggcaggcggg caccccaccg gagccccgcg ccgagggggt tgaacgccgt ggccagcacg
      901 gggtcgagcc cgtcggggac cttcagcagc cgggagtcgg gccgcagctc cagatgggtc
      961 gcgtagcccc ccgacaggcc agtgccctcc tcgaccccga ggaagccgta catggtgcgc
     1021 agcccgtgac gaacgcagtg ccgcacgtcg cccgcgccac acgccgggca ctcaccgcag
     1081 ccgaggaaga cctcgagggc gacccggtcg ccctcggcca cgccccaccg acgggccgcc
     1141 tcggcgccga cgccctcgac cacgccgacg acctcatggc cgggcacgaa ggcccaaccg
     1201 gggtgcaggg cgcccgtgta ctgctcgtgg tcggtgccgc acaggccgca cgcctcgacc
     1261 cgtaggacgc cggtgtcggc catggacacg ggtcgatctc gctcgacggc cacaaggcgg
     1321 cggggcgcct ccaggacgag ggcctgcgcc ggctcagcca cactggtgac catggccgat
     1381 gagctcctgt ccgaggacga gcagaaccgg cggcggcagg cgatcaaagg cgtgttcccg
     1441 gcgaccccgt tcatggggct cctggggatc gccttcgacc gctacgagcc cgacgacgtg
     1501 gtcgtgcggc tcccgttcca cgagcagctc accaatgacg gcgaggtcta ccacggaggc
     1561 gtcatcgcca cgatgctgga caccgccggc gccgccgcgg cgtggtcgaa ccacgacttc
     1621 gacaagggct cgcgtgcgtc gacggtcgcc ctgtcggtcc agtacgtcgg ggctgcccgc
     1681 cgctcggacc tggtgtgtca cggccgggcg atccgccggg gccgggagct gatcttctgc
     1741 gaggtcaccg ccgccgacga cgacggcaag gtcgtggccc acgccctcca gacctaccgc
     1801 atcgtctgac ccgtaccatc cggcttcagc ggaagaaggg gttccgggga tcgcgggatc
     1861 cacgaccgga ccgtagaacg cgggcggcca gaatggcggc gtgctccacc atgtcgtctt
     1921 gttccaggtc cccgatcccc tccatcggga ggagctggtt cgccggctcc aggccctacc
     1981 cgggctgata cccgagatcg acgcctacga ggtcggcgtc gacgtctccc gcggcgacaa
     2041 cagctacgac gtcggcctct actccacgtt cgccgacacc gacgccctgg aggtctaccg
     2101 ggcccaccac gcccaccagg ccgtgctcca gctcatcggc gacatcagcc cccaccgcat
     2161 cgtcaccgac tggctcgact gac
//
LOCUS       DASTFV010000188         2960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_696906, whole genome shotgun sequence.
ACCESSION   DASTFV010000188 DASTFV010000000
VERSION     DASTFV010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696906"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1622)
                     /locus_tag="VFP02_03540"
     CDS             complement(30..1622)
                     /locus_tag="VFP02_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9542128.1"
                     /translation="MGDWGVRPVPTDLARRYRDEGWWNDDTVGRVLAEGLARQPDQRL
                     LFFSDDRPFRGTFADGLTMAQRVAGALAARGVRPGDPVAFQLPNHVEAAATFWGAALL
                     GAVVVPIVHFYGAKEVGYILRQSEARVLVTAAGTGHLDHLAILDGLAPDLPALEHVLV
                     VGDDAGRWEGFADAVDAATPIVELAAVDAAAPALIAYTSGTTADPKGVVHSHRTLGAE
                     IRQLAALQPDHGRPTLTGAPVGHAIGMLGGLLLPVFRGEPIAMIDGWDPATILRLMLE
                     HDLTAGSGSTYFLLSLLEHPDFGDAHVRHMPAIGLGGSAVPAAIGERAAGLGIETTRA
                     YGSTEHPSTTGARHSEPRAKRITTDGRPLAGVELCLDEDGEIWSRGPDLFSGYTDPQL
                     TAEATDADGWYRTGDIGVLDDEGWLTITDRAKDVIIRGGENISPAEVEDLLLRMPGVA
                     EVAVVAAPDERLGEHGCAFIRPTAGMAAPDLEAIRAHLEAAGLARPKWPEEIRTVTDF
                     PRTATGKVQKFALRDELRRSGG"
     gene            1666..2007
                     /locus_tag="VFP02_03545"
     CDS             1666..2007
                     /locus_tag="VFP02_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET9542129.1"
                     /translation="MADEDWTFPFEGPLHESFPALHEAQSSWLAQIDSLPAPDRKTHE
                     LIRLVCTVILRNAEGVERHARLAAEVGATWDEVLGAMMLTTPGFGVLPAVQAMPVARR
                     GFESAEPPEAD"
     gene            2015..2428
                     /locus_tag="VFP02_03550"
     CDS             2015..2428
                     /locus_tag="VFP02_03550"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HET9542130.1"
                     /translation="MTTPRPDFRILPRIRDDNEFFWTSGADGRLRFMRCGSCGFWIHP
                     SGPVCPVCHSRDLAPQAVAGEAVLHTYTVNHQAWLPGFEPPYVIAIVELPEQEGLRLT
                     TNLVNCALEDVQIGMPVRVLFEQWEDVWLPLFEPA"
     gene            2425..2595
                     /locus_tag="VFP02_03555"
     CDS             2425..2595
                     /locus_tag="VFP02_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542131.1"
                     /translation="MSGLPAQAQSQDLTVPIAVIVVCAVVIVLTLILQRVRARRRPPA
                     EEDLRSGSEVPQ"
     gene            2606..>2960
                     /locus_tag="VFP02_03560"
     CDS             2606..>2960
                     /locus_tag="VFP02_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9542132.1"
                     /translation="MRLRYSDEDEAFRAELLDWLAQNPPPTPEERTGRRSSADMPAWA
                     KAWQRTLFDGGWLVPGFPPERGGRNATAVQQMIYFEVFSDLGIPRTVNPQGLSIVAAS
                     LLDYGTEDQIERWALP"
BASE COUNT          394 a         1053 c         1086 g          427 t
ORIGIN      
        1 cggtcgattc ggtggtcggg acgaccagat caaccgccgg aacggcggag ctcgtcgcgg
       61 agggcgaact tctggacctt tccggtggcg gtgcgcggga agtcggtcac ggtgcggatc
      121 tcctccggcc acttcgggcg ggccaggccg gcggcctcga ggtgggcccg gatggcctcg
      181 aggtcggggg cggccatccc ggcggtgggc cggatgaacg cacagccgtg ctcgccgagg
      241 cgctcgtcgg gcgccgccac cacggcgacc tcggccacgc cgggcatgcg caggagcagg
      301 tcctcgacct cggccgggct gatgttctcg ccgccccgga tgatcacgtc cttggcccga
      361 tcggtgatgg tcagccagcc ttcgtcgtcg agcacgccga tgtcaccggt gcggtaccag
      421 ccgtcggcgt cggtcgcctc ggcggtgagc tggggatcgg tgtaacccga gaagaggtcg
      481 ggccctcggc tccagatctc gccgtcctcg tccaggcaga gctccactcc cgccaggggg
      541 cgcccgtcgg tggtgatgcg cttggcccgg ggctcgctgt ggcgcgctcc cgtggtggag
      601 gggtgctcgg tcgacccgta ggcgcgggtc gtctcgatgc cgaggccggc ggcccgttca
      661 ccgatggccg cgggtacggc cgagcccccg aggccgatgg cgggcatgtg tcgaacgtgg
      721 gcgtcaccga agtcagggtg ctcgaggagg ctgagcagga agtaggtcga tccgctgccg
      781 gcggtcaggt cgtgctccaa catcaaccgg aggatggtgg ctgggtccca gccgtcgatc
      841 atggcgatgg gctcgccccg gaagacggga aggaggaggc cgcccaacat cccgatggcg
      901 tggcccaccg gcgccccggt cagggtgggg cggccgtggt ccggctgcag ggcggcgagc
      961 tggcggatct cggcacccag ggtgcgatgc gagtggacca cgcccttggg gtcggctgtc
     1021 gtccccgagg tgtaggcgat gagggccgga gccgccgcgt ccacggcggc gagctcgacg
     1081 atcggggtcg cggcgtcgac ggcgtcggcg aagccttccc agcgaccggc atcgtcgcct
     1141 acgaccagca cgtgctcgag ggcgggcagg tccggagcca gcccgtcgag gatcgcgagg
     1201 tggtcgaggt ggcccgtgcc ggccgcggtg accagaaccc tggcctcgga ctgacggagg
     1261 atgtagccga cctccttggc gccgtagaag tggacgatgg gcacgaccac ggcgcccagc
     1321 agcgccgccc cccagaaggt cgcggcggcc tccacgtggt tcggcagctg gaaggcgacg
     1381 ggatcacccg ggcgcacgcc ccgggccgcc agtgcgccgg cgacccgctg ggccatggtc
     1441 aggccgtcgg cgaaggtccc ccggaacggt cggtcgtcgg agaagaacag gaggcgctgg
     1501 tccggctgcc gagcgaggcc ctcggccagg acccgcccga ccgtgtcgtc gttccaccag
     1561 ccctcatccc ggtaccgccg ggccaggtcg gtcggcacgg gacggacccc ccagtccccc
     1621 atggcccgtg acggtagacc acacctcggg gcctacgatc ggcccgtggc cgatgaggac
     1681 tggaccttcc ccttcgaggg tcccctgcac gagtcgttcc ccgccctgca cgaggcccag
     1741 agctcgtggc tggcccaaat cgactcgctg ccggcccctg accgcaagac ccacgagctg
     1801 atccgcctcg tctgcacggt catcctccgc aacgccgagg gcgtcgagcg ccacgcccgg
     1861 ctggccgcgg aggtgggcgc gacctgggac gaggtcctgg gggcgatgat gctgaccacg
     1921 cccggcttcg gcgtcctgcc cgccgtccag gccatgccgg tcgcccgccg gggcttcgag
     1981 tcggccgagc cccccgaagc cgactgaggt gggcgtgacc accccgcggc ccgacttccg
     2041 gatcctgccg cggatcaggg acgacaacga gttcttctgg acgtcagggg ccgacggtcg
     2101 gctgcgcttc atgcgctgcg ggtcctgcgg cttctggatc cacccctcgg gccccgtgtg
     2161 ccccgtctgt cattcgcggg acctcgcccc ccaggcggtg gcgggggagg ccgtcctcca
     2221 cacctacacg gtgaaccacc aggcctggct gccgggcttc gagccgccgt acgtgatcgc
     2281 gatcgtggag ctcccggagc aggaggggct tcgtctcacc accaacctgg tcaactgtgc
     2341 cctcgaggac gtgcagatcg ggatgccggt gcgggtcctc ttcgagcagt gggaggacgt
     2401 ctggttgccg ctcttcgagc cggcatgagc ggtctgcccg cccaggccca atcccaggac
     2461 ctcaccgtcc cgatcgcggt gatcgtcgtc tgcgccgtcg tcatcgtgct gaccctgatc
     2521 ctgcagcggg tccgggcccg acggcgcccc ccggccgagg aggacctgcg ttcggggtcc
     2581 gaggtcccgc agtaggttcg gggcgatgcg gctccggtac tccgacgagg acgaggcgtt
     2641 ccgggccgag ctcctggact ggctggccca gaacccgccg ccgacgcccg aggagcgcac
     2701 gggacggcgg tccagtgccg acatgcccgc gtgggccaag gcgtggcagc gcacgctctt
     2761 cgacggcggc tggttggtcc ccggcttccc gcccgaacgg ggcggccgca acgccaccgc
     2821 ggtccagcag atgatctact tcgaggtctt ctcggatctc ggcatccccc ggaccgtcaa
     2881 cccccagggc ctgtcgatcg tcgccgcctc tctcctcgac tacggcaccg aggaccagat
     2941 cgagcgctgg gccctgccca
//
LOCUS       DASTFV010000189         5766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_703579, whole genome shotgun sequence.
ACCESSION   DASTFV010000189 DASTFV010000000
VERSION     DASTFV010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703579"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1482)
                     /locus_tag="VFP02_03565"
     CDS             complement(<1..1482)
                     /locus_tag="VFP02_03565"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HET9542133.1"
                     /translation="MRRLLAAAALVAALLLLGAAPASAHAVLEASTPGDGARLDTPPE
                     SVRLEFNEPVSADLGGLRVFSADGDRVDVGETRADGEVVEVDLQDDLADGAYVATYRV
                     VSDDGHPVQGGLVFSVGDVEADPALINQFFDEGSDRAWEIAGAVFRFLAYAGVLLAAG
                     GAFFLAFVHDGGPEEDRLRGLVRRAAVVGAVGILAALPIQAALGTGQGFGAITQDGVL
                     GEVLQEGVGLATFLGLLGLVLLVLGGTRRAVIGAGVLLAAGSFALSGHTRSTDNELLA
                     VSAAIVHTVTAAVWFGGLVLLAVILWGRRAEEDHASSAGMVGRFSRVATVCVLAVGAA
                     GVALSWSEVRALRALTSTTYGWTLLAKVGVVLVVGLIGAHNRFRVVPAIQRSPSATRA
                     WSLLRKSVRLEAAALVVAIALTAVLVNVTPARSAAGIGEIFSETVALGDAGSVNIVVD
                     PNQAGRNAIHLYFFDEEGRPAEIADDVSVSLSLPSDDIGPIVRE"
     gene            complement(1479..2081)
                     /locus_tag="VFP02_03570"
     CDS             complement(1479..2081)
                     /locus_tag="VFP02_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET9542134.1"
                     /translation="MRCDQSRDVLSARLDGEATADELAVLDRHLASCAACTDFASSLM
                     ALDRVTRLTPAEPVPDLAGAVMAANPPVRRDPRREAARWSLVVVALAQLVIALPSLLT
                     GPSGEAVHTTRELGSWSAALAVGLLVAAWQPARARGMLPLGLVLAGVLSLGALVDIIS
                     GATAGAGEAVHLLEVAGVALLWLLAHRDDDARATQPVLAR"
     gene            2132..2674
                     /locus_tag="VFP02_03575"
     CDS             2132..2674
                     /locus_tag="VFP02_03575"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9542135.1"
                     /translation="MPDDQLTELARQAAGGDRVALSDLVRGTQADVWRLCAHLVDRQS
                     ADDLTQDVYARAITGLPHFRGESPVRLWLLGIARHVCIDEVRRRTRRRRILARNEVEA
                     IETVADPTGAVDLDELLAGLDPDQRAAFVLTQVLGLRYAEAAEAVGCPVGTIRSRVAR
                     AREHLVAAVVAPAESDRSGT"
     gene            complement(2722..>5766)
                     /locus_tag="VFP02_03580"
     CDS             complement(2722..>5766)
                     /locus_tag="VFP02_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HET9542136.1"
                     /translation="YGVVRFAEAARAYGLPTVFGAELALGMTRPQMGEPDPEGDHLVV
                     LARDPTGYARLGTAISAAQMAGEKGAPRTSLSALAGDAVARDHWLVLTGCRKGAVPRA
                     LVERGPSAAQRELARLVDAFGRENVAIELWDHGDPLDSARNDGLAEVAVRAGVDVVAT
                     NNVHYATPSRRRLATALAAVRARRSLDEIDGWLPAAAGAHLRSGDEQARRFARYPGAV
                     DRAAALGRECAFDLQLVAPNLPPFPCPPGQDEMSFLRQLTEQGALGRYGTRAEHPAAY
                     QQIDHELDMIEALGFPGYFLVVWDIVRFCRENGIFCQGRGSAANSAVCYALGVTNADA
                     VKLGLLFERFLSPERDGPPDIDIDIESDRREEAIQYVYERHGRANAAQVANVITYRAR
                     SAVRDMAKALGHAPGQQDAWAKQIDRWSHVADSEHQRGPDGHVDHDIPAEVLVLAAEI
                     EDFPRHLGIHSGGMVMCDRPVAEVCPVEWGRMENRSVLQWDKDDCAAVGLVKFDLLGL
                     GMLSALRYCIELVAEHEGAQLDLATLPHEDPEVYAMLCAADTVGVFQVESRAQMATLP
                     RLKPRTFYDLVVEVALIRPGPIQGGSVHPYIRRRNGQEAVTYLHPLLEKSLQKTLGIP
                     LFQEQLMQMAIDVAGFTASEADELRQAMGSKRSVARMERLRGRFFAGMAERGITQPVD
                     EKIWEKLAAFANFGFPESHSVSFAYLVYASSWLKRYHPAAFCAALLNAQPMGFYSPHT
                     LVQDARRHGVEIRTPDVNASAARATLQVGSTGAPGRDPSTWGDGGPVVRLGIGAVRGI
                     GEDLALAIAAGRPYGDPEDLARRTGCSLAQLEALATAGAFGCFGLERRSALWGAGAVA
                     QGGADRLAGVVTGVTAPMLPGMEPREESLADLWATGISPDGHPTRFVREELDRRGVVT
                     AIALKAMPDGVRVQVGGVVTHRQRPATASGITFLNLEDETGLVNVVVSKGCWARYRKV
                     ARATPALIVRGTLEQVEGVTNVVADKLEPLPVAAPQRSRDFR"
BASE COUNT          827 a         2114 c         2125 g          700 t
ORIGIN      
        1 ctcccgcacg atggggccga tgtcgtcgga cggcagcgac aggctgacgc tgacgtcgtc
       61 ggcgatctcc gccggccggc cctcctcgtc gaagaagtag aggtggatgg cgttgcggcc
      121 cgcctgattc gggtcgacga cgatgttcac cgagccggcg tccccgaggg ccacggtctc
      181 gctgaagatc tcgccgatgc ccgcggcact gcgggccggg gtgacgttca cgagcactgc
      241 ggtgagggcg atggccacga cgagagctgc ggcctcgagg cggacgctct tgcggagcaa
      301 cgaccaggcc cgggtggccg acggcgagcg ttggatggcg gggacgaccc ggaaccggtt
      361 gtgggccccg atgaggccga cgaccaggac cacgccgacc ttggccagca gcgtccagcc
      421 gtaggtggtg gaggtcagcg cccgcagggc ccggacctcg gaccaggaca gagcgacccc
      481 ggcggcaccg acggcgagga cgcagaccgt ggccacccgg gagaagcggc ccaccatccc
      541 ggctgaggac gcgtggtcct cctcggcgcg gcgaccccac aggatcacgg ccaggagcac
      601 gaggccgccg aaccacacgg cggcggtcac ggtgtggacg atcgccgcgc tcaccgcgag
      661 gagctcgttg tcggtgctgc gggtgtggcc gctgagggcg aacgaaccgg ccgcgagcag
      721 cacgccggct ccgatcaccg cccggcgcgt ccccccgagc acgagcagca ccaagccgag
      781 caggccgagg aacgtggcca ggcccacccc ctcctgcagg acctcgccca gcacgccgtc
      841 ctgggtgatg gcgccgaagc cctggcccgt gcccagcgcg gcctggatcg ggagggcggc
      901 caggatcccc acggcgccga cgaccgcagc gcggcggacg aggccccgca gacggtcctc
      961 ctcgggcccg ccgtcgtgga cgaaggccag gaagaaggca ccacccgcgg ccaacaacac
     1021 gccggcgtag gccaggaagc ggaagacggc gccggcgatc tcccaggccc ggtccgagcc
     1081 ctcgtcgaag aactggttga tcagcgccgg gtcggcttcg acatcgccga cgctgaagac
     1141 caggccgccc tggaccggat ggccgtcgtc ggacacgacc cggtaggtgg cgacgtacgc
     1201 gccgtcggcc aggtcgtcct gcaggtcgac ctcgaccacc tcgccgtccg cgcgggtctc
     1261 gccgacatcg acccggtcgc cgtcggctga gaacacccgc agcccgccca gatcggccga
     1321 cacgggctcg ttgaactcga gacggacgct ctcaggtggg gtgtcgaggc gggcaccgtc
     1381 gcccggggtc gacgcctcca ggacggcgtg ggccgacgcc ggggcggcac cgagcaggag
     1441 gagcgccgcg accagggccg cggccgcgag cagtcgcctc accgggcgag gaccggctgg
     1501 gtcgcacggg catcgtcatc acggtgggcc aggagccaga gcagggccac gccggccacc
     1561 tcgagcaggt ggacggcttc accggccccg gcggtcgccc ccgagatgat gtcgaccagg
     1621 gccccgaggc tcaggacccc cgccaacacg agccccagcg ggagcatccc ccgggcccgg
     1681 gcgggctgcc aggcggcgac gaggagcccg acggccagcg ccgcggacca gctccccagc
     1741 tcgcgggtcg tgtgcacggc ttcaccgctc gggccggtca gcagcgacgg aagggcgatg
     1801 acgagctgag ccagggcgac gaccacgagg ctccatcggg cggcctcccg ccggggatcg
     1861 cggcgcacgg gcgggttggc ggccatgacc gcgccggcca ggtcggggac gggctcagcc
     1921 ggcgtcaggc gggtgacccg gtcgagggcc atgagcgagg aggcgaagtc ggtgcaggcg
     1981 gcacagctgg caagatgacg gtccagcacg gccagttcgt cggccgtggc ctcaccgtcc
     2041 agccgggcgg acagcacatc tcgactctga tcacaacgca tgccctacct gtcgaaccaa
     2101 ccccccgtct ggttcccggc gtgggcccga gatgcctgac gaccagctga ccgaactggc
     2161 ccggcaggcc gccggtggtg accgggtggc gctgtccgac ctcgtccggg gcacgcaggc
     2221 cgacgtctgg cggctgtgcg cccacctcgt cgaccgccag agcgccgacg acctgaccca
     2281 ggacgtctac gcccgggcca tcaccggtct cccccacttc cggggggaga gcccggtgcg
     2341 cctctggctc ctcggcatcg cccgccacgt ctgcatcgac gaggtccgcc gccggacccg
     2401 ccgccgacgg atcctggccc gcaacgaggt cgaggccatt gagacggtcg ccgacccgac
     2461 cggggccgtc gacctcgacg agctgctggc cggcctcgac cccgatcagc gcgccgcctt
     2521 cgtcctgacc caggtcctgg gcctgcgcta cgccgaagcg gccgaggccg tgggctgccc
     2581 cgtcggcacc atccgcagcc gcgtcgcccg cgcccgtgaa cacctcgtcg ccgccgtcgt
     2641 cgcccccgcc gaatccgacc gttctggaac ctgaagctcg cggcccgtca ccaacgacag
     2701 gttccagaac gatcgggggg gtcagcggaa gtctcggctg cgttgcgggg cggcgacggg
     2761 gaggggctcg agcttgtcgg ccaccacgtt ggtgacgccc tcgacctgct ccagcgtccc
     2821 ccgcacgatc agggccgggg tggcgcgggc caccttgcgg tagcgggccc agcagccctt
     2881 ggagacgacc acgttgacca ggccggtctc gtcctccagg ttgaggaagg tgatgcccga
     2941 ggccgtggcc gggcgctgac ggtgggtgac cacgccaccg acctgcaccc ggacgccgtc
     3001 gggcatcgcc ttcagggcga tggcggtgac cacgccccgc cggtccaact cctcgcggac
     3061 gaagcgggtg gggtggccgt cggggctgat gccggtggcc cacaggtcgg ccagggactc
     3121 ctcgcggggc tccatgcccg ggagcatcgg cgccgtcacc ccggtgacca ccccggccag
     3181 gcggtcggcg ccgccctggg ccacggcccc ggccccccag agggccgagc ggcgctcgag
     3241 gccgaagcag ccgaaggctc cggccgtggc cagggcctcc agctgggcca ggctgcaccc
     3301 cgtccggcgg gccaggtcct cagggtcgcc gtagggccgc ccggcggcga tggccagggc
     3361 caggtcctcc ccgatccccc gcaccgcccc gatccccaac cgcaccaccg gccccccgtc
     3421 accccaggtc gacgggtcac ggcccggggc gccggtcgag cccacttgga gggtggcccg
     3481 ggcggcgctg gcgttgacgt cgggggtgcg gatctcgacg ccgtggcgac gggcgtcctg
     3541 gaccagggtg tggggcgagt agaagcccat gggctgggcg ttgagcaggg cggcgcagaa
     3601 ggcggccggg tggtaccgct tcaaccagga cgaggcgtag accaggtagg cgaagctcac
     3661 cgaatggctc tcggggaagc cgaagttggc gaaggcggcc agcttctccc agatcttctc
     3721 gtccaccggc tgggtgatcc cccgctcggc catcccggcg aagaaccggc cccgcagccg
     3781 ctccatgcgg gccacgctgc gcttggagcc catggcctgg cgcagctcgt cggcctctga
     3841 cgcggtgaag ccggccacgt cgatggccat ctgcatgagc tgctcctgga acagcggtat
     3901 gcccagggtc ttctgcagcg acttctcgag cagggggtgc aggtaggtca ccgcctcctg
     3961 accgttgcgg cggcggatgt aggggtgcac cgacccgccc tggatgggcc cggggcggat
     4021 gagggcgacc tcgaccacca ggtcgtagaa ggtgcggggc ttcaggcggg gcagggtggc
     4081 catctgcgct cggctctcga cctggaagac ccccacggtg tccgccgcgc acaacatggc
     4141 gtagacctcg gggtcctcgt gcgggagggt ggccaggtcc agttgggccc cctcgtgctc
     4201 ggccaccagc tcgatgcagt agcggagggc ggacagcatc cccaggccga gcaggtcgaa
     4261 cttgaccagg cccaccgcag cacagtcgtc cttgtcccac tgcaggacgc tgcggttctc
     4321 catgcgcccc cactcgaccg ggcagacctc cgccaccggc cggtcgcaca tgaccatgcc
     4381 cccggagtgg atgcccaggt ggcgggggaa gtcctcgatc tcggcggcca ggaccagcac
     4441 ctcggcgggg atgtcgtggt cgacatggcc gtcggggccc cgctggtgct cgctgtcggc
     4501 gacgtggctc cagcggtcga tctgcttggc ccaggcgtcc tgctggcctg gagcgtgccc
     4561 cagggccttg gccatgtccc gcaccgccga ccgggcccgg taggtgatga cgttggccac
     4621 ctgggcggcg ttggcccggc cgtggcgctc gtagacgtac tggatcgcct cctcccgccg
     4681 atcgctctcg atgtcgatgt cgatgtcggg tgggccgtcc cgctccgggg acaggaagcg
     4741 ctcgaagagc agacccagct tcacggcgtc ggcgttggtg accccgaggg cgtaacagac
     4801 cgcggagttg gccgccgacc cccgcccctg gcagaagatc ccgttctccc ggcagaagcg
     4861 cacgatgtcc cagaccacca ggaagtaccc cgggaacccg agcgcctcga tcatgtccag
     4921 ctcgtggtcg atctgctgat acgccgccgg gtgctcggcc cgggtgccgt accgcccgag
     4981 cgcgccctgc tcggtcagct ggcggaggaa ggacatctcg tcctggccgg gcgggcacgg
     5041 gaacggcggc aggttcgggg cgacgagctg caggtcgaag gcacactcgc ggcccagggc
     5101 cgccgcccgg tccaccgcgc cgggatagcg ggcgaagcgg cgggcctgct cgtcgcccga
     5161 gcgcaggtgg gcaccggccg cggcgggcaa ccagccgtcg atctcgtcga gggagcgccg
     5221 ggcccgcacc gcggccagag cggtggccag ccggcgacgg gagggcgtgg cgtagtggac
     5281 gttgttggtg gccaccacgt cgaccccagc ccggacggcg acctcggcca agccgtcgtt
     5341 gcgggcactg tccagcgggt cgccgtggtc ccagagctcg atggccacgt tctcccgccc
     5401 gaaggcgtcg accaggcggg ccagctcccg ttgggcggcc gacggccccc gttcgaccag
     5461 cgcccggggc accgccccct tgcggcagcc ggtcagcacc agccagtggt cgcgggccac
     5521 ggcgtcaccg gccagcgccg acagggaggt gcggggcgcc cccttctccc cggccatctg
     5581 ggccgcgctg atggccgtgc ccagccgggc gtagcccgtc gggtcccggg ccaggacgac
     5641 gaggtggtcg ccctcggggt cgggctcgcc catctgcggc cgggtcatgc ccagggccag
     5701 ctcggcgccg aagacggtgg gcagcccgta ggcccgggcc gcctcagcga agcggaccac
     5761 gccgta
//
LOCUS       DASTFV010000190         3118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_706594, whole genome shotgun sequence.
ACCESSION   DASTFV010000190 DASTFV010000000
VERSION     DASTFV010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706594"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1307
                     /locus_tag="VFP02_03585"
     CDS             147..1307
                     /locus_tag="VFP02_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542137.1"
                     /translation="MSLGPLVPADETFHHQITDTFATVGQSDRSWTEKVCAQAAARDG
                     SVQVHLGMGKYPNRGVVDAYAGVSRGIEQWTVRASRPLALDPDDTAVGPIRYEVLEPL
                     RSVRFALDANDVVPVAFEWTFEGAVPPALENREVHRGADGFRIDADIVRYHHTGVAHG
                     WVEVDGERTPIEPSTWFSTRDHSWGVRYMVGAPVDDVPTRGEVPGLASLIIWSPMLCQ
                     TDDGEPYALHVFHQQHAVGDYRQVTTQGGVEHPDGRRESFVAIVPELAFDPVNRRLRG
                     GVLHCTTAAGVERPLTITALDETGFHLGAGLYFGFDDQWHGQFRGRSLHLDGEHIGDC
                     TTVEAAHRLHQLRDTVIRVDDPVGGGTGWGNAQTMATGPHPDLGLTEDTSFV"
     gene            complement(1327..1752)
                     /locus_tag="VFP02_03590"
     CDS             complement(1327..1752)
                     /locus_tag="VFP02_03590"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HET9542138.1"
                     /translation="MSPEPEPIELVPLGRMTIGLRDPMLLPGTPGGTFVIAELASAHF
                     EGERLKADAKGSANADWLTIGADGTAAIDVRVLLETDDGALIYLQYAGRLNLADQTVI
                     AAPLFQTGDERYAWLNGVQAVAKGKTDGTTLVYDFHEVR"
     gene            1837..2616
                     /locus_tag="VFP02_03595"
     CDS             1837..2616
                     /locus_tag="VFP02_03595"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET9542139.1"
                     /translation="MPEGDTIHRVADRLRPALAGQALERFEAPRAVGPRPALGSAITQ
                     VEAKGKHLLIHFSDGVTLHTHLRMTGTWHLYRSGSRWRLPGHLARAVVGVADWEAVCF
                     KAPVVDLYREPPPHLPQPLDHLGPDLCLEDPPVEDVLARMAARADADSEIAPLLLDQQ
                     VAAGIGNVFKSEVLWACRVSPFARVQDLDVDTRRRLVTVAARQLRANVTAPGERSTLP
                     GGGLAVYGRLGQPCPRCGTPVQRRLQGEDPRVTYWCPRCQS"
     gene            2613..>3118
                     /locus_tag="VFP02_03600"
     CDS             2613..>3118
                     /locus_tag="VFP02_03600"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9542140.1"
                     /translation="MRVVIIGAGFGGLNAARELKGADVEVTLIDRNNFHTFAPLLYQV
                     ATSGLNAADVAHPVRGVFWKQPNVTFRNASVTGVDWEEQVVEVDDGDPVAFDGLIIAA
                     GAGTNFFGVPGAAEHSLPLYSLADALALRNHLLARVEWAHAHPDEADAALTWVIVGGG
                     PTGVEMAGA"
BASE COUNT          417 a         1145 c         1087 g          469 t
ORIGIN      
        1 gccagccacg ccacgaaccc ggcgaccacc ccgtcgaggt cccgctgcac cgtgagcgcc
       61 attcgctcat ggtggcacgg ggcccgctca gggcgggtcg gcgaccggct gacggccgcg
      121 gcccgggccc cttctagggt gccggcatgt cgctcggacc tttggtgccc gccgatgaga
      181 cctttcacca ccagatcacc gacaccttcg ccacggtcgg ccagtccgat cggtcctgga
      241 cggagaaggt ctgtgcccag gccgcggccc gcgacggctc tgtccaggtc cacctcggca
      301 tgggcaagta cccgaaccgc ggtgtcgtgg atgcctacgc cggggtctcc cgaggcatcg
      361 aacagtggac ggtgcgggcc agccggcccc tggccctgga cccggacgac acggccgtcg
      421 gacccatccg ctacgaggtc ctcgagccgc tccggtcggt ccgcttcgcc ctcgacgcca
      481 acgacgtggt gccggtcgcc ttcgagtgga ccttcgaggg ggcggtcccg cccgccctcg
      541 agaaccgcga ggtccatcgg ggggccgacg gcttccggat cgacgccgac atcgtccgct
      601 accaccacac cggtgtggcc cacggctggg tcgaggtcga cggcgagcgc acgccgatcg
      661 aaccgagcac ctggttctcc acccgggacc actcctgggg agtgcgctac atggtcgggg
      721 ctccggtcga cgacgtcccg acccggggcg aggtcccggg cctggcctcc ctgatcatct
      781 ggtcaccgat gctgtgccag accgacgacg gcgagccgta cgccctgcac gtcttccacc
      841 agcaacacgc ggtcggcgac taccggcagg tgacgaccca gggtggggtc gagcaccccg
      901 acggtcgccg ggagtccttt gtcgccatcg tccccgagtt ggcgttcgac cccgtcaacc
      961 ggcggctgcg gggcggcgtg ctgcactgca cgaccgcggc gggcgtcgag cgccccctca
     1021 cgatcacggc gctggacgag accggcttcc acctgggcgc cggcctctac ttcgggttcg
     1081 acgaccagtg gcacggccag ttccggggtc ggagcctcca cctcgacggt gagcacatcg
     1141 gtgactgcac gaccgtcgag gccgcccacc gcctgcacca gttgcgggac accgtgatcc
     1201 gggtcgatga cccggtgggc gggggcacgg ggtggggcaa cgcccagacg atggccaccg
     1261 gcccgcatcc ggacctcggc ctgaccgagg acacgtcgtt cgtctgaggc gatcgaggcc
     1321 ccgcgatcag cgaacctcgt ggaagtcgta gaccagggtg gtgccgtcgg tcttgccctt
     1381 ggcgacggcc tgcacgccgt tgagccaggc gtagcgctcg tcgccggtct ggaacagggg
     1441 agcggcgatg accgtctggt cggccaggtt cagccggccg gcgtactgca ggtagatcag
     1501 tgccccgtcg tcggtctcca agaggacccg cacgtctatg gccgcggtgc cgtcggcccc
     1561 gatggtcagc cagtcggcgt tggcgctgcc cttggcatcg gccttcaggc gctcgccctc
     1621 gaagtgggct gaggccagct cggcgatgac gaacgtgccg cccggtgtcc cagggagcag
     1681 catcgggtcc cggaggccga tggtcatgcg gcccagaggg accagttcga tgggctcggg
     1741 ttcggggctc atggtcgtgt cgtcctttcg tttggccggg cacgttaggc gcgggcgcgg
     1801 gccgctcccg ccggcggatg tccatagcct gaaggggtgc ccgagggcga caccatccac
     1861 cgggtggccg atcggctccg tccggcgctg gccggtcagg cgctcgagcg tttcgaggcc
     1921 ccccgcgccg tcgggccccg tcccgccctc ggttcggcca tcacccaggt cgaagccaag
     1981 ggcaagcacc tgctgatcca cttctcggac ggggtgaccc tccacaccca ccttcggatg
     2041 accggtacct ggcacctcta ccgctcgggg agccggtggc gcctgcccgg tcacctggct
     2101 cgggcggtgg tcggggtggc cgactgggaa gccgtctgct tcaaggcccc ggtggtcgac
     2161 ctgtaccgcg agccgccgcc ccacctgccc cagcccctcg atcacctggg ccccgacctc
     2221 tgtctggagg accctccggt cgaggacgtc ctggcccgga tggcggcccg ggccgatgct
     2281 gacagcgaga tcgcgccgct cctcctcgac cagcaagtgg cggccggcat cggcaacgtc
     2341 ttcaagagtg aggtgttgtg ggcgtgccgg gtcagcccgt tcgcccgggt ccaggacctc
     2401 gacgtcgaca cccgccgtcg cctcgtgacc gtggccgccc gccagctccg ggcgaacgtc
     2461 accgcgccgg gcgagcgctc gaccctgccc ggaggcgggc tcgccgtcta cgggcgcttg
     2521 ggacaaccgt gcccccggtg cggcacgccc gtccagcgtc gcctccaggg tgaggatccc
     2581 cgggtgacct actggtgccc ccggtgccag tcgtgagggt ggtcatcatc ggggccggct
     2641 tcggcgggtt gaacgccgct cgggagctga aaggggccga cgtcgaggtc accctcatcg
     2701 accgcaacaa cttccacacc tttgcgccgc tgctctacca ggtggccacc tccggcctga
     2761 atgcggccga tgtcgcccac ccggtccgtg gcgtcttctg gaagcagccc aacgtgacct
     2821 tccgcaacgc ctcggtcacg ggtgtggact gggaagagca ggtcgtggag gtcgacgacg
     2881 gcgaccccgt cgccttcgac ggcctgatca tcgccgccgg cgccgggacc aacttcttcg
     2941 gggtgccggg ggcggccgag cacagcctgc cgctctactc cctggctgat gcgctggccc
     3001 tgcgcaacca cctgctggcc cgggtcgaat gggcccacgc ccatcccgac gaggccgacg
     3061 ccgccctcac gtgggtcatc gtcggcggcg ggcccaccgg ggtcgagatg gccggcgc
//
LOCUS       DASTFV010000191         3029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_719511, whole genome shotgun sequence.
ACCESSION   DASTFV010000191 DASTFV010000000
VERSION     DASTFV010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719511"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VFP02_03605"
     CDS             complement(<1..634)
                     /locus_tag="VFP02_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET9542141.1"
                     /translation="MKRAAGLVVLALLAGGVAVSAAVRPPTEHVLLVGDSIMRQTGPA
                     LARQLGDGYTVHNEAVNGSGLLTPALFDWSDQLEQDLVRTDPDVVVFLFIGNYTADPA
                     EFWTTASGRRIRDVGSAAFTREWGRQADAAMAAIAETGAPVVLVLPPPMVSDELQAVV
                     DRLRVEYERVAADGPFVRLVDAADAVGGPNGEWVSQLPDADGRSLYVRVPD"
     gene            complement(631..795)
                     /locus_tag="VFP02_03610"
     CDS             complement(631..795)
                     /locus_tag="VFP02_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542142.1"
                     /translation="MAGEADEYEKMALASMRRASESGGDPRFHHARAQIFATLALAAA
                     SEAAKANNGK"
     gene            833..1621
                     /locus_tag="VFP02_03615"
     CDS             833..1621
                     /locus_tag="VFP02_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9542143.1"
                     /translation="MILDRFRLDGKAVIVTGAGRGIGAAIARTYAEVGANVVLAARTE
                     SQLDEVATDVRASGGEAVVVPCDVSELDTLDHLVATAVEAFGGIDVVVNNAGGSMPNS
                     FLDTSVRAFEKAFHFNVTTAFRLTQLAVPHMLGRDGASVVNISSAIGRMSDRGFAAYG
                     TAKGALTHLTRLLASDLAPRVRVNGIAVGSVATSALQVVLDSDEIHDEMVRRTPLQRL
                     GDPTDIAAAALWLASPAGDWVTGKVVEVDGGLESPNLAMGLPDL"
     gene            complement(1904..2809)
                     /locus_tag="VFP02_03620"
     CDS             complement(1904..2809)
                     /locus_tag="VFP02_03620"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HET9542144.1"
                     /translation="MRWKPGQGRSENIEDRRGSSAGGLGGLGGLIPGGGKGGGVIGIV
                     VLLAFVLLGKSCVSDGSGGDSASGGFDVEDINDILSQFSPAGGAAAGEDPLDPADDPD
                     ANTVDFVSFVLNDVQGFWYDYFDDAGENYPEAELVLFRNSVQSGCGPASAATGPFYCP
                     VDRKAYIDLAFFRELTTDRFGAPGDFAQAYVLAHELGHHVQNVLGIDPPSSNELSVRL
                     ELQADCFAGVWAHSAFQRDLLSDGDIEEGLDAAASVGDDRLGVDSPESFTHGSSEQRV
                     RWLRTGYETGDVQACDTFSVDESDL"
     gene            complement(2835..>3029)
                     /locus_tag="VFP02_03625"
     CDS             complement(2835..>3029)
                     /locus_tag="VFP02_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542145.1"
                     /translation="CDQLDDDQLQAFCDDGNVDACQVLLARQGDGEPDGPNGEGNGNG
                     NGNGNGNGNGNGNGNGDEGD"
BASE COUNT          429 a         1108 c         1023 g          469 t
ORIGIN      
        1 agtcgggtac ccggacgtag agcgagcggc cgtcggcatc cgggagttgg ctgacccact
       61 cgccgttcgg gccgcccacg gcgtcggcgg cgtcgaccag acggacgaac ggcccgtcgg
      121 ccgccacccg ctcgtactcg acccgcagcc tgtcgaccac cgcctgcagc tcgtcgctga
      181 ccatcggcgg cgggagcacg agcacgaccg gggcacccgt ctcggcgatc gccgccatgg
      241 ccgcatcggc ctgccgaccc cattcccggg tgaaggcggc cgatccgacg tctcgaatgc
      301 gccggccgct cgccgtggtc cagaactcgg cggggtcggc ggtgtagttc ccgatgaaga
      361 ggaagaccac gacgtcgggg tcggtgcgga ccaggtcctg ctcgagctgg tcggaccagt
      421 cgaagagcgc gggggtcagc aggccggagc cgttgacggc ctcgttgtgc accgtgtagc
      481 cgtcgccgag ctgacgggcc agggccggac cggtctggcg catgatcgag tcgcccacca
      541 acaggacgtg ctcggtgggc ggtcggacgg ccgcgctcac cgcgactccg ccggccagga
      601 gggccaggac gaccaggccg gccgctcgct tcacttgccg ttgttggcct tcgccgcctc
      661 gctggccgcg gccagcgcga gggtggcgaa gatctgggcc cgggcgtggt ggaacctggg
      721 gtcaccaccc gactccgagg cgcgtcgcat ggaggcgagc gccatctttt cgtactcgtc
      781 agcttcaccg gccatgggca cggcatgcct atcggcatga cacagtgtcg aaatgatcct
      841 cgatcgcttt cgacttgacg gcaaagccgt catcgtgacc ggcgccggac gaggcatcgg
      901 cgccgccatc gcccgaacct acgccgaagt gggggccaac gtggtcctcg ccgcccgcac
      961 cgagagccag ctcgacgagg tggcgaccga cgtccgagca tcgggcgggg aggccgtcgt
     1021 ggtcccctgc gacgtgagtg agctcgacac gctggatcac ctcgtcgcca ccgccgtcga
     1081 ggccttcggc ggcatcgacg tcgtcgtcaa caacgccggc ggctcgatgc ccaactcgtt
     1141 cctggacacc agcgtccggg ccttcgagaa ggcgttccac ttcaacgtga ccaccgcgtt
     1201 ccgcctgacg cagctggccg tcccccacat gttgggccgg gacggggcca gcgtggtcaa
     1261 catctcctcc gccatcggcc gcatgtccga ccgcggcttc gccgcctacg ggacggccaa
     1321 gggcgccctc acccacctca cccgcctcct cgcctctgat ctcgcccccc gggtccgggt
     1381 caacggcatc gccgtcggct cggtggccac ctccgccctc caggtcgtcc tcgacagcga
     1441 cgagatccac gacgagatgg tccgccgcac tcccctccag cgcctcggcg accccaccga
     1501 catcgccgcc gccgccctct ggctcgccag cccagccggc gactgggtca ccggcaaggt
     1561 cgtcgaagtc gacggcggcc tcgagtcacc gaacctggcc atgggcctgc cggacctcta
     1621 aggctgggcc aaccgatggc aaccaggggg cctggctcgt cgggctcacg tctgcggtgc
     1681 ccttcaggcc cgtttcgtca ccctccgtga ccgaatggca gcctggacgc tggcctcagc
     1741 gaactggcgc ctgattgggg tgctatggca cccgaatcag ccgccagaac gaggacctgt
     1801 cacggccagt gacattcgca ctgccgcgct gcttgcccgc ccccgatcgg tggcggcccg
     1861 tccccccgcc cccgtggttg cgccgttgtc ggttggccgg accctagagg tcggactcgt
     1921 caaccgagaa cgtgtcgcag gcttggacgt cgccggtctc gtagccggtg cggagccagc
     1981 ggacgcgttg ctcgctggag ccgtgggtga agctctcggg tgagtccacg ccgaggcggt
     2041 cgtcgccgac ggacgcggcg gcgtcgaggc cctcctcgat gtccccgtcg ctgaggaggt
     2101 cccgctggaa ggcggagtgg gcccagaccc cggcaaagca gtcggcctgc agctccaggc
     2161 gcacggacag ctcgttgctg ctcggcgggt cgatgcccag cacgttctgg acgtggtggc
     2221 ccagttcgtg ggccagcacg taggcctggg cgaagtcgcc gggcgccccg aagcggtcgg
     2281 tggtcagctc ccggaagaag gcgaggtcga tgtaggcctt ccggtcgacc gggcagtaga
     2341 agggcccggt ggcggcggag gcgggcccac agcccgactg cacgctgttc cggaacagga
     2401 ccagctcggc ctcggggtag ttctcgcctg cgtcgtcgaa gtagtcgtac cagaagccct
     2461 ggacgtcgtt gaggacgaag gacacgaagt ccacggtgtt ggcgtcaggg tcgtcggccg
     2521 ggtcgagcgg atcctcccct gccgcggccc cgcccgcggg cgagaactgg ctgaggatgt
     2581 cgttgatgtc ctcgacgtcg aagccgccag aggccgaatc gcctccgctc ccgtcggaca
     2641 cgcagctctt gcccagcagg acgaaggcca acaggacgac gatcccgatg accccgcccc
     2701 ccttgccgcc accggggatg aggccgccca agccgccgag tccccccgcc gagctgcccc
     2761 ggcggtcctc gatgttctcg ctccgaccct gacccggctt ccagcgcatg ggccctccct
     2821 accccgaatt gcgctcagtc gccttcgtcg ccgttcccgt ttccgttccc gttgccgttc
     2881 ccgtttccgt tcccattgcc gttgccgttg ccctcgccgt tgggaccgtc gggttccccg
     2941 tcgccctggc gggccagcag gacctggcag gcgtccacgt tgccgtcgtc gcagaaggcc
     3001 tgcagttgat cgtcgtcgag ctggtcaca
//
LOCUS       DASTFV010000192         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_731353, whole genome shotgun sequence.
ACCESSION   DASTFV010000192 DASTFV010000000
VERSION     DASTFV010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731353"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..631
                     /gene="leuD"
                     /locus_tag="VFP02_03630"
     CDS             35..631
                     /gene="leuD"
                     /locus_tag="VFP02_03630"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET9542146.1"
                     /translation="MEAVNVVTGTAVPLDRSDVDTDQIIPSDWLKRVERTGFGRGLFA
                     EWRDDRDFVLNDEQYAGASILVAGSNFGTGSSREHAVWAIQDYGFRAVISPRFADIFR
                     NNATKCGLVPVQVDAAVGEALMRAVEADPTLELTIDVERGTVAAPAVGIQAPFEMDEN
                     TRHRLVNGLDDIGLSLQHVTEIDGYETTRPAWLPSVRA"
     gene            699..2051
                     /locus_tag="VFP02_03635"
     CDS             699..2051
                     /locus_tag="VFP02_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9542147.1"
                     /translation="MAERVDVVVVGAGVAGLVAGRRLQEADRTVVVLEARDRVGGRTL
                     NDTIAGVTVEVGGQWVGPTQDRINALIDELGLERYPTYDEGQTVVELPGGKVKRFSGD
                     TPPFSPLALVDLLQAQRALDKLVETVDLDAPWDTPDAPALDAMTFASWLDRRLHTDQA
                     RAFHEILTAAALATEPDTMSLLHWLTYIQSGHGLERIIAVTGGAQQDRVVGGSWRVSE
                     RLAEDLGEAVVTSAPVRRIAAGDDAVTVTADTGSFEAAQVIVATAPNLAGRIDYEPAL
                     PARRDLLTQQLPHGHVIKVMVAYDEPWWRAEGLNGQAASVDGPVGIVFDNTVPGHDPG
                     ILVGFYEGSHALYWGERSPTARRQAFVDCLVRYFGPKAASPIDYVERDWAAEAWTRGC
                     YGAHFPTGAWTRYGPALREPCGPIHWAGTETATRWMGYIDGAVESAERSATEVLTALT
                     "
BASE COUNT          322 a          820 c          734 g          302 t
ORIGIN      
        1 gccacccccg acgacctcga ctaggagcgt cccgatggaa gccgtgaacg tggtgacggg
       61 gacggcggtc ccgctggacc ggtccgacgt ggacaccgac cagatcatcc ccagcgactg
      121 gctcaagcgc gtggagcgca ccggtttcgg tcggggtctc ttcgcggagt ggcgggacga
      181 ccgggacttc gtgctcaacg acgagcagta cgcgggagcg tcgatcctcg tggccggctc
      241 gaacttcggc accggctcct cccgcgagca cgccgtgtgg gccatccagg actacggctt
      301 ccgggccgtg atctcccccc gcttcgccga catcttccgc aacaacgcca ccaagtgcgg
      361 cctcgtgccc gtgcaggtgg acgcggccgt gggcgaggcc ctgatgcgcg cggtggaggc
      421 cgacccgacg ctggagctga ccatcgatgt cgagcggggc accgtcgccg ccccggcggt
      481 cggcatccag gcccccttcg agatggacga gaacacccgg caccgactgg tcaacggcct
      541 cgacgacatc ggcctgagcc tccagcacgt caccgagatc gacggctacg agaccacccg
      601 ccccgcctgg ctaccgtcag tccgtgcctg acctcaccgt tctggttgca tctccccgcg
      661 gtgagccgcg gcacggtgca accagaacgg ggtgagtcgt ggctgagcgc gtggatgtgg
      721 tggtcgtggg ggccggggtg gcgggcttgg tcgccgggcg acggctccag gaggccgatc
      781 ggaccgtcgt cgtgctcgag gccagggacc gggtcggcgg ccgcacgctg aacgacacca
      841 tcgccggggt caccgtcgag gtcggtggcc agtgggtcgg tccgacccag gaccggatca
      901 acgccctcat cgacgagctc ggcctcgagc ggtacccgac ctacgacgag ggccagacgg
      961 tcgtcgagct gcccggcggc aaggtcaaac gcttctccgg ggacacgccg ccgttcagcc
     1021 cgctggccct ggtcgacctc ctccaagccc agcgggccct ggacaagctg gtcgagaccg
     1081 tcgatctgga cgccccttgg gacacccccg acgcgccggc cctcgacgcc atgacgttcg
     1141 cctcctggct cgacaggcgg ctgcacacgg accaggcccg ggcgttccac gagatcctca
     1201 cggccgctgc cctcgccacc gagcccgaca ccatgtcgct gctccactgg ctgacctaca
     1261 tccagtcggg ccacggactg gagcggatca tcgccgtcac cggcggcgcc cagcaggacc
     1321 gggtggtcgg cggttcctgg cgggtcagcg agcgcttggc cgaggacctg ggcgaggccg
     1381 tcgtcacctc ggcacccgtt cgcaggatcg ccgccggcga cgacgccgtc accgtcacgg
     1441 ccgacaccgg ttcgttcgag gcggctcagg tgatcgtggc cacggccccg aacctggccg
     1501 gccgcatcga ctacgagccc gccctgcccg cccggcggga cttgctcacc cagcagctcc
     1561 cccacggcca cgtgatcaag gtcatggtcg cctacgacga gccgtggtgg cgggcggaag
     1621 gcctgaacgg ccaggccgcg tcggtcgacg gtcccgtcgg catcgtgttc gacaacaccg
     1681 tgcccggcca cgaccccggg atcctggtcg ggttctacga gggctcccac gccctctact
     1741 ggggcgagag gtcgcccacc gcccgccgcc aggcctttgt cgactgcctg gtccgctact
     1801 tcggcccgaa ggcggcgagc cccatcgact acgtcgagcg ggactgggcc gccgaggcct
     1861 ggacccgggg ctgctacggc gcccacttcc ccacgggggc ctggacccgc tacggccccg
     1921 ccctccggga gccctgcggc ccgatccact gggccggcac cgagaccgcc acccgctgga
     1981 tgggctacat cgacggtgcc gtcgagtccg ccgaacgctc cgccaccgag gtcctcaccg
     2041 ccctgacgta gccgggaacc gcagcctctc ccgcagcccg acgagcctca gtcagggccc
     2101 agtaacggtg tcccgcactt cagcacggtc ccggatccgg atcaccttgt tgatcgcatt
     2161 gagaagcgcc cgagccga
//
LOCUS       DASTFV010000193         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_732517, whole genome shotgun sequence.
ACCESSION   DASTFV010000193 DASTFV010000000
VERSION     DASTFV010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732517"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFP02_03640"
     CDS             complement(<1..266)
                     /locus_tag="VFP02_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542148.1"
                     /translation="MRRSTVERRLRDNSRRLQLLREELGITAEQMTQLGEEAEDARIR
                     AMVSETALAQREHSDAERHAAALSRHRAEVEAEIERLEQDQDRLL"
     gene            327..803
                     /locus_tag="VFP02_03645"
     CDS             327..803
                     /locus_tag="VFP02_03645"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HET9542149.1"
                     /translation="MRRLFLVLVLLAPLSACGGTDQADCGPVTREALDPNSGLHVLPD
                     QDQPRYTTDPPTSGAHYSLPPPTGVQDDPIERPMQVTALEGGQVLVQYRDLAAADVTR
                     LEDLASGVVVVAPNPDQAEPVVLTAWITKQTCSGVDVDTVATFIDEQAGHGPGTDF"
     gene            1044..1128
                     /locus_tag="VFP02_03650"
     tRNA            1044..1128
                     /locus_tag="VFP02_03650"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1079..1081,aa:Leu,seq:caa)
     gene            1405..>2502
                     /gene="fabD"
                     /locus_tag="VFP02_03655"
     CDS             1405..>2502
                     /gene="fabD"
                     /locus_tag="VFP02_03655"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET9542150.1"
                     /translation="MIAFTFPGQGSQKPGMGEPWKDHPSWELVEEATSVAGRDVAHLL
                     LEADADDLRETRNAQLATYVTSLVVLDAVERLGVDPARVAGHSLGEYTALTAAGVLSF
                     ADGIRLVTERGEAMQVAAAERAGTMAAVVALDDDDVAVACARTDGDVWLANHNAPGQV
                     VIAGAPEAIEAAGAHAKELGARRVIPLEVSGAFHTPFMASARDRLRKALAKADLRDAE
                     LPVLANVDAHAHTSAADWENLLAAQLCSPVRWRHILHALHDEGVSLFVELGPGAALTG
                     MAKRTVEGVTTLSVSVPDDLDKLLNTISEPRSADVAHEAGEHLFVTERLVVSPGAGVF
                     RSEPGVGAGTPVEAGTVVGHVGDLEVRSAFAG"
BASE COUNT          355 a          944 c          863 g          340 t
ORIGIN      
        1 agcagacggt cctggtcctg ctccaggcgc tcgatctcgg cctccacctc agcccggtgc
       61 cgggacaggg cggccgcgtg gcgctcggcg tcgctgtgct cgcgctgggc cagtgcggtc
      121 tccgagacca tggctcgaat gcgggcgtcc tcggcctcct cgccgagctg ggtcatctgc
      181 tcggcggtga tgcccagctc ctcgcggagc agctgcaggc ggcgggagtt gtcccggagc
      241 cgtcgctcca cggtcgaccg gcgcacggcc ctgatcctac gaggccgact ggcgggcgga
      301 ggggacgccc cgggcatcat ggcggcgtgc ggcgcctgtt cctggtcctg gtcctgctgg
      361 cgcccctgag cgcatgcggc ggcacggacc aggccgactg tggtccggtg accagggaag
      421 cactcgaccc gaactcgggt ctgcacgtcc tccccgacca ggaccagccg aggtacacga
      481 ccgacccgcc cacgtccggc gcccactact cgctgccgcc gcccaccggg gtccaggacg
      541 atccgatcga gcgcccgatg caggtgaccg ccctcgaggg cggtcaggtc ctcgtccagt
      601 accgggacct cgcggccgcc gacgtgaccc ggttggaaga cctggcgtcc ggcgtcgtgg
      661 tggtggctcc caaccccgac caggccgagc ccgtcgtgct gaccgcgtgg atcaccaagc
      721 agacctgctc cggcgtggac gtggacacgg tggcgacctt catcgacgaa caagcgggcc
      781 acggtcccgg gaccgacttc tgacggcctc ggcgatcacg acgaggatca cggccaggca
      841 gccgccgaag gggatccaca gcagggcgat gcccgccgtc gatgactcgt cgccgatgcc
      901 gacagccagg ggcgcccacc aacgggagca acagccacgg cgctcgacgt cccaccgaca
      961 gctcctccac ccccgggggt ttgtcccacc cccttcgtcg tttgaggagg gatggctcag
     1021 accaacctcg ttacgctgct ggcgcggcgg tggcgcaaca cggctgacgc gatgccctca
     1081 aaaggcattg tccgcaagga catgcgggtt cgaatcccgt ccgccgcaca acgacggcca
     1141 acggccagaa catcgcgggg tcatggcgac cggacgtgga gcacctcgac cgcttcatcg
     1201 ggccgaagtg gtagctacgg tgggcgggga tgaagtggct ggtcggggtc gtcgctgtgg
     1261 tggccctgat cgccgccgtc cgcgagcagc ggatccggtc gctcgaggcc cagttcgatc
     1321 cgggcccgac gccgtgacgg gcccggtgaa accatcctcg ccctccgccg gtctgaccac
     1381 cgtggcggca cccggtaggc cagcatgatc gcgttcacgt tcccggggca gggctcccag
     1441 aagcccggca tgggtgagcc ctggaaggac catccctcct gggagctggt cgaggaagcc
     1501 accagcgtcg ccggccggga cgtggcccat ctcctgctcg aggccgacgc cgacgatctc
     1561 cgcgagaccc gcaacgccca gctcgccacc tacgtcacca gcctggtcgt cctcgacgcc
     1621 gtcgagcgcc tcggtgtcga cccggcccgg gtcgccgggc acagcctggg tgagtacacg
     1681 gccctcacgg ccgccggcgt cctctccttc gccgacggca tccgcctggt caccgagcgg
     1741 ggcgaggcca tgcaggtggc cgcggccgag cgggccggga ccatggccgc cgtggtcgcc
     1801 ctcgacgacg acgacgtggc cgtggcctgc gcccgtaccg acggcgacgt ctggctggcc
     1861 aaccacaacg cccccggcca ggtcgtcatc gccggcgccc ccgaggccat cgaggccgcc
     1921 ggcgcccatg ccaaggagct cggcgcccgc cgggtcatcc ccctcgaggt cagcggggcg
     1981 ttccacacgc ccttcatggc gtcggcccgg gaccgcctgc gcaaggccct ggccaaggcc
     2041 gacctccgcg acgccgagct gcccgtgctg gccaacgtcg acgcccacgc ccacacctcc
     2101 gccgccgact gggagaacct gctcgccgcc cagctctgca gccccgtgcg ctggcgccac
     2161 atcctccatg cgctgcatga cgagggcgtc agcctgttcg tcgagctcgg tccgggagcg
     2221 gcgctgaccg gcatggccaa gcgcaccgtc gagggcgtga ccacgctcag cgtcagcgtc
     2281 cctgacgacc tcgacaagct gttgaacacg atctccgagc cccgctccgc cgacgtggcc
     2341 cacgaggccg gcgagcacct cttcgtgacc gaacgcctgg tggtcagccc gggggccggc
     2401 gtgttccgga gtgagcccgg ggtcggggcg ggcacaccgg tcgaggcggg caccgtggtg
     2461 ggtcacgtcg gtgacctcga ggtccggtcc gccttcgccg gc
//
LOCUS       DASTFV010000194         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_733229, whole genome shotgun sequence.
ACCESSION   DASTFV010000194 DASTFV010000000
VERSION     DASTFV010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733229"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..659
                     /locus_tag="VFP02_03660"
     CDS             159..659
                     /locus_tag="VFP02_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET9542151.1"
                     /translation="MTGPFDDLLEANQRNQGSFALGGLTGRPRLHLAVLACMDTRIDP
                     LAVLGLEPGDAKILRNAGARVTDDVVRSLAVVVANLGVNRVAVIAHTDCAMAKLTADE
                     LRARVRAASGTDAADDWDPLATPDQAEALRADLARLSSDPLLPDDLVVGAFIYDVATG
                     ALNPLD"
     gene            complement(643..1254)
                     /locus_tag="VFP02_03665"
     CDS             complement(643..1254)
                     /locus_tag="VFP02_03665"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9542152.1"
                     /translation="MDLPCEDPDATLDDYAQAVVDGAAGFDEPVIVVGHSLGGLTIPL
                     IPSRRPVAGLVFVAAILPAPGELAGAGLGRDAFSKGFPELTAAHQVAEENGASRWDRE
                     GAVAAFYHDVPEERLDAATDALRLQQWGPTREEWPLEAYPDVPMRYVACADDRILDPD
                     WQVRSARELLGIEADVLDSSHSPMLAQPAALADLLVAPFNPAG"
     gene            complement(1357..>2421)
                     /locus_tag="VFP02_03670"
     CDS             complement(1357..>2421)
                     /locus_tag="VFP02_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9542153.1"
                     /translation="ATATINYTSGTTARPKGVQLTHRNIWLNATTFGWQAGVSDRDVY
                     LHTLPMFHCNGWGMPYAHTGMGGTQVVIRKVDGAEILRRVEAHGVTQLNGAPAVVAAI
                     LDAAAEWDGPVPGAGRTRIVVAGAPPPTRTIERVETELGWEFVQIYGLTETTPLLTMN
                     RGRAEYDALSPAERAERLGRAGAPALGVQMRVDDEGELLARGNHVMDGYWVQPDATAD
                     AIVDGWFHTGDGGSIDDENYVAIADRKKDVIISGGENVSSIEVEDALFSHPAVAEVAV
                     IGVPDDKWGETVTALVVVTPGADASEAHLIAHCRERLAHYKCPTSIQFRDELARTATG
                     KLQKFKLRAPFWEGREKQVG"
BASE COUNT          321 a          898 c          856 g          346 t
ORIGIN      
        1 accggaccga cgccgtgcgc aaggcctaca tcgtccagta cgcccccgac ggcgcccaga
       61 ccttcgacga gcggggtcgg gtcgccgccc gctgcgacct ccccgaccgc cagttcctgg
      121 tgacgtaggc cgcgtccctc accgttaccg tcccgttcgt gacgggaccg ttcgacgatc
      181 tcctcgaagc caaccagcgg aaccaggggt ccttcgcctt gggcgggctc accggccggc
      241 cccggctcca cctcgccgtc ctggcctgca tggacacccg catcgacccc ctcgccgtgc
      301 tcggcctcga gccgggcgac gccaagatcc tgcgcaacgc cggcgcccgc gtgaccgacg
      361 atgtcgtgcg ctcgctggcc gtcgtcgtcg ccaacctggg cgtcaaccgg gtcgcggtga
      421 tcgcccacac ggactgcgcc atggcgaagc tcaccgccga cgagctccgg gcccgggtcc
      481 gcgccgccag cggcaccgac gccgccgacg actgggatcc gctggccacc cccgaccagg
      541 cggaggcact ccgcgccgac ctggcccgcc tgtcatcgga tcccctgctc cccgacgacc
      601 tggtcgtcgg cgccttcatc tacgacgtgg cgaccggcgc cctcaacccg ctggattgaa
      661 cggggcgacg agcaggtcgg ccagcgcggc cggttgggcc agcatcggcg agtgcgagct
      721 gtcgaggacg tccgcctcga tgccgaggag ctcccgagcg ctgcgcacct gccagtcggg
      781 gtcgaggatg cggtcgtcgg cgcaagccac gtagcgcatg ggcacgtccg ggtaggcctc
      841 gagcggccac tcctcccggg tcggtcccca ctgctggagg cgcagcgcgt ccgtggcggc
      901 gtcgagccgc tcctcgggga cgtcgtggta gaaggcggcc accgccccct cccggtccca
      961 ccggctggcc ccgttctcct cggccacctg gtgggccgcc gtcagctccg ggaacccctt
     1021 gctgaaggca tccctgccga ggcccgcccc cgccagctcg ccgggcgcag gcaggatggc
     1081 ggcgacgaag accagcccgg ccaccggtcg ccgggacggg atgagcggga tggtcagacc
     1141 gccgagtgag tgcccgacca cgatgaccgg ctcgtcgaag cccgccgcgc cgtcgaccac
     1201 cgcctgggcg tagtcatcga gggtggcgtc ggggtcctca cacgggaggt ccacggccac
     1261 cgccgactcg cccccgcgcc gtcagctcgt cgaccagggg ctgccagcag gcgccggcgt
     1321 gccacgcccc gtggacgagg acgaacgggg ccgtcgtcag cccacctgct tctcgcggcc
     1381 ctcccagaag ggggcgcgga gcttgaactt ctgcagtttg cccgtggccg tgcgggccag
     1441 ctcgtcccgg aactggatcg aggtcgggca cttgtagtgg gccagacgct cacggcagtg
     1501 ggcgatgagg tgggcctcag aggcgtcggc ccccggggtg acgaccacga gcgcggtgac
     1561 cgtctcaccc cacttgtcgt cggggacgcc gatgacggcg acctcggcga ccgcgggatg
     1621 ggagaagagc gcgtcttcca cctcgatgga cgacacgttc tcgccgccgg agatgatcac
     1681 gtccttcttg cggtcggcga tggccacgta gttctcgtcg tcgatggacc cgccgtcacc
     1741 ggtgtggaac cagccgtcga cgatggcgtc ggccgtggcg tcgggctgca cccagtagcc
     1801 gtccatcaca tggttgccac gcgcgaggag ctcaccttcg tcgtcgaccc gcatctggac
     1861 gcccaacgcc ggggcgcccg cccggcccaa gcgctcggcc cgctcggccg gggacagggc
     1921 gtcgtactcg gcccggcccc ggttcatggt gagcagcggc gtggtctcgg tcaggccgta
     1981 gatctggacg aactcccagc ccagctcggt ctccacccgt tcgatggtcc gggtcggtgg
     2041 tggggccccg gcgaccacga tgcgggtgcg ccccgctccg gggacgggac cgtcccactc
     2101 ggcggcggcg tcgaggatgg cggccacgac ggccggggcg ccgttgagct gggtgacccc
     2161 gtgggcctcg acgcggcgca ggatctcggc cccgtcgacc ttgcggatga cgacctgggt
     2221 accacccatg cccgtgtggg cgtagggcat gccccagccg ttgcagtgga acatcgggag
     2281 ggtgtgcagg tagacgtctc gatccgacac gccggcctgc caaccgaacg tggtggcgtt
     2341 gagccagatg ttgcggtgcg tcagctgcac gcccttcggc cgggccgtcg tgccgctggt
     2401 gtagttgatg gtggcggtgg c
//
LOCUS       DASTFV010000195         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_734925, whole genome shotgun sequence.
ACCESSION   DASTFV010000195 DASTFV010000000
VERSION     DASTFV010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734925"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..243
                     /locus_tag="VFP02_03675"
     CDS             43..243
                     /locus_tag="VFP02_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542154.1"
                     /translation="MILAFLLLAFAAVLAAGAAYEWHIKRHLPHDLREVGTEGLPSDA
                     SIRSGELGRGAASQYFRNLPPG"
     gene            complement(462..677)
                     /locus_tag="VFP02_03680"
     CDS             complement(462..677)
                     /locus_tag="VFP02_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542155.1"
                     /translation="MADDSSITSWAANRRRHRPPRIIGFKVRPYIPLRAARRAADATA
                     AAGVPPEAMTPTAANCDAPVNTSTDMA"
     gene            complement(687..995)
                     /locus_tag="VFP02_03685"
     CDS             complement(687..995)
                     /locus_tag="VFP02_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542156.1"
                     /translation="MDGEGGNDGQGGEEDGAPAGRRGMVTKAMAPPTMAAIRPGRRRR
                     WGAIRAMKPAGKAASSPNVAGSPMALPATAPIRVPRFQKTNTPTPVTQNAFSASVASR
                     "
     gene            1010..1324
                     /locus_tag="VFP02_03690"
     CDS             1010..1324
                     /locus_tag="VFP02_03690"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET9542157.1"
                     /translation="MSWTAILLLGALAYAFKVGGLLVLDHVKLPDSVATITALLPPAL
                     LAALVVVQTFADGTTLVLDARAAGLVAGIIAVALRAPFLVVVLSAASTTALLRAMTQT
                     LR"
     gene            complement(1309..2196)
                     /locus_tag="VFP02_03695"
     CDS             complement(1309..2196)
                     /locus_tag="VFP02_03695"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HET9542158.1"
                     /translation="MAIKVSYLSREVGINLKRNISLTVATIATVAVSLTLVGTAYLIS
                     RGVQDAQQQFQGGVEFIVFMNPNATDAQIEAVRTELDDNPQIREAEFCDQECAYAEFQ
                     ELFADDPQLLQNVEADILPPSFKVKPNNAEADAVDALGAVFETQPGVREVAFSSEAIK
                     RIESFYRFLSTRSAIAAVALVVAATLLVLNTIQMAILNRRRDIEVMKLVGATNWFIRI
                     PFMVEGLIQGLIGSLFAILVTRIFINDLEGQAEGAGLLSAFAVSGNQMTKASILLIAA
                     GAAVSLIGSGIAVSRYLKV"
     gene            complement(2204..2884)
                     /gene="ftsE"
                     /locus_tag="VFP02_03700"
     CDS             complement(2204..2884)
                     /gene="ftsE"
                     /locus_tag="VFP02_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET9542159.1"
                     /translation="MIKLENVTKHYMGGQVVALRDANAEIQKGEFVFLVGPSGSGKST
                     FIRLLNHEEVPDEGRIFVAGKDIVKLTPWKVPYLRRNIGCVFQDFKLLPNKTVYENVA
                     FALEVIGRPNNVIRSQVPAILELVGLSDKDKNMPNELSGGEQQRVSIARAFVNRPLIL
                     LADEPTGNLDPNTSVGIMRLLDRINRTGTTVVMCTHDRSIVDSMRRRVLQLDQGLIVR
                     DQARGVYE"
     gene            complement(3006..>3387)
                     /locus_tag="VFP02_03705"
     CDS             complement(3006..>3387)
                     /locus_tag="VFP02_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153759895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HET9542160.1"
                     /translation="SGAGGQHVNVTDSAVRITHLPTGIVTSCQNQRSQHQNRDKAMEI
                     LKARLADLRRKEHQDHLNELAGDQRSAEWANQIRSYFMHPEQRVKDHRSNYEMGNIQA
                     VLDGQVEPFMEAYLRWSRAEGEAS"
BASE COUNT          467 a         1170 c         1143 g          607 t
ORIGIN      
        1 catcgacgac ctcatcgaga ccgactacgc cgaaaacctc tgatgatcct ggccttcctg
       61 ctcctcgcct tcgccgccgt gctcgcggcc ggcgccgctt acgaatggca catcaagcgc
      121 cacctgcccc acgacctgcg cgaggtcggc acggaaggcc tgccctccga cgccagcatc
      181 cgctccggcg aactgggccg cggtgcggcc agccagtact tccgaaatct cccacccggc
      241 tagcggaacc ggggttcgtt cccctccgac agtcgatctc ctcgttaggt cacggtcgac
      301 catgatggcc gcccggccat gagcgacccg ccttctgttc ccgacccgag cgctcccccg
      361 ttccgtcggc gcgacggctt ggtcgtcgcc ctcgccgtcg gcgtgttcgg cgtgaccttc
      421 ggggtgttcg ccgtggccgc gggcctgagc gcaccacagg ctcaggccat gtccgtgctc
      481 gtgttcaccg gggcgtcgca gttcgcggcc gtcggcgtca tcgcctcggg cggcacgccg
      541 gccgccgctg tcgcgtcggc ggccctcctg gcggcccgca acgggatgta cggcctgacc
      601 ttgaacccga tgatccgggg tgggcggtgg cgtcgcctgt tggccgccca gctcgtgatc
      661 gacgagtcgt ccgccatggc gtccgctcag cgggatgcga cggaggcgga gaaggcgttc
      721 tgggtcacgg gcgtcggcgt gttcgtcttc tggaacctcg ggaccctgat cggcgccgtc
      781 gccggcaacg ccatcggtga cccggcgacg ttcgggctcg acgccgcctt ccccgccggc
      841 ttcatcgccc tgatggcccc ccacctccgg cggcgcccgg gcctgatcgc cgccatcgtc
      901 ggcggtgcca tcgccttcgt gaccatcccc ctcctcccgg ccggcgctcc atcctcctcg
      961 ccgccctggc cgtcgttccc gccttcgccg tccaacgccg cgagacggca tgagctggac
     1021 ggcgatcctg ctgctcggcg ccctcgccta cgccttcaag gtcggcggcc tgctcgtgct
     1081 ggaccacgtg aagctgcccg acagcgtcgc cacgatcacc gccttgctcc cgcccgctct
     1141 gctggctgcc ctcgtggtcg tccagacctt cgccgacggc accaccctcg tcctcgacgc
     1201 ccgcgccgcc ggcctggtcg ccggcatcat cgccgtcgcc cttcgggccc cgtttctcgt
     1261 cgtcgtcctc agcgccgcct caaccaccgc cttgttgcgg gcgatgactc agaccttgag
     1321 gtagcggctg acggcgatgc cggagccgat gaggctgacg gcggcaccgg cggcgatgag
     1381 caggatcgac gccttggtca tctggttgcc ggacacggcg aaggcggaca gcagtccggc
     1441 gccctcggcc tggccctcca ggtcgttgat gaagatccgg gtgaccagga tggcgaacag
     1501 ggacccgatg aggccctgga tcagaccctc caccatgaac ggtatgcgga tgaaccagtt
     1561 ggtggccccg accagcttca tgacctcgat gtcgcgacgg cggttgagga tcgccatctg
     1621 gatggtgttg agcaccagca gcgtggcggc cacgaccagg gccacggcgg cgatggccga
     1681 gcgggtcgag aggaaccggt agaagctctc gatgcgcttg atggcctcgc tggagaaggc
     1741 gacctcgcgc acgccgggct gggtctcgaa gacggcgccc aaggcgtcga cggcgtcggc
     1801 ctcggcgttg ttcggcttca ccttgaacga cggcggcaag atgtccgcct cgacgttctg
     1861 caggagctgc ggatcgtcgg cgaagagctc ctggaactcc gcgtaggcgc actcttggtc
     1921 gcagaactcc gcctcgcgga tctgggggtt gtcgtccagc tccgtgcgca cggcctcgat
     1981 ctgcgcgtcg gtggcgttgg ggttcatgaa gacgatgaac tccacgcccc cttggaactg
     2041 ctgctgggcg tcctgcacgc cgcggctgat caggtaggcg gtgccgacca gggtgagcga
     2101 caccgccacc gtggcgatcg tcgcgacggt gaggctgatg ttgcgcttga ggttgatgcc
     2161 gacctcgcgc gagagataac tgacctttat cgccatgggc cggttactcg tagacgccgc
     2221 gggcctggtc gcgaacgatc agcccctggt ccagctggag cacgcggcgg cgcatgctgt
     2281 cgacgatcga acggtcgtgg gtgcacatga ccacggtggt gcccgtgcgg ttgatgcggt
     2341 cgaggagccg catgatcccc accgaggtgt tgggatcgag gttccccgtc ggctcgtcgg
     2401 ccagcaggat cagcggccgg ttgacgaagg cccgggcgat ggagacccgc tgttgctcgc
     2461 cacccgacag ctcgttgggc atgttcttgt ccttgtcgga caggcccacc agctccagga
     2521 tcgccgggac ctggctgcgg atgacgttgt tggggcgccc gatcacctcg agggcgaagg
     2581 ccacgttctc gtacaccgtc ttgttgggca ggagcttgaa gtcctggaag acgcacccga
     2641 tgttgcgccg caggtagggc accttccacg gggtgagctt cacgatgtcc ttgcccgcca
     2701 cgaagatgcg gccctcgtcg gggacctcct cgtggttgag caggcggatg aaggtcgact
     2761 tgccggaccc ggacgggccg acgaggaaga cgaactcgcc cttctggatc tcggcgttgg
     2821 cgtcgcgaag cgcgacgacc tgcccgccca tgtagtgctt ggtgacgttc tcgagtttga
     2881 tcatgtgggg ggagagcgat gccgtggggt cgggtgtcgg cgaataccgc agtcccgccc
     2941 gaggccgcgt actcgacccg ggaatgtacc agtggccccg gtcagaaccg cgggatgccc
     3001 tgcggtcacg aagcctcgcc ctcggcccgg gaccagcgca ggtaggcctc catgaacggc
     3061 tccacctgac cgtccaggac ggcctggatg ttgcccatct cgtagttgct gcggtgatcc
     3121 ttcacccgct gttcggggtg catgaagtac gaccggatct gattggccca ctcggcgctg
     3181 cgctggtctc cggcgagttc gttcagatgg tcctgatgct ccttgcgccg aaggtcggcc
     3241 aggcgggcct tcaggatctc catcgccttg tcccggttct ggtgctggct gcgctggttc
     3301 tgacaggacg tcacgatccc ggtcggcagg tgggtgatgc gcaccgcgga gtcggtgacg
     3361 ttgacgtgct ggccaccggc gcccgac
//
LOCUS       DASTFV010000196         3106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_737679, whole genome shotgun sequence.
ACCESSION   DASTFV010000196 DASTFV010000000
VERSION     DASTFV010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737679"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..517)
                     /locus_tag="VFP02_03710"
     CDS             complement(155..517)
                     /locus_tag="VFP02_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542161.1"
                     /translation="MAVTLQGLRTLIHPVEDLEAAKAWWTDFLGFGPYFDEPFYVGYE
                     VAGYELGLLPGHPVTDGALPYWGVDDVAEAMAAAIASGATEHTPATEVGEGIVTGTVR
                     NPQGSIVGFIRNPHFTLP"
     gene            complement(542..1102)
                     /locus_tag="VFP02_03715"
     CDS             complement(542..1102)
                     /locus_tag="VFP02_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9542162.1"
                     /translation="MADDRSFAADLAGYRPASPEEAADLERARALLAGPDPWSRSVPL
                     HLTGSALVVHPPSARVLLRWHERQEAWLQVGGHADPGEVDPFVTALREGAEETGLEDL
                     RPWPGPEPTLVHLIAVPVPAGKGEPAHEHLDVRYVLATDTPAAARPENPSARLQWLTV
                     TEALAFVAEDNLRVALGRVADLLVDA"
     gene            complement(1109..1615)
                     /locus_tag="VFP02_03720"
     CDS             complement(1109..1615)
                     /locus_tag="VFP02_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9542163.1"
                     /translation="MAVRAPLKAELPLLQAIGVAAGRRFAEVGLDAVAEDPPPELSAL
                     ERWRAAGRAWVTTDRDGHPVGFVVVDLVDGTAHIEEISVLPERNGRGHGMALMRHVET
                     WARGKGFPAVTLTTFSEVPWNRPWYERRGYRVLEEPEWTPGLAQRRAEEAEAGLDPEL
                     RVIMAKAL"
     gene            complement(1618..2685)
                     /locus_tag="VFP02_03725"
     CDS             complement(1618..2685)
                     /locus_tag="VFP02_03725"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET9542164.1"
                     /translation="MTELQERWDRLAYWIGPYLGAEGAVTITDAGVPDGIGYSAETLL
                     FTANFPAAGGDCSRRLVLRGETADPAVYPAQVEDIDVEIEIQHRIMSTLAATTDIPVA
                     PIVGADLTDEAIGAPFFVMDYVDGVVPAVDPPYPAAGFFADADPADRGRMIADGVRAM
                     GQIHAVDWRAAGLGWLVPSGVEPTLRRQLDLWAAYARRELRGRVHPDMDRALAILEDR
                     LDEPSPPALCWGDPRPGNIIWQDFACASVTDWEAAAIAPPELDLGWWLMFDRSCHEVG
                     GFDRLEGEPSRDEQRDLYAEAVGRDVGDTALYELLAAYRYSAIVVRVLNRWVERGDFP
                     ADHDIWLENPAAACLHQLLES"
     gene            2733..>3106
                     /locus_tag="VFP02_03730"
     CDS             2733..>3106
                     /locus_tag="VFP02_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542165.1"
                     /translation="MPVVVAPTGLPVESAVADVRDALTTVGTAVLQAEPGAGKTTVVP
                     LRLLDEPWRGDGRIVVLEPRRLATRAAARRMADLLGERVGETVGYRTRDERHVGPATR
                     IEVVTEGILTRRLQADPSLPGTA"
BASE COUNT          427 a         1136 c         1149 g          394 t
ORIGIN      
        1 gacgagccca tgcgcttccg ggacttcatc gccaacagcc gccagtgcaa gctctacgac
       61 tacgacaccg gcacctggct gacctacccg gaagccgccg cccacctggc ggccaacccg
      121 gtcgagccgg agcccgccgc cacctgatct ctggtcaggg cagcgtgaag tggggattgc
      181 ggatgaaccc cacgatgctg ccctgggggt tgcgcaccgt gccggtgacg atcccctcgc
      241 ccacctcggt ggccggcgtg tgctcggtcg cccctgaggc gatcgccgcc gccatcgcct
      301 cggccacgtc gtcgaccccc cagtagggca gcgccccgtc ggtgacgggg tggccaggca
      361 gcaggcccag ctcgtagccc gccacctcgt agccgacgta gaacggctcg tcgaagtacg
      421 gcccgaagcc gaggaagtcg gtccaccagg ccttcgcggc ctcgaggtcc tcgaccgggt
      481 ggatgagcgt gcgcaatccc tgcagggtga cggccatgcc cccagccaac cacgcggctc
      541 gtcaggcgtc cacgaggaga tcggccaccc gcccgagcgc cacgcggagg ttgtcctcgg
      601 cgacgaaggc cagagcctcg gtcacggtca gccactgcag gcgggcgctc gggttctcgg
      661 gccgggccgc cgcgggcgtg tcggtggcca ggacgtagcg cacgtccagg tgctcgtggg
      721 cgggttcccc cttgccggcc ggtacgggga cggcgatgag gtggaccagg gtcggttcgg
      781 gaccgggcca cggccggagg tcctcgaggc cggtctcctc cgccccctcc cgtagggcgg
      841 tcacgaacgg gtcgacctcg ccggggtcgg cgtgaccgcc gacctgcagc caggcctcct
      901 ggcgctcgtg ccaccgcaac agcacccgcg cgctcggcgg gtggacgacg agggccgagc
      961 ccgtcaagtg caggggcacc gaccggctcc acgggtcggg gccggcgagc agggcgcggg
     1021 ctcgctccag atcggcggcc tcctccgggg atgccggccg gtagccggcg agatcggcgg
     1081 cgaagctgcg gtcgtcggcc acggcgcctc agagggcctt ggccatgatc acccgcagct
     1141 cggggtcgag accggcctcg gcctcctccg ctcggcgctg ggccaggccc ggggtccact
     1201 ccggctcctc cagcacgcgg tagccccggc gctcgtacca gggccggttc caggggacct
     1261 ccgagaacgt cgtgagggtg acggccggga agcccttgcc ccgggcccac gtctcgacgt
     1321 gacgcatcag ggccatgccg tggccccgcc cgttgcgctc cggcaggacc gagatctcct
     1381 cgatgtgggc ggtcccgtcg accaggtcca cgacgacgaa gccgacgggg tggccatcgc
     1441 ggtcggtggt gacccacgcc cgccccgcgg cccgccagcg ttcgagagcc gacagctcgg
     1501 ggggcgggtc ctcggccacg gcatcgaggc cgacctcggc gaagcggcgg ccggcggcca
     1561 ccccgatggc ctgcaggagc ggcagctcgg ccttgagggg cgcccggacc gccatgctca
     1621 gctctccagc agctggtgca ggcaggcggc cgccgggttc tccaaccaga tgtcgtggtc
     1681 ggcggggaag tccccccgct cgacccaacg gttcaggacg cgcacgacga tggccgagta
     1741 gcggtaggcg gcgagcagct cgtagagcgc cgtgtccccg acgtcccggc cgacggcctc
     1801 ggcatagagg tcccgttgct cgtctcggga gggctcgccc tccagacggt cgaacccccc
     1861 gacctcgtga caggagcggt cgaacatcag ccaccagccc aggtccaact cgggcggggc
     1921 gatcgccgcc gcctcccagt cggtgacgct ggcgcaggcg aagtcctgcc agatgatgtt
     1981 gccggggcga gggtcccccc agcagagggc gggtggcgac ggctcgtcga gtcggtcctc
     2041 gaggatggcg agggcgcggt ccatgtcggg atggacccgg ccccgcagct cacgccgggc
     2101 gtaggccgcc cacaggtcga gctggcggcg cagggtcggc tcgaccccgc tcggcaccag
     2161 ccagccgagc ccggcggctc gccagtccac ggcatggatc tggcccatgg cccgcacccc
     2221 gtcggcgatc atccggcctc ggtccgccgg gtcggcgtcg gcgaagaacc cggcggccgg
     2281 gtacggcggg tcgacggcgg gaaccacgcc gtcgacgtag tccatcacga agaacggggc
     2341 gccgatggcc tcgtcggtca ggtcggcgcc gacgatgggg gccaccggga tgtccgtcgt
     2401 cgccgccaac gtggacatga tgcggtgctg gatctcgatc tcgacgtcga tgtcctcgac
     2461 ctgggcgggg tagacggcgg ggtcggcggt ctcaccccgc agcacgaggc gccgggagca
     2521 gtcgccaccg gccgcgggga agttggcggt gaagagcagc gtctcggccg agtacccgat
     2581 gccgtcgggg acgccggcgt cggtgatcgt gaccgcgccc tcggccccga gatagggccc
     2641 gatccaatag gccaggcggt cccaccgctc ctgcagttcg gtcatcgtcc cgcgaagggt
     2701 agggggcgac tcgggaacgg cagactgcac ccgtgcccgt tgtcgtcgcc ccgaccgggc
     2761 tgcccgtgga gtcggcggtg gccgacgtcc gggacgcgct gaccaccgtg ggcaccgcgg
     2821 tgctccaggc cgagcccgga gcgggcaaga ccactgtggt gccgttgcgc ctgctcgacg
     2881 agccgtggcg cggtgacggg cgcatcgtgg tcctcgagcc tcgacgcctg gccacccggg
     2941 ccgcggcgcg acgcatggcc gatctcctgg gcgagcgcgt cggggagacc gtcggctacc
     3001 gcacccggga cgaacgtcac gtcggaccgg cgacccggat cgaggtcgtg accgagggga
     3061 tcctcacccg tcggctccag gccgacccga gcctgcccgg caccgc
//
LOCUS       DASTFV010000197         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_741492, whole genome shotgun sequence.
ACCESSION   DASTFV010000197 DASTFV010000000
VERSION     DASTFV010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741492"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VFP02_03735"
     CDS             <1..1099
                     /locus_tag="VFP02_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HET9542166.1"
                     /translation="TCDNHCEFCFIHQLPPGMRSSLYQRDDDYRLSFLYGNFTTLTRF
                     TEMDLERVITERLSPLYDSIHATDPDVRSEMLRNRRGATSLRWLRALLDAGVLVHGQV
                     VVCPGVNDGAVLEDTMVGIADQYPELATVAVVPLGISRHGREGRLRPHTVAEAEATVD
                     SIEAWQGRFLEIVGHRLVYAADEYYLLADRPFPVGESYEGFPLHEDGIGMARSFEQEF
                     AGEVDTPIGPRAGFFQSIDGAPAAGYRAPRGQVTLRPRRSAPVAVLTGTYGAEILAPL
                     LVDRDDVRVLAVENRFFGGNIGVTGLLVGEDLQRVLADQPPGHRYLLPDVCLSGGRFL
                     DGTTPDDLPLPVEVVATDGLSLRAALEPVAA"
     gene            1096..>2290
                     /gene="der"
                     /locus_tag="VFP02_03740"
     CDS             1096..>2290
                     /gene="der"
                     /locus_tag="VFP02_03740"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET9542167.1"
                     /translation="MSAPVVAVVGRPNVGKSTLLNRIVGRREAIVEERPGVTRDRKAV
                     DAEWRGRPFIVMDTGGFIATEEGLDAEVSRQSVRAIEDADAVVMVVDVTVGVTNEDEA
                     VASLLRGRDIPVFVVANKVDDTNRENDAWAFLSLGLGDPHPISALHGRRTGDFLDVLV
                     DALPPEGEDADGDEGEVREEILAVAIVGRPNVGKSTLFNTLDRAERSVVHDLAGTTRD
                     TIDTVIETESGPVRILDTAGMRRRGKIDVSTEYYSVVRALRAIDTADVVLLVIDALIG
                     VTHQDQRLAERASISGSPVVVLLNKWEMADADQRELVSEQVDDRLKFLPNAKVLKISA
                     LTGKGTHKIIDALLDTADTYHRRIPTAKVNEVLRQAQAHQPAPGGTRVLYATQGATDP
                     PTFTLF"
BASE COUNT          338 a          849 c          779 g          324 t
ORIGIN      
        1 cacctgtgac aaccactgtg agttctgttt catccaccag ctcccgccgg ggatgcgctc
       61 cagcctctac cagcgcgacg acgactaccg cctgtccttc ctctacggga acttcaccac
      121 gctgacccgg ttcaccgaga tggacctcga gcgggtgatc accgagcgcc tgtcgccgct
      181 ctacgactcc atacacgcca ccgatccgga cgtgcggtcc gagatgctgc gcaaccggcg
      241 cggcgccacc agcctgcgct ggctgcgggc cctcctcgac gccggcgtgc tggtccacgg
      301 ccaggtcgtg gtctgccccg gggtcaacga cggcgccgtc ctcgaggaca cgatggtcgg
      361 gatcgccgac cagtaccccg agctggcgac cgtcgccgtg gtgcccctcg gcatcagccg
      421 tcacgggcgc gagggacgtc tgcggcccca caccgtcgcg gaggccgaag ccaccgtcga
      481 cagcatcgag gcctggcagg gccgcttcct ggagatcgtg ggccaccggc tggtctacgc
      541 cgccgacgag tactacctgc tggcggaccg cccgttcccg gtgggggaga gctatgaggg
      601 ctttcccctc cacgaggacg gcatcggcat ggcccgcagc ttcgagcagg agttcgcggg
      661 cgaggtcgac accccgatcg ggccccgggc cggcttcttc cagtccatcg acggggcccc
      721 cgcggcgggc taccgggccc cccggggcca ggtcacgctc cgcccccggc gcagcgcccc
      781 cgtggccgtc ctcaccggga cctacggggc cgagatcctg gcccccctcc tggtcgaccg
      841 ggacgacgtg cgggtcctgg cggtcgagaa ccgcttcttc ggcgggaaca tcggggtcac
      901 cggcctgctg gtgggggagg acctgcagcg ggtcctggcc gaccagcccc ccgggcaccg
      961 gtacctcctc cccgacgtct gcctgtcggg tgggcgcttc ctggacggca ccacacccga
     1021 cgacctgccc ctgcccgtcg aggtcgtcgc caccgacggc ctgtcgctgc gggccgcgct
     1081 cgagccggtc gccgcatgag cgcgcccgtg gtcgccgtgg tgggtcgacc gaacgtgggc
     1141 aagtcgaccc tgctgaaccg catcgtgggc cgccgcgagg ccatcgtcga ggagcggccg
     1201 ggcgtcaccc gggaccgcaa ggccgttgac gccgagtggc ggggtcgtcc cttcatcgtc
     1261 atggacaccg gcggcttcat cgccaccgag gagggcctcg acgccgaggt cagccgccag
     1321 agcgtgcggg ccatcgagga cgcggacgcc gtggtcatgg tcgtcgacgt caccgtcggg
     1381 gtgaccaacg aggacgaggc tgtggcctcc ctcctgcggg ggcgggacat ccccgtgttc
     1441 gtggtggcca acaaggtcga cgacacgaat cgggagaacg acgcctgggc cttcctgtcc
     1501 ctcggcctcg gcgacccgca tccgatcagt gctctgcacg gccggcgcac cggagacttc
     1561 ctcgacgtgc tcgtcgacgc cctgcccccc gagggggagg acgccgacgg cgacgagggc
     1621 gaggtccggg aggagatcct ggcggtggcc atcgtgggcc ggcccaatgt gggcaagtcc
     1681 acgctgttca acacgctgga ccgggccgag cgttcggtgg tccacgacct ggccggcacc
     1741 acccgcgaca ccatcgacac ggtcatcgag accgagtccg gtccggtgcg aatcctggac
     1801 acggccggca tgcgccgtcg gggcaagatc gacgtgtcga cggagtacta ctccgtggtc
     1861 cgggccctac gggcgatcga caccgccgac gtggtcctgc tcgtgatcga cgcgctgatc
     1921 ggcgtcaccc accaggacca gcgcctggcg gagcgggcgt ccatctccgg gtccccggtg
     1981 gtcgtgctcc tcaacaagtg ggagatggcc gatgccgatc agcgggagct ggtgtccgag
     2041 caggtcgacg accggctgaa gttcctgccc aacgccaagg tcctcaagat cagtgccctc
     2101 acgggcaaag gcacccacaa gatcatcgat gccctgctgg acaccgccga cacctatcac
     2161 cggcgcatcc cgacggcgaa ggtcaacgag gtgctccgcc aggcgcaggc ccaccagccg
     2221 gcccccggag gcacccgggt cctctacgcc acccaggggg ccaccgaccc gccgacgttc
     2281 accctgttcg
//
LOCUS       DASTFV010000198         3175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_753710, whole genome shotgun sequence.
ACCESSION   DASTFV010000198 DASTFV010000000
VERSION     DASTFV010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753710"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VFP02_03745"
     CDS             complement(<1..574)
                     /locus_tag="VFP02_03745"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9542168.1"
                     /translation="MRIVILGAGFGGLELTTRLSEEFGDEVDVVLIDQSDGFVFGFSK
                     LDVMFGKVPAESVHHSYADLVKPGVTFVQSTVTSIDPGAKRVETDAGPFDADILVVAL
                     GADLHPDATPGLVEGGHEFYTVAGAFGLRDVLADFGGGRVIVAVTSTPFKCPPAPSET
                     ALLMHDHLVERGQREQSEIALVMPLGAPVPP"
     gene            complement(630..1052)
                     /locus_tag="VFP02_03750"
     CDS             complement(630..1052)
                     /locus_tag="VFP02_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542169.1"
                     /translation="MDVLAALVEHGMLLQSAKGQVPNVADLVAGESIKGSWWGHPAGH
                     QIFATLNELDESPDVIRLRLVKGKVTLVHRRLWPAVVCLADRFPPERLAALHEEHTES
                     GRHRVEEQPFPDWVPADVLAEAGRLSADDAEALLAPAL"
     gene            complement(1055..1717)
                     /locus_tag="VFP02_03755"
     CDS             complement(1055..1717)
                     /locus_tag="VFP02_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HET9542170.1"
                     /translation="MPGQRERARQAVLAEITAEGRRQLARDGAAALSLRSVARELDMV
                     SSGIYRYVESRDALLTLLIIAGYDRLGSALERAGADEGTPRERWRATCGALRAWARAN
                     PHEYALLYGSPVPGYAAPQDTIDPATRVYAALAAPLRDAPAPADRPAVSGALATDGER
                     IADGLGLALPPAQGVALLGAWATLFGLVSLELFGHTHNVVTDHDAFFAHHVDALAADL
                     GL"
     gene            1828..2295
                     /locus_tag="VFP02_03760"
     CDS             1828..2295
                     /locus_tag="VFP02_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HET9542171.1"
                     /translation="MTTSPGTTTHRYVEPGKGDHRMNRVVGWLVRHGISVAGARVLAV
                     RGRTSGEERTTVVNLLELDGERYLVAPRGRTQWVRNLRAAGTGDLRVGRRTQAFRADE
                     LADADKGPVLRAYLDRWGWEVGRFFEDLAKDSDDEAVAAVAPDFPVFRVVAAS"
     gene            complement(2296..2547)
                     /locus_tag="VFP02_03765"
     CDS             complement(2296..2547)
                     /locus_tag="VFP02_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET9542172.1"
                     /translation="MRVWIDQDLCTGDGLCVDHCPEVFVLLEDGISYVMADGRPQNDP
                     GGAESMVMVPPVLDRPVIEAAEDCPGECIFVELDAPANA"
     gene            2607..2855
                     /locus_tag="VFP02_03770"
     CDS             2607..2855
                     /locus_tag="VFP02_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542173.1"
                     /translation="MSDCADQYPPSDETRWFVQVIDTAAVDEGTVDSLTLNGPDGESI
                     EFDDLPATIPDDDPQGLVLLTDGDRAESRRLPGAGGHA"
     gene            2842..3102
                     /locus_tag="VFP02_03775"
     CDS             2842..3102
                     /locus_tag="VFP02_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542174.1"
                     /translation="MATPDSSNSEAGLQIDQPLESCAAYFPPGADIRLYLYVADTPAQ
                     DTGQLVDATITGPDGETYAVEDIGMTIPDADPDGVVSFFAPA"
BASE COUNT          490 a         1108 c         1147 g          430 t
ORIGIN      
        1 acggtgggac gggggcgccg aggggcatga cgagggcgat ctcggactgc tcccgctgcc
       61 cgcgctccac caagtgatcg tgcatgagca gggcggtctc gctgggggcc ggagggcact
      121 tgaacggcgt ggaggtgacg gccacgatca cccgcccgcc cccgaagtcg gccaggacgt
      181 cgcggagacc gaaggcgccg gcgaccgtgt agaactcgtg gccaccttcg accagccccg
      241 gcgtggcgtc cgggtgcagg tcggcgccga gggccaccac caggatgtcg gcgtcgaagg
      301 gcccggcatc ggtctcgacc cgcttggcgc ccgggtcgat cgaggtcacc gtcgactgga
      361 cgaaggtcac gccgggcttg acgagatcgg cgtaggagtg atgcaccgac tcggcgggga
      421 ccttgccgaa catgacgtcc aacttggaga acccgaacac gaacccatcg ctctggtcga
      481 tgagcacgac gtcgacctcg tcgccgaact cctcggacaa ccgggtcgtc agctccaggc
      541 cgccgaagcc ggccccgagg atcacgatgc gcacggacga aagctagccc gacgacatcg
      601 acccctccgg cgggccgccc gggcgaatct cagagcgccg gcgccaggag ggcctcggcg
      661 tcgtcggcgc tgaggcgccc ggcctcggcc agcacgtcgg cggggaccca gtccggaaaa
      721 ggctgttcct cgacccgatg ccggccggac tcggtgtgct cctcgtggag cgcggccagg
      781 cgctcgggtg ggaagcggtc ggccaggcac acgacggccg gccacagccg gcggtgcacg
      841 agggtgacct tgcccttcac caggcggagg cggatcacgt ctggcgactc gtccagctcg
      901 ttcagggtgg cgaagatctg gtggccggcg ggatggcccc accagctgcc cttgatcgac
      961 tcgcccgcca ccaggtcggc gacgttgggg acctgtcctt tggccgactg caggagcatc
     1021 ccgtgttcga ccagggccgc caggacgtcc acggtcacag accgaggtcg gcggccaagg
     1081 cgtcgacgtg gtgggcgaag aaggcgtcgt ggtccgtcac cacgttgtgg gtgtggccga
     1141 agagctccag ggagaccagc ccgaacaggg tggcccaggc tccgaggagg gccacgcctt
     1201 gcgccggggg gagtgccaag ccgagaccgt cggcgatccg ctcgccgtcg gtggccagcg
     1261 cgcccgacac cgccggacgg tccgccgggg ccggcgcgtc ccgcagtggc gccgccaggg
     1321 cggcgtacac ccgggtggcg gggtcgatcg tgtcctgtgg cgcggcgtag ccgggcaccg
     1381 gcgacccgta cagaagggcg tactcgtgcg gattggccct ggcccaggcc cgcagggcgc
     1441 cgcaggtcgc ccgccagcgc tcccgagggg tcccctcgtc ggcgccggcc cgctcgaggg
     1501 cggagcccaa ccggtcgtag ccggcgatga tcagcagggt gagcagcgcg tcacggctct
     1561 cgacgtagcg gtagatgccc gaggacacca tgtccagctc gcgggccacc gagcggaggg
     1621 acagggcggc ggccccgtcc cgggccagct gacggcggcc ctcggcggtg atctcggcga
     1681 gcacggcctg gcgggctcgt tcacgttgtc cgggcatggg aacagcatgc cacagaaatg
     1741 tgagcagtgc tcttgacagt gagcgttgct cgccatatag tgagcactgc tcacacaacg
     1801 ggagcatcgc tctcaaggag accaccgatg accacgagcc ctggcaccac cacccaccgc
     1861 tacgtcgagc ccggcaaggg cgatcaccgc atgaaccggg tggtcggctg gctcgtccgc
     1921 cacgggatca gcgtggccgg cgcccgcgtc ctggcggtcc ggggtcggac ctcgggtgag
     1981 gagcggacca cggtggtcaa cctcctcgag ctcgacggcg agcgctacct ggtcgccccc
     2041 cggggccgga cccagtgggt ccggaacctg cgggccgccg gcaccggcga cctgcgggtc
     2101 gggcggcgga cgcaggcctt ccgggccgac gagctggccg acgccgacaa gggcccggtc
     2161 ctgcgggcct atctcgaccg ttggggctgg gaggtcggtc gcttcttcga ggacctggcc
     2221 aaggacagcg acgacgaggc cgtcgccgcc gtcgcccccg acttcccggt cttccgggtg
     2281 gtggccgcgt cctgatcagg cgttggcggg tgcatccagc tcgacgaaga tgcactcgcc
     2341 ggggcagtcc tcggccgctt cgatcacggg gcggtcgagg accggaggga ccatgaccat
     2401 gctctcggcc ccaccggggt cgttctgggg ccggccgtcg gccatgacgt aggagatccc
     2461 gtcctcgagg agcacgaaca cctcggggca gtggtcgacg cacaacccgt caccggtgca
     2521 gaggtcctga tcgatccaca cccgcatgag ctcctcctcg atgacgatgt taggccggtg
     2581 acggtggcct gtcgggcagc atcgacatgt ccgactgtgc cgaccagtac ccgccgtcgg
     2641 acgagacccg gtggttcgtc caggtgatcg acaccgcggc cgtcgacgaa gggaccgtcg
     2701 actccttgac cctcaacggg ccggacggcg agtccatcga gttcgacgac ctgccggcca
     2761 ccatccccga cgacgacccg caggggctgg tcctgctgac cgacggggac cgggccgagt
     2821 cacgtcgtct gccaggagca ggtggccacg cctgactcgt ccaacagcga ggccggcctc
     2881 cagatcgacc agcccctgga gagctgtgcg gcctacttcc ctcccggcgc cgacatccgc
     2941 ctctacctgt atgtcgccga caccccggct caggacaccg gtcagctggt cgacgccacg
     3001 atcaccggac cggacggcga gacctacgcg gtcgaggaca tcgggatgac gatccccgac
     3061 gccgaccctg acggggtggt cagcttcttc gctcccgcct gaggtcagcc ggcggcgtcg
     3121 aagtgcttgc ggaagcgctg gccgacggcc atgttctggc ccatgcggtc ggtcc
//
LOCUS       DASTFV010000199         2958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_760168, whole genome shotgun sequence.
ACCESSION   DASTFV010000199 DASTFV010000000
VERSION     DASTFV010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760168"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFP02_03780"
     CDS             <1..384
                     /locus_tag="VFP02_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542175.1"
                     /translation="RADIRAFYGEVFDFEALDVPILGQLGLLLRTDEATSQFLLLMES
                     ETPIDAPGYDHLGFLYDTREQVDDRLEQVKRWQEKDERVRIKEYEDLVTGATTVHAFY
                     VRYLLPIWFDVQSIEHAGPIPSRED"
     gene            386..1606
                     /locus_tag="VFP02_03785"
     CDS             386..1606
                     /locus_tag="VFP02_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9542176.1"
                     /translation="MAIDFTLTPELEELRTKVRTFLDEVVVPTEKTLDPDDRSAYLTA
                     LLGMRKAAFEAGLWCPHMPEEWGGMGLSHVEMAMVQAEAAKTYYGPWVLNCQAPDEGN
                     MHTLLHWATPEQAEKYLRPLCDGKAWSCFAMTEPEVAGSDPTLIQTRAYQDGDEWVIN
                     GHKWFISNAHRANFAILIARTEDDPDLPQAANSAFIVDIPSDGWTEVRQVETMHGSTG
                     HSEILIEDLRVHDDQMLGGRGEGHLLGQSRLGPARLAHCMRWIAQAETALDMMVDRSL
                     NRFSHGSILAEKQGVQWMIADSAMELYQAKLMVLHAAYKIDRGDDFKSEVSMAKHFVA
                     NALNRIIDRSIQVHGALGYSTDTPLAGMFQHARWARFADGADEIHQMRIAQRTIAAYK
                     DFGTTKAATGDLPI"
     gene            complement(1613..2470)
                     /locus_tag="VFP02_03790"
     CDS             complement(1613..2470)
                     /locus_tag="VFP02_03790"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542177.1"
                     /translation="MAAKARRLELEVDGRELSISNPDKVLFPDDGITKAEMVQYYERI
                     APLLVRHAQGRPVALHRFPDGIGKGGFFQKQVGKHFPDWIPRVELTTRDGSTEFVVIE
                     DAATVVYLAGQGVLVLHTLFSRADDPYRPVEVMVDLDPSTPDRGPVRHAAGRLRELFG
                     DRGYTARVKTSGSRGLHVVVDVDLPDFPAARGLALEVSEQLVAEEPDEYTLEFYKEKR
                     GDRLLLDINRNAYQQHAVAPYSLRALPGAPVAAPLEWDEALSSQWDPRRWTIKNLFRR
                     LSQRDDPWS"
     gene            complement(2480..2797)
                     /locus_tag="VFP02_03795"
     CDS             complement(2480..2797)
                     /locus_tag="VFP02_03795"
                     /inference="COORDINATES: protein motif:HMM:NF017880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HET9542178.1"
                     /translation="MRHLHHIMTLEDWDRFEASGLSVQSTRDRTLAEEGFIHCSYTEQ
                     VEGTLNRFYGDLDDVVVLTLDPGRLDADVIDEPAADGTQFPHVYGPLTLGAVTEVVRR
                     KPG"
BASE COUNT          491 a         1049 c          986 g          432 t
ORIGIN      
        1 cgggccgaca tcagggcctt ctacggcgag gtgttcgact tcgaggccct cgacgtcccg
       61 atcctcggtc agctcgggct cctcctgcgc accgacgagg cgacgagcca gttcctcctg
      121 ctcatggagt ccgagacgcc gatcgacgcg cccggctatg accacctcgg gttcctctac
      181 gacacgcgtg agcaggtcga cgaccggctc gagcaggtga aacgctggca ggagaaggac
      241 gaacgggtcc gcatcaagga gtacgaggac ctggtcaccg gcgcgacaac cgtgcacgcg
      301 ttctacgtgc ggtacctgct gcccatctgg ttcgacgtcc agagcatcga acacgccgga
      361 cccatcccgt cccgagagga ttgacatggc catcgacttc acgctgacgc ccgagttgga
      421 agagctgcgg accaaggtcc ggaccttcct cgacgaggtg gtcgtcccca cggagaagac
      481 gctcgacccc gacgaccgga gcgcctacct gaccgcgctg ctgggcatgc gcaaggcggc
      541 gttcgaggcg gggctctggt gcccgcacat gcccgaagag tggggcggca tgggcctcag
      601 ccacgtcgag atggccatgg tccaggccga agcggcgaag acctactacg gcccgtgggt
      661 cctgaactgc caggcccccg acgagggcaa catgcacacc ctcctgcact gggccacccc
      721 tgagcaggcc gagaagtacc tccgccccct gtgcgacggc aaggcctggt cctgcttcgc
      781 catgaccgag cccgaggtcg ccggctcgga cccgaccctg atccagaccc gggcctacca
      841 ggacggtgac gagtgggtca tcaacggcca caagtggttc atctccaacg cccaccgcgc
      901 caacttcgcc atcctcatcg cccgcaccga ggacgacccc gacctgcccc aggcggccaa
      961 ctcggccttc atcgtcgaca tccccagcga cggctggacc gaggtccgtc aggtcgagac
     1021 catgcacggc tcgaccggcc actccgagat cctcatcgag gaccttcggg tccacgacga
     1081 ccagatgctc ggtggccgcg gggagggcca cctgctcggc cagtcccgcc tcggcccggc
     1141 ccggctggcc cactgcatgc gttggatcgc ccaggccgag acggcgctgg acatgatggt
     1201 cgaccggtcc ttgaaccggt tctcccacgg gtcgatcctg gccgagaagc agggcgtcca
     1261 gtggatgatc gccgactcgg ccatggagct gtaccaggcc aagctcatgg tcctccacgc
     1321 cgcctacaag atcgaccgcg gggacgactt caagtccgag gtctcgatgg ccaagcactt
     1381 cgtggccaac gccctgaacc gcatcatcga ccggtcgatc caggtccacg gggccctcgg
     1441 ctactcgacc gacaccccgc tggcgggcat gttccagcac gcccgctggg cccgcttcgc
     1501 cgacggcgcc gacgagatcc accagatgcg catcgcccag cgcacgatcg ccgcctacaa
     1561 ggacttcggc accaccaaag cggccaccgg cgacctgccg atctagccgt cgtcaggacc
     1621 aggggtcgtc gcgctgggac aggcggcgga agaggttctt gatcgtccac cgccgaggat
     1681 cccattggga ggaaagcgcc tcgtcccact ccagcggcgc ggcgaccgga gcaccgggca
     1741 gggcccgcag cgagtacggc gccaccgcgt gctgctggta ggcgttgcgg ttgatgtcga
     1801 gcaggagccg gtcgccccgc ttctccttgt agaactcgag ggtgtactcg tcgggctcct
     1861 cggcgacgag ctgctcgctg acctcgaggg ccaagccgcg ggcggcgggg aagtcgggaa
     1921 ggtcgacatc gaccaccacg tgcagccccc gggagccgct ggtcttcacc cgagcggtgt
     1981 agccccggtc gccgaagagc tcgcgcaagc gccccgcggc gtggcgcacg ggcccccggt
     2041 ccggggtcga ggggtcgagg tcgaccatga cctcgaccgg tcggtacggg tcgtcggccc
     2101 ggctgaacag ggtgtgcagg acgaggacgc cctggccggc caggtagacg acggtggccg
     2161 cgtcctcgat cacgacgaac tcggtgctcc cgtcccgggt ggtcagctcg acccggggga
     2221 tccagtcggg gaagtgcttg cccacctgct tctggaagaa cccgcccttg ccgatgccgt
     2281 cggggaagcg atgaagggcg accgggcgcc cctgggcgtg ccggaccagc agaggcgcga
     2341 tgcgctcgta gtactggacc atctccgcct tggtgatgcc gtcgtcgggg aacagcacct
     2401 tgtccgggtt ggagatcgac agctcacggc cgtcgacctc gagctccaag cgtcttgctt
     2461 tggccgccat ggacgggacc taccccggct tccgccggac gacctcggtc accgcaccca
     2521 gcgtcaaagg gccgtagacg tgcgggaact gcgtcccgtc ggcggcgggc tcgtcgatca
     2581 cgtcggcgtc gaggcgcccg gggtccaggg tgagcacgac cacgtcgtcc aggtcgccgt
     2641 agaagcgatt gagtgtcccc tccacctgct cggtgtagga gcagtggatg aagccctctt
     2701 cggccaacgt gcggtcgcgg gtggactgga cggacaggcc ggaggcctcg aaccggtccc
     2761 agtcctcgag ggtcatgatg tgatggaggt ggcgcaccgc cttcagcctc gcaggtactg
     2821 gtcggccgtg gccccgagcg cgtcggcgaa gccttcgacc aggccggcca ggatctcggc
     2881 caccggcagg acgccgtcga tgcgcccggc cccctgaccc gccatcggga tcgccgcctc
     2941 catgtccccg ccgaagta
//
LOCUS       DASTFV010000200         2422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_761648, whole genome shotgun sequence.
ACCESSION   DASTFV010000200 DASTFV010000000
VERSION     DASTFV010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761648"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..893
                     /locus_tag="VFP02_03800"
     CDS             135..893
                     /locus_tag="VFP02_03800"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET9542179.1"
                     /translation="MTEHQEIEELLGAYALDAVDPDEARLVEEHLVECPRCRAEVAAH
                     REVAALLTSGTSDPVPDGVWDRIAADLGDTPPPVPIEVAFGERQARKTTARTRPARVL
                     AGLAAAAAVVVVALMGAVLVNQRTEIDDLRDEAAAPEADPVLDLLRDPTSQIVSLDHA
                     DGTTGARALVGGDGDSVLLASGLPDLGEGETYQLWGLPQGRESMVSLGVLGADPGQSE
                     FHVEGGITTLAITREPGGGSTQPSSEPLVTGTLV"
     gene            986..2206
                     /locus_tag="VFP02_03805"
     CDS             986..2206
                     /locus_tag="VFP02_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542180.1"
                     /translation="MALVAVVLLLATACSDDDEDISELPPNATATPAAGETSTTVAAD
                     EPPGESAAASLLSDLTGLFEEQVYLTGFTVQAAAAADGDLDDPEAAAMADATAASSVE
                     LSDVVGAAYGVAAGVDFNEAWDAHQATLVDFGLRGGTAEAVTTARDAVVSTLEGFDPE
                     GDFSTVAAGLEASDGEMLTTVEELASQAPAAAVDLRAAADEMPEVALGLADVIASHSV
                     LEGEVDSPESALRADLTGLMQESALLTGLALSEIVQADGDSSAPGPRGVLAAIDENTV
                     ALAEAMVPEDEAERDAFAETWGGHIGDFEDYITAVMADEAAGIQEAEDAIVTFRDDLG
                     VVLSDTYPAFTREAVAEELVPHTDSIIAVADALVAEADGATPIESPDLLRAAALAMRD
                     AARYFAGGLSAPTE"
BASE COUNT          347 a          885 c          873 g          317 t
ORIGIN      
        1 tacttcggcg gctacaccta tcgggaggtg gccgagatgc tcgacgagcc cgaagggacc
       61 atcaagggcc gcatccgtac cggcctccac cgcctgcgtg ggggcctgca cgccgccggg
      121 atcggagtcg aagggtgacc gagcatcagg agatcgagga gctgctcggg gcctacgccc
      181 tcgacgcggt cgatcccgac gaggcccgcc tggtcgaaga gcacctggtc gagtgccccc
      241 gctgtcgggc cgaggtggcc gcgcaccgcg aggtggcggc cctgctgacc agcggcacga
      301 gcgatccggt gcccgacggg gtctgggacc gcatcgccgc ggacctgggc gacaccccgc
      361 cccccgtgcc gatcgaggtc gccttcggcg agcgccaggc ccggaagacg accgcccgga
      421 cgcgcccggc ccgcgtcctg gccggcctgg ccgcggctgc ggccgtggtc gtggtggccc
      481 tgatgggcgc cgtgctggtc aaccagcgca ccgagatcga cgacctccgg gacgaggccg
      541 cggcaccgga agccgatccc gtcctggacc tgctccgcga ccccacgtca cagatcgtga
      601 gcctggatca cgccgacggc accaccggag cccgcgccct cgtgggcggc gacggcgaca
      661 gcgtgctgct cgcctcgggc ctgcccgacc tgggcgaggg tgagacctac cagctctggg
      721 gcctgcccca ggggcgggag tcgatggtct cgctgggcgt gctgggggcc gaccccgggc
      781 agtccgagtt ccacgtcgaa ggcgggatca cgaccttggc catcacccgg gagcccggcg
      841 gcggatccac ccaacccagt tcagagcccc tggtcacggg cacgttggtc tgaatcgcgg
      901 cccgtcccga ctggcgcgat gccctgacct gggtgcgagg atggggccaa tggtcacccg
      961 aactccctca cgcgccacgt ccctgatggc tctcgtcgcc gtcgtcctcc tgctggcgac
     1021 cgcctgcagc gacgacgacg aggacatcag cgaactaccg cccaacgcga ccgccactcc
     1081 cgccgccggt gagacgtcca ccacggtcgc cgccgacgag cctccgggcg agagcgcggc
     1141 ggccagcctc ctctcggacc tgaccggact gttcgaggag caggtgtacc tgaccggctt
     1201 caccgtccag gcggccgcgg ccgccgacgg cgacctggac gacccggaag cggcggccat
     1261 ggccgacgcc acggcggcca gctcggtcga gctgtccgac gtggtcggcg ccgcgtacgg
     1321 cgtggctgcg ggtgtcgact tcaacgaggc ctgggacgcg caccaggcca ccctcgtcga
     1381 cttcggcctg cgtgggggga cggccgaagc ggtgacgacg gcccgggacg cggtcgtgtc
     1441 gacgctggag ggcttcgatc ccgaggggga cttctccacc gtggccgccg gcttggaagc
     1501 gagtgacggc gagatgctga ccaccgtcga ggagctggcc tcgcaagctc ctgcggccgc
     1561 cgtcgacctg cgcgcggcgg ccgacgagat gcccgaggtg gccctcggcc tggccgacgt
     1621 catcgccagc cacagcgtgc tggagggcga ggtcgactcg cccgagtccg ccctgcgggc
     1681 cgacctcacc ggcctcatgc aggagagtgc gctgctgacc ggcctggccc tgtccgagat
     1741 cgtgcaggcc gacggtgaca gctctgcccc cggcccccgc ggggtcctgg ccgccatcga
     1801 cgagaacacc gtggcgctgg ccgaagcgat ggtccccgag gacgaggccg agcgcgacgc
     1861 cttcgctgag acctggggcg gccacatcgg cgacttcgag gactacatca ccgccgtgat
     1921 ggccgacgag gccgccggca tccaggaggc ggaggacgcc atcgtcacgt tccgggacga
     1981 cctgggcgtc gtcctgtccg acacctaccc ggccttcacc cgcgaggcgg tggccgagga
     2041 gctggtgccg cacaccgact cgatcatcgc cgtcgccgac gcgctggtgg cagaggccga
     2101 cggggccacc cccatcgagt ccccggacct cctccgggct gcggccctgg ccatgcgcga
     2161 cgcggcccgc tacttcgccg gagggctcag cgcgccgacc gagtgatcgg cttccgcgcc
     2221 gaggtgtgac aggtgtcacc tgcgggtatg cgcaactccg tcatgggtag tgtcgaagaa
     2281 ggggggaacg ccatgttgcc gggggacgaa cattgaacgt caacttcttc ggggttcggg
     2341 gctccacccc gtgtgcctgc agggatctgg cccgctacgg gggcaacacc gcctgcgtgg
     2401 ccctggaagc cccggggcac ga
//
LOCUS       DASTFV010000201         3397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_764681, whole genome shotgun sequence.
ACCESSION   DASTFV010000201 DASTFV010000000
VERSION     DASTFV010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764681"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VFP02_03810"
     CDS             <1..491
                     /locus_tag="VFP02_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542181.1"
                     /translation="PAPPPGAPGAVPPPPGAPVPGVVPPPAAAAPLAGGPDPHGLGAA
                     ASRLSGGARKNGRAALAVLSVVLEDADVVEQVLVGRYHGVDGAAALASGNLVLVNVRE
                     WQPEVVRVSLVGMGVQGTQDGRKATLAFTSGATQDRFDEVGDTALAVEFANRIRQRAT
                     GG"
     gene            523..598
                     /locus_tag="VFP02_03815"
     tRNA            523..598
                     /locus_tag="VFP02_03815"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:556..558,aa:Gly,seq:gcc)
     gene            660..2093
                     /locus_tag="VFP02_03820"
     CDS             660..2093
                     /locus_tag="VFP02_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET9542182.1"
                     /translation="MTDDLARLDAVAQAALVRSGEVSPLELVDAAIGRIEALNPQLNA
                     VIHERFERARDEAASPDLPDGPFRGVPFLLKDLWPTSAGDPFHMGIKAFKEAGYIHPE
                     DANLTKRYREAGFVIVGRTNTPELGLVATTEPLAYGPTHNPWNPDHGPGGSSGGSAAA
                     VASGMVPCANASDGGGSIRIPAAMCGLVGLKPSRGRGSMGPLRDEWGVSVQHVVSHSV
                     RDTAAVLDATWAPFPGDGVIAPAPARPWLDEIGMTEPLRIGILDHVLGGVPTHAACTA
                     SARRGAELAAALGHHVEEAYPAEMDRMAEHGPLVTTMWAVGATASLLSLAPLLGRELT
                     EDDVEPGTWALAEWGNSASAVELASAQGKMAVVRRALAAWWEDYDLLITPTTAAPPPL
                     LGQLVATDDEPLRGLIGSTPYATFTAVFNQSGQPAISLPLHRTADGLPVGTQIVAAYG
                     REDLLIRIAAQLEAEVGWADERAPIHA"
     gene            2139..2213
                     /locus_tag="VFP02_03825"
     tRNA            2139..2213
                     /locus_tag="VFP02_03825"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2172..2174,aa:Cys,seq:gca)
     gene            complement(2228..3118)
                     /locus_tag="VFP02_03830"
     CDS             complement(2228..3118)
                     /locus_tag="VFP02_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542183.1"
                     /translation="MTRTTNTGRRFVFRPVGVFSLAAAKAFVGRFAPFMRPEAAAEVG
                     DDGLILAFLSDDGRPVAVRLHQTAERVVADVLEGDGGDGLRAQVARILSLDTDAGPYE
                     AIEDEVITRVRDQHPGLRPVHYPTPYEAATWAVLIQRTQRVQAANIRRKLAERHGAVV
                     TIDGVDHLTAIPPDEMAAIREVWGVPAVKVPWLRAVGRAELEGDLDAESLRAMDPAEA
                     LARLREIDGIGPFGAELILVRGAAAPDVFARNEPLLHRVMTHHYGLADPSRADLVAIA
                     ERWRPRRSWATFVLRQATPR"
BASE COUNT          457 a         1215 c         1244 g          481 t
ORIGIN      
        1 cccctgcgcc tccccccggt gcaccgggtg cggtgccgcc tccgcctggc gccccggtcc
       61 cgggggtcgt gccgccgccc gccgcggcag cgccgctcgc cggcgggccc gaccctcacg
      121 gcctgggcgc ggctgcctcg cgcctgtcgg gcggcgcccg caagaacggt cgtgcggcgc
      181 tggccgtcct gagcgtggtc ctcgaggacg ctgacgtggt cgagcaggtg ctggtcggca
      241 ggtaccacgg cgtcgacggt gccgccgcgc tcgcgtccgg gaacctcgtg ctcgtcaacg
      301 tccgcgagtg gcagccggag gttgtccgcg tgtcgctggt cgggatgggc gtccagggca
      361 cccaggacgg gcgcaaggcc acgctggcgt tcaccagtgg ggccacgcag gaccgcttcg
      421 acgaggtcgg cgacacggcg ttggcggtcg agttcgcgaa ccggatccgt cagcgggcca
      481 ccggggggtg agcgaggggg ctcgctacac tctttggtcc ttgcggtagt agctcagttg
      541 gtagagcgca accttgccaa ggttgaggtc gccggttcga gcccggtcta ccgctccacg
      601 aacgtccccc accgtggtgg gggacgtcgt cgttttcggg tgcggtctac ggtccagcca
      661 tgaccgatga tctcgcccgc ctcgacgccg tggcccaggc cgccctggtc cgttcgggcg
      721 aggtctcacc cctcgagctg gtggacgccg cgatcgggcg catcgaagcc ctgaaccccc
      781 aactgaacgc cgtcatccac gagcgtttcg agcgggcccg ggacgaagcg gcgtcgcccg
      841 acctgcccga cgggccgttc cggggcgtgc cgttcctgct caaggacctg tggccgacct
      901 cggccggcga cccgttccac atggggatca aggccttcaa ggaggcgggc tacatccacc
      961 cggaggacgc caacctgacc aagcgctacc gggaggccgg cttcgtcatc gtgggtcgga
     1021 ccaacacgcc ggagctgggg ttggtggcca ccaccgagcc attggcctac gggccgacgc
     1081 acaacccgtg gaaccccgat cacggccccg gcgggtccag cgggggatcg gcggccgccg
     1141 tggcctcggg catggtgccc tgcgccaacg cgtccgacgg cggggggtcc atccgcatcc
     1201 cggccgccat gtgtgggctc gtcggtctca agccctcccg ggggcggggc tcgatgggcc
     1261 ccttgcggga cgagtggggc gtctcggtcc agcacgtggt ctcgcactcg gtgcgggaca
     1321 ccgccgcggt gctggatgcc acgtgggcgc cgttccccgg cgacggcgtg atcgcccccg
     1381 ccccggcccg gccgtggctg gacgagatcg ggatgaccga gccgttgcgc atcggcatcc
     1441 tcgaccatgt cctcggtggg gtccccaccc acgccgcgtg cacggcgtcg gcccggcggg
     1501 gggcagagct ggccgccgcc ctcggtcacc acgtcgagga ggcctacccg gcggagatgg
     1561 accgcatggc cgagcacggc cccctggtga cgacgatgtg ggcggtcggc gccacggcga
     1621 gcctgctctc cctggcgccg ttgctgggcc gggagttgac cgaggacgac gtcgagccgg
     1681 gcacctgggc cctggccgag tggggcaaca gtgcttcggc ggtcgagttg gcgtcagccc
     1741 aggggaagat ggccgtcgtg cgccgggcct tggccgcgtg gtgggaggac tacgacctgc
     1801 tgatcacccc gaccaccgcc gcgcccccac cgctcttggg ccagctggtg gccaccgacg
     1861 acgagccgct gcggggcctg atcggctcga cgccctacgc caccttcacc gcggtgttca
     1921 accagagcgg tcagcccgcc atctcgctgc ccctgcaccg gacagcagat ggcctaccgg
     1981 tcggcaccca gatcgtggcc gcctacggcc gggaggacct gctcatccgc atcgcggccc
     2041 agctcgaggc cgaggtcggt tgggccgatg agcgggcccc gatccacgcc tgaaagatcg
     2101 ggccgggcgg cgggccgccg gtagggtgac cgcttcctgg cggcgtggcc gagtggttta
     2161 ggcaggggcc tgcaaagccc cgtacccggg ttcgattccc ggcgccgcct ccagagcgtg
     2221 caggccctca gcggggtgtg gcctggcgga gcacgaaggt ggcccaggac cggcgggggc
     2281 gccagcgttc ggcgatggcg acgagatcgg cccggctggg atcggcgagg ccgtagtggt
     2341 gggtcatcac ccggtgcagc agcggctcgt tgcgggcgaa cacgtccgga gccgcagcgc
     2401 cccgaacgag gatcagctcg gctccgaagg gaccgatccc gtcgatctcc cggaggcggg
     2461 ccagcgcctc cgcaggatcc atggcccgca gcgactcggc gtcgaggtcc ccttcgagct
     2521 cggcccggcc gacggcccgc agccacggca ccttcaccgc gggcacgccc cacacctctc
     2581 ggatcgccgc catctcgtcc ggcgggatcg cggtgaggtg gtcgacaccg tcgatggtca
     2641 ccactgcgcc gtgacgttcg gccaacttgc gccggatgtt ggcggcctga accctctgcg
     2701 tgcgctggat cagcacggcc cacgtcgccg cctcgtaggg cgtcgggtag tgcacaggcc
     2761 gcagcccggg gtgttgatcg cggacccgag tgatcacctc gtcctcgatg gcttcgtagg
     2821 gaccggcgtc ggtgtccagg gacaggatcc gggccacctg ggcccgcaga ccgtcgccgc
     2881 cgtcgccctc gaggacgtcc gcgacaaccc gttcggcggt ctggtgcagc cggacggcga
     2941 ccggccttcc gtcgtcgctc aggaaggcga ggatcaagcc gtcgtcccca acttcagcgg
     3001 ccgcctccgg ccgcatgaac ggggcgaagc ggccgacgaa cgccttcgcc gccgccagcg
     3061 agaagacacc gaccgggcgg aacacgaacc ggcggccggt gttcgtcgtt cgggtcacgg
     3121 aggtggtcta cccacggatc cgcccctctc ggcggccaga gccgagggtc gacgggctcg
     3181 ctctcggacg gcgaggacgc cgaacacgtt ccccggtggg gcaaggcctg aggtcgggcc
     3241 gaactccgag aagtcgcttc ggagccgtcc ttgagtgtca cacccccgga gtagagtggg
     3301 gtacaccagt tcgagaggac cgtggaggag ggtgaccggc cgtgatccgt gagctcgctg
     3361 acctcgagca ctacctggac ctggccaaca aggccac
//
LOCUS       DASTFV010000202         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_767196, whole genome shotgun sequence.
ACCESSION   DASTFV010000202 DASTFV010000000
VERSION     DASTFV010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767196"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..809
                     /locus_tag="VFP02_03835"
     CDS             45..809
                     /locus_tag="VFP02_03835"
                     /EC_number="1.1.1.333"
                     /inference="COORDINATES: protein motif:HMM:NF005912.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenylphospho-beta-D-erythro-pentofuranosid-
                     2-ulose 2-reductase"
                     /protein_id="HET9542184.1"
                     /translation="MKDALGAVQTALVLGGTSEIGLATVRRLAQDRCRRVVLAVRDPA
                     AAEPVADALRADGVGEVHVIAFDAREPANHTAVIDEAVALLGDLDLVLLAFGVLGDQE
                     AFDADPASAADAVAVNLSGAVSSGLAAANRLRAQGHGTLVVLSSVAGERVRAENLVYG
                     ATKAGLDGFAQGLGDALQGSGARVMVVRPGFVHTRMTEGRDPAPFATTPEAVADEIAK
                     GLAKGSDTVWAPPILRFVFSTFRHLPRPVWRKVSAR"
     gene            964..1863
                     /locus_tag="VFP02_03840"
     CDS             964..1863
                     /locus_tag="VFP02_03840"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HET9542185.1"
                     /translation="MSPRREGVVGGLIRAARPKQWAKNLLVFAAPAAAGVLDQAEPLA
                     DATIAFGAFCLAASGTYMLNDVADREADARHPTKHTRPIAAGIVPPGLATVVGVLAIA
                     SGLALSFAADWHLAVTVAIYVAITTAYSYWLKHVVILDVVAVASGFVLRAIGGATATG
                     VPVSDWFFIVTSFGSLFVVTGKRQGEVGEMAEEEGAAADVRKTLDEYSDSFLAYLRTI
                     SSAVMFMAYCVWAFETAAASSTSVPWFELSILPFVIAVLRYALLLDRGHGAAPEELFF
                     ADRPLQLAGLAWALVYGTAVYLT"
     gene            1916..>2367
                     /locus_tag="VFP02_03845"
     CDS             1916..>2367
                     /locus_tag="VFP02_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9542186.1"
                     /translation="MSQELTGWSRTAPTRATVVSPTTPETLAATLADVGDRGVIARGL
                     GRSYGDAAQNAGGAVADATGVRRLLAFDGVTGLVTAESGLSFDDLLRVVVPQGWFVPV
                     SPGTRHVTVGGAIAADVHGKNHHRDGSFVQHVRSLRLRTPAGVVEVGP"
BASE COUNT          276 a          921 c          809 g          361 t
ORIGIN      
        1 caggtcctcc tggcctcctg atccaggccg taccctcctg cgccatgaag gacgcgctcg
       61 gagccgtgca gaccgcgttg gtgctcggag gcacgtcgga gatcggcctg gccaccgtcc
      121 gccgcctggc ccaggaccgt tgtcgccggg tcgttttggc cgtgcgagac ccggctgccg
      181 ccgagcccgt ggccgacgcc ctgcgggccg atggcgtcgg cgaggtccac gtcatcgcct
      241 tcgacgcccg cgaaccggcc aaccacacgg cggtgatcga cgaggcagtc gccctgctcg
      301 gtgacctcga cctcgtcctc ctcgcgttcg gtgtgctcgg cgaccaggag gcattcgacg
      361 ccgacccggc gtcggcggcc gacgccgtcg ccgtcaacct ttccggggcg gtgtccagcg
      421 gcctcgccgc cgccaaccgc ttgcgcgccc agggccacgg gacgctggtc gtcctgtcct
      481 cggtcgccgg cgagcgggtc cgggccgaga acctcgtcta cggcgccacc aaggccggcc
      541 tggacgggtt cgcccagggc ctgggcgacg ccctgcaggg ctcgggcgcc cgcgtgatgg
      601 tggtccgccc cgggttcgtc cacacccgca tgaccgaggg ccgcgacccg gccccgttcg
      661 ccaccacccc cgaggcggtg gccgacgaga tcgccaaggg cctggcgaag gggtccgaca
      721 cggtctgggc cccgcccatc ctccgcttcg tgttcagcac ctttcgccac ctgccccgcc
      781 cggtctggcg caaggtctcc gcccgctgat cctggtgccc accagcccag cggtcggctc
      841 ctggatcgaa ggcgtgaccc gacggaggct cggtgtgagg cggcccgcct acgctctggc
      901 catgagcccg gcgcgtgacg agccgacgcg ccggcaccgc gtcgaccgcc gggcgccttg
      961 agcgtgtccc cgcgccggga aggcgtcgtc ggcggcctga tccgggccgc ccggccgaag
     1021 cagtgggcca agaacctctt ggtgttcgcc gcccccgccg cggccggcgt cctcgaccag
     1081 gccgagccgc tggccgacgc cacgatcgcc ttcggcgcct tctgcctggc cgcctcgggc
     1141 acctacatgc tcaacgacgt cgccgaccgg gaggccgacg cccgccaccc gaccaagcac
     1201 acccggccca tcgccgccgg gatcgtcccc ccggggctgg ccacggtcgt cggggtcctc
     1261 gccatcgcca gcggcttggc cctcagcttc gccgccgact ggcacctggc cgtcaccgtc
     1321 gccatctacg tcgcgatcac tacggcctac agctactggc tgaagcacgt ggtcatcctc
     1381 gacgtcgtcg ccgtcgccag cgggttcgtg ctgcgggcca tcggcggggc caccgcaacg
     1441 ggtgtcccgg tgtcggattg gttcttcatc gtgacctcgt tcggctccct gttcgtggtg
     1501 accggcaagc gccagggcga ggtcggcgag atggccgagg aggaaggcgc agccgcagac
     1561 gtgcgcaaga ccctcgacga gtacagcgac tcgtttctcg cctacctgcg cacgatctcg
     1621 tccgcggtga tgttcatggc ctactgcgtg tgggccttcg agacggctgc ggccagttcg
     1681 acctccgtgc cgtggttcga gctctcgatc ctgcccttcg tgatcgccgt ccttcgctac
     1741 gccctgctcc tcgatcgcgg ccacggcgcc gccccggagg agctgttctt cgccgatcgg
     1801 ccgctccagc tggccggcct ggcctgggcc ctggtctacg gcacggccgt ctacctgacg
     1861 tgatgccggg gcggaaccct gtcgtggtca cccgtcgccg ccgacaggac cagcgatgag
     1921 ccaggagctg accggctggt cgcgcacggc cccgacccgg gccacggtgg tctcccccac
     1981 cacccccgag acgctcgccg ccaccttggc cgacgtcggt gaccggggcg tgatcgcccg
     2041 cgggctaggt cggtcctacg gcgacgccgc ccagaacgcg ggtggggcgg tggccgacgc
     2101 caccggggtg cgccggctgc tggccttcga cggcgtcacc ggcttggtca cggccgagtc
     2161 gggcctgagc ttcgacgatc tcctgcgggt ggtcgtgcct cagggctggt tcgtgcccgt
     2221 ctcccccggc acccgtcacg tcacggtcgg gggcgccatt gccgccgacg tccacggcaa
     2281 gaaccaccac cgggacggct ccttcgtcca gcacgtccgc tcgctgcgcc tgcgcacgcc
     2341 ggccggggtc gtcgaggtcg gcccgga
//
LOCUS       DASTFV010000203         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_768464, whole genome shotgun sequence.
ACCESSION   DASTFV010000203 DASTFV010000000
VERSION     DASTFV010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768464"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFP02_03850"
     CDS             complement(<1..435)
                     /locus_tag="VFP02_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542187.1"
                     /translation="MKRLLVSLALLFGLIAGPAAVLPTGAGATAPAEGSCGVTWGSVA
                     KSDGPGTTPSSRVHNVRTGRHECYDRFVVDLTGSARGYNVRYVRRFRQDPSGDVIPLP
                     GGARIEIVIRAPAVDLNGHATYPGVVGEPLPGVNLAGYETFRS"
     gene            565..1125
                     /locus_tag="VFP02_03855"
     CDS             565..1125
                     /locus_tag="VFP02_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET9542188.1"
                     /translation="MTNQLQDRRIAFLVSNEGAEQVELTEPWSAVEDAGGTPVLVAPE
                     AGEVQAFNHLDKGDTFPVDETLTDADPASFDGLVLPGGVANPDQLRTDDDAVAFVRAF
                     FDAGRPVAAICHAPWTLVEADVVDGRAVTSWPSLQTDLRNAGAQWTDEEVVIDDTGPN
                     TLVTSRKPDDLKAFSEAAIEAFAGSE"
     gene            1153..1671
                     /locus_tag="VFP02_03860"
     CDS             1153..1671
                     /locus_tag="VFP02_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9542189.1"
                     /translation="MPVLRLNHAVLYVRDAQRAAAFYEVVLGFRRIRMVDGFDGAVFL
                     QAPGSTNDHDLGLFTIGEGAGPSGAGRQTVGLYHLAWEVDTLAELERIAGLLSEHGAL
                     VGASDHGTTKALYAHDPDGIEFEVSWLVPADLLDEATLEARARIGPLDIETEKRRYGA
                     ETRGGVGVSAPA"
     gene            1721..1814
                     /locus_tag="VFP02_03865"
     tRNA            1721..1814
                     /locus_tag="VFP02_03865"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1757..1759,aa:Ser,seq:gct)
BASE COUNT          322 a          712 c          727 g          299 t
ORIGIN      
        1 ggagcggaac gtttcgtacc cggcaaggtt cacgccgggc agcggctcac cgacgacgcc
       61 cggataggtc gcgtggccgt tgaggtcgac cgcgggggcg cggatgacga tctcgatgcg
      121 ggcaccgccc ggcagcggga tgacgtcgcc ggacgggtcc tggcggaagc gccggacgta
      181 gcggacgttg taaccccggg ctgagccggt caggtcgacc acgaagcggt cgtagcactc
      241 gtgccgcccg gtgcggacgt tgtgcacccg cgacgaggga gtggtccccg gcccgtcgct
      301 cttggccacg ctgccccagg tcaccccgca cgagccctcc gccggggccg tggcaccggc
      361 ccccgtgggc aggaccgccg ccggaccggc aatcaggccg aagaggaggg caagggacac
      421 caggagtcgc ttcatgcgtc tgtcctacac cctggcgacc cttgcgtcac gagatgcgtc
      481 cccgcacccg ggcggtcggg tgatcgcact caccctggtg ggcggctcgg agttgaccga
      541 cctctcgacg ggtagggggg agccatgacc aaccagctgc aggaccgacg tatcgccttc
      601 ctcgtcagca acgagggggc cgagcaggtg gagctgaccg agccttggtc agccgtggag
      661 gacgctggcg gcacgcccgt cctcgtggcg ccggaggccg gcgaggtgca ggcgttcaac
      721 cacttggaca agggcgacac gttcccggtc gacgagacgc tgaccgacgc cgacccggcg
      781 agcttcgacg gtctggtcct ccccggcggg gtggccaacc cggaccagct ccgcaccgac
      841 gacgatgcgg tcgccttcgt gcgcgccttc ttcgacgcgg gccggcccgt ggccgccatc
      901 tgccacgccc cgtggaccct ggtcgaggcc gacgtcgtcg acgggcgggc ggtcacgagc
      961 tggcccagcc ttcagaccga ccttcgcaac gccggcgccc agtggaccga cgaggaggtc
     1021 gtgatcgacg acacggggcc gaacacgctg gtgacgagcc gcaagcccga cgacctgaaa
     1081 gccttctccg aggccgcgat cgaggccttc gccggctcgg aataggccac accgggagac
     1141 gttcaccagg acatgcctgt cctccgtctg aaccacgccg tcctctatgt gcgggacgcg
     1201 cagcgcgccg ccgccttcta cgaagtggta ctcggcttcc gtaggatccg catggtcgac
     1261 ggtttcgacg gggccgtgtt cctgcaggcc cccggctcga ccaacgacca cgacctcgga
     1321 ctgttcacca tcggcgaagg cgccgggccg tctggcgccg gccgccagac cgtgggtctc
     1381 taccacctgg cctgggaggt cgacaccctg gccgagttgg agcgcatcgc cggcctcctg
     1441 tccgagcacg gcgctctggt cggcgccagt gaccacggca cgaccaaagc cctgtacgcc
     1501 cacgatccgg acggcatcga gttcgaggtg tcctggctgg tccccgccga cctcctcgac
     1561 gaggcgacgt tggaggcgag ggcccgcatc gggcccctcg acatcgagac ggagaagcgc
     1621 cgctacggag ccgagacccg cggcggtgtg ggggtgtcgg cccccgcctg accgtgggcg
     1681 gggatgcacc ggtagtggct cgctaccatc cccaccttca ggagaggtgc cggagtctgg
     1741 ctgatcgggg cgccctgcta aggcgttagt gaccgcaagg ttgctcgtgg gttcaaatcc
     1801 caccctctcc gccagatgga tcaggtttga accgcagtcg gctcgggacc ccaccacgac
     1861 gggcccagcc atgccgccga tggcgctgtc cagagccgat ttgttgtcac tagcgtttgg
     1921 gtatggcgtt ccaggggtgg ccggtggagg cgctggagtt ctacgagggc ctcgaagtcg
     1981 acaacaccaa ggcgtactgg accgaccaca aggcggtgta cgaggacaag gtccgggccc
     2041 cgatggaggc gttgctggcc
//
LOCUS       DASTFV010000204         4299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_769099, whole genome shotgun sequence.
ACCESSION   DASTFV010000204 DASTFV010000000
VERSION     DASTFV010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769099"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VFP02_03870"
     CDS             complement(<1..635)
                     /locus_tag="VFP02_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET9542190.1"
                     /translation="MRCRTERGQALPLMLVIMAAAVGAMLLLGGLGRRAVEGAQAQTA
                     ADAAALAAAAEGPPAAEELARLNGSTPADVEGTVGDATVTVRVGGADAVARAQSPPPP
                     AGGGTVGLAPDMVAAVTRAGALLGEPVPISSGWRSRAQQAALWARRSTNPYPVARPGT
                     SMHERGLAIDVHRAFVARLRLVATAAGLCQPLPSADPVHFELCRRTLVTSLA"
     gene            complement(687..3206)
                     /gene="gyrA"
                     /locus_tag="VFP02_03875"
     CDS             complement(687..3206)
                     /gene="gyrA"
                     /locus_tag="VFP02_03875"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET9542191.1"
                     /translation="MTPSPPPDGRDETLFEEEFEPIEIQEEMERSFLDYSMSVIVSRA
                     LPDARDGLKPVHRRILWGMHDLGARPDRPTIKCARVTGDVMGKYHPHGDSAIYDALVR
                     MGQSFSLRHPLIHPKGNFGTPDDPPAAARYTECRLAEIALAMLDGIDEDTVDFVDNYS
                     GEFQEPSLLPARFPNLLVNGSQGIAVGMATNIPPHNLGEVIDATVHLIDHPDATPDDL
                     MAFIKGPDFPTGALMMGRQGILDAYRTGRGSIRLRAVAEIEEGKRNDMIVVSKLPYQV
                     SPSTVLMKIKELVDSGELTGIADINNASAGEEVRLEIKLKKDAPALVILNNLYKRTPL
                     QTNFAVNTVALIDGVPRTLNLVQALQAYIDHQVNVITRRSEYRLAEAKAREHIVEGLL
                     KAIDMIDAIIALIRASADRAEARQGLMGEGFEFTEIQANHILDMQLGRLTRLGRTDLE
                     KELAELRATIADLEAILNDEGKLRSVIKDELGEVREKHATDRQTELTHDPGEMNLEDL
                     IDDEELVVTYSAAGYIKTVQADAFRTQGRGGRGVAGAKLRDDDYVTEVISTTAHAYLL
                     FFSNRGKVYRLKAHEIPMKDRTARGTAMVNLLALQPDEKIQAIIDTRDYETSRFLFFA
                     TRNGQVKKTKFTEYDSSRRDGLIAITLKDGDELVRVIQTSGDADIFLVARSGQTVRFS
                     EDLVRPMGRSAAGVRGLKLREGDAVVSCAVSEPDADLLCITEGGYGKRTKIDKYPRKG
                     RGTMGVKGFKMTPQKGPVLTGRMVKIDDEVILVSSKGTLIRTAVKQISSQGRDASGVR
                     VMSLEPDETVVAVAPVFSETEDEAKAVPAPTLPVDEAPEPD"
     gene            complement(3203..>4299)
                     /locus_tag="VFP02_03880"
     CDS             complement(3203..>4299)
                     /locus_tag="VFP02_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HET9542192.1"
                     /translation="ANGINTIEGGMHEEGFKKGLTNVVNKYARGKGLLKEKDANLLGE
                     DIREGLTAIISVRLRDPQFEGQTKGKLGNVPMRSLVERATNEKLGDFLEEHPTEANKI
                     VKKSIAAATARTAARDARNATRRKSALEGAGMPDKLRDCQSRNMHETELFIVEGDSAG
                     GPATQARDPRIQAVLPIRGKILNVERARIDKMLKNNEIQALISAIGAGLGEEFDAEKI
                     RYGKVIILCDADVDGSHIRTLLLTFFFRQMRDLVEHGHVYIAQPPLFSTIVGKDKIYL
                     KDDAAKAAFLAENPTHKKEFQRLKGLGEMDWEELQETTIDPTKRTLLKVSVEQAAIAD
                     EVLSVLMGDDVESRKHFIQTNAKDVRFLDI"
BASE COUNT          565 a         1392 c         1563 g          779 t
ORIGIN      
        1 gccaggctcg tgaccagtgt ccgccggcac aattcgaagt gtacggggtc ggccgaaggc
       61 aagggttggc acagccccgc ggcggtggcc acgaggcgca ggcgagccac gaatgcacga
      121 tggacgtcga tggccaggcc ccgttcgtgc atcgaggtcc cggggcgggc caccgggtag
      181 gggttcgtgc tccggcgggc ccacagcgcg gcctgttggg cgcggctgcg ccagcccgag
      241 ctgatcggga caggctcgcc caggagggcg ccggcgcggg tgacggcggc caccatgtcg
      301 ggggccagcc ccacggtccc gccgcccgcg ggcgggggcg gggactgggc gcgagccacc
      361 gcatcggccc ctccgacccg caccgtcacc gtggcgtccc ccacagtgcc ttcgacgtcg
      421 gccggggtgg atccgttcag tcgggccagc tcctccgcgg ccggcggtcc ctcggccgcg
      481 gcggccagcg cggcggcatc cgccgcggtc tgggcctgag cgccctcgac ggcccgtcga
      541 cccagcccac cgagcagcag catggccccg acggccgccg ccatgatcac caacatcagg
      601 ggcagcgcct gcccccgttc ggtcctgcac cgcacccgcg cacctccggc tcaaccgttg
      661 gcggggaaag cggtgcttgg ttgcgatcag tcgggctcgg gggcctcgtc gacggggagg
      721 gtgggggccg gaacggcctt ggcctcgtcc tcggtctcgc tgaagacggg ggctaccgcg
      781 accacggtct cgtccggctc gagggacatg acccggacgc cggacgcatc ccggccctgg
      841 gacgagatct gcttgaccgc ggtgcggatc agcgtgccct tggacgacac caggatgacc
      901 tcgtcgtcga tcttgaccat gcggccggtg aggaccgggc ccttctgggg ggtcatcttg
      961 aagcccttga cccccatggt cccccgcccc ttgcgcgggt acttgtcgat cttggtccgc
     1021 ttgccgtagc cgccctcggt gatgcagagc aggtcggcgt cgggctcgct gaccgcgcag
     1081 gacacgacgg cgtcaccctc gcggagcttc agtccccgca cgcctgcagc tgagcggccc
     1141 atggggcgga cgaggtcctc gctgaagcgc acggtctggc cgctgcgggc caccaggaag
     1201 atgtcggcgt cgccactggt ctggatgacc cggaccagct cgtcgccgtc cttgagagtg
     1261 atggcgatga gaccgtcgcg ccgggacgag tcgtactcgg tgaacttggt cttcttgacc
     1321 tggccgttcc tcgtggcgaa gaacaggaac cggctggtct cgtagtcccg ggtgtcgatg
     1381 atggcctgga tcttctcgtc cggctgcagg gccagcaggt tgaccatcgc cgtcccccgg
     1441 gccgtgcggt ccttcatggg gatctcgtgg gccttgagcc ggtacacctt cccccggttc
     1501 gagaagaaca gcaggtaggc gtgcgccgtg gtcgagatga cctcggtgac gtagtcgtcg
     1561 tcgcgcagct tggccccggc aacaccccgg ccaccacggc cctgggtgcg gaaggcgtcg
     1621 gcttggacgg tcttgatgta gcccgccgcg ctgtaggtga cgaccagctc ctcgtcgtcg
     1681 atgaggtcct cgaggttcat ctcacccggg tcgtgggtga gctcggtctg gcggtccgtg
     1741 gcgtgcttct cgcggacctc gcccagctcg tccttgatga ccgagcgcag cttgccctcg
     1801 tcgttgagga tggcctcgag gtcggcgatg gtcgctcgca gctcggccag ttccttctcc
     1861 aggtcggtac gaccgagccg ggtcaggcgc cccagctgca tgtccaggat gtggttggcc
     1921 tggatctcgg tgaactcgaa gccctccccc atgaggccct gacgggcttc ggcccggtcc
     1981 gccgaagccc ggatgagggc gatgatggcg tcgatcatgt cgatggcctt gagcaggccc
     2041 tcgacgatgt gctcgcgggc cttggcctcg gccaggcggt actcggaccg ccgggtgatc
     2101 acgttgacct ggtggtcgat gtaggcctgc agggcctgga ccaggttcag cgtgcggggc
     2161 acgccgtcga tgagagcgac ggtgttcacg gcgaagttcg tctgcagcgg cgtgcgcttg
     2221 tagaggttgt tcaggatgac gagggccggc gcgtccttct tgagcttgat ctcgagccgc
     2281 acctcctcgc ccgccgaggc gttgttgatg tcggcgatgc cggtgagctc gcccgagtcc
     2341 accagctcct tgatcttcat cagaaccgtg ctggggctga cctggtacgg cagcttcgac
     2401 accacgatca tgtcgttgcg cttgccctcc tcgatctcgg ccacggcccg cagacggatc
     2461 gagccccggc cggtgcggta cgcgtcgagg atcccctggc gccccatcat cagcgcgccg
     2521 gtggggaagt ccgggccctt gatgaacgcc atcaggtcgt cgggcgtggc gtccgggtgg
     2581 tcgatcaggt ggacggtggc atcgatgacc tcgcccaggt tgtggggcgg gatgttggtg
     2641 gccatgccga cggcgatgcc ctggctgccg ttgaccagca ggttcgggaa gcgggccggg
     2701 agcagcgacg gctcctggaa ctcgccggag tagttgtcga cgaagtcgac ggtgtcctcg
     2761 tcgatgccgt cgagcatggc cagcgcgatc tcggccaggc ggcactccgt gtagcgggcc
     2821 gcggccggcg ggtcgtcggg cgtgccgaag ttgcccttcg ggtggatcag ggggtggcgc
     2881 agcgagaagc tctggcccat gcgcaccagc gcgtcgtaga tggccgagtc gccgtggggg
     2941 tggtacttgc ccatgacgtc gccggtgacc cgggcgcact tgatggtggg ccggtcgggg
     3001 cgggccccga ggtcgtgcat gccccagagg atccggcggt ggaccggctt gaggccgtcc
     3061 cgggcgtccg ggagggcgcg ggagacgatg accgacatcg agtagtcgag gaaggagcgc
     3121 tccatctcct cctggatctc gatgggctcg aactcctctt cgaagagggt ctcgtcccgg
     3181 ccgtcggggg gtggggacgg tgtcatatgt ccaggaacct cacgtccttg gcattggtct
     3241 ggatgaagtg cttgcggctc tcgacgtcgt cgcccatgag gaccgagagc acctcgtcgg
     3301 cgatggccgc ctgctcgacg gacaccttca gcagggtgcg cttcgtgggg tcgatcgtgg
     3361 tctcctggag ctcttcccag tccatctcgc ccaggccctt caggcgctgg aactccttct
     3421 tgtgggtggg gttctcggcc aggaaggcgg ccttggccgc gtcgtccttc aagtagatct
     3481 tgtccttgcc cacgatcgtg gagaacagcg ggggctgggc gatgtagacg tgaccgtgct
     3541 cgaccaggtc ccgcatctgg cggaagaaga aggtcagcaa cagggtgcgg atgtggctgc
     3601 cgtcgacgtc ggcgtcacag aggatgatga ccttgccgta gcggatcttc tcggcgtcga
     3661 actcctcgcc caggccggcg ccgatggcgg agatcagggc ctggatctcg ttgttcttga
     3721 gcatcttgtc gatgcgggcc cgctcgacgt tcaggatctt gccccggatg ggcagcacgg
     3781 cctggatgcg ggggtcgcgg gcctgggtgg ccgggccacc ggcggagtcg ccctcgacga
     3841 tgaacagctc ggtctcgtgc atgttccggg attggcagtc ccggagcttg tcgggcatgc
     3901 ccgccccctc gagggccgac ttgcgccggg tggcgttgcg ggcgtcccgg gccgcggtgc
     3961 gggcggtggc tgcggcgatc gacttcttga cgatcttgtt ggcctcggtg gggtgctcct
     4021 cgaggaagtc gcccagcttc tcgttggtgg cccgctcgac cagcgagcgc atggggacgt
     4081 tgcccagctt gcctttggtc tgaccctcga actgggggtc gcgcaggcgg accgagatga
     4141 tggcggtgag gccctcgcgg atgtcctcgc cgaggaggtt ggcgtccttc tccttcagca
     4201 ggcccttgcc ccgggcgtac ttgttgacca cgttggtgag gcccttcttg aacccctcct
     4261 cgtgcatccc gccctcgatg gtgttgatgc cgttggcga
//
LOCUS       DASTFV010000205         6832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_776203, whole genome shotgun sequence.
ACCESSION   DASTFV010000205 DASTFV010000000
VERSION     DASTFV010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776203"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1922)
                     /locus_tag="VFP02_03885"
     CDS             complement(<1..1922)
                     /locus_tag="VFP02_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET9542193.1"
                     /translation="MTALDEPATRHPLAPEARPDAPTSIRGVSTAEAVDDFCLANVSR
                     AIDDREINLQKQSRVYFQISGAGHEALLLGLARHLRPGHDWFFPYYRDLALVLGLGVS
                     PYEVLLQAVGSADDPASGGRQMPAHWGHAERHIVTQSSPTGTQCLPAVGCAEAARYLS
                     RRPNLPGPGAHGDELTYVSLGEGACSEGEFWESLNTACTLHLPVLFVVADNGYAISVP
                     TADQAPAPVSDMVRGFAGLEIHRFDGTDYWKVRKRGAKAIAHVRAGVGPALLHVDVTR
                     PYSHSSADTQSKYRTEDELALEAAHDPMARMAETLVSAGALTPDEVAGIGESARALVA
                     EAAQAALAAARPDPTTVLANVRAPDRLVGRDDPGDGEPVSLGDALNRALHEAMADDER
                     IRVFGEDVADAREAVLAQLEGKGGVFGTTHGLQREFGQARCYNTPLAEANIIGRAVGQ
                     AIRGLRPVPEIQFFDYIWPATQQLKSEAATIRWRSNGAFTCPMVVRVPIGGYLTGGAI
                     WHSQCGESIFAHVPGLAVAFPSRAADAVGLLRAALAGEDPVLFCEHKHLLRQPYAKDP
                     YPGPDHLVPLGQGVIRRPGRHLTIVTWGATVQKSVEAADAIAADGGPEVEVIDLRTIS
                     PWDQELVAESVARTGRV"
     gene            complement(1919..3139)
                     /locus_tag="VFP02_03890"
     CDS             complement(1919..3139)
                     /locus_tag="VFP02_03890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET9542194.1"
                     /translation="MAEPGITEVTMPQMGETVTEGTITAWLKAPGDPIAVDESLLEIS
                     TDKVDSEIPSPVAGVVAELLVPEGDTVPIGQLLARITTDGAAQTPDVDEPVAEDEEPA
                     STPRREAEPLAPSAASEEPAERRRGEVTTFLSPVVRRLVDEHDLDPATIDGSGREGRI
                     TRDDVLALVEAAPPAPDRPGVRRVAFTAIRRTIGRNLVTSLATAAHTLVVVEVDFARV
                     EPVKERTGLTWLPFIARAVIDAISDFPLLNAHVTAEGLDVYDGVHLGVAVDLDFEGLV
                     VPVVRDANDLRLAALAAALKESAAAAREHRLAADDYAGGTFTLTNAGRYGTLLTAPII
                     NQPQAAILSTDGVKIRPVAVPLTHGGHGLAFHPVGNLALSFDHRAFDGAYASAFAAQV
                     RDTLERRDWEDEAR"
     gene            3268..3648
                     /locus_tag="VFP02_03895"
     CDS             3268..3648
                     /locus_tag="VFP02_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542195.1"
                     /translation="MPDPVVPTLNLAVLRGHLSRAPEEVALASGDLLVRYEVTVPRAD
                     VRADTVPVVAVSPPAHARGLDAGTEVVVVGRTRRRFWSTGGATRSQVEVLARAVVPAR
                     RRAGATRALGQALATLEAFAHQEE"
     gene            3653..4708
                     /locus_tag="VFP02_03900"
     CDS             3653..4708
                     /locus_tag="VFP02_03900"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HET9542196.1"
                     /translation="MTDLASLLGDEADSLLAHESKGIPAADLHLPGPDYISRVLEQSD
                     RSPQVLRSLQSLFDHGRLGGSGYVSILPVDQGIEHSGAASFAPNPKYFDPSNICELAV
                     EGGCNGVASTFGVLGAVSRRYAHRIPFIAKLNHAEMLTYPNDADQVMYGSVQQAYDLG
                     AAGVGATIYYGSDRATRQIQEVSEAFQEAHELGLFTVLWAYLRNSAFKVDDVDYHLAA
                     DLTGQANHMAVTIEADIVKQKQPENNGGYNAIAGYGKTHKLVYDELTSDHPIDLTRWQ
                     VANCYMGRIGLINSGGASAGADDLAQAVRTAVINKRAGGLGLISGRKAFQRPTAEGVE
                     LLNAIQDVFLDDAVTIA"
     gene            4789..6663
                     /locus_tag="VFP02_03905"
     CDS             4789..6663
                     /locus_tag="VFP02_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HET9542197.1"
                     /translation="MADTAARDHTVETLDRVIIRFAGDSGDGMQLTGDRFTSASALWG
                     NDLSTLPDFPAEIRAPAGTLAGVSAFQVHISDHEITTPGDMPNVLVCMNPAALKAELG
                     VLEPGGTLILNTDAFTERNLAKAGYDSNPLEGDSLKGYTVYEVPMTTITKESVADLGV
                     KPRDAERSKNFFALGLLSWMYTRPEAPTLDWIAQKFAGREQVIAANTAAFKAGHAFGE
                     TAELFDHPYEVRPAAKAPGTYTNITGNTALAWGLVAAGQLAKLPVFLGSYPITPASDI
                     LHELSKHKNFGVRTLQAEDEIAAITSAIGAAFGGHLGVTTTSGPGLALKAEAMGLAVS
                     LELPLIIIDVQRGGPSTGLPTKTEQADLLMGLYGRSSESPLPIVAPKSPSDCFDTAIE
                     AARIALKYRTPVLLLSDGYLANGAEPWLLPDVDSLPDISVPFATEANGTDGDGNPIYL
                     PYLRNEDMARPWAVPGTPGLEHRIGGLEKEDGTGNVSYDPANHEHMVHLRAERIEHIA
                     GDIPLVEVEGADDAEILVIGWGSTWGAIDGAVDRVNAAGRRAAKAHLTHLHPFPKNLG
                     EVLKRHPKVLVPEMNLGQLSLLLRGHFLVDAQSVTKMKGLPFTSGELERAILAELEKD
                     "
BASE COUNT         1019 a         2464 c         2386 g          963 t
ORIGIN      
        1 acccggcccg tccgggcgac ggactcggcc accagctcct ggtcccacgg actgatggtg
       61 cgcaggtcga tgacctccac ctcgggtccg ccgtcagcgg cgatggcgtc ggccgcctcg
      121 acggacttct gcacggtcgc gccccaggtg acgatcgtca ggtgtcgacc gggccggcgg
      181 atcacgccct gaccgagcgg gaccaggtgg tccggccccg ggtaggggtc tttggcgtag
      241 ggctggcgca gcaggtgctt gtgctcgcag aagaggacgg ggtcctcacc ggcgagcgcc
      301 gcccgtagga ggcccacggc gtcggcggcc cgggagggga aggcgacggc gaggcccggg
      361 acgtgcgcga agatcgactc gccgcactgg gagtgccaga tcgctccgcc ggtgaggtaa
      421 ccgccgatcg gcacccgtac gaccatcggg caggtgaacg ccccgttgga ccgccagcgg
      481 atcgtcgccg cctcgctctt gagctgctgg gtggcgggcc agatgtagtc gaagaactgg
      541 atctcgggca ctgggcgcag gccccggatg gcctggccga cggcccgacc gatgatgttg
      601 gcctcggcca gcggggtgtt gtagcagcgg gcctggccga actcccgctg caggccgtgg
      661 gtggtgccga acacacctcc cttgccctcg agctgggcca ggaccgcttc ccgggcgtcg
      721 gccacgtcct cgccgaagac ccggatgcgc tcgtcgtcgg ccatcgcctc gtgcagcgcc
      781 cggttgagcg cgtcgcccag gctcacgggc tcaccgtcac ccgggtcgtc gcgccccacc
      841 agccgatcgg gcgccctgac gttggccagg accgtggtcg ggtcgggtcg ggccgcggcc
      901 agcgccgctt gggccgcctc ggcaaccaga gccctggccg actcgccgat gccggccacc
      961 tcgtcggggg tcagggcccc ggccgagacg agggtctcgg ccatgcgggc catggggtcg
     1021 tgggccgctt cgagggccaa ttcgtcctcg gtccggtact tggactgggt gtccgccgac
     1081 gagtgggagt acggccgggt cacgtcgacg tggaggaggg cggggccgac accggcgcgt
     1141 acgtgggcga tggctttggc cccccgcttg cgcaccttcc agtagtcggt gccgtcgaag
     1201 cggtggatct cgaggccggc gaagccccgg accatgtccg acaccggtgc cggggcctgg
     1261 tcggccgtgg gcaccgagat ggcgtaaccg ttgtcggcga cgacgaagag caccggcagg
     1321 tggagggtgc acgcggtgtt caggctctcc cagaactcgc cctcggagca ggccccctca
     1381 cccagcgaga cgtacgtcag ctcgtcgccg tgggcccccg ggccggggag gttggggcgg
     1441 cgagagagat aacgggcggc ctcggcgcag cccaccgcgg gcaggcactg ggtcccggtc
     1501 gggctggact gggtgacgat gtggcgctcc gcgtggcccc agtgcgccgg catctggcgg
     1561 ccgccggaag ccgggtcgtc ggccgagccg acggcctgga gcaggacctc gtaaggcgag
     1621 acgccgaggc cgaggacgag ggccaggtcc cggtagtagg ggaagaacca gtcgtgaccg
     1681 ggtcggaggt gacgggccag accgaggagc agggcctcgt ggcccgcacc cgagatctgg
     1741 aagtagaccc ggctctgctt ctggaggttg atctcccgat cgtcgatggc ccgggacacg
     1801 ttggccaggc agaagtcgtc gacggcctcg gccgtggaca cgccccggat ggacgtcggg
     1861 gcgtcgggtc gggcctcggg ggccagggga tggcgggtcg ccggctcgtc gagggcggtc
     1921 atcgggcttc gtcctcccag tcacgtcgtt ccagggtgtc ccggacctgc gcggcgaacg
     1981 ccgatgcata ggccccgtcg aacgcccggt ggtcgaagga gagagccagg ttcccaacgg
     2041 gatggaaggc caggccgtgg ccgccgtgcg tcagcgggac ggcgaccggc cggatcttca
     2101 cgccgtcggt ggacaggatc gccgcctggg gctggttgat gatcggggcc gtgagcaggg
     2161 tcccgtagcg cccggcgttg gtcagcgtga acgtcccgcc ggcgtagtcg tcggcggcca
     2221 ggcggtgctc ccgggccgcc gccgcgctct ccttcaaggc ggcggcgagc gccgcgaggc
     2281 ggaggtcgtt ggcgtcccgg acgacgggca cgaccaggcc ttcgaagtcg aggtcgacgg
     2341 cgacgccgag gtggacgccg tcgtacacgt cgaggccctc cgcggtgacg tgggcgttga
     2401 ggagcgggaa gtcggatatg gcatcgatga ccgcccgcgc gatgaagggg agccaggtga
     2461 ggccggtccg ctccttgacc ggttcgaccc gggcgaagtc gacctcgacg acgaccagcg
     2521 tgtgagctgc cgtggccagc gaggtgacca ggttccgacc gatggtccgc cggatggccg
     2581 tgaaggcgac ccggcgcacg ccgggccggt ccggtgccgg gggcgccgcc tcgaccaatg
     2641 ccagcacgtc gtcccgggtg atccgcccct cccgccccga tccgtcgatc gtcgccggat
     2701 ccagatcgtg ctcgtccacc aaccgacgga cgacgggcga caagaacgtc gtcacctcgc
     2761 cccgcctccg ctccgccggc tcctccgatg cggccgacgg tgccagcggc tctgcttcgc
     2821 ggcgcggcgt cgacgccggc tcctcgtcct cggctaccgg ctcgtcgacg tcgggggtct
     2881 gagccgcacc gtcggtggtg atgcgagcga ggagctggcc gatggggacg gtgtcgccct
     2941 cggggaccag cagctcggcg accaccccgg cgacgggaga cgggatctcc gagtcgacct
     3001 tgtcggtcga gatctcgagc agcgactcgt ccaccgcgat ggggtccccg ggcgccttca
     3061 accaggccgt gatcgtgccc tcggtgacgg tctcgcccat ctggggcatg gtcacctcgg
     3121 tgatccctgg ttcggccata gcgggtcccc caggtcccga cggtcctgtc tcggccaccg
     3181 taactgtcac tacccctgcc taggttggat caggtcgctt cccccgaccc gtcatcacca
     3241 cgcgtccgcg tggaggagga agctgtcatg cccgatcccg tcgtcccgac cctgaacctg
     3301 gccgtgctgc ggggccatct gtcccgggcc cccgaggagg tggccctggc ctcgggcgac
     3361 ctgctcgtgc gctacgaggt caccgtgccc cgcgccgacg tgcgggccga caccgtgccc
     3421 gtcgtcgccg tcagcccgcc ggcccacgcc cgtggcctcg acgccgggac cgaggtcgtc
     3481 gtcgtcggcc gcacccgccg ccgcttctgg tcgaccgggg gtgccacccg ctcccaggtc
     3541 gaggtgctgg cgcgcgccgt cgtccccgcc cgccgtcggg ccggcgccac tcgggccctg
     3601 gggcaggcgc tcgctaccct ggaggcgttc gcacaccaag aggagtgact ccgtgaccga
     3661 tctcgcgagc ctgctgggtg acgaggccga ttccctgctc gcccacgagt ccaagggcat
     3721 ccccgccgcc gacctgcacc tgccgggccc cgactacatc tcgcgggtgc tggagcagag
     3781 cgaccgctcg ccccaggtcc tgcgcagcct gcagtcgctg ttcgaccacg ggcgcctggg
     3841 cggctccggc tacgtgtcca tcctcccggt ggaccagggc atcgagcact ccggcgcggc
     3901 atcgttcgca ccgaacccca agtacttcga cccgagcaac atctgcgagc tggcggtcga
     3961 gggcggctgc aacggcgtgg cctcgacctt cggcgtgctc ggcgcggtgt cccggcgcta
     4021 cgcgcacagg atcccgttca tcgccaagct caaccacgcc gagatgctca cgtacccgaa
     4081 cgacgccgac caggtcatgt acggctcggt ccagcaggcc tacgacctcg gcgccgccgg
     4141 cgtcggcgcc accatctact acggctccga ccgcgccacc cgtcagatcc aggaggtctc
     4201 cgaggccttc caggaggccc acgagctggg cctgttcacc gtcctctggg cgtacctgcg
     4261 caacagcgcc ttcaaggtcg acgacgtgga ctaccacttg gcggccgatc tcaccggcca
     4321 ggccaaccac atggcggtca ccatcgaggc cgacatcgtc aagcagaagc agccggagaa
     4381 caacggcggc tacaacgcca tcgccggcta cggcaagacc cacaagctgg tgtacgacga
     4441 gctgaccagc gaccacccca tcgacctgac ccgctggcag gtggccaact gctacatggg
     4501 ccgcatcggc ctgatcaaca gtggcggggc ctccgccggc gccgacgacc tggcccaggc
     4561 cgtgcgcacc gcggtcatca acaagcgggc cggcggcctc ggcctcatca gcgggcgcaa
     4621 ggcgttccag cgaccgacgg ccgagggcgt cgagctgctc aacgccatcc aggacgtctt
     4681 cctcgacgac gcggtgacca tcgcgtgatc acacccgcgg ccatgcaccc ggatgggggt
     4741 gccgcccggt agggtcgaca gccggaccag agagctcaaa ggagagacgt ggccgacacc
     4801 gctgcccgcg atcacaccgt cgagaccctc gatcgggtga tcatccgatt tgccggtgac
     4861 tccggcgacg ggatgcagct cacgggggac cgcttcacca gcgccagtgc cctctggggc
     4921 aacgacctga gcaccttgcc ggacttcccg gccgagatcc gcgcccccgc cgggacgctg
     4981 gccggggtgt ctgcgttcca ggtccacatc tccgatcacg agatcaccac gccgggcgac
     5041 atgcccaacg tgctggtgtg catgaacccg gccgcgctga aggccgagct cggcgtgctc
     5101 gagcccggcg ggacgttgat cctcaacacc gacgcgttca ccgagcgcaa cctggccaag
     5161 gccgggtacg actcgaaccc cctcgagggc gactcgctca agggctacac ggtgtacgag
     5221 gtcccgatga ccacgatcac caaggagtcg gtggccgatc tgggggtcaa gccccgggac
     5281 gccgagcggt ccaagaactt cttcgccctg ggcctgttga gctggatgta cacccggccc
     5341 gaagcgccca ccctcgactg gatcgcccag aagttcgccg gtcgcgagca ggtcatcgcc
     5401 gccaacaccg cggcgttcaa ggccggccac gccttcggcg agacggctga gctgttcgac
     5461 cacccctacg aggtccgacc cgcggccaag gcccccggga cctacaccaa catcaccggc
     5521 aacaccgccc tggcctgggg cctggtggcc gccggccagc tggccaagct gccggtgttc
     5581 ctcggctcgt accccatcac cccggcgtcg gacatcctcc acgagctgtc caagcacaag
     5641 aacttcgggg tccgcaccct gcaggccgag gacgagatcg cggccatcac cagcgccatc
     5701 ggcgcggcgt tcggcggtca cctcggggtg accaccacca gtgggccggg cctggccctc
     5761 aaggccgagg ccatgggcct ggccgtgagc ctggagctgc cgctcatcat catcgacgtg
     5821 cagcggggcg ggccctccac gggcttgccc accaagaccg agcaggccga cctgctgatg
     5881 ggcctctacg ggcgctcgtc ggagtcgccc ctgccgatcg tggcccccaa gagcccatcg
     5941 gactgcttcg acaccgccat cgaagcggcc cgcatcgccc tcaagtaccg gacaccggtc
     6001 ctgctgctgt ccgacggcta cctcgccaac ggggccgagc cctggctgct gcccgatgtc
     6061 gactcgctgc ccgacatcag cgtgcccttc gccaccgagg ccaacggcac cgatggtgac
     6121 ggcaacccga tctacctgcc ctacctccgc aacgaggaca tggcccggcc ttgggccgtt
     6181 cccggcacgc ccggtctcga gcaccgcatc ggtggcctcg agaaggagga cggcaccggc
     6241 aacgtctcct acgacccggc caaccacgag cacatggtcc acctgcgggc cgagcgcatc
     6301 gagcacatcg ccggcgacat cccactggtc gaggtcgaag gggccgacga cgccgagatc
     6361 ctggtcatcg gctggggctc gacctggggc gccatcgacg gcgccgtgga ccgggtcaac
     6421 gccgccggcc gacgggccgc caaggcccac ctgacccacc tccacccgtt ccccaagaac
     6481 ctgggcgagg tgttgaagcg ccatccgaag gtgctggtcc cggagatgaa cctgggccag
     6541 ctgtcgctgc tgctgcgggg ccacttcctg gtcgacgccc agagcgtcac gaagatgaag
     6601 ggcctgccgt tcacctctgg tgagctggag cgggccatcc tggccgagct ggagaaggac
     6661 tgatcgcctg atgaccgaca ccgacgtccc cgtcacgacc aaggccgact ggtcgtcgga
     6721 ccaggaggtc cgctggtgcc cgggctgtgg ggactactcg atcctcaccg ccgtccagat
     6781 gctgatgccc gaactggggg tgcgtcggga gaacacggtc ttcgtgtccg gc
//
LOCUS       DASTFV010000206         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_778997, whole genome shotgun sequence.
ACCESSION   DASTFV010000206 DASTFV010000000
VERSION     DASTFV010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778997"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VFP02_03910"
     CDS             complement(<1..1034)
                     /locus_tag="VFP02_03910"
                     /EC_number="1.1.1.306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050607 - mycothiol-dependent formaldehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010127 - mycothiol-dependent
                     detoxification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HET9542198.1"
                     /translation="MGQQVRGVVSRAKGEPVTIETIEVPDPGPGEAVVAVKACGVCHT
                     DLHYREGAINDEFPFLLGHEAAGVVESVGDGVDDLEPGDYVILAWRAPCGTCRSCVRG
                     KPWYCFESKNAAQPMTLDGVPLSNALGIGAFADKTLVHAKQAVKVDPAAPPEAAGLIG
                     CGVMAGFGAAVNTGEVRRGETVAVFGCGGVGNAAILGAAVAGARQIIAVDLDDRKLAW
                     AKGFGATDTVNASVGDPVEAIQALTGGLGVDVAIEAIGLPQTFVQAFNARDLAGRLVL
                     VGVPSPDAKVELPMIEIFGRGGSTKSSWYGDCLPTRDFPLLIDMHLSGRIDLGLFVSE
                     TIPLDGVEEA"
     gene            1084..1716
                     /locus_tag="VFP02_03915"
     CDS             1084..1716
                     /locus_tag="VFP02_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_1061 family FMN-dependent PPOX-type
                     flavoprotein"
                     /protein_id="HET9542199.1"
                     /translation="MPTPADSVIRTVDELREVIAPPSDAVTMKLGDGLDEFARDFIAE
                     CPFLVLATSAADGRGDASPKGDEAGFVLVEDDHTLVIPDRPGNHLAFGLTNILENPHV
                     GLLFVIPGTTETLRVNGRAELTRDEDLLEQLGARGKAAVLGIRVHVDEVFFHCAKAFI
                     RSHLWKHETWPPKRKVSFGAMFAAKMDRAADEDLVAVIDDLIETDYAENL"
     gene            1716..1919
                     /locus_tag="VFP02_03920"
     CDS             1716..1919
                     /locus_tag="VFP02_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542200.1"
                     /translation="MILAFLLLAFAAVLAAGAAYEWHIKRHLPHDLREVGTEGLPSDA
                     SIRSGELGRGAASQYFRNLPPGY"
     gene            complement(2093..>2275)
                     /locus_tag="VFP02_03925"
     CDS             complement(2093..>2275)
                     /locus_tag="VFP02_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542201.1"
                     /translation="ILVTRIFINDLEGQAEGAGLLSAFAVSGNQMTKASILLIAAGAA
                     VSLIGSGIAVSRYLKV"
BASE COUNT          350 a          848 c          744 g          333 t
ORIGIN      
        1 aacgcctcct cgaccccgtc cagcgggatg gtctcgctca cgaacaggcc caggtcgatc
       61 cggccgctca ggtgcatgtc gatgagcagc gggaagtccc gagtgggcag gcagtcgccg
      121 taccagctcg acttcgtgga cccgccccga ccgaagatct cgatcatcgg cagctcgacc
      181 ttggcgtcgg gtgacggcac gccgaccagg acgagccggc cggccaggtc tcgggcgttg
      241 aaggcctgga cgaaggtctg gggcaggccg atggcctcga tggccacgtc cacgccgaga
      301 ccgccagtga gggcctgaat ggcttcgacc gggtcaccca ccgacgcgtt caccgtgtcg
      361 gtggcgccga agcccttggc ccatgccagc ttccggtcgt cgaggtcgac ggcgatgatc
      421 tggcgggccc cggccaccgc ggcgccgagg atggcggcgt tgcccacccc accgcagccg
      481 aagacggcaa ccgtctcgcc ccgtcgcacc tcgccggtgt tcacggccgc tccgaaaccg
      541 gccatgaccc cgcacccgat gagaccagca gcctccggcg gggccgccgg gtcgaccttc
      601 acggcctgct tggcgtggac gagggtcttg tcggcgaagg ccccgatgcc cagggcgttg
      661 ctcaggggca cgccgtcgag cgtcatcggc tgggccgcgt tcttggactc gaagcagtac
      721 cagggcttcc cccggacgca ggaccggcac gttccgcacg gagcccgcca ggccaggatc
      781 acgtagtcac cgggctcgag gtcatcgacc ccgtccccga ccgactccac cacgcccgcc
      841 gcctcatggc cgagcaggaa cgggaactcg tcgttgatgg ccccctccct gtagtgcaga
      901 tcggtgtgac agaccccaca cgccttcacg gcgaccacag cctcgcccgg ccccgggtcg
      961 gggacctcga tggtctcgat ggtgaccggc tcgcccttgg cgcgggacac gactccccgg
     1021 acctgctgac ccatgtctga ctctcctcca ctcgcgactg cgccggatcg taggctgcaa
     1081 cccgtgccca ctcccgccga ctcggtgatc cggaccgtgg acgagttgcg ggaggtcatc
     1141 gccccaccca gcgacgcggt gacgatgaag ctgggcgacg gcctcgacga gttcgcccgg
     1201 gacttcatcg ccgaatgccc gttcctcgtg ctggcgacct cggcggcgga cgggaggggc
     1261 gacgcctcac ccaagggcga cgaggccggc ttcgtgctgg tggaggacga ccacaccctg
     1321 gtcatccccg accggcccgg caaccacctg gccttcgggc tgaccaacat cctcgagaac
     1381 ccccacgtgg ggctgctgtt cgtcatcccg ggcaccaccg agacgctgcg ggtcaacggc
     1441 cgggccgagc tgacccggga cgaggacctg ctcgagcagc tcggcgcccg gggcaaggcc
     1501 gccgtgctcg ggatccgggt ccacgtggat gaggtcttct tccactgcgc caaagccttc
     1561 atccgctccc atctctggaa gcatgagacc tggcccccca agaggaaggt cagcttcggc
     1621 gccatgttcg cggccaagat ggaccgcgcc gcggacgagg acctggtggc cgtcatcgac
     1681 gacctcatcg agaccgacta cgccgaaaac ctctgatgat cttggccttc ctgctcctcg
     1741 ccttcgccgc cgtgctcgcg gccggcgccg cctacgaatg gcacatcaag cggcacctgc
     1801 cccacgacct gcgcgaggtc ggcacggagg gcctgccctc cgacgccagc atccgctccg
     1861 gcgagctggg ccgtggtgcg gccagccagt acttccgaaa cctccccccc ggctactgac
     1921 ccagctccgg ctcctgccac tcgggcaaga cgtcgacggc accaccctcg tcctcgaaga
     1981 ccggtgccgc cgccggcctc gtcgccggca tcaccgccgt cgccctccgg gccccgttcc
     2041 tcgtcgtcgt cctcagcgcc gccggcgtga cggcggcgat tcgagcgctg gtctagacct
     2101 tgaggtagcg gctgacggcg atgccggagc cgatgaggct gacggcggca ccggcagcga
     2161 tgagcaggat cgacgccttg gtcatctggt tgccggacac ggcgaaggcg gacagcagtc
     2221 cggcgccctc ggcctggccc tccaggtcgt tgatgaagat ccgggtgacc aggat
//
LOCUS       DASTFV010000207         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_787689, whole genome shotgun sequence.
ACCESSION   DASTFV010000207 DASTFV010000000
VERSION     DASTFV010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787689"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1279)
                     /locus_tag="VFP02_03930"
     CDS             complement(236..1279)
                     /locus_tag="VFP02_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET9542202.1"
                     /translation="MTSVLTPLVASTAAGSENFASFHTPVWAWVALIGAITAMLIIDL
                     LLVHKTAHVISIKEAAIESAVWISIGLAFGLVILAWQGGQAGAEYYAGFLIEKSLSVD
                     NVFVWAVIFSFFAVPKQYQFRVLFWGIFGALVLRGIFIFAGVSLIEQFDWILYVFGAF
                     LLYTAWKIARHDESEVVDYEKSFTMRTVRRLVPSTPEYDGQKLFTVQNGKRLATPLFA
                     ILILIEATDVVFAVDSVPAILAVSRETFIVFSSNAFAILGLRSLYFLLGGMQGKFRYL
                     NVGLGVILAFVGMKMLLIGDPFEIHVPTVVSLGVIFAVLTAAIVASLRADTRDRAAGI
                     LPPKDAVVDVDEE"
     gene            1477..>2736
                     /locus_tag="VFP02_03935"
     CDS             1477..>2736
                     /locus_tag="VFP02_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9542203.1"
                     /translation="MDTTTGSVPGPAGGLTRGQENRALVALALPVLLISMDATILGFA
                     VPRLSEDLEPTSAELLWIIDIYSFVLSGLLITMGNVGDRIGRRRLLLYGAAAFSVASV
                     AAAFAPTPLALIGARALLGVAGATLMPSTLSLIRNIFMDDRRRQFAVAVWATMFSVGA
                     VVGPIIGGLLLEHYWWGSVFLVGVPVTTALLLIGPRVIPESRDPDPGPFDILSSALSM
                     AAILPLVYGMKIVAERGWATLVAPSLVIGLVAGWAFVRRQRVLPVPMIDLSLFAVPKF
                     RVAITGNLLTCVGFAGSTFFVTQYLQLVLGLSPTRAALQLLPAATAGVISTMSAPAMT
                     RRFGTFAVITTGLATGAVGFLLLTQLTVGSSLVPVTVALVIIEAGLCAAITVAIDAII
                     SSVPPEKAGAGASVSETANELGVALGTA"
BASE COUNT          410 a          967 c          933 g          426 t
ORIGIN      
        1 cgtcgtcacc accacgggtg gcgcatccgg ccagggtgac caggccgatc ccgccgagga
       61 gctgcagggc ccgacggcga ccgaccagga ccggcaggtc gacggccagg cccccgtcgt
      121 gggcctcgtg atcgtggact tcgttctcgt gcatcggagg agcacaccag gcccacccgc
      181 gcgactcccg cagagccggc tgagaggttc ctggcaagtc agggcgacgg ggtgctcatt
      241 cctcgtcgac gtccacgacg gcgtccttcg ggggcaggat ccccgccgcc cggtcccgcg
      301 tgtcggcccg gagcgaggcc acgatggccg cggtcaggac ggcgaagatg accccgagcg
      361 agacgaccgt gggcacgtgg atctcgaagg gatcaccgat gagcagcatc ttcatcccga
      421 cgaaggccag gatcacgccg aggccgacgt tgaggtagcg gaacttgccc tgcatgccgc
      481 ccagcaggaa gtagagcgag cgcaggccca ggatggcgaa ggcgttcgag gagaagacga
      541 tgaaggtctc ccggctgacg gccaggatcg cgggcaccga gtcgacggcg aagaccacgt
      601 cggtggcttc gatgaggatc aggatggcga agagcggcgt ggccagccgc ttgccgttct
      661 ggacggtgaa cagcttctgg ccgtcgtact ccggcgtgga gggcaccagg cgccggaccg
      721 tccgcatggt gaagctcttc tcgtagtcca cgacctcgct ctcgtcgtgg cgggcgatct
      781 tccacgcggt gtagagcagg aacgcgccga agacgtagag gatccagtcg aactgctcga
      841 tgagcgagac cccggcaaag atgaatatcc cccggaggac gagcgcgccg aagatgcccc
      901 agaagaggac ccggaactgg tactgcttcg gcacggcgaa gaacgagaag atcacggccc
      961 agacgaagac gttgtcgacc gacaggctct tctcgatcag gaacccggcg tagtactccg
     1021 ccccggcctg acccccctgc caggcgagga tgaccaggcc gaaggccagg ccgatcgaga
     1081 tccacaccgc cgactcgatg gccgcctcct tgatggagat gacgtgggcg gtcttgtgga
     1141 ccagcaggag gtcgatgatc agcatcgccg tgatggcccc gatgagggcg acccacgccc
     1201 agacgggggt gtggaagctg gcgaagttct cgctgcccgc cgcggtgctg gccaccaggg
     1261 gagtgaggac gctggtcatg gggtctcctc ggtcagacgg ggtcgtctgc cgaaggtctc
     1321 tcccacccgc cgggaggcgg gccgctcgcc cgggcgctga ggccgtactg acgaaacgag
     1381 cgtgacgccg gtccgccggg gcggtccgag gccggggata ctccccttca gcttgcgggg
     1441 ccgagtctac gccccgcgga tgccagagtg accgggatgg acacgacgac ggggagcgtc
     1501 cccggaccgg cgggcgggct gacccggggc caggagaacc gtgccctcgt cgcgctcgcc
     1561 ctgcccgtcc tgctcatctc gatggacgcc accatcctcg gcttcgccgt gccccggttg
     1621 agcgaggacc tcgagccgac cagtgccgag ctgctctgga tcatcgacat ctactccttc
     1681 gtgctcagcg gcctgctcat caccatgggc aacgtcgggg accgcatcgg gcggcggcgg
     1741 ctcctcctct acggggccgc cgcgttcagc gtggcctccg tggccgccgc cttcgccccc
     1801 acgccgctgg ccctgatcgg ggcccgggcc ctgctgggcg tggccggggc gacgctcatg
     1861 ccctcgaccc tctcgctgat ccgcaacatc ttcatggacg accggcgccg ccagttcgcc
     1921 gtcgccgtct gggccacgat gttctcggtc ggcgccgtgg tcggcccgat catcggtggc
     1981 ctcctgctgg agcactattg gtggggctcg gtcttcctcg tgggggtgcc cgtcaccacg
     2041 gccctgctcc tgatcggccc ccgggtgatc cccgagtccc gcgaccccga cccgggaccc
     2101 ttcgacatcc tcagctcggc cctgtccatg gccgccatcc tcccgttggt ctacgggatg
     2161 aagatcgtgg ccgagcgggg ctgggcgacg ctcgtggccc cgtccctggt catcggactg
     2221 gtcgcgggct gggccttcgt gcgccgtcag cgcgtcctgc cggtgccgat gatcgacctc
     2281 tcactgttcg ccgtgcccaa gttccgggtg gccatcaccg gaaacctgct gacctgcgtc
     2341 ggcttcgccg ggtccacgtt cttcgtcacc cagtacctcc agctcgttct gggcctctcc
     2401 cccacccggg ccgccctgca gctcctgccg gcggcgactg ccggcgtgat ctcgacgatg
     2461 tccgctccgg cgatgacccg acggttcggc accttcgccg tgatcacgac cggactggcg
     2521 accggagccg tcggcttcct cctcctgacc cagctcaccg tgggcagctc cctggtcccg
     2581 gtcaccgtcg ccctggtgat catcgaggcc gggctctgtg cggccatcac cgtggccatc
     2641 gacgccatca tcagctcagt cccccccgag aaggcggggg ccggggcctc ggtgtcagag
     2701 accgccaacg agctgggcgt ggccctcggc accgcc
//
LOCUS       DASTFV010000208         5388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_788746, whole genome shotgun sequence.
ACCESSION   DASTFV010000208 DASTFV010000000
VERSION     DASTFV010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788746"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VFP02_03940"
     CDS             <1..670
                     /locus_tag="VFP02_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HET9542204.1"
                     /translation="DGQDHLALDTGGGPLARLWSTALSGLVDIGYVKATGTSVQINLP
                     KTALKPEVSFEWKVPQWSNTIERDRARTYFQAYAAACALHFAEKALAEVRAGHTKTWE
                     SFKVPDNAIGCGFTEAVRGVLSHHMVIRDGKIANYHPYPPTPWNANPRDVYGTPGPYE
                     DAVMGTQIFEENDLDHFKGIDIMRTVRSFDPCLPCGVHMYLGDGKTLERTHSPTGVHD
                     GVDA"
     gene            667..1257
                     /locus_tag="VFP02_03945"
     CDS             667..1257
                     /locus_tag="VFP02_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET9542205.1"
                     /translation="MTDAADGAEGAAELRAAGDRIEHLLDRLQADLEPGAWLAVEEVV
                     RRVTDLYGAGLERTLDLADEVLGDEPAGEQLFERLFTDDLVASLLIVHGLHPDELARR
                     VEEALDRVRPYLASHGGGVELLGLDEEGGILRLRLLGSCDGCPSSSATLRDAVENAIH
                     EAAPEIVTIDTQGGVEPDLPGPVAVPLGRKPERQPA"
     gene            1289..1936
                     /locus_tag="VFP02_03950"
     CDS             1289..1936
                     /locus_tag="VFP02_03950"
                     /inference="COORDINATES: protein motif:HMM:NF040271.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HET9542206.1"
                     /translation="MTPGPGALMILDRIRQASSQLPEEERCEMCAVVIPPEHQHVVNL
                     ESRGLLCTCRACYLLFVDRGAAGGKFRSVPDRHLSLTGFGMSQGEWDALQIPVSVAFF
                     FHNSVMDKVVAFYPSPAGATESLLALDAWEHLVIANPVLVSLEPDVEALLVRRADGAS
                     DSFLVPIDACYELVGLLRTRWRGFDGGQEAHEAIDGFFAALATRSTVVPPPEGQS"
     gene            1933..2562
                     /locus_tag="VFP02_03955"
     CDS             1933..2562
                     /locus_tag="VFP02_03955"
                     /inference="COORDINATES: protein motif:HMM:NF039986.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HET9542207.1"
                     /translation="MSLSFEVVDARPEPYAAVPTMMFRLRAEETSGDAVHALALRAQL
                     RIEPQRRHYSADEEDRLYEQFGPTPQWGESLRPFLWTHVATMLPGFTGTTEFDLPVEC
                     TYDFEVTAARFLHALGDGEIPLVFLFSGTQFSHGEAGFNALPVAWNLEATYRLPVRVW
                     RDMMDLYYPNSGWMRLPRDTMDRLGRFKADRALATWDQAVEALLKEADG"
     gene            2559..3998
                     /locus_tag="VFP02_03960"
     CDS             2559..3998
                     /locus_tag="VFP02_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542208.1"
                     /translation="MTVTDTASTATAVAAATSVFDRATTIADAVLYEGYVLYPYRASA
                     GKNQVRWQFGVLTPKPFSEGTGSDPWAMQTDCVVDPGDHPVVQLRLRCLQVQSRTVEA
                     LVDGDFRPVEALEVGGELHTSWDEAVEREFDLVDIALDPVHGDWRPHIDAVFDLPAGR
                     EAEPLRDPDGSVMGQVVRERLAVRAAVEVTPEWLDGPTPLVRLRVRIENRMPWNVPDA
                     PRDEVVLHSLVAVHTLLAVTGGRFVSLLDPPEFARAVTESCENVGTYPVLAGTGSDPD
                     DGAIVLSSPIILYDHPEVAPESPGALFDSTEIDEILALRILTLTDDEKREARATDARV
                     SEIFDRVDAMPPEMFDRLHGAVRYLEGAPSAPPTAGLPGGLEAESELAPWWDPGADAA
                     FDPATDTVWIGGTAVGRGAKVRLRPRPGADAHDMFLAGRTATVEAVLHDVDDEVHVAV
                     VLDDDPGADLFAWQGRFNYFRPDELEVLP"
     gene            3995..4477
                     /locus_tag="VFP02_03965"
     CDS             3995..4477
                     /locus_tag="VFP02_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HET9542209.1"
                     /translation="MSRTLVAGIGNIFFGDDAFGVEVAGRLAGRAMPEGVRVEDFGIR
                     SVHLAYELLDGYDTLVLVDALATGETPGTVSVFEPSLDDLPAATMDAHTMDPATVLAT
                     LAGLGGEVGRVLIVGCEPEGIDEGMGLSPPVAAAIDIAAETVMDLVGATQVASPTLEG
                     "
     gene            4622..4894
                     /locus_tag="VFP02_03970"
     CDS             4622..4894
                     /locus_tag="VFP02_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HET9542210.1"
                     /translation="MCLGIPGQIVELAGGHEHLAKVEVAGVRRNINIGLLEDEPLEEG
                     DWVLIHVGFAMSKIDEVEAARAMEGLALMGQAYEDEVQAVAESRIR"
     gene            4891..>5388
                     /gene="hypD"
                     /locus_tag="VFP02_03975"
     CDS             4891..>5388
                     /gene="hypD"
                     /locus_tag="VFP02_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HET9542211.1"
                     /translation="MRFVDEYRDPVAAGRLLGVIEDLTGDDHFKFMEVCGGHTHTIYR
                     HGIEHVLPDGVELVHGPGCPVCVIPMGRIDDAIAVAETPGLIFTSFGDMMRVPGGNGT
                     LLEAKARGADVRMVYSPLDALRIAVENPERKVVFFAIGFETTAPSTALTLLRARDDGI
                     TNFSVF"
BASE COUNT          769 a         1929 c         1882 g          808 t
ORIGIN      
        1 cgacggccag gaccatctcg ccctcgacac gggcggcggt ccccttgccc gcctctggtc
       61 gaccgcgctc tccgggctcg tcgacatcgg gtacgtcaag gccacgggga ccagcgtcca
      121 gatcaacctg cccaagacgg ccctcaagcc cgaggtcagc ttcgagtgga aggtccccca
      181 gtggagcaac accatcgagc gggaccgggc ccggacctac ttccaggcct acgccgcggc
      241 ctgtgcgctg cacttcgccg aaaaggcgct ggccgaggtc cgcgccggcc acacgaagac
      301 gtgggagtcc ttcaaggtgc ccgacaacgc catcggttgc gggttcaccg aagccgtcag
      361 gggcgtgctc tcgcaccaca tggtcatccg ggacgggaag atcgccaact accaccccta
      421 ccccccgacg ccgtggaacg ccaatccccg ggacgtctac gggacgccgg gcccctacga
      481 ggacgcggtc atgggaaccc agatcttcga ggagaacgac ctcgatcact tcaagggcat
      541 cgacatcatg cgcacggtgc ggagcttcga cccctgcctg ccctgcgggg tccacatgta
      601 cctcggcgat ggcaagaccc tggagcggac gcactcgccg accggcgtcc atgacggtgt
      661 cgacgcgtga cggacgccgc ggacggcgcg gagggcgcag ctgagctgcg ggccgcgggg
      721 gaccgcatcg agcacctgct cgaccgcctg caggcggatc tcgagcccgg cgcgtggctg
      781 gcggtcgagg aggtcgtgcg ccgcgtgacc gacctgtatg gcgccggcct cgagcgaacg
      841 ctcgatctgg ccgacgaggt cctcggtgac gagcccgccg gcgaacagct cttcgagcgg
      901 ctgttcaccg acgacctcgt cgccagcctc ctgatcgtgc acgggctcca ccccgacgag
      961 ctcgcccggc gggtggagga ggccctggac cgcgtccggc cctacctcgc ctcgcacggc
     1021 gggggcgtcg agctcctggg cctcgacgag gaaggtggga tcctgcgcct ccgtctcctg
     1081 ggcagctgcg acggttgccc gtcctcgtcg gccacgctcc gcgatgccgt ggagaacgcg
     1141 atccacgagg ccgcgccgga gatcgtgacc atcgacacgc aggggggcgt cgagcccgac
     1201 ctccccggac cggtggcggt gcccctgggg cgcaagcccg agcggcaacc ggcctgagcc
     1261 gatgagcgcc aacgggcacg aaccacccgt gacgcccggc ccgggcgcgc tcatgatcct
     1321 cgatcggatc cggcaggcct cgtcccagct cccggaggag gagcgatgcg agatgtgtgc
     1381 ggtggtgatc ccccccgagc accaacacgt ggtcaacctc gagagccggg gcctcctctg
     1441 cacctgccgg gcctgctacc tgctcttcgt cgaccgcggc gcggcgggtg ggaagttccg
     1501 ttcggtccct gaccgccacc tgtccctgac cggtttcggc atgagccagg gcgagtggga
     1561 cgcgctccag atcccggtca gcgtcgcctt cttcttccac aactcggtca tggacaaggt
     1621 cgtggccttc taccccagcc cggcgggggc gaccgagtcc ctcctggccc tggacgcgtg
     1681 ggagcacttg gtgatcgcca acccggtcct cgtctccctg gagccggacg tcgaggcgct
     1741 cctggtccgt cgggccgacg gggccagcga ctccttcctc gtgcccatcg acgcctgcta
     1801 cgagctggtc ggcctgctgc ggacccggtg gcggggcttc gacggcggcc aggaggccca
     1861 cgaggccatc gacgggttct ttgccgccct ggccacccgc tcgacggtgg tcccgccccc
     1921 ggagggccaa tcgtgagcct gtccttcgag gtggtggacg cccgcccgga gccctatgcg
     1981 gcggtgccga cgatgatgtt ccgtcttcgg gccgaggaga cctcgggcga cgccgtgcac
     2041 gccctcgcgc ttcgggccca gctccgcatc gagccgcagc gccgtcacta ctcggccgac
     2101 gaggaggacc gtctctacga gcagttcggc cccacgccgc aatgggggga gtcgctccga
     2161 ccgttcctgt ggacccacgt ggcgaccatg ctgccgggct tcacggggac gaccgagttc
     2221 gacctgccgg tcgaatgcac ctacgacttc gaggtcacgg cggcacggtt cctgcatgcg
     2281 ctcggcgacg gcgagatccc cctggtcttc ctgttcagcg ggacccagtt ctcccacggg
     2341 gaggcgggct tcaacgccct gccggtggcc tggaacctgg aggcgaccta ccggctcccg
     2401 gtacgggtct ggcgggacat gatggacctc tactacccga acagcgggtg gatgcgactg
     2461 ccgcgggaca cgatggaccg gctgggccgg ttcaaggccg accgggcctt ggccacctgg
     2521 gatcaggccg tggaggcgtt gctcaaagag gcggacgggt gacggtgacc gacaccgcct
     2581 ccaccgccac agcggtggcc gcggcgacct ccgtcttcga ccgggcgacc acgatcgccg
     2641 acgccgtcct ctacgagggg tacgtgctct acccctaccg ggcgtcggcc ggcaagaacc
     2701 aggtgcggtg gcagttcggg gtgctcacgc cgaagccgtt cagcgaaggc accggctcgg
     2761 acccgtgggc catgcagacc gactgcgtcg tcgaccccgg tgaccacccg gtcgtccagc
     2821 tccggctgcg ctgcctccag gtgcagtccc gcacggtgga ggccctggtc gacggcgact
     2881 tccgtccggt ggaggccctg gaggtcggtg gtgagctgca cacctcctgg gacgaggccg
     2941 tcgagcggga gttcgatctc gtcgacatcg cgctcgaccc ggtccacggc gactggcggc
     3001 cgcacatcga cgcggtcttc gacctcccgg cgggccgaga ggccgagccg ctccgggatc
     3061 cggacggcag cgtcatgggg caggtcgtgc gggagcggct ggcggtccgg gccgccgtcg
     3121 aggtgacgcc ggagtggctg gacgggccca cgcccctggt ccgcctccgg gtccggatcg
     3181 agaaccggat gccttggaac gtgcccgacg cgccgcggga cgaggtcgtc ctgcattcgc
     3241 tcgtggccgt ccacacgctg ctggcggtca ccggtggtcg attcgtcagc ctcctcgacc
     3301 cgcccgagtt cgcccgagcc gtcaccgagt cctgcgagaa cgtcgggacc tatcccgtcc
     3361 tggccgggac ggggagtgac ccggacgacg gcgccatcgt gctgtcctcg ccgatcatcc
     3421 tctacgacca ccccgaggtg gccccggaga gccccggtgc cctgttcgac tccaccgaga
     3481 tcgacgagat cctcgcgctg cgcatcctca ccctcaccga cgacgagaag cgggaggcgc
     3541 gggccaccga cgcccgcgtc tcggagatct tcgatcgagt cgacgccatg ccgcccgaga
     3601 tgttcgatcg tctccacggc gccgtgcgct acctggaggg tgccccgtcc gcccctccga
     3661 cggcggggct cccgggcggc ctggaggccg aatccgagct ggcgccctgg tgggacccgg
     3721 gggccgatgc cgccttcgac ccggcgaccg acaccgtctg gatcggcggg accgcggtcg
     3781 gccggggcgc caaggtccgc ctccggcccc ggcccggcgc cgacgcccat gacatgttcc
     3841 tggccggtcg gacggcgacg gtcgaggcgg tgctccacga cgtggacgac gaggtccacg
     3901 tggccgtcgt gctcgatgac gaccccggcg ccgatctctt cgcgtggcag ggccgtttca
     3961 actacttccg gcccgacgag ctcgaggtcc tcccgtgagc cggaccctgg tcgccggcat
     4021 cggcaacatc ttcttcggtg acgacgcgtt cggcgtcgag gtcgcgggac gcttggcggg
     4081 ccgggcgatg cctgaagggg tgcgggtgga ggacttcggc atccgcagcg tccacctcgc
     4141 ctacgagctc ctcgacggct acgacaccct cgtcctggtc gacgccctcg cgaccggaga
     4201 gacgccgggc acggtgtcgg tcttcgagcc cagcctcgac gacctcccgg ccgcgacgat
     4261 ggacgcccac acgatggacc cggcgaccgt cctcgccacc ctggccggcc tcggcggcga
     4321 ggtcggacgc gtgctcatcg tcggctgcga gccggagggc atcgacgagg gcatgggcct
     4381 gtcgcccccg gtcgccgccg ccatcgacat cgcggccgag acggtcatgg atctcgtcgg
     4441 agcgacgcag gtcgcgtcgc cgaccttgga ggggtgatgg aaacactggg gatcatcttc
     4501 ttggtgctca tcggggcggc cgtgatcgtc gtcgcggtcc tcttcctggc ctcgctgccg
     4561 gacctgtcgc gctacctgcg tgtcaagcgg atgtgatgcc ccgacccgtg gaggtcgcct
     4621 gatgtgcctg gggataccgg gtcagatcgt cgagctcgcc ggcggccacg agcacctggc
     4681 caaggtcgag gtggccggcg tgcgccgcaa catcaacatc ggcctgctcg aggacgagcc
     4741 cctcgaggag ggtgactggg tgctgatcca cgtgggcttc gccatgtcga agatcgacga
     4801 ggtggaggcg gcccgggcca tggagggcct ggcgctgatg ggccaggcct acgaggacga
     4861 ggtccaggcc gtggccgagt cgaggatccg atgaggttcg tcgacgagta ccgggacccg
     4921 gtcgcagccg gacgcctcct gggtgtcatc gaggacctca ccggtgacga ccacttcaag
     4981 ttcatggagg tgtgcggggg tcacacgcac accatctacc gccacgggat cgagcacgtc
     5041 ctgcccgacg gcgtcgagct ggtgcacggc cccgggtgcc cggtctgtgt gatccccatg
     5101 ggccgcatcg acgacgccat cgccgtggcc gagaccccgg ggctgatctt caccagcttc
     5161 ggcgacatga tgcgggtgcc cggcggaaac gggacgctgc tggaggccaa agcccgtggg
     5221 gcggacgtcc gcatggtgta ctcgccgctc gatgcgctgc ggatcgccgt ggagaacccg
     5281 gagcggaagg tcgtcttctt cgccatcggc ttcgagacga ccgcgccctc cacggcgctc
     5341 acccttctgc gggcccgcga cgacgggatc accaacttct cggtgttc
//
LOCUS       DASTFV010000209         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_790557, whole genome shotgun sequence.
ACCESSION   DASTFV010000209 DASTFV010000000
VERSION     DASTFV010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790557"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFP02_03980"
     CDS             <1..396
                     /locus_tag="VFP02_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9542212.1"
                     /translation="SSIAGVAAFPGMATYAATKAGLSQFTEALALDLRGLPVGTTLVE
                     LGPIPTDMLDHVDTYQPTRDSFDRAYRLGLIADVPRERVADEVVRAVERDRAHVRLPK
                     RAVAYPLMTQLPRELTRLTLTGIRHRREP"
     gene            complement(486..1178)
                     /locus_tag="VFP02_03985"
     CDS             complement(486..1178)
                     /locus_tag="VFP02_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET9542213.1"
                     /translation="MADAVSPDRSYRAVAGGLDMPLQEAMLTQRAVRRVLPDPVDDEI
                     VLRCIELALKAPTGSNGQNWEFVIVKDADAKASLAARYRQAWKVYGGIGHRMNKGDES
                     MTKVLRAVEWQVDHFEEIPVLVVACLRGSRVPLLPTPPIAGASYYGSIFPSVQNLLLA
                     ARAMGLGASLITLPLWSTTAARRALGLPISVQPACVVPLGWPQGRYGPTTRRPVGDVV
                     HLDRFGNQPFKA"
     gene            complement(1205..1687)
                     /locus_tag="VFP02_03990"
     CDS             complement(1205..1687)
                     /locus_tag="VFP02_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HET9542214.1"
                     /translation="MNILVTHASKMGGTQGLAEAIAAGLGEGAVVLPVDAVKRLDGYD
                     AVVVGGGLYAGRWTGAGRRFVRRHAKELRERDVWFFSSGPLDDSAHEHPIDPTRSVRR
                     MMEKVGAKGHVTFGGRLPADAKGFPASAMAKEHAGDWRNLDEARAWGQEVAAEMSADA
                     "
     gene            1798..2220
                     /locus_tag="VFP02_03995"
     CDS             1798..2220
                     /locus_tag="VFP02_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HET9542215.1"
                     /translation="MVKIVFCLRRKDGMEVDEFQRYWREEHAPLVQTYAAALGIVRYV
                     QSHTRLGPEADLLRASRGGPEAYDGVAELWFRDTTALIEAASTPEGRAAGAALLEDER
                     RFIDLAHSPIFLAEEHEVVGHAGGGGAPLGVDTAWDGP"
     gene            complement(2273..2524)
                     /locus_tag="VFP02_04000"
     CDS             complement(2273..2524)
                     /locus_tag="VFP02_04000"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HET9542216.1"
                     /translation="MPLFLDVHNIEGGVSADEVAGAHQADLATQDAHGVSYKRYWVDE
                     EAGKIFCLVEAPDAEAAHTVHREAHGLVADEIYEVSEHA"
BASE COUNT          360 a          948 c          954 g          390 t
ORIGIN      
        1 agctcgatag ccggcgtcgc tgccttcccc ggcatggcca cctacgccgc caccaaggcg
       61 gggctctccc agttcaccga ggccctggcc ttggacctcc gggggctgcc cgtcggcacc
      121 acgctggtcg agctcggacc gatccccacc gacatgctgg accacgtcga cacctaccaa
      181 cccacgcgcg actccttcga ccgggcgtac cggctgggcc tgatcgccga tgtcccccgg
      241 gagcgggtgg ccgacgaggt ggtgcgggcg gttgagcggg accgagccca cgtccgactg
      301 cccaagcggg ccgtcgccta ccccctgatg acccagctgc cccgcgagct cacccggctc
      361 accctcaccg gtatccgcca ccgcagagag ccctgacccg gtcatgatcc cgtgccgggg
      421 cgaaccccgc aaccggctgc gcccgccacg ggcggcgccc acttcttcct ggccgaggtc
      481 gaggctcagg ccttgaaggg ctggttcccg aagcggtcga ggtggacgac gtcgccgacg
      541 gggcggcggg tcgtcgggcc atagcgaccc tgaggccagc cgagggggac gacgcaggcg
      601 ggctggaccg agatcggcag gccgagggcc ctgcgagcgg ccgtggtgct ccacaacggc
      661 agggtgatca gcgacgcccc caggcccatg gcccgggcgg ccaagagcag gttctgcacg
      721 ctggggaaga tcgagccgta gtacgaggcg ccggcgatcg gcggcgtcgg cagcagcggc
      781 acgcgcgagc ccctgaggca ggcgacgacc aggacgggga tctcctcgaa gtggtcgacc
      841 tgccactcga cggccctcag gaccttggtc atcgactcgt cgcccttgtt catgcggtga
      901 ccgatgccgc cgtagacctt ccaggcctgt cggtaacggg cggccaggga cgccttcgcg
      961 tccgcatcct tgacgatcac gaactcccag ttctggccgt tgctgccggt gggtgccttc
     1021 agggccagct cgatgcagcg caggacgatc tcgtcgtcga cggggtcggg caggacccgg
     1081 cgcacggccc gctgggtgag catcgcctcc tgcagcggca tgtcgagccc gccggccacg
     1141 gcgcggtagg agcggtcagg ggacacggcg tcggccatgg cgcatcttcg cagcgtgctg
     1201 ccggtcaggc gtcggcggac atctctgccg cgacctcttg gccccaggcc cgggcctcgt
     1261 cgaggttgcg ccagtcaccg gcgtgctcct tggccatggc cgatgccgga aagcccttgg
     1321 cgtcggcggg caggcggccc ccgaaggtga catggccctt cgccccgacc ttctccatca
     1381 tccgccggac cgaccgcgtc ggatcgatcg ggtgctcgtg ggccgagtcg tcgagggggc
     1441 cgctgctgaa gaaccagacg tccctctctc gcagctcctt ggcgtggcgg cggacgaagc
     1501 ggcgaccggc accggtccag cgaccggcat agaggccacc gccgacgacg acggcgtcgt
     1561 aaccgtccag gcgcttgacg gcatcgacgg gcaagaccac tgcgccttcg ccgaggcctg
     1621 ctgcgatcgc ttcggccagc ccctgggtgc cccccatctt gctggcgtgg gtgacgagga
     1681 tgttcatggg agcctccggt ggatcgggtt ctcctgccaa gtcaccatcc tccgccgacg
     1741 ccggcagggc caggggcctt ggtcccgact gcgggccggt cgtgccagag tgcgggcatg
     1801 gtcaagatcg tgttctgcct gcggcgcaag gacggaatgg aggtggacga gttccagcgc
     1861 tactggcggg aggagcacgc cccgctggtc cagacctacg ccgcggccct cggcatcgtc
     1921 cggtacgtcc agtcccacac ccgcctcggc cccgaggccg acctgctgcg ggcctcccgg
     1981 ggtgggcccg aggcctacga cggggtggcc gaactgtggt tccgcgacac cacggcactg
     2041 atcgaggccg cctcgacccc cgagggcagg gccgccgggg ccgccctgct ggaggacgag
     2101 cggcggttca tcgacctggc ccactcgcca atcttcctgg ccgaggagca cgaggtcgtc
     2161 ggtcacgccg gcggcggggg tgcgcccctc ggcgtcgaca ccgcctggga cgggccgtag
     2221 cccggccggg cgggtctggc tgccgggtcc cggcagccag cccgcgctcg ggctacgcgt
     2281 gctcgctcac ctcgtagatc tcgtcggcca cgaggccgtg ggcctcgcgg tggacggtgt
     2341 gggcggcttc ggcgtcgggg gcctcgacga ggcagaagat cttccccgcc tcctcgtcca
     2401 cccagtagcg cttgtagctg acgccgtgcg cgtcctgggt ggcgaggtcg gcctggtggg
     2461 cgccggcgac ctcgtcggcc gacaccccgc cttcgatgtt gtggacgtcg agaaacagtg
     2521 gcatgaacgg gttctcctgt tcgtaggccc gaccgggacg gccggggggt gttcgcggtg
     2581 ggcagcggac cacgggggtg tttgacccgg cgtttgacac tccgctccat cgggagaagg
     2641 ttctcccggg ca
//
LOCUS       DASTFV010000210         4626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_792074, whole genome shotgun sequence.
ACCESSION   DASTFV010000210 DASTFV010000000
VERSION     DASTFV010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792074"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="VFP02_04005"
     CDS             complement(<1..1048)
                     /locus_tag="VFP02_04005"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HET9542217.1"
                     /translation="MNFGAAISEHPLTTQAAGEVVGEVLEQVGEAPDLAVLFVSAAHL
                     GLMAEIAETVRTALRPRALIGVTASSIVGGGREVEDRPAISLWAGNVGPVEPVRLETQ
                     RLNGGWALTGLPAETDAVTPRVLLLLADPFSIPTDGMLDRLRAKAPTVQVVGGMASTG
                     RAPGGNRLVLDDAVFDNGAVGVLLDSGVVSGTVVSQGCRPVGQAYVVTRATDNVIHDL
                     AGVPALDRLRDIVAGLPPGDRALVQEGLHVGRVIDEQKDSFGRGDFLISNVMGADPEA
                     GDITIGATAEVGSTVQFQVRDADSADEDLRHLLAGQQGRAALVFTCNGRGRNLFDAPD
                     HDARLVHDQVGQGAV"
     gene            complement(1045..1434)
                     /locus_tag="VFP02_04010"
     CDS             complement(1045..1434)
                     /locus_tag="VFP02_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542218.1"
                     /translation="MEILSALFVDNVDLRPAEGGASRIDLTGVQFSAVAPSPLPLTWA
                     PHLSVIIRCAPDEPGAGVLEVIYERDGEQLARNVQPLEVEPGKFTQRLVRGEIDFEEY
                     GTIVARCRVDQGPVLNVPYTMLAPPES"
     gene            1579..1947
                     /locus_tag="VFP02_04015"
     CDS             1579..1947
                     /locus_tag="VFP02_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9542219.1"
                     /translation="MSQYMLSVHHDPVDDARMAAMTPDEMQATFKAVDAVNAEMRSAD
                     AWVFGGGLHDKDATTTVRLKDGDTLVTDGPFAETKEYLGGFWIIEAADLDQALDWAKK
                     AAVACGGDVEVRPFQDEPDA"
     gene            1959..3173
                     /locus_tag="VFP02_04020"
     CDS             1959..3173
                     /locus_tag="VFP02_04020"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9542220.1"
                     /translation="MTQDVDLESVFREERGAVVATLIRLFGDIDVAEEAVQEAFLVAA
                     EQWPVKGVPPNPGGWILTTAKNRSIDRFRRESTRHHRHIQASQLHDPDEPPEETGPVR
                     DDRLRLMFTCCHPSLATEAQVALTLRLLGGLETGAIAKAFLVPEPTMAQRLVRAKRKI
                     RDAKIPYRVPGDAELPDRLRPVLAVVYLIFNEGYTASEGDELGREDLAAEAIRLARLL
                     TDLMPDEPEVHGLLALLLLTESRRPARTAADGSIVLLGDQDRERWDPDLAAEGQAIVA
                     ACIRRGLPGPYQLQAAINAVHSEARSATDTDWSQILANYDHLLTLNPSPVVALNRAVA
                     LAEVEGPAAGLVEVAGLDLDGYHLYHATRADLLVRLDRWEEAATAYDRALEVVTNETE
                     RRFLEGRRATLA"
     gene            complement(3367..4464)
                     /locus_tag="VFP02_04025"
     CDS             complement(3367..4464)
                     /locus_tag="VFP02_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9542221.1"
                     /translation="MHSRIRAAASVLPDGRRGPTELEVVNGRLAAVRPLVEGGLPEWT
                     LVPGFVDLQVNGIGAVDVAADIDEHWRKLDRSLISQGVTAWYPTLGSAPLDSYAAALA
                     RIEGTRGRPGVRPAILGTHMEGPFLGDRHGAHPDGFVAPPDPEWFAGLDPVVRLMTLG
                     PEAPGAVDLVQDLVARDVVVALGHTAATPERVTAAVDAGATLFTHLFNASGTVGARSP
                     GPVGVALTDDRLAVSVIADLVHVDPVTLAVVFRAKPADRVVLVTDAVAWQSAWAKDQG
                     ITMVDGAPRLPDGTIAGSALTMDLAVRNLVQVVGVDLVDAVRAASTNPARVMGHADRG
                     VLTVGARADVVALDADLEVAQVWIAGQPMLD"
BASE COUNT          703 a         1691 c         1619 g          613 t
ORIGIN      
        1 ccaccgcccc ctggccgacc tgatcgtgga ccaggcgggc gtcgtgatcg ggagcgtcga
       61 agaggttgcg accgcgaccg ttgcaggtga agacgagggc ggcccgcccc tgctggccgg
      121 ccagcaggtg gcgcaggtcc tcatccgcgc tgtccgcatc acggacctgg aactgcacgg
      181 tcgaaccgac ctcggcggtg gcgccgatgg tgatgtcgcc ggcctccgga tcggcaccca
      241 tgacgttgct gatcaggaag tcgcctcgcc cgaaggagtc cttctgctcg tcgatgaccc
      301 ggcccacgtg gagcccctcc tgcacgaggg cccggtcgcc gggggggagg ccggccacga
      361 tgtcgcgcag gcggtccaag gccggcacgc cggcgaggtc gtggatgacg ttgtcggtgg
      421 cccgagtgac cacgtacgcc tggccgacgg gacggcagcc ctgggagacc acggtgcccg
      481 acacgacgcc cgagtcgagg agcacgccga cggcgccgtt gtcgaacacg gcgtcgtcga
      541 ggaccagacg gttcccaccc ggggcccgac cggtggaggc catgcccccg accacctgca
      601 cggtcggcgc cttcgcccgg agacggtcga gcatgccgtc ggtcgggatg ctgaaggggt
      661 ccgccaacag caggagcacc cggggggtga cggcgtcggt ctcggcgggc aggccggtga
      721 gcgcccaccc accgttgaga cgctgggtct ccaagcggac cggctcgacg gggccgacgt
      781 tgccggccca gaggctgatg gccggccggt cttctacctc ccgcccgccg ccgacgatcg
      841 atgaggccgt caccccgatg agggctcggg ggcgcagggc ggtgcgcacc gtctcggcga
      901 tctcggccat caggcccaga tgggccgcgg acacgaagag cacggccaga tcgggggcct
      961 cgcccacctg ctcgaggact tcgcccacca cctcgcccgc ggcctgggtg gtcagcgggt
     1021 gttcggagat ggcggcgccg aagttcatga ctctggcggc gccagcatgg tgtagggcac
     1081 gttgagcacc ggaccctggt cgacgcggca cctggccacg atcgtcccgt actcctcgaa
     1141 gtcgatctca ccccgcacga ggcgttgggt gaacttgccc ggctccacct cgaggggctg
     1201 cacgttgcgg gccagctgct cgccgtcccg ctcgtagatg acctcgagca cgcccgctcc
     1261 cggctcgtcg ggggcacacc ggatgatcac cgagaggtgt ggcgcccagg tgagggggag
     1321 gggtgagggg gccacggccg agaactgaac cccggtcagg tcgatgcggg aggccccgcc
     1381 ctcggctggc cgcaggtcga cgttgtccac gaacagggcg gagaggatct ccacgtcggg
     1441 gacggtagcg agtcggtcgc gcggcgcggc cttgtcggtt cgggagagac ttcgcccacc
     1501 ctgtcgattc cggagccggt cgttcgtaga cagggcagga accggtgccg tgccggatcc
     1561 ccagcgaagg agacaccgat gagccagtac atgctttccg tgcaccacga tcccgtcgac
     1621 gacgcccgca tggcggcgat gacgcccgac gagatgcagg cgaccttcaa ggccgtcgac
     1681 gccgtcaacg ccgagatgag gtcggccgac gcgtgggtct tcggcggcgg cctccacgac
     1741 aaggacgcca cgacgacggt ccggctgaag gacggcgaca ccctcgtcac cgacggcccg
     1801 ttcgccgaga ccaaggagta tctgggcggc ttctggatca tcgaggccgc ggacctggac
     1861 caggccctcg actgggccaa gaaggccgcc gtggcctgtg gcggcgacgt cgaggtccgc
     1921 cccttccagg acgagcccga cgcctgaggc ccgggccgat gacccaggac gtcgacctcg
     1981 aaagcgtctt ccgcgaggag cggggcgcgg tcgtggccac cctgatccgc ctcttcggtg
     2041 acatcgacgt cgccgaggag gcggtccagg aggcgttcct ggtcgccgcc gagcagtggc
     2101 cggtcaaggg cgtacccccg aatcccgggg gttggatcct gaccacggcc aagaaccgtt
     2161 cgatcgaccg cttccggcgc gagtccaccc gccaccaccg tcacatccag gccagccagc
     2221 tccatgaccc cgacgagccc cccgaggaga ccggacccgt gcgcgacgac cgcctccggc
     2281 tgatgttcac ctgctgccac ccgtctctgg ccaccgaggc ccaggtggcc ctcaccctgc
     2341 gcctcctcgg tggcctggag accggagcca tcgccaaggc ctttctggtc ccggagccga
     2401 cgatggccca gcgcctggtc cgagccaagc gcaagatccg ggacgccaag atcccgtacc
     2461 gggtgcccgg cgacgccgaa ctgccggacc gcctgcgtcc ggtgctggcc gtcgtctacc
     2521 tgatcttcaa cgagggctac accgccagcg agggcgacga gctgggccgg gaggacctgg
     2581 ccgccgaggc catccgcctc gcccggctgc tgaccgacct gatgcccgac gagcccgagg
     2641 tccacgggct gctcgccttg ctcctgctca ccgagtcccg ccgacccgcc cgtaccgcgg
     2701 ccgacgggtc gatcgtcctc ctcggtgacc aggaccgcga gcgctgggac cctgacctgg
     2761 ccgccgaggg tcaggccatc gtcgccgcct gcatccgccg cggcctgccc ggcccctacc
     2821 agctccaagc cgccatcaac gccgtgcaca gcgaggcccg gtcggcgacc gacaccgact
     2881 ggtcccagat cctggccaac tacgaccatc tcctcacgct gaacccgagc cccgtcgtgg
     2941 ctctgaaccg ggccgtcgca ctggccgaag tcgagggccc ggcggcgggc ctggtcgagg
     3001 tggcgggcct cgacctcgac ggctaccacc tgtaccacgc cacccgcgcc gacctcctgg
     3061 tccgcctcga ccgctgggag gaggccgcca ctgcctacga ccgggccctc gaggtggtta
     3121 ccaacgagac cgaacgccgt ttcctcgagg gccgacgagc gacgttggcc tgaccggctg
     3181 ggcaccgacg caggtagggg tccggctccc cgcctccgcc tgcatcaacc aactttcggc
     3241 agaactcgac ctctacggcc ggtgatgccg aaggttcgac gccgctcggc aaccctcggc
     3301 aggactcgac gcctatggcc ggtgatgacg acgtcggcgc caggccaacg tccagcccgc
     3361 ttggcttcag tcgagcatgg gttggccggc gatccagacc tgggccacct cgaggtcggc
     3421 gtcgagggcg acgacatcgg cgcgggcccc gacggtgagg acgccccgat cggcatggcc
     3481 catcacccgc gccgggttgg tggacgcggc gcggacggcg tccacgaggt ccacgccgac
     3541 gacctgcacc aggttgcgca cggccaggtc catcgtgagc gcgctgcccg cgatcgtccc
     3601 gtccggcagt cggggcgccc cgtcgaccat cgtgatgccc tgatccttcg cccatgccga
     3661 ctgccaggcc acggcgtcgg tgacgagcac gacccggtcg gcgggcttgg cccggaagac
     3721 gacggcgagg gtgacggggt ccacgtggac caggtcggcg atgacggaga cggcgaggcg
     3781 gtcatcggtg agcgcaacgc cgacggggcc gggggagcga gcccccaccg tcccggaggc
     3841 gttgaacagg tgggtgaaga gcgtggcccc ggcatcgacg gccgccgtga cccgctccgg
     3901 cgtcgccgcc gtgtggccca gcgcgacgac gacgtctcgt gcgaccaggt cctgcacgag
     3961 gtccaccgct ccgggcgcct cggggccgag agtcatcaac cggaccaccg gatcgagccc
     4021 ggcgaaccac tccgggtcag ggggcgcgac gaacccgtcc ggatgggccc cgtgacgatc
     4081 gcccaggaag gggccctcca tgtgcgtgcc caggatcgcc ggccgaaccc cgggccggcc
     4141 ccttgtgccc tcgatccgag ccaacgcggc ggcgtagctg tcgaggggcg cgctgcccag
     4201 cgtcgggtac cacgccgtca ccccctggct gatgagcgat cggtccagtt tccgccagtg
     4261 ctcgtcgatg tcggcggcca cgtccaccgc cccgatgccg ttgacctgga ggtccacgaa
     4321 ccccggcacc agtgtccact cgggcaatcc accctcgacc agcggacgga ccgcggcgag
     4381 acggccgttg accacctcga gctcggtggg tccacgccgg ccatcgggca ggaccgatgc
     4441 cgcggcgcgg atccgagagt gcatcctcgg acgctaccga caaggcctcg acgagggccc
     4501 gacaccgatg cctccggggg acgcgggtgg gccggtgtac cgtcaaggca tcgtgtccga
     4561 gtcagcgctg cccctttcgc gccgggacga gatcctcgtt gccgccgaag agctgttcgc
     4621 ccagaa
//
LOCUS       DASTFV010000211         3072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_794577, whole genome shotgun sequence.
ACCESSION   DASTFV010000211 DASTFV010000000
VERSION     DASTFV010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794577"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VFP02_04030"
     CDS             complement(<1..461)
                     /locus_tag="VFP02_04030"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET9542222.1"
                     /translation="MSRAVAAGGHRATCEAALRVLDAGGNAFDAIVAAGFASATSEPL
                     MTGLGGGGFCLTRTAAGEATLFDFFVDTPGRGLDPMPEPHFFPVAVRYPGVDQVFNTG
                     MGSAAVPGTVNGYLAIHERLGRLPLAEVVAPAIELAREGVVLDEFHGSVSDL"
     gene            complement(458..1627)
                     /locus_tag="VFP02_04035"
     CDS             complement(458..1627)
                     /locus_tag="VFP02_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HET9542223.1"
                     /translation="MARRRVTDASSSLEALLTANEVVVTCGSGGVGKTTSAAALGAMA
                     ASRLGGRVLVLTVDPAKRLANALGLEQFGNVESEVPRSAFTDAGVQPRGQLFAAMLDT
                     KASWDDLVRLHAPDAATRDAILANPLYQNITGKFVQSHDYIAMERLYEIHSSGRYDLI
                     IVDTPPTRNAIDFLEAPDRMADFFGSRLLRWLTAPARSRVLNYASKPFYSIADRILGS
                     QFLEDIGEFFLLFQTMYDGFVERSHAVARLLEDHGTTFVVVSTLEPAPAQEAEFFVEA
                     LTERKLHLGALVFNKVLPTDLFSAEAAKVADRLDRDAAPVAEAVADRVPDVKQATRVL
                     NEVAESFLNYRLVASREAEEAARLGAKAEVVATVPYLADDIHDLAGLIRIGAHLW"
     gene            complement(1627..2580)
                     /locus_tag="VFP02_04040"
     CDS             complement(1627..2580)
                     /locus_tag="VFP02_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HET9542224.1"
                     /translation="MVAPVDLLDRKLVFVTGKGGVGKSTIASALALVAARRGQKTLAC
                     EFDAKGNLADFFSAAPTAWEPSEVQPNLFAMSMDTEESLKEYLRLQVKIPLVARIGPL
                     ARTLDFVANAAPGVKEILTVGKVVWETKEKHYDLVVVDASATGHVVAQLSAPQAIGEL
                     VKVGLVRDQTAWLSEILDDAATTGAVIVAAPEEMPVNESLELAERIRTETRVDLAATV
                     VNRVLPELFSRDEEAVFAALREPDTVAALEAAAGGPVAPILDAAELAVTLRRTRSVHL
                     DRLREGLRGVPMLYAPELFTRSHGIRATSQLAEALAAELGY"
     gene            complement(2585..2815)
                     /locus_tag="VFP02_04045"
     CDS             complement(2585..2815)
                     /locus_tag="VFP02_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HET9542225.1"
                     /translation="MDVRIGVTYSPKELEVELGEKADPDKLKAEIDTALSGTGALWLT
                     DRRGRQVAVPVDKVAYVEIGSPEDGHRIGFGA"
     gene            2862..>3072
                     /locus_tag="VFP02_04050"
     CDS             2862..>3072
                     /locus_tag="VFP02_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542226.1"
                     /translation="MIRLRPGVRTWNVASSDFNDRLVDLPDGTTVGLYEYGDPSGAPV
                     FAFHGVPSCGAGFDWTDEPARTRGLR"
BASE COUNT          445 a         1053 c         1130 g          444 t
ORIGIN      
        1 aggtcgctga cggagccgtg gaactcgtcg aggacgacgc cctcccgagc cagctcgatg
       61 gccggcgcca ccacctcggc cagcggcagt cggccgagcc gttcgtggat ggccaggtag
      121 ccgttgaccg tgccgggcac ggcggccgag cccatgccgg tgttgaacac ctggtccacg
      181 cccgggtacc ggaccgcgac cgggaagaag tgcggctccg gcatcgggtc gaggccccgg
      241 cccggcgtgt ccacgaagaa gtcgaacagg gtggcctcgc cggccgcggt ccgggtcagg
      301 cagaacccgc ccccgccgag cccggtcatg agcggctcgc tggtcgccga ggcgaagccg
      361 gccgccacga tggcgtcgaa ggcgttgccg ccggcgtcga ggacgcgcag cgcggcctcg
      421 cacgtcgccc ggtgcccgcc ggccgcgacc gcgcggctca ccacaggtgg gcgccgatcc
      481 ggatcaggcc cgccaggtcg tggatgtcgt cggccaggta gggcacggtg gccacgacct
      541 cggccttcgc gccgagccgc gccgcctcct cggcttcgcg ggaggccacc aggcggtagt
      601 tgaggaagct ctcggccacc tcgttgagga cccgggtcgc ctgcttgaca tcggggacgc
      661 ggtcggccac ggcctcggcg accggcgcgg cgtcacgatc caggcgatcg gcgaccttgg
      721 ccgcctcagc cgagaagaga tcggtcggca ggaccttgtt gaacaccagg gccccgaggt
      781 ggagcttgcg ctcggtgagt gcctcgacga agaactcggc ctcctgggcg ggggcgggct
      841 ccagcgtgga cacgacgacg aaggtcgtgc cgtggtcctc gaggaggcgg gccaccgcat
      901 gcgagcgttc gacgaagccg tcgtacatgg tctggaacaa gaggaagaac tccccgatgt
      961 cctccaggaa ctgcgagccg aggatgcggt cggcgatcga gtagaagggc ttcgaggcgt
     1021 agttgaggac gcgggaccgg gcgggagcgg tcagccagcg caacagtcgg ctgccgaaga
     1081 agtcggccat ccggtcgggc gcttcgagga agtcgatggc gttccgggtc ggcggggtgt
     1141 ccacgatgat caggtcgtag cgcccggagg agtggatctc gtagagccgt tccatggcga
     1201 tgtagtcgtg gctctggacg aacttgccgg tgatgttctg gtagagcggg ttggccagga
     1261 tggcgtctcg ggtggcggcg tccggcgcgt ggaggcggac gaggtcgtcc cagctggcct
     1321 tcgtgtcgag catggcggcg aagagctggc ccctcggctg gacgccggcg tcggtgaagg
     1381 ccgaccgcgg cacctccgac tcgacgttgc cgaactgctc caggccgagg gcattggcca
     1441 ggcgcttggc cggatcgacc gtcagcacca gcacccgccc cccgaggcgg gaggcggcca
     1501 tggcccccag ggccgcggca ctcgtcgtct tgcccacgcc gcccgagcca caggtcacga
     1561 cgacctcgtt ggcggtcagc agcgcctcga gcgaggacga cgcatcggtg actcggcgtc
     1621 gcgccatcag taccccagct ccgcggcgag ggcctcggcc aactggctgg tggcccggat
     1681 gccatggctg cgggtgaaga gctctggtgc gtacaacatc ggcacgcccc gcagaccctc
     1741 gcgtaggcga tcgaggtgga cgctgcgggt cctccgcagg gtgaccgcca gctccgccgc
     1801 gtcgaggatg ggagccacgg gcccgcccgc cgcggcctcc agggcggcca cggtgtcggg
     1861 ttcccgcagg gcggcgaaca cggcctcctc gtcgcgggag aacagctcgg gcaggacccg
     1921 gttgacgacg gtggcggcga ggtcgacccg ggtctcggtc cggatccgct cggccagctc
     1981 cagggactcg ttgaccggca tctcctcggg cgcggcgacg atgaccgccc ccgtggtcgc
     2041 ggcgtcgtcg aggatctcgc tcagccacgc cgtctgatcc cggaccagcc ccaccttgac
     2101 cagctcgccg atggcctgcg gggccgagag ctgggccacg acatggccgg tggccgaggc
     2161 gtcgaccacc acgaggtcgt agtgcttctc cttggtctcc cagaccacct tgccgacggt
     2221 gaggatctcc ttgaccccgg gggcggcgtt ggccacgaag tccagcgtgc gggccagggg
     2281 gccgatgcgg gccaccagcg ggatcttgac ctggaggcgc aggtactcct tcagcgactc
     2341 ctccgtgtcc atggacatgg cgaagaggtt gggctggacc tcgctgggct cccaggcggt
     2401 gggcgcggcg gagaagaagt cggccaggtt gcccttggcg tcgaactcgc aagccagggt
     2461 cttctgacct cgccgggcgg ccaccagggc cagcgccgac gcgatggtcg atttgccgac
     2521 accgcccttg ccggtgacga agaccagctt gcggtcgagc agatcgaccg gggcgaccat
     2581 caggtcaggc gccgaacccg atccggtggc cgtcctcggg gcttccgatc tcgacgtacg
     2641 ccaccttgtc caccggcacg gccacctgcc ggccccgccg gtcggtcagc cagagggcgc
     2701 cggtgcccga cagcgcggtg tcgatctccg ccttgagctt gtcggggtcg gccttctcgc
     2761 cgagctccac ctccagctcc ttggggctgt aggtcacgcc gatgcgaacg tccacggatc
     2821 ctcctgtgcg agtgcgggga caggttagga gcgccggtcc catgatccgc ctccgtccgg
     2881 gggtgcgaac atggaacgtg gcttcttccg acttcaacga ccgcctcgtc gacctacccg
     2941 acgggacgac cgtcggcctc tacgagtacg gcgacccgtc gggcgcgccc gtgttcgcgt
     3001 tccacggggt cccgtcctgt ggggccggat tcgactggac cgacgagccg gcgaggaccc
     3061 gaggcctgcg cc
//
LOCUS       DASTFV010000212         3067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_798809, whole genome shotgun sequence.
ACCESSION   DASTFV010000212 DASTFV010000000
VERSION     DASTFV010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798809"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFP02_04055"
     CDS             complement(<1..210)
                     /locus_tag="VFP02_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HET9542227.1"
                     /translation="MSDGAPHPAEFDEPPRPAGRVPPHNLDAEASLLGAMLLSSDAIS
                     AAIQIVTVDDFYKPAHAHVFDAITSL"
     gene            complement(415..861)
                     /gene="rplI"
                     /locus_tag="VFP02_04060"
     CDS             complement(415..861)
                     /gene="rplI"
                     /locus_tag="VFP02_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET9542228.1"
                     /translation="MELILRADVSGLGQRGDIVEVADGYGRNYLMPKGLAIKVTAGAR
                     EQAVSMAKSRAVQDAAEREAAEGVATQLAGRTITIAMRAGDEGKLFGSVTAGDVVDAV
                     AAQASIEIDRHDLVLETAIKEVGTHEVVVRPHPEVQIPLIVEVTPA"
     gene            complement(1089..1340)
                     /gene="rpsR"
                     /locus_tag="VFP02_04065"
                     /pseudo
     CDS             complement(1089..1340)
                     /gene="rpsR"
                     /locus_tag="VFP02_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071887.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            complement(1341..1835)
                     /gene="ssb"
                     /locus_tag="VFP02_04070"
     CDS             complement(1341..1835)
                     /gene="ssb"
                     /locus_tag="VFP02_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET9542229.1"
                     /translation="MAPGNTVTIVGNATREPELRFTASGQAVATFGVAVNRRWQNRNN
                     EWEESTSFFDVTCWAQLAENVAESVPKGGRVVVTGRLDQRSWETQEGDKRSKVEIVAD
                     DVAPSLRWATAQITKNERREGGDGGGFAGGSGGGGGGGSSRPAPSAPAATGGGGYDPD
                     EEPF"
     gene            complement(1852..2199)
                     /gene="rpsF"
                     /locus_tag="VFP02_04075"
     CDS             complement(1852..2199)
                     /gene="rpsF"
                     /locus_tag="VFP02_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET9542230.1"
                     /translation="MTRVYELMVIFDREVDESTIDGLLNQVTSMIGDAGGRVATTDKW
                     GMRKFAYEIDHKDEGYYVVLEIVTEATDLHEVDRFLRLADETVRHKTFRLPDAEATRR
                     GLLGDSVEPAPAG"
     gene            complement(2365..2919)
                     /locus_tag="VFP02_04080"
     CDS             complement(2365..2919)
                     /locus_tag="VFP02_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HET9542231.1"
                     /translation="MLVVVVACADDGGGTATTTAAAPVTVTDPIDLSAEGQQSASGRV
                     TIPGFDEVQVSVTSEAGDVVESCLLLAAAAEQYTQGLMNVVDLGDYAGMLFDFPEGTR
                     DGGFWMRDTPMPLSIAYLDAEGAIVSTADMDPCLDLGDECPGYRPDGPYADTVEVPQG
                     GLAAIGLDGPGARLVQSGDCAANF"
BASE COUNT          405 a         1056 c         1109 g          497 t
ORIGIN      
        1 gagcgaggtg atggcgtcga agacgtgggc gtgggccggc ttgtagaagt cgtcgaccgt
       61 gacgatctgg atggccgccg agatggcatc gctcgacagc agcatggccc cgagcagcga
      121 ggcctcggcg tcgaggttgt gcggcgggac ccggcccgcc ggccgcggcg gctcgtcgaa
      181 ctccgccgga tggggcgcgc cgtcactcac ggacgacccc cccatttccc acgccgtcac
      241 tctgccgcgg cgaaacctgc gccggaaggg gacgccctgt ggatcttcgg gggatctcgc
      301 ggaggttgtc cacagcaagc catcgcgaca tgaagagatg atcgctgagg ggtgtgacag
      361 tcagccgtca tcggcggccg cgatccgacc tcgaccgcgt gggcgtcggc tcgatcaggc
      421 gggggtgacc tcgacgatga gggggatctg gacctcgggg tgcgggcgga ccacgacctc
      481 gtgggtgccc acctccttga tggccgtctc cagcaccaga tcgtggcggt cgatctcgat
      541 cgaggcctgg gcggcgacgg cgtcgaccac gtcaccggcg gtgacggagc cgaagagctt
      601 gccctcgtca ccggcgcgca tggcgatggt gatcgtgcgg ccggccagct gggtggccac
      661 gccttcggcg gcctcccgct cggcggcgtc ctgcacggcc cgtgacttgg ccatggacac
      721 ggcctgctcg cgagcgccgg ccgtgacctt gatggccagg cccttgggca tgaggtagtt
      781 ccggccgtag ccgtcggcca cctcgacgat gtcgccacgc tggccgaggc cggacacgtc
      841 ggcgcggaga atgagctcca tcagccttcc tcagtcgggg cgccgaccgg ctcgacctcg
      901 ggggtggcct ccacgtcggg ggccgcctcg aacgtggcgt cgaccccgtc gagctcggcc
      961 gcgaggtcga gctcctcgtc cacaccgccc gtggggggcg gcgtggccgg cgtcggcatg
     1021 ggcatcgggg cgtcggcgcg gggaccccgc tcgccacggt cccgcgacgt gcgctgggtg
     1081 gtcacccggc tggtgtaggg cagcagcgcc atctcacggg cgttcttgat cgccatggcg
     1141 atctcgctct gctgctgctg ggtgttgccg gtcacccggc gggcgcggat cttggcccgg
     1201 tcgctcatga accgacggag gaggttgacg tccttccagt ccacgtaggg cacctgctcc
     1261 tggagcagga tgctgacctt cttcttgccg cgccgggcgt tgtccttgtt cttggtccgt
     1321 cggacctgct gcttggccat ctagaagggc tcctcgtccg ggtcgtagcc gccgccaccc
     1381 gtggcggccg gcgcgctggg ggcgggtcgg ctgctgccgc ccccaccacc accgccgcta
     1441 ccgccggcga agccgccgcc gtcgccgcct tcgcgacgct cgttcttggt gatctgggcc
     1501 gtggcccacc gcaggctcgg ggcgacgtcg tcggccacga tctcgacctt ggagcgcttg
     1561 tcgccctcct gggtctccca ggaccgctgg tccaggcgcc cggtcacgac gacccgaccc
     1621 cccttgggca cggactcggc cacgttctcg gccagttggg cccagcaggt gacgtcgaag
     1681 aaggaggtcg actcctccca ctcgttgttg cggttctgcc agcgccggtt cacggccacg
     1741 ccgaaggtgg cgacggcctg gccgctggcg gtgaagcgca gttcgggctc gcgggtcgcg
     1801 ttgcccacga tggtgacggt gttgcctggt gccattgctc tgctccttcg atcagccggc
     1861 gggagccggc tccaccgagt ccccgagcag gccgcggcgg gtggcctcgg catcggggag
     1921 acggaaggtc ttgtggcgga cggtctcgtc cgcgaggcgg aggaagcggt cgacctcgtg
     1981 gaggtcggtg gcctcggtca cgatctcgag gacgacgtag tagccctcgt ccttgtggtc
     2041 gatctcgtag gcgaacttgc gcatgcccca cttgtcggtg gtggccacgc gcccaccggc
     2101 gtccccgatc atcgaggtca cctggttgag cagcccgtcg atggtggact cgtcgacctc
     2161 gcggtcgaag atcaccatga gttcatacac ccgcgtcatg cgagcatcac ttcccttcgg
     2221 ttccggcgcc gatgcacctg gagccccggt gggggcaggg tcggtgtctc cccggaggga
     2281 gaccttgtcg gcccacgagg ctaacgggac gggctcgaac ttccctcacg gaccgcagtg
     2341 tcccccggag gtgtgacagc cggatcagaa gttcgcggcg cagtccccgg actggaccag
     2401 acgggcgccg gggccgtcca ggccgatggc ggccagcccg ccctggggga cctcgacggt
     2461 gtcggcgtac ggcccatcgg gccgatagcc cgggcactcg tcgccaaggt cgaggcaggg
     2521 gtccatgtcc gcggtcgaca cgatcgcccc ctcggcgtcg aggtaggcga tggacaacgg
     2581 catcggcgtg tcccgcatcc agaagccgcc gtcccgggtg ccttcgggga agtcgaagag
     2641 catccccgcg tagtcaccca ggtccaccac gttcatcagg ccctgcgtgt actgctcggc
     2701 ggcggcggcc agcagcaggc aggactcgac cacgtcaccc gcctcgctgg tgaccgacac
     2761 ctggacctcg tcgaagcccg ggatcgtgac ccgccccgac gccgactgct gcccctcggc
     2821 ggacaggtcg atgggatcgg tgaccgtgac gggcgcggcg gcggtggtgg tggcggtccc
     2881 acccccgtcg tcggcacagg cgacgacgac gacgagcacg atcagcgttc gtgcgacacg
     2941 agaccacccc atggtggtcc attctggcgg ttggcctcgc gctactccgc gcggccgtag
     3001 aggccgaggg tctcggcgac ctcgtcggag aggtggtagc tctcgtcgtc ggaggggaag
     3061 gtgcccc
//
LOCUS       DASTFV010000213         2776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_800247, whole genome shotgun sequence.
ACCESSION   DASTFV010000213 DASTFV010000000
VERSION     DASTFV010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800247"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1952
                     /gene="purL"
                     /locus_tag="VFP02_04085"
     CDS             <1..1952
                     /gene="purL"
                     /locus_tag="VFP02_04085"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HET9542232.1"
                     /translation="IRIESHNHPSAIEPYQGAATGAGGILRDIFTMGARPIALMDPLR
                     FGPLDDPRSRWIAEGVVAGISGYGNSVGVPTVGGEVVFDETYRGNPLVNVLCLGVMPT
                     DRLVLGQASGEGNLAVLLGSSTGRDGIGGVSVLASSGFSEATDADKRPSVQVGDPFEE
                     KRLIEACLTLLDAGLVVGIQDFGGAGLTCATSETASRAGMGMDVDVSAVRRREPGMEP
                     FEVMTSESQERMLAIVEPGDLDEVMAICARWEVAADVVGKVTTGGRLRILDGWDGEVL
                     ADVPASSLHDAAPLYDRPLTFPDRPADADADVDLVPADCGADLLGLLTDTSWVYSQYD
                     HQLFLNTVVGPGDDATVLRLKHPTTGEDTGRGLALTTDGNHRWCAVDARQGTTMVVAE
                     SLLNLACVGARPLALVNCLNFGDPEHPEVMGQLSDAVDGMSEACRAFGLPVVGGNVSL
                     YNASEGADIDPTPVVGVLGLVDELIAVPPGAGLVAGGRLVLIGPEHDGLGGSEWARAT
                     GRARQGDLPPLDLRLHSAVADVVRALVVDRVVDGVHDVASGGLGLCLAEMAVACGTGF
                     TVDGVADHRGLFSEAPSRVVVCVTPDREAEVLARADAAGVSTRVLGSAGGDRLVVTGL
                     VDVSLAEATNTWRERLPDALDAVAR"
     gene            1981..>2776
                     /locus_tag="VFP02_04090"
     CDS             1981..>2776
                     /locus_tag="VFP02_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HET9542233.1"
                     /translation="MTPPADDVVDAGAVDLEDGKKEACGVVGVYAPGQPVSHLLYLGL
                     YALQHRGQESAGMAVSGGDHVMVVKDMGLVATVFDDRTLGSLSGHLGIGHTRYSTSGT
                     SSWRNAQPVFRDAPAGQFALGHNGNLVNTEALALEAGMLPGMVTSDSDLVAEMIQREL
                     PASTDETSDGRALERALVDVLPKLEGAFSFVLMDESRIIGVRDPNGFRPLCLGRLESG
                     WVLASESPALDVVGAHFVRELDPGEMVVIDATGVRSMKPFDEERLNP"
BASE COUNT          355 a         1014 c          988 g          419 t
ORIGIN      
        1 ccattcgcat cgagtcgcac aaccacccgt cggccatcga gccctaccag ggggcggcca
       61 ccggggccgg ggggatcctg cgcgacatct tcaccatggg cgcccgcccc atcgccctca
      121 tggacccgct gcgcttcgga cccctggacg acccccgttc ccgatggatc gccgaggggg
      181 tcgtggccgg catctcgggc tacgggaact ccgtcggcgt ccccaccgtc ggcggggagg
      241 tggtgttcga cgagacctac aggggcaatc cgctggtgaa cgtcctctgc ctgggggtga
      301 tgcccacgga ccgtctcgtc ctcggtcagg cctccgggga ggggaacctg gccgtgctcc
      361 tcggctcgtc cacgggccgg gacgggatcg gaggggtcag cgtcctcgct tcgtcgggct
      421 tcagcgaggc gaccgacgcc gacaagcggc catcggtcca ggtcggtgat cccttcgagg
      481 agaagcgcct catcgaggcc tgcctcaccc tgctcgacgc gggcctggtg gtcggcatcc
      541 aggacttcgg cggggccggc ctgacctgtg ccaccagcga gaccgcctcc cgggcgggca
      601 tgggcatgga cgtcgacgtg agcgccgtcc gccgtcggga gccgggcatg gagcccttcg
      661 aggtgatgac cagcgagtcc caggagcgga tgctcgccat cgtcgagccc ggggacctcg
      721 acgaggtcat ggccatctgc gcccggtggg aggtggcggc ggacgtcgtg ggcaaggtga
      781 cgacgggggg ccggctgcgg atcctggacg gctgggacgg ggaggtcctg gccgacgtcc
      841 cggcgtcctc cctccacgac gccgccccgc tctatgaccg gccgctgacc ttcccggacc
      901 gtccggcgga cgccgacgcc gatgtcgatc tcgtccctgc cgactgtgga gccgatctgc
      961 tcggcctcct gaccgacacc tcgtgggtgt acagccagta cgaccaccag ctcttcctca
     1021 acaccgtcgt gggccccggc gacgacgcca ccgtgctgcg cctcaagcac cccaccacgg
     1081 gcgaggacac cggccggggc ctggccctga ccaccgacgg gaaccaccgt tggtgcgccg
     1141 tggacgctcg gcagggcacg accatggtcg tggccgagtc cctcctgaac ctggcctgcg
     1201 tcggggcccg gccgctggcg ctggtcaact gcctgaactt cggtgacccc gagcaccccg
     1261 aggtcatggg ccagctgtcc gatgccgtcg acggcatgag cgaggcctgt cgggccttcg
     1321 gcctgcccgt ggtgggcggc aacgtcagcc tctacaacgc ctccgagggc gctgatatcg
     1381 accccacgcc ggtcgtcggg gtcctcggcc tggtcgacga gctgatcgcc gtccccccgg
     1441 gcgcaggcct ggtcgcgggt ggccggctgg tgctcatcgg tccggagcac gatggattgg
     1501 gtggctccga gtgggctcgc gccaccgggc gcgcccggca gggggacctg cccccgctcg
     1561 acctgcggtt gcactccgcc gtagccgatg tcgtgcgggc cctggtcgtg gaccgggtcg
     1621 tcgacggcgt gcacgacgtg gccagcggtg gtctcggcct ctgcctggcc gagatggccg
     1681 tggcctgcgg gacgggcttc accgtcgacg gcgtcgccga ccaccggggc ctcttctccg
     1741 aggcgccgtc gcgggtcgtg gtctgcgtga cccctgatcg ggaggccgag gtgctggccc
     1801 gggccgatgc cgccggcgtg tccacccgcg tgctcggttc ggcgggcggc gaccgcctgg
     1861 tcgtcaccgg cctcgtcgac gtgagcctcg ccgaggccac caacacctgg cgcgagcgcc
     1921 tccccgacgc cctcgacgcc gtcgcccgtt gacgggccgg gcgtcgctag gatcgccgct
     1981 gtgacgcccc ccgccgacga cgtcgtcgac gcgggcgccg tcgatctgga ggacggcaag
     2041 aaggaggcgt gcggggtcgt cggggtctac gcccccggcc agccggtgtc ccacctcctc
     2101 tatctggggc tctacgcgct ccagcaccgg ggccaggaat ccgcggggat ggcggtcagc
     2161 ggtggtgatc acgtgatggt ggtcaaggac atgggcctgg tggccaccgt cttcgacgac
     2221 cggaccctgg gctcgttgtc cggtcacctc ggcatcggcc acacccgcta ctcgacgtcg
     2281 ggcacctcca gctggcgcaa cgcccaaccc gtgttccggg acgcgcccgc cggccagttc
     2341 gccctcggcc acaacggcaa cctggtcaac accgaagccc tggccctcga ggccgggatg
     2401 ttgcccggca tggtcaccag cgacagcgac ctggtggccg agatgatcca gcgggagctg
     2461 cccgcctcga ccgacgagac cagcgacggc cgggccctgg aacgggccct ggtcgacgtg
     2521 ctgcccaagc tggagggcgc cttctccttc gtcctcatgg acgagagtcg catcatcggt
     2581 gtccgcgacc ccaacggctt ccgtcccctg tgcctcggtc ggctcgagtc gggctgggtg
     2641 ctggcctcgg agtccccggc cctcgacgtg gtcggcgccc acttcgtgcg cgagctggac
     2701 ccgggcgaga tggtcgtgat cgacgccacc ggggtccgct ccatgaagcc cttcgacgag
     2761 gaacggctga acccca
//
LOCUS       DASTFV010000214         4932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_802189, whole genome shotgun sequence.
ACCESSION   DASTFV010000214 DASTFV010000000
VERSION     DASTFV010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802189"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="VFP02_04095"
     CDS             <1..1510
                     /locus_tag="VFP02_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015570095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HET9542234.1"
                     /translation="IFSGLMLGMLLAALDQTIVATALPTIVGDLGGLDHLSWVVTSYL
                     LAATVSTPIWGKLGDLLGRKRMYQVAILVFLVGSVLSGLSTTMAQLIAFRFVQGLGGG
                     ALIVLAQAIIADVVSPRERGRYQGYFGALFAASSVAGPLLGGFFTDHLSWRWVFYVNL
                     PLGILALLVTSAVLPATGARARVVIDYLGAGLLAGAVSCIVLVTTWGGTQYPWTSPTI
                     IGLSAAALALVGAFVLAERRAEEPLLPLRLFRGSIFSVSSGVSFVIGVAMFGSISFLP
                     LFLQVVGGVSATNSGLLLVPYMLGMLGASVCAGQIVTRTGKYRVLPVTGTGIAVLGLA
                     LLSTMDSSTTRLQSGVYMTILGIGLGLSMQVLVLATQNSVKVSDLGVATSSVNFFRSV
                     GGSVGVAIFGALFNSRLTAELAGGAGAIQGTITPQAIDALPATDQAVFIDAFATALTD
                     VFLIAVPVMIVAFVLTWFLREIPLMASSGQARALAQEGAVQKTEVAAVALAG"
     gene            complement(1518..2924)
                     /locus_tag="VFP02_04100"
     CDS             complement(1518..2924)
                     /locus_tag="VFP02_04100"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HET9542235.1"
                     /translation="MTEPTPAPAVPPAPPTPVGRILMIVAGALIVLVAGAVVLAGAAL
                     TWAHATQRDDDGYFSTSSERLDTATPVIISDELDLGVSPDDTRWFDAGDLATVRIDVE
                     GVGESPAFVGIGPAADVAAYLDGVAQAQIEDIEVDPFRVEYQFRDGADQADPPTDQDF
                     WVAQGTDGEPLVWDLESGDWVVVVMNADGSAGVSVDAAVAAKVDWLLPLGIGLLIGGF
                     VFLVGGALLVVFGAVGLSRHSAAHPAAVPAVGSTVSSSAPIRLTGHLDDGLSRWLWLV
                     KWVLIIPHVIVLAVLWVGVGVVWIIAWFAIVFTGRYPRSLFGYTVGVMRWTWRVGFYS
                     YAALGTDRYPPFSFEAQPDDPAGFDVDYPERLSRGLVWVKSWLLAIPHLIIIGILAGG
                     GAGAWWGGRRVGPGLIGVLVVIAGVGLLFTSRYARSLFDLIVGLNRWVFRVLAYVLLL
                     RDEYPPFRLDQGGTEPDG"
     gene            3001..3834
                     /locus_tag="VFP02_04105"
     CDS             3001..3834
                     /locus_tag="VFP02_04105"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9542236.1"
                     /translation="MTSLAERLGYDADARLLILNCDDLGSAHAANLAVYEALRDGVAT
                     SASLMVPCPWARDAAQRYLGEDVGVHLTLNAEWDVYRWGPITQAPSLLDGDGGFPRTL
                     EDLWDHGDIDEVRRECRAQVERAILWGFDVSHLDSHMGGLQLKAEFFDVYLELAVDFG
                     LPLRLSGASTEHLIGFPFRELAAAEGVVFPDQFVYVQGVGSRASLQEALFDLRPGVTE
                     VYVHPAVDSAELRASFPDAAARIDDHELVTSDTGIRAMIERSGVQLIGFRELRDLQRA
                     G"
     gene            complement(3846..4697)
                     /gene="mca"
                     /locus_tag="VFP02_04110"
     CDS             complement(3846..4697)
                     /gene="mca"
                     /locus_tag="VFP02_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HET9542237.1"
                     /translation="MADLCLLTVHAHPDDEASKGAPTVARYHDEGVRTVLVTATGGEA
                     GDILNPAMDRPEIRDRIDEVRREELARSAKVIGYDEVVLLGYRDSGMPDSEDNAHPDN
                     FANAPLSEAVGRFVEIIRREKPQVIITYGDDQRGYPHPDHLRVHDISLPAFDAAGDPT
                     AYPEMGEPWEPQKLYYVVWSRARIQATHEKFVELGIESPFGEDWFKRPSQDDQITTSI
                     DIADWYDVRLDSLLAHETQVDPTSPFWFGLPRDVSRTVHPYDDYVLARSRVESELPEA
                     DLFAGLR"
BASE COUNT          701 a         1815 c         1664 g          752 t
ORIGIN      
        1 catcttcagc ggcctcatgc tcggcatgct cctggccgcc ctcgaccaga ccatcgtggc
       61 caccgccctc cccaccatcg tgggcgacct cggcgggctc gaccatcttt cctgggtggt
      121 cacctcctac ctgctggcgg ccacggtctc gacgccgatc tggggcaagc tcggcgacct
      181 cctggggcgc aagcgcatgt accaggtggc catcctggtc ttcctggtcg gctccgtcct
      241 gtccggcctc tcgaccacga tggcccagct catcgccttc cggttcgtgc agggcctcgg
      301 gggcggtgcc ctcatcgtcc tcgcccaggc gatcatcgcc gacgtcgtca gccctcgcga
      361 gcggggccgc taccagggct acttcggcgc cctgttcgcc gcctccagcg tggccggccc
      421 cctcctcggc ggcttcttca ccgaccacct gtcctggcgc tgggtcttct acgtcaacct
      481 ccccctcggc atcctcgccc tcctcgtgac cagtgccgtc ctgccggcga cgggggcccg
      541 ggccagggtg gtcatcgact acctgggcgc cgggctgctg gccggcgcgg tcagctgcat
      601 cgtcctcgtg accacctggg gcggcaccca gtacccgtgg acctccccca cgatcatcgg
      661 cctcagcgcc gccgcactgg ccctcgtcgg tgccttcgtg ctcgcagaac ggcgagccga
      721 ggagccgctc cttcctctcc gcctgttccg agggtccatc ttcagtgtga gcagcggcgt
      781 gagtttcgtc atcggcgtgg ccatgttcgg ctccatcagc ttcctgcccc tgttcctgca
      841 ggtggtgggc ggggtgtcgg ccacgaactc cggcctcctg ctggtcccct acatgttggg
      901 catgctgggc gcctccgtct gcgccggcca gatcgtcacc cggacgggca agtaccgggt
      961 cctgccggta acgggtacgg gcatcgccgt cctgggcctg gccctgctgt ccaccatgga
     1021 cagcagcacg acccgcctcc agtccggggt ctacatgacg atcctcggga tcgggctcgg
     1081 cttgagcatg caggtgctgg tcctggccac gcagaactcg gtgaaggtga gcgacctcgg
     1141 tgtggccacg tcctcggtca acttcttccg atcggtcggt ggctccgtcg gcgtggccat
     1201 cttcggcgcc ctgttcaaca gccgcctgac cgccgagctc gccggcgggg cgggcgccat
     1261 ccaaggcacg atcaccccgc aggccatcga cgcccttccg gcgaccgacc aggcggtgtt
     1321 catcgacgcc ttcgccacgg cgctcaccga cgtgttcctc atcgccgtgc ccgtgatgat
     1381 cgtggccttc gtgctgacgt ggttcctgcg ggagatcccg ctcatggcca gctccggcca
     1441 ggcccgggcc ctggcccagg agggcgccgt ccagaagacc gaggtcgccg ccgtggctct
     1501 ggccggctag cggtcgatca tccgtcgggc tccgtcccgc cctggtcgag gcggaacggc
     1561 gggtactcgt cccgcagcaa caggacgtac gccagcaccc ggaacaccca ccggttcagg
     1621 cccacgatca ggtcgaacag gctccgcgcg taccggctgg tgaagagcag gccgaccccg
     1681 gcgatgacca ccaggacgcc gatcagcccg ggcccgaccc gtcggccacc ccaccacgcg
     1741 ccggcaccac ccccggcgag gatcccgatg atgatcaggt gcgggatggc caacagccag
     1801 gacttcaccc agaccaggcc ccgggagagc cgctccgggt agtcgacgtc gaagccggcc
     1861 gggtcgtcgg gctgggcctc gaacgagaac ggcgggtacc ggtccgtgcc cagggccgcg
     1921 tacgagtaga agcccaccct ccaggtccac cgcatcacgc cgacggtgta cccgaacagc
     1981 gagcgggggt agcgcccggt gaagacgatg gcgaaccagg cgatgatcca aaccacgccg
     2041 acccccaccc agaggacggc gagcacgatc acgtgcggga tgatcagcac ccacttgacc
     2101 agccacagcc agcggctgag cccgtcgtcg agatggccgg tcaggcggat cggggccgac
     2161 gacgacaccg tcgaacccac cgcaggaacg gccgcggggt gcgcggcgct gtgccgggag
     2221 agcccgacgg caccgaagac cacgagaagg gcgccaccca cgaggaacac gaacccgccg
     2281 atgagcaggc cgatccccag cggcagcaac cagtcgacct tggcggccac ggccgcgtcg
     2341 acgctgaccc cggccgagcc gtcggcgttc atcacgacga cgacccagtc cccgctctcc
     2401 aggtcccaga ccagtggctc gccgtcggtg ccctgggcca cccagaagtc ctggtccgtg
     2461 ggcggatcgg cctggtcggc accgtcccgg aactggtact cgacccggaa cgggtccacc
     2521 tcgatgtcct cgatctgggc ctgcgcgacc ccgtccaggt aagcggccac gtcagcggcc
     2581 ggcccgatgc cgacgaaggc cggagactcc cccaccccct ccacgtcgat gcgcacggtc
     2641 gccaggtccc cggcgtcgaa ccagcgggtg tcgtcgggcg acaccccgag gtccaactcg
     2701 tcggagatga tcaccggtgt cgccgtgtcg aggcgctcgc tcgacgtcga gaagtagccg
     2761 tcgtcgtcgc gctgcgtggc gtgggcccac gtcagggccg cgccggccag caccaccgcc
     2821 ccggcgacca gcacgatgag ggcaccggcg acgatcatca ggatccgccc caccggtgtc
     2881 ggtggtgccg gtggcacggc gggagcagga gtgggctcgg tcatggcccc atgttcgcgg
     2941 ttgcgaacgg gccgtgcacc gccaacggcc ccggtcgcct cgcgccggta gcttggtcgg
     3001 gtgacatcgc tggcggagcg tctcggctat gacgccgacg cccggctgct catcctcaac
     3061 tgtgacgacc tcgggtcggc ccacgccgcc aacctggccg tctacgaggc cctgcgcgac
     3121 ggcgtggcca ccagcgcctc gctcatggtc ccctgcccct gggcccggga tgccgcccag
     3181 cgctacctcg gtgaggacgt cggcgtccat ctcacgctca acgccgagtg ggacgtctac
     3241 cgctggggcc cgatcaccca ggccccctct ctgctcgacg gcgacggcgg ctttccccgc
     3301 accctcgagg acctgtggga ccacggcgac atcgacgagg tccgccggga gtgccgggcg
     3361 caggtggaac gggccatcct ctggggcttc gacgtcagcc acctcgacag ccacatgggc
     3421 ggcctgcagc tgaaggccga gttcttcgac gtctacctcg agctggccgt cgacttcggc
     3481 ctccccctcc ggctctccgg ggcctcgacc gagcacctga tcggattccc gttccgggag
     3541 ctggcggcgg cggagggcgt ggtgttcccc gaccagttcg tctacgtgca gggcgtgggc
     3601 agccgggcct cgctgcagga ggcgctgttc gacctgcgcc cgggcgtcac cgaggtctac
     3661 gtccatcccg ccgtcgactc cgccgagctg cgggcctcgt tccctgacgc cgccgcccgc
     3721 atcgacgacc acgagctggt caccagcgac acgggcatcc gggcgatgat cgagcgctcc
     3781 ggcgtccagc tcatcggctt ccgcgagctg cgtgacctcc agcgggcggg ctgaaccgcc
     3841 gtcgactagc ggaggccggc gaagaggtcg gcctcgggca gctcggactc gacccggctc
     3901 cgggccagga cgtagtcgtc gtaggggtgc accgtgcggg agacgtcgcg gggcaggccg
     3961 aaccagaacg gcgaggtcgg gtcgacctgg gtctcgtggg ccagcagtga atcgaggcgg
     4021 acgtcgtacc agtcggcgat gtcgatgctg gtggtgatct ggtcgtcctg gctcgggcgc
     4081 ttgaaccagt cctccccgaa gggggactcg atccccagct ccacgaactt ctcgtgggtg
     4141 gcctggatgc gggcccggga ccagacgacg tagtagagct tctggggctc ccacggctcg
     4201 cccatctcgg ggtaggcggt ggggtcgccg gccgcatcga aggccgggag gctgatgtcg
     4261 tggacccgca ggtggtcggg gtgcgggtag ccccgctggt cgtcgccgta ggtgatgatg
     4321 acctgtggct tctcgcgccg gatgatctcg acgaaacggc cgacggcctc cgacagcggg
     4381 gcgttggcga agttgtcggg gtgggcgttg tcctcgctgt cgggcatgcc ggaatcgcgg
     4441 tagcccagca gtacgacctc gtcgtagccg atgaccttcg ccgaccgggc cagctcctcc
     4501 cgacggacct cgtcgatccg gtcacggatc tcaggccggt ccatcgccgg gttcaggatg
     4561 tcccccgcct cgccaccggt ggccgtcacc aggacggttc gcaccccctc gtcgtggtaa
     4621 cgggcgacgg tcggggcccc cttcgaggcc tcgtcgtcgg ggtgggcgtg cacggtcagc
     4681 aggcagaggt cggccatcgg ccaggaacgt accgctgagc tagcgtgacg ccaccgacgg
     4741 acggccacca cgatggggga ccatgggcca cctgaccgcc cacgaggccg tcctcaccta
     4801 cttcgacgag gcggcccggc tgctcgagct ggacgacaag ctccacaccg tcctgcgcag
     4861 cagctaccgg gaggtgcagg cccaggtccc gctgaccctg gacaacggcg acctgatcgt
     4921 ggcccggggc ta
//
LOCUS       DASTFV010000215         4583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_802626, whole genome shotgun sequence.
ACCESSION   DASTFV010000215 DASTFV010000000
VERSION     DASTFV010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802626"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFP02_04115"
     CDS             complement(<1..504)
                     /locus_tag="VFP02_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542238.1"
                     /translation="MDIHAHLVALAARQHGLLAVWQVIATGGTRDTIKHMVRSGNWER
                     LTPTVLCRTGSAPSEGRSALSAVLDAGRRSGLSHHPAAAWFDLNGFQLRPLHVTRLRD
                     ATSTPSRLSTIHEPRALPDHHLTTHRGVAITVPSRIPFDIAALGDAKGAERALDRGWA
                     RHILDHTS"
     gene            complement(602..4222)
                     /locus_tag="VFP02_04120"
     CDS             complement(602..4222)
                     /locus_tag="VFP02_04120"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HET9542239.1"
                     /translation="MADAPRNFEDLGPNEWLVDEMYEQYRADPASVSESWQEFFADYR
                     TNGSSADAAPAPVTAPPPTTEAPAPPPAEPAPAKAAPPAVDPDGVAIKGAAARIVTNM
                     EASLAVPTATSFREIPAKLLEVNRRIMNGYLGRTQGGKVSFTHLIGYAVVRAIADEVP
                     AMNASYLEVDGKPRLVRHEHLGLGLAVDVAKDDGSRTLLVPCIRDADTLDFRGFWAAY
                     EEIIKKVRNNKLSPDDFAGTTVSLTNPGTIGTERSVPRLMPGQGLIVGVGRLDYPAAY
                     EAADPRVLADLGLSKVITVSSTYDHRIIQGAESGLFLKRVHDLLLGEDDFYEDAFRSL
                     GVPYEAVQWRPDIHPLDRDQAMLDKQMQVNSVINAHRVRGHLIADLDPLAAKEPHMHA
                     ELDPATYGLTIWDLDREFLTGNLGCNTDRLPLGEILRILRDAYCRRIGIEYMHIQEPE
                     EKRWIQEHVEGVDQVLSADEHRHILERLNAAEAFEQFLGKRYVGQKRFGLDGAESAIP
                     LVDAVLEAAAEEGLDGAVLGMAHRGRLNVLTNVVGKSYDQLFAEFEGSIDPETIQGSG
                     DVKYHLGQSGKYVSRTGAELPIELAANPSHLEAVDPVVLGMVRARQDLIDQPGTFSVL
                     PLLLHGDAAFAGQGVVAETLQSSQISGYRVGGTIHLVINNQLGFTTPPESARSSEYPT
                     DIAKMVASPIVHVNGDDPEACVRAARLAYAFRQRFHKDVVIDMICYRRFGHNEGDDPS
                     YTQPLMYKKIEARRSVRKQYTESLVNRGDLTLEEAEQALNDFQSRLQDALDQTREHVQ
                     EVGEIRAKPAPPPIGVLPHIDTALDLATVQRIFAAIDTVPEGFAIHPKLAKQFDTRRA
                     AFADGTGDVDWGLAEALALGSLLEDGTTVRLAGQDTRRGTFSHRHSVLVDYESGAEYM
                     PLAHLGERQGKWFVYDSLLSEYAALGFEYGYSVVDKDALVCWEAQFGDFVNGAMIIID
                     QFLVSAEDKWDQTSGLVMLLPHAYEGQGPEHSSGRIERFLTLCAEDNMQVAYPSTAAQ
                     YFHLVRRQMRREVRKPLIIFTPKSLLRAKQARSPVADLVSGTFEEILDDPGVTDAAAV
                     QRVILASAKVAHEATARRDETGAPVAVLRMEQLYPWPAERLDELVAKYANSESVVWLQ
                     EEPRNMGAWRFAKRYLFEGYADRLFVRAVTRSESASPATGSAAIHKQEQEQLLDDAFS
                     AL"
     gene            complement(4233..>4583)
                     /locus_tag="VFP02_04125"
     CDS             complement(4233..>4583)
                     /locus_tag="VFP02_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HET9542240.1"
                     /translation="SEVVRADHPEAFPTVDAAFYDAQWVHDLDLGDAAVIDALLADAG
                     LDPVPIGQAVARGDGDRALERSLAEARDHDVTGTPAWWVDGRLLIPGVQERDTLERWV
                     GRLQDHRKDDAGDR"
BASE COUNT          633 a         1505 c         1706 g          739 t
ORIGIN      
        1 acttgtgtga tcgaggatgt gcctggccca tccccggtcg agagcgcgtt ccgcaccctt
       61 cgcatcgccc agggccgcga tgtcgaaggg gatgcgtgac ggcacggtga tggccactcc
      121 tcgatgcgtt gtgaggtggt gatccgggag ggcccgcggt tcgtggatcg tcgacagccg
      181 gctcggcgtg ctggtcgcgt cacgcaggcg cgtgacgtgt agcggcctga gctggaagcc
      241 gttgaggtcg aaccacgccg ccgccgggtg gtgggagagc ccggaccgcc gtccggcgtc
      301 cagaacggcc gagagcgcgg accggccctc tgacggcgcc gatcccgtac ggcacaggac
      361 cgtcggcgtg agccgttccc agttgccgga acgcaccatg tgcttgatcg tgtcgcgggt
      421 gccgccggtg gcgatcacct gccagacggc gaggaggccg tgctgccgcg cggcaagcgc
      481 taccaggtgg gcgtggatgt ccatggttcc ccttggactc gtccgaactg gcgcctcaac
      541 gtggcgccgg gacgcctcaa tcgggcgcca gaacgggtgg gttgtggggg gagcgggacg
      601 gttacagggc ggagaaggcg tcgtcgagaa gctgttcctg ctcctgcttg tggatggcgg
      661 cgctgccggt ggccgggctg gcggactcgg agcgggtgac ggcccgcacg aagaggcggt
      721 cggcgtagcc ctcgaagagg tagcgcttgg cgaagcgcca ggcccccatg ttccggggct
      781 cctcctgcag ccagacgacc gactcgctgt tggcgtactt ggcgaccagc tcgtccaggc
      841 gctcggcggg ccaggggtag agctgctcca tgcggagcac ggcgaccggc gcacccgttt
      901 cgtcccggcg ggcggtggcc tcgtgggcca ccttggccga ggccaggatg acccgctgca
      961 ccgcggccgc atcggtcact cccgggtcgt cgaggatctc ctcgaacgtg ccggagacca
     1021 ggtcggccac cggggaccgg gcctgtttgg cccgcagcag tgacttgggc gtgaagatga
     1081 tcagcggctt gcgcacctca cgccgcatct gccgccgcac gaggtggaag tactgcgctg
     1141 cggtcgacgg gtaggccacc tgcatgttgt cctcggcgca cagcgtcagg aagcgctcga
     1201 tgcggcccga ggagtgctcc gggccctggc cctcgtacgc gtggggcagg agcatgacca
     1261 gacccgacgt ctggtcccac ttgtcctcgg ccgacaccag gaactggtcg atgatgatca
     1321 tcgccccgtt cacgaagtcg ccgaactgcg cttcccaaca gacgagggcg tccttgtcca
     1381 ccaccgagta gccgtactcg aagccgaggg cggcgtactc ggagagcagc gagtcgtaga
     1441 cgaaccactt gccctgccgc tcacccagat gggccagcgg catgtactcg gcgccgctct
     1501 cgtagtcgac caggaccgag tggcggtgcg agaaggtgcc ccgccgggtg tcctgaccgg
     1561 ccaggcggac ggtcgtgccg tcctcgagca gtgagcccag ggccagcgcc tcggccaggc
     1621 cccagtcgac gtcaccggtg ccgtcggcga aggccgcccg gcgggtgtcg aactgcttgg
     1681 ccagcttcgg gtggatggcg aagccctcgg gcacggtgtc gatggcggcg aagatccgct
     1741 gcaccgtggc caggtcgagg gcggtgtcga tgtgggggag caccccgatc ggcggcggcg
     1801 cgggcttcgc ccggatctcg cccacctcct ggacgtgctc ccgggtctgg tccaaggcgt
     1861 cctgcaggcg cgactggaag tcgttcaggg cctgctcggc ctcctccagc gtgaggtcgc
     1921 cgcggttgac caggctctcg gtgtactgct tgcggaccga ccgccgggcc tcgatcttct
     1981 tgtacatcag cggctgcgtg taggacgggt cgtcgccctc gttgtgaccg aaccggcggt
     2041 agcagatcat gtcgatcacg acgtccttgt ggaaccgctg gcggaaggcg taggccagcc
     2101 gggcggcccg cacgcaggcc tcgggatcgt cgccgttcac gtgcacgatc ggcgaggcca
     2161 ccatcttggc gatgtcggtc gggtactccg acgaacgggc ggactcgggc ggcgtggtga
     2221 agcccaactg gttgttgatg accaggtgga tcgtgccccc gacccggtag cccgagatct
     2281 ggctgctctg cagcgtctcg gccacgaccc cctgcccggc gaaggcggcg tcgccgtgca
     2341 gcagcagggg caggaccgag aaggtccccg gttggtcgat caggtcctga cgggcccgca
     2401 ccatgccgag cacgaccggg tcgacggcct cgaggtggga cgggttggcc gccagctcga
     2461 tgggcagctc ggcgccggtg cggctcacgt acttgcccga ctggcccagg tggtacttca
     2521 cgtcgccgga gccctggatg gtctcggggt cgatcgagcc ctcgaactcg gcgaagagct
     2581 ggtcgtagga cttgcccacg acgttggtca gcacgttgag ccggccccgg tgggccatgc
     2641 ccaataccgc gccgtccagc ccctcctcgg ccgccgcctc gagcacggcg tcgaccaggg
     2701 ggatggcgga ctcggctccg tccagcccga agcgcttctg gccgacgtag cgcttgccca
     2761 ggaactgctc gaaggcctcg gccgcgttga ggcgctcgag gatgtggcgg tgctcgtcgg
     2821 cgctgaggac ctggtcgacc ccctcgacgt gctcctggat ccagcgcttc tcctccggct
     2881 cctggatgtg catgtactcg atgccgatcc ggcggcagta ggcgtcccgc aggatccgca
     2941 ggatctcgcc caggggcagc cggtcggtgt tgcagccgag gttcccggtg aggaactcac
     3001 ggtccaggtc ccagatggtg aggccgtagg tcgccgggtc cagctcggcg tgcatgtgcg
     3061 gctccttggc cgccagcggg tcgaggtcgg cgatcaggtg cccccgcacg cggtgggcgt
     3121 tgatcacgga gttgacctgc atctgcttgt cgagcatcgc ctggtcgcgg tccaggggat
     3181 ggatgtccgg gcgccactgg acggcctcgt agggcacccc cagcgagcgg aaggcgtcct
     3241 cgtagaagtc gtcctcgccc agcaacaggt cgtggacccg cttcaggaac aggccggact
     3301 cggccccctg gatgatgcgg tggtcatagg tcgagctgac ggtgatgacc ttcgacagcc
     3361 ccaggtcggc caggacccgg gggtcggcgg cctcgtaggc cgcggggtag tcgaggcggc
     3421 cgaccccgac gatcaggccc tggcccggca tcaggcgggg gaccgaccgc tcggtgccga
     3481 tcgtgccggg gttggtgagg ctgaccgtgg tcccggcgaa gtcgtccggg gacagcttgt
     3541 tgttgcggac cttcttgatg atctcctcgt aggcggccca gaagcctcgg aagtcgaggg
     3601 tgtcggcgtc gcggatgcag ggcacgagca gggtccggga cccgtcgtcc ttggccacgt
     3661 ccacggcgag gccgaggccg aggtgctcgt gacggacgag ccgaggcttg ccgtcgacct
     3721 cgaggtagct ggcgttcatc gccgggacct cgtcggcgat ggcccggacc acggcgtacc
     3781 cgatgaggtg ggtgaagctg accttgcccc cctgggtccg cccgagatac ccgttcatga
     3841 tccggcggtt gacctcgagc agcttggccg ggatctcccg gaagctggtc gccgtgggca
     3901 ccgcgaggct ggcttccatg ttggtgacga tccgggccgc cgcgcccttg atggcgacgc
     3961 cgtcgggatc gacggccggt ggcgcggcct tggccggggc gggctcggcg gggggagggg
     4021 ccggggcctc ggtcgtcggt gggggcgcgg tcacgggcgc gggtgcggcg tcggccgatg
     4081 agccgttggt ccggtagtcg gcgaagaact cctgccagct ctcgctgacc gacgcgggat
     4141 cggcccggta ctgctcgtac atctcatcga ccaaccactc gttgggcccg agatcctcga
     4201 agttgcgtgg ggcgtcagcc atcaaggcga gcctaccgat cgcctgcgtc gtccttccgg
     4261 tgatcctgga ggcgtcccac ccagcgttcg agggtgtcgc gctcctggac tcctgggatc
     4321 aggaggcggc cgtcgaccca ccacgcgggc gtgccggtga cgtcgtggtc ccgagcctcg
     4381 gcgagcgacc gttcgagggc gcggtcgccg tccccccggg ccacggcctg gccgatcggg
     4441 accgggtcga ggccggcgtc cgccaggagg gcgtcgatga ccgcggcgtc gccgagatcg
     4501 agatcgtgga cccactgggc gtcgtagaag gcggcgtcga cggtcgggaa cgcctccggg
     4561 tgatcagccc ggacgacctc act
//
LOCUS       DASTFV010000216         3108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_820679, whole genome shotgun sequence.
ACCESSION   DASTFV010000216 DASTFV010000000
VERSION     DASTFV010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820679"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..183)
                     /locus_tag="VFP02_04130"
     CDS             complement(19..183)
                     /locus_tag="VFP02_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542241.1"
                     /translation="MTSWPRPVAWRPRRAAQTMAAPVMATVWSPMPPRWKGGSSPGAV
                     SRSAMPERAQ"
     gene            complement(208..843)
                     /locus_tag="VFP02_04135"
     CDS             complement(208..843)
                     /locus_tag="VFP02_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET9542242.1"
                     /translation="MDSTTGTTDGPLDGIRVVEMGVWVAGPAAGGILADWGADVVKIE
                     PPSGDPARIFGKLLGGDMDANPPFELDNRSKRSVMLDVTTEDGRATALELIATADVFL
                     TNIRPAALERVGLDPDALLDEDERLVYALITGFGLEGPDADRAAYDIAAYWARSGIAD
                     LLTAPGDDPPFQRGGMGDHTVAMTGAAMVCRRPMAISWPHIRNTFRSKPTV"
     gene            complement(923..1201)
                     /locus_tag="VFP02_04140"
     CDS             complement(923..1201)
                     /locus_tag="VFP02_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542243.1"
                     /translation="MRKFHPFKTNRTATTGPTDLAAVGRLVAEQGPAAARRPLAEVHR
                     QAKVLGVSPAVVDLLIDPTVPDVVRARALANVSAGIDRYSTGRAEWAA"
     gene            1412..2821
                     /locus_tag="VFP02_04145"
     CDS             1412..2821
                     /locus_tag="VFP02_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET9542244.1"
                     /translation="MDLYLEPGTRRGVANALFDQIRAAVTTGRVAVGDRLPPSRELAA
                     QLGISRQTVTTVYGRLVAEGYLEGRAGGGTFVAATAGPTPTMPVASVLRPAVPLHREA
                     PPPAPTAGAFDLRIGRTDPSLFPLVEWRQCVVAALQQVPESYGDPAGLLELRRALAHW
                     IGRSRGVEAPPEHLVVTAGAQQAVDLVSRVLLRPGDHAAVEEPGYPPIRNLWAAMGVR
                     VTPVPVDHEGIVVDRIPPDVRLIYTTPSHQSPTGATMSLARRRALLTFAEEHDVAVIE
                     DDYDSEHRHSDRPLEPLYRLDRAGRVLYVCTFAKNLAASLRLGFVVVPGSLVEATTAW
                     RELADVQPPGLTQQALHRFITEGRLERHLRRTRRVYRERHEIVRTAVDQWCTEGLLRP
                     GPRNHAGLHVSALLPEGVTETSVQARARRQGVALSRYGESIALSGTPAGLLVGFGLTP
                     ADQLPDALGGLHEALAAVA"
     gene            complement(2818..>3108)
                     /locus_tag="VFP02_04150"
     CDS             complement(2818..>3108)
                     /locus_tag="VFP02_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542245.1"
                     /translation="VGPLTVADSTKVLEVDAPGLLALEVRARPFGRGRVTFTLQATSE
                     GGTLLTVDEVPIGRLAPLRPVLDPVTAHRNEKSLADLADFLDGDRALAENKS"
BASE COUNT          412 a         1183 c         1074 g          439 t
ORIGIN      
        1 ggcctacgac atcgccgcct actgggcccg ctcgggcatc gccgacctgc tcaccgcgcc
       61 gggcgacgac ccgcccttcc agcggggcgg catgggcgac cacaccgtgg ccatgaccgg
      121 cgcggccatg gtctgcgccg ccctcctcgg tcgccaggcg accggccggg gccagctggt
      181 caccacctcg ttgctccgcc agggcgccta caccgtcggc ttcgacctga acgtgttcct
      241 gatgtggggc caggagatcg ccatcggccg acggcagacc atggccgcgc cggtcatggc
      301 cacggtgtgg tcgcccatgc cgccccgctg gaagggcggg tcgtcgcccg gcgcggtgag
      361 caggtcggcg atgcccgagc gggcccagta ggcggcgatg tcgtaggccg cccgatccgc
      421 atcgggaccc tccaggccga acccggtgat cagggcgtag acgaggcgct cgtcctcgtc
      481 caggagcgcg tccgggtcca gcccgacccg ttcgagggcg gcaggacgga tgttggtgag
      541 gaacacgtcg gcggtggcga tgagctcgag ggccgtcgcc cgaccgtcct cggtggtgac
      601 gtcgagcatg acgctgcgct tcgaccggtt gtccagctcg aagggtgggt tggcgtccat
      661 gtccccaccc agcagcttcc cgaagatccg agcggggtca ccggacggcg gctcgatctt
      721 gacgacgtcc gcgccccagt cggccaggat cccgccggcc gccggcccgg cgacccacac
      781 ccccatctcc accacccgga tcccgtccag cggcccgtcg gtggtgcccg tcgtgctgtc
      841 catggcgctc ccccttgcgc ccgcctgcct accacgactc agacgccgat ctccttcgtc
      901 gatccgcctc ccgggcgcgt gctcaggcgg cccactcggc ccggccggtc gagtaccggt
      961 cgatgccggc gctgacgttg gccagggccc gggcccggac gacgtccggc accgtcgggt
     1021 cgatgagcag gtcgaccacg gcggggctga cccccagcac cttggcctgt cgatggacct
     1081 cggccagagg ccggcgggcg gcggccgggc cctgctcggc gaccaggcgg cccacggccg
     1141 ccaggtcggt ggggcctgtc gtcgcggtcc ggttcgtctt gaagggatgg aacttgcgca
     1201 tggcgctctc ctctcgagct gtgcggtcgg tatcgggtca acctacgacg atcatggtct
     1261 gtagagaaga tccaaattag acccatctca acagaccaaa tgccgaaatc accccctatt
     1321 gcattgtcca ccgtgcgtgc cggttggtct ggtcaggtaa gacatctcga ggctggccac
     1381 cgcaccagag atggggcaga ctcgacgccc gatggacctc tatctcgagc cgggcacccg
     1441 ccggggcgtc gccaacgcgt tgttcgacca gatccgggcg gccgtcacca cgggacgcgt
     1501 cgccgtgggc gaccggttgc ctcccagccg tgagctggcg gcccagctcg gcatctcccg
     1561 gcagacggtc accaccgtct acggccgcct ggtcgcggag ggctacctcg aggggcgggc
     1621 cggaggcggc acgttcgtgg ccgccaccgc cgggcccacg cccaccatgc cggtcgcctc
     1681 cgtgctccgc ccagccgttc cgctgcatcg ggaggccccg ccgcccgccc cgaccgcggg
     1741 cgcgttcgac ctgcgcatcg gtcggaccga cccgtccctc ttcccgctcg tcgagtggcg
     1801 ccagtgcgtc gtcgccgccc tccaacaggt cccggagagc tacggcgatc ccgccgggct
     1861 gctggagctg cgccgggccc tggcccactg gatcggccgc tcccggggcg tcgaggcgcc
     1921 gcccgagcac ctggtcgtca ccgccggcgc ccaacaggcg gtcgacctcg tctcccgggt
     1981 cctgctgcgc ccgggcgatc acgcggcggt cgaggagccg ggttacccac cgatcaggaa
     2041 cctctgggcc gccatgggcg tccgggtcac ccccgtgccc gtcgaccacg agggcatcgt
     2101 ggtggaccgg atcccgcccg acgtacggct gatctacacc accccgtccc accagtcccc
     2161 gaccggcgcc accatgagcc tggctcgccg gcgggcgctg ttgaccttcg ccgaggagca
     2221 cgacgtggcc gtcatcgagg acgactacga ctccgagcac cgccacagcg accggcccct
     2281 cgagccgctg taccggctgg accgggcggg ccgcgtcctc tatgtctgca cgttcgccaa
     2341 gaacctggcc gcctccctcc gcctcgggtt cgtggtcgtg cccggctcgc tggtcgaggc
     2401 cacgacggcg tggcgggagc tggccgacgt ccagccaccg ggcctcaccc agcaggccct
     2461 gcaccggttc atcaccgagg gccggctgga gcgccacctg cgccggacgc gccgcgtcta
     2521 ccgcgagcga cacgagatcg tccggacggc ggtcgaccag tggtgcaccg agggcctgct
     2581 gcgaccgggc ccgcgcaacc acgccggcct ccatgtgtcg gccctgctcc ccgaaggggt
     2641 gaccgagacg tcggtccagg cccgagcccg tcgccagggt gtggccctca gccgctacgg
     2701 cgagtccatc gccctgtccg gcacgcccgc gggactcctg gtcggcttcg gcctcacacc
     2761 ggccgaccag ctacccgacg ccctcggtgg cctccatgaa gccctcgcgg cggtggccta
     2821 ggacttgttc tcggccaagg cccgatcgcc gtcgaggaag tcggcgagat cggccaggga
     2881 cttctcgttc cggtgagccg tgacggggtc gagcaccggg cgaagggggg ccaaccggcc
     2941 gatcgggacc tcgtcgacgg tgagcagggt cccgccctcc gaggtcgcct gcagggtgaa
     3001 ggtgacccgg ccccgtccga acggacgagc ccgcacctcg agggcgagaa ggccgggggc
     3061 gtcgacctcg aggaccttcg tgctgtcggc gacggtgagg ggcccgac
//
LOCUS       DASTFV010000217         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_823464, whole genome shotgun sequence.
ACCESSION   DASTFV010000217 DASTFV010000000
VERSION     DASTFV010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823464"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VFP02_04155"
     CDS             <1..695
                     /locus_tag="VFP02_04155"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET9542246.1"
                     /translation="IVIVVLVGGGLWWYLKDDAPAAVSLDAAVDQVDDGAATASTEAG
                     SAIEGTWTIDAETGDFDFETATGTFAGFRVNEELAGIGAATAVGRTGDVTGAFTVEGT
                     TVTEASFEVDLTTITTNESRRDNRVQDALETSRFPTATFTLTEPLELGDEAADGQSVS
                     VSAVGDLTIHGETQSVDFPLEAQLVDGTVVLVGSLDLTFSDYGVEAPTSPIALSVDDN
                     GILELQFLLTAT"
     gene            complement(757..1887)
                     /locus_tag="VFP02_04160"
     CDS             complement(757..1887)
                     /locus_tag="VFP02_04160"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HET9542247.1"
                     /translation="MRTPICDELGIEFPIFAFTHCRDVVAAVSRAGGFGVLGAVGFSP
                     EQLEVELAWIDDHVGDAPYGVDIVIPGKYEGMGDMDPDHLADELRKMVPQGHLDFAKK
                     VLADRGVPELPEEEGNSMRLLGWTEATATPQIEVALAHPKVKLIANALGTPPADVIDH
                     IHEAGLKVAALCGSPYQALKHKEAGVDIVIGQGGEGGGHTGDVGSVVLWPQIAKAIAP
                     TPLLAAGGIGSGEQIAAALAMGAQGVWAGSLWLTVEEADLPPAQKQQLLDATSRDTVR
                     SRSFTGKPCRMIRTEWTDAWEADDAPDPLPMPLQYMVSGDCVARSHRYADKALSVMFN
                     PAGQVVGQLDKVEKTSAVIQRLVEEYLDAVDRLTQLTEAANA"
     gene            complement(1899..>2822)
                     /locus_tag="VFP02_04165"
     CDS             complement(1899..>2822)
                     /locus_tag="VFP02_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9542248.1"
                     /translation="RLVSRGFTPRMIAQLVDEIERRAALVVDGVCERGEVEFVEDIAA
                     QVPVQMICEMIGLEPEIWPRMFEISNQLIGSRDDPDYQVQDPGAAAMEIYALCDAVAA
                     DRRINPREDIMTALVNAEIDGERLDDLDLNLFFVTLVVAGNETTRNLINHAMLALIDH
                     PDQAQRLRDDPSLWDTAVDEMLRWGSSIHNFRRTVTTDTELRGVPLAEGDKVVMYYAS
                     ANRDEDVFEDPHRFDVGRTPNDHVTFGGGGVHYCLGASLARAEIRATMRQLVERLPDL
                     ELAGPVSRLHSDFVNGVKTMPVRFTPTAPLS"
BASE COUNT          402 a          948 c         1033 g          439 t
ORIGIN      
        1 ccatcgtcat cgtcgtcctg gtcggtggag gtctttggtg gtacctgaag gacgatgccc
       61 ctgcggccgt ctccctcgac gccgccgtcg accaggtcga cgacggcgcg gccaccgcca
      121 gcaccgaggc cgggagcgcg atcgagggga cctggaccat cgacgccgag accggcgact
      181 tcgacttcga gacggctacg ggcaccttcg ccgggttccg ggtcaacgag gagctggccg
      241 ggatcggggc tgccaccgcc gtcggtcgga ccggcgacgt caccggcgcc ttcaccgtcg
      301 aggggacgac ggtcaccgag gcttccttcg aggtggacct gaccacgatc acgacgaacg
      361 agagccgccg ggacaatcgg gtgcaggacg cgctcgagac ctcccgcttc ccaacagcca
      421 ccttcaccct gaccgagccg ctcgagctgg gcgacgaggc ggccgacggt cagtcggtga
      481 gcgtctccgc cgtcggcgat ctcacgatcc acggcgagac ccagagcgtc gacttcccgc
      541 tggaggccca gctggtcgac ggcacggtcg tgctcgtcgg ctccctggac ctgacgttct
      601 ccgactacgg cgtcgaggcc cccaccagcc cgatcgccct cagcgtggac gacaacggca
      661 tcctcgagct ccagttcctc ctgaccgcta cctaggtcgg cccgaacttg tgccggttac
      721 accacgaatt cgtggtgcga gcagcgcaag ttcggctcag gcgttggcgg cttcggtgag
      781 ctgggtgagg cggtcgacgg cgtcgaggta ctcctcgacc aggcgctgga tgacggcgct
      841 ggtcttctcg accttgtcga gctggccgac gacctggccg gcagggttga acatgaccga
      901 gagggccttg tcggcgtagc ggtggctgcg ggccacacag tcccccgaga ccatgtactg
      961 cagaggcatg ggcagcgggt cgggggcgtc gtcggcctcc caggcgtcgg tccactccgt
     1021 gcggatcatg cggcagggct tgccggtgaa gctccggctg cggaccgtgt cccgggacgt
     1081 ggcgtcgagc agctgctgct tctgggcggg aggcaggtcg gcctcctcga cggtcagcca
     1141 cagcgagccg gcccacacgc cctgggcgcc catggccagg gcggcggcga tctgctcacc
     1201 cgagccgatg ccgccggccg ccagcagcgg ggtgggggcg atggccttgg cgatctgggg
     1261 ccagaggacg accgacccga catcgccggt gtggccgccg ccctcgccgc cctggccgat
     1321 gacgatgtcg acgccggcct ccttgtgctt cagggcctgg tagggcgagc cgcagagggc
     1381 ggccaccttg aggccggcct cgtggatgtg gtcgatgacg tcggccggcg gcgtccccag
     1441 ggcgttggcg atcagcttca ccttgggatg ggccagggcc acctcgatct ggggcgtggc
     1501 cgtggcctcg gtccagccca ggaggcgcat ggagttgccc tcctcctccg gcagctcggg
     1561 cacgccccgg tcggccagga ccttcttggc gaagtcgaga tggccctgcg ggaccatctt
     1621 gcgcagctcg tcggccaggt ggtcggggtc catgtcgccc atgccctcgt acttgccggg
     1681 gatcacgatg tcgacgccgt agggggcgtc gccgacgtgg tcgtcgatcc aggccaactc
     1741 gacctcgagc tgctcggggc tgaagccgac ggcaccgagg acgccgaagc cgcccgcccg
     1801 ggagacggcg gcgaccacgt cacggcagtg ggtgaaggcg aagatcggga actcgatgcc
     1861 gagctcgtcg cagatcgggg tgcgcatggc ggtcctcctc aggacagggg ggcggtcggg
     1921 gtgaagcgga cgggcatcgt cttgacgccg ttgacgaagt cggagtggag gcggctcacc
     1981 gggccggcca gctcgagatc gggcagacgc tcgaccagct ggcgcatggt cgcccggatc
     2041 tcggccctgg ccaagctggc gccgaggcag tagtggacgc cgccgccgcc gaaggtcacg
     2101 tggtcgttcg gggtgcggcc gacgtcgaag cggtgcgggt cctcgaagac gtcctcgtcc
     2161 cggttggccg aggcgtagta catgaccacc ttgtcgccct cggccagggg cacgccccgc
     2221 agctcggtgt cggtggtgac cgtgcgccgg aagttgtgga tggagctgcc ccagcggagc
     2281 atctcgtcca cggccgtgtc ccagagggac gggtcgtccc ggagccgctg ggcctggtcc
     2341 gggtggtcga tcagggcgag catggcgtgg ttgatcaggt tccgggtcgt ctcgttcccg
     2401 gccaccacga gggtgacgaa gaagaggttg aggtcgaggt cgtcgagccg ctccccgtcg
     2461 atctcggcgt tgaccagcgc cgtcatgatg tcctcgcggg ggttgatccg gcggtcggcg
     2521 gccacggcgt cgcacagggc gtagatctcc atggccgcgg ccccgggatc ctggacctgg
     2581 tagtcggggt cgtcgcggga gccgatgagc tggttgctga tctcgaacat gcggggccag
     2641 atctcgggct cgaggccgat catctcgcag atcatctgga ccggcacctg ggccgcgatg
     2701 tcctcgacga actccacctc cccccgctcg cagacgccgt cgacgaccag ggccgcccga
     2761 cgctcgatct cgtccaccag ctgggcgatc atccgcggcg tgaagccccg ggagaccagg
     2821 cg
//
LOCUS       DASTFV010000218         4214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_828616, whole genome shotgun sequence.
ACCESSION   DASTFV010000218 DASTFV010000000
VERSION     DASTFV010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828616"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VFP02_04170"
     CDS             complement(<1..637)
                     /locus_tag="VFP02_04170"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9542249.1"
                     /translation="MPTRNWGPWVVAAVVVALLAVGGITFWPDHGDEASESQPFVNPA
                     VPIPRAPAPDVAEHRCGTVTYTPPTASEAHRADLCRPAGDPRGAVILVHGGGGYSGDR
                     STTQEWADWYRAHGFVTLSIDYTLLGDGSPEPVYPRPEQDMKAAIQWVRIQAEPLGYD
                     PQRILLHGSSAGARLAAQAFVTPGDSWFQGPDVWPDVADDAAGLIGFYGYYD"
     gene            complement(744..1337)
                     /locus_tag="VFP02_04175"
     CDS             complement(744..1337)
                     /locus_tag="VFP02_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542250.1"
                     /translation="MRRTAVVAVLVVAGLATACVGGERPELTDETVPGRRCSADGMVV
                     GPVDSDGLPSPVAARRQELIDAAVACDYRELGRLARQDGVDIRFEGEVVPVSQWRERE
                     RDGIAILRPLAGLLGLASTRQDTEDGELITWPTAVDWPASEAADAGERADLLEVVGEA
                     GIFGWAESGGYAGWRTSIAPDGTWARFWYGPVPGEHD"
     gene            complement(1418..1993)
                     /locus_tag="VFP02_04180"
     CDS             complement(1418..1993)
                     /locus_tag="VFP02_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006952 - defense response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neocarzinostatin apoprotein domain-containing
                     protein"
                     /protein_id="HET9542251.1"
                     /translation="MRKQWGKGMLAVAVVLMVAAGCSSDDSGGDDAASDTTAAAEETT
                     TSVADTTTTTAAVTAPGEAECGETVPDCTGSVTPAEGLADGDEIAIEATGFEPNLDLG
                     ITQCASEGDPDNNMETTGAEHCNLRAVGSVTSDADGAVSTTYVVAAGQTMTANTEAGV
                     TCDAEHDCVVSVGELVPDPDAQRVTFQLKFA"
     gene            complement(1998..3032)
                     /locus_tag="VFP02_04185"
     CDS             complement(1998..3032)
                     /locus_tag="VFP02_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET9542252.1"
                     /translation="MTTETLLSVRGLEKHFPVRSGVLRRVIGHVRAVDGVDLEIQAGQ
                     TLGLVGESGSGKSTTARLVTRLIEPTAGQVVFAGADLAALSGSELRTARRRIQMVFQD
                     PYASLSPRLTVAQIVSEPLQVHKLEGGDLTTRVVELLDQVGLDASVVDRYPHEFSGGQ
                     RQRIAVARALALRPDLVVCDEPVSALDVSVQSQVINLLADLQAEQGLAYLFISHDLSV
                     VRHVSDRIAVMYLGRVVEEGPAAAVHDAPTHPYTEALVSAVPVPNPTRQRARRRIVLQ
                     GDVPSPLHPPAGCHFHPRCPHVLDICRTVDPPAFTLPDGGRVHCHLHTEGPRLAGAPV
                     RALEAINNGR"
     gene            complement(3029..>4214)
                     /locus_tag="VFP02_04190"
     CDS             complement(3029..>4214)
                     /locus_tag="VFP02_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET9542253.1"
                     /translation="PTATWGRMLQTAFQYVLVVPWQIFVPGAAITLTVLAFNLIGDGL
                     RDSLGRAGPVPRAPESGAEPDGGTDGADGLIEVRNLGIEFARGDGWVRVVEGVDLSLP
                     AGRTLALVGESGCGKTVTALALMGLLPRRTGRIATGSIRLDGRELVDLPPGAMRAIRG
                     REMAMIFQEPDTSLNPAFTVGDQIAEMLRVHQGLGRRAAMLAAVEALERVDIPDPGAR
                     ARSYPHELSGGMRQRAMIAMALACEPRVLLADEPTTALDVTIQAQILDLLASLRDELG
                     MAMVFVTHDLGVVAEIADDVAVMYAGQIVERATVDELFAHPKHPYTEALIRALPQSAT
                     PGERLATIPGQVPDPAAWPRGCHFADRCAHAQDRCRAAPVELADGVRCVRAAELHLAG
                     TT"
BASE COUNT          560 a         1497 c         1589 g          568 t
ORIGIN      
        1 cgtcgtagta gccgtagaag ccgatgagcc cggcggcgtc gtcggcgacg tccggccaga
       61 cgtccggtcc ctggaaccac gagtcaccgg gggtgacgaa ggcctgagcg gcgaggcggg
      121 cgccggcgga gctgccgtgg aggaggatgc gctgcgggtc gtagcccagc ggctcggcct
      181 ggatccgcac ccattggatg gcggccttca tgtcctgctc agggcggggg tagacgggtt
      241 cgggggagcc gtcgcccagc agcgtgtagt cgatcgacag ggtgacgaag ccgtgggccc
      301 ggtaccagtc ggcccactcc tgcgtggtcg agcggtcccc gctgtagccc ccgccgccgt
      361 gaacgaggat gaccgcgccg cgggggtccc cggctggtcg gcagaggtcc gcccggtggg
      421 cctccgacgc cgtcggcggc gtgtaggtca ccgtcccgca gcggtgctcg gccacatccg
      481 gggccggcgc acgggggatg ggcacggcgg ggttcacgaa cggctgggac tcggacgcct
      541 cgtcgccgtg gtcgggccag aaggtgatgc cgccgacggc caggagggcc accacgacgg
      601 ccgcgaccac ccaggggccc cagttccgcg tcggcatcgc ccttcccatc ggccgccgcc
      661 cgtgtgctct gagccgccgt gcacgggttc tggaacctgt cgtttgtggc gggccgcgag
      721 tttcaggttc cagaacggat gggtcagtcg tgctcgccgg ggacggggcc gtaccagaac
      781 cgggcccagg tgccgtcggg ggcgatggac gtgcgccagc cggcgtagcc gccggactcg
      841 gcccagccga agatgccggc ctcgccgacc acctcgagca ggtcagcccg ttccccggca
      901 tcggcggctt cggaggcggg ccagtcgacg gcggtcggcc aggtgatgag ctccccgtcc
      961 tcggtgtcct ggcgggtgga ggccaggccc aggaggccgg ccagcgggcg gaggatggcg
     1021 atgccgtccc gctcgcgctc gcgccactga ctcacgggga cgacctcgcc ctcgaaccgg
     1081 atgtccacgc cgtcctggcg ggccaggcga cccaactccc ggtagtcaca ggccacggcg
     1141 gcgtcgatca gctcctggcg ccgggcggcg accggcgacg ggaggccgtc gctgtccacg
     1201 gggccgacga ccatgccgtc ggccgagcaa cgtcgcccgg gcacggtctc gtcggtcagc
     1261 tcgggccgct ccccgccgac gcacgcggtg gccagtccgg cgaccaccag caccgcgacg
     1321 accgccgtcc gcctcacccc gcgcagggta accccaccgt tctggcgccc gattgcggcg
     1381 tcaggacgct gcaatcaggc gccagaacgg ggtctcgcta cgcgaacttc agctggaagg
     1441 tgacccgctg ggcgtcgggg tcgggcacca gttcgccgac cgacaccacg cagtcgtgct
     1501 cggcgtcaca ggtgacgccg gcctccgtgt tggcggtcat ggtctgcccg gcggcgacga
     1561 cgtaggtcgt cgacacggcg ccgtcggcgt ccgaggtgac cgagccgacg gcccgcaggt
     1621 tgcagtgctc ggccccggtc gtctccatgt tgttgtcggg atcgccctca ctggcgcact
     1681 gggtgatgcc gaggtcgagg ttcggttcga agcccgtggc ctcgatggcg atctcgtcgc
     1741 cgtcggccag gccctccgcg ggggtcaccg agcccgtgca gtcgggcacg gtctccccgc
     1801 actcggcctc gccgggcgcg gtcacggccg cggtcgtggt cgtggtgtcc gcgacggacg
     1861 tcgtggtctc ctcggccgcg gcggtcgtgt ccgaggcggc atcgtccccg ccgctgtcgt
     1921 cgctgctgca gccggcggcc accatcagga cgacggccac cgccagcatc cctttccccc
     1981 attgcttcct catcgcgtta cctcccgttg ttgatggctt cgagagctcg gaccggggcg
     2041 cccgccaggc gagggccctc ggtgtgcaga tggcagtgga cccggccgcc gtcgggcagg
     2101 gtgaaggccg gcgggtccac ggtccggcag atgtccagga catgagggca ccgggggtgg
     2161 aagtggcagc ccgccggcgg gtgcaacggg ctgggcacgt cgccctgcag cacgatgcgt
     2221 cggcgggccc gctggcgggt cgggttcggc accggcacgg cggacacgag ggcttcggtg
     2281 tacgggtggg tgggggcgtc gtggacggcg gcggcggggc cttcctcgac cacgcggccc
     2341 aggtacatca ccgcgatgcg gtcgctgacg tgacggacca ccgacaggtc gtgggagatg
     2401 aagaggtagg ccaggccctg ctcggcctgc aggtcggcca acaggttgat gacctgggac
     2461 tgcaccgaca cgtccagggc gctgacgggc tcgtcgcaga cgaccaggtc cgggcgcagg
     2521 gccaaggccc gggctacggc gatgcgttga cgctgcccgc ccgagaactc atgcggatag
     2581 cggtcgacga cgctggcgtc gagaccgacc tggtcgagga gctcgacgac ccgggtggtc
     2641 aggtccccgc cctcgagctt gtggacctga agcggctcgg acacgatctg ggccacggtg
     2701 agccgggggc tgagcgaggc atacgggtcc tggaacacca tctggatccg ccgccgcgcc
     2761 gtccgcagct cggacccgga cagcgcggcc aggtcggcgc cggcgaagac cacctgtccg
     2821 gcggtcggtt cgatgagacg ggtgacgagg cgggccgtcg tcgacttccc ggaaccgctc
     2881 tcgcccacca ggcccagcgt ctgcccggcc tggatctcga gatccacgcc gtccacggcc
     2941 cgcacgtgcc cgatgacgcg ccgcagcacg ccgctgcgca cggggaagtg cttctccagg
     3001 ccccgcaccg agaggagcgt ctcggtggtc acgtggtgcc cgccaggtgg agctcggccg
     3061 cccgaacgca gcggacgccg tcggccagtt cgaccggcgc ggcccggcac cggtcctggg
     3121 cgtgggcgca gcggtcggcg aagtggcagc cccggggcca ggccgccggg tcggggacct
     3181 ggccggggat ggtggccagg cgctcgcccg gcgtcgccga ctgggggagg gctcggatca
     3241 gcgcctcggt gtaggggtgc ttcgggtggg cgaacagctc gtcgaccgtc gcccgctcca
     3301 cgatctggcc ggcgtacatg acggccacgt cgtcggcgat ctcggccacc acgccgaggt
     3361 cgtgggtcac gaacaccatg gccatcccca gctcgtcccg caacgaggcc aggaggtcga
     3421 ggatctgggc ctggatggtc acgtccaggg ccgtggtcgg ctcgtcggcc aacagcaccc
     3481 ggggctcaca cgccagcgcc atcgcgatca tggcccgctg gcgcatcccg ccggacagct
     3541 cgtgggggta gctgcgggcc cgggcgcccg ggtcggggat gtcgacccgc tcgagggcct
     3601 cgaccgcggc cagcatcgcc gcccggcgcc ccaggccctg gtggacccgg agcatctcgg
     3661 cgatctggtc gcccacggtg aaggctggat tcaggctggt gtcgggctcc tggaagatca
     3721 tcgccatctc ccggccccgg atggcccgca tcgccccggg cggcaggtcg accagctccc
     3781 ggccgtccaa gcggatggaa ccggtggcga tgcgccccgt ccgccggggc aggaggccca
     3841 tgagggccag ggcggtgacg gtcttgccgc acccgctctc gccgacgagg gccagggtgc
     3901 gccccgccgg gagggaaagg tccacgcctt cgaccacccg gacccaaccg tcgccccggg
     3961 cgaactcgat gcccaggttc cgcacctcga tcaagccgtc ggccccgtcg gtgccaccgt
     4021 cgggctccgc tcccgactcc ggcgcccggg gtaccggacc ggctcggccc agggagtccc
     4081 gcaggccgtc accgatcagg ttgaaggcca acaccgtgag ggtgatcgcc gcgcccggga
     4141 cgaagatctg ccacgggacc acgaggacgt actggaacgc ggtctggagc atccggcccc
     4201 aggtcgcggt gggc
//
LOCUS       DASTFV010000219         2465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_830592, whole genome shotgun sequence.
ACCESSION   DASTFV010000219 DASTFV010000000
VERSION     DASTFV010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830592"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VFP02_04195"
     CDS             complement(<1..878)
                     /locus_tag="VFP02_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542254.1"
                     /translation="MQDVVAELRVGVKDLAVEDRAGWVPLALSDRVRDLVGLTEAMQV
                     ELVRGLASWDRATAWAEDGAVTAVAWLKANTGLTGPEASGLVRLARLYSQHRCVAAAL
                     DGGDLTLAKARLLARAEKNREGLFAECVDGFVDLARVLGNLEFSQVIEQWIDLVDDRP
                     PPDDSKRRWTSADTIGGSAHTDMFGSADDAALMRAAIEALDTPDPVDCPEGPRSRQQR
                     HYDIVIDLFRRALADQLGNDVASPTSADVILDATTAAELVADPDQGRITLEHPLLDLL
                     APFDEHTAGDRFLRRC"
     gene            complement(1252..1503)
                     /gene="rpsT"
                     /locus_tag="VFP02_04200"
     CDS             complement(1252..1503)
                     /gene="rpsT"
                     /locus_tag="VFP02_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET9542255.1"
                     /translation="MANIKSQIKRNKQNEKRRVRNKQVRSELRTRVKAAEAGGENPDE
                     TLQVAMKTIDKAAAKGVIHKNEASRRKSRLQKRADAAKA"
     gene            1561..>2465
                     /locus_tag="VFP02_04205"
     CDS             1561..>2465
                     /locus_tag="VFP02_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET9542256.1"
                     /translation="MTEISRIRNLCLLAHIDHGKSTLADRMLEICGAVELRDMRAQYL
                     DTMDLERERGITIKLQSVRLEYGEHTLHLIDTPGHVDFGYEVSRSLAACEGVVLLVDA
                     AQGIEAQTLANCYLALEHDLEIVACLNKIDLPAAEPELRAEEIEHVLGIPAEDILPIS
                     AKTGEGVTDLLDAIVERIPSPVGSEDGPLQGLIFDSHYDQFRGVISSIRVMNGRLNTG
                     DELKFMHAGVTHDADELGVRLPAPTTVPSLGPGEVGFLIAGIKDVAEARSGETVTNAR
                     RGAAEPLEGYQEPKPMVFCGLYPVEG"
BASE COUNT          405 a          842 c          838 g          380 t
ORIGIN      
        1 cagcagcggc ggaggaaccg gtccccggcg gtgtgctcgt cgaacggggc caggaggtcc
       61 aagagggggt gttcgagggt gatccggccc tgatcggggt cggcgaccaa ctccgcggcg
      121 gtggtggcgt cgaggatgac gtcggcgctg gtgggggagg cgacgtcgtt gccgagttgg
      181 tcggccaggg cccgccggaa caggtcgatg acgatgtcgt agtgccgctg ctgacgcgaa
      241 cgaggccctt cggggcagtc gacggggtcg ggggtgtcca atgcttcgat ggcggcccgc
      301 atcaacgcgg cgtcatcagc cgagccgaac atgtcggtgt gcgcggaccc gccgatggtg
      361 tcggcggagg tccagcgccg cttggagtca tcgggcggcg gtcggtcgtc gacgaggtcg
      421 atccactgct cgatcacctg actgaactcg aggttgccca gaactcgggc gagatcgacg
      481 aacccgtcga cgcactcggc gaacaggcct tcccggttct tctccgcccg ggccaacaac
      541 cgcgccttgg ccagggtcag gtccccgcca tcgagcgcgg cagccacgca ccggtgctgg
      601 gaatagagcc gggccaggcg gaccagcccg gaagcctcag gcccggtgag gccagtgttg
      661 gccttcaacc aggccaccgc agtgaccgcc ccgtcctcgg cccaggccgt ggcccggtcc
      721 caggaagcca gaccccggac caactcgacc tgcatggctt cggtgagccc gacgaggtcc
      781 cggacccggt ccgacaacgc caacggcacc cagccggccc ggtcctccac cgcgaggtcc
      841 ttgaccccca cccgcagctc ggcgaccacg tcctgcatgc ttctattctc ccaccggggt
      901 gtgacaagaa ccccccaaat cagacccccg cgacaaagaa attccgagaa gccccatacg
      961 catcgtgatg agcctgaggg ttgggctgcg ggtcgccgag ggcttcgtca ctccatgtga
     1021 cctctagccg ttctggcccc ctatttgggc gctaaagcaa ccgaatcagg ggccagaacg
     1081 gtcagaatgg ctcagagggc aacaggcggt cacgcggagt gacaattggc agcgccggtt
     1141 gcaggccaag cggtcgtccc gagccaccca gcacccccca cgggccatcg gtgcgagcgc
     1201 gacagcagag agacggaaag tcactcagac cgagtcccga aggaccggga gctacgcctt
     1261 ggcggcgtcg gcccgcttct ggaggcggga cttgcggcgg gaggcctcgt tcttgtggat
     1321 gacgcccttg gcggccgcct tgtcgatggt cttcattgcc acctgcaggg tctcatcggg
     1381 gttctcaccg ccggcctcgg ccgccttcac ccgggtgcgg agctcggaac gaacctgctt
     1441 gttgcggacg cgccgcttct cgttctgctt gttgcgcttg atctgactct tgatgttggc
     1501 catgacgggc atggacgata ccgggacggg tggctgcggc cctaccctcg ctgggttccg
     1561 atgaccgaga tctcccgaat ccgcaacctg tgcctcctgg cccacatcga ccacggcaag
     1621 tcgacgttgg ccgaccggat gctggagatc tgcggggccg tcgagctgcg ggacatgcgg
     1681 gcccagtacc tcgacaccat ggacctggag cgcgagcggg gcatcaccat caagctccag
     1741 agcgtccgcc tggagtacgg cgagcacacc ctccatctca tcgacacgcc gggccatgtg
     1801 gacttcggct acgaggtctc ccgatcgctg gctgcgtgcg agggggtggt cctcctggtc
     1861 gacgccgccc agggcatcga ggcccagacc ctggccaact gctatctggc cctggaacac
     1921 gacctcgaga tcgtggcctg tctcaacaag atcgacctcc cggcggccga gcccgagctg
     1981 cgggcggagg agatcgagca cgtgctcggc atcccggccg aggacatcct gcccatctcg
     2041 gccaagacgg gagagggggt gaccgacctg ctcgacgcca tcgtcgagcg catcccgtcg
     2101 cccgtcggca gtgaggacgg ccccctccag ggtctgatct tcgactccca ctacgaccag
     2161 ttccggggcg tgatcagctc gatccgggtc atgaacggcc gcctgaacac cggcgacgag
     2221 ctgaagttca tgcacgccgg cgtgacccac gacgccgacg agctgggggt gcgcctcccg
     2281 gcgcccacga ccgtgcccag cctcgggccg ggcgaggtcg gcttcctgat cgccgggatc
     2341 aaggatgtgg ccgaggcccg gtccggtgag acggtgacca acgcccggcg cggcgcggcc
     2401 gagcccctcg agggctacca ggagcccaag cccatggtgt tctgcggcct ctatcccgtg
     2461 gaggg
//
LOCUS       DASTFV010000220         4356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_836682, whole genome shotgun sequence.
ACCESSION   DASTFV010000220 DASTFV010000000
VERSION     DASTFV010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836682"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VFP02_04210"
     CDS             <1..611
                     /locus_tag="VFP02_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET9542257.1"
                     /translation="RAVRKRLDFDRPVEREVVEECLDIAIQAPTASNGQGYHWVVVTD
                     PETKKPIAEHYRNNFETFYGDGGGPSPYPEDDPRTDRLDAVVDSAAYLARRLHEVPVW
                     VIPCLEGRMGEGLPSFLQASLWGSIMPAVWSFMLAARERGVGTAWTTLHLPQEAEAAE
                     LLGIPYDSMTQVGLIPVAYTQGTDFKTASRVSAADRTHWDRW"
     gene            608..1006
                     /locus_tag="VFP02_04215"
     CDS             608..1006
                     /locus_tag="VFP02_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542258.1"
                     /translation="MSPGPENGHRDEVVVPDEVAGPGEPVRPGRRDRKPLIACVAGLG
                     AGWFAVFGPILVVILAPAAIVVGRRVLRRIELSGGTNAERKQAKIGFWAGIVAVILLV
                     VQLVIFQLFFEWEKDAPAIGDKAASSEPAG"
     gene            complement(1003..1776)
                     /locus_tag="VFP02_04220"
     CDS             complement(1003..1776)
                     /locus_tag="VFP02_04220"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET9542259.1"
                     /translation="MRSGANNTSIRQVRHHRYQEIADSLRRRVEAGEFVAGGLLPSEA
                     DLVARYKASRVTVRRALEDLRAEGLVDSRQGSGWFVATDPLPQNLGRLGTIEDQLLAS
                     GRTSERRILDFAFGPAPAEVAELLGTAKVLMTRRLNLADGEPFALVTVWCPEAMASKL
                     SRDDVEGRPFYELLDVELGSARQRIRAGAATAEEAELLGMLPGGPVLRCRRLTESVDG
                     RPVLASEHTFPAHRTEFVVDLSTAERSIAPSGLRLVDEG"
     gene            1812..4088
                     /locus_tag="VFP02_04225"
     CDS             1812..4088
                     /locus_tag="VFP02_04225"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HET9542260.1"
                     /translation="MAETNRTPIELIRAVYDRLPQRVTEGRERLGRPLTFAEKVLINH
                     LDDPAAEIERGKSYADLRPDRVAMQDATAQMALLQFMTAGLDEAEVPATVHCDHLIQA
                     RVDAKTDLLAAVEGNAEVYDFLESVSARYGIGFWGPGSGIIHQVVLEQYAFPGGMMIG
                     TDSHTPNAGGLGMVAIGVGGADAVDVLTGFPFNVRWPRLIGVHLTGELNGWTASKDVI
                     LKVAEILTVKGGTGAIVEYFGSGAESISATGKGTICNMGAEIGATTSLFAYDAATARY
                     LKSTGREDHVDAADAVAADLRADDEVLADPGAYFDQVIEIDLDELRPLINGPHTPDRA
                     RAVDALGAEADAEGWPKEISYALVGSCTNSSYEDITRAASIARHAADNGLRTKTDLMI
                     TPGSEQVRATIERDGLLADFDRIGATVLANACGPCIGQWARSDVSEGDANTIVTSYNR
                     NFPKRNDGLASTLAFVTSPETVVALALAGTVDFDPVTDTLTNDAGEEVTLIVPVGEEL
                     PAQGFDPGQNAFTQAPEDGSGIEVVVRPDSDRLQLLHPFDPWDGQDFVELPILVKAQG
                     KCTTDHISAAGPWLKYRGHLENISGNLFLGAVNAFTGVAGEGKDQLDGQTKPYPEIAK
                     HFHEAGQGWVAIGDENYGEGSSREHAAMEPRFRNAKAVFARSFARIAETNLKKQGVLP
                     LTFADAATYDEIGEDDRISVLGLADLEPESTVACRITKPDGTRVDFEAVQTLSPEQID
                     WFRAGSALNLIRQRVAGA"
     gene            complement(4094..>4356)
                     /locus_tag="VFP02_04230"
     CDS             complement(4094..>4356)
                     /locus_tag="VFP02_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HET9542261.1"
                     /translation="PRNARVPNVLNITDPPEITVRVGPEVELTYEDDDADTKSIMAAI
                     VDLLPAEARTYREPTGEELARTYPSGWTGDPDTESERRPGSD"
BASE COUNT          670 a         1614 c         1459 g          613 t
ORIGIN      
        1 cccgcgccgt gcgcaagcgc ttggacttcg accggcccgt ggagcgggag gtcgtcgagg
       61 aatgcctcga catcgccatc caggccccga ccgcctcgaa cggccagggc taccactggg
      121 tggtggtcac cgaccccgag accaagaaac cgatcgccga gcactatcgg aacaacttcg
      181 agacgttcta cggcgacggc ggtggcccgt ccccctatcc cgaggacgat ccccgcaccg
      241 accggttgga tgccgtggtc gactccgccg cctacctggc ccgccgcctg cacgaggtgc
      301 cggtgtgggt catcccctgc ctcgagggcc ggatgggcga gggcctgccg tcgttcctcc
      361 aggcctcgct ctggggctcg atcatgcccg cggtgtggag cttcatgctg gccgcccggg
      421 agcgcggcgt gggtaccgcc tggaccaccc tgcacctgcc ccaggaggcg gaggcggccg
      481 agctcctcgg catcccgtac gactcgatga cccaggtcgg cctcatcccc gtcgcctaca
      541 cgcagggcac cgatttcaag acggccagcc gggtgtcggc cgccgaccga acccactggg
      601 accgctggtg agcccagggc cggagaacgg gcatcgcgac gaggtcgtcg tcccggacga
      661 ggtggccggg cccggcgagc cggtccgtcc gggtcgccgt gaccgcaagc ccctcatcgc
      721 ctgtgtcgcc ggcctcggcg ccggctggtt cgccgtcttc gggccgatcc tggtcgtcat
      781 cctggccccg gccgccatcg tcgtcgggcg acgggtcctc cgtcggatcg agttgtccgg
      841 gggcaccaac gccgagcgca agcaggccaa gatcgggttc tgggccggca tcgtcgccgt
      901 gatcctgctc gtggtgcagc tggtgatctt ccagctcttc ttcgagtggg agaaggacgc
      961 gcccgccatc ggcgacaagg ccgccagcag cgagcccgcc ggctagccct cgtcgaccag
     1021 gcgcaggccg ctgggcgcga tcgagcgctc ggcggtggag aggtcgacga cgaactcggt
     1081 gcggtgggcg ggaaaggtgt gctcgctggc caggaccggg cgcccgtcga cggactcggt
     1141 cagtcgacga cagcgcagga cagggccgcc cggcagcatc ccgaggagct cggcctcctc
     1201 ggcggtggcc gctccggccc ggatgcgctg gcgggccgat cccagctcga cgtccaggag
     1261 ctcgtagaac ggccgaccct cgacgtcgtc ccgcgacagc ttcgacgcca tggcctcggg
     1321 gcaccagacg gtgacgaggg cgaatggctc accgtcggcc aggttgaggc gacgggtcat
     1381 gaggaccttc gcggtgccca acaactcggc cacctcggcc ggcgccgggc cgaaggcgaa
     1441 gtccaggatc cggcgttcgc tggtgcgccc cgaggccagg agctggtcct cgatggtgcc
     1501 cagccgcccc aggttctggg gaagggggtc ggtggccacg aaccagcccg acccctgacg
     1561 ggagtcgacc aggccctcgg cccgcaggtc ttccagcgct cgtcggaccg tgacccgact
     1621 ggccttgtac cgtgcgacca ggtccgcctc actgggcagc agaccgccgg cgacgaactc
     1681 ccccgcctcc acccggcggc gcagggagtc agcgatctcc tggtagcggt ggtggcgcac
     1741 ttgtcttata cttgtattgt tcgctccgga acgcaacccg aaccgaccag caccagagca
     1801 aggggaacga catggccgag accaaccgca caccgatcga gctgatccgc gccgtctacg
     1861 accgcctgcc ccagcgggtg accgagggcc gtgagcgcct gggtcggccc ctgaccttcg
     1921 ccgagaaggt cctcatcaac cacctcgacg accccgcggc cgagatcgag cggggcaagt
     1981 cctacgccga cctccgtcct gaccgggtgg cgatgcagga cgccaccgcc cagatggccc
     2041 tcctccagtt catgaccgcc ggcctcgacg aggccgaggt cccggccacc gtgcactgcg
     2101 accacctcat ccaggcccgg gtcgacgcca agaccgacct gttggccgcc gtcgagggca
     2161 acgccgaggt ctacgacttc ctcgagtcgg tctccgcccg gtacggcatc ggcttctggg
     2221 ggccgggttc ggggatcatc caccaggtcg tgctcgagca gtacgccttc cccggcggga
     2281 tgatgatcgg taccgacagc cacaccccca acgccggcgg cctcggcatg gtcgccatcg
     2341 gcgtgggcgg cgccgacgcc gtcgacgtgc tcaccgggtt ccccttcaac gtgcgctggc
     2401 cccggctgat cggcgtccac ctgaccggcg agctcaacgg ctggacggcg tccaaggacg
     2461 tcatcttgaa ggtggccgag atcctgaccg tcaagggcgg caccggggcc atcgtcgagt
     2521 acttcggctc cggagcggag tccatctccg ccaccggcaa gggcacgatc tgcaacatgg
     2581 gcgccgagat cggggccacc acctccctct tcgcctacga cgcggccacc gcccgctacc
     2641 tgaagtccac gggtcgcgag gaccacgtcg acgccgcgga cgccgtggcc gccgacctgc
     2701 gggccgacga cgaggtcctg gccgaccccg gtgcctactt cgaccaggtc atcgagatcg
     2761 acctggacga gctgcgcccg ctgatcaacg gtccgcacac ccccgaccgg gcccgggccg
     2821 tcgacgccct gggggccgag gccgacgccg agggctggcc gaaggagatc agctacgcgc
     2881 tggtcggctc ctgcaccaac tcctcctacg aggacatcac ccgggccgcg tcgatcgccc
     2941 gccacgccgc cgacaacggc ctgcggacca agaccgacct gatgatcacg cccggctccg
     3001 agcaggtccg agcgacgatc gagcgggacg gcctcctggc cgacttcgat cgcatcgggg
     3061 ccaccgtcct ggccaacgcc tgcgggccgt gcatcggcca gtgggcccgt tcggacgtct
     3121 ccgagggcga cgccaacacc atcgtcacct cgtacaaccg gaacttcccc aagcgcaacg
     3181 acggcctggc cagcacgctg gccttcgtga cgtcgccgga gacggtcgtc gccctcgccc
     3241 tcgccggaac ggtcgacttc gacccggtca ccgacacgct gaccaacgac gccggtgagg
     3301 aggtcacgct catcgtgccg gtcggcgagg agctgccggc gcagggtttc gaccccggcc
     3361 agaacgcctt cacccaggcc cccgaggacg gttcgggcat cgaggtcgtg gtccggcccg
     3421 actccgatcg gctccaactc ctccacccct tcgacccctg ggacggccag gacttcgtcg
     3481 aactgccgat cctggtgaag gcccagggca agtgcacgac cgaccacatc tcggccgcgg
     3541 gcccctggct gaagtaccgg ggtcacctcg agaacatcag cggcaacctc ttcctcggtg
     3601 ccgtcaacgc cttcaccggc gtggccggcg agggcaagga ccagctcgac gggcagacca
     3661 agccctatcc ggagatcgcc aagcacttcc acgaggccgg tcagggctgg gtggccatcg
     3721 gcgacgagaa ctacggcgag ggctcatccc gcgagcacgc ggccatggag ccccgcttcc
     3781 gcaacgccaa ggccgtgttc gcccgctcct tcgcccgcat cgcggagacc aacctgaaga
     3841 agcagggcgt cctgcccctg accttcgccg atgccgccac ctacgacgag atcggcgagg
     3901 acgaccgcat ctcggtgctc ggactggccg acctcgagcc ggagagcacc gtggcctgcc
     3961 gcatcaccaa gcccgacggc acccgcgtcg acttcgaggc ggtccagacc ctctcccccg
     4021 agcagatcga ctggttcagg gccggctcgg cactgaacct catccgccag cgcgtcgccg
     4081 gcgcctgacc tgatcagtcg gagccggggc ggcgctcgct ctcggtgtcg gggtcgccgg
     4141 tccagcccga cggataggtc cgggccagct cctcgccggt gggctcgcgg tacgtccggg
     4201 cttcagccgg gagcagatcg acgatggcgg ccatgatcga cttggtgtcg gcgtcgtcgt
     4261 cctcgtaggt cagctcgacc tcagggccga ccctcacggt gatctcgggc gggtcggtga
     4321 tgttcaggac gttggggacc ctggcgttcc ggggcc
//
LOCUS       DASTFV010000221         4055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_847313, whole genome shotgun sequence.
ACCESSION   DASTFV010000221 DASTFV010000000
VERSION     DASTFV010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847313"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VFP02_04235"
     CDS             complement(<1..1302)
                     /locus_tag="VFP02_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET9542262.1"
                     /translation="MRDVVEEAGRRRTFAIISHPDAGKTTLTEKFLLYGGALSGAAGS
                     VKGRGNQRAATSDWMELEQKRGISITSTVLQFPHRDHVLNLLDTPGHRDFSEDTYRVL
                     AAADAAIMVLDAAKGIEPQTLKLFEVCRDRGLPLLSFINKWDRPGRDAIELIDEIEQQ
                     IGLTPTPVTWPVGLSGDFRGVAHRGGDGGFTRFTRTSHGATMAPEEHVTAERAADEEG
                     EAWSTAQEELDLLDVIVPELDPKAFLAAEATPVFFGSALTNFGVGMLLDAVVDLVPSP
                     TPRVGEDDVPRPLDAPFAGFVFKVQANMDPSHRDRIAFFRVCSGRFERGMTVTHDKTG
                     KPFATKYAHTAFGRERETLEEAFPGDVVGLVNASELRVGDALYVDPPISFGDIPSFAP
                     NHFSSARVTDTGRYKQFRRGISQLDEEGVVQVLRDPDLGDQA"
     gene            complement(1355..2044)
                     /locus_tag="VFP02_04240"
     CDS             complement(1355..2044)
                     /locus_tag="VFP02_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542263.1"
                     /translation="MDATDGTPITIATRYEGPPGTANGGCVAGLLAASIGPSARVRLE
                     AAVPLATPLRLTARVGEARLSMGDQRLAIAHADPGPPGPIDAVSIAVAERAVAADAAD
                     HPFPGCFVCGPHSVDGLHLRPGLVDGGSTVATTWRPDPAGTGLDAEQLMWAALDCPAG
                     IAAMEPGVSAVLGTITGRIVRPAVAGETLVVVGRRLAADGRKLLAASALFGADGQAVG
                     WSESVWIVVER"
     gene            complement(2105..3907)
                     /locus_tag="VFP02_04245"
     CDS             complement(2105..3907)
                     /locus_tag="VFP02_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9542264.1"
                     /translation="MPLFSNHRPRGRRPHVVGEEPSSGPVVGDAEREVAHLSAIMAAS
                     PDLVTTVDRNGHLLYANPAANAYFGIDDEESPDWGPPPNWAVERYQQEVQPALRVTGT
                     WSGELALGRDDGEVPLACVFVAHRGDDGRIARVTVISRDLTATRQFEEDLQHRATHDP
                     LTDLPNRALLMDRLSMALARTARLATGVAVICCGVDRFKLVNEGLGHPVGDDLLRQVA
                     ERLVQSVRPGDTVARFGGDEFVLLCADLTSPRDAVVLCERIQEAVRAPILAGDNELVV
                     TLSIGIAYDEQGVSLPADLVRDADAAMYRAKTQGRDRAEVFDGALRRRALDRLDTESG
                     LRRAVDEDRLVVLYQPVIDLRDGRVSGFEALMRWDRPERGLLAPADFMEVAEETGLII
                     PLGRHLFVEACRQTVAWQQGRAGDDLSVFVNFSAAQLRHDAVVRDVATVLEQTGIDPG
                     CVQVEVTEHVLLGVEDAAVSQIAALKDLGLKIVIDDFGTGYSSLSYLQRFPVDLLKVD
                     RSFVAGVTHGEGDAAIVRGVIDLAHRLGMSAVAEGVESTEQMEALRDLGCERAQGYFL
                     GHPATAPTQTALLAGQPVTARRLTRDGRPGAGAA"
BASE COUNT          585 a         1413 c         1481 g          576 t
ORIGIN      
        1 ggcctgatcg ccgaggtcgg ggtcgcgcag gacctggacc acgccttcct cgtcgagctg
       61 gctgatgccc cggcggaact gcttgtaccg accggtgtcg gtcacccggg cactggagaa
      121 gtggttcggg gcgaagctgg ggatgtcgcc gaagctgatg ggcgggtcga cgtagagggc
      181 gtctcccacc cgcagctctg acgcgttcac gagacccacg acgtcgccgg ggaacgcctc
      241 ctccagcgtc tcccgttccc ggccgaaagc cgtgtgggcg tacttcgtgg cgaagggctt
      301 gcccgtcttg tcgtgggtca cggtcatgcc gcgctcgaag cggccggagc agacccggaa
      361 gaaggcgatg cggtcccggt gcgacgggtc catgttggcc tgcaccttga agacgaaccc
      421 ggcaaagggg gcatccaggg ggcgcgggac gtcgtcctcg ccgacccgtg gggtgggcga
      481 ggggacgagg tctacgaccg cgtccaacaa catgccgacg ccgaagttgg tcagggccga
      541 accgaagaag accggcgtcg cctccgccgc caggaaggcc ttggggtcca gctccgggac
      601 gatgacgtcg aggaggtcga gctcctcctg ggccgtggac caggcctcac cctcctcgtc
      661 ggccgctcgc tcggcggtca cgtgctcctc gggcgccatg gtggcgccgt ggctggtgcg
      721 ggtgaagcgg gtgaagccac cgtctccgcc ccggtgggcc acgccccgga agtcgccgga
      781 gaggccgacc ggccaggtca ccggcgtggg ggtcagcccg atctgctgct cgatctcgtc
      841 gatcagctcg atggcgtccc ggcccggccg gtcccacttg ttgatgaagc tcagcagggg
      901 caggccccgg tcccggcaga cctcgaagag cttgagcgtc tgcggctcga tgcccttggc
      961 cgcgtccagc accatgatcg cggcgtcggc cgcggccagg acccggtagg tgtcctcgga
     1021 gaagtcccgg tgccccgggg tgtcgagcag gttgaggacg tggtcgcggt gcgggaactg
     1081 caggaccgtc gaggtgatgg agatgccccg cttctgctcc aactccatcc agtccgaagt
     1141 ggccgcccgc tggttgcccc gccccttcac cgacccggcg gcaccactca gggcgcctcc
     1201 gtacagcagg aacttctcgg tcagggtcgt cttcccggcg tcgggatggc tgatgatggc
     1261 gaaggtcctg cggcgcccgg cttcctcgac gacgtcacgc atgggtgaag gatgcccgga
     1321 tcggcggccg gggcgaccgg cacgggcccg gccctcaacg ctcgaccacg atccagacgg
     1381 actccgacca acccaccgcc tgtccgtccg cgccgaacag ggcgctggcc gcgaggagct
     1441 tgcgcccgtc ggcagccagg cgtcgaccga cgacgaccag ggtctcgccc gcgaccgcgg
     1501 gacggacgat ccggcccgtg atggtgccga ggacagccga caccccgggt tccatggcgg
     1561 cgatccccgc gggacagtcg agggccgccc acatgagctg ctcggcgtcg agtcctgttc
     1621 ccgcggggtc cggcctccat gtcgtggcca cggtcgagcc gccgtcgacc agacccggcc
     1681 ggaggtgcag cccgtcgact gaatgcggcc cgcacacgaa gcatccgggg aacgggtggt
     1741 cggcggcgtc ggcagccacg gcgcgctcgg ccacggcgat cgagacggcg tcgatcgggc
     1801 cggggggtcc ggggtcggca tgggcgatgg cgagccgctg gtcgcccatg gacaacctcg
     1861 cctcgccgac gcgagccgtc agccgcagcg gggtggccaa ggggaccgcc gcctccaggc
     1921 ggacccgagc cgacggaccg atggatgcgg ccaacagccc ggcgacacag ccaccgttcg
     1981 ccgtgccggg tgggccctcg tagcgggtcg cgatcgtgat cggtgtcccg tcggtggcgt
     2041 ccatcccctc ccagtctgac ccacccggtc gatcgggcga ccagggcctg tgtgccccgg
     2101 cgggtcaggc cgctccggcc ccgggtcggc cgtcccgggt caggcgccgg gcggtgacgg
     2161 gctggccggc caggagggcg gtctgggtgg gggcggtcgc cgggtggccg aggaagtagc
     2221 cctgggcccg ctcgcagccg agatcccgca gcgcctccat ctgctcggtg gactcgacgc
     2281 cctcggccac agcgctcatc cccagccggt gggccaggtc gatcaccccg cgcacgatgg
     2341 ccgcatcgcc ttcgccgtgg gtgacgccgg ccacgaagga ccggtccacc ttcaacagat
     2401 cgaccgggaa ccgctggagg tacgacagcg acgaatagcc ggtgccgaag tcgtcgatga
     2461 cgatcttcag gcccagatcc ttgagtgcgg cgatttgact gacggcggcg tcctcgacgc
     2521 ccagcagcac gtgctcggtg acctccacct gcacgcatcc gggatcgata cccgtctgtt
     2581 ccagcacggt ggcgacgtcc cgaaccactg cgtcgtggcg gagctgcgcg gcggagaagt
     2641 tgacgaacac gctcaggtca tcgcccgccc gtccttgctg ccaggccacg gtctggcggc
     2701 aggcctcgac gaacaggtgt cggcccagcg ggatgatgag cccggtctcc tccgccacct
     2761 ccatgaaatc agccggagcc agcaggccgc gctccgggcg gtcccaacgc atgagagcct
     2821 cgaaccccga cacccggccg tcgcgtaggt cgatgaccgg ctggtagagc acgacgaggc
     2881 ggtcctcgtc gacggcccgt cgcaacccgg actccgtgtc gaggcgatcg agggcccggc
     2941 gacgcagggc gccgtcgaac acctcggccc gatcccgccc ctgggtcttc gcccggtaca
     3001 tggcggcatc ggcgtcccgc accaggtcgg cgggcaggct cacaccctgc tcgtcatagg
     3061 cgatgccgat gctgagggtc acgaccagct cgttgtcgcc ggccaggatc ggtgcccgca
     3121 cggcttcctg gatccgctcg cacaggacca cggcatcccg gggcgaggtc agatcggcac
     3181 agaggaggac gaactcgtcg cccccgaagc gggccacggt gtccccgggg cgcacgctct
     3241 gcaccaggcg ttccgccacc tggcgcagca ggtcgtcgcc gacggggtgg ccaaggccct
     3301 cgttgaccag tttgaagcgg tcgacaccgc agcagatcac cgccacgccg gtagccaacc
     3361 gcgccgtccg ggccagggcc atgctcaggc ggtccatgag gagggcccgg ttgggcaggt
     3421 cggtcagcgg gtcgtgggtg gcccggtgct gcaggtcctc ctcgaactgc cgggtggcgg
     3481 tgaggtcgcg gctgatgacc gtgacccggg cgatacggcc gtcgtcacct cggtgggcga
     3541 cgaagacgca ggccaggggg acctcaccgt cgtcacggcc cagggccagc tcgccgctcc
     3601 acgtgccggt gacgcggagc gcgggctgga cctcctgctg gtagcgctcg acggcccagt
     3661 tcggcggcgg accccagtcc gggctctcct cgtcgtcgat gccgaagtag gcgttggctg
     3721 ccgggttggc gtagagcagg tgtccgttgc ggtcgacggt ggtgaccagg tcgggactgg
     3781 ccgccatgat cgccgagagg tgggcgacct ctcgttcggc gtcgccgacg actgggcccg
     3841 aagagggctc ctcacccacg acgtgtggtc gccgacctcg cgggcggtgg ttggagaaca
     3901 gcggcatgac tcccttcgag cctaggagtc ggcttccgcc gcggccggga tggctaaccc
     3961 ggagggggga atgtccgtcc cttgtccagg agctcgccga tggcggggcc gggttggggc
     4021 cgaccgagaa ggaagccctg ggccaggtcg cagcc
//
LOCUS       DASTFV010000222         3891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_861687, whole genome shotgun sequence.
ACCESSION   DASTFV010000222 DASTFV010000000
VERSION     DASTFV010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861687"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VFP02_04250"
     CDS             <1..188
                     /locus_tag="VFP02_04250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9542265.1"
                     /translation="HDGHGLGRELVTDALHWLHRRGARRAVVNTQEGNVTALHLYEAL
                     GFRPEPVGLDVLGALLH"
     gene            218..2185
                     /locus_tag="VFP02_04255"
     CDS             218..2185
                     /locus_tag="VFP02_04255"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HET9542266.1"
                     /translation="MALLIPGLLSGAGPAGAQEAPDSRPRLVAQTPWVAEDQPFSMAF
                     AVHGGLPVDGTIELTLYGAITDRETLIRSGTDPTLLGDVRDVLSVPTALVPRGRDGAF
                     RLRLATDGSNAGLPVADPGVYPLAVAVAAADGTTGPPMLTFVVRTAEDAATTPLLTAV
                     VLPLHAPPAFGPDGRPVVSDRARRLLEVRAGLLDKYDDVPLTVVPTPETLAAVARVDP
                     ILLASVRAALADRHVVGGPYVRLDLAAFSGVPDLADPLQDQFQAGDRTLRRVLRKDID
                     QRTWVGAGNPTTNAIDALARAGIDRALFRQESVTGATVPINEPVVVAGATGQTIDAVL
                     ADAVLRAHVNGTGDPVLMAHRALADLALLSAPPDEGGIATDDGRAGVVLELTATRPLP
                     AAFLDTLLEGLQAGGPTRAVSLAGLLDDDPLGDPGVEPGPVVDGIALSGQDLTAYGRN
                     LAITRQTVDGYASFAGQADPLTRDLRRRLLVSGAVDLTESRRGAYLRSVSHAIADRTA
                     DVAITDDETVTLTSREGDIPLTIRNDTGAPVEVRLSFDSDNKLEFPDGATQRLRLDEG
                     PNRVEVPVVARASGAFPLRITATSPDGVLTVSRARVTVRSTAVSGVGLVLSIGALLVL
                     VVWWASHWRTARRNRRLVDPGTGNPPEPAPV"
     gene            2274..3110
                     /locus_tag="VFP02_04260"
     CDS             2274..3110
                     /locus_tag="VFP02_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET9542267.1"
                     /translation="MPVRIVTDSACDLDQALVDQHGIEVVPLSIRFGDDEFVDRIELS
                     NEEFYARMAASDTLPETAAPSPGAFEQAFRKQGASGDAVVCINLSAALSATMQSAATA
                     AASLEGEIAVTVVDSKALTLGLGNIVVAAAEAAAAGASPTDIEAKVEDLSGRTRMWGM
                     LDTLDNLLKGGRIGKAQQLMGSVLSIKPILDMSTGEVHEAAKPRTHKKGLLWLRDKVL
                     EFGDDIETLWVMAGDPDDAGELVDLLAADYTGEIGQGRIGPVIGTHGGPGVLGVTFWV
                     KP"
     gene            complement(3139..>3891)
                     /locus_tag="VFP02_04265"
     CDS             complement(3139..>3891)
                     /locus_tag="VFP02_04265"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET9542268.1"
                     /translation="DFDGPGGTAAHAYFPPPNGWSAAGDVHLDDAEHWSTTGSGVDLE
                     SVALHELGHALGLTHATAAQCPLQASTTRPIMCGTLVGVDRTLAQDDINGIQSLYGVP
                     AIACAGRAVTVDLARGQLPTGGADVILGTPGADEINSGGGDDTVCGGGGNDTVDGGAG
                     NDRIVGGGGNDALYGRTGVDRLEGGVGPDRLLAGDQDDTLYGGSGGDNLDGGTGADRL
                     YAGPQSDTCNGRAGRDSSTGCENSRGIESRIQ"
BASE COUNT          494 a         1528 c         1342 g          527 t
ORIGIN      
        1 accacgacgg ccacggcctg ggtcgggaac tggtcaccga cgccctccac tggctgcacc
       61 ggcggggggc ccgccgggcc gtggtcaaca cccaggaggg caacgtcacc gcgctgcacc
      121 tgtacgaggc gctgggcttc aggcccgagc ccgtcggcct cgacgtgctc ggggccctcc
      181 tccattgagg cggcgcgtcg ggctcctggg aacggtcgtg gccctcctga tccctggcct
      241 gctcagcggg gccggccccg ctggggccca ggaggccccg gacagccggc cccgcctcgt
      301 cgcccagacc ccctgggtgg ccgaggacca gcccttctcc atggccttcg cggtccatgg
      361 tggactgccc gtggacggca cgatcgaact gaccctgtac ggcgccatca ccgatcggga
      421 gacgctcatc cggagcggca ccgacccgac gctgctgggc gacgtccgcg acgtgctgtc
      481 ggtgcccacg gccctggtcc cccgcgggcg ggatggcgcc ttccgcctcc gcctggccac
      541 ggacggctcg aacgccggcc tccccgtggc cgaccccggc gtctatccgc tggccgtggc
      601 cgtggccgcc gccgacggca cgaccggacc cccgatgctg accttcgtcg tgcgcaccgc
      661 ggaggacgcc gccaccaccc cgctgctcac cgccgtggtg ctcccgctgc atgcgccgcc
      721 cgccttcggg ccggacggcc gcccggtcgt cagcgaccgg gcccgacgcc tgttggaggt
      781 tcgggccggc ttgctcgaca agtacgacga cgtgcccctg accgtcgttc ccaccccgga
      841 gaccctcgcg gccgtggccc gggtggatcc catcctgttg gcgagcgtgc gcgccgcgct
      901 ggccgatcgc cacgtggtcg gcggtcccta cgttcgactc gacctggccg ccttctccgg
      961 cgtgcccgat ttggccgatc ccctgcagga ccagttccag gccggcgacc ggaccctgcg
     1021 tcgggtgctc cgtaaggaca tcgaccagcg cacctgggtc ggcgcgggca accccaccac
     1081 caacgccatc gatgccctgg cccgggccgg catcgaccgg gccctgttcc gccaggaatc
     1141 ggtcaccggc gccaccgtgc cgatcaacga gccggtcgtg gtcgccgggg ccaccgggca
     1201 gaccatcgac gccgtcctcg ccgatgccgt tctccgggcc cacgtcaacg ggaccggcga
     1261 ccccgtcctg atggcccacc gcgccctggc cgacctggcg ctgctctcgg ccccgccgga
     1321 cgaaggcggc atcgccaccg acgacggccg ggccggcgtg gtgctggagt tgaccgccac
     1381 ccgccccttg cccgcggcct tcctcgacac cctgctggag ggcctgcagg cggggggccc
     1441 gactcgggcc gtgagcctgg ccgggctgct cgacgacgac ccgctcggcg atcccggcgt
     1501 ggagcccggg ccggtcgtcg acggcatcgc cctgtccggc caggacctca cggcctacgg
     1561 tcgcaacctg gcgatcaccc gccagaccgt ggacgggtac gccagcttcg cgggacaggc
     1621 cgatcccctc acccgggacc tccggcggcg cctgctggtc tccggggccg tcgacctgac
     1681 tgagagccgg cggggcgcct acctccgctc ggtcagccac gccatcgcgg accggaccgc
     1741 ggacgtggcc atcaccgacg acgagacggt gacgctgaca tcgcgcgagg gcgacatccc
     1801 cctcaccatc cgcaacgaca ccggcgcgcc ggtggaggtc cggctctcct tcgacagcga
     1861 caacaagctg gagttccccg acggcgccac ccagcgcctg cgtctcgacg agggcccgaa
     1921 ccgggtcgag gtcccggtgg tggcccgggc cagcggggcg ttccctctgc gcatcaccgc
     1981 cacctccccc gacggcgtcc tcacggtcag ccgggcccgg gtcaccgtgc gctcgactgc
     2041 cgtgtccggc gtcggcctcg tcctgtccat cggtgccctg ctcgtcctcg tggtctggtg
     2101 ggccagccat tggcgcactg cccggcgcaa ccgccgtctc gtcgaccccg gcacgggtaa
     2161 cccaccggag ccggccccgg tctgatcccg agggcagcaa ctacgctccg ccccgcaccg
     2221 ccgcccgagc cggcggtgcg gcacccctca tccactgaag acccgaggat tcaatgcccg
     2281 tccgcatcgt caccgacagc gcctgtgacc tggatcaggc gctggtcgac cagcacggca
     2341 tcgaggtggt gccgttgtcc atccgtttcg gcgacgacga gttcgtcgac cggatcgagc
     2401 tcagcaacga agagttctac gcccggatgg ccgcgtcgga caccctgccc gagaccgccg
     2461 ccccctcccc cggggccttc gagcaggcct tccgcaaaca gggggcctcg ggcgacgcgg
     2521 tcgtctgcat caacctctcc gccgcgctgt cggcgaccat gcagtcggcg gccaccgcgg
     2581 ccgcgtccct cgagggcgag atcgcggtca ccgtggtgga ctccaaggcc ctcaccctcg
     2641 ggctgggcaa catcgtggtc gccgcggccg aggccgcggc cgccggggcc tcccccaccg
     2701 acatcgaggc caaggtcgaa gacctctccg gccggacccg catgtgggga atgctcgaca
     2761 ccctcgacaa cctcctcaag ggcggccgga tcggcaaggc ccagcagctc atgggcagcg
     2821 tcctgtcgat caagcccatc ctggacatga gcaccggcga ggtccacgag gcggccaagc
     2881 cccggaccca caagaagggc ctgctctggc tgcgggacaa ggtgctggag ttcggcgacg
     2941 acatcgagac cctctgggtg atggccggcg atcccgacga cgccggcgag ctggtcgacc
     3001 tcctggccgc cgactacacc ggcgagatcg gccagggccg catcggcccg gtcatcggca
     3061 cccacggcgg gcccggcgtc ctcggcgtca ccttctgggt caagccctag ccgaccaccg
     3121 ggcggacggg cccggcggtc actggatccg ggactcgatg ccgcgggagt tctcgcaccc
     3181 ggtggaggag tcccggcccg cgcggccgtt gcaggtgtcg gactgggggc cggcgtacag
     3241 cctgtcggcg ccggtcccac cgtcgaggtt gtccccgccg ctgccgccgt agagcgtgtc
     3301 gtcctggtcc ccggccagca ggcggtcggg acccaccccg ccctcgaggc ggtcgacccc
     3361 ggtgcggccg tagagggcgt cgttgccgcc gccgccgacg atccggtcgt tgccggctcc
     3421 accgtcaacg gtgtcgttgc cgccgccgcc gcacacggtg tcgtcaccgc cgccgctgtt
     3481 gatctcgtcg gcccccggcg tgccgaggat cacatcggcc ccgccggtcg ggagctggcc
     3541 ccgagccagg tcgacggtga cggcccggcc cgcacaggcg atggccggca ccccgtagag
     3601 ggactggatc ccgttgatgt cgtcctgggc cagcgtgcgg tcgaccccca cgagggtgcc
     3661 gcacatgatc ggccgggtgg tgctggcctg gagcgggcac tgggccgccg tggcgtgggt
     3721 caggccgagg gcgtgcccga gctcgtggag ggcgacgctc tccaggtcga ccccactgcc
     3781 ggtggtggac cagtgctcgg cgtcgtcgag gtggacgtca ccggccgccg accacccgtt
     3841 cgggggcggg aagtaggcgt gggccgccgt gccccccggg ccgtcgaagt c
//
LOCUS       DASTFV010000223         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_864509, whole genome shotgun sequence.
ACCESSION   DASTFV010000223 DASTFV010000000
VERSION     DASTFV010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864509"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFP02_04270"
     CDS             complement(<1..380)
                     /locus_tag="VFP02_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542269.1"
                     /translation="MASTAVKRALVRLDRAPAWTLVIAFLAFCGAVAAWMLTGHSEGA
                     LPLVILLGVYAVAAYRPAPEVLVTAAGTAIALVIVLLGDAPGFGAPQLMSTCVAYGAA
                     MLIGWIAQSRRHRIAALESEQAEAA"
     gene            complement(471..>2342)
                     /gene="lon"
                     /locus_tag="VFP02_04275"
     CDS             complement(471..>2342)
                     /gene="lon"
                     /locus_tag="VFP02_04275"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET9542270.1"
                     /translation="GLADLVGWWPELATEQKVELLEAVDVTERVQLVLDWAKEALAER
                     ELVDKISHDVRDGLDQQKREHLLRLQMDAIRKELGEDEDGESAEDYRARIDALEAAGI
                     ADDVVRALRKELGRFERMPEQNMERGWIAGWLDTVLGLPWTERTDDELDVEVARATLD
                     ADHTGLDEVKERIVELLAVRKLRAERGLDEEGTARRKPGAIIALVGPPGVGKTSLGES
                     VASALGRRFVRVALGGVRDEAEIRGHRRTYVGAQSGRIVKAITEAGTMNPVVLLDEVD
                     KLGSDFRGDPSAALLEVLDPAQNHTFRDHYLEVDLDLSDVLFIATANVLETVPGALLD
                     RLEIIRLDGYTEDEKVIIARDHLFPRQVRSNGLHTEEAEVTDAALHGIVAGYTHEAGV
                     RELERQLGKALRKVAVRVAGGAPTPVVVDDADLPDLLGRPRFHAEDLTDRVSVPGVAT
                     GLAVTGAGGDVLFVEATAMDGEVGLQVTGQLGDVMKESAQIALSWVRSHREDIPVDAD
                     ALARRFHVHVPAGAVPKDGPSAGIAMTTALVSLLTGRAVRGEVGMTGEVTLSGRVLPI
                     GGVKQKVLAAHRAGLTEDILPARNGPDLDDLPDEVRAAMTVHLVDDVAQVLDLALAA"
BASE COUNT          323 a          861 c          829 g          329 t
ORIGIN      
        1 gcggcctccg cctgctcgct ctcgagggcg gcgatccggt gccggcggga ctgggcgatc
       61 cagccgatca gcatcgccgc gccgtacgcc acacaggtgg acatcaactg gggcgccccg
      121 aaaccggggg cgtcgccgag gaggacgatg acgagcgcga tcgcggtccc ggccgccgtg
      181 accaacacct ccggggcggg gcgataggcc gccacggcgt agacacccag caggatgacg
      241 aggggaaggg cgccctcgct gtggccggtc agcatccagg ctgcgaccgc accgcagaag
      301 gcgaggaagg ctatgacgag cgtccacgcc ggcgctcggt ccaaccgcac gagcgcccgt
      361 ttcaccgcgg tcgacgccac ccgccgaccg tactcatgcg tcacggaccg acgcatccgc
      421 cggcaggccg agtctccgct acgtcgccgg acgtagacga ccggtccggt ctaggcggcc
      481 agcgccaggt ccagcacctg ggccacgtcg tccacgaggt gcaccgtcat ggcggcccgg
      541 acctcatcgg ggaggtcgtc gaggtcgggc ccgttgcggg ccgggaggat gtcctcggtg
      601 agccccgccc gatgggcggc caggaccttc tgcttgacgc cgccgatcgg cagcacccgg
      661 cccgagagcg tgacctcgcc ggtcatcccc acctcgccgc gcaccgcccg ccctgtcagc
      721 agggagacca gcgccgtcgt catggcaatc ccggcggacg gaccgtcctt gggcaccgcc
      781 ccggcgggga cgtgcacgtg gaaccggcgc gccagcgcgt cggcgtcgac cgggatgtcc
      841 tcgcggtggc tgcgcaccca cgagagggcg atctgcgccg actccttcat cacgtcgccg
      901 agctgcccgg tgacctgcag gccgacctcg ccgtccatgg cggtggcctc gacgaacaac
      961 acgtccccac cggcgcccgt gacggcgagg cccgtggcca cgccgggcac cgagacacga
     1021 tcggtcaggt cctccgcgtg gaagcgcggc cgacccagca gatcagggag atcggcatcg
     1081 tcgaccacga cgggggtggg ggcgccaccg gccacccgaa cggcgacctt gcgcagggcc
     1141 ttgcccagct ggcgctcgag ctcgcgtacc ccggcctcat gggtgtagcc ggcgacgatc
     1201 ccgtgcaggg cggcgtcggt gacttcggcc tcctcggtgt gcaacccgtt gctccggacc
     1261 tgccggggga acaggtggtc gcgggcgatg atgaccttct cgtcctccgt gtacccgtcg
     1321 agccggatga tctcgagacg gtccagcagt gccccgggca cggtctcgag cacgttcgcc
     1381 gtggcgatga acagcacgtc cgacaggtcg aggtcgactt cgaggtagtg gtcgcggaac
     1441 gtgtggttct gggcggggtc gaggacctcg aggagcgccg ccgacgggtc gccccggaag
     1501 tcgctgccca gcttgtcgac ctcgtcgagc agcaccaccg ggttcatggt gcccgcctcg
     1561 gtgatggcct tcacgatgcg gcccgactgg gcgcccacgt acgtgcggcg gtgtcctcgg
     1621 atctccgcct cgtcgcgcac gccgccgagg gcgacgcgga cgaagcgccg tccgagggcg
     1681 ctggccaccg actcgcccag cgacgtcttg ccgacgcccg gagggccgac gagggcgatg
     1741 atggcccccg gcttgcggcg ggcggtgccc tcctcgtcga ggcctcgctc ggcccgcagc
     1801 ttgcggacgg cgagcagttc cacgatccgc tccttgacct cgtcgaggcc ggtgtggtcg
     1861 gcgtcgaggg tggcccgagc cacctcgaca tccagctcgt cgtcggtccg ctccgtccac
     1921 ggcaggccga ggacggtgtc gagccagccc gcgatccagc cccgctccat gttctgctcg
     1981 ggcatgcgct cgaagcgccc cagctccttg cgcagggccc ggacgacgtc gtcggcgatc
     2041 ccggcggcct cgagggcatc gatgcgagct cggtagtcct cggcggactc gccgtcctcg
     2101 tcctcgccca gctccttgcg gatggcgtcc atctgcaggc ggagcaggtg ctcgcgcttc
     2161 tgctgatcga gcccgtcccg cacgtcgtgg ctgatcttgt cgacgagctc acgctcggcg
     2221 agggcctcct tggcccagtc gagcacgagc tggacccgct cggtcacgtc gaccgcctcg
     2281 agcagctcga ccttctgctc ggtggcgagc tcgggccacc agccgacgag gtcggcgagg
     2341 cc
//
LOCUS       DASTFV010000224         3807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_867439, whole genome shotgun sequence.
ACCESSION   DASTFV010000224 DASTFV010000000
VERSION     DASTFV010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867439"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /locus_tag="VFP02_04280"
     CDS             <1..1301
                     /locus_tag="VFP02_04280"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET9542271.1"
                     /translation="IRVWAAPDDEPELEIEAGRSANDGPFADLVDLAGGDQDDDLDDL
                     LGPGPDDDLPHWGDPATADDDDVAGVAGGPRWRGQGEESGEPEDFSDLADETRVESEG
                     EDEMIFDDLGVAAAAIESPAGPPQRRSPYPEPPEDEFADDGGGGGGGFLGGLGGGGGG
                     RDMQQAVIVGVGLVVLLFVCAAIGAKALVALVTIVVVLATAEFFTATRRAGYHPAVPV
                     GLVGAGGFVLATYWRGEGAIPLMLFLVVVTSLCWWLFDAGPADHAVANVGITVFGVMY
                     VGGFGSFAALLLKFPDGIGMLFGAILVSVAADIGGLFVGQRMGRSPLTAASPNKTIEG
                     FVGGIVVAFVAAVLFLAVVPGVFPWEFKDAAVLGIVGGVLTPLGDLCVSRLKRDLGVK
                     DMGNLLPGHGGLLDRFDGLLFVLPATYYVVRLLEVYLNVP"
     gene            1314..2468
                     /locus_tag="VFP02_04285"
     CDS             1314..2468
                     /locus_tag="VFP02_04285"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET9542272.1"
                     /translation="MTSVAVLGATGSIGTQTLDVVRAEPGRYEVVALGACRSVDALAE
                     QAKEFRPKVVAVVDRAAAAEIADRLPPGTELTVGESALADAAREADVVVNGVVGFAGL
                     GVTLAALGAGRRLALANKESLIAAGPVVQRVRSTPGAELIPVDSEHGAIHQCLRANDV
                     AERVARLVVTASGGPFRGRSRAELAGVTIDDALAHPTWSMGPKITIDSSTLMNKGLEV
                     IEAHELFGVAYDDIDVVVHPQSVVHSMVEYTDGSTIAQLSMPDMRLPIGYALAYPDRS
                     TAPFGRIDWATLGSLDFEAPDRDAFPCLGLAYEAGRAGETAPAWLNAANEIAVEAFLE
                     GRIGWLAISDVLKATLNRHDGTKATSADVVMEADRQARTVAREIVTALTP"
     gene            2483..>3807
                     /locus_tag="VFP02_04290"
     CDS             2483..>3807
                     /locus_tag="VFP02_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9542273.1"
                     /translation="MSETELIPPTPEGPIAADQEPEGDIAGVLGLVGLVGALVALGLF
                     GGWPILAVVVSLIVMIFMHELGHYITAKRAGMKVTEFFIGFGPRIWSFQRGETEYGVK
                     AIPAGAYVRIIGMNSLEEVDPADESRTYRQKSYPRRMSVAVAGSAMHFLMALVLAFVS
                     LLAFGAITDNSARNWTVDELSTPADIELQYPNAQFNESFQQLLDDGETPSTVAGLEPG
                     DRIVSAGGVEFADYTDFREYVRAHPGESVSLVIERGDETIDATLPIGSVGVGEAATGV
                     IGLAAEEPRERLGVLPAARGSVEEVGTLMTDSVSAIGHFFTPSGLSSFADAVFSPAED
                     EEPASSGSTAASSSSSDEEGRIISIVGAARLGAQATDANGLVALFQIMIVLNVFIGLF
                     NLIPLLPFDGGHVAIGTYERIREIGHPGRRYQANLTRMLPVAYAVVLFMV"
BASE COUNT          519 a         1344 c         1335 g          609 t
ORIGIN      
        1 acatccgcgt gtgggccgct cccgacgacg agcccgagct cgagatcgag gccgggcgct
       61 cggccaacga tggcccgttc gccgacctcg tcgatctcgc cggcggtgac caggacgacg
      121 acctcgacga cctgttgggc cccgggcccg acgacgatct gccccattgg ggagatccgg
      181 cgacggccga cgacgacgac gtggccggcg ttgccggtgg accgcgctgg cggggtcagg
      241 gcgaggagag cggcgagccg gaggatttct ccgacctcgc cgacgagacc cgtgtcgaat
      301 ccgaggggga ggacgagatg atcttcgacg acctgggcgt ggccgcggcc gccatcgagt
      361 cgccggcggg cccgccccag cggcgcagcc cctacccgga gccccccgaa gacgagttcg
      421 ctgacgacgg cggtggtggc gggggcggct tcttgggcgg cctcggcgga gggggtggtg
      481 gtcgcgacat gcagcaggcc gtcatcgtcg gcgtcggcct ggtcgtcctg ctcttcgtct
      541 gtgccgccat cggggccaag gcgctggtcg cgctggtcac catcgtggtg gtcctggcca
      601 ccgccgagtt cttcaccgcc acccgccgcg cgggttacca cccggcggtc cccgtcggcc
      661 tggtcggcgc cggggggttc gtgctcgcca cgtactggcg gggtgaaggg gcaatacccc
      721 tcatgctctt cctggtcgtg gtcaccagcc tctgctggtg gttgttcgac gccgggccgg
      781 ccgaccacgc cgtggccaac gtcggaatca ccgtgttcgg ggtcatgtac gtcggtggct
      841 tcgggtcctt cgccgccctc ctgttgaagt tccctgacgg catcgggatg ctgttcggcg
      901 ccatcctcgt ctcggtggcc gcggacatcg gcgggctctt cgtcggccaa cgcatggggc
      961 gttctccgct caccgcggcc agtcccaaca agaccatcga aggtttcgtc ggcggcatcg
     1021 tcgtggcgtt cgtggccgcg gtgttgttcc tcgccgtcgt gccgggcgtg ttcccgtggg
     1081 agttcaagga cgcggctgtc ctggggatcg tcggtggcgt gttgacgccg ctgggcgacc
     1141 tgtgcgtgtc ccgcctgaag cgcgatctcg gcgtgaagga catgggcaac ctgctgcccg
     1201 gtcacggcgg actgctcgac cgcttcgacg ggctgctctt cgtgctcccg gcgacctatt
     1261 acgtcgtccg gctgctcgag gtctacctga acgtgccgtg agccggtgac cccatgacct
     1321 cggtcgcggt gctgggggcg acgggctcga tcggcacgca gacgctggat gtggtgcggg
     1381 ccgagcccgg gcggtacgag gtcgtggccc tcggagcctg ccgatcggtc gacgccttgg
     1441 ccgagcaggc caaggagttc cggccgaagg tcgtggccgt cgtcgaccgg gccgcggcgg
     1501 ccgagatcgc cgaccggctc ccgcccggaa ccgagctgac cgtgggcgag tcggccctgg
     1561 ccgacgccgc ccgcgaggcc gacgtggtcg tgaacggggt cgtcggcttc gccggcctcg
     1621 gcgtgaccct ggccgcgctc ggggccggcc gtcgactggc cctggccaac aaggagtcac
     1681 tcatcgccgc gggtccggtc gtgcagcggg tccgctccac gccgggggcc gagctcatcc
     1741 cggtcgacag cgagcacggc gccatccacc agtgcctccg ggccaacgac gtggccgagc
     1801 gggtggcccg cctcgtggtc acggccagcg gagggccgtt ccggggccgt tcccgggcgg
     1861 agctggccgg cgtcaccatc gacgacgccc tggcccatcc cacctggtcc atgggcccga
     1921 agatcaccat cgactcgtcc acgctgatga acaagggcct ggaggtcatc gaggctcacg
     1981 agctgttcgg ggtcgcctac gacgacatcg acgtggtcgt gcacccgcag tcggtcgtgc
     2041 actcgatggt cgagtacacc gacggctcga ccatcgccca gctgtcgatg ccggacatgc
     2101 gcctgcccat cggatacgcc ctcgcctacc cggatcggtc gacggccccg ttcgggcgca
     2161 tcgactgggc caccctcggc tcgctggact tcgaggcccc tgaccgggac gccttcccct
     2221 gcctcggcct cgcctacgag gccgggcggg cgggcgagac cgcgccggcc tggctcaacg
     2281 cggccaacga gatcgcggtc gaagccttcc tggagggccg catagggtgg ttggccatca
     2341 gcgacgtgct caaggccacg ctcaaccggc acgatggaac gaaagcgaca tcggccgacg
     2401 ttgtgatgga ggccgaccgt caggcccgta cggtggcgcg cgagatcgtc accgccctca
     2461 ccccctagag caagcggagc gcatgtccga gaccgagctg atccctccga cacccgaagg
     2521 gccgatcgcg gccgaccagg agccggaggg tgacatcgcc ggcgtcctcg ggctcgtcgg
     2581 actggtcggc gccctcgtcg ccctcggcct gttcgggggc tggcccatcc tggccgtcgt
     2641 cgtgtcgctc atcgtcatga tcttcatgca cgagctgggc cactacatca cggccaagcg
     2701 ggccgggatg aaggtcaccg agttcttcat cggcttcggg cctcgcatct ggtcgttcca
     2761 acggggcgag accgagtacg gcgtcaaggc cattccggcc ggggcctacg tccgcatcat
     2821 cggcatgaac agcctcgagg aagtcgaccc ggccgacgag tcccggacgt accgccagaa
     2881 gtcctacccg cgacgcatgt ccgtggccgt ggccggctcg gccatgcact tcctcatggc
     2941 cctggtcctg gccttcgtga gcttgctggc cttcggtgcc atcaccgaca acagtgcgcg
     3001 gaactggacg gtcgacgagc tctccacgcc cgcggacatc gagctgcagt acccgaacgc
     3061 ccagttcaac gagtcgttcc agcagctgct cgacgacggc gaaacgccct cgaccgtcgc
     3121 cgggctcgag cccggcgatc ggatcgtgtc cgccggtggt gtcgagttcg ccgactacac
     3181 cgacttccgt gagtacgtcc gggcccaccc cggcgagtcg gtcagcctcg tgatcgagcg
     3241 gggcgacgag accatcgacg ccacgctgcc gatcggctcg gtcggcgtcg gcgaggcggc
     3301 cacgggggtc atcggcctcg ccgccgaaga gccgcgtgag cgactcggag tcctgccggc
     3361 ggcccggggc tcggtcgaag aggtgggcac gctcatgacg gactcggtct cggcgatcgg
     3421 ccacttcttc acgccgagcg gcctcagcag cttcgccgat gccgtgttca gcccggccga
     3481 ggacgaggag ccggcatcgt cggggagcac ggcggcgagc tcgtcgagca gcgacgagga
     3541 aggccggatc atctccatcg tcggcgccgc ccgcctcggt gcacaggcca ccgacgccaa
     3601 tggcctggtg gccctgttcc agatcatgat cgtgctgaac gtcttcatcg gcctcttcaa
     3661 cctgatcccg ctcctgccct tcgacggcgg ccacgtcgcc atcgggacct acgagcggat
     3721 ccgggagatc ggccaccccg gccggcgcta ccaggccaac ctgacccgga tgctgccggt
     3781 ggcctacgcc gtggtcctct tcatggt
//
LOCUS       DASTFV010000225         3502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_883340, whole genome shotgun sequence.
ACCESSION   DASTFV010000225 DASTFV010000000
VERSION     DASTFV010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883340"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VFP02_04295"
     CDS             <1..630
                     /locus_tag="VFP02_04295"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HET9542274.1"
                     /translation="LMVIVDADTGYGNPLNTIRTVELWEAAGATGMFLEDQVWPKKCG
                     HMAGKVVVPKEDWLAKLEAAVDHRTSLHITARTDARAAVSLDDAIERARMALELGVDA
                     VFIEAPESLAELEQIAAALPDCVRVANMIETGKTPLLTPAELHDMGFDLIVAPLGGLY
                     AAIKALTGAYATLAAKGTLRDDLDGMLSFDEFNALVDLDEHYQREERFR"
     regulatory      656..770
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            815..2008
                     /locus_tag="VFP02_04300"
     CDS             815..2008
                     /locus_tag="VFP02_04300"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HET9542275.1"
                     /translation="MTERSRILPVTGAWRPGDPEGNRRFLSFDPDRPFVLEGGAQLRG
                     LTVAYETWGELDPTGSNAVLVCHAVTGDSHLTGRSGHGHPTAGWWEALVGPGRPLDTD
                     RWFIVCVNVLGGCQGSTGPASEDPATGRPYGSTFPVVTIRDIVRTQAAVADHLGVDQW
                     LGVVGGSFGGMQVLEWGVMYPERMRALVPIATSMAASAWQISFSRVGRLAVQLDPKWR
                     GGDYYDAAPGDGPHAGLALARMIAHITYRSDAVYEAKFGRDALEQLEEFALEQRFEVE
                     GYLDYQGEKFVRRFDANSYLRLNKAMDLHDIGRNRGGTARALRRVTAPTLNLSISSDT
                     LYPRHQQEATHTGLLAGGALSYHVTVDSPHGHDGFLIETDPVGAVLDDFLTRVEKNDV
                     ETHDG"
     gene            2001..3197
                     /locus_tag="VFP02_04305"
     CDS             2001..3197
                     /locus_tag="VFP02_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9542276.1"
                     /translation="MADPSQRPTPHPETRAITGGRKDQHGALAPLLWPSTTFETESAE
                     DAARLAKSVDPDRFYSRHGNPGVRAFEQAVADLEGADAARAYASGMGAVTGVILGICS
                     AGDHIVAQHQLYSQTTQVLLGICPRFGIDVTLVDGTEPGAFAAAVRPGKTTLVLAETP
                     ANPRLDLVDLDEIGAIKGPVTCVDSTFATPLGQQPLAHGADLVLHSATKGIAGHNDAS
                     LGIVCGSQELIDWLWGFAIFQGANASPFDAMNGVRGLRTLGVRLRRQSETAQTLAEGL
                     EASPHVDGVRYPGLESHPQRDLAKRQLAHMGAIISFDVAGGREAGRAFVDALELARMA
                     TSLGGPETIVCHPASTTHQSLLPEELEAMGIGPGTIRMSVGLEHADDLADDLAQALET
                     AVRTTS"
     gene            complement(3161..>3502)
                     /locus_tag="VFP02_04310"
     CDS             complement(3161..>3502)
                     /locus_tag="VFP02_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542277.1"
                     /translation="EENTGLRISHPVFRRYEDVVAVVMGAGGGDLYVGERLAAVADLV
                     TRTTVAPPAAVVARLFGMNLVTWRDHPAAAGIDVGGLADDLQELEASTERGLVLFDLA
                     QLVVRTAVSSA"
BASE COUNT          543 a         1265 c         1197 g          497 t
ORIGIN      
        1 ctcatggtca tcgtcgacgc cgacaccggc tacggcaacc cgctcaacac catccggacc
       61 gtcgagctct gggaggccgc cggggccacc gggatgttcc tggaggacca ggtctggccc
      121 aagaagtgtg gccacatggc gggcaaggtc gtcgtcccca aggaggactg gctggccaag
      181 ctcgaggccg ccgtcgacca ccgcacgagc ctgcacatca ccgcccgcac cgatgcccgg
      241 gcggcggtga gcctcgacga cgccatcgag cgggcccgga tggccctgga gctgggcgtc
      301 gacgcggtgt tcatcgaggc gccggagtcg ctggccgagc tggagcagat cgcggcggcc
      361 ctgcctgact gtgttcgggt ggcgaacatg atcgagacgg gcaagacccc actgctcacc
      421 ccggcggagc tgcacgacat gggcttcgat ctcatcgtgg cgccgctcgg cggcctctac
      481 gccgcgatca aggcgctgac cggggcctac gcgacgctgg cggccaaggg caccctgcgc
      541 gacgacctcg acgggatgct ctccttcgac gagttcaacg ccctcgtcga cctcgacgag
      601 cactatcaac gcgaagaacg cttccgatag gtaccgggtc gggggtagcc tctcgcaaca
      661 acatcaagag cgatccgggc gaccgggtcc ggcaacctgg ggcggacacc caaggtgcca
      721 cccactccgg tggggatgtg gcctcgtgag aggcaacgaa gtcgtcccgt tcgacgtcag
      781 atgtccgccc cccgatggat cgaggtggtg agacatgacg gagcggtccc ggatcctgcc
      841 ggtcaccggt gcctggcgcc ccggcgaccc cgagggcaac cgccgcttcc tgtcgttcga
      901 ccccgaccga ccgttcgtgc tcgagggcgg ggcccagctc cgcggcctca ccgtggcgta
      961 cgagacctgg ggcgagctcg accccaccgg gtccaatgcg gtgctcgtct gccacgccgt
     1021 gaccggcgac agccatctca ccggccgttc tggccacggc cacccgacgg cgggctggtg
     1081 ggaggcgctg gtcggtcctg gtcggccgct ggacaccgac cggtggttca tcgtctgtgt
     1141 caacgtgctc ggcggatgtc aaggctcgac cggtcccgct tcggaggatc cggcgacggg
     1201 gcgaccgtac gggtcgacct ttcccgtcgt gaccatccgg gacatcgtcc gtacccaggc
     1261 ggcggtcgcc gaccacctcg gtgtcgacca gtggctgggt gtcgtggggg gctcgttcgg
     1321 cggcatgcag gtcctcgagt ggggggtgat gtacccggaa cgcatgcggg ccctggtccc
     1381 catcgccacc tcgatggcgg ccagcgcgtg gcagatctcg ttcagtcgtg tgggccgcct
     1441 ggcggttcaa ctcgacccca agtggcgggg tggtgactac tacgacgccg cgcccgggga
     1501 cgggccccac gccgggctcg cgttggcccg gatgatcgcc cacatcacct accgcagtga
     1561 cgcggtgtac gaggccaagt tcggacgcga cgcactcgag cagctcgagg agttcgcgtt
     1621 ggagcaacgc ttcgaggtgg agggttatct ggactaccag ggcgagaagt tcgtccgccg
     1681 cttcgacgcc aacagctacc tgcgcctgaa caaggccatg gacctgcatg acatcggccg
     1741 caaccggggc ggcaccgcgc gggccctgcg ccgggtcacg gctccgaccc tcaacctgag
     1801 catctccagc gacaccctct acccccgcca tcaacaggag gcgacccaca ccggcctgct
     1861 cgccggcggc gccctcagct atcacgtgac cgtggacagt ccccacggcc acgacggctt
     1921 cctgatcgag accgaccccg tcggcgcggt gctggacgac ttcctcaccc gagtcgagaa
     1981 gaacgatgtg gagacccacg atggctgacc cgtcccaacg tccgacccca caccccgaga
     2041 cccgggccat aaccggcggg cgcaaggacc aacacggcgc gctggccccc ctgttgtggc
     2101 cctcgaccac cttcgagacc gagtcggcgg aagacgccgc tcgactggcc aagtcggtcg
     2161 accccgatcg cttctacagc cgccatggca atcctggcgt gcgggccttc gagcaggccg
     2221 tagccgacct cgaaggggcc gacgcggccc gggcctacgc ctcggggatg ggggcggtga
     2281 ccggcgtcat cctcggcatc tgttccgcgg gggaccacat cgtcgcccag caccagctct
     2341 actcgcagac gacgcaggtg ctgctcggca tctgtccccg cttcggcatc gacgtcacgc
     2401 tggtcgacgg gaccgagccc ggggccttcg ccgcggcagt acggcccggg aagacgacac
     2461 tggtgctggc cgagacgccg gcgaaccctc gactcgacct ggtcgacctg gacgagatcg
     2521 gggccatcaa gggcccggtg acctgcgtgg actccacctt cgccaccccg ctcggccagc
     2581 agcccctggc ccacggtgcc gacctggtgc tccactcggc cacgaagggc atcgccggcc
     2641 acaacgacgc cagcctcggc atcgtctgcg gcagccagga gctcatcgac tggctgtggg
     2701 gcttcgccat cttccagggg gccaacgcct cgccgttcga cgccatgaac ggcgtgcggg
     2761 gcctgcgcac gctgggcgtc cggctacggc gtcagagcga gacggctcag acgctggccg
     2821 aagggctgga ggcctcgccc catgtcgacg gcgtgcgcta cccaggcctc gagtcccatc
     2881 cgcaacgcga cctggccaag cgccagttgg cgcacatggg ggcgatcatc agcttcgacg
     2941 tggccggtgg ccgcgaggcc ggccgcgcgt tcgtcgacgc cctcgagctg gcgcgcatgg
     3001 cgacctccct cggggggccg gagaccatcg tctgccaccc ggcgtcgacc acgcaccaga
     3061 gcctcctgcc cgaggagctg gaggcgatgg gcatcggccc gggcacgatc cggatgtcgg
     3121 tcggtctgga gcacgccgac gacctggccg atgacctggc tcaggcgctg gagacggccg
     3181 tccgcaccac gagctgagcg aggtcgaaca gcacgagccc gcgctccgtg ctcgcttcga
     3241 gctcctgcag gtcgtcggcc agcccgccga cgtcgatgcc ggccgccgcc gggtggtcgc
     3301 gccacgtcac gaggttcatg ccgaacaggc gggccaccac tgccgccggt ggggccaccg
     3361 tcgtgcgggt gaccaggtcg gccacggccg ccaggcgttc accgacgtac aggtcgccgc
     3421 ccccggcgcc catgaccacg gcgacgacgt cctcgtatcg gcggaagacg gggtgggaga
     3481 tgcggaggcc ggtgttctcc tc
//
LOCUS       DASTFV010000226         3124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_884262, whole genome shotgun sequence.
ACCESSION   DASTFV010000226 DASTFV010000000
VERSION     DASTFV010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884262"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VFP02_04315"
     CDS             <1..1023
                     /locus_tag="VFP02_04315"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HET9542278.1"
                     /translation="HLVNITLQNGSAAGDGGAVLVLNASSVHAVDARFDSNEASGSGG
                     ALEIPLEAFVARSDFTGNVAASGDGGALDAAAAAGQFRIRQSQFVENHAPAGEGGAVS
                     AIGFGSISTGDGVRGSSFVGNTARRGGALVLSSGIGVANSTIVNNMATVGGGGILAET
                     TSASAGTYLTVTGNAAPDGANLSYRGHDLHLFFSVIAEPEGGGENCHITGSIHPNLVH
                     TDDHSCGHHQVNSGPPQLGPLTDNGGRTLTREPLAGSPLLDAIPVDYGFPALLDGCAR
                     FEGLDPVDQRGVVRPQDGDGQDRNAFFDGEPIRVPADCDIGAVEAEAFVAPPTGPVLA
                     GPPFTG"
     gene            1082..2410
                     /locus_tag="VFP02_04320"
     CDS             1082..2410
                     /locus_tag="VFP02_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9542279.1"
                     /translation="MPEYVERMFPEPVDLGIAEDDRAVTNVPAGTRAHLEGAGGEATN
                     WYEGGGAIGDQAVKGITIYPPLRGIKTQGDPFEPVKIGILIDMELGQLLADWIDPTIL
                     AIEDALNEGIWRRGPIELVVADARALPRENYRKVIEGYRWLVEQGCVVVLGPMISDNC
                     LILQETVNELRVPSIGWTGAHKYASEYAFTIANGDIPSEGAMCAHWLKQQGHEKVGLF
                     WEQGSSGKDYCEFFRDTALSLGLTITREVKLEPNPVGLQDDLAAMRDLGTEGIYYGGY
                     GYATFHFADAFKALDWDPPRVMGTAFMFYSNSNAWAEGLEGWHGVDQLGEDGANPNYN
                     AMIERFEKRFGRVSRNVVVALAYDTARVAIHGIGNAAIPEPRWVKEGMERIRWMPATN
                     GGPGTYIQFGPHDRKGYKGDFLTIRHLRNGELEFNGYHRPEWPSNATVPS"
     gene            2407..3039
                     /locus_tag="VFP02_04325"
     CDS             2407..3039
                     /locus_tag="VFP02_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9542280.1"
                     /translation="MSSTTASRREERRQLHQDLSRTQLLDAAEEVFGAKGFHETTLKE
                     IAELAEFSVGSVYSFFENKDDLFLNVFLRRGEEFLPGMEAAAGVGDDARAQLHALVDY
                     EVGFFRAHPHFGRLYLRTSSSTLPGMELPPSQTLAANFERAMGVQAGIFRRGQAAGVL
                     RDGDPAVLGRLFTGLVAAYQSTDPAVVADDPRERLPLTALHDIVDRAFSP"
BASE COUNT          492 a         1122 c         1047 g          463 t
ORIGIN      
        1 catctggtca acatcaccct gcagaacggg agtgccgccg gcgacggcgg cgccgtcctc
       61 gtcttgaatg cctcgtccgt gcacgccgtc gatgctcggt tcgactcgaa cgaggcgtcg
      121 ggcagtggcg gcgccctgga gatcccgctc gaggccttcg tggcccgttc ggacttcacc
      181 ggcaacgtcg cggcgtccgg tgatggtggg gccctcgacg cggccgcggc cgccggccag
      241 ttccgaatcc gccagtcgca gttcgtcgag aaccacgcgc cggcgggcga gggcggagcg
      301 gtcagcgcca tcgggttcgg gtccatctcg acgggcgatg gtgtccgggg atcgtcgttt
      361 gtcgggaaca ccgcccgccg aggcggggcc ctggtgctgt ccagtgggat cggtgtcgcc
      421 aactcgacca tcgtcaacaa catggcgacg gtcggcggcg gcgggatcct ggccgagacc
      481 accagcgcca gcgccgggac ctacctgacc gtcacgggca atgcggcgcc ggacggggcc
      541 aatctcagct accgcggcca tgatctccac ctcttcttct ccgtgatcgc ggagccggaa
      601 ggtggcggtg agaactgtca catcaccgga tcgattcacc ccaacctcgt ccataccgac
      661 gaccacagct gtggacatca tcaagtcaac agtggtccac cccagctggg gccgctgacc
      721 gacaacggcg gccggaccct cacccgcgag cccctcgctg gtagcccgct cctcgatgcg
      781 ataccggtcg actacgggtt ccctgccctg ctcgacggct gcgcccgctt cgagggcctc
      841 gacccggtgg accagcgagg cgtggtgcgc ccgcaggacg gcgatggcca ggaccggaat
      901 gcgttcttcg acggcgagcc catccgggtc cccgccgact gcgacatcgg tgcggtcgag
      961 gccgaggcct tcgtggcccc gccgaccggg cccgtgctcg ccggtccgcc gttcacggga
     1021 tgacggcgct ccaagatctg aaccagcgtt catcgacttg aacctggact agtgtccggg
     1081 catgccggag tacgtggagc gcatgttccc cgagcccgtc gacctcggga tcgccgagga
     1141 cgaccgggcc gtcaccaacg tccccgccgg gacccgggcc cacctggagg gcgccggcgg
     1201 cgaggccacc aactggtacg agggcggcgg cgccatcggc gaccaggcgg tcaaggggat
     1261 caccatctat ccgcccctcc ggggcatcaa gacccagggc gaccccttcg agccggtgaa
     1321 gatcggcatc ctcatcgaca tggagctggg tcagctcctg gccgactgga tcgacccgac
     1381 catcctggcc atcgaggacg ccctcaacga gggcatctgg cggcggggcc cgatcgagct
     1441 ggtggtggcc gacgcccggg ccctcccccg ggagaactac cggaaggtca tcgagggcta
     1501 ccggtggctc gtcgagcagg ggtgcgtcgt cgtgctcggc ccgatgatct cggacaactg
     1561 cctcatcctg caggagacgg tcaacgagct gcgggtgccg tccatcggct ggaccggggc
     1621 ccacaagtac gcgtcggagt acgccttcac catcgcgaac ggcgacatcc cgtccgaggg
     1681 ggccatgtgc gcccactggc tcaagcagca gggccacgag aaggtcgggc tcttctggga
     1741 gcaggggtcg tcggggaagg actactgcga gttcttccgc gacaccgccc tcagcctcgg
     1801 cctgacgatc acccgtgagg tcaagctcga gcccaacccc gtcggcctgc aggacgacct
     1861 ggccgccatg cgcgacctcg ggacggaagg catctactac gggggctacg gctacgccac
     1921 gttccacttc gccgacgcct tcaaggccct cgattgggac ccgccccggg tcatgggcac
     1981 ggccttcatg ttctattcga actccaacgc ctgggccgag ggcctggagg gctggcacgg
     2041 cgtcgaccaa ctgggcgagg acggggccaa ccccaactac aacgccatga tcgagcgctt
     2101 cgagaagcgc ttcggccggg tcagccggaa cgtcgtcgtg gccctggcct acgacacggc
     2161 gcgggtggcc atccacggca tcggcaacgc ggccatcccc gagccccgtt gggtgaagga
     2221 gggcatggag cgcatccgct ggatgccggc caccaacggc gggcccggca cgtacatcca
     2281 gttcggtccc cacgaccgca agggctacaa gggcgacttc ctcaccatcc gccacctgcg
     2341 caacggtgag ctcgagttca acggctacca ccgccccgag tggccgtcga acgccacggt
     2401 cccgtcgtga gcagcaccac cgccagccgt cgcgaggagc gccggcagct ccaccaggac
     2461 ctgagccgca cccagctgct cgatgcggcc gaggaggtct tcggggccaa gggcttccac
     2521 gagaccaccc tgaaggagat cgccgagctg gcggagttct cggtgggctc ggtgtactcg
     2581 ttcttcgaga acaaggacga cctgttcctc aacgtcttcc tccggcgggg cgaggagttc
     2641 ctgcccggca tggaggcggc cgccggcgtc ggtgacgacg cccgggccca gctccacgcc
     2701 ctcgtcgact acgaggtcgg cttcttccgg gcccaccccc acttcggtcg gctctacttg
     2761 cgcacgtcca gctccacgct gccgggcatg gagttgccgc cgtcgcagac cctggccgcc
     2821 aacttcgagc gggccatggg cgtgcaggcc ggcatcttcc gccggggaca agccgccggt
     2881 gtccttcggg acggtgaccc ggcggtcctg ggccgtctgt tcaccggcct ggtcgccgcc
     2941 taccagtcga cggacccggc cgtcgtggcc gacgaccccc gcgagcgcct cccgctgacc
     3001 gccctccacg acatcgtcga ccgcgccttc agcccgtagc gaagttgcgc cagctggctg
     3061 cactttccgc aacggatcgg cgccagatcg tgggaagggc ggggacggtc aggtcagctc
     3121 gagc
//
LOCUS       DASTFV010000227         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_886913, whole genome shotgun sequence.
ACCESSION   DASTFV010000227 DASTFV010000000
VERSION     DASTFV010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886913"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VFP02_04330"
     CDS             complement(<1..828)
                     /locus_tag="VFP02_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9542281.1"
                     /translation="MALAPLPPIEPLPLADLVAGRGLVHLERLPARAARFGELATPLP
                     DLVWERLGVDGLWAHQAAAIDEVRAGRSVVLATGTASGKSLCYQAPIAEAALAPLRPS
                     TALCIFPTKALAQDQARALFKLDLPGLVPATYDGDSSTEERTWARGKATVLLTNPEML
                     HSALLPHHAKWSTYLGRLEHVVVDELHVLRGIFGTHVAHLLRRLRRVCNLYGSDPTFV
                     FCSATIGRPGRLASELCGLPVTEITDDASPKGERLVALWNPAVEAEVDIDLTDHDDGR
                     "
     gene            934..1284
                     /locus_tag="VFP02_04335"
     CDS             934..1284
                     /locus_tag="VFP02_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9542282.1"
                     /translation="MSRYEEVAVEDHDGWRVLRLAGEVDVATAPRLRDRLVQLITEGP
                     PRLVVDLSGLTFIDSMGLGALVSGLKRARALDGDLRLAGPTDHVAKVLSITRLDKAFL
                     VGDTVEAALAGSPG"
     gene            1281..1727
                     /locus_tag="VFP02_04340"
     CDS             1281..1727
                     /locus_tag="VFP02_04340"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9542283.1"
                     /translation="MIPLGEVVELEIPPTMEYLDLARVVVSAAASVDPAFPPSRVSDL
                     RLAVSELCANAIESHQRADNDEPLTIRCDLDDDRVTIEIEDHGGGFDPDALSPMPHAT
                     DPSRLERERGLGIPLVRTLADDVEFLVKDDGTTVRVVLFHPNGTGR"
     gene            complement(1868..>2121)
                     /locus_tag="VFP02_04345"
     CDS             complement(1868..>2121)
                     /locus_tag="VFP02_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9542284.1"
                     /translation="WLMDTVGNKGARIEVSAIKVAVPNMARRVIDRAIQAHGAGGIGP
                     DFGLSSMYAHVRTLRLADGPDEVHRMQLAKREIRKYEAN"
BASE COUNT          298 a          738 c          774 g          311 t
ORIGIN      
        1 ccggccgtcg tcgtggtccg tcaggtcgat gtccacctcc gcctccacgg cggggttcca
       61 cagggcgacg aggcgctcac ccttggggga ggcgtcgtcg gtgatctcgg tgacgggcag
      121 cccgcagagc tcggaggcca ggcgccccgg ccggccgatg gtggccgagc agaacacgaa
      181 cgtcgggtcc gagccgtaga ggttgcagac cctgcgcagg cggcgcagca ggtgcgccac
      241 gtgggtgccg aagatccccc gcaggacgtg gagctcgtcg accaccacgt gctccagccg
      301 gccgaggtag gtcgaccact tggcgtggtg gggcagcagg gcggagtgca gcatctcggg
      361 gttggtcagc aggacggttg ccttcccccg ggcccaggtc cgctcctccg tggaggagtc
      421 gccgtcgtag gtggcgggga cgaggccggg caggtccagc ttgaacaggg cgcgggcctg
      481 gtcctgggcg agcgccttgg tcgggaagat gcagagggcg gtgctgggtc gcagcggggc
      541 cagcgcggcc tcggcgatgg gggcctggta gcagagggac ttgcccgaag cggtgccggt
      601 cgcgagcacg accgaccgcc cggcccggac ctcgtcgatg gccgcagcct ggtgggccca
      661 cagcccgtcg acgccgagcc gttcccagac gaggtcgggc aacggcgtgg ccaactcgcc
      721 gaagcgggcc gctcgcgcgg gcagccgttc gaggtggacc aggccgcggc ccgccacgag
      781 gtcggccagc ggcaagggtt cgatcggcgg cagcggcgcg agcgccattg ctcgagccta
      841 cgaccgcgcg gtgacaggtg acaggggcgt aactacgacg gcggcattcg ggcattacgg
      901 atccatgacg gcagccgtcg tacgctggcg ggagtgagcc gctatgagga ggtggccgtc
      961 gaggaccacg acggctggcg cgtgctgcgc ctggccggcg aagtcgatgt cgccaccgcc
     1021 ccccgcctcc gggaccgcct cgtgcagctc atcaccgagg gcccaccccg gctcgtggtc
     1081 gacctgagcg ggctcacctt catcgactcg atgggcctgg gggcgctggt cagcggcctg
     1141 aagcgggccc gggccctgga cggcgacctg cgcctggccg ggcccaccga ccacgtggcc
     1201 aaggtcctgt ccatcacccg gctggacaag gccttcctgg tcggcgacac cgtcgaggcc
     1261 gctctggccg gaagcccggg gtgatcccgt tgggcgaggt cgtcgagctc gagatcccgc
     1321 ccaccatgga gtacctcgat ctcgcccgtg tcgtggtgag cgcggccgca tcggtcgacc
     1381 ccgccttccc cccgtcccgc gtctcggatc tgcgcctggc cgtgtccgag ctgtgtgcca
     1441 atgcgatcga gtcccaccag cgggcggaca acgacgagcc cctgaccatc cgctgtgacc
     1501 tcgacgacga ccgggtcacc atcgagatcg aggatcacgg cggcggcttc gaccccgacg
     1561 cgctgtcccc gatgccccac gccaccgatc cgtcccggct ggaacgggag cggggcctcg
     1621 gcatccctct ggtccggacc ctggccgacg acgtggagtt cctggtcaag gacgacggca
     1681 ccacggtccg cgtcgtcctc ttccacccga atgggacggg ccgctgacga gaagttcggg
     1741 tgaacctctc ccagtcgggt aaggggaggc gatgaacctg ctcctcgtca tcctcgtggt
     1801 cctcatcgtc ctcgccctga tcggcgtgat cggtcgcagt cgccgcgtct gacccgaccg
     1861 tgggcgttca gttcgcctcg tacttgcgga tctcgcgctt ggccaactgc atccgatgga
     1921 cctcgtcggg accgtcggcc aggcgcagcg tgcgcacgtg ggcgtacatg gacgagaggc
     1981 cgaagtcggg cccgatcccg ccggcaccgt gggcctggat ggcccggtcg atgacccggc
     2041 gggccatgtt gggaacggcc accttgatgg ccgacacctc gatgcgggcg cccttgttgc
     2101 ccaccgtgtc catcagccac g
//
LOCUS       DASTFV010000228         5287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_888395, whole genome shotgun sequence.
ACCESSION   DASTFV010000228 DASTFV010000000
VERSION     DASTFV010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888395"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VFP02_04350"
     CDS             complement(<1..562)
                     /locus_tag="VFP02_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542285.1"
                     /translation="MKIWSRREVLVAGAAAAVVAACGGDDGGTADATATAPTTTAPPA
                     GSAAPTTGATGATLTAADFDGLGTCALTPEQTAGPFPLEEQFERRDITEGYPGHRLHL
                     GLRVVDADCAPVAGAAVEVWHTDATGDYSAFTDNGGGRDEAEGTTFLRGTQTAGDDGI
                     VAFTTIYPGWYRGRAVHIHVRVHRDDA"
     gene            complement(574..1116)
                     /locus_tag="VFP02_04355"
     CDS             complement(574..1116)
                     /locus_tag="VFP02_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542286.1"
                     /translation="MRVAPFALFVAAATMVVGCGDDGGGATDAEAATGACGVLVAWTN
                     DVAEIVNDTQVAMQDGADFQALMLAAIDDVVARTDDLGTELAAVAYPDTEGGDALAEE
                     LARGQQAAAEDIAGFREEILAIPDPDPEGRNFRKAQLVVELEKPRSLVKPDVQGDLGD
                     EGLEAAIAAEDSCRFVTRSQ"
     gene            1170..2030
                     /locus_tag="VFP02_04360"
     CDS             1170..2030
                     /locus_tag="VFP02_04360"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106411 - XMP 5'-nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET9542287.1"
                     /translation="MRVRMTAAGLASVLVLAGCGGDDGGGGDDAASDTTAAPAEDTTT
                     TTAAPETFRILVSNDDGHEGVGEGLDTLVEALLTLEDVEVTVSIPLEDQSGTSDNTEP
                     GEQPTSEVTTLSGYPATAVDGFPADAVEQGLASMAEPPHLVVTGINYGQNIGPLTELS
                     GTVGAARTGGRAGIPSLAVSQGIFVNGGPEPDYPAAAELALAWVDEHRDELVAADPVE
                     GVAAVTNLNVPTCPSGDQGELLEVPVATEAEVAGLDLGAVDCETAVPDPTNDVTGFLA
                     GHPVQSDLTA"
     gene            2269..4425
                     /locus_tag="VFP02_04365"
     CDS             2269..4425
                     /locus_tag="VFP02_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9542288.1"
                     /translation="MLWGSLLLAWATGGTDHVAPHWFFVPVLLAGGRFGPWGVLTAGL
                     TAGLLAGPLTPADVDAAQAQTFSDWGTRTVFFVVVGLFFAHVLGRTRRSAHQELEGLR
                     LGSELAGAADRGELVLEYQPVVALRGREVIAAEALVRWEHPDRGRVMPDQFIPQSESS
                     GEIHPVGAWVIDEACRQFAVWRKGELDPDRPFRLSVNVSIHQVEDGDLAAVVERALRR
                     HRLPADGLVLELTETAILTESSDALWQLERLRAMGVLIAVDDFGTGYSSLAYLRRLPV
                     DIVKIDRAFVQRLGHDAHADDRRIAAGIIDLAHDLGLTVVAEGIETESQARTLLDLGC
                     HRGQGYLFAPPLTPDRLVDLIADERADPASTSTGARARPSLPKAAHRWFGRALRTKDT
                     TGQHEARTVALAALFIAGASMALTTSVLTPERTRDTVTAALVASIGIPTAILLLRFGT
                     WIPRWGLHVLLAGGTLVVSVGALLSDDPGLMVATTSLYIWVVLYAAAFYDWPATAGHF
                     ALVAACLTGVLTVTDTYQPVGILIMILGSSAVGAGVVGWLARQLRAVASTDLLTGLPN
                     RQAFEALLPREIARAERDGSSLCLAVVDVDDFKEINDTHGHQAGDGILATLAGQWQGV
                     LRATDVLARVGGDEFVVLLPGCQLADAVPVLERMGASGQPSCSVGVARLGEGETPDRL
                     MARADRALYRAKAAGTGLVVPDHVDAVDPETVATAR"
     gene            4436..5140
                     /locus_tag="VFP02_04370"
     CDS             4436..5140
                     /locus_tag="VFP02_04370"
                     /inference="COORDINATES: protein motif:HMM:NF004858.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HET9542289.1"
                     /translation="MSSILQYDLDGAVAVLRLDDGKANAIGPDTVAALDEALTRAETE
                     AGALLIIGREGKFSAGFDLSVMTSGVEAMQGLVRSGAELLVRLYASPLATVAACTGHA
                     LAMGSLLLLASDTRIGADGPFKLGLNEVAIGMGLPIFAVELARDRLSPRHFTAATVQG
                     QVFDPAGAVEAGYLDRLVPADDLEATARDEAGRLAQLRRGAVGHTKARARQATVDHIR
                     ATLAEDMAATGMPEPR"
BASE COUNT          687 a         2009 c         1860 g          731 t
ORIGIN      
        1 cggcatcgtc tcggtggacc cggacgtgga tgtgcacggc ccggccccgg taccagcccg
       61 ggtagatggt ggtgaacgcg acgatcccgt cgtcccccgc cgtctgggtg ccccgcagga
      121 acgtggtccc ctcggcctcg tccctgcccc cgccgttgtc ggtgaaggcc gagtagtccc
      181 cggtggcatc ggtgtgccac acctcgacgg cggcgcccgc aaccggcgcg caatcggcgt
      241 ccacgacccg gaggcccaag tggagccgat gccccgggta gccctcggtg atgtcccggc
      301 gctcgaactg ctcctcgagg gggaacgggc ccgccgtctg ctccggggtc aaggcgcagg
      361 tgccgagacc gtcgaagtcg gccgcggtca gcgtcgcccc cgtcgcccct gtggtcgggg
      421 cggcggagcc ggcgggcggc gctgtggtgg tgggtgccgt ggccgtggcg tccgccgtac
      481 caccgtcgtc gcccccgcag gcggccacga ccgcagctgc cgctccggcc accagcacct
      541 cgcgtcggga ccagatcttc atcgggtgcc tccctactgc gagcgggtga cgaagcggca
      601 cgagtcctcg gccgcgatgg cggcctcgag gccctcgtcg ccgaggtcac cctgcacgtc
      661 gggcttgacc agggaccggg gcttctccaa ctccacgacc agctgggcct tccggaagtt
      721 ccggccctcg gggtcgggat ccgggatggc caggatctcc tcccggaagc cggcaatgtc
      781 ctcggccgcc gcctgctggc cacgcgccag ctcctcggcg agggcgtcgc ccccctcggt
      841 gtcggggtag gccacggcgg ccagctcggt gcccaggtcg tcggtccggg ccaccacatc
      901 gtcgatggcg gcgagcatga gcgcctggaa gtcagccccg tcctgcatgg ccacctgggt
      961 gtcgttgacg atctcggcca cgtcgttggt ccaggccacg agcaccccgc aggcgccggt
     1021 cgccgcctcg gcgtccgtcg ccccaccccc gtcgtcccca cacccgacga ccatcgtggc
     1081 ggccgcgacg aagagagcga atggagcgac acgcacccgg ccgacgatac tgaccgtcct
     1141 cagatcacga ctcgggggag acggcacaga tgcgggtacg gatgacggcg gccggtttgg
     1201 cctcggtgtt ggtgctggcg ggctgcggcg gtgacgacgg gggcggcggt gacgacgccg
     1261 cgtcggacac caccgcggcg ccggcggagg acaccacgac cacgacggcg gcgccggaga
     1321 ccttccgcat cctcgtgtcc aacgacgacg gccacgaggg cgtaggagag ggcctcgaca
     1381 ccctggtcga ggcgctgctc acgctggagg acgtcgaggt cactgtctcg atcccgctgg
     1441 aagaccagag cggcaccagc gacaacaccg agccgggcga gcagcccacc agcgaggtga
     1501 cgacgctctc gggctatcca gcgaccgcgg tcgacggctt ccccgccgac gccgtcgagc
     1561 aggggctggc ctccatggcc gaaccaccgc acctcgtcgt caccggcatc aactacggcc
     1621 agaacatcgg ccccctcacc gagctgtcgg ggacggtggg cgccgcccgc accggcggcc
     1681 gggccggcat cccgtcgctg gccgtgagcc agggcatctt cgtcaacggc ggtcccgagc
     1741 cggactaccc ggcggcggcg gaactcgcgc tggcctgggt cgatgaacac cgcgatgagc
     1801 tggtggcggc cgacccggtc gagggcgtgg ccgccgtcac caacctcaac gtgcccacct
     1861 gcccgtcggg tgaccagggc gagttgctgg aggttccggt agccacggag gccgaggtcg
     1921 ccggactgga cctgggtgcc gtggactgcg agacggcagt gcccgacccc accaacgacg
     1981 tcaccggctt cctggccggc caccccgtcc agagcgacct caccgcctga tccccaagcc
     2041 cgtcgacacc cccgccgacc tcgaggcccc acctgtcgga tctgccaggt agggcctcgg
     2101 gaatgatcgg cgggggactc gcaagaatcg gcgggggcgg ccgacgggaa gggcatccct
     2161 gtctcctccg tacccgcccg gcctgctgag ggcgacgacc ggctcctccg cctcctggag
     2221 ggcgggccgg ggaccagccg cgccctgagc gcggcgtggg tcgccgcgtt gctctggggg
     2281 tccctcctgc tggcctgggc caccggaggc accgaccacg tcgccccgca ctggttcttc
     2341 gtccctgtcc tcctggccgg tgggcgcttc ggcccctggg gcgtcctcac cgccggcctg
     2401 acggcgggcc tcctggccgg gcccctgacc ccggccgacg tcgacgcggc gcaggcccag
     2461 acgttcagcg actgggggac ccggacggtg ttcttcgtcg tcgtgggcct cttcttcgcc
     2521 catgtgctcg gccgcacccg tcggtcggcc caccaggagc tggagggcct ccgactcggc
     2581 agcgagctgg ccggggccgc cgatcgcggc gagctggtgc tggagtacca gcccgtcgtg
     2641 gcgctgcgcg ggcgcgaggt gatcgccgcc gaagccctgg tgcgctggga gcatcccgac
     2701 cggggccggg tcatgcccga ccagttcatc ccccagagtg agtcctcggg ggagatccac
     2761 cccgtggggg cgtgggtgat cgacgaggcc tgtcggcagt tcgccgtgtg gcgcaagggg
     2821 gaactcgatc ctgaccggcc gttccgcctc tcggtcaacg tgtcgatcca ccaggtcgag
     2881 gacggtgacc tggccgccgt ggtcgagcgg gccctgcgcc gccatcgcct cccggcggac
     2941 ggcctcgtgc tggagctgac cgagaccgcc atcctgaccg agagctccga cgccctgtgg
     3001 cagctggaac gcctgcgggc catgggtgtg ctcatcgccg tcgacgactt cggcaccggc
     3061 tactcctcgc tggcctacct ccggcggcta cccgtcgaca tcgtgaagat cgaccgggcg
     3121 ttcgtgcaac gcctcggtca cgacgcccat gccgacgacc gccgcatcgc cgccgggatc
     3181 atcgacctgg cccacgacct cggcctgacc gtggtggcgg agggcatcga gaccgagagc
     3241 caggcccgca cactcctcga tctgggctgc caccggggcc agggctacct gttcgccccg
     3301 ccgctgacgc ccgaccgcct ggtggacctc atcgccgacg agcgcgccga cccggcctcg
     3361 acctcgaccg gggcccgggc gaggccgagc ctccccaagg ccgcccaccg ctggttcggc
     3421 cgggccctgc ggaccaagga cacgacgggc cagcacgagg cccgcaccgt ggccctggcc
     3481 gccctcttca tcgccggggc gtccatggcc ctgaccacgt cggtgctcac cccggagcgg
     3541 acccgcgaca cggtgaccgc ggccctggtg gcgtcgatcg gcatccccac cgcgatcctc
     3601 ctgctccgct tcggcacctg gatcccccgc tggggcctcc acgtgctcct ggcgggaggc
     3661 accctcgtcg tgtcggtggg cgccctgctc agcgacgatc ccggcctcat ggtggccacg
     3721 accagcctct acatctgggt cgtcctctac gccgccgcct tctacgactg gccggcgacg
     3781 gccggtcact tcgccctcgt ggccgcgtgc ctgaccggtg tgctgacggt gaccgacacc
     3841 taccaaccgg tcgggatcct catcatgatc ctcggctcct cggcggtcgg tgccggggtg
     3901 gtgggttggc tggcccgcca gctgcgagcg gtggcctcga ccgacctgct caccgggctg
     3961 cccaaccggc aggccttcga ggccctgctg ccccgggaga tcgctcgggc tgaacgggac
     4021 ggctcgtcac tctgcctcgc cgtcgtcgac gtcgacgact tcaaggagat caacgacacc
     4081 cacggccacc aggccggtga cggcatcctg gccaccctgg ccggccagtg gcagggggtg
     4141 ctccgggcga ccgacgtgct ggcccgcgtc gggggcgacg agttcgtcgt gctcctgccc
     4201 ggatgccagc tggccgacgc cgtccccgtc ctcgaacgca tgggcgcgag tggccagccg
     4261 tcgtgctcgg tcggggtggc gaggctcggc gagggcgaga ccccggaccg cctcatggcc
     4321 cgggccgacc gggccctcta ccgggccaaa gcggcgggca cgggcctcgt cgtgcccgat
     4381 cacgtcgacg ccgtcgatcc ggagacggtg gccaccgctc ggtagcgtca gccgggtgag
     4441 cagcatcctc cagtacgacc tcgacggcgc ggtggccgtc ctccgcctcg acgacggcaa
     4501 ggccaacgcc atcgggccgg acacggtcgc cgccctcgac gaggcgctga cccgggccga
     4561 gaccgaggcg ggcgccctgc tcatcatcgg tcgtgagggc aagttctcgg ccggcttcga
     4621 cctgtcggtc atgacctcgg gcgtggaggc gatgcagggc ctggttcgct ccggtgccga
     4681 gctgttggtc cgcctctacg ccagccccct ggcgaccgtg gccgcctgca ccggccacgc
     4741 tctggcgatg ggcagcctcc tgctcctggc cagcgacacc cgcatcgggg ccgacggccc
     4801 cttcaagctg ggcctgaacg aggtggccat cggcatgggc ctgcccatct tcgccgtgga
     4861 gctggcccgg gaccgcctgt cgccccggca cttcaccgcc gccaccgtcc aggggcaggt
     4921 cttcgacccg gccggggcgg tcgaggccgg ctacctggac cgcctcgtcc ccgcggacga
     4981 cctggaggcc accgcccgcg acgaggccgg tcgcctggcc cagctccggc ggggcgcggt
     5041 cggccacacc aaggcccggg cccggcaggc cacggtcgac cacatccggg cgaccctggc
     5101 tgaggacatg gccgccaccg gcatgcctga accccgctga attccctcgc ctcaccccac
     5161 cgggaagggc aggctgtcgg ggtggcgtac ctgacgaccg aagagctcga cgccggcctg
     5221 gacgacatcc gtcggtcgcc gagggacgga ggcaaggtcg tgctgatcgt ggcccgaccc
     5281 gaccccg
//
LOCUS       DASTFV010000229         4310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_892975, whole genome shotgun sequence.
ACCESSION   DASTFV010000229 DASTFV010000000
VERSION     DASTFV010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892975"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..699
                     /locus_tag="VFP02_04375"
     CDS             133..699
                     /locus_tag="VFP02_04375"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET9542290.1"
                     /translation="MSRTRLIVFGRQGAGKGTQCSRLAGHYGIPHISTGDMLREAVAE
                     GTELGLKAKVLMDAGDLVPDDVMEGIVVERLAKPDAEPGWLLDGYPRTLGQVEAMVRH
                     LGEGFVDLAVNLDVPVDVVTERMVARGRDDDTEEGIRRRLELYEEQTAPLLSWFRAKD
                     LLVDVDGVGTEDEVSERLVKVVDAALEA"
     gene            701..1459
                     /gene="map"
                     /locus_tag="VFP02_04380"
     CDS             701..1459
                     /gene="map"
                     /locus_tag="VFP02_04380"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET9542291.1"
                     /translation="MRRSADELAKMRRAGRVVAEMHDKIRAAIRPGVTTRQLDAIGRE
                     VIERNGARSNFLNYGGGPGAPPFPAVICASPNDVIVHGIPDDTVLVEGDIISIDCGAI
                     IEGYHGDAAFTAPVGQVTDEAARLLEVTEASLFAGIEAMVDGNRLSDIGHAVQTVAEA
                     AGYGVVREYTGHAIGTAMHESPQVPNWGKPGKGPKLRPGNVFAIEPMVNIGTAETRVM
                     ADGWGVRTADGSLSAHFEHSIAVTDNGPEILTLP"
     gene            1588..2442
                     /locus_tag="VFP02_04385"
                     /pseudo
     CDS             1588..2442
                     /locus_tag="VFP02_04385"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
     gene            complement(2725..3993)
                     /locus_tag="VFP02_04390"
     CDS             complement(2725..3993)
                     /locus_tag="VFP02_04390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9542292.1"
                     /translation="MNTVEQDDHPTLTASDRRGVAVTAVIAVTLLGAAPRLFRVWRRP
                     MGFAWDESYYVPAARSYLDGVFDVNLEHPPLGKWLIAAGIQLIGDEPLGWRAASLVAG
                     IVTVPLVWFLARRLLGSTAWATAAAFLVAIDGLLIVQSRTAVLDSLLPPLIVGATLAV
                     LVHLDRGYDRRRLSGPLLIAGGLLGAAVACKWEALPALLGAIGLWVLPPGRDRATLRT
                     TAIAFVAVPSGVYLAAHAGPLIQSGFDLPAFFRTQIDAVNYHRKFRANHERSSPAWTW
                     LWLRTPPSYISESGNGRVAHLVALGNPVLWWGFLACLPHLVFVWWHRRDRTVEVVLFA
                     LLTLYLPWLVILRPGFLYYLTPLVPFMAIGLTYSLRAVWDRGGALRLVPGGVLLAAFL
                     AFVAFVPIWTFSTIPQTRFDVLTPFADWGD"
BASE COUNT          616 a         1521 c         1543 g          630 t
ORIGIN      
        1 cctgcctgat cagagcacgt cgatcctgtc gttctccggc gtgtccgtgc tgatcggcgt
       61 cggtgtcgcc ctggagacga tgaagcagat cgacagccag ctgatgatgc gcaactacga
      121 gggcttcctc tcgtgagccg gacccgtctg atcgtcttcg ggcgccaggg ggcgggcaag
      181 ggaacacagt gctcgcggct ggccggccat tacgggatcc cgcacatctc gaccggcgac
      241 atgctccgtg aggccgtggc cgagggcacc gagctgggcc tcaaggccaa ggtcctcatg
      301 gacgccgggg acctggtccc cgacgacgtc atggagggga tcgtggtcga gcgcctggcc
      361 aagcccgatg ccgagccggg ttggctgctg gacggctacc cccgcaccct gggtcaggtc
      421 gaggccatgg ttcgccacct cggtgagggc ttcgtggacc tggccgtgaa cctcgacgtc
      481 cccgtcgatg tggtcaccga gcgcatggtc gcccggggtc gggacgacga caccgaggag
      541 gggatccgtc gccgcctcga gctctacgag gagcagacgg ccccgctcct gtcctggttc
      601 cgcgccaagg acctgctggt cgacgtcgac ggcgtcggca ccgaggacga ggtctccgag
      661 cgcctggtga aggtcgtcga cgcggccctg gaggcctgag gtgcgccgct ccgccgacga
      721 gctggccaag atgcgccgcg ccggtcgcgt cgtcgccgag atgcacgaca agatccgagc
      781 cgccatccgg cccggcgtca ccacccggca gctggacgcc atcggccgcg aggtcatcga
      841 gcgcaacggg gcccgttcca acttcctgaa ctacggcggc ggcccgggcg ctccgccgtt
      901 ccccgccgtc atctgcgcct cgcccaacga cgtgatcgtg cacgggatcc ctgacgacac
      961 ggtgctggtg gagggcgaca tcatctccat cgactgcggg gcgatcatcg agggctacca
     1021 cggcgacgcc gccttcaccg cccccgtcgg ccaggtcacc gacgaggcgg cccggctgct
     1081 cgaggtgacc gaggcgagcc tgttcgccgg catcgaggcc atggtcgacg gcaaccgcct
     1141 gtccgacatc ggccacgccg tgcagaccgt ggccgaggcc gcggggtacg gggtggtgcg
     1201 ggagtacacg ggccacgcca tcggcacggc catgcacgag agcccgcagg tccccaactg
     1261 gggcaagccg ggcaagggcc ccaagcttcg tcccggcaac gtcttcgcca tcgagcccat
     1321 ggtcaacatc ggcacggccg agacccgggt catggccgac ggttggggcg tgcgcaccgc
     1381 cgacggcagc ctctcggccc acttcgagca ctccatcgcc gtcaccgaca acggccccga
     1441 gatcctcacc cttccgtaga gctttcggtg gcggacccgt cgtccccacc tcgctcgtcc
     1501 ctcgagacgc ccggcgtcga accgcggcgt tcgccgtggg tggtgctgct cgtggtcatc
     1561 gtggcgttcg tcctgacccg gggcgcaatg gcgtacgtcg tcgagaaccc ggacctctac
     1621 tcggtcggac agcacgaggt catcgacgcc ggcttcgacc ggggcaacta ccggatcctg
     1681 agcgaacggt tccgggaggt cggggaccgg ccctatcagg actacccgct ggagtacccg
     1741 cccggggcct ttgcggccgc cctcgtccct gacctgttcg gccagccctt cgactacgag
     1801 ttccggttca tgctcttcgc cattgccgtc gacgccatcg gcctggtggc ggtgtggcgc
     1861 ctggcccggc ggaccggcgt gtggtggggg gtcggggcct ggatcgtgct cgtgccgctg
     1921 gtcggtccgg tgacctacag ccgactggac atcgtggtcg ccgcggccct ggcgtgggcc
     1981 ctcgagcggg cggtggcggg ggcctggggg aggagtggcg tcgccttcgg cgtcggcgcg
     2041 gtgagcaagc tggttcccgc gctgctgctg cccgccgccg tcctcgtggc accccgtcgg
     2101 tggcgtctcg tcgtcggggt cgccgccgtc gtcgccctcg ccgtcctccc cttcaccggc
     2161 cacctcggtg acctctatca cgacgtgatc acctatcacc gcgcccggcc ggtgcacgcc
     2221 gagagcctct gggcgtcgct ggccttcctc ctggaccgcc tcggcctggc cgcccctggc
     2281 atcgagttct cctacggcgc cttcaacgtc accggcgagt gggcggcggc cttccgcggc
     2341 ttggccgacc tcgccgccct cggcgtgctc gtcgactcgg tgctgcaggc caagctccga
     2401 gtgcgccggg gcgacggcgc ccacctggcc gtcctctcgg tggccacgct gaccctgctc
     2461 accgcggtcg gtcgggtgct gtcgcctcag tacgtcgtct ggctggtggc cctccaggcc
     2521 atcgggctct gcgtggcgcc ccgagccctg gcctggtccg cggcgtggat ggccctcgcc
     2581 gtcggcctga cccacctcga gtaccccgtc ttcttcggcg atgtcatgaa ccgccacgct
     2641 gaggcggagt acctgctgct ggcccgcaac gtcgccatcc tcgtgtcggg cctcctggcc
     2701 gcccgggtct cgtggcggtt ccggtcagtc gccccagtcg gcgaaggggg tcaggacgtc
     2761 gaagcgcgtc tggggaatgg tggagaaggt ccagatgggc acgaaggcga cgaaggccag
     2821 gaacgcggcg aggaggacgc cgcccggaac caggcgtagc gccccacccc ggtcccagac
     2881 ggcgcggagg ctgtaggtca ggccgatggc catgaacggg acgagggggg tgaggtagta
     2941 gaggaagccc ggccgcagga tcaccagcca gggcaggtac agggtgagga gggcgaagag
     3001 cacgacctcc accgtccggt cccgtcggtg ccaccagacg aacacgaggt gggggaggca
     3061 ggccaggaag ccccaccaca ggaccgggtt gccgagggcc acgaggtggg caacccggcc
     3121 gttccccgat tccgagatgt aactgggcgg ggtccgcagc cagagccagg tccaggccgg
     3181 gctcgaccgt tcgtggttgg cccggaactt ccggtggtag ttgacggcgt cgatctgggt
     3241 ccggaagaac gccggaaggt cgaatccgga ctggatgagc ggcccggcgt gggcggcgag
     3301 gtagaccccg ctgggtacgg ccacgaaggc gatcgcggtc gtgcgcagcg tggcccggtc
     3361 ccgacccggg gggagaaccc agaggccgat ggccccgagc agggcgggca gcgcctccca
     3421 cttgcaggcc acggcggccc cgagcaggcc gccggcgatg aggaggggcc cgctcagccg
     3481 acggcggtcg tagccccggt cgaggtggac gagcaccgcc agcgtcgccc cgacgatgag
     3541 cgggggcagc aggctgtcga ggaccgcggt ccggctctgg acgatgagca ggccgtcgat
     3601 cgcgacgagg aacgcggccg cagtggccca cgccgtggag ccgagcaggc ggcgggccag
     3661 gaaccagacc aggggcacgg tgacgatgcc ggccaccagt gacgccgccc gccagcccag
     3721 gggctcgtcc ccgatgagtt ggatgccggc ggcgatcagc cacttgccca gtggcgggtg
     3781 ttcgaggttc acgtcgaaca ccccgtcgag gtacgagcgg gcggcgggca cgtagtagga
     3841 ctcgtcccag gcgaagccca tcgggcgccg ccagacccgg aacaggcggg gcgccgcccc
     3901 caggagggtg accgcgatca ccgccgtcac ggcgacccca cggcggtcgg aggccgtgag
     3961 ggtggggtgg tcgtcctgct cgacggtgtt catctcgtcc cggtccggct gctaccatcc
     4021 ggagttcacc ggacgaaaaa acgaaggaac tcccccctat gcgctgggca tctcgaaggg
     4081 tagcggcgac cctactcgcc accggagtca tctttgtcgg gctgggcgcc tcggcccagg
     4141 ccgcggtgaa cgaccagatc ccggcggccg agcccggcgc cgagggcacg gtgtcctcga
     4201 gttcgacctc gacctcgacc tcgtcgacgg acacgtccac ctcgtccatc tcctccacgt
     4261 ccaccgacac gagctcgacc gtctcgtcga cgtcggtcag cacttcgtcc
//
LOCUS       DASTFV010000230         3537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_900857, whole genome shotgun sequence.
ACCESSION   DASTFV010000230 DASTFV010000000
VERSION     DASTFV010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900857"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1558)
                     /locus_tag="VFP02_04395"
     CDS             complement(86..1558)
                     /locus_tag="VFP02_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9542293.1"
                     /translation="MTDTASPATTGDALVDLLNGFIAELREAGLPVSLTENLDAMQAV
                     RHIPLEDREAFKYALAATLVKNNAHWRAFETVFEVYFSYRGSHYTIVDDDGELLSDEE
                     LAEMGEMPGAEGGQGGAGEAMSNEELAEMLYRALMNGDEALMRALARQSVSRFAGMEP
                     GRPVGGTYYLYRTLRNLDLDGMLDRLMEQAQQAAPEPMTPLEARLEADEYQTRIDQLK
                     KEIEAEIRRRLVADRGVEAMAKTLRKPLPEDVDFMHASREEMADLRKAIYPLTRKLAV
                     RLTRKRRHGRKGPLDFRNTVRHSLSYGGVPAEPKFRYPKPSKPEIFVIADISGSVAAF
                     ARFTLHLVYAISHQFSKVRAFVFIDGIDEVTRFFEGVEDIGDAIHRVNTEADVVWVDG
                     HSDYGHAFEVFWNTYGKDVGPKTTVLILGDARNNYHASQSWVVKEIQHKARHVYWLNP
                     EPKSYWDTGDSIVGEYSIHTDGAYECRNLRQLETFVEKLA"
     gene            complement(1555..2463)
                     /locus_tag="VFP02_04400"
     CDS             complement(1555..2463)
                     /locus_tag="VFP02_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9542294.1"
                     /translation="MTYRFDAVDEVREGLSKVDYLADEGIAGVVYLADRLQKPILVEG
                     PAGTGKTQLAKSVAEMTGARLIRLQCYEGLDEAKALYEWNYKKQLLRIQAERNSEGTW
                     NEIEDDIFSDEFLLERPLLEAIRSPDPVVLLIDEVDRVEVETEALLLEILSDYQVSIP
                     ELGTVVATQIPLVFLTSNNTRELSEALKRRCLYLHIDYPDLEREKEIVLTKVPGVSEN
                     LADQIARIVRSIRQLELKKAPSVSETLDWARTLVHLGVEQVDEQQATETITILLKYQA
                     DIAKAIKEFGGDGQAWVKGGQAPSRS"
     gene            complement(2515..>3537)
                     /locus_tag="VFP02_04405"
     CDS             complement(2515..>3537)
                     /locus_tag="VFP02_04405"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET9542295.1"
                     /translation="RIEAMAAGLRQVASLPDPVGEVLEGWTRPNGLRIHRVRVPLGVV
                     AIIYENRPNVTSDAAGLCVKSGNAAFLRGSSGAIDSNIAIAAVLREGLAKAGLPEDAV
                     VLVEDTSREAAVEFMRQRDSIDCLIPRGGPSLIQSILDNATVPYVIDGDGNCHVYVHE
                     DADLDMARAIIVNGKTQRPSVCNATESLLVHAAVADRLLPEAAKALEGVELVGDERVR
                     ALVPTAGEATDDDWAQEFLDLKLAVRVVDDLDEAIAHIARYGSGHSEAIVTDSVVAAD
                     RFCQEVDAAAVLVNASTRFVDGEEFGFGAEIGISTQKLHARGPMGLRELTTAKYVVRG
                     TGQTRA"
BASE COUNT          517 a         1182 c         1228 g          610 t
ORIGIN      
        1 agcgccgggc gaaccacgcg gaccagccgg tgacggtcga gggagcgtcc tcggcctcga
       61 cctcagcctc ggtcatgagc tgccgtcagg ccagcttttc gacgaaggtc tcgagctggc
      121 ggaggttgcg gcactcgtag gcgccgtcgg tgtggatgct gtactccccc acgatggagt
      181 cgcccgtgtc ccagtacgac ttcggctcgg ggttcagcca gtagacgtgc cgagccttgt
      241 gctggatctc cttgacgacc caggactggg aggcgtggta gttgttccga gcgtcgccca
      301 ggatcaggac cgtggtcttg ggccccacgt ccttgccgta ggtgttccag aacacctcga
      361 aggcgtggcc gtagtcggag tgcccgtcga cccagaccac gtcggcctcg gtgttcaccc
      421 ggtggatggc atcgccgatg tcctccaccc cctcgaagaa gcgagtgacc tcgtcgatgc
      481 cgtcgatgaa gacgaaggcc cggaccttcg agaactggtg gctgatggcg tagacgaggt
      541 gcagggtgaa gcgggcgaag gcggcgaccg agccggagat gtcggcgatg acgaagatct
      601 cgggctttga cggcttcggg tagcggaact tgggctcagc cggcacgccc ccgtagctca
      661 gcgagtggcg gacggtgttg cggaagtcca gcgggccctt gcgcccgtgg cggcgcttgc
      721 gggtcaggcg gacggcgagc ttgcgggtca gcgggtagat ggccttgcgc aggtcggcca
      781 tctcctcccg gctggcgtgc atgaagtcga cgtcctcggg cagcggcttg cgcagcgtct
      841 tggccatggc ctccacgccc cggtcggcga cgaggcgacg gcggatctcg gcctcgatct
      901 ccttcttcag ctggtcgatc cgggtctggt actcgtccgc ctccaggcgg gcctcgagcg
      961 gcgtcatcgg ctcgggcgcg gcctgctggg cctgctccat gagccggtcg agcatcccgt
     1021 cgaggtccag gttccgcagc gtgcggtaga ggtagtaggt cccgccgacc ggccggccgg
     1081 gctccatgcc ggcgaagcgg ctgaccgact gccgggccag cgcccgcatc agggcttcgt
     1141 cgccgttcat cagggcccgg tacagcatct cggccagttc ctcgttggac atggcctcgc
     1201 cggcgccacc ctggccgccc tccgcgccgg gcatctcgcc catctccgcc agttcctcgt
     1261 cggagaggag ctcaccgtcg tcgtcgacga tcgtgtagtg cgagccccgg taggagaagt
     1321 agacctcgaa gaccgtctca aaggcccgcc agtgggcgtt gttcttcacc aacgtggccg
     1381 ccagcgcgta cttgaacgcc tcgcggtcct ccagcgggat gtggcggacg gcctgcatgg
     1441 cgtccaggtt ctcggtcagg ctgaccggca acccggcctc gcgcagctcg gcgatgaagc
     1501 cgttgagcag gtcgaccagt gcgtcaccgg tcgtcgccgg cgacgcggtg tccgtcacga
     1561 gcggctcggc gcttgcccac ccttgaccca ggcctggccg tcaccgccga actccttgat
     1621 ggccttggcg atgtcggcct ggtacttgag caggatggtg atggtctcgg tggcctgctg
     1681 ctcgtcgacc tgctcgaccc cgagatggac cagcgtgcgg gcccagtcca gggtctcgga
     1741 gaccgagggc gccttcttga gctccaactg gcggatggag cgcacgatgc gagcgatctg
     1801 gtcggccagg ttctcgctga cgcccggcac cttggtcagc acgatctcct tctcccgttc
     1861 caggtcgggg tagtcgatgt gcagatacag acagcgccgc ttcagcgcct ccgagagctc
     1921 acgggtgttg ttggacgtga ggaagaccag cgggatctgg gtggccacga cggtgcccag
     1981 ctcggggatg gacacctggt agtcggacag gatctccagg agcagggcct cggtctcgac
     2041 ctcgacccga tccacctcgt cgatgaggag cacgacgggg tccggcgagc ggatcgcctc
     2101 caggagcggc cgttccagca ggaactcgtc ggagaagatg tcgtcctcga tctcgttcca
     2161 ggtgccttcg ctgttgcgct cggcctggat gcgcaggagc tgcttcttgt agttccactc
     2221 atagagcgcc ttggcctcgt ccagtccctc gtagcactgg aggcggatga ggcgggcccc
     2281 ggtcatctcg gccacggact tggccagctg ggtcttgccc gtgcccgcgg gcccctcgac
     2341 caggatcggc ttctgcaacc ggtcggcgag gtagacgacc ccggcgatgc cctcgtcggc
     2401 caggtagtcg accttgctca ggccttcgcg gacctcgtcc accgcgtcga agcgatatgt
     2461 catgggtcga gcctaccgat cgcacttgac cgcctggtca agattccgga gtcgtcaggc
     2521 tcgggtctga ccggtaccgc gcacgacgta cttggccgtg gtcaactccc ggaggcccat
     2581 ggggccacgg gcgtggagct tctgggtgga gatgccgatc tcggctccga agccgaactc
     2641 ctcgccgtcc acgaatcggg tcgaggcgtt gaccagcacg gcggcggcgt cgacctcctg
     2701 gcagaagcga tcggccgcga ccaccgagtc ggtgacgatc gcctcgctgt gtcccgagcc
     2761 gtaacgggcg atgtgggcga tggcctcgtc gaggtcgtcc accacgcgga cggcgagctt
     2821 gagatcgagg aactcctggg cccagtcgtc gtcggtggcc tcgcccgccg tcggcacgag
     2881 ggcccgcacc cgctcgtcgc ccaccagctc gaccccctcg agggccttgg ccgcctccgg
     2941 cagcaagcgg tcggccacgg cggcatgcac caacaggctc tcggtggcgt tgcagaccga
     3001 cggccgctgg gtcttgccgt tcacgatgat cgcccgagcc atgtccaggt cggcgtcctc
     3061 gtggacatag acgtggcagt tcccgtcccc gtcgatcacg tagggcacgg tggcgttgtc
     3121 caggatcgac tggatgagcg acgggccacc ccgggggatc agacagtcga tcgagtcacg
     3181 ctggcgcatg aactcgaccg cggcctccct gctggtgtcc tcgaccagga cgacggcgtc
     3241 ctcgggcagg ccggccttgg ccaggccctc ccgaaggacg gcggcgatgg cgatgttgga
     3301 gtcgatcgcc cccgacgagc cccgcaggaa ggcggcgttg cccgacttca cacacaggcc
     3361 ggcggcgtca ctggtcacgt tgggccggtt ctcgtagatg atggccacga cccccaaggg
     3421 gacacggacc cgatggatgc gcaggccgtt gggtcgcgtc cagccctcga ggacctcccc
     3481 taccgggtcc ggcagcgagg ccacctggcg caggccggcg gccatggcct cgatgcg
//
LOCUS       DASTFV010000231         2966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_902532, whole genome shotgun sequence.
ACCESSION   DASTFV010000231 DASTFV010000000
VERSION     DASTFV010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902532"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VFP02_04410"
     CDS             complement(<1..602)
                     /locus_tag="VFP02_04410"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9542296.1"
                     /translation="MATAIDCLVNVGFADEMQPEWMVRVKEDYFKGDDSFFQSPELPE
                     LLENMDANGVRRAVLMTRIGAVDDRAQRYAAAEPDRFALAVGGLNLLKPMKALRGLES
                     FVRDNPVAYTVVGPSFWGDGLYPPGDAVYYPLYTKCCELDLPLCMNTGLPGPPIPGEV
                     QNPIHLDRVCVRFPELRLCMIHGADPWWDVAIRLMLKYRNL"
     gene            complement(617..1408)
                     /locus_tag="VFP02_04415"
     CDS             complement(617..1408)
                     /locus_tag="VFP02_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET9542297.1"
                     /translation="MSDDVLVERSDGLVTVTFNRPERKNALDAGCWQALDAVLAEVAT
                     SPADRALLLTGAGGNFSSGADLSGDGSGGLAGGEMQLIVTEMRIVGEIIRRLQRLPKP
                     TLAAVDGFAVGVAFGLALACDLVVASDRARFCAIFAKRGLALDGGSSWTLPRQIGLRR
                     AKQMAYFGDMVEAPQALDWGLVNEVVPAEELAETARAWARRLADGPTTALSLSKRLLD
                     ASAGSSFEEALEGEARSQHITYTTADMGEGISAFLERRQPRFTGK"
     gene            complement(1450..>2966)
                     /locus_tag="VFP02_04420"
     CDS             complement(1450..>2966)
                     /locus_tag="VFP02_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HET9542298.1"
                     /translation="GDAETIPALLALRRRTAPDHPALVTEDAAVTYLDLDDASADLAA
                     RLVGDGMAKRDRVALLAPNGIDWAVIAYAVLRVGAVLVPLSTLLRPPELLAQLTTASV
                     SHLLTVPTFRGRSYLEDLEAAVPGLLEAVGSGARRPSAPDLRRVWTIPGGLPDRGVPP
                     AAVRAMEDRVRPADDLVVLFTSGSTGRPKGTIHTHAGGLRATAAGLGARGIGPGERLY
                     IPMPFFWTGGFSGGLLSTLLAGATLLTEAEPEPDRTLRLLERERVTVFRGWPDQAAKL
                     AAHPAFAAADLSSLGDASLGAVLPAERRPAPGARANLFGMTETFGPYCGDRLDTDLPP
                     DEWGSCGRPFAGIEVTIAEDGEIFVRGPNLMRGVCGRSRAEVFTPEGWFRTGDRGRID
                     DAGYLWYEGRLDDMFKVKGASVYPSEVEAAVRAVDDVRQAHVTEVDGDVGALVVTALP
                     VDAVAAATKERLSAFKRPTRWLVTADASEVPLTATGKIDKAALQTMLAEGATKL"
BASE COUNT          429 a         1072 c         1063 g          402 t
ORIGIN      
        1 aggttccggt acttcagcat caggcgaatg gccacgtccc accaggggtc ggccccgtgg
       61 atcatgcaca ggcgcagctc agggaagcgg acacagaccc gatccaggtg gatggggttc
      121 tgcacctcac cggggatcgg cggcccgggc aggccggtgt tcatgcagag cggcaggtcg
      181 agctcgcagc acttcgtgta gagggggtag tagacggcgt cgccgggcgg gtacaggccg
      241 tcgccccaga agctgggtcc gaccacggtg tacgccaccg ggttgtcgcg cacgaaggac
      301 tcgagccccc gcagcgcctt catcggcttg aggaggttga gcccgccgac ggccagcgcg
      361 aagcgatcgg gctcggccgc tgcatagcgc tgggcccggt cgtcgaccgc tccgatcctg
      421 gtcatcagga ccgcccgcct gaccccgttg gcgtccatgt tctcgaggag ctcgggcagc
      481 tcggggctct ggaagaacga gtcgtcgccc ttgaagtagt cctccttcac ccggaccatc
      541 cactcgggct gcatctcgtc ggcgaagccc acgttcacca ggcagtcgat cgcggtcgcc
      601 atcggcctct cctccgtcat ttcccggtga agcggggctg gcgccgctcg aggaaggcgc
      661 tgatgccctc gcccatgtcc gccgtcgtgt aggtgatgtg ttgggaccgg gcctcgccct
      721 ccagcgcctc ctcgaagctc gaccccgcgc tggcgtcgag cagccgcttg ctgaggctga
      781 gggccgtggt cggaccgtcg gccagccggc gggcccacgc ccgcgcggtc tcggccagct
      841 cctcggcggg caccacctcg ttcaccaggc cccagtcgag cgcctgcggg gcctcgacca
      901 tgtccccgaa gtacgccatc tgcttggccc gccggagccc gatctggcga gggagcgtcc
      961 aggaggaccc gccgtcgagg gccaagcccc gcttggcgaa gatcgcacag aagcgcgccc
     1021 ggtcgctggc caccaccagg tcacaggcga gggccaggcc gaaggcgaca ccgaccgcga
     1081 acccgtcgac ggcggccagc gtcggcttcg ggagccgctg gagccggcgg atgatctcgc
     1141 ccacgatgcg catctcggtc acgatcagct gcatctcacc gccggccagc cctcccgacc
     1201 cgtcgccgga caggtccgct ccggaggaga agttgccacc ggcgccggtc aggagcagcg
     1261 cccggtccgc ggggctggtc gccacctcgg cgagcaccgc gtcgagggcc tgccagcagc
     1321 cggcgtccag cgcgttcttc cgctccggac ggttgaaggt gacggtgacc aagccgtcgc
     1381 tgcgctcgac caggacgtcg tcggacatcg gcacacactc ctcccggaac gctcatggcg
     1441 gcaattcctc tagagctttg tagcaccctc ggccagcatc gtctgcagcg cagccttgtc
     1501 gatcttgccg gtggccgtca ggggaacctc gctggcatcg gcggtgacca gccaccgagt
     1561 gggcctcttg aacgcgctca gtcgctcctt cgtggccgcg gcgacggcgt cgaccgggag
     1621 ggccgtgacg acgagtgcgc cgacatcacc gtcgacctcg gtgacatgag cctggcggac
     1681 gtcgtcgacg gcgcgcacgg cggcctcgac ttcggacggg tacaccgagg cccccttgac
     1741 cttgaacatg tcgtcgaggc gaccctcgta ccagaggtac ccggcgtcat cgatccggcc
     1801 ccggtcgccg gtccggaacc agccctccgg ggtgaacacc tcagcgcgcg accgcccgca
     1861 cacgccgcgc atgaggttcg ggccccggac gaagatctcg ccgtcctccg cgatcgtcac
     1921 ctcgatgccg gcgaacggcc gcccgcagct cccccactcg tcgggcggga ggtcggtgtc
     1981 gaggcggtcc ccgcagtacg ggccgaaggt ctcggtcatc ccgaagaggt tcgcccgggc
     2041 ccccggagcc ggccgtcgct cggccggcag cacggcgcca aggctggcgt cgcccagcga
     2101 cgagagatcg gcggcggcga aggccgggtg ggccgccagc ttggcggcct ggtccggcca
     2161 accccggaac acggtcaccc gctcccgctc gaggagccgg agggtgcggt cgggctccgg
     2221 ttcggcctcg gtcagaaggg tggcccccgc cagcagcgtc gacagcagcc cgccgctgaa
     2281 ccccccggtc cagaagaagg gcatggggat gtagagacgc tcgccggggc ctatgccccg
     2341 ggcccccagc ccggcggccg tggcccgcag gccgccggcg tgggtgtgga tcgtgccttt
     2401 ggggcggccc gtgctccccg aggtgaacag cacgacgagg tcgtccgccg gccgcacccg
     2461 gtcctccatg gcgcggaccg cggcaggcgg gacgcctcgg tcggggagcc ccccggggat
     2521 ggtccacacc cggcgcaggt ccggcgcaga cggacggcgg gcgccagacc cgacggcttc
     2581 gagcaggccc ggcaccgccg cctccaggtc ctcgagatag gaccggccgc ggaatgtcgg
     2641 gacggtgagg aggtgggaca ccgatgccgt cgtgagctgc gcgagcagct cgggcgggcg
     2701 gagcagggtg ctgagtggca ccagcaccgc ccccacccgc aggaccgcgt aagcgatgac
     2761 cgcccagtcg atgccgttgg gggccagcag ggcgacccgg tcacgcttgg ccatcccgtc
     2821 gcccaccagc cgagccgcga ggtcggcgct ggcgtcgtcc aggtcgagat aggtcaccgc
     2881 ggcatcctcg gtgacgaggg ccgggtgatc gggtgccgtg cgtcggcgca gggccaggag
     2941 ggcggggatg gtttcagcgt cgcccg
//
LOCUS       DASTFV010000232         4895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_902665, whole genome shotgun sequence.
ACCESSION   DASTFV010000232 DASTFV010000000
VERSION     DASTFV010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902665"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VFP02_04425"
     CDS             complement(<1..554)
                     /locus_tag="VFP02_04425"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9542299.1"
                     /translation="MTTLPLDPEDPRALGPYTIRARLGEGGMGVVYLADGPDGPVALK
                     VVRRLLAADDTFRTRFRREVLAARRVSGRGVVELLDADTEGDRPYLAMGYVDGPTLLD
                     EVTTSGPVRGERLRALMVALAEAVDTIHRSGVTHRDLKPGNVLLAEDQPVVVDFGVAT
                     ATEATSITVVGTVMGSPGWMAPEQV"
     gene            613..1794
                     /locus_tag="VFP02_04430"
     CDS             613..1794
                     /locus_tag="VFP02_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9542300.1"
                     /translation="MTTAIDRANAPLDFLGLDGLLDDEERLLRDTVRAFVVDRVLPDI
                     AEWFEAGTFPVEMAKEMGALGLLGMHLDGYGCAGTSAVSYGLACQELEAGDSGFRSFV
                     SVQGSLAMFPIHAYGSEEQKQEWLPAMAAGEAIGCFGLTEADSGSDPSSMRTRARRDG
                     DDWVLDGTKMWITNGGLAQVAVIWAVTDDGVRGFVVPTDTPGFAANDIHRKLSLRASV
                     TSELVLTDVRLPASAVLPGVAGMRGPLSCLSEARFGIVCGAVGAARACYEAALAYTKE
                     RHQFDRPLAGFQLVQRKLVEMLVQVNRAGLLALHLGRLKDRGEITPAQVSFGKLDNVR
                     QALDVAREARSLLGANGITLEYPVIRHMNNLESVYTYEGTNEIHTLVLGQAITGVAAF
                     T"
     gene            1802..2941
                     /locus_tag="VFP02_04435"
     CDS             1802..2941
                     /locus_tag="VFP02_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9542301.1"
                     /translation="MRFTEDHDLFRKMVRDVVDREINPHVDEWEAAGRFPAHELFPVL
                     GAAGLLGLEYDPAYGGQGADHSFTVVYGEEIGRIACGGVPMAISVQTDMATPSLHRYG
                     SEELKQQFLVPAVAGTAVAAVAVTEADAGSDVAGLRTRARRDGDEWVIDGSKLYITNG
                     AQADWVCVLARTADEGGSAGISQIIVPTDTPGFSVSRTLAKLGMRSSDTAELVFDGVR
                     VPAGNLIGEEGRGFQQQMEQFQQERMIACYQAVGSMEQAMARTREYLGDRQAFGGPLY
                     GNHYLAYTMAELSAEVDLLKHYNHACAEAFIAGVDTKKMVAVAKLTTGRLLRKVADLC
                     LQYHGGLGYMEEHWTARYLRDQRLWAIGGGTDEIMLRTIAQLDAP"
     gene            3046..4239
                     /locus_tag="VFP02_04440"
     CDS             3046..4239
                     /locus_tag="VFP02_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET9542302.1"
                     /translation="MPSRRPIAVVVVVAAALLGSGTPVLARGDERPPPSTSTTTTSTT
                     LPTGIDVGHGGDQSGVNPLDDPATVEPADPDNVNEVLHISTDEAQARRDAVRAELDQV
                     LAHIARLEADVAAFDQLSADLVVQQRIVARRAVAARKQMVERAVGAYVAGNAPEIEAA
                     YGATEPNEVEERRTIVSTIMEADRDEAERLLARRLDVTARLSRVLEQAEQTQAELRVV
                     RTEMAPLRVRLKAANVTLQVLEAGSQIVITGFVFPVADPHTFSSTFGAPRSGGRTHEG
                     ADIFAPRWTPLVAAERGVIDNVGTGTLGGAKLWVIGESGTQYYYAHLQAYADGVTDGT
                     LVEAGDVVGYTGDTGNAVGTPTHLHFEIHPGGGDAIDPYPLLHAVDIVDGVNLLPRPG
                     GAGRQ"
     gene            4250..>4895
                     /locus_tag="VFP02_04445"
     CDS             4250..>4895
                     /locus_tag="VFP02_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9542303.1"
                     /translation="MAVPDSVIEVEDVEAAAARIAGHVRVTPVLRPGPGAFGSDLDLV
                     LKLELFQHTGSFKPRGAFNRILANEVPPAGVIAASGGNHAAAVAHAASVLALPAEIHV
                     PEAAPAVKLERLARYPVDVRRSGDLYDDAQTVCDLRAAETGALVCHPYDLPEVLAGHG
                     TLARELEDQAPEIDTVLVAAGGGGLIGGVASWYRGRVKVVAVEPETSRCLGAALD"
BASE COUNT          718 a         1743 c         1752 g          682 t
ORIGIN      
        1 acctgctcgg gggccatcca gcccggcgac cccatcacgg tgccgaccac ggtgatcgac
       61 gtggcctcgg tggcggtggc cacgccgaag tcgacgacca ccggctggtc ctcggccagc
      121 aggacgttgc ccggcttcag gtcgcggtgg gtcacgccag accggtggat ggtgtccacc
      181 gcttcggcaa gggcgaccat cagggcccgg agccgttcgc cccggacagg acccgaggtg
      241 gtgacctcgt ccagcaaggt cggaccatcg acgtagccca tggccaggta cgggcggtca
      301 ccttcggtgt cggcgtcgag gagctccacg acgccccggc cgctcacccg gcgggccgcg
      361 aggacctccc gccggaagcg ggtccggaag gtgtcgtcgg cggccaggag ccgtcggacc
      421 accttcagcg cgaccggtcc gtcgggcccg tcggccaggt acaccacgcc catcccgccc
      481 tcacccaacc gggcccggat cgtgtacggg ccgagggcgc gggggtcctc gggatcgagc
      541 gggagggtgg tcatggccgg accattctgg gccgaggtgg ctctcctgcc gcctcagggc
      601 caccatggag gggtgaccac tgccatcgac cgtgccaacg ccccgctcga cttcctgggc
      661 ctcgacggcc tgctggacga cgaggagcgc ctcctgcggg acaccgtgcg ggccttcgtg
      721 gtcgaccgcg tgctgcccga catcgcggaa tggttcgagg ccgggacctt cccggtggag
      781 atggccaagg agatgggtgc gctgggcctc ctgggcatgc acctcgacgg ctacggctgc
      841 gccgggacca gcgccgtcag ctacgggctg gcctgtcagg agctggaggc cggggacagc
      901 gggttccgga gcttcgtgtc ggtccaaggc tcgctggcca tgttccccat ccacgcctac
      961 ggcagcgagg agcagaagca ggagtggctg ccggccatgg cggccggcga ggccatcggt
     1021 tgcttcggcc tgaccgaggc cgactcgggc agcgacccgt cctcgatgcg gacgcgggcc
     1081 cgccgggacg gggacgactg ggtgctcgac ggcacgaaga tgtggatcac caacggcggc
     1141 ctggcccagg tcgcggtgat ctgggcggtg accgacgacg gcgtccgtgg cttcgtcgtc
     1201 cccaccgaca cgcccggctt cgcggccaac gacatccacc gcaagctgtc cctgcgggcc
     1261 tcggtcacct ccgagctggt cctgaccgac gtccggctgc cggcctccgc ggtgctcccg
     1321 ggcgtggccg ggatgcgcgg gccgttgtcg tgtctgtccg aggcccggtt cggcatcgtg
     1381 tgcggcgcgg tcggggcggc ccgggcctgc tacgaggcgg ccctggccta cacaaaggag
     1441 cgtcaccagt tcgaccgccc gttggccggc ttccaactcg tgcaacgcaa gctggtggag
     1501 atgctggtgc aggtgaaccg ggcgggcctc ttggccctgc acctcggccg gctgaaggac
     1561 cggggggaga tcaccccggc tcaggtcagc ttcggcaagc tggacaacgt gcgccaggcc
     1621 ctcgacgtgg cccgcgaggc ccgtagcctg ctcggggcca acggcatcac cctcgagtac
     1681 ccggtcatcc ggcacatgaa caacctcgag tcggtctaca cctacgaagg caccaacgag
     1741 atccacacgt tggtgctcgg ccaggccatc accggcgtgg ccgccttcac ctaggaggag
     1801 catgcgtttc accgaggacc acgacctctt ccgcaagatg gtccgcgacg tcgtggaccg
     1861 tgagatcaac ccgcacgtcg acgagtggga ggcggcgggc cgcttcccgg cccacgagct
     1921 cttcccggtc ctgggggcgg ccggcctgct cggcctcgag tacgaccccg cctacggcgg
     1981 ccagggtgcg gaccacagct tcaccgtggt gtacggcgag gagatcggcc ggatcgcgtg
     2041 tggcggtgtg cccatggcca tttcggtgca gaccgacatg gccaccccct cgctgcaccg
     2101 ctacgggagc gaggagctga agcagcagtt cctggtcccc gcggtcgcgg gcacggcggt
     2161 ggccgcggtc gcggtcaccg aggccgacgc cggctccgac gtagccgggc tgcggacccg
     2221 ggcccggcgc gacggcgacg agtgggtgat cgacggcagc aagctctaca tcaccaacgg
     2281 agcccaggcc gactgggtct gtgtgctggc ccgcactgcc gacgaggggg gttcggccgg
     2341 gatctcccag atcatcgtgc ccacggacac cccgggcttc tcggtcagcc gcaccctcgc
     2401 caagttgggg atgcgctcgt cggacaccgc ggagctggtg ttcgacggcg tgcgggtgcc
     2461 ggccgggaac ctcatcggcg aggagggacg cggcttccag cagcagatgg agcagttcca
     2521 gcaggagcgg atgatcgcct gctaccaggc ggtgggctcc atggagcagg cgatggcccg
     2581 gacacgcgag tacctcggtg accgccaggc cttcgggggt ccgctgtacg gcaaccacta
     2641 cctggcctac acgatggccg agctgtcggc cgaggtggac ctgctcaagc actacaacca
     2701 cgcctgcgcc gaggccttca tcgccggggt cgacaccaag aagatggtgg ccgtggccaa
     2761 gctgacgacc ggacggctgc tgcggaaggt ggccgacctc tgcctccagt accacggcgg
     2821 cctcggctac atggaggagc actggacggc gcggtatctg cgcgaccaac gcctctgggc
     2881 catcggtggc ggcaccgacg agatcatgtt gcgcaccatc gcccaactcg acgccccctg
     2941 acagggcggc acatagaaat gctggaaatc atctgaaaat gacgcgaaga tggccggatt
     3001 ctccgtgccc taaagtctca ccctccgtgg tcgatctcat gggggatgcc ctctcgccgc
     3061 ccgatcgcgg tggtcgtcgt cgtcgccgcc gcgctgctgg ggtccggcac ccccgtcctg
     3121 gcgcggggcg acgagcggcc gccgccgtcg acctcgacca cgacgacctc cacgaccctg
     3181 cccacgggga tcgacgtcgg ccacggcggt gaccagagcg gggtcaaccc cctcgacgac
     3241 cccgccacgg tcgagcccgc cgatcccgac aacgtcaacg aggtgctgca catcagcacc
     3301 gacgaggccc aggcccgcag ggacgccgtg cgggcggagc tggaccaggt gctggcccac
     3361 atcgcccggc tcgaagccga tgtcgcggcg ttcgaccagc tgagcgcgga cctcgtcgtc
     3421 cagcagcgga tcgtcgcccg gcgcgccgtg gccgcccgga agcagatggt cgagcgggcc
     3481 gtcggcgcct acgtcgccgg caacgcgccc gagatcgaag cggcctacgg ggccaccgag
     3541 cccaacgagg tcgaggagcg ccgcaccatc gtgagcacga tcatggaggc cgaccgggac
     3601 gaggccgagc gcctcctggc ccgccgcctc gacgtgaccg cccgtctgag ccgggtgctc
     3661 gagcaggccg agcagaccca ggccgagctg cgggtggtcc ggaccgagat ggctcccctg
     3721 cgggtccgcc tgaaggcggc gaacgtcacc ctccaggtcc tggaggcggg cagtcagatc
     3781 gtcatcaccg gcttcgtctt cccggtggcc gatccccaca cgttctccag cacgttcggt
     3841 gctccccgct cgggcgggcg gacccacgag ggggccgaca tcttcgcgcc gcgctggaca
     3901 ccgttggtgg ccgcggagcg gggtgtcatc gacaacgtcg gcaccggcac gctcggtggc
     3961 gccaagctct gggtcatcgg tgagagcggg acgcagtact actacgccca cctccaggcc
     4021 tacgccgacg gcgtgaccga cggcaccttg gtcgaggcgg gtgatgtcgt cggctacacg
     4081 ggcgacacgg gcaacgccgt cgggacaccg acccacctcc acttcgagat ccaccccggt
     4141 ggtggcgacg ccatcgaccc gtatccgctc ctgcacgcgg tggacatcgt cgacggcgtg
     4201 aacctcctgc cgagaccggg cggcgcgggg cgccaatagc ctccgccggg tggccgtccc
     4261 cgacagcgtc atcgaggtcg aggacgtcga ggcggcggcg gcgcggatcg ccgggcacgt
     4321 gcgggtcacg cccgtcctgc gcccgggccc gggcgccttc gggtcggatc tcgacctggt
     4381 cctgaagctg gagctgttcc agcacacggg ctcgttcaag ccccgggggg cgttcaaccg
     4441 catcctcgcc aacgaggtcc ctcccgctgg ggtgatcgcc gcgtccgggg gcaatcacgc
     4501 cgccgccgtg gcccacgccg cctcggtgct ggcgctgccg gccgagatcc acgtgcccga
     4561 ggcggcgccg gccgtcaagc tcgagcgcct ggcccgctac ccggtcgacg tgcgtcgcag
     4621 cggggacctg tacgacgacg cgcaaactgt gtgtgacctg cgggcggccg agaccggcgc
     4681 cctggtctgt cacccctacg acctccccga ggtgctcgcc ggccacggga cgctggcccg
     4741 cgagctcgag gaccaggcgc cggagatcga cacggtgctc gtcgccgccg gtggcggtgg
     4801 gctgatcggt ggcgtggcct cctggtaccg cgggcgggtc aaggtggtgg ccgtggagcc
     4861 ggagacctct cggtgcctcg gggcggccct cgatg
//
LOCUS       DASTFV010000233         2239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_907864, whole genome shotgun sequence.
ACCESSION   DASTFV010000233 DASTFV010000000
VERSION     DASTFV010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907864"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VFP02_04450"
     CDS             <1..909
                     /locus_tag="VFP02_04450"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HET9542304.1"
                     /translation="PCDIVAALRTFHHAPFAPADLVAAKEGRRVSVCLPARDEAETVG
                     SIVAAVRKELIEAVALVDEILVLDDHSADATATTAADAGARVVPAGDVAPEFGDEPGK
                     GQALWKTVLVSQGDLIVWCDADVRDFDPAFVTGLLGPLLTEPDLAFVKGYYERPETGG
                     QPGGGRVTELLARPALALLHPTLAAMVQPLSGEYAARRSLVEALPFVEGYGVDIGLLL
                     DAEAAVGIRGLAQVDLGERRHRNRSLAELSVQATEVLHTILDRADVADLPESVVLDRP
                     AEGPARVRTGERPAPRTVAGYERRTA"
     gene            944..1723
                     /locus_tag="VFP02_04455"
     CDS             944..1723
                     /locus_tag="VFP02_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9542305.1"
                     /translation="MSTPDLFSLDGRTAVVTGGTSGIGRMIADGLIDAGVRVYVASRK
                     ADACARVAAELSERGTCIGIAADLSTEAGCLALATEIAEREDRIHLLVNNAGATWGAP
                     LEEYPDAAFDKMFAINVKGVFHLTRTLLPQLRAAATEEDPARVVNIGSIDGIRVPITE
                     NYAYSATKAAVHMLTRHLAATLVKDHITVNAIAPGPFDSRMMAFVLDDPAGRQAVSAS
                     VPIGRVGQPSDVAGTVIFLASKAGSYLTGAVIPVDGGVSTR"
     gene            complement(1724..1834)
                     /locus_tag="VFP02_04460"
     CDS             complement(1724..1834)
                     /locus_tag="VFP02_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9542306.1"
                     /translation="MVGSVGLVGARRVLLGSVATTVLHHAPCDVLVVDTA"
     gene            complement(1995..>2239)
                     /locus_tag="VFP02_04465"
     CDS             complement(1995..>2239)
                     /locus_tag="VFP02_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET9542307.1"
                     /translation="TPDNPLEGLALSTTVVLFTAPFNISGQPAISLPLHWTDDGLPVG
                     VQLVAAYGREDLLLRVAAQLEAAAPWADRTPPIFAG"
BASE COUNT          308 a          825 c          810 g          296 t
ORIGIN      
        1 ccgtgcgaca tcgtggctgc gctgcgcacc ttccaccacg cccccttcgc cccggccgat
       61 cttgtcgcgg cgaaggaggg gcgacgggtc tcggtgtgcc tccccgcccg cgacgaggcc
      121 gagacggtgg gctcgatcgt ggccgcggtc cgcaaggagc tgatcgaggc tgtggcgctc
      181 gtcgacgaga tcctggtcct ggacgaccac tccgctgacg ccaccgcgac gacggcggcc
      241 gacgccgggg cgcgcgtcgt tcccgccggc gacgtcgccc ccgagttcgg tgacgagccg
      301 ggcaagggcc aggccctgtg gaagaccgtc ctggtcagcc agggcgacct catcgtctgg
      361 tgtgacgccg acgtccggga cttcgacccg gccttcgtga ccgggctgct cgggccgctg
      421 ctcaccgagc cggacctcgc gttcgtcaag ggctactacg agcgccccga gaccggcggc
      481 cagccgggcg gtggtcgggt caccgagctg ctggcccggc cggccctggc cctgctccat
      541 ccgacgctcg cggccatggt gcagcccctg tccggggagt acgcggcccg ccgatcgctg
      601 gtcgaggccc tgccgttcgt cgagggctac ggcgtcgaca tcggcctgct cctcgacgcc
      661 gaggctgcgg tcgggatccg gggcctggcc caggtcgatc tcggggagcg tcggcaccgc
      721 aaccggagcc tcgccgagct gtcggtgcag gccaccgagg tgctgcacac gatcctcgac
      781 cgggccgatg tcgccgacct cccggaatcg gtcgtgctcg accggccggc agagggcccg
      841 gcccgggtcc ggacgggcga gcgccccgcc ccgcggaccg tcgccgggta cgagcgccgc
      901 accgcctgac cgtcggttcg gcgccgggca ccagaatggg cccatgagca ccccagacct
      961 cttctccctc gacggccgca ccgcggtcgt caccggcggc accagcggga tcgggcgcat
     1021 gatcgccgac gggctgatcg acgccggggt ccgcgtctac gtcgcctcgc gcaaggcgga
     1081 cgcctgcgcc cgcgtggcgg ccgagctgtc cgagcggggg acttgcatcg gcatcgccgc
     1141 tgacctgtcg accgaggcgg gctgcctggc cctcgccacg gagatcgccg agcgcgagga
     1201 ccgcatccac ctcctggtca acaacgccgg ggccacctgg ggggcgccgc tcgaggagta
     1261 ccccgacgcc gccttcgaca agatgttcgc catcaacgtg aagggggtgt tccacctcac
     1321 ccgcacgctc ctcccccagc tacgggccgc ggcgaccgag gaggacccgg cccgggtggt
     1381 gaacatcggc tcgatcgacg gcatccgcgt gcccatcacc gagaactacg cctactcggc
     1441 gaccaaggcc gcggtccaca tgctcacccg ccacctggcg gcgaccctgg tgaaggacca
     1501 catcacggtc aacgccatcg cccccggccc gttcgacagc aggatgatgg ccttcgtgct
     1561 cgacgatccc gccgggcgcc aggccgtgtc cgcctcggtg ccgatcgggc gcgtcggaca
     1621 accgagtgac gtggccggca cggtgatctt cctggcctcg aaggcgggca gctacctgac
     1681 tggcgccgtg atccccgtcg acggcggggt gagcacgagg tagctacgcc gtgtccacca
     1741 cgagcacgtc gcacggggcg tggtggagga ctgtggtggc cacgctgccg aggagcacgc
     1801 gccgggcccc gaccaggccg accgagccga ccacaggagg tcggcgtcgt gcagcccggc
     1861 ggcgtccagg aggacctcgc cggggtcgcc gtcgtcgctc tggagcacga tcccctccaa
     1921 ggtgccggcc aaggtggccc accacctgcc ctgcagcctc ctggtcgtga agacgacctg
     1981 aggcaatcgg tcgctcagcc cgcgaagatc ggcggggtgc ggtcggccca gggggcggcc
     2041 gcttcgagtt gggccgccac ccgaaggagc aggtcctccc gtccgtaggc cgccacgagc
     2101 tggacgccga cgggcaggcc gtcgtcggtc cagtggaggg gcagggagat ggccggctgg
     2161 cccgagatgt tgaacggggc cgtgaagagc accacggtgg tggacagggc caggccctcg
     2221 aggggattgt cgggcgtcc
//
LOCUS       DASTFV010000234         3523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_911591, whole genome shotgun sequence.
ACCESSION   DASTFV010000234 DASTFV010000000
VERSION     DASTFV010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911591"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFP02_04470"
     CDS             <1..681
                     /locus_tag="VFP02_04470"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET9542308.1"
                     /translation="REEVAAHNVIGTLPGASAEWMIIASHHDAPWASAVEDGSGIAMV
                     LAQATHWASVPAEERPHNLLFLLTTGHMVHGAGTRSFIDAHQDLLGDVVLEIHLEHTA
                     SEVRGDGHGGLEPTGEPEVRWWFTTQEPELEASVQSALEAEDLRRSLVLRPDIFGPYP
                     TTDGGFFHGEGVPLVNFLTAPMYLFDSADTIDKIHVDSLEPVGRAVVRIVESLRGRTA
                     ANLRAVSR"
     gene            complement(678..1688)
                     /locus_tag="VFP02_04475"
     CDS             complement(678..1688)
                     /locus_tag="VFP02_04475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET9542309.1"
                     /translation="MKLGMTWGYWGQRPPPDIAGVSQEAERLGFDSIWTAEAWGSDAF
                     TPLAWIAAHTERVRLGTAVVQLAARTPTATAMHALTLDHLSNGRLILGLGVSGPQVVE
                     GWYGRPSNKPLARTREYVEIIRRAIAREEPVEFDGEFFQHPYRGEGSVGLGKALKPIT
                     HPLRREIPIFLGAEGPKNVTQTAAIADGWLPLYYSPYRQEVYADQLAGAKDGFEITAM
                     ATMIVTDDIEAGLAPVKAMLGFYIGGMGAKGQNYHTKLMQRMGYEAEALKVQELFFEG
                     RRDEAIAAVPSAFADEISLVGPIERIRDRVAAWEDSPVTTILVHGGNVEGLRHAAEAV
                     LG"
     gene            complement(1807..2460)
                     /gene="msrA"
                     /locus_tag="VFP02_04480"
     CDS             complement(1807..2460)
                     /gene="msrA"
                     /locus_tag="VFP02_04480"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET9542310.1"
                     /translation="MLFGSKKDQLVSAEAALPGRPEPLWSPPAHFVTGASLTPPFPEG
                     SEQLVVGMGCFWGAERLFWPIPGVLTTAVGYAGGTTPNPTYEEVCSGLTGHTEAVLVV
                     FDPQVVSREAVLKAFWEGHDPTQGMRQGNDRGTQYRSAIYTADDAQQAAAEASRDRFQ
                     AVLNESGFGEITTEIRPAGEFFYAEDYHQQYLAKVPNGYCGLGGTGVSCPIGVASTD"
     gene            complement(2497..>3523)
                     /locus_tag="VFP02_04485"
     CDS             complement(2497..>3523)
                     /locus_tag="VFP02_04485"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HET9542311.1"
                     /translation="PDDPIIEAYRGAFPELFSDLDEMPAEMREHLRYPEDLFRVQTNT
                     WGRYHIDGAQNFYEQAGGWAVAQDPGTTTSATPQTQTTNAQGEVEVAPERRIDPQYLL
                     MRLPGEEQEDFLILRSFVPKAGDTERRELTAFMVAKSDPDNYGEIEVFEMQSADIAGP
                     AIVSSNIQSDSTIAREITLLDQQGSNVLFGNLLLIPVEESILYIRPLYTEAEGQTSVP
                     LLRRVIVAYGDEIVMEPTLCEALIQIFGEAPETQEGACTPGGGSPPDDGSTPPEDVDP
                     TAEVADLLAQADDLFSQAEEGLQDNGDLGEYQDLVQQAQDLIQQALALSDPTATTTTT
                     TVPAGEA"
BASE COUNT          506 a         1224 c         1233 g          560 t
ORIGIN      
        1 cgcgaggagg tggcggccca caacgtcatc ggcacgctcc ccggcgcctc cgccgagtgg
       61 atgatcatcg cctcccatca tgacgccccc tgggcatcag cagtggagga cggcagcggc
      121 atcgccatgg tcctggccca ggccacccac tgggcctcgg tgcccgccga ggagcggccg
      181 cacaacctgc tgttcctgct cacgaccggg cacatggtcc acggcgccgg cacccggagc
      241 ttcatcgacg cccaccaaga cctgctcggc gacgtcgtcc tcgagatcca cctcgaacac
      301 accgctagcg aggtgcgggg tgacggccac ggcggcctcg agccgaccgg cgagcccgag
      361 gtgcggtggt ggttcacgac ccaggagccc gaactggagg cgagcgtcca gtccgcactg
      421 gaggccgagg acctccgtcg ctccctggtc cttcgcccgg acatcttcgg cccgtacccc
      481 acgaccgacg gtggcttctt ccacggcgaa ggggtgccgt tggtcaactt cctgaccgcc
      541 ccgatgtacc tgttcgactc ggccgacacg atcgacaaga tccacgtcga cagcctcgag
      601 cccgtcggcc gggccgtcgt gcgcatcgtc gaatccctcc gcggccgtac cgcggcgaac
      661 ctccgggcgg tcagccgcta gcccagcacg gcctcggcgg cgtggcgtag gccctcgacg
      721 ttgccaccgt gcaccaggat cgtggtcacc gggctgtcct cccaggccgc gacacggtcc
      781 cggatccgtt cgatggggcc gaccaggctg atctcgtcgg cgaaggcgct cggcacggcg
      841 gcgatggcct cgtcccgccg gccctcgaag aacagctcct ggaccttcag ggcctcggcc
      901 tcgtagccca tccgctgcat gagcttcgtg tggtagttct ggcccttggc ccccatgccc
      961 ccgatgtaga agccgagcat ggccttcacc ggggccaagc cggcttcgat gtcgtcggtg
     1021 acgatcatcg tggccatggc cgtgatctcg aagccgtcct tggccccggc cagctgatcg
     1081 gcgtagacct cctggcggta gggcgagtag tagaggggca gccagccatc ggcgatcgcg
     1141 gccgtctggg tgacgttctt cgggccctcg gcgccgagga agatcgggat ctcccggcgc
     1201 agcgggtggg tgatcggctt gagggccttg cccagaccca ccgacccctc accccggtac
     1261 gggtgctgga agaactcgcc gtcgaactcg accggctcct cgcgggcgat ggcccgtcgg
     1321 atgatctcca cgtactcccg ggtccgggcc agcggcttgt tcgacggccg gccgtaccag
     1381 ccctcgacca cctggggccc ggacacaccg agaccgagga tgaggcggcc gttggacagg
     1441 tggtccaggg tcagggcgtg catggcggtc gccgtcggcg tccgggccgc cagctgcacg
     1501 acggcagtac cgaggcgcac ccgctcggtg tgggccgcga tccaggccag gggcgtgaag
     1561 gcgtccgagc cccaggcctc ggccgtccag atggagtcga agccgagccg ctcggcctcc
     1621 tggctcaccc ccgcgatgtc cggcggcggc cgttgccccc agtaccccca cgtcatgccc
     1681 aacttcatgt gaacgctccg ttgcattgct tcgctccgct gcgccggctt cgccgatcag
     1741 gggagtcctc cgagctgctc cgctccgctg cgcagcgtac gacccgctgg acccaggagc
     1801 gaagcgtcag tcggtggagg cgacgccgat ggggcagctg acgccggtgc cgccgagacc
     1861 gcagtagccg ttggggacct tggccaggta ctgctggtgg tagtcctcgg cgtagaagaa
     1921 ctcgccggcc gggcggatct cggtggtgat ctcgccgaag ccggactcgt tcagcacggc
     1981 ctggaagcgg tcccgggacg cctcggccgc ggcctgctgg gcgtcatcgg ccgtgtagat
     2041 ggccgagcgg tactgggtgc cccggtcgtt gccctggcgc atgccctggg tcgggtcgtg
     2101 gccctcccag aacgccttca ggacggcctc ccggctgacc acctgcgggt cgaacacgac
     2161 gaggacggct tcggtgtggc cggtcaggcc agagcacacc tcttcgtagg tggggttcgg
     2221 cgttgtgccg ccggcatagc ccacggctgt ggtgagcacg ccggggatcg gccagaacag
     2281 gcgctcggcg ccccagaagc agcccatccc gacgaccagc tgctcgctgc cctcggggaa
     2341 cgggggcgtg agcgaggcac cggtgacgaa gtgggccggg ggcgaccaga ggggctcggg
     2401 gcggcccggc agggccgcct cggcggagac gagctggtcc ttcttggatc cgaagagcat
     2461 gaacctgaca acgccggaac ggccccggac gttccctcag gcctcgcccg ccgggaccgt
     2521 ggtggtggtc gtggtggccg tggggtcact cagcgccagc gcctgctgga tgaggtcctg
     2581 agcctgctgg acgaggtcct ggtactcgcc caggtcgccg ttgtcctgca ggccttcctc
     2641 ggcctgcgag aacaggtcat cggcctgagc caacaggtcc gccacctccg ccgtgggatc
     2701 gacgtcctcg ggcggcgtcg aaccgtcatc cggaggagag ccgccgccgg gtgtgcacgc
     2761 cccctcctgt gtctccgggg cctcgccgaa gatctggatg agggcctcgc agagcgtggg
     2821 ctccatcacg atctcatcgc cgtaggccac gatcacccgg cgcaggaggg gcacactggt
     2881 ctgaccctcg gcctcggtgt agagcgggcg gatgtagagg atcgactcct cgaccgggat
     2941 cagcagcagg ttgccgaaca gcacgttgga gccctgctgg tccagcaggg tgatctcccg
     3001 cgcgatggtc gaatcactct ggatgttcga gctcacgatg gccgggcccg cgatgtccgc
     3061 ggactgcatc tcgaagacct cgatctcccc gtagttgtcg gggtcgctct tggccaccat
     3121 gaacgccgtg agctcccgcc gttcggtgtc accggccttg ggcacgaacg agcgcaggat
     3181 caggaagtcc tcctgctcct caccagggag gcgcatgagc aggtactgag ggtcgattcg
     3241 ccgttccgga gcaacctcca cctcgccctg ggcgttggtg gtctgggtct gcggcgtggc
     3301 cgacgtggtg gtacccgggt cctgggccac cgcccagccg ccggcctgct cgtagaagtt
     3361 ctgggcgccg tcgatgtggt agcggcccca ggtgttggtc tggacccgga agaggtcctc
     3421 ggggtaccga aggtgctccc gcatctcggc gggcatctcg tcgaggtcgg agaagagctc
     3481 cgggaaggcg ccgcggtacg cctcgatgat ggggtcgtcg gga
//
LOCUS       DASTFV010000235         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_912066, whole genome shotgun sequence.
ACCESSION   DASTFV010000235 DASTFV010000000
VERSION     DASTFV010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912066"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2096)
                     /locus_tag="VFP02_04490"
     CDS             complement(<1..2096)
                     /locus_tag="VFP02_04490"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET9542312.1"
                     /translation="MGMTAPTGHVDLVVEGMTCASCAARIEKRLNGLDGVAATVNFVT
                     ETASIEAPNGVTTDDLVAQVEAVGYGARVPTRVAATTPDEDGDALRPVREGLVFIGLL
                     TLPVVALSMVPALQFDDWQWLALALAAPVVAWGGWPFHVAAWANLRHGAATMDTLISI
                     GTLAAFGWSLYALFLGDAGVTGMRMAVDLGLRRDGGAEQIYLEVATGVTFFILLGRYL
                     EARAKQRSGAALRALLDLGAKQVSILRDGAEARRPVEDLVVDDRFVVRPGETVAADGV
                     VEDGVSAIDASLLTGESVPVEVGPEAALTGGTVNVGGRLVVRATRVGADTTLARIGRL
                     VTDAQSGKAPVQRLADRVAAVFVPIVIGLSLATLAFWLAVGDSPEAAFSAAVAVLIIA
                     CPCALGLATPTALLVGTGRGAQLGILIKGPDVLESTRQVDTIVLDKTGTVTSGVMALI
                     DVVTTPETNRVEALRLVGALEGASEHPVGRAIADGAEAEVGAVPAVVDFHSTPGLGVR
                     GTVDGRSVVVGRADHVLDAGSTVPDRLARAQEEAEAAGRTSVLAGWDGAIRAVLVVAD
                     TVKPTSAAAVRALRELGLHPVLLTGDNDRAARTVADAVGVDEVVAGVLPEGKVAAVRR
                     LQSEGRVVAMVGDGVNDAAALAQADLGLAMGTGTDVAIEAGDITLVRGDLQSAADAIR
                     LSRRTLRTIKGNLFWA"
     gene            complement(2227..>2476)
                     /locus_tag="VFP02_04495"
     CDS             complement(2227..>2476)
                     /locus_tag="VFP02_04495"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9542313.1"
                     /translation="FYVDISVADPGGSYGAWMLSIAGFSNVLNNFGGGIGQIAIHGWS
                     DESVIGQAVSNGCLRMQNGVIERLAPLAPVGPPVTIRA"
BASE COUNT          363 a          901 c          907 g          305 t
ORIGIN      
        1 aaggcccaga agaggttgcc cttgatcgtg cgcagggtgc gccgggacag ccggatggca
       61 tcggccgccg actgcaggtc tcctcgcacc agcgtgatgt cccccgcctc gatggccacg
      121 tcggtgcctg tgcccatggc caggccgaga tcggcctggg ccagtgccgc ggcgtcgttc
      181 acgccgtcgc ccaccatggc caccaccctg ccctcggact ggagccggcg gacggcggcg
      241 accttgccct cgggcaggac accggccacc acctcgtcga cgccgacggc atcggccacc
      301 gtgcgagcgg cgcggtcgtt gtcaccggtg agcaggaccg ggtgcaggcc cagctcgcgg
      361 agggcacgga cggcggcggc cgaggtcggc ttcaccgtgt cggccacgac gagcacggct
      421 cggatcgcgc cgtcccaacc ggcgaggacc gacgtccggc ccgccgcctc cgcctcctcc
      481 tgggcccggg ccaaccggtc cggcaccgtg ctcccggcgt cgagcacgtg gtccgcccgg
      541 ccgaccacga ccgaccgacc gtcgacggtg ccgcggacac cgaggcccgg ggtgctgtgg
      601 aagtcgacga ccgcgggcac cgcgccgacc tccgcctcgg ccccgtcggc gatggcccgg
      661 ccgaccgggt gctcgctggc cccttccagg gcgcccacga ggcgcagggc ctcgacccga
      721 ttggtctccg gcgtggtcac gacgtcgatc agagccatca cgccgctggt cacggtgccg
      781 gtcttgtcca ggacgatcgt gtcgacctgt cgggtcgact ccaggacgtc gggacccttg
      841 atgaggatgc ccagctgggc cccccgaccg gtgccgacca ggagcgcggt cggcgtggcc
      901 aagccgagag cgcacgggca ggcgatgatc aggacggcca cggcggcgct gaacgcggcc
      961 tccggcgaat cgccgaccgc cagccagaag gccagcgtgg ccagggagag gccgatgacg
     1021 atcggcacga agaccgccgc cacccgatcg gccagtcgtt gcacgggcgc cttgccgctc
     1081 tgggcgtcgg tcaccaggcg gccgatccgg gccagggtgg tgtcggcgcc gacccgggtg
     1141 gcgcggacga ccaggcggcc gcccacgttg acggtgccac cggtgagcgc ggcctccggc
     1201 ccgacctcga cggggacgct ctcgccggtg agcagcgagg cgtcgatggc ggagacgccg
     1261 tcctcgacca cgccgtcggc ggccaccgtc tcgccgggac gcacgacgaa gcggtcgtcg
     1321 accacgaggt cctcgaccgg ccgacgcgct tcggcgccgt cgcgcaggat gctgacctgc
     1381 ttggccccga gatcgagcag ggcccggagg gcggcgcccg aacgctgctt ggcccgggcc
     1441 tccaggtagc gccccaggag gatgaagaag gtgacgccgg tcgccacctc gaggtagatc
     1501 tgctccgcgc caccgtcgcg ccggaggccc aggtccaccg ccatgcgcat gccggtcacc
     1561 cccgcgtccc ccaggaacag ggcgtagagc gaccagccga acgcggccag cgtgccgatc
     1621 gagatgaggg tgtccatggt cgccgcccca tggcggaggt tggcccacgc cgccacgtgg
     1681 aacggccacc cgccccaggc cacgaccggc gcggccaagg ccagagccag ccactgccag
     1741 tcgtcgaact ggagcgccgg gaccatcgac agggccacca ccggcagggt cagcaggccg
     1801 atgaagacca gcccctcccg tacgggccgt agggcgtcgc cgtcctcgtc cggcgtggtg
     1861 gcggccactc gggtcggcac gcgggccccg tacccgacgg cctcgacctg ggcgaccagg
     1921 tcgtcggtgg tcaccccgtt cggggcctcg atggaggcgg tctcggtgac gaagttgacg
     1981 gtggcggcca ccccgtcgag gccgttgagg cgcttctcga tgcgggccgc gcaggaggcg
     2041 caggtcatgc cctcgacgac gaggtcgacg tggccggtcg gcgccgtcat acccatgggg
     2101 ggtatcctat cgcggcgttc agcggcgacc cgagcgtgac gctgcgcagt cggagcggag
     2161 cagctcggag aacccagagc ttcggcggcg ccggagttgc tgagcgcagc gaagccactc
     2221 agatgtctat gccctgatgg tcaccggggg gccgacgggc gcgagggggg cgaggcgctc
     2281 gatgacaccg ttctgcatgc gaaggcagcc gttgctgacc gcttggccga tcaccgactc
     2341 gtcggaccag ccgtggatgg cgatctgacc gatgcccccg ccgaagttgt tgaggacgtt
     2401 ggagaagccc gcgatgctga gcatccaggc gccgtaggag ccgcccgggt cggccaccga
     2461 gatgtcgacg tagaac
//
LOCUS       DASTFV010000236         4935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_912463, whole genome shotgun sequence.
ACCESSION   DASTFV010000236 DASTFV010000000
VERSION     DASTFV010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912463"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VFP02_04500"
     CDS             <1..188
                     /locus_tag="VFP02_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9542314.1"
                     /translation="LRESYACGDECANVGRITTTLPDGNRSVVEGVYTYRVDGDGRIV
                     ALRAFWEFDAMTFEVST"
     gene            196..867
                     /locus_tag="VFP02_04505"
     CDS             196..867
                     /locus_tag="VFP02_04505"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9542315.1"
                     /translation="MSFAEFDYGPDIPGETGLRLLGPVAGKRILVLGCRRTDAILSLA
                     RNDAKVIVVDHVPRRVDKARDACEQADVRVEFHRTDLADLAFLRGDSIDATLSVSALA
                     EVEDLNRVFRQVHRVLGANAALALSLPHPAFACLDPDSGEPPRLARRYADAEPLPGRA
                     DGAEAEYAHTIGGLFTSLTRAKFRVDTFLEPEPVAGGARSTHWAAAMAWMPATLVLRA
                     RKEGS"
     gene            872..1384
                     /locus_tag="VFP02_04510"
     CDS             872..1384
                     /locus_tag="VFP02_04510"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9542316.1"
                     /translation="MDRCAACGFDYYGFPRPEIGDRLRALGDEHAERLTETPVAFLRQ
                     HPVDGWSALEYGCHVRDVLLTQRGRIELTQREDAPTFVPMGRDELAVNERYNDQAPDT
                     VALRLTQAAGALATLLAALDDTGWARTATYNYPEPALRDVDWIARHTVHELHHHLTDV
                     HRVLKAVLED"
     gene            complement(1765..3159)
                     /locus_tag="VFP02_04515"
     CDS             complement(1765..3159)
                     /locus_tag="VFP02_04515"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET9542317.1"
                     /translation="MIPERLQRTLEPVLDVVAPLAETFAEADRRLYLVGGVVRDLLVG
                     RDLADRDIDLTTDARPDQIKALIADHVDAVWTLGERFGTIGAKIGEWTVETTTHRADV
                     YVPKSRKPDVQFSTEVESDLSRRDFTVNAMALALPGLDLIDPFDGAADLAASRLRTPL
                     EPQVSLSDDPLRMLRAARFLAGYRLAPSAALVQAVREMGDRLAIVSAERIRDELDKLM
                     VVDDPSDGLWFLVETGLADEFLPELLEMRLEQDPIHQHKDVLAHTIAVVANTEPDRLV
                     RLAALFHDVGKPKTRSFGANGVMFHHHEVVGARMTKDRMTALRYPNEDVAIVTQLVRL
                     HLRFHGYGTGTGWSDAAVRRFVRDAGDQLDRLIELTRCDCTTRNQRKADMLARRMDDL
                     VARIAELEAEEELKAIRPDLDGRQVMDHLGLTPGRDVGEALEMLLEVRLDEGPLGEDE
                     AYRRLDAWWAARST"
     gene            complement(3156..4181)
                     /locus_tag="VFP02_04520"
     CDS             complement(3156..4181)
                     /locus_tag="VFP02_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HET9542318.1"
                     /translation="MAAVLLATHPRFGDHDTGPGHPERPARLAAVLDGLTTAGLDDAL
                     VRVDPRPATRAELEAVHPAAHLDRLEALCRSGGGHVDADTRVSEASWAVALLAAGAGI
                     DSAERLERGEADAAFLAVRPPGHHATVDRAMGFCLLNNVAVTATHLADRGERVLVVDI
                     DAHHGNGTQEVFWSDPRVLYVSFHQHPLYPGTGAVQDVGTGGAAGTTVNFPLPAGATG
                     DVYRAGIEEVLAPRVAAFAPTWLLISAGFDAHRRDPITDLGLTAGDYADVTVDLLAFA
                     PAGRRLVFLEGGYDLTALGECAAATVGALLGEAVQSEAATTAGPGREVLAVVAAAQAR
                     ADEAAQA"
     gene            4324..>4935
                     /locus_tag="VFP02_04525"
     CDS             4324..>4935
                     /locus_tag="VFP02_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9542319.1"
                     /translation="MTTAPPHRALRVRPWSVDPATAHLVPYATRPITADALRRVLEDL
                     TEAGYERVRTAALSPDEQGPFLDVGFEVDEHLHLLAHDLTGLSPPRRPSRLRRARRRD
                     KPAVLAVDQAAFSPPWRLDAAGLREALVATPSIHFRVALVDGAVVGYAVAGRAASRGY
                     IQRVAVHPHHDGHGLGRELVTDALHWLHRRGARRAVVNTQEGNV"
BASE COUNT          719 a         1805 c         1752 g          659 t
ORIGIN      
        1 ccctccgcga gtcctacgcc tgcggcgacg aatgcgccaa cgtcggccgc atcaccacca
       61 cgctgcccga cggcaaccgc tcggtcgtcg aaggcgtcta cacctaccgg gtcgacggcg
      121 acggtcgcat cgtcgccctg cgagccttct gggagttcga cgccatgacc ttcgaggtat
      181 ccacatgaga caacaatgag cttcgccgag ttcgactacg gaccggacat ccccggcgag
      241 accgggttgc gcctcctggg ccccgtggcg ggcaagcgca tcctggtcct gggctgccgt
      301 cggaccgacg ccatcctgtc cctggcccgc aacgacgcca aggtcatcgt cgtggaccac
      361 gtaccccgcc gggtcgacaa ggcccgcgac gcctgcgagc aggcggacgt ccgcgtggag
      421 ttccaccgca ccgacctggc cgacctggcc ttcctgcgcg gcgactccat cgacgccacc
      481 ctgtcggtct cggccctggc cgaggtcgag gacctgaacc gcgtgttccg ccaggtccac
      541 cgggtcctgg gcgccaatgc cgccctcgcc ctctcgctcc cccatccggc cttcgcctgc
      601 ctcgaccccg acagcggcga gccaccgcga ctggcccggc ggtacgccga cgccgagccc
      661 ctccccggcc gggctgacgg cgccgaagcc gagtacgccc acaccatcgg tggcctcttc
      721 accagcctca cccgggccaa gttccgggtc gacacgttcc tcgagcccga gccggttgcc
      781 ggcggcgccc gcagcacgca ctgggccgcg gcgatggcct ggatgccggc caccctggtc
      841 ctccgggccc ggaaagaggg ctcgtaggtc ggtggatcga tgtgctgcgt gtgggttcga
      901 ctactacggc ttcccgcggc ccgagatcgg tgaccgcctc cgcgccctgg gcgacgagca
      961 cgccgagcgc ctgaccgaga cgccggtcgc gttcctccgc cagcacccgg tggacgggtg
     1021 gtcggccctc gagtacggct gccacgtccg cgacgtgctc ctcacccagc ggggccgcat
     1081 cgagctgacc caacgcgagg acgcacccac cttcgtgccg atgggccgcg acgagctggc
     1141 ggtgaacgag cgctacaacg accaggcccc cgacaccgtg gcgctccggc tcacacaggc
     1201 cgccggtgcc ctggccaccc tgctggccgc tctcgacgac accggctggg cccgcaccgc
     1261 cacctacaac taccccgagc cggcgctacg ggacgtcgac tggatcgccc gccacacggt
     1321 ccacgagctc caccaccacc tcacggatgt ccaccgcgtc ctgaaggcgg tgctcgaaga
     1381 ctgagcaccg gcccgcagcc agcagtgagg gttcggggtc gaagcggagc accactgcgg
     1441 acctgcagca tcggcggcga gtgaccaccg gcccgcagcc aggagtaagg gtgcgcagtc
     1501 gaagcggagc accactgcgg acctgcagca tcggcggcgc cggagttccc gaacgaagtg
     1561 aaggaactca aaggaaatga gtcagatggc tcatgcagcg gtcaacgctc gatggcatag
     1621 tgacctcttg acgcaccgca gctcgcgagt gatcgaccag gcgtagtcgg tacttgaggg
     1681 acgggctgcg gtgcgaagat cgacggtggt gatgcccgtt gaacagggtc ccgagagggg
     1741 ccctgttctg gttttcggcc gacctcaggt cgagcgggcg gcccaccagg cgtcgaggcg
     1801 gcggtaggcc tcgtcctcgc cgagcgggcc ctcgtcgaga cggacctcca agagcatctc
     1861 cagggcctcg ccgacgtcgc ggccgggtgt caggccgagg tggtccatga cctggcggcc
     1921 gtcgagatcg gggcggatgg ccttgagctc ctcctcggcc tccagctcgg cgatgcgggc
     1981 gacgaggtcg tccatgcgcc gggccagcat gtcggccttc cgctggttgc gggtcgtgca
     2041 gtcacaacgg gtcagctcga tgaggcggtc gagctggtcg ccggcgtccc ggacgaaacg
     2101 gcgcaccgcg gcatcgctcc agccggtgcc cgtgccgtag ccgtggaagc gcagatgcag
     2161 gcgcaccagc tgggtcacga tggccacgtc ctcgttgggg tagcgcagcg ccgtcatccg
     2221 gtccttggtc atccgggctc cgaccacctc gtggtggtgg aacatgaccc cgttggcccc
     2281 gaacgagcgg gtcttgggct tgcccacgtc gtggaacagg gcggccagcc ggaccaggcg
     2341 gtccggttcg gtgttggcca ccacggcgat ggtgtgagcc aggacgtcct tgtgctgatg
     2401 gatggggtcc tgctccagcc gcatctcgag cagctcgggc aggaactcgt cagccagtcc
     2461 ggtctcgacc aggaaccaca ggccgtcgga ggggtcgtcg acgaccatga gcttgtccag
     2521 ctcgtcccgg atgcgctcgg ccgacacgat ggccagccga tcccccatct cgcgcacggc
     2581 ctgcaccagc gcggcggacg gggccagccg gtagccggcc aggaagcgag ccgcccgcag
     2641 catgcgcagg gggtcgtcgc tcaaggacac ctgaggctcg agcggggtgc gcagccggga
     2701 ggcggcaagg tcggcggcgc cgtcgaacgg gtcgatgagg tccaacccgg gcagggccag
     2761 ggccatggcg ttcacggtga aatcccgacg ggagaggtcg gactcgacct ccgtggagaa
     2821 ctgcacatcg ggcttgcggg acttagggac gtagacgtcg gcccggtggg tggtcgtctc
     2881 gaccgtccac tcgccgatct tggccccgat ggtcccgaag cgctcgccca gcgtccagac
     2941 ggcgtcgacg tggtcagcga tcagcgcctt gatctggtcg ggccgggcat cggtggtcag
     3001 gtcgatgtcc cggtcggcca ggtcccggcc gaccaggagg tcgcggacca cgccacccac
     3061 caggtagagg cgacggtcgg cctcggcgaa cgtctcggcc aggggggcaa ccacgtcgag
     3121 cacgggctcg agagtgcgct ggagccgttc ggggatcacg cctgtgcagc ctcgtcggcc
     3181 cgggcctggg cggccgcgac caccgcaagg acctcccggc cggggccggc cgtcgtcgcg
     3241 gcttcgctct ggacggcctc gcccagcagg gcgccgaccg tggccgccgc gcactcaccg
     3301 agagcggtca ggtcatagcc cccctccagg aacaccaggc gacggcccgc gggggcgaag
     3361 gccagcaggt cgacggtcac gtccgcatag tcgcctgccg tgaggccgag gtcggtgatg
     3421 ggatcccgac gatgggcgtc gaagccggcg gagatcagca gccaggtcgg ggcgaaggcc
     3481 gcgacccgcg gggccagcac ctcctcgatg ccggctcgat agacgtcccc cgtggccccc
     3541 gccgggaggg ggaagttgac cgtcgtgccc gcggccccgc cggtccccac gtcctgcacg
     3601 gcgccggtgc ccggatagag ggggtgctgg tggaaggaga cgtagaggac ccgggggtcg
     3661 gaccagaaca cctcctgggt gccgttgccg tgatgggcat cgatgtcgac gaccagcacc
     3721 cgctcgccgc ggtcggcgag gtgggtggcg gtcacggcca cgttgttcag caggcagaag
     3781 cccatcgccc ggtcgacggt ggcgtggtgg ccgggcgggc gcacagcgag gaaggcggcg
     3841 tcggcctcgc cccgctcgag gcgctcggcg ctgtcgatcc cggccccggc ggccaggagg
     3901 gccaccgccc aggaggcctc gctgactcgg gtgtcggcgt ccacgtgacc acccccggag
     3961 cgacagagcg cctccaaacg gtccaggtgg gcggcgggat ggacggcctc cagctcggct
     4021 cgggtggcgg ggcgggggtc gacccgcacc agcgcgtcgt cgaggcccgc cgtggtcagg
     4081 ccgtcgagga ccgcggcgag gcgagccggt cgctcggggt ggcccggccc ggtgtcgtgg
     4141 tcgccgaagc gcggatgggt ggcgaggagc acggcggcca caccggtgac gctagcggcg
     4201 cgcctcacac cccctcccga tcgttctccg tcgtgcggtt cgcgaccagc caccgacgac
     4261 tggacacgga acggccaagg gcggccccga cggggaccgg gcggtcccgt atcctcggcc
     4321 gcgatgacca ccgcaccgcc gcaccgcgcg ctgcgcgtgc ggccctggtc ggtcgacccc
     4381 gccaccgccc acctcgtgcc ctacgccacc cggcccatca ccgccgacgc cctgcgtcgg
     4441 gtcctggagg atctgaccga ggccggctac gagcgcgtgc gcaccgccgc cctcagcccc
     4501 gacgaacagg gacccttcct cgatgtcggg ttcgaggtcg acgagcacct ccacctcctg
     4561 gcccatgacc tgaccgggct gagtccaccc cgccgaccca gccggctgcg acgcgctcgc
     4621 cggcgggaca agccggccgt cctggcggtc gaccaggcgg ccttctcacc cccttggcgc
     4681 ctcgacgccg ccgggctccg cgaggccctg gtcgccaccc cctcgatcca cttccgggtg
     4741 gcgctggtcg atggtgccgt tgtcggctac gccgtggccg ggagggccgc gagccggggc
     4801 tacatccagc gggtcgccgt acacccccac cacgacggcc acggcctggg tcgggaactg
     4861 gtcaccgacg ccctccactg gctgcaccgg cggggggccc gccgggccgt ggtcaacacc
     4921 caggagggca acgtc
//
LOCUS       DASTFV010000237         2632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_916352, whole genome shotgun sequence.
ACCESSION   DASTFV010000237 DASTFV010000000
VERSION     DASTFV010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916352"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VFP02_04530"
     CDS             complement(<1..1193)
                     /locus_tag="VFP02_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9542320.1"
                     /translation="MTAHFGPGEDPAGYDPLARRSLPAAWAERWAAAPEAQVLYEAGR
                     GWVTAGELETATRRVAGRFAGAGLAPGDRVLFSTAPSIALVVAHVAAYRAGLVVVPAN
                     TAYTEREVVHVVRDAGIRAAVVDDAERGRWVRDAGEDDVVVVGPEVDLPDAPAPVLDA
                     VGSDDLAHLGYTSGTTGAPKGAALSHGNMLANSEALERAWRWTADDRLVLALPLFHAH
                     GLCNGLHGTLLAGASAVLLPGFTVDGVLDAAAEHDATLFFGVPTMYHRLAASGRAAEL
                     GRLRLCVSGSAALPAELHRSLQEGAGVTVLERYGMTETNMLVSHPYDGERRAGTVGLP
                     LPGVDVRVAGSAEIEVRGPNVFAGYWLRPEATAESFTHDGWFRTGDLGSWDDDGYLTI
                     LGRAK"
     gene            complement(1190..>2632)
                     /locus_tag="VFP02_04535"
     CDS             complement(1190..>2632)
                     /locus_tag="VFP02_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9542321.1"
                     /translation="WFRNYTTHNAGTVQAVPSLLSGQLPTPGRAPLVTDWPTNLFTLL
                     GGSYDMAVQETVTQLCPPDVCGNDSRTVTQRTPVDGEGLEGAVGDATDVVRQLVSLNA
                     EPEVAIDEFTEETVSVPAPEEMGVEDRNQVTNQPTRFTDFLDGMVRGEDPTLHFVHLI
                     LPHGPWRFYPDGTEYVSPDGDPEGEIAGTWTDAWPAELTRFRLELQAQYTDALVGQTI
                     DRMQESGLWDDALFVVVADHGGAFVVGEPGRALGENNAFEVMWTPLFVRDPDLAHGIN
                     ETDMEATDLLPTMADLLDVDLPYDVDGASAVSAPDTSGTKRYQRLQNAFQVEPDALLE
                     IDTAANYARLLDDHWPVVAVDDPVAGFYRRYPLGDLYGQAVTDLPVGEPAGTATLDQR
                     DALEVGGDGPLPAYLGGEIDVDGAGDDTWVVVAVDGVIQGFSPLFPMVDTESAYSILL
                     SQEAASGAGHQVDLYVTDSPDQPLRPLTLP"
BASE COUNT          362 a          932 c          976 g          362 t
ORIGIN      
        1 tccttggccc gcccgaggat ggtcaggtac ccgtcgtcgt cccacgaccc gaggtcgccc
       61 gtgcggaacc agccgtcgtg ggtgaacgat tcggcggttg cctccggccg cagccagtag
      121 ccggcgaaca cgttgggacc gcgcacttcg atctcggcgg agccggccac ccggacgtcg
      181 acgccgggta ggggcaggcc gacggtgccg gcccggcgct cgccgtcgta ggggtgggac
      241 acgagcatgt tggtctcggt catgccgtag cgctcgagca cggtcacccc agccccttcc
      301 tgcagcgatc ggtggagctc agcggggagc gcagccgatc ccgacacgca gaggcgcagg
      361 cgaccgagct cggcggcccg gcccgaggcc gccagtcggt ggtacatggt cggcacgccg
      421 aagaagaggg tggcgtcgtg ctcggcggcc gcgtcgagca cgccgtcgac ggtgaagccc
      481 ggcagcagga cggccgacgc ccccgccagc agcgtcccgt ggaggccgtt gcagaggccg
      541 tgggcgtgga agagcgggag agcgaggacg agccggtcgt ccgcggtcca acgccaggcc
      601 cgctccagcg cctcactgtt ggccagcatg ttcccgtgac tcagggcggc ccccttcggg
      661 gcgccggtgg tccccgacgt gtacccgaga tgggccaggt cgtccgagcc gaccgcgtcc
      721 agcaccggcg ccggggcgtc ggggaggtcg acctcggggc cgacgacgac gacgtcgtcc
      781 tcgccggcgt cgcgcaccca ccggccccgc tcggcatcgt cgaccactgc cgcccggatg
      841 ccggcgtcgc gcacgacgtg gaccacctcc cgctcggtgt atgcggtgtt ggccggcacc
      901 acgacgagcc cggcccggta ggcggccacg tgggccacga ccagggcgat ggacggcgcc
      961 gtgctgaaca ggacccggtc accaggcgcc aacccggccc cggcgaagcg cccggccacc
     1021 cgacgggtcg ccgtctccag ctccccggcg gtcacccaac cccggcccgc ctcgtacagg
     1081 acttgggcct ccggcgcggc cgcccatcgc tcggcccagg ccgcgggcag gctccgccgg
     1141 gccagcgggt cgtaccccgc cggatcctcg cctggtccga agtgcgcggt cacgggaggg
     1201 tcagggggcg caacggctgg tcggggctgt cggtgacgta gaggtcgacc tgatggccgg
     1261 cgccggaggc ggcctcctgg ctcagcagga tgctgtaggc cgactcggtg tcgaccatcg
     1321 ggaacagcgg ggagaagcct tggatgacgc cgtcgacggc gacgaccacc caggtgtcgt
     1381 ccccggcgcc gtcgacgtcg atctcaccgc cgaggtaggc cggtaggggc ccgtcgccac
     1441 cgacctccaa ggcgtcacgt tggtcgagcg tcgccgtgcc cgccggctcg ccgacgggca
     1501 ggtcggtgac cgcctgcccg tagaggtcgc ccagcgggta gcggcggtag aagccggcca
     1561 cggggtcgtc gaccgccacg accggccagt ggtcgtccag caaccgggcg tagttcgcgg
     1621 cggtgtcgat ctcgagcagg gcgtcgggct cgacctggaa ggcgttctgc agccgttggt
     1681 accgcttggt ccccgacgtg tccggcgccg acacggccga cgccccatcc acgtcgtagg
     1741 gcaggtcgac gtcgagcagg tcggccatcg tgggcagcag gtcggtggcc tccatgtccg
     1801 tctcgttgat gccgtgggcc aggtcggggt cccggacgaa cagcggggtc cacatgacct
     1861 cgaaggcgtt gttctcaccg agggcccgac cgggctcacc cacgacgaag gccccgccgt
     1921 ggtcggccac gacgacgaac agagcgtcgt cccagaggcc gctctcctgc atccggtcga
     1981 tggtctgtcc gaccagggcg tcggtgtact gggcctggag ctccaggcgg aagcgcgtca
     2041 actcggcggg ccaggcgtcg gtccaggtcc cggcgatctc cccttcgggg tcaccgtccg
     2101 gcgacacgta ttcggtgccg tccgggtaga accgccacgg gccgtggggc aggatcaggt
     2161 ggacgaagtg caacgtcgga tcctcacccc ggaccatgcc gtcgaggaag tcggtgaagc
     2221 gggtgggctg gttggtgacc tgattgcggt cctcgacccc catctcctcg ggggccggca
     2281 ccgacacggt ctcctcggtg aactcgtcga tcgccacttc gggctcggcg ttgagcgaga
     2341 ccagctggcg cacgacgtcg gtggcgtcgc cgaccgcccc ctcgaggccc tcaccgtcga
     2401 ccggggtccg ttgcgtcacc gtccgggagt cgttgccaca cacgtccggg gggcagagct
     2461 gggtgacggt ctcctgcacg gccatgtcgt agctgccgcc cagcagggtg aacaggttgg
     2521 tcggccagtc ggtgaccagc ggggcccgcc ccggggtggg cagctgcccg gacagtaggg
     2581 agggcaccgc ctgcaccgtg cccgcgttgt gggtcgtgta gttgcggaac ca
//
LOCUS       DASTFV010000238         3262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_921873, whole genome shotgun sequence.
ACCESSION   DASTFV010000238 DASTFV010000000
VERSION     DASTFV010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921873"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2105
                     /locus_tag="VFP02_04540"
     CDS             <1..2105
                     /locus_tag="VFP02_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HET9542322.1"
                     /translation="VFGNVPDALKVTFYTVIPALLVYGAVLFSFRVRNWQRGAPDDRS
                     TNAGNIKRRLGDFRAGVYMQTLLRDPVAGVMHSLIYVGFLVLTAVTAVLEIDHQLPDD
                     AKFLHGTTYQAYAFVGDAAGLVFLAGIAMAVVRRYVVRPYRIRIKSKPEHALILGLFA
                     VLGLTGFAAEMFRIALNGRPAYEEWSFVGYPLSSLVDGMDSLAGWHQAVWVLHVLCFM
                     AFLAILPVTMLRHMFTSPMNMYLRDRERPKGAMKPLPNLMETELESFGASTVSEMTWK
                     QLLDTDACTMCGRCTSVCPAHATGKPLDPREIVLKVGEVMAASGSPVVSPPIGVDAEI
                     TVASDSLFERITPEELWACTSCKACDEICPVNIEILDKILDMRRYLSLMESNFPTELG
                     NAYRSMENSSNPWGMSQGERADWAKGLEGTDAEIPVIDPGDAFDHEYLYWVGCAGSFD
                     DKNRKVSQAMAKLMARAGLDFAILGPSELCTGDPARRSGNEYLFQMLATQNVEALNGM
                     GVRKIITQCPHCFNTLANEYPQLGGHYDVVHHSQLLETLIETGKLDLSEARLEERLVY
                     HDSCYLGRHNDVYLAPRKVIGSLGGVEVVEAPRNGTKGMCCGAGGARMWMEEHTGKQV
                     NVERSQELLATGASRIATACPFCYIMIDDGVKGEGVDEDQVKVADIAMHVLEAVERGE
                     ATANQPIGAGTGAEALQD"
     gene            2083..2544
                     /locus_tag="VFP02_04545"
     CDS             2083..2544
                     /locus_tag="VFP02_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542323.1"
                     /translation="MLKRSRTDKQRREWEPHGEGVLLVCDDPDAGELLNRLMAADGHD
                     VHRVETAEGAVRHLLATPRRAAVLSLSGPTANRQLLEKIRTHPNPPVSEVAIVLLADD
                     IGAGREAWENGVDGFLARPFHADQLATELSGALDRPLADRQAHRDAQLDAD"
     gene            complement(2611..3174)
                     /locus_tag="VFP02_04550"
     CDS             complement(2611..3174)
                     /locus_tag="VFP02_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9542324.1"
                     /translation="MTADTPDRAAAVRSALRRLVADHGLHGASMAAVAKEAGVATGTA
                     YVYYTSKDELLLAAYLELKVDLGHAAVDGLDRSAPAEARFTSMWRAVHGFLAADPTRA
                     RFLTQVESSPLAAAAHAAALASGVDPVLDEAAAADLADRLRPLPPEVLWDLGFGPAVR
                     LVASGRALDDAGVASLADACWQATTEA"
BASE COUNT          516 a         1188 c         1105 g          453 t
ORIGIN      
        1 aggtgttcgg gaacgtcccc gacgccctga aggtcacctt ctacacggtc atcccggcgc
       61 tgctggtcta cggcgccgtg ctgttctcct tccgggtgcg caactggcag cggggtgccc
      121 ccgacgaccg gtcgacgaac gccggcaaca tcaaacgccg gctgggcgac ttccgggccg
      181 gggtgtacat gcagaccctg ctgcgggacc ccgtcgccgg tgtcatgcac tcgttgatct
      241 acgtcgggtt cctggtgctg accgcggtca ccgccgtgct ggagatcgac caccagctcc
      301 ccgacgacgc caagttcctc cacggcacga cctaccaggc gtacgccttc gtgggcgacg
      361 ccgccgggct ggtattcctg gcgggcatcg caatggcggt cgtccgccgc tacgtggtcc
      421 ggccctaccg gatccgcatc aagtccaagc ccgagcacgc cctgatcctc ggcctgttcg
      481 ccgtgctcgg cctgaccggc ttcgccgccg agatgttccg catcgccctc aacggccggc
      541 ccgcctacga ggagtggtcc ttcgtgggct acccgctgtc gtccctggtc gacggcatgg
      601 actccctggc cgggtggcac caggcggtct gggtgctcca cgtcctgtgc ttcatggcct
      661 tcctggccat cctcccggtg accatgctgc gccacatgtt cacctcgccg atgaacatgt
      721 acctgcggga ccgtgagcgg cccaaggggg ccatgaagcc cctgcccaac ctcatggaga
      781 ccgagctcga gtccttcggc gcctcgacgg tcagcgagat gacctggaag cagctgctcg
      841 acaccgacgc ctgcaccatg tgcggccgct gcacctcggt ctgccccgcc cacgccaccg
      901 gcaagccgct ggacccccgc gagatcgtgc tcaaggtcgg cgaggtcatg gccgccagcg
      961 gctcacccgt cgtgtcgccc cccatcgggg tcgacgccga gatcaccgtc gcgtccgaca
     1021 gcctcttcga gcgcatcacc cccgaggagc tctgggcctg cacctcgtgc aaggcctgtg
     1081 acgagatctg cccggtgaac atcgagatcc tggacaagat cctcgacatg cggcgctacc
     1141 tgtcgctcat ggagtccaac ttcccgaccg agctcggcaa cgcctaccgg tcgatggaga
     1201 acagctccaa cccgtggggc atgtcccagg gcgagcgggc cgactgggcc aagggcctcg
     1261 agggcaccga cgccgagatc ccggtcatcg accccggcga cgccttcgac cacgagtacc
     1321 tctactgggt cggctgcgcc ggcagcttcg acgacaagaa ccgcaaggtc agccaggcca
     1381 tggccaagct gatggcccgg gccggcctcg acttcgccat cctcgggccc tccgagctgt
     1441 gcacgggcga cccggcccgc cgttcgggca acgagtacct gttccagatg ctggccaccc
     1501 agaacgtcga ggccctcaac gggatggggg tgcgcaagat catcacccag tgcccccact
     1561 gcttcaacac gctggccaac gagtaccccc agctcggcgg gcactacgac gtcgtgcacc
     1621 acagccagct gctggagacc ctgatcgaga ccgggaagct ggacctgagc gaggcccgcc
     1681 tcgaggagcg gctcgtctac cacgactcct gctacctggg ccgccacaac gacgtgtacc
     1741 tggcgcctcg gaaggtcatc ggcagcctcg gtggcgtcga ggtggtcgag gcgcctcgca
     1801 acggcacgaa gggcatgtgc tgcggcgccg gcggggcccg catgtggatg gaggagcaca
     1861 ccggcaagca ggtgaacgtg gaacgctccc aggagctgtt ggccacgggc gccagccgca
     1921 tcgccaccgc ctgccccttc tgctacatca tgatcgatga cggcgtgaag ggtgaaggcg
     1981 tcgacgagga ccaggtcaag gtggccgaca tcgccatgca cgtgctcgag gccgtcgagc
     2041 gaggcgaagc caccgccaac caaccgatcg gagccgggac gggtgctgaa gcgctccagg
     2101 actgacaagc agcgtcgcga gtgggagccc cacggtgagg gggtcctgct ggtctgtgac
     2161 gacccggacg ccggggaact gctcaaccgc ctgatggcgg cggacggcca cgacgtccat
     2221 cgggtcgaga ccgcggaagg cgccgtgcgg cacctcctgg ccacgccccg tcgggccgcg
     2281 gtcctctccc tgtcggggcc gacggccaac cgccagctgc tggagaagat ccggacccat
     2341 ccgaacccgc ctgtgtccga ggtggccatc gtgctcctgg ccgacgacat cggggcgggt
     2401 cgcgaggcct gggagaacgg ggtcgacggc ttcctggccc ggcccttcca cgccgatcag
     2461 ttggccaccg agctcagcgg cgccctcgac cgcccgctgg ccgaccgcca agcccaccgc
     2521 gacgcccagc tcgacgcgga ctgacgcgtt ctgatccacc ccgcgaggag ccggcgacgc
     2581 aggagcgtcc ccagaacggt gaggcgtgcc ttacgcctcg gtggtggcct gccagcaagc
     2641 atcggcgagc gaggcgaccc cggcgtcgtc cagcgctcga ccggacgcca ccaggcgaac
     2701 cgccgggccg aagccgaggt cccagaggac ctcgggcgga agcgggcgca agcggtcagc
     2761 caggtccgcg gcggcggcct cgtccagcac cgggtcgacc ccggaggcca gcgcggcggc
     2821 gtgggccgcg gccgccaggg gcgacgactc gacctgggtg aggaagcgtg cccgcgtcgg
     2881 gtcggcggcg aggaagccgt ggacggcccg ccacatcgag gtgaaccggg cctcggccgg
     2941 tgccgaccgg tccagcccgt cgaccgcggc gtggccgagg tcgaccttca gctcgaggta
     3001 ggcggcgagg aggagctcgt ccttcgacgt gtagtagacg tacgccgtcc cggtggccac
     3061 cccagcctcc ttggccacgg ccgccatcga tgccccgtgc aggccgtggt cggcgaccag
     3121 gcgacgcagg gccgaccgga ccgcggcagc ccggtccggg gtgtcggcgg tcaacggagc
     3181 gccgacaggg cggcggcgac gtcgagggtc tcgcccgtct cccgctcgga gacctcccac
     3241 agcacgtcga ccgaacggga ca
//
LOCUS       DASTFV010000239         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_923353, whole genome shotgun sequence.
ACCESSION   DASTFV010000239 DASTFV010000000
VERSION     DASTFV010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923353"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VFP02_04555"
     CDS             complement(<1..863)
                     /locus_tag="VFP02_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9542325.1"
                     /translation="MTHQLADLWEGVVDELGDEREAVVVRSETLGDRRLTHAELEDRA
                     NRLAHHLQGCGVVAGSTIGCHVHNGTEYIEAMLAAYKLRAIPINLNHRYVAEELRYHF
                     EDASPVVVITEADLVDRVNEAGPPPHILVRGDDYEAALAAASPERIAVPDRSNDDLYL
                     LYTGGTTGMPKGVMWRHEDLFFGVIGGAGVPRRGIPRLDDPTDIGAWARKGTGVDRRL
                     PLAPLMHGLAQWTALTTLYTGGTLFLDGDRSFDPVRALTIVAEERIQLIQLVGDVLAL
                     RLAEELHRHGDR"
     gene            complement(871..>2141)
                     /locus_tag="VFP02_04560"
     CDS             complement(871..>2141)
                     /locus_tag="VFP02_04560"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET9542326.1"
                     /translation="GATPPPFWNLELAVGDEFPVPEPSLTALPLAGVRVIDATAWWAG
                     PEAGRLLALLGADVLHLEATRQPDGMRMAGGLFVDQPRWWERSAIALSANTGKRGITL
                     DLTTDDGRDLLDRLLADADVLLENFSPRVAERFALTPERLHALNPRLVLCRMPAFGLD
                     GPWRDRVGFAQTMEQVTGMAWITGHVDDQPRIPRGPCDPLAGAHAAFAVLTALRERER
                     TGTGAFIESTMVEAALNIAAEQVIEWTANGVELQRRGNRAPGFSPQGLYPCAGEEQWL
                     AVSVATDEQWAGLVAVTGLTADRDDEDEVDAELAAWAADRDLQATVDDLIAAGVPAGA
                     ARDPRTIATHPQLEARGFFEELDHPVCGRQNVAGLPFRFASVDRWLHRPAPTMGEHDH
                     EVLGGELGLSDDELARLTSAGIIGTAPSGL"
BASE COUNT          297 a          744 c          796 g          304 t
ORIGIN      
        1 cggtcgccgt ggcggtgcag ctcctcggcc aggcgcaggg ccaggacgtc acccacgagc
       61 tggatgagct ggatccgctc ctcggccacg atggtcagcg cccggaccgg gtcgaacgag
      121 cggtcgccgt ccaggaacag ggtgccgccg gtgtagaggg tggtcagagc cgtccactgg
      181 gccaggccgt gcatgagggg agccagcggc aggcgacggt cgactccggt gcctttccgg
      241 gcccacgctc cgatgtcggt cgggtcgtcg agacgaggga tgccccggcg gggcacgccc
      301 gcgccgccga tgacgccgaa gaagaggtcc tcgtgtcgcc acatgacgcc cttgggcatc
      361 cccgtggtcc cgccggtgta gagcaggtag aggtcgtcat tgctgcgatc gggtacggcg
      421 atccgctccg gcgaagcggc agccagggcc gcctcgtagt cgtcgccgcg aaccaggatg
      481 tgcggcggcg ggccggcctc gttcacccgg tcgaccaggt cggcctcggt gatgaccacg
      541 acgggtgagg cgtcctcgaa gtggtaacgg agctcctcgg ccacgtaccg gtggttgagg
      601 ttgatgggga tggcccgcag cttgtaggcg gccagcatcg cttcgatgta ctcggtgccg
      661 ttgtggacat ggcagccgat ggtcgagccg gcgacgacac cacagccctg caggtggtgg
      721 gccaggcggt tcgcccggtc ctcgagctcg gcgtgggtca ggcggcggtc cccgagggtc
      781 tcgctgcgga cgaccaccgc ctcccgctcg tcgcccaact cgtcgaccac gccttcccac
      841 aggtcggcca gctgatgggt cacggccctt tcatagcccc gacggcgccg tgccgattat
      901 tccggcggac gtcaggcggg ccagctcgtc gtcgctcaag cccagctcgc cgccgaggac
      961 ctcgtggtcg tgctcgccca tggtgggggc ggggcgatgc agccagcggt cgactgaagc
     1021 gaagcggaag ggcagcccgg cgacgttctg cctcccgcag accggatggt ccagttcctc
     1081 gaagaagcct cgggcctcga gctgggggtg ggtggcgatc gtccgggggt cacgggccgc
     1141 cccggcggga acgcctgcgg cgatcaggtc gtcgaccgtg gcctggaggt cacggtcggc
     1201 cgcccaggcc gccagctcgg cgtcgacctc gtcctcgtcg tcccggtcgg cggtgaggcc
     1261 ggtgacagcc accaacccgg cccactgctc gtcggtcgcc accgagaccg ccagccactg
     1321 ctcctcgccg gcgcacgggt agagcccctg gggcgagaac cccggcgccc ggttccccct
     1381 gcgctggagc tcgacgccgt tggccgtcca ctcgatgacc tgctcggcgg cgatgttgag
     1441 ggcggcctcc accatcgtcg actcgatgaa ggcgccggtg ccggtccgct cccgctcccg
     1501 tagggcggtc aggacggcga acgcggcgtg ggcgccggcc aacgggtcac aggggccgcg
     1561 tgggatccgg ggctggtcgt cgacatgacc ggtgatccag gccatgccgg tgacctgctc
     1621 catcgtctgg gcgaagccga cccggtcccg ccacgggccg tcgaggccga aggccggcat
     1681 ccggcagagc acgagtcggg ggttgagggc gtggaggcgc tccggcgtca gggcgaagcg
     1741 ctcggccacg cgggggctga agttctccaa caggacgtcg gcgtcggcca acaggcggtc
     1801 gagcaggtcg cgcccgtcgt ctgtggtcag gtccagcgtg atgccccgct tgccggtgtt
     1861 ggcggacagg gcgatggccg agcgctccca ccagcggggc tggtcgacga agagcccacc
     1921 ggccatgcgc atgccgtcgg gctggcgagt ggcctcgaga tgcaggacat cggcgcccag
     1981 cagagccagc agcctcccgg cctccggccc cgcccaccac gccgtggcgt cgatcacccg
     2041 cacccccgcc aacggcaggg ccgtcaacga cggttctgga accggaaact cgtcccccac
     2101 cgcgagttcc aggttccaga acgggggtgg ggtggcgccg a
//
LOCUS       DASTFV010000240         3934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_924376, whole genome shotgun sequence.
ACCESSION   DASTFV010000240 DASTFV010000000
VERSION     DASTFV010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924376"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFP02_04565"
     CDS             <1..211
                     /locus_tag="VFP02_04565"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET9542327.1"
                     /translation="SPRVATANVHGTGCSFASAVAAGLAQGHAPIDALGRAKAFIDRA
                     VRGGAAWRLGGGHGPIDHFGWGQSS"
     gene            193..1614
                     /locus_tag="VFP02_04570"
     CDS             193..1614
                     /locus_tag="VFP02_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542328.1"
                     /translation="MGAVLMITTTAAIVALVGTLAALAYVAHGAGSGKSLDVYLTARD
                     SQSTIRLSLAFLASGLGAWMLFAPPEVGQYIGVLGVIGYAVGGALPFVAYAWLGPKIR
                     DAAPDGVTLTDWVRERFGRAAQIWVGVVSVFYMFMFITAELTAIGDVFDLLAGIDPWV
                     PIVLAAGVTAAYTAWGGLPASLRTDYVISTMVLVLVGIAMAAILVHVDDPLAKAQDAG
                     LTSFSRAGWESIIVLSIAITAANLFHQGYWQRTWSAESSEVLTRAGYGSAVLSFLVLV
                     PFGVMGMIAGGVAAAEGVEPSPVPFFFLLDGLPEVIIFLVAVMSVALVASSVDSLQSA
                     LAATMARDLTDRRLTLYGAKIVTVLLTVPAVLIALQGYDILRLFLIADLVAAATVVPV
                     FLGLRRPASSASVVVGSLAGLASVVVLGVIQEGTLQGGIDLLTVASSPDLDVGSFILA
                     PLVSGLVAEIFVRLAPRQPTTVS"
     gene            complement(1607..2275)
                     /gene="pgl"
                     /locus_tag="VFP02_04575"
     CDS             complement(1607..2275)
                     /gene="pgl"
                     /locus_tag="VFP02_04575"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET9542329.1"
                     /translation="MFGELVVVDSVAAEFAERVIEAFIARPSDFFSLVLSGGQTARTC
                     YEALAEAGGAQIDWWRVDVYWGDERCVPLDHEDSNYRLVREALLENVGAVNANYPMRC
                     EDGPDRYHQLVGELGQFDVLHLGLGADGHTASLFPGSAALDADPGRLVVMNDDPTGAN
                     PHPRETLTFSGIERARLALVTVSGSDKAEALARTVAGETTASQIRAEQVVWLVDREAA
                     AQLS"
     gene            complement(2307..3398)
                     /gene="tal"
                     /locus_tag="VFP02_04580"
     CDS             complement(2307..3398)
                     /gene="tal"
                     /locus_tag="VFP02_04580"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HET9542330.1"
                     /translation="MTTKLQRLCAEQGQSPWLDNLRRGWITGGELQAWVDEGIRGITS
                     NPSIFQKAMGTGDDYDQQLAELAGQSIDDAYWALVVRDIEDALGILRPVYDSSDGLDG
                     FVSVEVAPALARDTDGTVAAARQLHQRIAEPNLYVKIPGTAEGIPAIRQMIAEGHSIN
                     VTLIFSLSRYEEVMEAYISGLEAYDGDLHPVSSVASFFVSRVDTEVDRRLDEIGTPEA
                     LALRGKAAVAQAQVAYDLFVATFGGPRWEALSARGARVQRPLWASTSTKNPAYPDILY
                     IDSLIGPETVNTIPDTTLAAFEDHGTVARTIDADPAGAKAVLEAVEAVGVDMEDVGQR
                     LEDEGVAAFEKAFDELLGTLGDKAEQLSA"
     gene            complement(3415..>3934)
                     /locus_tag="VFP02_04585"
     CDS             complement(3415..>3934)
                     /locus_tag="VFP02_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET9542331.1"
                     /translation="PDLCLIRPADANETAVAWRIAVDHDGPVALILTRQDLPVLEGTA
                     DGALDKGAYVLADPEGPPDLVLVGTGSEVSLCVAAADLLAAEGTACRVVSMPSWDLFA
                     EQGDAYRDEVLPPETPSLSVEAGVTMGWERWVDAAHGLDHFGASAPGAVAMDKLGFNP
                     EAVAESARRLLA"
BASE COUNT          511 a         1394 c         1402 g          627 t
ORIGIN      
        1 cagcccacgc gtggccaccg ccaacgtgca cgggacgggc tgctcgttcg cgtcggccgt
       61 cgccgccggg ctggcccagg gtcacgcgcc catcgacgcg ctggggcggg ccaaggcctt
      121 catcgaccgc gcggtgcgcg gcggggcggc gtggcggctc ggtggtggcc acgggccgat
      181 cgaccacttc ggatgggggc agtcctcatg atcaccacca ccgccgccat cgtggccctc
      241 gtcggcaccc tggcggcgct cgcgtacgtc gcccacgggg ccggcagcgg caagagcctg
      301 gacgtctatc tgaccgcccg ggacagccag tccacgatca ggttgtcgct ggcgttcctg
      361 gcctccgggc tcggcgcctg gatgctcttc gccccgcccg aggtgggcca gtacatcggg
      421 gtcctgggcg tgatcggcta cgccgtcggc ggggcgctgc ccttcgtggc gtacgcgtgg
      481 ctcgggccga agatccgcga cgccgccccc gacggcgtga ccctcaccga ctgggtccgt
      541 gagcgcttcg gtcgtgccgc ccagatctgg gtcggggtgg tctcggtctt ctacatgttc
      601 atgttcatca ccgccgagct caccgccatc ggcgacgtgt tcgatctcct cgccggaatc
      661 gatccgtggg tgccgatcgt cctggccgcc ggggtcaccg ccgcctacac ggcgtggggt
      721 ggcctgcccg cctccctgcg cacggactac gtgatcagca cgatggtcct cgtgctcgtc
      781 ggcatagcca tggccgccat tctcgtccac gtcgacgacc cgctggccaa ggcccaggac
      841 gccggtctga cctccttctc ccgggccggc tgggagagca tcatcgtgtt gagcatcgcc
      901 atcaccgccg ccaacctgtt ccaccagggc tactggcagc ggacgtggtc ggcggagagc
      961 tccgaggtgc tgacccgagc cgggtacggc agcgccgtcc tgagcttcct cgtgctcgtg
     1021 cccttcggcg tgatgggcat gatcgcgggg ggcgtggccg ccgccgaggg ggtcgagccc
     1081 tcgcccgtgc ccttcttctt cctcctcgac ggcctgcccg aggtgatcat cttcctcgtg
     1141 gccgtgatgt ccgtggccct ggtcgcgtcc agcgtggaca gcctccagag cgccctggcg
     1201 gcgacgatgg cccgcgacct gaccgaccga cgtctgaccc tctacggggc aaagatcgtg
     1261 acggtcctgc tgaccgtgcc cgccgtgttg atcgccctgc agggctacga catcctccgc
     1321 ctgttcctca tcgccgacct ggtggcggcg gccaccgtgg tcccggtgtt cctcggcctc
     1381 cggcggcccg cgtcgagcgc gtcggtcgtc gtcggctccc tggccgggct ggcctcggtc
     1441 gtggtgctcg gcgtgatcca ggagggcacg ctccagggcg gcatcgacct gctgacggtg
     1501 gccagctcgc ccgacctcga cgtgggctcg ttcatcctgg ccccgctcgt ctccggcctg
     1561 gtggccgaga tcttcgtccg cctggccccg aggcaaccga cgacggtcag ctgagctgag
     1621 cggcggcctc gcggtcgacc agccacacca cctgctcggc ccggatctgg gaggcggtgg
     1681 tctcgcccgc gaccgtgcgg gccagggcct cggccttgtc ggacccgctc accgtgacca
     1741 gggcgaggcg ggctcgttcg atgccgctga aggtgagcgt ctcccgaggg tgggggttgg
     1801 cgcccgtggg gtcgtcgttc atgaccacca ggcgaccggg atcggcgtcc agggcggccg
     1861 agccggggaa gagggaggcg gtgtgaccgt cggcgccgag gccgaggtgg agcacgtcga
     1921 actggccgag ctcgcccacc agctggtgat accggtcggg cccgtcctca cagcgcatgg
     1981 ggtagttggc gttgaccgcc cccacgttct cgagcagggc ctcacggacc agtcggtagt
     2041 tcgagtcctc gtggtccagg ggcacgcagc gttcgtcgcc ccagtagaca tccacccgcc
     2101 accagtcgat ctgggcaccg ccggcttcgg ccagggcctc gtagcaggtg cgagccgtct
     2161 ggccgccgga cagaaccagc gagaagaagt ccgaggggcg ggcgatgaag gcctcgatga
     2221 cgcgctcggc gaactccgcc gccacgctgt ccacgaccac caactccccg aacacggccc
     2281 cgacgttagg cgacgaggtc accgcatcag gcgctgagct gttccgcctt gtcgccgagg
     2341 gtgccgagga gctcgtcgaa ggccttctcg aacgcagcca cgccctcgtc ctcgaggcgc
     2401 tggcctacgt cctccatgtc gacgccgacg gcttcgacgg cctccagtac cgccttggcc
     2461 cccgcggggt cggcgtcgat cgtccgagcc acggtgccgt ggtcctcgaa ggcggccagg
     2521 gtggtgtccg ggatggtgtt gaccgtctcc gggccgatga gggagtcgat gtacaggatg
     2581 tcgggatacg ccgggttctt ggtcgaggtc gaggcccaga gcggacgctg gacccgcgcc
     2641 ccccgggcgg acagcgcctc ccatcgtggt cccccgaagg tggccacgaa gaggtcgtag
     2701 gcgacctggg cctgcgccac ggcggccttg ccccgcaggg ccaacgcctc gggcgtcccg
     2761 atctcgtcga ggcgccggtc gacctccgtg tcgacccgac tgacgaagaa cgaggccacg
     2821 ctgctgaccg gatggaggtc gccgtcgtag gcctcgaggc cgctgatgta ggcctccatg
     2881 acctcctcgt agcggctcag cgagaagatc agggtcacgt tgatgctgtg gccctcggcg
     2941 atcatctggc ggatcgccgg gatgccctcg gccgtgcccg ggatcttcac gtagaggttg
     3001 ggctcggcga tgcgctgatg gagctggcgg gctgcggcga cggtgccgtc ggtgtcccgg
     3061 gccagcgccg gtgccacctc gaccgacacg aagccgtcca agccgtcact gctgtcgtag
     3121 accgggcgca ggatgccgag ggcatcctcg atgtcccgga cgacgagggc ccagtaggcg
     3181 tcgtcgatgc tctgaccggc cagctcggcc agctgctggt catagtcgtc gccggtgccc
     3241 atggccttct ggaagatgga gggattcgag gtgatgcccc ggatcccctc gtccacccag
     3301 gcctggagct cgccgccggt gatccaaccc cgccgcaggt tgtccagcca cggactctgc
     3361 ccctgctcgg cgcacagacg ctgaagcttg gtcgtcatgg gggtgccctt tcggtcaggc
     3421 gaggaggcgg cgggccgact cggccaccgc ctcggggttg aagccgagct tgtccatggc
     3481 caccgctccc ggggccgaag ccccgaagtg gtcgaggccg tgagccgcgt ccacccagcg
     3541 ctcccagccc atggtgacgc cggcctccac ggagagcgat ggcgtctccg ggggcagcac
     3601 ctcgtcgcgg taggcgtcgc cctgttcggc gaagaggtcc cacgacggca tcgacacgac
     3661 gcggcacgcc gtgccttcgg cggccagcag gtcggcggcg gcgacacaga gggagacttc
     3721 gctgccggtc ccgaccagga cgaggtcggg cgggccctcg gggtcggcga gcacgtaggc
     3781 gcccttgtcc agggccccat ccgccgtgcc ctcgagcacg gggaggtcct gccgggtcag
     3841 gatcaaggcg accggaccgt cgtggtcgac ggcgatgcgc cacgccaccg cggtctcgtt
     3901 ggcgtcggcc ggacggatga ggcagaggtc gggg
//
LOCUS       DASTFV010000241         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_925249, whole genome shotgun sequence.
ACCESSION   DASTFV010000241 DASTFV010000000
VERSION     DASTFV010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925249"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VFP02_04590"
     CDS             complement(<1..824)
                     /locus_tag="VFP02_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET9542332.1"
                     /translation="MDLELGEDERALQDGLRSFLDGRFPMAAVRAQADGLDRALWREL
                     ADTGVFGLRLPEADGGVGLGMTEAVLVFEELGRSLVAGPVLATHLAAALVTGAASGDT
                     IVGLIERPADVVLVEHLDALDVLLAIDDDGIWTIDPASLTAEPVARPLDPLTPLHRVV
                     DLPQGERLAGADEASTWHRQGATLVAAMLLGMAARSTELAVAYAGEREQFGKPISGFQ
                     LTQQKLVDMNLEYTKGVLLALHLGRRKDAGTLRPEQVSLGKLNNVREALDICRTART"
     gene            complement(824..2011)
                     /locus_tag="VFP02_04595"
     CDS             complement(824..2011)
                     /locus_tag="VFP02_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9542333.1"
                     /translation="MDLRYTDDDVAFRDELRDWLAEVMPTVGPAPDEDDWAATRAYDA
                     AWQRMLFDAGYAGINWPTEYGGRGASPVEHLIFLEETERVGAPYVGTNFVGLLHAGPT
                     IMTEASDAQKQFHLPAILKGEHCWCQGFSEPQAGSDLASLRTRAVQDGDEYVINGQKI
                     WTSHAHVAQYCEMLVRTDPEAPKHRGISWLILPMDTPGIDIRPLKTIRGGSEFSEVFL
                     DDVRVPVANRVGAENDGWRVAMVTFSFERGTAFVSELLYSMQLVADLAAVAKQVTSHG
                     AAAWEDRGLRREIGHVAAELDALWALTRRNISQAARDGVPGVGGSVFKLAYSEVRQKL
                     GDLTDRVLARAALSIDDLPGMGNGRHVQGRLHALSSSIAAGTTQIQRNIVGERVLGLP
                     KER"
     gene            complement(2069..>2754)
                     /locus_tag="VFP02_04600"
     CDS             complement(2069..>2754)
                     /locus_tag="VFP02_04600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET9542334.1"
                     /translation="FIAIEHKDGPYSVYKHRLAAHVDVGDVVERGQVIGQVGSSQAPR
                     VHLHYDEQATLVDFNRPGNRLPLGPMYYWSGGRRRAPGSIGGATEWSGLPRGTRITST
                     AWRTGWPLGVSTPRQFRSALAGLGTADRVGPFVIRLEADIPLSGGPLEYRGDDLLIVL
                     GRRFSLRRDNGCRVIETIGPLELNNVRLGPAQGGCAAVFSTRLVTVVGNGIHPRQLDA
                     GGGATFVSS"
BASE COUNT          413 a          966 c          962 g          413 t
ORIGIN      
        1 gtgcgcgcgg tacggcagat gtcgagcgct tcacgcacgt tgttgagctt gccgagactg
       61 acctgctcgg ggcgaagggt gccggcgtcc ttacggcgcc ccaggtgcag tgccagcagc
      121 acgcccttgg tgtactccag gttcatgtcc accagcttct gctgggtgag ctggaacccg
      181 gagatcggct tgccgaactg ctctcgctca ccggcgtacg ccaccgccaa ctccgtgcta
      241 cgggcggcca tgcccaggag catcgccgcg accagcgtgg ccccctggcg gtgccaggtg
      301 ctcgcctcat cggccccggc gagccgctcg ccctggggca ggtcgacgac gcggtgcagc
      361 ggggtcagcg ggtcgagcgg ccgggccacc ggctcggcgg tgaggctggc cgggtcgatc
      421 gtccagatgc cgtcgtcgtc gatcgccagg aggacgtcga gggcgtcgag gtgctcgacg
      481 agcacgacgt cggcgggccg ttcgatcaac cccacgatcg tgtccccgga ggcggccccg
      541 gtgaccagag cggcggccag gtgcgtcgcg aggaccgggc cggccaccag cgaccgtccc
      601 agctcctcga agacgagcac cgcttcggtc atgccgaggc cgacgccacc gtccgcctcc
      661 ggcaggcgga ggccgaacac cccggtgtcg gccagctccc gccagagcgc ccggtccagc
      721 ccgtcggcct gggcccgcac ggcggccatg gggaaccggc cgtccaggaa cgaccgcagc
      781 ccgtcctgga gcgcccgctc gtcctcaccc agctcgaggt ccatcagcgt tccttgggca
      841 ggccgaggac ccgctcgccc acgatgttgc gttggatctg ggtcgtcccg gcggcgatcg
      901 agctggagag ggcatggagt cggccctgca cgtggcggcc gttgcccatg ccggggagat
      961 cgtcgatcga cagggccgcc cgggccagca cccgatcggt caggtcgccc agcttctggc
     1021 ggacctccga gtacgccagc ttgaacaccg acccgccgac gccgggcacg ccgtcccggg
     1081 ccgcctggct gatgttgcga cgggtcaggg cccagagggc gtccagttcg gcggcgacgt
     1141 ggccgatctc gcgccgcagg ccccggtcct cccaggcggc ggcgccgtgg ctcgtcacct
     1201 gcttggcaac ggcggccagg tccgccacga gctgcatgga gtagagcaac tcgctgacga
     1261 acgccgtgcc ccgctcgaag ctgaaggtga ccatggccac ccgccagccg tcgttctcgg
     1321 cgccgacccg gttggccacc gggacccgga cgtcgtcgag gaagacctca ctgaactccg
     1381 agccgccccg gatcgtcttg agcggacgga tgtcgatgcc cggcgtgtcc atggggagga
     1441 tcagccagga gatgccccga tgcttcgggg cctccgggtc cgtgcggacg agcatctcgc
     1501 agtactgggc gacgtgggca tggctggtcc agatcttctg gccgttgatc acgtactcgt
     1561 cgccgtcctg cacggccctc gtgcggaggc tggccaggtc ggagccggcc tggggctcgg
     1621 agaacccttg gcaccagcag tgctccccct tgaggatggc gggcaggtgg aactgcttct
     1681 gggcgtcgga ggcctcggtc atgatcgtcg gcccggcgtg gagcaggccc acgaagttgg
     1741 tgcccacgta gggggccccg acccgctcgg tctcctccag gaagatcagg tgctcgaccg
     1801 gcgaagcccc ccgaccgccg tactccgtcg gccagttgat cccggcgtag cccgcgtcga
     1861 agagcatgcg ctgccaggcc gcgtcgtagg cccgggtagc ggcccagtcg tcctcgtccg
     1921 gggccgggcc gacggtgggc atgacctcag ccagccagtc ccggagctcg tcccggaagg
     1981 cgacgtcgtc gtcggtgtac cggagatcca tcgagccgca ggttaggggc ggacctgacg
     2041 acccgtcaga tccgtggccc gccggtgctc aagagctgac gaacgtggcg ccgccccctg
     2101 catcgagttg cctcggatgg atcccgttgc caaccaccgt gacgaggcga gtggagaaca
     2161 cggctgcgca gccgccctgt gccggaccca gccggacgtt gttcagctcg agtggtccga
     2221 tcgtctcgat gacccgacac ccgttgtcac ggcggagtga gaaccttcgg ccgagaacga
     2281 tgaggaggtc atcgccgcgg tactcgagag gaccccccga gagagggatg tcggcctcga
     2341 gtcgtatgac gaacggcccg actcggtctg ctgtccccag tccagccaga gccgatcgga
     2401 actggcgagg ggtcgagaca cccaggggcc aaccggttcg ccaagccgtc gacgtgattc
     2461 gagtgccgcg cggcagtccg gaccactcgg tcgcgccacc gatcgaaccc ggcgcccttc
     2521 tacggccgcc cgaccagtag tacatcggtc cgagcggaag gcgatttccg gggcgattga
     2581 agtcgacgag cgtcgcctgc tcgtcatagt gcagatgcac tctcggcgcc tgggatgacc
     2641 cgacctggcc gatgacctgc cctcgctcga ccacatcgcc cacatcgacg tgggccgcca
     2701 agcggtgctt gtacacgctg tacggaccgt ctttgtgctc gatggcgatg aaca
//
LOCUS       DASTFV010000242         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_927223, whole genome shotgun sequence.
ACCESSION   DASTFV010000242 DASTFV010000000
VERSION     DASTFV010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927223"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /gene="glyS"
                     /locus_tag="VFP02_04605"
     CDS             complement(<1..729)
                     /gene="glyS"
                     /locus_tag="VFP02_04605"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HET9542335.1"
                     /translation="MAPPADAQPDAPDDRMQKIVNLSKRRGFVFPSAEIYGGFRSAYD
                     YGPIGVLLLRNVKDAWWRSMVQLRTDVVGLDAAILSPPAVWEASGHLANFTDPLVDCR
                     ECQSRWRLDKLDDPDVCPSCGARDSFTEARAFNLMFKTHAGPVEGEGNDVYLRPETAQ
                     GHFVNFTNVLQASRKKPPFGIAQVGKSFRNEITPGNFIFRTREFEQMEMEYFVPPDEG
                     QKWFEYWCEERMRWYTDLGIPAEDL"
     gene            803..1672
                     /locus_tag="VFP02_04610"
     CDS             803..1672
                     /locus_tag="VFP02_04610"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET9542336.1"
                     /translation="MTRRPLTDPTAVVARRGGAWFIDLVLCGLAGAVPALLLADAYSG
                     NEADGGAPVQWIEGDLAVFIRDTTIVLRGPDLAITLGAFAVAVLLFLVLLPGRKGWSP
                     GYLAADLRLQRRDGERAGIGRALVRTIAWVIDILPGLPLVAYTSASLTRRHQRVGDLL
                     ARTYVVDKRTAGRPVDAPVEDDLTAEVLEPERLIEPVPEVVVAPVPEPLVTPAPAPEP
                     VPVAAAVAAGTGPPDGVPPDQPIWDRRQKRYVLWHSEAGRWLEHGATGWAPLDRDGER
                     DTSRRDGGARQEG"
     gene            1669..>2148
                     /locus_tag="VFP02_04615"
     CDS             1669..>2148
                     /locus_tag="VFP02_04615"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET9542337.1"
                     /translation="MSDSTRDKPTAVLGRRYLAYLIDLIVHLAVVAVPFFLLGEKAAD
                     SADTSPFVDAYRLNPFYAVRVDDQVWLFTKNDLIAIGAVSAAFVILVTVIIQGRVGWT
                     IGKALTGLRTVNRDGGPAGILRALGRTILLVIDAIPSWLIPIVGPLIALVTSDNRRLG
                     "
BASE COUNT          306 a          773 c          738 g          331 t
ORIGIN      
        1 caggtcctcc gccgggatgc cgaggtcggt gtaccagcgc atccgttcct cgcaccagta
       61 ctcgaaccac ttctggccct cgtcgggcgg cacgaagtac tccatctcca tctgctcgaa
      121 ctcgcgggtg cggaagatga agttgcccgg ggtgatctcg ttgcggaagg acttgcccac
      181 ctgcgcgatg ccgaacggcg gcttcttgcg gctggcctgg agcacgttgg tgaagttgac
      241 gaagtggccc tgggccgtct cggggcggag gtagacgtcg ttcccctccc cctccaccgg
      301 gccggcgtgg gtcttgaaca tcaggttgaa cgcccgggct tcggtgaaac tgtcccgggc
      361 gccgcagctc gggcagacgt cgggatcgtc gagcttgtcc aggcgccacc gggactggca
      421 ttcgcggcag tcgaccagcg gatcggtgaa gttggccagg tgcccggagg cctcccacac
      481 ggcgggcggc gagaggatgg cggcgtccaa gccgaccacg tcggtgcgca gctggaccat
      541 ggagcgccac cacgcgtcct tgacgttgcg caggagcagg acgccgatgg ggccgtagtc
      601 gtaggccgag cggaagccgc cgtagatctc ggccgagggg aacacgaaac cccggcgctt
      661 ggagaggttg acgatcttct gcatccggtc gtcgggcgcg tcaggctggg cgtcggcggg
      721 gggagccatg gcccggatcg taccggcgac catcggtccg cccgggggct ttggtgggag
      781 gtcacgatgc taggaaggga cggtgacacg gcgccccctc accgacccga ccgcggtggt
      841 cgcccgccgg ggtggggcgt ggttcatcga cctggtcctg tgtggcctgg cgggcgccgt
      901 gcccgccctc ctgctggccg acgcctactc cggcaacgag gccgacggag gcgccccggt
      961 ccagtggatc gagggtgacc tcgccgtctt catccgggac accaccatcg tgctccgggg
     1021 cccggacctc gccatcaccc tcggcgcctt cgcggttgcg gtcctgctct tcctcgtcct
     1081 gctcccggga cgcaagggct ggtccccggg gtacctggcc gccgacctcc gcctccagcg
     1141 ccgcgacggt gagcgggccg ggatcggacg ggccctggtc cggaccatcg cctgggtcat
     1201 cgacatcctc cccggcctac ccctggtggc ctacacgtcg gccagcctca cgcgccgcca
     1261 ccaacgcgtg ggtgacctcc tggcccggac ctacgtcgtc gacaagcgaa ccgcgggccg
     1321 accggtagac gcccccgtcg aggacgacct caccgctgag gtgctcgagc ccgagaggct
     1381 gatcgagccc gtgcccgagg tcgtcgtcgc accggtcccc gaacccctcg tgacgccggc
     1441 gccggctccc gagccggtgc ccgtggccgc ggccgtcgcc gccggaacgg gcccgcccga
     1501 cggggtgcct ccggaccagc ccatctggga ccgacggcag aagcgctacg tgctctggca
     1561 ctccgaggcc ggccgttggc tcgagcacgg cgccaccggc tgggccccgc tggaccggga
     1621 tggcgaacga gacacgagtc ggagggacgg aggggctaga caggaagggt gagcgacagc
     1681 acccgagaca agccgacggc ggtcctggga aggcggtacc tcgcctacct catcgacctg
     1741 atcgtgcatc tcgccgtggt ggccgtgccc ttcttcctgc tcggcgagaa agctgccgac
     1801 tcggctgaca cgagcccgtt cgttgacgcc taccgactca acccgttcta cgcggtccgc
     1861 gtcgacgacc aggtctggct cttcaccaag aacgacctca ttgccatcgg cgccgtctcg
     1921 gccgccttcg tcatcctggt cacggtcatc atccagggcc gcgtcggctg gaccatcggc
     1981 aaggccctga ccggcctgcg cacggtgaac cgggacggtg gccccgcagg gatcttgcgg
     2041 gccctcggac gcacgatcct cctcgtcatc gacgccatcc ccagctggtt gatccccatc
     2101 gtcgggccgc tgatcgccct cgtcacctcc gacaaccgac gcctcggc
//
LOCUS       DASTFV010000243         3256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_929685, whole genome shotgun sequence.
ACCESSION   DASTFV010000243 DASTFV010000000
VERSION     DASTFV010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929685"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VFP02_04620"
     CDS             <1..693
                     /locus_tag="VFP02_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET9542338.1"
                     /translation="LAGIIRARATGEGCQMELAQSDAAAYMDWYRIETWLAYNRPEDE
                     VTGNASDDYERRAPGLAGMREGVRYQLYESADGHVLFMASEQAFWKNFCEGIDRADLF
                     ERWPGSKFADHAKGNLELQAVLKEVFLSRTSAEWLAFADDHNVPLAPVNTAKTIVDDA
                     QFQHRFPLYGVDQLGAEQLPLPLHLVGEDLPVPTKAPEVGQDTDAVLRDVLGYDDTRI
                     AELRESGTFGSD"
     gene            795..1601
                     /locus_tag="VFP02_04625"
     CDS             795..1601
                     /locus_tag="VFP02_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HET9542339.1"
                     /translation="MAQVLTERDGHTMVVTLNRPHRLNAMTMTMFAMLDDAWHEAADD
                     PDIRTIILTGAEGNFSSGMDLRSLAGDSDEDDALSQDEINQKLADDVDFIYRGLLKTY
                     QPPKPVIAAVEGNAIAGGTEILQGTDIRVAGDTATFGISEARWSLYPAGGSAVRHRRQ
                     IPYTIAAEILLTGKHMTAQETRDIGLIGHVVPAGEALAKAREIAEVINFNGPLAVEAI
                     LRTLRETETLSEEEAFAHEMTYGMAVMGSEDAKEGPRAFKEKRRPNFQRK"
     gene            1613..2782
                     /locus_tag="VFP02_04630"
     CDS             1613..2782
                     /locus_tag="VFP02_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9542340.1"
                     /translation="MPGQHEPLTEAMVEPGFNGRDPFPHYARLRAEAPVAWNATQGFW
                     ALSRFAEVHEVSTDPDRFCSGKGILTMEIGLEYDSPPTMMHTDPPDHTAYRRLVQPGF
                     KPSLMKALVPVARDRLLDLFDGVQDGQPVDFVQAVSVPFPLRIIGDLLGLPDEDWEEF
                     FYWSEAMTGALDLTPEQRTEIQGDMRAYFFEVIGRKRAEPEEDLISVLAAEDLSDDEL
                     FMFLNQLLVAGNETTRNTISGGLWALAERPDQWQRLVEDRSLVPSAVEEILRWTTAVI
                     AFMRTATRDTTLGGVEIAANDPVLMLYSSANRDEAQFGPTADQFDVGREPNNHLAFGF
                     GAHFCIGAALARLEVRLVLEELLARFRTLEPAGEVAYSPSDIIAGVTSAPLRFAV"
     gene            2792..>3256
                     /locus_tag="VFP02_04635"
     CDS             2792..>3256
                     /locus_tag="VFP02_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9542341.1"
                     /translation="MDQFEGKTALITGAASGIGRAMAERFAAEGMQLVLVDVDEPGLE
                     QTVADLGGEAHVVDVTDTEALEALSRNVPVVHVLCPNAGVAPVGPVLEMDKATWDWLI
                     GVNLQQVVHLTRLYGPRMVEQGDGHIVYTASGAGILAVPTLGPYCVTKHGVVA"
BASE COUNT          538 a         1193 c         1067 g          458 t
ORIGIN      
        1 ctggccggga tcatccgggc tcgggccacc ggtgagggct gccagatgga gctggcccag
       61 tccgacgccg ccgcctacat ggactggtac cggatcgaga cctggctggc ctacaaccgc
      121 cccgaggacg aggtcaccgg caacgcctcc gacgactatg agcgccgggc gccggggctg
      181 gccggcatgc gcgagggcgt gcgctaccag ctctacgagt cagccgacgg ccatgtcctc
      241 ttcatggcct ccgaacaggc cttctggaag aacttctgcg agggcatcga ccgagccgac
      301 ctgttcgagc gctggcccgg ctccaagttc gccgaccacg ccaagggcaa cctcgagctg
      361 caggccgtgc tcaaggaggt cttcctgtcc aggacctcgg cggagtggct ggccttcgcc
      421 gacgaccaca acgtgcccct ggccccggtc aacacggcca agaccatcgt cgacgacgcc
      481 cagttccagc accgcttccc gctctacggg gtcgaccagc tcggcgccga gcagctcccc
      541 ctccccctcc acctcgtcgg cgaggacctc cccgtgccca ccaaggcccc cgaggtcggt
      601 caggacaccg acgccgtcct ccgcgacgtc ctcggctacg acgacacccg catcgccgag
      661 ctgcgtgaat ccggaacctt cggttccgac taaaacgacg ctgcggagca gagcgaagca
      721 gcactcccac cagttcgtcg gaagctgccg taggcagcgg gagccgcgca ggcggcgaca
      781 ggaggcatgg cctcatggct caggtactga ccgagcggga cggtcacacg atggtcgtga
      841 ccctcaaccg cccccatcgc ctgaacgcga tgacgatgac gatgttcgcg atgctcgacg
      901 acgcctggca cgaggcggcc gacgacccgg acatccggac gatcatcctc accggggccg
      961 agggcaactt ctcctccggc atggacctgc gctccctggc tggggacagc gacgaggacg
     1021 acgccctgtc ccaggacgag atcaaccaga agctggccga cgacgtcgac ttcatctacc
     1081 gggggctgct caagacctac cagccgccga agccggtgat cgccgccgtc gagggcaacg
     1141 ccatcgccgg cgggaccgag atcctgcagg gcaccgacat ccgggtggcc ggggacacgg
     1201 cgaccttcgg catctccgag gcccggtggt cgctgtatcc ggccggcggc tcggcggtcc
     1261 gccaccgccg tcagatcccc tacacgatcg ccgccgagat cctgctgacc ggcaagcaca
     1321 tgaccgccca ggagaccagg gacatcggcc tgatcggcca cgtcgtcccc gccggcgagg
     1381 ccctggccaa ggcccgggag atcgccgagg tcatcaactt caacggcccg ctggccgtcg
     1441 aggccatcct ccggaccctg cgggagaccg agacgctctc ggaggaggag gccttcgccc
     1501 acgagatgac ctacggcatg gccgtcatgg gctccgagga cgccaaggag ggccctcggg
     1561 ccttcaagga gaagcgccgg ccgaacttcc agcggaagta aaggtccggg ggatgccagg
     1621 tcaacacgag ccactgaccg aggcgatggt cgagcccggc ttcaacggcc gagacccgtt
     1681 cccgcactac gcccgactgc gggccgaggc gcccgtggcc tggaacgcca cccaggggtt
     1741 ctgggccttg agccgcttcg ccgaggtgca cgaggtgagc accgaccccg accgcttctg
     1801 ctcgggcaag gggatcctca ccatggagat cgggctcgag tacgacagcc cgcccacgat
     1861 gatgcacacc gacccgccgg accacacggc gtaccgaagg ctggtgcagc ccggcttcaa
     1921 gccctcactg atgaaggcgt tggtgcccgt ggcccgggac cgcctgctcg acctcttcga
     1981 cggggtgcag gacggccagc ccgtcgactt cgtccaagcc gtgtcggtcc ccttcccgct
     2041 gcggatcatc ggcgacctgc tcggtctccc ggacgaggac tgggaggagt tcttctactg
     2101 gtccgaggcg atgaccgggg ccctcgacct cacccccgag cagcgcacgg agatccaggg
     2161 cgacatgcgg gcgtacttct tcgaggtcat cggccggaag cgggccgagc ccgaagagga
     2221 cctgatctcg gtcctcgccg ccgaggacct gagcgacgac gagctcttca tgttcctcaa
     2281 ccagttgctc gtcgccggca acgagaccac gcgcaacacg atctccggcg ggctctgggc
     2341 gctggccgag cggcccgacc agtggcagcg gctggtcgag gaccgatcgc tcgtgccctc
     2401 cgccgtcgag gagatcctgc gctggaccac cgcggtgatc gccttcatgc gcaccgccac
     2461 ccgggacacc acgctgggtg gcgtcgagat cgcggccaac gaccccgtgt tgatgctcta
     2521 ttcctcggcc aaccgggacg aggcccagtt cggacccacc gccgaccagt tcgacgtggg
     2581 ccgcgaaccc aacaaccacc tggcgttcgg cttcggcgcc cacttctgca tcggggcggc
     2641 gctggcccga ctggaggtcc ggctcgtgct cgaggagctg ctggcccgct tccgcacgct
     2701 cgagccggcg ggcgaggtcg cctactcccc ctccgacatc atcgccgggg tcacctcggc
     2761 cccgttgcgc ttcgccgttt aggaggctcc catggaccag ttcgaaggca agaccgcgct
     2821 catcaccggc gcggccagtg ggatcgggcg ggccatggcc gagcggttcg cggcggaagg
     2881 catgcagctg gtgctggtcg acgtcgacga gcccgggctc gagcagaccg tcgccgacct
     2941 cggaggcgag gcccatgtcg tcgacgtcac cgacaccgag gccctcgagg ccctgtctcg
     3001 caacgtgccc gtcgtccacg tgctgtgccc caacgccggc gtcgcccccg tcggtcccgt
     3061 gctcgagatg gacaaggcga cctgggactg gctgatcggg gtcaacctcc agcaggtcgt
     3121 gcacctgacc cggctctacg ggccccgtat ggtcgagcag ggcgacggcc acatcgtcta
     3181 cacggcgtcc ggcgcgggga tcctggccgt gcccaccctc ggcccgtact gcgtgaccaa
     3241 gcacggggtc gtcgcc
//
LOCUS       DASTFV010000244         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_931344, whole genome shotgun sequence.
ACCESSION   DASTFV010000244 DASTFV010000000
VERSION     DASTFV010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931344"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1892)
                     /locus_tag="VFP02_04640"
     CDS             complement(99..1892)
                     /locus_tag="VFP02_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9542342.1"
                     /translation="MQVTFWGTRGSIAKAGPSTLRFGGNTSCVEVRSDAGTIVVLDCG
                     TGAHGLGEDIVARNGGEPMEGHLLIGHTHWDHIQGLPFFAPLFQPGSSWHIYGPRGLA
                     GSLSEILAGQMQYAYFPVSIEQLTATVDYHDLIEGTFDIDDITVTSRYLNHPALTLGY
                     RLEVDGATVVYSTDHEPHDRRLAAGGDPATSRHDLDHAGLFAGADLLIHDAQYVAEEY
                     PDKVGWGHSTVEYVVSVAHQAGVKQVALFHHDPSRTDEAVEEVVAEARAFASELGFAG
                     EVIAAAEGETIELRGTSTGVQGAARPIPKPRLEHMARSVLLAVRTPEIATILREAATE
                     EDLEVWETTDPAEAVEIARRERPAIVVLEDQEDSEVLAEFAHAIDDLEKWPGGDISLI
                     AVTATGHPPTCGDDVAITDSLEWPASPGYVRTKLRAWLLRRACRWQTAPLPQDEAARL
                     QALHDLEVLDTDPEPRFDQYTDLASSTFDVPVALITLIDEDRQWFKSRHGIDVAETPR
                     DMAVCAYTILGDDVFQVPDAFADARFADNPLVASGPRFRFYAGAPLTLSNGLRAGTLC
                     VVDHRPRLLDDDQLAELARLAGLVTAELESD"
     gene            1982..>2215
                     /locus_tag="VFP02_04645"
     CDS             1982..>2215
                     /locus_tag="VFP02_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542343.1"
                     /translation="MHEPGVHTGGGGWRSLWRGRVDVDPQLPALLGRILDRLGRYLDE
                     GGLDAPTWVTDMAALERQLTYLAGGTRDRALHSL"
BASE COUNT          308 a          740 c          846 g          321 t
ORIGIN      
        1 aaggccaacg ccaccaccac ggccaacgcc agccccaccg gaccagcgtg tcacctgacc
       61 cgcgccccgg cccgatgggc caccgcccag ggctgcgctc agtcgctttc gagctcggcg
      121 gtgaccaggc cggcgaggcg ggccagctcc gccagctggt cgtcgtcgag cagccgggga
      181 cggtggtcga cgacacagag ggtcccggcc cgcaggccgt tcgacagggt caggggggca
      241 cccgcgtaga accggaagcg ggggccggac gccacgagag ggttgtcggc gaagcgggca
      301 tcggcgaagg catcggggac ctggaacacg tcgtcgccga ggatggtgta ggcgcagacg
      361 gccatgtcgc ggggggtctc ggcgacgtcg atgccgtgcc tcgacttgaa ccactggcgg
      421 tcctcgtcga tcagggtgat cagggcgacg gggacgtcga aggtcgagct ggccaggtcg
      481 gtgtactggt cgaagcgagg ctcggggtcg gtgtcgagca cctcgaggtc gtgcagggcc
      541 tgcaggcggg cggcctcgtc ctgggggagg ggtgcggtct gccaccggca ggcccgacgc
      601 agcagccagg cccgcagctt ggtgcgcaca tacccggggc tggcgggcca ctccagcgag
      661 tcggtgatgg cgacgtcatc accgcacgtc ggggggtggc cggtggcggt gacggcgatc
      721 aggctgatgt cgccgcccgg ccacttctcc aggtcatcga tggcgtgggc gaactcggcc
      781 agcacctcac tgtcctcctg gtcctcgagg acgacgatcg ccgggcgctc ccgccgggcg
      841 atctcgaccg cctcggcggg gtcggtggtc tcccagacct cgaggtcctc ctccgtcgcc
      901 gcttcccgca ggatcgtggc gatctcgggg gtgcgtacgg cgagcaggac gctgcgggcc
      961 atgtgctcga gccgcggttt ggggatcggc cgggccgcgc cctggacgcc ggtcgaggtc
     1021 ccccgcagct cgatcgtctc cccctcggcg gcggcgatga cctcgccggc gaagcccagc
     1081 tcggacgcga aggcccgggc ctcggccacg acctcctcaa cggcctcgtc ggtccgggac
     1141 gggtcatggt ggaacagggc gacttgcttc acccccgcct ggtgggcgac cgagacgacg
     1201 tactcgacgg tgctgtggcc ccaacccacc ttgtcggggt actcctcggc gacgtactgg
     1261 gcgtcgtgga tcagcaggtc ggcacccgcg aagaggccgg cgtggtcgag gtcgtggcgg
     1321 ctggtcgccg ggtcgccgcc cgcggcgagg cgccggtcgt ggggctcgtg gtcggtggag
     1381 tagacgaccg tggccccatc gacctcgagg cggtagccga gggtgagcgc cgggtggttc
     1441 aggtagcggc tggtgaccgt gatgtcgtcg atgtcgaagg tgccctcgat caggtcgtgg
     1501 tagtcgacgg tggccgtgag ctgttcgatg gagaccggga agtaggcgta ctgcatctgc
     1561 ccggcgagga tctcgctgag cgatccggcc aggccccggg gcccgtagat gtgccacgaa
     1621 ctgccgggct ggaacagggg ggcgaagaac ggcaagccct ggatgtggtc ccagtgggtg
     1681 tggccgatga ggaggtggcc ctccatcggc tcaccaccgt tgcgggcgac gatgtcctcg
     1741 ccgagaccat gggcgccggt gccgcagtcg aggaccacga tggtcccggc atcggagcgg
     1801 acttcgacac aggacgtgtt cccgccgaag cggagcgtgg agggtcccgc cttggcgatc
     1861 gatccacgtg ttccccagaa ggtcacctgc atgggacgcg cccctcgccc gaccgtagcc
     1921 aaacggggtg ccggcccgag catccaccgt ttggcggcct gcgggcggga gaattgtgct
     1981 ggtgcatgaa ccgggggtgc acaccggcgg gggcggctgg cgcagccttt ggcgcggccg
     2041 ggtcgacgtc gaccctcagc tccccgccct cctcgggcgg atcctcgacc gactgggccg
     2101 ctacctcgac gagggtggcc tcgacgcccc gacctgggtc accgacatgg ccgcgctcga
     2161 gcgccagctc acctacctgg cggggggcac ccgggaccgg gcgctgcact ccctc
//
LOCUS       DASTFV010000245         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_936444, whole genome shotgun sequence.
ACCESSION   DASTFV010000245 DASTFV010000000
VERSION     DASTFV010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936444"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1489)
                     /locus_tag="VFP02_04650"
     CDS             complement(200..1489)
                     /locus_tag="VFP02_04650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9542344.1"
                     /translation="MNPPGWLVDRLPEWARSIRFRYTLLYSAVLFGLAAVVVAAIYLV
                     LVMSLRGEPVSPTYTKKWCSNVSNDCVTINYFQAREFARFVHSVTLTKLRNYSFGALG
                     VLFVASLGVGWVVAGRVLRPIGRITAVARDIQATDLSRRIELPGPDDELKQLADTFDA
                     MLARLDAAFAAQRQFIADASHELRNPLAIIRTNVDVALADPNADPEDLRQTIAVVKRA
                     SGRMARLVDDLLALARRQEPILEHEPVDLGVAVAEASDDFVVPAAARNIVLDRVTAPD
                     VVVTGDRDALKRAVANLLENAVRLAPEGSRIRLATGSEGDRAWIAVTDEGPGIAPEDQ
                     PHVFDRFWRADKGRSRADGGTGLGLAIVRQIAESHGGEVRLQSKVGVGSSVVIWLPVT
                     PTPSDPTPRAPTPPVTTPVTTPADRARTRPSFGPPSE"
     gene            complement(1486..2166)
                     /locus_tag="VFP02_04655"
     CDS             complement(1486..2166)
                     /locus_tag="VFP02_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9542345.1"
                     /translation="MRVLVVEDEADLADAIARGLRREGYAVDVAYDGDEAVDKASVND
                     YDVVCLDLNLPRLDGIEVVRRIRADDVGQPTRVLMLTARDSTHDRITGLDEGADDYLV
                     KPFDFDELKARVRALLRRDAGRSGAVLRVGDLELDSSRHDARRDGHPLPLTPKEFALL
                     RYFMSRPGQVLSQEELLEHVWDENADPFTNTVRVTVMTLRRKLGDAGDSQPIETVVGR
                     GYRLREQP"
BASE COUNT          336 a          745 c          908 g          330 t
ORIGIN      
        1 ggggatgggt tgggtgtggg tgtcggagtc cggggagcgc gatggctcca agccgtcgcg
       61 aatggacatc ggtggcctcc tgctgggtcg aacatgacga accttccccg aggctaggcg
      121 ccgggcgcta aagcaccagt aaggcgaccc tcaaggcgag ataaggaccg ggccgggtcc
      181 aggcggcggg gcccaccccc tactccgacg ggggaccgaa cgagggccgg gtgcgggctc
      241 ggtcggctgg ggtggtgacg ggggtggtca ccgggggggt gggggcgcgg ggggtggggt
      301 cggagggggt gggggtgacg gggagccaga tgacgaccga ggagccgacc ccgaccttgg
      361 actggaggcg gacctcgccg ccgtggctct cggcgatctg gcggacgatg gccaggccca
      421 ggccggtgcc gccgtcggcg cgggaacggc ccttgtcggc gcgccagaag cggtcgaaga
      481 cgtggggctg gtcttcgggg gcgatgccgg gaccctcgtc ggtgaccgcg atccaggcgc
      541 ggtcgccttc gctgccggtg gccaggcgga tgcgggaacc ctcgggggcg aggcggacgg
      601 cgttctcgag caggttggcc acggcgcgct tgagggcgtc acggtcgccg gtgacgacca
      661 cgtcgggggc ggtgacccgg tcgaggacga tgttgcgggc ggcggccggg accacgaagt
      721 cgtcgcttgc ctcggcgacg gccacgccca ggtcgaccgg ctcgtgctcc aggatcggct
      781 cttggcgcct ggccagggcg agcaggtcgt cgaccaggcg ggccatgcgg ccgctggccc
      841 gtttgaccac ggcgatggtc tggcggaggt cctcggggtc ggcgttcggg tcggccaggg
      901 cgacgtcgac gttggtgcgg atgatggcca gcgggttgcg cagctcgtgg gaggcgtcgg
      961 cgatgaactg gcgctgggcg gcgaacgcgg cgtccaggcg ggccagcatg gcgtcgaagg
     1021 tgtcggccag ctgcttcagc tcgtcgtcgg ggccgggcag ctcgatgcgg cgcgacaggt
     1081 cggtggcctg gatgtcgcgg gccaccgcgg tgatgcggcc gatgggacgc agcacccggc
     1141 cggcgaccac ccagccgacg ccaaggctgg ccacgaacag caccccgagg gcgccgaagg
     1201 agtagttgcg aagcttggta agggtcaccg agtgcacgaa gcgcgcgaat tccctggcct
     1261 ggaagtagtt gatggtcacg cagtcgttgc tgacgttgct gcaccacttc ttggtgtagg
     1321 tcggcgacac cggctcaccg cgcagcgaca tgaccagcac caggtagatg gccgccacca
     1381 ccaccgccgc cagcccgaac aacaccgccg agtacagcag ggtgtagcgg aagcggatcg
     1441 acctggccca ttccggcagc cggtcgacca gccagccggg cgggttcacg gctgctcgcg
     1501 caggcggtag ccgcgaccga cgacggtctc gatcggctgg ctgtcgccag cgtcgcccag
     1561 cttgcggcgc agcgtcatca cggtcacccg gacggtgttg gtgaaggggt cggcgttctc
     1621 gtcccagacg tgctccagca gctcctcctg gctgagcacc tgcccgggcc tggacatgaa
     1681 gtagcgcagg agcgcgaact ccttgggcgt caggggcagc gggtgaccgt cgcggcgggc
     1741 gtcgtgccgt gagctgtcca gctcgaggtc gccgacccgc agcacggccc cggagcggcc
     1801 ggcgtcgcgg cgcagcaggg cccgcaccct ggccttgagc tcgtcgaagt cgaacggctt
     1861 gaccaggtag tcgtcggcgc cctcgtccag gccggtgatc cggtcgtggg tcgagtcgcg
     1921 ggcggtgagc atcagcaccc tggtcggctg gccgacgtcg tcggcccgga tgcgccgcac
     1981 cacctcgatg ccgtcgagcc ggggcaggtt caggtcgagg cagaccacgt cgtagtcgtt
     2041 gacgctcgcc ttgtcgacgg cctcgtcacc gtcgtaggcg acgtccacgg cgtagccctc
     2101 ccgacgaagg ccgcgcgcga tcgcgtcggc caggtcggcc tcgtcctcga cgaccagcac
     2161 ccgcatgccc tgaatggtag ctgtcgacgc catcaagcag gcgtaagagg ccagcttgtt
     2221 agactgcgac atggcctccg acaccaaccg ggccggagcc ggcgtcctcg tggccatcgg
     2281 tggtgcggag gacaagctcg gccaccggac catcctcaa
//
LOCUS       DASTFV010000246         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_944925, whole genome shotgun sequence.
ACCESSION   DASTFV010000246 DASTFV010000000
VERSION     DASTFV010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944925"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFP02_04660"
     CDS             <1..325
                     /locus_tag="VFP02_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET9542346.1"
                     /translation="EMRDELTRVMKSKTRDEWCALMEASDVCFAPVLSMEEAPSHPHN
                     VARDTFVDVAGVPQPNAAPRFSRTPGAIQGPPAHAGQHTDEILAEAGLSADEIAALRD
                     AGAVA"
     gene            325..1125
                     /locus_tag="VFP02_04665"
     CDS             325..1125
                     /locus_tag="VFP02_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET9542347.1"
                     /translation="MASLVVLHAHPDDESIQTGGTMAKAAAEGHRVTLVVATRGEHGE
                     VVPGVLDEGEPLGLRRITETYRSAELLGVQRVEFLGYVDSGMMGEPTNDERACFWQAD
                     VDHAARRLALILEEEQAEVLTYYDEIGGYGHPDHIKVHEVGRRAGELAGTPHRFMATM
                     NRDFILELVRARMESGEFDLGDAEAPEIDEENFGQPASRITHCIDVSAQIEAKRASMR
                     AHASQIPDDHFFLALPDEAFAFSFGQEWFIDDQDGATGGEPFASDLFG"
     gene            1144..1710
                     /locus_tag="VFP02_04670"
     CDS             1144..1710
                     /locus_tag="VFP02_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9542348.1"
                     /translation="MSNPRWRGINHLALITPDMDATVRFYHGALGMPIVATINAGPMR
                     HYFFDMGMGNTIAFFCWSDAEVDTFEKPAGIPPDFPAQFDHVSFNLPELRDLIDLQGR
                     LREHGVEVTELVDHGFVQSVYFTDPNGIALEASYWVTEPTGRPADYVDGEMFGDTDPV
                     PAVRELMATGELASIPSTRLVDEAADPA"
     gene            complement(1774..>2028)
                     /locus_tag="VFP02_04675"
     CDS             complement(1774..>2028)
                     /locus_tag="VFP02_04675"
                     /inference="COORDINATES: protein motif:HMM:NF018287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HET9542349.1"
                     /translation="GFVDPGFGYDPTGLLRGSRAALEVRAHDVPFMVEHRQPVCKLTF
                     ERMLEPPERLYGAGIGSNYQGQVETLGKHFIRPTAHPSLR"
BASE COUNT          319 a          724 c          688 g          297 t
ORIGIN      
        1 ggagatgcgc gatgagttga cccgggtcat gaagtcgaag acccgtgatg aatggtgcgc
       61 cctgatggaa gccagtgacg tgtgcttcgc cccggtcctg tccatggagg aggctccgag
      121 ccacccccac aacgtggccc gcgacacgtt cgtggacgtg gccggcgtgc cccagccgaa
      181 cgccgccccc cgcttcagcc ggacccccgg cgccattcag ggccccccgg cgcatgccgg
      241 ccagcacacc gacgagatcc tggccgaggc cggactgagc gctgacgaga tcgccgccct
      301 gcgggatgcg ggagcggtgg cctagtggcc tcactggtcg tcctccacgc ccatcccgac
      361 gacgagtcga tccagaccgg cggcaccatg gccaaggcgg cggctgaggg tcaccgggtg
      421 acgttggtcg tggccacgcg gggcgagcac ggcgaagtcg tgcccggtgt cctcgacgag
      481 ggtgagcccc tcggcctccg ccgcatcacc gagacctacc ggtccgccga gctgctcggt
      541 gtccagcggg tcgagttctt gggctatgtc gactccggga tgatgggcga gcccaccaac
      601 gacgagcggg cctgcttctg gcaagccgac gtcgatcacg ccgcccggcg cctggccctc
      661 atcctcgagg aggagcaggc cgaggtcctc acctactacg acgagatcgg cggctacggc
      721 caccccgacc acatcaaggt ccacgaggtc ggtcggcggg cgggggagct ggccgggaca
      781 ccgcaccgct tcatggccac catgaaccgg gacttcatcc tcgagctggt ccgagcccgg
      841 atggagtccg gcgagttcga cctcggtgac gccgaggcgc ccgagatcga cgaggagaac
      901 ttcggccagc ccgcgtcccg gatcacccac tgcatcgacg tctccgccca gatcgaggcg
      961 aagcgggcct cgatgcgggc ccacgccagc cagatccccg acgaccactt cttcctggcc
     1021 ctgcccgacg aggccttcgc cttcagcttc ggccaggagt ggttcatcga cgatcaggac
     1081 ggcgcgactg gcggcgagcc gttcgcgagt gacctgttcg gctgacgggc ttacgctcgg
     1141 ggcatgagca acccccgctg gcgaggcatc aaccacctgg cgctcatcac gcccgacatg
     1201 gacgcgacgg tgcgcttcta ccacggggcc ctgggcatgc cgatcgtggc cacgatcaat
     1261 gccgggccca tgcggcacta cttcttcgac atggggatgg gcaacaccat cgccttcttc
     1321 tgctggtccg acgccgaggt cgacaccttc gagaagccgg cgggcatccc gccggacttc
     1381 ccggcccagt tcgaccacgt gtcgttcaac ctgcccgagc tgcgggacct gatcgacctg
     1441 cagggccgcc tccgggagca cggcgtcgag gtgaccgagc tggtcgacca cggcttcgtc
     1501 cagtccgtct acttcaccga ccccaacggc atcgccctcg aggccagcta ctgggtgacc
     1561 gagcccaccg gccgcccggc ggactacgtc gacggtgaga tgttcggcga caccgacccc
     1621 gtccccgccg tccgcgagct catggccacc ggcgagctgg ccagcatccc gtccacccgc
     1681 ctggtcgacg aggccgccga cccggcctga ttgcccggtt ctggttgcac ccagccgcgt
     1741 cgggtcgcgg ggacgcgcaa ccagaacggt gggtcagcgc agtgaagggt gggcggtggg
     1801 gcggatgaag tgcttgccca gggtctcgac ctggccctgg tagttggagc cgataccggc
     1861 gccgtacagg cgctcgggcg gctccagcat ccgctcgaac gtcagcttgc agacgggttg
     1921 gcggtgctcg accatgaacg ggacgtcgtg ggcgcggact tcgagggccg ctcgtgaccc
     1981 ccgcagcagg ccggtggggt cgtagccgaa gcccgggtcg acgaagcc
//
LOCUS       DASTFV010000247         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_944981, whole genome shotgun sequence.
ACCESSION   DASTFV010000247 DASTFV010000000
VERSION     DASTFV010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944981"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..555)
                     /locus_tag="VFP02_04680"
     CDS             complement(13..555)
                     /locus_tag="VFP02_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542350.1"
                     /translation="MTVDLTARIVALHEAFDRAALPHAFGGALALAWCTQQARGTIDI
                     DVNLFVDAGRAPEIFAALPTEVTWTEEDVAQVERDGQVRCWWETTPVDLFANTTAFHA
                     QAAARVRRELFAGTEVPFLDCGDLAVFKAFFDRTRDWADLEAMLEAGTLDVEAVVGVL
                     ARHLGADDHRIERLLSLTSA"
     gene            complement(552..872)
                     /locus_tag="VFP02_04685"
     CDS             complement(552..872)
                     /locus_tag="VFP02_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9542351.1"
                     /translation="MGAATTVRQARRRAGMSLRELAARAGTSHATLSAYENGAKTPTV
                     KTLERIVRAAGFALDLELVPRVDGDEPTDRGQELEAVLALAAQFPARHAATLPFPPFP
                     EAPT"
     gene            964..>2194
                     /locus_tag="VFP02_04690"
     CDS             964..>2194
                     /locus_tag="VFP02_04690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HET9542352.1"
                     /translation="MTTAERARMLLAEFLGPDAEFRDGQLDAIEALVDGRHRVLVVQR
                     TGWGKSIVYLIATRLLREDGAGPSLVVSPLLALMRNQTLMGERIGVRSATINSENIDD
                     WQAIEAAAHAGDIDLLLISPERLNNPRFRDNVLAEVAHTAGLFVVDEVHCISDWGHDF
                     RPDYRRVGRVIDLLPPTVPVLGCTATANDRVVADIEHQLGDDLVVIRGPLGRDSLAVR
                     VLDLPTQGERLAWLSREVPRMDGTGVVYCLTIADANRVTQWLRREGIDAVSYTGATAP
                     EDRLEIEAALSENRVKVVVATSALGMGYDKPDLAFVVHFQSPGSPIAYYQQIGRAGRA
                     LDTAAVVLLRGHEDRDIQDWFIETAFPDKVDAEAVVAFLESQEEPVGVAAIEPVVNLR
                     RSRLDAMLKVLEVEGAVA"
BASE COUNT          303 a          799 c          792 g          300 t
ORIGIN      
        1 atcgccgagc gctcaggctg aggtcaggct cagcaaccgc tcgatgcggt ggtcgtcggc
       61 tccgaggtgc cgagccagca cgcccacgac ggcttcgacg tccagcgtgc ccgcctcgag
      121 catcgcctcg aggtcggccc agtccctcgt gcggtcgaag aaggccttga acaccgcgag
      181 gtccccacag tcgaggaagg ggacctccgt gcccgcgaag agctcgcgtc ggacccgggc
      241 cgcggcctgc gcgtggaacg cggtcgtgtt ggcgaagagg tcgaccggcg tggtctccca
      301 ccagcagcgg acctggccgt cccgctcgac ctgggccacg tcctcctcgg tccaggtcac
      361 ctcggtcggg agggcggcga agatctccgg ggcccggccg gcgtcgacga acaggttgac
      421 atcgatgtcg atcgtgcccc gggcctgctg tgtgcaccag gccagggcca gggcgccgcc
      481 gaaggcgtgg gggagggcgg cccggtcgaa ggcctcgtgc agggcgacga tccgggccgt
      541 cagatcgacc gtcacgtcgg tgcctcggga aacgggggga aggggagggt ggcggcgtgc
      601 cgggcgggga actgggccgc cagggcgagc acggcctcga gctcctggcc ccggtcggtg
      661 ggctcgtcgc cgtcgacccg ggggacgagc tcgaggtcga gggcgaagcc cgcggcccgc
      721 acgatgcgct cgagggtctt gacggtcggg gtcttcgccc cgttctcgta ggccgagagc
      781 gtggcgtggg acgtgcccgc ccgggccgcc agctcccgca ggctcatccc cgcccgtcgc
      841 cgggcctgcc ggaccgtcgt ggccgcaccc atgacccgat ggtatctgat cagataccac
      901 ttcgtcacgg gcgccgccgg cgcccgccga cgagtgtcac cccccggccg tacggtgccg
      961 ggcgtgacca ctgcggaacg ggcccggatg ctgctggccg agttcctcgg gcccgacgcc
     1021 gagttccggg acggtcagct cgacgccatc gaggccctgg tcgacggccg ccaccgggtc
     1081 ctggtcgtcc agcgcacggg ttggggcaag agcatcgtct acctgatcgc cacccgcctg
     1141 ctgcgagagg acggcgccgg gcccagcctg gtcgtctcgc cgctgctggc cctcatgcgc
     1201 aaccagacgt tgatgggcga gcgcatcggc gtccgctcgg ccaccatcaa cagcgagaac
     1261 atcgacgact ggcaggccat cgaggccgcc gcccacgccg gcgacatcga cctgctcctc
     1321 atctcccccg agcgcttgaa caacccccgc ttccgggaca acgtcctggc cgaggtggcc
     1381 cacacggccg ggctgttcgt ggtcgacgag gtccactgca tctcggactg gggccacgac
     1441 ttccggcccg actaccgccg ggtcggccgg gtcatcgacc tgctgccccc gacggttccc
     1501 gtcctgggct gcacggccac ggccaacgac cgcgtggtgg ccgacatcga gcaccagctg
     1561 ggcgacgacc tcgtcgtcat ccggggcccg ctggggcggg acagcctggc cgtgcgggtc
     1621 ctcgacctgc ccacccaggg cgagcgcctg gcctggctga gccgggaggt gccccgcatg
     1681 gacggcaccg gcgtcgtcta ctgcctgacc atcgccgacg ccaaccgggt cacccagtgg
     1741 ctgcggcggg agggcatcga cgccgtctcc tacacggggg ccacggcccc ggaggaccgg
     1801 ctggagatcg aggccgcgct cagcgagaac cgggtcaagg tcgtggtggc cacctccgcc
     1861 ctgggcatgg gctacgacaa gcccgacctc gccttcgtcg tccacttcca gtcaccgggc
     1921 tcgcccatcg cctactacca gcagatcggg cgggcggggc gagccctgga caccgccgcc
     1981 gtcgtgctgc tgcggggcca cgaggaccgg gacatccagg actggttcat cgagacggcc
     2041 ttcccggaca aggtcgacgc cgaggccgtc gtcgccttcc tggagagtca ggaggagccg
     2101 gtcggcgtgg ccgccatcga gccggtcgtc aacctgcgcc gctcccgcct ggacgccatg
     2161 ttgaaggtgc tcgaggtcga gggcgcggtg gccc
//
LOCUS       DASTFV010000248         3702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_947009, whole genome shotgun sequence.
ACCESSION   DASTFV010000248 DASTFV010000000
VERSION     DASTFV010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947009"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VFP02_04695"
     CDS             <1..511
                     /locus_tag="VFP02_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542353.1"
                     /translation="KGSTWEGGMRVPGIAVWPGMIDADRRSDGLFCFTDLLPTVLSLA
                     GEEDRIPDDRFIDGVDQASFLLGEDAVSNRKYVWYWLLSTLSAVRCGEYKFMIASTSD
                     DDTDTWGPGGFTGVTQNYTYARTFNLYLDPKEQHSYMTRKIAYNEAFLTGIGQHLRSY
                     RDFPAKKII"
     gene            complement(515..994)
                     /locus_tag="VFP02_04700"
     CDS             complement(515..994)
                     /locus_tag="VFP02_04700"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HET9542354.1"
                     /translation="MSDDDRRRDQGEESEEQRERYRELLEETRTVMPGTQVLFAFLLT
                     AAFSTGFKDLDLLGRRSFAGALLLAALAAIILMGPTAFHRVSEPDSRAERLVVSVYLQ
                     VIGMALLLASMTLVMFVVTRLIFDDTAVGLAFAAVAGGAGVLVWYVLPRVSHPDPAD"
     gene            1091..1363
                     /locus_tag="VFP02_04705"
     CDS             1091..1363
                     /locus_tag="VFP02_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542355.1"
                     /translation="MASRNAKRKRQQQQQARHSADKSHERDRTQSHKPLPKVGTRPEQ
                     EHLHRQRQADLVDFGRGGSHRPLGIALVVLALVLLLALLAVLVLFD"
     gene            complement(1368..3062)
                     /locus_tag="VFP02_04710"
     CDS             complement(1368..3062)
                     /locus_tag="VFP02_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET9542356.1"
                     /translation="MTIRPLIRGVLMAPASRSTLIARDISVSFGPVTVLDAVSLTVGP
                     GDRLGLVAPNGTGKSTLLRALAGLLEPDEGRVDLAPPSATIGLLAQEPERRTDETVRQ
                     AVARRTGVAEAAEEYEAATIAVATAPDAAADRYSVALDRWLALGGADLDARIGETWSE
                     LGLPDRLLDQPTATLSGGEAARASLAAVLLSRHDVLLLDEPTNDLDFAALDHLERFVL
                     RREAPLLLVSHDRAFLERTITGVIELDEHSRRATRYEGGWLAYLEEQAVARRHAEEAY
                     ELYESTKGELVARAHRQRQWSDQGVIHRNKNPKDNDKFLRNFLNDRTEKQAGKVRQSE
                     KAILRLEANAPDKPRTSWALRFEIAEATRSGQVVASLDGAVVDRGAFRLGPVDLEVGW
                     ADRVAIVGPNGAGKSTLLDALLGRIELAAGRQRLGPGVVVGEIDQGRDLLDGDGPLLD
                     TFQEAAGMASTGEARTLLAKFGLGAAHVLRPAHTLSPGERTRAGLALLQGRGVNCLVL
                     DEPTNHLDLPALEQVEQALATYAGTVLLVSHDRRLLEAVTTDRTLHIDAGQVTEVG"
     gene            complement(3231..>3702)
                     /locus_tag="VFP02_04715"
     CDS             complement(3231..>3702)
                     /locus_tag="VFP02_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542357.1"
                     /translation="LGMLHQALRDLSAWVETGVAPPPSTSYEVVDGQVVVPDDARERR
                     GLQPVVSLTADGGARTDVPVGAEVTFRATAAVPDGTGAMVTVEWDVDGAAEFPVREDV
                     VAGERAVVEQRHSYAEPGTYFPTVRVAAHRDGDGTTPYARIQNLARVRVVVTTP"
BASE COUNT          559 a         1295 c         1309 g          539 t
ORIGIN      
        1 caagggctcg acctgggagg ggggcatgcg ggtgccgggc atcgcggtct ggccggggat
       61 gatcgacgcc gaccgccgga gcgacggcct gttctgcttc accgacctgc tccccaccgt
      121 gctcagcctg gcgggggagg aggatcgcat ccccgacgac cgcttcatcg acggcgtgga
      181 ccaggcctcg ttcctgctgg gagaggacgc cgtgtccaat cggaagtacg tctggtactg
      241 gctgctgtcc acgctctccg ccgtgcgttg cggcgagtac aagttcatga tcgcctcgac
      301 cagcgatgac gacaccgaca cctggggacc gggcggcttc accggcgtga cccagaacta
      361 cacctacgct cgcacgttca acctctacct ggacccgaag gagcagcaca gctacatgac
      421 ccgcaagatc gcctacaacg aggccttcct gacgggcatc ggccagcacc tccgcagcta
      481 tcgggacttc cccgccaaga agatcatctg acggtcagtc cgccgggtcg gggtggctga
      541 ccctggggag gacgtaccag accagcacgc ccgcgccgcc ggccacggcg gcgaaagcga
      601 gcccgacggc ggtgtcgtcg aagatcagcc gggtgacgac gaacatgacc agggtcatgg
      661 aggcgaggag gagggccata ccgatgacct ggaggtacac gctgacgacc aggcgctcgg
      721 cccgactgtc gggctcggag acacggtgga acgccgtggg tcccatgagg atgatggccg
      781 cgagcgccgc caggagcagg gccccggcga acgagcggcg gccgagcagg tcgaggtcct
      841 tgaacccggt gctgaacgcg gcggtcagca ggaaggcgaa cagcacctgg gtgccgggca
      901 tgaccgttcg cgtctcctcc aacagctccc gatagcgctc gcgctgctcc tcgctctcct
      961 cgccctggtc gcgtcgccgg tcgtcgtcgc tcatggtcgt cctttgtcgg tgacgacggc
     1021 aattaccccg gagagcgggc cgccattccc ccgatcggga ggggcgcgca cccccgagta
     1081 cggtgctccg atggcctcga gaaacgccaa gcgcaagcgg caacagcagc agcaagcccg
     1141 gcacagcgcg gacaagagcc atgagcgcga ccgcacgcag tcccacaagc ccctgcccaa
     1201 ggtgggtacc cgccccgaac aggagcacct ccatcgccaa cgacaggccg acctggtcga
     1261 cttcgggcgg ggcggcagtc accgaccgtt gggcatcgcc ctggtggtcc tcgctctggt
     1321 cctgctcctc gcccttctcg cggtcctcgt cctgttcgac tgactggtca cccgacctcg
     1381 gtgacctggc cggcgtcgat gtggagcgtg cggtcggtcg tgaccgcctc cagtagccgc
     1441 cggtcgtggc tgaccagcag gactgtgccc gcataggtgg ccagcgcctg ctcgacctgc
     1501 tcgagggcgg gaaggtcgag gtggttggtg ggctcgtcga gcaccaggca gttcacaccc
     1561 cgaccctgca gcagggccag gccggctcga gtccgctcgc ccggtgacag cgtgtgggcc
     1621 ggtcggagga catgggccgc gccgaggccg aacttggcca gcagggtccg ggcctccccc
     1681 gtggaggcca tcccggcggc ctcctggaac gtgtcgagca ggggaccatc accatcgagg
     1741 aggtcacgtc cctggtcgat ctcaccgacc acgacaccgg gcccgaggcg ctgtcgcccg
     1801 gcggccagct cgatccggcc gaggagggcg tcgagcagcg tcgacttccc cgcgccgttg
     1861 ggtccgacga tggcgacccg gtcggcccag ccgacctcca ggtcgaccgg accgaggcgg
     1921 aaggcccccc gatcgaccac ggcgccgtcc agggaggcca cgacctgacc actgcgggtg
     1981 gcctccgcga tctcgaaccg cagcgcccag ctcgtgcggg gcttgtccgg ggcgttggcc
     2041 tccaggcgga ggatcgcctt ctcggactgg cgcaccttgc cggcctgctt ctcggtccgg
     2101 tcgttgagga agttccgtag gaacttgtcg ttgtccttcg ggttcttgtt ccggtggatc
     2161 acgccctggt cggaccactg gcgctgtcgg tgggctcggg ccaccagctc gcccttggtc
     2221 gactcgtaga gctcataggc ctcttccgcg tggcggcggg cgacggcctg ttcttcgagg
     2281 taggccagcc agccgccctc gtagcgggtg gcccggcggg agtgctcgtc gagctcgatc
     2341 acccccgtga tggtgcgttc gaggaacgcc cggtcgtggc tcacgagcag gaggggggcc
     2401 tcccgccgga ggacgaagcg ctccaggtgg tccagggcgg cgaagtccag gtcattggtg
     2461 ggttcgtcga ggagcaacac gtcatggcgg gacaggagca cggcggccag cgacgccctc
     2521 gccgcctcgc cgccggacag cgtggccgtg ggctggtcga gcaggcggtc gggcaggccg
     2581 agctcagacc acgtctcgcc gatgcgggcg tcgaggtcgg ccccgcccag ggccagccac
     2641 cggtccagtg cgaccgagta ccggtcggcc gcagcgtcgg gcgccgtggc gacggcaatg
     2701 gtggccgcct cgtactcctc ggccgcctcg gcgacgccgg tgcggcgggc gacggcctga
     2761 cgcacggtct cgtcggtccg gcgttcgggc tcctgggcga gcaggccgat cgtggccgag
     2821 ggtggcgcca gatcaacgcg accttcgtcg ggctcgagca accccgccag ggcccgcaag
     2881 agggtcgact tgccggtgcc gttgggggcg accagcccca gtcggtcacc cggcccgacc
     2941 gtgagcgaga cggcgtcgag caccgtcacc gggccgaagg agaccgagat gtcgcgggcg
     3001 atgagggtgg aacgggaggc gggggccatg agcactcctc ggatgagcgg acggatggtc
     3061 acggatccga gttctcagcg cacggtccca cggtaggcgt cgccttcggc ccgccagcgc
     3121 cgatttcccc ggacggctcg cgagggtggg ctcgattcgc cgatgggacc gatggtcttt
     3181 cgtggggcac tctgctcgga gcgggcagca atcgttcgag atcgccccgg ctacggggtc
     3241 gtgacgacga cccggacccg ggccaggttc tggatccggg cgtaaggcgt cgtcccgtct
     3301 ccgtcgcggt gggccgccac tcggacggtc gggaagtagg tgccgggttc ggcatacgag
     3361 tgtcgctgct cgacgacggc tcgctcaccc gccaccacgt cctctcggac cgggaattca
     3421 gcagcaccgt cgacgtccca ctcgacggtc accatcgcac ccgtcccgtc cggtacggcc
     3481 gccgtcgccc ggaaggtcac ctcagcaccg accggcacgt cggtccgagc cccaccgtcg
     3541 gcggtcagcg acaccaccgg ctgcaacccg cgccgctcgc gggcgtcgtc gggcacgacg
     3601 acctggccat ccacgacctc atagctcgtg ctcggtggcg gcgcgacccc cgtctcaacc
     3661 caggcactga ggtcacggag cgcctgatgc agcatcccca gg
//
LOCUS       DASTFV010000249         3536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_955370, whole genome shotgun sequence.
ACCESSION   DASTFV010000249 DASTFV010000000
VERSION     DASTFV010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955370"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VFP02_04720"
     CDS             complement(<1..946)
                     /locus_tag="VFP02_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HET9542358.1"
                     /translation="MRAIVLVGGFGTRLQPLTLATPKQMLPVVDRPMIEWVVGHLGEH
                     GVDEAILALGYRPDAFMAAYPDAECAGVRLRYAVEDSPLDTAGAIRFAASEAGVDERF
                     LVVNGDVLTDLDIGALVAFHDAAGGAGTIHLHPVDDPSRYGVVPIDADGRVVEFVEKP
                     SRDQAPSRWINAGAYVLEPDVLNRIDPEGKVSIERVTFPALAAEGRLFGFQTETYWID
                     AGTPATYLRAQLDLVDGHRATTPPAVAADATVADGAKVEHAVLMAGASVADGASVVDS
                     AVLPGARVERGATVRDAVVGPDAVVGTDAVVDGGTVLGA"
     gene            complement(943..1608)
                     /locus_tag="VFP02_04725"
     CDS             complement(943..1608)
                     /locus_tag="VFP02_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542359.1"
                     /translation="MVCVLGVSLILYARTQDEAQALEPGVTDHWHAAYAIDICGTIQP
                     PLTNDADPNGTHTHGDGVMHIHPGAAQQVAKAGEDATIGVFMSAAGATLTDDSISFPD
                     ETGEIVTYTEGEDQCDGEDGQVQVAYWANASEANDTDPQVFTDNLADIFFKNDREAYT
                     IAFVPEGDDILPPTTVADLDELAGIDSGALDPSTTSTTTVAPGGRPATTTSAPTTTAA
                     PAG"
     gene            complement(1753..2985)
                     /locus_tag="VFP02_04730"
     CDS             complement(1753..2985)
                     /locus_tag="VFP02_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9542360.1"
                     /translation="MNLTVIAPHFTPDVAPTGEVMTSIVEELALRGHRLHVVTALPWY
                     RHHRIEHGWHGRLVRTEATDWGHISRVHPFPTDKTNIPARALAFGGFTVLAGLVGLLH
                     RQRPDAVLAMSPPLTLGLAGWSVARARRAPFVFNIQDVFPDVAVEAGVITGRRAIAGA
                     RALERFTYRRSDAVTVLSDDLADNLRAKLRPGERERIRVIPNFIDTGRVVPGDHQNDY
                     RAEFGLGERRVVMYAGNLGYSQPVGLVLEAARRLAGREDVVFVVNGAGSAREALRAEA
                     DDLPNVIFVDLQPRERLAEVLAAGDIHLVLLRTGFARSSVPSKLYSILAASRPLVASV
                     DPGTEVARVVDEAGAGTAVAPDDPDALVGALLPLIDDPERCQKLGDAGRRFVETWPSP
                     GDVAQRYEALFTELRVAR"
     gene            complement(2982..>3536)
                     /locus_tag="VFP02_04735"
     CDS             complement(2982..>3536)
                     /locus_tag="VFP02_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET9542361.1"
                     /translation="FGIKAPFFVYPAITYPHKNHLTLVRAFADVVREHPKASLVLTGG
                     EGQMEGEILETISALGLDGRVHRLGRIPWWDLAAILRQARGLGFPSRFEGFGAPVIEA
                     MARGCPVVAADATALPEVVGDAGVLVEADDQKAWATELLRLLDDDEHHARLVAAGRAR
                     AAQFSAVASAEALLAAYREVLSGS"
BASE COUNT          512 a         1209 c         1338 g          477 t
ORIGIN      
        1 cggcgccgag cacggtcccg ccgtcgacca ccgcatcggt gcccacgacg gcatcggggc
       61 cgacgacggc gtcgcggacg gttgccccgc gctccacccg ggcaccaggg aggaccgcac
      121 tgtcgaccac actggccccg tcggccacgg aggcaccggc catgaggacg gcgtgctcga
      181 ccttggcccc gtcggccacg gtggcatcgg cggccacggc gggcggggtg gtggcccgat
      241 ggccgtcgac caggtcgagc tgggcccgga gatacgtggc tggagtgccg gcgtcgatcc
      301 agtaggtctc ggtctggaag ccgaacagcc gaccttcggc cgccagggcc gggaacgtca
      361 cccgctcgat ggacaccttc ccctcgggat cgatccggtt cagcacgtcg ggctcgagca
      421 cgtaggcccc ggcgttgatc cagcgcgacg gggcctgatc ccgggacggc ttctcgacga
      481 actcgacgac ccggccatcg gcgtcgatgg gcaccacgcc gtaccgggag gggtcgtcga
      541 cggggtggag atggatcgtt cccgccccac cggcggcgtc gtggaaggcg acaagggcgc
      601 cgatgtccag gtcggtgagg acgtcaccgt tgaccacgag gaagcgctcg tcgacgccgg
      661 cctcggaggc ggcgaagcgg atggcgccgg cggtgtcgag gggcgagtcc tccacggcgt
      721 agcgcaggcg caccccggca cactcggcgt cgggataggc ggccatgaag gcgtcgggcc
      781 ggtagccgag ggccaggatc gcctcgtcca cgccatgctc gcccaggtgg ccgacgaccc
      841 attcgatcat gggccggtcc accaccggga gcatctgctt cggcgtggcc agggtcagcg
      901 gctggaggcg ggtaccgaag cctccgacca ggacgatggc cctcatcccg cgggcgccgc
      961 cgtcgtggtg ggagccgagg tggtggtcgc cggacgaccg ccgggggcga ccgtggtcgt
     1021 gctcgtcgtg gacgggtcga gcgcaccgga gtcgatgccg gccagctcgt cgaggtcggc
     1081 gacggtggtc ggcggcagga tgtcgtcgcc ctcggggacg aaggcgatcg tgtaggcctc
     1141 gcggtcgttc ttgaagaaga tgtcggccag gttgtcggtg aacacctgcg ggtccgtgtc
     1201 gttggcctca ctggcgttgg cccagtaggc gacctggacc tgaccgtcct caccgtcgca
     1261 ctggtcctcg ccctcggtgt aggtgacgat ctcgccggtc tcgtcgggga agctgatcga
     1321 gtcgtcggtc agcgtggccc cggcggcgct catgaagacg ccgatggtgg cgtcctcacc
     1381 ggccttggcc acctgctgtg cggcaccggg gtggatgtgc atgacgccgt cgccgtgggt
     1441 gtgggtcccg ttgggatcgg cgtcgttggt caacgggggc tggatggtgc cgcagatgtc
     1501 gatggcgtac gccgcgtgcc agtggtcggt gacccctggc tccagcgcct gggcctcgtc
     1561 ctgggtccgc gcgtagagga tgagggagac cccaaggacg cagaccaccg tgatggtggc
     1621 ggggaagagg aggctacgcc gctgtccgcc acgggaccca cggcccccgg cgcgcgccgc
     1681 acgagcgact ttcttggctg aagaggcctt acccatggcc cggagagggt atcgcccacc
     1741 ctggccgggg cgtcagcggg cgacccgcaa ctcggtgaac agcgcttcgt accgctgggc
     1801 gacgtcgccg ggagacggcc aggtctcgac gaagcgccgc ccggcatcgc ccagcttctg
     1861 gcaccgctcc ggatcgtcga tcaagggcaa cagcgcgccg accagggcgt cggggtcgtc
     1921 gggggccacg gccgtgcccg cccccgcctc gtcgaccacc cgcgccacct cggtgccggg
     1981 gtcgacgctg gccaccaacg gccgcgaggc ggcgaggatc gagtagagct tcgacggcac
     2041 gctggaccgg gcgaagccgg tccgcagcag gacgagatgg atgtcaccgg ccgccaacac
     2101 ctcggccagg cgctcccgag gctgcaggtc gacgaagatc acgttgggca ggtcgtcggc
     2161 ttcggcccgc agcgcctcac gggccgagcc ggcgccgttg acgacgaaga ccacgtcctc
     2221 ccgaccggcc agacggcgag ccgcttcgag gaccaggccc acgggctggg agtagcccag
     2281 gtttccggcg tacatcacga cccggcgctc ccccaggccg aactcggccc ggtagtcgtt
     2341 ctggtgatcc ccggggacga ctcggccggt gtcgatgaag ttggggatga cccggatgcg
     2401 ctcccgctcc cccggccgca gcttggcccg gaggttgtcg gccagatcgt cggagagcac
     2461 ggtgaccgcg tccgagcggc ggtaggtgaa ccgctccagt gcccgggccc cggcgatggc
     2521 ccggcggccg gtgatcacgc ccgcctcgac ggccacgtcg gggaagacgt cctggatgtt
     2581 gaagacgaac ggcgcccgtc gggcccgggc caccgaccag cccgccaggc ccagggtgag
     2641 gggtggggac atggccagca ccgcgtccgg gcgctgtcgg tgcaggagcc cgaccagccc
     2701 ggcgaggacg gtgaacccgc cgaaggccag ggcgcgggcg gggatgttcg tcttgtccgt
     2761 cggaaacggg tggacccggg agatgtggcc ccaatccgtc gcctcggtgc ggaccagccg
     2821 gccgtgccat ccgtgctcga tccggtgatg ccggtaccag ggcagggccg tgacgacgtg
     2881 gaggcggtgg ccccgcaggg ccagctcctc gacgatgctg gtcatgacct cgccggtcgg
     2941 ggcgacgtcg ggcgtgaagt ggggggcgat gacggtcagg ttcacgagcc ggacagcacc
     3001 tcgcggtagg cggccaggag cgcctcggcc gaggccaccg cgctgaactg agccgcccgg
     3061 gcccggccgg cggcgaccag tcgggcgtgg tgctcgtcgt cgtcgaggag acggagcagc
     3121 tcggtggccc acgccttctg gtcgtcggcc tcgacgagca caccggcatc gcccacgacc
     3181 tcgggcaggg cagtggcgtc ggccgctacg accggacagc cgcgagccat ggcctcgatg
     3241 accggcgcac cgaacccctc gaaccgggac ggaaagccca gaccgcgggc ctggcgcagg
     3301 atggcggcca ggtcccacca ggggatgcga ccgaggcggt ggacccggcc gtccagcccc
     3361 agcgcgctga tcgtctccag gatctcgccc tccatctgcc cctcgccccc ggtgagcacc
     3421 agagaggcct tggggtgctc ccggacgacg tcggcgaaag cccgcacgag cgtcaggtgg
     3481 ttcttgtggg ggtaggtgat cgccgggtag acgaagaacg gcgccttgat cccgaa
//
LOCUS       DASTFV010000250         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_956232, whole genome shotgun sequence.
ACCESSION   DASTFV010000250 DASTFV010000000
VERSION     DASTFV010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956232"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..253
                     /gene="rpmB"
                     /locus_tag="VFP02_04740"
     CDS             59..253
                     /gene="rpmB"
                     /locus_tag="VFP02_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET9542362.1"
                     /translation="MPAVCEICGKHPSFGMKLSHSHRRTRRRWNPNIQSVRAVVDGRT
                     RRVRACTSCIRAGKVVKAAR"
     gene            271..504
                     /locus_tag="VFP02_04745"
     CDS             271..504
                     /locus_tag="VFP02_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542363.1"
                     /translation="MQGVIKSYDPGTKVGVVVSDADLAEFDLSEEALTGSIFRTLRLG
                     QRVVFDLDGSGWASSVRMGSEVDLGTPDYLEER"
     gene            501..2312
                     /locus_tag="VFP02_04750"
     CDS             501..2312
                     /locus_tag="VFP02_04750"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HET9542364.1"
                     /translation="MSVSPTTNPPTSNRRLLEWVDEWAAIFQPDAIEWCDGSEEEYGR
                     MCRLLVEGGTFETLNPELRPNSFLGRSDPADVARVEDRTFICSEREIDAGPTNNWRPP
                     AEMKAEILEFFTGAMRGRTLYVVPFSMGPLGSRIAHIGVQLTDSPYVVANMRTMTRMG
                     TPVLKVLGAGSFVKCLHSVGYPLVDRDDVAWPCDGDNKYISHFPESREIWSYGSGYGG
                     NALLGKKCFALRIASAMAHDEGWLAEHMLILGVTPPDGDTKYIAAAFPSACGKTNMAM
                     MIPTLPGWKVECVGDDIAWMKFGDDGRLYAVNPEAGFFGVAPGTSEETNPNAMKTLGE
                     NIIFTNVAKTDDGDVWWEGMSPAPDHLVDWKGEDWSPGSDGPAAHPNARFTAPASQCP
                     AISPNWDSPAGVPISAILFGGRRASAVPLVTEAKDWEHGVFLGSIMSSEKTAAAAGTL
                     GEVRFDPFAMLPFCGYNVGEYFSHWLSIGEHATHAPNLPRLYWVNWFQKDEDGKFLWP
                     GFGDNSRVVKWIIDRLDGNVDAVDTPIGRVPHPEDLDVSGLGLDPATVERLVTFDVDA
                     WRREVDLIEDHYAFVGERLPAELRDELRDLEKNLDAG"
     gene            2453..2896
                     /locus_tag="VFP02_04755"
     CDS             2453..2896
                     /locus_tag="VFP02_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9542365.1"
                     /translation="MLLQYDPFRELDRVADQAFARATPTMPMDAVRRGDTVHVFLDLP
                     GVDPGSIDLEVERNVLTVKAERRWAREADDQVLANERRQGSFLRRLLLGDTLDGDRVA
                     ADYRDGVLAITIPVAEAAKPRKVSVGTGDSSSTAIEAESNDAAAA"
BASE COUNT          459 a         1067 c          938 g          456 t
ORIGIN      
        1 acaggcggct ggtaccgttc tcccacccca gttgcctgct agtgaggttc ccccggtcat
       61 gcctgcggtc tgtgagatct gtggcaagca tccgtcgttc ggcatgaagc tgagccactc
      121 ccaccgtcgc acgcgtcgtc ggtggaaccc caacatccag agcgtccgtg ccgtcgtgga
      181 cggacgtacc cgccgggtcc gtgcgtgcac ctcgtgcatc cgggcgggca aggtcgtcaa
      241 agccgctcgc tgacgggcgg gggcgacgac gtgcagggag tcatcaagtc ctacgacccc
      301 ggaaccaagg tcggcgtggt ggtcagcgac gctgacctgg ccgagttcga tctgagcgaa
      361 gaggccctca cgggctccat cttccgcacc ctccgactgg gccaacgcgt ggtcttcgac
      421 ctcgacggct cgggttgggc gtccagcgtg cgcatgggct ccgaggtcga cctcggcacc
      481 cccgactacc tcgaggagcg gtgagcgtgt cccccacgac caaccccccg acgtccaacc
      541 gccgactcct ggaatgggtc gacgagtggg cggcgatctt ccagcccgac gccatcgagt
      601 ggtgtgacgg ctccgaggag gagtacggcc gcatgtgccg actcctggtc gagggtggca
      661 ccttcgagac gttgaacccc gagctgcggc cgaacagctt cctgggccgc tccgaccccg
      721 ccgacgtggc tcgggtcgag gaccgcacgt tcatctgctc ggagcgcgag atcgacgccg
      781 gccccaccaa caactggcga ccccccgcgg agatgaaggc cgagatcctc gagttcttca
      841 ccggcgcgat gcggggtcgg accctgtacg tcgtcccctt ctccatgggc cccctcggct
      901 cgcgcatcgc ccacatcggc gtgcagctca ccgactcgcc ctacgtggtg gccaacatgc
      961 ggacgatgac ccggatgggg actccggtcc tcaaggtcct cggcgccggc tcgttcgtga
     1021 agtgcctgca ctcggtcggc tacccgctgg tcgaccggga cgacgtggcc tggccatgtg
     1081 acggcgacaa caagtacatc tcccacttcc cggagagccg cgagatctgg tcgtacggct
     1141 ccggctacgg cggcaacgcc ctgctgggca agaagtgctt cgccctgcgc atcgcgtcgg
     1201 ccatggccca cgacgagggc tggctggccg agcacatgct gatcctgggc gtgaccccgc
     1261 ccgacggcga caccaagtac atcgccgccg ccttcccgtc ggcctgtggc aagaccaaca
     1321 tggccatgat gatccccacc ctcccggggt ggaaggtcga gtgcgtcggg gacgacatcg
     1381 cctggatgaa gttcggcgac gacggccggc tctacgccgt caacccggag gcgggcttct
     1441 tcggtgtggc cccggggacc tccgaagaga cgaacccgaa cgccatgaag accttgggcg
     1501 agaacatcat cttcaccaac gtggccaaga ccgacgacgg cgacgtctgg tgggagggca
     1561 tgagccccgc tcccgaccac ctcgtcgact ggaagggcga ggactggtcg cccgggtccg
     1621 acggcccggc cgcccacccc aacgcccgct tcaccgcccc ggcgtcgcag tgcccggcca
     1681 tctccccgaa ctgggactcg ccggccggcg tgcccatctc ggccatcctc ttcggcggcc
     1741 gccgggccag tgccgtcccc ctggtgaccg aggccaagga ctgggagcac ggggtcttcc
     1801 tcggctcgat catgtcctcc gagaagacgg cggccgccgc cgggacgctc ggcgaggtgc
     1861 gcttcgaccc cttcgccatg ctccccttct gtggctacaa cgtcggcgag tacttcagcc
     1921 actggctgag catcggcgag cacgccaccc atgccccgaa cttgccccgc ctctactggg
     1981 tcaactggtt ccagaaggac gaggacggca agttcctgtg gccgggcttc ggcgacaaca
     2041 gccgggtcgt caagtggatc atcgaccgcc tcgatggcaa cgtcgacgcc gtggacaccc
     2101 cgatcgggcg ggtcccccac cccgaggacc tcgacgtcag cggcctgggg ctggaccccg
     2161 ccaccgtcga gcgcctcgtc accttcgacg tcgacgcctg gcgccgtgag gtcgacctga
     2221 tcgaggacca ctacgccttc gtgggcgagc gcctccccgc cgagctccgg gacgagctcc
     2281 gggacctcga gaagaacctc gacgcgggct gacgccaccc ggcccccgag tttccgcggt
     2341 ttcctgacgg gggttgccgc gcgccgcgga acatgagcaa agttgcgtag taaacgctca
     2401 agtttcttgt taccgtcaga tgggaaacca acgctccctt ggaggtaacc ccatgcttct
     2461 ccagtacgac cccttccgtg agctcgaccg cgtggccgac caggccttcg cccgcgccac
     2521 cccgacgatg cccatggacg ccgtgcgccg cggtgacacc gtgcacgtgt tcctcgacct
     2581 gcctggcgtc gaccccgggt ccatcgacct cgaggtcgag cgaaacgtcc tcacggtcaa
     2641 ggccgagcgt cgctgggccc gcgaggcgga tgaccaggtg ctggccaacg agcgccgcca
     2701 gggctcgttc ctgcgtcggc tgctcctggg cgacacgctc gacggcgatc gcgtggcggc
     2761 cgactaccgc gacggtgtcc tcgccatcac gatcccggtg gccgaggcgg ccaagccccg
     2821 caaggtgagc gtcgggaccg gcgactcctc ctcgaccgcc atcgaggcgg agagcaacga
     2881 cgccgctgcc gcctagctct tcgcgctgtt atcccccttc
//
LOCUS       DASTFV010000251         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_961387, whole genome shotgun sequence.
ACCESSION   DASTFV010000251 DASTFV010000000
VERSION     DASTFV010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961387"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="VFP02_04760"
     CDS             complement(<1..141)
                     /locus_tag="VFP02_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET9542366.1"
                     /translation="MDVAIVGATGQVGAVMRQILAERDFPVDRLRLFASARSVGRTLD
                     WRG"
     gene            complement(201..1415)
                     /locus_tag="VFP02_04765"
     CDS             complement(201..1415)
                     /locus_tag="VFP02_04765"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET9542367.1"
                     /translation="MGLVVQKFGGTSVADAERMRAVADHVALTRKRGDQVVLVVSAMG
                     KETDDLLRLASEVSSDRAGREMDMLITAGERKATALMCMALHEKGVPAESYTGSQAGF
                     ITDTAHTNAKILEIRPDRLRKAIDADVVPVVGGAQGVSTDHDVTFLGRGGSDTTAVAL
                     AQALGADACELYTDVSGVFTADPRLVATARRIDHITFEEMLEMCATGCPKPAMRSVEF
                     ARTHGVRLHVRSAFTWEPGTWITEEDPMEQAIVSGVVDDDSEAKVTVTGVPDRPGIAA
                     QLFRTLADADINVDMIVQNVSEDGVTDISFTLPREALAAGTTITGAIAEEIGAVGVTT
                     DAQIARVSLVGAGMKSNPGVAATMFEVLAGEGINIEMISTSAIRISCVVPEDDLERAV
                     VALHEAFDLAAN"
     gene            1474..1764
                     /locus_tag="VFP02_04770"
     CDS             1474..1764
                     /locus_tag="VFP02_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542368.1"
                     /translation="MATEKRQRQKEGHRLQKEELRKWEQRRRSRRRFLTWGAVIAVAV
                     ALLFGYSALQGDDEDESPTTTAPVTTTIAPATDTTAVTSTTAATPTTTTPAP"
     gene            complement(1776..>2096)
                     /locus_tag="VFP02_04775"
     CDS             complement(1776..>2096)
                     /locus_tag="VFP02_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542369.1"
                     /translation="DGAVVAAASGAARVLCDPMVDPRGGAFTVGLGLLDSLAVVPHHN
                     HWSEEKAHRTLLLAPAGLLVVGIDDATALLRAPDGTWQVAGAGSVAVFRSREDVGLEA
                     LPTA"
BASE COUNT          310 a          738 c          763 g          285 t
ORIGIN      
        1 cccccgccag tccaacgtcc ggccgacgga gcgggcggag gcgaacaggc ggaggcgatc
       61 gaccgggaag tcccgttcgg ccaggatctg gcgcatcact gcgccgacct gacctgtggc
      121 tccgacgatg gcgacgtcca tgcctctcag gctacgagag cctcccggcg gccacgacgg
      181 attaccgatc gtgcggccgc tcagttcgcg gcgaggtcga aggcctcgtg gagggccacg
      241 acggcccgct cgagatcgtc ctcggggacc acacagctga tccggatcgc cgaggtcgag
      301 atcatctcga tgttgatccc ctcaccggcc agcacctcga acatggtggc ggccaccccg
      361 gggttcgact tcatgcccgc accgacgagg ctgacccgtg cgatctgggc gtcggtggtc
      421 acgccgacgg cgccgatctc ctcggcgatg gcacccgtga tcgtggtgcc cgcggccagc
      481 gcctcgcgcg gcagcgtgaa ggagatgtcg gtcacgccgt cctcggacac gttctgcacg
      541 atcatgtcga cgttgatgtc ggcgtcggcc agcgtgcgga acagctgggc ggcgatgccg
      601 gggcggtcgg gcacgccggt gacggtgacc ttggcctcgc tgtcgtcgtc cacgacgccg
      661 gagacgatgg cctgttccat ggggtcctcc tcggtgatcc aggtgcccgg ctcccaggtg
      721 aaggcggagc ggacgtgcag gcgcaccccg tgggtgcggg cgaactcgac cgagcgcatg
      781 gcgggcttgg gacagcccgt ggcgcacatc tcgagcatct cttcgaacgt gatgtggtcg
      841 atgcggcggg ccgtggccac caggcggggg tcggcggtga acacgccgga cacgtcggtg
      901 tagagctcgc aggcatcggc ccccagcgcc tgggccaggg ccacggcggt ggtgtcggag
      961 ccgccccggc cgaggaaggt cacgtcgtgg tcggtggaca cgccctgcgc acccccgacg
     1021 acgggcacga cgtcggcgtc gatggccttg cgcaggcggt cggggcggat ctcgaggatc
     1081 ttggcgttgg tgtgggcggt gtcggtgatg aagccggcct ggctgccggt gtaggactcg
     1141 gcgggcacgc ccttctcgtg cagggccatg cacatgagtg cggtcgcctt gcgctccccg
     1201 gcggtgatga gcatgtccat ctcccgcccg gcccggtccg aggagacctc ggacgcgagg
     1261 cgcagcaggt catcggtctc cttgcccatg gcgctgacca cgagcacgac ctggtccccc
     1321 cgcttccggg tcagggcgac gtggtcggcc acggcccgca tgcgctcggc gtcggccacc
     1381 gacgtgcccc cgaacttctg cacgacgagg cccacgccgt caacgctacc gaggtacgcc
     1441 cgcgggcgac cgcctactaa gttcatcgcc cgcatggcca ccgagaagcg tcaacggcag
     1501 aaggaaggac accgcctcca gaaggaggag ctgcgcaagt gggagcagcg acgtcggagc
     1561 cggcgccgtt tcctcacctg gggcgccgtg atcgcggtcg cggtcgctct gttgttcggg
     1621 tactccgccc tccaggggga cgacgaggac gagtcgccga ccaccacggc gcccgtcacg
     1681 accacgatcg cgccggccac cgacaccacg gcggtgacga gcaccacggc cgccaccccg
     1741 accacgacga cgcccgcgcc ctagccaggg gcggatcaag ccgtcggcag ggcctcgagg
     1801 ccgacgtcct cgcgggaccg gaacaccgcg accgagccgg cgccggcgac ctgccacgtg
     1861 ccgtccgggg cccgcaggag agcggtcgca tcgtcgatgc cgaccacgag cagccccgcg
     1921 ggggccagca gcagcgtgcg gtgggccttc tcctcggacc agtggttgtg gtggggaacg
     1981 acggccaggc tgtcgagcag gccgaggccc accgtgaagg ccccgccgcg ggggtcgacc
     2041 atgggatcgc acaggacccg ggcggccccg ctggccgcgg cgaccactgc gccgtc
//
LOCUS       DASTFV010000252         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_964457, whole genome shotgun sequence.
ACCESSION   DASTFV010000252 DASTFV010000000
VERSION     DASTFV010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964457"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFP02_04780"
     CDS             complement(<1..731)
                     /locus_tag="VFP02_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9542370.1"
                     /translation="MEQLSGRVAVVTGAGSGIGRALVRRFVDDGMSVVAADVEARALD
                     ETVAGTDAVALVCDVSDADAVEALAATTYERFGAAHLVCANAGVFQGGISWERSVEDW
                     QWVLGVNLWGVIHAVKAFVPRMIEGGDEGHVVVTSSRAGLACAAFSGPYQTSKFGAYA
                     VAESLAHDLRAAGIPIGVSALCPGAVNTRIAGSDRNRPGALAAKGGDDVDFVEGALAG
                     LTSQQGIDPSEVAARVVAGVQAGDFL"
     gene            complement(827..1693)
                     /locus_tag="VFP02_04785"
     CDS             complement(827..1693)
                     /locus_tag="VFP02_04785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET9542371.1"
                     /translation="MDFGLFAALAAPYVTTEGLIALGTEAEAHGVESLWVAEHVVLFD
                     EYAPDYPYDENGRFPATADSGILEPLTTLAFLAGQTSTVRLGTGILLLPQRNPVYTAK
                     EVANVDWLSDGRVDLGIGVGWQQEEFAAVNVSWPKRGKRTDEYIAVMRSLWTEDTSTF
                     HGEFYELDRARMYPKPTQAHLPIHIGGESDAAMARAARLGDGWYTFNRLPEDVGPALA
                     RLDELLEQNGRTRADVQISVCPYMHPLTPDMVTAYGEAGVDRVIGMVFGAGPDDFKAG
                     IEALEPCLERAR"
     gene            complement(1715..>2431)
                     /locus_tag="VFP02_04790"
     CDS             complement(1715..>2431)
                     /locus_tag="VFP02_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9542372.1"
                     /translation="GEQLKRYTLELGGKSAAIILDDADVDTTLDNLLQVAGLMNNGQA
                     CVAQTRVLASRARYDEVVSGLAARMAAVKVGDPAEMDTEVGPLVAERQRDRVEKYIVQ
                     GQEEGAEVVVGGGRPAGQDTGWYVEPTLFAKVTNDMRIAQEEIFGPVISAIPFDDVDD
                     AVAIANDSTYGLSGSVWTADAEAGLGIAKRVRTGTFNVNGFMLEPCAPFGGFKQSGVG
                     REGGPEGLAAYLEDKAINRF"
BASE COUNT          344 a          858 c          867 g          362 t
ORIGIN      
        1 aggaagtcgc cggcctgcac accggcgacg acccgggcgg cgacctcgga cgggtcgatg
       61 ccctgctggc tggtcaggcc ggccagggcg ccttcgacga agtcgacgtc gtcgccgccc
      121 ttggccgcca gcgcgccggg gcgattgcgg tcggagccgg cgatgcgggt gttgacggcg
      181 ccggggcaga gggcggacac gccgatcggg atgccggccg cccgcaggtc gtgggccagg
      241 gactcggcca cggcgtaggc cccgaacttg gacgtctgat agggcccgct gaaggcggcg
      301 caggccaggc cggccctcga ggacgtgacc acgacgtggc cctcgtcgcc cccctcgatc
      361 atgcggggga cgaacgcctt caccgcgtgt atgacgcccc agaggttcac gccgagcacc
      421 cactgccagt cctcgacgct gcgctcccag ctgatcccgc cctggaacac gccggcgttg
      481 gcgcacacca ggtgggcggc accgaaccgc tcgtaggtgg tcgcggccag agcctcgacg
      541 gcgtcggcat cgctgacgtc gcacaccaac gcaacggcgt ccgttccggc caccgtctcg
      601 tccagggccc gcgcctcgac gtcggccgcg accaccgaca tgccgtcgtc cacgaaccgg
      661 cgcaccaggg cccgcccgat ccccgacccg gcccccgtga ccacggccac ccgcccgctc
      721 agctgctcca tgacccgacc gtaggacacc ggcggatccg ccccgttctg gcctctgatc
      781 tgcttgccct ggccggagga tcagaggcca gaacgagggg ggagggtcat cgggcgcgct
      841 cgaggcaggg ctcgagggct tcgatgccgg ccttgaagtc gtcggggccg gcgccgaaca
      901 ccatgccgat gacccggtcg actccggctt cgccgtaggc ggtgaccatg tcgggggtca
      961 gggggtgcat gtagggacag acgctgatct gcacgtcggc ccgggtccga ccgttctgct
     1021 ccagcagctc gtcgagccgg gcgagggcgg gcccgacgtc ctcgggcagg cggttgaagg
     1081 tgtaccagcc gtcgccgagg cgggcggctc gggccatggc ggcgtcggat tcgcccccga
     1141 tgtggatcgg caggtgggcc tgggtcggct tcgggtacat gcgggcccgg tcgagctcgt
     1201 agaactcgcc gtggaaggtc gacgtgtcct cggtccacag cgagcgcatc accgcgatgt
     1261 actcgtcggt ccgcttgcct cgcttcggcc aggagacgtt caccgcggcg aactcctcct
     1321 gctgccagcc cacgccgatg cccaagtcga cgcgtccatc cgacagccag tcgacgttgg
     1381 ccacctcctt ggccgtgtag acggggttgc gctgcggtag cagcaggatg cctgtgccga
     1441 gacggaccgt ggaggtctgg ccggccagga acgccagcgt ggtcagcggt tcgagaatgc
     1501 cggagtcggc cgtcgccggg aagcgcccgt tctcgtcgta ggggtagtcg ggcgcgtact
     1561 cgtcgaagag cacgacgtgc tcggccaccc acaacgactc gacaccgtgg gcctcggcct
     1621 cggtcccgag ggcgatcagg ccctcggtgg tgacgtaggg cgcggcgagc gcggcaaaga
     1681 gaccgaagtc catgtcatgt ccccctggtg gtggtcagaa ccggttgatg gccttgtcct
     1741 cgaggtaggc ggccaggccc tcgggcccgc cctcccgacc cacgccggac tgcttgaacc
     1801 cgccgaacgg ggcgcagggc tcgagcatga agccgttcac gttgaacgtg ccggtgcgga
     1861 cccgtttggc gatgccgagg ccggcctcgg cgtccgcggt ccagaccgag cccgacaggc
     1921 cgtaggtcga gtcgttggcg atggccacgg cgtcgtcgac gtcgtcgaag gggatggccg
     1981 agatgaccgg cccgaagatc tcctcctggg cgatgcgcat gtcgttggtg accttggcga
     2041 agagggtggg ctcgacgtac cagcccgtgt cctgaccggc ggggcgaccg ccgcccacga
     2101 cgacctccgc accctcctcc tggccctgca cgatgtactt ctcgacccgg tcccgctgac
     2161 gttcggcgac gagagggccg acctcggtgt ccatctcggc cgggtccccg accttgaccg
     2221 cggccatgcg agcggccagg ccgctgacga cttcgtcgta cctggcccgg ctggccagca
     2281 cccgggtctg ggccacgcac gcctgaccgt tgttcatcag gccggccacc tgcagcaggt
     2341 tgtccagcgt ggtgtcgacg tcggcgtcgt cgaggatgat cgccgccgac ttgccgccca
     2401 gctcgagggt gtagcgcttg agctgctcgc c
//
LOCUS       DASTFV010000253         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_965386, whole genome shotgun sequence.
ACCESSION   DASTFV010000253 DASTFV010000000
VERSION     DASTFV010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965386"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VFP02_04795"
     CDS             <1..504
                     /locus_tag="VFP02_04795"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9542373.1"
                     /translation="TLVLVGELDRLSREDSLTGVANRRAWDEALKRSCAEAERTGLPL
                     SVLLLDIDRFKEINDEHGHRRGDEVLQQVSECLSGRVRASDLLARVGGDEFAVLCPNT
                     DPGAARHLADHLIDLVAAMPGDFSVSVGSAVRWPGAEPDAVMGLADRRLYQAKRARVN
                     SGSATGR"
     gene            522..779
                     /locus_tag="VFP02_04800"
     CDS             522..779
                     /locus_tag="VFP02_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HET9542374.1"
                     /translation="MCRNITTLRGLEPVATDEEIEAAARQYVRKISGVQKTSAATEEA
                     FEKAVAVITAASHEVLEALPPRKNPPPTLPPLRRLAAAKAG"
     gene            complement(809..893)
                     /locus_tag="VFP02_04805"
     tRNA            complement(809..893)
                     /locus_tag="VFP02_04805"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(857..859),aa:Ser,seq:gga)
     gene            967..1065
                     /gene="ffs"
                     /locus_tag="VFP02_04810"
     ncRNA           967..1065
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFP02_04810"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1129..>2872
                     /gene="dnaX"
                     /locus_tag="VFP02_04815"
     CDS             1129..>2872
                     /gene="dnaX"
                     /locus_tag="VFP02_04815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET9542375.1"
                     /translation="MAFQSLYRRYRSQRFSEIVGQEHVVAALRNAAAEDRLGHAYLFS
                     GPRGTGKTSTARILAKVLNCENPVDGEPCGVCPPCLAIEAGNSFDVTELDAASHTGVD
                     NMRELIASTALASPGRTRLYILDEVHMLSKGASAALLKTLEEPPDHVVFILATTDPEK
                     VLPTIRSRTQHLEFRLLPAAELEAHVRWIVEDAGLEVTEEGIAQAMREGGGSARDTLS
                     VLDRIVAAGGVARDDDSVDDLLDALCENDTARALTALADATAQGRDPRGVGEQVLERL
                     RNAFLAAMGVDLDHLTEGDRAAAADHAERLRAPAITRALEVLGTALVDMRQAPDPRIP
                     LEVALVRITRAASDTSVDALLIRIDRLEQVVRDGNGGELLAPADDPAASTGGGSPANE
                     ARSRLAEVRRPDTPEPVVAPEPSHPPLAAVPDPAPEPPAVVAAVPEPAPTPGATGSDA
                     LPTRDELTLAWGDRVLGGLRPKVKMLFAAGRFLDDREGQAAFALPNATHVQRCEPLRA
                     DVEAALAAEFGRAVPLILVVDGGADSPPAPAPTSPAGPDDRPTERSPAPTPVVDELAD
                     IGPVDDLEDAGSAST"
BASE COUNT          395 a         1084 c         1006 g          387 t
ORIGIN      
        1 accctcgtgc tggtcggcga actggatcgc ctcagccggg aggactcgtt gaccggtgtg
       61 gccaaccggc gggcgtggga cgaagccctg aagcggtcgt gcgccgaggc cgagcggacg
      121 ggcctacccc tctcggtcct cctcctcgac atcgatcgct tcaaggagat caacgacgag
      181 cacggccacc gccggggcga cgaggtcctc cagcaggtct ccgaatgcct gtccgggcgc
      241 gtccgcgcct cggacctgct ggcccgggtc ggcggcgacg agttcgccgt gctctgcccc
      301 aacaccgacc ccggggccgc ccgccacctg gccgaccact tgatcgatct ggttgccgcc
      361 atgccgggcg acttcagtgt ctcggtcggc tcggccgtcc ggtggcccgg tgccgagccc
      421 gacgcggtga tgggcctggc cgaccggcgc ctctaccagg ccaagcgggc ccgggtgaac
      481 agcggcagtg caaccggccg ctgatcggta gcgtcgcggt catgtgccgc aacatcacca
      541 ccctccgggg cttggagccc gtggccaccg atgaggagat cgaggccgcg gcccgtcagt
      601 acgtccgcaa gatcagcggg gtccagaaga cctcggccgc caccgaggag gccttcgaga
      661 aggcggtggc cgtgatcacg gccgcttccc acgaggtcct cgaggcgctt ccgccccgca
      721 agaacccgcc gccgacgctg cccccgctgc gtcgactggc cgctgcgaag gcgggctgag
      781 cccgaggccg ctcggccgag gacctgcgcg gagggggtgg gattcgaacc cacggtgggt
      841 tgccccacac acgctttcca agcgtgccga ttcggccgct ctcgcacccc tcctggggac
      901 acccgcccgt ggggctggtg cggccggtca gggtatcgtg ggcgctcgct cgccttggag
      961 tgtggcggtc gggtcctgtg cgacggtggc ccgtgaaccg agtcagggcc ggaaggcagc
     1021 agctctcagc ggagcccacc gggtgccgca gatcgcctgg ccgccactct cgaaggcgag
     1081 cggggaaggc cgaacgagcg gcgagtcacc gcttgtagtc tccgggcgat ggccttccaa
     1141 tcgctgtatc ggcgctaccg gtcgcagcgg ttctcggaga tcgtgggcca ggagcacgtg
     1201 gtcgccgccc tccgcaacgc cgccgccgag gaccggctgg ggcacgccta tctcttcagc
     1261 gggccccggg ggacgggcaa gacctcgacc gcccgcatcc tggccaaggt cctcaactgc
     1321 gagaatcccg tcgacggcga gccctgtggg gtctgcccac cctgcctggc catagaggcg
     1381 ggcaactcct tcgacgtgac cgagctggat gccgcctcgc acacgggtgt cgacaacatg
     1441 cgcgagctca tcgccagcac ggcgttggcc agcccgggtc ggacccgcct ctacatcctg
     1501 gacgaggtcc acatgctgtc gaagggtgcg tcggccgccc tcctcaagac cctggaggag
     1561 ccgccggacc acgtggtgtt catcctggcc acgaccgatc ccgagaaggt ccttccgacg
     1621 atccgcagcc ggacccagca cctcgagttc cgactgctcc cggcggccga gctggaggcc
     1681 cacgtccgct ggatcgtgga ggacgccggc ctcgaggtca ccgaggaggg catcgcccag
     1741 gccatgcgcg aaggcggcgg ctcggctcgc gacaccctct ccgtcctcga ccgcatcgtg
     1801 gccgccggcg gggtggcccg cgacgacgac tcggtcgacg acctgctcga cgccctgtgc
     1861 gagaacgaca cggcccgcgc cctcaccgca ctggctgacg ccaccgccca gggccgtgac
     1921 ccccgggggg tcggcgagca ggtgctggaa cggctgcgca acgccttcct cgccgcgatg
     1981 ggggtggacc tggaccacct caccgaaggg gaccgggccg ccgcggccga ccacgccgag
     2041 cgcctccggg cccccgccat cacccgggcg ctggaggtcc tgggcacggc gctggtcgac
     2101 atgcgccagg ccccggaccc ccgcatcccc ctcgaggtgg ccttggtccg catcacccgg
     2161 gcggcgagtg acacctccgt cgacgcgctg ctgatccgca tcgaccgtct cgagcaggtc
     2221 gtccgggatg gcaacggcgg cgagcttctg gcgccggccg acgacccggc cgcgtcgacc
     2281 ggtggaggat caccggcgaa cgaggcccgt agccggctgg ccgaggtgcg ccggcccgat
     2341 accccggagc cggtggttgc ccccgagccg agccatcccc cgctggccgc cgtccccgat
     2401 ccggcgccgg agccgccggc cgttgtcgcg gccgtgcctg agccggcacc gactccagga
     2461 gccaccgggt cggacgccct cccgacgcgg gacgaactca ccctggcctg gggtgaccgg
     2521 gtcctcggcg ggctgcggcc caaggtcaag atgctgttcg ccgccggtcg cttcctcgac
     2581 gaccgcgagg gccaagccgc tttcgccctg cccaacgcca cccacgtcca gcgctgtgag
     2641 ccgctgcggg ccgacgtcga ggccgcgctg gccgcggagt tcggccgtgc cgtcccgttg
     2701 atcctcgtcg tcgacggtgg tgccgattcc cccccggccc cggccccgac ttcgccggca
     2761 ggaccggacg atcggcccac ggaacggtca ccggctccca cgccggtggt ggacgagttg
     2821 gccgacatcg gtccggtcga cgacctggag gacgccggat cggcctcgac cg
//
LOCUS       DASTFV010000254         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_967295, whole genome shotgun sequence.
ACCESSION   DASTFV010000254 DASTFV010000000
VERSION     DASTFV010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967295"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VFP02_04820"
     CDS             complement(<1..927)
                     /locus_tag="VFP02_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542376.1"
                     /translation="MPALKTEITEIVTGLAMCGRADLDIALQHRPPELLNVDDATWGR
                     ITAAWERRELGQEFFAAWANGFAFLKARSGLRGKAPLRVEWKGPHKAPGDERVPADLR
                     VDHVYLISCKYLSSILHNASPSALFDHRLGATDERIANWYAEVAPAEFQALYESVRND
                     ALPARATDLTRDERRALSETLKPGWPDGALARYEELTTVVAAASATRWRHQLGKALAR
                     EEMAWRLLRLQPAPYFVLGTTDAAILRVRVGTPWDWRQRFDLQAFDVAAEAGGQPRVS
                     WRVEVHDREADVQRVAAGHVEVRWSHGRFGQPP"
     gene            1054..1962
                     /locus_tag="VFP02_04825"
     CDS             1054..1962
                     /locus_tag="VFP02_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542377.1"
                     /translation="MARTCRTRHGPVRDLDAVARQLDAIAEEAGWGGPPMLIGLTEEG
                     VDDVNPPAPVPGSSGTDPVDLVASLVGFDAPAEWEAMAVVVQGRSWALDDRSADPRPV
                     RLTHVVDRHGGLASVIRHAGEEPTVRTETGEGRLIDVCRRCLGLTTAPPPSDSTALWA
                     LLWLDHLLARAARGEPLRGLMAAARAHPGVEMVAEHEPKLVDMAAERLVRIGRIMGEA
                     RNWPHLRRAASAGEWPVENLAPDGAAWMDDGMFARWVMAEFPLLDEYLAELDRLLPES
                     TVTAVRAVLAEWDLLDPPGDPASALS"
     gene            complement(1955..>2914)
                     /locus_tag="VFP02_04830"
     CDS             complement(1955..>2914)
                     /locus_tag="VFP02_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542378.1"
                     /translation="LTAVALVLVVGRGVLLDSDAHELEVLLDQVEAEAEGPRDLTPYD
                     GLGTWVDAYDYGPAYQTDGHEPAVTPDDVAAMDAAGVRTVFLQVNRDDERSPDGVVDR
                     DLVTEFVTEAHERDMAVVGWYLPTFRSVAVDLGHLQDLLDFDADGQRLDGVAVDIEFT
                     EAVPNAALRSRRLVRLSERLAEAAGGDPIGAIVLPPVLTEVVSPDFWPRFPWSDISEL
                     YDVWLPMSYWTLRTEGSGYRDGATYHEESVRRMEANLGRADLVVHGIGGIGDETTGED
                     LLAFAETLSAMGAVGGSIYDWATLDQDDRLLLRRLFDEYPEIN"
BASE COUNT          384 a         1078 c         1042 g          410 t
ORIGIN      
        1 agggggttga ccgaagcggc cgtggctcca gcggacctcg acgtgccccg ccgccacccg
       61 ctgcacgtcg gcctcccggt cgtggacctc cacgcgccag gacacccggg gctggcctcc
      121 ggcctcggcc gccacgtcga aggcctgcag gtcgaagcgc tggcgccagt cccagggcgt
      181 ccccacccgg acccggagaa tggccgcatc cgtcgtgccg aggacgaagt acggcgccgg
      241 ctgcaggcgc aacagccgcc acgccatctc ctcacgggcc agtgccttcc cgagctggtg
      301 gcgccaccgc gtggctgagg cggcggccac gacggtggtc agctcctcgt agcgggccag
      361 cgcgccatcc ggccatccgg gcttgagggt ctcgctcagc gcccgacgct cgtcacgggt
      421 caggtccgtg gcccgagccg ggagggcgtc gttgcggacc gactcgtaga gcgcctggaa
      481 ctcggccggc gccacctcgg cgtaccagtt ggcgatccgc tcgtcggtgg cgccgagccg
      541 gtggtcgaac aaggccgacg gcgaggcgtt gtgcaggatc gacgacaggt acttgcagct
      601 gatcaggtag acgtggtcga cccgcaggtc ggcgggcacc cgctcgtcgc cgggcgcctt
      661 gtgcggtccc ttccactcca cccgcagcgg cgccttgccc cgcagcccgc tgcgggcctt
      721 gaggaaggcg aacccgttgg cccacgccgc gaagaactcc tggcccagct cccgccgctc
      781 ccaggccgcg gtgatgcgtc cccacgtggc gtcgtcgacg ttcagcagct cgggcggccg
      841 gtgctgcaac gcgatgtcga ggtcggcccg gccgcacatc gccagcccgg tgacgatctc
      901 ggtgatctcg gtcttcaggg cgggcacccg ctgggttgta gcagcagcgc ccgactgtca
      961 cagggtccgg gcagactcgc ggttggttcg cgacccgacc tcacgggcgc cttcgaggcc
     1021 ctcccagcgg gaggccacac cccggaggcc cccatggccc gcacctgtcg cacccgtcat
     1081 ggtcccgtcc gtgacctgga cgccgtcgcc cgtcagctcg acgccatcgc cgaggaggcg
     1141 ggctggggcg gtccgccgat gctcatcggc ctgaccgagg agggcgtgga cgacgtgaac
     1201 ccgcctgccc ccgtccccgg ctcgtcgggc accgatcccg tcgacctggt ggcctccctg
     1261 gtcggcttcg acgccccggc cgagtgggag gccatggccg tcgtggtcca gggccgctcc
     1321 tgggccctcg acgaccgttc cgccgacccg cgtccggtgc gcctgaccca tgtggtcgac
     1381 cgccatgggg gcctggcctc cgtcatccgc cacgcgggtg aggagccgac ggtccgaacg
     1441 gagaccggcg agggacgcct gatcgacgtc tgccgccgct gccttggcct gaccacggcc
     1501 ccgcctccgt cggacagcac cgcgctgtgg gccctgctct ggctcgacca ccttctggcc
     1561 cgggccgccc ggggcgagcc gctgcggggc ctgatggccg ccgcccgggc tcatcccggc
     1621 gtggagatgg tcgcggagca cgaaccgaag ctggtcgaca tggcggccga gcgcttggtc
     1681 cgaatcgggc ggatcatggg cgaggcccgc aactggcccc acctgcgccg ggcagcgtcg
     1741 gcgggcgaat ggccggtcga gaacctcgcc cccgacggtg cggcctggat ggacgacggc
     1801 atgttcgccc gctgggtgat ggccgagttc cccctgctcg acgagtacct cgcggagctg
     1861 gaccgtctcc tgcccgagag caccgtcacc gccgtgcgcg ccgtcttggc cgaatgggac
     1921 ctgctcgacc cacccgggga cccggcgtcc gcgctcagtt gatctcgggg tactcgtcga
     1981 agaggcggcg caggaggagc cggtcgtcct ggtccagggt ggcccagtcg tagatcgagc
     2041 cgccgaccgc acccatggcc gagagcgtct cggcgaaggc caggaggtcc tcacccgtcg
     2101 tctcgtcgcc gatgccgccg atgccgtgga cgaccaggtc ggcccggccg aggttcgctt
     2161 ccatccggcg gacgctctcc tcgtggtacg tcgccccgtc ccggtagccg gagccctccg
     2221 tgcgcagcgt ccagtagctc atgggcagcc agacgtcgta cagctcgctg atgtcgctcc
     2281 acgggaaccg gggccagaag tcggggctga ccacctcggt caacacgggg ggcaggacga
     2341 tggcgccgat cgggtcccca ccggctgctt cggccagccg ctcggacagg cggaccaggc
     2401 gacggctgcg cagggcggcg ttgggcacgg cctcggtgaa ctcgatgtcc acggccacgc
     2461 cgtcgaggcg ctggccgtcc gcgtcgaagt cgagcaggtc ctggaggtga ccgaggtcga
     2521 cggcaacgga ccggaacgtc ggcaggtacc agccgaccac cgccatgtcc cgttcgtggg
     2581 cctcggtcac gaactcggtc accaggtccc ggtcgacgac gccgtcgggg gagcgctcgt
     2641 catcccggtt gacttgcagg aacacggtgc gcacgccggc tgcgtccatg gcggccacgt
     2701 cgtcgggggt gactgccggc tcgtggccat cggtctggta cgccggtccg tagtcgtagg
     2761 cgtcgaccca ggtgcccagc ccgtcgtagg gggtcaggtc ccggggaccc tcggcctcag
     2821 cctcgacctg gtcgaggagc acctccaact cgtgggcgtc cgagtcgagc agcaccccgc
     2881 gcccgacgac gagcacgagg gccaccgcgg tgag
//
LOCUS       DASTFV010000255         3120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_968610, whole genome shotgun sequence.
ACCESSION   DASTFV010000255 DASTFV010000000
VERSION     DASTFV010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968610"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VFP02_04835"
     CDS             complement(<1..492)
                     /locus_tag="VFP02_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542379.1"
                     /translation="MPLFRRNAGRPRFRYSRWDGTQVGFELGADEVMAEITDDLLYHG
                     DLNAALRRMLQSGFDTPDGEHLQGLREIMERLRQQRREMLENHDLGGVYDEIADELRE
                     VIDQERASLQELARDAAESGNQRRQEVTDEVVQQRQMDLDLLPPDLAGQVKALQEYEF
                     TSSE"
     gene            complement(497..1900)
                     /locus_tag="VFP02_04840"
     CDS             complement(497..1900)
                     /locus_tag="VFP02_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9542380.1"
                     /translation="MSSRPATIGQLRESGWESVPVKEEVRRNAVARILAGESIVEGVL
                     GFEETVLPQLENAVLAGHDIIFLGERGQAKTRMIRSLTDLLDEWLPIIAGSEILDDPY
                     DPVSRHARDLMADHGEDTPIEWVHRDDRFGEKLATPDTSIADLIGEVDPIKVAEGRYL
                     SDELTMHYGLVPRTNRGIFAINELPDLSERIQVGLLNVLEERDIQVRGYKIRLPLDVL
                     LVASANPEDYTNRGRIITPLKDRFGSQIRTHYPLDVETEVAIARQEARPFDVDGVRVD
                     VPEFMAEVVATLSQLARESPHVNQSSGVSVRLTVANMEVLVANATRRALRLGETDVVP
                     RITDLDALASSTSGKIEIETLEEGRDGQIIDQLLRGAILTTFRETFDLGELSGVLDAF
                     EEARAVDAGEDIGSAAYAELAAEIPALREGVNRLTEDESPAAVASAVEFILEGLHLSK
                     RLNKEAAGGRATYRGRG"
     gene            1919..>3120
                     /locus_tag="VFP02_04845"
     CDS             1919..>3120
                     /locus_tag="VFP02_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HET9542381.1"
                     /translation="MNLPADWRVAPTDPLLERGFADPDLDDSDWESLTVPGHWRSSAA
                     FAEADGPLLYRVPFTADHPVDGERAWLVFDGVFYQSDVWLDGGYVGDTEGYFFGHTFE
                     VTDQLRDRAEHLLAAQVVCSPPGDRTAKRSLTGVFQHWDCIDPDLNPGGIWRPVRIER
                     TGPIRLRRLSVVCTEAGGESATLRFRAFADTAAPGIVKIATTVGRDGVADDPYTEELS
                     LAAGENQLEWLVTVPEPKLWWPWALGHQDRYDVTVEAIGEDGAISHTLERTVGLRSLV
                     FNDWQLSVNGERLFLKGANLAPTRALLGEATDEDVRRDIDLARDAGLDLVRVHAHIAT
                     PALYDQADERGMLVWADLPLQWGYARSVRKQAVRQARKAVEILGHHPSIAIWCGHNEP
                     LALDVTTA"
BASE COUNT          443 a         1087 c         1114 g          476 t
ORIGIN      
        1 ctccgaggag gtgaactcgt actcctgcag cgccttcacc tggccggcga ggtcaggggg
       61 caggaggtcg aggtccatct ggcgctgctg gacgacctcg tcggtcacct cctggcgccg
      121 ttggttcccc gactcggctg cgtcccgggc cagctcctgc agcgaggccc gctcctggtc
      181 gatgacctcc cggagctcgt cggcgatctc gtcgtagacc ccgccgaggt cgtggttttc
      241 gagcatctcc cggcgctgct ggcgcaggcg ttccatgatc tcccgcaggc cctggaggtg
      301 ctcgccgtcg ggggtgtcga agcccgactg gagcatgcgg cgcagggcgg cgttgaggtc
      361 accgtggtac aggaggtcgt cggtgatctc cgccatgacc tcgtcggcgc cgagctcgaa
      421 gccgacctgg gtgccgtccc agcgcgagta acggaaccgg ggccgtcccg cattgcgccg
      481 gaagagcggc atcgccctag ccccgccccc ggtaggtggc gcggccgccg gcggcttcct
      541 tgttgaggcg cttggagagg tgcaggccct cgaggatgaa ctcgacggcg gaagccaccg
      601 cggccggtga ctcgtcctcg gtgagccggt tcacgccttc gcggagggcc ggtatctcgg
      661 cggccagctc ggcgtaggcc gccgagccga tgtcctcacc cgcgtccacg gcccgggctt
      721 cctcgaaggc gtcgaggaca ccggacagct cgccgaggtc gaacgtctcg cggaacgtgg
      781 tcaggatcgc gccccgcagg agctggtcga tgatctggcc gtcgcggccc tcctcgaggg
      841 tctcgatctc gatcttgccc gaggtcgagg aggccagggc atcgaggtcg gtgatgcggg
      901 gcacgacgtc ggtctcaccg aggcgcagcg cccgccgggt ggcgttggcc accagcacct
      961 ccatgttggc cacggtcagg cggaccgaca cgcccgagga ctggttcacg tgggggctct
     1021 cgcgggccag ttgggagagc gtggcgacga cctcggccat gaactcgggc acgtccaccc
     1081 gcacgccgtc cacgtcgaag ggccgggcct cctgccgggc gatggccacc tcggtctcga
     1141 cgtcgagcgg gtagtgggtg cggatctggc tgccgaagcg gtccttcagc ggcgtgatga
     1201 tccgaccccg gttggtgtag tcctccgggt tggccgaggc cacgagcagc acgtcgaggg
     1261 ggaggcggat cttgtagccc cggacctgga tgtcgcgctc ctccaggacg ttgagcaggc
     1321 cgacctggat gcgctcggag aggtcgggga gctcgttgat ggcgaagatg ccgcgattgg
     1381 tccggggcac caggccgtag tgcatcgtca gctcgtcgga gagatagcga ccctccgcca
     1441 ccttgatggg gtcgacctcg ccgatcaggt cggcgatgga cgtgtccggc gtggccaact
     1501 tctcgccgaa gcggtcgtca cggtggaccc attcgatggg cgtgtcctca ccgtggtcgg
     1561 ccatcaggtc ccgggcgtgg cgggagaccg ggtcgtaggg gtcgtccagg atctcggagc
     1621 cggcgatgat cggcagccac tcgtcgagga ggtcggtgag ggagcggatc atgcgggtct
     1681 tcgcctggcc ccgctcgccc aggaagatga tgtcgtgacc ggccaggacg gcgttctcca
     1741 gctgcgggag gacggtctcc tcgaagccca ggacgccctc gacgatcgac tcgccggcga
     1801 ggatgcgggc cacggcgttg cgccggacct cctctttgac cggaaccgat tcccagccgg
     1861 actcgcggag ctgaccgatc gttgcgggac gagacgacat agctggcaac ctacccgggt
     1921 gaatctgccg gcggactggc gggtggcccc gaccgacccg ctcctggaac ggggcttcgc
     1981 cgacccggac ctcgacgact cggactggga gtccctcacc gtccccgggc actggcgtag
     2041 cagcgcggcc ttcgccgagg cggacgggcc gctgctctac cgggtcccct tcacggccga
     2101 ccaccccgtc gacggcgagc gggcctggct ggtgttcgac ggcgtcttct accagtccga
     2161 cgtctggctg gacggcggct acgtgggcga caccgagggg tacttcttcg gccacacgtt
     2221 cgaagtgacc gaccagctgc gggaccgggc cgagcacctc ctggccgccc aggtcgtctg
     2281 ctcgccgccc ggcgaccgca ccgccaagcg cagcctgacc ggcgtcttcc agcactggga
     2341 ctgcatcgat cccgacctga acccgggcgg gatctggcgg ccggtccgca tcgagcggac
     2401 ggggccgatc cgactccgac ggctctcggt ggtctgcacc gaggccggtg gcgagtccgc
     2461 cacgctccgc ttccgggcct tcgccgacac cgcggcgcct ggcatcgtca agatcgccac
     2521 caccgtcggc cgagacggtg tggccgacga tccctacacc gaagagctca gcctggccgc
     2581 gggcgagaac cagctggaat ggctggtgac cgtgcccgag ccgaagctct ggtggccgtg
     2641 ggccctcggc caccaggacc gctacgacgt cacggtcgag gccatcggcg aggacggggc
     2701 catcagccac accctcgagc gcacggtcgg cctgcggtcg ctggtgttca acgactggca
     2761 gctgtcggtc aacggcgagc gcctcttcct caaaggggcc aacctggccc cgacccgggc
     2821 cctgctgggc gaagccaccg acgaggacgt ccggcgagac atcgacctgg cccgcgacgc
     2881 cggcctcgac ctggtgcggg tccacgccca catcgccacc ccggccctct acgaccaggc
     2941 cgacgagcgg gggatgctcg tgtgggccga cctgccgctc cagtggggct acgcccgctc
     3001 cgtgcgcaag caagcggtgc gccaggcccg caaggcggtc gagatcctcg gtcaccatcc
     3061 gtcgatcgcc atctggtgcg ggcacaacga gccgctggcg ctcgacgtga ccaccgccca
//
LOCUS       DASTFV010000256         3460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_970016, whole genome shotgun sequence.
ACCESSION   DASTFV010000256 DASTFV010000000
VERSION     DASTFV010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970016"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..260)
                     /locus_tag="VFP02_04850"
     CDS             complement(90..260)
                     /locus_tag="VFP02_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542382.1"
                     /translation="MLGFSIVNFSAYYETHVTEDGDFEVSLPTKVPVNLEEEMDFDAD
                     AIRSGVRIAEFY"
     gene            513..1742
                     /locus_tag="VFP02_04855"
     CDS             513..1742
                     /locus_tag="VFP02_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET9542383.1"
                     /translation="MSEATPNVREARRMLGKGIVIAGVGETEQGKLEGRGSFQLLAEV
                     TKITLDDAGITLDEVDGLVTAFSFVESTLMHATTFADYLGMEPGYFASVAVGGATAPL
                     MAVQAALAIDAGLAETVLCVRGDNTRSGISSSGMIAMAREMSHGAFEAPYGLTTIGGY
                     GLLAQRYMYEHDVPQEALAAVVATQSEHAALKWNAMLRDVVTVEDVLNDRWIATPFHA
                     MDCSLVSDGAAAFLVTTADRAAAMRAKPVYITGIGEGYSHQYISSASSVDGVRQGLGR
                     AGRRAMEVAGITPADLDVAEIYDSFSFTPLIALEGFGVCGPGEAGDWVLAGNAKLGGK
                     LPMNTHGGLIRQAHLGAMHHIVEAALQLRGEADDSDRGGGNHQVPGAKLAITNGSGGI
                     LAATSALVLSSEPPSAA"
     gene            1765..2208
                     /locus_tag="VFP02_04860"
     CDS             1765..2208
                     /locus_tag="VFP02_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HET9542384.1"
                     /translation="MPDEATKPAYTKPLPDLRPEGERFWEGTREHKLLLPTDAQGKPF
                     WYPRALVPGALEPVGWVEAAGTGEVYTYSVHFIGPSKAYKGDPPHVVALIDLDEGVRM
                     MSNLVRDESPDYPSIDPDDVSIGMRVRVVFHDVTDEITLPKFVPA"
     gene            2256..>3460
                     /locus_tag="VFP02_04865"
     CDS             2256..>3460
                     /locus_tag="VFP02_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9542385.1"
                     /translation="MAEWSDWYAREDPTQWVLAEILKRRASETPDRDYLKFAENPWVS
                     YGEVNARANRVANALIDRGVEPGESVSVMLPNCEEFLPVWYGILKAGAVMSSINTAYK
                     GDFLSWTINLVEAKKLVISDLYLDRLDLIKGELPKLEHVIVMQTGAQEGPDPSMAWEP
                     LAELMAATDGEPDGVSYSWTDDARIMFTSGTTGRSKGVIKQNAADYFSARGLLEVVSA
                     TAGKSVESLEEDTFFSCLPLFHSNAQVLSGYPALVAGGRVAYVERFSSSRFWQQVIDA
                     DATVFNSIGAVSYFIWNIPASELDRAHTVHTCFAAPAPKDIYNEFQERFGVKFIEGYG
                     LTETGMATYMDPTKPAVPGSMGKANPGYEVSIVEPGTDRPLPPETPGEIVVDMKIPNI
                     VMRAYYGMP"
BASE COUNT          551 a         1208 c         1220 g          481 t
ORIGIN      
        1 aacccgatgg ccaaggtctt cccgatggtc ccccagggca aggggcccgg cgcgatcatc
       61 gtcggcggcc ccgccgccgg ggcgagcgcc tagtagaact cggcgatgcg gacgcccgag
      121 cggatcgcgt cggcgtcgaa gtccatctcc tcctcgaggt tgaccggcac cttggtcggc
      181 agcgacacct cgaagtcgcc gtcctcggtg acgtgggtct cgtagtaggc ggagaagttg
      241 acgatcgaga agccgagcac gtggttggtg tccgggtcga agtagaccgt gaggccctcg
      301 gagttggtga cggccgaggc gttgtcgtgg ccgcggtaga tcaggcggta ggtgtcggcc
      361 tcggggtcgt agttggggtc gcggtcggac agcgcgggcc tccttccggt cgatgggtcg
      421 gtcgccgccg gacggcggcg cggcgctggt agcgtagcgg cccgcagggg ccgcccggcc
      481 ccctgccgat cgcacgcgac agaggaggat ggatgagcga ggccacgccc aacgtccgcg
      541 aggcgcggcg gatgctcggc aagggcatcg tgatcgccgg ggtgggggag accgagcagg
      601 gcaagctcga gggacgcggg tccttccagc tgctggccga ggtcaccaag atcaccctcg
      661 acgacgccgg catcacgctg gacgaggtcg acgggttggt caccgccttc tccttcgtcg
      721 agtcgacgct gatgcacgcg acgaccttcg ccgattacct gggcatggag cctggctact
      781 tcgcctcggt tgccgtcggt ggggcgaccg cgccgctgat ggcggtgcag gccgccctgg
      841 cgatcgacgc gggcctggcc gagacggtgc tctgcgtgcg cggcgacaac acccgctcgg
      901 gcatcagctc gtcggggatg atcgccatgg cgcgcgagat gagccacggg gccttcgagg
      961 ccccctacgg cctgacgacg atcggcggct acgggctgct ggcccagcgc tacatgtacg
     1021 agcacgacgt gccccaggag gcgctggcgg cggtcgtcgc cacccagtcc gagcacgccg
     1081 ccctgaagtg gaacgcgatg ctgcgcgacg tggtcacggt cgaggacgtc ctcaacgacc
     1141 gctggatcgc gaccccgttc cacgccatgg actgctcgct cgtcagcgac ggggcggcgg
     1201 ccttcctggt caccaccgcc gaccgggcgg ccgcgatgcg cgccaagccg gtctacatca
     1261 ccggcatcgg cgagggctac tcgcaccagt acatctcctc ggcctcctcg gtggacggcg
     1321 tccgccaggg cctgggccgg gccggccggc gggcgatgga ggtggccggc atcacgccgg
     1381 ccgacctgga cgtggccgag atctacgact cgttcagctt cacgcccctg atcgccctcg
     1441 agggcttcgg cgtctgcggc cccggcgagg ccggggactg ggtgctggcc ggcaacgcca
     1501 agctgggcgg caagctgccc atgaacaccc acggcggcct gatccgccag gcccacctgg
     1561 gggccatgca ccacatcgtc gaggcggcac tgcagctgcg cggggaggcc gacgacagcg
     1621 atcgcggcgg cggcaaccac caggtgccgg gcgccaagct ggcgatcacg aacggctccg
     1681 gcgggatcct cgccgcgacg agcgcgctgg tcctgtcgag cgagccgccc tcggcggcct
     1741 agaggcacgg aagaggagac gacgatgccc gacgaggcga ccaagcccgc ctacaccaag
     1801 ccccttcccg acctgcgccc cgagggcgag cgcttctggg agggcacccg cgagcacaag
     1861 ctgctgctgc cgaccgacgc ccagggcaag cccttctggt acccgcgggc gctggtcccc
     1921 ggggcgctgg agccggtcgg ctgggtcgag gccgccggca ccggcgaggt ctacacctac
     1981 tcggtccact tcatcggccc gagcaaggcc tacaagggcg acccgcccca cgtcgtcgcg
     2041 ctcatcgacc tcgacgaggg cgtgcggatg atgtccaacc tggtccgcga cgagtcgccg
     2101 gactacccgt cgatcgaccc ggacgacgtg tcgatcggca tgcgcgtgcg cgtcgtcttc
     2161 cacgacgtca ccgacgagat caccctgccc aagttcgtcc ccgcctgaac ggaggcgccg
     2221 ggcacccacg acagcaccga gggggaaggc tgagcatggc cgagtggagc gactggtacg
     2281 cgcgcgagga cccgacgcag tgggtcctgg ccgagatcct caagcgccgg gcgagcgaga
     2341 ccccggaccg cgactacctc aagttcgccg agaacccctg ggtgagctac ggcgaggtca
     2401 acgcccgggc caaccgggtc gccaacgccc tgatcgaccg gggggtggag ccgggcgagt
     2461 cggtcagcgt gatgctgccc aactgcgagg agttcctgcc ggtctggtac ggcatcctca
     2521 aggccggggc ggtgatgagc tcgatcaaca ccgcctacaa gggcgacttc ctgtcctgga
     2581 cgatcaacct ggtcgaggcc aagaagctgg tcatctccga cctctacctg gaccggctcg
     2641 acctcatcaa gggcgagctg ccgaagctcg agcacgtgat cgtgatgcag accggcgccc
     2701 aggagggccc ggacccctcc atggcgtggg agccgctggc cgagctgatg gcggccaccg
     2761 acggcgagcc ggacggggtc agctactcct ggaccgacga cgcccgcatc atgttcacct
     2821 cgggcaccac cggccgctcc aagggcgtca tcaagcagaa cgccgccgac tacttctcgg
     2881 cccgcggcct gctggaggtg gtcagcgcga cggccggcaa gagcgtcgag tcgctggagg
     2941 aggacacctt cttctcctgc ctgccgctct tccactccaa cgcccaggtg ctctcgggct
     3001 acccggcgct ggtggccggc ggccgggtcg cctacgtgga gcgcttctcc tcgagcaggt
     3061 tctggcagca ggtgatcgac gccgacgcga cggtcttcaa cagcatcggg gcggtcagct
     3121 acttcatctg gaacatcccg gcctcggagc tcgaccgggc ccacacggtc cacacctgct
     3181 tcgccgcgcc ggcgcccaag gacatctaca acgagttcca ggagcgcttc ggggtcaagt
     3241 tcatcgaggg ctacgggctc accgagaccg gcatggccac ctacatggac ccgaccaagc
     3301 cggcggtgcc cggctcgatg ggtaaggcca acccgggcta cgaggtgagc atcgtcgagc
     3361 cggggaccga ccggcccctg ccgcccgaga ccccgggcga gatcgtggtc gacatgaaga
     3421 tccccaacat cgtcatgcgc gcctactacg gcatgcccga
//
LOCUS       DASTFV010000257         2743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_970282, whole genome shotgun sequence.
ACCESSION   DASTFV010000257 DASTFV010000000
VERSION     DASTFV010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970282"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFP02_04870"
     CDS             complement(<1..299)
                     /locus_tag="VFP02_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET9542386.1"
                     /translation="MKVCVVGGGGREHALAHVLGRSADVVVTPGNPGIPGSVTTPPEA
                     VEADLFVIGPEAPLVDGLADRLRAQGRLVFGPGADGARLEGSKAFMKELLASAGVP"
     gene            complement(310..1596)
                     /locus_tag="VFP02_04875"
     CDS             complement(310..1596)
                     /locus_tag="VFP02_04875"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET9542387.1"
                     /translation="MPATVVVGTQWGDEGKGKFTDLVAKEMQLVVRYQGGHNAGHTLV
                     VDGDSFALQLVPSGVLYDHITPIIGNGVVVDPRVLITELDMLESRGVDTSRLVLSGNA
                     HLILPYHQAIDVMIERHLGKNKLGTTKRGIGPAYADKAARIGIRVQDLLDPKIFREKL
                     DVALREKNPLLAKVYNQLGFDAEVIVEEYVGRCLPRLERHIGDAIGLVHQALDAGQHV
                     LLEGAQATFLDLDHGTYPFVTSSNPVAGGACTGAGVGPLHINRVIGIAKAYVTRVGSG
                     PFVTELPEGDPIGDLLVDLGHEYGTNTGRRRRPGWLDAVMLRHAVRLNSLSELALTKL
                     DIFDTLDTIKVCVAYDVDGERHEQLPYHQSELFHATPVYEELPGWREDLSEATELHDL
                     PAKAQEYLTFLEEQIGVPIRLVGVGPGRTQFVQRVP"
     gene            complement(1674..2303)
                     /locus_tag="VFP02_04880"
     CDS             complement(1674..2303)
                     /locus_tag="VFP02_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542388.1"
                     /translation="MKERGATLVEYALIISVFVFGSMGAIQRLDDKSGDYYENASNDI
                     GELPQTGIDPDEPGPTSSTTSTTTTTITTTTTTTTTTTTTTTTTTTTTTTTVPTTTTT
                     TIPTRSTITELTDSSTNNGTSFNAIARVRIRHNKTAAAINNATVTFLLTDSQGSTTTK
                     QCTTDSTGRCSTIAWSRSDSRSPVTARVTGVSASPTWDGVQASVLLRNP"
     gene            complement(2393..>2743)
                     /locus_tag="VFP02_04885"
     CDS             complement(2393..>2743)
                     /locus_tag="VFP02_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542389.1"
                     /translation="SHIVRLIVYKATTADSAVPATCLAVPGSGGVSGVCNVYVASDLA
                     RPLSDYGGTTSCSGTAPDRYWCPTTRQNSQALGADYLGVWMEIRYTYVTHVLPGTGIT
                     IRDRAVMRLEPRLT"
BASE COUNT          367 a          883 c         1038 g          455 t
ORIGIN      
        1 ggcaccccgg ccgacgccaa cagctccttc atgaacgcct tcgagccctc caggcgggcc
       61 ccgtcggcgc ccgggccgaa gacgagtcga ccctgggccc ggagccgatc ggccaggccg
      121 tcgaccaacg gggcctcggg gccaatgacg aacaggtcgg cctcgacggc ctcagggggc
      181 gtcgtgaccg acccggggat gcccgggttg cccggcgtga ccacgacgtc ggccgagcgg
      241 ccgaggacgt gggccagggc gtgctcgcga ccgccgccgc cgacgacgca gaccttcacg
      301 ccgccgggct cacgggacgc gctggacgaa ctgggtccgc ccggggccga cgccgaccag
      361 ccggatgggc acgccgatct gctcttccag gaacgtcagg tactcctggg ccttcgccgg
      421 caggtcgtgc agctcggtgg cctcgctcag gtcctcgcgc cagcccggga gctcctcgta
      481 gacgggcgtg gcgtggaaca gctcgctctg gtggtacggc agctgctcgt ggcgctcacc
      541 gtcgacgtcg taggccacac agaccttgat cgtgtccagg gtgtcgaaga tgtccagctt
      601 ggtgagggcc agttcggaca gggagttcag gcgcacggcg tggcggagca tgaccgcgtc
      661 cagccagccc ggccggcggc gacgaccggt gttggtcccg tactcgtggc cgaggtcgac
      721 cagcaggtcg ccgatggggt caccctcggg cagctcggtg acgaagggcc cggacccgac
      781 ccgggtgacg taggccttgg cgatcccgat gacccggttg atgtggaggg ggccgacgcc
      841 ggcgccggtg caggccccgc cggccaccgg gttggacgac gtcacgaagg gataggtgcc
      901 gtggtcgagg tccaggaagg tggcctgagc gccttccagt aggacgtgct ggccggcgtc
      961 gagggcctgg tggacgaggc cgatggcgtc gccgatgtgg cgctccagcc gggggaggca
     1021 ccgcccgacg tactcctcca cgatgacctc ggcgtcgaag ccgagctggt tgtagacctt
     1081 ggccaacagc gggttcttct cccgcagggc cacatcgagc ttctcgcgga agatcttggg
     1141 gtcgagcagg tcctggaccc ggatgccgat gcgggccgcc ttgtcggcgt acgccgggcc
     1201 gatccctcgc ttggtggtgc ccagcttgtt cttgcccagg tggcgctcga tcatcacgtc
     1261 gatggcctgg tggtagggca ggatcaggtg ggcgttgccc gagagcacga gccggctggt
     1321 gtccacgccg cgggactcga gcatgtccag ctccgtgatg aggacccggg ggtcgacgac
     1381 gacgccgttg ccgatgatcg gcgtgatgtg gtcgtagagg acacccgagg gcaccagctg
     1441 gagggcgaag ctgtcgccgt cgaccacgag cgtgtggccg gcgttgtgac cgccctggta
     1501 gcggacgacg agctgcatct ccttggcgac gagatcggtg aacttcccct tgccctcgtc
     1561 gccccactga gtgccgacga cgacggtcgc gggcacggtg tctcccctcg tcgggtccgc
     1621 gtgcggcgag agcctaccga gcggacccac cggccatcag cggccggtgc cgatcagggg
     1681 ttgcggagca ggaccgaggc ctgcacgccg tcccacgtcg ggctggcgct gacaccggtg
     1741 acccgggcgg tgacgggcga gcggctgtcg ctgcggctcc aggcgatggt gctgcagcgc
     1801 ccggtgctgt ccgtcgtgca ctgcttggtc gtggtcgacc cctggctgtc cgtgaggagg
     1861 aaggtgaccg tggcgttgtt gatggcggcg gcggtcttgt tgtggcggat ccggacccgg
     1921 gcgatggcgt tgaagctggt gccgttgttc gtcgaggagt cggtcagctc ggtgatcgtg
     1981 ctgcgggtcg ggatcgtggt ggtcgtcgtg gtcgggaccg tcgtggtcgt ggtggtcgtc
     2041 gtggtcgtcg tcgtcgtggt cgtggtggtc gtcgtcgtgg tcgtcgtggt gatggtcgtc
     2101 gtggtcgtcg aggtcgtcga ggacgtcggg ccgggttcgt cggggtcgat ccccgtctgc
     2161 gggagctcgc cgatgtcgtt gctggcgttc tcgtagtagt cgcccgactt gtcgtccagc
     2221 cgctggatgg cccccatgga gccgaagacg aagaccgaga tgatcagggc gtactcgacc
     2281 agcgtggcgc cgcgctcctt caccctggcc gtacccctac cccgcgtcca gtcacccatg
     2341 gacgggtcat cggcacgtcg gcggccctgc tggagaaatt cgtggcggcc catcaggtca
     2401 gcctcggctc gaggcgcatc actgcccggt cccggatcgt gatgccggta cccgggagca
     2461 cgtgggtcac gtacgtgtac cggatctcca tccagacacc gaggtagtcg gcgccgagag
     2521 cctggctgtt ctggcgtgtg gtcgggcacc agtagcggtc cggcgcggtg cccgagcagg
     2581 aggtggtgcc cccgtagtcc gacagggggc gagccaggtc cgaggccacg tacacgttgc
     2641 agacgccgct cacgccgccg ctgcccggca ccgccaggca ggtggcgggc accgccgagt
     2701 ccgccgtcgt cgccttgtag acgatgagcc ggacgatgtg gga
//
LOCUS       DASTFV010000258         3411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_971606, whole genome shotgun sequence.
ACCESSION   DASTFV010000258 DASTFV010000000
VERSION     DASTFV010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971606"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFP02_04890"
     rRNA            complement(<1..546)
                     /locus_tag="VFP02_04890"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(933..2219)
                     /gene="tyrS"
                     /locus_tag="VFP02_04895"
     CDS             complement(933..2219)
                     /gene="tyrS"
                     /locus_tag="VFP02_04895"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET9542390.1"
                     /translation="MTGSAHVLDDLEARGLVQDHTDRDALHEALGAGPVVLYAGFDPT
                     ADSLHLGNLVPLLVLRRFQDFGHRPIVLAGGATGMVGDPGGRSDERNLLDPEALRANT
                     AAINVQLAQFLAFDGEAAAILVDNRDWTEGVTLLDFLRDVGKHVTVNQMTAKDSVRNR
                     LESETGISFTEFSYMLLQAHDYWWLYTNHDCVLQLGGSDQWGNITAGIDLIRRKEAAT
                     VHGLTVPLMTRADGAKFGKSQDGSVWLDPAKTTPYQFFQYLVQMGDRDAERFLLQLTL
                     LPVERCAEIVAEHQRAPERRLAQRELARALTTLVHGLAASSGAEQASAVLFGGPVDGL
                     DEAGFEVLAAEVPTVDLEPGELGGGVELVDAMVRGGLATSKGDARRTIEQGGVYVAGA
                     QTRDVERKLSGGDAVGGRYVLLRRGKANHLLLREAG"
     gene            2304..2807
                     /locus_tag="VFP02_04900"
     CDS             2304..2807
                     /locus_tag="VFP02_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542391.1"
                     /translation="MARSRPRVGTVLAVVVVAATIGMWAYLFLIADPGVPDELEDDTF
                     PTEGQAICADAVARIDELPAAQEATSPEDRAVSVAAANEILTEMVTDLRAIAPTDGQD
                     ERLTRLWLEDWDSYLADRADYADQLAAGEDAELMITARGAGQITVTLDHFAVVNHMLD
                     CQTPLDA"
     gene            complement(2819..3073)
                     /locus_tag="VFP02_04905"
     CDS             complement(2819..3073)
                     /locus_tag="VFP02_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET9542392.1"
                     /translation="MVFWLGVATTAVSYVLFSAGLQRLRSSTVATLSLTEPLTAALLG
                     VVVLGERPGLLALLGALVLLSGLLVISTGPVPIDEPLPEP"
     gene            complement(3094..>3411)
                     /locus_tag="VFP02_04910"
     CDS             complement(3094..>3411)
                     /locus_tag="VFP02_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542393.1"
                     /translation="PHRHRGGRRGAMALVRPRRPQPVPVPLMDRFRPELLIVAAAVLW
                     GTTGTAQDLGDVQASPVVVGVAKIALGSALLALLALLALLALLARSGTWNARWATNDR
                     FQT"
BASE COUNT          519 a         1170 c         1184 g          538 t
ORIGIN      
        1 caataaatcc ggacaacgct cgccccctac gtgttaccgc ggctgctggc acgtagttgg
       61 ccggggcttc ttctggaggt accgtcattt tcgtccctcc tgaaaggggt ttacaacccg
      121 agagccttca tcccccacgc ggcgttgctg cgtcaggctt tcgcccattg cgcaagattc
      181 cccactgctg cctcccgtag gagtctgggc cgtgtctcag tcccagtgtg gctgatcgtc
      241 ctctcagacc agctacccgt cttagccttg gtgagccgtt acctcaccaa ccagctgata
      301 ggccgcgaga ccctcccgga gcgtcggagc atttcctcac caaatcatgc ggttcggtgg
      361 gggtatccgg tattagctcg agtttcctcg agttatcccg gtctccgggg caggtttctc
      421 acgtgttact cacccgttcg ccgctagatc ttcaccagtg ttgccactgg atggatctcg
      481 ctcgacttgc atgtgttagg cacgccgcca gcgttcgtcc tgagccagga tcaaactctc
      541 cgtcaagatc tacggccggc cacccgaagg tgatcgtccg atcgatcggt ttccgaagag
      601 ccggtcgtct gctgcagcac agcttcggac cgacccgact ggattgttct ttgctcccgt
      661 tccttcaggg cgaacccttc ggggaggcgg gagcggaatt gatgtgatga acgatcgtgg
      721 ttccacggcc tccggacgcg aacgtcccga ggtcatgtcc cgacgatcgc ccgcactggc
      781 atatagatcg tcttctgttc cgttttcaag gagcgccgga ggctcgtccg cgccagtggc
      841 gagagatcga gccggatgct ctgagcttcg cgcaccctgc gggcagggcc gtccgaagcg
      901 acttgcgaat ctaccgtggc tcggtcagaa cgtcaacccg cttcccggag gagcaggtgg
      961 ttcgccttac cgcggcggag cagaacgtat cgccccccca ccgcgtctcc acccgaaagt
     1021 ttccgctcga cgtcccgggt ctgcgcgccc gccacgtaca cgccgccctg ctcgatggtg
     1081 cggcgggcgt cccccttcga cgtggccaga cccccgcgca ccatcgcgtc gaccagctcg
     1141 acgcccccgc ccagctctcc gggctcgagg tcgacggtcg gcacctcggc ggccaggacc
     1201 tcgaagccgg cctcgtcgag gccgtcgacc gggccaccga agagcacggc ggaggcctgc
     1261 tcggcgccgg acgaggcggc caggccgtgg accagggtcg tcagggcccg ggccagctcc
     1321 cgctgggcca ggcggcgttc cggggcccgc tggtgctcgg ccacgatctc ggcacaccgc
     1381 tcgacgggga gcaaggtgag ctggagcagg aagcgctcgg cgtcccggtc gcccatctgc
     1441 acgaggtact ggaagaactg gtacggcgtc gtcttggccg ggtcgagcca gacgctgccg
     1501 tcctggctct tgccgaactt ggccccgtcc gcccgggtca tcaggggcac cgtcaggccg
     1561 tggacggtcg ccgcctcctt gcgccggatc aggtcgatgc cggcggtgat gttgccccac
     1621 tggtcggagc cgccgagctg gaggacacag tcgtggttgg tgtacagcca ccagtagtcg
     1681 tgggcctgga ggagcatgta gctgaactcg gtgaaggaga tgcccgtctc gctctcgagg
     1741 cggttgcgca ccgagtcctt ggccgtcatc tggttgacgg tcacgtgctt gcccacgtcc
     1801 cgcaggaagt cgagcagggt cacgccctcg gtccagtccc ggttgtcgac caggatcgcg
     1861 gcggcctcgc cgtcgaaggc caggaactgg gccagctgga cgttgatggc cgccgtgttc
     1921 gcccgcaggg cctcggggtc gagcaggttg cgctcgtcgg agcggccacc ggggtccccc
     1981 accatgcccg tggcgccgcc cgccaggacg atcgggcggt gaccgaagtc ctggaagcga
     2041 cgcaggacca ggaggggcac gaggttgccg agatggagcg agtccgccgt ggggtcgaag
     2101 ccggcgtaga ggacgaccgg cccggccccg agcgcctcgt ggagggcgtc gcgatcggtg
     2161 tggtcctgga cgaggccgcg ggcctcgagg tcgtcgagca cgtgggcgct gccggtcacg
     2221 gcccaactct gccgcaccgg gcccggcccg acccagccat tggggattgg acaccccgag
     2281 gaccgggcgc ccacgctgga cggatggccc gtagccgtcc ccgcgtgggc accgtcctcg
     2341 ccgtcgtggt cgtggccgcc accatcggca tgtgggccta cctgttcctg atcgccgacc
     2401 ccggcgtccc cgacgagttg gaggacgaca ccttcccgac cgagggtcaa gccatctgcg
     2461 ccgacgccgt cgcccggatc gacgagctcc ccgcggccca ggaggcgacc tcgcccgagg
     2521 accgggccgt gtcggtggcc gccgccaacg agatcctcac cgagatggtc accgacctgc
     2581 gggccatcgc ccccaccgat ggccaggacg agcgcctcac ccgcctctgg ctggaggact
     2641 gggacagcta cctggccgac cgggccgact acgccgacca gctggcagcc ggcgaggacg
     2701 ccgagctcat gatcaccgcc cgcggcgccg gccagatcac cgtcaccctc gaccacttcg
     2761 ccgtggtcaa ccacatgctc gactgccaga cccccctgga cgcgtagggc gtgatcgttc
     2821 agggctcggg caggggctcg tcgatgggta cgggtccggt gctgatgacg aggaggccgg
     2881 agagcagtac cagggcgccc aggagggcca ggaggcctgg gcgctcgccg aggaccacca
     2941 cgcccagcag cgcggccgtg agcggctcgg tgaggctcag ggtggcgacg gtcgaggacc
     3001 gcagccgctg gaggccggcg ctgaagagga cgtaggacac ggctgtcgtg gccacgccca
     3061 gccagaagac catcgcgatg cccttgccgg tcgtcaggtc tggaacctgt cgttggtggc
     3121 ccaccgcgcg ttccaggttc cagaacgggc gaggagggcg aggagggcga ggagggcgag
     3181 gagggcgagg agggcactgc cgagggcgat cttggccacg ccgacgacga ccggggaggc
     3241 ctggacgtcg ccgaggtcct gggcggtgcc cgtcgtgccc cagaggacgg cggccgcgac
     3301 gatcagcagc tcggggcgga agcggtccat cagcgggacc gggacaggtt ggggtcgccg
     3361 gggacgaacc agcgccatgg cgcctcgacg gccaccgcga tgccgatgcg g
//
LOCUS       DASTFV010000259         5042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_972443, whole genome shotgun sequence.
ACCESSION   DASTFV010000259 DASTFV010000000
VERSION     DASTFV010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972443"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..2402)
                     /locus_tag="VFP02_04915"
     CDS             complement(114..2402)
                     /locus_tag="VFP02_04915"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9542394.1"
                     /translation="MGATTHLTLSGLRHRGWAQVLALVGVCALAATAVVAGLAAQTTA
                     ADQVDAAYQRAGRPDLVLYGEAPALEAAADDDAVAEASGPRAYVADLTTPVDGDAIDV
                     RLIATDPEALPAVATPDLVAGHWPEAGDEDAVVVEQSLVSEGVAAVGDTLTVESPTGP
                     TDLHVVGAAVDLLDCFWPTCDPLRLFARPETVDALAPDSQPSSFLAAYALRDPASAAT
                     VGGRLVADPAADIHGANHWTDTRADILVVGSVFATLVGGFGVFLLVAAAFVVAGATAA
                     RLVARRRSLGLLAATGFTPRQLLAAVWAEHLLLGAVGVLLGWVVGTPLAPLFQAGLDG
                     VLAGSGPGFALRSLLVAALLVGGLLTVSVLVPAGRAVRQSPSAVLRDVPTVPDGGRRL
                     AALARRAGAGPATASGLRRAFARPGRTALAAGALVVAAAGAVVSLGFIGTIDAVAADP
                     AVAGNPWDVTVESQTAAAGEVAEVLDTTEEVGAWFTERERNGTVDDAGFTVRVLGGDP
                     TAAAYVIQEGAPLRAADEAIVGYAFLDETGLAVGDPLRVEVDDTVLDLTIVGWYSDTA
                     DTGKMIQVREEALPAGLLDDDPDWRVVAAPGVSDEDLAAVLNDRFAGTAVAETLSVDD
                     LTPVKGAMVAMAVLLGAVALANLLATTLSATRERARAIGILRTVGASTGQLLTQAAVG
                     AGAIGGLATLVGIPLGWVAYTALADGLTTGIGIGPGFASDPSRVAVALVLPAVALLAA
                     GTGALASLGLARRPASALVRYE"
     gene            complement(2402..3142)
                     /locus_tag="VFP02_04920"
     CDS             complement(2402..3142)
                     /locus_tag="VFP02_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9542395.1"
                     /translation="MTTTEPLLVAHAVSKSFATATRRVDAVRQVDLQVDAGEWVAIMG
                     PSGCGKSTLLHLLGGLDVPDEGSVVLAGTSLGERSETARAVLRRSRVGYVFQFYNLVP
                     DLDALENVEIAAQLAGAGRREARRRARALMDELGLGEVIGAAVNELSGGEQQRVALAR
                     ALVNDPPLLLADEPTGALDTESARGVLGLLRQAHERGRTIVMVTHDHRVAAAADRVLV
                     MQDGQIRDERSLASSGRPLLAELLSLES"
     gene            complement(3139..3693)
                     /locus_tag="VFP02_04925"
     CDS             complement(3139..3693)
                     /locus_tag="VFP02_04925"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9542396.1"
                     /translation="MKHVFLALLASGRTHGYELKRRYDQLFAAVWGPVNIGQIYVTLG
                     RLERDGLVVQERVGQDGRPDRKDYELTERGRSALDAWLREDDALPVPKSDLVLKLVGA
                     SLLGTDDARALIGEHRQRCFQSLRDLDTATADTPGDSVAGVLSHGTALHLQAELQWLD
                     HCDEVLARGPLRLEPQLIPGDNPR"
     gene            3796..>5042
                     /locus_tag="VFP02_04930"
     CDS             3796..>5042
                     /locus_tag="VFP02_04930"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9542397.1"
                     /translation="MRFDVAPDPLTADDATIRAAVEGADVAPLLVAVAQLTGDHDLLV
                     EDLRPDQTRLLEPEAGITPEEMARGRDLAAAALARYRDAGSVPAPTPEGEDLRRLIDY
                     LVGADTDEYLPMLTEELALDGDKRAPAWTKADVAPDTAFRVAIIGAGMSGIATAHRLQ
                     QAGVPFTIFEKNPDAGGTWLENVYPGCRVDVPNHVYSYSFAQTGDWPQYYSTQDVLLD
                     YFQACLDRFGLREHVRFGTEVAGATWDEDAQTWTLQIVGGEGGDREEPGYQALVSAVG
                     QLNRPHWPNIDGIERFAGQSFHSAEWDHGIDLRGKRVAIIGTGASAAQFIPAVADQAA
                     ELAVFQRTAPWLVPTPTYHDELHEGLRWVLDHVPGYARWDRLWLFWRSLEGLGPMAVV
                     DPDWDEGELSVSLPNDLIRQLLTA"
BASE COUNT          695 a         1841 c         1890 g          616 t
ORIGIN      
        1 gaggccgtgc ccggggcccg cttcgagctc atcgagggcc tcggccacga ttacccgccg
       61 gcctactggg acacgatcgt gagcctcgtc gccgagcacg cccaccgcgc cgatcactcg
      121 tagcgaacga gcgccgaggc cggccggcgg gcgaggccga ggctggccag cgccccggtg
      181 ccggcggcga ggagggcgac ggcgggcagg accagggcca cggccaccct cgacgggtcc
      241 gaggcgaagc ccggcccgat gccgatcccg gtggtcaggc cgtcagccag cgcggtgtag
      301 gccacccagc cgagagggat gccgacgagc gtggccagcc cgccgatggc tcccgcccct
      361 accgccgcct gggtgaggag ctggccggtg ctggccccga ccgtgcgcag gatcccgatg
      421 gcccgggccc gttcgcgggt ggcgctcagc gtggtggcca gcaggttggc cagggccacg
      481 gcgcccagca gcacggccat ggccaccatc gcccctttga ccggggtcag gtcgtcgacg
      541 ctgagggtct cggcgacggc ggtccccgcg aaccggtcat tcaggacggc ggccaggtcc
      601 tcgtcgctca ccccgggggc ggcgaccacg cgccagtccg ggtcgtcgtc gagcaggccg
      661 gccggcaggg cctcctcccg gacctggatc atcttcccgg tgtcggcggt gtcggagtac
      721 cagcccacga tcgtcaggtc gaggaccgtg tcgtcgacct cgacccggag ggggtcgccg
      781 acggccagtc ccgtctcgtc gaggaaggcg tagcccacga tggcctcgtc ggccgctcgc
      841 agcggtgccc cctcctggat gacgtacgcg gcggccgtcg ggtcaccgcc gaggacgcgg
      901 accgtgaagc cggcgtcgtc caccgtgccg ttgcgctctc gttcggtgaa ccaggccccg
      961 acctcctcgg tggtgtccag gacctcggcc acctcgcctg cggcggcggt ctggctctcg
     1021 acggtcacgt cccacgggtt gccggccacc gcgggatcgg cggccacggc gtcgatggtg
     1081 ccgatgaacc cgaggctgac cacggccccg gcggcggcga ccaccagggc gcccgcggcc
     1141 agggccgtcc gtcccggccg ggcgaacgcc cggcgcaatc cactggccgt cgccggaccg
     1201 gcgccggccc ggcgggccag agcggccaga cgccgacccc cgtcgggaac ggtgggcacg
     1261 tcgcggagca cggcgctggg ggactggcgc accgcccgcc cggccggcac caacacgctc
     1321 acggtgagca ggccgccgac cagcagtgcg gccaccagca gcgagcgcag ggcgaagccg
     1381 gggcccgacc cggccaacac gccgtcgagg ccggcttgga agaggggggc cagcggcgtc
     1441 cccaccaccc agcccaggag cacgccgacg gcgccgagca ggaggtgctc ggcccacacc
     1501 gcggccaaga gctggcgggg ggtgaagccg gtggcggcga gcaggccgag ggaccggcgc
     1561 cgggcgacca ggcgggccgc agtggccccg gcgaccacga aggcggccgc gaccaggagg
     1621 aagacgccga agccaccgac cagggtggcg aacacggaac cgacgacgag gatgtcggcc
     1681 cgggtgtcgg tccagtggtt ggccccgtgg atgtcggcgg ccggatcggc cacgaggcga
     1741 cctccgaccg tggccgccga cgccggatca cgaagggcgt aggcggcgag gaagctcgac
     1801 ggctggctgt cgggggccag ggcgtcgacc gtctcgggcc gggcgaacag gcgcagcggg
     1861 tcacaggtgg gccagaagca gtcgagcagg tcgacggcgg cgcccaccac atggaggtcc
     1921 gtgggcccgg tcgggctttc gacggtgagg gtgtcaccca cggcggcgac gccctccgag
     1981 accaacgact gctcgacgac cacggcgtcc tcgtcgccgg cctcgggcca gtgacccgcc
     2041 accaggtccg gagtggccac cgcgggcagt gcttcggggt cggtggcgat gaggcggaca
     2101 tcgatggcgt cgccgtcgac cggggtcgtg aggtcggcga cgtaggcccg gggcccggat
     2161 gcctcggcca ccgcatcgtc gtcggctgcg gcctcgaggg caggggcctc gccgtagagg
     2221 acgagatcgg gccggcccgc ccgctggtag gcggcatcga cctggtcggc cgcggtggtc
     2281 tgcgcggcga ggccggccac gaccgccgtg gcggcgaggg cgcagacgcc gaccagggcc
     2341 aggacctgcg cccagccccg gtgccggagc ccgctgaggg tgaggtgggt ggtggcgccc
     2401 atcacgactc cagggagagg agctcggcca ggagggggcg gccgctggag gccagactgc
     2461 gctcgtcccg gatctggccg tcctgcatga ccaggacccg gtcggcggcc gccgccaccc
     2521 ggtggtcgtg ggtgaccatc acgatcgtcc gtccccgctc gtgggcctgg cggaggaggc
     2581 ccaggacgcc ccgggccgac tcggtgtcca gtgccccggt cggctcgtcg gccaggagga
     2641 gcgggggatc gttgaccagc gcccgggcga gggcgacccg ttgctgctcg ccgccggaca
     2701 actcgttcac cgcggcgccg atcacctccc ccaggccgag ctcgtccatc agggcgcggg
     2761 ctcggcggcg cgcctcccgc cggcccgccc cggccagctg ggccgcgatc tcgacgttct
     2821 ccaacgcgtc caggtcgggc accaggttgt agaactggaa gacgtagccc acccgcgagc
     2881 ggcggagcac ggcccgggcc gtctcggacc gctcgccgag ggaggtgccg gccagtacga
     2941 ccgagccctc gtcgggcacg tccaggccgc ccagcaggtg cagcagggtg gacttgccgc
     3001 aaccgctggg gcccatgatc gccacccatt cgccggcgtc gacctgcagg tcgacctggc
     3061 gcacggcgtc cacccggcgg gtggcggtgg cgaaggactt gctcacggcg tgggccacga
     3121 gcaggggttc ggtggtggtc atcgtgggtt gtctcctggg atcagttgcg gttcgaggcg
     3181 aagggggcca cgggccagga cctcgtcgca gtggtccagc cactgcaact cggcctgcag
     3241 gtgcagcgcc gtcccgtggg agaggacccc ggcgacggag tcaccggggg tgtcggcggt
     3301 ggccgtgtcg aggtcgcgca ggctctggaa gcaccgctgc cggtgctcgc cgatgagggc
     3361 ccgagcgtca tcggtgccga gcagggaggc gccgaccagc ttgagcacca ggtccgactt
     3421 gggcaccggg agggcgtcgt cctcccgcag ccacgcgtcc agggcggacc ggccccgctc
     3481 ggtcagctcg tagtccttgc ggtccggtcg gccgtcctgg ccgacccgct cctggaccac
     3541 gaggccgtcc cgctcgaggc gacccagcgt gacgtagatc tggccgatgt tgaccgggcc
     3601 ccagacggcg gcgaacagct ggtcgtagcg ccgcttcagc tcgtagccgt gggtgcggcc
     3661 cgacgccagg agggcgagga agacgtgctt cacccctgtc cgctcctatg cataggcgtt
     3721 atgtataaca agtatctagc tggtgtcaag cccccgttgc gcccggcctc tgcccgcgtg
     3781 cgaggctgga ggggcatgcg cttcgacgtc gctcccgacc ccctcaccgc cgatgacgcc
     3841 acgatccggg ccgccgtcga gggggcggac gtggcccccc tgctcgtcgc cgtcgcccag
     3901 ttgaccggtg accacgacct gctggtcgag gacctgcgcc cggatcagac ccgcctgctc
     3961 gagcccgagg cgggcatcac ccccgaggag atggcgcggg gccgggacct ggccgccgcc
     4021 gcgctggccc ggtaccgcga cgcggggtcg gtgccggcgc cgacccccga gggcgaggac
     4081 ctgcgccgcc tgatcgacta cctcgtgggc gccgacaccg acgagtacct gccgatgctg
     4141 accgaggaac tggccctcga cggcgacaag cgggctccgg cgtggaccaa ggcggacgtc
     4201 gccccggaca ccgctttccg ggtggccatc atcggggcgg gcatgtccgg catcgccacc
     4261 gcccaccggc tgcagcaggc gggggtgccg ttcaccatct tcgagaagaa ccccgacgcc
     4321 gggggtacct ggttggagaa cgtctacccc ggctgccggg tggatgtccc caatcacgtc
     4381 tacagctact cgttcgccca gaccggcgac tggccgcagt actactccac ccaggacgtg
     4441 ctcctggact acttccaggc ctgcctcgac cggttcgggc tccgggagca cgtccgcttc
     4501 ggcaccgagg tggccggggc cacctgggac gaggacgccc aaacctggac cctgcagatc
     4561 gtcggcgggg aaggcggcga ccgggaggag cccggctacc aggcgctggt cagcgccgtg
     4621 ggccagctca accgacccca ctggccgaac atcgacggca tcgagcggtt cgcggggcag
     4681 tccttccact ccgccgagtg ggaccacggc atcgatctcc gcggcaagcg ggtggccatc
     4741 atcggcaccg gcgccagcgc cgcccagttc atccccgccg tggccgatca ggccgccgag
     4801 ctggccgtgt tccagcgcac cgccccctgg ctggtgccca cgccgaccta ccacgacgag
     4861 ctccacgagg gcctgcgctg ggtcctcgac catgtcccgg gctacgcccg ctgggaccgg
     4921 ctgtggctct tctggcggag cctggagggc ctgggcccca tggcggtggt cgacccggac
     4981 tgggacgagg gcgagctgtc ggtcagcctg cccaacgacc tcatccgcca gctcctcacc
     5041 gc
//
LOCUS       DASTFV010000260         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_973706, whole genome shotgun sequence.
ACCESSION   DASTFV010000260 DASTFV010000000
VERSION     DASTFV010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973706"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VFP02_04935"
     CDS             <1..457
                     /locus_tag="VFP02_04935"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET9542398.1"
                     /translation="FRLRKFRTMTEDRDAATGTLRPDDERLTPFGRRLRATSLDELPE
                     LWNVVAGDMAFVGPRPLPVAYRDRFTSEEHRRHEVRPGITGWAQVHGRNRLDWDDRLA
                     LDVWYVDHRSLRLDLRILGRTVGAVLRRDGIAGSGTATMADLPADRADV"
     gene            486..878
                     /locus_tag="VFP02_04940"
     CDS             486..878
                     /locus_tag="VFP02_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542399.1"
                     /translation="MDTEEEVVRRLEAEGYVHHFVPDAEGLRCPECETVADPRMVTVD
                     ETVRFEGSSSPGDESVVFAIANGPCGHRGTLVAAYGPNASPDEAEVVRTLGQEPADDP
                     ITAREGAELDLMEADASEAGAELGDEMP"
     gene            complement(882..1244)
                     /locus_tag="VFP02_04945"
     CDS             complement(882..1244)
                     /locus_tag="VFP02_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HET9542400.1"
                     /translation="MTSGHTVTIAPGTNHVEVRVDGEKVAESDRPLLLEETGLPTRYY
                     LPREDVDLERFRKTSFHTTCPFKGEASYWSIEIGGTTHDGIVWSYEDPIPGAEAITDH
                     LSFYPDRAEVTVDGATLS"
     gene            complement(1286..>2039)
                     /locus_tag="VFP02_04950"
     CDS             complement(1286..>2039)
                     /locus_tag="VFP02_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HET9542401.1"
                     /translation="TTTGATPGWNNATRATEVAGPWQSARVLAVRDEAPRVKTFRLAL
                     DEPTGHRAGQHYVVRLTAPDGYTASRSYSVASAPDGTGEFELTVERLEDGEVSGYLHD
                     VVEPGDELEVRGPIGGWFVWEDGPALLVGGGSGVVPLMAMLRLARATDRSHLVRLVAS
                     VRTPTDLYYAEELPGPEVTVIHSRIAPPGSTRPVGRIQADDLAPAAPLVAEGATAYVC
                     GSTGFADAASTLLVDVGWPVERIRVERFGATG"
BASE COUNT          285 a          722 c          752 g          280 t
ORIGIN      
        1 gttccggctt cgcaagttcc gcaccatgac cgaggaccgc gacgcggcca cgggcacgct
       61 ccggcccgac gacgagcgcc tcacgccgtt cggccgccgg ctacgggcca cgagcctcga
      121 cgagctcccc gagctctgga acgtcgtcgc cggtgacatg gccttcgtcg gcccccgccc
      181 gctgcccgtg gcgtatcgcg atcgcttcac gtccgaggag caccgacgcc atgaggtccg
      241 gcccggcatc accggctggg cgcaggtcca cgggcgcaac cgcttggact gggacgatcg
      301 gcttgccctc gacgtctggt acgtcgatca ccggtccctg cgcctcgacc tgcgcatcct
      361 gggccggacc gtcggcgccg tgctgcgccg ggacgggatc gcgggctcgg gaacggcgac
      421 catggccgac ctccccgcgg accgggctga cgtctagggc tgatctcacg gggaagatca
      481 cggccatgga cactgaggaa gaggtcgtgc gtcggctgga ggcggagggc tacgtgcacc
      541 acttcgtgcc tgacgccgag ggcctgcgct gccccgaatg cgagaccgtg gccgaccccc
      601 gcatggtgac ggtggacgaa acggtgcggt tcgaggggtc gtccagcccg ggcgacgagt
      661 ccgtcgtctt cgccatcgcc aacggaccct gtggccaccg gggcaccctc gtcgccgcct
      721 acgggccaaa cgcctcgccg gacgaggccg aggtcgtccg cacgctcggc caggagcccg
      781 ccgacgaccc catcaccgcc cgcgagggcg ccgagctgga cctgatggag gccgacgcct
      841 ccgaggccgg cgccgagctg ggcgacgaga tgccctgagg gtcagctcag ggtcgcgccg
      901 tcgacggtga cctcggcccg gtccgggtag aacgacaggt ggtcggtgat ggcctcggcg
      961 ccggggatcg ggtcctcgta gctccacacg atcccgtcgt gggtcgtgcc cccgatctcg
     1021 atggaccagt aggaggcctc gcccttgaac gggcaggtgg tgtggaacga cgtcttccgg
     1081 aagcgctcga ggtccacgtc ctcccggggc aggtagtagc gagtgggcag gccggtctcc
     1141 tcgaggagca gcgggcggtc gctctcggcg accttctcgc cgtccacgcg gacctccacg
     1201 tggttcgtgc cgggggcgat cgtgacggtg tggccagagg tcatggacta cgtgcaacgg
     1261 tcgggagcct ccgaggattc ccgcttcagc cggtggcgcc gaagcgctcg acccggatgc
     1321 gttcgaccgg ccagccgacg tcgaccagca gcgtgctggc ggcgtcggcg aagcccgtcg
     1381 agccgcagac gtaagcagtg gcgccctcgg ccacgagagg ggcagcgggc gcgaggtcgt
     1441 cggcctggat ccggccgacc ggacgggtgc tgccgggtgg ggcgatccgg ctgtggatca
     1501 cggtgacctc ggggccgggg agctcctcgg cgtagtagag gtccgtcggc gtccgcaccg
     1561 acgccaccag ccgcaccagg tgggaccgat ccgtagcccg ggccaggcgg agcatggcca
     1621 tcagcgggac cacacccgag ccgccgccga ccagcagggc gggaccgtcc tcccagacga
     1681 accagccgcc gatggggccc cggacctcga gctcgtcgcc cggttcgacg acgtcgtgca
     1741 ggtagcccga gacctcgccg tcctccaggc gctcgacggt cagctcgaac tcgccggtcc
     1801 cgtcgggggc ggaggccacc gagtacgagc gggatgcggt gtagccgtcc ggggcggtga
     1861 ggcggaccac gtaatgctgg cccgcccggt ggcccgtcgg ttcgtcgagg gcgaggcgga
     1921 aggtcttgac cctcggcgcc tcgtcccgta cggccaggac ccgggcgctc tgccaggggc
     1981 cggccacctc agtcgccctg gtagcgttgt tccaaccagg ggtcgccccg gtcgtggta
//
LOCUS       DASTFV010000261         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_975441, whole genome shotgun sequence.
ACCESSION   DASTFV010000261 DASTFV010000000
VERSION     DASTFV010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975441"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="VFP02_04955"
     CDS             complement(<1..1310)
                     /locus_tag="VFP02_04955"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9542402.1"
                     /translation="MSSGEHDLVVRGGTVVDGTGAERRAADVAVDDGRITVVGDVAGG
                     GREELDATGLLVTPGWVDVHTHYDGQVTWDPLLTPSSWQGVTTVVMGNCGVGFAPVKP
                     DRHDWLIALMEGVEDIPGTALAEGISWGWESFPEYLDVLDGLPHAMDVAAQVPHGALR
                     GYVMGDRGAEHTEVPTADEIETMGRLAADGVRAGALGFTTSRTVNHRSSDGRPTPSLT
                     ATADELLGIARGVGATGMGVLQLVADLVDLDDEFALVRAMAEVSGRPLSITTLSEVHR
                     GDWRALLAKITEAQADGVPIRGQVASRPVGVLLGLEGRLHPLVASPTYQAMAALELGE
                     RVARLRAPEVRATVLGELTNGGLVFGSYDGIFPLGDPPEHDPGAERSIEAVATRAGRE
                     AVDVALDIMLEREGRGLLYLPFANYADRDFATIREMLLHPHTVPG"
     gene            complement(1310..1579)
                     /locus_tag="VFP02_04960"
     CDS             complement(1310..1579)
                     /locus_tag="VFP02_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542403.1"
                     /translation="MSSYDGKASLVFSSGDSFAECHLSATGTEGGWDGTLSDILFRTQ
                     MVPTPLNPTEHLRLEDVGGQPIVVVTVGDQDGESAQISGEGDPFS"
     gene            1661..2701
                     /locus_tag="VFP02_04965"
     CDS             1661..2701
                     /locus_tag="VFP02_04965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET9542404.1"
                     /translation="MKLDAMAMGLPLREAQRLAADIEEAGFSGLWFTEGGRTAYLGAT
                     AAALATSELDLGTGIAVAFPRSPMITASVAWELAEATGGRFVLGLGTQVKAHIERRYG
                     VTYEHPGPWLREYVLALRAIFRAFQGEERLDFHGEHYDLDLLPPTWSPGPIEHPDVPI
                     YLSGVRPWMLGATGEVADGLHVHPLHSRRYLDEVVVPNVAKGLATADRPVADVTLACP
                     VLTIMGDTDEEQDRWREAARFQLAFYGSTRTYRGVFETHGWDGTSERLHEHQKAGDLA
                     AMAAVITDEMLDAFAVTATWDGLAPALLDRYVGVEHPVRVICYFAGNSIRSDQTFDQW
                     AAVAAAVRAAAS"
     gene            complement(2717..>2988)
                     /locus_tag="VFP02_04970"
     CDS             complement(2717..>2988)
                     /locus_tag="VFP02_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542405.1"
                     /translation="DGPAVLERALAVAQDIAVNVAPRSVAASKAILWRSPAPSADEVD
                     AWERAVHVALMGSPDTTEGVMAWVEKRDPHWQGTLADGWPRDVVP"
BASE COUNT          401 a         1134 c         1043 g          410 t
ORIGIN      
        1 cccgggacgg tgtggggatg caggagcatc tcccggatgg tggcgaagtc ccggtcggcg
       61 tagttggcga acggcaggta gagcaggccc cgaccctccc gctccaacat gatgtcgagg
      121 gccacgtcga ccgcctcccg acccgcccgg gtggcgacgg cctcgatgct gcgctcggcg
      181 ccggggtcgt gctccggcgg gtcacccagc gggaagatcc cgtcgtagga cccgaagacc
      241 aggccgccgt tcgtcagctc gccgagcacc gtggcccgga cctccggggc gcgcagccgg
      301 gccacccgct cgcccagctc cagcgccgcc atcgcctggt aggtcggtga ggcgaccagc
      361 gggtgcaggc ggccctcgag gcccaggagc acgccgaccg gacgggaggc gacctgaccc
      421 cgaatgggca cgccgtccgc ctgggcctcg gtgatcttgg ccagcagggc ccgccagtcg
      481 ccccggtgga cctcggacaa ggtggtgatg gacagtggtc ggccgctgac ctcagccata
      541 gcccggacca gcgcgaactc gtcgtccagg tcgacgaggt cagccaccag ctggaggacg
      601 cccatcccgg tggcgcccac acccctggcg atgcccagca gctcgtcggc cgtggccgtc
      661 aggctcggcg tcggccgccc gtcgctcgaa cggtggttga ccgtccggga ggtcgtgaag
      721 ccgagggccc cggctcggac tccgtcggcc gccagccggc ccatcgtctc gatctcgtcg
      781 gccgtcggga cttcggtgtg ctcggcgccg cggtcgccca tcacgtagcc gcggagtgcg
      841 ccgtgcggga cctgcgcggc cacgtccatg gcgtggggca gtccgtcgag cacgtcgagg
      901 tactccggga agctctccca cccccagctg atgccctcgg ccagcgccgt gcccgggatg
      961 tcctccacgc cctccatcag ggcgatcaac cagtcgtgac ggtcgggctt caccggcgcg
     1021 aacccgacgc cacagttgcc catgaccacg gtcgtgacgc cctgccacga agacggggtc
     1081 agcagcgggt cccaggtgac ctgaccgtcg tagtgggtgt ggacgtcgac ccagccgggc
     1141 gtgaccagca gccccgtggc gtccagctcc tcccggcccc caccggcgac gtcaccgacg
     1201 acggtgatcc gcccgtcgtc gacggccacg tcggccgccc gccgttccgc tccggtcccg
     1261 tcgacgaccg tcccgcctcg gacgaccagg tcgtgctccc ccgaggacat cacgagaacg
     1321 ggtcgccctc gccggagatc tgggcggact cgccgtcctg gtcgcccacg gtgaccacga
     1381 cgatcggctg gccgccgacg tcctccaggc gcaggtgctc ggtggggttc agcggcgtgg
     1441 gcaccatctg ggtccggaag aggatgtcgc tcagggtccc gtcccacccc ccttcggtgc
     1501 cggtggcgga caggtggcat tcggcgaacg aatcgcccga ggagaagacg agcgaggcct
     1561 tgccgtcgta gctgctcatg gccgcgacgt tacggggcca agatggggtc atgcgacggc
     1621 tgacggcccc ggtcctcgag ggcgcgatgg aggtcgaggc atgaagctcg acgcgatggc
     1681 gatggggctg ccgctgcggg aggcccagcg cctcgccgcc gacatcgagg aagccggctt
     1741 ctccggcctg tggttcaccg agggcggccg caccgcctac ctcggcgcca ccgcggcggc
     1801 gctggccacc tccgagttgg acctgggcac cggcatcgcc gtggccttcc cccgcagccc
     1861 gatgatcacc gcgtcggtgg cctgggagct ggccgaagcg acgggcgggc gcttcgtcct
     1921 cggcctcggc acccaggtca aggcccacat cgagcgccgc tacggcgtga cctacgagca
     1981 ccccgggccg tggctgcggg agtacgtcct cgccctccgg gcgatcttca gggcctttca
     2041 gggcgaggag cgcctcgatt tccacgggga gcactacgac ctcgacctcc tgccgccgac
     2101 gtggagcccc ggccccatcg agcaccccga cgtccccatc tacctgtcgg gcgtgcgccc
     2161 ctggatgctg ggcgcgaccg gtgaggtggc cgacggcctg cacgtccatc cgctgcactc
     2221 ccggcgctac ctcgacgagg tcgtcgtccc caacgtggcc aagggcctgg ccaccgccga
     2281 ccgccccgtc gccgacgtga cgctggcctg cccggtcctg accatcatgg gcgacaccga
     2341 cgaggagcag gatcggtggc gggaggcggc ccggttccag ctcgccttct acggctcgac
     2401 ccggacctac cgcggcgtgt tcgagaccca cggctgggac ggcacctccg agcgcctcca
     2461 cgagcaccag aaggccggcg acttggccgc catggccgcc gtcatcaccg acgagatgct
     2521 ggatgccttc gccgtgaccg ccacctggga cggcctggcc cccgccctgc tcgaccgcta
     2581 cgtcggggtc gagcacccgg tccgggtcat ctgctacttc gccggcaact cgatccgctc
     2641 ggaccagacc ttcgaccagt gggccgccgt cgccgccgcc gtgcgggccg ccgcctcctg
     2701 agccgttccg gcgggttcag gggacgacgt cgcgcggcca gccgtcggcc agggtgccct
     2761 gccaatgcgg gtcccgcttc tcgacccagg ccatgacgcc ctcggtcgtg tcgggcgagc
     2821 ccatcagcgc gacgtgcacc gcccgctccc aggcgtcgac ctcgtcggcg gacggcgccg
     2881 gtgaccgcca gaggatggcc ttgctggcgg ccaccgaccg gggcgccacg ttgacggcga
     2941 tgtcctgcgc gaccgccagt gcccgctcga gcacggccgg accgtcgg
//
LOCUS       DASTFV010000262         6258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_977871, whole genome shotgun sequence.
ACCESSION   DASTFV010000262 DASTFV010000000
VERSION     DASTFV010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977871"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..903
                     /locus_tag="VFP02_04975"
     CDS             184..903
                     /locus_tag="VFP02_04975"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET9542406.1"
                     /translation="MAEATMPTEGSSDSLSVGAVLQSLADERRTGLLRLHGAEPHIVA
                     LADGRVYLATSASGASIHQIVVGSGAAPEAAWADAGLTGDEVAAALDEDERVDSDRLR
                     TVLREHVVATVAELLAPGTERYEFLADQVHQLGPRFCFPVDQVLADAEQRLTTWRSIN
                     TTLPSTGTRIRRSPTLPRDSVSESLSAVEWQVVSAMPTEGTVAEVIAVSGLSAFTVFD
                     VLHRLVRRGLVQPVGEDAAAP"
     gene            900..2042
                     /locus_tag="VFP02_04980"
     CDS             900..2042
                     /locus_tag="VFP02_04980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET9542407.1"
                     /translation="MRGAGLGTVVLLLLLGACDRDAVRVDGQALATAVPQRAEARTGP
                     GLPRAGAAPASGRGLGSGKPITFAFGGDVNFEGTLRTLLADDPTALFAGVTDALRADV
                     TMVNLETAITERGKAEPKEFNFRAPASALTALRTAGVDVVTLANNHGLDFGQEGLVDT
                     LDAAEDPAIGLPLVGAGHDTAEAYSAFRVTRRGQRISILGASRVTDGDLIDDWTVADD
                     RPGIAVAEDPARLLEAVRAERALADTLIVYLHWGRERVNCPIDDQPEMADALIAAGAD
                     VVVGSHAHVSLAGGLRDGAYVHYGLGNFLFGSAVGPSAESGVLRLTLTGRRLDAAEWV
                     PVHLRAGVPEALTGHAAEQAHAEWEDLRPCAQLEAGTERTGEEPDG"
     gene            2035..3330
                     /locus_tag="VFP02_04985"
     CDS             2035..3330
                     /locus_tag="VFP02_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HET9542408.1"
                     /translation="MAEGLDPRATARRLLGHLDASPTPYHAVDRAAELLGAAGFVERD
                     EWAAWTAGDAARWFVRRGGALVAAVWPSALPPEAALRIVGAHTDSPNLRLKPRPQSSS
                     AGWQRLAVEVYGGALLNSWLDRDLGLAGRVSVRRGDEAEVILVHLDGAIARIPQLAIH
                     LDREVNQAGLVLNPQQHLLPLWAPDGEAGVLAAVAAAAACSVDDLMAWDLMLHDVQPA
                     ALTGPGDELLVGGRLDNLVSSFVAVEALAETSEDPDAVGVVCLFDHEEVGSVSRTGAA
                     GELLPGVIERAHRSRGGDPDGLARALSRSACVSADMAHATHPNYADRHDPGHAVDLNG
                     GPVVKVNASQRYASDADSAVPFLEAAERAGVPVQRFVSRSDLPCGSTIGPVTAARLGV
                     STVDVGVPQLAMHSARETGGALDPDLFRRSLGSFLAPSG"
     gene            3400..5292
                     /locus_tag="VFP02_04990"
     CDS             3400..5292
                     /locus_tag="VFP02_04990"
                     /inference="COORDINATES: protein motif:HMM:NF008559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HET9542409.1"
                     /translation="MSHPLDPLTAEEIGRVGAAVRDHVPDTELLFSSITLAEPSKESL
                     GSHRQGPPARAAKVVVVDGGHGVIEALVTTGDGKVCEWTPVADVRPPLLFSEAFNAME
                     AVRADERWRAAVARRGITDFAAVQIDPWPAGAYEDFTERNTRITRCLAYHREDPADNG
                     YARPLEGVVAVVDMVTAEVLAVEDHGVVALPTDPGRYDAEHVGPLRDDLKPLEIHQPE
                     GVSFTVDGNHLAWQRWDLRVGLDPVEGLVLYDVGYQDGDRRRPVLARAALSEMVVPYG
                     SPEPAHRWKNAFDAGEWGLGRFVNSLKLGCDCLGEITYLDAVMADEQGNPFEVEQAIC
                     VHEEDDGIAWKHQDLNTGNVEVRRSRRFVVSAIFTVGNYEYAFYWRFYLDGSIELEVR
                     LTGIIQPCAFEGEPPTGMVVVAPGVAAAHHQHLFCVRLDLDVDGGSNSVYEVDVVPAG
                     GGDDDPWANGIEVRSTLLASEAQAQRDVDPSRSRVWKVVNPGSLNRLGQPVGYKLLPA
                     STPTLLAGPGSVVRDRARFATHNLWVTPYDPAETRAAGPYPNQHTGGDGLPAWTAADR
                     PLVDTDVVLWHSFGLTHVVRPEDWPVMPVERTGFWLVPVGFFDRNPALDVPPSPTARP
                     DRSGCT"
     gene            complement(5332..5730)
                     /locus_tag="VFP02_04995"
     CDS             complement(5332..5730)
                     /locus_tag="VFP02_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HET9542410.1"
                     /translation="MVHPPFALDPPTTGRMLLRALGRRCPLCGSGQVFTWWLVMKDDC
                     PRCDLHFERIEGHWIGAIAMNTILALLVLLGVIVGGFAASYPDSPPAALLWVCLAVAA
                     LTPLLFQPISRTLWTAIDLAMRPIDADELS"
     gene            5776..>6258
                     /locus_tag="VFP02_05000"
     CDS             5776..>6258
                     /locus_tag="VFP02_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HET9542411.1"
                     /translation="MCDTVVVVGDDGVLFAKNSDRDANEGQGLEWHPAADHEAGTSLR
                     CTGIDVPQVARTHAVLLSRPFWMWGAEMGTNEHGVVIGNEAVFTNEPESDTGLLGMDL
                     LRLGLERAASASEAVSVIVDLLERHGQGGGCGHEDRSFTYHNSFLVADRSQAVVLETA
                     G"
BASE COUNT          858 a         2257 c         2257 g          886 t
ORIGIN      
        1 cacggcgatg agggccagca acttggaccc gacgttggcg tttctctgca tggtgtcgcc
       61 cttgctcgat cagcggcgcc cgagacgcaa cgggcgcatt cccatcggcc ccccggtgcg
      121 cttgttgagc ggagggaagg agcgtcaccg acatcctcag agggtcgttg tagcgttgac
      181 ccgatggccg aggcgacgat gccgaccgag ggatcgtcgg actcgctgag cgtcggtgcc
      241 gtcctccagt ccctggctga cgagcggcgc acgggactgc tccgtctcca cggtgccgag
      301 ccgcacatcg tcgccctggc cgacggccgg gtctatctgg ccaccagcgc gtcgggcgcg
      361 tcgatccacc agatcgtggt cggctcgggg gccgccccgg aggcggcgtg ggccgacgcc
      421 ggcctgaccg gggacgaggt cgcagccgcg ctggacgagg acgaacgggt ggacagcgac
      481 cgcttgcgca cggtcctccg ggaacacgtg gtggcgaccg tggccgagct gttggcgccg
      541 ggcaccgagc gctatgagtt cctggccgac caggtccatc agctggggcc ccggttctgc
      601 ttcccggtcg accaggtgct ggccgacgcc gagcagcgct tgaccacctg gcgctcgatc
      661 aacaccaccc tgccgtccac ggggacgcgg atccgccgct cccccaccct gccccgggac
      721 tcggtgtcgg agtcgctttc cgcggtcgaa tggcaggtcg tctcggccat gcccacggag
      781 ggcacggttg cggaggtcat cgccgtgtcc gggctcagcg ccttcacggt gttcgacgtc
      841 ctgcaccgcc tcgtccgccg cggcctggtc caacctgtgg gtgaggacgc tgccgcccca
      901 tgaggggggc gggcctgggg acggtggtgc tcctcctcct cttgggcgcc tgtgaccggg
      961 acgcagtccg ggtcgacggc caggcgttgg ccaccgccgt cccccagcga gccgaggccc
     1021 ggaccgggcc gggcctgccg cgggccggag cggcgcccgc cagtggccgg ggtctggggt
     1081 ccggcaagcc gatcaccttc gccttcggtg gcgacgtcaa cttcgaaggc acgctgcgca
     1141 cccttctggc cgacgacccc accgccctgt tcgcaggggt gaccgacgcc ctgcgggccg
     1201 atgtcacgat ggtcaacctc gagacggcga tcaccgagcg gggcaaggcg gagcccaagg
     1261 agttcaactt ccgggccccg gcctcggcgc tcaccgccct gcgtacggcg ggcgtggacg
     1321 tggtcaccct ggccaacaac cacgggctgg acttcggtca ggagggtttg gtcgacaccc
     1381 tcgatgcggc cgaggatccc gccatcggcc tgccgctcgt cggggccggc cacgacaccg
     1441 ctgaggcgta ttcggccttt cgcgtgaccc ggcgaggcca gcgcatctcg atcctcgggg
     1501 cctctcgggt gaccgatggg gacctgatcg acgactggac ggtcgccgac gaccgtccgg
     1561 gcatcgccgt ggccgaagac cccgctcgcc tgctcgaggc ggtgcgcgcc gagcgggcgc
     1621 tcgccgacac cctcatcgtc tacctccact gggggcggga gcgggtgaac tgccccatcg
     1681 acgaccagcc cgagatggct gacgcgctga tcgccgccgg tgctgacgta gtggtcggca
     1741 gccacgccca tgtctcgctg gcggggggcc tgcgggacgg ggcctatgtc cactacggcc
     1801 tcggcaactt cctgttcggc agcgccgtcg ggccgtccgc ggaatccggc gtgctccggc
     1861 tcacgttgac gggccgccgg ctggacgccg ccgagtgggt cccggtccac ctgcgggcgg
     1921 gcgtgcccga ggcattgacg ggtcacgcgg ccgagcaggc ccacgccgag tgggaggacc
     1981 ttcgcccgtg cgcccagctc gaggcgggca ctgaacggac cggagaggag cccgatggct
     2041 gagggcctgg acccgagggc caccgcgcgg cgcctgttgg ggcatctcga cgcgtcgccg
     2101 accccgtacc acgccgtgga ccgggccgcc gagctgctcg gagcggccgg cttcgtcgag
     2161 cgggacgagt gggcggcctg gacagccggc gatgcggctc gatggttcgt gcggcgggga
     2221 ggcgccctcg tggccgccgt gtggccctct gccctaccgc cggaagctgc cctgcgcatc
     2281 gtcggggctc acaccgacag cccgaacctc cgcctcaagc cgcggccgca gtcgtcgtcc
     2341 gcgggctggc agcgcctggc ggtcgaggtc tacggcggcg ccctgctcaa cagctggctc
     2401 gaccgggacc tcggtctcgc cggccgggtc tcggtccggc ggggcgatga ggccgaggtg
     2461 atcctggtcc acctcgacgg cgcgatcgcc cgcatccccc agctggcgat ccacctcgac
     2521 cgggaggtca accaggccgg tctggtcctc aacccccagc agcacctgct gccgctgtgg
     2581 gctcccgacg gtgaggccgg cgtgctcgcg gcggtggccg cggccgcagc ctgttcggtg
     2641 gacgacctga tggcgtggga cctcatgctc cacgacgtcc agcccgccgc cctgaccggc
     2701 ccgggcgatg agctcctggt cggtggccgc ctggacaacc tggtcagcag cttcgtcgcc
     2761 gtcgaggccc tggccgagac gtcggaggac cccgacgccg tcggcgtggt ctgcctcttc
     2821 gaccacgaag aggtgggctc ggtctcgcgg accggggccg ccggcgagct cctccccggg
     2881 gtgatcgagc gggcccatcg gtcccggggc ggggatccgg atggcctggc tcgggccctg
     2941 tcccgatcgg cgtgcgtctc cgccgacatg gcgcatgcca cccatcccaa ctacgccgac
     3001 cggcatgacc ccggccacgc cgtggacctc aacggcgggc cggtcgtgaa ggtcaacgcc
     3061 tcgcagcgct acgccagtga tgcggacagc gccgtgccgt tcctcgaggc cgccgagcga
     3121 gccggcgtgc cggtccaacg cttcgtcagc cgcagtgacc tgccctgtgg gtcgacgatc
     3181 ggcccggtga ccgccgctcg cctgggcgtg tccacggtgg acgtcggcgt cccccaattg
     3241 gccatgcact ccgcccgcga gaccgggggg gcgctggatc ccgatctgtt ccggcggtcg
     3301 ctggggtcct tcctggcccc ttcaggctga cggcgaggcc ggcgaggccg gcgaggacag
     3361 cacgcccggg aggcgccctg ggcgtgggaa gctgagggca tgagccatcc cctggatccg
     3421 ctgaccgcgg aggagatcgg ccgggtgggc gctgccgtgc gggaccacgt ccccgacacg
     3481 gaactgctgt tctcgtccat caccctcgcc gagccctcca aggagtcgct ggggtcccac
     3541 cggcagggac cgccggcccg agccgccaag gtggtcgtgg tcgacggtgg ccatggggtg
     3601 atcgaggcct tggtgaccac cggggacggc aaggtgtgcg agtggacccc ggtcgccgac
     3661 gtgcgaccgc ccctcctgtt cagcgaggcc ttcaacgcca tggaggccgt gcgagccgat
     3721 gagcgctggc gggcggcggt ggcccggcgc ggcatcaccg acttcgccgc cgtgcagatc
     3781 gacccgtggc cggcgggggc ctacgaggac ttcaccgagc gcaacacccg gatcacccgc
     3841 tgcctggcgt accaccggga ggatcctgcg gacaacggct acgcccgccc gctcgagggc
     3901 gtcgtggccg tggtggacat ggtcaccgcc gaggtgctgg ccgtcgagga ccacggcgtg
     3961 gtcgccctgc ccaccgatcc gggccgctac gacgccgaac acgtcggtcc cctgcgggac
     4021 gacctcaagc cgctggagat ccaccagccg gagggggtca gcttcaccgt cgacggcaac
     4081 cacctggcct ggcagcgctg ggacctccgg gtcggcctgg accccgtgga gggcctcgtc
     4141 ctgtacgacg tgggctacca ggacggcgac cgacggcgcc cggtgctggc ccgggcggcc
     4201 ctctccgaga tggtggtgcc gtacgggtca ccggagccgg cgcaccgctg gaagaacgcc
     4261 ttcgacgccg gcgagtgggg cctcgggcgg ttcgtgaact ccctgaagct gggctgtgac
     4321 tgcctcggcg agatcaccta cctggacgcg gtgatggccg atgagcaggg caacccgttc
     4381 gaggtggagc aggccatctg cgtccacgaa gaggacgacg ggatcgcctg gaagcaccag
     4441 gacctgaaca ccggcaacgt cgaggtccgc cgctcccgcc ggttcgtggt ctccgccatc
     4501 ttcaccgtcg gcaactacga gtacgccttc tactggcggt tctacctcga cggctcgatc
     4561 gagctggagg tccgcctgac cggcatcatc cagccctgcg ccttcgaggg cgagccaccg
     4621 accggcatgg tcgtggtcgc ccccggtgtc gccgccgccc accaccagca cctgttctgc
     4681 gtccgcctcg acctcgatgt cgacggtggg tcgaactcgg tctacgaggt ggacgtggtg
     4741 ccggcgggcg gtggtgacga cgacccgtgg gccaacggca tcgaggtccg gtccacgctg
     4801 ttggcctccg aggcccaggc ccaacgggac gtggacccct cgcgcagccg ggtctggaag
     4861 gtggtcaacc ccggctcgct caaccgcctc ggccaaccgg tcggttacaa gctgctcccg
     4921 gcgtcgaccc cgacgttgct cgccggcccg ggctcggtcg tacgcgaccg ggcccggttc
     4981 gccacccaca acctctgggt cacgccctac gacccggccg agacacgagc cgcggggccc
     5041 taccccaacc agcacacggg cggcgacggc ctcccggcgt ggaccgcggc cgaccgcccc
     5101 ctggtggaca ccgacgtcgt cctgtggcac agcttcggcc tgacccacgt cgtccggccc
     5161 gaggactggc cggtcatgcc cgtcgaacgg acgggcttct ggctcgtgcc ggtgggcttc
     5221 ttcgaccgca atcccgcgct cgacgtcccc ccttccccca ctgcccgacc tgatcgttct
     5281 ggttgcacct agccggcggc agaccgcggg gagacgcaac cagaacggtg atcagctgag
     5341 ctcgtcggcg tcgatgggac gcatggccag gtcgatggcc gtccagaggg tgcgggagat
     5401 gggctggaag agcagcgggg tcagcgcggc gaccgccagg cagacccaca ggagggcggc
     5461 gggcggcgag tccgggtagc tggcggcgaa gccgcccacg atcacgccga gcaggaccag
     5521 cagggccagg atcgtgttca tggcgatggc gccgatccag tggccctcga tgcgctcgaa
     5581 gtgcaggtcg caacggggac agtcgtcctt catgaccagc caccaggtga acacctggcc
     5641 cgacccacag agcgggcacc tccgtccgag ggcccgcagg agcatgcgtc ccgtcgtcgg
     5701 agggtccagc gcgaacggcg ggtgcaccac ggctcggaac ctacccggcg gctggtggcc
     5761 actaccttgc cggacatgtg cgacaccgtg gtcgtggtcg gcgacgacgg tgtgctgttc
     5821 gccaagaaca gcgaccgcga cgccaacgag ggccagggcc tggaatggca cccggcggcc
     5881 gaccacgagg ccgggacctc gctgcgttgc acggggatcg acgtgcccca ggtggcccgg
     5941 acgcacgcgg tcctgctcag ccgacccttc tggatgtggg gggcggagat ggggaccaac
     6001 gagcacggcg tcgtcatcgg caacgaggcc gtcttcacca acgagccgga gtccgacacc
     6061 ggcctgctcg gcatggacct gctccgcctc ggcctcgaac gggcggccag cgcctccgag
     6121 gcggtgtcgg tcatcgtcga cctgctcgag cgccacggcc agggcggcgg ttgcgggcac
     6181 gaagaccgga gcttcacgta ccacaacagc ttcctggtgg ccgatcgcag ccaggccgtg
     6241 gtcctcgaga ccgcgggg
//
LOCUS       DASTFV010000263         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_978823, whole genome shotgun sequence.
ACCESSION   DASTFV010000263 DASTFV010000000
VERSION     DASTFV010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978823"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFP02_05005"
     CDS             <1..353
                     /locus_tag="VFP02_05005"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9542412.1"
                     /translation="HGGQVVKLIGDEVMFVADDPANGLRIARALAAGLPGAEHLPSVR
                     VGLAAGEVLNRDGDYYGSVVNLAARLVTLAEPGEVLLSDAAAAIATDDEVEPLDPVTV
                     KGFPDPVTAFRLLS"
     gene            complement(407..1399)
                     /locus_tag="VFP02_05010"
     CDS             complement(407..1399)
                     /locus_tag="VFP02_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HET9542413.1"
                     /translation="MRLDIRYETRFRYDTPVRESHNELRACPVDDARQRVLDFRLRVD
                     PATPVQSFRDYWGTRVEVFGVIPRHDRLDVIAEISVETHAPAEDPAPATPIADLAGHE
                     AFHEYTVPSGHTGGGDLTELGRTVMNGAESAEETGRTIADWVGRSLTYSPGATKVDTT
                     LDEVVQRRVGVCQDYAHLTVALCRAVDLPARYVSGYLFARRDDAGSGADANGATKEPG
                     EADAVRVQTHAWVEVAVAPGRWQPLDPTNGREVGERHVKIGHGRDYFDVTPFHGSFSG
                     SADPTLVSSVDIRRRSGEDDRPNILSAADRGLLRRPVAQTPQRLLAQQQEQQQQ"
     gene            complement(1396..>2187)
                     /locus_tag="VFP02_05015"
     CDS             complement(1396..>2187)
                     /locus_tag="VFP02_05015"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HET9542414.1"
                     /translation="GKSVRRARENARSVRELLSSELFEAFNRSYLELRASQRRGLRAG
                     LDEHPADLYAAVRLRCQSILGVATETMPRDDPWRFLMLGMLLERAEIMSRMLSVHLIA
                     DHEDPQPASLASWAELLDASAALEAFVRWQRGTAPRITDVTAYLLLSRTFPRSVLRSL
                     RSAEDQLARLESDESRSVCRRLLGGVWSELEYLDGHELTPATLTELLGRVRSRLPAVG
                     NALSDRFFSPGETAGLYLHELRGGPPALGEPERDASPATTAGRPR"
BASE COUNT          290 a          773 c          835 g          289 t
ORIGIN      
        1 cccacggcgg ccaggtcgtg aagctgatcg gcgacgaggt catgttcgtg gccgacgacc
       61 cggccaacgg gctccgcatc gcgcgggcac tggccgctgg gctccccggg gcagaacatc
      121 tgccgtcggt ccgcgtcggc ctggccgcgg gcgaggtcct caaccgggac ggcgactact
      181 acggctcggt ggtcaacctg gccgcccgcc tcgtcaccct ggccgagccc ggcgaggtgc
      241 tcctgtccga cgccgcggcc gcaatcgcca ccgacgacga ggtcgagcca ctggaccccg
      301 tgaccgtcaa gggcttcccc gacccggtca ccgccttccg cctgctcagc tgacgagctc
      361 accgacaacg aggaccgcgg caccgagcct gtcggccacc gacggcttac tgctgctgtt
      421 gttcctgctg ctgggccagg aggcgctgcg gggtctgggc gacggggcgg cggagaaggc
      481 cgcggtcggc cgcggagagg atgttggggc ggtcgtcctc gcccgaccgg cgacggatgt
      541 ccaccgagga gaccagggtg gggtcggcgc tgcccgagaa gctgccgtgg aacggggtga
      601 cgtcgaagta gtcccgaccg tggccgatct tgacgtggcg ctcgccgacc tcacggccgt
      661 tggtcgggtc gaggggctgc cagcgaccgg gggccaccgc cacctcgacc caggcgtggg
      721 tctggacccg caccgcgtcg gcctcgcccg gctccttggt cgcgccgttg gcgtcggcgc
      781 cggacccggc gtcgtcgcgg cgagcgaaca ggtagccgga cacgtagcgg gcgggcaggt
      841 cgacggcccg acagagggcc acggtcaggt gggcgtagtc ctggcagacc cccacccggc
      901 gctggacgac ctcgtcgagg gtggtgtcca ccttggtggc cccggggctg taggtcagcg
      961 agcgccccac ccagtcggcg atcgtccggc cggtctcctc cgccgactcg gccccgttca
     1021 tcaccgtgcg cccgagctcg gtcaggtcgc ccccgcccgt gtgcccgctg ggcaccgtgt
     1081 actcgtggaa ggcctcgtgg ccggccaggt ccgcgatcgg cgtcgcgggc gcggggtcct
     1141 cggccggggc atgggtctcc acgctgatct cggcgatcac gtcgaggcgg tcgtggcggg
     1201 ggatgacccc gaagacctcg acccgggtgc cccagtagtc gcggaaggac tgcaccgggg
     1261 tggccgggtc gacccggagg cggaagtcca ggacccgctg gcgggcatcg tcgacggggc
     1321 aggcccgcag ctcgttgtgg gactcccgca ccggcgtgtc gtagcggaac cgggtctcgt
     1381 agcggatgtc gagcctcatc ggggccggcc cgccgtcgtc gccggtgagg catcccgttc
     1441 cggctcgccg agggccggcg gaccgccccg cagctcgtgc aggtagagcc ccgccgtctc
     1501 gcccgggctg aagaagcggt cgctcagggc gttgccgacc gccgggaggc ggctgcggac
     1561 ccggcccagc agttcggtca gggtcgccgg cgtcagctcg tggccgtcga ggtactcgag
     1621 ctcggaccac acgcccccga ggagccggcg acacaccgat cgactctcgt cggactcgag
     1681 gcgggccagc tggtcctccg cggaccgcaa cgagcgcagc acgctgcggg ggaaggtgcg
     1741 ggacaacagc aggtaggcgg ttacgtcggt gatgcggggg gcggtccccc gctgccagcg
     1801 gacgaaggcc tcgagggcgg cgctggcgtc gaggagctcg gcccacgagg ccagcgaggc
     1861 cggctgcggg tcctcgtggt cggcaatcag gtgcacggac agcatccggc tcatgatctc
     1921 ggcccgctcc agcagcatcc cgagcatgag gaagcgccag gggtcgtcgc gaggcatggt
     1981 ctcggtggcc acaccgagga tggactggca gcgcagccgg accgcggcgt agaggtcggc
     2041 gggatgctcg tcgaggccgg cccgcagccc ccggcgctgc gaggcccgca gctccaggta
     2101 cgagcggttg aaggcctcga agagctccga ggacagcagc tcgcgcacgc tgcgggcgtt
     2161 ctcccgcgcc cggcggacgg acttccc
//
LOCUS       DASTFV010000264         3217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_983719, whole genome shotgun sequence.
ACCESSION   DASTFV010000264 DASTFV010000000
VERSION     DASTFV010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983719"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VFP02_05020"
     CDS             complement(<1..958)
                     /locus_tag="VFP02_05020"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HET9542415.1"
                     /translation="MAATMTHPTERDDAVALRVERLNTASVRRVIEPEERFAWSELTD
                     GQLIADALLSIDPDEYGLTPEQRATLSREEVASLLESGVRFETVLISAFALALAESRH
                     IVDTRSTYALHEMGEETRHSRAFLRLVEHIGPTAPRLLLHGLPMFVRKQIQRGLMRHD
                     VLLYVFVLAGEEIPDLMQKLASEHPETDRLLAEVNRYHRQEEARHLAFARMRLPELQQ
                     EASRWERWRMRHTVPFGIHQLFDSMLDPGIYATVGLPPLRTWAKANRSERRLALRYEA
                     CRPILDAVVAAGFIEADQVPGPWRRLCHVDKAGRPLPDSPALP"
     gene            1043..1636
                     /locus_tag="VFP02_05025"
     CDS             1043..1636
                     /locus_tag="VFP02_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9542416.1"
                     /translation="MDGARVETKGERTRRRLLELAVGRFAEDGYRATSVSAIAREAGL
                     TQAAVYAYFANKEALFEAAVDLDAAELIAGARAQLDEELPLVELIPGLLLHLRLGVEH
                     HRLARRVLAGQEPEVIHRLADLPALQAITYELAGLLQAGQARAEVATDVDAATLAVGV
                     ETVVLALLMSAVQVPGEADSRIPGIVAVFQALFRPPA"
     gene            complement(1710..2681)
                     /locus_tag="VFP02_05030"
     CDS             complement(1710..2681)
                     /locus_tag="VFP02_05030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET9542417.1"
                     /translation="MSTTRYGMTVPFAGLPLGEQRDWFRELVDLGYTDVWSSEADGAD
                     AFTPLTLASVWAPELRLGTAIVPAYTRGPGCLAQTVGSVAQAAPGRFVLGVGSSSNVI
                     VERWNDIPFEEPYKRTRDVVRFLRDALSGEKVRKEYETFSVSGFKLGVRPTEPVPILV
                     AALREGMLRLAGREGDGAIVNWLSADDVSTVAPIVKAFGEDKEVVARIFVAVSEDADA
                     MRAAGRFAIAAYLNVPVYAAFHEWLGRSEALTPMWDAWKAGDRAAALEAIPDEVVDQL
                     IVHGSPEACREHLERYRANGVDTTAVALMPFPGALSQQEAYRALAPR"
     gene            complement(2678..>3217)
                     /locus_tag="VFP02_05035"
     CDS             complement(2678..>3217)
                     /locus_tag="VFP02_05035"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET9542418.1"
                     /translation="AVGRTDDIEILTVNAALGSRPFEPEGEWATMPDVPGPDECGVRT
                     HRFEHESVASRMEQRLAVGRPLDELDGTPSNGNCALWARMPEVLDMSGAALAILGDFV
                     PFGIGQALGLAAGGNSLDNTFRVARLEPTDWVLLDIRVLAVHGGFGHGAVLLWSESGQ
                     LLAIASQSAIVRFWKDQSA"
BASE COUNT          477 a         1115 c         1172 g          453 t
ORIGIN      
        1 cgggcagcgc cgggctgtcc gggagggggc ggccggcctt gtcgacatgg cagagccggc
       61 gccacgggcc cggcacctgg tccgcctcga tgaacccggc cgcgacgacg gcgtccagga
      121 tcgggcggca ggcttcatag cgcagggcga gtcgacgctc ggagcggttg gccttcgccc
      181 aggtccgcag cgggggcagg cccaccgtcg cgtagatgcc cgggtcgagc atcgagtcga
      241 acaactggtg gatgccgaag gggacggtgt ggcgcatccg ccagcgctcc caccggctgg
      301 cctcctgctg cagctcgggc aggcgcatgc gggcgaaggc caggtgtcgg gcctcctcct
      361 ggcggtggta gcggttgacc tcggccagca ggcggtcggt ctcggggtgc tccgaggcca
      421 gtttctgcat caggtcgggg atctcctcgc cggccaggac gaacacgtag agcagcacgt
      481 catggcgcat cagaccgcgc tggatctgct tgcgcacgaa catcgggagg ccgtgcagga
      541 ggaggcgggg ggccgtcggc ccgatgtgct cgaccaggcg caggaaggcc cgggagtgac
      601 gggtctcctc ccccatctcg tgcagggcgt aggtgctgcg ggtgtcgacg atgtggcggc
      661 tctcggccag agccagggcg aaggcggaga tgagcacggt ctcgaaacgg acgccggact
      721 ccaggagcga cgccacctcc tctcgggaga gggtggcccg ctgctcgggc gtgagcccgt
      781 actcgtcggg gtcgatggag agcagggcgt cggcaatgag ctgaccgtcg gtgagctcgc
      841 tccaggcgaa gcgctcttcg ggctcgatga cccgtcggac cgaggccgtg ttgaggcgct
      901 cgacccggag ggcgacggca tcgtcccgtt cggtcgggtg ggtcatggtt gcggccatgg
      961 atcgaccatt ccacaccgtt tcttataagt caatagttct tatagaatta ttgctgtgac
     1021 ctctgccata gcattcggcg ccatggacgg ggcgagggtg gagaccaagg gcgagcgcac
     1081 ccgccggcgc ctgctcgagc tcgccgtcgg ccgcttcgcc gaggacggct accgggccac
     1141 gtcggtgtcg gccatcgctc gagaagccgg cctcacccag gccgcggtct acgcctactt
     1201 cgccaacaag gaggcgttgt tcgaggccgc cgtcgacctc gacgcggccg agctcatcgc
     1261 cggggcccgg gcccaactgg acgaggagct ccccctggtc gagctgatcc ccgggctcct
     1321 cctccacctg cgcctcgggg tcgagcacca tcgcctggcc cgacgggtgc tcgcgggcca
     1381 ggagcccgag gtcatccatc gcctcgccga cctccccgcc ctccaggcca tcacctacga
     1441 gctggcgggc ctgctccagg ccggtcaggc gcgggccgag gtggccaccg acgtcgacgc
     1501 cgccacgctg gccgtcggcg tggagaccgt cgtcctggcg ctgctgatgt ccgccgtcca
     1561 ggtcccgggc gaagccgaca gccgcatccc gggcatcgtc gccgtcttcc aggccctctt
     1621 ccgcccgccc gcctaggcca ggtcggcgcc gatcccgggc ggagagccct gatcaacccc
     1681 ggggctcgtc agggccgtgc cgtcggccat caacgggggg ccagggcccg ataggcctcc
     1741 tgctgtgaga gcgctccggg gaacggcatc agcgccacgg ccgtggtgtc gacgccgttg
     1801 gcccggtagc gctcgaggtg ctcccgacag gcctccggcg agccgtggac gatcagctgg
     1861 tcgaccacct cgtcgggaat ggcctccagg gcggcggccc ggtcaccggc cttccaggcg
     1921 tcccacatcg gcgtcaaggc ctcactccga cccagccact cgtggaacgc ggcgtagacc
     1981 ggcacgttca ggtaggcggc gatggcaaag cgcccggcgg cccgcatggc gtcggcgtcc
     2041 tcggacacgg cgacgaagat gcgggccacg acctccttgt cctcgccgaa ggccttcacg
     2101 atgggcgcga cggtcgagac gtcgtcggcc gaaagccagt tgacgatggc gccgtcacct
     2161 tcccgaccgg cgagccgaag catgccctcc cggagggcgg cgacgaggat gggcacgggc
     2221 tcggtggggc gcacccccag cttgaagccg ctcaccgaga aggtctcgta ctccttgcgc
     2281 accttctcgc cgctgagggc gtcccgcagg aagcggacca cgtcccgggt ccgcttgtag
     2341 ggctcctcga aggggatgtc gttccaacgt tcgacgatga cgttggacga gctgcccacg
     2401 ccgagcacga agcgacccgg ggcggcctgc gccaccgagc ccaccgtctg ggccaggcag
     2461 cccgggcctc gggtgtaggc ggggacgatg gccgtcccca gccggagctc cggagcccag
     2521 accgacgcca gggtcagggg ggtgaaggca tcggccccgt cggcctcgct ggaccagacg
     2581 tcggtgtagc cgaggtcgac cagctcccgg aaccagtccc gctgctcgcc cagcggcaac
     2641 ccggcgaagg ggacggtcat gccatagcga gtcgtactca tgccgactga tccttccaga
     2701 agcggacgat ggcggattgg ctggcgatgg ccaggagctg gcccgattcg gaccagagca
     2761 gcacggcccc gtggccgaac ccgccgtgga cggccagcac ccggatgtcg agcagcaccc
     2821 agtcggtggg ctccaggcga gccacccgga acgtgttgtc caggctgttg cccccggcgg
     2881 ccaggcccaa cgcctggccg atgccgaagg ggacgaagtc gccgaggatg gccaaggcgg
     2941 ccccggacat gtcgaggacc tcgggcatgc gggcccagag ggcgcagttg ccgttgctgg
     3001 gcgtgccgtc cagctcgtcc aaggggcggc ccacggccag acgctgctcc atgcgggagg
     3061 ccaccgactc gtgctcgaag cggtgggtgc ggacgccgca ctcgtcgggg ccggggacgt
     3121 cgggcatcgt cgcccactcc ccctccggct cgaagggtcg agagccgagc gcggcgttga
     3181 cggtgaggat ctcgatgtca tcggtccgac cgacggc
//
LOCUS       DASTFV010000265         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_998781, whole genome shotgun sequence.
ACCESSION   DASTFV010000265 DASTFV010000000
VERSION     DASTFV010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998781"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFP02_05040"
     CDS             <1..197
                     /locus_tag="VFP02_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET9542419.1"
                     /translation="IVGVGGVTDAATALDLLAAGADAIQVGTATFAHPGAPSRVLHQL
                     ETWCRRTRTPHLRELIGTMH"
     gene            194..886
                     /gene="pyrF"
                     /locus_tag="VFP02_05045"
     CDS             194..886
                     /gene="pyrF"
                     /locus_tag="VFP02_05045"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET9542420.1"
                     /translation="MSEAPEELRQKLCLALDVDDLVAANRLARDLKPYFGVVKIGLEL
                     FTAVGPDAIGTMIDQGYKVFVDLKLHDIPNTVERSAQVLGSLGASYLTMHAHGGAPML
                     RAGVTGLHEGAANAGLPDPVAVAVTVLTSDSDAPEHIIPKRVRVAAEGGCQAIVCAVG
                     DLADVRTLTPRLIKVVPGIRPEGAPADDQVRVGTPQEALDEGADLLVIGRPITRADDP
                     VQAATDLVASVG"
     gene            890..1219
                     /gene="mihF"
                     /locus_tag="VFP02_05050"
     CDS             890..1219
                     /gene="mihF"
                     /locus_tag="VFP02_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HET9542421.1"
                     /translation="MPTPPPLSDEQRRAALEKAAEARRVRAQLKADLQAGAISLDDVF
                     ARAAEEKIVADTKILVVLESLPNIGKVRSRRTMAAIGISERQRVRGVGQHQREALKER
                     FAEEEGT"
     gene            1228..1758
                     /locus_tag="VFP02_05055"
     CDS             1228..1758
                     /locus_tag="VFP02_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542422.1"
                     /translation="MAGPGGAGKGTIVERLMARDPQLWLSRSWTTRDRRPGEPRDAYV
                     FTDRDAFMANVKDQGFLEWVEFLDYLQGTPVPDPPEGKDVVLEIDVGGAEQIQAGYPD
                     ELFIFVDAPSRAVQEERLRGRGDPEDKIVQRLGKADQEVDWARELGMHFVINDDLDRA
                     VTEIQGLIAEARASAP"
     gene            1755..2309
                     /locus_tag="VFP02_05060"
     CDS             1755..2309
                     /locus_tag="VFP02_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9542423.1"
                     /translation="MTRWDGGVVTTTDRLTLRTFREDDLPLYAALNADPVVVEFLGGP
                     LSRDGSDRIAAYANDLWAGEGIGLLAVERTGDGTFLGMCGIHVLDSYPDDLEAGWRLA
                     RQHWGRGYATEAAAAWLRIAFEDHGAPRVISVTDPPNRRSIAVMERLGMTVDHEADIE
                     EDGEAFHAVVHAITRGRWFERAAR"
BASE COUNT          374 a          924 c          822 g          334 t
ORIGIN      
        1 ccatcgtggg ggtgggcggg gtgaccgacg ccgccacggc gctcgacctg ctggccgccg
       61 gcgccgacgc gatccaggtc gggacggcca ccttcgccca tccgggtgcc ccatcccggg
      121 tgctccatca actagaaaca tggtgccgtc gcacccgaac cccacacctg cgcgagctga
      181 tcggaacgat gcattgagcg aagccccgga ggaactgcgc cagaagctct gtctggccct
      241 cgacgtggac gacctggtcg ccgccaaccg cctggcccgt gacctcaagc cctacttcgg
      301 ggtggtcaag atcggcctcg agctgttcac ggccgtcggc cccgatgcca tcggcaccat
      361 gatcgaccag ggctacaagg tcttcgtcga cctcaagctg cacgacatcc ccaacacggt
      421 cgagcggtcg gcccaggtcc tcggctcact gggtgccagc tacctgacca tgcacgccca
      481 cggtggcgcc ccgatgttgc gggccggggt caccggtctc cacgaggggg cggccaacgc
      541 cggcctgccc gaccccgtgg ccgtggccgt caccgtcctc accagcgact ccgacgcccc
      601 cgagcacatc atccccaagc gggtccgggt ggcggccgag gggggctgcc aggccatcgt
      661 ctgcgccgtc ggcgacctgg ccgacgtccg caccctcacc ccccgtctca tcaaggtcgt
      721 tcccggcatc cgccccgagg gcgcccccgc cgacgaccag gtccgggtgg gcacgcccca
      781 ggaggctctc gacgaagggg ccgatctcct ggtcatcggt cgtcccatca cgcgggccga
      841 cgaccccgtt caggccgcca ccgacctcgt cgcctccgtc ggctagcgga tgcccacgcc
      901 gccgccgctc agcgacgagc agcgtcgggc cgcactggag aaggcggccg aggcccgtcg
      961 ggtccgggcc cagctcaagg ccgacctgca ggcgggcgcc atcagcctcg acgatgtctt
     1021 cgcccgcgcc gcggaggaaa agatcgtcgc cgacaccaag atcctcgtcg tgctcgagtc
     1081 gctgccgaac atcggcaagg tccggtcccg acggacgatg gccgccatcg gcatcagtga
     1141 gcgccagcgg gtccggggcg tgggccaaca ccaacgcgaa gcgctgaagg agcgcttcgc
     1201 cgaggaggag gggacctgat catcgtcatc gccggcccgg gcggggcggg caagggcacc
     1261 atcgtcgaac ggctcatggc ccgcgacccc cagctctggc tgagccggtc ctggaccacc
     1321 cgcgaccgcc gtcccggcga gccgcgcgac gcgtacgtct tcaccgatcg cgacgcgttc
     1381 atggccaacg tgaaggacca ggggttcctg gagtgggtcg agttcctgga ctacctgcag
     1441 ggcacgcccg tgcccgatcc cccggagggc aaggatgtcg tcctggagat cgacgtcggc
     1501 ggggccgagc agatccaggc cggctacccg gacgagctct tcatcttcgt cgacgccccc
     1561 agccgggccg tccaggagga gcgcctgcgg ggtcggggcg accccgagga caagatcgtc
     1621 cagcgcttgg gcaaggcgga ccaggaggtc gactgggccc gcgagctggg catgcacttc
     1681 gtcatcaacg acgacctcga ccgcgccgtc acggagatcc aaggcctcat cgccgaggct
     1741 cgcgccagcg ccccgtgacc cgttgggacg gcggcgtggt cacgacgacc gatcggctga
     1801 ccctgcgcac cttccgggag gacgacctcc ccctctacgc cgcgctgaac gccgaccccg
     1861 tggtcgtcga gttcctcggc gggccgctga gccgcgacgg ctccgaccgg atcgcggcct
     1921 acgccaacga cctctgggcg ggcgagggca tcggcctgct ggcggtcgaa cgcaccggcg
     1981 acgggacctt tctcgggatg tgcggcatcc acgtcctcga ctcctatccg gacgatctcg
     2041 aggccgggtg gcgactggcc cgccagcact ggggccgcgg gtacgccacc gaggccgccg
     2101 cggcctggct tcgcatcgcc ttcgaggatc acggggctcc ccgggtgatc tcggtgacgg
     2161 acccgcccaa ccgccggagc atcgccgtca tggagcgcct cggcatgacc gtcgaccacg
     2221 aggccgacat cgaggaggac ggcgaggcct ttcacgcggt ggtccacgcc atcacccgcg
     2281 ggcgctggtt cgagcgcgcc gcccggtagc ctgttcaagc ccctttctcc acggagtgct
     2341 gcatgtcttc gcgccatcac acgatgatcg acccccgcat cgaggacctc ctcgagcgcg
     2401 ccggtgactc caagttccgc ctggtgaccc tcggggccaa gcgggcccgg caga
//
LOCUS       DASTFV010000266         3552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1004239, whole genome shotgun sequence.
ACCESSION   DASTFV010000266 DASTFV010000000
VERSION     DASTFV010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004239"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFP02_05065"
     CDS             complement(<1..233)
                     /locus_tag="VFP02_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9542424.1"
                     /translation="MASINEAVDPSAMAAIIAETGAGITFGELEGRSRQLAHLLRERG
                     LGPGSHVAVLLENHVRYFEVCWAAQRSGLYITP"
     gene            292..1215
                     /locus_tag="VFP02_05070"
     CDS             292..1215
                     /locus_tag="VFP02_05070"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9542425.1"
                     /translation="MNLSPQTFEARDGTAISYRRWSARGDPSAIVVVAHGMSEHGGRY
                     ERLATELTAWGWAVYAPDHRGHGATAEATGVGRAGPAGVAGTVDDLFDLIGLARQASG
                     DLPVVLLGHSMGAMLAQAFAVHHGGELAGLALSGSGGYADGLLDLAAMMQGMAEGPMA
                     DEPVPMLPMFNAAFEPGRTDFDWLSRDEAEVDAYLADPLCGAEMPMTFGFTAQLMGLV
                     VDAMSLEGIAALPTDLPVLLLTGEQDPASEMAVNVRALERHLQDAGLEVEAHYYPGAR
                     HEVFNETNRDEVTADLVTWIDGVLAASGPGV"
     gene            1261..2559
                     /locus_tag="VFP02_05075"
     CDS             1261..2559
                     /locus_tag="VFP02_05075"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9542426.1"
                     /translation="MTAVDDLVLISVDDHVIEPPDLFAGHLTGDYLERAPKLVRNEAG
                     NDVWTFGDAVIENPALNAVAGRPLEEYGLEPQSLEEIRPGCYDVDERVKDMDAGGVLA
                     SMNFPSFPTFTARLFATDDVAFSLALVQAYNDWHIDEWCGAHPGRFIPMAVPAIWDPQ
                     LTADEVRRCADKGCHSLTFTENPAALGYPSFHDEYWDPLWRSLCDTDTVLSVHLGSSG
                     KLSIPAVDSPPDVMITLQPMNIQSAAADLLWSRVIKEYPQLRIALSEGGTGWIPYFLD
                     RVDRTFQMHKAWTFQDFGGRLPSEVFREHFLTCFISDPIGVELRHHIGIDNMAWEADY
                     PHSDSMWPTAPEELGTVFADNDVSDADIAKMTHENAMRWYSFDPFAHVPKDEATVGAL
                     RAKAAGHDVSIMSRSTRAQNHEASLAGFRRRAEHALAAPQ"
     gene            2574..>3552
                     /locus_tag="VFP02_05080"
     CDS             2574..>3552
                     /locus_tag="VFP02_05080"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03860.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
                     /protein_id="HET9542427.1"
                     /translation="MSLVGFMQAGNVTVYSGSWRHPATEHGFLDAGYYQKLGRTLEEG
                     CFDLMFFDDRLAMPGIYGGSVAEAVRLGARPVKLDLSIVLGLVAGATSSIGLGATYST
                     TYYTPFHVARTFATLDHLSGGRAAWNVVTSVNDSEAQNYGMKEVLEHDARYDRADEFL
                     EATTGLWDTWEDDALVLDRASGTFADPDKVHELNYEGDWFSVRGPLTVPRCPQGRPVL
                     LQAGSSGRGQEFAAKWAELIFTGDPGIDVARSHYKGQKESIALAGRDPDQVRMLPMAY
                     TVVGESTAHAQEREQLFLNDLVDPNASLTLLSELMNHDFSSMALDDPITD"
BASE COUNT          550 a         1302 c         1174 g          526 t
ORIGIN      
        1 atcggtgtga tgtacaggcc cgaccgctgc gccgcccaac acacctcgaa gtaccggacg
       61 tggttctcga gcagcacggc cacgtggctg cccgggccca ggccccgttc ccggagcagg
      121 tgggccagct gtcggctgcg gccctccagc tcaccgaagg tgatcccggc accggtctcg
      181 gcgatgatcg ccgccatcgc cgacgggtcg accgcctcgt tgatcgatgc catcacacgt
      241 cccccaggat ctcgacatca cgtctgagaa ccagagtatc ctgaggcgga aatgaacttg
      301 tcaccgcaga ccttcgaggc ccgtgacggg acggcgatca gctaccgccg gtggtcggcc
      361 cgcggggatc cgtcggcgat cgtcgtcgtg gcccacggga tgtccgagca cggcggccgc
      421 tacgagcggt tggcgaccga gctgacggcc tggggctggg cggtctacgc ccccgaccac
      481 cggggtcacg gggccaccgc ggaggcgacc ggcgtgggcc gggccgggcc cgcgggcgtg
      541 gccgggaccg tcgacgacct cttcgacctc atcggcctcg cccgccaggc gtcgggcgac
      601 ctcccggtgg tgcttctcgg ccactccatg ggggcgatgc tggcccaggc cttcgccgtg
      661 caccacggcg gcgagctggc cgggttggcc ctttccgggt ccggggggta cgccgacggc
      721 ctcctcgacc tcgccgccat gatgcagggc atggccgagg gcccgatggc ggatgagccc
      781 gtgccgatgc tgcccatgtt caacgccgcc ttcgagcccg gccggaccga cttcgactgg
      841 ctgagccgtg acgaggccga ggtcgacgcc tacctcgccg acccgctgtg cggtgcggag
      901 atgccgatga cgttcggctt caccgcccag ctcatgggac tggtcgtcga cgccatgagc
      961 ctcgagggca tcgccgcgtt gccgaccgac ctgccggtgc tcctgctcac cggggagcag
     1021 gatcccgcct cggagatggc cgtcaacgtg cgggcgctgg aacgccacct ccaggacgcc
     1081 ggcctcgagg tcgaggcgca ctactacccc ggcgcccgcc acgaggtctt caacgagacc
     1141 aaccgggacg aggtcaccgc cgacctggtc acctggatcg acggcgtgct ggcggcctcc
     1201 ggcccgggag tataagctca ctctgctccc acggaagtac cattctccca agggggaacc
     1261 atgaccgcag tcgatgatct cgtcctcatc agtgtcgacg accacgtgat cgagccgcct
     1321 gacctgttcg ccggccacct cacgggcgac tacctggaac gggcgcccaa gctcgtgcgc
     1381 aacgaggccg gcaacgacgt gtggaccttc ggcgacgccg tgatcgagaa cccggcgctg
     1441 aacgccgtcg ccgggcggcc cttggaggag tacggcctcg agccccagag cctcgaggag
     1501 atccggcccg gttgctacga cgtggacgag cgggtgaagg acatggacgc cggcggggtc
     1561 ctggcctcga tgaacttccc ctcctttccg accttcacgg cccggttgtt cgccaccgac
     1621 gacgtcgcct tctccctggc cctcgtgcag gcctacaacg actggcacat cgacgagtgg
     1681 tgcggggccc accccgggcg cttcatcccg atggcggtcc ccgccatctg ggacccccag
     1741 ctcaccgccg acgaggtgcg ccggtgcgcc gacaaggggt gtcactcgct caccttcacc
     1801 gagaaccccg ccgccctggg ctacccgagc ttccacgacg agtactggga cccgctgtgg
     1861 cggtccctct gtgacaccga caccgtgctg tcggtccacc tcgggtcgtc gggcaagctc
     1921 tccatcccgg ccgtcgactc gccccccgac gtgatgatca cgctgcagcc gatgaacatc
     1981 cagtcggcgg ccgccgacct gttgtggtcc cgcgtcatca aggagtaccc gcagctgcgg
     2041 atcgccctgt ccgaaggggg gacggggtgg atcccctact tcctcgatcg ggtggaccgg
     2101 accttccaga tgcacaaggc ctggacgttc caggacttcg gcgggcggct cccgagcgag
     2161 gtgttccggg agcacttcct cacctgcttc atcagcgacc ccatcggtgt cgagctccgc
     2221 caccacatcg gcatcgacaa catggcgtgg gaggcggact acccccacag cgactccatg
     2281 tggccgacgg ccccggagga gttgggcacg gtgttcgccg acaacgacgt gtccgacgcc
     2341 gacatcgcca agatgaccca cgagaacgcc atgcggtggt actccttcga cccgttcgcc
     2401 cacgtcccga aggacgaggc cacggtcggg gcgctgcggg ccaaggctgc cggccacgac
     2461 gtgtcgatca tgtcccgcag cacccgggcc cagaaccacg aggccagcct cgccgggttc
     2521 cggcggcgag ccgaacacgc cctcgccgcg ccgcagtgag caccatgcgc cagatgtccc
     2581 tggtggggtt catgcaggcg ggcaacgtca ccgtctactc gggctcctgg cgccacccgg
     2641 ccaccgagca cggcttcctc gacgccggct actaccagaa gctgggccgg accctcgagg
     2701 agggctgctt cgacctcatg ttcttcgacg accgcctcgc catgcccggc atctacggcg
     2761 gatcggtggc ggaggcggtg cggctcgggg cccgtccggt gaagctcgac ctgtcgatcg
     2821 tgctcggcct ggtggcgggg gcgacgtcgt ccatcgggct cggcgccacc tactcgacga
     2881 cctactacac gccgttccac gtcgcccgca cgttcgccac cctcgaccat ctctccgggg
     2941 gccgggccgc ctggaacgtg gtcacgtcgg tgaacgacag cgaggcccag aactacggga
     3001 tgaaggaggt cctggagcac gacgcccgct acgaccgggc cgacgagttc ctcgaggcca
     3061 cgaccggcct ctgggacacc tgggaggacg acgccctcgt cctcgaccgg gccagcggga
     3121 ccttcgctga cccggacaag gtccacgagc tcaactacga gggcgactgg ttcagcgtcc
     3181 gcggtcccct gaccgtgccc cggtgcccgc aaggccgacc ggtgctcctc caagcggggt
     3241 cgtccggccg gggccaggag ttcgccgcca agtgggccga gctgatcttc acgggggacc
     3301 ccggcatcga cgtcgcccgc tcgcactaca agggccagaa ggagagcatc gccctggcgg
     3361 ggcgggaccc cgaccaggtg aggatgctgc cgatggccta cacggtggtc ggcgagtcca
     3421 cggcgcacgc ccaggagcgc gagcagctct tcctgaacga cctggtcgac cccaacgcct
     3481 cgctcaccct gctctccgag ctgatgaacc acgacttctc gtcgatggcg ctcgacgacc
     3541 cgatcaccga cg
//
LOCUS       DASTFV010000267         3161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1005681, whole genome shotgun sequence.
ACCESSION   DASTFV010000267 DASTFV010000000
VERSION     DASTFV010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005681"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFP02_05085"
     CDS             <1..324
                     /locus_tag="VFP02_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET9542428.1"
                     /translation="MTRGILATCYARPSSPTTTDEVLGLLRDAYVDEPFVTVIDGPPT
                     TKAVLGSNSAMVTARADERTGWVLALCAIDNLVKGASGQAVQCANIVAGLPETLGLPL
                     VGLYP"
     gene            347..1510
                     /gene="argJ"
                     /locus_tag="VFP02_05090"
     CDS             347..1510
                     /gene="argJ"
                     /locus_tag="VFP02_05090"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HET9542429.1"
                     /translation="MSVTAARGFVAAGGHGGLKDDGVSDLAIVATADGQAVPAAGVFT
                     ANKMTAAPVLVSRAHLRQSRGHAAAVFLNSGNANAGTGEPGSQDAEELCAHVAQQLGC
                     AHDQVLICSTGLIGYRLPLEPLVAAIPGLVAQRAPDGDGDAAQAILTTDTVRKEVVID
                     GGGFTVGGMAKGAAMLAPNMATMLAVLTTDAEAEPTVLQKVLQDAVTDTFNCITVDGA
                     TSTNDTVLLLASGVAGPADADVLEAAVTAACASLAEQMVADAEGATKLVRVHVTGAKT
                     DAEARTAARAVASSQLTKCSWYGNDPYWGRVGSDLGACGVAFDPVLLSVAYGGTVVCR
                     HGVEAEHNRAAVTEHMAGRDIELTADLGIGSGRATVLTCDLTHAYVDENMGTS"
     gene            1507..2364
                     /gene="argB"
                     /locus_tag="VFP02_05095"
     CDS             1507..2364
                     /gene="argB"
                     /locus_tag="VFP02_05095"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HET9542430.1"
                     /translation="MSATSGADKATILVEALPYIRRFWGSTVVVKYGGNAMTDPALAM
                     QFAEDIVLMHSVGMRVVVVHGGGPQIGELMAKLGKESEFRDGLRVTDAETLDIARMVL
                     VGKVGRDIVSSINVHAPLAVGLSGEDAGLIVASARDPELGFVGDVDTVDPSILQRLLA
                     EDLIPILSTIGSDAAGQAYNINADTVAGAVGAALGAEKVVYLTDVAGILDGDGELIHQ
                     VDADGINAMVADGTVAGGMIPKVSACLHAVRGGVTSAHVLDGRLPHVLLLELFTDEGV
                     GTMITKDPQ"
     gene            2361..>3161
                     /locus_tag="VFP02_05100"
     CDS             2361..>3161
                     /locus_tag="VFP02_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9542431.1"
                     /translation="MSTLMSTYPPQVVTFVRGRGSELWDTDGKRYLDFLSGLAVTSLG
                     HAHPEVADAVAEQARTLLHVSNLYGTEPGPEVAATLDRLLGGGGKVFFCNSGAEANEA
                     AIKLARKFGGRGRHVVASAYGSFHGRTLATLHATGQPEKHEVFQPLPEGFRHVAWQDL
                     EALEAALDPSVTAVLLEPVQGEGGVNPADTEYFAGVRRLCDERGVLFMVDEIQTGLGR
                     TGEWFGFQHFGVQPDVVTMAKALGNGVPIGACWARGEVAGVFEPGDHAT"
BASE COUNT          458 a         1153 c         1117 g          433 t
ORIGIN      
        1 atgacccgcg ggatcctggc cacctgttac gcgcggccgt cgtcgcccac gacgaccgac
       61 gaggtcctcg gcctcctccg ggacgcgtac gtcgacgagc ccttcgtgac ggtgatcgac
      121 gggccgccga cgaccaaggc cgtgctcggg tccaactcgg cgatggtcac ggcccgggcc
      181 gacgagcgga cgggctgggt cctcgccctc tgcgccatcg acaacctggt caagggcgcc
      241 tccggccaag ccgtccaatg cgccaacatc gtcgccggcc tccccgagac cctcggcctc
      301 cccctggtcg ggctgtaccc gtgaccacgg acgaagaagc ccagccatga gtgtcacggc
      361 agcccggggg ttcgtcgccg ccggtgggca cggcggactg aaggacgacg gcgtgtcgga
      421 cctggccatc gtggccacgg cggacggcca ggcggtgccc gcggcgggcg tcttcacggc
      481 caacaagatg acggcggcgc cggtcctggt gtcccgggcc cacctgaggc agagccgcgg
      541 ccacgccgcc gccgtcttcc tgaactcggg caacgccaac gccggcaccg gtgagcccgg
      601 cagccaggac gccgaggagc tctgcgccca cgtggcccag cagctcggtt gtgcccacga
      661 ccaggtcctc atctgctcca ccggcctgat cggctaccgg ctgccgctcg agcccctggt
      721 cgccgccata cccggcctgg tcgcccagcg ggccccggat ggcgacggtg atgcggccca
      781 ggccatcctc accaccgaca cggtgcgcaa ggaggtcgtg atcgacggcg ggggcttcac
      841 cgtcgggggc atggccaagg gggcggccat gctggccccg aacatggcca cgatgctcgc
      901 ggtcctgacc accgacgccg aagccgagcc caccgtcctc cagaaggtgc tccaggacgc
      961 cgtcaccgac accttcaact gcatcaccgt cgacggggcg acatcgacca acgacaccgt
     1021 cctgctgctg gccagcgggg tcgccggccc ggccgacgcc gacgtcctcg aggcggcggt
     1081 gaccgcggcc tgcgcctccc tggccgagca gatggtcgcc gacgccgaag gggccaccaa
     1141 gctggtccgg gtccacgtca ccggggccaa gaccgacgcc gaggcccgca ccgcggcccg
     1201 ggccgtggcc tccagccagc tgaccaagtg ctcgtggtac ggcaacgacc cctactgggg
     1261 ccgggtgggc agtgacctcg gcgcgtgtgg tgtcgccttc gacccggtcc tgctgtccgt
     1321 ggcctacgga ggcaccgtcg tctgccgcca tggcgtcgag gccgagcaca acagggccgc
     1381 ggtgaccgag cacatggcgg ggcgggacat cgagctgacc gccgacctgg gcatcgggtc
     1441 cggtcgggcc acggtcctga cctgtgacct gacccacgcg tacgtggacg agaacatggg
     1501 gacgtcgtga gcgccacctc gggcgccgac aaggccacga tcctggtcga ggccctgcct
     1561 tacatccggc gcttctgggg cagcacggtc gtggtcaagt acggcggcaa cgccatgacc
     1621 gacccggcgt tggccatgca gttcgccgag gacatcgtgc tgatgcactc ggtggggatg
     1681 cgtgtcgtcg tggtccacgg cggcggtccc cagatcggtg agctgatggc caagctgggg
     1741 aaggagtccg agtttcggga cggcctgcgc gtcaccgacg ccgagaccct ggacatcgcc
     1801 cgcatggtcc tcgtgggcaa ggtcgggcgg gacatcgtgt cgtccatcaa cgtccatgcc
     1861 cccctcgccg tcggcctgtc gggggaggac gccgggctca tcgtggcctc ggcccgcgac
     1921 ccggagctgg gcttcgtcgg cgacgtcgac accgtcgacc cgtcgatcct gcagcgcctt
     1981 ctggccgagg acctgatccc gatcctgtcg accatcggct ccgacgcggc cgggcaggcg
     2041 tacaacatca acgccgacac ggtcgccggc gcagtgggcg ccgccctcgg ggccgagaag
     2101 gtcgtctacc tgaccgacgt ggccgggatc ctcgacggtg acggcgagct gatccaccag
     2161 gtcgacgccg acgggatcaa cgccatggtc gccgacggga ccgtcgccgg gggcatgatc
     2221 ccgaaggtgt cggcctgcct gcacgcggtg cgcggtggtg tgacctcggc ccacgtgctc
     2281 gacggccgcc tcccgcacgt cctgctgctg gagctgttca ccgacgaggg ggtcgggacc
     2341 atgatcacga aggacccgca atgagcacgc tgatgtcgac ctatccgccc caggtcgtga
     2401 ccttcgtgcg gggccggggc agcgagctct gggacaccga cggcaagcgc tacctcgact
     2461 tcctgtccgg cctggccgtg accagcctcg gtcacgccca cccggaggtg gccgacgccg
     2521 tcgccgagca ggcccgcacg ctgctacacg tctcgaacct gtacggcacg gagcccggtc
     2581 ccgaggtggc ggccaccctc gaccgcctcc tgggcggcgg aggcaaggtc ttcttctgca
     2641 actcgggggc ggaggccaac gaggcggcca tcaagctggc ccgaaagttc gggggccgcg
     2701 gccgccacgt cgtggccagc gcctacgggt cgttccacgg ccggaccctg gccaccctcc
     2761 acgccaccgg ccagcccgag aagcacgagg tgttccagcc cctgcccgag ggcttccgcc
     2821 acgtcgcctg gcaggacctg gaggccctgg aggcggcgct cgacccctcg gtcacggcgg
     2881 tcctgctcga gccggtgcag ggcgagggcg gggtcaaccc ggccgacacc gagtacttcg
     2941 ccggggtccg ccggctctgt gacgagcggg gtgtgctctt catggtcgac gagatccaga
     3001 ccggcctggg tcggaccggg gagtggttcg gcttccagca cttcggcgtc cagcccgacg
     3061 tggtcacgat ggccaaggcc ctgggcaacg gggtgccgat cggcgcctgc tgggcccggg
     3121 gcgaggtggc cggggtgttc gagcctggcg accacgccac g
//
LOCUS       DASTFV010000268         3460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1020999, whole genome shotgun sequence.
ACCESSION   DASTFV010000268 DASTFV010000000
VERSION     DASTFV010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020999"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VFP02_05105"
     CDS             complement(<1..399)
                     /locus_tag="VFP02_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542432.1"
                     /translation="MASILAVAALVVLVRRLRQATAKLDEVASRLDSMDDDEPVATPA
                     PPVAPNPVDASSNTLTDQSTGLFSQEYFDVALDARLSAARRHLRPVAVVLIEVVEGLP
                     DSTPRAAAPRTVAESISATIRDADTACRMLD"
     gene            complement(491..2455)
                     /locus_tag="VFP02_05110"
     CDS             complement(491..2455)
                     /locus_tag="VFP02_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HET9542433.1"
                     /translation="MTPSEAHHERDRPWLMRTYSGHSSATASNALYRQNLARGQTGLS
                     IAFDLPTQTGYDPDHPLARGEVGKVGVPVAHLGHMGQLLDGIPVEEMNTSMTINATAA
                     WLLGLYVAHAEDRGVPSEALRGTTQNDIVKEYLSRGTYIFPPLPSRRLIVDMVAYCAT
                     HIPKWNPINVCSYHLQEAGATPVQEIAYAMATAVGVLDAVRDSGQVAEDDFPAVVASI
                     SFFVNAGIRFVEETCKLRAMTALWDRLCLERYRVQDAKARRFRYGVQVNSLGLTEAQP
                     ENNVQRIVLEALGVTLSKDARARSIQLPAWNEALGLPRPWDQQWSLRIQQVLAYETDL
                     LEYEDIFAGSHVVEARTQELLDAATAELDDVLALGGAFEAIDELKTRLVGSHVERMRR
                     IESGDLTVVGVNAYTETAESPLGGAESILRVDPAVQDEQVAELEVWRAERDQDAVKRA
                     LDELARVAATDENLMPATIALAHAGGTTGEWSEALREAFGEYRAPTGVAGATGGATDA
                     LRAIAAELRARPGGPPRLLVAKPGLDGHSNGAEQIAVAGRDAGFEVIYQGIRLTPEQI
                     AAVARDEDVDVIGLSILSGSHLELVPEVLGLLREAGVDAPVVVGGIIPTEDRAVLLEA
                     GVAAVYTPKDFELGALMAELAALTTDVPGT"
     gene            2582..3085
                     /locus_tag="VFP02_05115"
     CDS             2582..3085
                     /locus_tag="VFP02_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542434.1"
                     /translation="MHRSLIRRFLVVALTMAVFLGSGITDASATSLQGANRFSNAPLL
                     PHGEGRYTGNNLTATKQAGEPNHAGDDGGASVWYRFRVGFNQVVRISTRGSNFDTLLA
                     VYRGPDVRHLAVVRQNDDVDIVNGDLTSRVQFPAQAGVTYRIAVDGYDAGGGPERGRI
                     VLNIVTP"
     gene            complement(3075..>3460)
                     /locus_tag="VFP02_05120"
     CDS             complement(3075..>3460)
                     /locus_tag="VFP02_05120"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HET9542435.1"
                     /translation="IAACADPARPHGWINADVRRAYTRLHELGWAHSVEAWAEDELVG
                     GLSGVAVGGLFAGESRFHLRRDATKVALVHLVELLRRGGARLLDVQWTTPHLVSLGAV
                     DISRDEYLTRLGAALKAPLPDAFRA"
BASE COUNT          486 a         1177 c         1282 g          515 t
ORIGIN      
        1 gtcgagcatt cggcaggccg tgtcggcgtc gcggatcgtg gcgctgatgc tttcggcgac
       61 cgtgcggggg gcggccgccc ggggcgtgct gtccggcagg ccctcgacca cctcgatgag
      121 gaccacggcg acggggcgca ggtggcgccg cgccgcggac aggcgggcgt cgagggcgac
      181 gtcgaagtac tcctggctga acaggccggt ggactggtcg gtcagggtgt tggaggatgc
      241 gtcgacgggg ttcggggcca cgggcggcgc gggagtcgcg acgggctcgt cgtcgtccat
      301 ggagtcgagg cgggaggcca cttcgtccag cttggccgtg gcctggcgca ggcgtcggac
      361 gagcacgacc agggccgcga cggcgaggat cgatgccact gcaaccactg caaccatgac
      421 gatcatcgac atccgatcct gatctgtcgc gaaagtagct ggctctccat cggcccggcg
      481 ggcgccgaaa tcaagtcccc ggcacgtcgg tggtcagcgc cgccaactcc gccatcaggg
      541 cccccaactc gaagtccttg ggggtgtaga cggcggccac accggcctcc aggaggacgg
      601 cccggtcttc ggtggggatg atgccaccga cgaccaccgg cgcgtcaacg ccggcttccc
      661 gcagcaggcc caggacctcc gggaccagct ccagatggct gcccgacagg atggacaggc
      721 cgatgacgtc gacgtcctcg tcccgggcca cggccgcgat ctgctccggg gtcaggcgga
      781 tgccctggta gatgacctcg aacccggcat cccgtcccgc cacggcgatc tgctcggccc
      841 cgttggagtg cccgtccagg ccgggcttgg cgaccagcag gcggggtggt ccgcccggtc
      901 gggcccggag ctcggcggcg atggcccgta gggcatcggt ggctcccccg gtcgccccgg
      961 ccacgccggt gggcgcccgg tactcgccga aggcctcccg tagggcctct gaccactcgc
     1021 cggtcgtgcc gccggcgtgg gcgagggcga tggtggccgg catgaggttc tcgtcggtgg
     1081 cggcgacccg ggccagctcg tccagcgccc gcttgaccgc gtcctggtcg cgctcggccc
     1141 gccagacctc gagctcggcg acctgttcgt cctgcaccgc gggatcgacc cggaggatgc
     1201 tctcggcccc acccagcggg gactcggcgg tctcggtgta ggcgttcacg ccgaccaccg
     1261 tgaggtcgcc ggactcgatg cgccgcatgc gttcgacgtg ggagccgacc agacgggtct
     1321 tgagctcgtc gatggcctcg aaggcgcccc cgagggccag gacgtcgtcc agctcggccg
     1381 tcgccgcgtc cagcagctcc tgggtccggg cctcgacgac gtgggagccg gcgaagatgt
     1441 cctcgtactc cagcaggtcg gtctcgtagg ccaggacctg ttggatgcgc aacgaccact
     1501 gctggtccca cggccggggg aggcccaggg cctcgttcca cgccgggagc tggatggacc
     1561 gggccctagc gtccttggag agggtcacgc cgagggcctc gagcacgatg cgttggacgt
     1621 tgttctcggg ctgggcctcg gtcaggccga gcgagttgac ctgcacgccg tagcggaagc
     1681 ggcgggcctt ggcgtcctgg acgcggtagc gctcgaggca cagccggtcc cagagggcgg
     1741 tcatggcccg gagcttgcag gtctcctcca cgaagcggat cccggcgttg acgaagaagc
     1801 tgatggaggc gacgacggcg gggaagtcgt cctcggccac ctggcccgag tcgcgcacgg
     1861 catcgagcac acccaccgcc gtggccatcg cgtaggcgat ctcctgcacc ggcgtggccc
     1921 cggcttcctg gaggtggtac gagcacacgt tgatcgggtt ccacttgggg atgtgggtgg
     1981 cgcagtaggc caccatgtcc acgatcaggc gccggctcgg gagcggcggg aagatgtagg
     2041 taccccggga caggtactcc ttgacgatgt cgttctgggt cgtacctcgg agggcctcgc
     2101 tgggcacgcc ccggtcctcg gcgtgggcca cgtagaggcc gaggagccag gccgcggtgg
     2161 cgttgatggt cattgacgtg ttcatctcct cgacggggat gccgtcgagg agttgcccca
     2221 tgtggccgag gtgggccacg gggacgccga ccttgcccac ctcgccccgg gccagcggat
     2281 ggtcggggtc gtagccggtc tgggtgggga ggtcgaaggc gatcgacagg ccggtctggc
     2341 ctctggccag gttctggcgg tagagggcgt tggacgccgt ggccgagctg tgaccggagt
     2401 aggtgcgcat caaccacggg cgatcgcgct cgtggtgggc ctcagagggg gtcaccgccc
     2461 catcgtcccc cgtgggccgg gcccgtgtca cgcccgccga ggagggggcc cggatcaccg
     2521 gcaccatcgc tcacgctggg gtaatgtgcc gccccagcca agccctatac cggaggtcgt
     2581 tatgcaccgt tcgctcatcc gtcgtttcct ggtcgtcgcg ttgacgatgg ccgtctttct
     2641 cgggtccggt atcaccgatg cctcggcgac ctcgctccag ggcgccaacc ggttctccaa
     2701 cgcgccgctg ctgccccacg gtgaagggcg gtacaccggg aacaacctca cggccaccaa
     2761 gcaggccggc gagccgaacc acgcaggcga cgacggtggg gcctcggtct ggtaccgctt
     2821 ccgggtgggc ttcaaccagg tcgttcgcat ctcgacccgc ggcagcaact tcgacaccct
     2881 gcttgccgtc taccggggcc cggacgtccg gcacctcgcg gtggtccggc agaacgacga
     2941 tgtggacatc gtcaacggcg acctgacgag ccgcgtccag ttcccggcgc aggccggggt
     3001 gacctaccgg atcgcggtcg acgggtacga cgcgggcggc ggcccggaac ggggtcggat
     3061 cgttctcaac atcgtcacgc cctgaacgcg tctgggagcg gcgccttgag cgccgcaccc
     3121 agacgcgtca ggtactcgtc gcgggagatg tcgaccgcgc ccagtgagac gaggtggggg
     3181 gtggtccact ggacgtcgag cagacgggcg cccccgcgcc ggagcagctc gacgaggtgg
     3241 accagggcca ccttcgtggc gtcgcggcgg aggtggaacc tcgactcgcc ggcgaagagc
     3301 ccgcccacgg ccacgccaga gagcccaccg accagctcgt cctcggccca cgcctcgacg
     3361 ctgtgggccc agcccagctc gtggagccgg gtgtaggccc ggcgcacatc ggcgttgatc
     3421 cagccgtggg gccgggccgg gtcggcgcag gctgcgatca
//
LOCUS       DASTFV010000269         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1021976, whole genome shotgun sequence.
ACCESSION   DASTFV010000269 DASTFV010000000
VERSION     DASTFV010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021976"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VFP02_05125"
     CDS             complement(<1..581)
                     /locus_tag="VFP02_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET9542436.1"
                     /translation="MTLRSLPALLRDEPVLADVLGATTTTLTVPEVARAIFVAGLAEV
                     SSRHPIVVATPTSTEAERLAHDLRVFCGEDGVDVFPAWETLPFERVSPAVETMGRRLR
                     TIWHLTDPDQAPRIVVAPIRALVQRLGPHVEDIAPVIIRAGSQVDQEGLVSQLVHLGY
                     RREYQVEHRGEVAVRGSIVDVFPSTADQPVRIDL"
     gene            complement(637..1257)
                     /gene="pth"
                     /locus_tag="VFP02_05130"
     CDS             complement(637..1257)
                     /gene="pth"
                     /locus_tag="VFP02_05130"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET9542437.1"
                     /translation="MRRRGERGERRGTPADLLVVGLANPGKDYEGTRHNVGAAAVHLL
                     AHRHGARLKGQRGVPATIGEVTVAGRRLALAVPTTYMNESGQAVRPLVRRFGVEHELG
                     KLVVVHDELDLPPGRLKVKVGGGLAGNNGLRSIRDHLHTTEFLRVRIGVGKPPSKERG
                     ADHVLARPGKRESLELEIAVQEAADAVEVILAEGVERAMERVNARE"
     gene            complement(1261..1956)
                     /locus_tag="VFP02_05135"
     CDS             complement(1261..1956)
                     /locus_tag="VFP02_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HET9542438.1"
                     /translation="MDVTLTAETGRALGSRSSRRLLRDGKVPAVVYGLDQDTVAVAVE
                     WPSLRAALTTDAGLNALITLEVDGREDLTIVKDLQRDPVRREVVHVDFLRVSRDVEIE
                     VEVPIVLEGHAEKVEREDGTVAHLLFSLSVYAKPGAIPNELTVDVAEMELHDSIRVGD
                     LSLPSGVRTEVDEDEVIVSAQISRETIEVAEEEEFIEALDELAEAAAEAGEGGGEADA
                     GGDDSGGDASDEG"
     gene            complement(2024..>2878)
                     /locus_tag="VFP02_05140"
     CDS             complement(2024..>2878)
                     /locus_tag="VFP02_05140"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET9542439.1"
                     /translation="EIHCRYNESIRGADVFIVQSHTHLDGLTLNDSLMEQLIMVDAAK
                     RASAKRISVVCPYYGYARQDRKAEGREPISAKLVADLFRAAGATRLISVDLHSGQIQG
                     FFDGPVDHLTAAPVLIDYCHRDLPDNLVIVSPDTGRVKVAEKYANKLGADIAIVHKRH
                     VAGQKNAVEAREVIGVVEGRPCVVLDDMIDTAGTIVAAAELLIERGASEVWAMATHGL
                     FSGPAVDRLKNSKIDKVVVTNTLPLPPEKQFDKIEVLSVAPVIAKAIRAVFEDNSVSE
                     IFDGENLS"
BASE COUNT          390 a          986 c         1058 g          444 t
ORIGIN      
        1 aggtcgatgc gcaccggctg gtcggcggtg gaggggaaca cgtcgacgat cgagccccgg
       61 acggcgacct cgccccggtg ctcgacctgg tactcgcggc ggtagccgag gtggaccagt
      121 tggctgacca gaccctcctg gtcgacctgc gagccggcgc ggatgatcac gggggcgatg
      181 tcctcgacgt gagggccgag gcgctggacc agggcccgga tgggggcgac cacgatgcgg
      241 ggggcctggt cggggtcggt caggtgccag atggtccgca ggcggcggcc catcgtctcc
      301 accgccggac tgacccgctc gaagggcagg gtctcccacg ccgggaacac gtcgacgccg
      361 tcctcgccgc agaagacccg caggtcgtgg gccaggcgtt cggcttcggt gctggtcggc
      421 gtggccacca cgatcgggtg gcggctggag acctcggcca agccggcgac gaagatggcc
      481 cgggccacct ccgggacggt cagggtcgtc gtggtcgcgc cgaggacgtc ggccaggacg
      541 ggctcgtccc gcagcagcgc ggggagcgag cggagggtca ccgcccgaat ctagggccgc
      601 cccagcgacg tgcggcgggg cgggacgtgc ttctcgtcac tcccgggcgt tgacccgttc
      661 cattgcccgc tcgaccccct cggccaggat cacctcgacg gcgtcggcgg cctcctgcac
      721 ggcgatctcc agctcgaggc tctcccgctt gccggggcgg gccaggacgt gatcggctcc
      781 ccgctccttc gacgggggct tgccgacgcc gatgcggacc cgcaggaact cggtggtgtg
      841 caggtggtcc cggatcgatc ggaggccgtt gttgccggcc agaccccctc cgaccttgac
      901 cttgaggcgg ccgggcggca ggtcgagctc gtcgtggacg acgaccagct tccccagctc
      961 gtgctcgacg ccgaagcgcc gcaccagggg gcggacggcc tggcccgact cgttcatgta
     1021 cgtggtcggc acggccaggg ccagacggcg accggcgacg gtcacctcgc cgatcgtggc
     1081 ggggaccccc cgctggccct tcaggcgggc gccgtggcgg tgggccagga ggtggacggc
     1141 tgcggccccg acgttgtgcc gggtgccctc atagtccttg cccgggttgg ccagcccgac
     1201 gaccagcagg tcggcgggcg tgcctcggcg ctcgccgcgc tcgccgcggc ggcgcaccga
     1261 ctaaccctcg tcggaggcgt cgccgcccga gtcgtcgcca ccggcatcgg cctcgccgcc
     1321 accttcgccc gcttcggccg cggcctcggc gagctcgtcg agggcctcga tgaactcttc
     1381 ctcctcagcc acctcgatgg tctcgcggct gatctgggca ctgacgatga cctcgtcctc
     1441 gtccacctcg gtgcgcacac cgctgggcag gctcaggtcg ccgacccgga tcgagtcgtg
     1501 gagctccatc tcggcgacgt cgacggtgag ctcgttgggg atggcgcccg gcttggcgta
     1561 gaccgagagg ctgaagagca ggtgggcgac ggtgccgtcc tcccgctcga ccttctcggc
     1621 atggccctcg agcacgatcg ggacctcgac ctcgatctcg acgtcacggg acacccgcag
     1681 gaagtcgacg tggacgacct cacggcggac cgggtcccgc tggaggtcct tgacgatggt
     1741 caggtcttcg cgcccgtcca cctccagggt gatcagggcg ttgaggccgg catcggtggt
     1801 cagcgcggcc cgcagggagg gccactcgac ggccacggcg acggtgtcct ggtccaggcc
     1861 gtacacgacg gccggcacct tgccgtcccg aaggagacgc cgcgacgagc ggcttccgag
     1921 cgcgcggccg gtctcagcgg tgagggtgac gtccatcagg gaactccgtg ttcagggtgg
     1981 agagatgcga cccggaaggc taccggcgga agacgccgag ccgtcagctg aggttctcgc
     2041 cgtcgaagat ctccgacacg gagttgtcct cgaacacggc ccggatggcc ttggcgatga
     2101 ccggggccac cgagagcacc tcgatcttgt cgaactgctt ctccggcggc agcggcagcg
     2161 tgttggtgac cacgaccttg tcgatcttgg agttcttcag ccggtccact gccggccccg
     2221 agaagaggcc gtgggtggcc atggcccaga cctcgctggc cccccgctcg atgagcagtt
     2281 cggcggcggc cacgatggtg cccgccgtgt cgatcatgtc gtcgagcacg acgcaggggc
     2341 gcccctcgac gacgccgatc acttcacggg cctcgaccgc gttcttctgg ccggccacgt
     2401 ggcgcttgtg gacgatggcg atgtcggccc cgagcttgtt ggcgtacttc tcggccacct
     2461 tcacccgccc ggtgtccggc gacacgatga ccaggttgtc gggcaggtcc cggtggcagt
     2521 agtcgatgag gaccggcgcc gcggtcaggt ggtcgaccgg gccgtcgaag aatccctgga
     2581 tctggccgga gtgcaggtcg acgctgatga gccgggtggc cccggcggcc cggaacaggt
     2641 cggccacgag cttggcgctg atgggctcgc ggccctcggc cttgcgatcc tggcgggcgt
     2701 agccgtagta ggggcagacc acgctgatgc gcttggccga cgcccgcttg gcggcgtcga
     2761 ccatgatcag ctgctccatg agcgagtcgt tcagggtcag cccgtcgagg tgggtgtggg
     2821 actggacgat gaagacgtcg gcgccccgga tggactcgtt gtaccggcag tggatctc
//
LOCUS       DASTFV010000270         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1039627, whole genome shotgun sequence.
ACCESSION   DASTFV010000270 DASTFV010000000
VERSION     DASTFV010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039627"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFP02_05145"
     CDS             complement(<1..196)
                     /locus_tag="VFP02_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET9542440.1"
                     /translation="MAEFTAKDVQALRQKTGAGMMDAKKALQENDGDFEAAAKWLREK
                     GLASSAKRSDRANEQGAVAVG"
     gene            complement(199..1308)
                     /gene="rpsB"
                     /locus_tag="VFP02_05150"
     CDS             complement(199..1308)
                     /gene="rpsB"
                     /locus_tag="VFP02_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET9542441.1"
                     /translation="MAPVVTMKQLLDAGVHFGHQTRRWNPKMKRFLHGERQGIYIIDL
                     EQTLDRVRVAYEFVRDMTADGGNLLFVGTKKQASDAVEQYAMSCNMPYVNQRWLGGML
                     TNFETISKRVSKMADLEHQRDSGEFDAMPKKEALMNTRELDKLLRNLNGIRNLDRKPD
                     AVFVLDTKKEHIAVTEANKLGIPLIAVVDTNCDPDVVDYVIPGNDDAIRSGSLMCRIM
                     ADAVKEGRYIRSKKVPGDGGPPAAPALRTPEEEAEIARQQAEARDEAARQAAEREARV
                     AAAKAEADAATDAPAASVGQAPAAEAPAEEAPAAEAPAEEAVAPSADAPADEAVTGEA
                     EAPVEEAAGPEADAPAAEVAVETPEAATTETTEEG"
     gene            complement(1410..2003)
                     /locus_tag="VFP02_05155"
     CDS             complement(1410..2003)
                     /locus_tag="VFP02_05155"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET9542442.1"
                     /translation="MSRHRSFLVLLLLALTAGLAGPAAAEPAIYRPPVDAPVVDGFRL
                     PNGPFGAGNRGLEYDTVPGQVVRAIGDGLVVFAGPVAGIRYVTVLHPDGLRSSYGYLA
                     DLLVAAGERIVIGQAMGTAGERLHLGVRSGGTYLDPAALFTTTGVHLVPLESGPPHVS
                     GSRDASPATRGVDQNAAWAWLTGGGAGGVAGTLARRS"
     gene            complement(2054..>2318)
                     /locus_tag="VFP02_05160"
     CDS             complement(2054..>2318)
                     /locus_tag="VFP02_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9542443.1"
                     /translation="LGDTIADSGDGPVSRFEVEETRQLLASTINRLPEREKLVLTLYY
                     YEGLTLAEIGGVLGVTESRVCQIHTKAVLHLRSRVVAAERSPA"
BASE COUNT          300 a          803 c          842 g          373 t
ORIGIN      
        1 cgccgacggc gacggcgccc tgctcgttgg cccggtcgga gcgcttggcc gaactggcca
       61 ggcccttctc gcgcagccac ttggcagcag cctcgaagtc cccgtcgttc tcctgcaggg
      121 ccttcttggc gtccatcatc ccggcccccg tcttctgacg gagcgcctgg acgtccttgg
      181 cggtgaactc ggccacgatc agccttcctc ggtggtctca gtggttgcgg cctcgggggt
      241 ctcgaccgcg acctcggcgg ccggggcatc cgcctccggg cccgcggcct cctccacggg
      301 ggcctcggcc tcaccggtga ccgcttcgtc ggccggtgcg tcggccgaag gggcgacggc
      361 ctcctcggcc ggggcttcgg cggccggggc ctcctcggcc ggggcttcgg cggccggggc
      421 ctgcccgacc gatgccgcgg gagcgtcggt tgcggcatcg gcctcggcct tggccgcggc
      481 gacacgagcc tcacgctcgg cggcctggcg ggccgcctcg tcgcgggcct ccgcctgctg
      541 gcgggcgatc tcggcctcct cctcgggggt gcgcagggcg ggggcggccg gggggccacc
      601 gtcgccgggc accttcttgg agcggatgta acgcccttcc ttcacggcgt cggccatgat
      661 ccggcacatg aggctgcccg agcggatggc gtcgtcgttg ccggggatca cgtagtccac
      721 gacgtcggga tcacagttgg tgtccaccac ggcgatcagc gggatgccga gcttgttggc
      781 ctcggtcacc gcgatgtgct ccttcttcgt gtccagcacg aagacggcgt cgggcttgcg
      841 atccaggttc cggatgccgt tcaggttgcg caggagcttg tccagctccc gcgtgttcat
      901 cagggcttcc ttcttgggca tggcgtcgaa ctcgccggag tcgcgctggt gctccaggtc
      961 ggccatcttg gagacccgct tggagatggt ctcgaagttg gtgagcatgc cgcccagcca
     1021 gcgctggttg acgtagggca tgttgcagga catcgcgtac tgctcgacgg catcggacgc
     1081 ctgcttcttc gtgccgacga agagcaggtt cccgccgtcg gcggtcatgt cgcggacgaa
     1141 ctcgtaggcg acgcggaccc ggtccagcgt ctgctcgaga tcgatgatgt agatcccctg
     1201 gcgctccccg tggaggaagc gcttcatctt ggggttccag cgccgggtct gatgtccgaa
     1261 gtggacacct gcgtccagca gctgcttcat ggtgacgact ggcgccatct ggttctttcc
     1321 tccccgacgg ttctgacgaa gcccgtgcgc ttgcgcccgg aggcgaccgc gggaatggca
     1381 cgggtgaact ggtctgcggg ccgtggtggt cacgaccgac gggcgagtgt accggcgacg
     1441 cccccggccc caccgcccgt cagccaggcc cacgccgcgt tctgatccac cccgcgtgtc
     1501 gccggcgacg catctcggga cccagaaacg tgaggtggcc ctgactccag cgggaccaga
     1561 tggaccccgg tcgtcgtgaa gagggcggcc gggtccaggt aggtcccccc cgaccgcacg
     1621 ccgaggtgga gccgctcccc cgccgtcccc atggcctggc cgatgacgat ccgttcgccc
     1681 gcggcgacca agagatcggc caggtagccg tagctggagc ggaggccgtc ggggtggagc
     1741 acggtcacgt agcggatgcc ggcgacgggt ccggcgaaga ccaccagccc gtcgccgatg
     1801 gcccggacga cttggccggg aacggtgtcg tactccagcc cccggttccc ggcaccgaag
     1861 ggcccgttcg ggaggcggaa gccgtcgacc accggggcgt ccaccggtgg tcggtagatc
     1921 gcgggctcgg cggcggcggg cccggcgagg cccgcggtca gggcgagcag gagcaggacg
     1981 aggaacgagc ggtgacggga cacacgggga cccccggaga gactctcgac gggggaagcg
     2041 ccggaccagg aggctaggcg ggtgagcgct ccgccgccac gacccgcgat cgcaggtgga
     2101 ggaccgcctt ggtgtggatc tgacacaccc gactctcggt caccccgagg acgccgccga
     2161 tctcggccag cgtcaggcct tcgtagtagt agagggtcag gaccagcttc tcccgctccg
     2221 gcaggcggtt gatggtcgag gccagcagct ggcgggtctc ctcgacctcg aagcggctga
     2281 ccggaccgtc gccgctgtcg gcgatcgtgt cgcccagc
//
LOCUS       DASTFV010000271         3010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1040618, whole genome shotgun sequence.
ACCESSION   DASTFV010000271 DASTFV010000000
VERSION     DASTFV010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040618"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VFP02_05165"
     CDS             complement(<1..565)
                     /locus_tag="VFP02_05165"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HET9542444.1"
                     /translation="MSAGTLRDLHQRRPLVLPNVWDAASARAFAAAGFPALATSSGAV
                     ANVLGHEDHEQAPVDEVLAAIARITRSVDIPVTADFESGYGLAPDEIVDRLLEAGAVG
                     CNLEDTDHAVGAPRPVAVQAERLGAVVDLAAGRLVVNARVDTYVSGDGDDDDAIARAT
                     AYLAAGADCAYPIGRLDDATTARLAEAI"
     gene            684..2069
                     /locus_tag="VFP02_05170"
     CDS             684..2069
                     /locus_tag="VFP02_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET9542445.1"
                     /translation="MATTWASSVDLLVDLTEVDGRRSALERALRDAIRDRRLLAGDRL
                     PSSRALAADLGVARGTVTEAYAQLTAEGWLVARQGAATTVAPGAGGEPPKAVITAPAP
                     RPRHDFKPGSPDLSAFPRRAWAAAQRQVLRTMPDADLGYGHPFGHPTLREALSAYLGR
                     VRGVVTDADHVVGCSGWSQALDLTCRVLATRGVAAVGMEDPCIPHYRATVRAAGLEVV
                     PLPVDDDGARLDDIDGVGAIVLTPAHQYPSGPTLAPERRAAAVAWARATGGLVVEDDY
                     DGELRYDRQPVGALQALDPEHVLYVGTAAKSLAPGLRLAWMAVPSALREDLFQAKHLA
                     DRHTSVLEQLTLAELVTSGTYDRQLRGARVRYRRRRDALLEALSDAVPHLEPSGIAAG
                     LHVVLDLDGRPEAPVVDGLLAREVGVGALGPSYHDEARRTGVVVGYGTPPEHGFPAAL
                     DALVTGLAALA"
     gene            2131..2724
                     /locus_tag="VFP02_05175"
     CDS             2131..2724
                     /locus_tag="VFP02_05175"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET9542446.1"
                     /translation="MRFRTRTSIIADAALLGLRGIVGGYVAAHGAQKMFGVLNGPGLD
                     RAGQHFEEDLDLAPGRERAAAAAGSELVGGTLTAAGLGGPLGPMAIIGTMTVASQTAH
                     RGKGPFVADGGPELTLTNVAAAATIALVGPGRFSLDRVLRVRPSKLLIALAAWAGVGA
                     SVGVIQGQVVRRAEQQSRPAPAASRVEPEYVDLLRSA"
     gene            2820..>3010
                     /locus_tag="VFP02_05180"
     CDS             2820..>3010
                     /locus_tag="VFP02_05180"
                     /inference="COORDINATES: protein motif:HMM:NF036807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9542447.1"
                     /translation="MTIRVVGAGLGRTGTTSLKAALEQLLGGPCHHMFEVAFNPEQVP
                     MWTAAARGEPVDWDEVLDG"
BASE COUNT          380 a         1131 c         1104 g          395 t
ORIGIN      
        1 gaatggcctc ggccaggcgg gccgtggtgg cgtcgtccag ccggccgatg gggtacgcgc
       61 agtcggcccc tgcggcgagg taggccgtgg cccgggcgat ggcgtcgtcg tcgtcgccgt
      121 ccccggacac gtacgtgtcg acccgggcgt tgacgaccag gcgcccggcg gcgaggtcga
      181 cgacggcgcc gaggcgctcg gcctgcacgg ccacgggacg aggggcgccg accgcgtggt
      241 cggtgtcctc gaggttgcag cccacggcgc cggcctcgag gagacggtcg acgatctcgt
      301 ccggggccag gccgtaaccg gattcgaagt ccgccgtgac cgggatgtcg accgaccggg
      361 tgatccgggc gatggcggcc aggacctcgt cgaccggcgc ctgctcgtgg tcctcgtggc
      421 cgaggacgtt ggccacagcc ccgctgctgg tggcgagggc ggggaacccg gcggcggcga
      481 aggcgcgggc cgacgccgcg tcccagacgt tgggcagcac cagcggccgc cgctggtgca
      541 ggtcccggag cgtcccggcg ctcacgcccg caccgccgat gcgaggcggg agggttcgac
      601 ggactcggcg gtcttccaag ggacgatgcg ttccacagaa aggaacctac ggccgaagtg
      661 gtccaacgat aaggtccatt ccgatggcaa cgacgtgggc cagttcggtg gatctcctcg
      721 tcgacctgac cgaggtcgac ggtcgacggt ccgccctcga acgggccctg cgggacgcca
      781 tccgcgatcg acgcctcctc gccggcgacc gcctcccctc gtcccgggcg ctggccgccg
      841 acctgggtgt ggcccggggc accgtgaccg aggcctacgc ccagctcacc gccgaggggt
      901 ggctggtggc ccgtcagggg gcggcgacga ccgtggcgcc gggagcaggg ggcgagccgc
      961 ccaaagcggt catcaccgcc cccgcgccgc gaccccggca cgacttcaag ccgggcagcc
     1021 ccgatctgtc ggcctttccc cggcgggcat gggccgcggc ccagcgccag gtcctgcgca
     1081 ccatgccgga cgccgacctc ggctacgggc accccttcgg ccatcccacc ctgcgcgagg
     1141 ccttgtcggc ctatctcggt cgggtgcggg gcgtggtcac cgacgccgac cacgtcgtgg
     1201 ggtgctcggg ctggagccag gcgctggacc tgacctgccg ggtcctggcc acccggggcg
     1261 tcgccgccgt gggcatggag gacccgtgca tcccccacta ccgggccacc gtccgggccg
     1321 cggggctgga ggtcgtaccc ctgcccgtgg acgacgacgg cgcccgcttg gacgacatcg
     1381 acggcgtcgg tgccatcgtc ctcacgccgg cccaccagta cccgtcaggc ccgacgctcg
     1441 cgcccgagcg gcgggccgcg gccgtggcct gggcccgcgc caccggcggg ctcgtcgtgg
     1501 aggatgacta cgacggcgag ctccggtacg accgtcagcc cgtcggggcg ctgcaggccc
     1561 tggacccgga gcacgtgctc tacgtcggca ccgcggccaa gagcctggcc cccggcctgc
     1621 gcctcgcatg gatggccgtg cccagcgccc tgcgcgagga cctgttccag gccaagcacc
     1681 tcgccgaccg ccacacctcg gtgttggaac agctcaccct cgccgagctg gtgacttcgg
     1741 ggacctacga ccgccagctc cgcggggccc gggtccgcta ccggcgacgc cgggacgccc
     1801 tcctcgaggc cctgtccgac gcggtgcccc acctcgagcc ctccgggatc gccgccgggc
     1861 tccatgtcgt cctcgacctc gacggtcgac cggaagcccc cgtcgtcgac gggctgctgg
     1921 cccgggaggt gggcgtcggt gccctcgggc cctcgtatca cgacgaggct cgacgcaccg
     1981 gcgtcgtcgt cggctacggc acgccccccg agcacggctt cccggccgcg ctggacgccc
     2041 tcgtcaccgg gctcgccgcc ctcgcctgaa aatccacata caggcctccc gccagtcatc
     2101 agcggcggcc gagcggggta ggcgtgacct atgcgcttca ggactcgcac gtccatcatc
     2161 gccgatgccg ccctcctcgg cctgcggggg atcgtcggcg gctacgtcgc cgcccacggc
     2221 gcccagaaga tgttcggcgt gctcaacggc cccggcctcg accgagccgg gcaacacttc
     2281 gaagaggact tggacctggc tccgggtcgg gagagggccg cggcggccgc cgggtcggag
     2341 ctggtgggcg ggacgctgac cgcggcgggt ctgggcggcc ccctcggccc gatggcgatc
     2401 atcgggacga tgaccgtggc cagccagacg gcccaccggg gcaagggccc gttcgtcgct
     2461 gacggcggcc cggagctcac gctgacgaac gtcgcggcgg cggcgaccat cgccctggtc
     2521 ggcccgggac ggttcagcct cgatcgtgtg ctgcgggtcc gcccgtcgaa gctgctgatc
     2581 gcgctggcgg cgtgggccgg cgtcggcgcc agcgtcggcg tcatccaggg tcaagtcgtc
     2641 cgccgcgccg agcagcagtc gcgtccggcg cccgccgcca gccgggtcga gcccgagtac
     2701 gtcgacctcc tccgctccgc ctgatcgaac cccgacccgc cccacccgct tctggcctct
     2761 gatctgctcc ctctgacgag cagatcagag gccagaacgg tgcgatacgg tcgccggcca
     2821 tgaccatccg ggtggtgggg gcgggtctgg ggcggacggg cacgacctcg ctgaaggcgg
     2881 cgctcgagca gctactgggc gggccgtgcc atcacatgtt cgaggtggcc ttcaaccccg
     2941 agcaggtgcc gatgtggacc gccgccgccc ggggcgagcc cgtcgactgg gacgaggtgc
     3001 tcgatggcta
//
LOCUS       DASTFV010000272         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1044734, whole genome shotgun sequence.
ACCESSION   DASTFV010000272 DASTFV010000000
VERSION     DASTFV010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044734"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VFP02_05185"
     CDS             <1..894
                     /locus_tag="VFP02_05185"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET9542448.1"
                     /translation="DSMGTSVVVSSTGIRSGGSETVCDLVAYALAEEAGETQTGFGFS
                     VGRSPGELDVDEAAKDAVYRATRLLGATKPPSQRLTVVLDPFVTAQFLAIVGSTLSGD
                     AVLKGRSPFGDRVDEVIAAPSITFVDDPTDARAFTAGTHDGEGLATRRNVLVDQGRLT
                     GFVHDAYSGRRSGAASTGSAVRSSRSTPMAGCQAISLLPGGRSQEELLAEVGEGLLVA
                     DVAGLHSGVNPVSGDFSTGADGLWIKDGQLAGPVREFTIASTLQRMLQDVVAVGRDLT
                     WLPMSAAGVTLVIGDVSVSGV"
     gene            900..2240
                     /locus_tag="VFP02_05190"
     CDS             900..2240
                     /locus_tag="VFP02_05190"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HET9542449.1"
                     /translation="MPDLGDLAQGIAARAEAGAELEAFVARSTSLSAKAYESEVESFT
                     SSESFGVGIRVIRDGRMGFASAGTFDQEVLDDTLAEARDNARFAEPDEFVGLVEPDGV
                     APPELDLWRDDLASTPTEAKVQMALDLEAAIRSADPRIIGVRTAAYGEASGEAAVASS
                     RGLVVGSRGTSCSLGASALAREGDDTQIGGGSTIGRGVADLDPDEAVVDAVLRATRLL
                     GATQPPTARVTIVLEPRLAATLLGIAGGTLTGDRVLKGRSPFADRVGEAIASPLLTLV
                     DDPTDRRSFGADRHDGEGLATRVNPLITDGVLQGFLHDGYTGRRSGTASTGSAVRGAR
                     STPAPGCQALVMVAGDRSPEDLLADVQLGLFVQSFNGLHSGVNPVSGDFSVGADGLMI
                     RAGAVAEPVRELTLASTLQRLLLGIVAVGDDLDWRSGGTAAATMVIGDVSMSGS"
     gene            complement(2244..>2494)
                     /locus_tag="VFP02_05195"
     CDS             complement(2244..>2494)
                     /locus_tag="VFP02_05195"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET9542450.1"
                     /translation="FLLGFATLTAATAFTLLKDGGTLVDEFGIADPLVGALFAFGSAL
                     LAIRWLVGYLKGHDLSIFAWYRFVVAGLTIALLATSTI"
BASE COUNT          319 a          911 c          920 g          344 t
ORIGIN      
        1 gactccatgg gcacctcggt cgtcgtgtcc tcgacgggga tccgctccgg tggctccgag
       61 acggtctgtg acctggtggc ttacgccctg gccgaggagg cgggggagac ccagaccggc
      121 ttcggcttct cggtcggccg ctcgccgggc gagttggacg tcgacgaggc cgccaaggac
      181 gcggtctacc gggccacccg tctcctgggc gcgaccaagc cgccgtccca gcgcctgacg
      241 gtggtgctgg accccttcgt caccgcccag ttcctggcca tcgtgggcag cacgctgtcg
      301 ggggacgccg tgctcaaggg gcgctcgccg ttcggcgacc gggtcgacga ggtcatcgcc
      361 gccccctcca tcaccttcgt cgacgacccc accgacgccc gggccttcac cgcggggacc
      421 cacgacggcg agggcctggc cacccgccgc aacgtcctcg tcgaccaggg acgactgacc
      481 ggcttcgtcc acgacgccta cagcggccgc cgctcgggcg cggcttcgac cggttcggcc
      541 gtgcgctcct cccgcagcac accgatggcc ggctgccagg ccatcagcct gctgccgggc
      601 ggccgctccc aggaggagct gctggccgag gtcggcgagg gcctgctggt ggccgacgtg
      661 gccgggctgc actccggtgt gaacccggtc agcggcgact tctcgaccgg agcggacggc
      721 ctctggataa aggacggcca gctggccggc cccgtgcggg agttcacgat cgcgtcgacc
      781 ctgcagcgca tgctccagga cgtggtggcc gttggccgcg atctgacctg gctgccgatg
      841 tccgcggccg gggtgaccct ggtcataggc gacgtgtcgg tctccggggt ctgacgccgt
      901 tgcccgacct gggggacctg gcccagggca tcgccgcccg ggccgaagcg ggggcggagc
      961 tggaggcctt cgtggcccgc agcacctccc tgtcggccaa ggcctacgag agtgaggtcg
     1021 aatcgttcac ctcgtcggag tccttcggcg tcggcatccg ggtcatccgc gacggtcgga
     1081 tgggcttcgc ctcggccggc accttcgacc aggaggtgct ggatgacacg ctggccgagg
     1141 cccgggacaa cgcccgtttc gccgagccgg acgagttcgt cggcctggtg gagccggatg
     1201 gtgtcgcccc gccggagctg gacctgtggc gcgacgacct cgccagcaca ccgaccgagg
     1261 cgaaggtgca gatggcgctc gacctcgagg ccgccatccg gtcggctgac ccgcgcatca
     1321 tcggggtgcg cacggcggcg tacggcgagg cgtcgggcga ggccgcggtg gcgagcagcc
     1381 ggggcctggt cgtcggctcc cggggcacgt cctgctcgct cggcgccagc gctctggccc
     1441 gggagggcga cgacacccag atcgggggcg gctccaccat cggccgcggg gtggcggacc
     1501 tcgatcccga cgaggccgta gtcgacgccg tcctgcgggc cacccgcctg ttgggggcca
     1561 cgcagccgcc caccgcccgc gtcaccatcg tgctcgagcc ccgcctggcc gcgaccctgc
     1621 tgggcatcgc cgggggcacg ctcaccggcg accgcgtgct gaagggccgc tcgccgttcg
     1681 ccgaccgggt gggggaggcc atcgcgtccc cgctgctcac tctcgtcgac gaccccaccg
     1741 atcgccgctc cttcggggcc gaccgccacg acggcgaggg cctggccacc cgggtcaacc
     1801 cgctgatcac cgacggcgtg ctgcagggct tcctccacga cggctacacc ggccgccggt
     1861 cggggacggc gtcgaccggc tcggccgtgc ggggcgcccg ctcgacgccc gctccgggct
     1921 gtcaggcgct ggtgatggtg gccggcgacc ggtcacccga ggacctgctg gccgacgtcc
     1981 agctggggct tttcgtccag tcgttcaacg gcctgcactc cggggtcaac ccggtgagcg
     2041 gcgacttctc cgtcggggcc gacgggctga tgatccgggc cggcgcagtc gccgagccgg
     2101 tccgcgagct caccctggcc tcgaccctgc aacgcctcct cctcggcatc gtggccgtgg
     2161 gcgacgacct cgactggcgc tccggcggga ccgccgccgc caccatggtc atcggcgacg
     2221 tctccatgag cggcagctga ccgctagatg gtggaggtgg ccaggagggc gatggtcagg
     2281 ccggcgacga cgaagcggta ccaggcgaag atcgacaggt cgtggccctt gaggtagccc
     2341 accagccacc ggatggcgag cagggcggac ccgaaggcga acagggcgcc caccagcggg
     2401 tcggcgatcc cgaactcgtc gaccagggtg ccgccgtcct tcagcagcgt gaaggcggtg
     2461 gcggcggtga gcgtggcgaa gccgaggagg aagc
//
LOCUS       DASTFV010000273         4166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1046470, whole genome shotgun sequence.
ACCESSION   DASTFV010000273 DASTFV010000000
VERSION     DASTFV010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046470"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..797)
                     /locus_tag="VFP02_05200"
     CDS             complement(180..797)
                     /locus_tag="VFP02_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9542451.1"
                     /translation="MTEPSASRGRIVAAARAFVTADGVEAVSMRKVAAEVGLAPTAIY
                     WHVGSREDLLNAVLDEMIADLPPIRARGTGPRDRIASLARGARQEFHDNVRTLQLATE
                     LGRGNELSFRAQVALAREMTAAGLDGDDAVAALRSILFLVGGFVMIEEEYRQREPVQI
                     GTRHLWADVDDESIDPAIRSAMTGPTDTDALFTYTLDRLLDAVLD"
     gene            847..1623
                     /locus_tag="VFP02_05205"
     CDS             847..1623
                     /locus_tag="VFP02_05205"
                     /inference="COORDINATES: protein motif:HMM:NF018066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HET9542452.1"
                     /translation="MGQIRYGAKVEQRSREIEATGAEIWSTAVTAVYETDPELIAAVL
                     PKPLEPADEPRVRLTITSVEMPGLPVFGAGWFGVAARHGDQLGEYPIFMPMTTEQSVL
                     GGRDTYGEPKKIADVWARRDGDAIDAGITRMGFTVCEIQGTVTETREPYEKTKRDFWF
                     KLSPSAETAGELDQDPLLVYGEKTEKARVHEGIDGEVVLKDSPLDPIADLVVRRVVDI
                     NWTERASTQVGRVIGPVPREDLVPFIHQRYDDLSVLGRKS"
     gene            1625..2881
                     /locus_tag="VFP02_05210"
     CDS             1625..2881
                     /locus_tag="VFP02_05210"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9542453.1"
                     /translation="MSDRYTIISADSHCGLPAAEYRPFLDQKYHRQFDDYLAEQQGSR
                     DEALKLNYDYIMGWETENAEGLRGAFDPEQRDKEMDADGVSAEVMFADADAITGMASP
                     PFGAGLGAGMIDDPELAYAGARAHNRFLVEMCSHSPERRGGIALVPIAHDIERGVEEV
                     EWLAGQPGIRGVMIPTMWRDRLPYNDAAYDPVWAACEAAGFPVHTHSGEAPREEMGEH
                     IGIYLAEVVWWATRPMWHLLFSGAFERFPGLKFVVTEAAAYWSTDMMWKWDQYLGGGH
                     TTKKMAALMKGKISKLPSDYFGSNIFIGASTMSPEEIRRRHVIGCDVVMWGTDYPHPE
                     GTWPHTQAKLQSDFVDVSVEDTRQLLGETAAVCYGFDLEALAPIAERVGPTPEDLHQD
                     PDLRTDPDEVANARWWKAEYDVQAPV"
     gene            2958..3836
                     /locus_tag="VFP02_05215"
     CDS             2958..3836
                     /locus_tag="VFP02_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9542454.1"
                     /translation="MEDLNGKVAVVTGGAAGIGRGIVEALLEEGARVVVGDIEAPVLE
                     ATMAELAGRGDVRGVRTDVADPVSFEALAEDVFATEGACHVLFNNAGVSSGGGGKPWE
                     QEANDWRWCFSVNVFGTANGVLSFVPRMIESGEPGVVVNTSSGDGGLAPVPYASVYAS
                     SKAAVSCFTEALAHQFASGETNLKAAIFYPGGGLLDTGLWTAARNRPDELARQKERAP
                     APGTTFGEFKEQLAAAGRPTDVVDLVELGRFAVAGVKAEKFIISHDLDRAGQLLHARA
                     DAIADAQLPPHMMEFL"
     gene            3934..>4166
                     /locus_tag="VFP02_05220"
     CDS             3934..>4166
                     /locus_tag="VFP02_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET9542455.1"
                     /translation="MSDRYAVISSDGHAGAELYEYRDYLASQWHDDFDAWAPTYVNPF
                     ADLQAPTAYRSWDSARRLEETEADGIVAEVLFP"
BASE COUNT          661 a         1446 c         1470 g          589 t
ORIGIN      
        1 atgcgcccca cctcgttgtc gatgcccttg gacgtccccg tgaccagcag cgtgagggcg
       61 aagatgagcg aggtggccac gatggcaatg aggaagcggc gtcgccgcca ctgaagatct
      121 cgcaggctga ggagccacat cggcgcgatt tctagtgcct cggcgacggc cccgcggcgt
      181 cagtcgagga cggcgtcgag gaggcggtcc agggtgtagg tgaagagagc gtcggtgtcg
      241 gtgggcccgg tcatggcgga ccggatggcg ggatcgatgg actcgtcgtc cacatcggcc
      301 cagaggtggc gggtgccgat ctggaccggc tcccgctggc ggtactcctc ctcgatcatc
      361 acgaagccgc cgaccaggaa caggatcgag cgcagggcgg ccacggcgtc gtcgccgtcc
      421 aagccggccg cggtcatctc gcgggcgagg gcgacctggg cccggaacga gagctcgttc
      481 ccccgcccca gctcggtcgc cagctgcagg gtgcggacgt tgtcgtggaa ctcctggcgg
      541 gcgccccgcg ccagcgaggc gatccggtcg cgggggccgg tgccccgggc ccggatcggg
      601 ggcaggtcgg cgatcatctc gtcgagcacg gcgttcagca ggtcctcccg gctgcccacg
      661 tgccagtaga tggcggtggg ggcgaggccg acctcggccg ccaccttccg catgctcacg
      721 gcctcgaccc cgtcggccgt cacgaaggcg cgggccgcgg ccacgatgcg tccacggctt
      781 gccgacggct cggtcatctg taacagtgtt gcagagaccc gccgaggtgt cgacaagggg
      841 gatgtcatgg gccagatccg ctacggcgcg aaggtcgagc agcgcagtcg ggagatcgag
      901 gccaccgggg ccgagatctg gtccacggcg gtcacggccg tctacgagac cgatcccgag
      961 ctgatcgccg ccgtcctccc caagccgctc gaaccggcgg acgagccgcg ggtgcgcctg
     1021 accatcacct cggtcgagat gcccggcttg cccgtgttcg gtgccgggtg gttcggtgtg
     1081 gccgcccgcc acggcgacca gctcggcgag tacccgatct tcatgcccat gaccaccgag
     1141 cagtcggtgc tcgggggccg ggacacctac ggcgagccca agaagatcgc cgacgtctgg
     1201 gcccgccgcg acggcgacgc catcgacgcc ggcatcaccc ggatgggctt caccgtctgt
     1261 gagatccagg gcacggtcac cgagacccgg gagccctacg agaagaccaa gcgcgacttc
     1321 tggttcaagc tctcaccgtc ggccgagacg gccggcgagc tcgaccagga cccgctcctg
     1381 gtctacggcg agaagaccga gaaggcccgt gtccacgagg gcatcgacgg cgaggtcgtc
     1441 ctgaaggact cgccgctcga ccccatcgcc gacctcgtcg tgcgccgggt cgtggacatc
     1501 aactggaccg agcgggcctc cacccaggtc ggccgggtca tcggccccgt gccccgggag
     1561 gacctggtcc ccttcatcca ccagcgctac gacgacctgt ccgtcctcgg gaggaagagc
     1621 tgagatgagc gaccgttaca cgatcatctc ggccgactcc cactgcgggc tcccggcggc
     1681 cgagtaccgg cctttcctcg accagaagta tcaccggcag ttcgacgact acctggccga
     1741 gcagcagggc tctcgggacg aggccctgaa gctcaactac gactacatca tgggctggga
     1801 gaccgagaac gccgagggtc tccggggggc cttcgacccc gagcagcggg acaaggagat
     1861 ggacgccgac ggcgtctccg ccgaggtcat gttcgccgac gccgacgcca tcaccggcat
     1921 ggcctccccg cccttcgggg ccggcctggg ggccgggatg atcgacgacc cggagctggc
     1981 ctatgccggc gcccgggccc acaaccgctt tctggtcgag atgtgcagcc acagcccgga
     2041 gcgccgaggc ggcatcgccc tggtccccat cgcccacgac atcgagcggg gggtcgagga
     2101 ggtcgagtgg ctggccggcc agcccggcat ccggggcgtg atgatcccga ccatgtggcg
     2161 ggaccgactg ccctacaacg acgccgccta cgacccggtc tgggccgcct gcgaggccgc
     2221 cgggttcccg gtccacaccc actccggcga ggctcctcgc gaggagatgg gcgagcacat
     2281 cggtatctac ctggccgagg tcgtctggtg ggccacccgg ccgatgtggc acctcctgtt
     2341 ctccggcgcc ttcgagcgct tccccggcct gaagttcgtg gtcaccgagg ccgccgccta
     2401 ctggtccacc gacatgatgt ggaagtggga ccagtacctg gggggcgggc acacgaccaa
     2461 gaagatggcc gccctgatga agggcaagat ctccaagctg ccgtcggact acttcggctc
     2521 gaacatcttc atcggggcct cgaccatgtc gcccgaggag atccggcgcc gtcacgtcat
     2581 cggctgtgac gtcgtgatgt ggggcacgga ctacccgcac ccggaaggga cctggcccca
     2641 cacacaggcc aagctgcagt ccgacttcgt ggacgtctcc gtcgaggaca cccgccagct
     2701 gctcggggag acggcggcgg tctgttacgg gttcgacctc gaagccctcg ccccgatcgc
     2761 cgaacgggtc gggccgacgc ccgaggacct ccaccaggac cccgacctgc gcaccgatcc
     2821 cgacgaggtg gccaacgccc gttggtggaa ggccgagtac gacgtgcagg ccccggtgtg
     2881 aacgctcccc ttcgcgaacc gtgtgcacag ctctacgagg gctgtagcgc cgcgcacaag
     2941 gttcgaggag gtccgtgatg gaggacctga acgggaaggt cgcggtcgtc acaggcggcg
     3001 cggcggggat cgggcggggg atcgtcgaag cgctgctcga ggagggggcg cgggtcgtcg
     3061 tcggtgacat cgaggccccg gtgctcgagg ccaccatggc cgagctggcc ggacggggcg
     3121 acgtgcgcgg ggtgcggacc gacgtggccg acccggtctc gttcgaggcc ttggccgagg
     3181 acgtgttcgc caccgaaggc gcctgccacg tgctcttcaa caacgccggg gtgagctcgg
     3241 gcgggggtgg caagccgtgg gagcaggagg ccaacgactg gcgctggtgc ttcagcgtca
     3301 acgtgttcgg caccgccaac ggcgtgcttt ccttcgtgcc ccgcatgatc gagtccggcg
     3361 agccgggcgt cgtcgtcaac acctcgtcgg gcgacggtgg gctcgcccct gtgccctacg
     3421 cgtcggtgta cgcgtcgtcc aaggcggcgg tgagctgctt caccgaggcc ctggcccacc
     3481 agttcgccag cggcgagacc aacctgaagg cggcgatctt ctacccgggc ggggggctgc
     3541 tcgacaccgg actctggacg gccgcgcgca accgacccga cgagctggcc cgccagaagg
     3601 agcgggcgcc ggccccgggc acgaccttcg gcgagttcaa ggagcagctg gccgccgccg
     3661 gccgcccgac cgacgtggtc gacctggtgg agctgggccg cttcgccgtg gccggcgtga
     3721 aggccgagaa gttcatcatc agccacgacc tcgaccgagc cggtcagctc ctgcacgccc
     3781 gcgccgacgc catcgccgac gcccaactac ccccgcacat gatggagttc ctgtgaccat
     3841 gggtgacgct gcgcagcgga gcggagaagc tccgccgatt tcggagatgg cgaagccagc
     3901 cgggcggagc acggcgagtg tgatggagtt cttgtgagcg accgatacgc cgtcatctcc
     3961 tcggacggcc acgccggggc cgagctctac gagtaccggg actacctcgc ctcgcagtgg
     4021 cacgacgact tcgacgcctg ggccccgacc tacgtcaatc cgttcgccga cctccaggcc
     4081 ccgaccgcgt accgcagctg ggactcggcc cgccgcctgg aggagaccga agcggacggc
     4141 atcgtggccg aggtcctctt cccgaa
//
LOCUS       DASTFV010000274         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1049380, whole genome shotgun sequence.
ACCESSION   DASTFV010000274 DASTFV010000000
VERSION     DASTFV010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049380"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..549)
                     /locus_tag="VFP02_05225"
     CDS             complement(97..549)
                     /locus_tag="VFP02_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542456.1"
                     /translation="MELFEEACEALRGLVPPELGPFRHKTHRYGTKIWFGPEAPPKEH
                     FEAQVVGVRDVPEAEVLAIEVGFHSEYPKPAENDEVLAALLAAEGEWRPDLGDEAVAG
                     EFLGRADNWRRISETWLDPDLDDPELGTELAMRLLDYIEALEPIRAGR"
     gene            complement(551..>2459)
                     /gene="pheT"
                     /locus_tag="VFP02_05230"
     CDS             complement(551..>2459)
                     /gene="pheT"
                     /locus_tag="VFP02_05230"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET9542457.1"
                     /translation="LGLQGDWLLDLEVNANRPDALSMVGVARDVAARLGVGFTLPSGT
                     APTGGASSRDVASVEIVDGAACPRFVARVLRDVTIGPSPTWLANRITQAGMRPISNVV
                     DVSNYVMLELGHPNHAYDLDKVAGGRLRVRFAHDGEQLVTLDDVERSFLVADVLICDG
                     EDTPIGVAGVMGGASTEISDTTTKVLLEAAWWHPMQIARTSTRLNLRSEASRRFERGT
                     DIWGLDLAIERFCSLLAETGAVAAPDVIDERGDVPEHPPIRVRTDRVNLITGLDLDAP
                     TIRGLLVPIGFTVGEEADGAQVVDVPSFRPDVVEEIDVIEEVARHHGYAKLPRTVPLS
                     PHTGSLTERQKERRRIRATLVGLGLDEALPMPFLAPEDLARTGVADHGITVTNPLDAR
                     ESIVRPSLRPGLLRALAYNASHRNDEAGLFEIGKVFLPVADGEERPLEPEHLGVALAG
                     REAPEAVAVWTAVAEALSLDGIRLEAVEMAGLHPTRSAQLVTDAGPVGVVGEIDPAVA
                     DAWGIGERVAWLEVGLDDLAEVPRRDRLYRPVSRFPSNDIDLAFVVDEVVPAAAVETT
                     IRQSAGELLARLRLFDVFRGDAVGDGRRSLAYALRLQATDRTLKDGEVAEIRQGVIDA
                     VESTHGASLRG"
BASE COUNT          349 a          870 c          894 g          346 t
ORIGIN      
        1 acccggacgc tccgacgatc cccacacgca tgcggttgac tatacgtgat tacgcatgac
       61 tatacaatca gccgtcgtcg agaccagccg gcccggtcat cgaccggcgc gaatcggctc
      121 cagggcttcg atgtagtcga gcaacctcat cgccagctcg gtgccgagct cggggtcgtc
      181 gaggtcgggg tcgagccagg tctcggagat gcgccgccag ttgtcggcgc ggccgaggaa
      241 ctcgccggcc acggcctcgt cgcccaggtc cggccgccac tccccctcgg ccgccagcag
      301 ggcggcgagc acctcgtcgt tctcggcggg cttggggtac tcgctgtgaa agccgacctc
      361 gatggccagg acctcggcct cgggcacgtc ccggacgccg acgacctggg cctcgaagtg
      421 ctccttgggc ggggcctcgg gaccgaacca gatcttggtg ccgtaacggt gggtcttgtg
      481 ccggaagggg cccagctcgg gcgggacgag gcctcggagg gcttcgcagg cctcctcgaa
      541 gagctccacc tcagccccgg aggctggccc cgtgggtgga ctcgacggcg tcgatgaccc
      601 cctggcggat ctcggccacc tcgccgtcct tcagcgtgcg gtctgtggcc tggagacgca
      661 gggcgtaggc caggctgcgc cggccgtcgc cgacggcatc cccccggaag acgtcgaaca
      721 ggcggagccg ggccagcagc tcgcctgcgg actgccggat ggtggtctcc accgccgcgg
      781 cgggcacgac ctcgtcgacc acgaacgcca ggtcgatgtc gttggaagga aagcggctga
      841 cgggtcggta gagccggtcc cgccggggca cctcggccag gtcgtccagg cccacctcca
      901 gccacgccac ccgctcgccg atgccccagg cgtccgcgac cgccgggtcg atctcgccca
      961 ccacgccgac cgggccggcg tcggtcacca gctgtgcgct gcgagtgggg tgcagtcctg
     1021 ccatctccac ggcctccagc ctgatgccgt ccagcgagag ggcttcggcc accgccgtcc
     1081 agaccgcgac ggcctcaggg gcctcccgcc cggcgagggc gaccccgagg tgctcgggct
     1141 cgagcgggcg ctcctcgccg tcggccacgg ggaggaagac cttgccgatc tcgaacaggc
     1201 ccgcctcgtc attgcggtga ctggcgttgt aggccagcgc tcgcagcagg ccggggcgga
     1261 gcgacgggcg cacgatggat tcccgggcgt cgagcgggtt cgtcacggtg atgccgtggt
     1321 cggccacgcc ggtgcgggcc aggtcctccg gcgccaggaa cggcatgggc agggcctcgt
     1381 ccaggccgag gccgaccagg gtggctcgga tccggcgccg ctccttctgg cgctcggtca
     1441 ggctcccggt gtgcggcgac agcggcaccg tccggggcag tttggcgtag ccgtggtggc
     1501 gggcgacctc ctcgatgacg tcgatctcct cgacgacgtc cgggcggaag ctggggacgt
     1561 cgacgacctg ggcgccgtcg gcctcctcgc ccaccgtgaa gccgatgggc accaggagcc
     1621 cccggatcgt cggggcatcc aggtcgaggc cggtgatgag gttgacccgg tcggtccgca
     1681 cccggatcgg cgggtgctcg gggacatcgc cccgctcgtc gatgacgtcg ggggccgcca
     1741 ccgcgccggt ctcggccagc agtgagcaga agcgctcgat ggcgaggtcc aggccccaga
     1801 tgtcggtccc ccgctcgaag cggcgggagg cctccgagcg caggttgagc cgggtcgacg
     1861 tgcgggcgat ctgcatcggg tgccaccagg ccgcctcgag cagcactttg gtggtggtgt
     1921 cgctgatctc ggtcgacgct ccacccatga caccggcgac gccgatgggc gtgtcctcac
     1981 cgtcgcagat caggacgtcc gcgaccagga acgagcgctc gacgtcgtcg agggtgacca
     2041 gctgctcccc gtcgtgggca aagcggaccc gtaggcgacc accggccacc ttgtccaggt
     2101 cgtaggcatg gttggggtga cccagctcga gcatgacgta gttggagacg tcgaccacat
     2161 tgctgatggg ccgcatgccc gcctgggtga tgcggttggc cagccaggtc ggcgacgggc
     2221 cgatggtcac gtcccgcaac acgcgggcga cgaagcgggg gcaggcggcg ccgtccacga
     2281 tctcgaccga agcgacatcc cggctggagg cgccacctgt cggcgcggtc ccggagggca
     2341 gggtgaagcc cacaccgagc cgagcggcca cgtcccgggc cacgccgacc atcgacagcg
     2401 catcgggccg gttggcgttg acctcgaggt cgagcagcca gtccccctgc aggcccagc
//
LOCUS       DASTFV010000275         2399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1051684, whole genome shotgun sequence.
ACCESSION   DASTFV010000275 DASTFV010000000
VERSION     DASTFV010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051684"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="VFP02_05235"
     CDS             complement(<1..1275)
                     /locus_tag="VFP02_05235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9542458.1"
                     /translation="MSNRRFALALAGIALLGLGLRWFWILEMRPECGDPAEDGPPGCL
                     AITGGANVVNDPRYLHEVGNLLAEGKPFIDPFTYASTGEERPSAHKMPLFPMYLGLVS
                     ALGGHGVDTHRLAAALLGALTVVVLGLVGRDLLGWRAGLIAAAIGALYPNLWINDFLL
                     QVESLATLLIALTILAAYRYRGDPTRWRIALLGLVIALTALTRAESLLLYALVALPLI
                     LGAVRTWRERLLHVALAGAAAVLLLGPWVAWNLTRFEEPTTLSTAPGPVLDTASCDAT
                     YYGEQLGFYGSCFVIEDYAADEGLTVPEAQERLRGYDESQRAAVAGERAQIYIRDHLG
                     RLPVVMVARVARMWDFYRPLQNVRFNWQLEARGERTSEVALLTYYALVPFAVGGVVAL
                     KRRGLPISPLVGMAVMISVTAAWTFGTTRYRIP"
     gene            complement(1272..>2399)
                     /locus_tag="VFP02_05240"
     CDS             complement(1272..>2399)
                     /locus_tag="VFP02_05240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9542459.1"
                     /translation="WRDSIEAGGPPVYEPSAGHPPGYVLYLAAYGVIGLDSPLANRLA
                     SGIAGAAAVALIGITARMIGRWAGLGERGGDRAGLLAAFLAAIYPNLWINDALILSES
                     LYAGLTVLVILAGYRFWRHPSWRSAAVLGGAVGIATLTRSEGLLLLPGLVLPLAVMLR
                     DRTWGQRAGLAIVSGTVALALMAPWMISNLLRFEEPAPPYLATGSGRVLAFGNCDRTY
                     SGDFFGYWNIDCAPAEFSGDESQIDTIHREKATAYMEDHLGDLPRVALARVGRMWQVY
                     RTDQGVEFDRFFERRGLDETRFALGQYYLMVPIAVAGVVLLWRRRVTIIPLVSPFVLV
                     TFTAATTFGITRYRVPSEVALCMLAGLGLAWVTLRDAPVEA"
BASE COUNT          398 a          815 c          896 g          290 t
ORIGIN      
        1 cgggatccgg taccgggtcg tcccgaaggt ccacgccgcg gtgaccgaga tcatcaccgc
       61 catccccacg agcgggctga tgggcagacc ccgccgcttg agggcgacca cgccgcccac
      121 cgcgaacggg acgagggcgt agtaggtgag caaggccacc tccgaggtcc gctcgccccg
      181 agcctccagc tgccagttga agcggacgtt ctgcagtggg cggtagaagt cccacatccg
      241 ggccacccgg gcgaccatca cgacggggag gcgaccgagg tggtcccgga tgtagatctg
      301 ggcccgctcc cccgccaccg ccgcccgctg ggattcgtcg tagccccgaa gccgttcctg
      361 ggcctcgggc acggtcagtc cctcgtcggc tgcgtagtcc tcgatcacga agcagctgcc
      421 gtagaagccg agctgctcgc cgtagtaggt ggcgtcgcag ctggccgtgt ccagcaccgg
      481 accgggggcc gtggacaggg tggtcggctc ctcgaagcgg gtcaggttcc aggccaccca
      541 ggggcccagg aggaggaccg cggcggcccc ggcgagggcc acgtgcagga ggcgctcccg
      601 ccaggtccgc acggcgccga ggatcagcgg cagggcgacg agggcgtaca gcagcagcga
      661 ctcggcccgg gtgagcgcgg tcagcgcgat cacgaggccg aggagggcga tccgccatcg
      721 ggtcgggtcc ccccggtacc ggtacgcggc caggatcgtg agcgcgatca gcagggtggc
      781 caggctctcc acctggagca ggaagtcgtt gatccagagg ttcgggtaga gggccccgat
      841 ggcggcggcg atgagcccgg cccgccaacc caggaggtcc cgcccgacca ggcccaggac
      901 cacgacggtg agcgcgccca ggagggcggc ggccagccga tgggtgtcga cgccgtgtcc
      961 gccgagagcc gagaccagcc cgaggtacat cgggaacagc ggcatcttgt gggcgctggg
     1021 tcgttcctca cccgtgctgg cgtaggtgaa cgggtcgatg aagggcttgc cctcggccag
     1081 caggttcccc acctcgtgca ggtagcgggg gtcgttgacc acgttggccc cgccggtgat
     1141 ggccaggcac cccggcggtc cgtcctcggc cgggtcgccg cactccgggc gcatctccag
     1201 gatccagaac cagcgcaggc ccaggcccag caaggcgatg ccggccaggg ccagggcgaa
     1261 gcgtcggttg ctcacgcctc gacgggggcg tcccgcaggg tcacccaggc cagtcccagg
     1321 ccggcgagca tgcagagcgc gacctccgag gggacccggt accgggtgat gccgaaggtg
     1381 gtggcggcgg tgaaggtgac cagcacgaac ggcgagacca gggggatgat cgtcactcgc
     1441 cgccgccaca ggaggacgac cccggccacc gcgatcggca ccatcaggta gtactgaccg
     1501 agggcgaacc gcgtctcgtc caggccccga cgttcgaaga accggtcgaa ctcgaccccc
     1561 tggtcggtgc ggtacacctg ccacatccgt ccgacacggg cgagggccac gcggggcaga
     1621 tcaccgagat ggtcctccat gtaggcggtg gccttctcgc gatggatggt gtcgatctgg
     1681 gactcgtcgc cggagaactc ggcgggggcg cagtcgatgt tccagtagcc gaagaagtcc
     1741 ccggagtagg tcctgtcgca gttgccgaag gccagcaccc ggccactgcc ggtcgccagg
     1801 tagggcggcg ccggctcctc gaagcgcagg aggttgctga tcatccaggg ggccatcagg
     1861 gcgagggcga ccgtgcccga cacgatggcc aaaccggccc gttggcccca ggtgcggtcg
     1921 cggagcatga cggcgagggg caggaccagg cccggcagga ggagaaggcc ctcgctgcgg
     1981 gtgagggtgg cgatgcccac ggccccgccg aggacggctg cgctccgcca cgacggatgc
     2041 cgccagaagc gatagccggc caggatcacc aacaccgtga gcccggcgta gagcgactcg
     2101 gagaggatga gcgcgtcgtt gatccagagg ttggggtaga tggcagccag gaaggcggcc
     2161 aagagcccgg cgcggtcacc gccccgctcc cccaaccctg cccaccggcc gatcatgcgg
     2221 gcggtgatcc cgatgagggc gacggcggct gcgccggcga tgccgctggc cagccggttg
     2281 gcgagcgggc tgtccaaccc gatcacgccg taagcggcca ggtagagcac gtagcccggc
     2341 ggatgaccgg cggagggctc gtagaccggg ggcccaccgg cctcgatcga gtcccgcca
//
LOCUS       DASTFV010000276         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1055870, whole genome shotgun sequence.
ACCESSION   DASTFV010000276 DASTFV010000000
VERSION     DASTFV010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055870"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VFP02_05245"
     CDS             <1..828
                     /locus_tag="VFP02_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542460.1"
                     /translation="IRLKWDPRRLSRRGLIGLAAFIWIVIKLPQEWWIHVAQLDFTDF
                     MKEDVFGVPVATPWGEAIAENLWFVGLLAMVVVGAALVWRSQRAKVPTPDWPTTVRVA
                     DHLEPVPSDTQGARDGILSLPTGEKVALIGLICVIFASVLPDIDRGPLQTAVGVGLLI
                     VANATVSHWLTGRGVRWRSTGTQLLTMLVVNASLLWLFGTLGRASREDLNAGALVFFT
                     LVISLLVTLYDRFEAEREARLADGTTSAGEGSSPVERSGIGLTTPRSAPGSRPRRPA"
     gene            complement(779..1231)
                     /locus_tag="VFP02_05250"
     CDS             complement(779..1231)
                     /locus_tag="VFP02_05250"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HET9542461.1"
                     /translation="MRRLMALVLFAVLLAAGCGDDSGGDDASGDDSSGGGTPATAEDV
                     DGQTFASTGSTGHELVADTVVSLTFTDGTVSFSAGCNSMNAGYELTDGTLELTSEVAG
                     TLMACPPDLQEQDEWLSGFMADGPTATVDGDVLTLVAGDETLELTAAS"
     gene            complement(1324..>2514)
                     /locus_tag="VFP02_05255"
     CDS             complement(1324..>2514)
                     /locus_tag="VFP02_05255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9542462.1"
                     /translation="PLALIGARALLGVAGATLMPSTLSLIRNIFMDDRRRQFAVAVWA
                     TMFSVGAVVGPIIGGLLLEHYWWGSVFLVGVPVTTALLLIGPRVIPESRDPDPGPFDV
                     LSSALSMATILPLVFGMKTVAERGWATVVAPSVVIGLVAGWAFVRRQRVLAVPMIDLS
                     LFAVPKFRVAITGNLLTCVGFAGSTFFVTQYLQLVLGLSPTRAALQLLPGAAAGVIST
                     MSAPAMTRRFGTFAVITTGLATGAFGFLLLTQLTVGSSLVPVTVALVIIEGGLCAAIT
                     VAIDAIISSVPPEKAGAGASVSETANELGVALGTAVLGSILTATYRRQLDDIAVPAVA
                     LDEARETLGAAHVAASDLGGPMGDALRSAADVAFTDGLRLASAVAAVLLAAVSIASWR
                     VSHR"
BASE COUNT          393 a          880 c          910 g          331 t
ORIGIN      
        1 atccggctga agtgggaccc ccgccgcctg tcgcgccgcg ggctcatcgg tctcgccgcc
       61 ttcatctgga tcgtcatcaa gctcccgcag gagtggtgga tccacgtggc ccaactggac
      121 ttcacggact tcatgaagga ggacgtgttc ggtgtgccgg tcgccacccc ctggggcgag
      181 gccatagccg agaacctctg gttcgtcggc ctgctggcaa tggtcgtcgt cggcgccgcc
      241 ctggtgtggc gaagccagcg ggccaaggtg ccgacgcccg actggccgac gaccgtccgg
      301 gtcgccgacc acctcgagcc ggtgccgtcg gacacgcagg gggcccggga cgggatcctg
      361 tccttgccga ccggcgagaa ggtggcgctc atcggtctca tctgcgtgat cttcgcctcg
      421 gtgctgcccg acatcgaccg gggccccctg cagaccgcgg tcggggtcgg cctgctgatc
      481 gtcgccaacg cgacggtgag tcactggctg acgggtcggg gggtccggtg gcgttccacc
      541 ggcacgcagt tgctcaccat gctcgtggtc aacgccagcc tgctctggct gttcggaacc
      601 ctcggtcgcg catcccgcga ggacctgaac gctggcgcgc tggtcttctt caccctggtc
      661 atcagcctgc tggtgaccct gtacgaccgg ttcgaggccg aacgcgaggc ccggctcgcc
      721 gatggcacga cctcggcggg cgagggcagc tcgccggtcg agaggagcgg gatcggactc
      781 acgacgccgc ggtcagctcc agggtctcgt ccccggcgac cagcgtgagc acgtcaccgt
      841 ccacggtggc cgtgggaccg tccgccatga agcccgagag ccactcgtcc tgctcctgca
      901 ggtccggcgg gcacgccatc agggtcccgg ccacctcgga ggtcagctcg agggtcccgt
      961 cggtcagctc gtagccggcg ttcatgctgt tgcagccggc cgagaacgag acggtcccgt
     1021 cggtgaaggt caggctgacc acggtgtcgg cgaccaactc gtggccggtg ctcccggtcg
     1081 acgcgaacgt ctgaccgtcg acgtcctccg ccgtcgccgg ggtgccacca ccggagctgt
     1141 cgtcaccgga ggcatcgtcg cccccgctgt cgtcaccgca ccctgcggcc agcaggaccg
     1201 cgaacaggac aagcgccatg agccgtcgca tggccgccga agctagcccc acccccgctc
     1261 cccatcggtt ctggcccctg aacctcgcgt tgggccgcga ccttcagggg ccacaacggt
     1321 gggtcagcga tgggagacgc gccagctggc gatgctgacc gcggccagca agacggcggc
     1381 caccgcggac gccaaccgga ggccgtcggt gaaggcgacg tcggcggcgg accggagggc
     1441 gtcacccatc ggcccaccga ggtcactcgc cgccacgtgc gccgccccga gggtctcgcg
     1501 ggcctcgtcc agggcgaccg cggggacggc gatgtcgtcg agctgacgcc ggtaggtggc
     1561 ggtgaggatg ctccccagca ccgcggtgcc gagggccacg cccagctcgt tggcggtctc
     1621 tgacaccgag gccccggccc ccgccttctc aggggggact gagctgatga tggcgtcgat
     1681 ggccacggtg atggccgcgc agagcccgcc ttcgatgatc accagggcga cggtcaccgg
     1741 caccagcgag ctgcccacgg tgagctgcgt caggaggaga aagccgaagg ccccggtggc
     1801 cagcccggtc gtgatgacgg cgaaggtgcc gaaccgccgg gtcatggccg gcgccgacat
     1861 cgtcgagatc acgccggcgg ccgcccccgg caggagctgc agggcggccc gggtggggga
     1921 gaggcccagg accagctgga ggtactgggt gacgaagaac gtcgagccgg cgaagccgac
     1981 gcacgtcagc aggttgccgg tgatggccac ccggaacttg ggcacggcga acagcgacag
     2041 gtcgatcatc ggcacggcca ggacgcgttg gcggcgcacg aaggcccagc ccgcgacgag
     2101 gccgatgacc acggacgggg ccacgaccgt cgcccaaccc cgctcggcca ccgtcttcat
     2161 gccgaacacc agcgggagga tggtggccat ggacagcgcc gagctgagga cgtcgaaggg
     2221 gccggggtcg gggtcgcggg actcggggat cacccggggg ccgatcagga gcagggcggt
     2281 ggtgacgggc acccccacga ggaagaccga gccccaccag tagtgctcca gcaggaggcc
     2341 accgatgatg gggccgacga cggcgccgac cgagaacatc gtggcccaga cggcgaccgc
     2401 gaactggcgc cgccggtcgt ccatgaagat gttgcggatc agcgagaggg tcgacggcat
     2461 cagcgtcgcc ccggccacgc ccaacagggc ccgagccccg atcagagcca gcgg
//
LOCUS       DASTFV010000277         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1057357, whole genome shotgun sequence.
ACCESSION   DASTFV010000277 DASTFV010000000
VERSION     DASTFV010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057357"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFP02_05260"
     CDS             <1..288
                     /locus_tag="VFP02_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9542463.1"
                     /translation="TEDGEVTAVDATTGDVVWATEVPGDPLGGTAVVNDLVFTALLDG
                     TVLALDRATGDIVWTFAAGGGVNGWMSVAGDEIFVPVGNAAPPQLLTLRLG"
     gene            complement(307..1944)
                     /locus_tag="VFP02_05265"
     CDS             complement(307..1944)
                     /locus_tag="VFP02_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9542464.1"
                     /translation="MTSPSPWVRDEQHTVLDLLRARVEASPDDPYLDVIGEALTAGEV
                     SRSAARIGGTLRALGVRPGDRVASLIENSAEAVLVWFGILWAGGVAVPVNTAYKGEYL
                     RHQLTDCGARALVVQADLAERAERVVPEVAAIEHVVVIDGEPGVGRFGSATTHAWADL
                     LDGPEGEPVAAAPSDLATFVYTGGTTGPSKGCMLSHNYHQVLARQIGTCWRRTADDVV
                     WTPLPMFHFNAIVTAVVGSLLFGGSGAISSRFSVSNFWPEMNRTGATITSTLGTMAYL
                     LAHDEDRPEMPLAGGPEANTSLRLLGAAPMPEEVDRIIRSRFGIDTFSGAYGLTEASL
                     VSWQPPGVDNKANAAGVINDEYFDVRVFDDDDHELPRGTAGEIVLRPKRPQSMFEGYW
                     GRPEATVEASRNWWFHTGDIGRIDEDDFLFFVDRKADYLRRRGENISSFEVERILMGH
                     GALADVVVHAVPSELGEDDLKISATLRADATLREEDLFRWCQDELPYFVLPRYIEFRE
                     ELPRNPVGRVLKRTLRDEGVTADTWSVDDAGIEIEKR"
     gene            complement(1941..>3088)
                     /locus_tag="VFP02_05270"
     CDS             complement(1941..>3088)
                     /locus_tag="VFP02_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HET9542465.1"
                     /translation="HVAESYGDDNPLWCDPAYAATSRWGGVIAPPHLVGGDPLIGENE
                     VTGYDDETKALMKGDPLRGVHAFYSGSHREWWQPLYPGARIARRNALVGVHDKAGEFA
                     GRAVHEWTAEVFCQQDGPPLAAQYRLMIRGEREKAEASGKYDDTEIRPYTDEEIERIT
                     EAVLAEGDRRRGAEPRWWEEVAEGDELEPLVKGPFRVTDIVCWHVGMGMGLYGVKALR
                     LGQEQRRKVPRFFRPDDLNVPDVQQRVHWDAEWARRAGSPAIYDYGRMRETWLIHACT
                     DWMGDDAWLWKLDCQFRRFNYVGDTHWVRGRITRRYLTDWGQPAVDLDLWGENQRGET
                     TSPGHASILLPSRESGEVRLPPVPGGAGTAQEVLDALVERFADEEAS"
BASE COUNT          413 a         1084 c         1125 g          466 t
ORIGIN      
        1 accgaggacg gcgaggtcac cgccgtcgac gcgaccacgg gcgacgtcgt ctgggccacc
       61 gaggtccccg gtgaccccct tggggggacc gcagtggtga acgacctcgt gttcacggcg
      121 ctgctggacg ggacggtgct ggccctggac cgggcgaccg gcgacatcgt ctggaccttc
      181 gccgcgggtg gcggcgtcaa cggctggatg tcggtggccg gcgacgagat cttcgtcccc
      241 gtcggcaacg ccgccccgcc gcagttgctc acgctgcgcc tcggctgacg agggcgaagc
      301 ggttggtcag cgcttctcga tctcgatccc ggcgtcgtcg acggaccacg tgtctgcggt
      361 gaccccttcg tcccgcaggg ttcgcttcag gacccggccc accgggttgc ggggcaactc
      421 ctctcggaac tcgatgtagc gggggagcac gaagtagggg agctcgtcct ggcaccagcg
      481 aaagagatcc tcctcgcgga gcgtggcatc ggcccgcagg gtggcgctga tcttcaggtc
      541 gtcctcgccc agctcggacg gcacggcgtg gaccacgacg tcggccaggg cgccgtgccc
      601 catcaggatg cgctccacct cgaagctgga gatgttctcg ccccggcggc gcaggtagtc
      661 ggccttgcgg tccacgaaga acaggaagtc gtcctcgtcg atgcggccga tgtcgccggt
      721 gtggaaccac cagttgcggc tggcctcgac ggtggcctcg ggccgacccc agtagccctc
      781 gaacatggac tgcggccgct tgggccgcag cacgatctcg ccggcggtgc cccggggcag
      841 ctcgtggtcg tcgtcgtcga agacgcggac gtcgaagtac tcgtcgttga tgacccccgc
      901 ggcgttcgcc ttgttgtcca cgcccggtgg ttgccacgac accagcgagg cctcggtcag
      961 gccgtaggcc ccgctgaagg tgtcgatgcc gaagcggctg cggatgatgc ggtcgacctc
     1021 ctcgggcatc ggggccgccc cgaggaggcg cagggaggtg ttggcctcgg ggccgcccgc
     1081 caggggcatc tccgggcggt cctcgtcgtg ggccaggagg taggccatgg tcccgagggt
     1141 cgaggtgatc gtggcgccgg tccggttcat ctccggccag aagttggaca ccgagaagcg
     1201 gctggagatg gcaccggagc cgccgaacag gagtgagccg acgacggcgg tgacgatggc
     1261 gttgaagtgg aacatgggca acggcgtcca gaccacgtcg tcggccgtgc gcctccagca
     1321 ggtcccgatc tggcgggcca ggacctggtg gtagttgtgg ctgagcatgc agcccttgga
     1381 cggtccggtc gtgccgcccg tgtagacgaa cgtggccagg tcgctcgggg cggcggcgac
     1441 cggctcgccc tccgggccgt cgaggaggtc ggcccaagcg tgggtcgtgg ccgagccgaa
     1501 gcgaccgact ccgggctcgc cgtcgatgac gacgacgtgc tcgatggccg ccacctccgg
     1561 gaccacccgc tcggcccgct cagccaggtc ggcctgcaca acgagggccc gagccccgca
     1621 gtcggtgagc tggtggcgga ggtactcgcc cttgtaggcg gtgttgacgg ggacggccac
     1681 gccgccggcc cacaggatcc cgaaccagac gagcacggcc tcggccgagt tctcgatgag
     1741 cgaggcgacc cggtcgccgg gccggacacc gagggcccgc agggtcccgc cgatgcgggc
     1801 cgcgctccgg ctgacctcgc cggcggtcag ggcctcgccg atgacgtcca gataggggtc
     1861 gtcgggcgac gcctcgaccc gggcccgcag caggtccagc acagtgtgct gctcgtcgcg
     1921 gacccacggg gacggtgagg tcacgaggcc tcctcgtcgg cgaagcgctc gacgagggcg
     1981 tcgagcacct cctgggccgt gcccgctcct ccgggcaccg gcggcagccg gacctccccg
     2041 ctctcgcggc tgggcagcag gatcgaggcg tgaccggggc tggtcgtctc gcctcgttgg
     2101 ttctcgcccc acaggtcgag gtcgacggcg ggttggcccc agtcggtcag gtagcgccgg
     2161 gtgatgcggc cccgcaccca gtgggtgtcg ccgacgtagt tgaagcggcg gaactggcag
     2221 tccagcttcc agagccaggc gtcgtcgccc atccagtcgg tgcaggcgtg gatcagccac
     2281 gtctcccgca tccgtccgta gtcgtagatg gcggggctgc ccgcccgccg ggcccactcg
     2341 gcgtcccagt gcacgcgctg ctggacatcg gggacgttga ggtcgtcggg ccggaagaac
     2401 cggggcacct tccggcgctg ctcctggccg aggcgcagcg ccttcacccc gtagaggccc
     2461 atgcccatgc ccacgtgcca acaaacgatg tcggtgaccc ggaacgggcc cttcaccagg
     2521 ggctcgagct cgtcgccctc ggccacctcc tcccaccagc ggggctcggc ccccctgcgc
     2581 cggtcgccct cggccaggac ggcctcggtg atgcgctcga tctcctcgtc ggtgtacggg
     2641 cggatctcgg tgtcgtcgta cttgcccgac gcctccgcct tctcccgctc gccccggatc
     2701 atcagccggt actgggcggc caggggcgga ccgtcctgct ggcagaagac ctccgccgtc
     2761 cactcgtgca cggcccgtcc cgcgaactcg cccgccttgt cgtggacgcc gacgagggcg
     2821 ttgcgacggg cgatccgggc cccggggtac agcggctgcc accactcgcg gtggctccct
     2881 gaatagaacg catggacccc gcgcagcggg tcccccttca tcagggcttt ggtctcgtcg
     2941 tcgtagccgg tgacctcgtt ctcgccgatg agggggtcgc cgccgaccag gtgcggcggg
     3001 gcgatcaccc cgccccagcg gctggtggcg gcgtaggccg ggtcgcacca gagcgggttg
     3061 tcgtcgccgt aggactcggc cacgtgac
//
LOCUS       DASTFV010000278         2739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1065141, whole genome shotgun sequence.
ACCESSION   DASTFV010000278 DASTFV010000000
VERSION     DASTFV010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065141"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFP02_05275"
     CDS             complement(<1..347)
                     /locus_tag="VFP02_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET9542466.1"
                     /translation="MTDAERPAGFETRAIHAGQDPDPATGAVVPPIHLATTFAQHEPG
                     KAEYEYSRSNNPTREVLEDALASLEGASHGFAFASGMAAEDAILRSLRPGDHVIVPDD
                     AYGGTWRLVDKVYG"
     gene            complement(344..2008)
                     /locus_tag="VFP02_05280"
     CDS             complement(344..2008)
                     /locus_tag="VFP02_05280"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HET9542467.1"
                     /translation="MDALVVVGADEPPEHLAARARRRIAVADPADTVTLVADLRSHRP
                     RRVGVTGPVAEAGAVAGTLHDAGLPVILYTDAPAPEAVGRGVRVLPATDPGPRMEVAD
                     RLEDLVGETPLVRLDRIGHDLDCHLLAKLELLNPGGSVKDRPALAMVDAAEREGLLQP
                     GGTIVEPTSGNTGVGLALVAARRGYHCVFVMPDKMSPEKMDLLRAYGAEVVVCPTAVA
                     PDHPDSYYSVAKRIAAERPGGYSPSQYWNDENPAAHERTTGPELWRQTAGRITHFVAG
                     IGTGGTISGVGRYLKAQNPDIRIVGADPAGSVYSGGTGRPYLVEGIGEDFWPGTYDGS
                     VVDEIVEVSDRDSFLMARAVTRTEGLLVGGSTGTAVWAALQVGRALPPDAVVVVLIPD
                     SGRGYLSKIYNEAWMADFGFLRVGEHAAGDVLGRKRADLPVLVHVHPDETVRTAIEIL
                     REYDVSQLPVVQAEPPVVLGEMVGAVRDVALMDAAFRDPGVLDRPVADVMEAKLPTVG
                     SGQVVDDVVALLEQAPAVVVIDAGHPVGVLTRSDVLDFIAGSRTRP"
     gene            complement(2047..>2739)
                     /locus_tag="VFP02_05285"
     CDS             complement(2047..>2739)
                     /locus_tag="VFP02_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542468.1"
                     /translation="LADHVLRNTDGDDRLVATTVVSSRLLSKMAAAHGVHFAETLTGF
                     KWIVRPALEHPEWRFVFGYEEALGYLVGDAVHDKDGIGAALAFAGLVADLRTQGQTVH
                     DRLDALAEEHGLHATRPLTLRFDDEDGLARIATRMTAVRQEPPTTLGGRDVEALRDLL
                     DDPDLPETDALLFSLAGGARVVMRPSGTEPKLKCYLEVVEAVAGDLAAARLRADAALD
                     ALVTDLDHRLSS"
BASE COUNT          373 a          938 c         1052 g          376 t
ORIGIN      
        1 ccgtagacct tgtcgaccag ccgccacgtg ccgccgtagg catcgtcggg gacgatcacg
       61 tggtcaccag gccgcagcga gcgcaggatg gcgtcctcgg ccgccatgcc gctggcgaag
      121 gcaaaaccgt ggctggcgcc ttccaaggac gcgagcgcgt cctccaggac ctcgcgggtc
      181 gggttgttgg agcgggagta ctcgtactcg gccttgcccg gctcgtgctg ggcgaaggtc
      241 gtggccaggt ggatgggcgg gacgacggca ccggtcgcgg gatcggggtc ctggccggcg
      301 tggatggccc tggtctcgaa acctgccggt cgctccgcgt ccgtcacggg cgggtccggg
      361 agccggcgat gaagtcgagc acgtcggaac gcgtgaggac cccgacgggg tgaccggcgt
      421 cgatgacgac gacagccggg gcctgctcga gcagggccac cacgtcgtcc acgacctggc
      481 cggacccaac cgtcggcagc tttgcctcca tgacgtccgc caccggacgg tccagcaccc
      541 cggggtcccg gaaggcggcg tccatgagag ccacatcgcg cacggcaccg accatctcgc
      601 ccaggacgac cggaggctcg gcctgcacga cgggcagctg ggagacgtcg tactcccgca
      661 ggatctcgat ggccgtccgc acggtctcgt cgggatggac gtggacgaga acgggcaggt
      721 cggcgcgctt gcggccgagg acgtcgccgg cggcgtgctc gcccacccgc aggaagccga
      781 aatcggccat ccaggcctcg ttgtagatct tggacaggta cccccgccca ctgtcgggga
      841 tgaggacgac gaccacggcg tccggcggca gcgcgcgccc gacctggagc gcggcccaca
      901 ccgccgtccc cgtggagccc cccacgagga ggccctcggt gcgggtgacg gcccgggcca
      961 tgaggaacga gtcccggtcg ctgacctcga cgatctcgtc gaccaccgag ccgtcgtagg
     1021 tgcccggcca gaagtcctca ccgatgccct cgaccaggta cgggcggccc gtgccccccg
     1081 agtagaccga acccgccggg tcggccccga cgatgcggat gtcggggttc tgggccttga
     1141 gataccggcc gacgccgctg atcgtcccgc ccgtgccgat gccggcgacg aagtgggtga
     1201 tgcgcccggc ggtctggcgc cagagctcgg gaccggtcgt gcgctcgtgg gccgcggggt
     1261 tctcgtcgtt ccagtactgg ctgggtgagt agccgccggg gcgctcggcc gcgatgcgct
     1321 tggccaccga gtagtaggag tccgggtggt cgggggcgac ggcggtcggg cagaccacga
     1381 cctcggcgcc gtaggcccgg agcaggtcca tcttctccgg gctcatcttg tcgggcatga
     1441 cgaagacgca gtggtagccc cgccgggcgg cgaccagggc caggcccacg ccggtgttgc
     1501 ccgaggtcgg ctcgacgatg gtgccccccg gctggaggag gccctcccgc tcggcggcgt
     1561 cgaccatggc cagggccggg cggtccttga cgctgccccc cgggttgagc agctccagct
     1621 tggccagcag gtggcagtcc aggtcgtggc cgatgcggtc gaggcggacc agcggggtct
     1681 ccccgaccag gtcctccagg cggtcggcca cctccatccg cggccccggg tcggtggcgg
     1741 gaaggacccg aacgccgcga ccgacggctt cgggcgcagg ggcgtcggtg tagaggatga
     1801 ccgggaggcc ggcgtcgtgg agggtcccgg ccacggcccc ggcttcggcc accggcccgg
     1861 tgacgccgac ccggcggggg cggtgagagc gcaggtcggc caccagggtc acggtgtcgg
     1921 ccggatcggc cacggcgatg cgccgccggg cccgggccgc caggtgctcg gggggttcgt
     1981 cggctccgac caccaccagc gcgtccatca gctaggtcta cccgatcgtc atcgcacccg
     2041 ttctgcttag ctcgacaggc ggtggtcgag gtcggtgacc agggcgtcga gggcggcgtc
     2101 ggcccggagg cgggcggcgg ccaggtcgcc ggcgaccgct tcgacgacct cgaggtagca
     2161 cttcagcttg ggctcggtgc cgctggggcg catgaccact cgtgcgccac ccgcgaggct
     2221 gaacaggagc gcatcggtct cggggagatc cgggtcgtcg agcaggtctc ggagtgcttc
     2281 gacgtcgcga ccgcccagcg tggtgggcgg ctcctggcgg acggcggtca tgcgggtggc
     2341 gatgcgggcc aggccgtctt cgtcgtcgaa gcggagcgtc aacgggcggg tggcgtgcag
     2401 gccgtgctcc tcggcgaggg cgtcgaggcg atcgtgcacg gtctggccct gggtcctcag
     2461 atcggcgacc aggccggcga aggccagcgc cgccccgatg ccgtccttgt cgtggacggc
     2521 gtcaccgacc aggtacccga gggcctcctc gtagccgaag acgaagcgcc actcggggtg
     2581 ttcgagggcg ggccggacga tccacttgaa gccggtcaac gtctcggcga agtggacgcc
     2641 gtgggcggcg gccatcttgc tcaggagccg ggaggacacg accgtcgtcg ccacgagccg
     2701 gtcgtcgcca tcggtgttgc gcaggacgtg gtcggccag
//
LOCUS       DASTFV010000279         3458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1072641, whole genome shotgun sequence.
ACCESSION   DASTFV010000279 DASTFV010000000
VERSION     DASTFV010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072641"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1006
                     /locus_tag="VFP02_05290"
     CDS             26..1006
                     /locus_tag="VFP02_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9542469.1"
                     /translation="MSALAKARARQALADAGLPSEGTLEPLRSVTNEVWLSDEYLIRL
                     NRRQDGRLQKELAIGGLLPPALSYPEVVAYGGEPGGDWLIVRRPRGRVLSQAWPHLAA
                     PQRNLAIQHLGHLLQTVHSLPAPAELPEIDDPPLLVSVDLESPTASLVDAINRLAPQP
                     NIDAGFMEETLGLVRDLTLSLEPFRATTMVHGDVHFENILWDGARAHTLLDWKWARPA
                     PPDVDLDVLLRFCAHPYAYVADAHRHHATPDEYEGVPWQLADAYPDLFAHHRQFDRMR
                     VFSIAYDVRELLHYPPPVPVDRLPDHHAYRRLRAVLSGDSYLDALAGAPV"
     gene            1141..2172
                     /locus_tag="VFP02_05295"
     CDS             1141..2172
                     /locus_tag="VFP02_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9542470.1"
                     /translation="MAMARARHALAKAGLDPSVELHPLSSVTNEVWLADKHIVRVNRH
                     PVPRLWREATLATMLMSAGVGCPPIVAYGGIVGADWMITEKLPGQVLSRCWPAMSLSD
                     RRMAIRQLAERLQQLHAYELREPLPEVATPQLLASSAGPGSVDRLMGGLDAAQDLEHV
                     DPVLILRTKTMVRESLATLDLVPATTLIHGDIHFENILWDGERITGLLDFEWARTAAS
                     DLELDVLLRFCAFPFLHVAEDYERVTLPEDYAPVPGWLAQDYPELFAHPHLKDRLRVY
                     SIAYDLRELLMDPPDRPVEQLSHLHPYHRLADTVDGRGHIELLELDVTDDGVTVAPLE
                     PVVVDLVSA"
     gene            complement(2092..2949)
                     /gene="lgt"
                     /locus_tag="VFP02_05300"
     CDS             complement(2092..2949)
                     /gene="lgt"
                     /locus_tag="VFP02_05300"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET9542471.1"
                     /translation="MLVLLASIPSPGSKALEIGPLSLRAYGLMIALGVLAAVEMGRRR
                     FVAKGLDPDEVSAIALWAVPAGLIGARLYHVITDWRRFEGDWWSAFAVWKGGLGVPGG
                     ILLGTLVGVWVAKRRGLPLGLAADALAPAIPLAQAIGRLGNWFNQEVFGRPTSLPWGL
                     EIDAQYRPARYATATTFHPTFLYEALWNLALMALLIWLDRRGVVRRGRLFAVYVLGYG
                     LGRFWVEALRADEASLILGIRVNLWVSALLVLGGLTWLLWPSGADEVDDDGLEGGDGD
                     AVVGDIELQ"
     gene            complement(2963..>3458)
                     /locus_tag="VFP02_05305"
     CDS             complement(2963..>3458)
                     /locus_tag="VFP02_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET9542472.1"
                     /translation="DARIPLIYEGTNGIQAMDLVGRKLPMRGGAVVGEHLDRMAATAE
                     VLTGSADEDLAAIGVRLAEGVDACREATGWLLEHGLVEPNDALAGSTPYLRLLATVTG
                     GWVLGREALTARREGADEPESFRDAKVVTARFFAENLLPSALGLVHAVSSGAAPLYAD
                     PLFV"
BASE COUNT          520 a         1340 c         1127 g          471 t
ORIGIN      
        1 cggccgctgt cctcactgtc gtctcgtgag tgccctggcc aaggcccgcg cccgtcaggc
       61 cctggccgac gccggattgc cgtcggaggg caccctcgag cccctgcgat cggtgaccaa
      121 cgaggtctgg ctgtccgacg agtacctgat ccggctcaac cgccgtcagg acggacggct
      181 ccagaaggag ctggcgatcg gtggcctcct cccgcccgcg ctcagctacc ccgaggtcgt
      241 ggcctacggc ggcgaacccg gcggtgactg gctgatcgtg cgtcgccccc gcggccgggt
      301 cctcagccaa gcctggcccc acctggccgc gccgcagcgc aacctcgcca tccagcacct
      361 gggtcacctg ctgcagacgg tccacagcct cccggccccg gccgagctgc ccgagatcga
      421 cgacccgccg ctcctggtca gcgtggacct ggagtcaccc accgccagcc tggtcgacgc
      481 catcaaccgc ctggccccgc agcccaacat cgacgcgggg ttcatggagg agacgctcgg
      541 cctggtccgg gacctgaccc tctcactgga gccgttccgg gcgacgacca tggtccacgg
      601 cgacgtccac ttcgagaaca tcctctggga cggcgcgcgg gcccacacgc tgctcgactg
      661 gaagtgggcc cggcccgctc cccccgacgt cgacctcgac gtcctgctcc gcttctgcgc
      721 ccatccctac gcctatgtgg ccgacgccca ccgccaccac gccaccccgg acgagtacga
      781 aggcgtcccc tggcagctgg cggacgcgta tccggacctc ttcgcccacc atcgccagtt
      841 cgaccggatg cgcgtcttct ccatcgccta cgacgttcgc gagctcctgc actacccccc
      901 gccggtgccc gtcgaccgcc ttccggacca ccacgcctat cgccgactgc gggccgtcct
      961 gagtggcgac agctacctcg acgcgctggc cggcgccccc gtctgaccac cctcggtgac
     1021 cctgccggtc acccgtaggt caggaggccc atgtccaccg actcatttgg cgacagagca
     1081 gtaaagaagc ggcctccaac ggccgttttc agggacatga ccggagggga gcgcggcgca
     1141 atggccatgg ccagggcccg ccatgccctg gcgaaggccg ggctcgaccc gtcggtcgag
     1201 ctgcaccccc tctccagcgt gaccaacgag gtctggctgg cggacaagca catcgtgcgg
     1261 gtcaaccgcc acccggtccc ccgcctctgg cgcgaggcca ccctggccac gatgctcatg
     1321 tccgccggcg tgggctgccc gccgatcgtg gcgtacggcg ggatcgtcgg cgccgactgg
     1381 atgatcaccg agaagttgcc cggacaggtc ctcagccggt gctggcccgc catgtcgctg
     1441 agcgatcggc gcatggccat ccgccagctg gccgagcgcc tccagcagct gcacgcctat
     1501 gagctccgtg agcccctgcc cgaggtcgcc accccgcagc tgctggcctc cagcgccggg
     1561 ccgggttcgg tggatcgcct catgggcggc ctcgacgccg cccaggacct cgagcacgtc
     1621 gaccccgtcc tcatcctgcg gacgaagacg atggtgcgcg agagcctcgc caccctcgac
     1681 ctggtgcccg ccacgaccct gatccacggc gacatccact tcgagaacat cctctgggac
     1741 ggtgagcgga tcaccggcct gctcgacttc gagtgggccc ggaccgcggc gtcggacctc
     1801 gagctcgacg tgctcctgcg cttctgcgcc ttcccgttcc tgcatgtggc cgaggactat
     1861 gagcgcgtca ccctgcccga ggactatgcc cccgtccccg gctggctggc tcaggactac
     1921 cccgagctct tcgcccatcc ccacctgaag gaccgcctcc gggtgtactc gatcgcctac
     1981 gacctgcgcg agctcctgat ggacccgccc gaccgacccg tcgagcagct gtcccacctc
     2041 cacccgtacc accgcttggc ggacacggtg gacggccggg gccacatcga gctactggag
     2101 ctcgatgtca ccgacgacgg cgtcaccgtc gcccccctcg agcccgtcgt cgtcgacctc
     2161 gtcagcgcct gagggccaga gcaaccacgt caggcccccg aggacgagca gcgcgctgac
     2221 ccagaggttg acccggatcc ccaggatcag gctggcctcg tcggcgcgca gggcctcgac
     2281 ccagaagcgg cccaggccgt agccgaggac gtagacggcg aacaggcggc ctctacggac
     2341 cacgccccgg cggtccagcc agatcaggag cgccatcaga gcgaggttcc acagcgcctc
     2401 gtagaggaac gtcgggtgga acgtcgtggc ggttgcgtag cgggccggtc ggtactgggc
     2461 atcgatctcc aggccccacg gcaggctggt cggccgtccg aagacctcct ggttgaacca
     2521 gttcccgagc cttccgatgg cctgggccag cgggatggcc ggcgctaggg cgtcggccgc
     2581 caggcccagc ggcaggcccc gccgcttggc cacccagacc ccgaccaggg tccccagcag
     2641 gatcccgccc ggcaccccga gtccgccctt ccacaccgca aaggccgacc accagtcgcc
     2701 ctcgaagcgc ctccagtcgg tgatgacgtg gtacaggcga gctccgatca gccccgcggg
     2761 gacggcccag agggcgatgg ccgagacctc gtcggggtcg aggcccttgg cgacgaaccg
     2821 gcgtcgcccc atttcgaccg ccgcgagcac acccagggcg atcatcagcc cgtaggcccg
     2881 cagcgacagg gggccgatct ccaaggcctt ggacccgggg ctcggtatcg acgcgaggag
     2941 gacgagcacg gtgccggacg gctcagacga agagcgggtc ggcgtagagc ggagcggcac
     3001 cgctggacac ggcgtggacc aggccgaggg cggagggcag caggttctcg gcgaagaagc
     3061 gggccgtcac caccttggcg tcccggaagg actcgggctc gtcggcaccc tcgcgccgag
     3121 cggtcagggc ctccctgccg aggacccagc ccccggtgac cgtggccagg agacgcaggt
     3181 acggcgtcga cccggccagg gcgtcgttgg gctcgaccaa gccgtgctcg aggagccagc
     3241 ccgtcgcctc ccggcacgcg tccaccccct cggccagccg gacgccgatc gccgcgaggt
     3301 cctcgtcggc cgagccggtc aggacctcgg cggtggccgc catgcggtcg aggtgctcgc
     3361 cgaccacggc accgccccgc atcgggagct tgcgaccgac caggtccatg gcctggatgc
     3421 cgttggtgcc ctcgtagatc agcgggatgc gggcgtcc
//
LOCUS       DASTFV010000280         4414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1076908, whole genome shotgun sequence.
ACCESSION   DASTFV010000280 DASTFV010000000
VERSION     DASTFV010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076908"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VFP02_05310"
     CDS             complement(<1..748)
                     /locus_tag="VFP02_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET9542473.1"
                     /translation="MLSLVALLGLLPVTAVRADPLPPPPGHGDIEVVGGQDADPGEDP
                     WMAALVIDPRFSPNPFAGLLCGASFISPDTLVTAAHCVRDFPIQFLDIVAGSNNLIPA
                     EVERLDVRNVRIHPLNDPFTALYDVAAIQLVHPPSFPVTPIDLVTPADAALWEPGDMA
                     RFTGWGADETGVPISRLQEAESPIVADSECLARYTRAFFRGRTMVCAGDISVGADLVS
                     PCFGDSGGPLTVLDGSRPVLIGLVLGGLRCG"
     gene            complement(825..3401)
                     /gene="otsB"
                     /locus_tag="VFP02_05315"
     CDS             complement(825..3401)
                     /gene="otsB"
                     /locus_tag="VFP02_05315"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HET9542474.1"
                     /translation="MGGPVNDELEAELEHLARTPQLLIACDYDGTIAPIVDDPMAATP
                     RPDTVVALRALAALANTHVAIISGRSLRDLAGLSRLPTEIHLVGSHGGEFDLGFGKRL
                     SPRELDLRAHIKADLTDIAARTPGSELEVKPAAVTFHYRRSDRDDVRRALQDIEAGPA
                     ALDGVRTHYNVEAVELLVVDTDKGRALESLRHRVGASAVLFFGDSPTDEHAFATLRGP
                     DVGVRVGVGDTLARHHVEDTAGVAEALARLAQLRTEWVTGAGAVAIADHSLLSDQRTA
                     AIVTPDGRITWYCAPRIDSSAVFAELVGGPAAGYFAVFPADGGEPTGQRYIEDTMVLE
                     TRWSGFTLTDYLDTSGGRPDRLAGRSDLFRVIEGRGRVRIEFAPRLDFGRHGTRLAIR
                     EGGVEVVDTADLMVLRSPGVQWELTDDGRHHTARAVVDLADEPVVLEFRYGTASTKPD
                     SRTEDERRADTRRFWQGWADELTVPDVEPDLVRRSALALKALCHGPTGAIVAAATTSL
                     PESVGGIRNWDYRYCWLRDAALTAGALARLGSYSEAMAFLDWVLGILEHRDDPERLHP
                     LYLVTGRHLPPEADIAELAGYAGSRPVRVGNAAERQVQLDVFGPIVDLIWLLATEGAP
                     LSGQHWQLVQAMVGAVEKRWLEPDHGIWEVRRSPRHHVHSKVMCWVTVDRALRVADDF
                     VDGDHRDWELLRDKIRDDVLERGWKDDVGAFTAAYDGTDVDASVLAIGLYGLVDPGDD
                     RYLRTVDTVERELREGSTVFRYRGDDGLPGAEGGFHLMTAWLVDAYLATGREADAREL
                     FGGLAAAAGQTGLLSEEIHPITGRSLGNHPQAYSHLGLIHNALNLAAYGRDGRPPADP
                     ET"
     gene            3471..4334
                     /locus_tag="VFP02_05320"
     CDS             3471..4334
                     /locus_tag="VFP02_05320"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9542475.1"
                     /translation="MRADATCYRHPDRRASVSCQRCNRPICTECMIEAPVGFHCPEDA
                     KAGQQKVITSQNLFRSAGRPIVTLTLMAVNLAVYVIGFGIEGGSERLRIDGGLLALGQ
                     KASGELIGVDAGEWYRIVTAGFLHASLLHLGFNMYLLYLLGQQLEPAMGRIRFGVTYF
                     FSLLCGSLAVLLIDPLALTVGASGAVFGLMGALAVLHYTRGVRLFDTGLGGLILINLI
                     FTFAIPGISVGGHIGGLIGGALAGWLLILLPLRSRALPSFLPTAAVLALGVAVVGASM
                     WAASQWMDPVF"
BASE COUNT          602 a         1499 c         1667 g          646 t
ORIGIN      
        1 caccacagcg cagcccgccc aagacgaggc cgatgaggac ggggcggctg ccgtcgagca
       61 cggtcagggg accgccgctg tcgccgaagc aggggctgac gaggtcggcc ccgacgctga
      121 tgtcgccggc gcacaccatc gtgcggccgc ggaagaaggc gcgggtgtag cgcgctaagc
      181 actcgctgtc ggccacgatc ggcgactcgg cctcctgcag gcggctgatc ggcacaccgg
      241 tctcgtccgc accccagccg gtgaagcggg ccatgtcgcc cggctcccag agggcggcgt
      301 cagccggcgt gaccaggtcg atgggggtca cggggaagga cggcggatgg acgagctgga
      361 tggcggcgac gtcgtacagc gcggtgaagg ggtcgttcag cggatggatc cggacgttgc
      421 ggacgtcgag gcgctcgacc tcagcgggga tcaggttgtt gcttcccgcg acgatgtcga
      481 ggaactggat ggggaagtcg cggacgcagt gggccgcggt gaccagcgtg tccggtgaga
      541 tgaacgacgc accgcagagc aggccggcga acgggttggg ggagaagcgc gggtcgatga
      601 ccagggcggc catccacggg tcctcacccg ggtcggcgtc ttggccgccg accacctcga
      661 tgtcgccgtg ccctggaggg ggcggcaacg ggtccgcccg cacggcggtg accgggagca
      721 gcccgaggag tgcgacgagg gacagcacgc acgcgcgcat ggggttcttc acgaggtgct
      781 ccgattccga ttgtccgcct ggcaaaacac tacaaccagg acgctcacgt ctccgggtcg
      841 gccggaggtc ggccgtcgcg gccgtacgcg gccaggttca gggcgttgtg gatcaggccg
      901 aggtggctgt aggcctgcgg gtggttgccg agcgagcggc ccgtgatggg gtggatctcc
      961 tccgagagca gtccggtctg gccggcggcg gcggcgaggc cgccgaacaa ctcgcgagcg
     1021 tcggcctccc ggccggtcgc caagtaggcg tcgaccagcc acgcggtcat gaggtggaac
     1081 ccgccctcgg cgccgggcag gccgtcgtcc ccgcggtagc ggaacaccgt cgagccctcg
     1141 cgcagctcgc gctcgaccgt gtcgacggtg cgcaggtaac ggtcgtcgcc cggatcaacc
     1201 agtccgtaga gcccgatggc gagtaccgag gcatcgacgt cggtgccgtc gtaggccgcg
     1261 gtgaaggccc cgacgtcgtc cttccaaccc cgctcgagga cgtcgtcgcg gatcttgtcg
     1321 cgcagcagct cccagtcccg atggtctccg tcgacgaagt cgtcggccac ccggagggcc
     1381 cggtcgacgg tgacccagca catgaccttg gagtggacat ggtggcgggg gctgcgccgc
     1441 acctcccaga tgccgtggtc cggctccagc cagcgcttct cgaccgcccc gaccatggcc
     1501 tggacgagct gccagtgctg gcccgacagc ggggcgccct cggtcgccag gagccagatg
     1561 aggtccacga tcggcccgaa gacatcgagc tggacctggc gttcggccgc gttgcccacc
     1621 cgcaccggcc ggctgccggc gtagcccgcc agctcggcga tgtcggcctc cggaggcagg
     1681 tgccgcccgg tgaccaggta cagggggtgg aggcgttcgg ggtcgtcgcg gtgctcgagg
     1741 atgccgagga cccagtcgag gaaggccatc gcctcgctgt agctccccag ccgggccagt
     1801 gcgcccgcgg tcagggccgc gtcccgcagc cagcagtagc ggtagtccca gttgcggatg
     1861 cccccgaccg actcgggcag ggaggtggtg gccgcagcca cgatcgctcc cgtgggcccg
     1921 tggcagaggg ccttgagagc caacgcgctg cgcctcacca ggtcgggctc gacgtccggg
     1981 acggtgagct cgtcggccca gccctgccag aagcgccggg tgtcggcccg gcgctcgtcc
     2041 tcggtgcgcg agtcgggctt ggtgctcgcc gtgccatagc ggaactcgag gacgacgggc
     2101 tcgtcggcca ggtcgaccac ggcccgggcg gtgtgatggc ggccgtcgtc ggtcagctcc
     2161 cactgcaccc cgggcgaccg caacaccatg aggtcggcgg tgtcgacgac ctcgacccca
     2221 ccctcccgga tggccaagcg ggtgccgtgg cgcccgaagt ccagccgggg ggcgaactcg
     2281 atccggaccc ggcctcgccc ctcgatgacc cggaagaggt cgctgcggcc cgccagacgg
     2341 tcgggacgcc cgccggaggt gtcgaggtag tcggtgaggg tgaaccccga ccagcgggtc
     2401 tcgaggacca tggtgtcctc gatgtagcgc tgaccggtcg gctcgccgcc gtcggccggg
     2461 aagacggcga agtagcccgc ggccgggccc ccgaccagct cggcgaagac ggcggaggag
     2521 tcgatgcggg gggcgcaata ccacgtgatg cgtccgtcgg gggtgacgat ggccgcggtg
     2581 cgctggtcgg acagcaggga gtggtcggcg atggccaccg ccccggcccc ggtgacccat
     2641 tcggtgcgca actgggccag gcgggccagg gcctcggcca cccccgcggt gtcctccacg
     2701 tggtgacggg ccagggtgtc gccgacgccg acccgcacgc cgacatccgg gccccgcagg
     2761 gtggcgaagg cgtgttcgtc ggtggggctg tcgccgaaga agaggacggc cgatgcgccg
     2821 acccggtggc gcagtgactc gagagcccgg cccttgtcgg tgtcgaccac gagcagctcg
     2881 acggcctcga cgttgtagtg ggtgcggacg ccgtcgaggg ccgcgggtcc ggcctcgatg
     2941 tcctggaggg cccggcgcac gtcgtcgcgg tccgagcggc ggtagtggaa cgtgacggcg
     3001 gcgggcttca cctcgagctc cgagccgggc gtacgggccg cgatgtcggt caggtcggct
     3061 ttgatgtggg cgcggaggtc cagctcacgg ggggagaggc gtttgccgaa gccgaggtcg
     3121 aactccccgc cgtgactgcc gacgaggtgg atctcggtcg gtaggcggga gaggccggcg
     3181 aggtcccgca gggaccgccc ggagatgatc gccacgtggg tgttggccag tgcggccagg
     3241 gcccggaggg cgaccaccgt gtcgggtcgc ggggtggcgg ccatggggtc gtcgacgatg
     3301 ggggcgatgg tcccgtcgta gtcgcaggcg atcaggagct ggggcgtgcg ggcgaggtgc
     3361 tcgagctccg cctccagctc gtcgttgacc ggtccgccca tgagcgatgg acgttaccgc
     3421 ccgcccccgg cgcggtcctc gatcggaggg acaccggtag cgtccccccg atgcgcgccg
     3481 acgccacctg ttaccgccat cccgatcggc gggcgtcggt gagctgccaa cgctgcaacc
     3541 gaccgatctg caccgagtgc atgatcgagg cgcccgtcgg cttccactgt cccgaggacg
     3601 ccaaggcggg ccagcagaag gtcatcacct cccagaacct gttccggtct gccggtcgcc
     3661 cgatcgtcac cctcaccctg atggccgtga acctggccgt ctacgtcatc gggttcggca
     3721 tcgagggggg gagcgagcgc ctgcgcatcg acggcgggct tctcgccctc ggtcagaagg
     3781 ccagtggtga gctgatcggg gtcgacgccg gcgagtggta ccggatcgtc accgccggct
     3841 tcctgcacgc cagcctcctc cacctcgggt tcaacatgta cctgctgtac ctgctgggcc
     3901 agcagctgga gccggcgatg gggcggatcc gattcggcgt gacctacttc ttctcgctcc
     3961 tctgtggctc gctggccgtg ctcctgatcg atccgttggc cttgaccgtc ggcgcctccg
     4021 gggcggtgtt cggcctgatg ggggcgttgg ccgtcctcca ctacacacgg ggggtccgcc
     4081 tcttcgacac cggcctcggg ggcctgatcc tgatcaacct gatcttcacc ttcgccatcc
     4141 ccggcatctc ggtcggcggc cacatcggcg ggcttatcgg cggcgccctg gccggctggc
     4201 tcctgatcct cctgccgctc cgctcccggg ccctgccctc gttcctcccc accgccgcgg
     4261 tcctggccct gggggtggcc gtggtcggcg cctcgatgtg ggccgcgtcc caatggatgg
     4321 atcctgtttt ctagggctgt tccacgaggt ccttgaggcg ggtgaggttc cggcgccaga
     4381 cggcgcggag gacgggggcg ccggcggcgc cgcc
//
LOCUS       DASTFV010000281         2485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1077378, whole genome shotgun sequence.
ACCESSION   DASTFV010000281 DASTFV010000000
VERSION     DASTFV010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077378"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VFP02_05325"
     CDS             <1..565
                     /locus_tag="VFP02_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542476.1"
                     /translation="GFITAVGVHRDVTGTAQARTLAVAKGIGGLRQGAIELTVRQEAV
                     LDLAVEQVLSPALTHVSNSFVGVMLEQGIPLEAVITELVLSGEVERTYRLLREEGFAR
                     QMAYHSPTSQYGQLSRRGRYDHLDVGTTMRELVDDITSGRFADEWDAEQVTGHARLAA
                     LTAAHAGPEVQAFEDEMRTRLGEQAAG"
     gene            695..1930
                     /locus_tag="VFP02_05330"
     CDS             695..1930
                     /locus_tag="VFP02_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HET9542477.1"
                     /translation="MDDKPRNLRSMLTEAKDASELMLDLAYASVYFGDPDMAEEVDEL
                     EDQMNDLVHEMQAVCILAARKPSEADEMASVLQVISAIERIANAAANIARIVTHRLGI
                     PRELVADLSEAEEVAHRVLIREGSHMAHRPLAALELPVATGMRIMAIRRGREWIIDEI
                     RGDQVILPGDVLFCEGSPAGITRLRELAGASAWAPAMVPDGAVLTDLDRAVDTLVEMK
                     NISEAAVGLAYSALVLGDRSLALEVRHLEDRLDEMKDRLEVWVLRAAAPDIDPSPLRG
                     LLALSQAAEDIGDQAQQMVWLIEHGEEIHPILGIALGESDEVVLRIPVAQGSAADGAK
                     LSELELEIEPGFQILAIQRDGRYRYQPRRYVQLEAGDELIVSGPDEGRALMAERLGWR
                     FTLDEETGEHELLPLDRVS"
     gene            complement(1940..>2485)
                     /locus_tag="VFP02_05335"
     CDS             complement(1940..>2485)
                     /locus_tag="VFP02_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HET9542478.1"
                     /translation="AKVVAVSDVHGGVHHPDGLDIIDLCRTVLNGGRLADAEGVTVID
                     NEELLEIDCDVLIPAALGQVLTGANAREVKAGIVVEAANYPVTPDGDAALHEMGVTII
                     PDILANAGGVVGSYFEWTMNIQQFTWDEAQFNQRLAARLLKAYAATREMADYLECTLR
                     SGAYAIGVRRVAETVELRGYI"
BASE COUNT          374 a          874 c          889 g          348 t
ORIGIN      
        1 cggcttcatc accgccgtcg gcgtgcaccg ggacgtcacg ggcacggccc aggcccgcac
       61 gctggccgtg gccaagggca tcggtggcct gcgccagggc gcgatcgagc tcaccgtccg
      121 gcaggaggcc gtcctcgacc tggccgtcga gcaggtcctc tcaccggcgc tgacccatgt
      181 gtccaacagc ttcgtcggcg tgatgctgga gcagggcatt cccctggagg ccgtcatcac
      241 cgagctggtc ctgtcgggcg aggtcgagcg cacctatcgc ctcctgcggg aggaggggtt
      301 cgcccggcag atggcgtacc actcgccgac cagccagtac ggccagctgt cccgtcgggg
      361 tcgctacgac cacctcgacg tcgggaccac gatgcgggag ctggtcgacg acatcacgtc
      421 cgggcgcttc gccgacgagt gggacgccga gcaggtcacc ggccacgccc ggctggcggc
      481 cctgaccgcg gcccacgccg gcccggaggt ccaggccttc gaagacgaga tgcggacccg
      541 gctgggggag caggccgcgg gctgacccgc gggcggggta ctccggctga aagaccacct
      601 accctgaggc aatgacgctg cgcagcctcg cggtgcagct ccgacgaccg gacccgatcg
      661 gcggagccgg ccggacggag tacggcggaa ggtgatggac gacaagcccc ggaacctccg
      721 ctcgatgctc acggaggcca aggacgcgtc cgagctgatg ctcgacctgg cctacgcgtc
      781 ggtctacttc ggtgacccgg acatggccga ggaggtcgac gagctcgagg accagatgaa
      841 cgacctcgtc cacgagatgc aggccgtgtg catcctggcg gcccgcaagc cgagcgaggc
      901 cgacgagatg gcctcggtgt tgcaggtcat cagcgccatc gaacgcatcg ccaacgccgc
      961 ggccaacatc gcccgcatcg tcacccaccg cctgggcatc ccccgggagc tggtggccga
     1021 cctgtccgag gccgaagagg tggcccaccg cgtcctcatc cgggaggggt cgcacatggc
     1081 ccaccgaccg ctggccgccc tcgagctgcc ggtggccacg ggcatgcgca tcatggccat
     1141 ccgccggggt cgggaatgga tcatcgacga gatccgcggc gaccaggtca tcctccccgg
     1201 cgacgtgctc ttctgcgagg gctcgccggc cgggatcacc cgtctccggg agctggccgg
     1261 ggcgtcggcc tgggcgccgg cgatggtgcc cgacggcgcc gtgctcaccg acctcgaccg
     1321 ggccgtggac acgctcgtgg agatgaagaa catctccgag gccgccgtcg gcctggcgta
     1381 ctcggccctc gtgctcggcg accgcagctt ggccctggag gtccgccacc tggaggaccg
     1441 gctggacgag atgaaggacc gcctcgaggt gtgggtcctg cgggccgccg cccccgacat
     1501 cgacccgtca cccctacggg gcctgctggc cttgtcccag gccgccgagg acatcggtga
     1561 ccaggcccag cagatggtct ggctcatcga gcacggcgag gagatccacc cgatcctcgg
     1621 catcgccctc ggcgagagcg acgaggtggt gctccgcatc cccgtggccc agggctcggc
     1681 cgccgacggg gccaagctca gcgagctgga gctggagatc gagcccggct tccagatcct
     1741 ggccatccag cgggacggcc gctaccgcta ccaaccccgc cggtacgtcc aactcgaggc
     1801 gggtgacgag ctcatcgtca gcggccccga cgagggtcgc gccctcatgg ccgagcgcct
     1861 cggttggcgg ttcaccctcg acgaggagac cggcgagcac gagctcctcc ccctcgaccg
     1921 ggtcagctga cctcgggggt cagatgtagc cgcggagctc gacggtttcg gcgacccggc
     1981 ggacgccgat ggcgtaggcg cccgagcgca gggtgcactc gaggtagtcg gccatctccc
     2041 gggtggcggc gtacgccttc aggaggcggg cggcgagacg ctggttgaac tgggcctcgt
     2101 cccacgtgaa ctgctggatg ttcatcgtcc actcgaagta gctgcccacg acgccaccgg
     2161 cgttggccag gatgtccggg atgatggtga cgcccatctc gtgcagggcg gcgtcgccgt
     2221 cgggggtgac ggggtagttg gccgcctcga cgacgatgcc ggctttgacc tcgcgggcgt
     2281 tggcgccggt caggacctgg ccgagggcgg cggggatgag cacgtcgcag tcgatctcca
     2341 gcagctcctc gttgtcgatg accgtcacgc cctcggcgtc ggccaatcgg ccgccgttca
     2401 ggacggtccg gcacaggtcg atgatgtcca agccgtcggg gtggtggacg ccaccgtgca
     2461 cgtcggagac ggcgacgacc ttggc
//
LOCUS       DASTFV010000282         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1083075, whole genome shotgun sequence.
ACCESSION   DASTFV010000282 DASTFV010000000
VERSION     DASTFV010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083075"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VFP02_05340"
     CDS             complement(<1..802)
                     /locus_tag="VFP02_05340"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9542479.1"
                     /translation="MRVSEERETVTITVNGEPVVAEKGELVIAAAERAGTYIPHFCYH
                     PRMASVGMCRMCIVDVDTGRGPALQPACMIEVAPDMVVDTHSEATKKAQDGVLEFLLI
                     NHPLDCPVCDKGGECPLQDQTIAYGPGESRFVEEKRHYAKPIPVNDVVLLDRERCILC
                     DRCTRFAKDVAGDPLISFIGRGAATEVNTFPDHPFSSYFSGNTVQICPVGALTAGPFR
                     FRARPWDLANAESTCTTCSVGCRVAIQSSRDEVLRYSGVDGDPVNWGWL"
     gene            complement(789..2147)
                     /gene="nuoF"
                     /locus_tag="VFP02_05345"
     CDS             complement(789..2147)
                     /gene="nuoF"
                     /locus_tag="VFP02_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HET9542480.1"
                     /translation="MAPLASTDRIVSRRLEQSDAHTIAGYEGTGGYDGLKAALGRPPQ
                     EIVDEVKTASLLGRGGAGFPCGVKWGFCPPGVWPRYVVINGDESEPGTYKDRALMERD
                     PHQVVEGALICAYAVGAAQVFLYVRGEMALAQERLAQALNDAYAKGYVGENIAGTDFS
                     CDVVLHWGAGAYIVGEETGLIESLEGNRGMPRLKPPFFPAAKGLYLQPTIVNNIETVC
                     NLPWIVLNGGDAFAEKGAETSRGTRLFAVSGHVQRPGIYEVEFGVTTYRDLLFDPEYG
                     GGIREGHALKCFIPGGASAPWFFDEHLDLPLETGAVGKAGSMMGSGAVVVMDDTTDAV
                     RAAWRVVRFFANESCGKCTPCREGTNWLAKIMRRILDGYGRPSDLDLLRDVCDNISPG
                     LAWPPRMTTICPLGPSAVSPILSTIDRFRDEYDAYIAAAVPAPTTVSIPVSIRAKEDA
                     GV"
BASE COUNT          319 a          747 c          847 g          334 t
ORIGIN      
        1 agagccagcc ccagttgacc gggtcgccgt cgaccccgct gtagcgcagg acctcgtccc
       61 gggaggactg gatggccacc cggcagccga ccgagcaggt cgtgcaggtg gactcggcgt
      121 tggccaggtc ccaggggcgg gctcggaagc ggaagggccc ggccgtgagg gcgcccaccg
      181 ggcagatctg gaccgtgttg ccgctgaagt aggacgagaa cgggtggtcg gggaaggtgt
      241 tcacctccgt cgccgcgccc cggccgatga aggagatcag ggggtcgccg gcgacgtcct
      301 tggcgaagcg ggtgcagcgg tcacagagga tgcagcgctc ccggtcgagg aggacgacgt
      361 cgttgaccgg gatgggcttg gcgtagtgcc gcttctcctc gacgaagcgg ctctcgcccg
      421 ggccgtacgc gatggtctgg tcctgcaggg ggcactcgcc acccttgtca cagaccgggc
      481 agtccagcgg gtggttgatg agcaggaact cgaggacgcc gtcctgggcc ttcttggtgg
      541 cttcgctgtg ggtgtccacc accatgtcgg gagccacctc gatcatgcag gcgggctgca
      601 gggccggacc ccgacccgtg tcgacgtcca cgatgcacat gcggcacatg ccgaccgacg
      661 ccatgcgcgg gtggtagcag aagtggggga tgtaggtccc ggcccgctcg gccgcggcga
      721 tgaccagttc gcccttctcg gccaccacgg gctcgccgtt gacggtgatg gtcaccgtct
      781 cccgctcctc agacacccgc atcctccttg gctcggatgc tgaccgggat ggacaccgtg
      841 gtcggcgcgg gcacggccgc ggcgatgtag gcgtcgtact cgtcccggaa gcggtcgatg
      901 gtggacagga tgggcgacac ggcggacggt cccagcgggc agatggtggt catccggggc
      961 ggccaggcca gcccggggct gatgttgtcg cagacgtccc gcagcaggtc caggtccgac
     1021 gggcggccgt agccgtccag gatccggcgc atgatcttgg ccagccagtt ggtgccctca
     1081 cgacaggggg tgcacttgcc gcacgactcg ttggcgaaga agcgcacgac ccgccaggcg
     1141 gcccgcacgg cgtcggtggt gtcgtccatg accacgaccg cgcccgagcc catcatggag
     1201 ccggcctttc cgacggcccc ggtctccagc ggcaggtcga ggtgctcgtc gaagaaccac
     1261 ggggccgacg cgccgccggg gatgaagcac ttcagggcgt gaccctcgcg gatgccgccg
     1321 ccgtactccg ggtcgaagag caggtcccgg taggtggtga cgccgaactc gacctcgtag
     1381 atgccggggc gctggacgtg gccggagacg gcgaagaggc gggtgccccg gctggtctcg
     1441 gcccccttct cggcgaaggc gtcgccgccg ttgagcacga tccagggcag gttgcagacg
     1501 gtctcgatgt tgttgacgat cgtgggctgg aggtagaggc ccttggccgc cgggaagaac
     1561 gggggcttga gccggggcat gccccggttg ccctcgaggc tctcgatgag gccggtctcc
     1621 tcacccacga tgtaggcgcc ggcgccccag tgcaggacga cgtcgcagga gaagtcggtg
     1681 ccggcgatgt tctcgccgac gtagcccttg gcgtaggcgt cgttcagggc ctgggccagt
     1741 cgctcctggg ccagggccat ctcgccccgc acgtagagga agacctgggc ggcgccgacg
     1801 gcgtaggcgc agatgagggc gccctccacc acctgatggg ggtcacgctc catcagggcc
     1861 cggtccttgt aggtgccggg ctcgctctcg tcgccgttga tcacgacata gcggggccac
     1921 acgcccgggg ggcagaagcc ccacttgacc ccgcacggga acccggcgcc accgcggccc
     1981 agcaggctgg cggtcttgac ctcgtcgacg atctcctggg gaggtcggcc cagcgccgcc
     2041 ttcaggccgt cgtacccacc cgtgccctcg tacccggcga tggtgtgggc gtcggactgc
     2101 tccaggcgcc ggctgacgat gcggtcggtg ctggccagag gcgccatgtc aggccccctc
     2161 ggccggggcc atccacgccg gcgcctggcc cccgtcgggg agggcggggc cggcggcgcg
     2221 ctcgggcggg atgtgctggc ggatgcg
//
LOCUS       DASTFV010000283         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1083525, whole genome shotgun sequence.
ACCESSION   DASTFV010000283 DASTFV010000000
VERSION     DASTFV010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083525"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFP02_05350"
     CDS             <1..573
                     /locus_tag="VFP02_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542481.1"
                     /translation="AGLRAAIGLGKAGADVALKVLDQSPVGDAAGSLADRLEPAAERG
                     RRQRQATAEEVEAVMGRVVDDVVEVLDLNGIIQQVDVNAIVERVDVNEVVGRVDIDDI
                     ISRVDVDGIVRRTEVGTLIVQSTGGIVTQVLDFVRSLGVSLDTVAERVVDRILHHGRP
                     RPVAPVLLAGSAADVVESGPSLPAPSERSA"
     gene            570..1079
                     /locus_tag="VFP02_05355"
     CDS             570..1079
                     /locus_tag="VFP02_05355"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET9542482.1"
                     /translation="MRAEPPTGSLAGHYAGPVTRLSAYAVDAFVIVATYGLMLSLITF
                     VWQLVSRRDLELPASDTLVWLIGLGIWTFLYFAASWGLVAKTVGMAVVGIRVVGRDGG
                     ELTARRGLVRAMAFPLSFLLFGLGFVGIVVGRERRALHDVIAHTTVVYDWDARSARLR
                     LLTRRAAEA"
     gene            complement(1086..1574)
                     /locus_tag="VFP02_05360"
     CDS             complement(1086..1574)
                     /locus_tag="VFP02_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542483.1"
                     /translation="MIRVAMALGALALVLAGCGDDEDAAPPSTTAVTVTVDEPLPMGA
                     SYEDRSGNVTLTVHGVRITGGGLVLADAEACLSASALPSLPIQASAWQLQLRGREQTV
                     PRTTVDQPTQATSPPWPDDVELEAGECFEGKVAFQVPDGGRASGIVFTQLSTAVAWTV
                     RS"
     gene            1617..>2508
                     /locus_tag="VFP02_05365"
     CDS             1617..>2508
                     /locus_tag="VFP02_05365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03564 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET9542484.1"
                     /translation="MRIGIHGGSVMIMGAAIEEVAAHAAEAEADGFASYWVPQSGGTD
                     ALTVIAVLGAATSTIEFATAVIPTWGRHPHVLAGQALTVQAATGNRLALGIGVAHKPS
                     VEDRYHIPFERPVRHLREYLDVLQPLLAERQADVVGEIWSFTGDLVAPPAEPPPVLVA
                     ALGPQFLRLAGKRADGTVLWLVGPTTVADHIVPTIADAADEAGRAAPRVVASVPVSVT
                     DRPDEVRSFISEILAGYDTLPSYRAMLDREGAVRAGDVAVIGNEEEVRDGLARFADAG
                     ATDFAAVEIGLDSEGFARTRA"
BASE COUNT          311 a          913 c          914 g          370 t
ORIGIN      
        1 gccggcttgc gagccgccat cggcctcggc aaggccgggg cggacgtcgc cctgaaggtg
       61 ctggaccaga gcccggtggg tgatgccgcc ggctccctgg ccgaccgcct ggagcccgcg
      121 gccgagcggg gtcgccgcca gcgccaggcc accgccgagg aggtcgaggc cgtcatgggc
      181 cgggtcgtgg acgacgtcgt ggaagtgctc gacctcaacg ggatcatcca gcaggttgac
      241 gtgaacgcca tcgtcgagcg ggtcgacgtc aacgaggtcg tgggtcgggt cgacatcgac
      301 gacatcatct cgagggtcga cgtggacggc atcgtgcgcc gcaccgaggt cggcaccctc
      361 atcgtgcagt ccaccggtgg catcgtgacc caggtcctcg acttcgtccg cagcctcggg
      421 gtctccctgg acacggtggc cgagcgggtc gtcgaccgga tcctgcacca cggccgtccc
      481 cggccggtgg cccccgtcct cctggccggc tcggcggctg acgtcgtcga atcggggccg
      541 tcgctcccgg cgccgtctga gcgttccgca tgagagccga gcccccgacc ggctcgctgg
      601 ccgggcacta cgccgggccg gtcacccggt tgagcgccta cgccgtcgat gccttcgtca
      661 tcgtggccac ctacgggctg atgctcagcc tcatcacctt cgtctggcag ctggtgagca
      721 ggagggacct cgagctgccg gccagtgaca ccctcgtctg gctgatcggg ctcgggatct
      781 ggaccttcct ctacttcgcc gcgagttggg ggctggtggc gaagaccgtc ggaatggccg
      841 tcgtcggcat tcgcgtggtc gggcgggacg gcggcgagct gacggcccgc cggggcctcg
      901 tgcgggccat ggcgttcccg ctgagcttcc tcctgttcgg cctcggcttc gtcggcatcg
      961 tcgtcgggcg cgaacgccgg gcgctgcacg acgtgatcgc tcacaccacg gtggtctacg
     1021 actgggatgc ccggtccgcc cgcctacgcc tgctcacccg gcgggccgcg gaggcctgag
     1081 cccgctcagc tccggacggt ccacgccacg gccgtgctga gctgggtgaa cacgatcccg
     1141 ctcgcccggc cgccgtcggg cacctggaag gccaccttgc cctcgaagca ctcaccggcc
     1201 tccagttcga cgtcgtcggg ccacggcggg ctcgtggcct gcgtcggctg gtcgacggtg
     1261 gtccggggga cggtctgctc ccggcctcgg agttggagct gccaggccga cgcctggatg
     1321 ggaaggctcg gcagggcgct ggcgctgagg caggcctcgg cgtcggccag cacgaggccg
     1381 ccgccggtga tgcgcacgcc gtgcacggtc agcgtgacgt ttccggagcg gtcctcgtag
     1441 ctggcaccca ttggcagtgg ctcgtccacg gtgacggtga ccgcggtcgt cgacggcggg
     1501 gcggcgtcct catcgtcccc acacccggcg aggaccaggg cgagcgctcc cagggccatg
     1561 gcaacgcgga tcatcggggc cgaacctacc tgtgggtgga gggctacggt ccgcacatgc
     1621 gcatcgggat ccacggcggt tccgtcatga tcatgggggc cgccatcgag gaggtcgccg
     1681 cccacgcggc cgaggccgag gccgacgggt ttgccagcta ctgggtcccc cagagcgggg
     1741 gaaccgacgc cctgaccgtg atcgcggtcc tcggggcggc cacctccacc atcgagttcg
     1801 ccaccgcggt gatcccgacc tggggtcgac acccccatgt cctggccggt caggccctca
     1861 ccgtgcaggc ggccacgggc aaccggctcg ccctcggcat cggcgtggcc cacaagccgt
     1921 cggtcgagga ccgctaccac atccccttcg agcgcccggt gcgccacctg cgggagtacc
     1981 tcgacgtgct ccagcccctc ttggccgagc gccaggccga cgtcgtcggt gagatctggt
     2041 ccttcaccgg cgacctggtg gccccgcccg ccgagccgcc gccggtgctg gtcgccgccc
     2101 tcgggccgca gttcctccgc ctggccggca agcgggccga cggcaccgtc ctctggctgg
     2161 tggggccgac gacggtggcc gaccacatcg tgccgaccat cgccgacgcg gccgacgagg
     2221 ccgggcgcgc cgcgccccgg gtggtggcca gcgtccccgt ctcggtgacc gaccgccccg
     2281 acgaggtgcg gtccttcatc tccgagatcc tggccgggta cgacacgctg ccgagctatc
     2341 gggccatgct cgaccgtgag ggtgcggtcc gggccgggga cgtggccgtc atcggcaacg
     2401 aggaggaggt ccgggacggg ctggcccgct tcgccgacgc gggagcgacg gacttcgccg
     2461 cggtggagat cggcctcgac agcgaggggt tcgcccggac ccgggccc
//
LOCUS       DASTFV010000284         7216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1086918, whole genome shotgun sequence.
ACCESSION   DASTFV010000284 DASTFV010000000
VERSION     DASTFV010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086918"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1678
                     /locus_tag="VFP02_05370"
     CDS             <1..1678
                     /locus_tag="VFP02_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET9542485.1"
                     /translation="SRLRTDVFRPATGGPHPVLVHRYPYSPRDGFGAMFGRMIATQGY
                     AVVVQSCRGRFGSEGDFSPIHPDVEDGYDTVEWAAAQPWSNGKVGMYGASYGGMTQWT
                     AAMGGPPHLVCLAPFTSTWDATVGGWFSAPGVLTLGLAVLWSAQMTAFEAERRGVEPP
                     LPAFAELARLQDGGALGDPDGMAQFIQLTKDGAETLFGRRPLRDIAELAELAPWFRDW
                     CDHEDPRDPYWRKTSAADHLDEIDLPILHLTGWYDYFTKGTLDGYTKMARYGRTEETR
                     RSQRLIAGPWNHNTMQVRPDADPGVSLFFDFSPGTPVMRFFAHHLKGELPDYGNEPPV
                     RIYVMGENVWREEQEWPLARTKWTPWHLRSGDGERALSGELPADEPPDSYVYDPADPV
                     PGSMAIGPTYDDPVDLEAVAARADVLVYATDPLVEDTEITGPVTFELWASSSAPSTDF
                     TAKLIEVFPDGRAVPLCQGVVRTSVGVAQAPLPGAAYRYEIDLGVTSVLVKAGHRVRL
                     DVSSSEYPTYEPNPNTGGRITTDAETVPATQHVFHDVLHPSRLILPIIPR"
     gene            1699..3054
                     /locus_tag="VFP02_05375"
     CDS             1699..3054
                     /locus_tag="VFP02_05375"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HET9542486.1"
                     /translation="MAVTPAELRRLADDGSTVFAPYASETPPFSEHDYVQEEWVATGV
                     EDGHAYATTVLVRRPRDRARFSGTVVVAPLHFHGIEPIWIYTAAYLLRSGHAWVEVTA
                     QKTTLDLHVKPSNPDRYADLDIDGPDSGDLDLALHLDDREANNAFWSDLVRRNRATSA
                     ILAQVGAALRGSAGPFAGLEVRSVLLAGHSQTGSVTSYFIEDAHDQQRREDGSAVYDG
                     FFPTGFPYKPFRDIGVPVVQVMSDGDVSLPDYSFRPGYEGRAYRREDSDEPGDRYRLY
                     ELAAVPHMGTRYAPFDDIALWQAQHGEDAAGDGAVFGSRMNSLPHFELFSVCLDHLVR
                     WVEEGTVPPRAERLEIGPDGLFTKDEHGNTRAGVRCVQLDVPHATFHANLLRADGTPT
                     WLTVGCEEPFAAEQLRALYGDKAGYLARFNHRLDELVTEGWLLPDDAAEMMREAETLD
                     F"
     gene            3054..5237
                     /locus_tag="VFP02_05380"
     CDS             3054..5237
                     /locus_tag="VFP02_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET9542487.1"
                     /translation="MDVDDLLGQMTLEEKAAQLTGVWYSDLLVEGQLDDERMTQHLSH
                     GIGQITRIAGTGFDPVPAAEAIDRIQRFLEDRTRLGIPALAHEEALSGLMAPGATNFP
                     QAIGLASTWDPVLVEEVAVAIGRQVRAVGARLALSPVLDIARDPRWGRLEETYGEDPE
                     LVSRMGVAYVRGVQSQGVDCCGKHFLGHASTLGGLNHGQVVLGPRRLRDVEAVPFRAA
                     IHEAGLATVMNAYNDLDGLPIVGSPEIMTDLLRGELGFEGCVVADYYSIDDLDGLHHI
                     VADREEAARMALSAGIDVELPSAVYYATLADQVRSGRLDEAVVDQACRRVLEEKLALG
                     LFDERYVGTEAADGIETVEDVALARRASARSIVLLTNDGTLPLAPGARVAVLGPSATD
                     TRRLYGDYSYPGRSIHLGVDQVVADVVAPNDLRRIARRVPAPRQALAERYEVVDDLDG
                     ADVAVVIVGGQSGMSEEDTSGEFRDASDLRLPPEQLDLIATTAASGVPTVVVVVGGRA
                     HSLAEVVPHAAALVLAWLPGDEGGRGLVDVLSGDVDAGGRLPVSLLHRVGQVGGYPGH
                     HHGGGRSLMYGDYVDGPVAPLFPFGHGLSYTSWSYDDVAVAAGSTTGQTVIDVALTNT
                     GDREGEEVIQVFARDELASVGVPARRLVAFQRVGAEAGETVRVRFTIPATSLGFHGVD
                     LRFRVEPGDVTFLVGPTATTVTVTGDVEHPDPNAPRPFTVDVTSA"
     gene            complement(5247..6875)
                     /locus_tag="VFP02_05385"
     CDS             complement(5247..6875)
                     /locus_tag="VFP02_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9542488.1"
                     /translation="MTASIDELVAEYGGEDLGLAHVLCDRHPADAPAFTVVEADLRAV
                     TLTYGQLRESSERLAAAFAELGIGPGDRIATLMGKSVDYVTTVVAIWRLGAVHVPLFT
                     AFAPPAIAFRLEASHTKAVVCDDGQRHKLDPGTDIREDADWTVVVAGPGAERREGDLL
                     LQDLVDAQEAGFPAAALGRDAPMIHIFTSGTTGTPKGVVVPAFALAAIQAYMEYGFDV
                     REDDVFWCGADPGWAYGLYYAIVGPLALGACALLLDAPFSPAVAWAVLADHRVTNFAA
                     APTVYRSLRAASDDVPELALRRASSAGEPLTPDVNEWATGALGVTVHDHYGQTEAGML
                     VNNHHHPDVAQPLKPGSMGRPMPGWSIHVLETDRDEPAEPGVVGRIAADMTASPLAWF
                     HHYEDAPEATAAKFSADRRWYHTGDTAYVDEDGDIHFTARDDDIIIMAGYRIGPFEVE
                     SVLNGHPSVLESAAIAVPDEIRGEVLEAHVVLNAGHTGSPELAAELQQLVKTGLAAHA
                     YPRNVIFIDEMPKTPSGKVQRFVLRDQRRRELAS"
     gene            6980..>7216
                     /locus_tag="VFP02_05390"
     CDS             6980..>7216
                     /locus_tag="VFP02_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542489.1"
                     /translation="MSVTTQQITPEIGLEVTGLAGHELVDPDRAAECQAALDAHGVVV
                     YREAGIGDADLLAFTEMLGTVVIPKMGGLEEHPGV"
BASE COUNT         1024 a         2597 c         2523 g         1072 t
ORIGIN      
        1 cagtcggctc cgcaccgacg tgttccgccc agccacgggc ggcccccacc cggtcctcgt
       61 ccatcgctac ccgtacagcc cgagggacgg cttcggggcc atgttcggac ggatgatcgc
      121 cacccagggc tacgcggtcg tcgtccagag ctgccgcggc cgcttcggct ccgaaggcga
      181 cttctctccc atccaccctg acgtcgagga cggttacgac accgtcgagt gggccgccgc
      241 ccagccgtgg tcgaacggca aggtcgggat gtatggggcg tcctatggcg gcatgacgca
      301 gtggaccgcc gccatgggcg gtccgccgca cctcgtctgc ctcgcgccct tcacgtccac
      361 ctgggacgcg accgtcggcg gatggttctc cgcccccggc gtcctgacct tgggcctggc
      421 cgtcctctgg agcgcccaga tgaccgcctt cgaggcggag cgacgagggg tggagccacc
      481 cctgcccgcg ttcgccgaac tggcgaggct gcaagacggt ggtgctctcg gcgatcccga
      541 cggcatggcg cagttcatcc agctgacgaa ggacggagcg gagaccttgt tcgggcgccg
      601 cccccttcgg gacatcgccg agctggcgga gctggcgccg tggttccgtg actggtgcga
      661 ccacgaggac ccgcgggacc cctattggcg caagaccagc gcggccgacc acctcgacga
      721 gatcgacctg ccgatcctgc acctcaccgg ttggtacgac tacttcacca agggcaccct
      781 cgacggctac acgaagatgg cgcgctacgg acggaccgag gagacgcggc gtagccagcg
      841 cctcatcgcc ggcccgtgga accacaacac gatgcaggtc cgtcccgacg ccgatccggg
      901 cgtttcgctg ttcttcgact tcagtccggg gacgcccgtc atgcggttct tcgcccacca
      961 cctcaagggc gagctccccg attacggcaa cgagcccccc gtccgcatct acgtgatggg
     1021 ggagaacgtt tggcgggagg agcaagagtg gccgctggcc cggacgaagt ggacgccctg
     1081 gcacctgagg agcggcgacg gtgaacgcgc cctgtcgggt gaactcccag ccgacgaacc
     1141 gccggacagc tacgtctacg acccggccga tccggtcccg ggctcgatgg cgatcgggcc
     1201 gacctatgac gaccccgtcg acctcgaggc cgtggccgcc cgggctgatg tcctcgtcta
     1261 cgcgaccgac cccctggtcg aggacaccga gatcaccggc cccgtgacct tcgagctctg
     1321 ggcctcgtcg tcggcgccga gcaccgactt cacggccaag ctcatcgagg tcttccccga
     1381 cggccgcgcc gtccccctct gtcagggcgt ggtgcgcacc agcgtcggtg ttgctcaggc
     1441 tccgctaccg ggcgcggcgt accggtacga gatcgatctg ggggtgacga gcgtcctcgt
     1501 gaaggcgggc caccgtgttc gtctcgacgt gtcctccagc gagtacccga cctacgagcc
     1561 gaacccgaac acgggcggcc gcatcacgac cgatgcggag acggtgccgg cgacccagca
     1621 cgtcttccac gacgtcctcc acccctcccg cttgatcctc ccgatcatcc cccgctgacc
     1681 cgaccagaag gacagaccat ggccgtgacc cctgccgagc tccgccgcct cgcggacgac
     1741 ggctcgaccg tcttcgcccc gtacgcgtcg gagacccctc cgttctcgga acacgactac
     1801 gtgcaagagg agtgggtcgc cactggcgtc gaggacggcc acgcctatgc cacgacggtg
     1861 ctggtgcggc gaccccgcga ccgcgcccgg ttcagcggca cggtggtcgt cgcgcccctg
     1921 catttccatg gcatcgagcc gatctggatc tacactgccg cgtacctcct gcggtccggg
     1981 cacgcctggg tcgaggtcac tgcgcagaag acgaccctcg acctccacgt caagccgtcc
     2041 aaccccgacc gctacgccga cctggacatc gatggcccgg acagcgggga tctcgacctg
     2101 gccctgcacc tcgacgaccg cgaggccaac aacgcgttct ggtccgacct ggtgcgccgg
     2161 aaccgagcca ccagcgcgat cctcgctcag gtcggcgctg ccctacgagg gtcggcgggc
     2221 ccgttcgccg ggctcgaggt ccgcagcgtc ctcctcgccg gccactccca gaccggcagc
     2281 gtgaccagct acttcatcga ggacgcgcac gatcagcagc gccgggagga cgggtcggcg
     2341 gtctacgacg gcttcttccc cacggggttc ccctacaagc cgttccgcga catcggcgtt
     2401 cccgtcgtgc aggtcatgag cgacggcgac gtgtcccttc ccgactactc cttccgcccc
     2461 ggctacgaag gtcgtgccta ccgccgtgag gacagcgacg aacccggcga ccggtaccgg
     2521 ctgtacgagc tcgccgcggt tccgcacatg ggcacccggt acgcgccctt cgacgacatc
     2581 gcgctgtggc aggcccagca cggtgaggat gcggcggggg acggtgccgt cttcggctcg
     2641 cggatgaaca gcctgcccca cttcgagctc ttctcggtct gcctcgacca cctggtgcgg
     2701 tgggtggagg aggggaccgt cccaccccgg gccgagcggc tcgagatcgg ccccgacggg
     2761 ctgttcacca aggacgagca cgggaacacc cgcgccggcg tgcgctgcgt gcagcttgac
     2821 gtgccgcacg cgaccttcca cgccaacctc ctcagggccg acggcacccc gacctggctc
     2881 accgtcggct gcgaggagcc gttcgccgcc gagcagctgc gggcgctgta cggcgacaag
     2941 gcggggtacc tcgcgcgctt caaccaccgg ctggacgagc tcgtcaccga gggctggctt
     3001 cttccggacg acgccgcgga gatgatgcga gaggccgaga cgctcgactt ctgatggacg
     3061 tcgacgacct cctcgggcag atgacgctcg aggagaaggc cgcccagttg acgggcgtct
     3121 ggtactccga cctgctcgtg gagggccagc tcgacgacga gcggatgacg cagcacctgt
     3181 cgcacggcat cggccagatc acgcgcatcg cgggcacggg gttcgatccg gttcccgcgg
     3241 ccgaggccat cgaccggatc cagcgcttcc tcgaggaccg gacccgactg ggcatcccgg
     3301 cactcgccca cgaggaggcc ctctcggggc tgatggcccc cggtgccacg aacttccccc
     3361 aggccatcgg cctcgcgtcg acctgggacc cggtcctggt ggaggaggtc gcggtcgcca
     3421 tcgggcggca ggtccgcgcc gtcggtgccc gcctcgcgct ctcgcccgtc ctcgacatcg
     3481 cccgcgaccc gcggtggggg cgtctcgagg agacctacgg ggaggacccc gaactggtga
     3541 gccgcatggg cgtcgcctac gtgcgcggcg tgcagagcca gggggtcgac tgttgtggca
     3601 agcacttcct gggccacgcc agcacgctgg gcggcctgaa ccacggccag gtcgtgctcg
     3661 ggccgcggcg cctgcgggat gtcgaggcgg tcccgtttcg tgccgccatc cacgaggccg
     3721 gactggccac ggtcatgaac gcttacaacg acctcgacgg cctgcccatc gtggggtcgc
     3781 cggagatcat gaccgacctc ctccgcggcg agctgggctt cgagggctgc gtcgtcgccg
     3841 actactactc gatcgacgac ctcgacgggc tccaccacat cgtcgccgac cgcgaggagg
     3901 cggccaggat ggcgctgtcg gcgggcatcg acgtggagct cccgtcagcc gtgtactacg
     3961 ccacgctcgc cgaccaggtg cggtctggac ggctggacga ggcggtcgtc gaccaagcgt
     4021 gccgtcgggt cctcgaggag aagttggcgc tcgggttgtt cgacgagcgg tacgtcggca
     4081 ccgaggccgc cgacggcatc gagaccgtcg aggacgtcgc cctggcccgt cgggcctccg
     4141 cccggtcgat cgtgctgctg accaacgacg gcaccttgcc cctcgcgccg ggagcgcgcg
     4201 tcgcggtgct cgggccgtcg gccacggaca cccgccggct ctacggcgac tactcctacc
     4261 ccggccggtc catccacctc ggggtcgacc aggtcgtcgc ggacgtggtc gccccgaacg
     4321 acctccgccg gatcgcccgg cgggtgccgg caccccgcca ggcgctggct gagcgctacg
     4381 aggtcgtgga cgacctcgac ggcgccgacg tggccgtcgt gatcgtcggg ggccagagcg
     4441 gcatgtcgga ggaggacacc tccggggagt tccgcgacgc cagcgacctc cgcctgccgc
     4501 ccgagcagct ggacctcatc gcgacgaccg cggcctcggg tgtgccgacg gtcgtcgtcg
     4561 tggtcggcgg gcgagcccat tccctcgccg aggtcgtgcc ccacgccgca gccctggttc
     4621 tggcgtggct cccgggcgac gaggggggac ggggcctcgt cgacgtgctc tccggcgacg
     4681 tcgacgccgg cggtcggctc cccgtgtccc tgctgcaccg cgtcggccag gtcgggggct
     4741 accccgggca ccaccatggc ggcggacgaa gcctgatgta cggggactac gtcgacgggc
     4801 cggtggctcc cctgttcccg ttcggccacg gactctccta cacgagctgg tcctacgacg
     4861 atgtcgcggt ggccgccggg tcgaccacgg gccaaacggt gatcgacgtg gcgctcacca
     4921 acaccggcga tcgggagggc gaggaggtca ttcaggtctt cgcccgtgac gagctggcct
     4981 ccgtcggtgt cccggcccgg cgcctggttg cgttccagcg ggtgggagct gaggcggggg
     5041 agaccgtgcg cgttcgcttc acgatcccgg cgacgagtct cggcttccac ggcgtggatc
     5101 tccggttccg ggtcgagccc ggcgacgtca cgttcctcgt cggccccacc gccaccacgg
     5161 tgacggtgac cggcgatgtc gaacaccccg accccaacgc gccgcggccc ttcaccgtcg
     5221 acgtcacgag cgcctgaacg cgtcggtcag gacgccagct cgcggcggcg ctggtcccgg
     5281 agcacgaagc gctggacctt gccgctgggc gtcttcggca tctcgtcgat gaagatcacg
     5341 ttgcggggat aggcgtgggc ggccagcccc gtcttgacca gctgctggag ctcggcggcc
     5401 agctcggggc tgccggtgtg gcccgcgttg agcacgacgt gcgcctccag cacctcgccc
     5461 cggatctcgt cgggcacggc gatggccgcc gactccagca cggaggggtg gccgttgagc
     5521 acggactcga cctcgaacgg gccgatgcgg taccccgcca tgatgatgat gtcgtcgtcc
     5581 cgcgccgtga agtggatgtc gccgtcctcg tcgacgtagg ccgtgtcccc cgtgtggtac
     5641 cagcggcggt ccgcgctgaa cttcgcggcg gtggcctctg gcgcgtcctc gtagtggtgg
     5701 aaccaggcca aggggctggc ggtcatgtcg gcggcgatgc gtccgacgac accgggctcc
     5761 gcgggctcat cccggtccgt ctcgagcacg tggatcgacc agcccggcat cggccggccc
     5821 atgctcccgg gcttcagcgg ctgggcgacg tcggggtggt ggtggttgtt gaccagcatc
     5881 ccggcctcgg tctggccgta gtggtcgtgc acggtgaccc cgagggcgcc ggtcgcccat
     5941 tcgttgacgt ccggggtgag cggctcgccg gcgctcgacg cgcggcgcag cgccagctcg
     6001 ggcacgtcgt ccgacgcagc ccgtagggag cggtagacgg tgggggcggc ggcgaagttc
     6061 gtcacccggt gatcggcgag caccgcccag gcgacggcgg gggagaaggg ggcgtccagg
     6121 agcagggcgc aggcgccgag ggcgaggggg ccgacgatgg cgtagtagag gccgtaggcc
     6181 cagcccggat cggccccgca ccagaagacg tcgtcctcgc gcacgtcgaa cccgtactcc
     6241 atgtacgcct ggatggcagc gagggcgaaa gcgggcacga cgacgccctt cggggtaccg
     6301 gtcgtccccg acgtgaagat gtggatcatg ggggcgtcgc gcccgagggc ggccgccggg
     6361 aagcccgcct cctgggcgtc cacgaggtcc tggagcagga ggtcgccctc tcgtcgttcg
     6421 gcgccggggc cggcgacgac gaccgtccag tcggcatcct cccggatgtc ggtgccgggg
     6481 tcgagcttgt gccgctgccc gtcgtcgcac accacggcct tggtgtggct ggcttcgagc
     6541 cggaaggcga tcgcgggtgg ggcgaaggcc gtgaagagcg ggacgtggac cgccccgagg
     6601 cgccagatgg cgaccaccgt cgtcacgtag tccacgctct tgcccatcag ggtggcgatc
     6661 cggtcgccgg ggccgatccc gagctcggcg aaggccgccg ccagccgctc ggaggattcg
     6721 cggagctgtc cgtaggtcag cgtcacggcg cggaggtcgg cctccacaac ggtgaacgcc
     6781 ggcgcgtccg ccggatgtcg gtcgcagagc acgtgggcga ggcccaggtc ctccccgccg
     6841 tactcggcca ccagctcgtc gatgctcgcg gtcatgtcgt gcctcctgcg cccgctaaac
     6901 aggtgtttag cgctcgggct gcctgccgtc caatggtgcc actagaacgg cgtgctgctg
     6961 gagtgataac ctcgttctca tgagcgtcac gacccagcag atcactcccg agatcggcct
     7021 cgaggtgacc ggcctggccg gtcatgagct ggtcgacccc gaccgagcgg cggagtgcca
     7081 agccgccctg gacgcccacg gggtggtcgt ctaccgggag gctggcatcg gcgacgccga
     7141 cctcctggcc ttcaccgaga tgctcggcac cgtcgtcatc ccgaagatgg gtgggctgga
     7201 ggagcacccc ggggtc
//
LOCUS       DASTFV010000285         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1087387, whole genome shotgun sequence.
ACCESSION   DASTFV010000285 DASTFV010000000
VERSION     DASTFV010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087387"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VFP02_05395"
     CDS             complement(<1..1026)
                     /locus_tag="VFP02_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9542490.1"
                     /translation="MTADTEPTGEAAAEGPAATVQRRAPFSDNPNVGARGQRTQQRIL
                     DAALQVFGEVGYHRCSIDRIAKVAGCSRVSFYQYFSSKEDVFRHLAGQVARQVHASTE
                     ALGPIGPDGDGWTALRSWVARHSEIYERYEPVYQAFQAAAESDEAIAGGSVRTGEHTV
                     ALLRSRLATTTLPARQLDPLIHLLLECLTRTHDMAAILRSAAPDAYPRERVDDALTDV
                     IHRTLFGLYADVNVHHPAARRPSPLPFGPVMADVLQRDDEPPELTAAGRRTLDALLEA
                     GAEVLVRRGFHRARVDDIVAAAGVSHGAFYRYFENKEELAQILAVRAIRRVSTALAEI
                     PDAPTSDG"
     gene            complement(1049..1960)
                     /locus_tag="VFP02_05400"
     CDS             complement(1049..1960)
                     /locus_tag="VFP02_05400"
                     /inference="COORDINATES: protein motif:HMM:NF006140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET9542491.1"
                     /translation="MADDYQFLLYESIDDGRIVRIMLNRPEARNAQNRGMLVELNEAF
                     LRAEADDEVRVVILGGSGKMFSSGHDLGSKASRDENTPGPTMHPTRLVNGGTRKGAEA
                     LMLQEWHYFFENTRRWRNLRKITIGQAHGDVYAAALMLLWACDLIVAADDTTFADVVG
                     SRLGMCGVEYFGHPWEFGPRKAKELLLTGDSIDADEAHALGMVSKVFPADDLADRTLD
                     FARRIAAQPTMSSLLIKEAVNQTQDNMGFYTALNACFTLHELNHSHWAQVHDNGWPVA
                     LEEDGIPNWKEAPPIVPAVKDQVQAEG"
     gene            2026..>2306
                     /locus_tag="VFP02_05405"
     CDS             2026..>2306
                     /locus_tag="VFP02_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9542492.1"
                     /translation="MSRALVLGGGGPLGVGWEAGFLAGLADAGVLLEDADLVIGTSAG
                     SIVGAQLTSGRPLVDLVASVSEAPPWATGGSGDEPQDLGEMLAARAGGTT"
BASE COUNT          292 a          746 c          899 g          369 t
ORIGIN      
        1 gccgtcggac gtcggggcgt cggggatctc ggccagggcc gtcgacaccc gacggatcgc
       61 ccgcacggcc aggatctggg ccagctcctc cttgttctcg aagtagcggt agaacgcgcc
      121 gtgggacacc ccggccgcgg cgacgatgtc gtcgacgcgg gcccggtgga agccccggcg
      181 cacgaggacc tcggcgcccg cctcgaggag agcgtcgagg gtgcgtcggc ccgccgcggt
      241 cagttcgggg ggctcgtcgt cgcgctggag gacgtcggcc atcaccggcc cgaaggggag
      301 aggggacggt cgccgcgccg cgggatggtg cacgttcacg tcggcgtaga ggccgaacaa
      361 cgtgcggtgg atcacgtcgg tgagggcgtc gtccacccgc tcccgcgggt acgcgtcggg
      421 ggcggccgaa cgcaggatgg cggccatgtc gtgggtgcgg gtcaggcact cgagcaggag
      481 gtggatcagg gggtcgagct ggcgggccgg cagggtcgtg gtcgccagcc gggaccggag
      541 gagggccacg gtgtgttcgc cggtgcggac cgatccgccg gcgatcgcct cgtcgctctc
      601 ggccgccgcc tgaaaggcct ggtagacggg ctcatagcgc tcgtagatct cgctgtggcg
      661 ggccacccag ctgcgcagcg ccgtccagcc gtcgccgtcc ggaccgatcg gcccgagggc
      721 ttcggtcgac gcgtgcacct ggcgggcgac ttggccggcg aggtgccgga acacgtcctc
      781 cttgctggag aagtactggt agaaggacac ccgggagcac ccggcgacct tggcgatgcg
      841 gtcgatgctg caccggtggt agccgacctc gccgaagacc tggagggcgg cgtcgaggat
      901 ccgctgctgg gtgcgttggc cgcgagcccc gacgttgggg ttgtccgaga acggggcgcg
      961 ccgttgcact gtcgcggcgg ggccctcggc ggcggcctcg cccgtgggtt cggtgtcggc
     1021 ggtcatcggc gcgatgccgg tggttccgtc agccctcggc ttggacctgg tccttgaccg
     1081 cggggacgat gggcggggct tccttccagt tcgggatgcc gtcctcctcg agcgccaccg
     1141 gccagccgtt gtcgtggacc tgggcccagt gggagtggtt cagctcgtgg agcgtgaagc
     1201 aggcgttcag ggcggtgtag aagcccatgt tgtcctgggt ctggttgacc gcctccttga
     1261 tgaggagcga tgacatcgtc ggctgcgccg cgatccgccg ggcgaagtcg agggtgcggt
     1321 cggcgaggtc gtcggccggg aagaccttgg acaccatgcc gagggcgtgg gcctcgtcgg
     1381 cgtcgatgga gtctccggtg agcagcagct ccttggcctt ccgggggccg aactcccagg
     1441 ggtggccgaa gtactcgacg ccacacatgc ccaggcggga gccgaccaca tcggcgaagg
     1501 tggtgtcgtc ggcggccacg atgaggtcac aggcccacag cagcatcagc gccgcggcgt
     1561 acacgtcgcc gtgggcctgg ccgatggtga tcttgcgcag gttgcgccag cgccgggtgt
     1621 tctcgaagaa gtagtgccac tcctgcagca tcagggcctc ggcccccttg cgggtgccac
     1681 cgttgacgag gcgggtcggg tgcatggtcg gtcccggagt gttctcgtcc ctcgacgcct
     1741 tggagccgag gtcgtggccg gaggagaaca tcttgccgct accgcccagg atcacgaccc
     1801 ggacctcgtc gtcggcctcg gcccgcagga aggcctcgtt cagctccacg agcatcccgc
     1861 ggttctgcgc gttccgggcc tcgggccggt tcagcatgat ccggacgatc cgcccgtcgt
     1921 cgatggactc gtagagcagg aactggtagt cgtcggccat tggggtgact ctagtgttag
     1981 atgcggacgg cgtcggcggc gccgaggcct cactagcgtt cggccatgag ccgtgccctg
     2041 gtcctcggtg gtggtggccc gctcggtgtc ggctgggagg cgggcttcct cgcggggctg
     2101 gccgacgccg gtgtcctgct cgaggacgcc gacctggtca tcggcacctc ggccggctcg
     2161 atcgtgggcg cgcagctcac ctccggccgg ccgttggtcg acctggtcgc gtcggtctcg
     2221 gaggcgccgc cctgggcgac aggcggctcc ggggacgagc cgcaggacct gggcgagatg
     2281 ctggccgctc gcgccggcgg gaccac
//
LOCUS       DASTFV010000286         3984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1088607, whole genome shotgun sequence.
ACCESSION   DASTFV010000286 DASTFV010000000
VERSION     DASTFV010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088607"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VFP02_05410"
     CDS             <1..506
                     /locus_tag="VFP02_05410"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HET9542493.1"
                     /translation="EVGISFVYVTHDQEEALTMSDRLAVFDHGRIEQVGTPAEVYEFP
                     LTAFVAGFVGVSNVITGAAATTLTGSPDAFAVRPEKIRLGDLDTEAGSDECAVDGVVR
                     DVVYLGPHTRYLLSLDLGQDLTVVEQNLATTSGEVLAAKGRRVRALWGRAYNHPIHSG
                     DDQGENT"
     gene            503..1705
                     /locus_tag="VFP02_05415"
     CDS             503..1705
                     /locus_tag="VFP02_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9542494.1"
                     /translation="MKHYRLLAVLVAVLGLLTAACGDDSGGDDGGSTATPDTADLPTE
                     VGEGEGALNIIAWAGYIEDGSTEADIDWVTAFEDETGCAVEVTTAGTSDEMVSLMTNS
                     SDYDLVTASGDASLRLIAGETVQPVNLDLIPSYSTVDERLQGAPWYTIEDVAYGVPYQ
                     WGADVLMYNTDVFPEPPTSWGTIYSAQDFPDGESNAGRIQQYDGPISIATAALYVKAT
                     QPDLGIEDPYALNQEQYDAVIEVVRDSQPLVQRYWHDVVVQTEDFTTEGTAASSSWPY
                     QVNLLVDEPVSSVIPEEGTTGWADTTMLATNAPHPNCAYLWMEHSLDPTVQGDVAYWF
                     GSVPAVPAACEGNVLLTDEGCTTNGFDNFDQVAFWKTPEADCFGDGGECVPYSQWTED
                     YLAIQGGN"
     gene            1786..2733
                     /locus_tag="VFP02_05420"
     CDS             1786..2733
                     /locus_tag="VFP02_05420"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9542495.1"
                     /translation="MTATSAPGALDHLTVWHRLSAFFYRHRGLKVALLLTPPLLWFGV
                     FYLGSLVSLLWQSFYRLDDFSGTVDETLSLDTWKDLFTAANRDVILRTMAMAAAVTVA
                     CIALAYPIAYYMARRASTRMKALLFVLVLLPLWSSYLVRVYAWKLILAQEGVLTWFVD
                     HLGLSFLLDAALDLPTIGGTSLSVSPLGQWMVFTYIWLPYMILPVAAALERVPDSLED
                     ASSDLGAHPRTTFRKVIWPLAVPGVVAGSIFTFSLTLGDYIIPTVIGNSSPSIGESIY
                     SYQGAAGNLPLAAAFSVVPIVIMVVYLAVARRFGAFESL"
     gene            2730..3602
                     /locus_tag="VFP02_05425"
     CDS             2730..3602
                     /locus_tag="VFP02_05425"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9542496.1"
                     /translation="MSEGRKAGWGLKGTVVGVVVFLHFPLLIVILYAFTTQESAFSFP
                     PPGLTTRWFSQALDTPDLWAAMRLSVEVALVATVVAMVLGSMAAAAVGRFHFFGREGI
                     SVFLVLPLALPGIITGIAIRSALNLGDVPFSLWTIVIGHGTFCVVVVYNNALARLRQT
                     SGSVVEASQDLGASGVQTLRHVLLPTVGTALLAGGMLAFALSFDEIIVTQFTHGPGQE
                     TLPIWIFQSLFKPRDRPLTNVAAVFVIALTFIPILLAERMTRGAGVFPRQVADITPES
                     ESDAMLEPDPAVQD"
     gene            complement(3610..>3984)
                     /locus_tag="VFP02_05430"
     CDS             complement(3610..>3984)
                     /locus_tag="VFP02_05430"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9542497.1"
                     /translation="LRPVAVVLIEVVEGLPDSTPRAAAPRTVAESISATIRDADTACR
                     MLDGRFGLVLEDTPENGAIWTVERIRRHLAGVNTGMTLWAGVACYPAHAFDAGEILAQ
                     ADRALHAAREWRQDRIEVATSP"
BASE COUNT          561 a         1445 c         1323 g          655 t
ORIGIN      
        1 aggaggtcgg catcagcttc gtctacgtca cccacgacca ggaggaggcg ctgaccatga
       61 gcgaccgcct cgccgtgttc gaccacggcc gcatcgagca ggtgggcacc ccagccgagg
      121 tctacgagtt ccccctgacc gccttcgtgg ccggcttcgt cggggtctcc aacgtgatca
      181 ccggcgccgc cgccaccacg ctcaccgggt ctccggacgc ctttgccgtc cgtcccgaga
      241 agatccgcct gggcgacctc gacaccgagg ccgggtccga cgagtgcgcg gtcgacggcg
      301 tcgtgcgcga cgtcgtctac ctcggcccgc acacccgtta cctgctctcg ctcgacctcg
      361 gtcaggacct gaccgtcgtc gagcagaacc tcgccaccac ctcgggagag gtgctggccg
      421 ccaagggccg ccgcgtgcgg gccttgtggg gccgggcgta caaccacccg atccactcgg
      481 gtgacgacca aggggagaac acatgaagca ctaccggttg ttggccgtcc tggtggcggt
      541 cctcggcctg ctcaccgcgg cgtgcggcga cgacagcggg ggagacgacg gcggctcgac
      601 cgccacaccg gacacggccg acctgcccac tgaggtcggt gagggcgagg gggcgctgaa
      661 catcatcgcc tgggccggct acatcgagga cgggtcgacc gaagccgaca tcgactgggt
      721 caccgccttc gaggacgaga ccggctgcgc ggtcgaggtg accaccgccg gcacgtccga
      781 cgagatggtc tcgctgatga ccaacagcag tgactacgac ctggtcaccg cgtcaggtga
      841 cgccagcctg cgcctcatcg ccggggagac ggtccagccg gtcaacctcg acctgatccc
      901 gagctactcg acggtggacg agcgcctcca gggtgcgccc tggtacacga tcgaggacgt
      961 cgcctacggc gtgccctacc agtggggggc ggacgtgctg atgtacaaca ccgatgtctt
     1021 cccggagccc ccgacgtcgt ggggcaccat ctactcggcg caggacttcc ccgacggcga
     1081 gtccaacgcg ggccgcatcc agcagtacga cggcccgatc tccatcgcca ccgccgcgct
     1141 gtacgtgaag gccacccagc ccgacctcgg catcgaggac ccgtacgccc tgaaccagga
     1201 gcagtacgac gccgtgatcg aggtggtgcg tgactcccag ccgctggtcc agcgctactg
     1261 gcacgacgtg gttgtgcaga ccgaggactt cacgactgaa ggcacagcgg ccagcagctc
     1321 ctggccgtac caggtgaacc tgctggtcga cgagccggtc tccagtgtca tccccgagga
     1381 gggcacgacg gggtgggcgg acacgacgat gctggccacc aacgcgccgc acccgaactg
     1441 cgcctacctg tggatggagc actccctcga tcccacggtc cagggcgacg tggcctactg
     1501 gttcggctcg gtgccggctg tccccgcggc ctgtgaaggc aacgtgctgc tgaccgacga
     1561 gggctgtacc accaacggct tcgacaactt cgaccaggtc gcgttctgga agacgccgga
     1621 ggcggactgc ttcggggacg ggggcgagtg cgtcccctac tcgcagtgga ccgaggacta
     1681 cctggccatc cagggcggca actgaccgtt cgctgaccgg gcccgggcgg gtcgggtggg
     1741 gcctcggccc cgcccggccc ctcggcccga tcgacggatc ccccgttgac cgccacctcc
     1801 gcgcccggcg ccctcgacca cctgacggtc tggcaccgcc tgagcgcgtt cttctaccgc
     1861 caccgcggcc tgaaggtcgc gctcctcctc acgcccccac tcctgtggtt cggcgtcttc
     1921 tacctgggct cgttggtctc cctgctgtgg cagagcttct accggctcga cgacttcagc
     1981 ggcaccgtcg acgagacgct cagcctcgac acctggaagg acctgttcac cgccgccaac
     2041 cgcgacgtca tcctgcgcac gatggcgatg gctgcggccg tgaccgtggc ctgcatcgcc
     2101 ttggcctacc cgatcgccta ctacatggcc cgccgggcct cgacccggat gaaggccctc
     2161 ctcttcgtgc tggtgctgct cccgctgtgg tcgagctacc tggtgcgggt gtacgcctgg
     2221 aagctgatcc tggcccagga gggcgtgctc acctggttcg tcgaccacct cggcctgagc
     2281 ttcctgctcg acgccgccct cgacctgccg accatcggcg gcacgagcct ctcggtgtcc
     2341 ccgctcgggc agtggatggt gttcacctac atctggctgc cgtacatgat cctgccggtc
     2401 gccgccgccc tggagcgggt gccggactcg ttggaggacg cctccagtga cctcggggcc
     2461 cacccccgca ccacgttccg gaaggtgatc tggcccctgg ccgtccccgg tgtcgtcgcc
     2521 gggtcgatct tcaccttctc cctgaccctt ggtgactaca tcatcccgac ggtcatcggg
     2581 aactccagcc cgtcgatcgg cgagagcatc tacagctacc agggggcggc ggggaacctg
     2641 cccctcgccg ccgccttctc tgtggtgccc atcgtgatca tggtcgtcta cctggccgtc
     2701 gcccggcgct tcggcgcctt cgagtcgctg tgagcgaggg ccggaaggcg ggctggggcc
     2761 tcaagggcac cgtggtgggg gtggtggtgt tcctgcactt cccgctgctg atcgtgatcc
     2821 tctacgcctt cacgacccag gagagcgcgt tcagcttccc accccccggg ctcacgacgc
     2881 ggtggttcag ccaggccctc gacacccccg acctgtgggc ggcgatgcgc ctctcggtcg
     2941 aggtggcgct ggtggcgaca gtggtggcca tggtcttggg ctcgatggcc gccgccgccg
     3001 tcggccggtt ccacttcttc gggcgtgagg gcatctcggt gttcctcgtc ctgcccctgg
     3061 ctctgcccgg gatcatcacc ggcatcgcca tccgctcggc gctcaacctc ggcgacgtcc
     3121 ccttctcgct gtggacgatc gtcatcggtc atgggacctt ctgcgtggtc gtcgtctaca
     3181 acaacgccct cgcccgcctg cgccagacgt cgggctcggt ggtggaggcc agccaggacc
     3241 tcggggcctc cggcgtgcag acgttgcgcc acgtgctgct gccgacggtg ggaaccgcgc
     3301 tcctggccgg ggggatgctg gcgttcgccc tctcgttcga cgagatcatc gtcacccagt
     3361 tcacccacgg cccgggtcag gagacgctgc cgatctggat cttccagagc ctcttcaagc
     3421 cgagggaccg cccgctgacg aacgtcgccg cggtgttcgt gatcgccctg accttcatcc
     3481 ccatcctgct ggccgagcgc atgaccagag gcgccggcgt cttcccccgt caggtcgccg
     3541 acatcacccc cgagtccgag tccgacgcca tgctcgagcc cgaccccgca gttcaagact
     3601 gaggcgtggt cacgggctgg tagcgacctc gatgcggtcc tggcgccact cccgggcggc
     3661 gtggagggcc cggtcggcct gggccaggat ctcgccggcg tcgaaggcgt gggccgggta
     3721 gcaggccacg ccggcccaca gggtcatgcc ggtgttgacg ccggccaggt ggcgacggat
     3781 gcgctcgacg gtccagatgg cgccgttctc gggcgtgtcc tccaggacca ggccgaagcg
     3841 gccgtcgagc attcggcagg ccgtgtcggc gtcgcggatc gtggcgctga tgctttcggc
     3901 gaccgtgcgg ggggcggccg cccggggcgt gctgtccggc aggccctcga ccacctcgat
     3961 gaggaccacg gcgacggggc gcag
//
LOCUS       DASTFV010000287         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1088831, whole genome shotgun sequence.
ACCESSION   DASTFV010000287 DASTFV010000000
VERSION     DASTFV010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088831"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VFP02_05435"
     CDS             complement(<1..1024)
                     /locus_tag="VFP02_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET9542498.1"
                     /translation="MTGEPSEGGWGPLLDELRRRQGAARALGGAEKIGRQHAKGKLDA
                     RQRLERLFDPGSFTELGALVGGLDDLPGDGLVAGLGSVDGRPVLAAAEDFTVKGGSIG
                     VGNTDKRYRLTQIAGREGLPLVLLLDGAGHRMTGEAAGRRPNDLQGLADLKGRVPLVT
                     LVLGPAAGHSALAAPLADYSVMTEQAALFTAGPLLVKAATGEDVTPEALGGPQVAVAS
                     GVVHDVVADDAAAIDRARAWIGYFDGEVGDGDDVGPRALGDLLEVIPADPRRPYAMVE
                     VVDRLADVGSVLVTGAGFGGGMLTALIRLGGRSVAVVANDPTVRAGAIGRDEADKAGR
                     FLDTVAP"
     gene            1069..1851
                     /locus_tag="VFP02_05440"
     CDS             1069..1851
                     /locus_tag="VFP02_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET9542499.1"
                     /translation="MPDERVKLTYDGPVAVITNDNPDKKNAFDDEMDLALFEILGELK
                     ERKDVRAVVWRGEGSIWSSGRDVGAIGGGQVEMTHHELMTRGQKGIQQVWDIEAPIIV
                     AIQGWAIGGSFQRALLCDIRIAAEGARFMLPETTYGVIPDTGGVGVLFQMAGHGVVSD
                     MVLTGRPLSAEEALHHGIVSRVVAPEELDDTAMAMAHEIAGRSAVAVKMARKVIRHLG
                     HAQQRAAMEDELIYQTFINKSDDYAEQRAAHAEGRDPVFKGS"
     gene            1853..>2283
                     /locus_tag="VFP02_05445"
     CDS             1853..>2283
                     /locus_tag="VFP02_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9542500.1"
                     /translation="MSTAEPIGLPEPPELGSTALPDGTYAGQVVLVTGAGTGLGKAIA
                     TEFARLGAAIAILSRKPEHLDAGREAIEGVGGRALTVTCDIREPDQIAAALDTVEADQ
                     GLPDVLVNNAAANFPVPAEDMSANAWRTVVDITLNGTFFCAR"
BASE COUNT          337 a          870 c          800 g          276 t
ORIGIN      
        1 agggggcgac ggtgtcgagg aagcggcccg ccttgtcggc ctcgtcccgg ccgatggcgc
       61 cggcccgcac cgtcgggtcg ttggccacca cggccacgga acggccgccg aggcggatca
      121 gggcggtcag catcccgccg ccgaagcccg ccccggtcac caggacggac ccgacgtcgg
      181 ccagacgatc gacgacctcg accatcgcgt aggggcggcg gggatcggcc gggatcacct
      241 cgagcaggtc gcccagcgca cggggaccga cgtcgtcccc atcccccacc tcgccgtcga
      301 agtaaccgat ccaggcccgg gcccggtcga tggcggcggc gtcgtcggcc acgacgtcgt
      361 gcacgacgcc ggacgccacc gccacctgcg gccccccgag ggcttcgggc gtcacgtcct
      421 cgcccgtggc cgccttgacc agcaggggac cggcggtgaa cagggcggcc tgctcggtca
      481 tcaccgagta gtccgccagc ggggcggcca gggcgctgtg cccggcggcc ggcccgagga
      541 ccagggtcac caggggcacc cggcccttca ggtcggccag gccctggagg tcgttgggcc
      601 gtcgccccgc ggcctcgccc gtcatccggt gaccggcgcc gtcgagcagc aggaccagcg
      661 gcagtccctc ccgaccggcg atctgggtga gccggtagcg cttgtcggtg ttgccgacgc
      721 cgatggagcc gcctttgacc gtgaagtcct cggccgccgc cagtaccggc cgaccgtcga
      781 ccgacccgag gcccgcgacc aggccgtcgc cgggcaggtc gtccagtccc ccgaccagcg
      841 cccccagctc ggtgaacgag cccgggtcga agagtcgctc caggcgctgg cgggcgtcga
      901 gcttgccttt ggcgtgctgg cgcccgatct tctccgcccc gccgagggcg cgggccgcgc
      961 cctgacgccg ccgcagctcg tcgagcagcg gcccccagcc cccttccgat ggctcccccg
     1021 tcacggccta ggttctaccg gaccaggacc acgccacgag ggggacggat gccggacgag
     1081 cgggtgaagc tgacctacga cgggccggtc gccgtcatca ccaacgacaa cccggacaag
     1141 aagaacgcct tcgacgacga gatggacctc gccctgttcg agatcctggg cgagctgaag
     1201 gagcgcaagg acgtacgggc cgtggtctgg cggggcgagg gctccatctg gtcctcgggc
     1261 cgggacgtcg gcgccatcgg cggcggccag gtcgagatga cccaccacga gctgatgacc
     1321 cggggccaga agggcatcca gcaggtctgg gacatcgagg ccccgatcat cgtggccatc
     1381 cagggctggg ccatcggcgg ctcgttccag cgggcgctcc tgtgcgacat tcgcatcgcc
     1441 gccgagggcg cccgcttcat gctcccggag accacgtacg gggtcatccc cgacaccggc
     1501 ggcgtcgggg tcctgttcca gatggcgggc cacggcgtgg tcagcgacat ggtcctgacc
     1561 gggcgaccgc tcagcgccga ggaggcgttg caccacggca tcgtctcccg ggtcgtggcc
     1621 cccgaggagc tggatgacac ggccatggcc atggcccacg agatcgccgg ccgctcggcc
     1681 gtcgccgtga agatggcccg caaggtcatc cgccacctcg gccacgccca gcagcgggcg
     1741 gccatggagg acgagctcat ctaccagacg ttcatcaata agtccgacga ctacgccgaa
     1801 cagcgcgccg cccacgcgga ggggcgcgac ccggtgttca aggggtcctg agatgtcgac
     1861 cgccgagccc atcgggctgc ccgagccccc cgagctgggg agcaccgccc tcccggacgg
     1921 cacctacgcc ggccaggtcg tcctggtcac cggcgccggc accgggctgg gcaaggccat
     1981 cgccaccgag ttcgcccgcc tgggcgcggc catcgccatc ctcagccgga agcccgagca
     2041 cctcgacgcc gggcgggagg ccatcgaggg cgtcgggggc cgggccctga ccgtcacctg
     2101 cgacatccgc gagccggacc agatcgccgc cgccctcgac acggtcgagg ccgaccaggg
     2161 cctgcccgac gtgctggtca acaacgcggc cgccaacttc ccggtcccgg ccgaggacat
     2221 gagcgccaac gcctggcgca ccgtggtcga catcaccctc aacggcacct tcttctgcgc
     2281 ccg
//
LOCUS       DASTFV010000288         3541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1089828, whole genome shotgun sequence.
ACCESSION   DASTFV010000288 DASTFV010000000
VERSION     DASTFV010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089828"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VFP02_05450"
     CDS             complement(<1..632)
                     /locus_tag="VFP02_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542501.1"
                     /translation="MDAEDRELFDRSLRHATAAHTGEDLDAELDELGWGDALAVDPEA
                     AVALLFEHQGFANATSTGLDRVLTDALGAHPDAMVLPALGRTDPPGDGATVRGLGTAT
                     LRRAKTAAVVTTADGAGLTILRVDAAQLDLRPVDGIDPELGLVEVTGDAQGVEATPLD
                     LAHWEAVIAAGQRALAHELVGASRAMLGLAREHAVERIQFGVPIASFQAVR"
     gene            complement(718..1848)
                     /locus_tag="VFP02_05455"
     CDS             complement(718..1848)
                     /locus_tag="VFP02_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9542502.1"
                     /translation="MELDEFRTSLDAWLDENAEALAPDFEGSGTLDQQVAQLDKVKQH
                     AFDAGWLRWGWPERVGGLSGSPLLRGYLGEALTARDLIEPGLYSMIEVLVPTMVDYAP
                     PPLAAEMVPKMLRGDETWCQGFSEPGTGSNLGSLSCRATADGDTWRVSGQKVWTSLAQ
                     YSQRCVLLTRTGTAEEAHRGITALFVDMDSPGISVRPIETLHGVAEFAEVFFDDVVVP
                     VERTIGEVGGGWAVAMDLLPYERSTALWHRGALMHRRMDELLDELPEGALTAAEVGTV
                     FQQLYAFRARSRATQHRLHSGERLGPETSVDKVLLATAEQALYELVGDGLGDDVLFGD
                     DVAAQQWRSEFFYARAATIYGGSAEIQRNIIAKRLLDLGSDR"
     gene            complement(1854..2300)
                     /locus_tag="VFP02_05460"
     CDS             complement(1854..2300)
                     /locus_tag="VFP02_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9542503.1"
                     /translation="MTMDQGDIADIIEVEQLLARYAVGMTQDDIEAVIEVFTPDGTYS
                     AFGDTYTLADFPTLVAAAPKGLFLTGTPVLELDGDEGTGQQTLCFVDQTNHDMRIGWY
                     TDTYRRTSAGWRLRTRSMTFLRRSGTRDSGKAHDPRRPEPTTASNE"
     gene            complement(2303..3358)
                     /locus_tag="VFP02_05465"
     CDS             complement(2303..3358)
                     /locus_tag="VFP02_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HET9542504.1"
                     /translation="MAVPVSLPSPLEAVRDHDFHRLRIARVIDETADARSYVLDVPED
                     LRPAFGYEAGQFCTFRAWIDGEPHHRCYSMCSTPGVDAELQVTVKRVPGGLVSNWMYD
                     TLEAGHEIEATYPAGVFRLVAGDGDLVAFAAGSGITPVFSLVKAALANTRRRVHLFYA
                     NRDRDSTIFAAELDDLADRFPGRLDLVHHLDVDQGFVDDGTVAPFAWVGDDPDFYLCG
                     PTPFMDIVERSLLDQGMAPDRVHVERFAVPEPVDEPDAPSDGAAARITIEVGGRTESG
                     DHHPGTTLLQTARQFGLAAPSSCEAGSCATCMAQVVEGAVTMRVNNALTDDEVADGWV
                     LTCQSVPISPSVRVVYE"
BASE COUNT          501 a         1201 c         1322 g          517 t
ORIGIN      
        1 cggacggcct ggaacgaagc gatcgggacc ccgaactgga tgcgttcgac ggcgtgctcc
       61 cgggcgaggc cgagcatcgc cctcgaggcg ccgaccagct cgtgggccag ggcccgctga
      121 ccggcggcga tcactgcttc ccagtgggcc aggtcgaggg gagtggcctc cacgccctgg
      181 gcgtcgcccg tgacctcgac cagcccgagc tcggggtcga tgccgtcgac cggccgcaga
      241 tcgagctgcg cggcgtccac ccggaggatt gtgaggccgg cgccgtcggc cgtcgtcacc
      301 acggctgccg tcttcgccct cctcagcgtc gcggtgccca ggccgcggac cgttgccccg
      361 tcgccgggcg ggtcggtgcg gccgagggcg ggcaggacca tggcgtcggg atgcgcgccg
      421 agggcgtcgg tgaggacccg gtcgagcccg gtggaggtgg cgttggcgaa cccctggtgc
      481 tcgaacagga gggcgacggc ggcctctggg tcgacggcca acgcgtcccc ccagcccagc
      541 tcgtcgagct cggcgtcgag gtcttcgccg gtgtgcgcgg cggtggcgtg gcgcaagctc
      601 ctgtcgaaca gctcgcggtc ctcggcgtcc atcaccggtc gctcccggga cccggcgaac
      661 cttcggcaga actcaccctg gcggtgggtc tttgccgaag gttggcgggg gggacggtca
      721 ccggtcgctc ccgaggtcga ggagacgctt ggcgatgatg ttgcgctgga tctcggcgct
      781 gcccccgtag atggtcgcgg cccgggcgta gaagaactcg gatcgccact gctgggcggc
      841 gacgtcgtca ccgaagagga cgtcgtcgcc gagcccgtcg cccaccagct cgtagagcgc
      901 ctgctcggcg gtggccagca gcaccttgtc caccgacgtc tcgggtccga gccgctcgcc
      961 cgagtggagg cggtgctggg tggcgcggga tcgggcccgg aaggcgtaca gctgttggaa
     1021 gacggtcccc acctccgccg cggtcagcgc gccctcgggc agctcgtcga gcagctcgtc
     1081 catccgccgg tgcatgaggg cgccgcggtg ccacagggcc gtgctgcgct cgtaggggag
     1141 gaggtccatc gccaccgccc accccccgcc gacctcgccg atcgtgcgct cgacggggac
     1201 cacgacgtcg tcgaagaaca cctcggcgaa ctcagccaca ccgtgcaggg tctcgatcgg
     1261 gcgcacggag atcccgggtg agtccatgtc gacgaagagg gcggtgatcc cgcgatgggc
     1321 ctcctccgcc gtgcccgtcc gggtgagcag gacgcagcgt tgcgagtact gggccaggct
     1381 cgtccagacc ttctggccgg agacccgcca ggtgtcaccg tcagctgttg cgcgacagga
     1441 gagggagccc aggttgctgc cggtgccggg ctcggagaag ccctggcacc aggtctcgtc
     1501 gccgcggagc atcttcggca ccatctcggc ggcgagcggg ggcggggcat agtcgaccat
     1561 ggtcgggacc agcacctcga tcatggagta cagcccgggt tcgatgaggt cccgggcggt
     1621 gagggcctcg cccaggtagc cccgcagcag cggagagccg ctcagcccgc cgacccgctc
     1681 gggccatccc cagcgcagcc agccggcatc aaaggcgtgc tgtttgacct tgtccagctg
     1741 ggctacctgc tggtcgaggg tccccgagcc ttcgaagtcc ggggccaggg cctcggcgtt
     1801 ctcgtccagc caggcgtcga gggacgtccg gaactcgtcg agctccatga ccgctactcg
     1861 ttcgacgcgg tggtcggctc gggtcggcga gggtcgtggg ccttgccgga gtcacgggtc
     1921 ccgctgcggc gcaggaaggt catcgagcgg gtgcgcagcc gccagcccgc ggacgtgcgc
     1981 cggtacgtgt cggtgtacca gccgatgcgc atgtcgtggt tggtctggtc gacgaagcac
     2041 agggtctgct ggcccgtgcc ctcgtcgccg tccagctcga gcaccggtgt gcccgtgagg
     2101 aacaggccct tgggggcggc ggccaccagc gtggggaagt cggccaaggt gtaggtgtcg
     2161 ccgaaggcgc tgtaggtgcc atccggggtg aagacctcga tgacggcctc gatgtcgtcc
     2221 tgggtcatgc cgacggcgta ccgcgccagc agctgctcga cctcgatgat gtcggcgatg
     2281 tcgccctggt ccattgtcat cctcactcgt agacgacccg cacggacggg ctgatgggca
     2341 cggactggca ggttaggacc cacccgtcag cgacctcgtc gtcggtcagc gcgttgttga
     2401 cccgcatggt cacggccccc tcgacgacct gcgccatgca ggtcgcgcag ctgccggcct
     2461 cgcacgagga gggagcggcc aggccgaatt ggcgggcggt ctgcagcagg gtcgtgccgg
     2521 gatggtggtc cccggactcc gtgcggcccc cgacctcgat ggtgatccgg gccgccgccc
     2581 cgtccgacgg cgcgtcgggc tcgtcgaccg gctccggcac ggcgaaccgt tcgacgtgga
     2641 cccggtccgg cgccatgccc tggtccagca ggctccgctc cacgatgtcc atgaacggcg
     2701 tggggccgca gaggtagaag tcggggtcgt cgcccaccca cgcgaacggg gcgacggtgc
     2761 cgtcgtccac gaacccctgg tcgacgtcga ggtggtgcac gaggtcgagc cgcccgggga
     2821 accggtcggc caggtcgtcc agctcggccg cgaagatcgt gctgtcccgg tcgcggttgg
     2881 cgtagaagag gtggacccgg cggcgcgtgt tggccaaggc ggccttgacc agtgagaaga
     2941 ccggggtgat cccgctcccc gcggcgaagg ccaccaggtc accgtctccc gccacgaggc
     3001 ggaagacccc ggcggggtac gtcgcctcga tctcgtgccc ggcctcgagc gtgtcgtaca
     3061 tccagttgga caccagcccg cccggcaccc gcttcacggt gacctgcagc tcggcgtcga
     3121 cccccggcgt cgagcacatc gagtagcagc ggtggtgcgg ctcgccgtcg atccaggccc
     3181 ggaacgtgca gaactgcccc gcctcgtagc cgaaggcggg gcgcaggtcc tcgggcacgt
     3241 cgagcacgta ggagcgggcg tcagcggtct cgtcgatgac ccgagcgatc cggaggcggt
     3301 ggaagtcgtg gtcgcgcacc gcttccagcg gagaggggag cgacaccggg accgccatgg
     3361 acccggatga taatcgaatt ctcaataatg acaatgcgga tcgcgtcatt acactcggcg
     3421 gccgaggagc gtgctccatc tcgaggggga gaggacatgg aggggacgga gcggtggcgg
     3481 cggcggccgc tgccggagga cctggccggg cactaccgcg ccgaggggtg gtggaccgac
     3541 g
//
LOCUS       DASTFV010000289         3186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1091547, whole genome shotgun sequence.
ACCESSION   DASTFV010000289 DASTFV010000000
VERSION     DASTFV010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091547"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /locus_tag="VFP02_05470"
     CDS             <1..1499
                     /locus_tag="VFP02_05470"
                     /inference="COORDINATES: protein motif:HMM:NF026213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HET9542505.1"
                     /translation="ALALYREHRGDRPVRAVIYTHSHIDHFGGVKGVVRQEAVDRGDV
                     VVLAPQGFLAHAVAENVYAGTAMARRAVYMYGRHLARGPEGQIGCGLGQAASDGAVGL
                     IAPTRDVTHTGQVEVLDGVTIEFQLTPGTEAPAEMNFLFADHAALCVAENATHNLHNL
                     VTLRGALVRDPHEWARYLTEAIALFSDRTDVTFASHHWPTWGRERGREFLALQRDLYA
                     YLHDQTLRRLNQGLTGSEIAETFELPDALEASWHLRGYYGSVSHNVKAVYQRYLGWFD
                     GNPAHLWAHPPVAAATRYVAAMGGADAAIGQARAALADDDPRWAAEVLNHVVFDDPGN
                     ETATSLLAEIYDRLGQGAENATWRNFFLTGALELRSGVEAQPQAIATDLLSALSVDQV
                     FDALAIRLDGPRAAADGLASVIDWHITDLDVHHRVTVRNGVVVPEPLAGPPDDGAADA
                     RYALTRAELLPVLVGLTTVDPVDGDPAVLTDLQAHLDRFEPDFSIVTP"
     gene            1519..2490
                     /locus_tag="VFP02_05475"
     CDS             1519..2490
                     /locus_tag="VFP02_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HET9542506.1"
                     /translation="MIIDVAAYRDGKRCDDVVTLDQALRVSHHGEGFVWLGLYEPTQD
                     EFDVACRRFDLHELAVEDAIKAHQRPKLEVYGPTVSMVLKTARYLDAEEEVEFGEIFL
                     FVGADFVVAVRHGEASALGDVRRELEGRPDRLTCGPSAVLHAIVDRVVDDYLPVLEGI
                     DTDIEEVEEQVFSRAGENPVERIYNLKREVLDFHRATVPLVDPVERLAEGKVPNVHGT
                     IQPYFRDVHDHLVRVTERVVADRDLLNSVLEANLTQVGIRQNEDMRKISAWVAIGVVP
                     TLLAGIYGMNLRHMPLLEHDAAFPIVVGVMVGIAVGLFALFRRRDWL"
     gene            complement(2503..>3186)
                     /locus_tag="VFP02_05480"
     CDS             complement(2503..>3186)
                     /locus_tag="VFP02_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9542507.1"
                     /translation="VIFTMVQPLFFLALFGPLLISVTGAGTAATLQTFVPGILVMSAL
                     FAASTTGANLLLEIQTGSHERMLVTPLSRSSLLLGRALKEIVPLVAQASIIVAVVIPF
                     GFRLELGGAVIGMAMLAVFGVGLGALSYTLALAVQSEDWIFWSVQQTLIFPLLLLSGM
                     LLPLDDAPGWLQALSKINPLTHIVDAERALFAGDVTDPVVLWGALSAAGLALVGLTVG
                     IRAMQRATN"
BASE COUNT          507 a         1191 c         1057 g          431 t
ORIGIN      
        1 cggccctcgc cctctaccgg gagcatcgag gggatcggcc ggtgcgagcg gtcatctaca
       61 cccacagcca catcgaccac ttcggcgggg tcaagggggt cgtccgccag gaggcggtcg
      121 accgcggcga cgtcgtcgtc ctggctcccc aggggttcct ggcccacgcc gtggccgaga
      181 acgtctacgc gggtaccgcc atggcccgcc gcgccgtcta catgtacggc cgtcacctgg
      241 cccggggccc ggagggccag atcggctgcg ggctcggcca agccgcatcg gacggggcgg
      301 tcggcctcat cgccccgacg cgggacgtca cccacaccgg ccaggtcgag gtcctcgacg
      361 gcgtgaccat cgagttccag ctcacccccg ggacggaggc cccggcggag atgaacttcc
      421 tcttcgccga ccatgccgcg ctctgcgtgg ccgagaacgc cacccacaac ctccacaacc
      481 tggtcaccct gcggggcgcg ctggtccggg acccccacga atgggcccgc tacctgaccg
      541 aggccatcgc cctgttcagc gaccgcacgg acgtgacctt cgcctcgcac cactggccga
      601 cctggggccg tgagcggggc cgcgagttcc tggccctgca acgggacctc tacgcctacc
      661 tccacgacca gaccctgcgc cgcctcaacc agggcctgac cggcagcgag atcgccgaga
      721 cgttcgagct gcccgacgcc ctcgaggcga gctggcacct ccggggctac tacggctcgg
      781 tcagccacaa cgtgaaggcc gtctaccagc gctacctcgg gtggttcgac gggaacccgg
      841 cccacctgtg ggcccacccg ccggtcgccg ccgccacccg gtacgtcgcc gccatgggcg
      901 gggccgacgc cgccatcggc caggcacggg cggcgctggc cgacgacgac ccgcgctggg
      961 cggccgaggt gctgaaccac gtcgtcttcg acgacccggg caacgagacg gcgacgtcac
     1021 tcctggccga gatctacgac cggctcggcc agggcgcgga gaacgccacc tggcggaact
     1081 tcttcctcac gggcgccctg gagctgcgtt ccggggtgga ggcccagccc caggcgatcg
     1141 ccaccgacct gctctcggcc ctctcggtgg accaggtgtt cgatgcgctg gccatccgcc
     1201 tcgacggtcc ccgagcggcc gcagacgggc tcgcctcggt catcgactgg cacatcaccg
     1261 acctcgacgt ccaccaccgg gtgacggtcc gcaacggggt cgtcgtcccc gagccgttgg
     1321 ccggacctcc cgacgacggt gccgccgacg cccgctatgc gctgaccagg gcggagttgc
     1381 ttcccgtgct cgtcggcctc accaccgtcg acccggtcga cggcgatccc gccgtcctga
     1441 ccgacctcca ggcccacctc gaccgcttcg agcccgactt ctcgatagtg acgccgtgaa
     1501 atctgggacc attccttcgt gatcatcgac gtggccgcgt accgggacgg aaagcgctgc
     1561 gacgacgtgg tcaccctcga ccaggcgctc cgggtcagcc accacggcga ggggttcgtc
     1621 tggctcggcc tctacgagcc cacccaggac gagttcgacg tcgcctgccg ccgcttcgac
     1681 ctccacgagt tggccgtcga ggacgccatc aaggcccacc agcgccccaa gctcgaggtc
     1741 tacggcccca cggtctccat ggtgctcaag acggcgcgct acctcgacgc cgaagaagag
     1801 gtggagttcg gcgagatctt cctcttcgtc ggcgccgact tcgtcgtcgc cgtccgccac
     1861 ggcgaggcca gcgccctcgg cgacgtccgg cgcgagctcg agggtcgtcc ggaccggctg
     1921 acctgcgggc ccagcgccgt cctccacgcc atcgtcgacc gtgtcgtcga cgactacctg
     1981 ccggtgctcg aagggatcga caccgacatc gaggaggtcg aggagcaggt cttctcccgg
     2041 gccggcgaga accccgtcga gcgcatctac aacctcaagc gggaggtcct cgacttccac
     2101 cgggcgaccg tgcccctggt cgaccccgtc gagcgcctgg ccgagggcaa ggtgcccaat
     2161 gtccacggga cgatccagcc ctacttccgc gacgtccacg accacctcgt tcgcgtcacc
     2221 gagcgggtcg tcgccgaccg ggacctgctc aacagcgtcc tggaggccaa cctcacccag
     2281 gtcgggatcc gtcagaacga ggacatgcgc aagatctccg cctgggtggc catcggcgtg
     2341 gtgcccaccc tcctcgccgg catctacggg atgaacctcc gccacatgcc cctgctcgag
     2401 cacgacgcgg ccttcccgat cgtggtcggc gtgatggtcg ggatcgccgt ggggctcttc
     2461 gccctcttcc gacggcgcga ctggctctga gccgaccatc ggtcagttcg tggcccgctg
     2521 catggcccgg atgccgacgg tgaggccgac cagggccagc cccgcagccg acagggcccc
     2581 ccagaggacg accgggtcgg tcacgtcgcc ggcgaacagg gcccgctcgg cgtccacgat
     2641 gtgggtcagg gggttgatct tggacagggc ctgcagccaa ccgggcgcgt cgtcgagggg
     2701 caggagcatc ccggacagga gcagcagcgg gaagatgagg gtctgctgga ccgaccagaa
     2761 gatccagtcc tcggactgca cggccaaggc cagcgtgtag gacagcgcgc cgaggccgac
     2821 accgaagacg gcgagcatcg ccatcccgat caccgccccg ccgagctcca gccggaagcc
     2881 gaaggggatc accaccgcca cgatgatcga ggcctgagcg accaggggca cgatctcctt
     2941 cagcgcccgg cccaggagca gcgaggaccg gctcagcggg gtcaccagca tccgctcgtg
     3001 ggagccggtc tggatctcca gcaacaggtt ggcgcccgtc gtggatgcgg cgaacagcgc
     3061 ggacatgacc aggatcccgg gcacgaaggt ctgcagcgtg gcggccgtcc ccgcgcccgt
     3121 gacggagatc agcagggggc cgaagagggc caggaagaag agcggctgga ccatcgtgaa
     3181 gatgac
//
LOCUS       DASTFV010000290         2923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1094369, whole genome shotgun sequence.
ACCESSION   DASTFV010000290 DASTFV010000000
VERSION     DASTFV010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094369"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VFP02_05485"
     CDS             <1..1156
                     /locus_tag="VFP02_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET9542508.1"
                     /translation="NFDLDYHLRFVKAANDGSMRELFDIAEPIGMQAFDRARPLWEFT
                     VVEGLEEGRAGLIMKIHHAVTDGVGGVKLMLETFDAEPDPGEPGDLPEAPAVHVMGQA
                     ERFADAVAHEQRRVMGVAKRSFGTLSTAALVTVADPVGAGARVSETVASALRLLAPAS
                     EPLSPLMTERSLSVQFHTLTVPLAEMKIAGRRAGGKLNDAFVAATASGWRRYHDHHDK
                     PAGALRMSMPINVRTAATENVAGNQFAPARFPVPLTVVDPVHRMKVVRDLVGRARAEP
                     ALALTEPLANLLNHLPPDVTTGIFGSMLRGVDFVTSNVPGPPFPIYLSGARVISQFPF
                     GPMAGAAANITLLSYVDELNIGINTDPSAVPDPETLVACMQEGFDEILKC"
     gene            complement(1158..2192)
                     /locus_tag="VFP02_05490"
     CDS             complement(1158..2192)
                     /locus_tag="VFP02_05490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET9542509.1"
                     /translation="MKISTQLRYDVSPAEVAQQAVDLEAAGLDLIWVAEAYGVDAVSI
                     MGYLAAVTERVEIASGILPLYTRTPTLLAMTAAGVDALSGGRCVLGLGASGPQVIEGF
                     HGVPYDKPLTRTREIIEICRQVWKRERVEFSGKAYAIPLPEGQGTGLGKPLKLITHPV
                     RDRIPIHVAALGPKNVELTAEIADGWLPLFYLPEKATDVWGDDLARGAAKRSDDLDPL
                     EVVAGGLVAIGPTDEVKGFRDFARPMVALYVGGMGARGKNFYNDLVCRYGFEKEAKEI
                     QDLYLDGKKEEAAAVIPEDLLEATTICGDEGYVKERLAAYRESGVTMLNIQPIGNPTE
                     IVSQLKDWTA"
     gene            complement(2204..>2923)
                     /locus_tag="VFP02_05495"
     CDS             complement(2204..>2923)
                     /locus_tag="VFP02_05495"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HET9542510.1"
                     /translation="VVGAEAHTVDLADPDEPVGLLAAVRPDVVIHTAYGTADLERDVV
                     TATRSVAAACAIRDLALVHLSSDVVFDGEHAPYAEDAPPVPVHAYGRAKAQAEADVAT
                     AVSDAAICRTSLVCAADPLDPRSTWVVDTLRTGEAIALFTDEIRCPIRADDLAGQVWD
                     VVALPRADRSGPWHLVGPDALSRYDLGVVLAEAHGLDPAGITAAASRDLPEPRPRDLR
                     LTAGRAAVLPTRPRPVGTLWH"
BASE COUNT          417 a         1049 c         1029 g          428 t
ORIGIN      
        1 caacttcgac ctcgactacc acctgcgctt cgtgaaggcg gccaacgacg gcagcatgcg
       61 cgagctgttc gacatcgccg agcccatcgg catgcaggcc ttcgaccggg cccggccgct
      121 gtgggagttc accgtggtcg aaggcctcga ggagggccgg gccgggctga tcatgaagat
      181 ccaccacgcg gtgaccgacg gcgtgggcgg cgtgaagctg atgctggaga ccttcgacgc
      241 cgagcccgac cccggcgagc ccggcgacct gcccgaggca ccggccgtac acgtcatggg
      301 ccaggccgag cgcttcgccg acgccgtggc ccacgagcag cgtcgggtca tgggggtggc
      361 gaagcgctcc ttcggcacgc tctccacggc cgcgctggtc accgtcgccg acccggtcgg
      421 cgccggggcc cgggtgagcg agacggtcgc gtccgccctc cggttgctgg cgcccgccag
      481 cgagcccctg tccccactga tgaccgagcg ctccctgtcg gtgcagttcc acaccctgac
      541 ggtccccctg gccgagatga agatcgccgg ccggcgggcg ggcggcaagc tcaatgacgc
      601 cttcgtggcc gccaccgcct cgggctggcg ccgctaccac gaccaccacg acaaaccggc
      661 cggtgcgctg cggatgtcca tgccgatcaa cgtgcgcacc gcggcgacgg agaacgtggc
      721 cgggaaccag ttcgcgccgg cccgcttccc cgttcccctg acggtggtcg atcccgtcca
      781 ccggatgaag gtggtgcggg acctcgtcgg gcgggctcgg gccgagcctg cgctggcctt
      841 gaccgagccc ttggccaacc tgctcaacca cctgcccccg gacgtgacca ccggcatctt
      901 cggctcgatg ctgcggggcg tggacttcgt gacctcgaac gtccccggcc cgcccttccc
      961 gatctacctg agcggggccc gggtgatcag ccagttcccc ttcgggccaa tggccggcgc
     1021 cgccgccaac atcaccctgc tcagctacgt cgacgagctg aacatcggca tcaacaccga
     1081 cccctccgcc gtccccgacc ccgagacgtt ggtcgcgtgc atgcaggaag gcttcgacga
     1141 gatcctcaag tgctgaacta cgcagtccag tccttgagct ggctgacgat ctcggtgggg
     1201 ttgccgatgg gctggatgtt gagcatggtc acgcccgact cgcggtaggc ggccaggcgc
     1261 tccttcacgt agccctcgtc gccgcagatg gtggtggcct cgagcaggtc ctcggggatg
     1321 accgccgcgg cctcctcctt cttgccgtcg aggtacagat cctggatctc cttggcctcc
     1381 ttctcgaagc cgtaccggca gaccaggtcg ttgtagaagt tcttgccccg ggcgcccatg
     1441 ccgcccacgt acagggcgac catcggacgg gcgaagtccc ggaagccctt cacctcgtcg
     1501 gtcgggccga tggccacgag accgccggcg acgacctcca gcgggtcgag gtcgtcggac
     1561 cgcttggccg cgccccgagc caggtcgtca ccccagacgt ccgtcgcctt ctcgggcagg
     1621 tagaagagcg ggagccagcc gtcggcgatc tcggccgtca actcgacgtt cttggggccg
     1681 agggcggcga cgtggatcgg gatgcggtcc cgaacggggt gggtgatcag cttcagcggc
     1741 ttgcccagcc cggtgccctg cccctccggg agggggatgg cgtaggcctt gcccgagaac
     1801 tcgacccgct cccgcttcca gacctggcgg cagatctcga tgatctcccg cgtccgggtg
     1861 agcggcttgt cgtaaggcac accgtggaag ccctcgatga cctgaggccc ggatgccccg
     1921 aggccgagta cgcagcgacc gcccgacagg gcgtcgaccc cggccgcagt catggccagg
     1981 agcgtcggcg tgcgggtgta gaggggcagg atccccgagg cgatctcgac ccgctcggtg
     2041 accgccgcga ggtaacccat gatgctgacc gcgtcgacgc catacgcctc ggccacccag
     2101 atgaggtcga gcccggccgc ctcgaggtcc acggcctgct gggccacctc ggcgggcgag
     2161 acgtcgtagc ggagctgggt gctgatcttc atggcgttcg aacctagtgc cagagggtcc
     2221 cgaccggcct aggccgggtg ggcagcacgg cggcccttcc ggcggtgagc ctcaggtccc
     2281 ggggacgggg ctcggggagg tcccggctcg ccgccgcggt gatccccgca gggtccaggc
     2341 cgtgggcctc ggccaggacg acgccgaggt cgtagcggct gagggcgtcc ggtccgacca
     2401 ggtgccacgg cccgctccgg tcggcgcggg gcagggccac gacgtcccag acctggccgg
     2461 ccaagtcgtc ggcccggatc gggcaccgga tctcgtcggt gaacagggcg atggcctcgc
     2521 ccgtccggag ggtgtctacg acccaggtcg accgcgggtc gagtggatcg gcggcgcaca
     2581 ccagcgacgt gcggcaaatg gcggcgtcgg acacggccgt cgccacgtcg gcctcggcct
     2641 gcgctttggc tcggccatag gcgtggaccg ggaccggggg cgcgtcctcg gcgtacggcg
     2701 cgtgctcgcc gtcgaagacc acgtcactgc tgagatggac cagcgccaag tcgcggatgg
     2761 cgcacgcggc ggcgaccgag cgcgtcgccg tcaccacgtc ccgctcgaga tcggctgtcc
     2821 cgtaggcggt gtggatgacg acgtcgggcc gtaccgccgc caacaggccg acgggctcgt
     2881 ccgggtcagc caggtcgacc gtgtgggcct cggctccgac aac
//
LOCUS       DASTFV010000291         3556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1097304, whole genome shotgun sequence.
ACCESSION   DASTFV010000291 DASTFV010000000
VERSION     DASTFV010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097304"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VFP02_05500"
     CDS             <1..230
                     /locus_tag="VFP02_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542511.1"
                     /translation="QGVVDDYRTLAHPWGVSVGATAPVTIWQGDADTMVPLRHAEALA
                     EHLPEAGLTVWPGEGHLGPVTHIDEILDSLI"
     gene            complement(345..1490)
                     /locus_tag="VFP02_05505"
     CDS             complement(345..1490)
                     /locus_tag="VFP02_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HET9542512.1"
                     /translation="MAPTNDDVLEALRAVQEPLLERSLVDLGLVDATEVHRGGRIKVS
                     IAEPTEDYPYRDQLRQAVSASVGALDGVKDVQIDARVLDDDGQERVRRLLIGDPEATA
                     GSQESHGHAEGRAIPFADVGSKTRVLLIASGKGGVGKSSVSANLAIALAQRDKRVAIV
                     DADVWGFSIPRMLGVTRPPILIDRMLVPPEANGVRCISMGFFAEEDTPVIWRGPMLHK
                     ALEQFLTDVYWGDPDFLLVDLPPGTGDIAISLAGFLPRAEVYVVTTPQAAAQKVAQRA
                     AFMAEKVRLEVRGVIENMSWFTGDDGKRYEIFGAGGGQELADRLDVPLVGQVPLVPEL
                     RAGSDSGRPIAAEAPDSEAAAVFRAMAERIDTELAPTKRYHPELKIT"
     gene            1559..2287
                     /locus_tag="VFP02_05510"
     CDS             1559..2287
                     /locus_tag="VFP02_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9542513.1"
                     /translation="MEGGAGGPLSPTEFASAVTAIASAFGDPTRRDIFLHVHQRTDGV
                     TASEVADRFDLHANVARHHLDKLAAGGYLEVSVERPDGGGAGRPSKRYRPTEHEMNLE
                     INVRHDDVLVTLLGRALEALPPAQAEALAEEVGEEYGRTMAGALGAESSGPRSFRTAL
                     HAVADALTAHGFAAHAEKHGNELRIVSEHCPFGGAAVEHPVICAVDRGMVKGMLGALY
                     GDTSPVTESSRPTGDEICVTAVAE"
     gene            2298..3410
                     /locus_tag="VFP02_05515"
     CDS             2298..3410
                     /locus_tag="VFP02_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HET9542514.1"
                     /translation="MRYYLDHASTSTLRPEARAAMVAALDLVGDPGRIHEEGLTVRVA
                     VEQARDQVASTFGARPREVVFTGSATEAVATVARGVAGRGRHQVVSAVEHSAVRLAAE
                     RAGEVTVVPVDRHGRVDPEEVAVAVRPDTAVVHVQAGNHEVGTTQPLAAVVTACAGLD
                     VLVHVDAAQANGHLPLDFATLGADLVSLSAHKFGGPAGVGALLVRRGLRLDPLLVGGD
                     QERARRAGLENVGAVTGFAAACGALAGGGLEAEATEARRLTDRVLHGVAGLDGIPIYG
                     DPVERLPHVVCLGIEGVEPQAVLLGLDAAGIAVHSGSACSSEALEPSPVLEAMGVDAH
                     RSLRVSVGWSSTDTDIDAFLTTLPRIVEDLRALGAG"
BASE COUNT          463 a         1316 c         1267 g          510 t
ORIGIN      
        1 cccaaggcgt ggtcgacgac taccggacgc tcgcccaccc ctggggcgtc tccgtcgggg
       61 cgaccgctcc cgtgacgatc tggcagggcg acgccgacac gatggtcccc ctccgccacg
      121 ccgaagccct ggccgagcac ctaccggaag ccggcctcac cgtctggccc ggcgaaggcc
      181 acctcggccc tgtcacccac atcgacgaga tcctggactc gctcatctga ggcggtcccg
      241 ccggtcccga cgctgcccag cggagcggag cagctcgctg atccactgct ccggcggagc
      301 cggacttgct gagcgcagcg aagccagtcg aatgtgacga ccacctacgt gatcttgagc
      361 tcggggtggt agcgcttggt gggggcgagc tcggtgtcga tgcgctcggc catggcccgg
      421 aagacggcgg cggcctcgct gtcgggggcc tcggccgcga tgggacggcc cgagtcgctg
      481 cccgcccgca gctccggcac cagggggacc tggccgacca gggggacgtc gaggcggtca
      541 gccagctcct ggccgccccc ggccccgaag atctcgtagc gcttcccgtc gtcgccggtg
      601 aaccacgaca tgttctcgat gacgccccgc acctcgagcc gcaccttctc ggccatgaac
      661 gcggcccgct gcgccacctt ctgggcggcg gcctgtggcg tggtcaccac gtagacctcg
      721 gcccggggca ggaagccggc gagcgagatg gcgatgtcgc ccgtgcccgg tgggaggtcg
      781 acgagcagga agtcgggatc gccccagtag acgtcggtca ggaactgctc gagggccttg
      841 tgcagcatcg ggccccgcca gatgaccggc gtgtcctctt cggcgaagaa gcccatggag
      901 atgcagcgca cgccgttggc ctcgggcggc accagcatcc ggtcgatcag gatcgggggt
      961 cgggtcacgc ccagcatccg ggggatcgag aagccccaga cgtcggcgtc gacgatggcc
     1021 acccgcttgt cccgctgggc cagggcgatg gccaggttgg cgctgaccga ggacttgccg
     1081 acgccgccct tgcccgatgc gatgagcagc acccgggtct tggagccgac gtcggcgaag
     1141 gggatggccc ggccctcggc gtgaccgtgg ctctcctggc tgccggccgt cgcctccgga
     1201 tcgccgatga gcagacggcg gacccgctcc tggccgtcgt cgtcgagcac gcgggcgtcg
     1261 atctgcacgt ccttgacgcc gtccagcgcc cccaccgagg cgctcaccgc ctgacggagc
     1321 tggtcccggt acgggtagtc ctcggtcggc tcggcgatgc tgacctttat ccgcccaccc
     1381 cggtggacct cggtcgcgtc gaccagtccg aggtcgacca ggctgcgctc gagcaggggt
     1441 tcctggacgg cgcgcagcgc ctccaggacg tcgtcgttgg tgggggccat cggtaccgat
     1501 ccgctcctgc gcgccggact cgggaatttc tcctatgcgc gtaggtagaa taccgggcgt
     1561 ggaaggtggt gctggtggtc ccctgtcccc caccgagttc gcgtcggccg tcacggccat
     1621 cgcctcggcc ttcggtgacc cgacccgccg cgacatcttc ctccacgtcc accagcgcac
     1681 ggacggggtg accgcttccg aggtggccga ccgcttcgac ctgcacgcca acgtggcccg
     1741 gcaccacctc gacaaactgg ccgccggcgg gtacctcgag gtcagcgtcg agcgtccgga
     1801 cggcggtggc gccggacggc cgtccaagcg ctaccggccg accgagcacg agatgaacct
     1861 cgagatcaac gtccgtcacg acgacgtgct ggtgacgctg ctcggacggg ccctcgaggc
     1921 cctgcccccc gcccaggccg aggccctggc ggaggaggtg ggcgaggagt acggccgcac
     1981 catggcgggc gcgctcggcg ccgagagcag cgggccccgt tccttccgga ccgccctcca
     2041 tgccgtcgcc gatgcgctga ccgcccacgg tttcgccgcc cacgccgaga agcacggcaa
     2101 cgagctgcgc atcgtctccg agcactgccc cttcggcggt gccgcggtcg agcacccggt
     2161 catctgcgcc gtggaccggg gcatggtcaa gggcatgctc ggcgccctct acggcgacac
     2221 ctcgcccgtg accgagtcgt cccgacccac cggcgacgag atctgcgtga ccgcggtggc
     2281 cgagtagccc gctgagggtg cggtactacc tcgatcacgc gtcgacatcg accctgcgtc
     2341 cggaggcccg ggcggcgatg gtggccgcct tggacctggt gggcgacccc ggccggatcc
     2401 acgaggaggg cctgaccgtc cgggttgccg tggagcaggc ccgggaccag gtggcgtcca
     2461 ccttcggggc ccggccccgg gaggtggtgt tcacgggcag cgccacggag gccgtcgcca
     2521 cggtggcccg gggcgtcgcc ggccgaggtc ggcaccaggt cgtcagcgcg gtcgaacact
     2581 ccgcggtccg tctggcggcc gagcgggcgg gcgaggtcac cgtggtcccg gtggaccggc
     2641 acggtcgggt cgatccggag gaggtggccg tcgccgtgcg tccggacacc gccgtcgtcc
     2701 acgtgcaggc cggcaaccac gaagtcggga cgacccagcc cctggccgct gtcgtcaccg
     2761 cctgcgccgg gctcgacgtg ctcgtccacg tcgacgccgc ccaggccaac ggccaccttc
     2821 cgctggactt cgccacgctc ggtgccgacc tcgtgtccct cagcgcccac aagttcggcg
     2881 gccccgcagg agtcggcgcg ctgctcgtgc gccgcgggct gcgcctcgac ccgctcctcg
     2941 tcggtggcga tcaggagcgg gcccgccggg ccggcctgga gaacgtgggc gccgtcaccg
     3001 ggttcgccgc ggcctgcggt gcgctggccg gcggcggtct cgaggccgag gccacggagg
     3061 cccgccgtct gaccgatcgg gtcctgcacg gcgttgccgg tctggacggc atcccgatct
     3121 acggtgaccc ggtggagcgc ctcccccatg tcgtgtgcct cggcatcgag ggagtcgagc
     3181 cccaggccgt gctcctcggg ctcgatgccg cggggatcgc cgtgcactcg ggcagcgcct
     3241 gctcgtcaga ggccctggag ccgtcaccgg tcctggaggc gatgggcgtc gacgcccacc
     3301 gctccctccg ggtctcggtg ggctggtcca gcaccgacac ggacatcgac gccttcctca
     3361 ccacgctccc ccggatcgtc gaggacctcc gggccctggg ggcgggttga gcctgctgct
     3421 cgtccgccac gtcgacgctg tctcccggaa cgattggccc gccgacgacc ggctacggcc
     3481 gctgaccgag aaggggttcg atcaggcccg ggccctcgtc gacctcctgg gtgggcaccg
     3541 tgtcgaggtc atcctc
//
LOCUS       DASTFV010000292         3106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1097624, whole genome shotgun sequence.
ACCESSION   DASTFV010000292 DASTFV010000000
VERSION     DASTFV010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097624"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..452
                     /locus_tag="VFP02_05520"
     CDS             210..452
                     /locus_tag="VFP02_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HET9542515.1"
                     /translation="MKDPEQYEALRDEGMSKEKAARISNASANEGRSSVGRKGGEAED
                     YEDRTVDELRDRAAEIGIEGRSDMTKDELIDALRNH"
     gene            478..834
                     /locus_tag="VFP02_05525"
     CDS             478..834
                     /locus_tag="VFP02_05525"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9542516.1"
                     /translation="MTDELLALERGAWEALATDGEAATAFYDRVLADDVLMLLPGDMV
                     IDDRATVVSSMSGAAWQSYELTDERVVPLGEDSAVVGYRGTATRDDTPYRALFNSTYV
                     REDGDWKLVLHQQTPV"
     gene            complement(831..1343)
                     /locus_tag="VFP02_05530"
     CDS             complement(831..1343)
                     /locus_tag="VFP02_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_273701426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1068..1070),aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HET9542517.1"
                     /translation="MSVLLDPTGESAPAPRPLLDRPATLEGLTVGLLDISKPRGDVFL
                     DQLESHLRARGATVERFAKPTFTKPAPADLRYEIAQRCHVVIEALADUGSCTSCSVHD
                     IADLERRGIPGAFIASTEFIEAAETQADALRFPVARVFVPHPIQDRTDDEMRALADAA
                     VDEILAAVTA"
     gene            complement(1340..2257)
                     /locus_tag="VFP02_05535"
     CDS             complement(1340..2257)
                     /locus_tag="VFP02_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542518.1"
                     /translation="MLEGTTRDPDEVVAVVPPDLVDCTVEKVAVNAVMAGCRPEYLPV
                     VLAAVEAACTDEFNMHGLLATTYFSGPMILVNGPLARGIGMNSGGNALGQGNRANLTI
                     GRALQLVVRNVGGGRPDGVDRATLGHPGKLSFCFAEDEEGSPWEPLSVSRGFEPGVDT
                     VTLFAGEGVRGVVDQLSREADSLARSMAACLRTTAHPKLVLGFDAVLVVSPEHARVFR
                     EAGWSRPRLIDELTTLLTIDGQELVRGAGGIAEGLPEAFAEMSLPKFRGSGLLVVHAG
                     GGAGLFSAIIGGWVSGQGGSEPVTREIRP"
     gene            complement(2261..2797)
                     /locus_tag="VFP02_05540"
     CDS             complement(2261..2797)
                     /locus_tag="VFP02_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9542519.1"
                     /translation="MEERALAGGRTVADGLVAVVKRDCPTCVLVAPVLADLAARTDLV
                     VLTQDDPTFPEGVPDVVDDTDLDFSWHHDLDTVPTLLRFEAGEEAARIVGWDRAQWEA
                     FFGLTGLGPDLPAWRPGCGSLSVDPNLSDELAVRYGGSVLRSRRIELAALEDEFEALF
                     DRGWTDGLPVVPPTEARV"
     gene            complement(2828..>3106)
                     /locus_tag="VFP02_05545"
     CDS             complement(2828..>3106)
                     /locus_tag="VFP02_05545"
                     /inference="COORDINATES: protein motif:HMM:NF019044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542520.1"
                     /translation="DVFVALARVLFEAPGDGESAHLHCTDTQGEWLITFPAPSERVLI
                     REHAKGDVAFRGSAEALLLFLWNRDGGAVDTVGDAAVAGRWRELVPPM"
BASE COUNT          500 a         1099 c         1101 g          406 t
ORIGIN      
        1 ctgcgcgtcc tcggtgtcgt cgcccggccc gtactggtgc ggggccacga ccgcatcctc
       61 gacgacggac tccgccgttg cctcgacgcc accggcctcg acctggagcc gcagtcgacc
      121 tcagcgcgaa tccgagggca ggacgccggg taggtcagag ggaacccgat caaggaggca
      181 ccatgccgac ccgtgatcct ggcccttccg tgaaggaccc cgaacagtac gaagccctgc
      241 gcgacgaggg catgagcaag gagaaggcgg cccgcatctc gaacgcgtcg gccaacgagg
      301 gtcggagctc ggtcggccgc aagggcggcg aggccgagga ctacgaggac cgcaccgtgg
      361 acgagctccg cgaccgggcg gcggagatcg ggatcgaagg gcgatccgac atgaccaagg
      421 acgagctcat cgacgctctc cgcaaccact gacggaagcg aggacaccat gaccaccgtg
      481 accgacgaac tgctggcgct cgaacgcggg gcctgggagg cgctggccac cgacggtgaa
      541 gccgccaccg ccttctacga ccgcgtcctg gccgacgacg tgctcatgct gctgcccggc
      601 gacatggtca tcgatgaccg cgccaccgtc gtcagctcca tgagcggcgc cgcctggcag
      661 tcctacgagc tgacggatga acgggtcgtc cccctggggg aggactcggc cgtggtcggc
      721 taccggggca ccgccacccg cgacgacacg ccttacaggg cgctgttcaa cagcacctat
      781 gtgcgggagg acggcgactg gaagctcgtc ctccaccagc agacgcccgt ctaggccgtc
      841 accgccgcca ggatctcgtc gaccgccgcg tcggccagag cccgcatctc gtcatcggtg
      901 cggtcctgta tggggtgagg gacgaacacg cgtgccaccg ggaagcggag cgcgtcggcc
      961 tgggtttcgg ccgcctcgat gaactcggtg gaggcgatga aggccccggg gatgccccgc
     1021 cgctccagat ctgcgatgtc gtgcacactg cacgacgtac acgagcctca atcggcgagc
     1081 gcctcgatga cgacgtgaca gcgctgggcg atctcgtaac ggaggtcggc tggggccggc
     1141 ttggtgaagg tgggcttggc gaagcgctcg acggtcgccc cccgggcccg gaggtgggat
     1201 tccagctggt cgaggaagac gtcgccccgg ggcttgctga tgtccagcag gccgacggtg
     1261 aggccctcga gggtcgccgg tcggtcgagt agggggcggg gagcgggggc gctctcgccg
     1321 gtgggatcca gcaggacgct catggtcgga tctccctcgt cacgggctca ctgcccccct
     1381 ggccgctgac ccagcccccg atgatggcag agaagaggcc ggctcctccg cccgcatgga
     1441 ccaccagcag gccactgccc cggaacttgg gcaggctcat ctcggcgaag gcctcgggga
     1501 gtccttcggc gatgccgccg gcgccgcgga ccagctcctg gccgtcgatg gtcagcaggg
     1561 tcgtcagttc gtcgatcagg cgcggtcggc tccagcccgc ctcccggaac acccgggcgt
     1621 gctcgggcga gacgacgagg acggcgtcga agccgaggac cagcttggga tgagccgtgg
     1681 tgcgcaggca ggccgccatc gaccgggcca gggagtcggc ctcccgcgag agctggtcca
     1741 cgaccccgcg cacaccctcc ccggcgaaga gggtcaccgt gtcgacccct ggctcgaagc
     1801 cgcgggacac cgacaacggc tcccatggcg agccctcctc gtcctcggcg aagcagaagc
     1861 tgagcttgcc ggggtgaccg agcgtggccc ggtcgacacc gtccggacgg ccaccaccca
     1921 cgttgcggac gaccagttgg agcgcccgac cgatggtcag gttggcccgg ttgccctgcc
     1981 cgagggcatt gccgccgctg ttcatgccga tgccgcgggc cagcggcccg ttgaccagga
     2041 tcatcgggcc ggagaagtag gtcgtggcca gcaggccgtg catgttgaac tcgtcggtgc
     2101 aggcggcctc gaccgcagcc aggaccacgg gcaggtactc cggacgacag ccggccatga
     2161 ccgcattgac cgcgaccttc tcgaccgtgc agtcgaccag gtcggggggc acgaccgcga
     2221 cgacctcgtc ggggtcccgg gtggtgccct cgagcatgcg ctagacccgg gcctcggtgg
     2281 gcggcacgac gggcaggccg tcggtccaac cgcgatcgaa cagggcctcg aactcgtcct
     2341 cgagggcggc cagctcgatg cgacggctgc gcagcacgct ccccccgtag cgcacggcca
     2401 gctcgtcgct gaggttcggg tccacgctca gggagccgca ccccggccgc caggcgggga
     2461 ggtccggacc gaggccggtc aggccaaaga acgcctccca ctgcgcacgg tcccagccca
     2521 cgattctggc cgcctcctcg cccgcctcga agcggaggag cgtgggcacg gtgtccaggt
     2581 cgtggtgcca ggagaagtcc agatcggtgt catcgacgac gtcaggcact ccctcgggga
     2641 aagtcgggtc gtcctgggtc aggacgacca gatccgtgcg ggcagcgagg tcggccagga
     2701 ccggggcaac gagcacacag gtcgggcagt cccgcttcac cacggcgacg aggccgtcgg
     2761 cgaccgtgcg accgccggcc agggcccgct cctccacgct cagcgaccgt actccctgat
     2821 tcgggcgtca catcggcggg acgagctcgc gccagcgtcc ggccacggcc gcgtccccca
     2881 ccgtgtccac cgcaccgccg tcccggttcc agaggaacag caggagggcc tcggcgctcc
     2941 cccggaaagc cacgtcgccc ttggcgtgct cgcggatcag gacccgctcg ctcggcgccg
     3001 gaaaggtgat gagccactcg ccctgggtgt ccgtgcagtg caggtgggcg ctctccccgt
     3061 cacccggggc ttcgaacagg acccgggcga gggccacgaa cacgtc
//
LOCUS       DASTFV010000293         2631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1100777, whole genome shotgun sequence.
ACCESSION   DASTFV010000293 DASTFV010000000
VERSION     DASTFV010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100777"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VFP02_05550"
     CDS             <1..867
                     /locus_tag="VFP02_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET9542521.1"
                     /translation="EVNAPLLSRSTLFRLEPLGPDAVRTLIERGLVEEQATADDEAIE
                     HLVAQVGGDGRQVLTALEVAVALAAADPGDSDAEIGLAHVEAALNAKALRYGEDEHYD
                     VISAFIKSIRGSDPDAALYWLALMLESGEDARFIARRLVISASEDIGLADSMGLVVAN
                     AAAQAVEFVGLPEARIALAHATVYLAAAPKSNRSYAALGKALADVQDQPRGEVPLHLR
                     GTGYRGAASLGHGEGYDYPHDDPRGWVDQEYRPPGLAGRRYYEPSTQGAERRVAERMA
                     ERTSPPKDEESP"
     gene            864..1232
                     /locus_tag="VFP02_05555"
     CDS             864..1232
                     /locus_tag="VFP02_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542522.1"
                     /translation="MSGTEVAALVVAVAAGITLTVLIVVVIALLRTVRDLRTTVEALR
                     VESVAVVEDLHEAVRSTNAELARIDNVLGAAESVAGTMDSASKLAYLTVGTPVVKGLA
                     LATGANQAYRRFRGVRRHSR"
     gene            1229..1480
                     /locus_tag="VFP02_05560"
     CDS             1229..1480
                     /locus_tag="VFP02_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542523.1"
                     /translation="MIKRLFWLALGITVGVLTVLRTQRSLRRRADRLRPERMATDLAD
                     AIRALGVDLRKAVAEGRTAMSQREAELNEQLQARTGADT"
     gene            1520..>2631
                     /locus_tag="VFP02_05565"
     CDS             1520..>2631
                     /locus_tag="VFP02_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HET9542524.1"
                     /translation="MDANQLRKAFTGFFEERGHSVVPSASLIPHDKTVLFTVAGMVPF
                     KPYFVGDEPAPWPRVVSVQKCVRAGGKHNDLDEIGRTKRHLTFFEMLGNFSFGDYFKE
                     RAIPLAWEFVTEVLGLDPDRLWVTVHTTDDEAEALWHDVVGLPLERIQRLDEDNFWQM
                     ADTGPCGPSSEIFWDMGDEYGEAGGPAHGGAERFVEIWNLVFMQYDQHADGSRAPLPE
                     PSIDTGAGLERILTVLQDEDSIFHTDEFRRLIAKASALSGVAYGGDERTDVSLRILAD
                     HARAMTFLVNDGVIPSNEERGYVLRRIIRRAVRHAYLLGVDEVITPALVDEVVAIMAD
                     DYPELVESHAVVRQTIEREETRFRETLRTGSAILETA"
BASE COUNT          399 a          971 c          895 g          366 t
ORIGIN      
        1 gaggtcaacg ccccactcct gtcccggtcg accctgttcc gcctcgaacc actgggcccc
       61 gacgcggtgc gcaccctgat cgagcgcggc ctcgtcgagg aacaggcgac agcggacgac
      121 gaggccatcg agcacctcgt ggcccaggtc ggcggcgatg gccgtcaggt cctcaccgcc
      181 ctcgaagtgg ccgtcgccct ggccgccgcc gacccgggtg acagtgacgc tgagatcggc
      241 ttggcccacg tcgaggccgc cctcaacgcc aaggccctgc gctacgggga ggacgagcac
      301 tacgacgtca tcagcgcctt catcaagagc atccgcggct cggaccccga cgccgccctc
      361 tactggctgg ccctgatgct cgagtcgggc gaggacgccc gcttcatcgc ccgccgcctg
      421 gtcatctccg cctccgagga catcggcctg gcggattcga tgggcctggt ggtggccaac
      481 gccgcggccc aggccgtcga gttcgtgggt ctgcccgagg cccgcatcgc cctggcccac
      541 gccaccgtct acctggccgc cgcccccaag tcgaaccggt cctacgccgc cctgggcaag
      601 gctctggccg acgtccagga ccagccccgg ggcgaggtcc cgctccacct gcgcggcacc
      661 gggtaccggg gtgcggcatc cctgggccac ggcgagggct acgactaccc tcacgatgat
      721 ccccggggtt gggtcgacca ggagtaccgg ccccccggcc tcgcgggtcg gcgttactac
      781 gagccatcga cgcagggcgc cgagcgtcgt gttgccgagc ggatggccga gcgcacgagt
      841 cccccgaaag acgaggagag cccatgagcg gcactgaggt cgcggcactg gtcgtcgccg
      901 tcgctgcggg gatcaccctc accgtgctca tcgtggtggt gatcgccctc ctgcgcacgg
      961 ttcgggacct gcgcaccacc gtcgaggccc tgcgggtgga gagcgtggcg gtggtcgagg
     1021 acctgcacga ggcggtccgg agcacgaacg ccgagctggc ccgcatcgac aacgtcctcg
     1081 gggcggccga gtcggtggcc gggacgatgg actcggcctc caagctggcg tacctgaccg
     1141 tgggcacgcc cgtggtcaag ggcctggcgc tggccacggg cgccaaccag gcctaccgcc
     1201 gcttccgggg cgtccggagg cactcgcggt gatcaaacgg ctgttttggc tcgcgctcgg
     1261 gatcaccgtc ggcgtgctca ccgtgctccg cacccagcgc tccctgcggc gacgagcgga
     1321 tcgcctgcgg ccggaacgga tggcgaccga cctggccgac gccatccggg ccctgggcgt
     1381 cgacctgcgc aaggccgtgg ccgagggtcg gacggccatg agccagcgcg aggccgagct
     1441 gaacgagcag ctccaggccc gcaccggcgc cgatacctga acggtcgggt tgggccccgc
     1501 ccgtaggctg gggcggacca tggacgccaa ccagctgcgc aaggccttca ccggcttctt
     1561 cgaggagcga ggccactccg tcgtgccctc ggccagcctg atcccgcacg acaagacggt
     1621 gctcttcacc gtggccggga tggtgccctt caagccctac ttcgtgggcg atgagccggc
     1681 cccgtggccg cgcgtggtgt cggtgcagaa atgcgttcgc gccgggggca agcacaacga
     1741 cctcgacgag atcggacgga cgaagcgcca cctgaccttc ttcgagatgc tgggcaactt
     1801 cagcttcggc gactacttca aggagcgggc catccccctc gcctgggagt tcgtgaccga
     1861 agtcctcggc ctcgaccccg accgcctctg ggtcaccgtc cacaccaccg acgacgaggc
     1921 cgaagccctc tggcacgacg tcgtcggcct gcccctggaa cgcatccagc gcctggacga
     1981 ggacaacttc tggcagatgg ccgacaccgg cccctgcggg ccgagctcgg agatcttctg
     2041 ggacatgggc gacgagtacg gcgaggccgg cgggccggcc cacggcggcg cggagcgctt
     2101 cgtcgagatc tggaacctcg tcttcatgca gtacgaccag cacgccgacg gctcccgcgc
     2161 gccgctgccc gagcccagca tcgacaccgg ggcgggcctg gagcgcatcc tcaccgtgct
     2221 gcaggacgag gactccatct tccacaccga cgagttccgc cgcctgatcg ccaaggccag
     2281 cgccctcagc ggcgtggcct atggcggcga cgagcgcacc gacgtctccc tgcgcatcct
     2341 ggccgaccac gcgcgggcca tgaccttcct cgtgaacgac ggggtcatcc ccagcaacga
     2401 ggagcggggt tacgtcctcc gccgcatcat ccgccgagcg gtacgccacg cctacctgct
     2461 cggcgtggat gaggtgatca cgcccgccct ggtcgacgag gtcgttgcca tcatggccga
     2521 cgactacccc gagctggtcg agagccacgc cgtggtgcgc cagaccatcg agcgggagga
     2581 gacccgcttc cgggagacgt tgcgcacggg gtcggcgatc ctcgagaccg c
//
LOCUS       DASTFV010000294         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1103810, whole genome shotgun sequence.
ACCESSION   DASTFV010000294 DASTFV010000000
VERSION     DASTFV010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103810"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VFP02_05570"
     CDS             complement(<1..659)
                     /locus_tag="VFP02_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9542525.1"
                     /translation="MATKSSSTLSRYDWSAPVDAAARPWAQIYDHFRFYARIVRNLPQ
                     GLRYRREIVNLISDITIGAGALVVGGGMFFVIFAMSFFTGTEVGLQGFKGLEQIGAEA
                     FTGVISSVANTREITPLIAGVALAAQVGAGFTAQLGAMRISDEIDALEVMGVDSFQYL
                     VATRVWASLLTMIPLYLAALFASYLATELIVTDFFGLGSGQYRHYFNLFLPPIDIFYS
                     FV"
     gene            complement(665..1456)
                     /locus_tag="VFP02_05575"
     CDS             complement(665..1456)
                     /locus_tag="VFP02_05575"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9542526.1"
                     /translation="MSTTTLPLAGKATSLFRETGKMTAVGLEASRKVFSRPWPFAEFL
                     DQLWFLAKVTTLPVILISIPFGMVISLQVGALIKQLGAEAHLGSALVLAVVREQAPIA
                     TALIIAGAGGSAMCADLGARRIRDEISAMEVMGVNPIQRLVMPRILAATVLALLLDGV
                     TSVAGIAGGWFFGVKVQGVSSSSFFASFDELSQPWDLWMALIKAACFGFLAGTVACYK
                     GLYAKGGPKGVGDAVNQAVVITFVLLFFVNFVLTLVYFNFIPQKV"
     gene            complement(1456..2352)
                     /locus_tag="VFP02_05580"
     CDS             complement(1456..2352)
                     /locus_tag="VFP02_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9542527.1"
                     /translation="MGAATELPERPPAPAAESEAIISIKGLTKSFGAHTVLEDMSFDI
                     PRGEISAIMGPSGTGKSELLKHIIGLLKPDRGEVWIDGEEITTMRDRDLMRVRRKLGV
                     LFQDGALFGSLNLFDNIAFPLREHTSKSEKEIKRIVLDKSSLVGLLDHMKKFPGEVSG
                     GMKKRAGLARALAMDPEIVLFDEPDSGLDPVRVSYLDELVKKTQEETGATFFIITHNI
                     PSAMRTADHIGVLYQSHLVRFAAKDDMRASDDPIINQFLRGRAYGPIGMDELATEETD
                     VERELVGRAQEREQRDGEFLPV"
     gene            2574..>2771
                     /locus_tag="VFP02_05585"
     CDS             2574..>2771
                     /locus_tag="VFP02_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542528.1"
                     /translation="MPFATIGATLGLLGALTLVGTGLGDDHHENLESLSDRPDLPLGS
                     PLPGARGAEDTALDLAAAFQAV"
BASE COUNT          462 a          900 c          993 g          416 t
ORIGIN      
        1 acgaacgagt agaagatgtc gatcgggggc aggaacaggt tgaagtagtg ccggtactgg
       61 cccgacccga ggccgaagaa gtcggtgacg atcagctcgg tggccaggta gctggcgaag
      121 agggccgcca ggtagagcgg gatcatcgtc agcagcgagg cccacacccg ggtggcgacc
      181 aggtactgga agctgtcgac gcccatgacc tcgagggcgt cgatctcgtc ggagatgcgc
      241 atggccccga gctgggcggt gaagccggcg ccgacctggg cggccagggc cacgccggcg
      301 atgagggggg tgatctcccg ggtgttggcc accgaggaga tgacgccggt gaaggcctcg
      361 gccccgatct gctcgaggcc cttgaagccc tgcaagccga cctcggtgcc ggtgaagaag
      421 ctcatggcga agatgacgaa gaacatcccg ccgccgacga ccagagcgcc ggccccgatg
      481 gtgatgtcgc tgatcaggtt gacgatctcc cgccggtacc tcaggccctg gggcaggttg
      541 cgcacgatcc gggcgtagaa gcggaagtgg tcgtagatct gggcccacgg ccgggccgcg
      601 gcgtcgaccg gcgcggacca gtcgtagcgg gagagtgtgg aggaggactt cgtcgccatc
      661 gccgtcagac cttctgcggg atgaagttga agtacacgag cgtcagcacg aagttgacga
      721 agaagaggag gacgaaggtg atgaccaccg cctggttcac cgcgtcgccg acgcccttgg
      781 gcccgccctt ggcgtagagg cccttgtaac aggccaccgt cccggccagg aacccgaagc
      841 aggccgcctt gatgagggcc atccagaggt cccacggctg ggagagctcg tcgaagctgg
      901 cgaagaagct gctggaggag acgccctgga ccttcacgcc gaagaaccag ccgccggcga
      961 tgccggccac cgacgtgacg ccgtcgagca ggagggccag caccgtggcg gccaggatgc
     1021 ggggcatcac caggcgctgg atggggttca ctcccatgac ctccatggcc gagatctcgt
     1081 cgcggatgcg acgagccccg aggtcggcgc acatggccga gccaccggcg ccggcgatga
     1141 tgagggcggt ggcgatgggg gcctgctccc gcacgaccgc cagcacgagg gccgagccga
     1201 ggtgggcctc ggcgccgagc tgcttgatga gggccccgac ctgcagggag atgaccatgc
     1261 cgaacgggat ggagatgagg atcaccggca gcgtggtgac cttggccagg aaccagagct
     1321 ggtcgaggaa ctcggcgaac ggccacgggc ggctgaacac cttgcgactg gcctcgaggc
     1381 cgaccgcggt cattttcccg gtctcccgga agaggctcgt cgcctttccg gccaggggaa
     1441 gcgtcgtggt ggacatcaga ccgggaggaa ctcgccgtcg cgctgctcgc gctcctgggc
     1501 ccgcccgacc agctcgcgct cgacatcggt ctcctcggtg gccagctcgt ccatcccgat
     1561 gggcccgtac gcccggccgc ggaggaactg gttgatgatc gggtcgtccg aggcgcgcat
     1621 gtcgtccttg gccgcgaagc gcacgaggtg gctctggtag aggacgccga tgtggtcggc
     1681 ggtgcgcatg gccgacggga tgttgtgggt gatgatgaag aaggtggccc cggtctcctc
     1741 ctgggtcttc ttcaccagct cgtcgaggta ggacacgcgg acggggtcga ggccggagtc
     1801 gggctcgtcg aagaggacga tctcgggatc catggccagg gcccgggcca ggccggcccg
     1861 cttcttcatc cctccggaca cctcgccggg gaacttcttc atgtggtcga ggaggccgac
     1921 caggctggac ttgtccagca cgatcctctt gatctccttc tcggacttgc tggtgtgctc
     1981 gcgcagcggg aaggcgatgt tgtcgaacag gttcagggag ccgaagaggg ccccgtcctg
     2041 gaagaggaca ccgagcttgc gccggacccg catgaggtcg cggtcgcgca tcgtggtgat
     2101 ctcctcaccg tcgatccaga cctcgccccg atccggcttc agcaggccga tgatgtgctt
     2161 gaggagctcc gacttgccgg tccccgacgg gcccatgatg gcgctgatct cgccccgggg
     2221 gatgtcgaag gacatgtcct cgagcaccgt gtgggcgccg aaggacttgg tcagtccctt
     2281 gatggagatg atcgcctcgg actcggctgc cggcgcagga ggccgttcgg ggagttcggt
     2341 tgccgctccc atgtcccact gcttccgtcc aacgtgcgta cacgggccaa gaccgcgtga
     2401 ccggggtcac gtgacgccgg tgcgccgagc gatcagcgcc ggccgccacc ctcgacctcg
     2461 gatcgacccc ccctcgaccg agcatcgagg gtctggcggg ggtctcaggc cgaaaatccc
     2521 ggtgtccggc gtcacgcgac accccgggcc cggtctccat cgacatgcac gccatgccgt
     2581 tcgccaccat cggcgccacc ctgggcctgc tcggggccct gaccctggtc ggcacggggc
     2641 tgggcgacga ccaccacgag aacctcgaga gcctgtcgga ccgccccgat ctccccctcg
     2701 gctccccgct cccgggcgca aggggggccg aggacacggc gctcgacctg gccgccgcct
     2761 tccaggccgt c
//
LOCUS       DASTFV010000295         4215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1108789, whole genome shotgun sequence.
ACCESSION   DASTFV010000295 DASTFV010000000
VERSION     DASTFV010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108789"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VFP02_05590"
     CDS             <1..1052
                     /locus_tag="VFP02_05590"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET9542529.1"
                     /translation="RQYASLKFFLYTMFGSALMLVAFLALFFVTGANSDPNGSFAFSW
                     FLEQAGSLDRDVQVWIFAGFFVGFAVKVPMFPFHTWLPDAHTEAPTQGSVILAAILLK
                     LGTYGFMRIAIPFLPDGAEAWMPAVGILAVIGIIYGALCCLAQTDMKRLIAFSSVAHM
                     GFTMLGISTLTDFGMNAAVFGMVAHGLITGMLFFLAGSVKHRYHTLEIGRLSGMLVSM
                     PRLGWILGFCAMASLGLPGLAGFWGEFLAILSAYSPAPGLPVETFRTYMVIAAVGTVL
                     AAGYLLWLYQRTTFGVPKEEFADEEIAQLSIFEWVAWIPMLVPILVLGIYPNLIFSVT
                     DPAVEKVLHSLTGGG"
     gene            1054..2559
                     /locus_tag="VFP02_05595"
     CDS             1054..2559
                     /locus_tag="VFP02_05595"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET9542530.1"
                     /translation="MIALLAQAGGFTAPTIDYHAFAPEIVLTGVLVVVLLADLVFERD
                     KWLIPSLTGIGVLAALVPVLTLALDGTDRVMFDGAYVVDDFALVMKALFLIAGYVVIL
                     LSTNYIAEGDYHEGEYYFLLLSSLLGMSVMASARDLISIFVALELLSIPAYMLAAWRK
                     RDLKGNEAGLKYYLMGVFASAVLLYGMSLLYGVTGSTLLTEIGAQIGAKPSSVPFITL
                     GILFIIVGFGFKVSAVPFHTWAPDTYEGAPTPIASFLAVASKAAGFVALLSLVFLAFP
                     GRSDVVEPLMWILAAVTMTVGNLIALRQTNIVRLLAYSSVAQAGFMLAPLAVVGDAGQ
                     EALTAVVVYLLIYTAMNLGAFAVVIAVARRTGSGEIDSYRGLFQYSPALAVLMSVFLF
                     ALAGIPPLAGWVAKFVVFRALLSAETVSGAVLAVVVAVNSVIALFYYANVARLMWFQD
                     APEGETTSIRVPFSLSAALGIAVIGTLVFGIFPGLVTHFGDAANLAASAIP"
     gene            2583..3479
                     /locus_tag="VFP02_05600"
     CDS             2583..3479
                     /locus_tag="VFP02_05600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET9542531.1"
                     /translation="MEAALYGPEGFYEREGGGAGRRGDFLTAPEVGPLFGAVLVRALD
                     AWWDAQGRPAAFTVYDVGAGAGTLGRSLALAGSPHRYVAVDRSRAQRARHQGLESAAD
                     LPDGPLVGVVVANELLDNLPFDLTVGDQPFDPGQDWPETGLAPRQDQARQWVEGVLAR
                     LRGRLVVVDYVSTTAAMATRPWPEWLRTYRGHDRGGHPMDHLGGQDITAEVAVDQLPA
                     PDEDHSQADWLRSWGIDDLVEEGRRIWQERAHLGDLEAIRARSRVNEAEALLDPAGMG
                     SFRVLEWSVVDEPGEGNPRGAV"
     gene            3519..3698
                     /locus_tag="VFP02_05605"
     CDS             3519..3698
                     /locus_tag="VFP02_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542532.1"
                     /translation="MIRALIVGLVVFLIATFPATWLLMLFLGNLGLGLSYWGALPLGI
                     LVSALIGGSSANTYA"
     gene            3765..>4215
                     /locus_tag="VFP02_05610"
     CDS             3765..>4215
                     /locus_tag="VFP02_05610"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HET9542533.1"
                     /translation="MTEAPAPRILSPTVLESVTELDGAVDALRALVDDALPPGDVRDA
                     LQGEWLGHPVHPVLTDLAIGFWTSAFVLDLVPAKRLRAAADVFVALGLAAALPTVVTG
                     LADWSRLPRERQRVGVVHAGSNAVASGFYAWSLAARVRGRRARGIALG"
BASE COUNT          515 a         1540 c         1404 g          756 t
ORIGIN      
        1 agcggcagta cgcatcgctc aagttcttcc tctacaccat gttcggttcg gcgctcatgc
       61 tcgtggcgtt cctcgcgctc ttcttcgtca ccggtgccaa cagcgacccg aacggcagct
      121 tcgcgttctc ctggttcctc gagcaggccg gctcgctcga ccgcgacgtc caagtgtgga
      181 tcttcgccgg gttcttcgtc gggttcgccg tgaaggtgcc gatgttcccg ttccacacgt
      241 ggctgcccga cgcccatacc gaggcaccga cgcaggggtc ggtgatcctg gcggcgatcc
      301 tgctgaagct cggcacgtac ggcttcatgc gcatcgccat cccgttcctt cccgacggcg
      361 ccgaggcgtg gatgcccgcc gtcggcatcc tcgccgtgat cggcatcatc tacggcgcac
      421 tctgctgcct ggcccagacc gacatgaagc gcctgatcgc cttctcgtcg gtggcccata
      481 tgggcttcac gatgctcggc atctcgacgc tcaccgattt cgggatgaac gccgccgtct
      541 tcggaatggt cgcccatggc ctgatcaccg ggatgctgtt cttcctcgcc ggttcggtga
      601 agcaccgcta ccacaccctc gagatcgggc gcctgtccgg gatgctcgtg tcgatgccgc
      661 gactcgggtg gatcctcggc ttctgcgcca tggccagcct cgggctgccc ggcctggccg
      721 ggttctgggg cgagttcttg gcgatcctgt cggcatacag cccggcgccg ggcctgccgg
      781 tggagacgtt ccggacctat atggtcatcg ccgcggtcgg cactgtcctg gccgcgggct
      841 acctgctgtg gctgtaccag cggaccacct tcggggtccc caaggaggag ttcgccgacg
      901 aggagatcgc ccagctctcg atcttcgaat gggtggcctg gatcccgatg ctggtgccga
      961 tcctcgtcct gggcatctac cccaacctga tcttcagcgt caccgatccc gccgtggaga
     1021 aggtgctcca ctccctgacc ggcggcggct gacgtgatcg ccctgctcgc ccaggccggg
     1081 gggttcaccg ccccgacgat cgattaccac gccttcgcgc ccgagatcgt gctcaccggc
     1141 gtcctggtcg tcgtgctcct ggccgacctc gtgttcgagc gggacaagtg gctgatcccg
     1201 tcgctgacgg gcatcggcgt gctggccgcg ctggtccccg tcctcaccct cgccctcgac
     1261 ggcaccgacc gggtgatgtt cgacggggcc tacgtcgtcg acgacttcgc cctggtcatg
     1321 aaggccctgt tcctcatcgc gggctacgtg gtcatcctgc tgtcgaccaa ctacatcgcc
     1381 gagggcgact accacgaggg ggagtactac ttcctgctcc tcagctcgtt gctgggcatg
     1441 tcggtcatgg cctcggcccg ggacctgatc tccatcttcg tggccctgga gctgctctcg
     1501 atccccgcct acatgctcgc cgcctggcgc aagcgggacc tcaagggcaa cgaggcgggc
     1561 ctgaagtact acctgatggg cgtcttcgcc tcggcggtgc tcctctacgg catgtccctg
     1621 ctctacgggg tcaccggttc gaccctgctc accgagatcg gggcccagat cggggccaag
     1681 ccgtccagcg tgccgttcat cacgctgggg atcctgttca tcatcgtggg cttcggcttc
     1741 aaggtctccg ccgtcccgtt ccacacctgg gcccccgaca cctacgaggg ggccccgacg
     1801 cccatcgcct cgttcctggc cgtggcgtcc aaggcggcgg gcttcgtggc cctgctgtcc
     1861 ctggtcttcc tggccttccc cggccggagc gacgtcgtcg agccgctcat gtggatcctg
     1921 gccgcggtga ccatgaccgt cggcaacctg atcgccctgc gccagaccaa catcgtgcgc
     1981 ctcctggcgt actcctcggt ggcccaggcc ggcttcatgc tcgccccgct ggccgtcgtc
     2041 ggggacgccg gccaggaggc cctgaccgcg gtcgtcgtct acctgctcat ctacacggcc
     2101 atgaacctcg gcgccttcgc cgtggtgatc gcggtggccc gacgcaccgg atcgggcgag
     2161 atcgactcgt accggggcct gttccagtac tcgcccgccc tcgcggtgct gatgtcggtg
     2221 ttcctcttcg ccctggccgg catcccgccg ctcgccggtt gggtggcgaa gttcgtcgtc
     2281 ttccgagccc tgctctccgc cgagaccgtg tccggtgccg tgctggccgt ggtcgtcgcc
     2341 gtgaactcgg tgatcgcgct cttctactac gccaacgtgg cccgcctgat gtggttccag
     2401 gacgcccccg agggcgagac cacgtccatc cgggtgccgt tctcgctcag cgccgccctc
     2461 ggcatcgccg tcatcggcac gctggtcttc ggcatcttcc ccggcctggt cacccacttc
     2521 ggcgacgcgg ccaacctcgc cgccagcgcg atcccctgac cgcgcctccg ttctcggagg
     2581 tcatggaggc cgcgctctac gggccggagg ggttctacga gcgggaaggg ggaggcgccg
     2641 ggcggcgcgg cgacttcctc accgcccccg aggtcgggcc cctcttcggg gccgtgctcg
     2701 tccgcgcgct ggacgcctgg tgggatgcgc agggtcgacc ggccgccttc accgtgtacg
     2761 acgtcggggc cggggcgggc acgctgggcc gcagcctggc gctggccggc agcccccacc
     2821 gctacgtggc cgtcgaccgt tcgagggccc agcgggcccg gcaccagggc ctcgagtcgg
     2881 cggccgatct gcccgacggc ccgctcgtcg gcgtggtcgt cgccaacgag ctgctggaca
     2941 acctgccgtt cgacctgacg gtcggggacc agcccttcga tcccggacag gactggccgg
     3001 agacgggcct cgccccgcgg caggatcagg ctcgccaatg ggtggagggc gtcctggccc
     3061 ggctgcgcgg ccgcctggtc gtcgtcgact acgtctcgac gacggccgcc atggctaccc
     3121 gcccctggcc cgagtggttg cgcacctatc ggggtcatga ccggggcggg cacccgatgg
     3181 accacttagg cggccaggac atcaccgccg aggtggccgt cgaccagctc ccggcgcccg
     3241 acgaggacca cagccaggcc gactggctcc ggtcgtgggg catcgacgac ctggtggagg
     3301 agggtcggcg gatctggcag gagcgggccc acctcggcga cctggaggcc atccgggccc
     3361 gcagtcgggt caacgaggcc gaggccctgc tggaccccgc cggcatgggc tcgttccggg
     3421 tcctcgagtg gtctgtggtc gacgagcccg gagaagggaa tccccgaggt gcggtctgac
     3481 atgtgccaca ctcggcgccg gaacgagggg gattctccat gattcgtgca ctcatcgtcg
     3541 ggctggtcgt gttcctgatc gccaccttcc cggccacctg gttgctgatg ctgttcctgg
     3601 gcaacctggg cctcgggctg tcctactggg gcgcgctgcc cctcgggatc ctggtctccg
     3661 ccctcatcgg cggaagcagc gcgaacacct acgcctgacc gccgaacgcg ggtgacccac
     3721 gccgggtggg aacgaggacc gcgttgaccg ggtaggaggc tgggatgacc gaggccccag
     3781 caccacgcat cctctcgccg accgtcctgg agagcgtgac tgagctcgac ggcgcggtcg
     3841 acgcgctgcg cgccctggtg gacgacgccc tcccaccggg cgacgtgcgc gacgccctcc
     3901 agggcgagtg gctcggccac cccgtccacc ctgtgctcac cgacttggcc atcgggttct
     3961 ggacgagcgc cttcgtgctc gacctggtcc cggcgaagcg cctgcgagcc gcggccgacg
     4021 tcttcgtcgc cctcggcttg gcggcggccc tgccgacggt cgtcaccggc ctggccgact
     4081 ggagccggct gccccgggaa cggcagcgcg tgggcgtcgt gcacgccggg agcaacgccg
     4141 ttgcctccgg cttctacgcc tggtcgctgg cggcccgcgt tcggggccgg cgggcccgag
     4201 gcatcgccct cggga
//
LOCUS       DASTFV010000296         3696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1113304, whole genome shotgun sequence.
ACCESSION   DASTFV010000296 DASTFV010000000
VERSION     DASTFV010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113304"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFP02_05615"
     CDS             complement(<1..477)
                     /locus_tag="VFP02_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542534.1"
                     /translation="MRGGGGSFRRYRFQADLRRRAGAWLGVAVIIGLSAGLALALVAG
                     ARRSDSAVQRFVDTPAAHQSLVVNGIPGTFDFASVDFDEVAALPGVAHTQALSVLAAA
                     GRTEGDALIDTPSVNFMADASGRMGRDISPFKYLAGRPADPADPHEVVAAFRTAEAF"
     gene            complement(544..1872)
                     /gene="glmM"
                     /locus_tag="VFP02_05620"
     CDS             complement(544..1872)
                     /gene="glmM"
                     /locus_tag="VFP02_05620"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET9542535.1"
                     /translation="MTLRFGTDGVRGHADTELTPELVTALGRAAARVLGAGPVVIGRD
                     TRASGPRIEADLAAGLVAEGLTVDNVGVVPTPAVAHASMTRSVTGAMISASHNAYGDN
                     GIKFFAAGGRKLSDEVEERLEAELDRLLHGPVPVPPPVAAPEDDSVGATWVDAVVASV
                     PAGALDGLHLVVDCANGSASHWAPGALERLGARVEVIHAGPDGTNINAGCGSTHPEDL
                     QARVRALGADAGLAFDGDADRVLGVDATGALVDGDHLIAICAVDRHRRGELVDDTVVV
                     TVMANLGFRLAMEANGIRVEETAVGDRYVLEALARGGWSLGGEQSGHVIFPGLATTGD
                     GLLTGVQVLAAVQAAGQTLAEAATVMTSLPQVLLNVAVAERRPDIADRLAGPIAEAEA
                     RLGARGRVLIRPSGTEPLVRVMVEAEDTGLADEVAGTLAAAVDVASRAVG"
     gene            complement(1886..2278)
                     /gene="rpsI"
                     /locus_tag="VFP02_05625"
     CDS             complement(1886..2278)
                     /gene="rpsI"
                     /locus_tag="VFP02_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET9542536.1"
                     /translation="MPKPLVQATGRRKRSIARVRVHPGTGVVTVNQKPVADFFPSESQ
                     VMHATEPLRTVTVEESYDIDATIHGGGFSGQSGALRLGIARGLIELHPDERATLKAEG
                     MLTRDPRKKESKKYGLKKARKAPQYSKR"
     gene            complement(2308..2763)
                     /gene="rplM"
                     /locus_tag="VFP02_05630"
     CDS             complement(2308..2763)
                     /gene="rplM"
                     /locus_tag="VFP02_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET9542537.1"
                     /translation="MPTYTPKASEIQRSWHLVDADGLVLGRMATEVARILRGKHKPSF
                     TPNLDTGDHVIVVNAAKIVLTAGKAERKIIYRHSGYPGGLKSRTYADALERDAAEAVR
                     QSIVGMLPKNRLGRQMATKLKVYAGPTHPHGAQDPTPLELAHARARTND"
     gene            complement(2882..3442)
                     /locus_tag="VFP02_05635"
     CDS             complement(2882..3442)
                     /locus_tag="VFP02_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HET9542538.1"
                     /translation="MESLLAALGLVFIAELGDKTQLVALGFGARYRLAPVLAGVVAAY
                     ALTNLLSVVVGGLLGAAFPTRAIGLVGGGLFIGFALWTLRSGDDGEEDGAAAADDRSV
                     FLSVAVAMFVAELGDKTMLATATLAAQGDPVMVWIGATIGIILAGTLGVLLGRFFGAR
                     LPERVTRIGSAVLFAGFGVVLILTNL"
     gene            complement(3442..>3696)
                     /locus_tag="VFP02_05640"
     CDS             complement(3442..>3696)
                     /locus_tag="VFP02_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542539.1"
                     /translation="DPASLTRRVVDARWAELDDDLLRFEVTANAFCHQMVRSIVGLLV
                     DVGRGRSRAGDILGLIRAQDRTNIPAPAPPQGLCLWSVAY"
BASE COUNT          519 a         1264 c         1433 g          480 t
ORIGIN      
        1 gaaggcctcg gcggtccgga aggcggcgac cacctcgtgg ggatcggcgg gatcggccgg
       61 gcggccggcg aggtacttga agggtgagat gtcccggccc atccgccccg atgcatcggc
      121 catgaagttg acggacgggg tgtcgatgag cgcgtcgccc tcggtccgcc ccgccgccgc
      181 gagcaccgac agcgcttggg tgtgggccac gccggggagc gcggcaacct catcgaagtc
      241 gaccgaggcg aagtcgaagg tcccggggat gccgttgacc accagcgact ggtgggcggc
      301 gggcgtgtcg acgaagcgtt ggacggcgga gtcggaacgg cgggctccgg ccacgagggc
      361 tagggccaac ccggccgaca ggccgatgat gaccgcgacg cccagccagg caccggcgcg
      421 gcgacggaga tcggcctgga accggtaccg cctgaaggac cctcccccac cccgcatcag
      481 cccaagtgtc gcccagcgtc ggcgcgggtg gcgaggaggc gggccttcat ccggcggggg
      541 tggctacccc accgcccgag aagcgacgtc gacggcggcg gccagggtgc cggcgacctc
      601 gtcggcgagc ccagtgtcct cggcctcgac catgacccgg acgaggggtt cggtcccgct
      661 gggccggatc aggacccggc cccgggcgcc caaccgggct tcggcctcgg cgatgggccc
      721 ggccaggcgg tcggcgatgt cggggcggcg ctcggccacg gccacgttga gcaggacctg
      781 cggcagcgag gtcatgacgg tggccgcctc ggccagggtc tggccggcag cctgcacggc
      841 ggccaggacc tggaccccgg tgagcaggcc gtccccggtg gtggccagcc cggggaagat
      901 gacgtgcccg gactgctcgc ccccgagcga ccagccgccg cgggccaacg cttcgagcac
      961 gtaccggtcg cccaccgccg tctcctcgac ccggatcccg ttggcctcca tggccaggcg
     1021 gaagccgagg ttggccatca ccgtcacaac gacggtgtcg tcgaccagct cgccccgccg
     1081 gtgacggtcc acggcgcaga tggcgatgag gtgatcgccg tcgaccagcg cgccggtggc
     1141 atcgacgccc agcacccggt cggcgtcccc gtcgaaggcc agtccggcgt cggctccgag
     1201 agcccggacc cgggcctgca ggtcctcggg gtgggtcgat ccgcagccgg cgttgatgtt
     1261 ggtgccgtcg ggcccggcgt ggatgacctc gaccctggcc ccgaggcgtt cgagggcgcc
     1321 cggggcccag tgcgacgccg agccgttggc gcagtccacg acgaggtgca ggccatcgag
     1381 cgcgccggcg ggaaccgacg ccacgacggc gtcgacccag gtggcgccga ccgagtcgtc
     1441 ctcgggggcg gcgacgggcg gtggcaccgg taccgggcca tggaggaggc ggtccagctc
     1501 ggcctccagc cgttcctcga cctcgtcgct cagcttccgc ccgccggcgg cgaagaactt
     1561 gatgccgttg tcgccgtagg cgttgtgcga agccgagatc atggccccgg tcaccgaccg
     1621 ggtcatcgac gcgtgggcca cggccggggt ggggacgacg ccgacgttgt cgacggtcaa
     1681 cccttcggcg accaggccgg cggcgaggtc ggcctcgatg cgaggcccgg aggcccgcgt
     1741 gtcccgaccg atcacgaccg gaccggcgcc caggacccgc gccgcggcgc gcccgagggc
     1801 ggtgaccagt tccggggtca gttcggtgtc ggcgtggccc cggacaccgt cggtgccgaa
     1861 gcgaagcgtc accgggaggc cgcgactacc gcttggagta ctgcggagcc tttcgggcct
     1921 tcttcaggcc gtacttcttg gactccttct tccgcgggtc gcgggtgagc atgccctcgg
     1981 ccttgagcgt ggcccgctcg tcggggtgca gctcgatgag gccccgggcg atgcccaggc
     2041 gcagggcgcc ggactggccg ctgaagccgc caccgtggat ggtggcgtcg atgtcgtagc
     2101 tctcctcgac ggtcaccgtt cgcagcggct cggtggcgtg catcacctgg ctctcggacg
     2161 ggaagaagtc ggccacgggc ttctggttga cggtcaccac gccggtgccg ggatgcacgc
     2221 ggacacgggc gatggaccgc ttgcggcggc cggtggcctg gacgaggggc ttgggcatcg
     2281 gtacggggat ccttctcggt gaagcgttca gtcgttggtg cgggcccggg cgtgggccag
     2341 ctccagcggg gtggggtcct gggcgccgtg ggggtgggtc ggcccggcgt agaccttgag
     2401 cttggtcgcc atctggcggc ccaggcggtt cttggggagc atgccgacga tcgactggcg
     2461 cacggcctcg gccgcgtccc gctcgagggc gtcggcgtag gtccgggact tcaggccacc
     2521 gggatagccc gagtggcggt agatgatctt gcgctcggcc ttgcccgcgg tgaggacgat
     2581 cttggcggcg ttgaccacga tcacgtggtc gccggtgtcc aggttcgggg tgaacgacgg
     2641 cttgtgcttg ccccgcagga tgcgggccac ctcggtggcc atccggccga ggacgaggcc
     2701 gtcggcgtcg acgaggtgcc aggaccgctg gatctcgctg gctttggggg tgtacgtggg
     2761 cacgggctga cctgtcgtcg aacgtcggga ttcgggcgcg gcgggcgaag cgaggcccac
     2821 gggagcgcct gacaacgata cgagcgcccc ggtgcggcgg caacccggcc caggggggag
     2881 ctcagaggtt ggtgaggatg agcacgacgc cgaacccggc gaacagcacg gcggagccga
     2941 tgcgggtgac gcgttcgggc aggcgggcgc cgaagaaccg gcccagcagg acgccgagcg
     3001 tcccggccag gatgatgccg atcgtggccc cgatccagac catgaccggg tcgccctgcg
     3061 cggccagggt ggccgtggcc agcatcgtct tgtcccccaa ctcggccacg aacatggcca
     3121 ccgccaccga aaggaagacc gatcggtcgt cggcggcggc cgcaccgtcc tcctccccgt
     3181 cgtcccccga gcgcagtgtc cacagggcga agccgatgaa cagcccgccg ccgaccaggc
     3241 cgatggcccg ggtggggaag gcggccccga gcaggccgcc gacgaccacc gagagcaggt
     3301 tggtcagcgc gtaggccgcg accacgccgg ccaggaccgg ggccaggcgg taccgggccc
     3361 cgaagccgag ggccaccagc tgggtcttgt caccgagctc ggcgatgaac accaggccga
     3421 gcgcggccag cagggattcc atcagtaggc gacggaccag aggcagagac cctggggcgg
     3481 ggccggcgcg gggatgttcg tgcggtcctg ggcccggatg aggccgagga tgtcgccggc
     3541 tcggctccgg ccccggccca cgtcgaccag gaggccgacg atggagcgca ccatctggtg
     3601 gcagaaggcg ttggccgtga cctcgaagcg caggaggtcg tcgtcgagct ccgcccagcg
     3661 ggcatcgacc acccgccggg tcagcgacgc ggggtc
//
LOCUS       DASTFV010000297         4158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1116583, whole genome shotgun sequence.
ACCESSION   DASTFV010000297 DASTFV010000000
VERSION     DASTFV010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116583"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VFP02_05645"
     CDS             <1..765
                     /locus_tag="VFP02_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542540.1"
                     /translation="IATQAEAAYEERVALGRERGRELIAEAEIKRAEVLRQLARTRQL
                     AQLNLEQLRVGRERLLTAYEVARATIDQATGELDKSLPEAKALAERLTARVGDDETRS
                     LQELEAEFALERLEDLRILADDDPGARVSPRPKADRYPPPDAEAASEAEEVSASMAES
                     IPTEDRPSPKVFDMEAAEAEAQAEAEVVVPATDGLDGDDEGDADDADDADDADENVLD
                     LTDEAGDADENVLDLADEAATAETAEPAEDPAPTSR"
     gene            complement(784..1143)
                     /locus_tag="VFP02_05650"
     CDS             complement(784..1143)
                     /locus_tag="VFP02_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9542541.1"
                     /translation="MTFKRLVDVVQYLVLAMAVVFVIALFTNDGSSPSPTTAGTGGAV
                     AGDAVFASNCAGCHGADGTGGVGPALADGAVVEAFPDVADQVVVITEGRNGMPAFGER
                     LTAEEIQAVTDYTRDDL"
     gene            complement(1145..1711)
                     /locus_tag="VFP02_05655"
     CDS             complement(1145..1711)
                     /locus_tag="VFP02_05655"
                     /inference="COORDINATES: protein motif:HMM:NF039480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6529 family protein"
                     /protein_id="HET9542542.1"
                     /translation="MTAATPTPPSATASRLVAPVLIGAALSVTLGVYASAHDPTLEKP
                     YQLWFTDTINLKVWFATAALLLIAVQLVTALRMYGKLSIPKDLPPWFGDLHRLSGTLA
                     FAISLPVAYHCLWSLGFQTDDTRSLVHSLLGCLFYGAFATKVLVVRANGMPGWALPVV
                     GGVVLTTMVALWLTSALWFFTTVDFPGL"
     gene            1839..3911
                     /locus_tag="VFP02_05660"
     CDS             1839..3911
                     /locus_tag="VFP02_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET9542543.1"
                     /translation="MRIALRLLVVLALGAGGLALATVAVAPQVGEFFTAGSVVPPEVN
                     LRPLAERSVAYADNGSVLATFHAAENRQEVRLRRIPRTVRRTILAVEDEDFFQHNGFN
                     LRATFRALFENVSAGGIAQGGSTITQQLVKLSLLSPRQDLNRKAREAILAIELEKTMS
                     KQEILERYLNQVYFGGGAYGVQAAAEIYWGQNVEDLDWGQAALLASLIQNPVGYDPVR
                     EPDVARQRRRVALDRIVELGYITQEEADYYDVAGLPQARIDVLPEPNDYFVEEVKQQL
                     LDMEELGATPTERYNALFKGGLRIETTFSPEAQFLALSARNNVLPDTGGQFTAAMVAV
                     TPQTGAVRAMVGGPGFDRYRYNLATQGARQPGSSFKIFVLTAALEGGLVLDDTLNGSG
                     PCSFPNPGGYPDPYVANNFAGESGGTNTITGNVQASSNCGFLRLGQFVGLQNVVDVAR
                     RMGITTELDARNLSLPIGSEEVHPIDMAGAAAVLANGGIRHRPYFIDRVTDRRGNVIY
                     EHQSPGRQVVSQQSACLATEALEANVQAGTGTAAQLSNMAVAGKTGTAQSFGDAWFVG
                     YTPFLATAVWMGNAEARVPMTNVGGRTVTGGSYPAEIWHAFNEPAHNGLAYTDFPTCE
                     PTRPGRDLARYFDPEPEEPTTTTVDPATPTVTCPAGYLPGDLDGDGVVDSCVAVDTPT
                     TAPAPSTP"
     gene            3908..>4158
                     /locus_tag="VFP02_05665"
     CDS             3908..>4158
                     /locus_tag="VFP02_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542544.1"
                     /translation="MSDPTEADGDPVVALSPLELLLAVQDQDTASDQLVHRRSHLPER
                     QAVDDHAAVTAAVDAERATRETRREELARNIKRLQDEVAS"
BASE COUNT          687 a         1493 c         1444 g          534 t
ORIGIN      
        1 atcgccaccc aggccgaggc ggcctacgag gagcgagtgg ccctcggccg ggagcggggt
       61 cgagagctca tcgccgaggc ggagatcaag cgggccgagg tcctgcgcca gctggcccgc
      121 acccgccagc tggcccagct caacctcgaa caactccggg tcggtcgcga gcgcctgctg
      181 acggcctatg aggtggccag ggccacgatc gaccaggcca cgggcgagct ggacaagtcc
      241 ctacccgagg ccaaagcgct ggccgagcgc ttgaccgccc gggtgggtga cgacgagacc
      301 cgatccctgc aggagctcga ggccgagttc gccctcgagc gtctcgagga cctcaggatc
      361 ctggccgacg acgaccctgg ggcccgggtc tcgccgcgac cgaaggccga ccggtacccg
      421 ccgcctgatg ccgaagcggc gagcgaggct gaggaggtct cggcgtccat ggcggagtcg
      481 atcccgaccg aggaccgacc gtcccccaag gtgttcgaca tggaggccgc cgaagccgag
      541 gcccaggccg aggccgaggt cgtcgtaccg gccacggacg gcctggacgg tgacgacgag
      601 ggtgacgctg atgacgctga cgacgctgac gacgctgacg agaacgtcct cgatctcacc
      661 gacgaggcgg gtgacgcgga cgagaacgtc ctcgacctcg ccgatgaggc ggcgacggcc
      721 gaaacggccg agccggccga ggatcccgcg ccgaccagtc gctgaaggcc gagggggctc
      781 ggctcagagg tcgtcgcggg tgtaatccgt gaccgcttgg atctcctcgg ccgtgagccg
      841 ttcgccgaag gcgggcatgc cgttgcgtcc ttcggtgatc accacgacct gatcggcgac
      901 gtccggaaac gcctccacca cggccccgtc ggccagcgcc ggcccgacgc cgcctgtgcc
      961 gtcggcgcca tggcagccgg cgcagttgga cgcgaacacc gcgtcgcccg ctacggcgcc
     1021 acccgtcccc gcggtcgtcg gcgacgggga cgatccgtcg ttggtgaaca gggcgatcac
     1081 gaacaccacc gccatggcca ggaccaggta ctggacgacg tcgacgaggc gcttgaaggt
     1141 cacctcagag ccccgggaag tcgacggtcg tgaagaacca gagggcgctg gtcagccaga
     1201 gcgcgaccat cgtcgtgagc acgacgcccc cgacgaccgg cagggcccag ccgggcatgc
     1261 cgttggctcg gacgacgagg accttcgtgg cgaaggcgcc gtagaacagg cagccgagga
     1321 gtgagtgcac gaggctgcgg gtgtcatcgg tctggaaccc gagggaccag aggcagtggt
     1381 aggcgaccgg cagggagatc gcgaaggcca aggtgcccga gagccggtgc aggtccccga
     1441 accagggcgg gaggtccttg gggatcgaca gcttgccgta catccggagt gcggtcacga
     1501 gttggacagc gatcagcagc agggcggcgg tggcgaacca gaccttgagg ttgatggtgt
     1561 cggtgaacca cagctggtac ggcttctcca gcgtcgggtc gtgggccgag gcgtagaccc
     1621 cgagagtcac ggacagcgcg gcgccgatca acacgggggc cacgagccgt gaggccgtcg
     1681 ccgacggcgg cgtgggcgtc gcggcggtca tcagcctccc cgttgccctc gaaccaccgg
     1741 acccaccccc tggcttcggt gcaggaagcg tagttgcccc gtgtcgggaa ccccgtcccg
     1801 ccacggatcg gcgggaggcc tccggtacat ttcgagtctt gcggatcgca ctccggctcc
     1861 tcgtggtgct cgcgctcggt gccggcggac tggctctggc cacggtggcc gtcgcgcccc
     1921 aggtgggtga gttcttcacc gcgggctccg tcgtgcctcc cgaggtgaac ctccgacctc
     1981 tggccgagcg ctccgtggcc tacgcggaca acggctcggt gctggccacc ttccacgccg
     2041 cggagaaccg tcaggaggtg cggctgcgcc gcatcccccg gaccgtgcgg cgcacgatcc
     2101 tggccgtcga agacgaggac ttcttccagc acaacgggtt caacctccgg gccaccttcc
     2161 gggccctgtt cgagaacgtc agcgcggggg gcatcgccca ggggggctcc acgatcaccc
     2221 agcagctggt gaagctctcg ctgctctcgc cccgccagga cctgaaccgc aaggcccggg
     2281 aggcgatcct ggccatcgag ctcgagaaga ccatgagcaa gcaggagatc ctcgagcgct
     2341 acctgaacca ggtgtacttc gggggcggcg cctacggggt ccaggccgcc gccgagatct
     2401 actggggcca gaacgtggag gacctcgact ggggccaggc cgcgctgctg gccagcctca
     2461 tccagaaccc ggtcggctac gaccccgtgc gcgagccgga cgtggcccgt cagcgccggc
     2521 gggtcgccct ggaccggatc gtggagctgg gctacatcac ccaggaggag gccgactact
     2581 acgacgtcgc cggcctcccc caagcccgca tcgacgtcct ccccgagccc aacgactact
     2641 tcgtcgagga ggtcaagcag cagctcctgg acatggagga gctcggggcc acccccacgg
     2701 agcgctacaa cgccctgttc aaggggggcc tgcgcatcga gaccacgttc agcccggagg
     2761 cccagttcct ggccctctcg gcgcggaaca acgtcctgcc cgacaccggc gggcagttca
     2821 cggccgcgat ggtggcggtg acgccgcaga cgggggccgt ccgggcgatg gtgggcgggc
     2881 ccggcttcga ccggtaccgg tacaacctgg ccacgcaggg cgcccgacag ccggggtcgt
     2941 cgttcaagat cttcgtgctc accgccgccc tcgagggcgg gctcgtcctc gacgacaccc
     3001 tcaacggcag cggaccgtgc tcgttcccca acccgggcgg ctaccccgac ccgtacgtgg
     3061 ccaacaactt cgcgggggag agcggtggca ccaacacgat caccggcaac gtccaggcgt
     3121 cgtccaactg cggcttcctg cgcctcggcc agttcgtcgg gctgcagaac gtcgtcgacg
     3181 tggcccggcg catggggatc accaccgagc tcgacgcccg caacctctcc ctgccgatcg
     3241 gctccgagga ggtgcatccc atcgacatgg ccggcgccgc cgcggtcctg gccaacgggg
     3301 gcatccggca ccggccctac ttcatcgacc gggtgaccga ccgccgaggc aacgtcatct
     3361 acgagcacca gtcgccgggc cgccaggtgg tgtcccagca gagtgcctgc ctggccaccg
     3421 aagcgctcga ggccaatgtc caggcgggga cgggtaccgc cgcccagctc tcgaacatgg
     3481 ccgtcgccgg caagacgggc accgcccaga gcttcggtga tgcctggttc gtcggctaca
     3541 cgccgttcct ggccacggcg gtctggatgg gcaacgccga ggcccgggta ccgatgacga
     3601 atgtgggcgg ccggaccgtg accggcggct cgtacccagc cgagatctgg cacgcgttca
     3661 acgagcctgc ccacaatggg ctggcgtaca ccgacttccc gacctgcgaa ccgacccgcc
     3721 cgggacggga cctggcccgc tacttcgatc ccgaaccgga ggaacccacg acgaccacag
     3781 tcgaccccgc caccccgacg gtgacctgtc ccgccggcta cctgcccggc gatctcgacg
     3841 gcgacggcgt cgtcgacagc tgcgtcgccg tcgacacccc gaccaccgcc cccgcaccgt
     3901 cgacaccatg agcgatccga cggaggccga cggcgacccc gtggtcgcgc tgagcccgct
     3961 ggagctgctc ctcgccgtcc aggaccagga caccgcctcg gaccagttgg tgcaccgccg
     4021 ctcccacctc cccgagcgcc aggccgtcga cgaccacgcg gcggtcacgg ccgcggtcga
     4081 cgccgagcgg gcgacacggg agacccggcg ggaggagctg gcccgcaaca tcaagcgcct
     4141 tcaggacgag gtggcttc
//
LOCUS       DASTFV010000298         3500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1123494, whole genome shotgun sequence.
ACCESSION   DASTFV010000298 DASTFV010000000
VERSION     DASTFV010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123494"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VFP02_05670"
     CDS             <1..945
                     /locus_tag="VFP02_05670"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HET9542545.1"
                     /translation="DIGWLADGLYGIAAAAYGVLVVLTLLRLVGFGRLLVADLTSHAK
                     GFAFLTTVAATNVLGSASAIVHGWWDLAWVLWWFSLGLWAVLAYSTLIAVVLRSPKPG
                     LGAGINGTWFLLTVSTESIAVLGALLLVRHPSDGLAFACIGAFLLGIVLYLIVMTMVF
                     QRWTFQDLEPTEADPPAWIAAGAVAITVLAGSNLLGAAPVSVRIDRLGPALEALVMLA
                     WATATFWFPLMIAIGVWRHVVRRVPLRYHPSYWSMVFPLGMYGAATFRMRAALELEVL
                     AWLPKVTLAVALLAWTATFIGLVHAGLNATERALRAGS"
     gene            1035..2696
                     /locus_tag="VFP02_05675"
     CDS             1035..2696
                     /locus_tag="VFP02_05675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9542546.1"
                     /translation="MRGERLGASLGAARAVLARPQLRRAELAFAAALTGEWAVTVALA
                     VVAFDDGGAAGVGLVALIRMVPSAVGTPVITAYSDRTRRERALATVSILRAVTIGGAA
                     VVLAVDAPHVFMYALVVLATAAFTVFRPTHSSLLPLLCTTTAELTGANVVRGVLESLA
                     TLVGPVVAGALLAASGPASAFGAAAVFSLMAAGLLLRIDYDAPARTEPTERSRLVEGT
                     ADGLKAVAGHRDLRLLFGLGFAQTYVRGSLNVFTVVMAFELLDLGDSGVAALTAAVGV
                     GGLIGALGVSLLVGSRHLGVWLMIALVLWGAPLAVIGAVPVTAVAFALIAVIGVANAI
                     IDVPLFTLPVRLVRDDVLTRVFGVFESIITIGVALGSALTPVVIALLGLEGAMIATGA
                     LLPVIALLAWRPLVALDERLGVRDDEIDALRRTPMLQLLPVPSIEYLASRVDTHPVAA
                     GSDLCRQGGPGDSYFVIVAGEADVIGDGETVASIGPGAGVGEIALLRDVPRTATVRAR
                     ENLVVMEIERQVFLEVVTGHSTTHEAANEVVAQHLADYHPARVGI"
     gene            2698..>3500
                     /locus_tag="VFP02_05680"
     CDS             2698..>3500
                     /locus_tag="VFP02_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET9542547.1"
                     /translation="MAPRRILGVFAHPDDEVFCVGGTMATYVDAGAEAMIVSATRGEA
                     GQIRDAASATRATLGEARERELRAACRCLGVQDVRLLDHLDGTLAGLDQEAFIAEVGA
                     VIDEFGPDVVVTFGDDGFYGHPDHVTIGVVVTAACARRPDTRLFHSHFPRTRLLLLER
                     LAEWLVELQGRFEGPADFARVFSLFAMETSAVGYADDHIEVAWFPPGMAIIEQGEQAD
                     TLYLILAGEVEVAQDQPDGSRTIIRRQGPGEFFGELGVAHQEVRTAHVIS"
BASE COUNT          391 a         1324 c         1200 g          585 t
ORIGIN      
        1 gacatcgggt ggctggccga cggcctctac gggatcgccg cggcggcgta cggggtcctg
       61 gtcgtgctca cccttctccg gctggtcggg ttcggtcgcc tcctcgtggc cgacctcacc
      121 agccacgcca agggcttcgc gttcctcacc accgtcgccg ccaccaacgt gctgggcagc
      181 gcctctgcca tcgtccacgg ctggtgggac ctggcctggg tcctctggtg gttcagcctc
      241 gggctgtggg cggtgctggc ctacagcacg ctgatcgccg tcgtcctccg gagcccgaag
      301 cccggcctcg gggccggcat caacggcacg tggttcctgc tgacggtctc gacggagtcc
      361 atcgccgtgc tcggcgccct cctgctcgtc cgccacccca gcgatggcct ggccttcgcc
      421 tgcatcggcg ccttcctgct cgggatcgtg ctctacctga tcgtgatgac catggtgttc
      481 cagcgctgga ccttccagga cctggagccg accgaggccg acccaccggc ctggatcgcg
      541 gccggtgccg tcgccatcac cgtcctggcc ggctccaacc tcctcggggc tgcacccgtc
      601 tcggtccgga tcgaccgcct tggccctgcc ctcgaggcac tggtcatgct ggcttgggcc
      661 acggccacgt tctggttccc cctgatgatc gccatcggcg tgtggcgcca cgtcgtacgc
      721 cgcgtaccgc tgcgctacca cccctcctac tggtcgatgg tgttccccct cggcatgtat
      781 ggcgccgcca ccttccgcat gcgagccgcg ctcgagctcg aggtcctggc ctggctgccg
      841 aaggtcacgc tcgccgtggc gctcctcgcc tggaccgcga ccttcatcgg gctcgtccac
      901 gccggcctca acgcgacgga gagggctttg cgggccgggt cgtgaggacc agccggtcga
      961 tggggtggtg gccgcccctc ctccctcgtg ctgaccccct acaggcggtg ccggacccgt
     1021 cgtaggttga cctcatgagg ggggagcgcc tgggcgcgtc gttgggtgcg gcccgggcgg
     1081 tgctcgcccg cccccagctg cgccgagcgg agctggcgtt cgccgccgca ctcacgggcg
     1141 agtgggccgt caccgtggcc ctcgccgtgg tggccttcga cgacggcggg gcagccggcg
     1201 tcggcctggt cgccctcatc cgcatggtcc cctcggccgt cggcacgccc gtcatcaccg
     1261 cctactccga tcggacccga cgggagcggg ccctggcgac cgtgtcgatc ctccgggcgg
     1321 tcaccatcgg aggcgccgcg gtcgtactcg ccgtcgacgc gccccacgtg ttcatgtatg
     1381 ccctcgtcgt cctggccacc gcggcgttca cggtgttccg ccccacccac tcatcgctgc
     1441 tgcccctgct ctgcacgacg acggccgaac tgaccggcgc caacgtcgtg cggggcgtgc
     1501 tggagtcgct ggccacactg gtcgggccgg tcgtagccgg cgccctcctc gccgccagcg
     1561 ggccggcgtc ggccttcggc gcggcggcgg tgttctcgct gatggcggcc ggtctcctgc
     1621 tgcggatcga ctacgacgcc ccggcccgca ccgaaccgac ggagcggtcc cgcctcgtcg
     1681 aggggacggc cgacgggctg aaggcggtgg cggggcaccg cgatctccga ctgctcttcg
     1741 ggctcggctt cgcccagacc tacgtccggg gctcgctcaa cgtgttcacg gtggtcatgg
     1801 ccttcgagct cctggacctc ggtgactcgg gcgtcgccgc cctgaccgcc gccgtcggcg
     1861 tcggtggcct catcggggcc ctcggcgtgt ccctgctggt gggcagtcgg cacctcgggg
     1921 tctggctgat gatcgccctc gtgctgtggg gcgccccgct cgccgtcatc ggcgccgtgc
     1981 ccgtcactgc agtggcgttc gccctcatcg ccgtcatcgg cgtggccaac gccatcatcg
     2041 acgtgcccct cttcacgctg ccggttcggc tggtgcgcga cgacgtgctg acccgggtgt
     2101 tcggggtgtt cgagagcatc atcaccatcg gggtcgccct cggctcggcc ctgacccctg
     2161 tggtcatcgc cctcctcgga ctggaggggg cgatgatcgc caccggcgcc ctgctccctg
     2221 tcatcgctct cctggcctgg cgccccctcg tggcgctcga cgaacgactc ggggtccgcg
     2281 acgacgagat cgacgccctg cgccggaccc cgatgctcca gctcctcccg gtgccgagca
     2341 tcgagtacct cgcctcgcgg gtggacaccc acccggtcgc ggccgggtcc gatctgtgcc
     2401 ggcagggcgg tcccggagac agctacttcg tgatcgtggc cggcgaggcc gacgtcatcg
     2461 gcgacggcga gaccgtggcg tcgatcgggc cgggcgccgg ggtgggcgag atcgccctcc
     2521 tccgggacgt tccccggacc gcgacggtcc gagcccgcga gaatctcgtc gtcatggaga
     2581 tcgagcgaca ggtgttcctc gaggtggtga ccggtcacag cacgacccac gaagccgcca
     2641 acgaggtcgt ggcccagcac ctggccgact accacccggc ccgggtcggg atctgacgtg
     2701 gccccccgtc gcatcctcgg cgtcttcgcc catcccgacg acgaggtctt ctgcgtcggc
     2761 gggacgatgg cgacctacgt ggatgccgga gccgaggcca tgatcgtgtc ggccacccgg
     2821 ggcgaggccg gccagatccg ggacgccgcc tccgccaccc gggccaccct cggcgaggcc
     2881 cgggagcggg agctgcgggc ggcctgccgg tgcctcggcg tgcaggacgt ccgtctgctc
     2941 gaccacctgg acggcaccct ggccggcctc gaccaggagg ccttcatcgc ggaggtcggc
     3001 gccgtcatcg acgagttcgg ccccgacgtc gtggtcacct tcggtgacga cgggttctac
     3061 ggccatcccg accacgtgac catcggcgtc gtcgtcaccg cggcctgcgc ccggcgaccg
     3121 gacacccggc tgttccactc gcacttcccg cggacccgcc tcctgctgtt ggagcgcctg
     3181 gcggagtggc tggtcgagct gcagggacgt ttcgagggcc ccgccgactt cgcccgggtg
     3241 ttctccctgt tcgcgatgga gacgagcgcc gtcggctacg ccgacgacca catcgaggtc
     3301 gcctggttcc cgccgggcat ggccatcatc gagcagggtg agcaagcgga caccctgtac
     3361 ctcatcctcg ccggcgaggt ggaggtggcc caggaccagc ccgacgggtc gcggacgatc
     3421 atccgccggc aggggccggg cgagttcttc ggcgagctcg gcgtggccca ccaggaggtc
     3481 cgcaccgccc acgtgatctc
//
LOCUS       DASTFV010000299         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1127334, whole genome shotgun sequence.
ACCESSION   DASTFV010000299 DASTFV010000000
VERSION     DASTFV010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127334"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2229
                     /gene="rpoB"
                     /locus_tag="VFP02_05685"
     CDS             <1..>2229
                     /gene="rpoB"
                     /locus_tag="VFP02_05685"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET9542548.1"
                     /translation="FRLRNLAKHQLVTGTIHPYRGEWIEFDVEQKPGKDVTCGTRVAR
                     KRRLSIFVLLRALGYDEENEPGFIQRFVDHFDFLEGQWEKEREIAQTQDEALVEIYKR
                     ARPGEPPTTESARAYFRNAFFEPRRYDLSRVGRYKLNRKLGPELQNLQDTFGLKDLDL
                     PAADQPVLSRCEVLAACTYLLHLAKGDPGYRLDDQDHFANRRIRSVGELIQNQVRIGL
                     SRMERVVRERMTTQDVEAITPQTLINIRPVVAAIKEFFGTSQLSQFMDQVNPLSGLTH
                     RRRLSALGPGGLSRERAGFEVRDVHFSHYGRMCPIETPEGPNIGLIGALATYARVNEF
                     GFLETPYRKVIDGRVTDEIVFLTADEEEEYVCAQANAPIDAKGNFRNDRVLVRRSPQA
                     ASLADLRLMLERDVYFAATTEISSVPPKEVQMMDISPKQIVSVATSLIPFLEHDDANR
                     ALMGANMQKQAVPLVRAEAPFIGTGVEARAARDAAEMILAEDAGTILEVDGKFIEVEY
                     AKLGRKVYKLLKFERSNQDTAINQKPRVVEGQKVKKGDLLADGPSTDQGELALGKNLL
                     VAFMPWEGYNFEDAIILSERLVRDDVLTSIHIHEYEIDARDTKLGPEEITRDIPNLSD
                     DILADLDERGIVRVGAEVGPGDVLVGKVTPKGETELTPEERLLRAIFGEKAREVRDTS
                     LKVPHGEQGKVIDVKLFSRDEQHELPPGVNQLVRVYVGQKRKISVGDKLAGRHGNKGV
                     IS"
BASE COUNT          400 a          815 c          713 g          301 t
ORIGIN      
        1 cttccgcctg cggaacctgg ccaagcacca gctcgtcacc ggcaccatcc acccctaccg
       61 gggtgagtgg atcgagttcg acgtcgagca gaagccgggc aaggacgtca cctgcggcac
      121 ccgcgtggcc cgcaagcgtc gcctcagcat cttcgtgctc ctccgcgccc tcggctacga
      181 cgaggagaac gagcccggct tcatccagcg cttcgtcgac cacttcgact tcctcgaggg
      241 gcagtgggag aaggagcggg agatcgccca gacccaggac gaggccctgg tcgagatcta
      301 caagcgggcc cgccccggcg agccgcccac caccgagtcg gcgcgcgcct acttccgcaa
      361 cgccttcttc gagccgcggc gctacgacct cagccgggtc ggccgctaca agctgaaccg
      421 caagctcggc cccgagctgc agaacctgca ggacaccttc ggcctgaagg acctggacct
      481 gcccgccgcc gaccagcccg tgctgagccg ctgcgaggtc ctcgccgcct gcacgtacct
      541 cctgcacctg gccaagggcg atccgggcta ccgcctggac gaccaggacc acttcgccaa
      601 ccggcgcatc cgctcggtcg gcgagctcat ccagaaccag gtccgcatcg gcctctcccg
      661 catggagcgg gtggtgcgcg agcgcatgac cacccaggac gtcgaggcca tcacgcccca
      721 gacgctgatc aatatccggc cggtggtcgc cgccatcaag gagttcttcg gcaccagcca
      781 gctgagccag ttcatggacc aggtcaaccc cctgtcgggc ctgacccacc gtcggcgcct
      841 cagcgccctc ggcccgggtg gcctgtcccg ggagcgggcc ggcttcgagg tccgagacgt
      901 ccacttcagc cactacggcc gcatgtgccc gatcgagacg ccggagggcc cgaacatcgg
      961 cctcatcggc gccctggcca cctacgcccg ggtcaacgag ttcggcttcc tcgagacgcc
     1021 gtaccggaag gtcatcgacg gtcgcgtcac cgacgagatc gtgttcctga ccgcggacga
     1081 ggaagaggag tacgtctgcg cccaggccaa cgcgcccatc gacgccaagg gcaacttccg
     1141 caacgaccgg gtcctggtgc ggcgctcacc gcaggccgcc tcgctggccg acctgcgcct
     1201 catgctcgag cgcgacgtct acttcgcggc gaccaccgag atcagctcgg tgccgcccaa
     1261 ggaagtccag atgatggaca tctcgccgaa gcagatcgtc tcggtggcca cgtcgctcat
     1321 ccccttcctc gagcacgacg acgccaaccg ggccctgatg ggcgccaaca tgcagaagca
     1381 ggcggtcccg ctggtgcggg ccgaggcgcc gttcatcggc accggggtcg aggcccgggc
     1441 cgcccgcgac gcggccgaga tgatcctggc cgaggacgcc gggaccatcc tcgaggtcga
     1501 cggcaagttc atcgaggtcg agtacgccaa gctgggtcgc aaggtctaca agctgctcaa
     1561 gttcgagcgc tcgaaccagg acaccgccat caaccagaag ccccgcgtcg tcgagggcca
     1621 gaaggtgaag aagggtgacc tgctcgccga cggcccctcc accgaccagg gcgagctggc
     1681 cctcggcaag aacctcctcg tggccttcat gccctgggag ggctacaact tcgaggacgc
     1741 catcatcctg tccgagcggc tggtccgtga cgacgtcctc acgtcgatcc acatccacga
     1801 gtacgagatc gacgcccgcg acaccaagct cggcccggag gagatcaccc gggacatccc
     1861 gaacctctcc gacgacatcc tggccgacct cgacgagcgc ggcatcgtgc gggtcggggc
     1921 cgaggtgggc cccggcgacg tcctggtcgg caaggtcacg cccaagggcg agaccgagct
     1981 gacccccgag gagcggctcc tgcgggccat cttcggggag aaggcccgtg aggtgcggga
     2041 cacttccctg aaggtgcccc acggcgagca gggcaaggtc atcgacgtca agctcttcag
     2101 ccgggacgag cagcacgagt tgccgcccgg cgtgaaccag ctggtgcggg tctacgtcgg
     2161 ccagaagcgc aagatcagcg tgggcgacaa gctggccggc cgccacggca acaagggcgt
     2221 gatctccaa
//
LOCUS       DASTFV010000300         4203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1136104, whole genome shotgun sequence.
ACCESSION   DASTFV010000300 DASTFV010000000
VERSION     DASTFV010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136104"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2945
                     /gene="nifJ"
                     /locus_tag="VFP02_05690"
     CDS             <1..2945
                     /gene="nifJ"
                     /locus_tag="VFP02_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HET9542549.1"
                     /translation="PDRPVLRGSAQNPDVFFQAREAANPFHAAVPGVVEEIFAELADR
                     TGRRYGLVEYQGHPEAERALVIMGSGAGAAGETVDAMVADDQKVGLLTVRLFRPFPTD
                     ALTAALPASVSRLAVLDRTKEPGSVGEPLYLDVLTALVEAQAAGARETVPTVVGARYG
                     LGSKEFTPADVKGLFDELGRDTRRPHVTVGIVDDVTGLSVEPDAGFSVPTGTVQAVFH
                     GLGSDGTVGATKATVKILGEHTNLHAQGYFVYDSRKAGAETESHLRFGPDPIASTYLI
                     DGADFVACHQFGFLGRRDVLSRARPGAIFLLNSPYPPDEVWDHLPGDVQDEIVDKGLT
                     LHVIDAERVAGEVGMGRRINTVMQPCFFALSGVLPTDQALAAIRARIEVTYAKAGPAV
                     VERNLAAVDAALAALHEVPVPITASAGDHRPTRVLVTAPDFVTRVTARLLAGEGDLLP
                     VSALPVDGTFPTGTARWEKRALATELPVWDEAICIDCGKCALVCPHAAIRMKVYDPAA
                     MTGAPDGFKAKDFRSRDLPGQRLTIQVAPDDCTGCGVCVQVCPAKAKDEVRHKALAMV
                     PVGDRRERERLAFEFFLDLPELDPVLLAPDSVKGSQVRQPLFEFSGACAGCGETPYLK
                     LLTQLFGDRMLVANATGCSSIYGGNLPTTPWSQDATGRGPAWANSLFEDNAEFGLGLR
                     LGLDQQVAEARRLVDALAPDLGEDLARELLAAEGRDAVAIAAQRDRVATLQERLAVRE
                     DPESVRLRGLADDLVARSVWIVGGDGWAYDIGFGGVDHVLASGRNVNLLVLDTEVYSN
                     TGGQASKATPRGAVAKFASGGRRTGKKDLGDDGRAYGDVYVAQVAMGASDVQTVKAFL
                     EAEAWPGPALVIAYSPCVAHGIDMSQTMVHMRDAVHSGHWPLYRYRPGAEEHEQPFQL
                     DSHTPDRPFREFAMTEGRFAMLARSDPERADQLFSLAQADVDERWRYYEQLAGIERTM
                     PPSEGDEP"
     gene            2942..3949
                     /locus_tag="VFP02_05695"
     CDS             2942..3949
                     /locus_tag="VFP02_05695"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HET9542550.1"
                     /translation="MTDLTTTYLGLPLRCPLVASSSPATGDLEGLRRLDEAGIGAVVL
                     PSLFEEQIVQEALEVDRMLATGAEAFAEATDYFPELDDYNTGPDHYLARVVEAKAALS
                     VPVIASLNAVSSGGWVRYGRLLADVGADALELNVNIVATDPVLSAAEVEAALLTTVGD
                     VLDAVDIPVALKLGPFWSSLPHLARRLAETGVRGLVLFNRPFAPDLDLEELVVVPHLT
                     LSEPDDILLPLRWIGLLEGQVDTDLAATSGVHDASSVAKVLLAGAQVAMMATALLRNG
                     PGHAAVVLDELRAWMAENDYASVDQLRGSASRRGVADPAAYERSNYLQTLTSYSSTFP
                     T"
     gene            complement(3979..>4203)
                     /locus_tag="VFP02_05700"
     CDS             complement(3979..>4203)
                     /locus_tag="VFP02_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542551.1"
                     /translation="FALDGILIGAGDLAFLAKAMIVAFLVFAPAAVAVLVLDLGIGWV
                     WAALALLFLARALVLGARFRGEAWIRLGAD"
BASE COUNT          607 a         1528 c         1460 g          608 t
ORIGIN      
        1 acccggaccg gccggtgctg cggggcagtg ctcagaaccc ggacgtcttc ttccaggccc
       61 gggaggccgc caaccccttc cacgccgctg tacccggcgt cgtcgaggag atcttcgccg
      121 aactggctga ccgcaccggt cgtcgctacg gcctggtcga gtaccagggt caccccgaag
      181 ccgaacgtgc gctcgtgatc atgggctcgg gggccggtgc cgccggtgag accgtcgacg
      241 ccatggtcgc ggacgaccag aaggtcggcc tgctgaccgt tcgcctgttc cgtcccttcc
      301 ccacggatgc cctgaccgcg gcgctgccgg cgtcggtgtc tcgcctggcc gtcctggacc
      361 ggaccaagga gccgggctcg gtcggggagc cgctctacct cgacgtcctg accgccctgg
      421 tcgaggccca ggctgctggt gcccgggaga cggtgcccac cgtggtcggt gcccgttacg
      481 gcctcggctc caaggagttc accccggccg acgtcaaggg cctcttcgac gaactgggcc
      541 gtgacacccg ccgcccccac gtgaccgtcg ggatcgtcga cgacgtgacc gggctgagcg
      601 tcgagccgga cgccgggttc tcggtgccca cgggcacggt ccaggcggtg ttccacggcc
      661 tcgggagcga cggcaccgtc ggtgccacca aagccaccgt gaagatcctg ggtgagcaca
      721 ccaacctgca cgctcagggc tacttcgtct atgactcccg caaggcgggg gccgagaccg
      781 agtcgcacct ccgcttcggg ccggacccga tcgcctcgac ctacctgata gacggggccg
      841 acttcgtggc ttgtcaccag ttcgggttcc tgggccgacg cgacgtgctg agccgggccc
      901 ggcccggggc catcttcctg ctcaacagcc cgtacccgcc cgacgaggtc tgggaccacc
      961 tgcccggtga cgtgcaagac gagatcgtcg acaagggcct gaccctccac gtcatcgacg
     1021 ccgagcgggt ggcgggggag gtgggcatgg gccggcgcat caacacggtc atgcagccct
     1081 gcttcttcgc cctctccggt gtcctgccca cggaccaggc gctggccgcc atccgggctc
     1141 gtatcgaggt gacctatgcc aaggcaggcc cggccgtcgt cgagcgcaac ctggccgcgg
     1201 ttgacgccgc actggccgcc ctgcacgagg tgcccgttcc gatcacggcg tcggccgggg
     1261 accatcgacc gacccgggtg ctggtcactg cgcccgattt cgtcacccgc gtcaccgctc
     1321 ggctcctggc cggcgagggc gacctcctgc cggtcagcgc cctccccgtc gacgggacct
     1381 tcccgacggg cacggcacgc tgggagaagc gggcgctggc caccgagctc ccggtgtggg
     1441 acgaggcgat ctgcatcgac tgcggcaagt gcgcgctggt ctgcccgcac gccgcgatcc
     1501 gcatgaaggt ctacgacccc gcggcgatga cgggcgcgcc ggacggcttc aaggccaagg
     1561 acttccgctc ccgcgacctg cccgggcaac ggctgaccat ccaggtggcg cccgacgact
     1621 gcaccggatg cggcgtctgt gtccaggtgt gcccggccaa ggcgaaagac gaggtacggc
     1681 acaaggcgtt ggccatggtt cccgtcggtg accggcggga gcgggaacgg ctggccttcg
     1741 agttcttcct cgacttgccg gagttggatc ctgtcctcct cgccccggac tcggtgaagg
     1801 gctcacaggt tcgccagccg ctcttcgagt tctccggggc ctgtgcgggt tgcggcgaga
     1861 ccccgtacct caagctgctg acccagctct tcggcgaccg gatgctggtg gccaacgcca
     1921 ccggctgctc gtcgatctac ggcggcaacc tgccgaccac gccgtggtcc caggatgcca
     1981 ccggtcgggg tccggcctgg gccaactccc tcttcgagga caacgccgag ttcggcctcg
     2041 ggctgcgcct gggcctggac cagcaggtgg ccgaggcccg ccgcctggtc gacgccctcg
     2101 cccccgacct cggtgaggac ctggcccgtg agctgctggc ggccgagggc cgtgacgccg
     2161 tggcgatcgc ggcccagcgg gaccgggtgg caacactgca ggagcgccta gctgtacgtg
     2221 aggatcccga gagcgtccgc cttcggggcc tcgccgacga cctggtggcc cgcagcgtgt
     2281 ggatcgtggg cggcgacgga tgggcctacg acatcggctt cggtggcgtc gatcacgtcc
     2341 tggccagtgg ccgcaacgtc aacctcctcg tcctcgacac cgaggtgtac tccaacactg
     2401 gcggccaggc ctccaaggcg acgccacggg gcgcggtggc caagttcgcc tccggcgggc
     2461 ggcggacggg caagaaggac ctgggcgacg acgggcgggc ctacggcgac gtctacgtgg
     2521 cccaggtggc catgggggcc agtgacgtgc agacggtgaa ggccttcctg gaggccgagg
     2581 cttggccggg accggccctg gtgatcgcct acagcccctg tgtggcccac ggcatcgaca
     2641 tgagccagac gatggtgcac atgcgcgacg ccgtgcactc cggtcactgg ccgctctacc
     2701 gctaccgccc cggggccgag gagcacgagc agccgttcca gctcgacagc cacaccccgg
     2761 accggccctt ccgggagttc gccatgaccg aggggcgatt cgccatgctg gcccgcagcg
     2821 acccggagcg ggccgaccaa ctgttctccc tggcccaagc cgacgtcgac gagcggtggc
     2881 gctactacga gcagctggcc gggatcgagc ggacgatgcc accgtcggag ggggacgagc
     2941 catgaccgat ctgaccacca cctacctcgg gctgcccctc cggtgcccac tggtcgcctc
     3001 ctccagcccg gccacgggcg atctcgaggg actgcgtcgc ctcgacgagg ccggcatcgg
     3061 cgcggtcgtg ctgccgtccc tgttcgagga gcagatcgtc caggaggccc tcgaggtcga
     3121 ccgcatgctg gccacaggcg cggaggcttt cgcagaggcg accgactact tccccgagct
     3181 cgacgactac aacaccgggc ctgaccacta tctggcccgg gtggtcgagg cgaaggccgc
     3241 gctgtcggtc cctgtcatcg ccagcctcaa cgccgtcagc agcgggggct gggtgcgcta
     3301 cgggcgcctg ctggccgacg tcggcgccga cgcgctcgag ctgaacgtga acatcgtggc
     3361 caccgacccc gtcctgtccg cggccgaggt tgaggccgcc ctgctgacga cggtcggtga
     3421 tgtgctcgac gccgtcgaca tcccggtggc cctgaagctg gggccgttct ggtcctcgct
     3481 cccgcacctc gcccggcggc tggccgagac cggtgtgcgg ggcctcgtcc tgttcaaccg
     3541 tcccttcgcc cccgacctgg acctggagga gctggtcgtc gttccccacc tcacgctgag
     3601 tgagccagac gacatcctgc tgccgctgcg ctggatcggc ctgctcgagg gccaggtcga
     3661 caccgacctg gccgccacca gcggggtgca cgacgcctct tcggtggcga aggtcctcct
     3721 ggcgggagcc caggtggcca tgatggccac cgccctcctc cgcaacggcc ccggtcacgc
     3781 ggccgtggtc ctcgacgagc tgcgggcgtg gatggccgag aacgactacg cgtcggtgga
     3841 ccagctccgg ggctcggcct ctcggcgagg ggtcgccgac cccgccgcgt acgagcggtc
     3901 caactacctc cagacgctca ccagctactc ctcgaccttc cccacctgag gcgcagagca
     3961 ccggtccggg cccgaaggtc agtcggcgcc caggcggatc caggcctcgc cccggaaccg
     4021 ggcacctagg acgagggccc gagcgaggaa gagcagggcc aaggcggccc acacccagcc
     4081 gatgcccagg tcgaggacca ggacggcgac agcggcgggg gcgaacacga ggaaggcgac
     4141 gatcatggcc ttcgcgagga acgccaggtc gccggcgccg atgaggatgc cgtcgagggc
     4201 gaa
//
LOCUS       DASTFV010000301         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1137308, whole genome shotgun sequence.
ACCESSION   DASTFV010000301 DASTFV010000000
VERSION     DASTFV010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137308"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VFP02_05705"
     CDS             <1..567
                     /locus_tag="VFP02_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HET9542552.1"
                     /translation="DPDPVKAETEGKPMPGVELKLVKLDGTVAGPDEEGEIRAKAPQM
                     MRGYLDSSLDADAFDEDGWFRTGDLGQQDAEGNLIITGRLKDIIIRNMENISAKEVED
                     LLFEHPQVGDVAVIGLPDERTGERVVAIVVTAEGQDPISFETVKEHLLAKGLRKQALP
                     EQLEFMDVLPRNPTGKVVKYELRDQFAD"
     gene            644..1933
                     /locus_tag="VFP02_05710"
     CDS             644..1933
                     /locus_tag="VFP02_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542553.1"
                     /translation="MTERTASDLLAAGLEALGVTRVFGEPTAVPWPGLTTVPVADGVI
                     ADLLADVDGRLDRVGASWHDRVLRVSSRPGALVESVRIDSAESLANALATTLGQEVPD
                     AFALDLDLDLAGPVPDGIEPKAALTRIEGIRLSPGLAPDLGVVVGPAVLRVLDGEGVV
                     AHLQRFAAQTGLPVVNTWGAKGVFPWDSPFHAGTAGLQARDFELAGLTDKLTVVCIGV
                     DPDELPPGGFGDAQRLDLHPVHLAFAAEGWPEPEREPPRPPLYTDLAAVVGPAYESEA
                     VPLHPARASRDLGALRPFGGVVVADPGPAGLWVARTFPTTEPGSVLVPGTNAPGTAAA
                     AALLGGLDASRPALGVTTWPLDPMTEALLDLARSLEVDLTLEAWGGEADLSSPEDRQE
                     ATAAAIARSGVDVVPVPVDFRATADLVAVAGEISAWS"
     gene            1965..>2151
                     /locus_tag="VFP02_05715"
     CDS             1965..>2151
                     /locus_tag="VFP02_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9542554.1"
                     /translation="MTRSFAFADLWELVAEQVPDREAVVCRDRRLTYGDLDERANRLA
                     AHLRANGTRPGDHVGVYL"
BASE COUNT          282 a          824 c          755 g          290 t
ORIGIN      
        1 gaccccgatc ccgtgaaggc cgagaccgag ggcaagccca tgcccggcgt cgagctgaag
       61 ctggtgaagc tggacggcac ggtcgccggt cccgacgagg agggcgagat ccgggccaag
      121 gccccgcaga tgatgcgggg ctacctggac tcgtccctgg acgccgacgc cttcgacgag
      181 gacggctggt tccgcaccgg tgacctcggc cagcaggacg ccgagggcaa cctgatcatc
      241 accgggcgcc tgaaggacat catcatccgg aacatggaga acatctcggc caaggaggtc
      301 gaggacctcc tcttcgagca cccccaggtc ggcgacgtcg ccgtcatcgg cctccccgac
      361 gagcgcaccg gcgagcgggt cgtcgccatc gtggtcaccg ccgagggcca ggacccgatc
      421 agcttcgaga ccgtgaagga gcacctgctg gccaagggcc tccgcaagca ggccctgccg
      481 gagcagctcg agttcatgga cgtcctcccg aggaacccca ccggcaaggt cgtcaagtac
      541 gagctccggg accagttcgc cgactgaccc cccgttctgg cctccgattt accggccctg
      601 accggcagat cagaggccag aagggggctt accctcggcg gcgatgactg agcgcaccgc
      661 ttcggatctg ctggccgccg ggctcgaggc cctcggggtg actcgggtgt tcggggagcc
      721 gaccgcggtg ccgtggccgg ggctgacgac cgtgccggtc gccgacgggg tcatcgccga
      781 cctgttggcc gacgtcgacg ggcggctgga ccgggtgggg gcgtcgtggc acgaccgagt
      841 cctgcgggtc agctcccggc cgggcgccct cgtcgagtcg gtccgcatcg acagcgccga
      901 gagcctggcc aacgccctgg ccaccaccct gggccaggag gtcccggacg ccttcgccct
      961 ggacctggac ctggacctgg ccggtccggt gcccgacggg atcgagccga aggcggccct
     1021 gacccggatc gagggcatcc gcctgtcgcc cgggctcgcc cccgacctcg gtgtcgtggt
     1081 cggaccggcc gtgctccggg tcctggacgg cgagggcgtg gtcgcccacc tccagcgctt
     1141 cgccgcccag accggcctcc cggtggtcaa cacctggggg gccaagggcg tgttcccctg
     1201 ggacagcccg ttccacgccg gcaccgcggg cctgcaggcc cgggacttcg agctggccgg
     1261 cttgaccgac aagctgaccg tggtctgcat cggcgtcgac cccgacgagc tgcccccggg
     1321 cggcttcggt gacgcccagc gcctggacct ccatccggtg cacctggcct tcgccgccga
     1381 gggctggccc gagccggaga gggagccgcc ccgcccgccg ctctacaccg acctggccgc
     1441 cgtggtcggc cccgcctacg agtccgaggc ggtgccgctg cacccggccc gagccagtag
     1501 ggatctcggc gccctccgtc ccttcggtgg cgtcgtcgtg gccgatcccg gccccgccgg
     1561 gctgtgggtg gcccgcacct tcccgacgac cgagcccggt tcggtcctcg tgccgggcac
     1621 gaacgccccc ggcaccgcgg ccgccgccgc cctcctcggg ggcctcgacg cgtcccggcc
     1681 cgccttgggc gtgacgacgt ggccactcga cccgatgacg gaggcgctgc tcgacctggc
     1741 ccgctcgctc gaggtcgacc tcaccctcga ggcctggggc ggcgaggccg acctgtcctc
     1801 gcccgaggac cggcaggaag ccaccgccgc cgccatcgcc cgctccggcg tggacgtcgt
     1861 ccccgtcccc gtcgacttcc gcgccaccgc cgacctggtc gccgtcgccg gcgagatcag
     1921 cgcctggtcc tgaccgccgc gcctcgcgtc tacggtcccc ggccatgacc cggtcgttcg
     1981 ccttcgccga tctgtgggag ttggtggccg agcaggtgcc ggaccgggaa gcggtggtct
     2041 gccgtgaccg gcggctgacc tacggggatc tcgacgagcg agccaaccgg ctggccgccc
     2101 acttgcgggc caacgggacc cgccccggtg accacgtcgg cgtctacctg a
//
LOCUS       DASTFV010000302         2877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1137696, whole genome shotgun sequence.
ACCESSION   DASTFV010000302 DASTFV010000000
VERSION     DASTFV010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137696"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VFP02_05720"
     CDS             <1..585
                     /locus_tag="VFP02_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9542555.1"
                     /translation="LVCANAGVFQGGISWERSVEDWQWVLGVNLWGVIHAVKAFVPRM
                     IEGGDEGHVVVTSSMAGLACAAFSGPYQTSKFGAYALAESLAHDLRTSGIPIGVSALC
                     PGAVNTRIAGSNRNRPGALVAKGGEDVDFVEGALADLTSQQGIDPSEVAARVVAGVQA
                     GDFLIPTNDAYADQLRVHTDAQIARQLPPMADFS"
     gene            complement(652..1431)
                     /locus_tag="VFP02_05725"
     CDS             complement(652..1431)
                     /locus_tag="VFP02_05725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9542556.1"
                     /translation="MGRLDGRVAIITGAGGGIGFEYAKRFLGEGARVAIAEVDEDTCR
                     SAAEELAALGDVEPVPTDIADEASVQAGVDAVRERFGSVDILVNNAAIYGNLDIGDQS
                     LDYLRTVFDVNLHGQWLMARAVAPFMVEQGRGSIVNIAAIAAFLHQINAFDPEFNGVG
                     NYAYAQSKWGVIGLTHHLAGQLGQFGVTVNCIAPGLTMTEATKTTVPDFAMPFFEQLS
                     PMKRNVQPDQLTGAALFFASDDAALVNGQIICVDGGTVMPV"
     gene            1454..2560
                     /locus_tag="VFP02_05730"
     CDS             1454..2560
                     /locus_tag="VFP02_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542557.1"
                     /translation="MLTPGDDFPIHQVAEPIRHVGTSDRNFYDRYYFNAHNCSGDLFL
                     VLGLGLYPNLGTQDAFAVVRRGEKHRVVRSSRVLDDRMDLTTGPLRVEVVEPLRKIRF
                     VLEPTEHPLALDMTFEGAHAAFEEPKQLIRKFGRLLFDTARFCQNGRWSGSLEVDGES
                     FTVEPDTWWGMRDRSWGVRPVGEAEPPGIRGQTGQMVGLWNYAPMQFEDHQIFYICQE
                     EPDGTRTLEEAIRVWNDPAREPDPLGRPEWEHTLVSGTRRIDTPSTLSFPEAPGGGFT
                     VTVNPLVNAYVAIGTGYGIEDDWRHGMYQGDLVTQYVEHDMAELDTWAWFSLNEQVAR
                     FERSDGPVGYGMHEYGVFGPFPKFGLSDGAAVAL"
     gene            complement(2602..>2877)
                     /locus_tag="VFP02_05735"
     CDS             complement(2602..>2877)
                     /locus_tag="VFP02_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9542558.1"
                     /translation="AARAKEIGLAWKVVPDGQLMDEARTLAVRLTRGAPLAQRAIKEM
                     ASRGRELPWIDAVRFGETMRKVAGATEDATEGLSAAAERRDPHWKGR"
BASE COUNT          418 a         1043 c          986 g          430 t
ORIGIN      
        1 ctggtgtgcg ccaacgccgg cgtgttccag ggcgggatca gctgggagcg cagcgtcgag
       61 gactggcagt gggtgctggg cgtgaacctc tggggcgtca tacacgcggt gaaggcattc
      121 gtcccccgca tgatcgaggg gggcgacgag ggccacgtcg tggtcacgtc ctcgatggcc
      181 ggcctggcct gtgccgcctt cagcgggccc taccagacgt ccaagttcgg ggcctacgcc
      241 ctggccgagt ccttggccca cgacctgcgg acgtccggca tcccgatcgg tgtgtccgcc
      301 ctctgccccg gcgccgtcaa cacccgcatc gccggctcca accgcaatcg ccccggcgcg
      361 ctggtggcca agggcggcga ggacgtcgac ttcgtcgagg gagccctggc cgacctgacc
      421 agccagcagg gcatcgaccc gtccgaagtc gccgcccggg tcgtcgccgg cgtgcaggcc
      481 ggcgacttcc tcatccccac caacgacgcc tacgccgacc agctccgggt ccacaccgac
      541 gcccagatcg cccgccagct ccccccgatg gccgacttca gctgaagccg actcgtacct
      601 tgcgcgccac ggcgccagac ggggcgggcg ggtgcacgag gtagggggcc actagacggg
      661 catgacggtg ccgccgtcga cgcagatgat ctggccgttg accagggcgg cgtcgtcgga
      721 ggcgaagaac agggcggccc cggtcagctg atcgggctgc acgttgcgct tcatggggct
      781 gagctgctcg aagaagggca tggcgaagtc gggcacggtc gtcttggtgg cctcggtcat
      841 cgtcaggccg ggtgcgatgc agttcacggt caccccgaac tggcccagct ggcccgccag
      901 gtggtgggtc aggccgatca cgccccactt gctctgggcg taggcgtagt tgcccacccc
      961 gttgaactcg gggtcgaagg cgttgatctg gtgcaggaag gcggcgatcg cggcgatgtt
     1021 gacgatgctc ccgcggccct gctccaccat gaacggcgcc acggcccggg ccatgagcca
     1081 ctggccgtgg aggttcacgt cgaacaccgt gcgcaggtag tcgaggctct gatcgccgat
     1141 gtccaggttc ccgtagatgg cggcgttgtt gaccaggatg tccacgctcc cgaaccgctc
     1201 ccggaccgca tccacgccgg cctggaccga agcctcgtcg gcgatgtcgg tgggcacggg
     1261 ctcgacatcg cccagcgcgg ccagctcctc ggcggccgag cgacacgtgt cctcgtccac
     1321 ttcggcgatg gcgacgcgag cgccctcgcc caggaaacgc ttggcgtact cgaagccgat
     1381 gccccctccg gcgcccgtga tgatggccac tcgcccgtcc agtcgtccca tgggggcaac
     1441 gtagtctcga cggatgctca ctccgggaga cgacttcccc atccaccagg tggccgagcc
     1501 catccgccac gtcgggacca gcgaccgcaa cttctacgac cgctactact tcaacgccca
     1561 caactgctcg ggcgacctct tcctggtgct gggcctgggc ctctacccga acctcggcac
     1621 ccaggacgcc ttcgccgtgg tccgccgcgg cgagaagcac cgggtcgtgc gctccagccg
     1681 agtgttggac gaccgcatgg acctgaccac gggcccgctg cgggtcgagg tggtggagcc
     1741 gctgcgcaag atccgcttcg tgctggagcc gacggagcat cccctggccc tggacatgac
     1801 cttcgagggg gcccacgccg ccttcgagga gcccaagcaa ctcatccgca agttcggccg
     1861 gctcctcttc gacaccgccc gcttctgcca gaacgggcgc tggtcgggat ccctggaggt
     1921 ggacggcgag tccttcacgg tcgagcccga cacgtggtgg ggcatgcggg accggtcgtg
     1981 gggcgtccgc ccggtcggcg aggccgagcc gcccgggatc cggggccaga cgggccagat
     2041 ggtcgggctc tggaactacg cccccatgca gttcgaggac caccagatct tctacatctg
     2101 ccaggaggag cccgacggca cccgcacgct cgaggaggcg atccgggtgt ggaacgaccc
     2161 ggcccgggag cccgacccgc tgggccggcc cgagtgggag cacaccctgg tcagcgggac
     2221 ccgccggatc gacaccccgt cgacgctgtc cttccccgag gcgcccggcg gcggcttcac
     2281 ggtgacggtg aacccgctgg tcaacgccta cgtggccatc gggaccggct acggcatcga
     2341 ggacgactgg cgccacggca tgtaccaggg cgacctggtc acccagtacg tcgagcacga
     2401 catggccgag ctcgacacct gggcctggtt ctccctcaac gagcaggtgg cccgcttcga
     2461 gcgctccgac ggccccgtgg gctacggcat gcacgagtac ggcgtcttcg gccccttccc
     2521 caagttcggc ctcagcgacg gagcggccgt cgccctctga ccggtttcgc tccacccgcc
     2581 tgagcacccg acacctccgg gtcagcggcc tttccagtgg gggtcgcggc gttcggcggc
     2641 ggcgctgagg ccctcggtgg cgtcctcggt ggccccggcg accttgcgca tggtctcgcc
     2701 gaagcgcacc gcgtcgatcc aggggagctc ccgaccgcga ctggccatct ccttgatcgc
     2761 ccgctgcgcc aggggcgcgc cccgcgtgag gcggacggcc agggtgcggg cctcgtccat
     2821 cagctggccg tcggggacga ccttccaggc caggccgatc tccttggccc gagccgc
//
LOCUS       DASTFV010000303         3584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1142171, whole genome shotgun sequence.
ACCESSION   DASTFV010000303 DASTFV010000000
VERSION     DASTFV010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142171"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VFP02_05740"
     CDS             complement(<1..338)
                     /locus_tag="VFP02_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET9542559.1"
                     /translation="MATTAEAIRSGDRRALAQGITLVESVRPDHRAEAVALVEELLPA
                     SGGAVRVGVTGQPGAGKSTFIEALGLHLVDAGHRVAVLAVDPSSTRSGGSILGDKTRM
                     GELGRRDEAF"
     gene            complement(346..2469)
                     /gene="scpA"
                     /locus_tag="VFP02_05745"
     CDS             complement(346..2469)
                     /gene="scpA"
                     /locus_tag="VFP02_05745"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HET9542560.1"
                     /translation="METPDPDAWRARAEKELRGTSPDDLTWPTPEGIPVPPLYTEADL
                     TGVEHLGGLPGFPPFVRGVRATMYANRSWTLRQYGGFSTAEESNAFYQRNLAAGQTGL
                     SVAFDLATHRGYDSDHPRVEGDVGMAGVAIDSVEDTNILFADIPLDKVSVSMTMNGAV
                     LPVLACYVVSAEEQGVAQDQLSGTIQNDILKEFMVRNTYIYPPAPSMRIVADIIEHTA
                     THMPRFNSISISGYHMQEAGATADQELAFTIADGLEYVRTAHARGLDVDAFAPRLSFF
                     FAIGMNLFMEVAKLRAARLLWARVMERFDPKDEKSLMLRTHCQTSGVSLTEQDPYNNI
                     VRTAYEALAAVLGGTQSLHTNAFDEALGLPTDFSAKIARNTQLILAEETGVPHVVDPL
                     AGSYYVEKLTHDLAAKAWALIEEVEELGGMTEAVESGMPKLRIEESAARRQARVDRGD
                     EVVVGVNKYGAEHPDHVDVLDIDNTDVRRQQVERLERVRADRDDGAARAALDALTAGA
                     RGDGDLLALCIDAARARATVGEMSDAMEAVFGRHKAEIRSISGVYGAAAEGFEDFETA
                     RVAVEAFAAARGRRPRMLVVKMGQDGHDRGAKVIATAFADLGFDVDVGPLFQTPAEAA
                     RDAVENDVHVVGVSSQAAGHKTLVPQLIDELRAAGAGEVLVVCGGVIPPGDHAKLQEA
                     GVAAVFGPGTNIPKAALRVLELLDQ"
     gene            2514..>3584
                     /locus_tag="VFP02_05750"
     CDS             2514..>3584
                     /locus_tag="VFP02_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9542561.1"
                     /translation="MATRLVIIGGGPAGNAAATRAATLGAQVTLVERDIIGGAAHLWD
                     CVPSKAMIATGGAMSYLHRVEQMGLREVGAEIDLDALRERLVSIEDRLEASVTDLLDR
                     QKVRILRGTGRMTGPNTVVAETVDGDVTLPADAVLLSTGSRPRIPDWAEPDGDRVLTT
                     RQAYPPKVLPEHVAVIGSGVTGVEFVHLFASMGSKVTLVVSRQQVLPQKDPEVAAALE
                     ENFLSRDVKLLKGARATGLERGDDGVTVLCDDGRRVDCSHVLLAVGSLPNSEALGLHA
                     AGVESENGYVPVDQHLRSNVGHIYAAGDLSGKLPLSSVAAMQGRKIADHVMGLHTREH
                     RHLDYDKAASAIFTEPEIADVGL"
BASE COUNT          507 a         1254 c         1284 g          539 t
ORIGIN      
        1 atgaaggcct cgtcgcgtcg gcccagctcg cccatgcggg tcttgtcgcc caggatcgag
       61 ccccccgatc gggtgctgga ggggtcgacc gccagcacgg cgacccggtg acccgcgtcc
      121 accagatgca ggccaagggc ttcgatgaag gtcgatttcc ccgcgccggg ctggcccgtg
      181 acgccgaccc ggaccgcgcc gcccgaggcc gggaggagct cctcgacgag ggcgacggcc
      241 tccgcccggt ggtcagggcg caccgactcc acgagggtga tcccctgggc gagggcccgc
      301 cggtcaccgg agcggatcgc ctccgccgtc gtcgccatgg tgtcgttact ggtcgaggag
      361 ctcgagcacc cgcagggcgg ccttggggat gttggtcccc gggccgaaca ccgcggccac
      421 gccggcctcc tgcagcttgg cgtggtcgcc cggagggatg accccgccgc agacgacgag
      481 gacctcgccc gctcctgcgg cccgcagttc gtcgatgagc tgcgggacca gcgtcttgtg
      541 gccggcggcc tggctggaca cgccgaccac gtggacgtcg ttctcgaccg cgtcgcgggc
      601 ggcctcggcc ggcgtctgga acagggggcc gacgtcgacg tcgaagccca ggtcggcgaa
      661 ggcggtggcg atgaccttgg cgccacggtc gtggccgtcc tgacccatct tgacgaccag
      721 catccgaggg cggcggccgc gcgccgcggc aaacgcctcc acggcgaccc gcgccgtctc
      781 gaagtcctcg aagccctccg ccgctgcacc gtagacgccg gagatcgacc ggatctcggc
      841 cttgtggcgc ccgaacaccg cttccatcgc gtcggacatc tcacccaccg tggcccgagc
      901 ccgcgccgcg tcgatgcaca aggccagcag gtccccgtca ccccgagcgc ccgcggtgag
      961 ggcatcgagc gcggcgcggg cggccccatc gtcccgatcg gcccggaccc gttcgagccg
     1021 ctcgacctgc tggcgtcgga catcggtgtt gtcgatgtcg aggacatcga cgtggtcggg
     1081 atgctcggcc ccgtacttgt tcacgccgac gacgacctcg tcgccccggt caacccgggc
     1141 ctgacgtcgg gctgcgctct cttcgatgcg caacttgggc atgccggact cgacggcctc
     1201 ggtcatgccc cccagctcct cgacctcctc gatcagggcc cacgccttgg cggccaggtc
     1261 gtgggtcagc ttctcgacgt agtagctgcc ggcgagcggg tcgacgacgt ggggcacgcc
     1321 ggtctcctcg gccaggatga gctgggtgtt gcgagcgatc ttggccgaga agtcggtggg
     1381 caggcccagg gcctcgtcga aggcgttggt gtgcaggctc tgggtgccgc ccaacacggc
     1441 cgccagcgcc tcgtacgcgg tgcgcacgat gttgttgtag gggtcctgct cggtcaggga
     1501 gacgcccgag gtctggcagt gcgtgcgcag catcagggac ttctcgtcct tcgggtcgaa
     1561 gcgttccatc acccgggccc agagcaggcg ggccgctcgc agcttcgcca cctccatgaa
     1621 caggttcatg ccgatggcga agaagaacga gagccggggg gcgaaggcgt ccacgtcgag
     1681 gccccgggcg tgggccgtcc gaacgtattc gaggccgtcg gcgatggtga aggcgagttc
     1741 ctggtcggcg gtcgcccccg cctcctgcat gtggtagccg gagatggaga tcgagttgaa
     1801 gcggggcatg tgtgtcgccg tgtgctcgat gatgtcggcc acgatccgca tgctcggcgc
     1861 cggcgggtag atgtaggtgt tgcggaccat gaactccttg aggatgtcgt tctggatggt
     1921 cccgctgagc tggtcctggg ccaccccctg ctcctcggcc gacaccacgt agcaggccag
     1981 cacggggagc acggccccgt tcatggtcat ggagacactg accttgtcca gggggatgtc
     2041 ggcgaagagg atgttcgtgt cctccaccga gtcgatggcc accccggcca tgccgacgtc
     2101 gccctccacc cgagggtggt ccgagtcgta gccccggtgg gtggcgaggt cgaaggccac
     2161 cgacaggccg gtctggcccg cggcgaggtt gcgctggtag aaggcgttgg actcctcggc
     2221 ggtcgagaag ccgccgtact ggcgcagggt ccaggaccgg ttggcgtaca tcgtggcccg
     2281 gacgccgcgg acgaagggcg ggaagcccgg caggccaccg aggtgctcga cgccggtcag
     2341 atcggcctcg gtgtacagcg gcgggacggg gatgccctcg ggcgtgggcc aggtcaggtc
     2401 gtcggggctg gtgccccgca gctccttctc ggcccgggcc cgccaggcgt cgggatcagg
     2461 tgtctccatg gcagcgtcaa gggtacgtga ggtgcctgcc gtagattgag tccgtggcca
     2521 cgagactggt gatcatcggc gggggaccgg cgggcaacgc cgccgccacc cgggccgcga
     2581 ccctgggggc ccaggtcacc ctggtcgagc gggacatcat cggcggggct gcccatctgt
     2641 gggactgcgt gccctccaag gccatgatcg ccacgggcgg ggcgatgtcc tatctgcacc
     2701 gtgtcgagca gatgggcttg cgcgaggtcg gggccgagat cgacctcgac gccctccgcg
     2761 agcgcctcgt gtccatcgag gaccgcctcg aggcatcggt caccgacctc ctcgaccggc
     2821 agaaggtccg catcctccgc ggcaccggac gcatgaccgg ccccaacacc gtcgtggccg
     2881 agaccgtcga cggcgacgtg accctgccgg ccgacgccgt ccttctgagc acgggcagcc
     2941 ggccccgcat ccccgactgg gccgaacccg acggcgaccg ggtcctcacc acccgccagg
     3001 cctacccgcc gaaggtcctg cccgagcacg tggccgtgat cggttccggt gtcaccggcg
     3061 tcgagttcgt ccacctcttc gcgtcgatgg gatcgaaggt gaccctggtc gtcagccgcc
     3121 agcaggtcct cccccagaag gacccggagg tggccgccgc cctcgaggag aacttcctgt
     3181 cccgggacgt caagctgctg aagggcgccc gggccacggg cctggagcgg ggcgacgacg
     3241 gggtcaccgt gctctgcgac gacggtcgcc gggtggactg ctcccacgtc ctccttgcgg
     3301 tcggctcgct gccgaactcc gaggccctgg gcctgcacgc cgcgggggtg gagagcgaga
     3361 acggctatgt ccctgtcgac cagcacctgc gctcgaacgt cgggcacatc tatgccgcgg
     3421 gcgacctgtc ggggaagctg ccgctgtcct cggtggcggc catgcagggg cggaagatcg
     3481 ccgaccacgt catggggctc cacacccgag agcaccgcca cctggactac gacaaggccg
     3541 catcggccat cttcaccgag cccgagatcg ccgacgtggg cctg
//
LOCUS       DASTFV010000304         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1142714, whole genome shotgun sequence.
ACCESSION   DASTFV010000304 DASTFV010000000
VERSION     DASTFV010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142714"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFP02_05755"
     CDS             <1..402
                     /locus_tag="VFP02_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9542562.1"
                     /translation="GRSPDGWGQEFRGGAAYLALRCEVPVVPVHIAGTGKILGKGMKY
                     PKPARTRLTFGHPLAIEEGESSTHLNERIESAVAVLADESTTDWWEARRRVHAGTTPS
                     LTGPDAPTWRRAWALGDRKGRARQRRRWPKV"
     gene            591..>2572
                     /locus_tag="VFP02_05760"
     CDS             591..>2572
                     /locus_tag="VFP02_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HET9542563.1"
                     /translation="MREFDACGIGFVADAQGRASRAIVAAALHGLACVKHRGAAAADG
                     LTSDGCGVLTPIPAALFGEGNGVVALFVRGADATTLVEDACAAEGVEVVDWRKPETDD
                     TVLGHMAAETRPEILQAVIAPPTDSPYSDEVTAYRIRRHLTDVDGLYVVSCSYRTVVY
                     KSLAAADHLADFYRDLADPRYEAAFTIFHQRFSTNTLPTWERAQPFRSLCHNGEINAI
                     QGNRNRMLARSTLGTEEAGLGAESLFWPVLDDNTSDSGKLDEALELLTRGGRDIRHAV
                     AMLVPEAWEHAVDLDPEIRGFYRYHSALMEPWDGPAGLIFTDGVGVGAALDRNGLRPL
                     RYAICDDGLVVCSSEVGAVDVSGHGKVERGRLGPGQMLFVDPTRGVQRNDAVKERLAA
                     ANTFSHWAHDGFLSFGPGEPVMQVDEGLEARQAVHGYTQEELQMVLRPMGAEGKEPTF
                     SMGDDSPLPPMAGRPRPVHHYLKQRFAQVTNPPIDPLREHRVMSLRTLLGPRVPILSE
                     HEDATRLLRLESFLLYPDAVEQLAEGQTPFRAATLDATFPLTDGADGLRAGVARLADE
                     VEAVTADGAEILVVTHAGADADRVPIPSVLAVGAAHHRLIELRRRSDVSVVVLADDAR
                     DVHFFAALVGYGADAVCPRLALETLADWADNSDDDTT"
BASE COUNT          368 a         1020 c          840 g          344 t
ORIGIN      
        1 ggccgctccc ccgacggctg gggccaggag ttccggggcg gcgccgccta cctcgctctc
       61 cgctgcgagg tgcccgtcgt ccctgtccac atcgccggca ccggcaagat cctgggcaag
      121 ggcatgaagt acccgaagcc cgcccgtacc cgcctcacct tcggtcaccc cctcgcgatc
      181 gaggaggggg agtccagcac ccacctgaac gaacgcatcg agtccgccgt cgccgtcctg
      241 gccgacgagt ccaccaccga ctggtgggag gcccgtcggc gggtccacgc cggcaccacg
      301 ccctcgctta ccggccccga cgcccccacc tggcgtcggg cctgggccct cggcgaccgc
      361 aagggccgcg cccgccagcg ccgccggtgg cccaaggtct gacccgccat ccgggtggaa
      421 ttcggttggg aaggttcaga tcgccctcag ctacactccc ctgacgccct cccgggccca
      481 ggcaacggcg ccgcgctcct gtcaccgccg actttcgcgc cccgggaact cgggcctgtc
      541 gacgcaaccc gggtcgttca cgcgacccct caacaggagg agtgccctcg atgcgggagt
      601 tcgacgcgtg tggcatcggc ttcgtggccg atgcgcaggg tcgggcctcg cgcgccatcg
      661 tggccgccgc actgcacggg ctggcgtgcg tcaagcaccg gggggccgcc gccgccgacg
      721 gactgacctc ggacggctgc ggcgtcctca cgcccatccc cgcggctctg ttcggggaag
      781 gcaacggggt cgtcgccctc ttcgtgcggg gtgccgacgc cacgaccctg gtcgaagacg
      841 cctgcgcggc cgagggcgtc gaggtggtcg actggcgcaa gcccgagacc gacgacaccg
      901 tcctcggcca catggcggcc gagacccggc ccgagatcct ccaagcggtc atcgctccgc
      961 cgacggacag tccctacagc gacgaggtca cggcctaccg gatccgccgc cacctgaccg
     1021 atgtcgacgg cctctacgtc gtgtcctgct cctaccggac cgtcgtctac aagagcctgg
     1081 ccgccgccga ccacctggcc gacttctacc gggacctcgc cgacccccgg tacgaagccg
     1141 ccttcaccat cttccaccag cggttctcga ccaacacgct gcccacctgg gaacgggccc
     1201 agcccttccg cagcctgtgc cacaacggcg agatcaacgc catccaaggc aaccgcaacc
     1261 gcatgctggc ccggtccacc ctgggcaccg aagaggccgg cctcggagcc gaatccctgt
     1321 tctggccggt cctcgacgac aacacgtcgg actcgggcaa gttggacgag gccctcgagc
     1381 tcctcacccg gggcgggcgg gacatccgcc acgccgtggc catgctcgtg cccgaggcct
     1441 gggagcacgc cgtcgacctc gatcccgaga tccgtggctt ctaccgctac cactccgcgc
     1501 tcatggagcc ctgggacggc cccgccggcc tgatcttcac cgacggcgtc ggggtcggcg
     1561 ccgccctcga ccgcaacggc ctccgcccgc tgcgctacgc catctgcgac gacggcctcg
     1621 tggtgtgctc gtccgaggtc ggggcggtgg acgtcagcgg ccacggcaag gtcgagcggg
     1681 gccgcctcgg ccccggccag atgctcttcg tcgatcccac ccggggcgta cagcgcaacg
     1741 acgccgtgaa ggagcgcctg gccgcagcca acaccttctc gcactgggcc catgacgggt
     1801 tcctgagctt cgggccgggc gagccggtga tgcaggtcga cgaggggctc gaagcccgtc
     1861 aggccgtcca cggctacacc caggaggagc tgcagatggt cctccggccg atgggtgcgg
     1921 agggcaagga acccaccttc tcgatgggcg acgactcgcc cctccccccc atggccggcc
     1981 ggccccgtcc ggtccaccac tacctgaagc agcgcttcgc ccaagtcacc aacccgccga
     2041 tcgacccgct gcgcgagcac cgggtgatgt ccctgcgcac gctgctggga ccccgggtcc
     2101 ccatcctcag cgagcacgag gacgccacgc gcctgctgcg cctcgaatcc ttcctgctct
     2161 accccgacgc cgtcgagcag ctggccgaag gccagacccc cttccgggcc gcgacgctgg
     2221 acgccacgtt ccccctgacc gacggggccg acggcctgcg ggccggggtc gcccggctgg
     2281 ccgacgaggt cgaggcggtc accgccgacg gggccgagat cctcgtcgtc acccacgccg
     2341 gagccgacgc cgaccgggtc ccgatcccgt ccgtgctcgc ggtgggcgcg gcccaccacc
     2401 ggttgatcga gctcaggcgg cggagcgacg tgagtgtggt ggtcctggcc gacgacgccc
     2461 gggacgtgca cttcttcgcc gcgctcgtcg gctacggcgc cgacgcggtg tgtccccgcc
     2521 tggcgctgga gaccctggcc gactgggccg acaacagcga cgacgacacc ac
//
LOCUS       DASTFV010000305         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1145668, whole genome shotgun sequence.
ACCESSION   DASTFV010000305 DASTFV010000000
VERSION     DASTFV010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145668"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /locus_tag="VFP02_05765"
     CDS             <1..1536
                     /locus_tag="VFP02_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HET9542564.1"
                     /translation="IALQEEVGWISPGATNHVGRQLAVPPAEIYGVATFYELLRTTEP
                     DGDGPTTWVCVDAACRIRSDAAPVADGAVRHDAPCLGRCEQAPAAFVQRRGPAEAAPE
                     PVPFTLPQAGDPDLRLLRRVGRVDPTSLDSYLAHGGGEALVRARELGPDAVIARLTDA
                     GLSGRGGAAFPTGVKWRAVRDAAGERKHVVVNGDESEPGTFKDRTLLEQDPFALVEAT
                     VIAAETVGAGEAWVYIRGEYPEATSRVEAAIAQVDAAGWLRDGLRIEVRRGGGAYICG
                     EETALFNSIEGFRGEPRNKPPFPTTNGLFDQPTAVNNPETLLNVLPIVLEGPEAYRAV
                     GTEQSPGTRLFCLSGRIGRPGVYEAPFGITLGDLLDLAGGVEGELAAVLLGGAAGMFV
                     GPDSLDLALTLEDTRAAGATLGSGVVMVFDTAVDLTDVVVRIAEFFRSESCGQCVPCR
                     VGTVRVHETMVQMRERGGLTDERRQLLDEMATAMTDASICGLGHTAVGAVRSALDLGL
                     VGS"
     gene            1533..2375
                     /locus_tag="VFP02_05770"
     CDS             1533..2375
                     /locus_tag="VFP02_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9542565.1"
                     /translation="MIRLTVDGHELEVDEGTTILGACTEAAIDTPTICWAPNLTPVNV
                     CRVCVVEVEGSRALVPACSRPVEDGMVVHTDSDRVRHSRKIVLEFLGSGVDLSQADGL
                     GRWIEHYDADPSRYGTQDHPVERVEEAVKVQDGLYIRDYEKCVLCYKCVEACGDDAQH
                     TYAIAVSGRGFDARISTEYDVELPDSACVYCGNCIGVCPTNALQFKSEFDLRNAEDWR
                     PEEQTVTTTVCSYCGVGCNLELHVQDERIVKVTSPVDHEVTSGHLCIKGRFGWEYVQG
                     PPKG"
     gene            2441..>2809
                     /locus_tag="VFP02_05775"
     CDS             2441..>2809
                     /locus_tag="VFP02_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542566.1"
                     /translation="MSLRRRGVGILGVALVLGLALAACGGSGYEYVSNDEAGIFFKVP
                     DDWAVIDVDTDDGTLGRPGVPLDWVRVVDSSPVPTVTNYRTPVPTDPVGIAVVEPVET
                     ASQRDGLSLEMLRTYALSGYV"
BASE COUNT          396 a         1004 c         1008 g          401 t
ORIGIN      
        1 atcgccctcc aagaggaggt gggctggatc agcccgggcg ccacgaacca cgtcggccgc
       61 cagctcgccg tgcccccggc cgagatctac ggggtggcca cgttctacga gctgctgcgc
      121 acgaccgagc ccgacggcga cgggccgacg acctgggtct gcgtcgacgc ggcctgccga
      181 atcaggtcgg acgccgcgcc ggtggccgac ggggcggtgc ggcacgacgc gccctgcctc
      241 ggtcgctgtg agcaggcgcc cgccgcgttc gtccagcgcc ggggacccgc ggaggccgcg
      301 ccggagccgg ttccgttcac cctgccccag gccggcgatc ccgacctgcg cctgctccgt
      361 cgggtcggcc gggtcgatcc gaccagcctc gactcctacc tcgcccacgg cgggggcgag
      421 gcgctggtcc gcgcccgcga gctgggtccg gacgccgtca tcgcgcggct gaccgacgcc
      481 ggcctgtcgg gtcggggcgg tgccgcgttc ccaaccggcg tgaagtggcg ggcggtacgg
      541 gacgcggcgg gcgaacgcaa gcacgtcgtg gtcaacggcg acgagtccga gccgggcacc
      601 ttcaaggacc ggaccctgct cgagcaggac ccgttcgccc tggtcgaggc cacggtgatc
      661 gccgccgaga cggtgggggc gggcgaggcc tgggtctaca tccgcgggga gtaccccgag
      721 gcgacgagcc gggtcgaagc cgccatcgcc caggtcgacg cggccggctg gctgcgggac
      781 ggcctccgca tcgaggtccg gcgaggcggt ggggcctaca tctgcggcga ggagaccgcc
      841 ctcttcaact ccatcgaggg cttccggggc gagccccgca acaagccccc cttccccacg
      901 acgaacggcc tcttcgacca accgaccgcc gtcaacaacc cggagaccct cctgaacgtc
      961 ctgcccatcg tcctggaggg acccgaggcc taccgcgccg tgggcaccga gcagtcgccg
     1021 ggcacccgcc tgttctgcct gtcgggcagg atcgggcgtc ccggggtcta cgaggccccg
     1081 ttcggcatca ccttggggga cctgctcgac ctcgccggcg gcgtcgaggg cgagctggcg
     1141 gccgtcctcc tcggcggcgc ggccgggatg ttcgtcgggc ccgactccct cgacctggcg
     1201 ctcaccctgg aggacacgcg ggcggcgggg gccaccctcg gctcgggagt ggtgatggtc
     1261 ttcgacacgg cggtcgacct gaccgatgtc gtcgtgcgca tcgccgagtt ctttcgcagc
     1321 gagtcctgtg gccagtgcgt gccgtgccgc gtcggtacgg tgcgcgtcca cgagacgatg
     1381 gtgcagatgc gggaacgtgg tggcttgacc gacgaacggc gacagctgct ggatgagatg
     1441 gcgacggcca tgaccgacgc ctccatctgc ggcctgggcc acaccgccgt cggcgccgtc
     1501 cgctcggccc tggacctggg gctggtggga tcatgatccg gctcaccgtc gacggccacg
     1561 agctggaggt cgacgagggc acgacgatcc tcggggcctg caccgaggcg gccatcgaca
     1621 cgccgaccat ctgctgggcg cccaacctga ccccggtcaa cgtgtgccgg gtctgcgtgg
     1681 tcgaggtcga ggggtcccgg gcgctggtgc cggcctgctc ccgccccgtc gaggacggca
     1741 tggtcgtcca cacggattcg gaccgggtcc gtcacagccg caagatcgtc ctggagttcc
     1801 tcggctcggg ggtcgacctg tcccaggccg acgggctggg gcgctggatc gagcactacg
     1861 acgccgaccc gtcccggtac ggcacccagg accatccggt cgagcgggtc gaggaagcgg
     1921 tcaaggtcca ggacgggctc tacatccggg actacgagaa gtgcgtgctc tgctacaagt
     1981 gcgtcgaggc ctgcggcgac gacgcccagc acacctacgc catcgccgtc agcggccggg
     2041 gcttcgacgc ccgcatctcc accgagtacg acgtcgagct ccccgactcg gcctgcgtct
     2101 actgcggcaa ctgcatcggc gtctgcccca ccaacgcgct gcagttcaag agcgagttcg
     2161 acctgcggaa cgccgaggac tggcgcccgg aggagcagac cgtcaccacg accgtgtgct
     2221 cctactgcgg cgtcggttgc aacctggagc tgcacgtcca ggacgagcgc atcgtcaagg
     2281 tcaccagtcc cgtcgaccac gaggtcacca gcggccacct ctgcatcaag ggccgcttcg
     2341 gctgggagta cgtccaaggc ccgcccaagg gttgacccac cgtcgagcgg attcggtcgc
     2401 gactgtcgcc gtcgggcgat taccggcaca ctggacatcc atgtcgctca ggcggagggg
     2461 tgtggggatc ctcggggtcg cgctcgtcct cggcctggcc ctggccgcct gtgggggctc
     2521 gggttacgag tacgtgtcca acgacgaggc ggggatcttc ttcaaggtgc ccgacgactg
     2581 ggcggtgatc gacgtcgaca ccgacgacgg cacgctcggg cgtccggggg tgcccttgga
     2641 ctgggtccgg gtggtggaca gctcgccggt gccgaccgtg accaactacc gcacgccggt
     2701 accgaccgat ccggtcggca tcgccgtcgt ggagccggtg gagaccgcca gtcagcgcga
     2761 cggcctcagc ctggagatgc tccgcaccta cgccctgtcc ggctacgtg
//
LOCUS       DASTFV010000306         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1147782, whole genome shotgun sequence.
ACCESSION   DASTFV010000306 DASTFV010000000
VERSION     DASTFV010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147782"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VFP02_05780"
     CDS             <1..722
                     /locus_tag="VFP02_05780"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HET9542567.1"
                     /translation="EVGQYAVDTMTPIGAGTYEAARAAVDVTLTATDLVLGGAPAAYA
                     AVRPPGHHVGPGFYGGSCHLNNAACAAQYLVDRGGARVAIVDIDAHHGNGTQEVFWER
                     GDVLFGSVHVDPGAGWLPHTAGFADEVGEGRGAGANRNLPLPPGSGDGPWGEAVRVLA
                     TAAADHGAEARVVSLGVDAAAGDPESPLEVSRRGFAEAGRSLAALGLPTVFVQEGGYD
                     LNTLGQLVLAVLDGFEEARDC"
     gene            712..>2048
                     /locus_tag="VFP02_05785"
     CDS             712..>2048
                     /locus_tag="VFP02_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542568.1"
                     /translation="MTADRPTWLGTEERGGLPIPPRVDTVPPPHWRLEAVAATERPRD
                     LTLGPDGRTAAFIWDRDTSDVWVIDALTGATSRLTTGRALAPWWEDTRPVVSPDGALV
                     AYGDGDQVWVVPVAGGPPRAVLEGGEPVWTPDGLLLIGVERDRCTVLAQVDVDDPWPV
                     PVASGDGDCWGADPSPDGTRVAYVFSPHADRNRSEIHVVDLDSGEDRALTGTPEHHDR
                     GVTWSPDGATLAFTAERPGWHEVHLIGADGTGERQLTDGGHNWFDLAWHPDGDRLVGV
                     RDRHGVTDLVVVDVEGGALDVLAEGGSWSQPQWLADGRVLVAHESFTTAPRLQVVEPG
                     GVTTTVLAPTPAAVASAPHVVPEPVSSVSFDGREIPGWLYLPAGASAEQPVPAVVNPH
                     GGPTDRDGDEWHGVAQYFCDKGYAWFLPNFRGSTSWGRDHEHANHGDWGVGDT"
BASE COUNT          259 a          745 c          779 g          265 t
ORIGIN      
        1 ccgaggtcgg ccagtacgcc gtcgacacga tgacgccgat cggcgccggg acctacgagg
       61 cggcgcgagc cgcggtggac gtaaccctga ccgcgacgga cctcgtcctg ggcggggccc
      121 cggcggccta cgccgcggtc cggccaccgg gccaccatgt gggtcccggc ttctacggcg
      181 gctcctgtca cctgaacaac gccgcctgcg ccgcccagta cctggtcgac cggggcgggg
      241 cccgcgtggc gatcgtggac atcgacgccc atcacggcaa cggcacccag gaggtcttct
      301 gggagcgggg cgacgtcttg ttcggctcgg tccacgtgga cccaggcgcg ggctggttgc
      361 cccacacggc gggcttcgcc gacgaagtcg gcgaaggaag gggcgcgggg gcgaaccgca
      421 acctgccgtt gccaccgggc tcgggtgacg gtccgtgggg tgaggcggtg cgggtgctgg
      481 cgaccgcggc ggccgaccac ggcgccgagg cccgggtggt cagcctcggg gtggacgccg
      541 cggcgggcga ccccgagagc ccgctggagg tcagtcgccg gggctttgcc gaggcgggcc
      601 gtagcctcgc cgccctcggc ctcccgacgg tgttcgtgca ggagggcggc tatgacctga
      661 acacgctcgg ccagctcgtg ctcgccgtgc tcgacggctt cgaggaggcc cgtgactgct
      721 gaccgtccga cctggctggg gaccgaggag cggggcggcc tgccgatccc gccccgagtc
      781 gacactgtcc cgccgccgca ctggcgcctc gaggcggtgg ccgccaccga gcggccgcgg
      841 gacctgaccc ttggccccga cggccggacc gcggccttca tctgggaccg ggacacctcc
      901 gacgtgtggg tgatcgatgc cctgacgggg gccaccagcc ggctgacgac gggccgggcg
      961 ctggccccgt ggtgggagga cacccggccc gtggtgtcac ccgacggcgc cctcgtcgcc
     1021 tacggggacg gcgatcaggt ctgggtcgtc cctgtggccg gcgggccgcc ccgggccgtc
     1081 ctcgaagggg gcgagccggt gtggacgccg gacggcctgc tcctgatcgg cgtcgagcga
     1141 gaccgctgca cggtcctggc ccaggtcgac gtggacgatc cctggcccgt cccggtggcc
     1201 tcgggagacg gtgactgttg gggggccgac ccgtcgccgg acggcacgcg tgtggcctat
     1261 gtgttctcgc cccacgccga ccgcaaccgg tcagagatcc acgtggtgga cctcgactcg
     1321 ggcgaggacc gggcgctcac cggcacaccc gagcaccacg accggggcgt gacctggtca
     1381 cccgacggcg ccaccttggc cttcaccgcc gagcgccccg gctggcacga ggtccacctc
     1441 atcggggccg acggcaccgg cgagcgtcaa ctcaccgacg ggggtcacaa ctggttcgac
     1501 ctggcgtggc acccggacgg ggaccggctc gtcggcgtgc gggaccgtca tggcgtgacc
     1561 gacctcgtcg tggtggacgt cgagggaggg gcactcgacg tgctggccga gggaggttcg
     1621 tggagccaac cccagtggct ggccgacggc cgagtcctcg tcgcccacga gtccttcacc
     1681 acggcgcccc gcctgcaggt ggtcgagccg ggcggggtca ccaccacggt cctggccccg
     1741 accccggcgg cggtcgcctc ggcccctcat gtcgtcccgg agccggtgag ctccgtgtcc
     1801 tttgacggac gcgagatccc cgggtggctc tacctgccgg ccggcgcgtc ggccgagcag
     1861 ccggtgccgg cggtggtcaa cccgcacggc gggcccaccg accgcgacgg cgacgagtgg
     1921 cacggcgtgg cccagtactt ctgtgacaag ggctacgcgt ggttcctgcc caacttccgg
     1981 gggagcacct cgtggggccg ggaccacgag cacgccaacc acggcgactg gggcgtcggc
     2041 gacaccaa
//
LOCUS       DASTFV010000307         3142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1149227, whole genome shotgun sequence.
ACCESSION   DASTFV010000307 DASTFV010000000
VERSION     DASTFV010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149227"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2009
                     /locus_tag="VFP02_05790"
     CDS             <1..2009
                     /locus_tag="VFP02_05790"
                     /inference="COORDINATES: protein motif:HMM:NF016850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HET9542569.1"
                     /translation="SAFERFDLFDKRVRFLQGSYGGTLASAPIEKVALLRIGSSAREQ
                     ADEILERLYDKVTVGGIVVVDGYDDPACRRRVDAFREKRGIADQLVRGDHWGAHWRKL
                     GEDGYEPPEPEVDDLEMPEDLTRDLPEDVALPEPGDERAVGTTGKDLSVVVVFYNMKR
                     EAERSLRSLSRAYQEEIEDLDYEVLVIENGSRPDQRLGAEYVRSFGPEFRYIDMGEGA
                     PSSPVPALNRGIAEATGENLALMIDGAHVLTPGVLHFGMEGLRTYEPAIVATQQWYVG
                     PGQQGELMAAGYDQTMEDRLFDRIGWPAEGHRLFDIGHFIGERDWFDGLWESNCLFTP
                     RRLVEQSGGFDEIFDMPGGGFANLDIYERLGSAPGVTVATILGEGSFHQFHHGTTTNQ
                     DEVENRHRLISSYAEHYEAERGKVFAASGKPIHYVGRLFEGATRTRSRRFVARAFWTP
                     QSTEGRDGVPDEPQPMPQEIAMAFTEAFWHSLAWKETTWLGEAVPAAPTDLVVYQELL
                     SRVRPDWVVITSGGGAGIASFVASICDLLDHGRVLVVGDDGVRSHPRVERVGGRPADD
                     ATRQAVAERVGDGSAFVVLGGPKHRDHTIGEFKAYAPLVPVGSYVVVEQSVVNGAPVW
                     PGFGPGPQEAIRLIKQDHPEFMSDLEVQRYALTLNANGFLKRVR"
     gene            complement(1993..2967)
                     /locus_tag="VFP02_05795"
     CDS             complement(1993..2967)
                     /locus_tag="VFP02_05795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET9542570.1"
                     /translation="MTGGERPLSVVVVTYDMERELPRTLRSLSADLQVGIEASDYEVI
                     VVDNGSPRPVDPGRFADLVGTYRFERIDPAPPSPARAANLGIGLATGETIGLIVDGAR
                     IASPGLLHMALQARRLDDRPVIASLGWHLGTARHMEAAESGFDQAAEDDLLARSGWEE
                     DGYRLFGISTLGGSSAQGWFLPISESSALFLPAALWRELGGLDEAFVLPGGGLVNHDL
                     YRRAVELDGTRLVTLLGEGTFHQYHGGAATSGRHGWDEMDADYQRLRGTPYRKPAAPS
                     LYVGTMPPAALGHLDHSSRAVRAKLVRASARRAWWRRLARRLRLSAPA"
BASE COUNT          450 a         1124 c         1129 g          439 t
ORIGIN      
        1 gcagcgcctt cgagcgtttc gacctgttcg acaagcgggt gcgcttcctc cagggctcct
       61 acggcgggac gctggcctcg gccccgatcg agaaggtggc cctgctccgg atcggttcca
      121 gcgcccgcga gcaggccgac gagatcctcg aacggctcta cgacaaggtc acggtgggcg
      181 ggatcgtggt cgtcgacggc tacgacgacc cggcgtgccg caggcgggtg gacgcgttcc
      241 gggagaagcg cgggatcgcc gaccagctgg tgcggggcga ccactggggg gcccactggc
      301 gcaagctggg cgaggacggc tacgagcccc cggagcccga ggtcgacgac ctcgagatgc
      361 ccgaggacct cacccgtgac ctcccggagg acgtggcgct ccccgagccc ggcgacgagc
      421 gggccgtggg caccaccggc aaggacctgt cggtcgtcgt cgtcttctac aacatgaagc
      481 gagaggccga gcgctccctc cggtcgctga gccgcgccta ccaggaggag atcgaggacc
      541 tcgactacga ggtcctggtc atcgagaacg gctcccggcc tgaccagcgc ctgggggcgg
      601 agtacgtccg cagcttcggt cccgagttcc gttacatcga catgggcgag ggcgccccga
      661 gctcgccggt gcccgcgctg aaccggggga tcgccgaagc caccggcgag aacctcgccc
      721 tcatgatcga cggggcccac gtgctgacgc ctggggtgtt gcacttcggc atggagggcc
      781 tgcgcaccta cgagccggcg atcgtggcca cccagcagtg gtacgtcggc cccggccagc
      841 agggcgagct gatggcggcg ggctacgacc agaccatgga ggaccggctc ttcgaccgca
      901 tcggttggcc cgccgagggc caccggctgt tcgacatcgg ccacttcatc ggcgagcgtg
      961 actggttcga cgggctctgg gagagcaact gcctgttcac cccgcggcga ctcgtcgagc
     1021 agtcgggcgg cttcgacgag atcttcgaca tgcccggcgg cggcttcgcc aacctcgaca
     1081 tctacgagcg cctcgggtcc gcccccggcg tcaccgtggc caccatcctc ggcgagggct
     1141 cgttccacca gttccaccac gggacgacga ccaaccagga cgaggtcgag aaccgccacc
     1201 ggctcatcag ctcctacgcc gagcactacg aggccgagcg gggcaaggtc ttcgccgcct
     1261 ccggcaagcc catccactac gtgggccgcc tcttcgaggg cgccacccgg acccggtccc
     1321 gccgcttcgt ggcccgtgcg ttctggacgc cccagtcgac cgagggccgc gacggcgtgc
     1381 ccgacgagcc ccagccgatg ccccaggaga tcgccatggc cttcaccgag gccttctggc
     1441 acagcctcgc ctggaaggag acgacctggc tgggcgaagc ggtgcccgcc gcgcccaccg
     1501 acctcgtcgt ctaccaggag ctcctgagcc gggtgcggcc cgactgggtg gtgatcacca
     1561 gcggcggtgg cgccggcatc gcctcgttcg tggcgtcgat ctgcgatctg ctcgaccacg
     1621 gccgcgtgct cgtggtcggc gacgatggcg tccggtcgca tccccgcgtc gagcgcgtcg
     1681 gcggccggcc cgccgacgac gccacccgcc aagcggtcgc cgagcgcgtc ggcgacggct
     1741 cggccttcgt ggtcctcggc ggccccaagc accgcgacca cacgatcggc gagttcaagg
     1801 cgtacgcgcc gctcgtgccc gtcgggtcct acgtggtggt ggagcagtcg gtggtcaacg
     1861 gcgcgccggt ctggcctggc ttcgggcccg gtccccagga ggcgatccgc ctgatcaagc
     1921 aggaccaccc ggagttcatg tcggacctcg aggtccagcg ttacgcgctg acgctcaacg
     1981 ccaacggctt cctcaagcgg gtgcgctgag ccggagccga cgggcgaggc ggcgccacca
     2041 ggctcggcgg gccgacgccc gcaccagctt ggcgcggacc gccctcgacg agtgatcgag
     2101 gtggccgagc gcggcgggcg gcatcgtgcc gacgtagagg gacggcgcgg ccggcttccg
     2161 gtagggggtg ccccggagcc gctggtagtc ggcgtccatc tcgtcccagc cgtggcgtcc
     2221 cgacgtggcc gcgcccccgt ggtactggtg gaaggtcccc tcgccgagca gcgtcaccag
     2281 ccgcgtgccg tcgagctcca cggctcggcg gtagaggtcg tggttgacga ggcccccgcc
     2341 cggaaggacg aacgcctcgt cgaggccgcc cagctcccgc cacagcgccg ccggcaggaa
     2401 cagggcgctg ctctcggaga tcgggaggaa ccagccctgt gccgacgagc cgccaagggt
     2461 gctgatgccg aagaggcggt agccgtcctc ctcccagccg gaccgggcca ggaggtcgtc
     2521 ctcggcggcc tggtcgaagc cggactcggc ggcctccatg tggcgggcgg tgccgaggtg
     2581 ccacccgagg ctggcgatca ccggccggtc gtccaaccgc cgggcctgca gggccatgtg
     2641 gaggaggccg ggcgaggcga tccgggcacc gtcgacgatg aggccgatcg tttcccccgt
     2701 cgccaggccg atgccgaggt tcgcggcccg ggccggggac ggcggggccg ggtcgatccg
     2761 ctcgaagcgg tacgtgccca ccaggtcggc gaaccggccg gggtcgacgg gccggggtga
     2821 gccgttgtcg accacgatga cctcgtagtc ggacgcctcg atgccgacct ggagatcggc
     2881 cgacaaggag cgcagcgtgc gcggcagctc ccgttccatg tcgtaggtca cgacgaccac
     2941 gctgagcggc cgctctccgc cggtcatctt gatggcttca gtccgtcttc gccttgccgg
     3001 cagccttctt tgccttcttc caggccctga cgtccaagag ctcggcctca gagccgatgt
     3061 cggccacgga gcggggttga tcgtcggcaa acgggccggc cacggcttcc cagccagcgg
     3121 gggctcggtt gaagcgcttg gc
//
LOCUS       DASTFV010000308         4408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1152704, whole genome shotgun sequence.
ACCESSION   DASTFV010000308 DASTFV010000000
VERSION     DASTFV010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152704"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1029
                     /locus_tag="VFP02_05800"
     CDS             124..1029
                     /locus_tag="VFP02_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9542571.1"
                     /translation="MDFLEQRLVLQAAFTDRIIGQVMDRLREADLYDEAIVIATADHG
                     IGLEPGGPARVLTSADVPTENLADTYYVPLVIKAPGLGPAGTVSDANVGTIDIVPTIV
                     AALGFQLPWPVDGIDLGSRERPDAEKRVGVVTGGIGAGTPTLSGEVTFDGETQLAVML
                     SKNIDTLFRSDNPAYRPFAVSDAGEIVGRRPEELTITSPSGASAVVHGLDAFEQVDLA
                     SGSLPAYLVADLDVAGTAEPTVAVVLNGRVAAVSPSWSSGGSPHHLEAMLVPDFFRQG
                     ANQLEIYLVGGPEGERTLTPVEVTP"
     gene            complement(1046..3385)
                     /locus_tag="VFP02_05805"
     CDS             complement(1046..3385)
                     /locus_tag="VFP02_05805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9542572.1"
                     /translation="MTVTPRVSVVVPVYGGAAETGRCLASVLDHAPALTVPFDLVVVD
                     DASPDPAVRAELERVAAEPAPVAVTLLRNPDNVGFVASVNRGIRHADGDVVVLNADTV
                     VTAGWLDALADAARGEDVATVTPLTSSGSFCTLPPPVVAEFGLDGPEPRIDACADFVR
                     EQSLGLRPEVITGVGFCLYVTREAIDRCGPLDERDFGRGYGEEVDFCLRATRLGFRHL
                     VEDATYVHHWGGVSFGAERVAGLARGSAVLRERYPFFRATNARERAEHPLAVAFKALE
                     LALTERNPARPHVLQVLHQPSPAAGGTEKHVQALDEALGDRFDVSVLHPVESGFVLRT
                     RWMVGGPEPIAHELLFPGGGARARGVHDPVAAAALQTVLDLFDVDAVHLHNLIGHSLA
                     PLSVLADFPGPVVCTVHDLYLACPNHSLLYLDTEPCGIPEDLEVCARCLPASRVGEPV
                     SYLRRFRAEVEAHLDVVDHFVVASQSAADHLLRAYDVDEARIELIEHGAIIEVPDERV
                     VDERHVLEDPLRLAFVGRGWRKKGIDVASGLADAGVASGVEVHHFGPLEEPASPSLVR
                     HGAYDNRELPRLLRDAGIHVVLLPGPYAETFGYVMTEALAAGLPVIGAGYGALGQRIR
                     ALGVGWTFDPQQEDELPSLVEHLDRCRPEVLRATRRAAATELRSTAATADRYAELYQG
                     AHLPTDRRRPTVTDHADAEADRLRRHARALAAVNHQLHAQLDATARTRGRDLTRRWYD
                     ELERRAPRTQRSLELVLEAAASARLLVDMASAAVRRRRG"
     gene            complement(3382..4224)
                     /locus_tag="VFP02_05810"
     CDS             complement(3382..4224)
                     /locus_tag="VFP02_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542573.1"
                     /translation="MAPADPAAGPTLVLPDGWCLDWISIDRLERALAAAPSTVAGVGA
                     ASGPLRPGGSYRTHAEWRALRPAHLEPVAGPVAGAVLVREDVEHEVDGGQVHVAGPVT
                     LDPGAHVHDPLADPGPIEVAHGEGRSPFPVRPVVVFLAVEAGGGEDRWALRLVNDLLT
                     EDVEARLAAGEVAEGPQLTRPCRPDPASIAALRPQVVVALDAGAAAVASPWCGSDRRT
                     VVVEVDPVLGREPALVDWRIGRASGRLRARIGTSVGAATFAELVNRLCSGPHPVPPRQ
                     DVPG"
     gene            complement(4224..>4408)
                     /locus_tag="VFP02_05815"
     CDS             complement(4224..>4408)
                     /locus_tag="VFP02_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542574.1"
                     /translation="IRRGWLRAPADTRQLAEARAQVVALTEEIRARNRQRDAQLGTRV
                     RRRVRSVRRRMEGTTG"
BASE COUNT          620 a         1598 c         1639 g          551 t
ORIGIN      
        1 cacctcgtgc tcccgcacgg tccctacgac ttcctgcccg acggccgccg ctacacaggc
       61 gggccgggcc cccaggccga aggtctccgg gacatcgtcg gccggtcgga ccaggtcgcc
      121 gaggtggact tcctggagca acgcctggtg ctgcaggccg ccttcaccga tcggatcatc
      181 ggtcaggtga tggatcgtct gcgagaggcg gacctgtacg acgaggccat cgtcatcgcc
      241 acggcggacc atggcatcgg actcgaaccc ggtgggcccg cccgggtcct gacgagcgct
      301 gacgttccga cggagaacct ggccgacacg tactacgtgc cgctggtgat caaggccccg
      361 ggcctcgggc cggccggcac cgtgagcgat gccaacgtcg ggacgatcga catcgtcccg
      421 acgatcgttg ccgcactcgg cttccagctg ccctggccgg tggacggcat cgacctgggt
      481 tcacgggaac gcccggatgc tgagaagcga gtcggcgtcg tcaccggcgg catcggagcg
      541 gggacaccca cgctcagtgg cgaagtcacc tttgacggcg aaacgcagct cgccgtgatg
      601 ctcagcaaga acatcgacac cctgttccgg agtgacaatc ccgcctatcg ccccttcgcc
      661 gtatcggatg cgggggagat cgtgggccga cggcctgagg agctgaccat caccagcccg
      721 tccggtgcca gtgcggtggt tcatggactg gacgccttcg agcaggtgga cctggcgagc
      781 gggagcctcc ctgcctactt ggtggccgac ctcgatgtcg cgggcacggc ggaacccacc
      841 gtcgcggtcg tgctcaacgg gcgggtcgcg gcggtgagcc cgagctggtc atcaggcggc
      901 agtccccatc acctcgaagc gatgctggtc cccgacttct tccgccaggg tgcgaatcag
      961 ctcgagatct atctggtggg cggccccgag ggagagcgca cgctcacgcc ggttgaggtg
     1021 acaccgtgac gtcgcccgtc gggtgtcagc cccgacggcg acggaccgct gcgctggcca
     1081 tgtcgacgag cagccgcgcg gaggccgcgg cctccagtac cagctcgagg ctccgctggg
     1141 tccgcggggc gcggcgctcg agctcgtcgt accaacgccg agtcaggtcg cggccccgcg
     1201 tccttgccgt cgcgtcgagc tgggcgtgca gctgatggtt caccgccgcc agcgcgcggg
     1261 cgtgccgtct gagtcgatcc gcctcggcgt cagcatggtc ggtcacggtg ggccgccttc
     1321 ggtccgtcgg caggtgtgct ccctggtaca actcggcata gcggtcagcg gtggcggcgg
     1381 tcgatcggag ctccgtcgcc gcggctcgac gggtggcccg caggacctcc ggtcggcaac
     1441 ggtcgaggtg ctccaccagg gacggtagtt cgtcctcctg ctgggggtcg aacgtccagc
     1501 ccacccccag cgcccggatc cgctggccga gcgcgccgta gccggcaccg atcaccggca
     1561 gcccggcggc cagcgcctcg gtcatcacgt agccgaaggt ctcggcgtag gggcccggca
     1621 gcagcacgac gtggatgccg gcgtcccgga ggagtcgagg cagctcgcgg ttgtcgtagg
     1681 cgccgtgccg aaccaggctg ggcgacgccg gctcctcgag cggtccgaag tgatggacct
     1741 cgacgccgga ggccacaccg gcgtcggcaa ggccgctggc gacgtcgatg cccttcttgc
     1801 gccagccccg gcccacgaac gccagcctga gggggtcctc cagcacgtgg cgctcgtcga
     1861 ccacccgctc gtccgggacc tcgatgatcg ccccgtgctc gatgagctcg atccgggcct
     1921 cgtcgacgtc gtaggcccgc agcaggtggt cggccgcgct ctgactggcg acgacgaagt
     1981 ggtcgacgac gtcgaggtgc gcctcgacct ccgcccggaa gcgacggagg taggacacgg
     2041 gctcgccgac gcggctcgcc ggcaggcagc gggcacacac ctcgaggtcc tcggggatgc
     2101 cgcagggctc ggtgtcgagg tagaggagcg agtggttggg gcaggccagg tagaggtcgt
     2161 gcacggtgca gacgaccggc ccggggaaat cggccagcac cgagagcggg gcgagcgagt
     2221 ggccgatgag gttgtgcagg tgcacggcgt cgacgtcgaa gaggtcgagg acggtctgga
     2281 gggccgcggc cgccaccggg tcgtggaccc cgcgcgcccg ggccccaccg ccggggaaca
     2341 gcagctcgtg cgcaatgggc tcggggccac ccaccatcca gcgggtccgg aggacgaagc
     2401 cggactcgac cgggtggagg accgagacgt cgaagcggtc gcccaaggcc tcgtcgagcg
     2461 cctggacgtg cttctcggtg cctccggcgg ccggtgacgg ctggtgcagc acctgcagca
     2521 cgtgcggccg cgccgggttc cgctcggtga gcgccagctc gagggccttg aaggcgacgg
     2581 ccaggggatg ctcggcgcgc tcgcgggcgt tggtcgcccg gaagaacggg tagcgctccc
     2641 gcagcacggc cgagccccgg gccaggcccg ccacccgctc ggcgccgaac gacaccccac
     2701 cccagtggtg gacgtaggtg gcgtcctcga cgaggtggcg gaagcccagc cgcgtggccc
     2761 ggaggcagaa gtcgacctct tcgccgtagc cccgcccgaa gtcgcgctcg tcgagggggc
     2821 cacaccggtc gatcgcctcc cgggtgacgt agaggcagaa gcccacgccg gtgatcacct
     2881 cgggccgcag gccgagcgac tgctcccgga cgaagtccgc gcaggcgtcg atccggggct
     2941 ccggcccgtc caggccgaac tcggccacga ccgggggcgg cagcgtgcag aacgagccgg
     3001 agctggtcag cggggtcacg gtggcgacgt cctcgccccg ggcggcgtcg gccaaggcgt
     3061 ccagccaccc ggccgtcacg acggtgtcgg cgttgaggac gaccacgtcg ccgtcggcgt
     3121 ggcggatgcc ccggttgacc gaggccacga agccgacgtt gtccgggttg cgcaggaggg
     3181 tcacggcgac cggcgccggc tcggcggcga cccgctccag ctcggcgcgc accgcggggt
     3241 ccgggctggc gtcgtcgacg accaccaggt cgaacgggac ggtgagggcc ggcgcgtggt
     3301 cgagcacgct ggcgaggcac cgcccggtct cggccgcccc gccgtagacg gggacgacga
     3361 cgctgacccg tggcgtgacc gtcaccccgg gacgtcctgg cggggtggca ccgggtgggg
     3421 accggagcag agccggttga cgagctcggc gaacgtcgcc gcgcccaccg aggtcccgat
     3481 gcgggcccgg agacgacccg atgcccgccc gatgcgccag tccaccagcg ccggctcgcg
     3541 gccgaggacc gggtcgacct cgacgacgac ggtccggcgg tcggacccgc accacgggga
     3601 cgcgaccgcg gcggcgccgg cgtccagggc caccacgacc tggggccgga gcgcggcgat
     3661 cgaggccggg tccgggcggc acggccgggt cagttggggc ccctcggcga cctctcccgc
     3721 cgccaggcgg gcctccacgt cctcggtgag gaggtcgttc accaggcgaa gggcccaccg
     3781 gtcctcccca ccacccgcct cgacggcgag gaacaccacc actggccgga ccggaaaggg
     3841 cgaacggccc tcgccgtgag ccacctcgat cggcccgggg tcggcaaggg ggtcgtggac
     3901 gtgcgctccc ggatcgaggg tcacgggccc ggcgacatgc acctgaccgc cgtcgacctc
     3961 gtgctcgacg tcctcgcgca cgagcacggc gcccgcgacc ggaccggcga ccggttccag
     4021 gtgcgccggc cggagcgcac gccactcggc gtgggtgcgg tagctgccgc ccggccgcag
     4081 tggccccgag gcggcgccca ccccggccac ggtggacggc gccgccgcca aagcccgctc
     4141 gagccggtcg atcgagatcc agtcgaggca ccaaccgtcc ggcagcacca gggtgggacc
     4201 ggcggccggg tccgcgggcg ccatcacccg gtggtgccct ccatccggcg ccggaccgag
     4261 cgcacccgcc gccggacccg cgtccccagc tgtgcgtccc gctgccggtt ccgggcccgg
     4321 atctcctcgg tcagcgccac gacctgagcc cgcgcctccg ccagctggcg ggtgtccgcc
     4381 ggcgcccgta gccagccccg ccggatgc
//
LOCUS       DASTFV010000309         3669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1153848, whole genome shotgun sequence.
ACCESSION   DASTFV010000309 DASTFV010000000
VERSION     DASTFV010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153848"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFP02_05820"
     CDS             <1..194
                     /locus_tag="VFP02_05820"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET9542575.1"
                     /translation="LLAFLLHHAQDPNFQVRWRWRADDVAVWDERCTNHRALSDHFPR
                     HRVMRRVTVDGDRPVYTPT"
     gene            complement(256..1314)
                     /locus_tag="VFP02_05825"
     CDS             complement(256..1314)
                     /locus_tag="VFP02_05825"
                     /inference="COORDINATES: protein motif:HMM:NF040257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542576.1"
                     /translation="MTYRVIQWATGGVGRAAIEGVLDHPELELAGCWVHSADKDGRDV
                     GELIGRAPLGVAATTDIDALLAVDADAVLYSPVMADPAVVDRILRSGKNVVTPLGWFY
                     PKGQDTSRLEEACAEGGVTLHGTGIHPGGITERFPLMVSALSNAITHVRAEEFSDIRT
                     YAAPDVVRHVMLFGATPEAAAESIMLNVLGAGFIQSVHMVADVLGFALDPELTTSHEV
                     AVATAPIDSPIGPIEPGLVAAQRFRWVATVGGEPVVTAAVNWFMGQEDLDPGWTFGPE
                     GERFEVEITGDPPVQLSFHGLHPDSIAEGLERNRGIVATAMHCVNAVPYVCAAAPGLQ
                     TYLDLPLVAGRAAPGLRG"
     gene            complement(1345..1743)
                     /locus_tag="VFP02_05830"
     CDS             complement(1345..1743)
                     /locus_tag="VFP02_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HET9542577.1"
                     /translation="MADGDTLFDRAGGQPWFDALVERFYEAVEDDPLLRPIYPEDLGP
                     GKRGLALFLGQYWGGPDAYSAERGHPRLRARHLPFAIDTPERDAWVVLMTAAVRAGGL
                     SAEDEADFLAYVETAATHMINQHPLGLRPS"
     gene            complement(1778..2221)
                     /locus_tag="VFP02_05835"
     CDS             complement(1778..2221)
                     /locus_tag="VFP02_05835"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9542578.1"
                     /translation="MAEFVRDVVIAASPETIFGFLTEADKHRQWEGTEVELDPRPGGV
                     YSVLVAGEYHGVGEFVEVVPYEKIVHTFGWNMPDNAMLPGSTMVEITLEPEGDKTRLR
                     LRHYDLPDDQVATHAEGWTHYLDRLALVAGGGDAGPDIGPDSQPA"
     gene            complement(2221..2535)
                     /locus_tag="VFP02_05840"
     CDS             complement(2221..2535)
                     /locus_tag="VFP02_05840"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9542579.1"
                     /translation="MDAALKALAEPRRRDILRLVWSQELAAGEIAARFDVTRPAISQH
                     LRVLSEADLVTERRDGTRRLYRARPDTVEELQAFLARFWDERLDRLRDVAEAAERDKE
                     RP"
     gene            complement(2589..3623)
                     /locus_tag="VFP02_05845"
     CDS             complement(2589..3623)
                     /locus_tag="VFP02_05845"
                     /inference="COORDINATES: protein motif:HMM:NF040257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542580.1"
                     /translation="MSHKVIQWATGTVGIHAVPAIVHHPALELVGLWVHSDDKAGRDA
                     GELCGIDPVGVTATQDVDALLALDADCICYTAHSDVRPGEVVDDICRMLAAGKNVVNT
                     SFVALLYPEQAGFGDQLEAACQEGGTTFFTSGIDPGYGNAGITIGALGLCKEVRTVRM
                     MEIVNYATWDNPFTMFEIMGFGKADEAQSLLLAPGSTTLAWGPVVALVAASLGLELDE
                     IVEDHEVLLADVDVTIASGVVEAGTISGMRFEIKGMVDGEERVVVEHVTRLRDDDAPH
                     WPQGEGYRILVEGEPNVKLEVTLSSDLGDHNHAGCLATAMHVINAIPAVVAADPGVLT
                     LLDLTPRFRF"
BASE COUNT          510 a         1261 c         1357 g          541 t
ORIGIN      
        1 ccctcctggc gttcctgctc catcacgccc aggaccccaa cttccaggtc cgctggcgtt
       61 ggcgggccga cgacgtggcg gtgtgggacg agcgctgcac caaccaccgc gccctcagcg
      121 accacttccc ccgccaccgc gtcatgcgcc gggtcaccgt cgacggcgac cgccccgtct
      181 acacccccac ctgaacttgc gctcgctgca ccacggatcc gtggtgtgat cggcgcacgt
      241 tcgagggggc tcggctcagc cgcgtaggcc cggcgcggcg cggccggcga cgagcgggag
      301 gtcgaggtag gtctggaggc cgggcgcggc ggcacagacg tagggcacgg cgttcacgca
      361 gtgcatggcc gtggccacga tcccccggtt gcgctccagg ccctcggcga tggagtccgg
      421 gtggaggccg tggaagctga gctggaccgg cgggtcgccg gtgatctcga cctcgaagcg
      481 ctcgccctcg gggccgaacg tccaccccgg atcgaggtcc tcctggccca tgaaccagtt
      541 caccgccgcg gtcacgacgg gctcgccgcc gaccgtggcc acccagcgga accgctgcgc
      601 ggccaccagg cccggctcga tggggccgat gggggagtcg atgggcgccg tggccacggc
      661 cacctcgtgg ctggtcgtca gctcggggtc gagggcgaag cccaggacgt cggccaccat
      721 gtggaccgac tggatgaacc cggcgccgag gacgttcagc atgatgctct cggccgccgc
      781 ctcgggcgtg gccccgaaga gcatcacgtg gcgcaccacg tcgggggcgg cgtaggtgcg
      841 gatgtcgctg aactcctcgg cccggacgtg ggtgatggcg ttggacaggg ccgacaccat
      901 cagggggaag cgctcggtga tcccaccggg gtggatgccc gtgccgtgca gggtcacgcc
      961 gccctcggcg caggcctcct ccagccggct cgtgtcctgg cccttcgggt agaaccagcc
     1021 gaggggcgtg accacgttct tgcccgagcg caggatgcgg tcgacgaccg cggggtcggc
     1081 catgaccggc gagtacagga cggcgtcggc gtccacggcc agcagggcgt cgatgtcggt
     1141 ggtggcggcc acgccgaggg gggcccggcc gatgagctcg cccacgtcac ggccgtcctt
     1201 gtcggcgctg tggacccagc agccggccag ctccagctcg gggtggtcga gcacgccttc
     1261 gatggcggcc cggcccacgc cgccggtcgc ccactggatc acgcggtagg tcatggcctc
     1321 atcatggccc ttgccgcggc cgggtcagga cggacgcagg cccagggggt gctggttgat
     1381 catgtgggtg gcggcggtct ccacgtaggc gaggaagtca gcctcgtcct cggccgacag
     1441 gcctccggcg cgcacggcgg cggtcatgag gacgacccag gcgtcgcgct cgggggtgtc
     1501 gatggcgaag ggcaggtggc gggcccgcag ccggggatgg ccccgctcgg ccgagtaggc
     1561 gtcggggccg ccccagtact ggcccaggaa gagggccagg ccccgcttcc ccggcccgag
     1621 gtcctcggga tagatgggcc ggagcagcgg gtcgtcctcg acggcctcat agaagcgctc
     1681 gacgagggcg tcgaaccagg gctggccgcc cgcccggtcg aagagggtgt caccgtcggc
     1741 caccgtcgca gtatggcggt ggtcgacgag cgcgcggtca ggcgggctgg ctgtccggac
     1801 cgatgtcggg gccggcgtcg cccccgccgg cgacgagggc caggcggtcc aggtagtggg
     1861 tccacccctc ggcgtgcgtg gccacctggt cgtcagggag gtcgtagtga cggagacgca
     1921 accgggtctt gtcgccctcg ggctcgaggg tgatctcgac catcgtcgag ccggggagca
     1981 tggcgttgtc gggcatgttc cagccgaagg tgtgcacgat cttctcgtag gggacgactt
     2041 cgacgaactc gccgacaccg tggtactccc cggcgacgag gaccgagtac acgccgcccg
     2101 gccgcgggtc gagctcgacc tcggtcccct cccactgtcg gtgcttgtcc gcctcggtca
     2161 ggaacccgaa gatcgtctcc gggctggcgg cgatcaccac gtcgcgtacg aattcggcca
     2221 tcagggtcgc tccttgtctc gctcggcggc ctcggcgacg tcacggagac ggtcgaggcg
     2281 ctcatcccag aaccgggcga ggaacgcttg gagctcctcg accgtgtccg gccgcgcccg
     2341 gtagaggcgg cgcgtgccgt cgcggcgctc ggtcaccaga tcggcctcgg acagcacccg
     2401 gaggtgttgg gagatggccg gtcgggtgac gtcgaagcgg gccgcgatct cgcccgccgc
     2461 cagctcctgg ctccacacca gccggaggat gtcccgacgg cggggctcgg ccagggcctt
     2521 gagggcggcg tccaccccgc tattgttagc tcttacttac gtaagcgtca agtttcagtg
     2581 gtcagacgtc agaaccggaa gcggggggtg aggtcgagga gcgtgaggac gccggggtcg
     2641 gcggcgacca cggcggggat ggcgttgatc acatgcatgg cggtggccag gcagccggcg
     2701 tggttgtggt ccccgaggtc ggacgacagc gtgacctcca gcttcacgtt gggctccccc
     2761 tcgaccagga tccggtagcc ctcgccctgg ggccagtgcg gggcgtcgtc gtcccggaga
     2821 cgggtgacgt gctcgaccac cacccgctcc tcgccgtcga ccatcccctt gatctcgaag
     2881 cgcatccccg agatggtccc ggcctcgacc acgcccgagg cgatggtgac gtccacgtcg
     2941 gccaggagca cctcgtggtc ctcgacgatc tcgtccagct cgaggcccag cgacgccgcg
     3001 accagggcga ccaccggccc ccaggccagg gtcgtgctcc cgggcgccag cagcagggac
     3061 tgggcctcgt cggccttccc gaaccccatg atctcgaaca tggtgaaggg gttgtcccag
     3121 gtggcgtagt tgacgatctc catcatccgg acggtgcgga cctccttgca caacccgagg
     3181 gccccgatgg tgatgccggc gttgccgtag cccgggtcga tgcccgaggt gaagaaggtc
     3241 gtcccgccct cctggcaggc ggcctccagc tggtcgccga acccggcctg ctcggggtac
     3301 agcagggcca cgaacgacgt gttgaccacg ttcttgcccg ccgccagcat ccggcagatg
     3361 tcgtcgacca cttcgccggg gcggacgtcg gagtgggccg tgtaacagat gcagtcggcg
     3421 tcgagggcga gcagggcgtc gacgtcctgg gtggcggtca cgcccaccgg gtcgatgccg
     3481 cacagctccc cggcgtcgcg gccggccttg tcgtcgctgt ggacccagag gccgaccagc
     3541 tcgagagcgg ggtggtggac gatggcgggt acggcgtgga tgccgacggt gcccgtcgcc
     3601 cattggatga ccttgtggct cattccggcg accgtactgc ggcggggact acggtcgatt
     3661 ccggggacc
//
LOCUS       DASTFV010000310         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1160452, whole genome shotgun sequence.
ACCESSION   DASTFV010000310 DASTFV010000000
VERSION     DASTFV010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160452"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="VFP02_05850"
     CDS             complement(<1..1152)
                     /locus_tag="VFP02_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9542581.1"
                     /translation="MAVTEDVRTEVRAWLDENWDPDLTVADWWQRLADSGWAMPSWPE
                     EHSGRGLARPEANAVGVEIGEAGALGPPGGLGTMLAAPTIIAHGSDEQKQRFLPKIAN
                     GQEAWCQLFSEPGAGSDLAGLQCKAVKDGDEWIVNGQKVWTSGGQHADLGMLIARTDP
                     DAPKHKGISYFGIPMHQPGIDVRPLVEMTGRALFNEVFLDDARVADDLLIGGLGNGWA
                     VANTTLMNERASLGGGGGSSATSLALPGSVAGHLERRAGDFAAKGGRGGTATILAGRS
                     TKLLQNLVAQLGKNDDPLIRQDLMRLYSMDQISGWGVQRAKAGQASPALPNLAKLMMS
                     HMTRLSRDLGLRIVGPSGTVMGPDTPGGGMIQELALFSPAPSIYGGTDEV"
     gene            1180..>2355
                     /locus_tag="VFP02_05855"
     CDS             1180..>2355
                     /locus_tag="VFP02_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9542582.1"
                     /translation="MAVTDLASVPLDEIDLTDPDAYVARVPHEQFATLRREAPVFWHE
                     DAEGGFWCVTRHADIVAVNRDWETYSSYKGAVYLWTPDELEMQRLMMLNMDPPEHAKL
                     RKLVNRGFTPRRIRELQDVLARRATTIVDEVIEAGSCDMVEAVAAELPLQAIAEFLGV
                     PQEDRHLVFKWSNTMIGSDDPEYASEKDEAGQTAETMAASAELYAYAQGLAEDRRANP
                     RDDIVSDLVQAEIDGDVLSDTDFNLFFLLLAVAGNETTRNAISHSVLALAENPDQRAW
                     LREDPTRWDSALEEMLRWSTPVMHFRRTATRDAELGGQAIAEGDRVVIWHISGNRDED
                     VFTDPFTFDLGRTPNEHLGAASHVAFGGGGPHFCLGANLAKAEMRVMLEEVVNRLGDF
                     "
BASE COUNT          337 a          827 c          830 g          361 t
ORIGIN      
        1 cacctcgtcg gtgccgccgt agatcgacgg ggccgggctg aacagggcca actcctggat
       61 catcccgccg cccggcgtgt ccgggcccat cacggttccc gacggtccga cgattcgcag
      121 gccgaggtcg cgggagagcc gggtcatgtg gctcatcatc agcttggcca ggttgggcag
      181 agccggtgag gcctggcccg ctttggcccg ctgcacgccc cagccgctga tctggtccat
      241 ggagtagaga cgcatcaggt cctggcggat cagcggatcg tcgttcttgc ccagctgggc
      301 cacgaggttc tgcagcagct tggtgctgcg cccggccagg atcgtggcgg tgccaccgcg
      361 gccacccttg gccgcgaagt cacccgcccg acgctcgagg tgcccggcga cgctgcccgg
      421 cagtgccagc gaggtggcgg acgagccgcc gcccccgccg aggctggccc gctcgttcat
      481 gagcgtggtg ttggccaccg cccagccgtt gccgagaccg ccgatgagga ggtcgtcggc
      541 cacccgggcg tcgtcgagga agacctcgtt gaacagcgca cggcctgtca tctcgaccag
      601 gggccggacg tcgatgccgg gctggtgcat cgggatgccg aagtagctga tgcccttgtg
      661 cttgggggcg tccgggtcgg tgcgggcgat gagcatgccc aggtcggcgt gctggccgcc
      721 actggtccac accttctggc cgttgacgat ccactcgtcg ccgtccttga cggccttgca
      781 ctggaggccg gcgaggtcgg agccggcgcc gggctcgctg aagagctggc accaggcttc
      841 ctggccgttc gcgatcttgg gcaggaatcg ctgcttctgc tcgtcgctgc cgtgggcgat
      901 gatcgtcggg gcggccagca tcgtgcccag ccctcctggc ggtccgaggg cgccggcctc
      961 gccgatctcg accccgacgg cgttggcctc gggacgggcc agcccccggc ccgagtgctc
     1021 ctcgggccag ctcggcatgg cccagccgga gtcggcgagg cgttgccacc agtcggccac
     1081 ggtcaggtcg gggtcccagt tctcgtccag ccaggcgcgg acctcggtcc gcacatcctc
     1141 agtcacagcc atccgccgag cttacgtagg gtggtcgccg tggccgtcac cgacctggca
     1201 tccgttcccc tcgacgagat cgacctgacc gatcccgacg cctacgtggc gcgggtcccg
     1261 cacgagcagt tcgccaccct gcgccgggag gcgccggtgt tctggcacga ggacgccgaa
     1321 ggggggttct ggtgcgtgac ccgccacgcc gacatcgtcg cggtgaaccg ggactgggag
     1381 acgtactcct cctacaaggg ggcggtgtac ctctggacgc cggacgagct cgagatgcag
     1441 cggctgatga tgctgaacat ggacccgccc gagcacgcca agctgcggaa gctggtcaac
     1501 cgggggttca cgccgaggcg catccgggag ctccaggatg tcctggcccg gcgggcgacg
     1561 acgatcgtcg acgaggtcat cgaggcgggg tcctgcgaca tggtggaggc cgtcgccgcc
     1621 gagctcccgc tccaggccat cgccgagttc ctgggcgtgc cccaggagga ccgtcacctg
     1681 gtcttcaagt ggagcaacac catgatcggc tccgacgacc cggagtacgc ctccgagaag
     1741 gacgaggcgg gccagaccgc cgagacgatg gcggcctcgg ccgagctcta cgcctacgcc
     1801 cagggcctgg ccgaggaccg gcgggccaac ccccgtgacg acatcgtgtc cgacctggtc
     1861 caggccgaga tcgacggcga cgtgctgagc gacaccgact tcaacctctt cttcctcctc
     1921 ctcgccgtgg ccgggaacga gaccacccgc aacgccatct cccactcggt gctggccctg
     1981 gccgagaacc ccgaccagcg ggcgtggctg cgcgaggacc cgacccgctg ggactccgcc
     2041 ctggaggaga tgctgcgctg gtccaccccg gtcatgcact tccgccggac ggccactcgg
     2101 gacgccgagc tgggcgggca ggccatcgcc gagggtgacc gggtggtgat ctggcacatc
     2161 tcgggcaacc gggatgagga cgtgttcacc gatcccttca cgttcgacct cgggcgcacg
     2221 cccaatgagc acctcggggc ggccagccac gtcgccttcg gcggcggcgg tccgcacttc
     2281 tgcctcggcg ccaacctggc caaggccgag atgcgggtga tgttggagga ggtggtcaat
     2341 cgcctcgggg acttc
//
LOCUS       DASTFV010000311         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1171472, whole genome shotgun sequence.
ACCESSION   DASTFV010000311 DASTFV010000000
VERSION     DASTFV010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171472"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /gene="menD"
                     /locus_tag="VFP02_05860"
     CDS             complement(<1..781)
                     /gene="menD"
                     /locus_tag="VFP02_05860"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HET9542583.1"
                     /translation="MDESTVAATFCATLVDEFARGGVTDAVVAPGSRSTPLTLALARD
                     ERIRVHVHVDERSAGFLALGLGLGTGRPAVVVTTSGTAAVELHPAVAEADLAGVPLLA
                     CTTDRPPELHGVGAPQTVDQAGLYGRSARAMVEPGVPREAAAWSWRSLAARAVLDATG
                     SRPGPVHLNLAFTEPLLGEPAALPAGRADGAPWHQAVPMPTTVTVVEELAGRRGVIVA
                     GRGSEPTVVTRVAEVLGWPVLADPLSGCRRLGPRTVAGFDLL"
     gene            807..1535
                     /locus_tag="VFP02_05865"
     CDS             807..1535
                     /locus_tag="VFP02_05865"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9542584.1"
                     /translation="MSSPPLVLAHGFTQNARCWGPFFTALGDQRPVEAVDLAGHGSTP
                     VARDLTEAGHRLGRAGGWGCYLGYSLGGRVALHLALTQPDLVTALVLIGAHPGIENGD
                     EREARRRADDELADHLEAVGLDAFLEEWLAQPLFASLPPEAVQRTERLTNDPAALATT
                     LRHLSTGRQVPLWDHLPKLSMPVLILAGEHDEAYRRLSRQAAATIGQNASYESVPDAG
                     HAAHLEQPAHVAQRVRAFLTASGL"
     gene            complement(1537..>2016)
                     /gene="menA"
                     /locus_tag="VFP02_05870"
     CDS             complement(1537..>2016)
                     /gene="menA"
                     /locus_tag="VFP02_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HET9542585.1"
                     /translation="PKPYGYLGLGEVFVFVFFGLVATVGSFYVQTSEVTALAVCAAVP
                     VGLLATALLVVNNLRDIPTDAEAGKRTLAVRLGDRTTRIVYVTMLVDVFLLVVVLGVT
                     ARMPALLGLVGIVFAIPPIRGVQAGATGPELIPVLGATGKAQLVTGLLLAFGLALGG"
BASE COUNT          299 a          778 c          703 g          236 t
ORIGIN      
        1 ccaggaggtc gaagccggca acggtcctcg gaccaaggcg gcgacagccg ctcagcggat
       61 cagcgagtac gggccacccg agtacctccg cgacccgggt gacgaccgtg ggctcgctgc
      121 cccggcccgc cacgatcacg ccgcgtcgac cggccagctc ctccaccaca gtgaccgtcg
      181 tcggcatcgg gacggcctgg tgccatgggg cgccgtcggc tcggcctgcc ggcaacgcgg
      241 ccggctcgcc cagcagcggt tccgtgaacg cgaggttcag gtggacgggg cccggccggg
      301 atcccgtcgc atccaggacc gcccgggcgg ccagcgagcg ccatgaccac gcggccgcct
      361 cccgcggcac gcccggctcg accatggccc gcgcggaccg gccgtagagg ccggcctggt
      421 cgacggtctg cggggccccc acgccatgga gctccggcgg tcggtccgtt gtgcaggcca
      481 gcaggggcac acctgccagg tcggcctcgg ccacggcggg gtgcagctcg accgccgccg
      541 tgccactggt cgtcaccacg accgcgggac ggccggtccc cagcccgagg ccgagggcca
      601 ggaatcccgc ggaccgctcg tcgacgtgca cgtgcacccg gatgcgctcg tcccgggcca
      661 gcgccagggt caacggtgtc gaccgggacc cgggagcgac gacggcatcg gtgacgcccc
      721 cccgggcgaa ctcgtcgacc aaggtggcgc agaacgtcgc agccaccgtc gactcgtcca
      781 cggcccagga ctctagggtc gcgccggtga gcagcccgcc cctggtcctg gcccacgggt
      841 tcacccagaa cgcccggtgc tgggggccgt tcttcaccgc cctcggcgat cagcgtccgg
      901 tggaggccgt cgacctcgcc ggccacgggt ccacgccggt tgctcgggac ctgaccgagg
      961 ccggccaccg actgggtcgg gccggcgggt ggggttgcta tctcggctac tccctcggcg
     1021 gtcgggtggc cctgcacctg gccctcaccc agcccgacct ggtcaccgcc ctcgtcctca
     1081 tcggcgccca ccccggcatc gagaacggcg acgaacggga ggctcgccga cgagccgacg
     1141 acgagctggc cgaccacctc gaggcggtcg ggctcgacgc gttcctcgag gagtggctgg
     1201 cccagccgct gttcgcctcg ctgccgccgg aggcggtcca acgcaccgaa cggctgacca
     1261 atgaccccgc ggctctggcg accaccctgc gccacctcag caccgggcgt caggtcccgc
     1321 tctgggacca cctcccgaag ctctcgatgc cggtgctcat cctcgccggc gagcacgacg
     1381 aggcctatcg gcgcctcagt cgccaggccg cagccaccat cgggcagaac gccagttacg
     1441 agagcgtgcc cgacgccggg catgccgccc acctcgaaca gccggcccat gtggcccaac
     1501 gggtccgggc gttcctcacg gcctctggcc tctgagtcag ccgccgaggg ccaggccgaa
     1561 cgccaacagg aggcccgtga ccagctgcgc cttgcccgtg gcaccgagga cggggatgag
     1621 ctcgggcccc gtggccccgg cctgcacccc tcggatgggg gggatggcga acacgatccc
     1681 gacgaggccc agcagggccg gcatccgggc cgtgacgccg agcacgacca ccagcaggaa
     1741 gacgtcgacg agcatggtca cgtacacgat ccgcgtcgtc cggtcgccga ggcgcacggc
     1801 cagcgtgcgc ttgcctgctt cggcgtcggt cgggatgtcc cgcaggttgt tgaccaccag
     1861 caacgcggtg gccaacaggc ccaccgggac cgcggcacag acggccagcg cggtcacctc
     1921 gctggtctgc acgtagaagg acccgaccgt ggccaccagg ccgaagaaga cgaagacgaa
     1981 gacctccccc aggccgaggt acccgtaggg cttggg
//
LOCUS       DASTFV010000312         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1171597, whole genome shotgun sequence.
ACCESSION   DASTFV010000312 DASTFV010000000
VERSION     DASTFV010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171597"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..634
                     /locus_tag="VFP02_05875"
     CDS             53..634
                     /locus_tag="VFP02_05875"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9542586.1"
                     /translation="MTGRTDDLSALVVAAQRGDERAFESLVRATHAETYTLAFRLTGD
                     DEDARDVTQDTYLRAYRALDRFRGDARFQTWLYRITANSASTLLGQRARHRHDELTEE
                     APLADDRITIDPVARAEGDSLRHHLTDALRRLPPGLRSVVVLRDVYDLPHDAIAEELD
                     ISVTAAKVRLHRARRRLREDLYAQSHEGEAHAV"
     gene            624..989
                     /locus_tag="VFP02_05880"
     CDS             624..989
                     /locus_tag="VFP02_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542587.1"
                     /translation="MRCDQVADRLPAAAEGLAALDPAARRHVERCLRCQADAVQYRRV
                     LRAMRALRTEVLVPTPGLLPEILAAIGEAGEQQALRNMVRGRRAAYLGGLAAATAAGA
                     AGAVVLTLRSRRSKVRLAG"
     gene            1020..>2244
                     /gene="bioA"
                     /locus_tag="VFP02_05885"
     CDS             1020..>2244
                     /gene="bioA"
                     /locus_tag="VFP02_05885"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HET9542588.1"
                     /translation="MTGEAEDWVARDAAAVWHGFTQMSTYGANAPIIVAEAEGRELID
                     VDGRRYLDAISSLWVTTLGHRVPELDQALRDQIDRVAHSTMLGNGNQVVIALAEALRE
                     VVPVDDAHVLFAGDGAAAVEQALKIAFQFWTNQGVTGRTRYLAFGDAYHGDTIGAISL
                     GAGGFGTDVFDPLRFPVLRAPSFADPDCFAVAARVVAAHAPELAAVVIEPLVQGAAGM
                     QLADPEDVAALAAACREHDVLLIADEVAVGFGRTGTLFASEQCGIRPDLLCLGKGLTG
                     GYLPMSATVASDRVFAAFLGPDLSERTLYHGHSYSGNALGAAVALRHLQLVTEGDVLA
                     DVRTAAAHLAGRLAEAIAPLDAVGAIRQHGLMVGVELAPSAEGLRWGRRVCAGAVERG
                     VLLRPLGDVVVLMPPL"
BASE COUNT          269 a          859 c          803 g          313 t
ORIGIN      
        1 agggggaacc gcggcgcgta acgtcctcgg caatggctgg gtctgattgc tcgtgacggg
       61 ccgtaccgac gacctcagcg ctctcgttgt cgccgcccag cggggcgacg agcgtgcctt
      121 cgagtcgttg gtgcgtgcca cccacgcgga gacctacacg ctggccttcc ggctcaccgg
      181 tgacgacgag gacgctcgcg acgtcaccca ggacacctac ctccgggcct accgggcgct
      241 cgaccgcttc cgcggcgacg cccggttcca gacctggctg taccggatca cggccaacag
      301 cgccagcacc ctcctcggcc aacgggcccg gcaccgtcac gacgagctga ccgaggaggc
      361 tcctttggcc gacgaccgca tcaccatcga ccccgtggcc cgggccgagg gcgacagcct
      421 gcgccaccac ctgaccgacg cgctgcgccg gctcccgccg ggcctgcggt ccgtcgtggt
      481 cctgcgcgac gtctacgacc tgccccacga cgccatcgcc gaggagctgg acatctcggt
      541 cacggcggcc aaggtgcgcc tccaccgggc ccgccgccgc ctgcgtgagg acctctacgc
      601 ccagtcccac gagggagaag cccatgcggt gtgaccaggt cgccgaccgg ctgcccgcgg
      661 cggccgaggg cctcgccgcc ctcgatccgg cggcccgccg ccacgtcgag cgctgcctgc
      721 gctgccaggc cgacgccgtg cagtaccgcc gggtcctgcg ggccatgcgg gccctgcgca
      781 ccgaggtcct cgtgcccacg ccgggcctgc tccccgagat cctggccgcc atcggcgagg
      841 ccggagagca gcaggccctg cgcaacatgg tccggggccg tcgggccgcc tacctcggtg
      901 ggctggcggc ggcgacggcg gccggcgcgg ccggggcggt ggtcctgacc ctgcgctccc
      961 gccgcagcaa ggtgcgcctg gccggctgat cggcggggcg ctcaggtgat actgcggcgg
     1021 tgaccgggga ggcggaggac tgggtggcgc gcgacgccgc cgcggtgtgg cacgggttca
     1081 cccagatgtc gacctacggc gccaacgccc cgatcatcgt ggccgaggcc gagggccggg
     1141 agctgatcga cgtcgacggg cggcgctacc tcgacgccat ctcctctctg tgggtcacca
     1201 ccctcggcca tcgggtcccc gagctggacc aggccctccg ggaccagatc gaccgcgtgg
     1261 cccactccac gatgctgggc aacggcaacc aggtcgtcat cgcgctggcc gaggcgctcc
     1321 gggaggtcgt gcccgtagac gacgcccacg tgctcttcgc cggcgacggt gccgcggccg
     1381 tcgagcaggc tctcaagatc gccttccagt tctggaccaa ccagggcgtc accggccgga
     1441 cccggtacct ggccttcggc gacgcctacc acggggacac gatcggggcg atctcgctcg
     1501 gggcgggcgg gttcggcacc gatgtgttcg acccgctgcg cttcccggtc ctccgggcgc
     1561 cctccttcgc cgatccggac tgcttcgcgg tggcggctcg ggtggtggcc gcccacgccc
     1621 cggagttggc cgccgtcgtc atcgagcccc tcgtgcaggg cgccgcgggc atgcagctgg
     1681 ccgacccgga ggacgtggcc gccctggccg ccgcctgccg cgagcacgac gtgctgctca
     1741 tcgccgacga ggtggccgtc ggcttcggcc gcaccgggac cctgttcgcc agcgaacagt
     1801 gcggcatccg gcccgatctg ctctgcctgg gcaagggcct gaccggcggc tatctgccga
     1861 tgtcggccac cgtggcctcg gaccgggtgt tcgccgcctt cctgggaccg gacctctccg
     1921 agcggacgct gtaccacggg cactcctaca gcgggaacgc cctgggcgcg gcggtggccc
     1981 tgcgtcacct ccagctcgtc accgagggtg acgtcttggc cgacgtccgg accgcggccg
     2041 cccacctggc cggccgtttg gccgaggcga tcgcccccct cgacgccgtg ggggcgatcc
     2101 gccagcacgg cctcatggtc ggcgtggagc tcgccccgtc ggcggagggc ctgcgttggg
     2161 gtcgacgggt ctgcgccgga gcggtcgagc ggggtgtcct cctgcgcccg ctgggtgacg
     2221 tcgtcgtgct gatgccgccg ctga
//
LOCUS       DASTFV010000313         6126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1176427, whole genome shotgun sequence.
ACCESSION   DASTFV010000313 DASTFV010000000
VERSION     DASTFV010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176427"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VFP02_05890"
     CDS             complement(<1..276)
                     /locus_tag="VFP02_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542589.1"
                     /translation="MAPAAPVVRAAAVLTAVALVLVVGRGVLLDKHAHRLEHLLEQAE
                     AEGPRDLTPYDGLGTWVDAYDYGPAYQTDGHEPAVTPDDVAAMDAAGV"
     gene            complement(327..923)
                     /locus_tag="VFP02_05895"
     CDS             complement(327..923)
                     /locus_tag="VFP02_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542590.1"
                     /translation="MSTRSRAIALGVALLLILAACGSDDDEAQPATVSSTTASTTTTV
                     GTVPFESTRYPYRLVMPADWQLSEVDGAWTSLDQFAAGEEVPGEEVLSTPDRATFLVV
                     NSMALPEGTTPSAWTTAFGALVADGLVDGCTRTSGEGSVAGETAQVVEDTCDDDVILG
                     RSLVHGSRGWYFTTRGTDDAATTALLDDLVASIEFTDD"
     gene            complement(1079..4189)
                     /locus_tag="VFP02_05900"
     CDS             complement(1079..4189)
                     /locus_tag="VFP02_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542591.1"
                     /translation="MSGPATNLTAFVARLPGIVPRVISGAVARTDKAWDGIGARLPTR
                     GLPGEALLVAVVVGYAMVNVARSTLDTGIQKIHAVGVSGVSANSLHDGRDAIDTAFMT
                     WNDGDERLSALVRFHPVTLARAQTVLELLLVPLTLVLFYGLLKWLRGRVGTGPRSPAR
                     AAVLTLLEVAAIGVLGYVVLGLFNQLAELAILANGDSGGPFPAVLALTGPVRGLLLPL
                     FLVPVAVALLQMHRDRTSGTGPPPEPVWRGAPTAYRVLFVVVILHAVLVLASIPGEQT
                     ADALRLWLTNPLLGVTGLVFTALFCVALAAIALRIGTDANPKRVVLARAGTARLIGIG
                     FALAAVGGVARYGFDQAWGAGVLAAGILVLLAGLLSFPLAPAAAGDPPTTATTAASLE
                     VVEPVTSVKRLVPALLVVAPFTVLAVSLVRAAIPTILDESSSGWLAGIAVITGACAVI
                     GWCFARLAARWAFKADTATRRRVFRATAWALTAIIAAAYVAVVFDDAQRWSRDLGVVA
                     IIAVFLTAVVLVFGTLGYVMESRALPAVLDFVNFRRLPVILSLVALALLSNQFALPGY
                     HDFQRLDDVALPAESQITAEEAFEQWTAANLTDAQETDEPGDEQAATPMVFVAAAGGG
                     IKAAAFTASTIDCLFQDHDNPNEGHPCNDTDAYNRLFAASGASGGSVGIASVVAERAD
                     GQVGEDWVQDRLGNDLLSPELAWQVMVEVPNAIARFQPGQDRGEVLTDSWTAEFADDE
                     HDPGAEPFYVDQTDDDWSGPQVFFSGTNLNDGCRVNISGVRSAQADGSPEDQQLLAAE
                     ATRSGACNAQRADDGRAPDQAGTRDLVDYLCGQNVDLATAAFLSARFPFVSPTGTTRC
                     PDDDAGTSDSLSIGDGGYRDNSGAGSLMDSWAALDPLVAEFNASHDQCVVPIFVEINT
                     GYSGFGGPSAAGDVAQIIAPPLGAARVFSDLSYGPIEQAAAEFSRPLAPDRQVLVNDE
                     ELPTRFFRVTLVDHPGVTAALGWSLSERAVDDLVGQLDLTENRQALDGLRDLLDPSRA
                     DTLRCA"
     gene            complement(4285..5037)
                     /locus_tag="VFP02_05905"
     CDS             complement(4285..5037)
                     /locus_tag="VFP02_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9542592.1"
                     /translation="MTIRLGIDIGGSGVKGAPVDLDTAQFTEERLRFETPHPATPEAV
                     ATTVTAVAANFAADGTIACTFPGIVQQGVIRSAANMDAAWIGTDAAALFAAATGRPVV
                     VLNDADAAGLAEARFGAARGRQGVVLVLTFGTGIGSALLNDGELVPNTELGHLELHGH
                     DAEHRAAASVRERDDLTWEEWGARVNRYLQHLELLFTPDRFVIGGGVSKRFDKFVELV
                     DVRAEVVPAQLRNQAGIVGAALVAAGREAPPS"
     gene            complement(5034..5960)
                     /locus_tag="VFP02_05910"
     CDS             complement(5034..5960)
                     /locus_tag="VFP02_05910"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET9542593.1"
                     /translation="MALPPDLKELAARVSNWGRWGEDDQRGTLNLIDQTAVLRGVAAA
                     KQGRPFSLAIPFDEDGPQIGLIPGRDNPQREMIAVNQSYTGDTDDFCTSDDRVSMGIQ
                     AATHWDSLAHVGYEGRLYNGVPFSAVDEDGCHRLGIDTVGPLVSRGVLLDVARALGVE
                     TFDDGHPITADDLDAAVALANVEVQPGDVLMVRTGQMAFLARGDKLRFADPSPGLSTR
                     SVAWLRDHDVGAIATDTLVFECWPCEDPTVLLPVHLLQLRDMGLLQGQLWYLDALAED
                     CAADGQYDGLLTATPLPFTRGAGGPVAPTLVK"
BASE COUNT          879 a         2047 c         2376 g          824 t
ORIGIN      
        1 cacgccggct gcgtccatgg cggccacatc gtcaggggtg accgccggct cgtggccatc
       61 ggtctggtac gccggtccgt agtcgtaggc gtcgacccag gtgcccagcc cgtcgtaggg
      121 ggtcaggtcc cggggaccct cggcctcggc ctgctccagg aggtgctcca accggtgggc
      181 gtgcttgtcg agcagcaccc cgcgcccgac gacgagcacg agggccaccg cggtgagaac
      241 ggccgcggca cggacgacgg gtgccgccgg agccatccgg ggaccgtacc ggcgtgcacg
      301 tcgtcgtcgt gctcaggccg tccggctcag tcgtcggtga actcgatcga ggcgacgagg
      361 tcatcgagga gcgcggtggt cgccgcgtcg tcggtgcctc gggtcgtgaa gtaccagccc
      421 cggctgccgt ggaccaggct ccgcccgagg atgacatcgt cgtcgcaggt gtcctcgacc
      481 acctgagcgg tctcgccggc gacggagccc tcgccggagg tgcgggtgca accgtcaacg
      541 aggccgtcgg cgacgagggc gccgaaggct gtggtccaag ccgacggtgt cgtgccttcg
      601 ggaagagcca tcgagttgac caccaggaac gtcgctcgat cgggggtgct gagcacctcc
      661 tcgcccggga cctcctcgcc ggcggcgaac tggtcgaggc tggtccaggc cccgtcgact
      721 tcgctcagct gccagtccgc cggcatcacg agccggtagg ggtagcgggt cgactcgaag
      781 ggcaccgtgc ccactgtggt ggtcgtcgac gcggtcgtcg atgacaccgt ggcgggctgg
      841 gcctcgtcgt cgtcggagcc gcaggcggcg aggatgagga ggagggccac tcccagggcg
      901 atggctcggg atcgagtcga catgaggtcg ctccttccgg atccggtctg tcccggattg
      961 atcatccgcg ccgctacagg cccgggccag ggtgcctgct cacccgtcag tggggggcca
     1021 acccaccccg ctcgtccagg ttccaagagt ggtcgtcggc cgttcccagc ccccttggct
     1081 aggcgcagcg gagggtgtcg gcgcgggacg ggtcgagcag gtcgcgcagg ccgtcgagag
     1141 cctggcggtt ctcggtcagg tcgagttggc cgacgaggtc gtcgacggct cgctcgctga
     1201 gcgaccagcc gagcgcggcg gtgacccccg ggtggtcgac gagggtgacc cggaagaacc
     1261 gggtcgggag ctcctcgtcg ttgacgagca cctgccggtc gggggccagg ggccggctga
     1321 actcggccgc ggcctgctcg atcggaccgt aggacaggtc ggagaacacc cgggcggccc
     1381 ccaggggcgg ggcgatgatc tgggcgacgt cgcccgccgc ggaaggacct ccgaacccgg
     1441 agtagccggt gttgatctcg acgaagatcg ggacgacgca ctggtcgtgg gaggcgttga
     1501 actcggccac cagcgggtcg agcgcggccc aactgtccat cagcgaaccg gccccggagt
     1561 tgtcccggta gccaccgtca ccgatcgaca ggctgtcact ggtgccggcg tcgtcgtcgg
     1621 ggcagcgcgt ggtgcccgtg ggtgagacga acgggaagcg ggcggagagg aacgcggcgg
     1681 tggccagatc gacgttctgg ccgcagaggt agtcgacgag gtcgcgggtc ccggcctgat
     1741 cgggcgctcg tccgtcatcg gcgcgctggg cgttgcaggc tcccgagcgg gtggcctcgg
     1801 ccgccaggag ctgctggtct tccgggctgc cgtcggcctg ggccgatcgc acgcctgaga
     1861 tgttcacccg gcagccgtcg ttcaggttcg tgccgctgaa gaacacctga ggcccggacc
     1921 agtcgtcatc ggtctggtcg acgtagaagg gctcggcgcc aggatcgtgc tcgtcgtccg
     1981 cgaactcggc ggtccaggag tcggtcagga cctcaccccg gtcctggccg ggctggaagc
     2041 gggcgatggc gttcggcacc tcgaccatga cctgccaggc cagctccggc gacaggaggt
     2101 cgttcccgag tcggtcttgg acccagtcct cgccgacctg gccgtcggcc cgctcggcga
     2161 cgacggaggc gatgccgacg ctgcccccgg aggccccgct ggcggcgaac agccggttgt
     2221 acgcgtcggt gtcgttgcag gggtggccct cgttcgggtt gtcgtggtcc tggaagaggc
     2281 agtcgatggt ggaagcggtg aaggcggcgg ccttgatgcc gccaccggcg gcggcgacga
     2341 agaccatcgg ggtcgccgcc tgctcgtcgc ccggctcgtc ggtctcctgc gcgtcggtca
     2401 ggttggccgc ggtccattgc tcgaaggcct cttcggccgt gatctgggac tcggccggca
     2461 gggcgacgtc gtccaaccgc tggaagtcgt ggtagccggg gagggcgaac tggttgctga
     2521 gcagcgccaa ggcgacgagg gacaggatga cgggcagccg gcggaagttg acgaagtcga
     2581 gcacggcggg cagggcccgg ctctccatca cgtagccgag ggtcccgaag acgaggacga
     2641 ccgcggtgag gaacacggcg atgatcgcca cgacgccgag atcacgggac caccgctgcg
     2701 cgtcgtcgaa gacgacggcc acgtacgcgg cggcgatgat cgcggtgagc gcccaggccg
     2761 tggcccggaa gacccggcgt cgggtggccg tgtccgcctt gaacgcccac cgcgccgcga
     2821 gtcgggcgaa gcaccacccg atgacggcac aggcgccggt gatcaccgcg atcccggcca
     2881 gccagccgct cgagctctcg tccaggatcg tcgggatcgc ggcccgtacg aggctgacgg
     2941 ccaacaccgt gaagggggcg acgaccagga gggccggcac caggcgcttc accgacgtga
     3001 ccggctcgac gacctccagg ctcgctgccg tcgtcgccgt cgtcggcgga tcgccggccg
     3061 cggccggggc cagcgggaag ctgagcaggc cggcgaggag caccaggatg ccggcggcca
     3121 gcacgccggc gccccacgcc tggtcgaagc cgtagcgggc gacgccgccg acggcggcga
     3181 gggcgaagcc gatgccgatc agcctcgcgg tcccggcccg ggccaggacc actcgcttgg
     3241 ggttggcgtc ggtaccgatg cggagggcga tggcggccag cgccacgcag aacagggccg
     3301 tgaagacgag gcccgtgacg cccagcagcg ggttcgtgag ccagaggcgc agggcgtcgg
     3361 cggtctgctc accggggatg ctcgcgagga cgagcacggc atggaggatc acgacgacga
     3421 agagcacccg ataggccgtc ggtgcccccc gccacacggg ctccggggga gggccggtgc
     3481 cgctggtgcg gtcccggtgc atctggagca gggccaccgc gaccggcacg aggaacagcg
     3541 gcagcagcag gcctcggacc gggccggtca gcgcgaggac cgcggggaac gggccgccgc
     3601 tgtcgccgtt ggcgaggatg gcgagctcgg cgagctggtt gaacagcccg aggaccacgt
     3661 agcccagcac gccgatcgcg gccacctcga ggagggtgag gaccgcggcg cgtgccggag
     3721 agcgcgggcc cgtgccgact cggccgcgca gccacttgag caggccgtag aagagcacca
     3781 gggtcagggg cacgagcagc agttcgagca cggtctgcgc cctggccagg gtgaccggat
     3841 ggaagcggac gagcgcgctc agccgctcgt caccgtcgtt ccacgtcatg aaggcggtgt
     3901 cgatggcgtc gcgaccgtcg tggagcgagt tggcgctgac gccgctcacc ccgacggcat
     3961 ggatcttctg gatccccgtg tccagggtgg agcgggcgac gttgaccatc gcgtagccga
     4021 cgacgaccgc gaccaggagg gcctcgccgg gcaggccgcg ggtggggagg cgggccccga
     4081 tgccgtccca ggccttgtcg gtccgagcga ccgcgcccga gatcacgcgt ggcacgatcc
     4141 cgggaagccg ggccacgaac gcggtgaggt tggtggcggg tccgctcata ggacgcttcg
     4201 gtcggtgggt cgcggtccgg cgatcgtaga cggcccgtcg ctgcgggtgc cggtgtcgct
     4261 actccgcgtg gtccccgggc ccggtcagga cgggggcgcc tcccggccgg cggccaccag
     4321 ggcggcgccg acgatgccgg cctggttgcg cagctgggca gggacgacct cagcccgcac
     4381 gtcgaccagc tcgacgaact tgtcgaagcg cttgctgacc ccgccgccga tgacgaagcg
     4441 gtcgggggtg aacaacagct cgaggtgctg caggtagcgg ttgacccgcg ccccccactc
     4501 ctcccaggtg agatcgtccc gctcccgaac ggaggccgcg gcccggtgct cggcgtcgtg
     4561 gccgtgcagc tcgaggtggc ccagctcggt gttgggcacc agctcaccgt cgttgagcag
     4621 cgcggagccg atgccggtgc cgaaggtgag gacgaggacg accccctgac ggccgcgggc
     4681 ggcgccgaag cgggcctcgg ccagcccggc ggcgtcggcg tcgttgagca cgacgaccgg
     4741 ccgcccggtg gcggcggcga agagggcggc agcgtcggtg ccgatccagg ccgcgtccat
     4801 gttggccgcg ctccggatca cgccctgctg gacgatgccc gggaaggtgc aggcgatggt
     4861 gccgtcggcc gcgaagttgg cggcgaccgc ggtgaccgtg gtggccacgg cctcgggggt
     4921 ggcgggatgg ggcgtctcga accgaagccg ctcttccgtg aactgcgcgg tgtcgaggtc
     4981 gaccggagcg cccttgaccc cgctgccgcc gatgtcgatg ccgagccgga tcgtcacttg
     5041 acgagggtgg gggcgaccgg gcccccggcg ccccgggtga agggcagggg cgtggccgtc
     5101 agcaggccgt cgtactggcc gtcggccgca cagtcctcgg ccagggcgtc gaggtaccag
     5161 agctggcctt gcagcaggcc catgtcccgt agctgcagga gatggacggg caggagcacg
     5221 gtgggatcct cgcagggcca acactcgaag acgagcgtgt cggtggcgat ggcgcccacg
     5281 tcgtggtccc gcagccatgc gaccgagcgg gtcgacagcc ccgggctggg gtcggcgaag
     5341 cgcagcttgt cgccccgggc caggaacgcc atctggccgg tgcggaccat gaggacgtca
     5401 ccgggctgga cctcgacgtt cgccagggcg acggcggcgt ccaggtcgtc ggcggtgatg
     5461 gggtggccgt cgtcgaaggt ctcgacgccg agggcccggg cgacgtccaa cagcacaccc
     5521 cgactgacca gtggcccgac cgtgtcgatg ccgaggcggt ggcacccgtc ctcgtcgacc
     5581 gcggagaagg ggacgccgtt gtagagccgg ccctcgtaac cgacgtgggc cagggagtcc
     5641 cagtgggtgg cggcctggat gcccatgctc acccggtcgt cggaggtgca gaagtcgtcg
     5701 gtgtcgcccg tgtacgactg gttgacggcg atcatctccc gctgggggtt gtcccggccc
     5761 gggatgaggc cgatctgggg gccgtcctcg tcgaacggga tcgccagcga gaagggtcgg
     5821 ccctgcttgg ccgcggccac gccccgcagc acggcggtct ggtcgatgag gttgagcgtg
     5881 ccccgctggt cgtcctcccc ccaccgcccc cagttggaga cacgggcggc cagttccttg
     5941 aggtcgggcg ggagtgccat ggtcacccag cctcgcgcgt gcatgatgcg ggccatggac
     6001 agcacgcccg acgaccccgc cgcccaactg gccgacgagc tccggggcgc catcgaggag
     6061 ctgagctcgg tggcccacga ccccgagcgg gtggcccagg cggtgacgat ggcggccgac
     6121 ctgcgc
//
LOCUS       DASTFV010000314         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1181806, whole genome shotgun sequence.
ACCESSION   DASTFV010000314 DASTFV010000000
VERSION     DASTFV010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181806"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1915
                     /locus_tag="VFP02_05915"
     CDS             <1..1915
                     /locus_tag="VFP02_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET9542594.1"
                     /translation="QLAEQFGLSLSELCDEIDVLVHGTTTADNTMIEMDGAATGLLVT
                     EGHRDEIEMRRVHKEQIWDPAAPGPVPIAKRRARIPIPERVDFEGKVLLDLDEEAVRR
                     GVQRLKALGVESIAVMFLFSFVNPDNERRAAEIIREEFPDVAHVSLSHEVRPRGPEYE
                     RVSTTLVNAYVAPRIAHYVDNLSATLREAGYRGPLLIMQSTGGVMPPEHLRGRAVSLL
                     GSGPTGGVMGAAVAAERGGEPDFVAIDMGGTSFDICLVRGGRPEIKTDWNWRYRYYIG
                     LPMVDVQSVGAGGGSIARVRQGALLVGPESAGSTPGPACYGRGGDQPTVTDADAVLGY
                     LPTTGFAGGRMALDVDAARAAIDREVAGPLGLDVTDAAWGIERIVNTNMANATRRVLA
                     GYGADPRDLALIAYGGNGAVHAAPIATELGVDRILVPRAAPAFSALGLLVADYVVDIV
                     RSYVTPLSRVDLGRLRDLVRELGDAADKELEPADLAAADVTRQLFVQMAYAGQNFDMS
                     VPCPEGETLDAAGLLDLADRFHDQHKRDRGFAFRNQEPVVRGARLMARGETPKPEQLA
                     PTVAAATVPTASRPVYFGADWTDTPVHAGPAVATGTTIVGPALVEEPFTVVVVPPGWS
                     TTLADHAAYELRR"
BASE COUNT          274 a          787 c          724 g          264 t
ORIGIN      
        1 ccagctggcc gagcagttcg ggctctccct gagtgagctg tgcgacgaga tcgacgtcct
       61 cgtccacggc accacgaccg ccgacaacac gatgatcgag atggacgggg ccgccaccgg
      121 cttgctcgtc accgaggggc accgcgacga gatcgagatg cggcgggtcc acaaggagca
      181 gatctgggac ccggccgccc cggggcccgt ccccatcgcc aagcgccggg cccgcatccc
      241 gatccccgag cgggtcgact tcgaaggcaa ggtcctgctc gacctcgacg aggaggcggt
      301 gcggcgcggc gtgcaacgcc tgaaggcgct gggggtcgag tcgatcgcgg tcatgttcct
      361 gttcagcttc gtgaacccgg acaacgagcg gcgggccgcc gagatcatcc gcgaggagtt
      421 ccccgacgtc gcccacgtct ccctgtccca cgaggtcagg ccccggggcc ccgagtacga
      481 gcgggtctcg accaccctgg tgaacgccta cgtggccccc cgcatcgccc actacgtcga
      541 caacctgtcg gccacgctgc gggaggccgg ctaccggggg ccgctgctga tcatgcagtc
      601 cacgggcggg gtcatgcccc ccgagcacct gcggggccgg gctgtgagcc tgctcggctc
      661 cgggccgacg ggtggggtca tgggcgcggc ggtggccgcc gaacggggcg gcgagcccga
      721 cttcgtggcc atcgacatgg gcggcaccag cttcgacatc tgcctcgtgc ggggcggccg
      781 cccggagatc aagaccgact ggaactggcg ctaccgctac tacatcggct tgccgatggt
      841 cgacgtgcaa agcgtcgggg caggcggcgg ctccatcgcc cgggtccgcc agggggcgct
      901 cctggtcggg ccggagagcg cgggctcgac cccggggccg gcctgctacg ggcgcggtgg
      961 cgatcagccg accgtgaccg acgccgacgc cgtcctcggc tacctgccca cgaccggctt
     1021 cgcgggcggg cgcatggccc tcgacgtcga cgccgcccgg gccgccatcg accgggaggt
     1081 ggccggaccg ctgggtctcg acgtgaccga cgccgcctgg ggcatcgagc gcatcgtcaa
     1141 caccaacatg gccaacgcca cccgccgggt gctggcgggc tacggcgccg atccccgcga
     1201 cctcgccctc atcgcctacg gcggcaacgg cgcggtgcac gccgcgccca tcgccaccga
     1261 gctgggggtc gaccgcatcc tcgtgccgcg ggccgccccg gccttctcgg ccctcggcct
     1321 gctcgtcgcc gactacgtgg tcgacatcgt ccgctcctac gtgacgcccc tctcgcgcgt
     1381 cgacctcggc cggctacggg acctcgtgcg ggagctgggg gacgcggccg acaaggagct
     1441 cgagccggcc gacctggcgg ccgcggacgt gacccgccag ctgttcgtgc agatggcgta
     1501 cgccgggcag aacttcgaca tgtccgtgcc ctgccccgag ggcgagaccc tcgacgccgc
     1561 cggcctgctc gacctggccg accggttcca cgaccagcac aagcgggacc ggggcttcgc
     1621 cttccgcaac caggagccgg tggtccgcgg ggcccgcctg atggcccggg gcgagacccc
     1681 gaagcccgag cagctggccc ccaccgtcgc cgccgcgacc gtgcctacgg cgagccggcc
     1741 cgtgtacttc ggcgccgact ggaccgacac gccggtgcac gccgggccgg cggtggccac
     1801 cggcacgacg atcgtcgggc ctgcgctcgt cgaggagccc ttcaccgtgg tcgtcgtgcc
     1861 ccccggctgg tcgaccaccc tggccgacca cgccgcctac gagctccgcc gctagtcgat
     1921 atcggcgggc gccaccgcgg cggcttccgg caccagccac tgctccgcga gcgccgcgct
     1981 cacgggccga cccagcaggt agccctgccc caggtcactg ccggcggcct tcagggcgtg
     2041 gtgctgttc
//
LOCUS       DASTFV010000315         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1185781, whole genome shotgun sequence.
ACCESSION   DASTFV010000315 DASTFV010000000
VERSION     DASTFV010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185781"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..936
                     /locus_tag="VFP02_05920"
     CDS             163..936
                     /locus_tag="VFP02_05920"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9542595.1"
                     /translation="MPIEIDPVFRALADPTRRHLLDRLHEDNGQTLRGLCAQAAMTRQ
                     AVTQHLGVLEAANLVTTVRRGREKLHYLNPVPLQEIQERWIDKFEPPRLRALSNVKRR
                     AENAMTDKPSYVYVTYIEATPEAVWQALTDADLTGAYWGHRNVSDWKVGSTWIHERLD
                     GSGNDGGGIVEEADPPRRLVLSWGGLEEQSEETPSRVTFAIEPHGAIVRLTVTHEDLA
                     NEIMREAVAGGWPAVMANLKSLLETGHVLPETPWEMPVG"
     gene            complement(982..>2058)
                     /locus_tag="VFP02_05925"
     CDS             complement(982..>2058)
                     /locus_tag="VFP02_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET9542596.1"
                     /translation="DLVAGATPENYEQALDTVLDDEVIDAVIVVSTPTFAAPSAEVAD
                     VVGRVAARTPKPVLGCFLAAPGLPPLLLGDERPGGVPVFASPEPAARALARAADYAAW
                     RRTPPGTIPSLDDLDLTAARAVVDTFLAESPDGGWLAPAAVTAVLAAVGVPMVRDREV
                     TGAESAAQVAADLGFPVALKAAGTDIVHKSDVGGVALDLLSADAVAAAYRSMAERIGP
                     AMTGALVQQMAPPGVETIVGVVQDPLFGPLLMFGMGGTATELLGDRSFRILPVTDDDV
                     AELVRSRRLSPLLFGYRGAPMADVEALEDLLHRVARLAGAVPEVAELDVNPVIVSSGG
                     AVAVDARLLVRPVAAGPPPDLRRL"
BASE COUNT          325 a          739 c          746 g          248 t
ORIGIN      
        1 ccgtcgacgt tgccatggcg cgccacgccg acgtcgagca cgccctcgtg gccggcctca
       61 cgatggccga gcgaaccgac ctcgagtcgc ttctccgcaa gctcctcgtc gccgtggagg
      121 tcggggattg acaggcaagt aaatacctgc ctattgtgag gcgtgcccat cgagatcgac
      181 ccggtcttcc gggcgctggc ggaccccact cgccggcacc tgctggaccg gctccacgag
      241 gacaacggcc agaccttgcg gggcctgtgt gcgcaggccg ccatgacccg tcaggccgtg
      301 acccagcacc tgggtgtcct cgaggccgcc aacctggtga ccaccgtgcg tcggggtcgg
      361 gagaagttgc actacctgaa cccggtcccg ctgcaggaga tccaggaacg gtggatcgac
      421 aagttcgagc cgccccggct ccgggccttg agcaacgtga agcgacgagc ggagaacgcc
      481 atgaccgaca agcccagcta cgtgtacgtc acctacatcg aggccacgcc cgaagcggtg
      541 tggcaggccc tgaccgacgc cgacctcacc ggtgcctact ggggccaccg caacgtctcg
      601 gactggaagg tcggctcgac ctggatccac gagcgcctcg acggctcggg caacgacggc
      661 ggcggcatcg tggaggaggc cgatccgccc cggcgcctcg tcctgtcctg gggcggcctc
      721 gaggagcagt ccgaggagac gccgtcccgg gtcaccttcg ccatcgagcc ccacggcgcc
      781 atcgtccgtc tgacggtgac ccacgaggac ctggccaacg agatcatgcg ggaggccgtc
      841 gccggcggct ggcccgcggt gatggccaac ctcaagtccc tgctggagac cggccacgtc
      901 ctcccggaga ccccgtggga gatgccggtc ggctgacccg gcccgccggt gggcgcggcc
      961 cgggtcgaag gctccgaccg actagagccg gcgcaggtcg ggtgggggac cggcggcaac
     1021 cgggcggacg aggaggcggg cgtcgacggc aacggcgcca cccgacgaga cgatcaccgg
     1081 attgacgtcg agctcggcga cctcggggac ggcgccggcc aggcgggcga cccggtggag
     1141 cagatcctcc agtgcctcga cgtcggccat cggggcgccc cggtagccga agaggagcgg
     1201 cgacaagcgc cgggagcgga ccaactcggc gacgtcgtcg tcggtcacgg gaaggatccg
     1261 gaacgaccgg tcgcccagca gctcggtggc cgtcccgccc atgccgaaca tcagcagtgg
     1321 cccgaagagg gggtcctgga cgacccccac gatggtctcc acaccgggag gcgccatctg
     1381 ctgcacgagg gcgccggtca tggccggccc gatccgctcg gccatggacc ggtaggccgc
     1441 ggccacggcg tcggcggaca ggagatcgag ggccaccccg ccaacgtcgc tcttgtggac
     1501 gatgtcggtg cccgccgcct tgagcgccac ggggaagccg aggtcggcgg ccacctgagc
     1561 agccgactcg gccccggtga cctcccggtc ccggaccatc ggcacgccca cggcggccag
     1621 gacagccgtg accgccgccg gcgccagcca gccgccatcc ggggactcgg cgaggaacgt
     1681 gtcgaccacc gcccgagccg cggtgaggtc gaggtcgtcg agggacggga tcgtcccggg
     1741 cggcgtccgg cgccaggcgg cgtagtccgc ggcccgggcc agggcgcggg cggcgggctc
     1801 gggtgaggcg aagacgggca ccccacccgg tcgctcgtca ccgagcagca ggggcggcag
     1861 tccgggagca gccaggaaac agcccagcac cggcttcggg gtcctcgccg ccacccggcc
     1921 gaccacgtcg gccacctctg cagacggtgc agcgaacgtc ggggtgctga cgacgatcac
     1981 ggcgtcgatg acttcgtcgt ccagcaccgt gtcgagcgcc tgttcgtagt tctccggcgt
     2041 ggcgccggct acgaggtc
//
LOCUS       DASTFV010000316         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1186402, whole genome shotgun sequence.
ACCESSION   DASTFV010000316 DASTFV010000000
VERSION     DASTFV010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186402"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..607)
                     /locus_tag="VFP02_05930"
     CDS             complement(98..607)
                     /locus_tag="VFP02_05930"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HET9542597.1"
                     /translation="MPKPSSLRRRTKAAVGLTLAAGLAATGLTVAVADEGNPPSLATD
                     DQVVEVRAEIASFVAEVDRVDAVTDYAVQDAARRYSIAVYAQALERRATDGGSSILAA
                     IRACESGGDYGAVSSSGTYRGAYQFDAATWRAVGGHGDPAAASPAEQDRRAQMLYDRS
                     GSSPWPVCG"
     gene            complement(794..1930)
                     /locus_tag="VFP02_05935"
     CDS             complement(794..1930)
                     /locus_tag="VFP02_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HET9542598.1"
                     /translation="MSEIEPFDITVPEHVLDDLKDRLGRTRWPDQIPDTGWDYGTDLA
                     YLQELCTYWRDEYDWRAHEATLKSWDHFATEIDGQNIAFIHARSPVEGARPLILSHGW
                     PGSVVEFLHVIDPLRDPAAHGGDPADAFHVVVPSLPGYGWSGPTVDRGWDPKRIALAF
                     AALMDRLGYDGYVAQGGDWGSMISTQIGIQDPDHCAGIHLNMVIALPGPDDELTEEEQ
                     AAMADAIKIMSDGAGYQAIQGTRPQTLGYGLSDSPAGLAGWIAEKFHAWTDNDGTIES
                     AVSRDDLLTNITTYWVTGTINSSTRLYYEAKKSGLFGTQGADVTVPTAGAVFPKEIVR
                     ASRRFAERSYNVVQWSTFDRGGHFAALEEPDLLIDDLRSFSRLL"
     gene            complement(1927..>2529)
                     /locus_tag="VFP02_05940"
     CDS             complement(1927..>2529)
                     /locus_tag="VFP02_05940"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HET9542599.1"
                     /translation="RIQRQDCSQVTDGAACVVLAGERFATAWADSHGRSLDDTGRLAG
                     WGHRTARMALEDKLADSQGSPYVAPFVRQTVVDAFGRAGVADARDLDAVEVHDCFTTT
                     EYLAIDHLGLTAPGESWRAIEDGTIGPDGSVPINPSGGLIGGGHPVGATGVRMVLDAT
                     KQVTGTAGGYQVDGARTVATLNLGGSATTSAAFVLTAGAT"
BASE COUNT          343 a          847 c          955 g          384 t
ORIGIN      
        1 aggtggccct ccagcacaac ctcggcctcg gcggcgccgg tgtggtcacg atgtacagag
       61 ccccggcctg acgcgggccg agaggcgcag aagacagcta cccgcagacg ggccaggggc
      121 tggaaccgct ccggtcgtag agcatctggg ctcgcctgtc ctgctcggcc ggcgaggcgg
      181 ccgccgggtc accgtgaccg ccgaccgccc gccaggtggc ggcgtcgaac tggtacgcac
      241 cgcgataggt cccgctcgag ctgacggcgc cgtagtcacc accggactcg caggcccgga
      301 tggcggccag gatgctgctc ccgccgtcgg tggcccgccg ttcgagggcc tgggcgtaga
      361 cggcgatgct gtagcgccgg gcggcgtcct ggacggcgta gtcggtcacc gcgtcgaccc
      421 ggtcgacctc ggcgacgaac gacgcaatct cagcccggac ctcgacgacc tggtcgtcgg
      481 tggccaacga gggcgggttg ccctcgtcgg cgacggccac ggtgaggccg gtcgcggcca
      541 gaccggcggc gagggtcagg cccaccgccg ccttggtccg ccgtcggagg gatgacggct
      601 tcggcatggt ttcttgctcc tgtgcgtgac agcgcgcgac gaaggaggcg gcactgaaag
      661 ggaggaccgc tcctcagccg ggcgcccgtg cacctggggt gtggggtccc gaagagggtg
      721 cacgccttcg taacgcaaat gcaacatttg acccctgtgt cgtgggtcac ggccccccga
      781 atggggttga cgcctacagc agtcggctga agctgcggag atcgtcgatc agcaggtcgg
      841 gctcttcgag ggcggcgaag tggccgccgc ggtcgaaggt cgaccactga acgacgttgt
      901 agctgcgctc ggcgaagcgg cgggacgccc gcacgatctc cttcgggaac acggcgccgg
      961 cggtggggac ggtgacgtcg gcgccctgcg tcccgaacag gcccgacttc ttggcctcgt
     1021 agtagaggcg ggtcgaggag ttgatggtgc ccgtcaccca gtaggtggtg atgttggtga
     1081 gcaggtcgtc gcgggagacg gcggactcga tggtgccgtc gttgtcggtc caggcgtgga
     1141 acttctcggc gatccagccg gccaggcccg ccggtgagtc gctcaggccg tagcccaggg
     1201 tctggggccg ggtgccctgg atggcctgat agccggcgcc gtcactcatg atcttgatgg
     1261 cgtcggccat cgccgcctgc tcctcctcgg tgagctcgtc gtcgggcccg ggcagcgcga
     1321 tcaccatgtt caggtggatg ccggcgcagt ggtcggggtc ctggatgccg atctgggtgg
     1381 agatcatcga gccccagtcc ccgccctggg cgacgtagcc gtcgtaaccc agccggtcca
     1441 tgagcgcggc gaaggccagg gcgatgcgct tggggtccca gccccggtcc accgtcggcc
     1501 ccgaccagcc gtagccgggc agcgacggga caacgacgtg gaaggcgtcg gcggggtccc
     1561 cgccgtgggc tgcggggtcc cgcaggggat cgatgacgtg caggaactcg acgaccgacc
     1621 cgggccaccc gtgactgagg atcaggggcc gggccccctc gaccggcgat cgggcgtgga
     1681 tgaaggcgat gttctgcccg tcgatctcgg tggcgaagtg gtcccaggac ttgagggtgg
     1741 cctcgtgggc ccgccagtcg tactcgtccc gccagtaggt gcacagctcc tgcaggtagg
     1801 ccaggtcggt gccgtagtcc cagccggtgt ccgggatctg gtcgggccag cgcgtccggc
     1861 ccaggcggtc cttcaggtcg tccaggacgt gctcggggac ggtgatgtcg aagggctcga
     1921 tctcgctcat gtcgctcctg cggtgaggac gaaggcggcg ctggtggtgg cgctgccgcc
     1981 gaggttgagg gtggcgacgg tgcgggcccc gtcgacctgg tagccgccgg ccgtcccggt
     2041 cacctgcttg gtggcgtcga ggaccatgcg cacaccggtc gccccgaccg ggtggccgcc
     2101 gccgatgagg ccaccgctcg ggttgatcgg gacgctcccg tcggggccga tcgtgccgtc
     2161 ctcgatggcc cgccagctct cccccggcgc cgtcagcccc aggtggtcga tggccaggta
     2221 ctcggtggtg gtgaagcagt cgtggacctc gacggcgtcc aggtcacggg cgtcggccac
     2281 gccggcccgc ccgaacgcgt cgaccacggt ctgacgcacg aacggggcga cgtaggggct
     2341 gccctggctg tcggccagct tgtcctcgag cgccatgcgg gccgtgcggt ggccccagcc
     2401 cgccaggcga ccggtgtcgt ccaacgaccg gccatggcta tcggcccacg ccgtggcgaa
     2461 gcgctcccca gccagcacga cgcaggccgc cccgtcagtg acctgcgagc agtcctggcg
     2521 ttggatgcg
//
LOCUS       DASTFV010000317         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1188037, whole genome shotgun sequence.
ACCESSION   DASTFV010000317 DASTFV010000000
VERSION     DASTFV010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188037"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFP02_05945"
     CDS             complement(<1..209)
                     /locus_tag="VFP02_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542600.1"
                     /translation="MRGKGALVAFGVWTLFVWATRIGTAWTQDDLSAAARAGRVGLAL
                     SFTVLGVALLIVAGRSRGRALTRAEA"
     gene            273..1535
                     /locus_tag="VFP02_05950"
     CDS             273..1535
                     /locus_tag="VFP02_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9542601.1"
                     /translation="MAEPTLDEFRDEVTAFLDANAERRAEVKGDQKFVWGEGDDDVAI
                     LEETSREQELADLAEAKVWKAKRYDAGFGWITGPTRYGGRELPVAYDRAYASLESNYQ
                     IPSQSHFGIGLGMVAPTILAHATEKVKDLYLAKLYRGDLVASQLFSEPGAGSDLAGLQ
                     TKAVKDGDEWIVNGQKVWTSGAHYSDIGEVICRTDPDQPKHRGLTGFVVDLKAPGVEI
                     RPLRQMTGGASFNEVFFTDVRIPDDHRLGDVGQGWAVALTTLMNERASIGAGSGSGMG
                     LGNATRLIEMVRHFGLDEDPLTRGELARLYENLQVAKWNNQRAMDKIKSGQAPGPEMS
                     IAKMALTRNMQMTGDFVSKVLGPRMVADTREWGTYAWGKFVLGTPGMRVAGGTDEVLR
                     NIVSERVLGLPKEPGIDTTSPFKDLAQN"
     gene            complement(1532..>2227)
                     /locus_tag="VFP02_05955"
     CDS             complement(1532..>2227)
                     /locus_tag="VFP02_05955"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET9542602.1"
                     /translation="VTAPQLRDALKRGSPSLSVERDLWAAGHEVIVGVDEVGRGAWAG
                     PISVGAAVLPQDRRVYKVRDSKMLTEPEREVMFDRIAEWCVTWAVGHASQEECDTLGM
                     SAAQKLAAARAIDGLGVEPDRVLIDGNWDFVGGGRRTRRIVRGDATCLSIAAASILAK
                     VTRDRIMRAEAPHFPGYDFEFNKGYPCPRHKAALQWIGPSAIHRRSWVFMDHLSWTGI
                     PRTIPPSMQGELF"
BASE COUNT          374 a          798 c          761 g          294 t
ORIGIN      
        1 gcttccgccc gggtcagggc ccgcccccgc gaacgtccgg ccacgatcag gagggccacc
       61 ccgagcaccg tgaacgagag ggccaggccg acgcggccgg cccgggccgc ggcgctcaga
      121 tcgtcctggg tccaggcggt gccgatccgc gtggcccaga cgaacagggt ccacaccccg
      181 aaggcgacca gtgcgccctt gcctcgcatg ggaccgacgg taccggcgcg gctagcttga
      241 ccagtcagtc aagtttcctc cgggagcgca ccatggcaga acccacgctg gacgagttcc
      301 gagacgaggt caccgccttc ctcgacgcca atgccgagcg ccgggccgag gtcaagggcg
      361 accagaagtt cgtctggggc gagggcgacg acgacgtcgc catcctcgag gagacgagcc
      421 gggagcagga gctggccgac ctggccgagg ccaaggtctg gaaggccaag cgctacgacg
      481 ccggcttcgg ctggatcacc ggccccaccc gatacggcgg ccgggagctg ccggtggcct
      541 acgaccgggc ctacgcctcg ctggagagca actaccagat ccccagccag agccacttcg
      601 gcatcggcct cggcatggtc gcgcccacga tcctggccca cgccaccgag aaggtgaagg
      661 acctctacct ggccaagctc tatcgggggg acctggtcgc ctcgcagctg ttcagcgagc
      721 ccggtgccgg ctccgacctc gccggtctgc agaccaaggc cgtcaaggac ggcgacgagt
      781 ggatcgtgaa cggccagaag gtctggacct ccggcgccca ctacagcgac atcggcgagg
      841 tcatctgccg caccgacccc gatcagccca agcaccgggg cctcaccggg ttcgtcgtcg
      901 acctgaaggc gcccggcgtc gagatccgtc cgctgcgcca gatgaccgga ggcgcctcgt
      961 tcaacgaggt cttcttcacc gacgtccgca tccctgacga ccaccgcctc ggcgatgtcg
     1021 gccagggctg ggccgtcgcc ctcacgacgc tcatgaacga gcgggcctcc atcggggccg
     1081 gcagcggctc gggcatgggc ctcggcaacg ccacgcggct gatcgagatg gtccgccact
     1141 tcggactcga cgaggacccc ctgacccggg gcgagctggc ccggctctac gagaacctcc
     1201 aggtggcgaa gtggaacaac cagcgggcca tggacaagat caagtccggc caggcgcctg
     1261 gccccgagat gtccatcgcc aagatggccc tgacccgcaa catgcagatg acgggcgact
     1321 tcgtctcgaa ggtgctcggc cccaggatgg tggccgacac ccgcgagtgg ggcacttacg
     1381 cctggggcaa gttcgtcctc ggcacaccgg gcatgcgggt ggccggtggc accgacgagg
     1441 tcctgcgcaa catcgtcagc gagcgggtcc tcggcctgcc caaggagccg ggcatcgaca
     1501 cgaccagccc cttcaaggac ctggcccaga actagaacag ctcgccctgc atggacgggg
     1561 ggatcgtgcg ggggatgccg gtccagctga ggtggtccat gaagacccag gagcgccggt
     1621 ggatggcgct ggggccgatc cactgcaggg ctgccttgtg gcgggggcag ggatagccct
     1681 tgttgaactc gaagtcgtag cccgggaagt ggggggcctc ggcccgcatg atccggtccc
     1741 gggtgacctt ggccaggatc gacgccgccg cgatcgacag gcacgtggcg tcgccccgca
     1801 cgatgcgccg ggtgcggcga cccccaccca cgaagtccca gttcccgtcg atgaggaccc
     1861 gatcgggctc gacgccgagc ccgtcgatgg cccgggccgc cgccagcttc tgggcggcgg
     1921 acatgccgag ggtgtcgcac tcctcctggc tggcgtgacc cacggcccag gtgacacacc
     1981 actcggcgat gcggtcgaac atgacctcgc gctcgggctc ggtcagcatc ttggagtccc
     2041 ggaccttgta gacgcgccgg tcctggggca ggacggcggc gcccacgctg atcggccccg
     2101 cccacgcccc ccggccgacc tcgtccacgc ccacgatgac ctcatggccc gccgcccaca
     2161 ggtctcgctc gactgacagc gacggtgaac cgcgcttcaa tgcgtcacgg agttgcggag
     2221 cggtgac
//
LOCUS       DASTFV010000318         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1188436, whole genome shotgun sequence.
ACCESSION   DASTFV010000318 DASTFV010000000
VERSION     DASTFV010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188436"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFP02_05960"
     CDS             <1..470
                     /locus_tag="VFP02_05960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9542603.1"
                     /translation="DGRQIEDQPLPRGYAIRSATEDDHRAAWTVIEDAFLEWSDRDRQ
                     PFEDFAAQVMRRPGFQPWNLRVVTDAADQIVGAAFVILNGDVGYVEKVAVKPAQRGRG
                     LARALLADAFALSRAHGGTASMLSTDSRTGALSLYEKVGMEVVAVWLHRSIAL"
     gene            550..1200
                     /locus_tag="VFP02_05965"
     CDS             550..1200
                     /locus_tag="VFP02_05965"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HET9542604.1"
                     /translation="MTALPAGWYGDPADSTRYRYWDGEQWTTHVQNRAEVDIAAAEAD
                     MAAAARPLPDFDDPDGTASGATTGRRRVPILLAVVALAAIAGAAAFVVTSGGGDSGQT
                     LAGEVRVGTASVRGGGEPDDGAAGDGCGTGSAQSIGAGSALTVSNARGEVLGRTELQA
                     GRLDRSPTATTCVFPYEVTGVDDAAVYLVQVAERRPTRSTRAQVEADRGRIDVLLR"
     gene            complement(1247..>2146)
                     /locus_tag="VFP02_05970"
     CDS             complement(1247..>2146)
                     /locus_tag="VFP02_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9542605.1"
                     /translation="EDEPWEGERLIWPPYAVGGVSQGHLSEAEARHLRANYGAKVSMI
                     DHWFGRVLAALDDQGLWDSTAVVVCTDHGHYLGDVRGGDDLWGKPGVPQYEPLGHTPL
                     LVHWPGVGGGGTCDALTTSVDLHATLSEVFGVSAGHRTHGRSLAPLLTGEATAVRAWA
                     IGGVWGNWVQVTDGHRKYARAPVGDPFPLSMWSNRWSTMPLHIAGVEGLPPPDGRAFL
                     DTMPGTDIPVIRQPYAAGDRLPFWAGQGQVDQHHLYDLDLDPDEAENRTGEAVEADLV
                     DLLRSALVELEAPTEQLERLGLA"
BASE COUNT          291 a          796 c          770 g          289 t
ORIGIN      
        1 ccgacggtcg ccagatcgag gaccagcccc ttccccgcgg atacgccatt cggtccgcca
       61 ccgaggacga ccaccgggcg gcctggaccg tcatcgagga cgccttcctc gagtggtccg
      121 accgggaccg acagccgttc gaggacttcg ccgcccaggt catgcgccgt ccgggcttcc
      181 agccctggaa cctccgtgtc gtcaccgacg ccgccgacca gatcgtggga gcggctttcg
      241 tcatcctcaa cggcgacgtg ggttacgtcg agaaggtggc cgtcaagccc gcacagcggg
      301 gccggggtct cgcccgggct ctgctggccg acgcctttgc gctgtcccga gcccacgggg
      361 gcaccgcgtc gatgctgtcg accgactccc ggacgggcgc cctcagcctg tacgagaagg
      421 tcggcatgga ggtcgtcgcc gtctggttgc accgctcgat cgccctctga gccaggggct
      481 ggcgctcacc tccgcccgcc aagtgccgac ggtcaaccag gtaggtcctc gaagcacgaa
      541 aggcgcgcgg tgactgcact ccccgcgggt tggtacggcg atccggccga ctccacccgc
      601 taccggtact gggacggcga gcagtggacg acccacgtcc agaaccgggc cgaggtcgac
      661 atcgccgccg ccgaagccga catggccgcc gcggcccggc cgctccccga cttcgacgac
      721 ccggacggca cggcctccgg agcgaccact ggccggcgcc gcgtccccat ccttctcgcc
      781 gtggtcgccc tggccgccat cgccggggcc gcggccttcg tcgtcacctc cggtggcggc
      841 gacagcggtc agaccctcgc cggtgaggtc cgcgtcggca ccgcgtcggt ccgcggtggc
      901 ggagagcccg acgacggtgc cgcgggcgac ggctgtggga cgggatcggc gcagagcatc
      961 ggagccgggt cggcgctcac cgtgagcaac gcccggggcg aggtcctcgg ccgcaccgag
     1021 ttgcaggccg gtcgcctcga ccggagtccc accgccacga cctgcgtgtt cccctatgag
     1081 gtcacgggcg tcgacgacgc cgccgtctac ctggtgcagg tcgccgaacg gcggccgacc
     1141 cgctcgaccc gggcccaggt cgaggccgac cgcggccgca tcgacgtcct gctgcgctga
     1201 gcgttctcgg aagtgcccgg gcgattcgcc agggcttccc ggtcgatcac gccaggccga
     1261 ggcgttcgag ctgctcggtc ggcgcctcca gctcgaccag ggccgagcgc aagaggtcga
     1321 ccaggtcggc ctccacggcc tcgccggtcc ggttctcggc ctcgtcgggg tcgaggtcga
     1381 ggtcgtagag gtggtgctgg tcgacctggc cctggccggc ccagaacggg aggcggtcac
     1441 cggcggcgta gggctggcgg atgaccggga tgtccgtgcc gggcatggtg tccaggaagg
     1501 cccggccgtc gggcgggggc aggccctcca ccccggcaat gtgcaggggc atggtggacc
     1561 agcggttgga ccacatcgac agggggaagg ggtcgccgac cggggcgcgg gcgtacttgc
     1621 ggtggccgtc ggtgacctgg acccagttgc cccagacgcc gccgatggcc cacgcccgca
     1681 ccgccgtggc ctcgcccgtc aacagcggcg ccaacgaccg accgtgggtg cggtggccgg
     1741 cgctcacccc gaacacctca ctcagggtgg cgtgcaggtc gacgctggtc gtcagcgcgt
     1801 cacaggtccc gccgccgcca acccccggcc agtgcacgag cagcggggtg tgacccagcg
     1861 gctcgtactg aggcacgccc ggcttgcccc agaggtcgtc gccgccgcgg acgtcaccga
     1921 ggtagtggcc gtggtcggtg cagaccacca ccgccgtcga gtcccacagg ccctggtcgt
     1981 ccagggcggc gaggacccga ccgaaccagt ggtcgatcat ggagaccttg gcgccgtagt
     2041 tggcccgcag gtgacgggcc tcggcctcgc tcaagtggcc ctggctgacc ccgccgacgg
     2101 catagggcgg ccagatcagg cgctcgccct cccagggctc gtcctc
//
LOCUS       DASTFV010000319         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1191887, whole genome shotgun sequence.
ACCESSION   DASTFV010000319 DASTFV010000000
VERSION     DASTFV010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191887"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VFP02_05975"
     CDS             complement(<1..591)
                     /locus_tag="VFP02_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9542606.1"
                     /translation="MAQLIAAGVHEIDTLLGGWERVTAGYLVDGDEPVLVETGSQSSV
                     PALLAALDALGIAPDALAHVVVTHIHLDHAGGVGDVARAFPMATIHVHEKGARHLIDP
                     RRLVDSAAMVYGDLLDSLYGRLTPTDAARVHVLADGEELRVSPTRVLTTIDSPGHAKH
                     HLGLHDSVSGLLFVGDAVGVRLPDAGILRPATPPPDF"
     gene            634..1905
                     /gene="xseA"
                     /locus_tag="VFP02_05980"
     CDS             634..1905
                     /gene="xseA"
                     /locus_tag="VFP02_05980"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET9542607.1"
                     /translation="MEDRLFDPTPTLSVSEVLSRARVVIERAFGDEVWIEGEIQNLPE
                     NRAPDTHLYFDIVETRPDGRVAGRLPVVLFAQNRRGVNVLLKRSGGKLRMADGLNVRL
                     RGQVTAYERTGQVQLRMTAIDPNHTIGLLAAERDRVMETLVREGLLAVNGRRPIVGPP
                     LRIALLTSQGSAAAEDFLHELSVSGIGFDITLHDVRVQGREAEPTIVAALTDVDASTT
                     DVVALVRGGGARTDLAAFDAEGIARAIAGLPVPLITGLGHEIDRAVADEVAHTSLKTP
                     TACAAWLVDRVNAWLVDLDDAWATVADRARLHLDRADQRVHRAARRAVIASRRSLDRA
                     DRHHRDLAGEIRRRSPRTLDRADQRLLQWAARINAYDPARSLARGWSITRTATGALVR
                     SPADAPPGTVLVTTLAEGELASRAENPEDPA"
     gene            1902..2120
                     /gene="xseB"
                     /locus_tag="VFP02_05985"
     CDS             1902..2120
                     /gene="xseB"
                     /locus_tag="VFP02_05985"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET9542608.1"
                     /translation="MTTDPAEPGYAEALTELEAILDELDDDRLDVDALAERVQRAAHL
                     VALCRERVAGARLAVEEVVTDLDGDDPD"
     gene            2166..>2529
                     /locus_tag="VFP02_05990"
     CDS             2166..>2529
                     /locus_tag="VFP02_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542609.1"
                     /translation="MILRDLPEDGSRAATGPYALRSRHTGRLIARIDGDLHLVFVDPG
                     FLNFFGYTNLDGDDPAVLSRLGRGIDHDAEVVARWRDVVARPGATDRIDIRLCAADGT
                     WRWARMDLENRLDDPEDPY"
BASE COUNT          379 a          933 c          883 g          334 t
ORIGIN      
        1 gaagtcggga ggaggcgtgg ccggacgcag gatgccggcg tcgggcaggc gcacgccaac
       61 tgcgtcaccg acgaagagca ggccgctcac cgagtcgtgc aggccgagat ggtgcttcgc
      121 atgacccggt gagtcgatgg tcgtcagcac acgggtcggg ctgacgcgca gctcctcgcc
      181 gtccgccagg acatggactc gggctgcgtc ggtgggcgtg aggcggccgt agagcgagtc
      241 caacaggtcc ccgtagacca ttgccgcgga gtcgaccagg cggcggggat cgatcaagtg
      301 gcgagccccc ttctcgtgga cgtggatcgt ggccatgggg aaggcccggg ccacgtcccc
      361 cacgccgccg gcgtgatcga ggtggatgtg ggtgaccacg acatgggcca gggcgtcggg
      421 ggcgatgccg agggcgtcca gggccgcgag gagcgccggg accgacgact ggctaccggt
      481 ctcgaccagg accggctcgt ccccgtcgac caggtacccc gccgtgaccc gctcccagcc
      541 acccagcagc gtgtcgatct cgtgcacacc cgcggcgatc agctgcgcca tgggagcaac
      601 gttgtcacac ctcggaagta ggttcggcgg cgcatggagg accgcttgtt cgacccgacc
      661 ccgaccctgt cggtgagcga ggtgttgagc cgggcgcgag tggtcatcga gcgggccttc
      721 ggcgatgagg tgtggatcga gggcgagatc cagaacctcc ccgagaaccg ggcaccggac
      781 acgcacctct acttcgacat cgtcgagacg cggcccgacg gtcgggtcgc cggacgcctg
      841 ccggtcgtcc tcttcgccca gaaccgacgc ggcgtcaacg tcctgctcaa gcgctcgggc
      901 ggcaagctgc gcatggccga cgggctgaac gtccggctgc ggggccaggt caccgcctac
      961 gaacgcaccg gccaggtcca gctgcgcatg accgccatcg accccaacca caccatcggc
     1021 ctactcgccg cagaacgcga ccgcgtgatg gagacgctgg tccgcgaggg cctgctcgcg
     1081 gtgaacggtc ggcgcccgat cgtcggtccg ccactgcgga tcgccctgct gaccagccag
     1141 ggcagcgccg cggccgagga cttcctgcac gagctgtcag tcagcgggat cggcttcgac
     1201 atcaccctcc acgatgtccg ggtacagggc cgggaagccg agccgacgat cgtggcggcc
     1261 ctgaccgacg tcgacgcgtc caccaccgac gtcgtcgccc tggtgcgcgg tggcggggcc
     1321 cgcacggacc tggccgcctt cgacgccgag ggcatcgccc gggccatcgc cgggcttccg
     1381 gtgcccctga tcaccggcct cggccacgag atcgaccggg cggtggccga cgaggtggcc
     1441 cacaccagcc tgaagacgcc gacggcctgc gcggcttggc tggtcgaccg ggtcaacgcc
     1501 tggttggtcg acctggacga cgcgtgggcc acagtggccg accgggcacg gctgcacctc
     1561 gaccgggccg accagcgggt gcaccgggcc gcccgccggg cggtcatcgc cagccgccgg
     1621 agcctcgacc gagccgaccg gcaccatcgc gacctggccg gcgagatccg ccgccgcagc
     1681 ccccgcacgt tggaccgggc cgaccaacgc ctgctgcagt gggccgcccg gatcaacgcc
     1741 tacgaccccg cccgctcact ggcccggggg tggtccatca cccggaccgc caccggcgct
     1801 ctcgtccgct cccccgccga cgcaccccct ggcactgtgc tggtcaccac cctcgccgaa
     1861 ggcgagctgg ccagccgggc cgagaaccca gaggaccccg catgaccacc gatcccgcag
     1921 aaccgggcta cgccgaggcg ctgaccgagc tcgaggccat tctcgacgaa ctggacgacg
     1981 accgcctcga tgtcgacgcc ttggccgagc gcgttcagcg ggcggcccac ctcgtggctc
     2041 tctgccggga gcgcgtggcc ggcgcccgcc tcgccgtcga ggaggtcgtc acggacctgg
     2101 acggggacga tccggactga ccgtttgcca cggggtgcgg tcacgtgact acactgcgta
     2161 ctcgcgtgat cctgcgtgac ctccccgagg acggctcccg ggcggcgact ggtccctacg
     2221 cgctccggtc tcgtcacacg gggcgtctga tcgcccgcat cgacggcgat ctgcacctcg
     2281 tcttcgtcga ccccgggttc ctcaacttct tcggctacac gaacctcgac ggcgacgacc
     2341 ctgccgtcct gagccggctc ggtcggggca tagaccacga cgccgaggtg gtggcgcggt
     2401 ggcgtgacgt cgtggcccgc cccggggcca ccgaccggat cgacatccga ctctgtgcgg
     2461 ccgacgggac ctggcgctgg gcccggatgg acctcgagaa ccgcctggac gaccccgagg
     2521 atccctacc
//
LOCUS       DASTFV010000320         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1195206, whole genome shotgun sequence.
ACCESSION   DASTFV010000320 DASTFV010000000
VERSION     DASTFV010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195206"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /gene="galT"
                     /locus_tag="VFP02_05995"
     CDS             <1..1053
                     /gene="galT"
                     /locus_tag="VFP02_05995"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HET9542610.1"
                     /translation="ASLRSARNEAVLIGRLAWRLGSAVTWELRWHPFRGEWVLFTAHR
                     GARPWIGEVVEDEEPSVPEDNALAPRGKRIATTNPDYRGVFTFTNDLPVFSQEAPEPS
                     PGDELYRTQRAVGTAEVVCYHHDPTKSMADLDDDELTAVVTTWRDRTEALEALDGVAH
                     VLIFENRGAAVGTSNPHPHCQIYAGAMVYATMAREAEVAAEHHGRTGRTLLEDVTERE
                     RAGPRVISEGETFFACVPWFARYAYEVHVLPRRPMASLVALDDAGCRSLGLVLRDVVR
                     RYDALWGLRMPYVLAVHQAPVGDHPHYPFHVELHPPLRAPGLLKYLAGPEVGGGSMTN
                     ESDPDEKAAELRDALD"
     gene            1037..1960
                     /locus_tag="VFP02_06000"
     CDS             1037..1960
                     /locus_tag="VFP02_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET9542611.1"
                     /translation="MTHSTDDARRLLCELGNDLRDMVIGARGMDMSAVEGQTSADTIY
                     AIDRVVDDALLGWFEQHWPGVEVVSEGLEEPVVVGGAPTWTVIVDTIDGTRGLMYDKR
                     PAWCLAGAAPHGGSLADIVASAMTELPTVKQGAADQLSGVRGGGVVAERVDLRTGDRE
                     PTTVRPSSATDLEHGFGGLAKFFVPGKADLAALEAELFRRLGSRDVFDDEYISSGGQL
                     HELLTGRDRFVADLRPLIAPGAHACHPYDVCTAMLLEEAGGVVTDPWGGPLDAPLDTT
                     SPIAWVGYANPTLAASIGPVLAELVDEHRPR"
     gene            1941..>2311
                     /locus_tag="VFP02_06005"
     CDS             1941..>2311
                     /locus_tag="VFP02_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542612.1"
                     /translation="MSTVRGSAPGRLDLLGGVADYSGALVLETPTRLVTTVVAEPADA
                     LVVGPVHLAEDEVTKLAGLTYPELRDALADLPKWTHYLLGVALVLVRHGVIEPPRAQL
                     SVSSDLPVSVGVSSSAALEVAT"
BASE COUNT          307 a          843 c          820 g          341 t
ORIGIN      
        1 gcctcgctgc gctcggcgag aaacgaggct gtgctcattg gtcgcctcgc ctggcggctc
       61 ggctccgctg tgacctggga gctgcggtgg cacccgttcc gcggggaatg ggtcctcttc
      121 accgcgcatc ggggggctcg tccctggatc ggcgaggtgg tggaggacga ggaaccgtcg
      181 gtacccgagg acaacgccct cgcgcctcgg ggcaaacgca tcgccaccac caaccccgac
      241 taccggggcg tgttcacgtt caccaacgac ctcccggtgt tctcgcagga ggcgccggaa
      301 ccgtcgccgg gagacgagct ctaccggacg cagcgggcgg tggggacggc cgaggtcgtc
      361 tgttaccacc acgaccccac gaagtcgatg gccgacctcg acgacgacga gctgacggcg
      421 gtggtgacca cgtggcggga ccggaccgag gcgctcgagg ccctcgacgg cgtcgcccac
      481 gtgctcatct tcgagaaccg gggggcggcg gtgggcacgt cgaaccccca cccgcactgt
      541 cagatctatg ccggcgcgat ggtgtacgcc acgatggccc gtgaggccga ggtggcggcc
      601 gagcaccatg gccggaccgg gcgcaccctg ctggaggacg tcaccgagcg cgagcgggcg
      661 ggaccgcggg tcatcagcga gggcgagacg ttcttcgcct gtgtcccgtg gttcgcccgg
      721 tatgcctacg aggtgcacgt cctgccccgg cggccgatgg cgtcgctggt cgccctcgac
      781 gacgctgggt gccgctccct cggcttggtc ctgcgggacg tggtccggcg ttacgacgcg
      841 ctgtggggcc tgcgcatgcc ttacgtcctc gccgtccacc aggcgccggt cggcgatcac
      901 ccgcactacc ccttccacgt cgagctccat ccgcccctgc gggcgccggg gctgctcaag
      961 tacctggccg gcccggaggt cggtggcggc tcgatgacca acgaatccga ccccgacgag
     1021 aaggcggcag agctccgtga cgcactcgac tgacgacgcc cgccggctcc tctgtgagct
     1081 cggcaacgac ctgcgggaca tggtcatcgg ggccaggggc atggacatgt ccgccgtcga
     1141 gggccagacc tcggccgaca cgatctatgc catcgaccgg gtggtcgacg acgcgctgct
     1201 cggctggttc gagcagcact ggccgggcgt ggaggtggtg tccgaggggc tcgaggagcc
     1261 cgtcgtcgtc ggcggtgccc cgacctggac cgtgatcgtc gacaccatcg acggcacgcg
     1321 cgggctcatg tacgacaagc gccccgcgtg gtgcctcgcc ggcgccgccc cccacggggg
     1381 atccctggcc gacatcgtgg catcggcgat gaccgagctg cccacggtca agcagggcgc
     1441 ggccgaccag ctgagcgggg tccggggcgg tggtgtcgtg gccgagcgag tcgatctccg
     1501 cacgggcgac cgggagccga cgaccgttcg cccgtcgtcg gccaccgatc tcgagcacgg
     1561 tttcggcggc ctggccaagt tcttcgtgcc gggcaaggcc gacctcgccg cgctggaggc
     1621 ggagctgttc cggcgcctcg gcagccggga cgtcttcgac gacgagtaca tctcttccgg
     1681 cggccagctc cacgagctcc tgaccgggcg ggaccgcttc gtcgccgacc tccgccccct
     1741 catcgccccc ggggcccacg cctgccaccc ctacgacgtc tgcacggcga tgctgctcga
     1801 agaggccggg ggtgtcgtca ccgacccctg gggcggaccg ctggatgcgc ccctggacac
     1861 gacgtcaccg atcgcctggg tcggctacgc caaccccacc ctggccgcga gcatcggtcc
     1921 cgttctcgcc gagctcgtcg atgagcaccg tccgcggtag cgccccgggt cgcctcgacc
     1981 tcctcggggg cgtggccgac tactcggggg cgttggtgct cgagacaccg acccggctgg
     2041 tgaccaccgt cgtggccgag ccggccgacg cgctggtggt cggccccgtc cacctcgccg
     2101 aggacgaggt cacgaagctg gccggtctga cctacccgga gctgcgcgac gccctggccg
     2161 atctgccgaa gtggacccac tacctcctcg gcgtcgcgct cgtcctggtg cgccacgggg
     2221 tgatcgagcc gccccgagcg cagctgtcgg tctcgtcgga ccttcccgtg tcggtcggcg
     2281 tctcgtccag cgccgcgctc gaggtggcca c
//
LOCUS       DASTFV010000321         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1195500, whole genome shotgun sequence.
ACCESSION   DASTFV010000321 DASTFV010000000
VERSION     DASTFV010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195500"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..593)
                     /locus_tag="VFP02_06010"
     CDS             complement(93..593)
                     /locus_tag="VFP02_06010"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HET9542613.1"
                     /translation="MALRPTRIPPGPGQESVWDYPRPPRIEPTDRRVRVELGGVLIGD
                     TTRALRVLETSQPPAFYLPPEDVAREHLERTDKHSFCEWKGHADYWTVTVGDRVARDA
                     AWSYPAPNPAFAAIADHLAFYPRAMDGCFVDDERVESNDGDFYGGWITSAVVGPFKGA
                     PGTGHW"
     gene            612..1088
                     /locus_tag="VFP02_06015"
     CDS             612..1088
                     /locus_tag="VFP02_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542614.1"
                     /translation="MEEDMDRALAEKAAALADAIDAAVTPWLIGAVRRVAASQRLPGG
                     DRFVLAAETAANQAKAEVMPRIRDLLTTDIDAQTTTPLAVLREGTGPATAVLAEFGAR
                     PVERDEFAVRAFPDDVYALAPAAFEDVAPELRDPGIEWGAAKAFVHLRRRRAEGVV"
     gene            complement(1085..1600)
                     /locus_tag="VFP02_06020"
     CDS             complement(1085..1600)
                     /locus_tag="VFP02_06020"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HET9542615.1"
                     /translation="MVFGFGGGPIRRERDGTFTIRLSEPEQVLLKSLLAQLRTLLKLE
                     GGSEITDESGVRRLFPTAYAGRDDLEAEYQAMVHDDLLARRLANIDEIESSIVLPSLD
                     EEQLGTWLRTFNDLRLVLGTRLDVSEDDLGPIDPDDPEAPAQAAYEYLGWLLEATVSA
                     LTGSLPDPEDD"
     gene            complement(1600..1896)
                     /gene="clpS"
                     /locus_tag="VFP02_06025"
     CDS             complement(1600..1896)
                     /gene="clpS"
                     /locus_tag="VFP02_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HET9542616.1"
                     /translation="MSTSPAEVDVPDVDDVVDPDRPWIVLVWNDPINLMSYVTYVFQK
                     LFGYSLEKATSLMLDVHEKGRAVVSNGTREKAELDVFRLHEHGLWATMQQEDAP"
     gene            complement(1893..>2102)
                     /locus_tag="VFP02_06030"
     CDS             complement(1893..>2102)
                     /locus_tag="VFP02_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542617.1"
                     /translation="RGWPVLAAVAAQITIGYLQYFNGVPEGLVALHVLGATLVWITVL
                     QLHLSLWTVDPAPVAPSSTAEPVPA"
BASE COUNT          300 a          707 c          796 g          299 t
ORIGIN      
        1 ccagctcgtc accctgacct gtgtcactga caccctgccg ccgatcgtca accgcgtcat
       61 cggcgacgcc tcggcccggg cgcggggctg acctaccagt gtcccgtgcc gggggcgccc
      121 ttgaaggggc cgaccaccgc ggaggtgatc cagccaccgt agaagtcgcc gtcgttgctc
      181 tcgacccgct cgtcgtcgac gaagcagccg tccatggccc gcgggtagaa ggccaggtgg
      241 tcggcgatgg cggcgaaggc cgggttcggg gccgggtagg accaggccgc gtcgcgggcg
      301 acccggtcgc ccacggtcac cgtccagtag tcggcgtggc ccttccactc acagaagctg
      361 tgcttgtccg tgcgctcgag gtgctcccgg gccacgtcct cgggcgggag gtagaaggca
      421 ggcggctggc tcgtctcgag gacccggagc gcccgggtgg tgtcgccgat caacaccccg
      481 ccgagctcga ctcggacgcg gcgatcggtg ggctcgatcc gaggcggtcg ggggtagtcc
      541 cagaccgact cctggccggg gccgggcggg atgcgggtgg ggcggagggc catgcccgac
      601 aggatggcgc cgtggaggag gacatggacc gcgccctggc cgagaaggcg gccgctctcg
      661 ccgacgccat cgacgccgcg gtgaccccgt ggctgatcgg cgccgtccgg cgggtcgccg
      721 cttcccaacg cctgcccggt ggggaccgtt tcgtcctggc cgccgagacg gcggccaacc
      781 aggccaaggc cgaggtcatg ccccggatcc gggacctgct gaccaccgac atcgacgcgc
      841 agacgaccac tccgctggcc gtcctccggg aggggacagg gccggcgaca gccgtcctgg
      901 cggagttcgg cgcccgaccg gtcgagcgag acgagttcgc ggtacgagcc ttcccggatg
      961 atgtctacgc cttggctccg gccgccttcg aggacgtcgc gccggagctg cgggacccgg
     1021 ggatcgagtg gggggcggcc aaggccttcg tccacctccg ccgccgccga gccgaaggcg
     1081 tcgtctaatc gtcctcgggg tcgggcaggg agccggtgag ggcgctgacg gtggcttcga
     1141 ggagccagcc gaggtactcg taggcggcct gggccggcgc ctcgggatcg tcggggtcga
     1201 tggggccgag gtcgtcctcg gagacgtcga ggcgggtgcc caggaccagg cgcaggtcgt
     1261 tgaaggtccg cagccaggta ccgagctgct cctcgtcgag agacgggagc acgatcgagg
     1321 actcgatctc gtcgatgttg gccagccgcc gggccaggag gtcgtcgtgg accatggcct
     1381 ggtactcggc ctcgaggtcg tcgcggccgg cgtaggcggt ggggaagagg cggcggaccc
     1441 cggactcgtc ggtgatctcg ctcccgccct ccagcttcag cagcgtccgg agctgggcca
     1501 gcagggactt cagcaggacc tgctcgggct cgctcaggcg gatggtgaag gtcccgtccc
     1561 gctcccgccg gatcggcccg cctccgaagc cgaacaccat cagggcgcgt cctcctgctg
     1621 catcgtggcc cagaggccgt gctcgtggag acggaacacg tccagctcgg ccttctcccg
     1681 ggtcccgttc gagaccacgg cccgcccctt ctcgtgcacg tccagcatca ggctggtggc
     1741 cttctccagc gagtagccga acagcttctg gaacacatag gtcacatacg acatcaggtt
     1801 gatggggtcg ttccacacga ggacgatcca ggggcggtcg gggtcgacga cgtcgtcgac
     1861 gtcggggacg tcgacctcgg ccgggctggt gctcacgccg gcaccggttc ggccgtcgag
     1921 gagggggcga ccggtgcggg gtcgaccgtc cagagagaga ggtggagctg gagcacggtg
     1981 atccacacca gggtggcgcc gaggacgtgg agggcgacca gcccctcggg cacaccgttg
     2041 aagtactgga ggtagccgat ggtgatctgg gcggcgacgg cggcgagcac gggccagccc
     2101 cg
//
LOCUS       DASTFV010000322         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1202510, whole genome shotgun sequence.
ACCESSION   DASTFV010000322 DASTFV010000000
VERSION     DASTFV010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202510"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1553
                     /locus_tag="VFP02_06035"
     CDS             <1..1553
                     /locus_tag="VFP02_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9542618.1"
                     /translation="GLRRYLAFRESRWAELVLRDRMFAHLQRLHFSFHDENQTGELMS
                     RANTDLAQVQQFVVLVPLTISNAVTVGSVAIILALIDPVLTVLALGGLPFLNWMGKRF
                     STRLHVPAMAIQRESAELASVVEESVSGIRVVKGFGAERGQSDRLRHEADDVYRESMA
                     AARVRAVYLPAMELLPNIGLVAVLGYGGHQVLDGSLSLGSFVAFNVYVVLLIWPLRML
                     GMIIAQAQRSAASAQRVHEILVTDPDIADRKDAVSLPPRDDSHAGGEVCFEDVDFGYG
                     AGGMPVLSGFVLRVAPGESVALVGATGSGKSTVAHLLPRFYDVDGGRVRLDGVDVRDV
                     KLVDLRRAIGIVFEETFLFHDTVAANIAFADGAADQEQIERAARLAGAHEFISDLPDG
                     YGTIVGERGFSLSGGQRQRLAIARAILADPRVLILDDATSAVDPTKEHEIRGALGEVM
                     AGRSTIVIAHRPATVALADRVILVDEGQVAADGTHEELLATNARYREVLASSDGPGAD
                     GKAVADVG"
     gene            1543..>2328
                     /locus_tag="VFP02_06040"
     CDS             1543..>2328
                     /locus_tag="VFP02_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9542619.1"
                     /translation="MWGEAGVAEEDMLDAEAAKQVLRRAARMLAPYRRKVVVATLLLF
                     AWTGCVLAGPFLVRFAIDDGLTADDVGALNLAVGLYVAVALIGYVVYRRVVLLVSVVG
                     ESFLRDIRIRVFAHLMRLSMPFYDREKAGVVVSRMTSDVESLQELVQFGLLMFVTNTL
                     LLVLAFIALAVVSWQLLLVCLVALPPVILASIRFQRQSNQAYLVVRDRIGSTLSDLQQ
                     GITGVRVIQAFGREDAEVGRFRSGNRTLYDAHMDSVRIQAWYLP"
BASE COUNT          292 a          837 c          808 g          391 t
ORIGIN      
        1 cggggctgcg ccgctacctg gcgttccggg agagccggtg ggccgagctg gtcttgcggg
       61 accggatgtt cgcccacctc cagcgcctcc acttctcctt ccacgacgag aaccagaccg
      121 gcgagctgat gagccgggcc aacacggacc tggcccaggt ccagcagttc gtggtcctgg
      181 tcccgctgac gatctccaac gcggtcacgg tgggctcggt cgcgatcatc ctcgccctca
      241 tcgaccccgt gctcaccgtc ctcgccctgg gcggcctgcc gttcctcaac tggatgggca
      301 agcgcttctc gacccggctg cacgtgcccg ccatggccat ccagcgggag tcggccgagc
      361 tggcctcggt ggtcgaggag tcggtctcgg gcatccgcgt ggtgaagggc ttcggcgccg
      421 aacggggcca gtccgaccgg ctccggcacg aggccgacga cgtctaccgg gagtccatgg
      481 ccgcggcccg ggtccgtgcc gtctacctgc ccgccatgga gttgctgccc aacatcggcc
      541 tcgtcgccgt cctcgggtac ggcggccacc aggtcctcga cggcagcctc agcctcgggt
      601 ccttcgtggc cttcaacgtc tacgtcgtgt tgctgatctg gcctttgcgg atgctgggca
      661 tgatcatcgc ccaggcccaa cggtcggcgg cctccgccca gcgggtccac gagatcctgg
      721 tcaccgatcc cgacatcgcc gatcgcaagg acgcggtcag cctccccccg cgggacgaca
      781 gccacgccgg gggtgaggtc tgcttcgagg acgtcgactt cggctacggc gccgggggga
      841 tgcccgtgct gtcgggcttc gtgctgcggg tcgctccggg tgagtccgtg gccctcgtcg
      901 gagccaccgg ctcgggcaag tcgaccgtgg cccacctcct gccccgcttc tacgacgtcg
      961 acggcggccg ggtccgcctg gacggcgtcg acgtgcggga cgtgaagctg gtcgacctgc
     1021 gccgggccat cggaatcgtg ttcgaggaga ccttcctctt ccacgacacc gtggccgcca
     1081 acatcgcctt cgccgacggc gccgccgacc aggaacagat cgagcgggcg gctcgcctgg
     1141 ccggcgccca cgagttcatc agcgacctgc cggacggcta cgggacgatc gtgggggagc
     1201 ggggcttctc tctgtccggg ggccagcgcc agcgtcttgc catcgcccgg gcgatcctgg
     1261 ccgatccccg cgtcctcatc ctcgacgacg ccacgtcggc ggtggacccg accaaggagc
     1321 acgagatccg gggtgccctg ggcgaggtca tggcggggcg gagcacgatc gtcatcgccc
     1381 accggccggc gaccgtggcc ctggccgacc gggtcatcct cgtcgacgag ggccaggtgg
     1441 ccgccgacgg cacgcacgag gagctcctgg ccaccaatgc ccgttaccgg gaggtgctgg
     1501 cgtcgtccga cggcccgggc gcggacggga aggcggtggc cgatgtgggg tgaggccggc
     1561 gtcgccgagg aggacatgct cgacgccgag gcggccaagc aggtcctgcg gcgggcggcc
     1621 cggatgttgg cgccgtaccg gcgcaaggtc gtcgtggcca cgctcctgct gttcgcctgg
     1681 accggatgcg tgctcgccgg gccgttcctc gtccggttcg ccatcgacga cgggctgacc
     1741 gccgacgatg taggcgccct gaacctggcc gtcggcctct acgtggccgt cgccctgatc
     1801 ggatacgtcg tctatcggcg ggtcgtcctg ctggtcagcg tggtggggga gtccttcctc
     1861 cgcgacatcc gcatccgggt gttcgcccac ctcatgcgcc tgtcgatgcc cttctacgac
     1921 cgggagaagg ccggcgtcgt ggtctcgcgg atgacctccg acgtcgagtc gctgcaggag
     1981 ctggtccagt tcggcctgct catgttcgtg accaacaccc tgctgctggt cctcgcgttc
     2041 atcgccctgg ccgtcgtctc ctggcagctc ctgctcgtct gcctggtggc tctgccgccg
     2101 gtgatcctgg ccagcatccg gttccagcgg cagtccaacc aggcctacct cgtggtccgg
     2161 gaccggatcg gctcgacgtt gtcggacctc cagcagggca tcaccggtgt gcgggtcatc
     2221 caggccttcg ggcgggagga cgccgaggtc gggcgcttcc ggtccggcaa ccggaccctc
     2281 tacgacgccc acatggactc ggtgcggatc caggcctggt acctgccc
//
LOCUS       DASTFV010000323         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1203314, whole genome shotgun sequence.
ACCESSION   DASTFV010000323 DASTFV010000000
VERSION     DASTFV010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203314"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1106)
                     /locus_tag="VFP02_06045"
     CDS             complement(111..1106)
                     /locus_tag="VFP02_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HET9542620.1"
                     /translation="MADPSSRMLRLLSLLQTHRHWAGAELANRLEVSERTLRRDVDRL
                     RELGYPVDATRGTDGGYQLAAGSQLPPLLLDDEEAVAIAVGLRAAAVSPVAGIDETSV
                     RALAKLEQVLPARLRHRVGALQHYSEPLTRGVARGREEPTINPSDLTEIAGACRDTVR
                     LRFDYERRDGVAGGRMVEPHRLVSTGRRWYLVAWDVDREDWRTFRVDRMQEPKALTRT
                     FAARELPEGVDAAAFVEAGLQSAWAPAEGVVTVAVPLDEATGYSPMCGMSVEVVDATS
                     CRLRLRADSWEWLAVVVALLPFPIVSVEPADLRDHVQAMVQRLTPAPSGSIGDHH"
     gene            1169..2131
                     /locus_tag="VFP02_06050"
     CDS             1169..2131
                     /locus_tag="VFP02_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9542621.1"
                     /translation="MMIQTQGLTRHFGTKEGTVEAVRDLDLEVQEGELVALLGPNGAG
                     KSTTMRMLTTLLTPTAGEARVVGHDVVAEPGEVRRGIGYVGQGNGAGQNQRVRDELVT
                     QGRAYGLATDESRRRSDDLLDALDLVALADRAANTLSGGQRRRLDVALGLVHRPQLLF
                     LDEPSTGLDPQSRANLWEHILRLRREHGMTIVLTTHYLDEADSMAERVMVIDHGVVIA
                     DDTADRLKAQLAGDRIVLVTDGDVSARRVVELAGRVAQAQEVEVDAGTVSLRVVEGAG
                     TLPGLLRALDADGLDVQAAEVRRPTLDDVFLTLTGRSLREDVAA"
     gene            2128..>2427
                     /locus_tag="VFP02_06055"
     CDS             2128..>2427
                     /locus_tag="VFP02_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542622.1"
                     /translation="MSIITPDRPTDRRPARPGAEPIPARSQNVPTLVTDTMTVMTREL
                     RPTLRNPFSVIFTMVQPLFFLALFGPLLISVTGAGTAATLQTFVPGILVMSALFAA"
BASE COUNT          341 a          877 c          882 g          327 t
ORIGIN      
        1 acagtcggta gatgccgtcg gccacttcgt tgacgttcgt ctccatcagg caagcacctc
       61 gtggtcagga ggccggcgcg ggtggaccgg ccggagcatt ttggcagagc tcagtggtgg
      121 tcaccgatcg atcccgacgg ggcgggggtg aggcgctgga ccatggcctg gacgtggtcg
      181 cggaggtcgg cgggctcgac gctgacgatc ggaaagggga ggagggcgac gacgacggcc
      241 agccactccc agctgtcggc tcggaggcgg agtcggcagc tcgtggcgtc gacgacctcg
      301 acggacatgc cgcacatggg ggagtacccc gtcgcctcgt cgaggggcac ggcgacggtg
      361 accacgccct cggcgggggc ccaggccgac tgcagccccg cctcgacgaa ggcggcggcg
      421 tccacgccct ccggcaactc tcgggcggcg aaggtccggg tcagggcctt gggctcctgc
      481 atccggtcga cccggaaggt ccgccagtcc tcccggtcga cgtcccaggc cacgaggtac
      541 cagcgccgcc cggtcgacac caggcgatgg ggttcgacca tgcgtccacc ggcgacgccg
      601 tcgcgccgtt cgtagtcgaa gcggaggcga accgtgtccc ggcaggcccc ggcgatctcg
      661 gtcaggtccg aggggttgat ggtcggctcc tcccggcccc gggccacgcc gcgggtcagc
      721 ggttcggagt agtgctggag ggcgcccacc cggtggcgga gccgggcggg caggacctgc
      781 tcgagcttgg ccaaggcccg taccgaggtc tcgtcgatgc cggccaccgg gctgaccgcg
      841 gccgcccgga ggccgacggc gatggcgacc gcctcctcgt catcgaggag gagcgggggg
      901 agctggctcc cggcggccag ctggtagccg ccgtcggtgc cccgggtggc atcgacgggg
      961 tagccgagct cgcggaggcg gtcgacgtcg cggcgcagcg tgcgctcgga cacctccagc
     1021 ctgttggcca gctcggcccc cgcccagtgg cggtgggtct gcaggaggga gaggagccgg
     1081 agcatgcggg agctgggatc ggccatgccc ggaagattct tccaccttcc ggccagaatc
     1141 tgtccggaag aggtgcgatt ctcgggtcat gatgatccag acccagggcc tgacccggca
     1201 cttcggcacg aaggagggga cggtcgaggc cgtccgcgac ctggacctcg aggtccagga
     1261 gggcgagctc gtcgccctcc tcggtcccaa cggcgccggc aagtcgacga cgatgcggat
     1321 gctgaccacc ctgctgacgc cgaccgcggg cgaggcccgg gtcgtgggcc acgacgtggt
     1381 ggccgagccg ggcgaggtcc ggcgcggcat cggctacgtc ggccagggca acggcgccgg
     1441 ccagaaccag cgggtgcgcg acgagctggt cacccagggt cgggcctacg ggctggccac
     1501 agacgagtcc cgccgccgca gcgacgacct gctcgatgcc ctcgacctcg tcgccctcgc
     1561 cgatcgggcc gccaacacgc tctccggcgg ccagcgccgg cgcctggacg tggccctcgg
     1621 cctggtgcac cgtccccagc tgctcttcct ggacgagccg tcgaccggcc tcgaccccca
     1681 gagccgggcc aacctctggg agcacatcct gcgcctccgc cgggagcacg gcatgacgat
     1741 cgtgctcacc acccactacc tggacgaggc ggactccatg gccgagcggg tcatggtcat
     1801 cgaccacggc gtggtcatcg ccgacgacac cgccgaccgc ctgaaggctc agctggccgg
     1861 cgatcgcatc gtcctcgtga ccgacggcga cgtgtccgct cgccgcgtcg tggagctcgc
     1921 cggccgcgtc gcccaggccc aggaggtcga agtcgacgcc gggacggtga gcctgcgggt
     1981 cgtggagggg gcaggcaccc tgcccggcct gctccgggcc ctcgacgccg acggcctgga
     2041 cgtacaggcc gccgaggtcc gtcgccccac cctcgacgac gtcttcctga ccctcacggg
     2101 ccggagcctg cgagaggacg tggcggcatg agcatcatca cccccgaccg acccaccgat
     2161 cgccggccgg cccgtcccgg ggccgagccc attccggcaa ggagccagaa cgtgcccacc
     2221 ctcgtgaccg acacgatgac cgtgatgacc cgggagctgc ggccgacgct gcggaacccg
     2281 ttctcggtca tcttcacgat ggtccagccg ctcttcttcc tggccctctt cggccccctg
     2341 ctgatctccg tcacgggcgc ggggacggcc gccacgctgc agaccttcgt gcccgggatc
     2401 ctggtcatgt ccgcgctgtt cgccgca
//
LOCUS       DASTFV010000324         3790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1207231, whole genome shotgun sequence.
ACCESSION   DASTFV010000324 DASTFV010000000
VERSION     DASTFV010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207231"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VFP02_06060"
     CDS             complement(<1..566)
                     /locus_tag="VFP02_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542623.1"
                     /translation="MKIAVYNRYWRTGGGAETYGAAVAQVLARHPEHTVDLLGPDDVD
                     LTRLAERLRLDLAGIGMVVLRDRPGAVLRASKGYDLFINVSFMSDDDAGAPHNLYITH
                     FPARSARDLSWAQRGAIRVLGPLVRAQAVDTAWGKGFYPREGRRPRYFWTADEASFFV
                     EARADRPVPVRMLFRRQRPADLPPAHVEV"
     gene            complement(563..1951)
                     /locus_tag="VFP02_06065"
     CDS             complement(563..1951)
                     /locus_tag="VFP02_06065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9542624.1"
                     /translation="MSPRVRAVVLNFNGGRHVIDCVEALRRTDWPAADFEVVVVDNAS
                     SDGSDGELRRRFPDLEIRPTGANLGFPANNVAMGDLDGFDHVALVNNDAFVDPGWLRP
                     LVDALDADPGLGAACPRILFAPRFLDLTITSPTFRPGTGDGRDLGVRLSGVEVDGEDV
                     WRDAQRVQGFWGIEHGAGDEAQFEWTQGRAVVRVPVPGVGDDDAVQVRLRVAAESAKA
                     VTVAAGSEPVALDVGTDASWVEVTLTGEPYDVVNNVGSVLIEGGYGADRGYLQRDDGQ
                     FDGPDEVFAWCGGGVLLRRRYLEEVGLFDERFFLYYEDTDLAWRGRSRGWRYRYVPES
                     RIRHIHAASSGEGSALFQHFVERNRLLLLTKNAPRALAASAAARFVLITASYARRDVV
                     APVLRGRRPNLVLFRRRTRSFLGYLALLPAMLVARFRNRRRQTVDDADLIGWAIPQPE
                     PLARLAGEDEER"
     gene            2016..3122
                     /locus_tag="VFP02_06070"
     CDS             2016..3122
                     /locus_tag="VFP02_06070"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HET9542625.1"
                     /translation="MKGLILSGGAGTRLRPITHTSAKQLVPVANKPILFYGLEDMAEA
                     GIREVGIVIGETGDEIRGAVGDGSAWGLEVTYLPQDEPLGLAHCVLIAADFLGREDFV
                     MYLGDNLLRQGIAEFVDSFEADRAASAAPSLDGAANPPSAQILLARVPDPQRFGVAEI
                     GPDGEVLQLLEKPDEPPSDLALVGVYLFDATIHEAVAAIEPSPRGELEITDAIQWLID
                     HGHRVRHEILDGWWKDTGKLDPLLEGNRLILETLERAVRGSVDGASSVEGRVVVEDGA
                     EILSSRVRGPAIIGTGTRIVNSYVGPYTSVAADCEIIDSEIEHSVILENSRIVNIPHV
                     QDSLIGREVEVVRSGRVPRATRLMLGDHSRVDIE"
     gene            3137..3703
                     /gene="rfbC"
                     /locus_tag="VFP02_06075"
     CDS             3137..3703
                     /gene="rfbC"
                     /locus_tag="VFP02_06075"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HET9542626.1"
                     /translation="MAEVTESPTIRDVYVVTPKVFGDERGVFVETYRREWFPQGREMV
                     QANRSDRRAGSVVGLHYHLHQADYWYVPTGRARVVLHDLREGSATDGNTLVLDLGEDA
                     DGTHPHRGVFIPPGVAHGFAALTDMTMTYLVDGYYNPADELGVAWDDPAIAADWGVTD
                     PVLSDRDRTNPRRADLDQGRRPHIALRT"
BASE COUNT          581 a         1357 c         1345 g          507 t
ORIGIN      
        1 acctccacgt gggccggcgg caggtcggcg gggcgctgcc ggcggaacaa catgcgtacg
       61 ggcaccggac ggtcggcccg ggcctcgacg aagaacgacg cctcgtccgc ggtccagaag
      121 tagcggggcc gccgcccctc ccgtgggtag aagcccttgc cccatgccgt gtccaccgcc
      181 tgggcccgga ccaggggccc gaggacccgg atggcgcccc gctgggccca cgacaggtca
      241 cgggccgagc gggccgggaa gtgggtgatg tagaggttgt gaggggcgcc ggcgtcgtcg
      301 tcgctcatga acgacacgtt gatgaacagg tcgtagccct tgctggcccg cagcacagcg
      361 ccgggccggt cccgcaggac gaccatgccg atgcccgcga ggtcgaggcg caggcgttcg
      421 gccaaccggg tcaggtccac gtcgtcgggg cccaacaggt cgacggtgtg ctcggggtgc
      481 cgcgccagga cctgggccac cgccgccccg taggtctcgg ccccgccacc ggtgcgccag
      541 taccggttgt agacggcgat cttcatcgct cctcgtcctc accggcgagc cgcgccagcg
      601 gctcgggctg gggaatcgcc caaccgatca ggtccgcatc gtcaacggtc tgtcgccgcc
      661 ggttccggaa ccgggccacc agcatcgccg ggagcagagc cagatagccg aggaaggaac
      721 gggtgcgccg ccggaacagc acgaggttgg gacgacggcc ccgcaggacc ggggccacga
      781 cgtcgcgccg ggcgtagctc gcggtgatga gcacgaagcg ggcggcggcg gaagcggcga
      841 gcgcccgggg cgcgttcttc gtcagcagca gcaggcggtt gcgctcgacg aagtgctgga
      901 agagggccga gccttcgccg ctgctggcgg cgtggatgtg ccggatccgg ctctcgggca
      961 cgtagcggta ccgccagccg cggctccggc cccgccaggc caggtcggtg tcctcgtagt
     1021 agaggaagaa gcgctcgtcg aacaggccca cctcttcgag gtagcgccgg cgcaggagga
     1081 ccccgccacc acaccacgca aacacctcgt cgggcccgtc gaactgaccg tcgtcgcgct
     1141 ggaggtagcc gcggtccgcg ccgtaccccc cttcgatcag gaccgagccg acgttgttga
     1201 ccacgtcgta gggctccccc gtcagcgtca cctccaccca ggacgcgtcc gtccccacgt
     1261 cgagtgccac cggctcggac ccggcggcga cggtcacggc cttggcggac tccgcggcga
     1321 cgcggagccg cacctggacc gcgtcgtcgt ccccgacccc tggcaccggc acccggacca
     1381 ccgcccgtcc ctgggtccac tcgaactggg cctcgtcgcc ggcgccgtgc tcgatgcccc
     1441 agaacccctg cacccgctga gcgtcccgcc agacgtcctc cccgtcgacc tcgacccccg
     1501 aaaggcggac ccccaggtct cgcccgtcac cggtgccggg ccggaaggtg gggctggtga
     1561 tggtcaggtc caggaaccgg ggggcgaaga ggatccgggg acaagccgcg ccgagcccgg
     1621 gatcggcgtc cagagcatcg acgagcggtc gcagccagcc ggggtccacg aaggcgtcgt
     1681 tgttgacgag ggcgacgtgg tcgaacccgt cgaggtcgcc catggccacg ttgttggccg
     1741 ggaagccgag gttggcgccg gtcggccgga tctccaggtc cgggaagcgg cgccggagct
     1801 ccccgtcgct gccgtcggag gaggcgttgt cgacgaccac gacctcgaag tcggccgccg
     1861 gccagtcggt gcggcgcagc gcctccacgc agtcgatgac gtgacggccg ccgttgaagt
     1921 tcaggacgac ggcacggacg cggggggaca cggggccgcg aggatagagc gctcaccctg
     1981 aggggcgcga ggtggggcgt ggtacaacga cggccgtgaa gggcctcatc ctttccggcg
     2041 gggccggcac gcgccttcgg ccgatcaccc acaccagcgc caaacagctg gtgccggtgg
     2101 ccaacaagcc catcctgttc tacgggttgg aggacatggc cgaggccggc atccgggagg
     2161 tcggcatcgt catcggcgag accggtgacg agatccgcgg ggcggtgggc gacggctccg
     2221 cctggggcct ggaggtcacc tacctccccc aggacgagcc gttggggctg gcccactgcg
     2281 tgctcatcgc cgccgacttc ctgggccggg aggacttcgt catgtacctc ggcgacaacc
     2341 tgctgcgcca gggcatcgcc gagttcgtcg acagcttcga ggccgaccgg gccgcgtcgg
     2401 ccgcaccgtc gctcgacggc gccgcgaacc cgccgtcggc ccagatcctg ctggcccgtg
     2461 tgcccgaccc gcaacggttc ggcgtggccg agatcggtcc cgacggcgag gtcctgcaac
     2521 tactggagaa gcccgacgag cccccgtccg acctcgctct ggtcggcgtc tacctgttcg
     2581 acgccaccat tcacgaggcc gtggccgcca tcgagccgtc gccccggggc gagctggaga
     2641 tcaccgatgc cattcaatgg ctcatcgacc acggccatcg ggtccgccac gagatcctgg
     2701 acggctggtg gaaggacacg ggcaagctcg acccgctcct ggagggcaac cgcctgatcc
     2761 tggagacgct cgagcgggcc gtgcggggct cggtggacgg ggcgtccagc gtcgagggcc
     2821 gcgtcgtggt cgaggacggg gccgagatcc tgtccagtcg ggtccgaggc ccggccatca
     2881 tcgggacggg cacgcgtatc gtgaacagct acgtgggccc gtacacctcc gtcgctgccg
     2941 actgcgagat catcgactcc gagatcgagc actcggtgat cctggagaac agccgcatcg
     3001 tcaacatccc ccacgtacag gactcgctga tcggcaggga ggtcgaggtc gtgcgatcgg
     3061 gccgggtccc ccgggcgacc cgactgatgc tgggcgacca ctcccgcgtc gacatcgaat
     3121 aggaccggtg agcgccatgg ccgaagtgac cgaatccccc accatccgcg acgtgtacgt
     3181 cgtgaccccc aaggtgttcg gcgacgagcg gggcgtcttc gtcgagacct accggcgcga
     3241 gtggttcccg cagggacggg agatggtcca ggccaatcgc agtgatcgcc gagccggctc
     3301 ggtggtcggc ctgcactacc acctgcacca ggccgactac tggtacgtgc cgacgggccg
     3361 ggcccgggtg gtcctccacg acctgcggga gggctccgcc accgatggga acaccctcgt
     3421 gctcgacctc ggcgaggacg ccgacggcac ccatccccac cggggcgtgt tcatcccgcc
     3481 cggcgtggcc cacggcttcg ccgccctcac cgacatgacg atgacctacc tggtcgacgg
     3541 ctactacaac ccggcggacg aactgggcgt ggcctgggac gacccggcta ttgccgcgga
     3601 ctggggggtg accgacccgg tcctgtccga ccgagaccgc acgaaccccc ggcgcgccga
     3661 cctcgaccag ggtcgccgcc cccacatcgc cctgcggacc tgaggaggca gtcctgtgag
     3721 caagctgttc gtcaccggtg gggccggctt catcggctcg aactacgtgg cctggatcct
     3781 ggccaactcc
//
LOCUS       DASTFV010000325         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1210694, whole genome shotgun sequence.
ACCESSION   DASTFV010000325 DASTFV010000000
VERSION     DASTFV010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210694"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VFP02_06080"
     CDS             <1..750
                     /locus_tag="VFP02_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET9542627.1"
                     /translation="IARETERIRLGTLVTSATFRYPGPLAVAVAQVDEMSGGRVELGI
                     GAGWFDGEHDAHGIPFPATGERFDRLEEQLAVITGLWETPVGETFSFDGQHYPVKNSP
                     ALPKPVQSPRPPVIIGGHGPKRTPRLAATYADEFNFGFSPIADFTAQVERVTAACEAR
                     DRDPATMTWSVALTAVMGADEADFQRRAEAIGQDPEGLRSGQLGGTPDQIRDRLGQWA
                     DAGAQRVYLQVLDLRDLDHIALLGEELVGQI"
     gene            complement(827..2245)
                     /locus_tag="VFP02_06085"
     CDS             complement(827..2245)
                     /locus_tag="VFP02_06085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9542628.1"
                     /translation="MTPAAEAEVPRRAWVVLLLACMAGFAGALDLSVVFVAFPAIEDA
                     FPDASTALLSWVLTIYGIVIAALLVPGGRLADLFGRRKVFLIGVVIFGLGALASGAAP
                     DPGVLIATRAFQACGGALMTPAAMALVLREFPADRRAMAIGTWGAVGGVAAACGPPLG
                     ALVVDLGGWRWAFWLSVPLALVVFVWGRVVLVESVDPDVHGLTDLFGSFLVMVAVGSL
                     ALAIVEGPSWGWTSAGVLGALALSAVSAVVLVRRSTRHPDPVIAPDLFAAPLFRRANV
                     VALLFGAAFFAMFFGLVRFLTEGWGYSTGQAGLLVTPGPAAAALFSYIGGNLADRFGH
                     RAVMVPGALIFAIGGAWLALGIGEEPAIWTVWVPAVLLLGVGIGLVFPNFQSAAVHGV
                     PEARFGVAAAAIQTVVRLSGTIGIALAVVLVGSVVAADPVADFDPLWWTLVLLGLLSA
                     AVSLGIDTRSPVSHRVTTDAPA"
     gene            complement(2242..>2462)
                     /locus_tag="VFP02_06090"
     CDS             complement(2242..>2462)
                     /locus_tag="VFP02_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542629.1"
                     /translation="HRRQVRTLRRQEILFGLATLAGRYRDALADGRDPAELVDGLAAL
                     QTAAEDLVRNPAEELLLLGLFLKLPHLR"
BASE COUNT          415 a          896 c          903 g          248 t
ORIGIN      
        1 atcgcccgcg agaccgagcg catccggctg ggcacgctgg tcacctcggc caccttccgc
       61 tacccgggac cgctggccgt cgcggtggcc caggtcgacg agatgagtgg gggccgggtc
      121 gagctgggca tcggcgccgg ctggttcgac ggggagcacg acgcccacgg catcccgttc
      181 cccgccacgg gcgagcgctt cgaccggctc gaggagcagc tggcggtcat caccggcctg
      241 tgggagaccc cggtcggcga gaccttctcg ttcgacggac agcactaccc cgtcaagaac
      301 tcccccgccc tgcccaagcc cgtgcagtcg ccgcggccgc cggtcatcat cggcggccac
      361 ggtccgaagc ggacaccgcg gctggccgcc acctatgccg acgagttcaa cttcggcttc
      421 tccccgatcg ccgacttcac cgcccaggtg gagcgggtga ccgcggcctg cgaggcccgg
      481 gaccgggacc cggccacgat gacctggtcc gtcgccctga ccgcggtgat gggcgccgac
      541 gaggccgatt ttcagcgacg ggctgaggcc atcggccagg accccgaagg gttgcgctcg
      601 ggccagctcg gcggcacgcc cgaccagatc cgggaccgcc tcggccagtg ggccgacgcc
      661 ggcgcccagc gcgtctacct ccaggtcctc gacctccgcg acctcgacca catcgccctc
      721 ctcggcgaag agctcgtcgg ccagatctga ttccaccgcg aatccgagcc tgcgccgaga
      781 gcaccacgat tgtgtggttt cttcgacgca agttcgggta cgagccctag gcgggcgcgt
      841 cggtcgtcac gcggtgggac acgggcgaac gggtgtcgat gccgaggctg acggcggcgg
      901 acaggaggcc gagcaggacg agggtccacc acaacgggtc gaagtcggcc acggggtcgg
      961 cggcgacgac actgcccacc agcacgacgg ccagtgcgat gccgatggtg cccgagaggc
     1021 ggaccacggt ctggatggcg gccgcggcca caccgaagcg ggcttcgggg acgccgtgca
     1081 ccgcggcact ctggaagttc gggaacacca ggccgatgcc gacccccagc agcaggaccg
     1141 cggggaccca caccgtccag atggccggct cttcgccgat gccgagcgcc agccacgccc
     1201 caccgatggc gaagatcagc gcgcccggca ccatgacggc ccggtggccg aaccgatcgg
     1261 ccaggttgcc gccgatgtag gagaacagcg cggccgccgc cggtccgggg gtgaccagca
     1321 gcccggcctg cccggtcgag tacccccacc cctcggtcag gaagcggacc aggccgaaga
     1381 acatggcgaa gaaggcggcg ccgaacaaca gcgcgaccac gttggcccgg cggaacaggg
     1441 gcgcggcgaa caggtcgggg gcgatgaccg ggtccgggtg ccgggtggac cgtcggacca
     1501 gcaccacggc ggagacggcc gacagggcca gggccccgag cactccggcg gaggtccagc
     1561 cccacgacgg gccctcgacg atggccagtg ccagcgagcc gaccgccacc atgaccagga
     1621 acgagccgaa caggtcggtc aggccgtgga cgtccgggtc gaccgactcg accaggacga
     1681 cccggcccca cacgaagacg acgagggcca ggggcacgct cagccagaac gcccagcgcc
     1741 agccgccgag gtccaccacg agggcgccga gcggcggacc gcaggcggcg gccactccgc
     1801 cgacggcccc ccaggtgccg atggccatgg cccgacggtc agcggggaac tctcgcagca
     1861 ccagggccat cgccgccggc gtcatcagag ccccgccgca cgcctggaag gcccgggtgg
     1921 cgatgaggac gccggggtcg ggggcggcgc cactggccag cgcccccagc ccgaagatca
     1981 ccacgccgat caggaagacc ttgcgccgac cgaacaggtc cgccaggcgg ccgccgggga
     2041 ccagcagggc ggcgatgacg atgccgtaga tggtcaacac ccaggagagc agcgcggtcg
     2101 aagcgtcggg gaaggcgtcc tcgatggccg ggaaggccac gaacaccacg gacaggtcga
     2161 gcgccccggc gaagccggcc atgcaggcca gcaggaggac gacccacgcc cggcggggga
     2221 cctccgcctc cgccgccggc gtcaccgcag gtgaggcagc ttcaggaaca ggccgaggag
     2281 gaggagctcc tcggccgggt tgcggacgag gtcctcggct gccgtctgga gggcggcgag
     2341 gccgtcgacg agctcggccg ggtcacggcc gtcggccaag gcgtcgcggt agcggccggc
     2401 gagggtggcc agcccgaaga ggatctcctg acggcgcagg gtccggacct ggcgccggtg
     2461 cc
//
LOCUS       DASTFV010000326         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1211846, whole genome shotgun sequence.
ACCESSION   DASTFV010000326 DASTFV010000000
VERSION     DASTFV010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211846"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1339
                     /locus_tag="VFP02_06095"
     CDS             80..1339
                     /locus_tag="VFP02_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET9542630.1"
                     /translation="MGDEGKDWFHGAGLGLFVHWTHSSAAGRELSWPLVGGGPVLPHS
                     GEATVDEYYASVADFSPSPGAAREWCTAAAAAGARYAVLTTRHHDGYALWPSAHTSLG
                     VRDSGIDRDLVAEFVDACRSEGLRVGFYLSLSDWHHPDYPAFRDEDRPYPFIAYPRPE
                     PEAWARYVDDLFGQVRELLTDYGEIAVLWFDGGWERGATEWRAAELESLIRELQPDIL
                     LNERLPSVADYTTPEQFVPPTAPEGRWETCLTMNESWGYVPDDEDWKSARDLVHALCE
                     TVGKGGNLLLNVGPRADGSLDPTEASRLSAVADWMDRYGSAVHDVEAGLEPWQWYGPS
                     TRQGDRVHLMCVQRPYDSVTVRGVPTKRVERVIDLATGKELEWNTRCSVLDELFNSDP
                     HGEVRIEVPEAVVDDLATVLALDLSPA"
     gene            complement(1356..2258)
                     /locus_tag="VFP02_06100"
     CDS             complement(1356..2258)
                     /locus_tag="VFP02_06100"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET9542631.1"
                     /translation="MAVPRMHEDEVEVDDVLVRRLLDDQLPQFADRPLRRIETWGTDH
                     VIFRLGGDLSVRLPKIGWAATQGEKEHRWLPVLAPQLPVEVPVPLAVGGPAAGYPFAW
                     YVSPWLAGTNPDGAVDRRGLAVDMAAFVLALQQVDTAGAPTPEQGQRGGPLAGADQFT
                     RARADELRDEADVDALLAVWDAGLDAPPWEGAGVWAHGDLMDGNLLVRDGRLSGVIDW
                     GGLRAADPAVELMVAWSFFDAEERTVYRDALGFVDDAMWLRGRAWATSAALQALPYYR
                     DTNPDIVARSWRAVREVLADLDHT"
     gene            complement(2336..3070)
                     /locus_tag="VFP02_06105"
     CDS             complement(2336..3070)
                     /locus_tag="VFP02_06105"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HET9542632.1"
                     /translation="MTGHTIETAERWRAARLELLEAEKALTRRSDEVARQRQALPWVR
                     VEKDYRFEGEDGEVTLRDLFRGRSQLLVHHFMFPDPAQGCPSCSSVADGYDGFRVHLE
                     GHDVAMATVSRAPIDAITAYRTRMGWSFPWVSSMGSDFNFDFGVSYTEAQLAAGAEHN
                     YRPLAIDVAALPHDGETDEPFPEAESPGLSTFVLDDGEVFHAYSAYARGTDGLWGMWQ
                     WLDRAPLGRNEGDFSWFHRHDEYEDA"
BASE COUNT          483 a         1181 c         1154 g          475 t
ORIGIN      
        1 ccccctcgga aaactggaac gcgttctagt caccctacgc gtaatccgga ggtcgatttc
       61 gcggctacgg tcgccggtgg tgggggacga agggaaggac tggttccacg gcgccgggtt
      121 gggcctcttc gtgcactgga cccactcgtc cgccgccggc cgggagctgt catggcccct
      181 ggtcggcggc ggaccggttc tgccccacag cggtgaagcc accgtcgacg agtactacgc
      241 gtcggtggcc gatttctcgc cctctccggg ggcggctcgg gagtggtgca cggcggccgc
      301 tgcggcggga gcccgttacg ccgtgctcac cactcgccac cacgacggct atgccctctg
      361 gcccagcgcc cacaccagcc tcggcgtgcg cgactcgggg atcgaccggg acctggttgc
      421 cgagttcgtg gacgcctgcc ggagcgaggg cctccgggtc ggcttctacc tctcgctgtc
      481 ggactggcac catcccgact acccggcctt ccgcgacgag gaccggccct acccgttcat
      541 cgcctacccc cggcccgagc ctgaggcgtg ggcccggtac gtcgacgacc tgttcggcca
      601 ggtccgggag ctgctgaccg actacggcga gatcgccgtg ctctggttcg acggcgggtg
      661 ggaacggggg gcgacggaat ggagggccgc cgagctggag tccctgatcc gggagctcca
      721 gcccgacatc ctcctcaacg aacgcctgcc ctccgtcgcg gactacacga cgccggagca
      781 gttcgtcccg ccgacggcgc cggagggccg gtgggagacc tgcctgacca tgaacgagag
      841 ctggggctac gtccccgacg acgaggactg gaagtcggcg cgggacctgg tccatgcgct
      901 gtgcgagacc gtgggcaagg gcgggaacct gctgctgaac gtcggccccc gggcagacgg
      961 atccctcgac ccgaccgagg cgtcgcggct gtcggctgtg gccgactgga tggatcggta
     1021 cggctcggcc gtccacgacg tcgaggccgg cctggagccc tggcagtggt acggcccgtc
     1081 gacccggcag ggcgaccggg tgcacctgat gtgcgtccag cgcccctacg actcggtcac
     1141 ggtgcggggc gtcccgacga agcgggtcga gcgggtgatc gacctggcga cgggcaagga
     1201 gctcgagtgg aacacccgct gctcggtcct ggacgagctc ttcaacagtg acccccacgg
     1261 cgaggtccgc atcgaggtcc ccgaggccgt cgtcgacgac ctggcgaccg tcctcgccct
     1321 cgacctgagc cccgcctgag ccctcagccc ggcgatcagg tgtggtcgag atcggccagg
     1381 acttcgcgga cggcccgcca ggaccgggcc acgatgtcgg ggttggtgtc gcggtagtac
     1441 gggagggcct ggagggccgc cgaggtcgcc caggcccgac cccgcagcca catggcgtcg
     1501 tccacgaagc cgagcgcatc ccggtagacg gtgcgctcct cggcgtcgaa gaagctccac
     1561 gccaccatca gctcgaccgc cgggtcggcc gcccgcagcc ctccccagtc gatgaccccg
     1621 ctgaggcgac cgtcgcggac gagcaggttg ccgtccatga ggtccccgtg ggcccacacg
     1681 cccgcaccct cccagggcgg ggcgtcgagg cccgcgtccc agacggcgag cagcgcgtcc
     1741 acatccgctt cgtcccgtag ctcgtcggcc cgggcccgcg tgaactggtc agcgccggcg
     1801 agcggtccac cccgctggcc ttgctccggc gtcggcgcgc ccgccgtgtc cacctgctgg
     1861 agggccagca cgaatgcggc catgtcgacc gccagaccgc gtcgatcgac cgcgccgtcg
     1921 gggttcgtcc cggctagcca cggcgagacg taccaggcga acggatagcc ggcggcgggc
     1981 ccgcccactg cgagcggcac cggcacctcg accgggagct gcggcgcgag cacggggagc
     2041 caccggtgct ccttctcgcc ctgcgtggcc gcccagccga tcttcgggag acggaccgac
     2101 aggtccccac cgaggcggaa gatgacatgg tcggtgcccc acgtctcgat gcggcgcagc
     2161 gggcggtcag cgaactgcgg gagctggtcg tcgagaagcc gacgcacaag gacgtcgtcg
     2221 acctcgacct cgtcctcgtg catccggggg accgccacgg gcgcccatgg gatcggcgag
     2281 ctccgtcgac gtcaaccgat ttcggtggcg cgcccaccgg ccacgccctc agcggtcagg
     2341 cgtcttcgta ctcgtcgtgg cggtggaacc atgagaagtc gccttcgttg cgaccgagcg
     2401 gagcccggtc gagccactgc cacatgcccc agaggccgtc ggtgccccgg gcataggccg
     2461 agtaggcgtg gaacacctca ccgtcatcga ggacgaaggt gctcagcccc ggcgactcgg
     2521 cttccgggaa tggctcgtcc gtctcgccat cgtggggcag ggcggcgacg tcgatggcga
     2581 ggggccgata attgtgctcc gcccccgcgg ccaactgcgc ctcggtgtag gacacgccga
     2641 agtcgaagtt gaagtcgctg cccatcgacg agacccacgg aaagctccag cccatccgcg
     2701 tccggtaggc cgtgatggcg tcgatcggcg ccctggacac ggtggccatg gccacgtcgt
     2761 gaccctcgag gtggactcga aagccgtcgt agccgtcggc caccgacgag cagctcgggc
     2821 acccctgggc cgggtccggg aacatgaagt ggtgtacgag cagctgcgag cgaccccgga
     2881 agaggtcccg cagcgtcacc tcaccgtctt cgccttcgaa gcggtagtcc ttctcgaccc
     2941 gaacccaggg caacgcctgt cgttgccggg ccacctcatc gctgcggcgg gtcagcgctt
     3001 tctcggcctc gagcagttcg agccgagccg cccgccatct ctcggcggtc tcgatggtgt
     3061 gtccggtcat ggtcttctcc tctacggcgc acgccgtgtg gcgtcacctg cccagaggac
     3121 gaagctgtga ctccgttctc gacatcggcc cgagacgcga cgccggccgt gccacgctct
     3181 ggcaatggag caggggtcgg tgtcgatgcg gctctacccg cacaacgacc tgggtgaccc
     3241 gaccgcgatc gtcgacgagc tgagggcgca agcccggctg gcggccgagg ccg
//
LOCUS       DASTFV010000327         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1214649, whole genome shotgun sequence.
ACCESSION   DASTFV010000327 DASTFV010000000
VERSION     DASTFV010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214649"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..>2736
                     /locus_tag="VFP02_06110"
     CDS             122..>2736
                     /locus_tag="VFP02_06110"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET9542633.1"
                     /translation="MEEALTLTFARLREASDAELRALEKRSARAAAVIDRFRDHRERL
                     EGHLDRHDLLVLAATAVRDGRAELNELGPVILHLPRRLGRSDLALLEALAARTEVVAV
                     LGVTGDPDGDRPLRRLVEQLEPVLGPAPAAPDVPDPVPARIVLVPDPDEEAREAVRTV
                     LARLEADPVDLDRIAIVSRVTSPYRLLLHEHLETAGLPHHTDLPWSVAQSTPGRVLLG
                     LLDLPEHSFRRADVARWLRSGPIRWQGRPVPASRWDAVARRSGVVQGLDQWRDRLDQR
                     RSELADAVEAGWSDAEYAESRTRQVDELQAFIAEIGGLLGAGRRSWSAWSAWSQEVLD
                     RYLLGEDGTIDWEADDLADLRDVRARVAAMADLDAVDGPADAGRFRRVLADELARTPR
                     RVGRLGRGVQVGDLESVYGLDLDLVVVVGMAEGWYPPRHRDDPLLPDHELGDAGLADA
                     LGRPDRSDERRDHLAARAAGADVVLLAPRSDPRGQRELQPARWLLEELSARTGWAVGV
                     GDVAELEVGGLERPRSFEESVRKVSVPLDGGERDLAALLASTAAGSPELHDHPVVAAD
                     PRLARGLEAVAARRDRTYGEWTGHIGARDELRIDDEALASATRLERFALCPFQYLLGN
                     VLGVRAHEDPVDEEGITPRDRGSLVHQVLEEFFAEALDRDPDAGWTDADLERLHGIVD
                     HVGEAYRARGMTGRDLGWELESSAIRRWLGAVLDRDADERSARQARPAAVELLFGGES
                     EHPAAEVGLPDGRRLRFKGAIDRVDREADGSLLVIDYKTGRGMSYKNVDDDRLDRGKR
                     LQLPIYADAARRALGGDGPPPPVEAYYWFVEEGGKKAWRGGTVDDAVQHRFEDVVATV
                     VDGIEGGDFPANPGE"
BASE COUNT          327 a          997 c         1055 g          357 t
ORIGIN      
        1 caccccactc gcctcggcgg gggatcggcg ccccctgacg gcgacgtggg gtgcggccct
       61 ggttcgggct ggtctgagcg aggcgcgacc gccgctgtcg caggtccccg caacggcggc
      121 ggtggaggag gcgctcacgc tcaccttcgc ccgcctgcgc gaggccagcg acgccgagct
      181 ccgggcgctg gagaagcgca gcgcccgggc cgctgcggtc atcgaccgct tccgggacca
      241 ccgcgaacgc ctggagggcc acctcgaccg ccatgacctg ctcgtcctgg cggccacggc
      301 cgtccgggac ggacgggctg agctgaacga gctcgggccg gtgatcctcc acctgccccg
      361 ccggctgggt cgttcggacc tcgccctgct cgaggccctg gccgcccgga cggaggtggt
      421 cgcggtcctc ggcgtcacgg gagatcccga tggtgaccga cccctccgcc ggttggtcga
      481 acagctggag cccgtcctcg gcccggcgcc ggcggcgccg gacgtgcccg accccgtccc
      541 ggcccggatc gtcctggtcc cggacccgga tgaggaggct cgggaggccg tgcggaccgt
      601 cctggcccgc ctggaggcgg atccggtgga cctcgaccgc atcgccatcg tctcccgggt
      661 gacgtcgccc tatcggctcc tgctccacga gcacctcgag acggcgggcc tgccccatca
      721 cacggacctc ccgtggtcgg tggcgcagag cacaccgggc cgagtgctcc tcggcctgtt
      781 ggacctgcct gagcacagct tccgtcgggc cgatgtcgcc cgttggctgc ggtccggccc
      841 catccgttgg caggggcgcc cggtgccggc ctcccggtgg gacgccgtcg cccgtcgttc
      901 cggtgtcgtc cagggcctcg accagtggcg tgaccggttg gaccagcgcc gctcggagct
      961 ggccgacgcc gtcgaggcgg gctggagcga cgccgagtac gccgagtcca ggacgcgcca
     1021 ggtcgacgag ctgcaggcgt tcatcgccga gatcggcggc ctgctcggcg cggggcgccg
     1081 atcgtggtcg gcgtggtcgg cgtggtcaca ggaggtcctg gaccgctacc tcctgggcga
     1141 ggacggcacc atcgactggg aagcggacga tctggccgac ctgcgcgacg tccgggcccg
     1201 ggtggcggcc atggccgacc tcgacgcggt cgacggaccg gccgacgccg gacggttccg
     1261 tcgggtcctg gccgatgagc tggcccgcac gccccggcgg gtgggccgcc tcggccgggg
     1321 cgtccaggtg ggcgacctcg agagcgtcta cggcctcgac ctcgacctgg tcgtcgtggt
     1381 gggcatggcc gagggctggt acccccctcg gcaccgggac gatccgctcc tgcccgacca
     1441 cgagctgggc gacgccggtc tggctgacgc cctcgggcgg cccgaccgct ccgacgaacg
     1501 tcgggaccac ctggccgcgc gcgccgccgg ggccgacgtg gtcctcttgg cccccaggag
     1561 cgacccgcgg ggccagcgcg agctccagcc cgcccgttgg ttgctcgagg agctcagcgc
     1621 ccgcaccggc tgggccgtcg gcgtcggtga cgtggccgag ctggaagtcg gcgggctgga
     1681 gcggccgagg tcgttcgagg agtcggtccg gaaggtgagc gtgccgctcg acggggggga
     1741 gcgggacctg gcggcgctcc tggcgtcgac cgcggccggg tccccggagt tgcacgatca
     1801 cccggtcgtg gcggccgacc cccgcctcgc ccgcggcctc gaggcggtcg cggcgcggcg
     1861 ggaccggacc tacggcgagt ggaccggcca catcggggcc cgggacgagc tgcgcatcga
     1921 cgacgaggcg ctggcgtcgg ccacccgact ggagcggttc gcgctctgcc cgttccagta
     1981 cctgctgggc aacgtcctgg gggtccgggc ccatgaggac cccgtcgacg aggagggcat
     2041 cacgccccgg gaccggggct cgctcgtgca ccaggtcctg gaggagttct tcgccgaggc
     2101 cctcgaccgt gatcccgacg cggggtggac cgacgccgac ctcgaacgac tccacgggat
     2161 cgtcgaccac gtcggcgagg cctaccgggc ccgggggatg accggccgtg acctgggttg
     2221 ggagctggag tcctcggcga tccgtcgctg gctgggcgcg gtcctggacc gggacgccga
     2281 cgagcggtcg gcccgtcagg cccgtccggc ggcggtcgag ctcctgttcg gcggggagag
     2341 cgagcacccc gcggccgagg tgggcctgcc cgacgggcgc cgactgcgct tcaagggggc
     2401 catcgaccgg gtggaccggg aggccgacgg atccctgctg gtgatcgact acaagaccgg
     2461 ccggggcatg tcgtacaaga acgtcgacga cgaccgcctc gaccggggca agcgcctgca
     2521 gctgcccatc tacgccgacg ccgcccgtag ggcgctcggt ggcgacgggc cgcctccgcc
     2581 cgtcgaggcc tactactggt tcgtcgagga gggtgggaag aaggcctggc ggggcgggac
     2641 ggtcgacgac gccgtccagc accgcttcga ggacgtcgtg gccaccgtgg tcgacggcat
     2701 cgagggcggc gacttccccg ccaacccggg cgagga
//
LOCUS       DASTFV010000328         4564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1218998, whole genome shotgun sequence.
ACCESSION   DASTFV010000328 DASTFV010000000
VERSION     DASTFV010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218998"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..828
                     /locus_tag="VFP02_06115"
     CDS             25..828
                     /locus_tag="VFP02_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HET9542634.1"
                     /translation="MAVGTTGTYEVATPVYDGPFELLLHLILREEVDLYEVSLLDIVD
                     AYLAEFERMERCDLDTATEFLLIASTLVELKSRRLLPVDDDVDLDDELALWEERDLLL
                     SRLLECKTFKDAAVVLRRLHRDGARTYPRLAGIEEQFVSLAPDLLAGVDADDVRRAYV
                     KVLTRPQPRVDLFHIAAIKVNVADAVEELVDELPRLGRTTFRSLTSSLVERLEVVVRF
                     LGLLELFKVGLVELDQVGQFGDIHIVWLGPAEDAATTGEVLESIDVYEG"
     gene            830..1480
                     /gene="scpB"
                     /locus_tag="VFP02_06120"
     CDS             830..1480
                     /gene="scpB"
                     /locus_tag="VFP02_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET9542635.1"
                     /translation="MAEEVHRAVEAIVMVAEAPVEPGLLAQLLELPVERIEEICSELA
                     LSYEAEDRGFQLQRVAGGYRYASHPDLAAYVERFVLEGQSTRLSSAALETLAIVAYKQ
                     PVSRVQVSAIRGVDADGVLRTLVQRGYVAEVARDPGPGQAVLYGTTPTFLERLGLDSL
                     TDLPPLGEFVPGAEVVEALEQGLRLPDEDDPLGADLDLTPGADGPEDPEGDPEPDA"
     gene            1458..2246
                     /locus_tag="VFP02_06125"
     CDS             1458..2246
                     /locus_tag="VFP02_06125"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET9542636.1"
                     /translation="MTPNPTPELPEGERLQKVLARAGFGSRRVCEDLIDEGRVTVDGD
                     VAVLGRRVDTATAQVEVDGVLIGVAPDLVHWLLHKPAKVVTTASDPEGRTTVLDLVPD
                     EPRVFPVGRLDYETEGLLLLTNDGSLTHRLTHPSFGVEKEYLAEVDRMPSRGDLRRLR
                     EGIELEDGLTAPAKVASLDDRTLRITIHEGRNRQVRRMVEAVGSTVLRLVRIRIGPLA
                     DRLLKPGEWRPLTQDEVRALERAVASAALPWEGRTEPDGTGPGA"
     gene            2273..2635
                     /gene="aroH"
                     /locus_tag="VFP02_06130"
     CDS             2273..2635
                     /gene="aroH"
                     /locus_tag="VFP02_06130"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET9542637.1"
                     /translation="MPLRALRGATTIDVDEVSHLTERMVELFTELFERNAIDHDDVVS
                     IIFTATDDIRCTFPATAARTFGLGDVPLICARELDVEGATPRCVRVMLHVETDRTRDQ
                     LHHVYQHGAANLRDDLPR"
     gene            2632..3699
                     /locus_tag="VFP02_06135"
     CDS             2632..3699
                     /locus_tag="VFP02_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HET9542638.1"
                     /translation="MSDPRRATLVGTGLIGGSIGLALRRAGWAVMGTDRDPDRAEAAV
                     AHGALDAVGEDATSDLTIVATPVGSVAGEARRLLDAGHRVVTDVGSVKAPLVAAVDHP
                     GFVGGHPMAGSEQEGIAAATADLFDGATWVLTPTATTDDQAYATVRAAIVELGAEVVA
                     LDPARHDALVAEVSHVPHLTAATLMRLADDTAEEHRALLRLAAGGFRDMTRVAAGHPG
                     IWPDICVENQAAIVDVLDRLITGLSEVRQVVADQDRAQLMSLLEQARSARTNLPARFG
                     RPSELGELRVIIPDRPGALAEVTTLATEIDVNIANMEIAHSSEGDRGVLILIVEAKEL
                     GRLHDALVERGFVVSHQQVLE"
     gene            3702..>4564
                     /locus_tag="VFP02_06140"
     CDS             3702..>4564
                     /locus_tag="VFP02_06140"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET9542639.1"
                     /translation="MSLTVGPSGPLRGRLRMPGDKSISHRALLLAALAEGRSRIEGLS
                     DGADVTGTRGAIETLGAATDDEDGAVVVDGGTLREPADVIDVGNSGTTIRLLTGLCSA
                     FPFLTVLAGDESIARRPMDRVAVPLRLMGASIEGREGGRLPPLSVRGGELHGITYRTP
                     VPSAQVKSAVLLAGLGAEGDTVVEEDVPTRAHTEELLARFGADVEVSEGGIRVRRSRP
                     EPFAYTVPGDPSQAAFWVVAATLVPGSDLVVEDLYLGPARTGYLDVLARMGADIEVER
                     DADAGTGTVRVR"
BASE COUNT          612 a         1686 c         1613 g          653 t
ORIGIN      
        1 caccccaccc gtatcctcgg ccgcatggct gtgggcacga cggggaccta cgaagtggcg
       61 acgcccgtct acgacgggcc cttcgaactg ctgctccacc tgatcctgcg cgaagaggtg
      121 gacctgtacg aggtcagcct gctcgacatc gtcgacgcgt acctggccga gttcgagcgg
      181 atggagcgct gcgacctcga cacggccacc gagttcctgc tcatcgcctc cacgctggtc
      241 gagttgaagt cccgccggct cctgccggtg gacgacgacg tggatctcga cgacgagttg
      301 gctctgtggg aggagcggga cctgctcctg tcccgcctgc tggagtgcaa gacgttcaag
      361 gacgccgccg tcgtcctgcg tcgcctgcac cgcgacggcg cgcgcaccta cccccgcctg
      421 gccggcatcg aggagcagtt cgtctccctg gcccctgatc tgctggccgg ggtcgacgcc
      481 gacgacgtgc gccgggccta cgtcaaggtg ctgacccgcc cccagccccg ggtcgacctc
      541 ttccacatcg cggcgatcaa ggtcaacgtg gccgatgccg tcgaggagct ggtcgacgag
      601 ctcccccgcc tcggccgcac cacgttccgg tcgctgacca gctccctggt cgagcgcctc
      661 gaggtggtcg tgcgcttcct cggcctcctc gagctcttca aggtcggcct ggtcgagttg
      721 gatcaggtgg gccagttcgg ggacatccac atcgtgtggc tcgggccggc cgaggacgcc
      781 gcgaccaccg gtgaggtcct cgagtccatc gacgtctacg agggctgagg tggccgagga
      841 ggtccatcgc gccgtcgagg ccatcgtgat ggtggctgaa gccccggtcg agcccggcct
      901 gctggcccag ctcctggagc tgcccgtcga gcggatcgag gagatctgct ccgagctcgc
      961 cctcagctac gaggccgagg accgcggctt ccaactccag cgggtggccg gcggctaccg
     1021 ctacgccagc catccggacc tggccgccta tgtcgagcgc ttcgtcctgg agggccagtc
     1081 gacgcgcctg tccagcgccg ccctcgagac gttggccatc gtggcctaca agcagccggt
     1141 gtcccgggtg caggtctcgg ccatccgtgg cgtcgacgcc gacggcgtgc tgcgcacgtt
     1201 ggtccagcgg ggctatgtcg ccgaggtggc tcgcgacccc ggcccgggcc aggccgtgct
     1261 ctacgggacg acgcccacct tcctcgagcg gctcggcctc gactcgctca ccgacctgcc
     1321 gcccttgggc gagttcgtgc ccggcgccga ggtcgtcgaa gccctcgagc agggcctgcg
     1381 cctgcccgac gaggacgatc cgctgggcgc cgatctcgac ctcacgccag gggcggacgg
     1441 ccccgaggac cccgagggtg accccgaacc cgacgcctga gctccccgag ggcgagcgcc
     1501 tccagaaggt cctggcccgg gccgggttcg gcagccggcg ggtctgtgag gacctgatcg
     1561 acgagggccg ggtcaccgtc gacggcgacg tcgcggtgct gggccgccgg gtcgacacgg
     1621 ccacggccca ggtcgaggtc gacggggtcc tcatcggggt cgcccccgac ctggtccact
     1681 ggctgctgca caagccggcc aaggtggtga ccacggcatc ggatccggag gggcggacca
     1741 cggtgctcga tctcgtgccc gacgagcccc gggtgttccc ggtcggccgc ctcgactacg
     1801 agaccgaggg cctgctgttg ctgaccaacg acgggtcact cacccatcgc ctgacccatc
     1861 cgtcctttgg cgtggagaag gagtacctgg ctgaggtcga ccgcatgccg tcgcggggcg
     1921 acctgcgccg cctgcgggag gggatcgagc tggaggacgg gctgaccgcg ccggccaagg
     1981 tcgcctccct cgacgaccgc acgctgcgca tcaccatcca cgaagggcgc aaccggcagg
     2041 tccggcgcat ggtcgaggcc gtgggcagca ccgtcctgcg cctggtccgc atccgcatcg
     2101 gccccctggc cgatcggctg ctcaagccgg gggagtggcg gcctctgacc caggacgagg
     2161 tccgggcgct cgagcgggcc gtggcctccg cggcgttgcc gtgggaaggg cgcaccgagc
     2221 cggacggaac cggtccgggg gcgtaagggc cccaccggta cgctccccgg cgatgccact
     2281 gcgggccctc cggggcgcca ccaccatcga cgtcgacgag gtcagccacc tgaccgagcg
     2341 catggtcgag ctcttcaccg agctcttcga gcgcaacgcc atcgaccacg acgacgtcgt
     2401 cagcatcatc ttcacggcca ccgacgacat ccgctgcacc tttccggcca ccgcggcgcg
     2461 gacgttcggc ctcggtgacg tcccgctcat ctgcgcccgc gagctggacg tcgagggggc
     2521 cacaccccgg tgcgtgcggg tgatgctgca cgtcgagacc gaccggaccc gggaccagct
     2581 ccaccacgtc taccagcacg gggccgccaa cctccgtgac gacctgcccc gttgagtgat
     2641 ccgcggcgcg ccacgctggt cggcaccggg ctgatcggcg gctccatcgg gctggccctg
     2701 cgccgggccg gttgggcggt catgggcacg gaccgggacc ccgaccgggc tgaggccgcg
     2761 gtggcccacg gggcgctgga cgcggtgggt gaggacgcga cctcggacct gacgatcgtg
     2821 gccacacccg tcggttcggt cgccggcgaa gcccggcgtc tgctggacgc cggtcaccgg
     2881 gtcgtgaccg atgtcggcag cgtgaaggcc ccgctcgtgg ccgcggtgga ccacccgggg
     2941 ttcgtcggtg gccaccccat ggcggggtcc gagcaggagg gaattgcggc cgccaccgca
     3001 gacctcttcg acggggccac ctgggtcctg acccccacgg ccacgacgga cgaccaggcc
     3061 tacgccaccg ttcgagccgc catcgtggag ctgggggccg aggtggtcgc cctggacccc
     3121 gcccgccacg acgccctggt cgcggaggtc tcccacgtgc cgcacctgac ggcggcgacg
     3181 ctcatgcgcc tcgccgatga caccgccgag gagcaccggg ccctgctccg gctggccgct
     3241 ggcggcttcc gcgacatgac ccgggtggcg gccggccacc ccggcatctg gcccgacatc
     3301 tgcgtggaga accaggccgc gatcgtcgac gtcctcgacc gtctcatcac cgggctgagc
     3361 gaggtccggc aggtagtggc cgaccaggac cgggcccaac tgatgtcgtt gctggagcag
     3421 gcccggtcgg cccgcacgaa ccttccggcc cgcttcggtc ggccgtccga gctgggcgag
     3481 ctccgggtca tcatccccga ccgccccggc gcgctggccg aggtcaccac gctggccacc
     3541 gagatcgacg tcaacatcgc caacatggag atcgcccact ccagcgaggg cgaccgcggt
     3601 gtgctcatcc tgatcgtcga ggccaaggag ctgggccggt tgcacgacgc cctcgtcgag
     3661 cgcggcttcg tcgtgtccca tcagcaggtg ctcgagtagt ggtgagcctg accgtcggcc
     3721 cgtccgggcc gctgcgggga cggctccgca tgcccggcga caagtcgatc tcccaccggg
     3781 ctctcctcct ggcggcgctg gccgagggtc ggtcccgcat cgaaggcctc tccgacggtg
     3841 cggacgtgac cgggacccgc ggcgcgatcg agaccttggg cgcggccacc gacgacgagg
     3901 acggcgccgt cgtcgtcgac ggcgggacgc tgcgggaacc cgcggacgtg atcgacgtgg
     3961 gcaactcggg tacgaccatc cgcctcctca ccgggctctg ctcggccttc ccgtttctca
     4021 ccgtcctggc cggcgacgag tcgatcgccc ggcggcccat ggaccgggtc gccgtgccgc
     4081 tccggctgat gggagcctcc atcgagggcc gggaaggcgg tcgcctgccc ccgctgtccg
     4141 tgcggggcgg tgagctccac ggcatcacct accgcacccc cgtgccgagc gcgcaggtga
     4201 agagtgccgt gctcctggcc ggcctcgggg ctgagggcga caccgtggtc gaagaggacg
     4261 tgcccacccg ggcccacacc gaggagctcc tggcccggtt cggggccgac gtcgaggtga
     4321 gtgagggcgg catccgggtg cgccgctccc gccccgagcc gttcgcctac accgtccccg
     4381 gcgacccgtc acaggccgcg ttctgggtgg tggcggccac gctcgtcccg ggtagcgacc
     4441 tggtcgtcga ggacctctac ctcggccccg cccggaccgg ctacttggac gtcttggcgc
     4501 gcatgggcgc tgacatcgag gtcgaacgcg acgccgacgc cgggacgggc acggtccggg
     4561 tccg
//
LOCUS       DASTFV010000329         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1224355, whole genome shotgun sequence.
ACCESSION   DASTFV010000329 DASTFV010000000
VERSION     DASTFV010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224355"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFP02_06145"
     CDS             complement(<1..500)
                     /locus_tag="VFP02_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9542640.1"
                     /translation="MQIPIGPLTFDVATAGPEDGPVVILLHGFPQTSACWRHQMPVLA
                     DAGYRVLAPDQRGYSPGARPSGVAAYHSSELVADVLGLADAVGAERFHLVGHDWGASI
                     TWQVAGRHGDRLLTMTALSVPHPLAYATALASRDTDQEQRSSYFPMFRAEGAEDGLLA
                     NDATGLR"
     gene            complement(505..1029)
                     /locus_tag="VFP02_06150"
     CDS             complement(505..1029)
                     /locus_tag="VFP02_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542641.1"
                     /translation="MAAPQYVPVKAIDDTRLYTSPPRRPGSWTQDRPAELLSGQPRGD
                     RLGSPGPDQGFALTLAERFRDRLALRPGESADDALAGSLTVALKRASLFGRAPVVHDL
                     TIAFTLFGFLDEQADDELVALRRRLFEEVANSHHYVEQRRIADLVPGPTLRLTPAEVK
                     DRYATDWRSLLTSD"
     gene            complement(1039..>2199)
                     /gene="cimA"
                     /locus_tag="VFP02_06155"
     CDS             complement(1039..>2199)
                     /gene="cimA"
                     /locus_tag="VFP02_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HET9542642.1"
                     /translation="EHFFDGYKANPEFSLRVLEAAAMAGADCLVLCDTNGGSLPFDVE
                     RIVGEVVDYVDTEVGVHLHDDTGCGVANALAGVRGGATQVQGTINGYGERTGNCNLTT
                     IIPDLSLKMGVATIPVERLERLTPVAHHVAELVNISPDPQQAFVGASAFAHKAGLHTS
                     AIARRPDAYEHVDPDLVGNGTRFVVSELAGRSTLELKAQEIGVEMDGPALSDVVDRLK
                     QLEYEGFHFEAADGSLELLMRDATGWTQPFFKLESFRVSVEHRAPADGSSGVHGAEIV
                     TEATVKVEVEGQRVIATAEGNGPVNALDEALRKAMTMAGAHSVLGGVHLTDYKVRVLD
                     TQKGTGAVTRVLIDSTDGERSWSTIGVSENIIEASWQALADSIIFGLLHNRA"
BASE COUNT          305 a          731 c          845 g          318 t
ORIGIN      
        1 cgcaggccgg tggcgtcgtt ggccagcagc ccgtcctcgg ccccctcggc ccggaacatc
       61 gggaagtagg acgatcgctg ctcctggtcg gtgtcgcggg acgccagggc cgtggcgtag
      121 gccagagggt ggggcacgga cagcgcggtc atcgtcagca ggcggtcgcc gtgccggccc
      181 gccacctgcc acgtgatgct ggcgccccag tcgtggccga cgaggtggaa gcgctcggcc
      241 ccgacggcat cggccagccc caggacgtcg gcgaccagtt cactggagtg gtaggcggcc
      301 accccagaag gtcgtgcccc gggcgagtag ccccgctggt cgggggccag cacccggtag
      361 ccggcgtcgg ccaggacggg catctggtgg cgccagcagg ccgaggtctg cgggaacccg
      421 tggagcagga tcacgacggg cccgtcctcg ggaccggccg tggccacgtc gaaggtcagg
      481 ggaccgatcg ggatctgcac gacgtcagtc cgacgtgagc aggctccgcc agtcggtggc
      541 gtagcgatcc ttgacctccg cgggagtcag gcggagggtc ggtccgggga cgaggtcggc
      601 gatccgacgc tgctcgacgt agtggtggct gttggccacc tcctcgaaga ggcggcggcg
      661 gagagcgacg agctcgtcat cggcctgctc gtcgaggaag ccgaagaggg tgaaggcgat
      721 ggtcaggtcg tggaccacgg gggcccggcc gaacagggac gcccgcttga gggccaccgt
      781 caggctgccg gccagggcgt cgtcggcgct ctcgccgggt cggagggcca ggcggtcccg
      841 gaagcgctcg gccagggtca gggcgaagcc ctggtccggc ccggggctgc cgaggcggtc
      901 gcctcggggc tggccggaca ggagctcggc gggccggtcc tgggtccagg agcccggccg
      961 ccggggcggt gaggtgtaga gccgggtgtc gtcgatcgcc ttgacgggga cgtactgcgg
     1021 agcggccacc cgccgacctt aggcccggtt gtgcagcagg ccgaagatga tggagtcggc
     1081 gagggcctgc caggaggcct cgatgatgtt ctcgctgacc ccgatggtcg accacgatcg
     1141 ctcgccgtcg gtcgagtcga tcagcacccg ggtcaccgca ccggtgccct tctgggtgtc
     1201 gaggacccgg accttgtaat cggtcaggtg gacgccgccc agcaccgagt gggccccggc
     1261 catggtcatg gccttgcgca atgcctcgtc cagggcgttg accgggccgt tgccctcggc
     1321 ggtggcgatg acccgctggc cctcgacctc caccttgacc gtcgcctcgg tgacgatctc
     1381 ggcgccgtgc acgccactgg agccgtcggc cggggcccgg tgctccaccg agacccggaa
     1441 ggactccagc ttgaagaagg gctgggtcca gcccgtggcg tcacgcatca ggagctcgag
     1501 ggagccgtcg gcggcctcga agtggaagcc ctcgtactcc agctgcttca gccggtcgac
     1561 gacatcggac agcgccgggc cgtccatctc cacaccgatc tcctgggcct tcagctcgag
     1621 ggtcgagcgt ccggccagct cggacaccac gaagcgggtg ccgttgccga ccaggtcggg
     1681 atcgacgtgc tcgtaggcgt cgggccggcg agcgatggcg ctggtgtgca ggcccgcctt
     1741 gtgggcgaac gccgaggccc cgacgaaggc ctgctggggg tccgggctga tgttcacgag
     1801 ctcggccacg tggtgggcga ccggggtcag gcgctcgagg cgctcgaccg ggatcgtggc
     1861 cacgcccatc ttcagggaga ggtcggggat gatcgtggtc aggttgcagt tgcccgtgcg
     1921 ctcgccgtag ccgttgatgg tcccctggac ctgggtggcc ccgccccgca ccccggcgag
     1981 ggcgttcgcc acaccgcacc cggtgtcgtc gtgcaggtgc acgccgacct cggtgtccac
     2041 gtagtccacg acctcgccca cgatgcgctc gacgtcgaag ggcagggagc cgccgttggt
     2101 gtcgcagagg acgaggcagt cggccccggc catggccgcg gcttcgagga cccggaggct
     2161 gaactccggg ttggccttgt agccgtcgaa gaagtgctc
//
LOCUS       DASTFV010000330         2473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1233028, whole genome shotgun sequence.
ACCESSION   DASTFV010000330 DASTFV010000000
VERSION     DASTFV010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233028"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VFP02_06160"
     CDS             <1..508
                     /locus_tag="VFP02_06160"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HET9542643.1"
                     /translation="QLHAGSWRGHNPSLTDRFGPDQGADIPKPMDYVGALKPLLDRFG
                     NESALTVVIYTLDESTYSRELAPLAGHYPALRLGPPWWFHDSPEGIRRFRSLVTETAG
                     FANTVGFNDDARSLLTIPARHDMVRRVDAGFLAGLVAEGRLRDDEAAEVAYDLAHGLA
                     RRAFGVDR"
     gene            505..1011
                     /locus_tag="VFP02_06165"
     CDS             505..1011
                     /locus_tag="VFP02_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET9542644.1"
                     /translation="MTAIVLVGLMGSGKSTVGRLVAEATGRELVDVDVAITARTGKTV
                     RQLWEEGGEAAYRSLEREEVLTTLGRSDVVLAAPGGVILDPTVRDALGDAFVAWLRAR
                     PDTLAARVRAGDHRPLLGENPEDDFATMDAERADLYAAVADITLDADRNSPDALAAEL
                     VERLPDAP"
     gene            1027..>2473
                     /gene="uidA"
                     /locus_tag="VFP02_06170"
     CDS             1027..>2473
                     /gene="uidA"
                     /locus_tag="VFP02_06170"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF007538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HET9542645.1"
                     /translation="MLRPQDNEAREAKRLDGVWDFVADPDGLGRDEGWWRGPLPDPRP
                     MPVPAAYNDVFVEPPVHEHVGDVWYQREVHVPRGWDGRRIVLRFDAATHRAVVWAGDQ
                     QVGEHEGGYTPFEADLTALAVPGEALRITIVVNNELTWTSIPTGVINDLPNGGRKQFV
                     FQDFFNYAGLIRSVWLCSTAPTHLSDVTVVTDLDGGTGIVRWSTVVEDGGIDIRVVLR
                     DAGGGVVAEGQGDEGELRVADAELWAPGRGYLYDLQVDVMDGDELADRYHQTVGIRTV
                     RVDGTRFLINGEPFYFRGFGMHEDLNVRGRGHDDASMVHDFELLAWLGANSFRTSHYP
                     YAEEVLDHADRLGIVVIDETAAVGLNLGVAGGLFGDVARTTYTEDTVGSVTQGVHRQA
                     IEELVARDKNHPSVVLWSIANEPESHSEEARTYFEPLFAAAREADPTRPVGFVNMMLA
                     PPDRCFLTELADVVMVNRYYGWYVDAGDLEAA"
BASE COUNT          358 a          895 c          873 g          347 t
ORIGIN      
        1 ccagctccac gccggctcgt ggcggggcca caacccgtcg ctcaccgacc ggttcggtcc
       61 ggaccagggg gcggacatcc cgaagccgat ggactacgtc ggggcactca agccgctgct
      121 cgaccggttc ggcaacgagt ccgccctgac cgtggtcatt tacacgctgg acgagagcac
      181 ctacagccga gagctcgccc cgctggcagg ccactatccc gccctccggc tggggcctcc
      241 atggtggttc catgacagcc ctgagggcat tcggcggttc cgctcgctgg tgacggagac
      301 ggccggcttc gccaacacgg tcgggttcaa cgacgacgcc cgctccctgc tgacgatccc
      361 ggcccgtcac gacatggtcc ggcgggtcga tgccggcttc ctcgccggcc tcgtcgccga
      421 gggtcgcctg cgcgacgacg aggcggccga ggtcgcctac gacctcgccc acggcctggc
      481 ccgtcgggcc ttcggggtcg accggtgacc gccatcgtgc tggtcgggct catggggtcg
      541 ggcaagtcca ccgtcggccg gctggtggcg gaggcgaccg gccgggagct ggtcgacgtc
      601 gacgtcgcca tcacggcccg gacaggcaag accgtccgcc agctctggga ggaggggggc
      661 gaggcggcct accgcagcct ggagcgcgag gaggtgctga ccacgctcgg tcgatccgac
      721 gtcgtgctgg ctgcacccgg gggcgtgatc ctggacccca ccgtgcggga cgccctgggc
      781 gacgcgttcg tagcctggct gcgagcccga ccggacaccc ttgcggcacg ggtccgcgcg
      841 ggcgaccacc ggcccctcct gggcgagaac cccgaggacg acttcgccac gatggacgcc
      901 gagcgggcgg acctctacgc cgccgtggcc gacatcacct tggacgccga ccgcaacagc
      961 cccgacgccc tggccgccga gctggtcgag cggctgcccg acgccccctg accccgagga
     1021 gagcccatgc tgcgaccaca ggacaacgag gcccgcgagg ccaaacgcct cgacggcgtg
     1081 tgggacttcg tcgccgatcc tgacggtctc ggccgcgatg aggggtggtg gcggggcccg
     1141 ctccccgacc cgcggcccat gcctgtgccc gcggcttaca acgacgtgtt cgtcgagccc
     1201 ccggtgcacg aacacgtcgg cgacgtctgg taccagcgcg aggtccacgt gccccggggc
     1261 tgggacggcc ggcgcatcgt cctgcgcttc gacgccgcca cccaccgcgc ggtggtgtgg
     1321 gccggtgacc agcaggtggg ggagcacgag ggcggctaca cgcccttcga ggccgacctc
     1381 accgcgctgg ccgtgcccgg tgaggcgctg cggatcacca tcgtggtcaa caacgagctc
     1441 acctggacgt cgatcccgac cggggtgatc aacgacctgc ccaacggcgg ccgcaagcag
     1501 ttcgtcttcc aggacttctt caactacgcc gggttgatcc gcagcgtctg gctgtgctcc
     1561 accgcgccga cccatctctc ggacgtgacg gtggtgacgg atctcgacgg cgggaccggc
     1621 atcgtccgct ggtcgacggt ggtggaggac gggggcatcg acatcagggt cgtcctccgg
     1681 gacgccggcg gcggggtcgt ggccgagggt cagggcgacg agggtgagct gcgggtcgcc
     1741 gacgccgagt tgtgggcgcc gggccgcggc tacctgtacg acctgcaggt cgacgtcatg
     1801 gacggtgacg agctggccga ccgctatcac cagacggtcg gcatccggac cgtgcgggtg
     1861 gacggcaccc gcttcctgat caacggcgag cccttctact tccgtggctt cggcatgcac
     1921 gaggacctca acgtccgggg ccggggccat gacgacgcca gcatggtgca tgacttcgag
     1981 ctcctggcct ggctgggggc gaactccttc cgcacctcgc actaccccta cgccgaggag
     2041 gtcctcgacc acgccgaccg gctcggcatc gtggtcatcg acgagaccgc cgccgtgggc
     2101 ctgaacctcg gggtcgccgg ggggctcttc ggagacgtgg cccgcacgac ctacacggag
     2161 gacacggtcg ggtcggtcac ccaaggggtc caccggcagg cgatcgagga gctcgtggcc
     2221 cgcgacaaga accacccgag cgtcgtcctc tggagcatcg ccaacgagcc cgagtcccac
     2281 agcgaggagg cgcgcaccta cttcgagccg ctcttcgccg ccgcccggga ggccgacccg
     2341 acccggcccg tgggcttcgt caacatgatg ctggccccgc cggatcgctg cttcctgacc
     2401 gagctggccg acgtcgtgat ggtcaaccgc tactacggct ggtacgtcga cgccggcgac
     2461 ctcgaggcgg ccg
//
LOCUS       DASTFV010000331         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1239687, whole genome shotgun sequence.
ACCESSION   DASTFV010000331 DASTFV010000000
VERSION     DASTFV010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239687"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFP02_06175"
     CDS             complement(<1..563)
                     /locus_tag="VFP02_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET9542646.1"
                     /translation="MSKPVTAPDIRHRKVRDGHDPLVMVTAYDAPGARIADEAGVDLI
                     LVGDSVAMVVLGYEDTLQVTIDDLAHHTAAVARARPRPLIVGDMPWMSYHVSPEETVR
                     NAAQLIRAGAGSVKLEGGQKRLRMIEAILDAEMPVMGHLGLTPQSIKAMGGFKVQAKA
                     HQAALDLVADAKALAHAGCFAVVLEGVP"
     gene            728..934
                     /locus_tag="VFP02_06180"
     CDS             728..934
                     /locus_tag="VFP02_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542647.1"
                     /translation="MTTGTCDSCGRDDEDVTEVVRVYVVPESWDQERQVTEAEGTERW
                     CFPCLTHYPHRVVGVEDDAGGQTA"
     gene            complement(922..1152)
                     /locus_tag="VFP02_06185"
     CDS             complement(922..1152)
                     /locus_tag="VFP02_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HET9542648.1"
                     /translation="MADALADRALDEPHPARLAPNHPAREAILAAHAAAMAADEPLYP
                     DPVTGLLVMTAAYLVERGECCDTGCRHCPYAV"
     gene            complement(1173..1499)
                     /locus_tag="VFP02_06190"
     CDS             complement(1173..1499)
                     /locus_tag="VFP02_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542649.1"
                     /translation="MRARGWSGGSEANPTYGLAITASDRDIVIDKSWDSVRLFLGDAA
                     DAVEVNLSAAFWKSCPEFRHPAIRTWFEELGLAPWRKGSPPAFGVRNIGENQFTVRRL
                     ERKSLI"
     gene            complement(1546..>2069)
                     /locus_tag="VFP02_06195"
     CDS             complement(1546..>2069)
                     /locus_tag="VFP02_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542650.1"
                     /translation="SGWEVAGKTGTTQSNKDAWFVGYTCNVTTAVWVGNVGGPGEEIA
                     PLPGEGGTLAAPLWHEFMERITSAGYVQNDCALADVTEFPGRTAFEDRQVDDTTSQPT
                     CPAGYALADLDGDGVFESCAQSSDDGEPPTATTEPPTATTEPPPPTTEPPPPTTTIPA
                     GPPGGVDLQRQRE"
BASE COUNT          295 a          706 c          763 g          305 t
ORIGIN      
        1 gggacgccct ccagcacgac ggcgaagcac ccggcgtggg ccagggcctt ggcgtcggcc
       61 accaggtcca gcgcggcctg gtgggccttg gcctgcacct tgaagccgcc catggccttg
      121 atggactgcg gggtcaggcc gaggtggccc atgaccggca tctcggcgtc gaggatggcc
      181 tcgatcatcc gcaggcgctt ctggccgccc tccagcttga ccgagccggc gccggcccgg
      241 atgagctggg cggcgttgcg cacggtctcc tcgggggaca cgtggtagct catccacggc
      301 atgtcgccca cgatgagcgg gcggggtcgg gcccgggcca cggcggccgt gtggtgcgcc
      361 agatcgtcga tggtgacctg gagggtgtcc tcgtagccga ggaccaccat ggcgaccgag
      421 tcgccgacca ggatcaggtc gacgccggcc tcatcggcga tgcgggcgcc gggcgcgtcg
      481 taggcggtga ccatgaccag ggggtcgtgt ccgtcccgga ccttgcggtg ccggatgtcg
      541 ggcgcggtga cgggcttgct catcgaagcc atctccttct cgtccagcgc acattcggct
      601 gccggcgatg gccgtcagct taccctggga cccggatgcc agaccagacg aatgagcggc
      661 ggagccgatc ggcgggccgg aatgagcccc gcaaggcgat gagcgccagc gaagagccct
      721 cagcacggtg accacgggca cgtgtgactc ctgcggacgg gacgacgagg acgtgaccga
      781 ggtcgtccgg gtctacgtcg tgcccgagtc ctgggaccag gagcgccagg tcaccgaagc
      841 cgagggcacg gagcgatggt gcttcccgtg cctgacccac tacccccatc gagtggtggg
      901 cgtcgaggac gacgccggcg gtcagaccgc ataggggcag tgccgacacc cggtgtcgca
      961 acactccccc cgttcgacca ggtaggccgc ggtcatcacg aggaggcccg tgaccgggtc
     1021 ggggtagagc ggctcgtccg cggccatcgc cgcggcgtgg gcggccagga tcgcctctcg
     1081 ggcggggtgg ttcggagcca ggcgagccgg gtggggctcg tcgagcgccc gatcggcgag
     1141 ggcatcggcc acgccgcgag gccagggacg actcagatga gcgacttgcg ctccaaccgg
     1201 cggaccgtga actggttctc gccgatgttg cgcacgccga acgccggcgg tgagcccttc
     1261 cgccacgggg ccaggcccag ctcctcgaac cacgtccgga tggccgggtg ccggaactcc
     1321 gggcagctct tccagaaggc ggcggacaga ttgacctcga cggcgtcggc ggcgtcgccg
     1381 aggaagaggc gcacgctgtc ccaggacttg tcgatcacga tgtcacggtc ggacgcggtg
     1441 atggccagcc cataggtggg attcgcctca ctgcctccag accaacctcg ggcacgcatc
     1501 gtcagctcct caacattcgt cggacaagta gatcacgtcg agccgtcatt cgcgctggcg
     1561 ctggaggtcg acccctccgg gcggaccagc cggaatggtc gtggtcggcg gcgggggctc
     1621 ggtcgtcggc ggcgggggct cggtcgtcgc cgtcgggggc tcggtcgtcg ccgtcggggg
     1681 ctcgccgtcg tcgctgctct gcgcgcagct ctcgaagacg ccgtcgccgt ccaggtcggc
     1741 cagggcgtaa cccgcggggc aggtgggctg ggacgtcgtg tcgtcgacct ggcggtcctc
     1801 gaacgcggtc cggcccggga actcggtcac gtcggccagc gcacagtcgt tctgcacgta
     1861 gccggccgag gtgatccgct ccatgaactc gtgccacagc ggtgccgcca aggtgccgcc
     1921 ctcgccagga aggggagcga tctcctcgcc cggcccgccg acgttgccga cccacaccgc
     1981 ggtggtgacg ttgcaggtgt accccacgaa ccaggcgtcc ttgttgctct gcgtggtccc
     2041 ggtcttgccg gccacctccc agcccgaca
//
LOCUS       DASTFV010000332         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1241780, whole genome shotgun sequence.
ACCESSION   DASTFV010000332 DASTFV010000000
VERSION     DASTFV010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241780"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..>2280
                     /locus_tag="VFP02_06200"
     CDS             18..>2280
                     /locus_tag="VFP02_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9542651.1"
                     /translation="MATQLADDLAQFLPADQVATFPAWETLPFERVSPGVETMGRRLR
                     VLWRLRDPGRAPDVVVSSVRALVQRLGPHVEDIEPVTVTPGLQVDRDDLVDDLVAAGY
                     RREEMVEHRGELAVRGSIVDVFPSTADRPVRIDLWGDEVDRLTEFSVTDQRSTTDLGR
                     VDIFPCRELLPTEDVRERAAALLASEPWGREQWDRLAEGQTFDGMESWLPWLAGAERL
                     LADLLPDNALMLLVEPARMRDRAADLLADETDLATTLAQTWEAGDRAFPQLHLDFDRL
                     LAHTDAPVWSLTPVPEGPSSPEVKASAWDPVIGDGAVLAKQLQGLLADDYRLVIAADG
                     DGSADRMVEVLAEHAVLVGRHTTDDDPLPAPRTGLATVAPLERGFILPGAKLAVLTER
                     DITGRRRAHRPARPRQRDARGFFDDLKEGDYVVHHQHGVARYGGMVRRAIAGHDRDYL
                     VLEYRSGDRLYVPSDQIDVVRHYTGGDSPSLSRLNGSDWQKTKARVRSAVAEIAQELV
                     VLYQKRITSPGTVFGLDTPWQHELEAAFPYRETPDQLAAIEAVKADMEAPAPMDRLVC
                     GDVGFGKTEVAIRAAFKAVQEGMQVAVLVPTTLLAQQHGQTFGDRFAPYPIRVEVLSR
                     FLTAGQQKAVIEGIGLGSVDLVIGTHRLLSGDVRFANLGLLIVDEEQRFGVSHKEAIK
                     EFRADVDVLTLSATPIPRTLEMSLTGIRDLSIINTPPADRQPILTYVGDYDERAVAES
                     IRRELLREGQVFFV"
BASE COUNT          320 a          868 c          784 g          308 t
ORIGIN      
        1 cctgcccgac ggggacgatg gccacacagc tcgccgacga cctcgcccag ttcctgccgg
       61 ccgaccaggt cgccacgttc ccggcgtggg agacgctgcc gttcgagcgg gtgagccccg
      121 gcgtcgagac catgggccgg cgcctgcggg tcctgtggcg cctgcgcgac cccgggcggg
      181 cgcccgacgt cgtcgtgtcc tccgtgcggg ccctcgtcca gcggctcggc ccccacgtcg
      241 aggacatcga gcccgtgacc gtcacgcccg ggctccaggt cgaccgcgac gacctcgtcg
      301 acgacctggt ggccgccggc taccgccggg aggagatggt cgagcaccgc ggcgagctgg
      361 ccgtgcgggg ctcgatcgtc gacgtgttcc cctccaccgc cgaccggccg gtgcgcatcg
      421 acctgtgggg cgacgaggtc gaccgcctga ccgagttctc ggtcaccgac cagcggtcca
      481 ccaccgatct gggtcgggtc gacatcttcc cgtgccgcga gctgctgccc accgaagacg
      541 tccgggagcg ggccgccgcc ctcctggcgt ccgagccctg gggtcgggag cagtgggacc
      601 gcctggccga gggccagacc ttcgacggga tggagtcgtg gctgccttgg ctggcggggg
      661 cggagcggct gctcgccgac ctgctcccgg acaacgccct catgctcctg gtcgagcccg
      721 cccgcatgcg agaccgagcg gccgacctgc tggccgacga gaccgacctg gccaccacgc
      781 tggcccagac ctgggaggca ggggaccggg ccttcccgca gctccacctc gacttcgacc
      841 gcctgctggc ccacaccgac gcccccgtct ggtcgctcac gcccgtgccc gagggtccgt
      901 cgtcgcccga ggtcaaggcg tcggcgtggg accccgtcat cggcgacggg gcggtgctgg
      961 ccaagcaact gcagggcctc ctggccgacg actaccggct cgtgatcgcg gctgatggtg
     1021 acggcagcgc cgaccgcatg gtcgaagtcc tcgcggagca cgccgtcctc gtcggtcgcc
     1081 acaccacgga cgacgacccg ctgcctgcgc cccgcacggg cctcgccacc gtggcccccc
     1141 tggagcgtgg gttcatcctc ccgggggcga agctggccgt cctcaccgag cgggacatca
     1201 ccggccggcg ccgggcccac cgtcccgccc gcccgcgcca gcgcgacgcc cggggcttct
     1261 tcgacgacct caaagagggc gactacgtcg tccaccacca gcacggcgtg gcccgctacg
     1321 gcggcatggt ccgccgcgcc atcgccggcc acgaccggga ctacctggtc ctggagtacc
     1381 gcagcggcga ccggctgtac gtcccgtcgg accagatcga cgtggtccgt cattacaccg
     1441 gtggggacag cccgtccctg agccggctca acgggtcgga ctggcagaag acgaaggccc
     1501 gggtgcgctc ggcggtcgcg gagatcgccc aagagctggt cgtcctctac cagaagcgca
     1561 tcaccagccc gggcacggtc ttcgggctcg acacgccgtg gcagcacgag ctcgaggccg
     1621 ccttccccta ccgggagacc ccggaccagc tcgccgccat cgaggcggtc aaggcggaca
     1681 tggaggcgcc ggcccccatg gatcggctgg tctgcggcga cgtcggcttc ggcaagaccg
     1741 aggtcgccat ccgggccgcg ttcaaggcgg tccaggaggg catgcaggtc gcggtcctgg
     1801 tgccgaccac cctgctggcc cagcagcacg gtcagacgtt cggcgaccgt ttcgcgccgt
     1861 acccgatccg ggtcgaggtc ctcagccgct tcctgaccgc cggccagcag aaggcggtca
     1921 tcgagggcat cggactgggg tccgtcgacc tggtcatcgg cacccaccgc ctgctgtcgg
     1981 gcgacgtccg attcgccaac ctgggcctgc tgatcgtgga cgaggagcag cgcttcggcg
     2041 tcagccacaa ggaggccata aaggagttcc gggccgacgt ggacgtgctc accctgagcg
     2101 ccacaccgat cccacggacc ctggagatga gcctcacggg gatccgggac ctgtcgatca
     2161 tcaacacccc gccggccgac cgccagccca tcctcaccta cgtcggggac tacgacgagc
     2221 gggccgtcgc cgagtccatc cgccgggagc tgctccgcga gggccaagtc ttcttcgtcc
//
LOCUS       DASTFV010000333         6546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1244466, whole genome shotgun sequence.
ACCESSION   DASTFV010000333 DASTFV010000000
VERSION     DASTFV010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244466"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..781
                     /locus_tag="VFP02_06205"
     CDS             197..781
                     /locus_tag="VFP02_06205"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HET9542652.1"
                     /translation="MSDTDEQQPETQSDDGIPQTLVGISFADIFRAQEFLTAATGLAA
                     KGRLKLKDAVLVVKDADGKTHVKETVDPELGRTAMSGALWASLFGLVLGGPVGWAAGL
                     AVGAGAGAVTAKVVDLGISDEWVSWFREAVQPDTATVALLIEDLDRNALVEEAARFTG
                     AELVYANLDDDTLERIKSALEGQVTGSERPGSDV"
     gene            830..2296
                     /locus_tag="VFP02_06210"
     CDS             830..2296
                     /locus_tag="VFP02_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9542653.1"
                     /translation="MRILVMGATGYIGGRLVPELADAGHEIRCLTRRPEKLAGVPWAE
                     RVEVVQGDALDPASLTEALADVEVVYYLVHSIGSGGHFEDTDREAANNVAAAAKAAGT
                     ERIVYLGGLVPGAEETASAHLASRAEVGQIFLDSGVPTVVLQAGVIIGSGSASFEMLR
                     YLTERLPVMVTPRWVSSRVQPIAVRDVLTYLVGVLDLPDGTNRRFDVGGPDVLTYREM
                     MRRYAAVAGLRHRMIIPVRVLSPWLSSQWVNLVTPVPKAIAQPLIESLRNSVVVQEDD
                     ITALVPADRLGFDEAVRLALEQIRTGDVATRWSGADWPGAPSDPMPTDPDWSGGTVYR
                     DERSAPVDASVDQLWQAVEGIGGDRGWYSFPLAWAVRGLLDRLAGGVGLRRGRRDPDR
                     TAVGEPIDFWRVETLERGKVLRLRAEMRLPGGAWLEFAITPDEGDGPVLHQRALFIPR
                     GLAGTLYWWSVWPFHGIVFGSMLRNLAKAAEAQPQLTR"
     gene            complement(2301..3227)
                     /locus_tag="VFP02_06215"
     CDS             complement(2301..3227)
                     /locus_tag="VFP02_06215"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET9542654.1"
                     /translation="MSGPVETCDAKADPAGGPVIAVEPSREARVGDTTVRRALPRRGH
                     RTVGAWCFADHMGPVADGGGLGTGIGPHPHIGLQTVTWLVEGEMLHRDSLGAEQLIRP
                     GQLNLMTAGRGVAHAEEGTAESAGKPKHGIQLWVAQPDATRQGEPAFEHHADLPRVAV
                     DSGEATVLVGELAGVTSPARADTDHVGAELTLRRGNTIVPLRPEHEHALVVLEGAVDL
                     GDDTVVEPGFLAYLGTGRDELPMAMTDAARVMLLGGTPFESEISMWWNFVARTHDEMD
                     EARSAWEAGDEDRFGPVSSTLGWIAAPTTPWR"
     gene            complement(3224..3649)
                     /locus_tag="VFP02_06220"
     CDS             complement(3224..3649)
                     /locus_tag="VFP02_06220"
                     /inference="COORDINATES: protein motif:HMM:NF016211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HET9542655.1"
                     /translation="MTETPVDRSRTVRAGWLAMGLVCVGLGGLGIVLPGLPTTVFFIA
                     AAACFSKSSPRLEAWVLGLRGVGPMVRDHRAGLGMPRRAKVLATACIVLFVGLALWLA
                     IEHPALRGVVAAAGLVGVVVIWWRVPTREAVLRTQEVGT"
     gene            complement(3646..3984)
                     /locus_tag="VFP02_06225"
     CDS             complement(3646..3984)
                     /locus_tag="VFP02_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542656.1"
                     /translation="MSETTAVPAAAVEYTPAQAEKAFSKSVMVSAVRCLFTYIVFPWL
                     LPALGVARGWGPWLGVVVSPIAIAFNVLSIRRFQRSGHRYRWPITVVNATIIVLLVVL
                     FVRDVADILS"
     gene            complement(4113..4760)
                     /locus_tag="VFP02_06230"
     CDS             complement(4113..4760)
                     /locus_tag="VFP02_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HET9542657.1"
                     /translation="MFALGLVAAACGDDSESSESASDATAAPADDTTAPDDTEMTDDT
                     TPVDSSTICDTDGLVEAVESGPEDGTLAGMADDPVGTAASSNPVLTTLVAAVTEADLV
                     DTLNSAEELTVFAPTDCAFAELDPATLEAAMADPTGLLTTVLGFHVVPQRLSAADLTD
                     GMELETFTGETLVVAVDGDTISLNDGGATVQVPDVETANATVFLIDSVMIPPSVG"
     gene            complement(4881..6479)
                     /locus_tag="VFP02_06235"
     CDS             complement(4881..6479)
                     /locus_tag="VFP02_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9542658.1"
                     /translation="MSEQEERVSKGDRALFALSGLVAAAVGLGAAELSTSLNRAWRSP
                     VEVVAEVVIDRAPQSITHFGIELFGRNDKKALVIGILVTLTIVGLVVGALSRRRPVVG
                     DIAFGAFAVVGAGAALQVANAPLSAVIPSLIAGGLGAVTLRFLRARLLNVEAARAEKP
                     VSDETTGQPAAEPVGGTSRRTFLALTGAMVALAGVAAAGGRALRDRFSAADSRNNLVL
                     PAPDETAPPITDAYSVDVEGMTPFRTSNADFYRIDTALTVPQVPVEGWELEVKGMVDE
                     PLRLTFDELVAFGLVEADVTMTCVSNRVGGELIGNARWLGVPVQRLLDEVGVDPEADQ
                     LVGRSVDGYTCGFPVSAVGDGRTCLVAVGMNGEPLPLEHGFPARLVTAGLYGYISATK
                     WLTEIELTTFDAFDSYWVPRGYADRAPIKSECRIDVPRTGEPVTAGPQSIAGVAWAQT
                     RGIQKVEVKVDDGPWQEAELADALTDETWRQWRIDWDAQPGSPRIMCRATDGDGELMT
                     EERSEPLPDGATGWQSKLVIVSAA"
BASE COUNT          921 a         2339 c         2400 g          886 t
ORIGIN      
        1 ctccgcggcc tcgatggcgg cgcaggtgcc ggccacgccg aggccgacca ccaccacgtc
       61 ggcgcggtcg tcccaggacc cgacatcggc ggcggcgaga ggaaggatcg gttcggtgga
      121 gaggcccggc acggcgtcgg accgtaacgc aggactgccc ggtgctcggg acggcggcag
      181 ccgtagcctt cgaggggtga gtgacaccga cgagcagcag cccgagaccc agagcgacga
      241 cgggatcccc cagaccctcg tcggcatctc gttcgccgac atcttccggg cccaggagtt
      301 cctcaccgcg gccacgggcc tggccgccaa gggccgcctg aagctcaagg acgccgtgct
      361 cgtcgtgaag gacgccgacg gcaagaccca cgtgaaggag accgtcgacc ccgagctcgg
      421 tcgtaccgcc atgtcgggcg ccctctgggc cagcctgttc gggctcgtgc tcggcggccc
      481 ggtgggttgg gccgccggcc tcgccgtcgg tgccggcgcc ggggcggtca cggccaaggt
      541 ggtcgacctc ggcatctccg acgagtgggt cagctggttc cgggaagcgg tccaacccga
      601 caccgccacc gtcgctctgc tcatcgagga tctcgaccgc aacgctctgg tcgaggaggc
      661 cgcccgcttc accggcgccg agctggtcta cgccaacctc gacgacgaca ccctcgagcg
      721 catcaagtcc gcgctcgagg gccaggtcac cgggagcgag cgccccggct ccgacgtctg
      781 aatccagcag gggccggggc tccggcgacc gcatccctag cctgagcggg tgaggatcct
      841 cgtcatgggc gccaccggct acatcggggg gcgcttggtc cctgagctcg ccgacgccgg
      901 tcacgagatc cggtgcctga cccggcgacc ggagaagctg gccggggtcc cgtgggccga
      961 gcgggtcgag gtcgtccagg gtgacgccct cgaccccgcg tccctgaccg aggcgctggc
     1021 ggacgtcgag gtcgtctact acctggtcca ctccatcggg tccggtggcc acttcgagga
     1081 cacggaccga gaggcggcga acaacgtggc cgccgcggcc aaggcggcgg gcaccgagcg
     1141 catcgtctac ctcggcgggc tcgtcccggg cgcggaagag acggcgtcgg cccacctggc
     1201 ctcccgagcc gaggtcggcc agatcttcct ggactccggc gtccccaccg tggtgctcca
     1261 ggccggtgtc atcatcggca gcggcagcgc ctccttcgag atgctgcgct acctgaccga
     1321 gcggttgccg gtcatggtca ctccgcgctg ggtgtcgtcc cgtgtgcagc cgatcgccgt
     1381 gcgcgacgta ctcacctacc tcgtcggtgt cctggacctg cccgacggca cgaaccggcg
     1441 cttcgacgtc ggcggcccgg acgtcctgac ctaccgggag atgatgcgcc gctacgccgc
     1501 tgtggccggg ctgcgtcacc ggatgatcat ccccgtccgg gtcctgagcc cctggctctc
     1561 gagtcagtgg gtgaacctgg tcacgcccgt gccgaaggcc atcgcccaac ccctcatcga
     1621 gtcgttgcgg aactcggtcg tggtccagga ggacgacatc accgcgctcg tcccggccga
     1681 ccgcctcggc ttcgacgagg ccgtgcgctt ggccctggag cagatccgga cgggtgacgt
     1741 cgccacccgg tggtctggag ccgactggcc cggcgccccg agcgacccca tgccgaccga
     1801 tcccgactgg agcggcggga ccgtttaccg cgacgagcgg tcggccccgg tcgacgcctc
     1861 ggtggaccag ctctggcagg ccgtcgaggg catcggcggc gaccgcggct ggtactcctt
     1921 tcctctggcc tgggcggtcc gcggcctcct cgaccgcctc gccggcggcg tcggcctccg
     1981 tcggggccgg cgcgacccgg atcgcacggc ggtgggtgag cccatcgact tctggcgggt
     2041 ggagaccctc gagcggggaa aggtgttgcg cctgcgggcc gagatgcgcc tacccggcgg
     2101 ggcctggctg gagttcgcga tcaccccgga cgagggcgac ggtcctgtcc tccaccagcg
     2161 ggccctcttc atcccccggg gcttggccgg caccctctac tggtggtcgg tatggccctt
     2221 ccacggcatc gtcttcgggt ccatgctgcg caacctggcc aaggccgccg aggcgcagcc
     2281 ccaactcacc cgttgagagc tcagcgccag ggggtggtgg gggcggcgat ccagcccagg
     2341 gtggaggaga cggggccgaa gcggtcctcg tcgccggcct cccaggccga ccgggcctcg
     2401 tccatctcgt catgggtgcg ggcgacgaag ttccaccaca tggagatctc ggactcgaag
     2461 ggcgtcccgc ccagcagcat cacccgggct gcgtcggtca tggccatcgg gagctcgtcc
     2521 cggccggtgc cgaggtaggc gaggaacccg ggctcgacga ccgtgtcgtc gcccaggtcg
     2581 acggcgccct ccagcacgac gagcgcgtgc tcgtgctcgg gccgcagggg cacgatcgtg
     2641 ttccctcggc ggagggtcag ctcggccccg acgtggtcgg tgtcggcccg agccggtgag
     2701 gtgacaccgg ccagctcgcc gaccaagacg gtggcctcgc cggagtcgac cgccacccgg
     2761 ggcagatcgg cgtggtgctc gaaggcgggc tcgccctgtc gggtcgcgtc gggctgggcc
     2821 acccagagct ggatgccgtg cttcggcttc cccgccgact cagcggtccc ctcctcggcg
     2881 tgggccacgc ctcggcccgc cgtcatcagg ttgagctggc cgggtcggat cagctgctcg
     2941 gcgccgaggc tgtcccgatg cagcatctcg ccctcgacca gccaggtgac cgtctgcagg
     3001 ccgatgtggg ggtgggggcc gatgccggtg cccagcccgc caccgtcggc cacggggccc
     3061 atgtggtcgg cgaagcacca ggccccgacc gtgcggtgac cccgccgggg cagggcccgc
     3121 cggaccgtgg tgtccccgac ccgggcctcc cggctgggct cgacggcgat gaccgggccg
     3181 ccggccgggt cggccttcgc gtcgcacgtc tcgaccggtc cgctcatgtc ccgacctcct
     3241 gggtccgcag cacggcctca cgggtgggga cgcgccacca gatcacgacc acaccgacca
     3301 ggcccgcggc ggcgaccacg cctcgcaggg cggggtgctc gatggccagc cacagggcca
     3361 gcccgacgaa gagcacgatg caggccgtgg ccagcacctt ggcccgacgg ggcatgccca
     3421 ggccggcccg gtggtcccgc accatggggc ccacgccccg caagccgagc acccaggcct
     3481 cgaggcgggg gctcgacttg gagaagcagg ccgcggcggc gatgaagaag accgtcgtgg
     3541 gcaggccggg gagcacgatg cccaaccccc cgaggccgac gcacacgagc cccatggcca
     3601 gccaacccgc ccggacggtc cgggaccggt cgacgggcgt ctcggtcacg acaggatgtc
     3661 ggcgacgtcc cggacgaaca gcaccaccag caacacgatg atcgtggcgt tgaccacggt
     3721 gatcggccag cggtacctgt gcccgctgcg ctggaagcgg cggatgctca gcacgttgaa
     3781 cgcgatggcg atcgggctca ccaccacgcc gagccacggg ccccagcccc gggccacccc
     3841 gagggcgggc agcagccacg ggaagacgat gtaggtgaac aggcagcgga cggccgagac
     3901 catcacggac ttgctgaacg ccttctccgc ctgggccggc gtgtactcga ccgccgccgc
     3961 cggcaccgcc gtcgtctcgc tcacggcccc aagtctggcc gacgctcggc cactccgcac
     4021 catcagcccg gacgacttcc ccggcacacc ccgcacgcga cggggccccg ggtcggatcg
     4081 acccggggcc ccgtccgctc acgagggaac ggtcagccca cgctcggcgg gatcatcacg
     4141 ctgtcgatga ggaacaccgt ggcgttggcc gtctcgacgt cgggcacctg cacggtggca
     4201 ccaccgtcgt tcagcgagat ggtgtcgccg tcgaccgcca cgacgagggt ctcgccggtg
     4261 aaggtctcca gctccatgcc gtcggtcagg tcagcggcgg agagccgctg ggggacgacg
     4321 tggaagccca ggaccgtggt cagcagaccg gtcgggtcgg ccatggcggc ctcgagggtg
     4381 gcggggtcca gctcggcgaa cgcgcagtcc gtcggggcga acaccgtcag ctcctcggcg
     4441 ctgttcaggg tgtcgaccag gtcggcctcc gtcacggcgg cgaccagggt ggtcagcacg
     4501 gggttgctgg aggcggcggt gccgacgggg tcgtcggcca tgccggccag cgtcccgtcc
     4561 tcgggaccgg actcgacggc ctcgacgagc ccatcggtgt cgcagatggt ggacgagtcc
     4621 accggggtgg tgtcgtcggt catctcggtg tcatccggcg ccgtcgtgtc gtcggccgga
     4681 gccgccgtcg cgtcggacgc ggattcgctc gactcggagt cgtcgccaca cgcggcggcg
     4741 accagaccga gagcgaacac cagagccagg attcgaatca gcttcgaacg gaacatggct
     4801 gtcacacact cactttcttg ggttgggggg ttgctgttgg gaactcgccc gggatcgggc
     4861 aagagggggt cggggcgtgg tcaggccgcc gaaacgatca cgagcttcga ttgccagccg
     4921 gtggccccgt cggggagcgg ctcactgcgc tcctcggtca tgagctcgcc gtcaccgtcg
     4981 gtggcccggc acatgatccg gggcgagccc ggctgggcgt cccagtcgat gcgccactgc
     5041 cgccaggtct cgtcggtcag ggcgtcggcc agctcggcct cctgccacgg gccgtcgtcg
     5101 accttgacct cgaccttctg gatgcctcgg gtctgggccc aggccacgcc ggcgatcgac
     5161 tgcggaccgg ccgtgaccgg ctcaccggtg cgagggacgt cgatgcggca ctcggacttg
     5221 atcggggccc ggtcggcgta gccccggggc acccagtagg agtcgaaggc gtcgaaggtg
     5281 gtcagctcga tctcggtgag ccatttcgtc gccgagatgt agccgtagag gccggcggtc
     5341 accaggcggg cggggaagcc gtgctcgagg ggcaggggct cgccgttcat gccgacggcg
     5401 accaggcagg tgcggccgtc gcccacggca ctgaccggga agccgcaggt gtagccgtcg
     5461 acggagcgac ccacgagctg gtcggcctcg gggtcgaccc cgacctcgtc gagcaggcgc
     5521 tggaccggga cgccgagcca gcgggcgttg ccgatgagct cgcccccgac ccggttggag
     5581 acgcaggtca tggtcacgtc ggcctcgacc aggccgaagg cgacgagctc gtcgaaggtc
     5641 aggcgcaggg gctcgtcgac catgcccttg acctccagct cccagccctc cacggggacc
     5701 tggggcacgg tgagggcggt gtcgatccgg tagaagtcgg cgttggacgt gcggaagggg
     5761 gtcatcccct cgacgtcgac cgagtaggcg tcggtgatgg gcggggccgt ctcgtccggc
     5821 gccgggagga cgaggttgtt gcgggagtcg gcggcgctga aacggtcccg cagggcccga
     5881 ccgccggcgg cggccacgcc ggcgagggcc accatcgccc cggtcagggc caggaaggta
     5941 cggcggctcg tcccgccgac gggctcggcc gccggctggc cggtggtctc gtcgctcacc
     6001 ggcttctcgg cccgagctgc ctcgacattg aggagccggg cccgcaggaa gcgcagggtc
     6061 accgcgccga gaccaccggc gatcagcgag gggatgacgg cggacagcgg ggcgttggcc
     6121 acctggaggg cggcgccggc gccgacgacg gcgaaggccc cgaaggcgat gtcgccgacg
     6181 accggccgcc ggcggctgag cgcccccacg accaggccga cgatggtcag cgtgaccagg
     6241 atcccgatga ccagggcctt cttgtcgttg cggccgaaga gctcgatgcc gaagtgggtg
     6301 atgctctgcg gggcccggtc gatgacgacc tccgccacca cctcgaccgg cgagcgccag
     6361 gcccggttga gcgaggtcga gagctcggcc gccccgaggc cgacggcggc ggccaccagg
     6421 ccgctgaggg cgaagagggc ccgatcgccc ttcgagactc gctcctcctg ctcgctcacg
     6481 gggggtactc cggagccctc cccccgcgtg gatgggagaa atgggtcggt gcccccgaac
     6541 gggtga
//
LOCUS       DASTFV010000334         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1245348, whole genome shotgun sequence.
ACCESSION   DASTFV010000334 DASTFV010000000
VERSION     DASTFV010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245348"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /gene="nadA"
                     /locus_tag="VFP02_06240"
     CDS             <1..221
                     /gene="nadA"
                     /locus_tag="VFP02_06240"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HET9542659.1"
                     /translation="LTNRLNAETPGKTVLSLDPLVCPCSTMFRIDGPHLAWVLENLVD
                     GNVVNRITVDPETSEWARVALDRMLEIT"
     gene            322..1293
                     /gene="pseB"
                     /locus_tag="VFP02_06245"
     CDS             322..1293
                     /gene="pseB"
                     /locus_tag="VFP02_06245"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HET9542660.1"
                     /translation="MDLSGQTILLTGGTGSFGYAFVERLLADHDDVTIRVYSRDELKQ
                     SEMKARFGDAQVRYMLGDIRNRTRMARACQGVDMIVHAAAMKQVPACEYNPFEAVQTN
                     VLGAQHVVDCAIDAGVKQVVALSTDKAVNPVNLYGATKLCEEKIIVQGNAYAAAFDTR
                     LSCVRYGNVVGSRGSVVPLFKEQLKTGKLTLTDERMTRFWITLPEAVDLVLFAMENAK
                     GGEIFIPKIPSMRMTDLAEAMAPGVPTEIIGIRPGEKLHEVLLTDDEARHAIETEDNY
                     VVLPEHPWWTAEHPWIEGKPLDDDFVYASNTNHWWLTSDELQQRIVA"
     gene            1298..2377
                     /locus_tag="VFP02_06250"
     CDS             1298..2377
                     /locus_tag="VFP02_06250"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9542661.1"
                     /translation="MIPYGRQSVDDDDIDAVVRVLKGDCLTRGPAVEAFEHALAERVE
                     ARHAVAFSSGTAALHGAAAVAGLGPGDRVATSSLSFAASAACAAYVGATPVYVDIDPT
                     TMNFDPGAVPDGIAALVAVHYAGLPVDLAALNHRPPVVIEDAAQALGARTPDGPVGNC
                     ARSDMACFSFHPVKSITTGEGGAVTTNDDDLADRLRAFRHHGLRPVPEEGPWATRIDE
                     IGFNYRLTDLQAALGTSQLHKLNRFVARREELARRYDDALADLDVVCPATAPAGTVHA
                     RHLYPILVENRANVVAELHRRAIGVQVHHVPIHHHPAYAAGTTPDDLPVTEAVYAHVL
                     SLPLFYDLTVDEQDTVVTALRRILS"
BASE COUNT          396 a          953 c          748 g          365 t
ORIGIN      
        1 acctgaccaa ccgcctcaac gccgagaccc cgggcaagac cgtgctctcc ctcgatccgc
       61 tggtctgccc ctgctcgacc atgttccgca tcgacggccc ccacctggcc tgggtcctcg
      121 agaacctggt cgacggcaac gtggtgaacc ggatcacggt cgaccccgaa accagcgaat
      181 gggcccgggt ggccctcgac cggatgctgg agatcacctg atccgacggt gggtgctcag
      241 caccaccacg cagagagaaa ccggacggga ggtgggtcgt acgacctaac cgcggggcat
      301 ccgtcggccg atggaacggg catggacctc tccggtcaga ccatcctcct cacgggcggc
      361 accggctcct tcggctacgc cttcgtggag cggctgctcg ccgaccacga cgacgtgacc
      421 attcgggtct actcccgcga cgagctgaag cagtcggaga tgaaggctcg cttcggcgac
      481 gcgcaggtcc gttacatgct gggcgacatc cgcaaccgga cccgcatggc ccgggcctgc
      541 cagggcgtcg acatgatcgt tcacgccgcg gccatgaagc aggtgccggc ctgtgagtac
      601 aaccctttcg aggccgtgca gaccaacgtg ctcggggccc agcacgtcgt ggactgcgcc
      661 atcgatgccg gggtcaagca ggtcgtggcc ctgtcgaccg acaaggccgt gaacccggtc
      721 aacctctacg gcgccaccaa gctctgcgag gagaagatca tcgtccaggg caacgcctac
      781 gccgccgcct tcgacacccg cctctcctgt gtccgctacg gcaacgtcgt cggttcccgg
      841 ggctcggtcg tccctctgtt caaggagcag ctcaagaccg ggaagctcac cctcaccgac
      901 gagcggatga cccgcttctg gatcaccctg cccgaggcgg tcgacctggt gctgttcgcc
      961 atggagaacg ccaagggggg cgagatcttc atccccaaga tcccgtcgat gcgaatgacc
     1021 gacctggccg aggccatggc ccccggggtc cccaccgaga tcatcggcat ccggcccggc
     1081 gagaagctgc acgaggtcct gctcaccgac gacgaggccc gccacgccat cgagaccgag
     1141 gacaactacg tcgtcctgcc cgagcatccc tggtggaccg ccgagcaccc gtggatcgag
     1201 ggcaagccgc tcgatgacga cttcgtctac gccagcaaca ccaaccactg gtggctcacc
     1261 tccgacgagc tccagcagcg catcgtcgcc tgagcccgtg atcccctacg gccgccagtc
     1321 cgtcgacgac gacgacatcg acgccgtcgt ccgtgtcctc aagggtgact gtctgactcg
     1381 cggtccggcc gtcgaggcct tcgagcacgc cctggccgag cgggtcgagg cccgccatgc
     1441 ggtcgccttc tccagcggca ccgccgccct ccacggtgcc gcggccgtcg ccgggctcgg
     1501 ccccggcgac cgggtcgcca cctcgtctct ctccttcgcc gccagcgcgg cctgtgccgc
     1561 ctacgtcggg gccacccccg tctacgtgga catcgaccct acgaccatga acttcgaccc
     1621 cggagcggtg cccgacggca tcgccgccct cgtcgctgtg cactacgccg gcctcccggt
     1681 cgacctcgcc gcgttgaacc accgtccccc ggtcgtcatc gaggacgcgg cgcaggccct
     1741 gggcgcccgc accccggacg gtcccgtcgg caactgcgcc cgcagcgaca tggcctgctt
     1801 ctcgttccac ccggtgaagt cgatcaccac gggcgagggc ggggcggtga cgaccaacga
     1861 cgacgacctg gccgatcgcc tccgggcctt tcgccaccac ggcctccggc cggtgcccga
     1921 agagggcccc tgggccaccc ggatcgacga gatcggcttc aactaccggc tcaccgacct
     1981 gcaggccgcc ctggggacca gccagctcca caagctgaac cggttcgtcg cccgccggga
     2041 ggagctggcc cgccgctacg acgacgctct ggctgacctc gatgtcgtgt gccccgccac
     2101 ggccccggcc ggcaccgtcc atgcccgtca cctctacccg atcctcgtcg agaaccgggc
     2161 gaacgtggtg gccgagctgc accgccgcgc cattggggtg caggtccacc acgtaccgat
     2221 ccaccaccac ccggcctacg ccgcgggaac cacccccgac gacctcccgg tcaccgaggc
     2281 ggtatacgcg cacgtcctct cgctccccct cttctatgac ctgaccgtgg acgagcagga
     2341 caccgtcgtc accgccctcc ggaggatcct ctcgtgaccg cggcggccgt catgcagccg
     2401 acctaccttc cgtggctcgg gtatctcgac ctgatggacc aggtcgacac cttcgtgctg
     2461 ct
//
LOCUS       DASTFV010000335         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1246666, whole genome shotgun sequence.
ACCESSION   DASTFV010000335 DASTFV010000000
VERSION     DASTFV010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246666"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VFP02_06255"
     CDS             complement(<1..305)
                     /locus_tag="VFP02_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9542662.1"
                     /translation="MALALESADGSRRAEILDTAAAVFAQNGVRTSLKDIADACGILP
                     GSLYHHFDSKDAIVAELVERYETEIDELASGAEADPRPPGSRSGRDPVLALATSIFR"
     gene            complement(506..1744)
                     /locus_tag="VFP02_06260"
     CDS             complement(506..1744)
                     /locus_tag="VFP02_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9542663.1"
                     /translation="MLRYGIVGAGFVSAFHLRALESVRGVEVAGITSRTPPVELAESA
                     RARGLGEATVFDSVTQMLPHVDVVAVFAPNDARLAVMEDVAVGGAHLRGLIIEKPLGR
                     NLAEARRVVELANGLGVPTAYFENQIHMKAITNARAQLEATARAMGPLVLARSGEEHA
                     GPHNAWFWDPTRQGGGVLSDMGCHSIAVGWYLLTPPGKAVRHLVPETVSAEVGLLKWG
                     QPRWRAELLDRYGVDYTKTPAEDFATGIVTYRDPDTDHRSKAQFTVSWMYDKQGLRLL
                     MDAIGPGYALEVNTLRSPLELFIGDDAAASVADAETALEKATASRGLLAVQPNEPDLY
                     GYVDEHIDALAAFERGASALLDLDYGLEIVRLTMACYLSAEEGRVVDLTDPATLERLD
                     DYIPLIQQGHGAEVLSMPAW"
     gene            complement(1754..>2137)
                     /locus_tag="VFP02_06265"
     CDS             complement(1754..>2137)
                     /locus_tag="VFP02_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542664.1"
                     /translation="LVASFDVAATRHRNIEVYGTEGTLSVPDPNSFDGPVTVRRLGDA
                     EWVSIELRPATISQQRGIGVAEMAWATSTGRPHRASGDLALHAVDVMASAVTAAHERQ
                     TIDLTTTCTKPPLLAEGLPPNTFDD"
BASE COUNT          312 a          729 c          774 g          322 t
ORIGIN      
        1 caccggaaga tcgaggttgc gagcgcgagg acggggtcgc gtccgctccg cgaccccggc
       61 gggcgcggat ccgcctctgc gccactcgcg agctcgtcga tctcggtctc gtatcgctcg
      121 accaactcgg cgacgatggc gtccttcgag tcgaagtggt ggtagaggct cccgggcagg
      181 atgccgcagg cgtcggcgat gtccttgagc gaagtccgaa ccccgttctg agcgaacacc
      241 gctgctgcgg tgtcgaggat ctcggctcga cgtgagccgt cggccgactc cagcgccagc
      301 gccacgttgg tcacgagacc ccgaggaccg gcctggccgg atcgatcacg cgcacaccca
      361 tcggacattt tctatctcag gccgccaggg gagctcactc ctcaccgaag cagtcgacga
      421 ggaaggcggt tcccgacgac gaggccggag tcactcgtct ctgacacggc ggcccgctca
      481 gtcctcccgc agcccggacc ggcgctcacc aggcgggcat cgacagcacc tcggcgccgt
      541 gtccctgctg gatcaggggg atgtagtcgt cgagtcgttc gagggtcgcg gggtcggtca
      601 ggtcgacgac ccggccctcc tcggcagaga ggtagcaggc catggtcagc cggacgatct
      661 ccaggccgta gtcgaggtcg agcagggccg acgccccccg ctcgaaggcg gcgagggcgt
      721 cgatgtgctc gtcgacgtag ccgtacaggt ccggttcgtt gggctggacg gccagcaggc
      781 cccgcgaggc cgtggccttc tccagcgcgg tctcggcgtc ggccaccgag gccgccgcgt
      841 cgtcgccgat gaacagctcg agcggcgagc gcagggtgtt cacctcgagg gcgtagcccg
      901 ggccgatggc gtccatcagc aggcgcaggc cctgcttgtc gtacatccac gagacggtga
      961 actgggcctt ggagcggtgg tcggtgtcgg ggtcgcggta ggtcacgatc ccggtggcga
     1021 agtcctcggc cggcgtcttc gtgtagtcga cgccgtaacg gtcgaggagc tcggcccgcc
     1081 accggggctg gccccacttg agtaggccga cctcggccga aaccgtctcg ggcaccaggt
     1141 gccggaccgc tttccccggc ggggtgagga ggtaccagcc cacagcgatg ctgtgacacc
     1201 ccatgtcgga cagcacgcca ccgccttggc gggtcggatc ccagaaccac gcgttgtggg
     1261 ggccggcgtg ctcctcgccc gagcgggcca ggaccagcgg acccatggcc cgggcggtgg
     1321 cctcgagctg ggcccgggcg ttcgtgatcg ccttcatgtg gatctggttc tcgaagtagg
     1381 cggtgggtac gcccaggccg ttcgccagct cgacgacgcg acgggcctcg gccaggttgc
     1441 gaccgagggg cttctcgatg atgagccccc ggagatgggc accgcccacc gctacatcct
     1501 ccatgaccgc cagtcgggcg tcgttggggg cgaagaccgc caccacgtcc acatgcggga
     1561 gcatctgggt gaccgagtcg aagaccgtgg cctcgccgag gccgcgggcc cgggcgctct
     1621 cggccagctc gaccggtggc gtgcgggagg tgatcccggc cacctcgaca ccgcggacgc
     1681 tctccagcgc ccggaggtgg aaggccgaca cgaagccggc gccgacgatg ccgtagcgga
     1741 gcatgcagga gtcctagtcg tcgaaggtgt tcggcgggag cccctcggcc agcagcggag
     1801 gcttcgtgca ggtcgtggtg aggtcgatgg tttgccgctc gtgtgctgcg gtgacggcac
     1861 tggccatcac atccaccgcg tggagggcga ggtcgcccga cgcccggtgg ggtcgcccgg
     1921 tactcgtcgc ccaggccatc tcggccacgc ctatcccccg ctgctgcgag atcgtggccg
     1981 gccgaagctc gatcgacacc cactcggcgt cgccgagccg acgcaccgtc accgggccgt
     2041 cgaaggagtt cgggtccggc accgacaggg tgccctcggt gccgtagacc tcgatgttcc
     2101 ggtggcgggt ggcggcgacg tcgaagctgg ccaccag
//
LOCUS       DASTFV010000336         3859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1247136, whole genome shotgun sequence.
ACCESSION   DASTFV010000336 DASTFV010000000
VERSION     DASTFV010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247136"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="VFP02_06270"
     CDS             complement(<1..1361)
                     /locus_tag="VFP02_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET9542665.1"
                     /translation="MSTMEIVALAGSVVLLLFSAVLAVAETAFTHLGRARAEAIDNAR
                     SGKTKDKNRDDDGNGNSADDTNPAHAGVLVGLLARRGQVLNPVLFLVLVCHLSIATIV
                     AAVAYEHWGRSAVLVALGIELVVLYVAAEAAPKTWALQNTDVAAVRVAPLVRALAALA
                     PLRWILRLLIGLANLLVPGKARKEGPSVSEDELLALAGVAAEASVIEASERALIESII
                     EFGDTIAREVMVPRTEMVTVGDDFRVDAAIEVAIAHGFSRLPAYGQGGIDDITGIVYA
                     KDLMRAERDGHSEEPIRDLLRPARVVPETKRVAELLREMQSEQFHIAIVIDEYGGTAG
                     LVTLEDVIEELVGEIQDEFDVEEPLVEPASGGGIRVHARMAVDEVNALLDGELPEEGD
                     WDSVGGLVYHLLGHVPAEGESVEVTGYRLSAEKVQGRRIGRVRVTPRAESVGPADDEG
                     GGG"
     gene            complement(1402..1845)
                     /gene="ybeY"
                     /locus_tag="VFP02_06275"
     CDS             complement(1402..1845)
                     /gene="ybeY"
                     /locus_tag="VFP02_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET9542666.1"
                     /translation="MSAVAAPVVVARDDQAEEPVDIARWADLATAVLAAEGEAGELNL
                     LFVDEAAMAVLNQDHMGHEGPTDVLSFPIDGPGAGPDGLLGDVVVCPAVARRNAPDHA
                     GTFEDEVALLVVHGVLHVLGHDHAEADEAVVMRQREVTHLERFGR"
     gene            complement(1842..2786)
                     /locus_tag="VFP02_06280"
     CDS             complement(1842..2786)
                     /locus_tag="VFP02_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET9542667.1"
                     /translation="MKINVPGNHLMVGLLGQRDELLRLIEAAFSDTEIRVRGNEITVE
                     GEETEAVARLFEELVILVQRGHALEPAEVVRTIEMIRADERPSEVLTAEVLRGFKGRT
                     VRPKSSGQKRYVDAIADNIITIGIGPAGTGKSWLAVATAVQALQAKEVERIILTRPAV
                     EAGERLGFLPGDLMAKIDPYLRPLYDALFDMVGAEGAERLMERGTVEVAPLAFMRGRT
                     LNGSFIILDEAQNTSPEQMKMFLTRIGFGSKAVITGDTTQVDVQHGRSGLEGLEQILT
                     DIDGLAFVRLGSKDVVRHRIVQDIVDAYDKASLNRSPE"
     gene            2867..3385
                     /locus_tag="VFP02_06285"
     CDS             2867..3385
                     /locus_tag="VFP02_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542668.1"
                     /translation="MTDSSDPEALARAARLAQAADARSRRSDWDQLNAEEATLGSTLE
                     RLATAGSAVRLETAGGPHHGSVTELGPDHVRLVDHGRWRYLRLSTVLSVEAAGAHDGP
                     GPDRVARTFVEALSRLVGETVTLVLANGAAPTGTLTAVGSAIVTLARDGPAGPLYASS
                     SAVAAALGEGSG"
     gene            complement(3334..3582)
                     /locus_tag="VFP02_06290"
     CDS             complement(3334..3582)
                     /locus_tag="VFP02_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9542669.1"
                     /translation="MAPEIEWLSTAEAAGRLGITPRTLYRFLDEGQLPGYRFGRVIRL
                     QATDVEAFIEHSRIEPGTLEHLYPDPSPRAAATADDDA"
BASE COUNT          531 a         1377 c         1381 g          570 t
ORIGIN      
        1 atgccgccgc ccccctcgtc gtcggccggg ccaaccgact cggcccgcgg ggtcacccgg
       61 acccggccga tgcggcggcc ctggaccttc tcggccgaga ggcggtagcc cgtgacctcg
      121 accgactccc cctcggccgg gacgtggccg agcaggtggt agacgaggcc gccgacgctg
      181 tcccagtcgc cttcctcggg cagctccccg tccagcaggg cattgacctc gtcgaccgcc
      241 atccgggcat ggacccggat cccgccaccg ctggcgggct ccacgagcgg ctcctcgacg
      301 tcgaactcgt cctggatctc gcccaccagc tcctcgatga cgtcctccag cgtgaccagg
      361 ccggccgtcc cgccgtactc gtcgatgacg atggcgatgt ggaactgctc ggactgcatc
      421 tcccgcagca gctcggccac ccgtttggtc tcgggcacca cccgggccgg gcgcagcagg
      481 tctcggatcg gctcctcgga gtggccgtcg cgctcggccc gcatcaggtc cttggcgtag
      541 acgatgccgg tgatgtcgtc gatcccgccc tggccgtagg ccgggagccg gctgaagccg
      601 tgggcgatgg ccacctcgat ggcggcgtcg acccggaagt cgtccccgac ggtgaccatc
      661 tcggtgcgcg ggaccatgac ctcacgggcg atggtgtcgc cgaactcgat gatcgactcg
      721 atgagggccc gctcgctggc ctcgatgacc gacgcctcgg ccgccacccc ggccagcgcc
      781 agcagttcgt cctcggagac cgacgggccc tccttgcggg ccttgcccgg tacgaggagg
      841 ttggccaagc cgatgagcag gcgcaggatc caacgcagcg gcgccagcgc ggccagggcc
      901 cggacgaggg gcgccacccg gaccgcggcc acgtcggtgt tctgcagggc ccaggtcttg
      961 ggggcggcct cggccgcgac gtagagcacg accagttcga tgcccaacgc caccaggacg
     1021 gctgaccggc cccagtgctc gtaggccacg gccgccacga tcgtggcaat ggacaggtgg
     1081 cacaccagga ccaggaagag caccgggttg agcacctggc cccggcgggc caggagcccg
     1141 accagaaccc cggcatgggc ggggttcgtg tcgtcggcgc tgttcccgtt gccgtcgtcg
     1201 tcccggttct tgtccttggt cttgcccgaa cgggcgttgt cgatggcctc ggcccgggcc
     1261 cggccgaggt gggtgaacgc cgtttcggcc acggccagca cggcggagaa caggagcagc
     1321 accaccgacc cggccagggc cacgatctcc atcgtgctca tgccggcggt gttccgggac
     1381 tcggggggtc cccgtcccgg ctcatcgacc gaaccgttcc aggtgggtga cctcgcgctg
     1441 gcgcatcacc acggcctcgt cggcctcggc gtggtcgtgg cccaggacgt ggaggacgcc
     1501 gtggacgacg agcagtgcca cttcgtcctc gaacgtcccg gcgtggtcgg gggcgttgcg
     1561 acgggccacc gcgggacaga cgacgacgtc accgagcagg ccgtcggggc cggcaccggg
     1621 gccgtcgatg gggaaggaca ggacgtcggt cggtccttcg tggcccatgt ggtcctggtt
     1681 gagcacggcc atggccgcct cgtccacgaa gagcaggttc agctcccctg cctcgccctc
     1741 cgccgcgagc accgcggtgg ccaggtcggc ccagcgggcg atgtcgacgg gctcctcagc
     1801 ctggtcgtcg cgcgccacga ccaccggtgc cgccacggcg ctcactcggg cgaccggttc
     1861 agggacgcct tgtcgtaggc gtccacgatg tcctgcacga tgcggtgccg gaccacgtcc
     1921 ttggagccga ggcgcacgaa ggccaggccg tcgatgtcgg tgaggatctg ctccaggccc
     1981 tcgagcccgg agcgcccgtg ctgcacgtcg acctgggtcg tgtcaccggt gatgacggcc
     2041 ttcgagccga agccgatgcg ggtcaggaac atcttcatct gctcgggcga ggtgttctgg
     2101 gcctcgtcca ggatgatgaa gctgccgttg agcgtgcgcc cccgcatgaa ggcgagcggc
     2161 gccacctcga ccgtgccccg ctccatcagg cgctcggccc cctcggcccc gaccatgtcg
     2221 aacagggcgt cgtagagggg gcgcaggtag gggtcgatct tggccatgag atcgccgggc
     2281 aggaagccca ggcgctcgcc cgcctcgacc gcgggccggg tcaggatgat ccgctcgacc
     2341 tccttggcct gcagcgcctg caccgcggtg gccacggcca gccaggactt gccggtgccc
     2401 gccgggccga tcccgatggt gatgatgttg tcggcgatgg cgtccacgta gcgcttctgt
     2461 ccgctggact tggggcggac ggtgcggccc ttgaagcccc gcagcacttc ggcggtgagg
     2521 acctccgacg gccgctcgtc ggcccggatc atctcgatgg tgcgcaccac ttcggccggc
     2581 tcgagggcgt gaccccgctg cacgaggatg accagctcct cgaagagccg ggccacggcc
     2641 tcggtctcct cgccctcgac ggtgatctcg ttgccccgga cccggatctc ggtgtcggag
     2701 aacgcggcct cgatgaggcg cagcagctca tcgcgctggc cgaggaggcc gaccatcagg
     2761 tggttgccgg ggacgttgat cttcacgggg gacgaaggca ttcgcagtcc gggcgagcgt
     2821 accgccccct gacgcggcgc cccggggaga tctacgttgc cgctcggtga ctgactcgtc
     2881 cgaccccgag gcgctggccc gcgccgcccg cctggcccag gcggccgacg cccgttcccg
     2941 ccggagcgac tgggaccagc tgaacgccga ggaggcgacg ctggggtcga ccctcgagcg
     3001 cctggcgacc gccggctcgg cggtgcgcct ggagacggcg ggcggtcccc accacggctc
     3061 ggtgaccgag ctgggcccgg accacgtccg ccttgtcgac cacggccgat ggcgctacct
     3121 ccggctctcg acggtgctga gcgtggaggc ggcgggggcc cacgacggtc ccgggcccga
     3181 tcgggtggcc cggaccttcg tcgaggcgct gtcccgcctc gtgggcgaga ccgtcaccct
     3241 ggtgctggcc aacggggcgg cacccacggg cacgctgacg gcggtgggtt ccgcgatcgt
     3301 caccctggcg agggacgggc cggccgggcc gctctacgcg tcgtcgtcag cggtcgccgc
     3361 cgccctcggc gaggggtcgg gatagaggtg ctcgagcgtg cccggctcga tccggctgtg
     3421 ctcgatgaac gcctcgacgt cggtcgcctg gaggcggatg acccgcccga agcggtagcc
     3481 cgggagctgg ccctcgtcga ggaaccggta gagcgtgcgg ggggtgatgc cgaggcgacc
     3541 cgccgcctcg gcggtgctca gccactcgat ctcgggtgcc atggagacga acggtagccg
     3601 gagggtgtga cgagaacgcg gatacccccg agggcaaccg ggaggagtcg agaaccatcg
     3661 ggacacgacg gtgaggtgac gccattcaga caacacgaga atgcttgaaa agtacgatct
     3721 aagtacgtaa gctgcctttc tatggcagga catgagtttt ccctcccttc cctgctcgcc
     3781 cgtcccgccg tcaccctgcg ggccgccctc ggtggcctgc tggtgaccgt cgccctgctc
     3841 ggcgtgagcc aggcctacg
//
LOCUS       DASTFV010000337         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1252354, whole genome shotgun sequence.
ACCESSION   DASTFV010000337 DASTFV010000000
VERSION     DASTFV010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252354"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFP02_06295"
     CDS             <1..278
                     /locus_tag="VFP02_06295"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of succinate and
                     diaminoheptanedioate from succinyldiaminoheptanedioate;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HET9542670.1"
                     /translation="EGDTIELVDVAPGAAPSLDHPLLLALVERSGLGVRAKLGWTDVA
                     RFAARGVPAVNLGAGDSALAHNRNERVEGDRLDVSYRALHDLITTGP"
     gene            complement(296..952)
                     /gene="lexA"
                     /locus_tag="VFP02_06300"
     CDS             complement(296..952)
                     /gene="lexA"
                     /locus_tag="VFP02_06300"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET9542671.1"
                     /translation="MPAAKYTPKKKELTDRQRQILDFIEAETTERGYPPSVREIGQAV
                     GLTSPSTVHSHLATLQRRGYLHRDPTKPRAIEVRYDPNSGAGGHERRPVRHVPLVGDV
                     AAGTDVLAQENVEEVYPLPADLTGDGDLFMLRVRGDSMIDAGIFDGDFVVTRAQTTAE
                     RGEIVVAGIPGDEATIKTYNRRDGQIVLEPANEHLSEMVFRDDEVVVYGKVVTVMRRL
                     "
     gene            1122..1514
                     /locus_tag="VFP02_06305"
     CDS             1122..1514
                     /locus_tag="VFP02_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET9542672.1"
                     /translation="MALALVDTHPRPALRLLQGGLDAGPAPAVYRRRRMVALILAVAF
                     VVVAMTGLRAVVLSTGTFDGRPLSAADGSTPVPAGSETLLVQPGQTLWTIARDLQPSG
                     DVRPLVDELAALNGGATLEAGQNLVLPG"
     gene            1529..1993
                     /locus_tag="VFP02_06310"
     CDS             1529..1993
                     /locus_tag="VFP02_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9542673.1"
                     /translation="MHRWEEFAAQAPALAEAGAALLYQHGPGLAYLATVRRDGAPRLH
                     PVCPVVTLGGLWIFVGNQSPKVADLRRDGRYALHAFPATERDDEFSCAGRATVEEDAA
                     VEAAVLEVYLAQGTITQDHTLFEFRVDRALHAAYGPRPSWPPTYARWSAAAD"
BASE COUNT          314 a          862 c          846 g          361 t
ORIGIN      
        1 aggagggcga cacgatcgag ctggtcgacg tggcgccggg cgcagccccg tcgctggacc
       61 atcccctcct gctcgcactg gtcgaacgca gcggcctcgg ggtccgggcc aagttgggct
      121 ggaccgacgt ggcccgcttc gccgcccggg gcgtgccggc ggtgaacctg ggggcaggcg
      181 actccgcgct ggcccacaac aggaacgagc gggtcgaggg cgatcgcctg gatgtcagct
      241 accgggccct gcacgacctg atcaccaccg gcccctgagg ccgcgacgcg tcgcctcaga
      301 ggcggcgcat caccgtcacg accttgccgt acacgacgac ctcgtcgtcg cggaacacca
      361 tctccgagag gtgctcgttg gcgggctcca gcacgatctg gccgtcgcgc cggttgtagg
      421 tcttgatggt ggcctcgtcg cccgggatgc cggcgacgac gatctcgccc cgctcggcgg
      481 tggtctgggc ccgggtgacg acgaagtcgc cgtcgaagat gccggcgtcg atcatggagt
      541 ccccacggac ccgcagcatg aacaggtcgc cgtccccggt gaggtcggcg ggcagcgggt
      601 agacctcctc gacgttctcc tgggccagca catcggtgcc ggcggcgacg tcgccgacca
      661 gcgggacgtg acgcaccggg cgccgctcgt gaccgcccgc acccgagttc gggtcgtagc
      721 ggacctcgat ggcccggggc ttggtcgggt cccggtgcag gtagccccgg cgctgcagcg
      781 tggccaggtg cgagtgcacg gtggagggcg aggtgaggcc cacggcctgg ccgatctccc
      841 gcaccgacgg cgggtagccc cgctcggtgg tctcggcctc gatgaagtcg aggatctggc
      901 gctgacggtc ggtcagctct ttcttcttgg gcgtgtactt ggctgcaggc acgggaggct
      961 cctggagggg gcaggcgacg aacaggtgtt tgtgacggta gcaccccgac aggggtgtga
     1021 caaacatttg ttcgtcgggc cttgacagcg aacagccgtt cgtggttggg tgcaggagcg
     1081 aacaatcgtt cgtgtcacac cccccgagga gaatctcgtc catggctctc gcactggttg
     1141 acacccaccc tcgaccggcc ctccggctgc tccagggtgg gctcgacgcg ggacctgcgc
     1201 cggcggtgta ccggcgtcgt cggatggtgg ccctgatcct ggccgtggcg ttcgtggtgg
     1261 tcgccatgac cgggctccgg gccgtcgtcc tgtcgacggg gaccttcgac ggccgcccac
     1321 tcagcgccgc cgacggttcc accccggtgc ccgccggtag cgagaccctc ctggtccagc
     1381 cgggccagac cctctggacc atcgcccgcg acctgcagcc ctcgggcgac gtccgtcccc
     1441 tggtggacga gctggccgcg ctcaacggtg gggcgacgct cgaggccggg cagaacctgg
     1501 ttctgcccgg ctgacgagcc gccctgccgt gcaccgctgg gaggagttcg ccgcgcaagc
     1561 ccctgcgctg gccgaggccg gcgccgccct cctgtaccag cacggtcccg ggctggcgta
     1621 cctggccacc gtccgtcgcg acggcgcgcc gcgcctgcat ccggtgtgcc cggtcgtgac
     1681 cctcggcggg ctgtggatct tcgtcggcaa ccagagcccc aaggtggcgg acctccggcg
     1741 cgacggccgc tacgccctcc acgccttccc cgccaccgag cgtgacgatg agttctcctg
     1801 cgcgggtcgg gccacggtcg aggaagacgc ggccgtcgag gccgccgtgc tcgaggtcta
     1861 cctggcccag ggcacgatca cccaggacca caccctgttc gagttccgtg tcgaccgcgc
     1921 cctccacgcc gcctacggcc cccgcccctc ctggcccccg acctacgcgc ggtggtccgc
     1981 cgccgcggac tgaatcgacg ggtcgtcggg cggcggggac cgacaaccgc ggcaaccacg
     2041 ggtcgtccca acttgcgtcg atttgcccac agatctgtgg gtaaatcgac gcaagttggt
     2101 gagggtgacc gcgcggggtg agattccgga ccaccgggcg gccgcgtgcc cccgacagcc
     2161 cgcagcacga ccccacgccc cttcgttgcg tcggtgtcgt tcccccctcg gctgcaacga
     2221 gccgggggcg gggagtaccg ttcccggccg tgcggtgccc ggcgtgttcc tccctcgacg
     2281 acaaggtggt cgacagtcgg acgaccgacg acggcacggc ggtgcgccgg cggcgggagt
     2341 gcctcgagtg cgccacccgc ttcaccacct tcgagcgcct cga
//
LOCUS       DASTFV010000338         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1253462, whole genome shotgun sequence.
ACCESSION   DASTFV010000338 DASTFV010000000
VERSION     DASTFV010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253462"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VFP02_06315"
     CDS             complement(<1..689)
                     /locus_tag="VFP02_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542674.1"
                     /translation="MAVDKFLAVAAGSLLVAAVLVSALRTVVLPQGGQTGITRFVFAV
                     ANRVFTGDTTRRLLPRRLSGLYAPVALVSLPFAWCMLIAVGFSFVFWGLDIGSFSDAW
                     VTSGSSLFTLGFAKPEGGGLIWLTFVEATIGLGVVALLISFLPTLYAAYSAREQGVGM
                     LAPLTGSPASATQLLWRLHAAHSLAGSGIWSSTSAWFVAVEQSHIAFPALSQFPPQQE
                     ERSWVATAGTVL"
     gene            749..2095
                     /locus_tag="VFP02_06320"
     CDS             749..2095
                     /locus_tag="VFP02_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET9542675.1"
                     /translation="MIPIVTPEEMGVIDAAAPEPVEVLIGRAGAAVARAVVDLLGGTY
                     GRRVVVLAGKGNNGNDGRDAARRLRRRGMRVHVIDAADLPEALPACDLVIDAAYGTGF
                     RGEWTPPTVPAPVLAVDIPSGVDGLTGQMPGGALTAARTVTFAALKPGLLLPPGADRA
                     GQVDVADIGLDVSGARAHVVEGADVASWLPRRATAGHKWQAAVRVVAGSPGMTGAAHL
                     TSAAAFRAGAGYVTLSSPGVDHDPMAPIEVVGHPLPAAGWSKDVLGDADRYGALAVGP
                     GLGGDGAEDVRAVVAGWSGPVVVDGDGLTALGESVADVTGGRSGPAVLTPHEGEFARL
                     AGEPVGADRLAVVRSLAAATGAIVLLKGPTTVVAAPDGAVLVTTTGDARLATAGTGDV
                     LTGVIAALLAGGLDPLRAAAAGAFLHGRAGAVGWRHGLVASDLLDQLPIVIDPLQE"
BASE COUNT          283 a          741 c          825 g          270 t
ORIGIN      
        1 aggacggtgc ccgcagtggc cacccacgat cgttcctcct gctggggtgg gaactgggac
       61 agcgcgggga acgcgatgtg gctctgctcg acggcgacga accatgcgga ggtagagctc
      121 cagatccccg atcccgccag cgaatgggcg gcgtggaggc gccagaggag ctgggtggcc
      181 gacgccggcg agccggtgag gggggcgagc atgcccaccc cctgctcccg ggcgctgtag
      241 gcggcgtaga gcgtcgggag gaagctgatc aggagggcca cgacgcccag cccgatggtg
      301 gcctcgacga acgtgagcca gatcaggcca ccgccctcgg gcttggcgaa gccgagggtg
      361 aacaaggacg agccactggt cacccaggcg tcgctgaagc tgccgatgtc gaggccccag
      421 aagacgaagg agaatccgac cgcgatgagc atgcaccagg cgaacggcag ggacaccaac
      481 gccaccggcg cgtagaggcc ggagaggcgg cgggggagaa gtctccgtgt ggtgtcgccg
      541 gtgaacactc ggttggcgac ggcgaacacg aagcgggtga tcccggtctg gccgccctgg
      601 gggagcacga ccgtgcgcag ggcggagacg aggacggcgg cgacgaggag ggaaccagcg
      661 gcgaccgcca agaacttgtc aaccgccacg gcgccagtat ggccgaccgt cggccccctc
      721 ggccggtgtc gtcgggcatc atcacggggt gatcccgatc gtcacccccg aggagatggg
      781 cgtcatcgac gccgccgctc ccgaaccggt cgaggtcctg atcggccggg ctggtgccgc
      841 ggtcgcccgg gccgtcgtcg atcttctggg cgggacctac ggccgccggg tggtcgtgct
      901 ggcggggaag gggaacaacg gcaacgacgg ccgggacgcg gcccgacgcc tgcggcgtcg
      961 cgggatgcgg gtccacgtga tcgacgccgc cgacctcccg gaggcgctgc cggcctgtga
     1021 cctggtgatc gacgccgcct acgggacggg gttccggggg gagtggacgc ctccgaccgt
     1081 gcccgctccc gtgctcgccg tcgacatccc gtccggtgtg gacgggctca ccggccagat
     1141 gccgggcggg gccctgaccg cggcccgcac ggtgaccttc gccgccctca aacccggcct
     1201 gctgctcccg ccgggggccg accgggccgg gcaggtggac gtggccgaca tcggcctcga
     1261 cgtgtctggg gcccgggccc acgtcgtcga gggcgccgat gtggcctcct ggctgccccg
     1321 ccgggccaca gcggggcaca agtggcaggc tgccgtgcgg gtggtggccg gttcgccggg
     1381 catgaccggc gccgcccacc tgacctccgc cgccgccttc cgggccgggg cggggtacgt
     1441 caccctgtcc agccccggtg tcgaccacga ccccatggcc ccgatcgagg tcgtaggcca
     1501 tccgctccct gcggcgggat ggtccaagga tgtgctcggc gatgcggatc ggtacggggc
     1561 gctggcggtc gggcccgggt tggggggcga cggagccgag gacgtccggg cggtagtggc
     1621 gggttggtcc gggcccgtgg tcgtcgacgg cgacgggctc accgcgctgg gcgagtccgt
     1681 ggccgacgtg accggcgggc gatccgggcc cgcggtgctc accccccacg agggcgagtt
     1741 cgcccggctg gcgggggagc cggtcggtgc ggaccgtctc gccgtcgtcc gttccctggc
     1801 cgcggcgacg ggcgcgatcg tcctgctcaa ggggccgacg accgtcgtcg ccgccccgga
     1861 cggcgcggtg ctggtcacga ccaccgggga tgcccgtttg gccaccgccg gcaccggcga
     1921 cgtcctcacc ggggtcatcg ccgccttgct ggccggtggc ctcgatcctc tgcgggcggc
     1981 ggcagccggc gccttcctcc acggccgggc tggagccgta ggctggcggc acggcctggt
     2041 cgccagtgac ctgctcgacc agctcccgat cgtcatcgac ccgctccagg agtgatccat
     2101 gccccgcgac gtccccgcc
//
LOCUS       DASTFV010000339         2265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1258543, whole genome shotgun sequence.
ACCESSION   DASTFV010000339 DASTFV010000000
VERSION     DASTFV010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258543"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2265)
                     /locus_tag="VFP02_06325"
     CDS             complement(<1..>2265)
                     /locus_tag="VFP02_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542676.1"
                     /translation="YVEPGEYDQGQHDAVRDVYYLPGGCQLIRADLFATLGGFDPAQR
                     FYGEDLDLCWRARVAGAQVIVAPAAHARHVADLPARRPDLDEYRLSERHRLRTVLTCY
                     GLGTLVWLAPLLALLSLVDFGAGLVTARAGRARAALGAWTWNLARFSQIIGRRRRLSR
                     LRRTSDRDLRALQAHGLVRVQAFQHRQRPDERRSAVTTVREGVVDFVRSGPSGVSTAV
                     WVVLVFLLLIGSRHLITRSIPAVGELAPFPGGPSVLWNEFFSGWRSAGLGSDAPAPTA
                     FLALSAWGTLFLTFMGLARTVFILGLIPLGGFGAWRLLRETDSVYGRVTALIVYVASP
                     VAYNALADGSLRGLIAYAAAPFVLGGLVRASRLEPFTGEDRGLWRAGLGLGLTTAIGA
                     LFLPLLVVIVLAMALGLGFGIVLTGQGAAAGRVVATGLLGAAVAVLLQFPWSLDLFGA
                     DTPWTAVGGIREGAGALTVPELLRFESGPVGAGMAGFAFLVLAVVPIVVGREWRAAWA
                     LRVWAMALVGWGLLWAGEAGWSPVALPAPEVLLAPAAAAVAMAAGLAAVAFERDVVGK
                     GFSWRQIGLVVGIVALIIGMGPFLASVGNGRWYLPRNDFTVALSFLNAEEEDAPFRVL
                     WIGDPDVLPLSGWRLNEDTAYATSDNGLPDVTELFPGSTTGGTEMLAEALRTATRRET
                     NRLGDLLGPMGIRYVVVPERLAPQPFAREEYPLDPAVSDALSSQLDLVQINVNPAVTL
                     YENAAWIPTRALLPD"
BASE COUNT          386 a          807 c          837 g          235 t
ORIGIN      
        1 gtccggcaag agggcacggg tcgggatcca cgccgcgttc tcgtagaggg tcacggcggg
       61 attgacgttg atctggacca ggtcgagctg gctggacagg gcgtcgctga cggccgggtc
      121 caacgggtac tcctccctgg cgaacggctg gggggcgagg cgctcgggca cgaccacata
      181 ccggatgccc atcgggccga gcaggtcacc gaggcggttg gtctcgcgcc gtgtcgccgt
      241 gcggagggcc tcggccagca tctcggtccc gcccgtcgtg ctgcccggga acagttcggt
      301 gacgtcgggc agcccgttgt cggacgtggc gtacgccgtg tcctcgttga gccgccaacc
      361 cgacaagggc agcacgtcgg ggtcgccgat ccagagcacc cggaacgggg cgtcctcctc
      421 ctcggcgttc aggaagctga gggcgacggt gaagtcgttg cggggcaggt accaccgacc
      481 attgcccacc gacgccagga acggacccat gccgatgatg agggccacga tgccgaccac
      541 caggccgatc tgacgccagc tgaaaccctt gcccacgaca tcacgctcga aagcgacggc
      601 ggcgagcccc gcggccatgg cgacggcggc cgccgccgga gcgagcagga cctcgggtgc
      661 gggcagggcg accggagacc atcccgcctc ccccgcccac agcaggcccc agccgacgag
      721 ggccatcgcc cagacccgta gggcccacgc ggcccgccac tcgcggccca cgacgatcgg
      781 cacgacggcc aggaccagga aggcgaaccc ggccatcccg gcgccgaccg gtcctgactc
      841 gaagcgcagg agctcgggca cggtgagggc gcccgcccct tcccggatcc cgccgacagc
      901 ggtccacggg gtgtcagcgc cgaagaggtc gaggctccag gggaactgca ggagcacggc
      961 gacggccgcg ccgagcagtc cggtggcgac gacccgaccc gcggccgccc cttgaccggt
     1021 gagcacgatg ccgaatccga gccccagcgc catcgccagg acgatcacga ccagcagggg
     1081 caggaacagg gcaccgatcg ccgtggtcag gcccagcccc aggcccgccc gccagaggcc
     1141 acggtcctcc ccggtgaacg gctccaggcg gctcgcccgt accagccccc cgagcacgaa
     1201 gggcgcggcg gcgtaggcga tcaggccgcg gagggagccg tcggccaagg cgttgtaggc
     1261 cacgggcgag gcgacgtaga cgatcagcgc ggtgacccgc ccgtagaccg agtcggtctc
     1321 tcgcaggagc cgccaggccc cgaagccccc gagcgggatc aggcccagga tgaacaccgt
     1381 cctggccagg cccatgaacg tcaggaagag ggtgccccag gcgctgaggg cgaggaacgc
     1441 cgtcggagcg ggggcgtcgg atcccaggcc ggccgaccgc cagcccgaga agaactcgtt
     1501 ccagagcacg ctggggccgc cagggaacgg ggccagttcg cccaccgcgg ggatggagcg
     1561 ggtgatcagg tgacggctcc cgatcaggag gaggaagacg agcaccaccc agaccgcggt
     1621 cgagacacca ctcggcccgg agcgcacgaa gtccacgacg ccctcgcgga ccgtggtcac
     1681 cgccgaccgg cgctcgtccg ggcgttgccg atgctggaag gcctggaccc ggaccaggcc
     1741 gtgggcctgc agtgcccgca ggtcacgatc cgaggtccgc cgcagtcggg acaatcggcg
     1801 gcgccggccg atgatctggg agaaccgggc caggttccag gtccacgccc cgagggcggc
     1861 ccgggcccgg cccgctcggg cggtcaccag gcccgcgccg aagtcgacca aggacagcaa
     1921 ggccagcaga ggggccagcc agaccaaggt gccgaggccg tagcaggtca ggacggtgcg
     1981 cagacggtgc cgctcgctga ggcggtactc gtcgaggtcc ggtcgccggg cgggaaggtc
     2041 ggcaacgtgg cgggcgtggg ccgccggggc cacgatcacc tgcgccccgg cgacacgggc
     2101 gcgccagcac aggtcgagat cctccccgta gaagcgctga gcggggtcga acccgccgag
     2161 cgtggcgaac aggtcggccc ggatgagctg acagccgccg ggcaggtagt agacgtcacg
     2221 gacggcgtcg tgctgtccct ggtcgtactc ccccggctcc acgta
//
LOCUS       DASTFV010000340         3002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1258670, whole genome shotgun sequence.
ACCESSION   DASTFV010000340 DASTFV010000000
VERSION     DASTFV010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258670"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1778
                     /gene="arc"
                     /locus_tag="VFP02_06330"
     CDS             <1..1778
                     /gene="arc"
                     /locus_tag="VFP02_06330"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HET9542677.1"
                     /translation="ADRSRQSRSRRDPDPTPEEAPEPSELQRQIQFLEEETALLRRRL
                     QDSPRQVRVLEERLLETKGQLAQALSQNERLAATLREAKEQIIALKEEVEKLTAPPSG
                     FGVFLGVNDDGTINISSGGRKLRVNVHPDIDPKSLQPGQELMLNEALNVVEACTYEVQ
                     GEVVQLKEMLGVDRALVIGNADEERVVQIGEPLRNRTLRAGDSLLLDVRSGFVLEHLP
                     KPEVEELILEEVPDISYQDIGGLADQIEQIRDAVELPFLHADLFAEHQLKPPKGILLY
                     GPPGCGKTLIAKAVANSLAKKVAEVTGQPSGRSYFLNIKGPELLNKYVGETERQIRLI
                     FQRAKEKSNEGVPVIVFFDEMDSIFRTRGSGVSSDVENTIVPQLLSEIDGVEQLKNVI
                     VIGASNREDMIDPAILRPGRLDVKIKIERPDASAARDIFSKYLLVSLPLAEDVVASAG
                     GDREAALSRIIDEGVKRMYSDADENRFLEVTYANGDKEVLYFKDFNSGAMIENIVSRA
                     KKMAIKRFLGTGEKGIKAADLFAAVTDEFKENEDLPNTTNPDDWARISGKKGERIVYV
                     RTLIEGGNSKDLGRTIEQVNAGQYL"
     gene            1821..>3002
                     /gene="dop"
                     /locus_tag="VFP02_06335"
     CDS             1821..>3002
                     /gene="dop"
                     /locus_tag="VFP02_06335"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: protein motif:HMM:TIGR03688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HET9542678.1"
                     /translation="MAITKVMGIETEYGITVPGAPDSNPVLASSLLVNAFSGRGRKVR
                     WDYEEESPLRDARGFEAAREPETPAEDDLGLANVILTNGARYYVDHAHPEYSSPECLD
                     PRTLVAYDKAGERILAESARRAAELAPGGRRIQVYKNNTDSKGTSYGTHENFLVDRAT
                     PFARIVRDLTPFLVTRQVFAGAGNLVRDHHDGSVAFQIAQRSQFFEVEVGLETTLKRP
                     IVNTRDEPHADPERYRRLHVIIGDANMNEVATFLKTGSLAIVLNMIEDGVVPGDEWQL
                     ANPVQALRAIAKDPTLRTTVERKDGRRLTACELQWHYLDAAKTYLKDRETSHWQIEVL
                     ERWEAVLAQLEDDPLKAARTVDWATKYQLMQQIADRDGLEWEDAKLALVDLQYHDVHM
                     DK"
BASE COUNT          550 a         1087 c          976 g          389 t
ORIGIN      
        1 acgccgaccg ctcccggcag tctcggtccc gtcgcgaccc cgaccccaca ccagaggagg
       61 cgcccgagcc cagcgagctg cagcggcaga tccagtttct cgaggaggag acggccttgc
      121 tccgccgccg cctgcaggac tcgccacgcc aggtcagggt gctcgaggaa cgcctcctcg
      181 agaccaaggg ccagctcgcc caggcgctgt cccagaacga acgcctggcc gccaccctca
      241 gggaggccaa ggaacagatc atcgccctca aggaggaggt cgagaagctc accgccccgc
      301 catcgggctt cggggtgttc ctgggcgtca acgacgacgg caccatcaac atctcctcgg
      361 gcgggcgcaa gctgcgggtc aacgtccacc ccgacatcga ccccaagtcg ctccagcccg
      421 gccaggagct gatgctcaac gaggccctca acgtggtcga ggcctgcacc tacgaggtcc
      481 agggcgaggt cgtgcagctc aaggagatgc tcggggtcga ccgggccctg gtcatcggca
      541 acgccgacga ggagcgggtg gtgcagatcg gcgagccgct gcgcaaccgc accctgcggg
      601 ccggggactc gctgctgctg gacgtccgct ccgggttcgt gctcgagcac ctgcccaagc
      661 cggaggtcga ggagctcatc ctggaggagg tgcccgacat ctcctaccag gacatcggcg
      721 gcctggccga ccagatcgag cagatccgcg acgccgtcga gctgccgttc ctgcacgccg
      781 acctgttcgc cgagcaccag ctcaagccgc ccaagggcat cctgctgtac ggccccccgg
      841 gctgcggcaa gaccctgatc gccaaggcgg tggccaactc gctggccaag aaggtcgccg
      901 aggtgaccgg ccagccctcg gggcgcagct acttcctcaa catcaagggc cccgagctgc
      961 tcaacaagta cgtcggggag accgagcgcc agatccggct gatcttccag cgggccaagg
     1021 agaagtccaa cgagggcgtg ccggtgatcg tgttcttcga cgagatggac tcgatcttcc
     1081 ggacccgcgg gtcgggggtg tcctccgacg tcgagaacac catcgtgccc cagctcctgt
     1141 ccgagatcga cggggtcgag cagctcaaga acgtgatcgt gatcggcgcc tccaaccgcg
     1201 aggacatgat cgacccggcg atcctgcggc ccggccgcct ggacgtgaag atcaagatcg
     1261 agcggcccga cgcctccgcg gcccgcgaca tcttctccaa gtacctgctg gtctccctgc
     1321 ccctggccga ggacgtggtc gcctcggccg gcggcgaccg cgaggccgcc ctgtcccgga
     1381 tcatcgacga gggcgtcaag cggatgtact ccgacgccga cgagaaccgc ttcctcgagg
     1441 tcacctacgc caacggcgac aaggaggtcc tgtacttcaa ggacttcaac tccggggcga
     1501 tgatcgagaa catcgtcagc cgggccaaga agatggccat caagcggttc ctggggaccg
     1561 gcgagaaggg catcaaggcg gccgacctgt tcgccgccgt gaccgacgag ttcaaggaga
     1621 acgaggacct gcccaacacc accaaccccg acgactgggc gcgcatctcg ggcaagaagg
     1681 gcgagcgcat cgtgtacgtg cggaccctga tcgagggcgg caactccaag gacctcgggc
     1741 gcacgatcga gcaggtcaac gccgggcagt acctgtaggc ttcggcctgg gggagcgctc
     1801 cggggagagg gaaatcgcgg atggccatca ccaaggtcat gggcatcgag accgagtatg
     1861 gcatcaccgt gcccggcgcc ccggactcca acccggtgct ggcctccagc ctgctcgtca
     1921 acgccttctc gggccgcggc cgcaaggtcc gctgggacta cgaggaggag tcgccgctgc
     1981 gcgacgcccg cgggttcgag gccgcccgcg agcccgagac cccggccgag gacgacctgg
     2041 gcctggccaa cgtcatcctg accaacggcg cccgctacta cgtcgaccac gcccaccccg
     2101 agtactcctc gcccgagtgc ctggacccgc ggacgctggt cgcctacgac aaggcggggg
     2161 agcggatcct ggccgagtcg gcccggcggg cggccgagct ggccccgggc gggcggcgca
     2221 tccaggtcta caagaacaac accgacagca agggcacctc ctacggcacc cacgagaact
     2281 tcctggtcga cagggcgacc ccgttcgccc ggatcgtccg cgacctgacc ccgttcctgg
     2341 tcacccgcca ggtgttcgcc ggcgcgggca acctggtccg cgaccaccac gacggctcgg
     2401 tcgccttcca gatcgcccag cggtcccagt tcttcgaggt cgaggtgggc ctggagacga
     2461 ccctcaagcg gcccatcgtc aacacccgcg acgagcccca cgccgacccc gagcgctacc
     2521 ggcgcctgca cgtgatcatc ggcgacgcca acatgaacga ggtggccacc ttcctcaaga
     2581 ccgggtcgct ggccatcgtc ctcaacatga tcgaggacgg cgtcgtcccc ggcgacgagt
     2641 ggcagctggc caacccggtc caggcgctgc gggccatcgc caaggacccg accctgcgca
     2701 ccaccgtcga gcgcaaggac ggccgccgcc tgaccgcctg cgagctccag tggcactacc
     2761 tggacgcggc caagacctac ctcaaggacc gcgagaccag ccactggcag atcgaggtcc
     2821 ttgagcgctg ggaggccgtg ctggcccagc tcgaggacga ccccctgaag gcggcccgga
     2881 ccgtcgactg ggcgaccaag taccagctca tgcagcagat cgccgaccgc gacggcctgg
     2941 agtgggagga cgccaagctc gccctggtcg acctccagta ccacgacgtc cacatggaca
     3001 ag
//
LOCUS       DASTFV010000341         2564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1260319, whole genome shotgun sequence.
ACCESSION   DASTFV010000341 DASTFV010000000
VERSION     DASTFV010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260319"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFP02_06340"
     CDS             <1..239
                     /locus_tag="VFP02_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542679.1"
                     /translation="FPADDQGCPTNMTLRSLAIIRPKDMPPVEVILVESPQPNSPYGI
                     KGVGEIGLVPTAGAVAAALHAHDGQWRSQLPMSV"
     gene            239..1273
                     /locus_tag="VFP02_06345"
     CDS             239..1273
                     /locus_tag="VFP02_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9542680.1"
                     /translation="MGPAAVSPGLVCAHHHLYSALARGMPAPPRTPTTFREILELVWW
                     RLDEALDLDTIGWSARLGALEALAVGCTAIVDHHESPNAIDGSLSVIADACAEVGVRV
                     VCAYGVTDRHGADGAGRGLKENRRFLADGGRGMVGLHAAFTCSDDTIAAAADLAVEHG
                     VGVHVHVAEGPDDVEAADRLAPYATDDWLLVHGVHLADDHGLAGTIVHNPRSNMNNAV
                     GYARPTRFANPVALGTDGIGADMLEEFRLAYARLRESDVTVTPDTPWSWLEGSRRIVP
                     EVADDRVTWSVDTVDPWSLAFTTGVQPVEVEIGGEVVWADGRATRVDGDEIHAKAAEA
                     AHRLWARMDP"
     gene            1270..2265
                     /locus_tag="VFP02_06350"
     CDS             1270..2265
                     /locus_tag="VFP02_06350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9542681.1"
                     /translation="MTDRLALYLQDAHPIAEGIEICRYAEAKGFEAVWQAESRLVREA
                     TVPMAAFLSHTTTLKAGSGVVNNWTRNPALLASTFATLDDLAPGRVILGIGAWWDPLA
                     AKVGIDRSRPLRAMRETVEACRALLADETVTYEGEFVHLDGVELDYVHQERRPKDVPI
                     YIGATGMQMMELTGRIADGVLLNYLVSPAYNDRALEHLTRGAEAAGRSVDDLDRPQLV
                     VCSVDDDRQVALDAARLLVTQYLGQQPHIMKASGVPESLLEEIGRVLTWPATHEQVVE
                     ASRLVPDDIVQMICAAGTPDECREKVAEYVARGCTCPVLYPLGPDVKKMIDVFAP"
     gene            2262..>2564
                     /locus_tag="VFP02_06355"
     CDS             2262..>2564
                     /locus_tag="VFP02_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542682.1"
                     /translation="MILRNPLAGPVEPTAPGDAPYAFHRRLPGYAPRPVVDLADPFGL
                     GSVAVKDESDRFGLPSFKMLGASWATYRTLLTLLGHEPEWSTVDELADGLVPLRPLT"
BASE COUNT          359 a          937 c          916 g          352 t
ORIGIN      
        1 acttcccggc ggacgaccag ggctgtccga ccaacatgac gcttcgcagc ctggccatca
       61 tccggcccaa ggacatgccg ccggtcgagg tgatcctggt cgagtcgccc cagcccaact
      121 ccccctatgg catcaagggg gtgggcgaga tcggcctcgt ccccacggcc ggcgcggtgg
      181 cggccgccct ccacgcccac gacggccagt ggcgctcgca gctcccgatg tcggtgtagt
      241 ggggcccgcc gccgtctcgc ccgggctggt ctgcgcccac catcacctgt actcggcgct
      301 ggcccgcggg atgccggcac cgccgaggac gcccacgacg ttccgggaga tcctggagct
      361 ggtctggtgg cggctcgacg aggccctcga cctcgacacg atcggctggt cggcccggct
      421 gggggcgctg gaagcgctgg cggtcgggtg caccgcgatc gtcgaccacc acgagtcgcc
      481 caacgcgatc gacggttcgc tgtcggtgat cgccgacgcc tgcgccgagg tcggggtgcg
      541 ggtggtgtgc gcctacgggg tgaccgaccg acacggagcc gacggggccg gccgggggct
      601 gaaggagaac cgccgcttcc tggccgacgg cggccggggg atggtcgggc ttcacgccgc
      661 cttcacctgc tctgacgaca ccatcgcagc cgcggccgac ctcgccgtcg agcacggggt
      721 gggcgtgcac gtccacgtgg ccgaggggcc cgacgacgtc gaggccgccg accgcctggc
      781 gccgtacgcg accgacgact ggctgctggt gcacggggtg cacctggccg acgaccacgg
      841 cctggccggg acgatcgtcc acaacccccg gtcgaacatg aacaacgccg tgggctacgc
      901 ccgcccgacg cggttcgcca accccgtcgc gctcggcacc gacgggatcg gcgccgacat
      961 gctggaggag ttccgcctgg cctacgcccg cctccgggag agcgacgtca ccgtcacccc
     1021 cgacacgccg tggtcgtggc tggaagggag ccggcggatc gtgcccgagg tcgcggacga
     1081 ccgggtgacg tggtcggtgg acaccgtgga cccgtggtcg ctggccttca ccacgggggt
     1141 gcagccggtc gaggtcgaga tcggcggcga ggtggtgtgg gccgacggtc gggcgacgag
     1201 ggtggacggt gacgagatcc acgccaaggc ggccgaggcc gcccaccgcc tgtgggccag
     1261 gatggacccg tgaccgaccg gctggcgctc tacctgcagg acgcccaccc catcgccgaa
     1321 ggcatcgaga tctgccgcta cgccgaggcg aagggcttcg aggcggtgtg gcaggccgag
     1381 agccgcctcg tgcgggaggc gaccgtcccg atggcggcct ttctctccca caccacgacc
     1441 ctcaaggccg ggtccggcgt ggtcaacaac tggacccgga accccgccct gctggcgtcg
     1501 accttcgcca ccctcgacga cctcgccccg ggacgggtca tcctcggcat cggggcgtgg
     1561 tgggacccgc tggcggccaa ggtgggcatc gaccgctcgc gtccgctgcg ggccatgcgg
     1621 gagacggtcg aggcctgtcg ggcgctgctg gccgacgaga ccgtgaccta cgagggcgag
     1681 ttcgtgcacc tcgacggcgt ggagctggac tacgtccacc aggagcggcg gcccaaggac
     1741 gtgcccatct acatcggcgc caccgggatg cagatgatgg agctgaccgg gcgcatcgcc
     1801 gacggcgtcc tcctgaacta cctcgtgtcg cccgcctaca acgaccgggc cctggagcac
     1861 ctgacccggg gagccgaggc cgcgggccgg tccgtcgacg acctcgaccg gccccagctc
     1921 gtcgtctgct cggtcgacga cgaccgccag gtcgccctcg acgccgcccg cctgctcgtc
     1981 acccagtacc tgggccagca gccccacatc atgaaggcct ccggcgttcc cgagtcgctg
     2041 ctggaggaga tcggtcgggt cctgacctgg cccgccaccc acgagcaggt ggtcgaggcg
     2101 tcccgtctcg tccccgacga catcgtccag atgatctgcg ccgcgggcac gcccgacgag
     2161 tgcagggaga aggtggccga gtacgtcgct cgcggctgca cgtgtcccgt gctctacccg
     2221 ctcggccccg acgtgaagaa gatgatcgac gtgttcgccc cgtgatcctg cgcaacccgc
     2281 tggcaggacc ggtcgagccg accgcgccgg gcgacgcgcc ctacgcgttc caccggcgcc
     2341 tgccggggta tgcgccgagg ccggtggtcg acctggcgga ccccttcggc ctcggatcgg
     2401 tggcggtgaa ggacgagtcg gaccggttcg ggctgccgtc cttcaagatg ctgggtgcgt
     2461 cgtgggccac ctaccggacc cttctgaccc tcctcggtca cgaacccgaa tggtccaccg
     2521 tcgacgagct ggccgacggc ctggtgccct tgcgcccgct gacc
//
LOCUS       DASTFV010000342         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1262988, whole genome shotgun sequence.
ACCESSION   DASTFV010000342 DASTFV010000000
VERSION     DASTFV010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262988"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..415
                     /locus_tag="VFP02_06360"
     CDS             179..415
                     /locus_tag="VFP02_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HET9542683.1"
                     /translation="MTVRAYVLIQTEVGKAAAVAAEIGALEGIVSADDVTGPYDVIAT
                     AEADSVDDLGKMVVSRVQMIEGITRTLTCPVVNL"
     gene            complement(416..784)
                     /locus_tag="VFP02_06365"
     CDS             complement(416..784)
                     /locus_tag="VFP02_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542684.1"
                     /translation="MSTETLLDVHTVMAWVLVATNALAGGWALAAHWVEPLRGRALWW
                     FTGLAQATIVAQVLIGVTLSSAKDLEAPEFHEFYGFVALIAAALIFSYRPQVKAYTYL
                     LYGLGGLFLMGLGLRAIYLA"
     gene            828..2006
                     /locus_tag="VFP02_06370"
     CDS             828..2006
                     /locus_tag="VFP02_06370"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:NF005734.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HET9542685.1"
                     /translation="MPIAEGATGVLPSQHLAAALADGWIGGEVDEASIQPASVDLHLG
                     PVAYRIRCSFLPGTETVERRVKAYVVDELDLTGEGAVLETQRPYLIPLQETLDLPDHI
                     RARTNPKSSTGRLDVFTRVITDGGKAFDEIAAGYTGTLWLEVVPLSFTVRVREGLALN
                     QIRLIAGDRVALTDDEVRARHAEQGLLFRADRAVAPEDFRIASGLFLGLDLEGDEVGR
                     VGHRAKPHTALLDMGRISAYQAEDYWDPVRREEGDRIVLAPGAFYLLLSDEAVVIPPD
                     LAAEMTAYDPTAGELRTHYAGFFDPGFGYDPTGLLRGSRAALEVRAHDVPFMVEHRQP
                     VCKLTFEQMLEPPERLYGAGIGSNYQGQVETLGKHFIRPTAHPSLHLPGSARPAPPIA
                     "
     gene            complement(2068..>2325)
                     /locus_tag="VFP02_06375"
     CDS             complement(2068..>2325)
                     /locus_tag="VFP02_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542686.1"
                     /translation="REHGVEVTELVDHGFVQSVYFTDPNGIALEASYWVTEPTGRPAD
                     YVDGEMFGDTDPVPAVRELMTTGELASIPSTRLVDEAADPA"
BASE COUNT          345 a          869 c          792 g          319 t
ORIGIN      
        1 aagccccgca aggtggcggt cggctccggc accgtcgagg ccgaggcgat cgaggccgag
       61 gcggcctagt ccgccaccct ttcaggccga ccccttccgg cctgggacgc gaccccggac
      121 ctcagggtcc ggggtcgttc gcgtacgctg ggcccgacca cacgacaggg ggaacgtcat
      181 gacggttcgg gcctacgtgc tcatccagac cgaggtcggc aaggccgccg ccgtggccgc
      241 cgagatcggc gccctcgagg gcatcgtctc cgccgacgac gtgaccggtc cctacgacgt
      301 gatcgccacc gccgaggccg actcggtcga cgacctcggg aagatggtgg tcagccgggt
      361 ccagatgatc gaaggcatca ccaggaccct cacctgccct gtcgtgaacc tgtagctacg
      421 cgaggtagat ggcgcggagg ccgaggccca tgaggaacag gccacccaag ccgtagagca
      481 ggtacgtgta cgccttcacc tgtgggcggt agctgaagat cagggcggcg gcgatcaggg
      541 ccacgaagcc gtagaactcg tggaactccg gcgcctcgag gtccttggct gacgagagcg
      601 tcaccccgat caggacctgc gcgacgatcg tcgcctgggc caggccggtg aaccaccaca
      661 gcgccctgcc ccgcagcggc tcgacccagt gcgcggccag ggcccagcct ccggcgaggg
      721 cgttggtggc gaccaacacc cacgccatca cggtgtggac gtcgagcagg gtctcggtcg
      781 acatgcggcc gccgagggta ctgccggcgg ccattacgct cgggtcgatg cccatcgccg
      841 agggagccac cggggtcctg cccagccagc acctggcggc ggccctcgcc gacggctgga
      901 tcggcggcga ggtcgacgag gccagcatcc agcccgccag cgtcgacctc cacctcggcc
      961 cggtcgccta ccgcatccgg tgcagcttcc tgccggggac cgagaccgtc gagcgtcggg
     1021 tgaaggccta cgtggtcgac gagctggacc tcaccggcga gggcgcggtc ctcgagaccc
     1081 agcggccgta cctcatcccc ctgcaggaga ccctggacct ccccgatcac atccgggccc
     1141 gcaccaaccc caagagctcc accgggcgcc tggacgtgtt cacgcgggtc atcaccgacg
     1201 ggggcaaggc cttcgacgag atcgccgccg gctacaccgg caccctctgg ctggaggtcg
     1261 tccccctctc cttcaccgtc cgggtccgcg agggcctggc cctcaaccag atccgcctca
     1321 tcgccggcga ccgggtggcc ctgaccgacg acgaggtccg ggcccgccac gccgagcagg
     1381 gcctcctgtt ccgcgccgac cgggcggtgg cccccgagga cttccgcatc gcgtcggggc
     1441 tgttcctcgg cctcgacctc gagggtgatg aggtcggccg cgtcggccac cgagccaagc
     1501 cccacaccgc cttgctggac atgggccgga tcagcgccta ccaggccgag gactactggg
     1561 atcccgtccg ccgcgaggag ggcgaccgca tcgtcctcgc ccccggtgcc ttctacctgc
     1621 tgctgtccga cgaggcggtg gtcatcccgc ccgacctggc cgccgagatg acggcctacg
     1681 acccgacggc gggcgagctg cgcacccact acgccggctt cttcgacccg ggcttcggct
     1741 acgaccccac cggcctgctg cgggggtcac gagcggccct cgaggtccgc gcccacgacg
     1801 tcccgttcat ggtcgagcac cgccagcccg tctgcaagct gaccttcgag cagatgctcg
     1861 agccgcccga gcgcctgtac ggcgcgggta tcggctccaa ctaccagggc caggtcgaga
     1921 ccctgggcaa gcacttcatc cgccccaccg cccacccctc gctacatctg ccgggctccg
     1981 cccggccggc gccgccgatc gcgtgagtcg aacgggctgc tctgctccgc tgcgcagcgt
     2041 cgagcccggc tggccccgtt ggccacgtca ggcggggtcg gcggcctcgt cgaccaggcg
     2101 ggtggaaggg atgctggcca gctcgccggt tgtcatgagc tcgcggacgg cggggacggg
     2161 gtcggtgtcg ccgaacatct cgccgtcgac gtagtccgcc ggacggccgg tgggctcggt
     2221 cacccaatag ctggcctcga gggcgatgcc gttggggtcg gtgaagtaga cggactgcac
     2281 gaagccgtgg tcgaccagct cggtcacctc gacgccgtgc tcccg
//
LOCUS       DASTFV010000343         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1263473, whole genome shotgun sequence.
ACCESSION   DASTFV010000343 DASTFV010000000
VERSION     DASTFV010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263473"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFP02_06380"
     CDS             complement(<1..197)
                     /locus_tag="VFP02_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9542687.1"
                     /translation="MRDHDTIYIDGAWVPSTGSGSIEVVNASTEQVMGSIPEGTAEDV
                     DKAVAAAKAAFETWGRTEPEER"
     gene            complement(276..1382)
                     /locus_tag="VFP02_06385"
     CDS             complement(276..1382)
                     /locus_tag="VFP02_06385"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HET9542688.1"
                     /translation="MDGTTLGVEEEFQIIDRATGELVPRSDELVPAARRSLGDRVTAE
                     LNRCQVETATPVCADLGEVRAELIRLRSELDRVAEAIGCGILPLATHPWSSWQDQQVE
                     DDRARFREMEDRYQRVARQQVICGCHIHVGIEDPDRVVEVMTRVRPWIPVLLALAANS
                     PYWNGEDTGYDSYRLEIWERWPTAGLPPALADRAAYDEVVDELREADAIEDATHLYWY
                     LRPSDRYPTLEFRATDVCLDVDAAVCLAGLARALVRTCVADAQAGVPVGTASSSLLGA
                     SLWRAARYGLAEDLVSPSSAAPLPAADVVRELLARVGGALDQAGDREEVTALTEAILR
                     DGNGARRQREALAQRGDPADVVALGRAGLLTPAD"
     gene            complement(1424..1783)
                     /locus_tag="VFP02_06390"
     CDS             complement(1424..1783)
                     /locus_tag="VFP02_06390"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET9542689.1"
                     /translation="MRLFRRLLPFSTAGGAAWFAFRHRELLWDWGTWTAQALPRAVGG
                     NAGDVMKEAWLRARLSGDDRVGAREVDVRVIDGYASLRGEVGPGGRVLATQLARDVPG
                     IVGVSDELRERQAQPTG"
     gene            1929..>2172
                     /locus_tag="VFP02_06395"
     CDS             1929..>2172
                     /locus_tag="VFP02_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542690.1"
                     /translation="MNDRPSRLHLETTREAGLWTLSPVSVLAGVVSAYGVFAIVAAIA
                     GAVLTSIDVNTEFRTNDWTGSGAVAALTSALVLFLAY"
BASE COUNT          301 a          818 c          746 g          307 t
ORIGIN      
        1 cgctcctcgg gctcggtccg gccccaggtc tcgaacgccg ccttcgccgc ggcgacggcc
       61 ttgtcgacgt cctcggcggt gccctcgggg atgctgccca tgacctgctc ggtcgaggcg
      121 ttcaccacct cgatggagcc gctcccggtg gagggcaccc aggccccgtc gatgtagatc
      181 gtgtcgtggt cgcgcacgag tccccctgtg gttcgtctgt ggtcgggctg cgcttcggtg
      241 gacgcttgac ggtactcgcg ccgctgacga tcgcgtcagt cagccggggt gagcagtcca
      301 gcccggccga gggcaacgac atcggcgggg tcgccgcgct gcgccaacgc ctcccgttgg
      361 cgtcgggccc cgttgccgtc ccgcaggatc gcctcggtca gcgccgtgac ctcctcccgg
      421 tcgcccgcct ggtcgagagc ccctcccacc ctggccagca gctccctcac cacgtccgcg
      481 gcagggagcg gcgcggccga gctcggcgac acgaggtcct ccgcgaggcc gtagcgggcc
      541 gcccgccaca gggacgcgcc gagcaacgag gacgaggcgg tcccgaccgg caccccggcc
      601 tgggcgtcgg ccacgcaggt ccggaccaac gcccgggcca ggccggccag gcacacggcc
      661 gcgtcgacgt ccaggcagac gtcggtggcc cggaactcga gggtcggata gcgatcggac
      721 ggtcgcaggt accagtacag gtgggtggcg tcctcgatgg cgtcggcctc ccggagctcg
      781 tccaccacct cgtcgtaggc cgcccgatca gccagggcgg ggggcaggcc ggccgtcggc
      841 caccgttccc agatctcgag gcggtaggag tcgtagccgg tgtcctcacc gttccagtac
      901 ggggagttgg cggcgagggc cagcaggacc gggatccacg gccggacccg cgtcatcacc
      961 tcgacgacgc ggtcgggatc ctcgatcccc acatggatgt ggcacccaca gatcacctgc
     1021 tgacgggcga cccgttggta ccggtcctcc atctcccgga agcgggcccg gtcgtcctcc
     1081 acctgctggt cctgccacga gctccaggga tgggtggcca acggcaggat cccgcacccg
     1141 atcgcttcgg cgacccggtc cagctccgag cggagccgga tcagttcggc ccggacctcg
     1201 ccgaggtcgg cacagaccgg tgtcgccgtc tcgacctggc accggttcag ctccgcggtc
     1261 acgcggtcgc cgagcgagcg tcgagccgcc gggacgagct cgtcggaccg ggggaccagc
     1321 tcgccggtcg cccgatcgat gatctggaac tcctcctcca ccccgagggt ggtgccgtcc
     1381 atcccgcatg tcctctcgtc gagcgccctt ccgggcgcgc cggtcacccg gtgggctggg
     1441 cctggcgctc ccggagctcg tcgctgaccc cgacgatccc cggtacgtcc cgagccagtt
     1501 gcgtggccag gacccgacct ccgggcccga cttctccccg gagcgaggca tagccgtcga
     1561 tcacccgcac gtcgacctcc cgggcaccga cccggtcgtc accactcagg cgggcccgga
     1621 gccacgcctc cttcatgacg tcgccggcgt tgccgccgac ggcccgaggc agggcctgag
     1681 cggtccacgt cccccagtcc cagaggagct cacgatggcg gaaggcgaac cacgcggcgc
     1741 ccccggccgt cgagaagggg aggagtcgtc ggaagagtcg catcgtttcg tcctcctacc
     1801 cacgccgcgc cgaaaaggac cacggacgac ggaatcaccg cgatgccctc tccgacgtct
     1861 cactcggcgc ccagtgggca ggtacgggcc aacgcaatga cgcggacgac gagacctggg
     1921 ggtttccgat gaacgaccga ccgagccgac tgcacctcga aaccacccgt gaggcgggcc
     1981 tgtggacgct gtccccggtc agcgtgctgg ccggggtggt gagcgcctac ggcgtgttcg
     2041 ccatcgtggc cgcgatcgcg ggggcggtgc tgacctcgat cgacgtcaac accgagttcc
     2101 gcaccaacga ctggaccggc agcggggccg tggcggcgct gaccagtgcc ctcgtcctct
     2161 tcctggccta cc
//
LOCUS       DASTFV010000344         2265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1271889, whole genome shotgun sequence.
ACCESSION   DASTFV010000344 DASTFV010000000
VERSION     DASTFV010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271889"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VFP02_06400"
     CDS             <1..398
                     /locus_tag="VFP02_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542691.1"
                     /translation="EADPVRTVTRKGAALAMTAALVVGGLIGWVGAAAFDDDAPQLTS
                     FQGRFPGPGGRFPGGGPGGGFPDGGFHEHGPGGRIPDGGYEGEDGRPTPPWMDDDDQG
                     EDDEQGEDDEQGNRDPGQEEQGQDQGDDG"
     gene            complement(432..1070)
                     /locus_tag="VFP02_06405"
     CDS             complement(432..1070)
                     /locus_tag="VFP02_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542692.1"
                     /translation="MNFVSHMVVAQQATPEATTPLLLGAAAPDLARMARLPVATAGPP
                     GFTAGVATHHRTDAVFHGLDWFRATNRRLVRGLSDRGVRRGPARGAAHVLIELLLDGA
                     VLARAEHAATFARPWAALAEPDDDALAMVADEDRSQWVEFLGLLTDRLEPVSYGDPRY
                     AADRTAGTLARRPRLAMNNDERAVLRGLADDVHEPITRDADDVLRSVVAAVL"
     gene            1193..2137
                     /locus_tag="VFP02_06410"
     CDS             1193..2137
                     /locus_tag="VFP02_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET9542693.1"
                     /translation="MLVLTGATLFGINASVSKVVLAAGIEPARLTALRCTGAALGLLF
                     VLKVLRPTKLRMPRQEWPAIVVLGLSGAAFLQWLYFVALDRLPVGIALLLEFTAPLMV
                     ALYSSLVLRHVVPRQVWLALGLALAGLALVAQVWTDVGLDAVGVAAALGAAGFLATFH
                     LVGKQMVDRHDPLLLSFWVFVVAAVFWAVAQPWWRFDPSVLTESTSLLGRLGAYSVPV
                     WVGLLWVILLGTLAPYALEVAGLRYLTATTTGIVSMVEPVIAATVAWLWLEEVLNGVQ
                     LVGGALVLVGVGLVQVAGSRAAPDAEDAPFVVADVPLN"
BASE COUNT          279 a          747 c          880 g          359 t
ORIGIN      
        1 aggaagcgga cccggtccgc accgtgaccc gtaagggagc ggcgttggcc atgacggcgg
       61 ccttggtggt cggtggcctg atcggctggg tgggggcggc ggcattcgac gacgacgccc
      121 cgcagctcac ctcgttccag ggtcggttcc cagggcccgg cggccgcttc ccgggcggtg
      181 gcccgggtgg aggcttcccg gacggcggct tccacgagca cggtccgggc ggccgcatac
      241 cggatggtgg ctacgagggc gaggacggcc gacccacgcc gccgtggatg gacgacgacg
      301 accagggcga ggacgacgag cagggcgagg acgacgagca gggcaaccgg gacccggggc
      361 aggaggagca aggccaggac cagggagatg atggatagcg tcgtcgtggc gggccggagc
      421 cgcggtcgcc gtcagagaac ggcggccacc acgctccgca gcacatcgtc ggcgtccctg
      481 gtgatcggct cgtggacatc gtcggccagc ccccggagca cagcccgctc gtcgttgttc
      541 atcgccagtc gagggcgccg ggccagggtg ccggccgttc ggtcggcggc gtagcgcggg
      601 tcgccgtagc tcactggctc cagccggtcg gtgagcaggc ccaggaactc gacccactgg
      661 gagcggtctt cgtcggcgac catggccagc gcatcgtcgt cgggttcggc gagagcggcc
      721 caggggcggg cgaaggtggc ggcgtgctcc gcccgggcca ggacggcgcc gtcgaggagc
      781 agctcgatga ggacgtgggc ggcaccccgg gctggcccgc ggcggacccc ccggtcgctg
      841 agaccgcgga ccagccggcg gttggtcgcc cggaaccagt ccaacccgtg gaagacggcg
      901 tcggtgcggt ggtgggtggc gacccccgcg gtgaagccgg gtggtccggc ggtggccacg
      961 ggcaagcggg ccatgcgggc gaggtccggg gccgccgccc ccaggaggag cggggtcgtg
     1021 gcttcggggg tcgcctgctg ggcgacgacc atgtgggaga cgaagttcac ctgcgcggaa
     1081 ggctacggag gtggggtgat ctcacgggaa tcggggccag gtgggttcgt ccccgtagcg
     1141 tcgacgcatc gcggcgcccc tccctgctcc gacgcgccat cggcgcgggt acgtgctggt
     1201 cctgaccggg gcgaccttgt tcggcatcaa cgccagcgtg tcgaaggtcg tgctcgccgc
     1261 cgggatcgag ccggcccggc tgacggcact gcgttgcacc ggagcggcgc tcgggctctt
     1321 gttcgtgctg aaggtgctac ggccgacgaa actgcggatg ccccgccagg agtggcccgc
     1381 catcgtcgtg ttgggcctga gcggggcggc cttcctccag tggctctact tcgtggcgct
     1441 ggaccgactt ccggtgggca ttgccctgct cctggagttc accgccccgt tgatggtggc
     1501 gctctactcc agcctggtcc tgcgccacgt cgtcccgcgc caggtctggc tggctctcgg
     1561 cctggctctg gccgggctcg ctctggtcgc ccaggtgtgg acggatgtcg gcctcgatgc
     1621 cgtcggcgtc gccgccgcgc tgggtgcggc gggattcctg gccacgttcc acctcgtcgg
     1681 caagcagatg gtcgaccggc acgacccgct tctgctctcg ttctgggtct tcgtcgtggc
     1741 cgccgtcttc tgggcggtgg cccagccttg gtggcgcttc gacccgtcgg tgctgacgga
     1801 gtcgacctcc ctcctcggtc gattgggcgc ctactcggtg ccggtgtggg tgggcctgct
     1861 ctgggtgatc ctgctgggca cgctcgcccc ctatgccctg gaggtggccg ggctccggta
     1921 cctcaccgcg acgacgaccg gcatcgtgag catggtggag cccgtcatcg cggccaccgt
     1981 ggcctggctc tggttggaag aggtcctgaa cggggtccaa ctggtcggtg gcgctctggt
     2041 gttggtgggt gtcggtctcg tccaggtcgc cggatcccga gccgcgcccg atgcggagga
     2101 cgccccgttc gtcgtggccg acgtgccgct caactagctc gacgggcggg tcgggaccag
     2161 ctgagcgagt gggtgcggac tgagcagatg aacggccggg cttggtcacc taacgtctgc
     2221 agaggaccgg catccacagg aaggctccgc gtgaccattc cccct
//
LOCUS       DASTFV010000345         2844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1272874, whole genome shotgun sequence.
ACCESSION   DASTFV010000345 DASTFV010000000
VERSION     DASTFV010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272874"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="VFP02_06415"
     CDS             complement(<1..1240)
                     /locus_tag="VFP02_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542694.1"
                     /translation="MPRRWGVLAAISLLAIVGCSGDDNGDDAAPATTAADSSAPDATP
                     APDGVVAAIEDDGGGPDCDPTDPRACLLPFPSNRFTVESPDTDTGLLVALPESGMPVN
                     VDGVVADPTEWNRNDGFSPGTALLTQVPGLDVEASGLPAITDTPDSRAEDSPVVVVDT
                     ETGERQPVWAELDSGAEADDDRLLFIRPGVNYLEGHHYVVGLRDLVSDDGEALEPSAG
                     FLVYRDNLTTDLPVIEDERGAMEQVFTDLGEAGVAREDLYLAWDFTIASERNLSERLL
                     HIRDDAFADLGEAAPEFTVDDVVRDPEEAGVEYVHGTFQVPLYLTADGAAGTRFDNGA
                     DGLPRRNGDFTADFECALPTATEVAVPMALYGHGLLGSRDEVEAGNIADFVREHQVAF
                     CATDWIGMSEDDVGNAASILG"
     gene            1276..2208
                     /locus_tag="VFP02_06420"
     CDS             1276..2208
                     /locus_tag="VFP02_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET9542695.1"
                     /translation="MAGNSNRAIIAALIANLGIAIAKFAGFLITASSSLLAESIHSVA
                     DTGNQALLLLGGRRAKKEATEEHPFGFGRERYFWAFVVAVVLFSLGSVFAIFEGIEKL
                     RHPHELESAEVAIAILLVAIVLEGWSFRTAVVESRPLKGDLSWWQFIRRAKQPELPVV
                     LLEDFGALTGLLIALIAISLTIVTDDGMWDALGTLTIGALLGVIAFVLAHEMKSLLIG
                     ESATKFVRDEIEAAIIDSPATVALIHIRTQHLGPDELLVGAKIDFGATLTSAELAKAI
                     DTVEADIRAAVPAATIIYLEPDIQRLTESESAEA"
     gene            complement(2393..>2844)
                     /locus_tag="VFP02_06425"
     CDS             complement(2393..>2844)
                     /locus_tag="VFP02_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542696.1"
                     /translation="TPAERASAPAKRKEIDMSVATEKITEIQDQVRDLVARIEEPVVH
                     GFQTVATKVEDRLSELHIPDLGEKLPTAEQLTDARDKVGEKLADLREQLTEAVATATE
                     PVRRQFVSEPTPEPAAKTTTKAPARPAAKATTKTGAKATKATKVKAA"
BASE COUNT          413 a          978 c          994 g          459 t
ORIGIN      
        1 gacccaggat cgacgccgcg ttgccgacgt cgtcctcgga catcccgatc cagtcggtgg
       61 cacagaacgc cacctggtgc tcgcgcacga agtcggcgat gttccccgcc tccacctcgt
      121 cccggctgcc gaggaggccg tggccgtaga gcgccatggg gacggccacc tcggtggcgg
      181 tgggcagggc gcactcgaag tcggcggtga agtcgccgtt gcgcctgggc aggccatcgg
      241 caccgttgtc gaaccgagtg ccggcggccc cgtcggcggt caggtagagc ggcacctgga
      301 aggtcccgtg gacgtactcc acgccggcct cctccgggtc ccggacgacg tcgtccaccg
      361 tgaactcggg ggcggcctcg cccaggtcgg cgaaggcgtc gtcccggatg tggagcaggc
      421 gctcggacag gttccgctcg ctggcgatcg tgaagtccca ggccaggtac aggtcctccc
      481 gggccacgcc ggcctcgccc aggtcggtga acacctgctc catggcgccg cgctcgtcct
      541 cgatgactgg caggtcggtg gtcaggttgt cccggtagac caggaagcct gccgacggtt
      601 ccagggcctc gccgtcatcg ctcaccagat cgcgcagacc cacgacgtag tggtggccct
      661 ccaggtagtt gaccccgggc cggatgaaga gcagacggtc gtcgtcggct tcggcgccgg
      721 agtccagctc ggcccagacc ggctgacgtt cgcctgtctc ggtgtcgacg acgacgacgg
      781 gcgagtcctc ggcccgcgaa tccggggtgt cggtgatggc gggaaggccc gacgcttcca
      841 catcgagacc gggcacctgg gtcagcaggg ccgtacccgg gctgaagccg tcgttgcggt
      901 tccactccgt ggggtcggcc acgactccgt cgacgttgac cggcatgccg gactcgggca
      961 gggccaccaa gaggccggtg tcggtgtcgg gcgactcgac ggtgaaccgg ttgctgggga
     1021 agggcaacaa gcaggcgcgg gggtcggtcg ggtcgcagtc cggaccaccg ccgtcgtcct
     1081 cgatggccgc cacgacgccg tcgggcgccg gcgtggcatc cggagccgag gagtcggcgg
     1141 ccgtggtggc cggcgccgcg tcgtccccgt tgtcgtcacc cgaacagccg acgatcgcca
     1201 acagggagat cgcggccagg accccccacc ggcgcggcat agggacgcaa gcgtaggcag
     1261 cggtagcttg cgccgatggc gggcaacagc aaccgggcga tcatcgcagc gctcatcgcc
     1321 aacctcggca tcgccatcgc caagttcgcc ggcttcctca tcacggcctc gtcgtcgctc
     1381 ctggccgaga gcatccattc ggtggccgac accgggaacc aggcgctgct cctcctcggc
     1441 ggtcgtcggg ccaagaagga ggcaaccgag gagcacccgt tcggcttcgg ccgggagcgc
     1501 tacttctggg ccttcgtcgt cgccgtcgtg ctcttcagcc tcggctcggt cttcgccatc
     1561 ttcgagggca tcgagaagct gcgccacccc cacgagctgg aatccgcgga agtggccatc
     1621 gccatccttc tggtcgccat cgtgctcgag ggatggtcgt tccggaccgc ggtggtcgag
     1681 tcccgcccgc tcaagggcga cctctcgtgg tggcagttca tccggcgggc caagcagccc
     1741 gagctccccg tggtcctgct ggaggacttc ggggccctga ccgggctgct catcgccctc
     1801 atcgccatca gcctcacgat cgtcaccgac gacggcatgt gggacgccct gggcaccctg
     1861 accatcggtg cgctgctcgg cgtgatcgcc ttcgtgctgg cccacgagat gaagagcctc
     1921 ctcatcggtg agtcggccac gaagttcgtc cgggacgaga tcgaagcggc catcatcgac
     1981 tcccccgcca cggtggccct catccacatc cgcacccagc acctcggccc cgacgagctc
     2041 ctcgtgggcg ccaagatcga cttcggcgcc accctcacca gcgccgagct ggccaaagcc
     2101 atcgacacgg tggaagccga catccgcgcc gcggtccccg cagccacgat catctacctc
     2161 gaacccgaca tccaacgcct gaccgaaagc gagtctgctg aggcatgagc cagaggccgg
     2221 atgtgaccca ttgacgggtc gctgatttgc gccagtcgac ggctcgcaca gacgcaggtc
     2281 gatgtccccg tcaggggccg caaaaataga aacgaggagc gctcgggggg caagcgctcc
     2341 tcgtctctgt tgcggcggtc cggggggagg accgccgatc aggcggcctg tatcaggcgg
     2401 ccttgacctt ggtggccttg gtggccttgg cgccggtctt ggtggtcgcc ttggcggccg
     2461 gccgggcggg ggccttggtg gtcgtcttgg cggccggctc gggggtcggc tcggagacga
     2521 actggcgacg caccggctca gtcgccgtgg cgacggcctc ggtgagctgc tcgcggaggt
     2581 cggccagctt ctcgccgacc ttgtcgcggg cgtcggtcag ctgctcagcc gtggggagct
     2641 tctcgccgag gtccgggatg tggagctcag agaggcgatc ctcgaccttg gtggcgacgg
     2701 tctggaagcc atggacgaca ggctcctcga tccgggcgac gagatcgcgg acctggtcct
     2761 ggatctcggt gatcttctcg gtggcgaccg acatgtcgat ctctttcctc ttggcggggg
     2821 cactggctcg ctcggctgga gtgc
//
LOCUS       DASTFV010000346         2134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1284725, whole genome shotgun sequence.
ACCESSION   DASTFV010000346 DASTFV010000000
VERSION     DASTFV010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284725"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VFP02_06430"
     CDS             <1..920
                     /locus_tag="VFP02_06430"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9542697.1"
                     /translation="AGWGLERANSVHEAVWNVAFLVGPGIGGLLIATVGAASTLWVTA
                     AGFALSLALTSRIRVPGAGIPQAHERPEGFWTGTLEGLRFIRHEPLLRTTALLSMAIV
                     ALYLPVEGVILPTYFNEQGQPARLGTVLMALSLGGIAGSLAYGKWGTRLRRRTAFVLA
                     MVGVGVPLFGMALLPPFTVMVLFATIAGFLYGPIAPLENYAMQTRTPEELRGRAFGVL
                     TSAAYAAGPVGYLLVGPLVEALGLRPAFILLSAGLVLVTLAAIPARSLHLLDEPPLYA
                     ASPDEDWTPTHSGLPLGEQSVPRLRESAA"
     gene            943..1185
                     /locus_tag="VFP02_06435"
     CDS             943..1185
                     /locus_tag="VFP02_06435"
                     /inference="COORDINATES: protein motif:HMM:NF022397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HET9542698.1"
                     /translation="MDDAEILSRITALVDEEHRLEESHIGEGLSDEEKDRLGEVEVAL
                     DQCWDLLRQRRARRNAGLDPDEAAVRPEGTVEGYRQ"
     gene            complement(1187..1921)
                     /locus_tag="VFP02_06440"
     CDS             complement(1187..1921)
                     /locus_tag="VFP02_06440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET9542699.1"
                     /translation="MAGSRAWFFRRRFLNEAIHRTWDWAQSRGAIHPGTIRSERFGSF
                     GEGSLLGFPVATLFGEPQIHIGMGTLIASWVTLSAGYGPDQVDVPARALVIGDRCLIN
                     IRCGLVAHESIEIGDDVWFGQGVYITDANHGFEDFDVPIGKQLGDHQPVSVGRGSWLG
                     HGSVILPGARIGRQCVVAAGSVVRGEVPDHSVVAGVPAKVIRTLTDGRPAHQEGRLDE
                     FVEPLAKGFGTWSVDGRHPRRRSRRA"
BASE COUNT          290 a          815 c          723 g          306 t
ORIGIN      
        1 gcgcgggctg gggcctggag cgggccaaca gcgtgcacga ggccgtctgg aacgtggcct
       61 tcctcgtcgg tcccggcatc ggcggactgc tcatcgccac ggtcggcgcg gccagcacgc
      121 tctgggtcac cgccgccggt ttcgccctct ccctcgccct gacgtcgagg atccgggtgc
      181 ccggcgccgg gatcccccag gcccacgagc ggccggaggg attctggacc gggacgctcg
      241 agggcctgcg cttcatccgt cacgagccgc tcctgcgcac gacggcgctg ctgagcatgg
      301 ccatcgtcgc cctctacctg cccgtcgagg gggtgatcct gccgacctac ttcaacgagc
      361 agggccagcc ggcgcgcctc ggaaccgtcc tcatggccct gagcctcggc ggcatcgccg
      421 gctccctggc gtacggcaag tggggcacga ggctgcgccg caggacggcc ttcgtcctgg
      481 ccatggtcgg tgtcggggtc ccgctgttcg gcatggctct gctcccgccg ttcacggtca
      541 tggtcctctt cgccacgatc gccgggttcc tgtacgggcc catcgccccg ctggagaact
      601 acgccatgca gacccgcacg cccgaggagc tacgaggtcg ggcgttcggc gtcctcacgt
      661 ctgcggccta cgccgcgggc ccggtgggct acctactggt cggcccactg gtggaggccc
      721 tcggccttcg ccccgccttc atcctgctca gcgccggcct cgtcctggtg accctggccg
      781 ccatccccgc ccgcagcctc cacctgctcg acgagccccc gctgtatgcc gcctcacccg
      841 acgaggactg gaccccgacc cactcgggcc tgcccctcgg cgagcagtcc gtcccgcggt
      901 tgcgggagtc ggccgcttga caccgggtgt cggatggatg ccatggatga cgccgagatc
      961 ctgagccgca tcaccgccct cgtggacgag gagcaccgcc tcgaggagag ccacatcggc
     1021 gaaggcctgt cggacgagga gaaggaccgt ctcggcgagg tcgaggtcgc cctcgaccag
     1081 tgttgggacc tgctccggca acgacgggcg cggcgcaacg ccggcctcga ccccgacgag
     1141 gccgcggtgc gtccggaggg caccgtcgag ggctaccggc agtagctcag gcccggcggg
     1201 accggcgccg gggatggcgc ccgtcaaccg accaggtccc gaagcccttg gccagcggct
     1261 ccacgaactc gtcgagccga ccctcctgat gcgccggtcg gccgtcggtc aaggtgcgga
     1321 tcaccttcgc cgggacgccg gccacgacgc tgtggtccgg cacctcgccc cggaccaccg
     1381 accccgcggc caccacgcac tgccggccga tgcgagcgcc ggggaggatg accgatccgt
     1441 ggcccagcca ggacccccgg cccaccgaca ccggctggtg gtcacccaat tgcttgccga
     1501 tcgggacgtc gaagtcctcg aacccgtggt tggcgtcggt gatgtagacg ccctggccga
     1561 accagacgtc gtcaccgatc tcgatggact cgtgcgccac gagcccgcag cggatgttga
     1621 tcaggcagcg gtccccgatg accaacgccc gcgcgggcac gtcgacctgg tccggtccgt
     1681 agcccgcgga cagtgtgacc cagctggcga tgagcgtgcc catcccgatg tggatctgcg
     1741 gctcgccgaa cagcgtcgcc accgggaagc ccagcaggct gccctcgccg aacgacccga
     1801 agcgctcgga ccggatcgtg ccggggtgga tcgccccgcg cgactgcgcc cagtcccagg
     1861 tccggtggat ggcctcgttg aggaagcggc gccggaagaa ccacgcgcgc gatccggcca
     1921 tcccgagagg ctacgggatg cgcccctcgc cctcttgggc ggcgccccgc tcgatggcct
     1981 ccttgggcct acccggccgg gaccgcctca ctacgatgcg ccgatgacgc cggcgggacc
     2041 gaccttcgac aaccgcatca cccggctgtt gggcgtcgag atccccgtcg tgcaggcacc
     2101 gatgggctgg atcgcccgct cccggctggc ctcc
//
LOCUS       DASTFV010000347         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1285876, whole genome shotgun sequence.
ACCESSION   DASTFV010000347 DASTFV010000000
VERSION     DASTFV010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285876"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VFP02_06445"
     CDS             <1..624
                     /locus_tag="VFP02_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9542700.1"
                     /translation="GAMTTTTDPDSIEPDAEVEPRSARTRRRLLDGALAAIDDRGFHA
                     TRVDDIVRHAGVSHGAFYLYFADKQELFRILAAEAAEDMIARVAELAADPRPTGEDLV
                     DWLDGFVAGYRRHGAVFRAWMEDHARDRGLHRLAKEAFARTVDDMGRSVDTAALGPLG
                     PVALLALIERFAYFAISRDLPTDRAALCSVATLIERGFLTEPDGVAG"
     gene            complement(629..1843)
                     /locus_tag="VFP02_06450"
     CDS             complement(629..1843)
                     /locus_tag="VFP02_06450"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HET9542701.1"
                     /translation="MSRLVLRGARQPGDIALRDGRIEAIGTVAAEPGDEMLRCDGDLV
                     TAGLINTHHHLYQWATRGRAVGCNLFDWLVELYPIWGRLSVEEIHAAALRGLTELARS
                     GCTTAADHHYLVPRGDDSVFDAIAAAAREVGIRLVLSRGSMDLGESQGGLPPDHVVED
                     RQAILASTESVIARLHDPDWCQVVVAPCSPFSVTPELMIESAELARKHGLRLHTHLCE
                     TVEETEYCLEHFGRRPVDLLDEWGWVGDDVWLAHGIHISPPEIDRLGVAGTGVAHCPS
                     SNARLAAGLCPVTDLEAAGCPVGLGVDGVASNEVGGLFPEIRQALYTARIRAGRADAF
                     LPGDALRLATAGGARCLGRPELGRLEVGAVADLAVWPADDLEDLPDPMDGLALGPDRR
                     VHHLVVDGRVVI"
     gene            complement(1840..>2358)
                     /locus_tag="VFP02_06455"
     CDS             complement(1840..>2358)
                     /locus_tag="VFP02_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9542702.1"
                     /translation="VIEGYSTMFRELGDRRFDAVFFPVGVGALAAAVVTAFRPGDTVL
                     VAVQASGAPCLMHSLEMGQVATLPGEPRTIMAGISCGTPSPVAWPLVSRGVDWVVNVD
                     DDQARDAMRVLAREGIVSGETGAAALAGLLAVLATKAEGPDLSPDSAVLVLSTEGATD
                     PVAYEEIVGRAP"
BASE COUNT          338 a          887 c          842 g          291 t
ORIGIN      
        1 ggcgccatga ccaccaccac tgatcccgac tccatcgagc ccgacgccga ggtcgagccc
       61 cgaagcgccc ggacccggcg ccgcctgctc gacggcgcgc tggccgccat cgacgaccgg
      121 ggcttccacg ccacccgcgt ggacgacatc gtgcgccatg ccggagtctc gcacggagcc
      181 ttctacctct atttcgccga caagcaggag ctgttccgca tcctggccgc cgaggcggcg
      241 gaggacatga tcgcccgcgt cgccgagctg gccgccgatc cccgccccac gggtgaggac
      301 ctggtcgact ggctggacgg cttcgtcgcc ggctaccgac gccacggcgc cgtcttccgg
      361 gcctggatgg aggaccacgc acgagaccgg ggcctgcacc gcctggccaa agaggccttt
      421 gcccggacgg tggacgacat gggccggtcg gtggacaccg cggcgctggg ccccctcggc
      481 cccgtcgccc tcctggccct gatcgagcgc ttcgcctact tcgcgatctc acgcgacctc
      541 ccgaccgacc gggccgccct ctgctcggtc gccaccctga tcgagcgcgg cttcctcacc
      601 gagcctgacg gcgtggccgg ctgacgcctc agatcacgac gcgaccgtcg acgacgaggt
      661 ggtggacgcg gcggtccggg ccgagggcga ggccgtccat cgggtcgggg aggtcctcca
      721 ggtcgtcggc gggccatacg gccaggtccg ccaccgcgcc gacctccagg cgtccgagct
      781 cggggcgtcc gaggcaccgg gcgccgcccg cggtggccag gcgcagggcg tcgccgggca
      841 ggaaggcatc ggcccggccg gctcggatgc gagccgtgta cagcgcctgg cggatctcgg
      901 ggaagagccc accgacctcg ttggaggcca cgccgtcgac gcccaacccg accgggcagc
      961 cggccgcctc gaggtcggtg accgggcaca ggccggcggc caggcgggcg ttggacgacg
     1021 ggcagtgggc cacccccgtc cccgcgaccc ccagccggtc gatctcgggc ggcgagatgt
     1081 ggatgccgtg ggccagccag acgtcgtcgc ccacccagcc ccactcgtcg agcaggtcga
     1141 ccggtcggcg accgaagtgc tcgaggcagt actcggtctc ctcgacggtc tcgcacaggt
     1201 gggtgtgcag gcgcagcccg tgcttccggg ccagctccgc cgactcgatc atcagctccg
     1261 gggtcaccga gaagggggag cacggggcga cgaccacctg gcaccagtcc gggtcgtgca
     1321 ggcgggcgat gacggactcc gtcgaggcca agatggcctg ccggtcctcg acgacgtgat
     1381 ccggcggcag cccaccttgg ctctcgccca ggtccatcga accccgggac aggaccaggc
     1441 ggatgcccac ctcccgggcg gcggcggcga tggcgtcgaa caccgagtcg tcgccccggg
     1501 gaaccaggta gtggtggtcg gcggccgtcg tgcaccccga gcgcgccagt tcggtgagcc
     1561 cccggagcgc ggcggcgtgg atctcctcga ccgacagccg cccccagatc ggatagagct
     1621 cgaccagcca gtcgaagagg ttgcagccca ccgctcggcc gcgggtcgcc cactggtaga
     1681 ggtggtggtg ggtgttgatc agcccggcgg tgacgaggtc cccgtcacag cggagcatct
     1741 cgtcgccggg ctccgcggcg acggtgccga tggcctcgat acgcccgtcc cgcagcgcga
     1801 tgtcccccgg ctggcgggcg ccccgcagga ccaaccggct cacggcgccc ggccgacgat
     1861 ctcctcgtag gcgaccggat cggtggcgcc ttcggtggac agcacgagca ccgcgctgtc
     1921 ggggctgagg tcggggccct cggccttggt cgcgagcacc gcgaggagac ccgccaatgc
     1981 ggccgccccg gtctcgcccg agacgatccc ctcgcgggcc agcacccgca tggcgtcccg
     2041 ggcctggtcg tcatcgacgt tcacgaccca gtcgacgccc cgggacacga gtggccaggc
     2101 gaccggcgac ggggtgccac acgagatccc ggccatgatc gtgcgcggct cgccgggcag
     2161 cgtggccacc tgccccatct ccagggagtg catcaagcac ggggcccctg aggcctgtac
     2221 ggccactagg accgtgtcac cggggcggaa cgcggtgacg accgccgcgg caagggctcc
     2281 gacgccgacg gggaagaaca cggcgtcgaa ccgacggtcg cccagctccc ggaacatcgt
     2341 cgagtagccc tcgatgac
//
LOCUS       DASTFV010000348         3107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1285883, whole genome shotgun sequence.
ACCESSION   DASTFV010000348 DASTFV010000000
VERSION     DASTFV010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285883"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VFP02_06460"
     CDS             complement(<1..428)
                     /locus_tag="VFP02_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542703.1"
                     /translation="MLFLALALIAGAVGFVFYMLLSDAEEKATVRSSLRQLEGYEVQD
                     VRDQELLIPLYQRALLPILKGLTALGGRFTPVGYVDTVRKKFVYAGRISADGVDRFLA
                     IRVITVVLIPAWFIFVTVFLPVTGILRLGALGLGGAILALG"
     gene            complement(441..2387)
                     /locus_tag="VFP02_06465"
     CDS             complement(441..2387)
                     /locus_tag="VFP02_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9542704.1"
                     /translation="MRILRAALGGLLTAAVLAGPAIAFAADDEAAAPYLAVRGVDSTG
                     GDVVRLTVAYEGDEGDLAGAVVTDNGSERPLSGDVVPLATTGASRAVVIAVDTSEAMD
                     PVMADIRDSVTAVVEGLPGDVPVGIVTFADRVVPVRALTTDRARILDTISQLQANGES
                     VLWDGIAGSAELLATDAPDAQPSVVVVTGSGNLASVSTSGDAVGALRNAGAPAYIVGL
                     EGARLDTGGLEDVVERAGGRLLTTGDSEALDEMGTTMAATVDQQYVLAYEPPTGDEAE
                     AVADIQVQVGDSTTAATFVRGSESDKPADLFAEAPLPVHDGLLQSDLAKFVGVGLILL
                     AAALGAFALIMLTQRDTSHLTNVLQPYSEGFVDDSSIIDDEAESGLAKTALIQRAVEM
                     TGDFAERQGFLSAVEAKLERADLPLRAAEALFFYASMVAVVGLLSLVLLQNLVGTVVI
                     TVLAIMIPKAAVNFKAKRRLKKFNNQLPDMLALLSGTLRAGYSLMQGVEAVSREVEDP
                     IGYELRRVVTESRLGRPLDEALDAAADRMGSADFRWAVMAIGIQREVGGNLAELLMTV
                     SETMTARERLRRDVDALTAEGKVSAMVLGLLPLGLGAAMFVINPEYIGVLFSEKLGNM
                     MLGGAVLLAGFGFWWMKKLIEIDV"
     gene            complement(2384..>3107)
                     /locus_tag="VFP02_06470"
     CDS             complement(2384..>3107)
                     /locus_tag="VFP02_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET9542705.1"
                     /translation="VGRLNLIVAGGTGTGKTTTLNVLSSFIPADERIVTIEDAKELQL
                     HQDHVLALEARPPNIEGRGQVTIRDLVKNALRMRPDRIVVGECRAGEALDMLQAMNTG
                     HDGSITTIHSNTPRDTLSRIETMTLMAGFDLPIRAIREQMASALDCIVQLTRLRDGTR
                     RITHVTEVQGMEGDVITLQDVFLFDYGAGVDERGKFRGHLKATGVRPKFTEKLQDLGI
                     RLGPEVFQPEPSGGHQMIGSAR"
BASE COUNT          475 a         1039 c         1135 g          458 t
ORIGIN      
        1 cccagggcga ggatggcccc gccgaggccg agggccccga ggcgcaggat ccccgtgacg
       61 ggcaggaaga cggtgacgaa gatgaaccag gccgggatga gcaccacggt gatcacccgg
      121 atggccagga agcggtccac accgtcggcg gagatgcgcc cggcgtagac gaacttcttg
      181 cgcacggtgt ccacgtagcc gaccggcgtg aaccggccgc ccagggcggt caggcccttc
      241 aggatgggga gcagggcccg ctggtagagc gggatcagca gctcctggtc acggacgtcc
      301 tggacctcgt agccctcgag ctggcgcagc gaggagcgga ccgtggcctt ctcctcggcg
      361 tcgctgagga gcatgtagaa gacgaacccg accgctccgg caatgagagc gagggcgagg
      421 aacagcatcg gtcaggcctc tcagacgtcg atctcgatga gcttcttcat ccaccagaac
      481 ccgaagccgg cgagcagcac cgcgccgccc agcatcatgt tcccgagctt ctcgctgaac
      541 aggacgccga tgtactccgg gttgatgacg aacatggccg cgccgaggcc caggggcagg
      601 agcccgagga ccatggccga gaccttgcct tccgccgtga gcgcatcgac gtcccggcga
      661 agccgctccc gggccgtcat ggtctccgac accgtcatca gcagttccgc caggttcccg
      721 ccgacttcgc gctggatccc gatggccatg acggcccacc ggaagtccgc gctgcccatc
      781 cggtccgcgg ccgcgtcgag cgcttcgtcg agcggccggc ccagacggga ctcggtcacg
      841 acccgacgca gctcgtagcc gatcgggtcc tccacttccc gggacacggc ctcgacgccc
      901 tgcatgaggg agtagccggc ccgcagcgtc ccggagagga gagcgagcat gtcggggagc
      961 tggttgttga acttcttcag tcggcgcttg gccttgaagt tgaccgcggc cttcgggatc
     1021 atgatggcca ggaccgtgat gacgacggtg ccgaccaggt tctggagcaa caccaggctg
     1081 agcaggccca cgaccgcgac catggaggcg tagaagaaca gggcctccgc cgcccggagg
     1141 ggcaggtcgg cccgctccag cttggcttcg accgcggaga ggaagccctg ccgctcggcg
     1201 aagtccccgg tcatctcgac cgcgcgctgg atcagggccg tcttggccag cccgctctcc
     1261 gcctcgtcgt cgatgatcga gctgtcgtcg acgaagcctt ccgagtacgg ctgcaggacg
     1321 ttggtcaggt gactggtgtc ccgctgggtc agcatgatca gggcgaaggc cccgagcgcg
     1381 gcggccagga ggatgaggcc gaccccgacg aacttggcca gatcactttg gagcaggccg
     1441 tcgtggacgg ggagaggcgc ttcggcgaag aggtcggccg gcttgtccga ctccgagcct
     1501 cgcacgaagg tggcggcggt cgtcgagtca ccgacctgga cctggatgtc ggccactgcc
     1561 tcggcttcgt cgccggtcgg gggctcgtag gcgaggacgt actgctggtc gaccgtcgcc
     1621 gccatcgtcg tgcccatctc gtcgagcgcc tcggagtcgc ccgtggtcag gagccggcct
     1681 ccagcccgtt cgacgacgtc ctcgagccca ccggtgtcga ggcgtgcgcc ctcgaggccg
     1741 acgatgtagg ccggtgcacc ggcgttgcgc agggccccga cggcgtcgcc ggaggtgctg
     1801 accgaggcga ggttgccgct acccgtcacc acgaccacgc tgggctgggc gtcgggtgcg
     1861 tcggtggcca ggagctcggc cgagcccgcg atcccgtccc agaggacgct ctcgccgttg
     1921 gcctgcagct ggctgatcgt gtcgaggatc cgcgcgcggt ccgtggtcag ggcccggacg
     1981 ggcacgaccc ggtcggcgaa ggtcacgatc ccgaccggaa cgtcgcccgg caggccctcg
     2041 acgaccgcgg tgaccgagtc gcggatgtcg gccatgaccg ggtccatcgc ctcggaggtg
     2101 tccacggcga tgaccacggc ccggctcgcg ccggtggtgg ccaggggcac gacgtcgccc
     2161 gagagcgggc gctcgctgcc gttgtcggtg acgacggcac cggccaggtc gccctcgtcg
     2221 ccctcgtagg caacggtcag gcgcacgacg tcgccgccgg tcgagtcgac gccacggacg
     2281 gccaggtacg gcgcggcggc ctcgtcgtcg gccgcgaagg cgatggccgg gccggccagg
     2341 acggccgcgg tgagcaggcc gccgagggcg gcgcgcagga tcctcatcgg gcggaaccga
     2401 tcatctggtg tccgccggag ggctcgggct ggaagacctc ggggccgagg cggatgccca
     2461 ggtcctggag cttctcggtg aacttggggc gcacgccggt ggccttgagg tggccccgga
     2521 acttgccgcg ctcgtccacg ccggcgccgt aatcgaacag gaacacgtcc tggagggtga
     2581 tgacgtcgcc ctccatgccc tgcacctcgg tcacgtgggt gatgcgccgg gtgccgtccc
     2641 gcagtcgggt cagctgcacg atgcagtcca gagcggaggc catctgctcc cggatggccc
     2701 ggatcggcag gtcgaaaccg gccatcaggg tcatcgtctc gatacgggac agggtgtccc
     2761 gaggcgtgtt ggagtggatg gtcgtgatcg agccgtcgtg gcccgtgttc atggcctgga
     2821 gcatgtccag ggcctccccg gcgcgacact ccccgaccac gatccggtcg ggacgcatac
     2881 gcagggcgtt cttgacgagg tcacggatgg tgacctggcc ccgaccctcg atgttgggcg
     2941 ggcgggcctc cagggccagg acgtggtcct ggtggagctg gagctccttg gcgtcctcga
     3001 tggtgacgat gcgctcgtcg gcggggatga aggaggagag cacgttcagc gtcgtggtct
     3061 tgccggtacc ggtaccgccg gcgacgatca ggttcaggcg cccgacg
//
LOCUS       DASTFV010000349         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1288994, whole genome shotgun sequence.
ACCESSION   DASTFV010000349 DASTFV010000000
VERSION     DASTFV010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288994"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..824)
                     /gene="extP"
                     /locus_tag="VFP02_06475"
     CDS             complement(45..824)
                     /gene="extP"
                     /locus_tag="VFP02_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HET9542706.1"
                     /translation="MAKEIAKRPNAGELVGQKLDDFQGGQVWSSIFRPGSIFRKGYTD
                     SPRNRSYVIMNSVLYHLHPVKVKRHAVKVSYTLCLGGLSFFLFILLTVTGIFLMFFYR
                     PTATQAWDDIQALHTSVAFGLLVRNMHRWAAHLMVISVFLHMARVFYHGAYKPPREFN
                     WVIGVVLLLLTLLLSFTGYLLPWDQLALWAVTVGSNMGGFAPVFGSQVKFALLGGAEI
                     GSETLLRWYVLHVLFLPFVIVIFMAVHFWRVRKDGGISGPL"
     gene            complement(852..1268)
                     /locus_tag="VFP02_06480"
     CDS             complement(852..1268)
                     /locus_tag="VFP02_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET9542707.1"
                     /translation="MTTTDANPPMPEFRADYVLQEVDAAWLGQVVKPKQFIHIDQSEC
                     IMCEGCVDICPWKCIHMVTPDAIDESMGTELPGLDPSDHVIFTIDDDVCTRCALCVDR
                     CPTGVIILGKIGAPASTGDAHQRTNAHGYAYGMRLG"
     gene            complement(1273..1680)
                     /locus_tag="VFP02_06485"
     CDS             complement(1273..1680)
                     /locus_tag="VFP02_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542708.1"
                     /translation="MLVSIALIGGGIFYMHRRPVEPKGTWGESMVGAVYVFLALFWCF
                     GVVPHQWILYADSGLAWSVDRYLVGPGSILENLPFVIPYSALRDIMVVNINVVYVIVW
                     AKVWSSWQSRGKAEPVAETVTSDYGRPLVKAEA"
     gene            1937..>2289
                     /locus_tag="VFP02_06490"
     CDS             1937..>2289
                     /locus_tag="VFP02_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542709.1"
                     /translation="MVTEVPDYLLQRSRERRAALGLGGDEEGGALAASGDAGGDAPAT
                     TSAASGTPAVAAPAPIAPGVPDPEPSAPPPNVQAALRRKRVPIWALPVIVLLPLWGYV
                     YAGTLEPPTSEALGAL"
BASE COUNT          401 a          775 c          770 g          343 t
ORIGIN      
        1 agcttcctcc gcccgcttcc gcaaatgttc cggaacttca gtcatcagag cggcccgctg
       61 attccgccgt ccttgcggac ccgccagaag tggaccgcca tgaagatgac gatcacgaag
      121 ggcaggaaca gcacatgcag cacgtaccac cgcaacaacg tctccgagcc gatctcggcc
      181 ccaccgagaa gggcgaactt gacctgcgac ccgaagaccg gggcgaaccc acccatgttc
      241 gacccgaccg tcacggccca gagcgccaac tggtcccagg gcaggaggta gccggtgaag
      301 ctcaggagca gggtcagcag cagcaggacc acgccgatga cccagttgaa ctccctgggc
      361 ggcttgtagg cgccgtggta gaagacccgg gccatgtgga ggaagaccga gatcaccatc
      421 aggtgcgcgg cccaccggtg catgttccgc accagcaggc cgaaggccac cgaggtgtgc
      481 agggcctgga tgtcgtccca cgcctgggtc gcggtcgggc ggtagaagaa catcaggaag
      541 atgccggtca cggtcagcag gatgaagagg aagaagctca gcccaccgag gcagagcgtg
      601 tagctgacct tcaccgcgtg ccgcttcacc ttcaccgggt ggaggtggta caggacgctg
      661 ttcatgatga cgtaggaccg gttccggggg ctgtccgtat agcccttccg gaagatcgag
      721 ccgggtcgga agatcgagct ccagacctga ccaccctgga agtcgtcgag cttctggccg
      781 accagctcgc ccgcattggg ccgcttcgcg atttctttcg ccacggtgag ggggctcctt
      841 gatctacctg gtcagccgag gcgcatgccg taggcgtaac cgtgggcgtt ggtgcgctgg
      901 tgcgcgtcgc cggtggaggc gggggcgccg atcttgccca ggatgatgac tccggtcggg
      961 caacgatcga cgcacagggc gcaccgggtg cagacgtcgt cgtcgatcgt gaagatcacg
     1021 tggtcactgg ggtccaagcc gggcagctcg gtgcccatcg actcgtcgat ggcgtcgggc
     1081 gtgaccatgt ggatgcactt ccacgggcag atgtccacgc agccctcgca catgatgcac
     1141 tcggactggt cgatgtggat gaactgcttg ggcttgacca cctgaccgag ccacgccgcg
     1201 tccacttcct gcaggacgta atcggcccgg aactcgggca tcgggggatt ggcgtcggtg
     1261 gtcgtcatcg gctcacgcct cggccttgac gaggggtcgg ccgtagtcgg acgtcacggt
     1321 ctcggcgacg ggctcggcct tgccccggct ctgccaggag gaccagacct tggcccagac
     1381 gatcacgtac acgacgttga tgttgacgac catgatgtcc cggagcgccg agtacgggat
     1441 gacgaagggg aggttctcga ggatggaccc ggggccgacc aggtacctgt cgacgctcca
     1501 ggcgaggccg gagtcggcgt acaggatcca ctggtgcggg accacgccga agcaccagaa
     1561 gagggcgagg aagacgtaga cggcgccgac catcgactcg ccccaggtgc ccttgggctc
     1621 gaccgggcga cggtgcatgt agaagatgcc cccaccgatg agggcgatgc tgacgagcac
     1681 ggaggcaacg aacgcaacca cctgcctgtc acctcctcgg ggtctgggtc accggttcgg
     1741 acttcgtgaa cgcaatcaca atcgtccggc ccaaggctag ccgccgggcc tcgatctgtg
     1801 gtctagcacg gcacccccgg ccgaaacaca tcgggagcgg gcctccctcc aggcggcgaa
     1861 acgggcgccc cgctgcttgg tcccgggcgg tggcgtgggc ctagagtgac caggcccacg
     1921 cagactcacc ggaggcatgg tgaccgaggt tcccgactat ctgctccagc gctcgcgtga
     1981 gcgccgtgcc gccctcggtc tcggcggcga cgaggagggt ggagccctcg ccgccagcgg
     2041 cgacgccgga ggtgatgccc cggccaccac ctccgccgcc tcgggcaccc ccgccgtggc
     2101 cgccccggcc ccgatcgccc cgggcgtgcc cgatccggaa ccgtcggccc ccccacccaa
     2161 cgtccaggcc gccctgcggc gcaagcgggt cccgatctgg gccctgcccg tgatcgtgct
     2221 cctcccgctc tggggctacg tctacgccgg caccctcgag ccccccacca gcgaggccct
     2281 cggcgccct
//
LOCUS       DASTFV010000350         6539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1295852, whole genome shotgun sequence.
ACCESSION   DASTFV010000350 DASTFV010000000
VERSION     DASTFV010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295852"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFP02_06495"
     CDS             complement(<1..282)
                     /locus_tag="VFP02_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542710.1"
                     /translation="MSEPHTSGWAVALQASDRVAFKRCRRAWDFGSPARRNLVPRTPA
                     PRDGLGPALHEALAVYYFPGMWEWPREIVEPLAREAFLGAFGEHAGVDDI"
     gene            complement(279..2570)
                     /gene="hypF"
                     /locus_tag="VFP02_06500"
     CDS             complement(279..2570)
                     /gene="hypF"
                     /locus_tag="VFP02_06500"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HET9542711.1"
                     /translation="MSVGRRIRVTGTVQGVGFRPFVYREAVRLGLVGTVRNDHAGVLI
                     EVDGAPDRVAELCRCLVDRPPPLARIERLEASDLVHVAEPVGGGFAIVASEGLGSPEV
                     PVGVDAATCAECLAEVTDPTDRRFGYPFANCTNCGPRYTIVVSVPYDRPATTMASFPL
                     CAACRQEYDDPSDRRFHAQPNACPVCGPRLAWRSVSDDPGVTEGEALTAAAACLAAGG
                     TVAVKGLGGYHLAVDATSRAAVTTLRRRKHRDEKPFAVMVPDVVTARELVVLDDGAEA
                     VLTSSRRPIVLAPRRPGAPQVADAVAPGLPELGLLLAYTPLHNLLLQAVGRPLVMTSG
                     NVSDEPIAQADDDAFARLAPLVDGWLSHDRPIHIRCDDSVVRAVAGRTQVLRRSRGYA
                     PEPMALGHVACRQVLAVGAELKSTVSVVKGQQLVTSHHIGDLEHPTAYASFRQAVEHL
                     CGLFGVRPDVVVHDLHPEYLSTKWALDTDLPTLGVQHHHAHVASCLAEHRHPGPVLGV
                     AFDGLGYGCDRTLWGGEFLAADLDGCTRLGHLRPVALPGGVAAVREPWRMAVAWVYAA
                     MGAYRAEREATRLDERGRALIGLLEAGSAPTTTSVGRLFDAVAALLGLRSRLTYEGQA
                     AIELEALARTVPRGQAPALPVGLGVEGTTPVLDPGPLIAAVLAGRDDGVPLAVLAAGF
                     HEGLGQAAARLADDLARAQDLDTVVLTGGVFQNTRLTEVVESALATAGLRVLLHREVP
                     PNDAGISVGQAAIAARTTRIDGR"
     gene            complement(2576..3667)
                     /gene="hypE"
                     /locus_tag="VFP02_06505"
     CDS             complement(2576..3667)
                     /gene="hypE"
                     /locus_tag="VFP02_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HET9542712.1"
                     /translation="MSEPVADVREAAVLERIEAFRRRRPRFKDEVVTLAHGAGGKASA
                     ALVDAVFLEAFNDEVPTVLPDGAVLALPSGERLAFSTDSFVVSPTRFPGGSIGDLAVN
                     GTVNDVAMMGGRPQWVSAAFVLEEGLAMDVLRDVVEDMAQAARRAGVRVVTGDTKVVN
                     GGAADGLYITTAGVGIIPVGRELGPGRVRPGDRVIVSGTLADHGMAVMLARGDLALEA
                     DIRSDTAPLTALVDLLFAAAPGVRWLRDPTRGGLGTVANELARDCGLAVVLDEASLPV
                     DPAVNGACDLLGIDPLYVANEGKLVAVVGPDEADAAVRALRGHALGARAAVIGEVVAE
                     PEGLVVLQTSFGGTRIVDMLVGDPLPRIC"
     gene            complement(3664..4317)
                     /locus_tag="VFP02_06510"
     CDS             complement(3664..4317)
                     /locus_tag="VFP02_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET9542713.1"
                     /translation="MSPEATDFLYPFIEGDEQDTRSLLADLEASARVKSEESLRLAEV
                     TLEEYGDLLDTVAEALAARSRRGGVLFTFGNGGSATDAQRIAGLFAGPPVGLAVAARC
                     LVDDPAVLTALSNDVGFDLVFSRQLIAHGSSRDTALGLSTSGDSANLLHAFEEARRRG
                     LLTIAVVGGGGGRCATSPDVDHCLVVRSGSVHRIQEVQAEVVLDLWTRVQRCLGVSP"
     gene            complement(4314..5036)
                     /locus_tag="VFP02_06515"
     CDS             complement(4314..5036)
                     /locus_tag="VFP02_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HET9542714.1"
                     /translation="MPSAATTEAVPATELAVAALALARRFAAGATLWCVAPQWPDHAR
                     HVAVEFVHPVVVGTRALPAVAVDRPEALAALRGSVRAGDALIAIAPAADPVVSDLARR
                     APAWGVETFWVGGGPRPGAGAADHVLWSAEDDPLLAHSSRFVVAYHVLWEMTHVCFEH
                     PGLLTSPAPACREDGCITCSDEGRLAEVLTVDDHDVAVVRTADGEEGVDVSLVTPVAS
                     GDLVLVHAGTALTRVASEGADS"
     gene            5168..>6539
                     /locus_tag="VFP02_06520"
     CDS             5168..>6539
                     /locus_tag="VFP02_06520"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HET9542715.1"
                     /translation="MGAIRNFESHLISRTTFGWTPQTERAVLDAGWVRWLDAQLAPAT
                     IPDPAIAPLLAGYQTLANSNWQNAVLRDSTDDGWDRIVRERAHATFLRARYSRRQLYE
                     VMVEFWTNHFNVNLDDTLADHMITVDNREVARAHALGRFVDLLQASAHSPAMLLYLDN
                     ATSDANSDEGVNENYGRELLELHTMGIIDGVQPYTEADMRGVALTLSGWSVNTDDGQH
                     RFQFKTDMHHAGPVNILGGTFVTSGHTGSAGLADGVRLLNIIARHPATARYIAYKLCR
                     RFVADTPPPALVASAAAVYTQNDTAIAPVLRHIFHSAAFASGPRNKVRRGFEVFVAYA
                     RAMGGTAEPDPLGDLANHLHGYGWGFLERLGQRLWGHRLPDGYPDTAPDWISADGLLR
                     RWETAGALTATWLDGWTFDAHAILEPTGPTVGTWIEGVARRLLGVVTGYPDHGYRDVP
                     AWAEDSV"
BASE COUNT         1029 a         2420 c         2302 g          788 t
ORIGIN      
        1 gatgtcatcg acccccgcgt gctccccgaa ggccccgagg aaggcctcgc gggccagtgg
       61 ctcgacgatc tcacggggcc actcccacat gccggggaag tagtagacgg cgagcgcctc
      121 gtggagcgcc ggccccagcc cgtcacgcgg agcgggcgtg cgggggacga ggttgcggcg
      181 ggccggggac ccgaagtccc atgcccggcg acagcgcttg aaggcgacgc ggtccgacgc
      241 ctgcagggcc acagcccacc cggaggtgtg gggttcgctc atcgaccgtc gatccgggtc
      301 gtgcgggcgg cgatcgccgc ctgaccgacg ctgatgccgg cgtcgttggg ggggacctca
      361 cggtgcagga ggacccggag gccggcggtg gccaacgccg actcgaccac ctcggtgagc
      421 cgcgtgttct ggaacacccc gcccgtcagg acgaccgtgt ccaggtcttg ggccctggcc
      481 aagtcgtcag cgagccgggc ggcggcctgc ccgagtccct cgtggaaacc agccgccagc
      541 accgcgagag ggacgccgtc gtcacggccc gccagcaccg cggcgatgag gggaccgggg
      601 tcgaggacgg gggtcgtccc ctccacgccg aggcctacgg gcagggccgg cgcctgaccc
      661 cgcggcacgg tccgggccag ggcctccaac tcgatggccg cctggccctc ataggtgagg
      721 cggctccgca gacccagtag ggcggcgacg gcgtcgaaca gccgacccac actggtcgtc
      781 gtcggcgccg aacccgcctc gaggagaccg atgagcgccc ggccccgctc gtccagccgc
      841 gtcgcctccc gctccgcccg gtacgcgccc atggccgcgt acacccacgc caccgccatg
      901 cgccatggct cccgcaccgc ggcgacacca ccaggcaggg cgacggggcg gagatggccg
      961 agccgggtgc acccgtcgag gtcggccgcg aggaactccc cgccccagag cgtgcggtca
     1021 cagccgtagc cgaggccgtc gaaggccacc ccgagcaccg gaccggggtg acggtgctcc
     1081 gccaggcacg acgcgacgtg ggcgtggtgg tgctgcaccc cgagggtcgg caggtccgtg
     1141 tccagggccc acttcgtgct gaggtactcg ggatggaggt cgtgcacgac cacgtcaggg
     1201 cgcacgccga agagtccgca gaggtgctcg acagcctgcc ggaacgaggc gtaggccgtc
     1261 gggtgctcga ggtcgccgat gtgatggctg gtcaccagct gctgaccctt gacgacggag
     1321 accgtgctct tgagctcagc tcccaccgcc agcacctggc ggcaggcgac atgaccgagg
     1381 gccatgggct ccggcgcgta gccccgcgac cgacgcagca cctgcgtccg cccagccacg
     1441 gcccggacga ccgagtcgtc gcagcggatg tggatcgggc gatcgtggct gagccaaccg
     1501 tccaccagag gcgcgaggcg ggcgaaggca tcgtcgtcgg cctgcgcgat cggttcgtcg
     1561 ctcacgttcc cgctggtcat gaccaaaggc cgaccgacgg cttgcaggag aaggttgtgc
     1621 aagggggtgt aggccaggag gaggcccaac tctggcaggc ccggtgctac cgcgtcggca
     1681 acttgcgggg cgcccggtcg gcggggggcc agcacgatcg ggcgccgcga cgacgtgagc
     1741 acggcctcgg ccccgtcgtc gagcacaacc agttcccggg ccgtcacgac atcggggacc
     1801 atcaccgcga acggcttctc atcccggtgc ttccgccggc gcaacgtggt gacggccgcg
     1861 cgggaggtcg cgtccaccgc gaggtgatag ccgcccagac ccttcacggc gacggtcccc
     1921 ccggcggcca agcaggcagc cgcggcggtc agggcctcac cctccgtcac tccgggatcg
     1981 tccgagaccg accgccaggc gaggcgaggc ccacagaccg gacaggcatt gggctgggcg
     2041 tggaaccgac ggtcggaagg gtcgtcgtac tcctgccgac aggccgcgca cagggggaac
     2101 gaggccatcg tggtggcggg ccggtcgtag ggcaccgaga cgacgatggt gtagcgggga
     2161 ccgcagttcg tgcagttggc gaacgggtag ccgaagcgcc ggtccgtcgg gtccgtgacc
     2221 tcggccaggc actcggcaca ggtcgccgca tcgacgccga ccggaacctc cggggagccg
     2281 aggccctcac tggcgacgat ggcgaacccc ccaccgaccg gctcggcgac gtggaccagg
     2341 tcgctggcct cgaggcgctc gatgcgggcc agcggtggcg ggcgatccac caggcatcgg
     2401 cacagttcgg cgacgcgatc gggcgctccg tccacctcga tgagcacgcc ggcgtggtcg
     2461 ttgcgcacgg ttccgaccaa tccgagacga acggcttcgc ggtacacgaa cggtcggaac
     2521 ccgacgccct ggaccgtgcc cgtgacccgg atgcgccgcc cgaccgacac ggcgctcagc
     2581 agatgcgcgg gagcggatcg cccacgagca tgtccacgat gcgggtcccg ccgaacgagg
     2641 tctgcaggac gaccaggccc tccggctcgg ccaccacctc gccgatcacc gcggcccgcg
     2701 cgcccagggc gtgaccgcgg agggcgcgca ccgcggcgtc ggcctcgtcg gggccgacga
     2761 cggccaccag cttcccctcg ttggccacgt agagcggatc gatccccagc aggtcgcagg
     2821 ccccgttgac ggccgggtcc acgggcaacg acgcctcgtc gagcaccacg gccaacccgc
     2881 agtcccgggc cagttcgttg gcgaccgttc cgagcccgcc gcgggtcggg tcccgaagcc
     2941 agcgcacgcc gggcgcggcg gcgaaaagca ggtcgaccaa cgccgtcaac ggggcggtgt
     3001 cggagcggat gtcggcttcg agggcgagat cgccgcgggc cagcatcacg gccatgccgt
     3061 ggtcagcgag cgtccccgac acgatcacgc ggtcgccggg gcgaacccgt cccggcccga
     3121 gctcgcgccc gacagggatg atcccgacgc cggcggtcgt gatgtacaag ccgtcggcgg
     3181 cgcccccgtt gaccaccttg gtgtcgccgg tcacgacccg gaccccggcc cgccgggccg
     3241 cctgcgccat gtcctccacg acatcccgaa ggacatccat ggccaggccc tcctccagta
     3301 cgaaggcggc ggacacccac tgtgggcgac cacccatcat ggccacgtcg ttgaccgtgc
     3361 cgttgaccgc gagatccccg atggagccac ccgggaaccg cgtcgggctc acgacgaagg
     3421 agtccgtgct gaaggcgagg cgctcacccg agggcagcgc gagcacggcg ccgtcgggca
     3481 ggacggtcgg cacctcgtcg ttgaacgcct cgaggaacac ggcgtccacg agtgcggccg
     3541 aggccttgcc accggcaccg tgggccaggg tgaccacctc gtccttgaac cgagggcggc
     3601 ggcggcggaa cgcctcgatg cgctcgagca ccgcggcctc acggacgtcg gcgacgggct
     3661 cgctcacggc gacaccccga ggcaccgctg cacccgcgtc cagagatcga gcaccacctc
     3721 ggcttgcacc tcctggatgc ggtgcacgct ccccgatcgc accaccaggc agtggtcgac
     3781 gtccgggctg gtcgcgcacc gaccaccgcc accgccgacc acggcgatgg tgagcagccc
     3841 gcgccgtcgg gcctcctcga acgcgtggag gaggttggcc gagtcgccgc tggtcgagag
     3901 gcccagggcc gtgtcccgcg agctcccgtg ggcgatgagt tgccgggaga agacgaggtc
     3961 gaagccgaca tcgttgctga gggcggtgag gacagccgga tcgtcgacca gacagcgggc
     4021 ggcgaccgcg agaccgacgg ggggccccgc gaaaaggcct gcgatgcgct gggcatccgt
     4081 ggcgctcccg ccgttgccga aggtgaagag gacacccccc cggcgggaac gcgccgccag
     4141 ggcctcggcg accgtgtcca agaggtcgcc gtactcctcg agggtgacct cggcgaggcg
     4201 gaggctctcc tcgctcttca cccgggcgga cgcctcgagg tcggcgagga gcgatctggt
     4261 gtcctgctcg tcgccctcga tgaagggata gaggaagtcg gtcgcctcgg ggctcacgag
     4321 tccgcgccct cggacgcgac ccgggtcagc gccgtcccgg cgtgcaccag gaccagatcc
     4381 ccgctcgcca ccggggtgac caggctcacg tccacccctt cctcgccgtc ggctgtgcgg
     4441 accacggcca cgtcgtgatc gtccaccgtc aggacctcag ccagtcgacc ctcgtccgag
     4501 caggtgatgc acccatcctc ccggcaggcg ggagccgggc tcgtcagcag gcccgggtgc
     4561 tcgaagcaga catgggtcat ctcccagagc acgtgatagg ccacgacgaa ccggctggag
     4621 tgcgccagga gcgggtcgtc ctcggcgctc cacagaacgt ggtcggcggc gccggcgccg
     4681 ggtcgaggcc cgccgcccac ccagaaggtc tcgacgcccc acgccggcgc ccggcgcgcc
     4741 agatccgaca cgaccgggtc ggcggccggg gcgatggcga tcagcgcatc accggcacgg
     4801 acggaccccc ggagggcggc caatgcctca ggtcggtcca ccgccactgc gggaagggcg
     4861 cgggtgccca ccaccacggg atggacgaac tcgacggcga cgtgacgggc gtgatccggc
     4921 cattgcggag caacacacca aagggtcgcc ccggccgcga atcggcgcgc cagagccaat
     4981 gccgccacgg ccagttcagt ggcgggaacg gcctcggtcg tggccgcaga gggcatcgtc
     5041 cccggagtct gacacgcccg gacgccggtc cgaatagggg gaaaatgcac ctagtcgccc
     5101 gaggctccgg accgcacgga gctgaatggc ctcaagggcc gtggtcggtc ggtcgacgtt
     5161 ggtcgccgtg ggcgcgatcc gcaacttcga gagccacctc atctcccgca ccaccttcgg
     5221 ttggacgccg cagaccgagc gcgccgtgct cgacgcgggc tgggtgcgct ggctcgacgc
     5281 ccaactcgcc cccgcgacga tccccgaccc ggcgatcgca ccactcctcg ccggctacca
     5341 gaccctggcc aactcgaact ggcagaacgc cgtcctcagg gacagcaccg acgacgggtg
     5401 ggaccggatc gtccgcgagc gggcccacgc caccttcctg cgagcccgct acagccgccg
     5461 ccagctctac gaggtgatgg tcgagttctg gaccaaccac ttcaacgtga acctggacga
     5521 caccctggcc gaccacatga tcaccgtcga caaccgagag gtcgctcggg cccacgccct
     5581 cggacggttc gtcgacctcc tccaggccag cgcccacagc ccggcgatgc tcctctacct
     5641 cgacaacgcg acaagcgacg ccaacagcga cgagggagtg aacgagaact acggccgcga
     5701 gctgctcgag ctgcacacga tggggatcat cgacggcgtc cagccgtaca ccgaggccga
     5761 catgcgcggg gtggccttga cgctgtcggg ctggtcggtg aacaccgacg acggccagca
     5821 ccggttccag ttcaagaccg acatgcacca cgcggggccg gtcaacatcc tcggggggac
     5881 gttcgtcacc tcaggccaca ccgggtcggc aggcctggcc gacggggtca ggctgctcaa
     5941 catcatcgcc cgccacccgg ccacggcccg ctacatcgcc tacaagctgt gtcgccgctt
     6001 cgtcgccgac accccaccgc cggcgctggt cgccagcgcg gccgcggtct acacgcagaa
     6061 cgacaccgcc atcgccccgg tcctgcgcca catcttccac tcggcggcgt tcgcgtccgg
     6121 gccccgcaac aaggtccggc gggggttcga ggtcttcgtc gcctacgccc gcgcgatggg
     6181 gggcaccgcc gagcccgatc ccctcggcga cctggccaat cacctgcacg gttacggctg
     6241 gggcttcctc gagcgcctcg ggcagcgcct gtggggccac cgcctgcccg acgggtaccc
     6301 ggacaccgcc cccgactgga tctccgccga cgggctgctc cgacgctggg agacggcggg
     6361 cgcgctcacg gccacctggc tggacggatg gaccttcgac gcccacgcca tcctggagcc
     6421 gaccggcccg acggtcggca cgtggatcga aggcgtggcc cgccgcctgc tcggtgtggt
     6481 caccggctac cccgaccacg gctaccgcga cgtacctgcc tgggccgagg actcggtcc
//
LOCUS       DASTFV010000351         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1296975, whole genome shotgun sequence.
ACCESSION   DASTFV010000351 DASTFV010000000
VERSION     DASTFV010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296975"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..680)
                     /gene="tmk"
                     /locus_tag="VFP02_06525"
     CDS             complement(81..680)
                     /gene="tmk"
                     /locus_tag="VFP02_06525"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET9542716.1"
                     /translation="MVGRFIALEGGEGCGKSTQAQRLADHLSARLTREPGGTPAGERL
                     RDIVLDPATGELDFRAEVLLIAAARADHVARVIRPALEGGEDVVSDRYVASSLAYQGH
                     GRRLGVEDVARINEWATGGLKPDLTILIDVPEEVAAQRMDTDLDRLEAVGDGFHGRVA
                     DGYRALAAADPGGWAVIDGTGTVDEVFARVLAAVEERLS"
     gene            complement(686..>2261)
                     /locus_tag="VFP02_06530"
     CDS             complement(686..>2261)
                     /locus_tag="VFP02_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HET9542717.1"
                     /translation="EVQRDLQGDEFRLYELVWRRTVASQMTDARGQSVQVRLGATASD
                     GRDAEFAATGKTIHHPGFLRAYVEGSDDPEGDLEDQERILPDMAEGDRVAVRSIEAKG
                     HETQPPARFTEASLVRRLEELGVGRPSTYASIIGTIQDRGYVWKKGSALVPSFTAFAV
                     ITLLETHFPDLVDYAFTARMEDELDEIASGTAEQIPWLSRFYFGEGGKDGPGVGLQDM
                     VSNNLGDIDARAVNTIPIGADADGVEIVARPGRYGPYLQRGEDTASIPEDLPPDELTP
                     AKAVELLEAPSDDRVVGQDPETGLSVLARNGRFGPYVQLGDLESGEVEGKPKTASLFQ
                     TMTVNTLTLDEALQLLSLPRTVGVDPADGVEITVQNGRYGPYLKKDKDTRSLTDEDQL
                     FTIDLEACLALLAQPKTRGRGAAKPPLKELGEDPATGEPMILKDGRFGPYVTDGTSNA
                     SLRKGDTIESITPERGAELLAERRLKAPPKKKAAAKKKPAAKKKPAAKKKPAAKKPAE
                     AEASEPVEPAPAGASD"
BASE COUNT          265 a          813 c          828 g          355 t
ORIGIN      
        1 cggggcggcg accgcggccc gcaggtcggc ctccgccctc gcctggccga ccacgtcgcc
       61 ccagacgtcc acgtccacgc tcacgagagg cgctcctcga cggcggcgag gacgcgggcg
      121 aagacctcgt cgacggtgcc ggtgccgtcg atgacggccc aacccccggg atcggcggcg
      181 gccagagccc ggtagccgtc cgccacccgg ccgtggaacc cgtcgcccac cgcctcgagc
      241 cggtcgaggt cggtgtccat ccgctgcgcg gccacctctt ccgggacgtc gatcaggatg
      301 gtgaggtcgg gcttcaggcc gccggtggcc cattcgttga tccgggccac gtcctccacg
      361 ccgagccggc ggccatggcc ctggtaggcc agcgacgagg ccacatagcg gtcgctcacg
      421 acgtcctcgc cgccctcgag cgccggccgg atcacccggg cgacgtggtc ggcccgagcg
      481 gcggcgatga gcaggacctc ggcccggaag tccagctcgc cggtggcggg gtccagaacg
      541 atgtcccgca ggcgctcgcc ggccggcgtc ccgcccggct cgcgggtgag ccgggcgctc
      601 aggtggtcgg ccagacgttg ggcctgggtc gacttgccgc agccctcgcc gccctcgagg
      661 gcgatgaacc ggccgaccac cggtgtcagt cgctcgcccc ggccggtgcg ggctcgaccg
      721 gctcgctcgc ctcggcctcg gccggcttct tcgccgccgg cttcttcttc gccgccggct
      781 tcttcttggc cgccggcttc ttcttggccg cggccttctt cttcggcgga gccttgaggc
      841 ggcgctcggc caggagctcg gcgccccgct cgggcgtgat gctctcgatc gtgtcaccct
      901 tgcgcagcga ggcgttgctc gtgccgtcgg tgacgtaggg cccgaagcga ccgtccttca
      961 ggatcatcgg ctcgccggtc gccgggtcct cgcccagctc cttcagcggg ggcttggccg
     1021 ccccccggcc ccgggtcttg ggctgggcca gcagggccag gcaggcctcg agatcgatcg
     1081 tgaagagctg gtcctcgtcg gtgaggctgc gggtgtcctt gtccttcttc aggtacgggc
     1141 cgtagcgacc gttctgcacc gtgatctcga cgccgtcggc cggatccacc ccgacggtcc
     1201 ggggcaacga caagagctgc agcgcctcgt ccagggtgag ggtgttcacg gtcatggtct
     1261 ggaacagcga cgccgtcttg ggcttgccct cgacctcacc ggactcgagg tcaccgagct
     1321 ggacgtaggg cccgaaccgt ccgttccggg ccagcacgct caagccggtc tcggggtcct
     1381 ggccgaccac ccggtcgtcg ctgggggcct cgaggagctc gacggccttg gccggggtga
     1441 gctcgtcggg cggcaggtcc tcggggatgg acgcggtgtc ctcaccccgc tggaggtagg
     1501 ggccgtagcg cccggggcgg gccacgatct cgacgccgtc ggcatcggcc ccgatgggga
     1561 tggtgttgac ggcgcgggcg tcgatgtcgc cgaggttgtt cgacaccatg tcctggaggc
     1621 cgacgccggg gccgtccttg ccgccctcgc cgaagtagaa gcggctcagc cacgggatct
     1681 gctcggcggt gccgctggcg atctcgtcca gctcgtcctc catgcgggcg gtgaacgcgt
     1741 agtcgacgag gtcggggaag tgggtctcca ggagggtgat cacggcgaag gcggtgaagg
     1801 acggcaccag ggccgacccc ttcttccaca cgtagccgcg gtcctggatg gtgccgatga
     1861 tcgaggcgta ggtcgacggg cgtccgaccc cgagctcctc caggcggcgg accagcgagg
     1921 cctcggtgaa gcgggcgggg ggctgggtct cgtggccctt ggcctcgatg ctgcgcacgg
     1981 ccacccggtc gccctcggcc atgtcgggca ggatccgctc ctggtcctcg aggtcgccct
     2041 cgggatcgtc ggagccctcg acgtaggccc gcaggaaccc cgggtggtga atggtcttgc
     2101 cggtggccgc gaactcggcg tcacggccgt cgctcgccgt ggccccgagc cgcacctgaa
     2161 cgctctggcc ccgggcgtcg gtcatctggg aggcgacggt gcggcgccag accagctcgt
     2221 agagacggaa ctcgtcgccc tgcaggtcgc gctggacctc c
//
LOCUS       DASTFV010000352         4066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1297365, whole genome shotgun sequence.
ACCESSION   DASTFV010000352 DASTFV010000000
VERSION     DASTFV010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297365"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2335)
                     /locus_tag="VFP02_06535"
     CDS             complement(<1..2335)
                     /locus_tag="VFP02_06535"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HET9542718.1"
                     /translation="MSALLAAEGGYQSFELGGGEWFWLIFSAVTALIALAVGFYLVRA
                     VNAADTGTDKMREIAAAIQEGAMAYLKRQFKTIAIIVIPLAIIVFITSTEVVKPTDAL
                     GYVSGLSQFESGLFRTLAFLAGCFLSGLTGFIGMSLAVRGNVRTAAAARSGSMPEALE
                     VAFRTGGVAGMFTVGLGLLGATVIIMIFQNTSSAILIGFGFGGSLLALFLRVGGGIFT
                     KAADVGADLVGKVEAGIPEDDPRNPATIADNVGDNVGDCAGMASDLFESYEVTLVASI
                     ILGVAAFESIGELNPVVGMIFPVAARAIGVLASIAGVFSVRATEKDKSAMAPINRGFL
                     TATILTIIGTGIVALVYVGNDPGDPSAAGYVSNPGWRMLGAVVIGLILAQVVSRLTEY
                     FTSTEHTPVRDIAEAARTGPATTVLSGISSGLESSVWAIVAIAVALGAVIGLGGGNIQ
                     FVFYLVALAGMGMLATTGVVVSEDTFGPVADNAAGIAEMSGEFSGEAERIMVSLDAVG
                     NTTKAVTKGFAIGSAVIAAVALFASYIETVADELPDLVGPGQNLFEAIPINVADPKTF
                     IGLLIGGSIAFMFSSLAIRAVGRTAAVVVQEVRRQFADGKIMSGEKRPDYGPVIDICT
                     TASLRELATPALLAVLTPVIIGFGVNFYALGAFLAAVILTGQLMANYQNNAGGAWDNA
                     KKYIEDGAEGGKGSDAHKAAVIADTVGDPFKDTAGPALNPLIKVMNLVSLLILPAIIS
                     LSNVGDADEPLGWLKSTPKAGGYAVAAVALAVLLGAIA"
     gene            2608..3789
                     /locus_tag="VFP02_06540"
     CDS             2608..3789
                     /locus_tag="VFP02_06540"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET9542719.1"
                     /translation="MPSPSFRSLTRGVLAVVAATTMVVVPVLVQAEESDDPRAERDRV
                     REQAADVAAQLDGLQADAQEVGDALRALADNVQSKRAQFQDAQRAAATALAEARAARA
                     EEQRLQDQIADLEDQVAQIAVEAYIRPPESNDAAVYETSDINDTTLRAALLGTQSNRQ
                     EDVIDQLRAAEDDLSAQRVAAEAAAQEARALRGQTRTQLLELRAAQNQQRELARQVDE
                     RINQAAGEAAALAERDEELSDQIAEEAAQFASFIQGLNLGPVGPSYQSGPVSVADAGN
                     GIIVNVAIVDQVHNMIVAAANDGVTLTGGGYRDSAQQIALRRAHCGSSDYAIYQAPSS
                     SCSPPTAIPGTSMHEQGLAIDFDNCSYGTSVYNWLSGHAAGYGLYNLPSESWHWSTTG
                     G"
     gene            3823..>4066
                     /locus_tag="VFP02_06545"
     CDS             3823..>4066
                     /locus_tag="VFP02_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HET9542720.1"
                     /translation="MDLTVATETVDGIWEDSALAALTDYIRIPAKSPAYDADWVAHGH
                     LADAVELVAAWCRSRDLPGATVEIRELPGRTPVVLVE"
BASE COUNT          672 a         1407 c         1421 g          566 t
ORIGIN      
        1 atgcgatcgc gcccagcagg accgccagcg ccacggccgc caccgcgtag ccgcccgcct
       61 tgggcgtcga cttgagccac cccaggggct cgtcggcgtc gccgacgttc gacaggctga
      121 tgatggccgg caggatgagc agcgacacca ggttcatcac cttgatgagc gggttgaggg
      181 ccggaccggc ggtgtccttg aacgggtcgc cgacggtgtc ggcgatcacg gcggccttgt
      241 gggcgtcgga gcccttgccg ccctcggcgc cgtcctcgat gtacttcttg gcgttgtccc
      301 aggccccacc agcgttgttc tggtagttgg ccatgagctg gccggtcagg atgaccgcgg
      361 ccaggaaggc gcccaaggcg tagaagttca cgccgaagcc gatgatcacc ggcgtcagca
      421 cggcgagcag ggccggggtg gccagctccc gcagtgaagc ggtcgtgcag atgtcgatga
      481 ccgggccgta gtcggggcgc ttctcgccgc tcatgatctt gccgtcggcg aactgccggc
      541 ggacctcctg gaccacgacg gcggcggtgc ggccgacggc gcggatggcc agggagctga
      601 acatgaaggc gatggagcca ccgatgagga ggccgatgaa cgtcttcggg tcggccacgt
      661 tgatcgggat ggcctcgaag aggttctggc ccggccccac gaggtcgggc agctcgtcgg
      721 ccaccgtctc gatgtagctg gcgaacaggg ccacggcggc gatgaccgcc gacccgatgg
      781 cgaagccctt ggtgacggcc ttggtggtgt tccccacggc gtcgaggctg accatgatcc
      841 gctcggcctc acccgagaac tcaccggaca tctcggcgat gccggcggcg ttgtcggcca
      901 ccggaccgaa cgtgtcctcg gacacgacca cgccggtggt ggccagcatg cccatgccgg
      961 ccagagcgac caggtagaag acgaactgga tgttcccccc accgaggccg atgacggccc
     1021 cgagggcgac ggcgatggcc acgatcgccc acaccgagga ctcgaggccg gagctgatgc
     1081 cggagagcac ggtcgtggcg gggcccgtgc gggccgcctc ggcgatgtcc cggaccggcg
     1141 tgtgctcggt cgacgtgaag tactcggtga ggcggctgac gacctgggcg agtatgagcc
     1201 cgatcacgac tgcaccgagc atgcgccaac ccgggttgct cacgtacccg gccgcgctgg
     1261 ggtcaccggg atcgttgccg acgtagacga gggcgacgat gccggtgccg atgatcgtca
     1321 ggatcgtggc ggtgaggaag ccccggttga tgggggccat cgccgacttg tccttctcgg
     1381 tggcccggac ggagaagacg ccggcgatgg aagccagcac gccgatggcg cgagcggcca
     1441 ccgggaagat catgccgacg acggggttga gctcgccgat cgactcgaag gcggcgacgc
     1501 cgaggatgat cgaggccacc agggtgacct cgtagctctc gaagaggtcc gaagccatgc
     1561 cggcgcagtc gcccacgttg tccccgacgt tgtcggcgat ggtggccggg ttgcgggggt
     1621 cgtcctcggg gatgcccgcc tcgaccttgc cgaccaggtc ggcgcccacg tcggcggcct
     1681 tcgtgaagat gccaccgccg acgcgcagga agagggcgag gagcgaccca ccgaagccga
     1741 agccgatcag gatggccgag ctggtgttct ggaagatcat gatgatgacc gtggcgccga
     1801 gcaggcccag gccgacggtg aacatgccgg ccacgccgcc ggtgcggaac gccacctcga
     1861 gggcttcggg catggagccg ctacgggcgg cggcggcggt gcgcacgttg ccccggacgg
     1921 ccaggctcat cccgatgaag ccggtgaggc cggacaggaa gcagccggcg aggaaggcca
     1981 gggtccggaa gagaccggac tcgaactggc tgagcccgct gacgtaaccc agggcgtcgg
     2041 tcggcttcac cacctcggtc gaggtgatga agacgatgat cgccagcggg atcacgatga
     2101 tcgcgatcgt cttgaactgg cgcttcaggt aggccatcgc gccttcctgg atcgccgccg
     2161 cgatctcccg catcttgtcg gtacccgtgt cggcggcgtt gaccgcacgc accaggtaga
     2221 agccgacggc gagggcgatg agtgcggtga cggcggagaa gatcagccag aaccactcac
     2281 ctccgccgag ctcgaaggat tgatagccgc cttcagcggc caaaagggca gacatcgtcg
     2341 tcgaagtcct ttcggagcgg gccgggtccc tcccggccca cagggtccga aacggtagtc
     2401 gggggcctcc accgcgacag aatcggcgcg tccggccgat gggaaccggt catggctcga
     2461 gcgacccgat gtggcctcat tgaccacatc agccccacca gcaaccgtcc accacgctcc
     2521 gtcgcaccac gcgcccggct tgacacggag ggtgtcgagg gctttcctat tacggttcag
     2581 ttacaacttt gccgaaagaa ccacccgttg ccttcaccgt ccttccgctc tctgacccgc
     2641 ggcgtcctcg ccgttgtcgc cgcgacgacg atggtcgtcg tcccggttct ggtccaggcc
     2701 gaggagtccg acgacccccg agccgagcgg gaccgggtcc gcgagcaggc cgcggacgtg
     2761 gccgctcagc tcgacgggct ccaggccgac gcccaggagg tcggtgacgc cctccgggcc
     2821 ctggccgaca acgtgcagag caagcgggcc cagttccagg acgcccaacg ggccgcggcc
     2881 acggcactgg ccgaggcccg ggccgcccgc gcggaggagc agcgcctcca ggaccagatc
     2941 gccgacctcg aggaccaggt cgcccagatc gccgtcgagg cctacatccg cccgcccgag
     3001 agcaacgacg ccgccgtcta cgagacctcc gacatcaacg acaccacgct gcgggccgcg
     3061 ctgttgggaa ctcagtccaa ccgccaggag gacgtgatcg accagctccg ggccgcggag
     3121 gacgacctct ccgcccagcg cgtcgcggcc gaggccgcgg cccaggaggc ccgcgccctc
     3181 cgcggccaga cccggaccca gctcctcgag ctgcgagcgg cccagaacca gcagcgggag
     3241 ctggcccgtc aggtcgatga gcgcatcaac caggccgccg gcgaggccgc cgccctcgcc
     3301 gagcgcgacg aggagctgtc ggaccagatc gccgaggagg ccgcccagtt cgcctccttc
     3361 atccagggct tgaacctcgg cccggtcggc ccgtcgtacc agagcggtcc cgtctcggtc
     3421 gccgatgccg gcaacggcat catcgtgaac gtggcgatcg tggaccaggt ccacaacatg
     3481 atcgtggccg cggccaacga cggggtgacc ctgaccggcg gtggctaccg ggactcggct
     3541 cagcagatcg ccctgcgtcg ggcccactgt ggctccagcg actacgccat ctaccaggcg
     3601 ccgtcgtcgt cgtgctcgcc gccgacggcc atccccggca cgtcgatgca cgagcagggc
     3661 ctggccatcg acttcgacaa ctgctcctac ggcacgtcgg tctacaactg gctgtccggc
     3721 cacgctgcgg gctacgggct ctacaacctg ccgtcggagt cctggcactg gtccaccacc
     3781 gggggctagc cgtcggccgg tgacccgacc cagactgggc cggtggacct caccgttgcg
     3841 accgaaaccg tcgacgggat ctgggaggac agcgctctcg ccgcgctgac cgactacatc
     3901 cgcatcccgg ccaagtcacc ggcgtacgac gccgactggg tcgcccacgg ccatctcgcg
     3961 gatgccgtcg aactggtcgc agcgtggtgt cggagccgag acctccctgg cgccaccgtc
     4021 gagatccggg agctgcccgg gcgcacgccg gtcgtcctgg tcgagg
//
LOCUS       DASTFV010000353         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1298653, whole genome shotgun sequence.
ACCESSION   DASTFV010000353 DASTFV010000000
VERSION     DASTFV010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298653"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VFP02_06550"
     CDS             complement(<1..620)
                     /locus_tag="VFP02_06550"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9542721.1"
                     /translation="MSNALDSARDLYSTIAANAERTSPEPVPTETVEALTEAGLHAIA
                     TPREVGGSELALVDQMDVLAEVARADGSAGWCLLANSSTIAFFGAWAGDGFAEKLFAD
                     GVPLAAGQFAPNGVAVPEDGGYRLTGDYQFGSGIAHSSWAGAGTITEEPEGTDPRFLF
                     ALMPVEDIELRGNWDVLGLQSTASYDYAVRDVHVTEEATFDFFAPVR"
     gene            644..>2102
                     /locus_tag="VFP02_06555"
     CDS             644..>2102
                     /locus_tag="VFP02_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9542722.1"
                     /translation="MSTITTACPLDCPDGCSLSITVEDEKITAIDAVPEGEAANPLTD
                     GWICRKVRRMTRRIHGDQRLTTPLRRTGPKGSGQFEPIGWDEALDEIAAHIRTAIDEH
                     GPSSVVPYLYNSSAAVLESDGLTPLLFEALETAEVEHTICAATASKAWRLVLGTMPSA
                     DPTDVADSDLIVVWGANPTVSNSHLAPRIEARAREGAKVVVIDPRRTPAADRASRFLP
                     VKPGTDVVLAMAAAAELERRGAVDRAFVDEHAEGADEYLAACAEWTVERAAEVCGLEP
                     EDITGFVDDIVGAQAPMLRVGWGMERNRNGGSAHRATMSLWVLAGAFGRPGAGVVGAT
                     SPAEDPTAGAVRNAVLGGGDYPNRRIVNMNRFGAVLAGEGGAVDVLFVQGSNPAASAP
                     NQPAVHEGLARDDLFTVVHDQVMTDTARFADIVLPATTSFEVDDIVAGYGGYWVQDSP
                     AVIPPVGESRPNNQVAHGLAIRLGFESEAFDPSPAR"
BASE COUNT          298 a          745 c          772 g          287 t
ORIGIN      
        1 cggacggggg cgaagaagtc gaacgtggcc tcctcggtca cgtggacgtc gcggacggcg
       61 tagtcgtagc tggccgtcga ctggaggccg agtacgtccc agttgccccg tagctcgatg
      121 tcctcgacgg gcatgagggc gaacaggaag cgggggtcgg tgccctcggg ctcttcggtg
      181 atggtgcccg ccccggccca cgacgagtgg gcgataccgg agccgaactg gtagtcgccg
      241 gtaagtcggt agccgccgtc ctcggggaca gccacgccgt tgggcgcgaa ctggccggcc
      301 gccagcggca ctccgtcggc gaagagcttc tcggcgaagc cgtcaccggc ccacgcgccg
      361 aagaaggcga tggtcgacga gttggccagc aggcaccagc cggccgatcc gtcggcccgg
      421 gcgacctcgg cgaggacgtc catctggtcg accagggcca gctccgaccc gccgacctcc
      481 cggggcgtgg ctatggcgtg gagcccggct tcggtcaggg cctccacggt ctcggtcggt
      541 acgggctcgg gggacgtgcg ctcggcattg gcggcgatgg tgctgtagag gtctcgggcg
      601 gaatcgaggg cgttgctcac ggcgccctgt ctagccttca cccgtgagca cgatcaccac
      661 ggcctgtccg ctcgactgcc cggacgggtg ctcgctctcg atcaccgtgg aggacgagaa
      721 gatcacggct atcgacgccg tgcccgaggg ggaggcggcc aacccgctga ccgacggctg
      781 gatctgccgc aaggtccggc gtatgacccg gcgcatccac ggcgaccagc gcctgacgac
      841 cccgctgcgg cggaccgggc cgaagggctc gggccagttc gagccgatcg gctgggacga
      901 ggcgctggac gagatcgcgg cccacatccg caccgcgatc gacgagcacg ggccgtcgtc
      961 ggtcgtgccc tacctctaca actcctcggc cgcggttctg gagagtgacg gcctcacgcc
     1021 cttgctgttc gaagcgctgg aaacggccga ggtggagcac acgatctgtg ccgcgaccgc
     1081 ctccaaggcc tggcggctgg tgctggggac catgccgagt gccgacccca ccgacgtggc
     1141 cgacagcgac ctcatcgtgg tctggggcgc caatccgacc gtgtccaact cccacctcgc
     1201 tcctcgcatc gaggcccgag cccgggaggg ggccaaggtc gtggtcatcg acccccgccg
     1261 gacgcccgcc gcggaccggg cgagtcgctt cctgccggtg aagccgggga ccgacgtggt
     1321 cctggccatg gccgcggccg cggagctcga gcgacggggc gccgtggacc gggcgttcgt
     1381 cgacgagcac gccgaggggg ccgacgagta cctcgccgcc tgtgccgagt ggacggtcga
     1441 gcgagcggcc gaggtctgcg ggctggagcc cgaggacatc accggcttcg tcgacgacat
     1501 cgtcggggcc caggcgccga tgctgcgggt cggctggggc atggagcgca accgcaacgg
     1561 cggctcggcc caccgggcga ccatgtcgct gtgggtgctg gccggtgcct tcggtcgccc
     1621 gggtgcgggc gtcgtcgggg cgaccagccc ggccgaggac ccgaccgcag gcgccgtgcg
     1681 caacgccgtg ctcggcggcg gcgactaccc gaaccggcgc atcgtcaaca tgaaccgctt
     1741 cggcgccgtc ctggcggggg agggcggtgc ggtcgacgtc ctcttcgtcc agggctcgaa
     1801 cccggccgcg tcggccccca accagcccgc ggtccacgag ggcctggccc gggatgacct
     1861 gttcaccgtg gtccacgacc aggtgatgac cgacaccgcc cgcttcgccg acatcgtgct
     1921 gccggccacc accagcttcg aggtcgacga catcgtcgcc ggctatggcg gctactgggt
     1981 gcaggactcc ccggccgtga tcccgccggt cggcgagagc cggcccaaca accaggtggc
     2041 ccacggcctg gccattcgcc tcggcttcga gagtgaggcc ttcgacccca gcccggcgcg
     2101 cc
//
LOCUS       DASTFV010000354         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1300796, whole genome shotgun sequence.
ACCESSION   DASTFV010000354 DASTFV010000000
VERSION     DASTFV010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300796"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFP02_06560"
     CDS             complement(<1..323)
                     /locus_tag="VFP02_06560"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9542723.1"
                     /translation="MRAVVYHGPGLKAWEDVPKPEIIEDTDAIVRVDAVTICGTDLHI
                     LKGDVPAVTDGRILGHEAVGTVESVGTGVKNVAVGDHVLVSCITACGACRYCREGSYS
                     QCLGGG"
     gene            complement(428..1831)
                     /locus_tag="VFP02_06565"
     CDS             complement(428..1831)
                     /locus_tag="VFP02_06565"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9542724.1"
                     /translation="MRFDLSRRRFLQASAATAAATAFGARRLANVFGQGQVRPPGSRP
                     FPNLPAGTESMPEVEHIVIYMQENRSFDHYFGMLGRGDGFTLDVNGVPTNANPDLDGN
                     PVPVFPASSRCETTTTASQSWNDTHLSMNGGAMDGFVSAADGESGSMQYYDDATLPFY
                     WGLAETFPLCDRWFCSAPCQTFPNRRFLQAATSVGIVSTDVNEVLATPVAPNGVIWER
                     LNRHGISWNDYAIDLADILVFPTFGFAHQDRIKTFNDFLVDCARGTLPQVSIISPGTE
                     TYTEEAPADVQNGEAYSASIINAVLRSPTWERTVLFFTYDEHGGYYDHVPPPAAVKPD
                     DIAPRITVPPDEPGAFDVLGPRVPGFVISPFAKANYVSHVVHDHTSILKFIETKFNLG
                     AMTYRDANADDLLDCFDFANPGFLDPPALPEPGLPAAGSACQPLPRPQTEPDFGEPTP
                     PPTPATGPVGAAPPFTG"
BASE COUNT          297 a          646 c          793 g          338 t
ORIGIN      
        1 ccgccgccga ggcactggct gtagctgcct tcgcggcagt agcggcatgc cccgcaggca
       61 gtgatgcagg acacgagcac gtggtcgccg acggcgacgt tcttcacccc ggtgccgacg
      121 gactcgacgg tgccgacggc ctcgtggccc aggatgcggc cgtcggtgac ggcggggacg
      181 tcgcccttca ggatgtgcag gtccgtcccg cagatcgtta cggcatcgac ccgcacgatg
      241 gcgtcggtgt cctcgatgat ctccggcttg ggtacgtcct cccaggcctt cagaccgggg
      301 ccgtggtaca cgactgcacg catgatggct ctcctctgtc gcttcaactc cgagtctgcg
      361 gacccgcgcc cgcggggggt agagccatag gtcctgtcct cgagccggtg cggggggtgc
      421 ggtgggctca gccggtgaag ggtggggcgg ccccgacggg cccagtggcc ggcgtgggcg
      481 gaggcgtcgg ctcgccgaag tccggctcgg tctggggccg ggggaggggc tggcaggcgc
      541 tgcccgccgc cggcagtccg ggctcgggca aggcgggcgg gtcgaggaag ccggggttgg
      601 cgaagtcgaa gcagtcgagg aggtcgtcgg cgttggcgtc ccgataggtc atcgcgccga
      661 ggttgaactt ggtctcgatg aacttgagga tcgacgtgtg gtcgtggacc acgtgggaca
      721 cgtagttggc cttggcgaac ggggagatga cgaagcccgg gacccggggc ccgagcacgt
      781 cgaaggcgcc gggctcgtcc ggcggcaccg tgatgcgggg ggcgatgtcg tcgggcttga
      841 ccgcggccgg cggcgggacg tggtcgtagt acccgccgtg ctcgtcgtag gtgaagaaga
      901 gcaccgttcg ctcccacgtc gggctgcgca ggaccgcatt gatgatcgac gcgctgtagg
      961 cctcgccgtt ctgcacgtcg gcgggcgcct cctcggtgta ggtctcggtg ccggggctga
     1021 tgatgctgac ctgcggcagg gtccctcggg cgcagtcgac gaggaagtcg ttgaaggtct
     1081 tgatgcggtc ctggtgggcg aagccgaatg tggggaagac gaggatgtcg gcgagatcga
     1141 tggcgtagtc gttccaggag atgccgtggc ggttcaggcg ctcccagatc acgccgttgg
     1201 gcgccaccgg ggtggccagc acctcgttga cgtcggtgga cacgatcccg accgacgtgg
     1261 ccgcctgcag gaaccggcgg ttgggaaacg tctggcacgg cgccgagcag aaccaccggt
     1321 cacacaacgg gaaggtctcg gccaggcccc agtagaaggg gagggtggca tcgtcgtagt
     1381 actgcatcga gccgctctcg ccgtcggccg cggacacgaa gccgtccatc gccccaccgt
     1441 tcatcgagag gtgcgtgtcg ttccagctct ggctggcggt ggtcgtggtc tcacacctgc
     1501 tgctggccgg gaacaccgga accgggttcc cgtccaggtc cgggttcgcg ttcgtaggga
     1561 ccccgttgac gtcgagcgtg aagccgtcgc cccgtccgag catgccgaag tagtggtcga
     1621 aggaccggtt ctcctgcatg tagatgacga tgtgctcgac ctcgggcatc gactcggtcc
     1681 cggcgggcag gttggggaac ggcctactgc cggggggccg cacctggccc tgtccgaaga
     1741 cgttggccag ccggcgggcc ccgaaggccg tggccgcggc cgtggccgcg gaggcctgca
     1801 ggaaccgccg cctcgacagg tcgaatctca cccgctgcgc tcgatccacg tcgtcccctg
     1861 gcccttgctc cgcgagggcg aaccgtagac cttcggcgtc agggccgacg gctcctcaag
     1921 cgaagaggtc agtggcgggc tcgaggcggg gctgggcctg cacgaaccac tcgtggccca
     1981 tcatctcctc gagcagccat cgcgggaaca tgtcggggtc gatggggtac tgcgtgcggt
     2041 gggcggcgag agcggccatc ttccggtcga tgac
//
LOCUS       DASTFV010000355         2624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1301376, whole genome shotgun sequence.
ACCESSION   DASTFV010000355 DASTFV010000000
VERSION     DASTFV010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301376"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VFP02_06570"
     CDS             complement(<1..716)
                     /locus_tag="VFP02_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542725.1"
                     /translation="MSTTSVHTRPSPVLGVVLMAMVVLLAGVALVTPGAAQGEPAPTT
                     VPSATTVAPPPTTAVPTTVPPTTVPDPAPTPVPAPSSEPTGVRDVLLVGDSIMRSTGP
                     ALAHQLGARWRVHNEGVNGSGLLTPGVFPWPRHLAASLNRFDPDVVVMLFIGNYTADP
                     AQKWIGADGRRVPDIYARSFAREWGRQAERFVTQMIPSGADVVLVLPPPMISARVQIV
                     IDRLRAEYRAVASRHPEVTLA"
     gene            992..1888
                     /locus_tag="VFP02_06575"
     CDS             992..1888
                     /locus_tag="VFP02_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9542726.1"
                     /translation="MITEPGRTPLHLVVRRPVDVGRDCDGLLLADAELSRQHLRLSAT
                     AGRLVVADLGSTNGTKLDGAELRGPTRVAPGQVVTFGHCRLELVAPVNGRAASIAADP
                     LRRTSIDVVADAAVADPMPATPFGGGTLTIVFSDIEQSTRRTEELGDAAWLGLLGLHN
                     SVVRRQLERHGGHEVKAQGDGFMLAFPSARAAVLFAIGVQRTLDAHGRSQPAELLRVR
                     IGMHTGEAIMEDGDLFGHSVVLAARIAGRARGGEILVSSLVREIVESRGDLSFGETRK
                     AELKGLAGTHQLHPILWAAGNG"
     gene            1899..>2624
                     /locus_tag="VFP02_06580"
     CDS             1899..>2624
                     /locus_tag="VFP02_06580"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HET9542727.1"
                     /translation="MEGEKVDLRFSVLGGTIGVSEAGHPVALGGAQQRRVLAAFLAEA
                     GVVVSADRLVEAVWPDGSAPEGARRTAMSYVSRLRAAIGAEHLVTRDNGYVLELAGAS
                     YDAAEFEARLAEARQLLGEEAVLSYDAALALWSGRAFGDDGSEWWLRPVAARLDELRL
                     LAEEERAERLIDCGRHAEAVVDLDRLVTEQPLRERFVDLLMRALYLAGRQAEALRAFQ
                     RFRDYLAEETGLPPPDALVDLEQR"
BASE COUNT          353 a          897 c         1003 g          371 t
ORIGIN      
        1 gccagggtca cctcggggtg acgggaggcc accgcccggt actcggcccg gagacggtcg
       61 atcacgatct ggacccgggc gctgatcatc ggtgggggca gcaccaggac gacatcggcg
      121 ccggagggga tcatctgggt cacgaaccgc tccgcctggc ggccccactc ccgggcgaag
      181 gaccgtgcgt agatgtcggg cacccgacga ccgtcggcgc cgatccattt ctgggcgggg
      241 tcagcggtgt agttgccgat gaagagcatg accacgacgt cggggtcgaa ccggttcagc
      301 gaggcggcga ggtgcctggg ccaggggaac acgccgggcg tgagcaggcc ggagccgttg
      361 actccctcgt tgtggacccg ccagcgggcc ccgagctggt gggccaacgc cgggccggtc
      421 gacctcatga tcgagtcacc gaccaggagg acgtcccgca ccccggtcgg ctcactgctc
      481 ggcgcaggaa ccggcgtcgg agccggatcg gggaccgtcg tcgggggcac cgtggtcggg
      541 acagcggtgg tcggcggagg ggcgaccgtc gtcgccgacg ggacggtggt cggggccggc
      601 tcgccctggg cggcgcccgg ggtcacgagg gccacccctg ccaggaggac caccatggcc
      661 atgaggacca cgccgaggac cggggacggg cgcgtgtgaa cagaggtggt ggacatggct
      721 tcctcctggt ctcggtgacc atcgagccac cgggccgatc agcgggctct caccccccgg
      781 taccctcggc cccggggtgt caccgggtcg tccggcaaca cctgcggatc atcacaagag
      841 gcgtttgcag ggggtcgacg aaccgtcgac aaaccgtcga catgacgggc gtcacacccg
      901 ccacgcgaag ccggcggagg gggactgtcg aagttctgca gacgccgaga gtgagactgg
      961 ccgagtggag gcgaggccgt gtccgaccgt catgatcacc gagccggggc gcacgccgct
     1021 gcacctggtg gtccgccggc cggtcgacgt cggtcgtgac tgcgacggcc tcctcctcgc
     1081 agatgccgaa ctctcccgtc aacacctgcg gctgagcgcc acggccggcc gcttggtggt
     1141 agccgacctc ggcagcacca acgggacgaa gctggacggc gccgaactga ggggaccgac
     1201 ccgggtggcc cccggtcagg tggtcacctt cgggcactgt cgcctggagc tggtcgcccc
     1261 ggtgaacggc cgggcggcct ccatcgccgc tgaccccttg cgccgcacct cgatcgacgt
     1321 cgtggccgac gccgcggtcg ccgacccgat gcccgccacg cccttcgggg gcgggacgtt
     1381 gacgatcgtg ttctccgaca tcgagcagtc gacccgccgc accgaggagc tgggtgatgc
     1441 cgcctggctc gggctgctgg gtctgcacaa cagcgtcgtc cgccgccagc tcgagcgcca
     1501 tggcggccac gaggtcaagg cccagggcga cggcttcatg ctggcgttcc ccagtgcgcg
     1561 ggccgccgtg ctgttcgcca tcggcgtcca gcgcaccctc gacgctcacg gccggtccca
     1621 gcccgcggaa ctgttgcggg tgcggatcgg tatgcacacc ggcgaggcga tcatggagga
     1681 cggcgacctc ttcgggcact ccgttgtctt ggccgcccgg atcgccggcc gggcccgggg
     1741 tggagagatc ctggtgtcgt cgctggtgcg ggagatcgtc gagtcccgcg gggacctgtc
     1801 tttcggcgag acgcggaagg ccgagctgaa gggcctggcc ggcacccacc agctgcaccc
     1861 gatcctctgg gcggctggga atggttgata ctcgcgcggt ggagggggag aaggtcgacc
     1921 tgcggttcag cgtgctcggg ggcaccatcg gtgtgtccga ggccggtcac ccggtcgcct
     1981 tggggggtgc gcagcagcgc cgggtgctgg ccgcgttcct ggccgaggct ggggtggtgg
     2041 tcagcgcgga ccgtctggtg gaggcggtct ggcccgacgg ctccgccccc gagggtgccc
     2101 gtcgcaccgc catgtcctac gtgtcccgcc tgcgggccgc gatcggggcg gagcacctgg
     2161 tcacccggga caacggctac gtcctggagc tggccggggc gtcctatgac gccgccgagt
     2221 tcgaggctcg cctcgcggag gcccgccagc tcctcggcga ggaggcggtc ctctcgtacg
     2281 acgccgcgct cgccctgtgg tcggggcgag ccttcggtga cgacgggagc gagtggtggc
     2341 tgcggccggt ggccgcccgc ctggacgagc tccgcctcct ggccgaggag gagcgggccg
     2401 agcgcctgat cgactgcggc cgtcacgccg aggccgtggt cgacctcgat cggttggtca
     2461 ccgagcagcc gctgcgggaa cggttcgtcg acctgctgat gcgggccctg tacctggcgg
     2521 gccggcaggc cgaggcgctg cgggccttcc agcgcttccg ggactacctg gccgaggaga
     2581 cgggcctgcc cccgccggat gcgctggtcg acctggagca gcgc
//
LOCUS       DASTFV010000356         4498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1305389, whole genome shotgun sequence.
ACCESSION   DASTFV010000356 DASTFV010000000
VERSION     DASTFV010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305389"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFP02_06585"
     CDS             <1..485
                     /locus_tag="VFP02_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542728.1"
                     /translation="TTVASDGDAVTALSPRQLSCTDEPCLVDFGINDPPAGTSPAVFV
                     QFTADPLRIDVIGRGLGAPEDWVRQGSAAHRVGATRFGVFLSARDDDPFGGGGLVCSW
                     AEGEDGCTGTTIPRYAPSYNRLSVLAIVDETTEPSLSDGADRLVRTLSFDPPLRAVEP
                     "
     gene            502..1737
                     /locus_tag="VFP02_06590"
     CDS             502..1737
                     /locus_tag="VFP02_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542729.1"
                     /translation="MTGKTMRRWTGMLLALVLLAAACSDDDGEDAPAPPDDTEVASGD
                     PTTTTVVDDGLPDDVIVFNGQGNNLDAYSTGETVETQRVISTIADDPDGLDINAQICF
                     FPDGSGRFIAGEDTGQPTPPAGWGIFQLSGTGVGDLEAEEIGKLTPTYQAGADPENYG
                     CGVLSDGRMLTTDLGGQAPGDPGNGQFIVWFGEPEGTDQTYCKVDVELATAQSLLVGP
                     DDEVYVVSARPPTSGVWRYTDLPTSADAAGGCGSTDVTGAPMADAVTREQVLAPGQND
                     ILSPAGIAFSPNGGFYLSSVITGVINEYDADWQFVRTILAPPEGEAIGADASLSTGTP
                     LGIGVTPDGQVWYADIGIVNDPENGFGPGDGTGHVRRILFVDGEPQPPETVASDLAFP
                     DGIGVYVPGGAAGGGANPA"
     gene            complement(1759..1914)
                     /locus_tag="VFP02_06595"
     CDS             complement(1759..1914)
                     /locus_tag="VFP02_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542730.1"
                     /translation="MTTIPDPGPDGPKEPAPVIPDPDPNPEPAPIIPDPNPNEEPAPI
                     IPDPNPV"
     gene            2212..3123
                     /locus_tag="VFP02_06600"
     CDS             2212..3123
                     /locus_tag="VFP02_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9542731.1"
                     /translation="MSLAPVAGSRGGFEVAVHGGDLAMGGSVVGPDGPVVGATVLLER
                     FVGQSSGRLEVRTNAEGRWVAIDVYGGRYRIRAWRSPGLGMASSVVSFIAADAEVDLA
                     LTVDRYDGADVTGDVDDADPEVGATAVVTALATRQQVDGQGIITTAPASGRDALVTPT
                     GPWTLTGPPEAVIDDAGRVSWTFTCAAAGPVTALVEALGTETTVSATCVPPPVVLPPP
                     VDTPEPDFPIGGSFTPPFDGPVPAGTYTIIDDPGTCVLTYEAWTGSAWDPARRTVTGT
                     GVVSIPDIARNLEALGDSPACTYERTT"
     gene            3120..>4498
                     /locus_tag="VFP02_06605"
     CDS             3120..>4498
                     /locus_tag="VFP02_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET9542732.1"
                     /translation="MKPAKRRRRWWAIPRMTVKIVVLTAFFAAIAVPAGAAAGFWALL
                     NADLPGNLPEDTNPRIRSYPSLVVDADGNEIAEFRAFELTVPIRYRDIPQGVKDAVVA
                     IEDQRFWEHKGVDPEGLVRAALVNFEHGEVVQGGSTITQQYVKNTYTTGERDVARKLR
                     EALIATRLERQLTKDEILFRYLNTTYFGDGAYGIGAAAQSYFGKPVADLTLSEAALLA
                     GAIASPGRFGPRVNAETAERRRLAVLRAMRDQDLIGRHQFLGARRQHIWYAPLGPPPG
                     PATIVQPPPRGGASAYPYFVDYVRRVLEERYGHRQLYRGGLRIETTLDPGLQAQAEAS
                     VAETLSGTSAPLEMSLVSVEPATGHVRALVGGRDFDGSQVNLALGGSAGMQPGSSFKT
                     FVLATALEHGIDPETTFDAPASMDFSGCDPSCTINNYDFEDHGSLTLRAATHQSINTV
                     YAQLIDRLGP"
BASE COUNT          613 a         1715 c         1532 g          638 t
ORIGIN      
        1 cgacgaccgt ggcgtccgac ggcgatgccg tgaccgcgct gtcaccgcgg cagctgtcct
       61 gcaccgacga gccctgcctg gtcgacttcg ggatcaacga tcctccggcg ggcacgagtc
      121 ccgcggtctt cgtccagttc accgccgacc ccctgcgcat cgacgtcatc ggtcggggcc
      181 tcggtgctcc cgaggactgg gtccgccagg gctcggcggc ccatcgcgta ggggccactc
      241 gcttcggggt cttcctctcc gcgcgcgacg acgatccgtt cggcggtggg ggcctggtct
      301 gcagctgggc cgagggggag gacggctgca ccggcaccac catcccccgc tacgcgccct
      361 cgtacaaccg gctgagcgtg ctcgccatcg tggacgagac gaccgagccg agcctgtccg
      421 acggggctga tcgcctcgtg cggacgctga gcttcgatcc gcccctccgg gcggtggaac
      481 cgtgacaaga gaggaaccgc catgaccggc aagaccatga ggcgatggac ggggatgctc
      541 ctggcgctgg tgctgttggc cgcggcctgc agcgacgacg acggggagga cgcgccggcc
      601 ccaccggacg acaccgaggt cgcgtccggc gacccgacga ccacgacggt ggtcgacgac
      661 ggcctgcccg acgacgtgat cgtgttcaac ggccagggca acaacctgga cgcctacagc
      721 acgggcgaga cggtcgagac ccagcgggtg atcagcacca tcgccgacga cccggacggc
      781 ctcgacatca acgcccagat ctgcttcttc cccgacggct cgggccggtt catcgccggc
      841 gaggacacgg gccagcccac gcccccggcg ggctggggca tcttccagct ctcgggcacc
      901 ggcgtgggtg acctcgaggc ggaggagatc ggcaagctga cgccgaccta ccaggccggc
      961 gccgatcccg agaactacgg ctgcggcgtg ttgtccgacg ggcgcatgct gaccaccgac
     1021 ctcggcggcc aggcccctgg cgacccgggc aacgggcagt tcatcgtctg gttcggcgag
     1081 cccgagggca cggaccagac ctactgcaag gtcgatgtcg agctggccac ggcccagagc
     1141 ctcctggtcg gccccgacga cgaggtctac gtcgtctcgg cccgtccacc cacctccggg
     1201 gtctggcgct acaccgacct acccacctcc gccgatgcgg ccggtggctg cgggagcacc
     1261 gacgtcacgg gggcgcccat ggccgacgcc gtcacccggg agcaggtgct ggccccgggg
     1321 cagaacgaca tcctgtcgcc cgccggcatc gccttcagcc cgaacggggg gttctacctg
     1381 tccagcgtga tcaccggcgt gatcaacgag tacgacgccg actggcagtt cgtgcgcacc
     1441 atcctggccc cgcccgaggg ggaggccatc ggggcggacg cgtcgctgtc gacgggcacc
     1501 ccgctgggca tcggggtcac ccccgacggc caggtctggt acgccgacat cggcatcgtc
     1561 aacgaccctg aaaacggctt cggcccgggt gacggcaccg gtcacgtccg ccgcatcctg
     1621 ttcgtcgacg gtgagcccca gccccccgag accgtcgcct cggacctggc cttccccgac
     1681 ggcatcgggg tctacgtccc cggcggcgcc gccggcggag gcgccaaccc cgcctgaccc
     1741 cgaacgttca ggccacgatc agaccgggtt ggggtccggg atgatcgggg ccggctcctc
     1801 gttggggttc gggtcgggga tgatcggggc gggctccggg ttggggtccg gatcggggat
     1861 gaccggcgcc ggctccttcg gcccgtcggg cccggggtca gggatggtcg tcatgggcgt
     1921 tctcctcgtc gcggtcaccg cgtggctacc cgctcggacc cggggccatt cgccgccgcg
     1981 ggcctgtatt gccagccgca tctgccgatg gatcaggcga tggcccagcc gccgaagact
     2041 ggggcttcga cccgacgcgc cggacgcgtc gcgtcggttg ccgcgtgcat cgctctcctc
     2101 ggcgcgctcg gcgccggttg ctcctctccc cccgaggagg cggccccacc gacctcggct
     2161 ccccgccagc ggccgatcgt cgcccccgag cccctgcccg acacccggtc cgtgtccctg
     2221 gccccggtgg ccgggtcgcg gggtgggttc gaggtcgccg tccacggtgg cgacctcgcc
     2281 atgggcggct cggtcgtcgg tcccgatggc cccgtggtcg gggccaccgt gctcctggag
     2341 cgcttcgtcg gtcagagctc gggtcggctc gaggtccgga ccaatgccga gggccgctgg
     2401 gtcgccatcg acgtgtacgg cggtcgctac cggatccggg cctggcgcag cccgggcctg
     2461 gggatggcgt cctcggtcgt ctcgttcatc gccgccgacg ccgaggtcga cctggcgctg
     2521 accgtcgatc gctacgacgg tgcggacgtc accggcgacg tcgacgacgc cgatcccgag
     2581 gtcggggcca ccgccgtcgt caccgccctg gccacccgcc aacaggtcga cggccagggc
     2641 atcatcacca ccgcgccggc gtccgggcgg gacgcactcg tcacgcccac cgggccctgg
     2701 accctgaccg gccccccgga ggcggtcatc gacgacgccg gtcgggtgag ctggacgttc
     2761 acctgcgccg cggcgggccc ggtcaccgcg ttggtcgagg ccctcggcac ggagaccacg
     2821 gtgtccgcca cctgcgtgcc cccgcccgtc gtcctgcccc cgccggtgga cacacccgag
     2881 ccggacttcc cgatcggggg ttcgttcacc ccaccgttcg acgggcccgt gcccgccggc
     2941 acgtacacga tcatcgatga tcccggcacc tgcgtcctga cctacgaggc ctggacgggt
     3001 tccgcctggg acccggcccg ccggacggtc accggtaccg gcgtcgtgtc gatcccggac
     3061 atcgcccgga acctcgaagc gctgggtgac tccccggcct gcacctacga gcgcacgaca
     3121 tgaagccggc caagcgtcgt cgccgctggt gggccattcc ccgcatgacg gtgaagatcg
     3181 tcgtgctgac ggccttcttc gcggcgatcg ccgtccccgc cggcgccgcc gccgggttct
     3241 gggccctgct gaacgccgac ctccccggca acctccccga ggacaccaac ccccggatcc
     3301 gctcctaccc cagcctggtg gtggacgccg acggcaacga gatcgccgag ttccgggcct
     3361 tcgaactgac cgtgcccatc cgctaccggg acatccccca gggggtgaag gacgctgtcg
     3421 tcgccatcga ggaccagcgc ttctgggagc acaagggcgt cgaccccgag ggcctggtcc
     3481 gggccgcgct ggtcaacttc gagcacggcg aggtcgtcca gggcggctcg accatcaccc
     3541 agcagtacgt caagaacacc tacacgaccg gtgagcgcga cgtcgcccgc aagctgcggg
     3601 aggcgctgat cgccacccgg ctcgaacggc agctgaccaa ggacgagatc ctcttccgtt
     3661 acctgaacac gacctacttc ggtgacgggg cctacggcat cggcgccgcc gcccagtcgt
     3721 acttcggcaa gccggtcgcc gacctcaccc tgtccgaggc cgcgctcctg gcgggcgcca
     3781 tcgcctcacc cgggcgcttc ggcccccggg tcaacgccga gacggccgag cgccggcggc
     3841 tcgccgtcct ccgggcgatg cgggaccagg acctgatcgg ccggcaccag ttcctgggcg
     3901 cccgccgtca gcacatctgg tacgccccgc tcggcccccc gccgggtccg gcgacgatcg
     3961 tccaaccccc gcctcggggc ggggcgtcgg cctaccccta cttcgtggac tacgtccgtc
     4021 gggtgctgga ggagcgctac ggccaccgtc agctctaccg gggtggcctg cgcatcgaga
     4081 ccaccctcga tcccggcctg caggcccagg ccgaggcgtc ggtggccgag acgctgagtg
     4141 ggaccagtgc cccgctggag atgtccctgg tgtcggtcga gccggccacc ggccatgtcc
     4201 gcgccctcgt cggtgggcgg gacttcgacg gcagccaggt caacctcgcc ctcggcggct
     4261 ccgcgggcat gcagcccggc tcgtcgttca agaccttcgt gctggccacg gccctggagc
     4321 acgggatcga cccggagacg acgttcgacg ctccggcgtc catggacttc tccggctgtg
     4381 acccgagttg cacgatcaac aactacgact tcgaggacca cgggtccctg accctccgcg
     4441 ccgccaccca tcagtcgatc aacaccgtct acgcccagct catcgaccgg ctcggtcc
//
LOCUS       DASTFV010000357         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1306657, whole genome shotgun sequence.
ACCESSION   DASTFV010000357 DASTFV010000000
VERSION     DASTFV010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306657"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1521)
                     /locus_tag="VFP02_06610"
     CDS             complement(121..1521)
                     /locus_tag="VFP02_06610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9542733.1"
                     /translation="MIAPMTAKDERSVSWWMETGGDDLTPRPPLDGSTSADVAILGAG
                     YTGLWTALYLLERDPSLNVVLVEREIAGWGASGRNGGWVSPLISVSHTEMIARHGKEA
                     TRATHDALIDTIGHVESAVERHGIDAHLDRHGMLMLARGGHQMTALRHSYDEMAELGD
                     PDFYRWMEPGEAAELVDVDNTWAALFAEPFAAVHPGRLVRGLARAVEGLGATIYEGTA
                     VTGYDLTTKPVLHTTGGDVSADVIVLAGEAYLSQLPKHGREILPVYSQIMLTAPLSEA
                     QRATIGWRRRFVMQSKRLTIDYLQITRDNRIAFGGRGSLYHWGSAIKPEWDCVEATAH
                     SLRSQFDEFFPSLAEVPFERCWGGVVGIHRDWMPSFGFDKRTGLAKAGGYAGDGVAAT
                     NLAGRTLADLITGGGTPLCELPMVQHEPKAWEPEPLRYLGVRYIQKAAARMEEKAVRT
                     GKAPSGRSIAERLMSH"
     gene            1543..>2294
                     /locus_tag="VFP02_06615"
     CDS             1543..>2294
                     /locus_tag="VFP02_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542734.1"
                     /translation="MAPMRPDEPVLVGVGQHVHRPADGPVPSLIEMMTTAARRAGGDV
                     GPGVLDRLGWIGVPKGAWTHPDPGREVAAAVGAPDAHTVLVEVGITQQEVIDLALAAV
                     RDGAGPALVVGAEVTHGSTAGAATAVGTARRAAVEPDEHLIAHDLGVSAVEMNQLFID
                     PPTVYAVLENAFAAAQGWDEVQHRRRLGDLTEAFAGIAAWNSYAWQRDAPSAEDIVEP
                     STANRMIAEPYTKLCCSNLRVNQAAALLFTTV"
BASE COUNT          315 a          816 c          825 g          338 t
ORIGIN      
        1 gtcatcgacg cgctcgtggc ctcggccacc aagtactccg cggtctacga catgctgacg
       61 agccccacgt cggtgaccgt cacgcgagcc tgacccggga ccgcggggtc cgccccgggg
      121 tcagtgggac atcaggcgtt cggcgatgct ccggccgctc ggggctttgc cggtgcggac
      181 ggccttctcc tccatgcggg ccgccgcctt ctggatgtag cggaccccga ggtagcgcaa
      241 gggttcgggc tcccaggcct tgggctcgtg ctggaccatg ggtagctcgc agagaggtgt
      301 gcccccgccg gtgatcaggt cggccagggt ccggcccgcc aggttggtgg cggccacccc
      361 gtccccggcg tagcccccgg ccttggcgag gcccgtccgc ttgtcgaagc cgaacgacgg
      421 catccagtcc cggtggatgc cgaccacacc accccagcac cgctcgaagg ggacctcggc
      481 cagagacggg aagaactcgt cgaactggct gcgcaggctg tgcgcggttg cctccacgca
      541 gtcccactcg ggcttgatgg ccgagcccca gtggtagagc gagccccggc cgccgaaggc
      601 gatgcggttg tcccgcgtga tctgcaggta gtcgatggtg aggcgcttgg actgcatgac
      661 gaagcgacga cgccagccga tcgtggcccg ctgcgcctcg gacagtggcg cggtgagcat
      721 gatctgggag tagacgggga ggatctcgcg gccgtgcttc gggagctggg agaggtaggc
      781 ctcccccgcc aggacgatga cgtcggcgct gacatcgccg cccgtcgtgt gcaggacggg
      841 tttggtggtc aggtcgtagc cggtgacggc ggtgccctcg tagatggtgg ccccgaggcc
      901 ttcgacggcc cgggccaggc cccgtaccag gcgaccgggg tgcacggcgg cgaagggctc
      961 ggcgaacagg gccgcccacg tgttgtcgac gtcgaccagc tcggcggcct cgcccggctc
     1021 catccagcgg tagaagtccg ggtccccgag ctcggccatc tcgtcgtagc tgtggcggag
     1081 cgccgtcatc tggtgaccac cgcgggccag catcagcatc ccgtgacggt cgaggtgggc
     1141 gtcgatgccg tgacgctcca cggcggactc gacgtgcccg atggtgtcga tcagggcgtc
     1201 gtgggtggcc cgggtcgcct ccttgccgtg gcgggcgatc atctcggtgt ggctgaccga
     1261 gatcagcggc gacacccagc cgccgttgcg tcctgaggcg ccccagcccg cgatctcccg
     1321 ctcgacgagc acgacgttca ggctcgggtc ccgttccagc aggtagagcg ccgtccacag
     1381 tcccgtgtag cccgcaccca ggatggccac gtcggcgctg gtcgagccgt ccagcggcgg
     1441 gcgcggggtc aggtcgtcac caccggtctc catccaccag ctgaccgagc gctcgtcctt
     1501 cgctgtcatg ggggcgatca tggcaccact accgtccggg ccatggcccc gatgcgccct
     1561 gacgagcccg tcctggtcgg cgtgggccag cacgtgcacc gccccgcgga cggtccggtg
     1621 ccgtccctca tcgagatgat gaccacggcc gcccggcgcg caggcggtga tgtagggccc
     1681 ggagtgctcg accgcctcgg ctggatcggc gtccccaaag gggcgtggac ccacccggat
     1741 ccgggccgcg aggtggcggc cgcggtcggc gcgcccgacg cccacaccgt cctcgtcgag
     1801 gtcggcatca cccagcagga ggtgatcgac ctcgccctcg ccgccgtgcg ggacggggcc
     1861 ggccccgccc tggtcgtggg ggccgaggtc actcacgggt cgacggccgg cgcggcgacc
     1921 gcggtcggta cggcgcggcg ggcggcggtc gagcccgacg agcacctgat cgcccatgat
     1981 ctgggcgtca gcgcagtaga gatgaatcag ctcttcatcg acccgcccac cgtctacgcc
     2041 gtcctggaga acgccttcgc cgcggcccag ggctgggacg aggtccagca ccggcgccgg
     2101 ctgggcgacc tgacggaggc gttcgccggg atcgcggcct ggaactcgta cgcctggcag
     2161 cgcgatgccc cgtcagccga ggacatcgtc gagccgtcga ccgccaaccg gatgatcgcc
     2221 gagccctaca cgaagctgtg ctgctccaac ctccgggtca atcaggccgc ggccctcctc
     2281 ttcaccacgg tcga
//
LOCUS       DASTFV010000358         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1313973, whole genome shotgun sequence.
ACCESSION   DASTFV010000358 DASTFV010000000
VERSION     DASTFV010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313973"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFP02_06620"
     CDS             <1..573
                     /locus_tag="VFP02_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542735.1"
                     /translation="VGYNLLLPRSSDWPEFQAVFDPAYPDPIDRVIVLELIQLLWDRG
                     ENSAYVQHLTADPYEGVPAKTVLLVEAFGDHQVANVSTAVLARTIGAAVTDPALEPGR
                     SDDVEPNWGITALDGFPATTSVLSVWDYGTPAPPTVNLPPTEPEYGEDPHGAGSSEPG
                     VLTQALTFLTTGEVIDVCNGVPCVGRQLDG"
     gene            591..1733
                     /locus_tag="VFP02_06625"
     CDS             591..1733
                     /locus_tag="VFP02_06625"
                     /inference="COORDINATES: protein motif:HMM:NF015254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HET9542736.1"
                     /translation="MFRSLRPLLLAMVALVLLAAACGGDDSSDEAAADTTAPTDDTTA
                     DGTTDYAAVAAAAEWEEHVPGGDCQCADGSEFSFWSREGDPEKVVLFFQGGGACFTAE
                     MCSFTDGTYTVQADGSQVTLGSAGVFDAANEANPLRDYSFVFVPYCTGDIHLGDAVHD
                     YGGGLTVNHVGYVNASAGLDYVVENFPDASEVFVTGSSAGGVPSPMFAGLASDALPDA
                     DIASLSDASGAYPSSPAINAAIGSLWGTFANVPDWPENEGLTPEDWGVPTLFVQAGLH
                     DPDIRFARYDAAYDETQQSFSDAAGFAGDDLKTTIEDNEAQIEAAGVPVASYISPGTV
                     HTILGDNAMYDLEVEGVPFIDWLTDFVAGDGVGDVVCVDCQNPADA"
     gene            complement(1755..>2107)
                     /locus_tag="VFP02_06630"
     CDS             complement(1755..>2107)
                     /locus_tag="VFP02_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542737.1"
                     /translation="VETIETGGEPGGVVLTADAVWVTDASRDQVLQIDPATNTVVGQI
                     PVGTDPGGMASTDDTVYVSNSGDDTVFAIDAATAEVTDSFETGRKPGGLAVTGTDLWV
                     ANTDADTVSRIPLS"
BASE COUNT          298 a          799 c          707 g          303 t
ORIGIN      
        1 gtcggctaca acctgctgct gccccgctcc agcgactggc ccgagttcca ggccgtcttc
       61 gacccggcct acccggaccc gatcgaccgg gtgatcgtgc tcgagctgat ccagctcctg
      121 tgggaccggg gcgagaacag cgcctacgtc cagcacctga ccgccgaccc ctacgagggg
      181 gtgccggcca agaccgtgct gctggtcgag gccttcggcg accaccaggt ggccaacgtc
      241 agcaccgccg tgctggcccg cacgatcggc gccgcggtca ccgacccggc cctcgagccc
      301 ggtcgcagcg acgacgtcga gcccaactgg gggatcaccg cgctcgacgg gttcccggcc
      361 acgacctcgg tcctgtcggt ctgggactac ggcacgccgg cgccgcccac ggtcaacctg
      421 ccgcccaccg agcccgagta cggcgaggac ccccacgggg cgggcagcag cgagcccggc
      481 gtcctgaccc aggccctgac cttcctcacc accggcgagg tcatcgacgt gtgcaacggc
      541 gtcccctgcg tgggccgcca gctcgacggt tgaggactac tgtcccggcc gtgttccgct
      601 ccctccgccc cctgctcctc gccatggtgg ccctcgtcct cctcgccgcc gcgtgcgggg
      661 gcgacgactc gagtgatgag gccgcggccg acaccaccgc gcccaccgac gacaccacgg
      721 ccgacggcac caccgattac gcagccgtgg ccgcggcggc ggagtgggag gagcacgtgc
      781 cgggcggcga ctgccagtgc gccgacggca gcgagttcag cttctggagc cgggaggggg
      841 accccgagaa ggtcgtcctc ttcttccagg ggggcggtgc ctgcttcacc gccgagatgt
      901 gctcgttcac cgacggtacc tacacggtcc aggccgacgg cagccaggtg accctgggtt
      961 ccgccggggt cttcgacgcc gccaatgagg cgaacccgct ccgggactat tccttcgtgt
     1021 tcgtgccgta ctgcaccggg gacatccacc tcggcgacgc ggtccacgac tacgggggcg
     1081 gcctgaccgt caaccacgtc ggctacgtca acgcctcggc cggcctggac tacgtcgtgg
     1141 agaacttccc ggacgcctcc gaggtgttcg tcaccgggtc cagcgccggc ggcgtgccct
     1201 cgccgatgtt cgccggcctg gcctccgacg ccctgcccga cgccgacatc gcctccctgt
     1261 ccgacgcctc cggcgcctac cccagctcgc ccgccatcaa cgccgccatc ggcagcctgt
     1321 ggggcacgtt cgccaacgtg cccgactggc ccgagaacga gggcctgacc ccggaggact
     1381 ggggcgtgcc caccctcttc gtccaggccg gcctgcacga ccccgacatc cgcttcgccc
     1441 gttacgacgc cgcctacgac gagacccagc agtcgttctc ggacgcggcc gggttcgcgg
     1501 gcgacgacct caagacgacc atcgaggaca acgaggccca gatcgaggcc gcgggcgtcc
     1561 ccgtcgccag ctacatctca cccggcactg tccacaccat cctcggcgac aacgccatgt
     1621 acgacctcga ggtggagggc gtgcccttca tcgactggct cactgacttc gtcgccggcg
     1681 acggggtcgg cgacgtggtc tgcgtcgatt gccagaaccc cgccgacgcc tgacccagga
     1741 ggccctcggc ggcctcagct gagcgggatg cgggagacgg tgtcggcgtc ggtgttggcc
     1801 acccagaggt cggtgccggt gacggccagg ccgccgggct tgcgccccgt ctcgaacgag
     1861 tcggtgacct ccgcggtggc ggcgtcgatg gcgaagacgg tgtcgtcccc ggagttcgac
     1921 acgtagacgg tgtcgtcggt ggacgccatg cccccggggt cggtgccgac cgggatctgg
     1981 ccgaccacgg tgttggtggc gggatcgatc tggaggacct ggtcccggga ggcgtcggta
     2041 acccacacgg cgtcggcggt caggacgaca ccgccgggct cgccccccgt ttcgatggtc
     2101 tcgacga
//
LOCUS       DASTFV010000359         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1322088, whole genome shotgun sequence.
ACCESSION   DASTFV010000359 DASTFV010000000
VERSION     DASTFV010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322088"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFP02_06635"
     CDS             <1..336
                     /locus_tag="VFP02_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HET9542738.1"
                     /translation="RGLGEAGKYEMIEQLAKLLKAAPGASRAIVDAGWVPYSYQVGQT
                     GKVVKPTVYIACGISGATQHLVGMKGSKNIIAINKDAEAPIFSVADLGIVGDVHKVLP
                     QVIEALQNR"
     gene            complement(619..1593)
                     /locus_tag="VFP02_06640"
     CDS             complement(619..1593)
                     /locus_tag="VFP02_06640"
                     /inference="COORDINATES: protein motif:HMM:NF039678.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6183 family protein"
                     /protein_id="HET9542739.1"
                     /translation="MSVDEAAAQGDLDELIRHVDRLTDEQDWTELVRLRDKAKAAIER
                     GFQLWPAASYAEYRMALEAPGDWAAQMLVEGAGWMALGPLHEVAASTHQWADLTRNPG
                     GAHALLAAHERVLRGEDLRGQLDDLPQIFDVPAVLQPWEPDYALATYKPDKAEFPSPP
                     APVPLPTLFGEPGPRVDDHTVDAALTELAKTWADESNGRLETIAVEGDAPSAVAALGL
                     RSGRMVEITAGEAMAHMAWAAASGGAHGRRRGSATGRARAWWAMTTLTGLDETAELHP
                     DELGQAADELTWLWWDAAEPETGWRLRLAVADQTDNLAWAINAQDATT"
     gene            complement(1611..2306)
                     /locus_tag="VFP02_06645"
     CDS             complement(1611..2306)
                     /locus_tag="VFP02_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HET9542740.1"
                     /translation="MTDDLTLLREGEMMVEGRMPYSSNGTYLVRVALDGVEELAVYKP
                     VRGERPLWDFPSGLARREVAAYELSHALDWELVPPTVLRGGPGGEGSVQRFVDADFSE
                     HYFTLREDEAHDDTFRRLCAFDLVANSADRKGGHCLIDGDGHIWAIDNGLSFHQEFKV
                     RTVIWDYAGEPLPDEVCTGLSALVRDGLPESLAALLDPFERDAVLTRAGALLQTGSLP
                     HDHTGHRYPWPLV"
     gene            complement(2303..2800)
                     /locus_tag="VFP02_06650"
     CDS             complement(2303..2800)
                     /locus_tag="VFP02_06650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HET9542741.1"
                     /translation="MSDFDLREPDRFTAGAVGEPGSRVFFLQAVEGTQVVSLRLEKQQ
                     VGALGQYLQGVLSDLPVVTADELPTDLDLVEPVVAEWIVGTLAVAYDGAVDRVVLQAD
                     ELVDEEADEDESGHARFHLTRPQVVAFCAHAEELVAAGRPPCPICGQPLDPDGHICPR
                     SNGHK"
     gene            complement(2814..>3098)
                     /locus_tag="VFP02_06655"
     CDS             complement(2814..>3098)
                     /locus_tag="VFP02_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9542742.1"
                     /translation="GFRFPKGESFSEMQLRITDALAQLRARHPGQRVVAVSHADPIKA
                     AVAHAMGTHLDLFQRVVVSPCSVSAVALTDDGPLVLAVNAVDDLKRLVPS"
BASE COUNT          414 a         1059 c         1169 g          456 t
ORIGIN      
        1 cggggcctgg gcgaggccgg caagtacgag atgatcgagc agctggccaa gctcctcaag
       61 gcggctccgg gcgcgtcccg ggccatcgtg gacgccggct gggtcccgta ctcgtaccag
      121 gtgggccaga cgggcaaggt cgtcaagccg accgtctaca tcgcctgcgg catctccggt
      181 gccacccagc acctggtcgg gatgaagggc tccaagaaca tcatcgccat caacaaggac
      241 gccgaggcgc ccatcttctc ggtggccgac ctcgggatcg tcggggacgt ccacaaggtg
      301 ctccctcagg tgatcgaggc gctccagaac cggtagtcgc gcgctcgcgc cgtcctcggg
      361 gcttcgggct ccgggggcgg cgtcggtgcc tcccttgctc ctgacgcaaa cgagcgaaac
      421 cgtcgcggcg gaaggccccc acgccgcccc ctatgactca ggttcccacc gtctcgcgtg
      481 actgcccccg ggggagcgcc gcggtccttt cggtcgggag gggagggctg gagggcggcg
      541 tgggacttgc gggggacggg ctggccccct tggttgccat cggttcgtcc agcccgacgg
      601 gctcgcatcg cccgtgcgtc aggtggtggc gtcttgggcg ttgatggccc aggcgaggtt
      661 gtcggtttgg tcggcgacgg cgaggcgcag gcgccaaccg gtttcgggtt cggcggcgtc
      721 ccaccagagc caggtcagct cgtcggcggc ttggccgagc tcgtcggggt ggagctcggc
      781 ggtctcgtcc aggccggtga gggtggtcat cgcccaccag gcccgggctc ggcccgtagc
      841 gctgccccgt cgtcgtccgt gggcgccgcc gctggccgcg gcccacgcca tgtgggccat
      901 ggcctcgccg gccgtgatct cgaccatgcg accgctgcgg aggccgaggg cggcgacggc
      961 actgggtgcg tcgccttcga cggcgatggt ctcgagcctc ccgttcgact cgtcggccca
     1021 ggtcttggcc agctcggtca gggccgcgtc gacggtgtgg tcgtcgaccc gtggtccggg
     1081 ctcgccgaac agggtcggaa gggggacggg agcgggtgga ctcgggaact cggccttgtc
     1141 gggcttgtag gtggcgagcg cgtagtcggg ctcccacggc tgcagcaccg cggggacgtc
     1201 gaagatctgg gggaggtcgt cgagctgtcc gcggagatcc tcgcctcgca gcacccgctc
     1261 gtgggcggcg aggagggcat gggcgccgcc ggggtttcgg gtcaggtcgg cccactggtg
     1321 ggtactggcg gcgacctcgt ggagagggcc caaggccatc cagccggccc cttcgaccag
     1381 catctgcgcc gcccagtcgc cgggggcctc cagggccatg cggtactcgg cgtaggaggc
     1441 ggccggccac agctggaagc cgcgctcgat ggcggccttg gccttgtccc ggaggcggac
     1501 cagctcggtc cagtcctgct cgtcggtcag gcggtcgacg tgacggatca gctcgtcgag
     1561 gtcgccctgg gctgccgcct cgtcgacgct caacgagggg ggcgagggcc tcacaccagg
     1621 ggccaggggt agcggtggcc ggtgtggtcg tgagggaggg agccggtctg gagcagggcg
     1681 ccggcccggg tcaggacggc gtcgcgttcg aaggggtcga ggagcgcggc cagggactcg
     1741 ggcaggccgt cgcgcaccag ggcgctgagg ccggtgcaga cctcgtccgg cagcggctcg
     1801 ccggcgtagt cccagatcac ggtcctcacc ttgaactcct ggtggaagct gagcccgttg
     1861 tcgatggccc agatgtggcc gtcgccgtcg atgaggcagt ggcccccctt gcgatcggcg
     1921 ctgttggcca ccaggtcgaa ggcgcagagc cgccggaagg tgtcgtcgtg ggcctcgtcc
     1981 tcccgcaggg tgaagtagtg ctcgctgaaa tcggcgtcca cgaagcgctg gaccgagccc
     2041 tcgccccccg ggccgccgcg gaggaccgtg ggcggtacca gctcccagtc cagggcgtgg
     2101 gacagctcgt aggcggccac ctcgcgccga gccaagccgc tgggaaagtc ccagagcgga
     2161 cgctcgcccc gcaccggctt gtagacggcc agctcctcga ccccgtcgag ggccacccgc
     2221 accaggtagg tgccgttcga gctgtagggc atgcgcccct cgaccatcat ctcgccctcc
     2281 cggagcaggg tgaggtcgtc ggtcacttgt ggccgttgct ccgggggcag atgtggccgt
     2341 cgggatccag cggctggcca cagatcgggc acggcggccg gccggcggcc acgagctctt
     2401 cggcgtgcgc gcagaaggcc acgacctggg gccgggtcag gtggaagcgg gcgtggccgc
     2461 tctcatcctc gtcggcttcc tcgtcgacca gctcgtcggc ctggagcacc acccggtcca
     2521 ccgcgccgtc gtaggcgacg gcgagggtgc ccacgatcca ctcggccacg acgggctcga
     2581 ccaggtccaa gtcggtgggc agctcgtcgg cggtgaccac cgggaggtcg gacaggaccc
     2641 cctgcaggta ctggcccagc gcacccacct gctgcttctc caggcggagg ctgaccacct
     2701 gggtcccctc gacggcctgg aggaagaaga cccgagagcc gggctcgccg accgcaccgg
     2761 cggtgaaccg gtcgggctcc cgcaggtcga aatcgctcat cggcggttct ccctcacgac
     2821 ggcaccagcc gcttcaggtc gtcgaccgcg ttcaccgcca gcaccagggg accgtcgtcg
     2881 gtcagggcca cggcgctcac cgagcacggc gacacgacca ctcgctggaa caggtccagg
     2941 tgcgtgccca tggcgtgggc caccgcggcc ttgatcgggt cggcgtggga aacggccacc
     3001 acccgctggc cggggtggcg ggcccggagc tgggccaacg cgtcggtgat ccgcagctgc
     3061 atctcgctga aggactcgcc tttcgggaag cggaagcc
//
LOCUS       DASTFV010000360         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1322904, whole genome shotgun sequence.
ACCESSION   DASTFV010000360 DASTFV010000000
VERSION     DASTFV010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322904"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFP02_06660"
     CDS             <1..496
                     /locus_tag="VFP02_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET9542743.1"
                     /translation="SELMFFAGLFAAYFTLRSANPVWPSAAADLDEGRAAIFTTVLVV
                     SSLTMHRSVRAAEADRRDEAMRWLTATFLLGLVFVVNQALEYAHLGFGIDTDAYGSIF
                     WLLTGFHWLHVVAGLILMVALARLSWPGTRAPVGVHHQVVGYYWHFVDVVWVAVFTAV
                     YLIQ"
     gene            505..1251
                     /locus_tag="VFP02_06665"
     CDS             505..1251
                     /locus_tag="VFP02_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET9542744.1"
                     /translation="MLGVVTVVALSGLGGLHAAAQSSGDQPELADDPELVAAGQELFA
                     TSCSSCHGLAGEGSVNGPRIADAGEAAADFQLRTGRMPAADIEGQQPGKDPAYGDHEI
                     QALVAYVGSLGDGPRIPDVDPARGDLANGGELYRANCAACHNASGIGGALSYGSEAPS
                     VRDAGPVQVGEAMRTGPGQMPVFGPDSFSPQDLDDIARYIQELAEPSRRGGLSLGSVG
                     PVTEGFVAVLFALGSIVLATRWITARRRAS"
     gene            1248..2132
                     /locus_tag="VFP02_06670"
     CDS             1248..2132
                     /locus_tag="VFP02_06670"
                     /inference="COORDINATES: protein motif:HMM:NF039285.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET9542745.1"
                     /translation="MTDPSTEATDDEAPEAAPSATAPVIAFCASAAAAVGLAVVYSLG
                     GQPQAEGILLAIALGGIGFGLVSWSRRFMPDDVAVEDRGRVASDPEEIQALLDDLGAE
                     QAQVGRRHLLVRMLVVAGGALGLAALFPIRSLGPRPGRGLHTTPYAQGDLRLVSEEGA
                     PVRVDELAVNGVITVFPEGYEGQEDSQTLLLNLPEGELQPRPGREDWTVGGLVAFSKI
                     CTHAGCPVGLYEEQTGLLLCPCHQSTFDVSEAARPVFGPATRSLPQLPLGTDDEGYLI
                     ATGDFSGPVGPGFWDRDR"
     gene            2129..>2697
                     /locus_tag="VFP02_06675"
     CDS             2129..>2697
                     /locus_tag="VFP02_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HET9542746.1"
                     /translation="MILRRLLDWADSRTGVAGTTRRYVDKIFPNQWSFLLGEVTLYSF
                     IILVATGTWLAFFFQPSTAETIYDGSYAPLQGVAMSKAYASALDLSFDVRAGLVMRQM
                     HHWAALLFVAAMTAHLARVFFTGAFRRPRELNWIIGVVLLILAIVNGFAGYSMLDDQL
                     SGTGLRIAYSVVLSIPLAGTWMASLFFGG"
BASE COUNT          340 a         1028 c          900 g          429 t
ORIGIN      
        1 ctccgagctc atgttcttcg ccgggctgtt cgccgcctac ttcaccctcc gatcggccaa
       61 ccccgtctgg ccgtccgccg cggctgacct cgacgagggg cgggccgcca tcttcaccac
      121 cgtcctggtg gtcagcagcc tgaccatgca ccggtcggtc cgggcggccg aagccgaccg
      181 tcgggacgag gcgatgcgct ggctcaccgc caccttcctg ctcggcctcg ttttcgtcgt
      241 caaccaggcc ctcgagtacg cgcacctcgg cttcggcatc gacaccgatg cctacggatc
      301 gatcttctgg ttgctgaccg gctttcactg gctccacgtc gtcgccggcc tcatcctgat
      361 ggtggcgttg gcccggctca gctggcccgg cacccgggcc cccgtcgggg tccaccacca
      421 ggtcgtgggc tactactggc acttcgtcga cgtcgtctgg gtcgccgtct ttaccgcggt
      481 ctacctgatc cagtgagggg gtcggtgctc ggcgtggtga cggtggtggc gctgagcggg
      541 ttgggcggcc tgcacgccgc cgcccaatcg agcggcgacc agcccgaact ggccgacgac
      601 cccgagctgg tcgcggccgg ccaagagctc ttcgccacga gctgctccag ctgccacggg
      661 ctggccggcg agggcagcgt caacggtccc cgcatcgccg acgcagggga ggcggcggcc
      721 gacttccagc tcaggaccgg gcgcatgccc gccgccgaca tcgagggcca gcaaccgggg
      781 aaggaccccg cgtacggcga tcacgagatc caggcgctgg tcgcctatgt cggatccctg
      841 ggcgacggcc cccgcatccc cgacgtggat ccggcccgag gcgacctggc caacggcggc
      901 gagctctacc gggccaactg cgcggcctgt cacaacgcca gcggcatcgg tggggcgctg
      961 agctacggca gcgaggcccc aagcgtgcgc gacgcggggc cggtccaggt gggcgaggcg
     1021 atgcgcaccg gtcccgggca gatgcccgtg ttcgggcccg actcgttctc gccccaagac
     1081 ctggacgaca tcgcccggta catccaggag ctggctgagc cgtcgcgccg gggcggattg
     1141 agcctgggca gcgtcggtcc ggtgacggaa gggttcgtcg ccgtcctctt cgccctcggc
     1201 agcatcgtcc tcgccacccg atggatcacc gcccggagga gggcctcgtg acggacccct
     1261 ccaccgaggc gaccgacgac gaggcgcccg aggccgcccc ctcggccacc gccccggtca
     1321 ttgccttctg cgccagtgcg gccgccgccg tcggcctcgc cgtggtctac tccctcggcg
     1381 gccaacccca ggccgaaggc atcctgctgg ccatcgccct cggggggatc gggttcggcc
     1441 tcgtgtcctg gtcccgccgc ttcatgcccg acgacgtcgc cgtcgaggac cgcggcaggg
     1501 tggcgtccga tcccgaggag atccaggccc tcctcgacga cctcggcgcc gagcaggccc
     1561 aggtcgggcg ccgacacctg ctcgtgcgca tgctcgtcgt cgccggcggc gccctggggc
     1621 tggccgccct gttcccgatc cgctcgctcg gtccccggcc gggccgcggg ctgcacacca
     1681 cgccctacgc ccagggcgac ctgcgcctcg tatccgaaga gggcgcgccc gtccgggtcg
     1741 acgagctggc cgtgaacggt gtcatcaccg tgttccccga gggctacgag ggccaggagg
     1801 acagccagac gctgctgctc aacctccccg agggcgagct ccaaccccgg cccgggcggg
     1861 aggactggac ggtcggcggg ctggtggcct tctccaagat ctgcacccac gccggctgcc
     1921 cggtggggct gtacgaagag cagacgggcc tgctgctctg cccgtgccac cagtcgacct
     1981 tcgacgtcag cgaggccgcc cggcccgtgt tcggtcccgc cacgcgctcg ctcccccagc
     2041 tcccgctggg caccgacgac gagggctacc tgatcgcgac cggcgacttc tccggccccg
     2101 tcgggcccgg cttctgggac cgcgaccgtt gatcctgcgc cggctcctcg actgggccga
     2161 ctcccgcacc ggcgtcgccg gcaccacccg gcgctacgtc gacaagatct tcccgaacca
     2221 gtggtcgttc ctgctcggcg aggtgacgct ctactccttc atcatcctgg tcgccacggg
     2281 gacctggttg gcgttcttct tccaaccgag cacggccgag acgatctacg acggcagcta
     2341 cgcccccctc cagggggtgg ccatgagcaa ggcctatgcc tcggccctcg acctgagctt
     2401 cgacgttcgg gccgggctgg tcatgcgcca gatgcaccac tgggcggcgc tcctgttcgt
     2461 cgccgcgatg acggcccacc tggcccgggt gttcttcacg ggggccttcc gccgccctcg
     2521 ggagctcaac tggatcatcg gcgtggtcct gctcatcctg gccatcgtca acggcttcgc
     2581 cggctactcg atgctcgacg accagctgtc gggaacgggt ctgcgcatcg cctactccgt
     2641 cgtcctgtcg attccgctgg ccggcacctg gatggcgtcg ctcttcttcg gcggcga
//
LOCUS       DASTFV010000361         2521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1326039, whole genome shotgun sequence.
ACCESSION   DASTFV010000361 DASTFV010000000
VERSION     DASTFV010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326039"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFP02_06680"
     CDS             <1..485
                     /locus_tag="VFP02_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HET9542747.1"
                     /translation="DGHPTTLGACSGAVAGLVAITPCAGFVGGLSPLYIGLGVGVVCY
                     FAISMKYKFGYDDSLDVVGVHLVGGLFGTLALGLFADSSINGLVTNEGLFLGGGADLL
                     LDQAIAAAATIGFAFVATMIIGKVIDMTVGLRVSEADELTGLDLSQHAEAGYNLADHS
                     "
     gene            514..852
                     /locus_tag="VFP02_06685"
     CDS             514..852
                     /locus_tag="VFP02_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET9542748.1"
                     /translation="MKQITAVIKPFKLDDVKTAMKGAGVQGMTVTEVKGFGRQGGHTE
                     VYRGAEYQVDLVPKVRLDAVVDDGDVAAVVEALIAAANTGDIGDGKVWVSDVFDITRI
                     RTGEKGTDAV"
     gene            994..>2521
                     /locus_tag="VFP02_06690"
     CDS             994..>2521
                     /locus_tag="VFP02_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET9542749.1"
                     /translation="MTKYKLSRFKDRDTRKVFGTILGGKMLGILGALALMQAFIWLFG
                     NVAEAAGLQEAATVSDDLVNPINTMWTLVAAFLVFFMQAGFMALEAGFARSRESANIM
                     MECIVDTCLCGILYWAVGFAFQFGTGNGLIGHEFFFLSDAPATYGTTGIGFLAFWLFQ
                     FAFADTCSTITSGAMVGRTGFKGDLLYSAGVSAIIYPIFAHWVWGPGGWLGNMTPSFL
                     NDFTGGALFRDFAGSTVVHTVGGFIALAGAIVLGPRLGRKFAKDGGGPTAPHDLNVAA
                     IGAVILWFGWYGFNPGSTLSALDYEGIGRVAANTTLAAAAGGLMAMAFVYPRSKKWDV
                     GMTINGFLGGLVAVTAPCYWISPLGAVIIGCIAGIVVPLGIDFLEARRVDDPIGAVAV
                     HGFCGIWGTLAVGLFATGDFGIPGPLGADTSGGVVEGLFYGGGLDQLKAQFIGSLTCV
                     VVISLAAFALMYAVNATGTLRIDRDGELEGIDIVEHGLTAYHMEFGQGMGYTSPPGAG
                     D"
BASE COUNT          382 a          865 c          842 g          432 t
ORIGIN      
        1 aggacgggca cccgaccacc ctgggggcct gctccggtgc ggtcgccggc ctggtcgcca
       61 tcaccccctg cgccggcttc gtcggcgggc tgtccccgct ctacatcggc ctgggggtcg
      121 gcgtggtctg ctacttcgcc atctcgatga agtacaagtt cggctacgac gactccctcg
      181 acgtggtcgg cgtgcacctg gtcgggggcc tcttcggcac cctcgctctc gggctcttcg
      241 ccgactccag catcaacggc ctggtcacca acgagggcct gttcctcggc ggcggcgccg
      301 acctcttgct cgaccaggcc atcgccgcgg cggccaccat cggcttcgcc ttcgtggcca
      361 cgatgatcat cggcaaggtc atcgacatga ccgtcggcct ccgcgtcagc gaggccgacg
      421 agctgaccgg cctggatctg agccagcacg ccgaagccgg gtacaacctg gccgaccact
      481 cctaacggca acgacgacga aaggaactgt gacatgaagc agatcacggc agtcatcaag
      541 ccgttcaagc tggacgacgt gaagacggcg atgaaggggg cgggcgtgca aggcatgacc
      601 gtcaccgagg tcaaggggtt cgggcgccaa ggtggtcaca ccgaggtgta ccgcggggcc
      661 gagtaccagg tggacctggt gcccaaggtc cggctggacg cggtggtcga cgacggtgac
      721 gtcgccgcgg tcgtcgaggc cctgatcgcc gccgccaaca ccggcgacat cggcgacggc
      781 aaggtgtggg tgagcgacgt gttcgacatc acccgcatcc ggaccggtga gaaggggact
      841 gacgccgtct aggtcagcga cgaagcaccg aacgaccggg gaggggccgc gaaggccgct
      901 ccccggtcgt tcagattttc gacacaactt cgacccctag gtgtcaccgg ggccacacag
      961 acgggcaaca cctgcttcgt aggttgcgcc gccatgacca agtacaagct ttcccggttc
     1021 aaggaccggg acacgagaaa ggtgttcggc accatcctcg gtgggaagat gttgggcatc
     1081 ctcggggcgc tggccctgat gcaggccttc atctggctct tcgggaacgt cgcggaggcc
     1141 gccggcctcc aagaggcggc gacggtgtcg gacgacctcg tcaacccgat caacaccatg
     1201 tggacgctgg tggcggcctt cctggtgttc ttcatgcagg cgggcttcat ggccctggaa
     1261 gcgggcttcg cccgttcccg ggagagcgcc aacatcatga tggagtgcat cgtcgacacc
     1321 tgcctgtgcg ggatcctgta ctgggccgtc ggcttcgcct tccagttcgg caccggcaac
     1381 ggcctgatcg ggcacgagtt cttcttcctg tccgacgctc cggccaccta cggcacgacg
     1441 ggcatcggct tcctcgcgtt ctggctgttc cagttcgcct tcgccgacac ctgctcgacc
     1501 atcacctccg gggccatggt cggacggacc ggcttcaagg gggacctgct ctacagcgcc
     1561 ggtgtctcgg cgatcatcta cccgatcttc gcccactggg tctggggccc cggcggctgg
     1621 ctcggcaaca tgacccccag cttcctgaac gacttcaccg gcggcgcgct gttccgtgac
     1681 ttcgccggct ccaccgtggt ccacacggtc ggcgggttca tcgccctggc cggcgccatc
     1741 gtcctcggcc cccgcctcgg gcggaagttc gccaaggacg gcggtggccc gacggcgccc
     1801 cacgacctga acgtcgccgc catcggcgcg gtgatcctct ggttcggctg gtacggcttc
     1861 aaccccggct ccacgctctc ggccctggac tacgagggga tcggccgggt cgcggccaac
     1921 accacgttgg ccgcagccgc cggcgggttg atggccatgg cgttcgtcta cccgaggtcg
     1981 aagaagtggg acgtcgggat gaccatcaac ggcttcctgg gcggcctggt ggccgtcacg
     2041 gcgccctgct actggatctc accgctgggt gcggtgatca tcggttgcat cgccgggatc
     2101 gtggtcccgc tcgggatcga cttcctggag gcccgtcgcg tcgacgatcc catcggggcg
     2161 gtcgccgtcc acgggttctg cggcatctgg ggcaccctgg ccgtcgggct cttcgccacc
     2221 ggtgacttcg ggatccccgg accgctcggg gcggacacct ccggcggcgt cgtcgaagga
     2281 ctcttctacg gagggggcct ggaccagctc aaggcccagt tcatcggcag cctgacctgc
     2341 gtggtcgtca tctcgctggc cgcgttcgcc ctgatgtacg ccgtgaacgc cacggggacg
     2401 ctgcgcatcg accgggacgg cgagctcgag ggcatcgaca tcgtcgagca cgggctcacc
     2461 gcctaccaca tggagttcgg tcagggcatg ggctacacat cgcccccggg agcgggtgac
     2521 t
//
LOCUS       DASTFV010000362         3759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1327467, whole genome shotgun sequence.
ACCESSION   DASTFV010000362 DASTFV010000000
VERSION     DASTFV010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327467"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /gene="meaB"
                     /locus_tag="VFP02_06695"
     CDS             <1..1110
                     /gene="meaB"
                     /locus_tag="VFP02_06695"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET9542750.1"
                     /translation="RSSSSAAGSSLRAPTPSCRRPAWPRCSARGPTSPRPPCGCSSSS
                     TSNDTMTTTAEAIRSGDRRALAQGITLVESVRPDHRAEAVALVEELLPASGGAVRVGV
                     TGQPGAGKSTFIEALGLHLVDAGHRVAVLAVDPSSTRSGGSILGDKTRMGELGRRDEA
                     FIRPSPSGRTVGGVARRTREALLLCEAAGFDVVIVETVGSGQSDVAVAELVDVFVLLV
                     PPAGGDELQGIKRGIMELADVVVVTKADGDLVPAANRTAADHRHAVHLLHGAHPPPVV
                     SGSAFTGEGIDEAWAAVTEVHDRRSASGEQAERRSAQARTWLWDEVRESLLDELRTDA
                     TVADLAPELEAQVTAGTLSPAAAAGQLLERFRSGT"
     gene            complement(1134..1958)
                     /locus_tag="VFP02_06700"
     CDS             complement(1134..1958)
                     /locus_tag="VFP02_06700"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9542751.1"
                     /translation="MFLVLGQPRSGTTLVAQCLNGHPDLVLPDETDVVVPLAFLVDRI
                     EEPDLGRELATTLITHSRRFAASIGEYLDAERVAAVVGAAPWTLNGILTDLYAAVAEA
                     GGGRRGGDKSPNDLKFQRILLTARLYGPNLPVVHVVRDVRDVMASFRDLGWAQGVPEG
                     LARFWAANNLLVQTSVPRQESPYLLVRYEDLVADPDREFRRICELLGVEWDPGMIDDE
                     RRYQQFAGHRDIGQHAQTFQPISGARVGRYTDEFDAETLGHITDLAREGLEAFGYE"
     gene            complement(1966..>3759)
                     /locus_tag="VFP02_06705"
     CDS             complement(1966..>3759)
                     /locus_tag="VFP02_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9542752.1"
                     /translation="VLWLQRYAAARSVLHYLGIAPLAFAALFIFSSEAGSLVFTEDAQ
                     VVELEAGTGGPVAVLVLDELPLASLLDRNGHINEQRFPNFARLADQSTWYRNATAVNP
                     STPESVPSILTGQYPESGRLPTSADRPINIFTLLGGEYRVDAFEGVTDLCPDVVCIPD
                     EQHLSVSSFVDDVRGALGDAGVVFGHLTLPASLREDLPTLGQSWAGFLETPGDDDSTS
                     PSPEDLVAQEPVDDRTVLTDFLSGRAQQARDRGGQGRDLVPLIDGYSAATGTLFVAHD
                     PFLPHRPWHISPTGAAYQSQVGGVTAGEEEWPDSPAFVRRVLQRHLLQVGYADTLLGR
                     LLDRFEAAGTLDEATIAVVADHGMAFEVGGRARDPSDSNVQEIYRVPFFMKAPGQRAG
                     DGRISDQNALLVDVLPTIMDLIDVEPPDNAEFDGQSLVAAGFDRADDDKPVFYGNGPR
                     VVPGDFADVVPIALRNAGYVNDGGWVSLLQVGPAGHLVNQPVDAVERSRPVEGEWEID
                     QERSLRDTPDVRARSIAVAGRLALDDEDIDLPNQVLIAIDGILAGIGDLDRSDGEFAA
                     LIDERRLTVGHHDVALYLPTERGTIQRIQES"
BASE COUNT          529 a         1298 c         1406 g          526 t
ORIGIN      
        1 aggtcctcgt cgtctgcggc ggggtcatcc ctccgggcgc ccacgccaag ctgcaggagg
       61 ccggcgtggc cgcggtgttc ggcccgggga ccaacatccc caaggccgcc ctgcgggtgc
      121 tcgagctcct cgaccagtaa cgacaccatg acgacgacgg cggaggcgat ccgctcgggt
      181 gaccggcggg ccctggccca gggcatcacc ctcgtggagt ccgtgcgccc tgaccaccgg
      241 gcggaggccg tggccctcgt cgaggagctc ctccccgcct cgggcggtgc ggtccgggtc
      301 ggcgtcacgg gccagcccgg cgccgggaaa tcgaccttca tagaagccct cggcctgcat
      361 ctggtggacg cggggcaccg ggtcgccgtg ctggcggtcg acccctccag cacccgatcg
      421 gggggctcga tcctcggcga caagacccgc atgggcgagc tgggccgacg cgacgaggcc
      481 ttcatccgcc cgtcaccgtc ggggcgcacg gtgggcggcg tggcccgccg aacccgggag
      541 gccctcctcc tgtgtgaggc cgccggattc gacgtggtca tcgtggagac cgtgggctcc
      601 ggacagtccg acgtcgccgt ggccgagctg gtggatgtct tcgtcctgct cgtcccgccc
      661 gcgggtggcg acgagctgca gggcatcaag cgggggatca tggagctggc cgacgtggtc
      721 gtggtgacca aggccgatgg cgacctcgtg cctgcggcca accgcaccgc cgccgaccac
      781 cggcacgccg tgcacctgtt gcacggggct caccccccac cggtcgtgag cggctcggcc
      841 ttcaccggcg agggcatcga cgaggcctgg gcggccgtga ccgaggtcca cgaccgtcgg
      901 tcggcgtccg gcgaacaggc cgagcggcgc tcggcccagg cccgcacgtg gctctgggac
      961 gaggtgcgcg agtccctgct cgacgagctg cgcaccgacg ccaccgtcgc cgacctggct
     1021 cccgagctcg aagcccaggt caccgccggc accctctcgc ctgccgctgc cgccggccag
     1081 ctcttggagc gcttccgttc tggaacctga acgagttcct ggttccagaa cggttactcg
     1141 tagccgaacg cctcgagccc ttctcgggcg aggtcggtga tgtggccgag ggtctcggcg
     1201 tcgaactcgt cggtgtaacg gccgacgcgt gccccactga tgggctggaa cgtctgggcg
     1261 tgctggccga tgtcccggtg gccggcgaac tgctggtagc gccgctcgtc gtcgatcatg
     1321 cccgggtccc actcgacgcc caggagctca cagatccggc ggaactcccg gtcggggtcg
     1381 gcgaccaggt cctcgtagcg gacgagcaga tagggactct cctgccgggg cacgctcgtc
     1441 tggacgagga ggttgttggc cgcccagaag cgggccagcc cctcgggcac gccctgggcc
     1501 cagcccaggt cccggaacga ggccatgacg tcccggacat cgcgcaccac gtgcacgacc
     1561 ggcaggttgg ggccgtagag gcgggccgtc aggaggatgc gctggaactt gaggtcgttg
     1621 ggcgacttgt ccccaccgcg gcggccgccg ccggcctcgg ccacggcggc gtagaggtcg
     1681 gtcaggatcc cgttcagggt ccatggcgcg gccccgacga ccgccgccac acgctcggcg
     1741 tccaggtact ccccgatgct ggccgcgaag cgacggctgt gggtgatcag cgtcgtggcc
     1801 agctcccggc cgaggtccgg ttcctcgatg cggtcgacca ggaaggccag cgggacgacg
     1861 acatcggtct cgtcggggag caccaggtcg gggtggccgt tgaggcactg cgccaccagc
     1921 gtggtgcccg atcggggctg gccgagcacg aggaacacgg cggcctcagg actcctggat
     1981 ccgctggatg gtcccccgct cggtgggcag gtacagcgcg acgtcgtggt ggcccacggt
     2041 gaggcggcgc tcgtcgatga gcgccgcgaa ctccccgtcg ctgcggtcca ggtccccgat
     2101 gccggccagg atgccatcga tggcgatcag cacctggttg ggcaggtcga tgtcctcgtc
     2161 gtcgagagcc agccggccgg cgacggcgat cgatcgggcc cggacgtcgg gcgtgtcccg
     2221 cagcgagcgc tcctgatcga tctcccactc gccttcgacc ggtcgtgagc gctccacggc
     2281 gtccaccggc tggttcacga gatggccggc cgggcccacc tgcagcaggc tgacccaccc
     2341 cccgtcgttc acgtagccgg cgttgcgcag agcaatgggg accacgtcgg cgaagtcgcc
     2401 gggcaccacc cgggggccat tgccgtagaa gacgggcttg tcgtcgtcgg cccgatcgaa
     2461 accggccgcg acgagggact ggccgtcgaa ctcggcgttg tcggggggct cgacgtcgat
     2521 gaggtccatg atcgtgggca ggacgtcgac cagcagcgcg ttctggtcgc tgatgcgccc
     2581 gtcaccggcc cgctggcccg gggccttcat gaagaacggc acccggtaga tctcctggac
     2641 gttgctgtcc gacgggtccc gggcccggcc gccgacctcg aaggccatgc cgtggtcggc
     2701 caccaccgcg atggtggcct cgtcgagggt cccggccgcc tcgaaccggt ccagcagccg
     2761 gccgagcagg gtgtcggcgt agccgacctg gagcaggtgg cgctggagga cccggcgcac
     2821 gaaggccggc gagtccggcc actcctcctc gcccgccgtc accccgccca cctgggattg
     2881 gtaggccgcg ccggtcgggc tgatgtgcca cggccggtgg ggcaggaagg ggtcgtgggc
     2941 gacgaacagg gtgccggtgg cggcgctgta gccgtcgatc aggggcacga ggtcccggcc
     3001 ctgcccgcct cggtcccggg cctgctgcgc ccggccggag aggaagtcgg tcagcacggt
     3061 gcggtcgtcc accggctcct gcgcgaccag gtcctccggg gagggggagg tggagtcgtc
     3121 gtcacccggc gtctccagga agccggccca cgactggccg agggtcggca ggtcctcgcg
     3181 gagcgacgcc ggcagggtga ggtggccgaa gaccactccc gcgtcgccga gcgcgccgcg
     3241 aacgtcgtcg acgaacgagc tgacgctcag gtgctgctcg tcggggatgc agacgacgtc
     3301 cgggcagagg tcggtgacgc cttcgaaggc atccacccgg tactcgccac ccaagagggt
     3361 gaagatgttg atgggccggt cagccgaggt ggggagccgg cccgactcgg ggtactgccc
     3421 ggtgaggatc gacggcaccg attccggggt ggacgggttg accgcggtcg cattgcggta
     3481 ccacgtcgac tggtcggcca ggcgggcgaa gttggggaag cgctgctcgt tgatgtgccc
     3541 gttccggtcg agcagggagg ccaacgggag ctcgtcgagg acgagcacgg ccacggggcc
     3601 gcccgtgccc gcctccaact cgacgacctg ggcatcctcg gtgaagacca gggagccggc
     3661 ctccgaggag aagatgaaca gggccgcgaa ggccaggggg gcgatgccga ggtagtggag
     3721 gacggatcgg gcggcggcgt agcgctgaag ccacaacac
//
LOCUS       DASTFV010000363         3409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1332678, whole genome shotgun sequence.
ACCESSION   DASTFV010000363 DASTFV010000000
VERSION     DASTFV010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332678"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2764)
                     /gene="rpoB"
                     /locus_tag="VFP02_06710"
     CDS             complement(<1..2764)
                     /gene="rpoB"
                     /locus_tag="VFP02_06710"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET9542753.1"
                     /translation="MSDPRTSTSLRLSFGKIPEILPIPDLIAIQRQSFEWLITDGLRE
                     TFDEISPIEDFTGQMALTFGEHRFEEPEHSVEECRERDNTYSRKLHVQAEFQNRTTGE
                     IKSQSVFMGDFPVMTDQGTFIINGTERVVTSQLVRSPGVIFEQEPDKTLDKLLTSCRV
                     IPARGAWLEFDVDKKDTVGVRLDRKRRLPVTVLLRGLGMGDEQIRERFGESETMMGTL
                     EKDHTKTEEDALVEIYRRLRPGEPPSAESGRQLLHGLFFQSKRYDLAKVGRYKINKKL
                     GLDPEQHKALTLTTDDVVATVDYLVRLHEGDPSTSTDDQDHFGNRRLRSVGELIQNQV
                     RIGLSRMERVVRERMTTQDAEAITPQTLINIRPVVAAIKEFFGTSQLSQFMDQTNPLA
                     ALTHRRRLSALGPGGLSRERAGFEVRDVHPSHYGRMCPIETPEGPNIGLIGNLSTYAR
                     INEYGFVETPYRKVVNGRVTDQVDYLTADDEDLHVIAQANAAVDARGNFVTDRVLVRQ
                     GSRSAGVTGQRSVSRADFGAGQGAGVTGEVANVPPDEVDYMDVSPRQMVSVATALIPF
                     LEHDDANRALMGANMQRQAVPLLRSETPLVGTGMERRVAVDAGGVVVADAPGTVEDVT
                     ADQVTVRHADGSARVYRMRKFERSNQGTCINQKPIVLEGAKIAAGDVLADGPCTDTGE
                     LALGKNLLVAFMPWEGHNFEDAIILSERLVKDDVLTSIHIEEHEVDARDTKLGAEEIT
                     RDIPNVSEDVLKDLDERGIIRIGAEVDPGDVLVGKVTPKGETELTPEERLLRAIFGEK
                     AREVRDTSLKVPHGETGKVIGVRVFSREAGDDLNPGVNQLVRVYVAQKRKISDGDKLA
                     GRHGNKGVISKILPEEDMPFLADGTPIDVILNPLGVPSRMNIGQVLETHLGWVAAHGW
                     SFDTLPD"
     gene            complement(3039..3389)
                     /gene="rplL"
                     /locus_tag="VFP02_06715"
     CDS             complement(3039..3389)
                     /gene="rplL"
                     /locus_tag="VFP02_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET9542754.1"
                     /translation="MTLLELSEFVKEFEETFNVSAAAPVAIAAGPGGAGAGAAAAEEA
                     EEQTEFDVVLNGAGEKKIQVIKEVRALTSLGLKEAKDLVDSAPKAVLERVPKDQADKA
                     KAQLEGAGASVELK"
BASE COUNT          463 a         1100 c         1254 g          592 t
ORIGIN      
        1 agtcgggcag ggtgtcgaac gaccagccgt gggcggccac ccagcccagg tgggtctcca
       61 gcacctggcc gatgttcatc cggctcggca cgccgagggg gttgaggatg acgtcgatcg
      121 gggtgccgtc ggccaggaag ggcatgtcct cctcggggag gatcttggag atgacaccct
      181 tgttgccgtg gcggccggcc agcttgtcgc cgtcggagat cttccgcttc tgggcgacgt
      241 agacccggac cagctggttc acgccggggt tgaggtcgtc gccggcctcg cgggagaaca
      301 cccgcacgcc gatgaccttg ccggtctcgc cgtggggcac cttcagggag gtgtcgcgga
      361 cctcgcgggc cttctccccg aagatggcgc gcagcaggcg ctcctcgggg gtcagctcgg
      421 tctcgccctt gggcgtgacc ttgccgacca ggacgtcgcc ggggtcgacc tcggccccga
      481 tgcggatgat gccccgctcg tcgaggtcct tgagcacgtc ctcggagacg ttggggatgt
      541 cgcgggtgat ctcctcggcc ccgagcttgg tgtcgcgggc gtcgacctcg tgctcctcga
      601 tgtggatcga ggtgaggacg tcgtccttga ccaggcgctc ggagaggatg atggcgtcct
      661 cgaagttgtg gccctcccag ggcatgaacg cgaccaggag gttcttgccg agcgccagct
      721 cgccggtgtc ggtgcagggc ccgtcggcca gcacgtcgcc ggcggcgatc ttggcgccct
      781 cgagcacgat cggcttctgg ttgatgcagg tgccctggtt ggagcgctcg aacttgcgca
      841 tccggtagac ccgggccgag ccgtcggcgt ggcggacggt gacctggtcg gcggtcacgt
      901 cctcgaccgt gcccggggcg tcggcgacga ccacgccgcc ggcgtcgacc gccacccgcc
      961 gctccatgcc ggtgccgacc agcggcgtct cggagcggag caggggcacg gcctggcgct
     1021 gcatgttggc gcccatcagg gcccggttgg cgtcgtcgtg ctccaggaac gggatcaggg
     1081 cggtggccac cgacaccatc tgccgggggc tgacgtccat gtagtcgacc tcgtcgggcg
     1141 gcacgttggc cacctcgccg gtgacgccgg cgccctgacc ggcgccgaag tcggcccggc
     1201 tgaccgagcg ctggccggtg acgccggccg agcggctgcc ctggcggacc aggacgcggt
     1261 cggtgacgaa gttgccccgg gcgtcgacgg ccgcgttggc ctgcgcgatc acgtgcaggt
     1321 cctcgtcgtc ggcggtcagg tagtcgacct ggtcggtgac ccggccgttg accaccttgc
     1381 ggtagggcgt ctccacgaag ccgtactcgt tgatccgggc gtaggtcgag aggttcccga
     1441 tgaggccgat gttcgggccc tcgggggtct cgatcgggca catgcggccg tagtgggacg
     1501 ggtggacgtc gcggacctcg aacccggccc gctcgcgcga cagcccgccc gggccgagcg
     1561 ccgacaggcg ccgccggtgc gtcaacgccg ccagcgggtt ggtctggtcc atgaactggc
     1621 tgagctggga ggtcccgaag aactccttga tcgcggccac caccggacgg atgttgatca
     1681 gcgtctgggg ggtgatcgcc tcggcgtcct gggtggtcat gcgctcgcgc acgacccgct
     1741 ccatccgcga cagaccgatg cggacctggt tctggatcag ctcgccgacc gagcgcagcc
     1801 gccggttgcc gaagtggtcc tggtcgtcgg tgctggtcga ggggtcgccc tcgtgcagcc
     1861 ggaccaggta gtcgacggtg gccaccacgt cgtcggtggt gagggtcagg gccttgtgct
     1921 gctcggggtc gaggcccagc ttcttgttga tcttgtagcg gccgaccttg gccaggtcgt
     1981 agcgcttgga ctggaagaac agcccgtgca ggagctggcg gccggactcg gcgctgggcg
     2041 gctcgcccgg gcggaggcgc cggtagatct cgaccagcgc gtcctcttcg gtcttggtgt
     2101 ggtccttctc cagcgtgccc atcatggtct cggactcgcc gaagcgctcg cggatctgct
     2161 cgtcgcccat gccgaggccg cgcagcagca cggtcacggg caggcggcgc ttgcggtcga
     2221 ggcggacgcc gacggtgtcc ttcttgtcga cgtcgaactc gagccaggct cccctggccg
     2281 ggatgacccg gcaggaggtg agcagcttgt cgagggtctt gtcgggctcc tgctcgaaga
     2341 tgacgccggg cgagcggacc agctgcgagg tcacgacgcg ctcggtgccg ttgatgatga
     2401 aggtgccctg gtcggtcatg accgggaagt cgcccatgaa gaccgactgg gacttgatct
     2461 caccggtggt gcggttctgg aactcggcct ggacgtggag cttgcgagaa taggtgttgt
     2521 ctcgctcccg acactcctcg acggaatgct cgggctcctc gaagcggtgc tccccgaagg
     2581 tgagcgccat ctggccggtg aagtcttcga tggggctgat ctcgtcgaag gtctcccgga
     2641 gaccgtcggt gatcagccac tcgaaggact ggcgctggat ggcgatgagg tcggggatgg
     2701 ggaggatctc ggggatcttg ccgaaggaga gtcgtaggga ggtcgaggta cgcgggtcgc
     2761 tcaacaagcg tccctcccct ttcgcgggaa gcaggtgtgg aagcgggtgg tgcggcggct
     2821 gggcacgcca aagcccgggg taggctggtc gaaggcgcgc gaagacatgc gaaggagcag
     2881 tgtagcgctt tcctacaccg ccgtcaacgt cgaactgagg gtctgcgtgg gcacctcact
     2941 tcgtcttgtc cgaccgagtt tgccgcccac tccccaaggt gtcaagggac aactacggag
     3001 agtgggcgac aggaacgcgc caggtgatga gcccccggct acttcagctc aacgcttgcg
     3061 ccggcgccct ccagctgggc cttggccttg tcggcctggt ccttggggac ccgctcgagc
     3121 accgccttgg gcgcggagtc gaccaggtcc ttggcctcct tgagccccag gctggtgagg
     3181 gcgcggacct ccttgatgac ctggatcttc ttctcgccgg ccccgttcag gacgacgtcg
     3241 aactcggtct gctcctcggc ctcctcggcc gcggcggcgc cggcgccggc cccgccaggc
     3301 ccggcggcga tggccacggg ggcggcggcg gacacgttga acgtctcctc gaactccttc
     3361 acgaactccg acagctccag gagggtcatc tccttgaagg cgtccagga
//
LOCUS       DASTFV010000364         3431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1345575, whole genome shotgun sequence.
ACCESSION   DASTFV010000364 DASTFV010000000
VERSION     DASTFV010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345575"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1122
                     /locus_tag="VFP02_06720"
     CDS             175..1122
                     /locus_tag="VFP02_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HET9542755.1"
                     /translation="MAPAEKVDKLERLMNLVAVLLDTMRPLSADELRELVGGYPEGGP
                     TFHRAFERDKEDLREMGVPLVVQPLPGVDPPRDGYRILRDEYYLPDPGLEADELAALH
                     LAVSTVQVGPTDDEALWKLGGVIGADGLGASPGGGGPVASLPTDARLPLLFGAVSERR
                     RVRLTHGDVERLVDPYRLDFRRGRWYVTGLDHEREDIRSYRLDRIEGDVTTEGPGKAF
                     ERPDDVRSGAERQPWELGDDEPVEARVLVDADQAGYAVHLTGEAAVAERHDDGSVELA
                     LPVSNPTAFRSFVLTFLEHAEVLSPDELRDDLVTWLEHL"
     gene            1122..2069
                     /locus_tag="VFP02_06725"
     CDS             1122..2069
                     /locus_tag="VFP02_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HET9542756.1"
                     /translation="MGRPSAGERLKRLLALLPWLAAHPGSTIAEISERFGLSPQQLED
                     DLAVVWMVGVPPYTPDQLIDVEFDGDRVSVNLGSYFQRPLRLTNQQALALVAAGQSLV
                     SVPGTDPEGPLARGLEKLATALQVDPAEAMAVHLGDAGSETLARLRAASEAGRRVRID
                     YYSYGRDERSRRDVDPHRIWAEGGGWYLAGWCHKAEGPRVFRLDRIAEVEVLDDAVST
                     MPEAGETATFSPTATDASVTLDLDPESSWVADYYPHDAVEEQEDGRILVELPVASTRW
                     LEQLLVRLGPSATVVALSEDLPAHPGADAARRVLARYRR"
     gene            2081..2722
                     /gene="lepB"
                     /locus_tag="VFP02_06730"
     CDS             2081..2722
                     /gene="lepB"
                     /locus_tag="VFP02_06730"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET9542757.1"
                     /translation="MTSPHTDAQADAPETDPEDESSRSSRSTTRSVIEWIAVIGGALI
                     VALIIKTFLIQAFYIPSGSMETTLDIGDRVLVNKLSYRVHDVNRGDLVVFEANEGAGD
                     CGQPVSDAAASASESGIKDLIKRVIGLPGDTVEGRDGQLLVNGRLLVEPYLDDGVTTG
                     DFAAVEIPEGCIWVMGDNRGDSRDSRSFGPIDEDQIVGRAFVRVWPLNDLSLL"
     gene            complement(2751..>3431)
                     /locus_tag="VFP02_06735"
     CDS             complement(2751..>3431)
                     /locus_tag="VFP02_06735"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET9542758.1"
                     /translation="FAHRGASAAAPENTLEAFRLAVELGASGLDSDVWVSNDGEAVLD
                     HDGLVGGWLRRRPMGTVLRHRLPGHVPTLGELYEACGTALPLSLDVKDPAALGAVLAA
                     ADAVGARGNLWLCHPDIDRLAGWREQAEGAHLVASTKLRAFADGTERAAAELSRLSID
                     AVNLHHTEWSGGLTTLFHRFELACLGWDAQLPRHLAELLDMGIDGVFSDHVDRMMEAI
                     QVEMGASS"
BASE COUNT          495 a         1272 c         1196 g          468 t
ORIGIN      
        1 acccagacca tggagtacct cgactacggc gagcccgtcg aggtcgacat ccccgcggac
       61 gacgagaccg tcgacatctc ggatctcctg gacagctgag ccggcgatcg ctccgggcgg
      121 gggcgcccca gcggtcgatc cgccgcgaag ccgccgacgt aggctcggcg gaccatggcg
      181 cccgcggaga aggtggacaa gctcgagcgg ctgatgaacc tcgtcgccgt cctgctcgac
      241 accatgcggc cgctgagcgc cgacgagctc cgggagctgg tcgggggcta cccggagggc
      301 gggccgacgt tccaccgggc tttcgagcgg gacaaggaag acctgcggga gatgggggtg
      361 ccgctcgtcg tccagcccct ccccggcgtg gaccctcccc gggacggcta ccgcatcctg
      421 cgggacgagt actacctgcc cgaccccggg ctggaggccg acgagctggc cgcgctccac
      481 ctcgcggtgt cgaccgtcca ggtcggcccg accgacgacg aggccctgtg gaagctgggc
      541 ggcgtgatcg gagccgacgg cctgggggcc agccccgggg ggggcgggcc ggtggcgtcc
      601 ctgccgaccg acgcccgcct gccgctgttg ttcggcgccg tctccgagcg tcgtcgggtc
      661 cggctgaccc acggcgacgt cgagcgattg gtcgacccgt accgcctcga cttccggcga
      721 gggcgctggt acgtcaccgg tctcgaccac gagcgggagg acatccgcag ctaccgcctc
      781 gaccgcatcg agggggacgt gacgacggag ggcccgggga aggccttcga gcggcccgac
      841 gacgtacggt ccggcgccga gcgccagccc tgggagctgg gcgacgacga gcccgtggag
      901 gcccgcgtgc tggtcgacgc cgaccaggcc gggtacgccg tccacctgac gggggaggcc
      961 gcggtcgccg agcgtcacga cgacggctcg gtcgagctgg ccctcccggt gagcaatccg
     1021 acggccttcc ggtccttcgt cctcaccttc ctcgagcacg ccgaggtcct cagccccgac
     1081 gagctgcgcg acgacctcgt gacctggctg gagcacctct gatgggccga ccctccgcag
     1141 gagagcgcct gaagcgcctt ctcgccctcc tcccgtggct ggccgctcat ccgggcagca
     1201 cgatcgccga gatcagcgaa cggttcggcc tgagccctca gcagctggag gacgacctgg
     1261 ccgtcgtctg gatggtcggc gtccccccgt acaccccgga ccagctcatc gacgtcgagt
     1321 tcgacggcga ccgggtgtcg gtcaacctgg gcagctactt ccagcgcccc ctgcgcctga
     1381 ccaaccagca ggccctggcg ctggtagccg ccggccagag cctcgtgtcc gtaccgggca
     1441 ccgacccgga gggtccgctg gcccgcggcc tcgagaagct ggccaccgcc ctgcaggtcg
     1501 acccggccga ggcgatggcc gtgcacctgg gcgacgcggg gagcgagacc ctggcccgac
     1561 tccgggccgc cagcgaggcc gggcgccggg tccgcatcga ctactactcg tacgggcgcg
     1621 acgagcgcag ccgccgcgat gtcgaccccc accgcatctg ggcggagggc gggggctggt
     1681 acctggcggg ctggtgccac aaggccgaag ggcctcgggt cttccgcctc gatcgcatcg
     1741 ccgaggtcga ggtgctggat gacgccgtgt cgaccatgcc cgaggcgggg gagaccgcca
     1801 cgttcagccc cacggcaacc gatgcctccg tgacactgga cctcgacccc gagtcttcat
     1861 gggtcgcgga ctactacccc cacgacgccg tcgaggagca ggaggacggg cggatcctgg
     1921 tcgagctgcc cgtggccagc actcgctggc tcgaacaact cctcgtccgg ctgggcccgt
     1981 cggccacggt cgtggctctc agtgaggacc tgcccgcgca tcccggagcc gacgccgccc
     2041 gccgagtcct ggcccggtac cggcggtagc ctcgcccgcg gtgacctccc cccacacgga
     2101 cgctcaggct gacgcgcccg agaccgatcc cgaagacgaa tcgagtcgct cgtcccgctc
     2161 caccacccgc tcggtcatcg agtggatcgc cgtcatcggc ggggccctga tcgtcgccct
     2221 gatcatcaag accttcctga tccaggcctt ctacatcccg tccggctcga tggagaccac
     2281 cctcgacatc ggcgatcggg tcctggtcaa caagctcagc taccgggtcc acgacgtgaa
     2341 tcggggcgac ctggtggtgt tcgaggccaa cgagggcgcc ggcgactgtg gccagccggt
     2401 ctccgacgcc gcggccagcg cgtccgagag cggcatcaag gacctgatca agcgggtcat
     2461 cggcctgccc ggcgacaccg tcgagggccg ggacggccag ctcctggtca acggtcggct
     2521 cctggtcgag ccctacctgg acgacggcgt caccaccgga gacttcgcgg ccgtcgagat
     2581 ccccgagggc tgcatctggg tcatgggcga caaccggggg gactcccggg acagccgctc
     2641 cttcggcccc atcgacgagg accagatcgt cggccgcgcc ttcgtacggg tctggcccct
     2701 gaacgacctg agcctgctgt gacatccgcg tggcttcgcg gcgcaaccgt ctagctgctg
     2761 gcgcccattt ccacctgtat cgcttccatc atgcggtcga catggtcgct gaagaccccg
     2821 tcgatcccca tgtcgagcag ctcggccagg tgacggggga gttgggcgtc ccaaccgagg
     2881 caggccagct cgaaccggtg gaacagcgtc gtcaagcccc cggaccactc ggtgtggtgc
     2941 aggttgacgg cgtcgatcga caggcgggac agctctgccg cggcccgctc cgttccgtcg
     3001 gcgaaggccc ggagcttggt cgacgccacg aggtgggccc cctcggcctg ctcccgccac
     3061 ccggccagcc ggtcgatgtc gggatggcag agccacaggt tcccccgggc gccgacggcg
     3121 tcggccgcgg ccagcacggc gccgagcgcc gccgggtcct tcacgtccag gctgagcggt
     3181 agcgccgtcc cgcaggcctc gtagagctcg ccgagggtcg ggacgtggcc ggggagccgg
     3241 tggcgcagca ccgtccccat gggtcgccgc cgcaaccagc ccccgaccag gccgtcgtgg
     3301 tcgagcacgg cctccccgtc gttgctgacc cagacgtcgg agtccagccc ggatgccccc
     3361 agttccaccg ccaagcgaaa ggcctcgagg gtgttctcgg gagccgccgc gctcgcaccc
     3421 cggtgggcga a
//
LOCUS       DASTFV010000365         6488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1346388, whole genome shotgun sequence.
ACCESSION   DASTFV010000365 DASTFV010000000
VERSION     DASTFV010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346388"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="VFP02_06740"
     CDS             <1..1257
                     /locus_tag="VFP02_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9542759.1"
                     /translation="DLDFLDGTKGAHVNISGVSGVATKTSYATFLLYSLFHSGVLGTE
                     AVNTKALIFNVKGEDLLFLDHDNTQLAEQERAKYAHLDLPVGAFRSMAVWAPPRRDDP
                     SATPDVAGRHGGVTSFFWTLEQFCQDELLPFLFADAEDDRQQYTMVVHNVTARLAADA
                     QAAGEGAVRIDGDTARTFRELVEIISNRVQDEGEADRWAGRAIGAGTVNAFIRRLHGS
                     IRHLDRLIRADQPDGDAHLVNDEAQVTVVDLHNLNDRAKRFVVGVVLRRAFDKKERQG
                     TARPLQFVVLDELNKYAPREGASPIKEILLDVAERGRSLGIILIGAQQTASEVERRIV
                     ANCAIRVVGRLDPAEAGRGEYGFLPPVQRDRATIIKPGTMLVAQPHLPLPLVTQFPFP
                     AWATRSAEAGVNPHATNQPDDPFEGI"
     gene            1265..2434
                     /locus_tag="VFP02_06745"
     CDS             1265..2434
                     /locus_tag="VFP02_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HET9542760.1"
                     /translation="MKLLHTSDWHVGKAMRGQSRADEHEAVLTEITAVAGAEAVDLVL
                     VTGDLFETSAPAPESEAIVYRHLLGLAATGAKVVVIAGNHDNPRRLAAVTPLLALAQV
                     TLLAEPAKADAGGVVHFETADGEKVNLALLPFVSQRGIVKADDLMAGQAVDHSATYAE
                     RLRRLVEHLTDGLDGEDAVNLLAAHAMVSGGVMGGGERQAHTIFQYELPGAAFPGSLH
                     YAALGHLHRTQAVPGAACPAWYAGSPLALDFGEETDRKAVLVVEAEPGAPATVRPVPL
                     ESGRALRTLRGSFTDLRELASDVGDAWLRVEVDEPARAGLAEEVRELLPHAVQVSARP
                     GEAAQGGARAARLGRSPDELFAEYLAEQDVEDERLLALFRELHDELSDSGSADAA"
     gene            2424..4823
                     /locus_tag="VFP02_06750"
     CDS             2424..4823
                     /locus_tag="VFP02_06750"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HET9542761.1"
                     /translation="MRPERLEVEGFTAFRETVAVDFDGADLFAFTGPTGSGKSSLIDA
                     MTFALYGSIPRLDKKAVAPVISQGLLQARVRLDFTVGDVGYTAVRIVRATSAGGATTK
                     EARLERRDADGGVETIAGDAKALTTQIEDLLGLSFEHFCTCVVLPQGEFAALLHAEPR
                     KRQDMLLALLDLGLYEAMGQAARSRATAAAAQVEVLDRQVAELADASEETLKEAERRV
                     GVLDRLVERIDEARPRLEELVTVIAAAKAEAEEAERRVATLTDLAVPDDVARLAAKAD
                     AARKELDQARKDHEAAAAFLEQAEAATGDLPDRVALRERLTLLARRAEQAERIRTGAT
                     LEGERTEVLAATVAVREEADADYAEAQVAVVTAKDRDRAAAIARDLVAGAPCPVCRQV
                     VHDVPSHSDAPDLTAAEKALADTTKAMRTAHDAERDAQRELDKVTALLTEVRQTVTDL
                     DEQLGEGLDQVAIDAALVDLDRREADLRTARETEKQRRRDLEGAERRAVTLADDEKAL
                     WVRFDEARDALVALGPPAAAREDLGAAWGVLVAWADEARPAHEVQAAEARARVEQSET
                     ERTAIEDRIRETCAELDVTVGDRTPRDAATEARERARTRAEGLAKDRQRAVELEAQRR
                     GLVERRQVAEELGRHLRADRFEKWILDEALQRLVEGAADLLRELSSGAYSLTLDAGGA
                     NFAVVDHANADAVRGAKTLSGGETFLASLALALALADQVADLAAGGSARLESIFCDEG
                     FGSLDLDTLDIVATALEELGASGRMVGVVSHVRELAERLPVRFEVRKGPTTSTVTRVD
                     A"
     gene            4894..5820
                     /locus_tag="VFP02_06755"
     CDS             4894..5820
                     /locus_tag="VFP02_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542762.1"
                     /translation="MRLAVEPWAPEYGSPTEEGGFTPQDPPVLDLELAPQDWEPLSPG
                     GAPVGTVYFVDGVQRIEASVVVTLDSGEARRGRCVSYGAGVVRCDGRATVVEAQARHE
                     FLAPDRAAQDIPTRHGTFTHYGVTSDDSGVLAKALHDRMADLEAEVARSAAPGADDLL
                     ITDGPLSGRRHGVKHAVGYVKTHPVAYLPEDLSQRVVTALLPGQRTPVFRLGAGRPAW
                     YVRLPTPIDHGWSGVVRCEAEDEVSGPELFALADRVTATLPRFASEGHKDPRAPQNLY
                     PIGGLEKELKRRLGDPRLWFRALRETALRSDA"
     gene            complement(5828..>6488)
                     /locus_tag="VFP02_06760"
     CDS             complement(5828..>6488)
                     /locus_tag="VFP02_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542763.1"
                     /translation="QASLFGVVALILAFGLALAVGRYDARRAAVVDDANTIGTTYLRA
                     QTLPEPERTESLELLRRYADTSIRLANTIPASAAFDAAAADGDELQRDLWALAGEALD
                     QSPEGSAVRLYVESLNRMIDQETVRTSVLNNRVPDAVLALEALAAACAVALLAFLLAL
                     LGRGIGPALIAATLVALLLLVTFDLDRPTRGTIKVPDKPLTDLRTSMDEPPAAAAPTN
                     P"
BASE COUNT          923 a         2399 c         2335 g          831 t
ORIGIN      
        1 gacctcgact tcctggacgg gaccaagggc gcccacgtca acatcagcgg cgtatcgggc
       61 gtggccacca agaccagcta cgccaccttc ctgctctaca gcctcttcca ctccggtgtc
      121 ctggggacag aggcggtcaa caccaaagcc ctgatcttca acgtgaaggg cgaagacctc
      181 ctcttcctcg accacgacaa cacccagctg gccgaacagg agcgagccaa gtacgcccac
      241 ctcgacctgc ccgtcggggc cttccgcagc atggccgtct gggccccgcc ccggcgagac
      301 gacccgtccg ccacgcccga tgtcgccggc cgccacggtg gggtcacgtc cttcttctgg
      361 acgctggagc agttctgcca ggacgagctg ttgcccttcc tgttcgccga cgccgaggac
      421 gaccgccagc agtacacgat ggtcgtgcac aacgtgaccg cccggctggc cgccgacgcc
      481 caggccgcgg gggagggggc ggtgcgcatc gacggcgaca cggcccgcac gttccgcgag
      541 ctggtcgaga tcatctcgaa ccgggtccaa gacgagggcg aggccgaccg ctgggccggt
      601 cgggccatcg gggcgggcac cgtcaacgcc ttcatccgcc gcctgcacgg ctccatccgc
      661 cacctggacc ggctgatccg ggccgatcag cccgatggcg atgcccacct cgtgaacgac
      721 gaggcccagg tcaccgtcgt cgacctccac aacctgaacg atcgggccaa gcgcttcgtc
      781 gtcggcgtcg tcctgcgccg ggccttcgac aagaaggagc ggcaaggcac ggcccgcccg
      841 ctgcagttcg tggtcctgga cgagctcaac aagtacgcgc cacgcgaggg ggccagcccc
      901 atcaaggaga tcctcctgga cgtggccgaa cggggccgct cgctcgggat catcctcatc
      961 ggcgcccagc agacggccag cgaggtcgag cgccggatcg tggccaactg cgccatccgg
     1021 gtcgtcggtc gcctcgaccc ggccgaggcc gggcggggcg agtacggctt cttgcccccg
     1081 gtacagcggg accgggccac gatcatcaag ccgggcacga tgctggtggc ccagccccac
     1141 ctgccgctgc cactggtgac ccagttcccg ttccccgcct gggccacgcg ctcggccgag
     1201 gccggcgtca acccgcacgc cacgaaccag cccgacgacc ccttcgaggg gatctgaggt
     1261 cccggtgaag ctgctccaca ccagcgactg gcacgtcggc aaggccatgc ggggccagag
     1321 ccgggccgac gagcacgagg cggtgctgac cgagatcacc gcggtggcgg gcgccgaagc
     1381 ggtcgacctg gtcctggtca cgggagacct gttcgagacc tcggcccccg ccccggagtc
     1441 ggaggccatc gtctaccgcc acctcctggg cctcgccgcc accggagcca aggtcgtggt
     1501 catcgccggc aaccacgaca acccccgccg cctggccgcg gtcacgcccc tgctggcgct
     1561 ggctcaggtc acgctgctgg ccgagccggc gaaggctgac gccggcgggg tggtccattt
     1621 cgagacggcg gacggcgaga aggtcaacct ggccctgctg cccttcgtgt cccagcgggg
     1681 gatcgtcaag gcggacgacc tgatggccgg ccaggccgtc gaccactcgg ccacctacgc
     1741 cgaacgcctg cgccgcttgg tcgagcacct gaccgacggt ctcgacggcg aggacgccgt
     1801 gaacctgctg gccgctcacg ccatggtcag cggcggcgtc atgggcgggg gtgagcgcca
     1861 ggcccacacg atcttccagt acgagctgcc cggtgcggcc ttccccggct cgctgcacta
     1921 cgccgccctc ggccacctcc accggacgca ggcggtgccg ggtgcggcct gcccggcctg
     1981 gtacgccggg tccccgctgg ccctcgactt cggcgaggag accgatcgca aggccgtgct
     2041 ggtcgtcgag gcggagccgg gtgcccccgc cacggtgcgg ccggtcccgc tcgagtcggg
     2101 ccgcgccctg cgtacgttgc gcgggtcctt caccgacctc cgggagctgg cgagcgatgt
     2161 cggcgacgcc tggctgcggg tcgaggtcga cgagcccgct cgggccggcc tggccgagga
     2221 ggtgcgcgag ctcctccccc acgccgtaca ggtgtcggcc cggcccggcg aagccgccca
     2281 gggcggtgcc cgggctgccc ggctggggcg ttcgcccgac gagctcttcg ccgagtacct
     2341 ggccgagcag gacgtggaag acgaacggct gctggcgctc ttccgggagc tgcacgacga
     2401 gctcagcgac tcggggagtg ccgatgcggc ctgagcgcct ggaggtggag ggcttcaccg
     2461 cgttccggga gacggtcgcg gtcgacttcg acggggccga cctcttcgcc ttcaccggac
     2521 cgacgggctc gggcaagtcg agcctgatcg acgccatgac cttcgccctc tacggctcga
     2581 tcccgcgctt ggacaagaag gccgtcgcgc cggtcatcag ccagggcctc ctccaggccc
     2641 gggtccggct cgacttcacg gttggcgacg tcgggtacac cgcggtccgg atcgtccggg
     2701 ccaccagcgc gggcggcgcc accaccaagg aagcccggct ggagcggcgg gacgccgacg
     2761 gcggggtcga gacgatcgcc ggtgacgcca aggccctcac cacgcagatc gaggacctcc
     2821 tggggctctc cttcgagcac ttctgcacgt gcgtggtgct gccccagggg gagttcgccg
     2881 ctctcctcca cgccgagccc cgcaagcgtc aggacatgct cctggccctc ctcgatctcg
     2941 gcctctacga ggccatgggt caggccgccc gctcccgggc cacggcggcc gccgcccagg
     3001 tcgaggtcct cgaccgtcag gtggccgagc tggccgacgc ctccgaggag accctcaaag
     3061 aggccgaacg ccgggtgggc gtgctcgacc gtctcgtcga gcgcatcgac gaggcccgtc
     3121 cccggttgga ggagctggtc acggtcatcg ccgcggcgaa ggccgaggcc gaggaggccg
     3181 agcgccgcgt cgccaccctc accgacctgg ccgtgcccga cgacgtcgcc cggctggcgg
     3241 ccaaggccga cgccgcccgc aaggagctcg accaggcccg caaggaccac gaggcggcgg
     3301 ccgccttcct cgagcaggcc gaagccgcga ccggcgacct gcccgaccgg gtggccctgc
     3361 gggagcgcct gaccctgttg gcccgacggg ccgagcaggc cgagcgcatc cggaccggcg
     3421 cgaccctgga gggcgaacgg accgaggtgt tggccgccac ggtggccgtc cgtgaagaag
     3481 ccgacgccga ctacgccgag gcccaggtcg cggtggtcac ggccaaggac cgggaccggg
     3541 cggccgccat cgcccgggac ttggtggcgg gcgcgccctg tcccgtgtgc cgccaagtcg
     3601 tgcacgacgt gccgtcgcac agcgacgccc cggatctgac cgcagccgag aaggccctgg
     3661 ccgacaccac caaggcgatg cggaccgctc atgacgccga gcgggacgcc cagcgggagc
     3721 tggacaaggt gaccgcgctg ctgacggagg tccgccagac cgtgaccgat ctcgacgagc
     3781 agttgggcga gggcctcgac caggtcgcca tcgacgccgc cctggtcgac ctcgaccgac
     3841 gggaggccga cctgcgcacc gcccgcgaga cggagaagca gcgccgccgg gacctggagg
     3901 gggccgagcg ccgcgccgtc accctggccg acgacgagaa ggcgctctgg gtccgtttcg
     3961 acgaggctcg cgacgccttg gtcgccctcg gccccccggc cgccgcccga gaagatcttg
     4021 gggccgcgtg gggtgtcctg gtcgcctggg ccgacgaggc ccggcccgcc catgaggtcc
     4081 aggcggccga ggcccgggcc cgggtcgagc agtccgagac cgagcgcacg gccatcgagg
     4141 accgcatccg cgagacgtgc gccgagctgg acgtcacggt gggcgaccgg accccccggg
     4201 acgcggccac cgaggctcgg gagcgggccc gcacccgcgc cgaagggctg gccaaggacc
     4261 gtcagcgggc cgtcgagctg gaggcgcagc gccgcggcct ggtcgaacga cggcaggtgg
     4321 ccgaggaact gggtcgccac ctccgagccg accgcttcga gaagtggatc ctggatgagg
     4381 ccctccagcg cctggtcgag ggcgccgcgg acctgctgcg cgagctgtcg tcgggggcct
     4441 actcgctcac cttggacgcc ggtggggcga acttcgccgt cgtggaccac gccaacgccg
     4501 acgccgtgcg gggggccaag acgctgtccg gcggcgagac gttcctggcg tcgctggcgc
     4561 tggcgctggc tctggccgac caggttgccg acctggctgc cggcggcagc gcccgcctcg
     4621 agtcgatctt ctgcgacgaa ggcttcggtt ccctggacct cgacaccctg gacatcgtgg
     4681 ccacggccct ggaggagctg ggggcgtcgg gccggatggt cggggtggtc agccacgtcc
     4741 gcgagctggc cgagcgcctt cccgtccgct tcgaggtccg caagggcccc accacgagca
     4801 cggtcacccg ggtcgacgcg tgaccccaaa ttgcgaactt gcgtgggtca caccacagat
     4861 ccgtggtgcg atcgacgcaa gttcgaggtc gtcgtgaggc tggcggtcga gccttgggcg
     4921 ccggagtacg ggtcgccgac cgaggagggc gggttcacgc cccaggaccc gcccgttctg
     4981 gatctcgagc tcgcccccca ggactgggaa ccattgtccc cgggcggtgc gccggtcggc
     5041 accgtctact tcgtcgacgg cgtccagcgc atcgaggcca gcgtcgtggt cacgctggac
     5101 tccggcgagg cccgccgggg ccgctgcgtc tcctacggcg cgggggtcgt gcgttgcgac
     5161 ggccgggcca ccgtcgtgga ggcccaggct cgccacgagt tcctggcccc ggacagggcg
     5221 gcacaggaca ttcccacccg ccacggcacc ttcacccact acggggtcac ctcggacgac
     5281 tcgggcgtcc tggccaaggc cctgcacgac cggatggccg acctcgaggc cgaggtggct
     5341 cggtccgcgg cccccggcgc cgacgacctg ctcatcaccg acggtccgtt gagcggccgc
     5401 cggcacgggg tcaagcacgc cgtcggctac gtcaagaccc accctgtggc ctacctgccc
     5461 gaggatctgt cccagcgcgt cgtcaccgcg ctcctcccgg gccagcgcac gccggtcttc
     5521 cgcctcgggg ccggccgccc ggcctggtac gtccgcctgc ccacgccgat cgaccacggc
     5581 tggtcgggcg tcgtgcgctg cgaggcagag gacgaggtca gcggccccga gctcttcgcc
     5641 ctggccgacc gggtcaccgc caccctcccg aggttcgcca gcgagggcca caaggacccc
     5701 cgcgcccccc agaacctcta cccgatcggc gggctggaga aggagctcaa gcgccgcctc
     5761 ggcgaccccc gactctggtt ccgagccctc cgggaaaccg ccctccggtc cgacgcctga
     5821 gcccgcgtca ggggttcgtg ggggcggcgg cggccggggg ctcgtccatg gaggtccgga
     5881 ggtcggtgag gggcttgtcc gggaccttga tcgtgccccg ggtcgggcgg tcgaggtcga
     5941 aggtgaccag caggagcagg gcgaccaggg tggcggcgat cagcgccgga ccgatccccc
     6001 ggccgagcag ggccagcagg aacgccagga gggcgacggc gcaggctgcg gcgagcgcct
     6061 cgagggcgag cacggcgtca ggcacccggt tgttcaggac cgaggtccgg acggtctcct
     6121 ggtcgatcat ccggttcagg ctctcgacgt agagccggac ggcgctgcct tccggggact
     6181 ggtcgagcgc ctcgcccgcc aacgcccaca ggtcacgttg gagctcgtcg ccgtcggccg
     6241 cggccgcgtc gaaggccgcc gacgccggga tcgtgttcgc cagtcggatg ctggtgtcgg
     6301 cgtaccgccg gagcagctcg agggactcgg tccgctccgg ctcgggcagg gtctgggctc
     6361 gcaggtaggt ggtgccgatg gtgttggcgt cgtcgaccac ggcggcccgt cgggcgtcgt
     6421 agcgaccgac ggccagggcc aggccgaagg ccaggatgag ggccacgacg ccgaacagcg
     6481 aggcctgc
//
LOCUS       DASTFV010000366         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1346519, whole genome shotgun sequence.
ACCESSION   DASTFV010000366 DASTFV010000000
VERSION     DASTFV010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346519"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VFP02_06765"
     CDS             <1..727
                     /locus_tag="VFP02_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9542764.1"
                     /translation="NADALEAPPSAEHWFGGDDIGRDVFSRVIWGGRVSLFIGVAAVT
                     FGFVIGGTMGLTAGFYGGRYERVVIGAMDIMLAFPALVLALALVTFLSEPGTQNATLG
                     TVTLVLTILAVPALARITRAATLSFSQREFVTASRALGARNSRIIAREILPNVVPPMA
                     AFALIAIAIVIVAEGSLSFLGLSVASPTATWGNLIEQGRQGLDDTPWISLFPCAVMFL
                     TLLSFNYVGDQLRRYFDVKEVNL"
     gene            727..1815
                     /locus_tag="VFP02_06770"
     CDS             727..1815
                     /locus_tag="VFP02_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9542765.1"
                     /translation="MRKGRRQVVLARSDVDANVPLLEVVDLCTHFLTETGTVRAVDGV
                     SFTLNRGRTLGIVGESGSGKTILSRSIMGLLPPRNVVRTGSAKYEGTEVAGAGPEEMR
                     GLWGTEMAMVFQDPMTSLNPVMTVGNQITESLRYHLDMDKSEARETAIALLRSVGIPE
                     PAQRVKEYPHQLSGGMRQRVMIAIALACGPRLLLADEPTTALDVTVQAQILDLLQAQQ
                     TERHMAMILVTHDLGVVAGRADDIAVMYAGKVVEKAPTNVLFRSTRHPYTEALLKSIP
                     KLEHTSHTRLAVIPGRPPDLVSPPEGCNFSPRCPYAQPKCFVEEPVLEPGPGGGHEFA
                     CWFPVGTPEGAEALARNTQDASLSEGAA"
     gene            1815..>3160
                     /locus_tag="VFP02_06775"
     CDS             1815..>3160
                     /locus_tag="VFP02_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET9542766.1"
                     /translation="MAGTGTAHLRDPSETILRAEDLVVEFPVGRTGLKVHAVSGISLD
                     VKEGETLGLVGESGCGKSTTGRALMQFPSPTGGRVTFEGEELTALHGEDLRHIRTRMQ
                     MIFQDPISSLNPRRKVGDIVSEPLAVWSEPARPERGLMTTLAAILCFGMAVVFAVLAV
                     VYVFLAVILLTGAWANSATLGPITIKSAAVGFFIGFASAGGAVAFRAVGYGIWRRDPE
                     GRTEGITAFAAFAILGLVAWSVIGVSILVFLVPVTLLALSVVFLYGPGGSQDFPGVGP
                     EGQDLVDEVLTSVGLDPTVARAKRPHEFSGGQCQRISIARALVLDPKLIICDEPVSAL
                     DVSVQAQILNLLEDMKARYGLTLVFIAHDLAVVKNVSDRVAVMYLGKLCEVAPPDLLY
                     ERPAHPYTDALIKAIPVPDPEVRPDGVADKLQGDLPSPVAPPSGCHFRTRCPKAQDR"
BASE COUNT          444 a         1169 c         1031 g          516 t
ORIGIN      
        1 caacgccgac gccctcgagg ccccgccgtc cgccgagcac tggttcggag gggatgacat
       61 cggccgcgac gtcttctccc gggtcatctg gggtgggcgg gtctccctgt tcatcggggt
      121 cgccgccgtc accttcggct tcgtgatcgg ggggacgatg ggcctgaccg ccgggttcta
      181 cggcggacgc tacgaacgcg tcgtcatcgg ggccatggac atcatgttgg ccttccccgc
      241 cctcgtgctg gccctggctc tggtgacctt cctgtccgag cccgggaccc agaacgcgac
      301 gctgggcacg gtcaccctcg tcttgacgat cctggccgtt cccgcgctcg cgcggatcac
      361 gcgggcggcg acgttgtcct tctcccaacg cgagttcgtg accgcgtccc gcgccctcgg
      421 cgcccgcaac agccggatca tcgcccggga gatcctgccc aacgtcgtgc cccccatggc
      481 cgccttcgcc ctcatcgcca tcgccatcgt catcgtcgcc gagggctcgc tgagcttcct
      541 cgggctgtcg gtggcctcgc ccacggccac gtggggcaac ctcatcgagc agggtcgtca
      601 gggcctcgac gacacgcctt ggatctcgct cttcccgtgc gcggtgatgt tcctcacgct
      661 gttgtcgttc aactacgtgg gtgaccagct ccggcgctac ttcgacgtga aggaggtcaa
      721 cctctgatgc ggaagggtcg ccgtcaggtc gtgctggccc ggtccgacgt cgacgcgaac
      781 gtccccctgc tcgaggtcgt ggacctctgt acccacttcc tgaccgagac cggtaccgtc
      841 cgggccgtcg acggggtcag cttcacgctc aaccgggggc ggacactcgg gatcgtcggc
      901 gagtccggct ccggcaagac gatcctctcc cggtcgatca tgggcctgct gcctccccgg
      961 aacgtcgtgc ggacgggcag cgcgaagtac gagggcaccg aggtggccgg cgccgggccc
     1021 gaggagatgc gaggcctctg gggcaccgag atggccatgg tcttccagga cccgatgacc
     1081 tccctcaacc ccgtgatgac cgtgggcaac cagatcaccg agtcgttgcg ctaccacctc
     1141 gacatggaca agagcgaggc ccgggagacg gctatcgccc tccttcgctc cgtggggatc
     1201 cccgagccgg cccagcgggt caaggagtat ccccaccagc tctccggagg catgcgccag
     1261 cgcgtgatga tcgccatcgc cctggcctgc ggcccccgcc tgttgctggc cgacgagccc
     1321 accactgcgc tcgacgtcac ggtccaggcg cagatcctgg acctgctcca ggcccagcag
     1381 accgagcgcc acatggcgat gatcctggtc acccacgacc tcggcgtggt tgccggccgg
     1441 gccgacgaca tcgcggtgat gtacgcgggc aaggtggtcg agaaggcgcc gacgaacgtc
     1501 ctcttccgct cgacgcgaca cccctacacc gaggccctgc tgaagtcgat ccccaagctc
     1561 gaacacacca gccacacccg gctcgcggtg atcccggggc ggccccccga cctggtctcg
     1621 ccgcccgagg gctgcaactt cagcccgcgc tgcccgtacg cccaacccaa gtgcttcgtg
     1681 gaggagcccg tgctcgaacc cggtcccggc gggggccatg agttcgcctg ctggttcccc
     1741 gtgggcacgc cggagggggc cgaggccctg gcccgcaaca cgcaggacgc ctccctcagc
     1801 gaaggggccg cctgatggcc ggcaccggaa cggcccacct gcgcgacccg tccgagacga
     1861 tcctgcgggc cgaggacctg gtggtcgagt tccccgtcgg tcgcaccggc ctcaaggtcc
     1921 acgccgtctc ggggatcagc ctcgacgtga aggagggcga gaccctcggc ctcgtcggcg
     1981 agtccggttg cggcaagtcg acgaccggcc gtgccctcat gcagttcccc agtcccaccg
     2041 gtggtcgcgt caccttcgaa ggtgaggagc tgaccgccct gcacggggag gacctgcgcc
     2101 acatccgcac gcggatgcag atgatcttcc aggacccgat ctcctcgctc aacccgcggc
     2161 gcaaggtcgg cgacatcgtg tcggagccgt tggccgtctg gagcgagccg gcccggccag
     2221 agcgcgggct gatgaccacg ctggccgcca tcctgtgctt cgggatggcg gtggtcttcg
     2281 ccgtcctcgc cgtggtctac gtcttcctcg ccgtcatcct gctcaccggc gcgtgggcga
     2341 actccgccac gctgggcccg atcaccatca agagtgcggc cgtgggcttc ttcatcgggt
     2401 tcgcgtcagc gggcggagcg gtcgcgttcc gggcggtcgg ctacggcatc tggcgccggg
     2461 accccgaagg ccggaccgaa ggcatcaccg cgttcgcggc cttcgccatc ctcgggttgg
     2521 tggcctggtc ggtcatcggc gtctccatcc tcgtgttcct cgtgccggtc acgctcctgg
     2581 ccttgagcgt ggtcttcctc tacggccccg gaggcagcca ggactttccg ggcgtcggac
     2641 cggagggcca ggacctggtc gacgaggtgt tgaccagcgt cggcctcgat ccgaccgtgg
     2701 ccagggcgaa gcgaccccat gagttctccg ggggccagtg ccagcgcatc tccatcgccc
     2761 gcgccctggt gctggacccc aagctgatca tctgtgacga gccggtgtcg gccctggacg
     2821 tctcggtgca ggcccagatc ctgaacctgc tcgaggacat gaaggcccgg tacggcctga
     2881 ccctcgtgtt catcgcccac gacctggccg tggtcaagaa cgtcagtgac cgggtcgcgg
     2941 tgatgtacct cggcaagctc tgcgaagtcg ctcccccgga cctcctctac gagcggcccg
     3001 cccatcccta caccgacgcc ctcatcaagg ccatcccggt gccggacccc gaagtccggc
     3061 ccgacggggt cgccgacaag ctccagggtg acctgccgtc cccggtggcc ccgcccagcg
     3121 gctgccattt ccggacccgc tgccccaagg cgcaggaccg
//
LOCUS       DASTFV010000367         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1347898, whole genome shotgun sequence.
ACCESSION   DASTFV010000367 DASTFV010000000
VERSION     DASTFV010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347898"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFP02_06780"
     CDS             <1..217
                     /locus_tag="VFP02_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542767.1"
                     /translation="TCAFEPGAPPQGPADDDLGAEGVGVVVDDQSALITAAGDLATFT
                     VTNTFEADVDTPDVDPNVVAATPPFTG"
     gene            complement(229..1410)
                     /locus_tag="VFP02_06785"
     CDS             complement(229..1410)
                     /locus_tag="VFP02_06785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9542768.1"
                     /translation="MEFGMFLQGYVPGPAAHTTEGEHAALLNEVEMVVTADRHNWKYV
                     WVSEHHGLAEYSHTSSSEVFLGHCAALTERIHLGSGIFGLSPRANHPVRNAERVTMLD
                     HLSNGRFEFGTGRGAGSHEVATFNIHDTSSTRVEWDEVIWELPKMWERKDYTFQGEHF
                     AMDKPHNVLPKPYGVGHPPIWVACGSPSTFGKAGSLGIGALGFNFSPIDEMRPQIEAY
                     KSGIAGCSEPIGQFVNDNVMITNAVICLEDGDRAREVMTRAASGYLYSLVCLYHDTFP
                     VPEGAIVWPEPPRQLDRATVDLAIEYGGVLCGTPDEVNEQLSRYADVGVDQLVFGMPN
                     NLTQDETLECIELFGDKVIPNWDPDPVHRTTRMRQTAQPKYGPFEHEPPAIETIFTRG
                     D"
     gene            complement(1476..2168)
                     /locus_tag="VFP02_06790"
     CDS             complement(1476..2168)
                     /locus_tag="VFP02_06790"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET9542769.1"
                     /translation="MAKVGRPARISREAIAQAANDIGLSDLTLRAVADRLGVSIASLY
                     HHVEGKDDLLRLAAEYSAARTSIPQDEGQHWAVWLLEWALYNRDAFVAEPALFGQFMD
                     GAIGADVMADNTEAILARLVAQGFTAREAQSAYQLVSGCALGAAVQAIREQRAAAAGR
                     PAAKEYLRLLADREPGDLPHLRALLAEAAVTPLDSFETRITTVLAGIAARRGEDWSAI
                     VTKVDRLLTAHG"
BASE COUNT          327 a          741 c          856 g          368 t
ORIGIN      
        1 cacgtgcgcg ttcgagccgg gtgcgccgcc ccagggcccg gcggacgacg acctcggggc
       61 cgagggcgtc ggtgtcgtgg tggacgatca gtcggccctg atcaccgcgg ccggcgacct
      121 ggccacgttc acggtcacca acaccttcga ggccgacgtc gacacccctg acgtcgaccc
      181 gaacgtcgtc gccgccaccc cgcccttcac cggctgaccc gccgggactc agtccccgcg
      241 ggtgaagatg gtctcgatgg ccgggggctc gtgctcgaag gggccgtact tgggctgagc
      301 cgtctggcgc atgcgggtcg tgcggtggac cgggtccggg tcccagttgg ggatgacctt
      361 gtcgccgaac agctcgatgc actccagcgt ctcgtcctgg gtgaggttgt tgggcatccc
      421 gaagacgagc tggtcgacgc cgacgtcggc gtagcggctg agctgctcgt tgacctcgtc
      481 gggggtgccg cagaggacgc cgccgtactc gatggccagg tcgaccgtcg cccggtccag
      541 ctggcggggc ggctcgggcc agacgatggc gccctcgggc accgggaacg tgtcgtggta
      601 gaggcagacc agggagtaga ggtagccgct ggccgcccgg gtcatgacct cccgggcccg
      661 gtcgccgtcc tccaggcaga tgacggcgtt ggtgatcatc acgttgtcgt tgacgaactg
      721 gccgatgggc tcgctgcagc cggcgatgcc ggacttgtag gcctcgatct ggggccgcat
      781 ctcgtcgatg gggctgaagt tgaagcccag ggccccgatg ccgagcgagc ccgccttgcc
      841 gaaggtcgag ggggacccgc acgccaccca gatcggcgga tggccgacgc cgtagggctt
      901 gggcaggacg ttgtgaggct tgtccatggc gaagtgctcg ccctggaacg tgtagtcctt
      961 gcgctcccac atcttcggga gctcccagat gacctcgtcc cactcgaccc gggtggagga
     1021 ggtgtcgtgg atgttgaagg tggccacctc gtggctgccg gcaccccggc cggtgccgaa
     1081 ctcgaagcgc ccgttcgaga ggtggtcgag catggtgacc cgctcggcgt tgcgcaccgg
     1141 gtggttggcc cgcgggctga ggccgaagat gccggagccg aggtgaatgc gctcggtcag
     1201 ggcggcgcaa tggccgagaa agacctcgct ggaggaggtg tgggagtact cggccagacc
     1261 gtggtgctcg ctgacccaca cgtacttcca gttgtggcgg tcggcggtga ccaccatctc
     1321 gacttcgttc aggagagccg cgtgctcgcc ctcggtcgtg tgggccgcgg gcccggggac
     1381 gtagccctgc aagaacatgc cgaattccac cgggccatgg tggtcgattg tcgaccggtc
     1441 gtgcaagttc cgaccaccag atcggcgtgt gagggtcagc cgtgggcggt cagcaatcga
     1501 tcgaccttgg tgacgatcgc cgaccagtcc tcgccccggc gagcggcgat gccggccagg
     1561 accgtggtga tgcgggtctc gaagctgtcc agcggggtga ccgcggcctc agccagcagc
     1621 gctcgcaagt gggggaggtc gccgggctcg cggtcggcca ggaggcggag gtactccttc
     1681 gccgccggcc gccctgcggc tgcggcccgc tgttcgcgaa tggcctggac cgcggccccc
     1741 agcgcacagc cactgacgag ttggtaggca ctctgggcct cgcgggccgt gaagccctgg
     1801 gccaccaggc gggcgaggat ggcctcggtg ttgtcggcca tgacgtccgc accgatcgcc
     1861 ccgtccatga actggccgaa gagggccggc tcggccacga acgcgtcgcg gttgtagagc
     1921 gcccactcga gcagccagac ggcccagtgc tggccctcgt cctgagggat cgaggtgcgg
     1981 gcggcggagt actcggcggc cagccggagc aggtcgtcct tgccctcgac gtggtggtag
     2041 aggctggcga tgctgacccc gaggcgatcg gcgacggccc gcagcgtcag atcggagagg
     2101 ccgatgtcgt tcgccgcctg ggcgatcgcc tcacgactga tccgggccgg ccgccccacc
     2161 ttcgccacct cgtcagcgta cgacgcgctt gacaggacgc cgttcgtagg gttttctata
     2221 agagttcgga aatgggggac acgtgaacga gctggtcggc cgggtggccg tcgtgaccgg
     2281 tggcgccggg gg
//
LOCUS       DASTFV010000368         5558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1351582, whole genome shotgun sequence.
ACCESSION   DASTFV010000368 DASTFV010000000
VERSION     DASTFV010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351582"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1015
                     /locus_tag="VFP02_06795"
     CDS             143..1015
                     /locus_tag="VFP02_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9542770.1"
                     /translation="MNELVGRVAVVTGGAGGIGKALGEELLTEGMSLVVADVQADLVA
                     ATAAELGERGEVLGVVTDVSDPASVEALAAATYQHFGACHLLVNNAGVGAPSSTPWET
                     TVNDWRWVHGVNVMGVVNGVLSFVPRMIEAGEEGHVVNTSSGDGGIEPLPTASVYAAS
                     KAAVTTFTECLASQLESEGTKLRASIFYPSGGLLRTGLWTSDKTRPDELARERPRSTE
                     PMTVEKLEAMAEQGGYQLPWQDLNELARVLIAGIKNDDFIVMIGREKIADTLRTRGET
                     FAAGRLPVHEKGLL"
     gene            1012..2238
                     /locus_tag="VFP02_06800"
     CDS             1012..2238
                     /locus_tag="VFP02_06800"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9542771.1"
                     /translation="MSEDRYLLISSDGHTGPPADHYREYLAEAYHPQFDAHQAELGVI
                     REFQQSMNQDFRKEWEAKTGDGGLQAAYDSDARNERLDGEGVAAEVLFPDADVLGTGR
                     VASSPFGSGLGNPTGCDPELVFAGARAHNRWLADFCAKEPHRRIGVAVVPIVVDVDAA
                     VTEIHEVADRGLRGVMIPTRWFDSPAYHEPIYEPVWAAVAERGLVLHTHSGAGPADYE
                     IGPGFMAIYATEAYWWAARPLWVLLWAGVFERHPDLRYVIAENGAWWVPDLVTKMDEK
                     YLGGHNTRKMGDAFREQISMKPSEYIDRNCFFAASTPGVEDIGRRHAVGIGNLLWGND
                     FPHPEGTFPYTRELVRRRFKDVPHDECRAILGHNAVGLYGIDTAALAPLVERIGPLVE
                     DIHGDTPLQEVPEDRD"
     gene            2231..3307
                     /locus_tag="VFP02_06805"
     CDS             2231..3307
                     /locus_tag="VFP02_06805"
                     /inference="COORDINATES: protein motif:HMM:NF039286.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET9542772.1"
                     /translation="MTDVSELLKNPPSRTARESEARAGLTGLPMPRDAEMVTDPDRRL
                     KVTRQRGRRFPFPVPNGWFIVSRSDELDPGQVKALYLFGRDLVLYRTISGQPRVLDAH
                     CPHLGAHLAVGGKVEEECLRCPFHGWSFGPDGQCVDIPYSTSDHIPSKAHARTYPTVE
                     RNQMIWAWHHAEGEAPFYEVPEVEEFSSPDWTPIDCREFEIAVPCQDMAENNVDFAHF
                     RYVHGTDAIPEDDFFVDGTYKRTVGQDGNFVREGYGLGLGVLRIKGWVTFLSSTTPID
                     EENVLVRWVFTAPKANGEASVKEAADAFCSGVSQDIPIWENKIYRPRPVLTKSEKYIL
                     EHRRWSRQFYSNYDPSDEAELMED"
     gene            complement(3315..3797)
                     /locus_tag="VFP02_06810"
     CDS             complement(3315..3797)
                     /locus_tag="VFP02_06810"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9542773.1"
                     /translation="MATTDHETQIIVDPDVPLVRIIREFDAPPEKVFRAHADPELFAQ
                     WIGPDNMTARVDHFDCRTGGAWRYTMARDDFEASFHGSFHDVVPNELIVQTFTFDGVP
                     EGVALETLHLEDLGDGRTRLTATSLVDSFEGRDAVVASGMETGVIEGYNKLDALLAED
                     "
     gene            complement(3800..4159)
                     /locus_tag="VFP02_06815"
     CDS             complement(3800..4159)
                     /locus_tag="VFP02_06815"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9542774.1"
                     /translation="MPADPLSKVFSALADPTRRDMVARLTLADATVNELAEPYDVSVQ
                     AVSKHLKVLEGAGLVTRSREAQRRPVHLEAEVFDLMTKWIERYRRQAEERYQRLDALL
                     ATMPDGDPEPPDPGEKP"
     gene            complement(4253..5041)
                     /locus_tag="VFP02_06820"
     CDS             complement(4253..5041)
                     /locus_tag="VFP02_06820"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HET9542775.1"
                     /translation="MPVDEPWNMKPPRPHVEPSARWIRARVGETVLADSRRAMLLAWY
                     GPGRLPTYALPAEDVRTDRFTPSAGAGTEDFLVDHDIDADGRVLTRAARLFRHPPSPM
                     RGLDGHWTFTWDQGVAWFEEAMEVHVHARDTSKRVDVVPSERHVRVEVDGEVVADSGR
                     PHALFESWLPTRWYLPPVDVNEDLLVASDTTSDCPYKGHATYWSVQTATGLHPDVLWT
                     YADPVVECPRIAGLISFFNERVDLVVDGVLQERPLTPWSHGAPP"
     gene            complement(5054..>5558)
                     /locus_tag="VFP02_06825"
     CDS             complement(5054..>5558)
                     /locus_tag="VFP02_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9542776.1"
                     /translation="FVNLLVSAGTETVARLLGWAAMTLEAHPDQRAELAADAELLPNA
                     VDELLRYEAPSPVQGRWTTTETEFHGTTIPKDSKVLLLTGSAGRDERKYPDGERFDIH
                     RAIDTHVSFGYGIHFCIGAALARMEGRIALEEVLARFPEWQVDHDNATRLHTSTVRGW
                     INVPVRV"
BASE COUNT          850 a         1950 c         1924 g          834 t
ORIGIN      
        1 ccgatgtcgt tcgccgcctc ggcgatcgcc tcacgactga tccgggccgg ccgccccacc
       61 ttcgccaccc cgtgagcgta cgacgcgctt gacaggacgc cgttcgtagg gttttctata
      121 agagttcgga aatgggggac acgtgaacga gctggtcggc cgggtggccg tcgtgaccgg
      181 tggcgccggg ggcatcggca aggccctcgg cgaggagctg ctgaccgagg gcatgtccct
      241 cgtcgtcgcc gacgtacagg ccgatctggt cgcggccacc gccgccgagc tgggcgagcg
      301 gggcgaggtc ctgggcgtgg tcaccgacgt gtccgacccg gcatcggtgg aggccctggc
      361 cgccgccacc taccagcact tcggggcctg ccacttgctg gtcaacaacg cgggcgtcgg
      421 cgcgccgtcc tccaccccgt gggagaccac ggtcaacgac tggcgctggg tccacggcgt
      481 caacgtcatg ggcgtggtca acggcgtgct gtccttcgtc ccccggatga tcgaggcggg
      541 cgaggagggc cacgtggtca acacgtcctc cggcgacggg ggcatcgagc ctctgccgac
      601 ggcgtcggtc tacgccgcca gcaaggccgc cgtcaccacc ttcacggagt gcctggccag
      661 ccagctggag agcgagggga ccaagctgcg ggcctcgatc ttctacccat cgggcggcct
      721 gctgcggacc ggcctctgga cctcggacaa gacccgcccc gacgagctgg cccgggagcg
      781 accccgctcc accgagccga tgaccgtcga gaagctcgag gccatggctg agcagggggg
      841 ctaccagctg ccctggcagg acctcaacga gctggcccgg gtgctgatcg ccgggatcaa
      901 gaacgacgac ttcatcgtca tgatcgggcg cgagaagatc gccgacaccc tccgcacgcg
      961 gggtgagacg ttcgccgccg gtcggctgcc cgtccacgag aagggacttc tgtgagcgag
     1021 gatcgctacc tcctgatctc gtccgacggc cacacggggc cgccggcgga ccactaccgg
     1081 gagtacctcg ccgaggccta ccacccgcag ttcgacgccc atcaggccga gctgggcgtg
     1141 atccgagagt tccagcagtc catgaaccag gacttccgca aggagtggga ggccaagacc
     1201 ggcgacggcg gcctgcaggc cgcgtatgac tccgatgccc gcaacgagcg cctggacggc
     1261 gagggggtgg cggccgaggt cctcttcccg gacgccgacg tgctgggtac tggtcgggtg
     1321 gccagctcac ctttcggctc cggcctgggc aaccccaccg gctgtgatcc cgagctggtc
     1381 ttcgccgggg cccgggccca caaccgctgg ctggccgact tctgtgccaa ggagccccac
     1441 cgccgcatcg gcgtggccgt cgtgcccatc gtggtcgatg tcgacgccgc cgtgaccgag
     1501 atccacgagg tcgccgaccg gggcctgcgg ggcgtgatga tccccacccg ctggttcgac
     1561 tccccggcct accacgagcc gatctacgag cccgtttggg cggcggtggc cgagcggggc
     1621 ctcgtcctgc acacccactc cggcgcgggc cccgcggact acgagatcgg cccgggcttc
     1681 atggccatct acgccaccga ggcgtactgg tgggcggccc gtccgctctg ggtcctgctc
     1741 tgggccggcg tgttcgagcg ccaccccgac ctccgctacg tgatcgccga gaacggcgcc
     1801 tggtgggttc cggacctggt caccaagatg gacgagaagt acctcggcgg ccacaacacc
     1861 cggaagatgg gcgacgcctt ccgcgagcag atctcgatga agcccagcga gtacatcgac
     1921 cgcaactgct tcttcgccgc ttcgaccccg ggcgtcgagg acatcggccg gcgtcacgcg
     1981 gtcggcatcg gcaacctgct ctggggcaac gacttcccgc accccgaggg caccttcccc
     2041 tacacccgcg aactggtccg gcgccgcttc aaggacgtgc cccacgacga gtgtcgagcc
     2101 atcctgggcc acaacgccgt cggtctctac ggcatcgaca ccgccgcgct ggccccgctg
     2161 gtcgagcgca tcggccccct cgtggaggac atccacggcg acaccccact acaggaggtc
     2221 cccgaggacc gtgactgacg tatcggaact gctcaagaac ccgccctcgc gcaccgctcg
     2281 cgagtccgaa gcccgcgccg ggctcaccgg cctgcccatg ccccgggacg ccgagatggt
     2341 caccgacccg gaccgccgcc tgaaggtgac ccgccagcgg ggccggcgct tccccttccc
     2401 ggtccccaac ggctggttca tcgtctcccg gtccgacgag ctggatcccg ggcaggtcaa
     2461 ggccctctac ctcttcggcc gggacctcgt cctctaccgc acgatctcgg gccagccccg
     2521 cgtcctcgat gcccactgcc cccacctcgg cgcccacctg gccgtcgggg gcaaggtgga
     2581 ggaggagtgc ctgcgctgcc cgttccacgg ttggtcgttc gggcccgacg gccagtgcgt
     2641 cgacatcccg tactcgacca gcgaccacat cccgtccaag gcccacgccc gcacgtaccc
     2701 gaccgtcgag cgcaaccaga tgatctgggc ctggcaccac gccgagggcg aagcgccgtt
     2761 ctacgaggtc cccgaggtcg aggagttctc cagccccgac tggacgccga tcgactgccg
     2821 cgagttcgag atcgccgtgc cctgccagga catggccgag aacaacgtgg acttcgccca
     2881 cttccgctac gtccacggca ccgacgccat ccccgaggac gacttcttcg tcgacggcac
     2941 ctacaagcgc acggtcggcc aggacggcaa cttcgtacgg gagggctacg gcctcggcct
     3001 cggcgtcctg cgcatcaagg gctgggtcac cttcctgtcc tcgaccacgc ccatcgacga
     3061 ggagaacgtc ctcgtgcgct gggtcttcac cgcgcccaag gccaacggtg aggcctcggt
     3121 caaggaggcg gccgacgcct tctgcagcgg ggtcagccag gacatcccca tctgggagaa
     3181 caagatctac cggccccggc cggtgctgac gaagagcgag aagtacatcc tcgagcaccg
     3241 tcgctggagc cggcagttct actcgaacta cgaccccagc gacgaagcgg agctgatgga
     3301 ggactgaagg gcagtcagtc ctcggcgagg agggcgtcga gcttgttgta gccctcgatc
     3361 acgcccgtct ccatgccgct ggcgacgacg gcgtcgcggc cctcgaagct gtcgaccagc
     3421 gacgtcgccg tcagccgagt ccggccgtcg ccgaggtcct cgaggtgcag cgtctccagg
     3481 gccacgccct cgggcacccc gtcgaaggtg aacgtctgga cgatgagctc gttggggacg
     3541 acgtcgtgga acgagccgtg gaagctcgcc tcgaagtcat cgcgcgccat cgtgtagcgc
     3601 caggcgccgc cggtgcggca atcgaagtgg tcgacccggg ccgtcatgtt gtccggcccg
     3661 atccactgag cgaagagctc agggtcggcg tgggcccgga agaccttctc gggcggggcg
     3721 tcgaactcgc ggatgatccg cacgaggggg acgtcggggt cgacgatgat ctgggtctcg
     3781 tggtcggtcg tggccatggt cacggcttct ctcctgggtc gggtggttcg gggtcaccat
     3841 cgggcatcgt ggcgaggagg gcgtcgaggc gctggtagcg ctcctcggcc tggcggcggt
     3901 agcgctcgat ccacttggtc atcaggtcga agacctccgc ttccaggtgc accggtcggc
     3961 gctgggcctc ccggctccgg gtgacgaggc cggcgccttc gagcaccttc aggtgcttgg
     4021 agacggcctg gacgctgacg tcgtagggct cggccagttc gttcacggtg gcatcggcca
     4081 gcgtgagtcg agcgaccatg tcccgtcggg tgggatcggc cagcgccgag aacaccttcg
     4141 acagcggatc agccggcacc cgtcctcctc atgttcaacc gtttggttga agacaaccta
     4201 cgacgccgag cacctgttgt caaccaattg gttgacaaca cgcaggcgat gatcagggcg
     4261 gcgcgccgtg ggaccagggg gtgaggggtc gctcctggag gacgccgtcg acgaccaggt
     4321 cgacccgctc gttgaagaac gagatcaggc ccgcgatccg ggggcactcc accaccgggt
     4381 ccgcataggt ccagaggacg tccgggtgga gccccgttgc ggtctgcacc gaccagtagg
     4441 tggcgtggcc cttgtagggg cagtcgctcg tcgtgtccga cgccacgagc aggtcctcgt
     4501 tcacatcgac cggcggcagg taccaacggg tcggcagcca gctctcgaac agcgcatggg
     4561 gccggccgga gtcggcgacg acctcgccgt cgacctcgac ccgcacatgg cgctcgctgg
     4621 gcacgacgtc gacccgcttc gacgtgtccc gagcgtggac gtggacctcc atcgcctcct
     4681 cgaaccaggc cacaccctgg tcccaggtga acgtccagtg gccgtccagg ccgcgcatcg
     4741 gggacggcgg gtgccggaac aggcgggcgg cccgggtgag gacccggccg tcggcgtcga
     4801 tgtcgtggtc gaccaggaag tcttcggtgc cggcccccgc cgacggggtg aagcggtcgg
     4861 tccggacgtc ctcggcaggg agggcatagg tcggcaggcg tccagggccg taccaggcca
     4921 ggagcatggc ccgccggctg tcggccagga cggtctcacc cacccgggcc cggatccagc
     4981 gcgccgacgg ctcgacgtgc ggtcggggag gcttcatgtt ccacggctcg tcgacgggca
     5041 tcggcgtcgc cgatcagacg cggacgggga cgttgatcca gccccgcacc gtgctggtgt
     5101 gcagacgagt ggcgttgtcg tggtcgacct gccattcggg gaagcgggcc aggacctcct
     5161 ccagcgcgat gcggccctcc atccgggcca gggcggcccc gatgcagaag tggatgccgt
     5221 agccgaacga gacgtgggtg tcgatcgccc ggtggatgtc gaagcgctcg ccgtcggggt
     5281 acttgcgctc gtcccggccg gcggagccgg tcaggaggag gaccttgctg tccttgggga
     5341 tggtcgtgcc gtggaactcg gtctcggtcg tggtccaccg accctggacc ggtgacgggg
     5401 cctcgtagcg gagcagctcg tcgaccgcgt tgggcagcag ctcggcgtcg gcggcgagct
     5461 cggcccgctg gtccggatgg gcttccaggg tcatcgcggc ccagcccagg aggcgggcca
     5521 cggtctccgt gccggcgctg accaggaggt tgacgaag
//
LOCUS       DASTFV010000369         2571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1351697, whole genome shotgun sequence.
ACCESSION   DASTFV010000369 DASTFV010000000
VERSION     DASTFV010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351697"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VFP02_06830"
     CDS             <1..533
                     /locus_tag="VFP02_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9542777.1"
                     /translation="LVSGGRFVFGIGFGWNVEELADHGVAFADRRAVVAERIAAMKAL
                     WTEDEGGYTGKYVDFPPSFSWPKPVQKPHPPILLGGGGGPKLFEAIAAYGDGWMPIGG
                     AGVRKALPHLHAAMEAVGRDPAELTIIPFGVMPDPGKIDYYAEIGFDEVVFRIPPAPA
                     EKVLPVLDRLAEFVGT"
     gene            complement(543..899)
                     /gene="arsC"
                     /locus_tag="VFP02_06835"
     CDS             complement(543..899)
                     /gene="arsC"
                     /locus_tag="VFP02_06835"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00014.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HET9542778.1"
                     /translation="MSDEVVIFHNPSCSKSRQTLGILGDHGVEPEVVLYKEAPPDRAT
                     LEMILAKIDVEPSKLVRRDPEFKEAGLTPADVETAEQVVDVLLAHPALMERPVVVKGD
                     RVVLGRPPENVEALLD"
     gene            934..1512
                     /locus_tag="VFP02_06840"
     CDS             934..1512
                     /locus_tag="VFP02_06840"
                     /inference="COORDINATES: protein motif:HMM:NF036523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET9542779.1"
                     /translation="MADLSVADLTVSQTARRQRVIDAALSLGSAGGYEAVQMRDVAST
                     AGVALGTIYRYFTSKDHLLAAAMVDWTTDLERRTAQHPHRNGSTADAVVEVIRRATRP
                     MERDPRLAAAMVQAAAANEPSVTACQQQTTDIVERMLTAAMDGTDPERRARCARVLAH
                     VWNSSVLRWANGSGAVGQIGNDLEDAARLLLD"
     gene            1529..>2571
                     /locus_tag="VFP02_06845"
     CDS             1529..>2571
                     /locus_tag="VFP02_06845"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9542780.1"
                     /translation="MGDGFVVGLEHVLVLAVVDFDDTPEEAAFRAEARTWLEAHAERR
                     RPDARPSLAAANDPGHLEACRTWQRTLYDGGWAGITWPKAYGGRGGTANEARVFAQEE
                     RQFDVTTGMFSVAVNMIGPTLIAHGTPEQQERHLDAVLRGDEMWCQLFSEPGAGSDLA
                     GLRTRAVLDGETWVVNGQKVWSSGAHQADFGILLARTDPEATKHAGITYFLVDMTTPG
                     VEVRPLRQITGHAHFNEVFLTDVAIPEANVVGPVHGGWGVAQTTLASERSMIGGGGAA
                     TGIEQLVDLAREYGRTHEPGVRQDLARAYTRMRLLEFLGFRAQTALSQGKMPGPEVSV
                     MKLAISDHVERTG"
BASE COUNT          393 a          927 c          890 g          361 t
ORIGIN      
        1 acctcgtgtc cggcgggcgc ttcgtcttcg gcatcgggtt cggctggaac gtggaggagc
       61 tggccgacca cggcgtcgcc ttcgccgatc gccgggcggt ggtggccgag cgcatcgcgg
      121 ccatgaaggc gctgtggacc gaggacgaag gcggttacac ggggaagtac gtggacttcc
      181 cgccgtcgtt ctcgtggccg aaaccggtcc agaagccgca tccgccgatc ctgctcggtg
      241 ggggtggggg cccgaagctg ttcgaggcca tagccgccta cggcgacggc tggatgccca
      301 tcggtggggc cggcgtgcgc aaggcgttgc cccacctcca cgccgccatg gaggccgttg
      361 ggcgggaccc ggccgagctc acgatcatcc ccttcggggt gatgcccgat cccggcaaga
      421 tcgactacta cgccgagatc ggcttcgacg aggtcgtctt ccgcatcccc cctgcccccg
      481 ccgagaaggt cctgcccgtc ctcgaccgcc tcgccgagtt cgtggggacc tgagtgacca
      541 ggtcagtcca gcagggcttc gacgttctcc ggagggcggc cgaggacgac acggtcaccc
      601 ttgacgacga cgggacgctc catcaaggcg ggatgggcca ggagcacgtc gaccacctgc
      661 tcggcggtct cgacgtcggc gggggtgagg ccggcctcct tgaactcggg gtcgcggcgc
      721 accagcttcg acggctcgac atcgatcttg gccaggatca tctccagcgt cgcccggtcg
      781 ggcggggcct ccttgtagag gacgacttcc ggctcgacac cgtggtcccc gaggatcccc
      841 agggtctgcc gggacttcga gcagctcgga ttgtggaaga tcacgacctc atcgctcatt
      901 ccctgaacct accgaaaact agaatcggtt ccagtggctg acctgtccgt ggccgacctc
      961 accgtgagcc agacggcacg gcgccagcgg gtgatcgacg ccgccctcag cctcggctcg
     1021 gccggtggct acgaggccgt gcagatgcgg gacgtggcca gcaccgccgg ggtcgccctg
     1081 ggcacgatct accggtactt cacctcgaag gaccacctgc tggctgcggc catggtcgac
     1141 tggaccaccg acctcgagcg ccggacggcc caacacccgc accggaacgg ctcgacggcc
     1201 gacgccgtcg tcgaggtcat ccgccgggcc acccgaccca tggagcggga cccccgcctc
     1261 gccgcggcga tggtgcaggc cgcggccgcc aacgagccgt cggtgacggc ctgccagcag
     1321 cagaccaccg acatcgtcga gcgcatgctg accgcggcca tggacggcac tgacccggaa
     1381 cgacgggccc gctgcgcccg ggtcctggcc cacgtgtgga actccagcgt cctgcggtgg
     1441 gccaacggct ccggcgccgt cggccagatc ggcaacgatc tcgaggacgc ggcccgtctg
     1501 ctgctggact gatccgcccg ccacccgggt gggggacggg ttcgtcgtcg gactagaaca
     1561 cgttctagta ttggccgtcg tggacttcga cgacacgccg gaagaggctg ccttccgcgc
     1621 cgaagcccgg acctggctgg aagcccacgc cgagcggcgc cgacccgatg cccgcccatc
     1681 gttggccgcg gcgaacgacc ccggccacct cgaggcctgc cggacctggc agcgcaccct
     1741 ctacgacggc ggctgggccg gcatcacctg gcccaaggcc tacggcggtc gggggggcac
     1801 cgccaacgaa gcccgcgtgt tcgcccagga ggagcgtcag ttcgacgtca ccaccggcat
     1861 gttctccgtc gcggtgaaca tgatcggtcc caccctcatc gcccacggca ccccggagca
     1921 gcaggagcgc cacctcgacg ccgtgctgcg cggtgacgag atgtggtgcc agctgttcag
     1981 cgagccgggg gcgggctcgg atctggccgg gctgcgcacc cgggccgtgc tcgacggcga
     2041 gacgtgggtc gtgaacggac agaaggtctg gagctcgggc gcccaccagg ccgacttcgg
     2101 catcctcctg gcccgcaccg acccggaggc caccaagcac gccggcatca cctacttcct
     2161 ggtggacatg accaccccgg gcgtcgaggt ccggcccctc cgccagatca ccggccacgc
     2221 ccacttcaac gaggtcttcc tcaccgatgt ggccatcccc gaggcgaacg tggtgggacc
     2281 ggtgcacggc ggctggggcg tggcccagac gacgctggcc agcgagcggt cgatgatcgg
     2341 aggcgggggg gcggcgaccg gcatagagca gctcgtcgac ctggcccggg agtacggccg
     2401 cacgcacgag cccggcgtcc gccaggacct ggctcgggcc tacacccgca tgcgcctgct
     2461 cgagttcctc ggcttccggg cccagaccgc gctgagccag ggcaagatgc cgggccccga
     2521 ggtctcggtc atgaagctgg ccatctccga ccacgtcgag cgcaccgggg a
//
LOCUS       DASTFV010000370         3079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1354640, whole genome shotgun sequence.
ACCESSION   DASTFV010000370 DASTFV010000000
VERSION     DASTFV010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354640"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFP02_06850"
     CDS             <1..313
                     /locus_tag="VFP02_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HET9542781.1"
                     /translation="ISRGGETISAQEIEELLLGMDTVAEASVVAAPDERLGERAAAFL
                     RIHEGTTGPTLEQVRAHMEAAGLAPQKWPESLREVVDFPRTPSGKVQKYVLRQQLRED
                     P"
     gene            310..882
                     /locus_tag="VFP02_06855"
     CDS             310..882
                     /locus_tag="VFP02_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET9542782.1"
                     /translation="MTEPRIPPLPPDQWPEAMTEALAAIWPPNPRHPRPRREGRPKGL
                     NALGTLAQYPELARAYHVFNGHVLFATSLSLRQRELLVLRVAAIRQSDYEWKQHAVLG
                     ADVGLSDDEIARIAEGPDADGWPAIDAAMVRAVDELLADAKIRDTTWATLSEELDVHQ
                     LMDLVFTVGAYDALAMAFRSFGVELDEDLR"
     gene            1000..>3079
                     /locus_tag="VFP02_06860"
     CDS             1000..>3079
                     /locus_tag="VFP02_06860"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF002886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase"
                     /protein_id="HET9542783.1"
                     /translation="MDPTGEAPVVYLLHGTSAVEHRQLQKWVAERGGESTASDRPVET
                     VALDTGPDGTADGAALWALLERDDDPVLVPARVGWRPKERDGSRAARLQDVVLVGDPR
                     RPRPIAQEAILRRDPRRAEVVCGRPASVSELRALFDETGDGDGDTAAFGRFVARRATL
                     ALEREETRLLGPQYKVPRLVREEILSSNRFRTGAARLSAETGQSEAEVVRAATACLDE
                     MVAGYGRSQLDITLEIGRSISRHGYDPELDYDQDQAERMLAKVQGASVVLLPSHRSNF
                     DAVVIPMAWHDLGLPPTHTLAGDNMAFWPVGPIMRRSGSIFIRRDTKSDPVYRWVLRE
                     YVGYLVEKRFPLEWYVEGGRSRTGKMLPPKLGLLAYVVDAYLEGRTDDVVLVPASITY
                     DQLQEVADFASEARGQPKQKESLGWLVNSVRRSPRRYGTIYVRFGEPVSLRETLGPPV
                     DGTDLDPEERSLALNKLAFEVATRINQVTPITATALLTMALLAALGRAMTFAQVRTAV
                     RWQLADAQRRGLPLTSSARALETDGGLRSALTALADNEVVVAFDGGPDTVYLLGPDQH
                     LAAAFYRNSIIHHFLESAIAQLALVSAAEPDVADPLEAFWAAALRLRDLLKFEFFFRD
                     RASFRAALEAELDRDRPGWAECVAEGPDGIGALHPAIPLTAHILLRSFLEAYSVVALA
                     LEQRGSAPVKD"
BASE COUNT          419 a         1135 c         1078 g          447 t
ORIGIN      
        1 catcagccgc ggtggtgaga ccatcagcgc tcaggagatc gaggagctgc tgctgggcat
       61 ggacaccgtc gccgaggcca gcgtcgtcgc cgcgcccgac gagcgtctcg gggagcgcgc
      121 cgcggcgttc ctccggatcc acgagggcac gaccgggccg accctcgagc aggtacgggc
      181 ccacatggag gcggccggtc tggcccctca gaagtggccc gagtcactcc gggaggtcgt
      241 ggacttcccc cggacgccat cgggcaaggt ccagaagtac gttctccgcc agcagctccg
      301 ggaggatccg tgaccgagcc tcgcatccca ccgcttccgc ccgaccagtg gccggaggcg
      361 atgacggagg ccctcgccgc gatctggccg ccgaaccctc gccatccccg tccccgtcgt
      421 gagggccggc ccaagggcct gaacgccttg ggcacgctgg cccagtaccc cgagctggcc
      481 cgcgcctacc acgtcttcaa cggtcacgtc ctgttcgcca cctcgctgtc gctgcgtcag
      541 cgcgagctcc tcgtcctgcg cgtcgccgcc atccgccaga gtgactacga gtggaagcag
      601 cacgccgtgc tcggcgccga cgtcgggctg agcgacgacg agatcgcccg gatcgccgag
      661 ggtcccgacg ccgacggctg gcccgccatc gatgcggcca tggtccgcgc cgtcgacgag
      721 ctcctcgccg acgccaagat ccgcgacacc acatgggcga cgctgtccga ggagctcgat
      781 gtccaccagc tgatggacct ggtgttcacg gtgggggcct atgacgcgct ggcgatggcc
      841 ttccggtcct tcggggtcga gctcgacgag gacctccggt gagaacgctc ttctcattct
      901 gggagatccc tgctaccgtc cgttggatgc cacctcgatc cggagctggg acatgaccgg
      961 ctccgtgacc cgggcccgga tcgacggcgg ctgagggcca tggaccccac cggcgaggcc
     1021 ccggtcgtct acctgctgca cggcaccagc gcggtcgagc accggcagct ccagaagtgg
     1081 gtggccgaac gcgggggcga gtccacggcc tccgaccgcc ccgtcgagac ggtggcgctg
     1141 gacaccggcc cggacgggac cgccgacggt gcggcgctct gggccctgct cgagcgggat
     1201 gacgacccgg tgctcgtgcc cgcccgggtg ggttggcggc cgaaggagcg cgatggctcc
     1261 cgcgccgctc ggctccagga cgtcgtcctc gtcggcgacc cccgtcggcc tcgaccgatc
     1321 gcccaggagg cgatcctgcg ccgtgacccc cggcgggccg aggtggtgtg cggccggccg
     1381 gcgtcggtgt ccgagctgcg ggccctcttc gacgagaccg gcgacggcga cggtgacacg
     1441 gcggcgttcg gtcgcttcgt ggcccggcgg gcgacgctgg ccctcgagcg ggaggagacc
     1501 cgcctgctgg gaccgcagta caaggtgccc cggctcgtcc gggaggagat cctgtcgtcg
     1561 aaccggttcc ggaccggcgc cgcccggctg tcggccgaga cgggacagtc cgaggcggag
     1621 gtggtgcggg cggcgaccgc ctgcctcgac gagatggtcg ccggctacgg gcgctcccag
     1681 ctcgacatca ccctcgagat cggccgctcg atcagccgcc acgggtacga cccggagctc
     1741 gactacgacc aggaccaggc ggagcgcatg ctggccaagg tccagggggc gtccgtcgtc
     1801 ctcctcccca gccatcggtc gaacttcgac gccgtcgtca tcccgatggc ctggcacgac
     1861 ctcggcctgc ctcccaccca cacgttggcg ggggacaaca tggcgttctg gccggtcggg
     1921 ccgatcatgc gtcgctccgg ctccatcttc atccgccgcg acaccaagag cgacccggtg
     1981 taccgctggg tcctccggga gtacgtcggc tacctggtcg aaaagcggtt ccccctggag
     2041 tggtacgtcg agggcgggcg gtcacggacc ggcaagatgc tgccgcccaa gctcggcctg
     2101 ctggcctacg tggtcgacgc ctacctcgaa ggtcgcaccg acgacgtcgt gctcgtgccc
     2161 gcgtcgatca cctacgacca gctgcaggag gtggccgact tcgccagtga ggctcggggg
     2221 cagcccaagc agaaggagtc gctggggtgg ttggtcaact cggtgcgacg ctcgccgcgg
     2281 cgctacggga cgatctatgt ccgcttcggt gagccggtgt ccctccggga gacgttgggc
     2341 cccccggtcg acggcaccga cctcgacccc gaggagcgca gcctggcgct gaacaagctt
     2401 gcgttcgagg tggcgacccg catcaaccag gtcacgccca tcacggcgac ggccctgctc
     2461 acgatggcgt tgctggccgc gctcggccgg gcgatgacct tcgcccaggt ccgcaccgcg
     2521 gtccgttggc agctggccga tgcccagcgg cggggcctgc ccctgacgtc gagcgcccgg
     2581 gcgctggaga ccgacggtgg gctgcgctcc gcgctgaccg ccctggcgga caacgaggtg
     2641 gtcgtggcct tcgacggcgg cccggacacc gtctacctgc tcgggcctga ccagcacctc
     2701 gccgccgcgt tctaccgcaa ctcgatcatc caccacttcc tcgagagcgc catcgcccag
     2761 ctggcgctgg tcagcgcggc cgagcctgac gtcgcggacc cgctggaggc gttctgggcg
     2821 gcggccctgc gtcttcggga cctgctcaag ttcgagttct tcttccgcga ccgcgcctcg
     2881 ttccgggccg ccctcgaggc cgagctggac cgggaccgcc ccggctgggc cgagtgcgtc
     2941 gccgaggggc cggacgggat cggcgccctg caccccgcca tcccactgac cgcgcacatc
     3001 ctcctccggt ccttcctcga ggcgtacagc gtggtcgccc tcgccctcga gcagcgcggg
     3061 tcggcgccgg tgaaggatg
//
LOCUS       DASTFV010000371         4167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1354645, whole genome shotgun sequence.
ACCESSION   DASTFV010000371 DASTFV010000000
VERSION     DASTFV010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354645"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2293)
                     /locus_tag="VFP02_06865"
     CDS             complement(<1..2293)
                     /locus_tag="VFP02_06865"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HET9542784.1"
                     /translation="MGIAEGLDGKRIAVTGSTGFLGTALVERLLRSVPGCELVLLIRP
                     GRRSDPTRRANREIFRNDAFDRLREAWGDEFQAAIDRRVTAVAGDVGTDGLGLDDDGR
                     AAVATCDIVIHSAATVSFDSPLDQAVEVNLLGPSRIAAVLNDLGVTPHLVAVSTCYVA
                     GSRRGAAPEELVEESDFFTDVSWRGEVDAARRMRTDVEAASRTPEKLADYRKQARQEL
                     GAAGVPLLAEKTEQFRNRWVTDRMAEAGRARAASLGWPDAYAYTKALGERALCETRGD
                     LPVSIVRPSIIESAWAEPRPGWIRGFRMAEPVIISYARGLLKQFPGVPEGIVDVIPVD
                     QVAAAIIAVAGTGPSADGSVAITQVASGSANPLRYGQLVDLTSSWFKDHPLYDREGQP
                     IVPGDWSFPGRGRVESQLRRARTTLERADRVLSLLPLRGRQAEWSARLEDRREQAESA
                     IGYVELYGAYAECEAVYGVDNLLTRWDALDDGDRETFGFDPRTIHWPTYVQDIHLPSI
                     VTQARVRTEGGGRTTSDRQGRLREQVLSPDRHLAAFDLENTLIASNVVASYSWLASRR
                     LPPTDRARFVAQMLREGPSLLALDRRDRSDFLRHFYRRYENAPVDQIDADAWQMFSDL
                     ILTKSFPAGIRRVRRHRALGHRTVLITGALDFVVEPLRPLFDDIVCASLEQREGRYTG
                     GMKEVPPTGEARAQAMADYAKAEGLSLAESVAYADSASDLPMLEAVGHPVAVNPDAEL
                     AEIAKREGWEVLHFEKLGERLRMM"
     gene            2319..2918
                     /locus_tag="VFP02_06870"
     CDS             2319..2918
                     /locus_tag="VFP02_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9542785.1"
                     /translation="MTTSTRDRALDAALVSFATRGYEATSLDDVAGEVGVSKQAVLYY
                     FTSKPVLLDAVVDRSATELSAALEDALARAGEGWDRVEAVVRSVFRLALRRPELLGLL
                     REVSRLGPPAAPRLMAVLDPLVQRAREFLEVEMAAGRMRATDSRLLLMSAYSTVIGMA
                     TEVEVLRALGEEPTLRSLARRRRELLDFLRSALTAPVAA"
     gene            2915..3694
                     /locus_tag="VFP02_06875"
     CDS             2915..3694
                     /locus_tag="VFP02_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9542786.1"
                     /translation="MSWSKRPSVSFDRAAGYYDATRSLSPEAAAQVTAVLADALIGHG
                     PVLEIGVGTGRIALPLAEVGLDVVGIDLSMPMLRAMADKGGRVPIGQADCTQLPFPDD
                     RFGAVIASHVFHLVPEWRDATLEVLRVLRPDGALLWARGGLGARAKEVAVVFAKAAGI
                     DRAPVGLDQVEGLDAYLRSRGFVGDWLTEVPDDRLVSIDDLIDLIEAGVMGWTWSASD
                     EDRARGGEAARAWAAGQPEPSDEPRPIGLGVRFRRYVALPR"
     gene            3691..>4167
                     /locus_tag="VFP02_06880"
     CDS             3691..>4167
                     /locus_tag="VFP02_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542787.1"
                     /translation="MTATVTVLDPLLGAEGASAMLRLAEAFGTYRTYSAETTEQNYGD
                     LPQRIDAAANYVRSGGRFGRTGEPTSTLADRTNYFRETYAYGEDVRAPGIEPFLHHEG
                     FAEAARALHGRDVIVPAIVYANLLLPGQELAVHTDVPEFRGANRLHLPQWLMVVMHH"
BASE COUNT          557 a         1468 c         1542 g          600 t
ORIGIN      
        1 ccatcatccg cagccgctcg ccgagcttct cgaagtgcag aacctcccag ccttcccgct
       61 tggcgatctc cgccagctcc gcgtcgggat tgaccgcgac cgggtggccg acggcctcga
      121 gcatcggcag gtcggacgcc gagtcggcgt aggccacgga ctcggccagc gacagccctt
      181 cggccttggc gtagtcggcc atggcctggg ctcgggcctc gccggtcggg ggaacctcct
      241 tcatgccgcc tgtgtagcgg ccctccctct gctccagcga ggcgcagacg atgtcgtcga
      301 agaggggacg cagcggctcg accacgaagt cgagggcacc ggtgatgagc acggtgcggt
      361 ggccgagcgc ccggtgcctc cgcacccgcc ggatgccggc cgggaacgac ttggtcagga
      421 tcaggtcgct gaacatctgc caggcgtcgg cgtcgatctg atcgaccggc gcgttctcgt
      481 agcgccggta gaagtgacgc aggaagtcgc tgcggtcccg acggtcgagg gccaacaggc
      541 tcggcccctc ccgcagcatc tgggcgacga agcgggcccg gtccgtcggc ggcaggcggc
      601 gggaggccag ccacgagtac gacgcgacca cgttggaggc gatgagggtg ttctcgaggt
      661 cgaacgcggc caggtgccgg tccggggaca gcacctgctc ccgcagccgg ccctggcggt
      721 ccgaggtcgt gcgcccgccg ccttcggtcc gcacccgggc ctgggtgacg atcgacggca
      781 ggtggatgtc ctgcacgtac gtgggccagt ggatggtgcg gggatcgaag ccgaaggtct
      841 cccggtcgcc gtcgtcgagg gcgtcccagc gggtcagcag gttgtcgacg ccgtagacgg
      901 cctcgcactc ggcgtaggcg ccgtacagct cgacgtagcc gatggcgctc tcggcctgtt
      961 cccgccgatc ctcgagccgg gccgaccact cggcctgcct gccccgcagg ggcaggagcg
     1021 acaggacacg gtcggcccgc tccagtgtcg tcctggccct ccggagttgg gactcgaccc
     1081 gaccccgccc ggggaaggac cagtcgccgg ggacgatcgg ctgaccctcc cggtcgtaga
     1141 gcgggtggtc cttgaaccag ctcgaggtca ggtccaccag ctggccgtag cgcagggggt
     1201 tggccgagcc cgacgccacc tgcgtgatgg cgacggaccc gtcggcggac ggtccggtgc
     1261 cggcgacggc gatgatggcg gcggccacct ggtcgaccgg gatcacgtcg acgatgccct
     1321 cgggcacgcc ggggaactgc ttcagcaggc cccgggcgta ggagatgatc accggctcgg
     1381 ccatgcggaa gccccggatc caccccggcc tgggctcggc ccaggccgac tcgatgatcg
     1441 agggccgcac gatgctcacg gggaggtcgc cccgggtctc gcagagggct cgttcgccca
     1501 gcgccttcgt gtacgcgtag gcgtcgggcc aacccaacga cgccgctcgg gcccgcccgg
     1561 cctcggccat gcgatcggtg acccagcggt tccggaactg ctccgtcttc tcggccagca
     1621 ggggcacgcc ggcagccccg agctcctggc gggcctgctt gcggtagtcg gccagcttct
     1681 cgggggtccg gctggccgcc tccacgtcgg tgcgcatgcg gcgggcggca tcgacctcgc
     1741 cccgccagga gacgtcggtg aagaagtcgc tctcctcgac gagctcctcg ggagcggcgc
     1801 ctcggcggct gccggccacg tagcaggtgg agacggccac gagatggggc gtcacgccca
     1861 ggtcgttgag gacagcggcg atgcggctgg ggccgaggag gttgacctcc accgcctgat
     1921 cgagggggga gtcgaaggag acggtggcgg cggagtggat gacgatgtcg caggtggcca
     1981 cggccgcccg accgtcgtcg tcgagaccga ggccgtcggt gccgacgtcg ccggccaccg
     2041 ccgtgacccg tcggtcgatg gcggcctgga actcgtcccc ccacgcctcg cggaggcggt
     2101 cgaaggcgtc gttgcggaag atctcccggt tggcccgacg ggtgggatcg gagcgtcgcc
     2161 cggggcggat gagcaggacc agctcgcagc cgggcacgga gcgcaggagg cgctcgacca
     2221 gggcggtgcc gaggaagccc gtgctcccgg tgacggcaat gcgcttgccg tcgaggcctt
     2281 cggcgatgcc catggctcag tgttctacca tttggtaagt gaccacctcc acccgcgacc
     2341 gggcgctgga cgccgcattg gtgtccttcg cgacccgcgg ctacgaggcg acgtccctcg
     2401 acgacgtcgc cggcgaggtc ggcgtgtcca agcaggcggt cctctactac ttcacctcga
     2461 agccggtcct cctggacgcc gtcgtcgatc ggagcgcgac cgagctgtcg gccgcgctgg
     2521 aggacgccct ggcccgagcg ggggagggtt gggaccgggt cgaggccgtc gtccgctcgg
     2581 tgttccggct ggccctgcgt cggcccgagc tgctcggcct gctccgggag gtcagccgac
     2641 tcggtccgcc cgccgctccc cggttgatgg ccgtcctcga cccgctcgtg cagcgggccc
     2701 gcgagttcct cgaggtcgag atggccgccg ggcgcatgcg ggccaccgac tcccgcctcc
     2761 tgctgatgtc ggcctattca accgtcatcg gcatggccac ggaggtggag gtcctgcggg
     2821 ccctcggcga ggagcccacc ctgcgctccc tggcccgccg gcgccgcgag ctgctcgact
     2881 tcctccggtc ggcgctcacc gccccggtgg ccgcgtgagc tggtcgaagc ggccgtcggt
     2941 cagcttcgac cgggcggcgg ggtactacga cgccacccgt tcgctgtcgc cggaagcggc
     3001 ggcgcaggtg acggccgtgc tggccgatgc cctgatcggc cacggccccg tgctcgagat
     3061 cggggtcggc acgggccgca tcgccttgcc gctggccgag gtggggctgg acgtggtcgg
     3121 catcgacctg tcgatgccga tgctgcgggc catggccgac aagggcgggc gggtgccgat
     3181 cggccaggcc gactgcacgc agctgccgtt tcccgacgac cgcttcgggg cggtcatcgc
     3241 cagccacgtc ttccacctcg tacccgagtg gcgggacgcc acgctcgagg tcctccgggt
     3301 gctccgcccg gatggggcgc tgctctgggc ccggggtgga ctgggggccc gggccaagga
     3361 ggtggccgtg gtgttcgcca aggcggccgg catcgaccgg gcgccggtgg gcctcgacca
     3421 ggtggagggt ctcgacgcct acctgcgcag ccgaggcttc gtcggggact ggttgaccga
     3481 ggtgcccgat gaccgcctcg tgtcgatcga cgacctcatc gacctgatcg aagccggggt
     3541 gatgggctgg acctggtcgg ccagtgacga ggaccgggcc cgtggcggcg aggcggcacg
     3601 ggcgtgggcg gcgggacaac ccgagcccag cgacgagccc cgaccgatcg gcttgggggt
     3661 gaggttccgg cgctatgtag cgttgccccg atgacggcca cggtcaccgt gctcgacccg
     3721 ctcctggggg ccgaaggagc gtcggccatg ctccgattgg ccgaggcctt cgggacgtat
     3781 cgcacctaca gcgcagagac gaccgagcag aactacggcg acctgcccca gcgcatcgac
     3841 gccgccgcca actacgtgcg ctcgggcggc cgcttcggcc ggacaggcga gccgacgtcg
     3901 acgctggccg accgcacgaa ctacttccgt gagacctacg cctacggcga ggacgtgcgg
     3961 gccccgggca tcgagccctt cctgcaccac gaggggttcg cggaggcggc ccgtgccctg
     4021 catggacgcg acgtgatcgt accggccatc gtctacgcca acctgctgct ccccggccag
     4081 gagctcgccg tgcacaccga cgtgcccgag ttccgcgggg ccaaccggct ccacctcccg
     4141 cagtggctca tggtcgtcat gcaccac
//
LOCUS       DASTFV010000372         3572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1362376, whole genome shotgun sequence.
ACCESSION   DASTFV010000372 DASTFV010000000
VERSION     DASTFV010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362376"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFP02_06885"
     CDS             complement(<1..278)
                     /locus_tag="VFP02_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542788.1"
                     /translation="MTSSELDTAIAPRRRAAALGALLAGTAAVVTAIIGLRGDVGRAV
                     LAGIAVLLVVALMWTTTTRRGTTRRAAAVGLLVAIALLLVTVLTAEGHG"
     gene            complement(275..1987)
                     /gene="sulP"
                     /locus_tag="VFP02_06890"
     CDS             complement(275..1987)
                     /gene="sulP"
                     /locus_tag="VFP02_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HET9542789.1"
                     /translation="MSTTRHAVEAYVPGLRAVRTYERSWLSADVVAGLVLAAILVPQG
                     MAYAELAGLPPVTGLYTTVACLIGYAIFGPSRVLVLGPDSSISPLILAAITPLLVGSD
                     PAAAIALAGMLAIMVGLIEVGLGLGRLGFVADLLSTEVQVGYMNGLGITIIVGQLPKL
                     FGFSTDADSFFEELNAFVSGLDETDATTLIVGLCVLVVLLVLPHFTKRVPAVLVAVVG
                     ATLVSAVLDLAAEGVTTVGALPQGVPSPELPWTQASDLGPLLVAALGITLVSLTDTIA
                     TATSFAARRGDEVEPNQEMIGMGAANITAGLFQGFAVSTSGSRTAVAEQSGARSQLTG
                     VVGAGLVVVLLLFLNSLLADLPQTALAAVVISAALSLMDLGILRRYFQVRKSALALSL
                     VATAGVVFFGVLQGIVVAIVLAILMFFRRNWWPHGAVLGQVPGLRGWHSVDGHDDAVQ
                     IPGVVVYRWEAPLFFANAGAFRQQVRHLAREQQPSWIVLQCEAITDIDVTAADMLRQL
                     DDELNAQGIHLAFAELRSRLQEQALRYGLMETLDHDHFYPTLTMALDTVRAEGIDRRP
                     EEEP"
     gene            complement(1996..3387)
                     /locus_tag="VFP02_06895"
     CDS             complement(1996..3387)
                     /locus_tag="VFP02_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542790.1"
                     /translation="MAADEDPTPADGTAAADEEDVAALKAERDELAHEVEVLHARKKR
                     KGRVRGVLAVIGVVMSCILLVAACLGVFVRRSFLKTDNFSNRAGSLIDDPGVQTALSA
                     YLTEQLNQLIDPEQVLQDALPDRAQVLAVPLAGAVERFVGDQVSTFVASDTFARLWKD
                     AVELAHREVVRVLEGNTPLVEESKDHIVINLLPVINGVLAQISEASPEIFGRTVNLPT
                     VTVDDVPDAARQAIGNALGVTLDDNFGTFTVYDNGALSSAQEAVKVSDKLVWLLVVLA
                     PLAILATLLISVRRRRTLLQLTVGIVLSMILLRRLVFLFQKDLLDYVRIERNVPAVEA
                     TSNTFLDPLTTGALWLGIGALVVAAIAALTGPYGWAVRLRTGTVRLARLVGSAASGKA
                     EDPATAAWIAANLDKLRIAGAVVGVLLIWWIDVSWLGFFLLAALVGAYELALARLAER
                     AIPVEAEPVDATP"
     gene            complement(3387..>3572)
                     /locus_tag="VFP02_06900"
     CDS             complement(3387..>3572)
                     /locus_tag="VFP02_06900"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HET9542791.1"
                     /translation="AGPSEGRGTRLAIGGISLVIGVVVLVWPDATLLVLAILIGLRTL
                     LYGLLAIGIGVNVRRLG"
BASE COUNT          591 a         1155 c         1369 g          457 t
ORIGIN      
        1 ccatgaccct cggcggtgag aacggtgacg aggagcaacg cgatggcgac gaggagcccg
       61 acggcggccg cccgccgcgt cgttccccgc cgcgtcgtcg tcgtccacat cagggcgacc
      121 accagcagga cggcgatgcc ggcgagcacg gcgcgcccga cgtcaccccg caggccgatg
      181 atggcggtga ccaccgcggc ggtgccggcc aggagcgcgc cgagggcggc cgcgcggcgg
      241 cgaggggcga tggcggtgtc gagttcgctg cttgtcatgg ttcctcctcg ggccggcggt
      301 cgatgccctc ggcgcggacc gtgtccagcg ccatggtgag ggtcgggtag aagtggtcgt
      361 ggtccagcgt ctccatcagc ccgtagcgga gggcctgttc ctgcagccgg ctgcggagct
      421 cggcgaaggc gaggtggatc ccctgggcgt tgagctcgtc gtccagctgg cggagcatgt
      481 ccgccgcggt gacgtcgatg tcggtgatcg cctcgcactg caggacgatc cacgagggct
      541 gctgctcacg ggccaggtgg cggacctgtt ggcggaacgc cccggcgttg gcgaagaaca
      601 ggggcgcctc ccaccggtag acgacgacac cggggatctg gacggcgtcg tcgtggccgt
      661 cgacactgtg ccaacccctg aggccgggga cctggccgag gacggcgccg tggggccacc
      721 agttgcgccg gaagaacatc aggatggcga ggacgatggc cacgacgatg ccctgcagca
      781 cgccgaagaa gacgacgccg gcggtggcga ccagagacag ggcgagcgcg ctcttgcgca
      841 cctggaagta gcgccggagg atcccgaggt ccatgaggga cagcgcggca gagatgacca
      901 cggcggcgag ggccgtctgg gggaggtcgg cgagcaacga gttgaggaag agcaggagga
      961 ccacgaccag gcccgcgccc acgaccccgg tcagctggct gcgggcgccg gactgctccg
     1021 ccaccgccgt gcgggagccg ctggtcgaca ccgcgaaccc ctggaagagg cctgcggtga
     1081 tgttcgccgc ccccatcccg atcatctcct ggttgggctc gacctcgtcg ccgcgccgag
     1141 ctgcgaagga ggtggcggtg gcgatcgtgt ccgtgaggga gacgagggtg atccccaagg
     1201 cagccacgag gagtggcccg aggtcgctgg cctgggtcca gggcagctcc ggtgacggca
     1261 ccccctgggg cagggcgccg acggtggtga ccccctccgc ggccaggtcg aggacagccg
     1321 agacgagcgt ggccccgacc acggccacga ggacggctgg tacccgcttg gtgaagtggg
     1381 gcaacacgag gaggacgacg aggacgcaga gcccgacgat gagggtcgtg gcatcggtct
     1441 cgtcgaggcc ggagacgaag gcattgagct cttcgaagaa cgagtcggcg tcggtggaga
     1501 agccgaagag cttcggcaac tggcccacga tgatggtgat gccgaggccg ttcatgtacc
     1561 cgacctggac ctcggtggag agcaggtcgg cgacgaaccc gagccgaccg agtcccaggc
     1621 cgacctcgat gagcccgacc atgatggcca gcatcccggc cagggcgatg gcggcagccg
     1681 ggtccgagcc cacgagcagc ggggtgatgg cggcgaggat caggggcgag atcgacgaat
     1741 ccgggccgag gacgagcacc cgtgacgggc cgaagatggc gtaaccgatg aggcaggcca
     1801 cggtggtgta gagcccggtg accggaggaa gcccggccaa ttcggcgtag gccatgccct
     1861 gggggaccaa gatcgcggcc aggaccagcc cggccacgac atcggcggag agccaggatc
     1921 gctcgtaggt gcggaccgct cgaaggccgg ggacgtaggc ctcgaccgcg tgtcgcgtcg
     1981 tactcacggc ccgggtcagg gggtggcgtc gaccggctcg gcctcgacgg ggatcgcccg
     2041 ctccgccaac cgggccagcg cgagctcata ggccccgacg agagcggcca gcaggaagaa
     2101 ccccagccag gagacgtcga tccaccagat cagcaggacg ccgacgacgg cgccggcgat
     2161 gcggagcttg tcgaggttgg ctgcgatcca cgccgctgtg gcggggtcct cggccttgcc
     2221 ggaggcggcg gagcccacca accgcgcgag tcggacggtg ccggtccgga gtcggacagc
     2281 ccagccgtag gggcccgtga gggcggcgat ggccgcgacg accagggcgc cgatgccgag
     2341 ccacagcgca ccggtggtca gcgggtcgag gaaggtgttg ctcgtcgcct cgaccgccgg
     2401 aacgttgcgc tcgatgcgca cgtagtcgag caggtccttc tggaacagga agaccaggcg
     2461 gcggagcagg atcatgctca ggacgatgcc gacggtgagc tgcaggagcg tccgccgccg
     2521 tcgtaccgag atgagaagcg tggccaggat ggccaggggg gccagcacca ccaggagcca
     2581 gaccagcttg tcggacacct tcactgcctc ctgggcggag ctgagggccc cgttgtcata
     2641 gacggtgaac gtgccgaagt tgtcgtccag ggtgacgccg agggcgttgc cgatggcctg
     2701 acgggcggcg tccggtacgt cgtcgacggt gaccgtcggc aggttgacgg tgcgaccgaa
     2761 gatctcgggg gaggcctcgc tgatctgggc cagcaccccg ttgatcaccg ggagcaggtt
     2821 gatgacgatg tggtccttgc tctcctcgac cagcggcgtg ttgccctcga gcacgcggac
     2881 gacctcgcgg tgggcgagct ctacggcgtc cttccagagc cgggcgaagg tgtccgacgc
     2941 gacgaaggtc gagacctggt cgccgacgaa gcgctcgacg gcgccggcca gcgggacggc
     3001 gaggacctgg gcccggtcgg gcagggcgtc ctggaggacc tgctcggggt cgatgagctg
     3061 gttcagctgc tcggtcaggt acgcgctcag ggcggtctgg accccggggt cgtcgatcag
     3121 gctccccgcc cggttgctga agttgtcggt cttcagaaag ctcctgcgga cgaacacgcc
     3181 caggcaggcg gcgaccagca ggatgcagga catcaccacg ccgatcacgg cgaggacgcc
     3241 ccggacccgg cccttgcgct tcttgcgggc atggaggacc tcgacctcgt gggccagctc
     3301 gtcccgctcc gccttcaacg cggccacgtc ctcctcgtcg gcggccgccg tgccgtcggc
     3361 gggtgtcggg tcctcgtcgg cggccatcag ccgagccgcc gcacgttgac gccgatcccg
     3421 atggcgagca ggccgtagag caaggtgcga agcccgatca ggatcgccag caccagcagg
     3481 gtggcgtccg gccagaccag cacgacgacg ccgatgacga ggctgatccc gccgatggcg
     3541 agccgggtgc cgcggccttc ggacgggccg gc
//
LOCUS       DASTFV010000373         2936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1363560, whole genome shotgun sequence.
ACCESSION   DASTFV010000373 DASTFV010000000
VERSION     DASTFV010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363560"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..517
                     /locus_tag="VFP02_06905"
     CDS             32..517
                     /locus_tag="VFP02_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET9542792.1"
                     /translation="MVEMELVGVRVELPTNTPIVLLRETGGDRRMLPIFIGAPEATAI
                     AFALEQVPTPRPMTHDLVKNLLDEVGVTLERIVVTDLQDSTFFAELHLVQGEDTCQLS
                     SRPSDAIALAARTGSPIFAEEAVLDEVGYTVREEAQPEEEVVEEFRRFIDNIDPEDFA
                     S"
     gene            529..1530
                     /locus_tag="VFP02_06910"
     CDS             529..1530
                     /locus_tag="VFP02_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HET9542793.1"
                     /translation="MAPSGDPWRRVQLGCLLLVATILVGTGGYLILGLSPLDALYQTV
                     TTISTVGFREMPADPSTGFRVFTMVLILTGVGVALYTASVLLESVVEGRLTERFGRRR
                     MQRTIDELSGHVILCGWGRVGRSIERHLRGAGVDVVVVEADADRCDAVSGLSVCGDAT
                     EDEVMTRAGLDRAATLVAAVDSDAANLFITLSARDRRPDLFIVSRVRDEANEAKLRQA
                     GSDRVVNPQQIGGARIAALALSPNVADFLDVVMHDGSLEFRLADIAVPEASSLAGQSL
                     RAAHVRDETGALVLAIRAQDGDFTTNPPPEIVLRQGDVLIAIGTEEQLDDLRRMAGR"
     gene            1624..2118
                     /locus_tag="VFP02_06915"
     CDS             1624..2118
                     /locus_tag="VFP02_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9542794.1"
                     /translation="MTGQQEMFPRHVGYGGKKAAEIVGISYRQLDYWARTDLVRPSLV
                     DAAGSGSRRKYSYQDLLELKVIKSLLDAGIRLDLVREVFTYLRDQLGEDPASANLVIS
                     GEHTVLTRDGEEMIDLVRKGQGVLNILPLSGVVEEVDAAIVQLFPEAPATPAGSSVEV
                     AAEQ"
     gene            2115..2462
                     /locus_tag="VFP02_06920"
     CDS             2115..2462
                     /locus_tag="VFP02_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542795.1"
                     /translation="MSVGAPHESVDFAHPSERDFARVLDFYGVEWRYEPRTFVLHENA
                     DGHPTEAFSPDFYLPDLDLYIELTTLKQRLVTKKNRKIRELRQRYPGINVMILYRKDW
                     QNLAVKYGMDSAA"
     gene            2494..>2936
                     /locus_tag="VFP02_06925"
     CDS             2494..>2936
                     /locus_tag="VFP02_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HET9542796.1"
                     /translation="MNLRLSPLDAAHRALGAKMVPFGGWDMPLSYGDGTIAEHRACRT
                     GAVAFDVSHLGTVRVTGPEAKGLLQANLTADLDKIEPGRTQYTHLLDADDASVLDDII
                     VWWLGEDRFDVMPNASNTAGVVAAIGGVDVTPTRAIIAVQGPRAR"
BASE COUNT          458 a         1042 c         1004 g          432 t
ORIGIN      
        1 ggcgagctct atccgtaggt aggttgtggc catggtggag atggagctcg tcggtgtccg
       61 ggtggagctg cccaccaaca cccccatcgt gctcctgcgg gagacgggcg gtgatcgccg
      121 gatgctgccg atcttcatcg gcgctccgga ggccacggcc atcgccttcg ccctggagca
      181 ggtgccgacg cctcgaccca tgacccacga cctggtgaag aacctgctcg acgaggtcgg
      241 ggtgaccctg gagcggatcg tggtcaccga cctgcaggac agcacgttct tcgccgagct
      301 gcatctcgtg cagggcgagg acacctgcca gctctcgagc cggccgtccg acgccatcgc
      361 gctggccgcc cgcacgggct cgccgatctt cgccgaggaa gcggtcctcg acgaggtggg
      421 ctacacggtg cgggaagagg cccagcccga ggaggaagtg gtcgaggagt tccgtcgctt
      481 catcgacaac atcgaccctg aggacttcgc gtcctgaccg cgccccggat ggcgccgtcg
      541 ggtgacccct ggcgccgggt gcagctgggt tgcctcctgc tggtcgccac catcctggtg
      601 ggcaccggcg gctacctgat cctgggcctc agcccgctcg acgccctcta ccagacggtc
      661 accaccatct cgacggtggg attccgggag atgcccgccg acccctcgac cgggttccgg
      721 gtgttcacca tggtcctgat cctgaccggg gtcggggtgg ccttgtacac ggcatcggtg
      781 ctgttggagt cggtggtcga gggccggctc acggaacgct ttgggaggcg acgcatgcaa
      841 cggaccatcg acgagctctc gggccacgtc atcctctgtg gctgggggcg ggtgggtcga
      901 tccatcgaac gccacctccg gggagccggc gtggacgtgg tcgtggtgga ggccgacgcc
      961 gaccgctgtg acgcggtgtc cgggctgtcg gtgtgcgggg acgccaccga ggacgaggtc
     1021 atgacccgag ccgggctgga tcgggcggcc accctggtgg cggcggtcga cagcgacgcc
     1081 gccaacctct tcatcacctt gagcgcccgt gaccggcgcc ccgacctgtt catcgtcagc
     1141 cgggtgcggg acgaggccaa cgaggccaag ctgcgccaag cggggtccga ccgggtggtc
     1201 aacccccaac agatcggcgg ggcccggatc gccgccctcg ccctgtcccc gaacgtggcc
     1261 gacttcctcg acgtcgtgat gcacgacggc agcctggagt tccgtctggc cgacatcgca
     1321 gtgcccgagg cctcgtccct cgccggccag agcctgcggg ccgcccacgt ccgggacgag
     1381 acgggggcgc tggtcctggc catccgggcc caggacggtg acttcaccac caacccaccc
     1441 cccgagatcg tgctgcggca gggggacgtg ctcatcgcca tcggcaccga ggagcagctc
     1501 gacgacctcc ggcggatggc gggtcgttga cctcaacgtg aggtcgggcg tactgtgacg
     1561 gcgtcacacc cgcgtaacta cgcgcgtgtg atggggcggg cgcccgcgag aggacaccac
     1621 cacatgaccg gacagcagga gatgttccct cgccacgtcg gctacggggg caagaaggcg
     1681 gccgagatcg tcggcatcag ctaccgccag ctggactact gggcgcggac cgacctggtc
     1741 cgaccgtccc tggtcgacgc cgccggcagc ggcagccgcc gcaagtactc ctaccaggac
     1801 ctgctcgagc tcaaggtgat caagagcctg ctcgacgccg gcatccgcct cgacctggtg
     1861 cgggaggtct tcacctacct gcgcgaccag ctcggcgagg acccggccag cgccaacctg
     1921 gtgatcagcg gtgaacacac cgtgctcacc cgggacggcg aggagatgat cgacctggtc
     1981 cgcaagggcc agggcgtgct caacatcctg ccgctgtcgg gcgtggtgga agaggtcgac
     2041 gccgccatcg tccagctgtt ccccgaggcc ccggcgacgc cggccggatc gtccgtcgag
     2101 gtcgcggccg agcagtgagc gtcggggcgc cccacgagtc ggtggacttc gcccacccct
     2161 ctgagcggga cttcgcccgg gtgctggact tctacggcgt cgagtggcgc tacgagcccc
     2221 gcaccttcgt gctccacgag aacgccgacg ggcaccccac ggaggcgttc agccccgact
     2281 tctacctgcc cgacctcgac ctctacatcg agttgaccac cctcaagcaa cggctggtca
     2341 ccaagaagaa ccgcaagatc cgcgagctgc gccagcgcta cccggggatc aacgtgatga
     2401 tcctctaccg caaggactgg cagaacctcg ccgtcaagta cggcatggac tcggccgcgt
     2461 agatcgcacc gggccgctcc ttagaatctc ggggtgaatc tccggctctc gccgctcgac
     2521 gcggcccatc gcgccctcgg ggcgaagatg gtgcccttcg gcggctggga catgcccctg
     2581 agctacggcg acggcacgat cgccgagcac cgggcctgcc gcaccggcgc ggtcgccttc
     2641 gacgtcagcc acctcggcac cgtccgggtc acgggcccgg aggcgaaggg cctgctccag
     2701 gccaacctca ccgccgacct ggacaagatc gagccggggc ggacccagta cacgcacctg
     2761 ctcgacgccg acgacgcctc ggtcctcgac gacatcatcg tgtggtggct gggcgaggac
     2821 cgcttcgacg tcatgcccaa cgcctccaac acggcaggcg tggtggccgc catcggcggg
     2881 gtcgacgtga cgcccacccg ggccatcatc gccgtgcagg ggccccgggc ccgcga
//
LOCUS       DASTFV010000374         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1364165, whole genome shotgun sequence.
ACCESSION   DASTFV010000374 DASTFV010000000
VERSION     DASTFV010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364165"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VFP02_06930"
     CDS             <1..563
                     /locus_tag="VFP02_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET9542797.1"
                     /translation="LLSMGNARQDLLERVMDDVAHHGMGGRSLREMAEAVGSSHRMLL
                     YHFGSRDGLVAAVVEQVEAQQRGALAEAARTAPPGGQPEDVVLALWHQVASEELRPFV
                     RLFFEALASMGLEGGDGDLTSAWLDESEAVGRLLGTPADSVDLRLGVAVVRGLLVDVV
                     ATGDTGPATEALVRYLDLWRAGRVTT"
     gene            596..1030
                     /locus_tag="VFP02_06935"
     CDS             596..1030
                     /locus_tag="VFP02_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET9542798.1"
                     /translation="MAIEGTYEPSPWTPVADQVRRYEESGGTEGTLLEGQPCVILWTR
                     GRHSGTVRKTPLMRVTDGERYAVVASLGGAPQHPVWYHNVVADPEVSLQDGPDLRDYT
                     AHVASAGEKAEWWPRATATWPAYDDYQASTDRDIPVIVLEPH"
     gene            complement(1046..>2095)
                     /locus_tag="VFP02_06940"
     CDS             complement(1046..>2095)
                     /locus_tag="VFP02_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET9542799.1"
                     /translation="IIMRAVVYHGPGRKAWEDVPKPEIIEDTDAVVRVDAVTICGTDL
                     HILKGDVPAVTDGRILGHEAVGTVESVGAGVKNVAVGDHVLVSCITACGACRYCREGS
                     YSQCLGGGGWILGHKIDGTQAEYVRVPFADTSTYVVPDGVADEDILMLADILPTGYEV
                     GVLNGKVRPGDVVAVVGAGPIGLAAMIGSRLFSPAHVVAIDLADSRLDAAKQFGADIV
                     VNNGREDPLAVVRELTGGLGADVAIEAVGVPDTFELATRLARPGGHIANIGVHGTPAT
                     LHLEELWIRNVTITTGLVDTYSTPTLLRLAQGKQVDAGRFVTHHFTLDQFEEAYEVFG
                     NAADTGALKVVLTRA"
BASE COUNT          301 a          730 c          760 g          304 t
ORIGIN      
        1 acttgctgtc catggggaat gcccgccagg acctgctcga acgggtgatg gacgacgtcg
       61 cccaccacgg gatgggcggg cgcagcctcc gggagatggc cgaggccgtg ggctccagcc
      121 accgaatgct gctctaccac ttcggttcac gcgacggtct ggttgccgcc gtggtcgagc
      181 aggtcgaggc ccagcagcgg ggcgcccttg ccgaagcggc ccggacggcg cccccggggg
      241 gccagcccga ggacgtggtc ctggctctct ggcaccaggt cgcctccgag gagttgcgcc
      301 cgttcgtgcg cctcttcttc gaggcgctcg cctccatggg cctggagggg ggggacggcg
      361 acctgacctc ggcctggctg gacgagagcg aggcggtcgg gcgactcctg gggacccccg
      421 cggacagcgt cgacctgcgc ctgggtgtcg ccgtcgtgcg cggcctccta gtggacgtcg
      481 tcgccaccgg cgacaccggg ccggcgaccg aggccctcgt ccgctacctc gacctgtggc
      541 gagccggccg ggtgacgacg tagggccgat accgccgccg ggatagacac ggagcgtggc
      601 gatcgaggga acctacgaac ccagcccgtg gaccccggtg gcggaccagg tccgtcgcta
      661 tgaggagtcc ggcggcaccg agggaacgct gctcgagggc cagccctgcg tgatcctctg
      721 gacccgcggc cggcacagcg gcaccgtgcg caagacccct ctgatgcggg tgaccgacgg
      781 cgagcgctac gcggtggtgg cctcgttggg aggtgccccg cagcatccgg tctggtacca
      841 caacgtggtc gccgaccccg aggtgagcct gcaggacggc cccgacctcc gggactacac
      901 cgcccacgtc gccagcgccg gggagaaggc cgagtggtgg ccccgggcca ctgcgacctg
      961 gcccgcctac gacgactacc aggcgtcgac cgaccgcgac atcccggtca tcgtcctcga
     1021 gccgcactga ccggccggcg aggattcagg ctcgggtcaa gacgaccttc agcgccccgg
     1081 tgtcggcggc gttgccgaag acctcgtagg cctcctcgaa ctggtcgagg gtgaagtggt
     1141 gggtgacgaa gcggccggcg tccacctgct tgccctgcgc caggcggagc agcgtcggcg
     1201 tggagtaggt gtccacgagc cccgtggtga tcgtgacgtt gcggatccac agctcttcga
     1261 ggtgcagcgt ggccggggtg ccatgcaccc cgatgttggc gatgtggccc ccgggccggg
     1321 ccagccgggt ggccagctcg aaggtgtcgg gcacgccgac ggcctcgatg gcgacgtcag
     1381 ctcccagccc gccggtcagc tcgcgcacga cggcgagagg atcctcccga ccgttgttca
     1441 ccacgatgtc cgctccgaac tgcttggcgg catcgagccg actgtcggcc aggtcgatgg
     1501 ccacgacgtg ggccgggctg aagaggcggg agccgatcat ggccgcgagg ccgatgggcc
     1561 cggccccgac gacggcgacc acgtcgccgg gtcgcacctt cccgttgagc accccgacct
     1621 cgtacccggt gggcaggatg tcggccagca tgaggatgtc ctcgtcggcc acgccgtcgg
     1681 gcacgacgta ggtcgacgtg tcggcgaacg ggacccgtac gtactcggcc tgggtgccgt
     1741 cgatcttgtg gccgaggatc caccctccgc caccgaggca ctggctgtaa ctgccctccc
     1801 ggcagtaacg gcacgccccg caggcggtga tgcaggacac gaggacgtgg tcgccgacag
     1861 cgacgttctt gactccggcg ccgacggact cgacggtccc cacagcttcg tggccgagga
     1921 tccgcccgtc ggtgacggcg gggacgtcgc ccttcaggat gtgcaggtcc gtgccgcaga
     1981 tcgtcacggc gtcgacgcgc acgacggcgt cggtgtcctc gatgatctcc ggcttgggta
     2041 cgtcctccca ggccttccga ccggggccgt ggtacacgac tgcacgcatg atgat
//
LOCUS       DASTFV010000375         2870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1364704, whole genome shotgun sequence.
ACCESSION   DASTFV010000375 DASTFV010000000
VERSION     DASTFV010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364704"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VFP02_06945"
     CDS             <1..299
                     /locus_tag="VFP02_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9542800.1"
                     /translation="RVEAQVERFAPGFRDRILARHVMNTRDVEARNANFIGGDINAGA
                     ADLRQFIRRPTLGLDPWRTPAKGVYLCSSSTPPGGGVHGMCGWHAAKSALKHEF"
     gene            470..>2870
                     /locus_tag="VFP02_06950"
     CDS             470..>2870
                     /locus_tag="VFP02_06950"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9542801.1"
                     /translation="MGLGLQALLARGTVRRGFGGLVAIAVVATLGLGAALTSIEAAER
                     TRSAYPDYLERADVADLVVNPVLANPRTEELIETTPGVRRVVSDSLLNAGPMPYPGDR
                     VGNFLQTRMSQDGRYLDQDRPVVRDGRMIGRSGQEAFLDVEGAEVLGVEVGDEMTLGF
                     FGVDPEDPAGDSLANPLGRSRVRVVGIGTFADEPLPQVGFPRTRILVTPDVAAEFDCL
                     QQTPDPTDEHTADELIVELVGLDCAMSYRYYSIDVEGGRDRAAAVSAELGRRFRDENA
                     HLPAAMRQAEIGYFLIPSFTADDARSVEQSLSPIITALRAFGVTAALATVVGVLVLVL
                     RHLRRREREVAVWRSLGLGAAGRSAGIGIGPVAAAALGALGGAVAALAASPVGPVASA
                     RTIVPHPGHSLTAVTLGASVAALFLLVVPTAVLAWRTAVRARAGVRARRRPSWEASLP
                     GSPMAALGLRVAFRGRDAMPAILGAASAVTVVTATLVFATGLDQLVDTPTRFGWPFDL
                     AVLGNAGYGPVDREAIAADLDRPGVDGWGLATLTGNLTIDGETAPILAGREGFDALVA
                     GTSVVDGHVPTGADEIGVGARTADDLKVGVGDEVTIGSPYGEHQVQVSGIVVLPTVGP
                     LESDSTSVGTGALLPAPLLEATYDGAEEATGMTAVELADVSAALVVVDLAPGVDPQAF
                     VDDLGERILGWDGSGAFLRVYAEPLRPAAIIDVAAMRGVPALLAGVFGLAMIATVVAG
                     LISGTRARRHEIAVLRALGATPRQRGLSIRVHAVTTMVVGLVVGLPLGVLLGRLAFRY
                     FA"
BASE COUNT          332 a         1052 c         1056 g          430 t
ORIGIN      
        1 atcgcgtcga ggcccaggtc gaacgcttcg ccccggggtt ccgcgaccgg atcctggccc
       61 gccacgtcat gaacacccgc gatgtcgagg cccgcaacgc caacttcatc ggcggggaca
      121 tcaacgccgg ggccgcggac ctccgccagt tcatccgccg ccccaccctc ggcctcgacc
      181 cctggcgcac gccggcgaaa ggcgtctacc tctgctcgtc gtcgaccccg ccgggcggtg
      241 gcgtccacgg catgtgcggc tggcacgcgg cgaagagcgc cctgaaacac gagttctgac
      301 cgttacctct gagtggcttc gctgcgctca ccgactccgg cgccgccgat cccggtggtc
      361 ggagatgctg ctccgctccg gtgcgcagcc gggggcaggg tggtggcacc ccccgccgat
      421 gtgggccagc gccctggctg gtccgatgac tccttcgtag ggttcggctg tggggttggg
      481 gctgcaggcg ctgctggcgc ggggcacggt tcgtcggggt ttcggtggcc tggtcgcaat
      541 cgccgtggtg gccacgctcg gcctcggtgc cgccctcacc agcatcgagg ccgccgagcg
      601 cacccgatcg gcctatcccg actacctcga gcgggccgat gtcgccgacc tcgtcgtcaa
      661 ccccgtgctg gccaatcctc ggaccgagga gctgatcgag accacgccgg gcgtccggcg
      721 ggtggtgagc gactcgctgc tcaacgccgg gccgatgccc tatcccggcg accgggtggg
      781 caacttcctg cagacccgga tgagccagga cgggcggtac ctcgaccagg accgcccggt
      841 ggtgcgcgac gggaggatga tcgggcgctc gggccaggag gccttcctcg acgtcgaagg
      901 cgccgaggtg ctgggcgtgg aggtcggcga cgagatgacc ctcggcttct tcggggtcga
      961 ccccgaagac ccggcggggg attcgctcgc caaccccctc ggacggtccc gggtgcgcgt
     1021 cgtggggatc ggcaccttcg ccgatgagcc gctgccccag gtggggttcc cgaggacccg
     1081 gatcctggtc acccccgacg tggcggcgga gttcgactgc ctccagcaga cccccgaccc
     1141 aaccgacgag cacacggccg acgagctgat cgtcgagctc gtcggcctcg actgcgcgat
     1201 gagctaccgc tactactcga tcgacgtgga gggcggccgc gaccgggccg cggcggtgtc
     1261 cgccgagctg gggcggcggt tccgtgacga gaacgcacac ctgccggcgg ccatgcgaca
     1321 ggcggagatc ggctacttcc tgatcccgtc cttcacggcc gacgacgccc ggagcgtcga
     1381 gcagtcgttg tcgccgatca tcaccgcgct gcgggccttc ggggtcaccg ccgccctggc
     1441 cacggttgtc ggtgtgctcg tcctggtgct gcgccacctc cgccgccggg agcgtgaggt
     1501 ggccgtctgg cgcagcctcg gactcggcgc cgccggccgg tccgccggga tcgggatcgg
     1561 tccggtggcc gcggccgccc tgggcgcact gggcggtgcg gtggcggcgc tggccgcatc
     1621 ccccgtcggg cccgtggcca gtgcccgcac gatcgttccc catcccggac actcgttgac
     1681 ggccgtgacc ctgggggcga gtgtcgccgc cctcttcctg ctggtggtcc cgacggccgt
     1741 gctggcctgg cggaccgccg tccgggcccg cgccggtgtc cgggcccggc ggcgcccgtc
     1801 ctgggaggcg agcctcccgg gctcaccgat ggcggcgctc ggcctccggg tcgcgttccg
     1861 gggccgggac gcgatgccgg caatcctcgg tgccgcgtcg gcggtcaccg tcgtgacggc
     1921 cacgctggtg ttcgccaccg gactggatca gctggtcgac accccgacgc gcttcgggtg
     1981 gccgttcgac ctcgccgtgc tgggcaacgc cgggtacggc ccggtcgacc gagaggccat
     2041 cgcggccgat ctcgaccgac ccggggtgga cggttggggc ctggccacgc tgaccgggaa
     2101 cctcacgatc gacggcgaga ccgcgccgat cctggccggg cgggagggct tcgacgcgct
     2161 cgtggccggg acctcggtcg tggacgggca cgtgcccacc ggcgccgacg agatcggcgt
     2221 cggcgcgcgg accgccgacg acctcaaggt cggcgtgggc gatgaggtga cgatcggcag
     2281 cccctacggc gaacaccagg tccaggtcag cggcatcgtg gtcctcccga cggtcgggcc
     2341 gctcgaatcg gacagcacct cggtgggcac cggcgccctg ctgccggcgc cgctgctgga
     2401 ggcgacctat gacggggccg aggaggcgac cggcatgacc gcggtcgagc tggcggacgt
     2461 ctcggcggcc ctggtggtcg ttgacctggc ccccggcgtc gatccgcagg ccttcgtgga
     2521 cgacctcggt gagcggatct tggggtggga cggcagcggg gcgttccttc gcgtctacgc
     2581 cgagcccctc cgcccggccg ccatcatcga tgtggccgcc atgcgaggcg tgcccgccct
     2641 gctggccggt gtcttcggcc tggccatgat cgccaccgtg gtcgccgggc tgatctcggg
     2701 cacgcgggca cggcgccacg agatcgcggt cctgcgcgcc ctcggggcca cccctcgcca
     2761 acgtggtctc tcgatccggg tccacgccgt gaccacgatg gtcgtcggcc tggtcgtggg
     2821 cctgccgctc ggcgtcctgc tcgggcgcct ggcgttccgg tacttcgccg
//
LOCUS       DASTFV010000376         3814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1372980, whole genome shotgun sequence.
ACCESSION   DASTFV010000376 DASTFV010000000
VERSION     DASTFV010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372980"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFP02_06955"
     CDS             complement(<1..347)
                     /locus_tag="VFP02_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542802.1"
                     /translation="MSLPSALDFAGRRVVVTGASSGIGAATAELFGGLGADVVGLDVA
                     DGSLDLREPAAIDAAVAALQGRPVWALVNCAGVPQTAGGRIVLTVNFLGTRYLTEALL
                     PHIVEGGAVASVAS"
     gene            complement(344..1519)
                     /locus_tag="VFP02_06960"
     CDS             complement(344..1519)
                     /locus_tag="VFP02_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9542803.1"
                     /translation="MDFAVTEEQADLRAELRQYFQDLLTPERQAKLWADEMGGPVWKE
                     AVKQIAADGWLGIGWPKEYGGQDRSPIEQLIFFDEAQRAEAPVPMLSINTVGPTIMRF
                     GTQDQKDRFLPGILRGELNFAIGYTEPGSGTDLASLKTRAVRDGDEYVVNGQKIFTSL
                     AAAADYVWLAARTDPDAPRHKGISIFLMPTDTPGFSVVPLNIMGGADTTATFYEDVRI
                     PVTNRVGGENEGWGLITNQLNHERVAICNAGLIWRYLEEVTDWAKATKTADGDRVIDE
                     QWVKLGLARVRASAEVLQLMNWKVAWALTVGDLSPADASATKVFGTHKYIESLGILME
                     IMGPISFLRAGSPDALLRGRMERSYQGILVLTFGGGTNEIQRDIIAMLGLGLPRAPR"
     gene            1566..2528
                     /locus_tag="VFP02_06965"
     CDS             1566..2528
                     /locus_tag="VFP02_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542804.1"
                     /translation="MRRIAVLAGLALVVVGCSGDDDVSVDDTTTTVATTTTVRAATPD
                     ELAAAVCAAQRIRGRVRVADPEVNELSGLVSLDSGLWANNDSGDTARVFRLDEQGETL
                     AVVNLEGITAFDWEDLSGAGPSAGELFAGDIGDNAAAREEIVVHRFAVPDPAPTGVVT
                     IPAADVQTITLHYPNGPRDAETLLVDPVTRDIVIVHKRFGGDSEVYQATEADWSNGDA
                     TLERVGAVAVGDSALDATTGGDVGFDGQVVALRTYAGLLVFPRQAEQSLAQALVDNES
                     CDAPTAIEVQGEAVAFTPDGYTTISEGNRPRINRFAVTPPTPGA"
     gene            2539..2961
                     /locus_tag="VFP02_06970"
     CDS             2539..2961
                     /locus_tag="VFP02_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542805.1"
                     /translation="MADRSDLETFEFAFNPRFSRLLRLGGITPETTLVTVTDDEYRIR
                     FGGWKLNTTIANCTGTCLTEGYSWFKAIGARGSFKDKGVTFGTTTDRGVCVLFVEPVP
                     ALVPGTLMNHPGATVTVADCEGLIAALERRGVPRNPQP"
     gene            complement(2980..>3814)
                     /locus_tag="VFP02_06975"
     CDS             complement(2980..>3814)
                     /locus_tag="VFP02_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET9542806.1"
                     /translation="EWDVLSALHQHDTGVPVPEPVALCEDETVTGAPFYVMGFADGTI
                     LRTVEDANRLEPAMLRAAADSLLDTQIALHAVDVEAIGLGDLGPPTGYVERQLERWRR
                     QYDDGRVRRIHLLEELHQRLARTVPPAIGKPGLIHGDYRFDNVVIGATGEITAVLDWE
                     LCTLGDPAADACWSLQYWADPDDEDTFLTSSPTTAVTLPRRLEMLGRYSALSGRPLDG
                     WPWFEIFGWWKMGCIVEGVHARRSKGQSAGAPSGPLETIAARADRFFQIAEQKARALG
                     L"
BASE COUNT          552 a         1338 c         1374 g          550 t
ORIGIN      
        1 gaggcgacgc tggcgaccgc gcccccctcc acgatgtgcg gcagtagcgc ctcggtcagg
       61 tagcgggtcc cgaggaagtt gaccgtcagg acgatccgcc cgcccgctgt ctgcggcacc
      121 cccgcgcagt tgaccaatgc ccagaccggc ctgccctgga gggcggcgac cgcggcgtcg
      181 atggcggcgg gttcccgcag atcgagcgag ccgtcggcca cgtcgagccc gaccacgtcg
      241 gccccgaggc cgccgaagag ctcggccgtg gcggcgccga tcccggacga agcgccggtg
      301 acgacgaccc gccggcccgc gaagtccagc gcgctgggca ggctcaccgc ggcgcgcggg
      361 gcaggccgag gccgagcatg gcgatgatgt cccgctggat ctcgttggtc ccgccgccga
      421 aggtcaggac caggatcccc tggtagctgc gctccatgcg cccgcggagc agggcgtcgg
      481 gcgagcccgc tcgcaggaag gagatggggc ccatgatctc catgaggatc cccaggctct
      541 cgatgtactt gtgggtgccg aagaccttgg tggcggacgc gtcggccggg ctgaggtcac
      601 cgaccgtcag cgcccaggcc accttccagt tcatgagctg gagcacctcg gcggaggccc
      661 gcacccgggc cagccccagc ttgacccact gttcgtcgat gacccggtca ccatcggccg
      721 tcttggtggc cttggcccag tcggtgacct cctccaggta gcgccagatg aggccggcgt
      781 tgcagatggc cactcgctcg tggttcagct ggttggtgat gaggccccag ccctcgttct
      841 cgccgcccac ccgattggtc acggggatgc ggacgtcctc gtagaaggtc gccgtcgtgt
      901 cggccccgcc catgatgttc aggggcacga ccgagaaacc gggggtgtcg gtgggcatca
      961 ggaagatgga gatgcccttg tgccgggggg cgtcggggtc ggttcgggcg gccagccaga
     1021 cgtagtcggc ggcggcggcg agggaggtga agatcttctg accgttcacg acgtactcgt
     1081 cgccgtcccg caccgctcgg gtcttgagcg aggccaggtc ggtgcccgag ccgggctcgg
     1141 tgtagccgat ggcgaagttg agctcacccc ggaggattcc gggcaggaag cggtccttct
     1201 ggtcctgcgt gccgaagcgc atgatcgtgg ggccgacggt gttgatggac agcatcggca
     1261 cgggtgcctc ggcccgctgg gcctcgtcga agaagatgag ctgctcgatc ggcgagcggt
     1321 cctggccgcc gtactccttc ggccagccga tgcccagcca cccgtcggcg gcgatctgct
     1381 tgaccgcctc cttccacacg ggcccgccca tctcgtcggc ccagagcttg gcctgcctct
     1441 ccggggtgag caggtcctgg aagtactggc gcagctcggc ccgcaggtcg gcttgctctt
     1501 cggtcacggc gaagtccacg cccgagtatc gcaggcgtca ggatgggcgg gtgaagggcc
     1561 gagggatgag acggatcgcg gtcctggccg ggctcgcact ggtcgtggtc gggtgctccg
     1621 gtgacgacga cgtctcggtc gacgacacga ccaccacggt cgccaccacg accaccgtgc
     1681 gcgcggcgac gccggacgag ttggccgcgg cggtgtgcgc ggcccagcgg atccggggtc
     1741 gggtgcgggt cgccgacccc gaggtgaacg agctgtccgg gctggtcagc ctcgactccg
     1801 gcctgtgggc caacaacgac agcggcgaca cggcccgggt gttccgcctc gacgagcagg
     1861 gcgagacgct ggccgtggtc aacctcgagg gcatcaccgc cttcgactgg gaggacctca
     1921 gcggggcggg accgtcggcg ggtgagctct tcgccggcga catcggggac aacgccgcgg
     1981 cccgggagga gatcgtggtc caccgcttcg ccgtgcccga cccagcgccg accggcgtcg
     2041 tgacgatccc cgcggccgac gtccagacga tcaccctgca ctacccgaac gggccccggg
     2101 acgccgagac cctgctggtg gatcccgtca cccgtgacat cgtcatcgtg cacaagcggt
     2161 tcggcggtga ctccgaggtg taccaggcga ccgaggccga ctggtccaac ggcgatgcca
     2221 ccctcgagcg ggtgggcgcc gtcgccgtgg gcgactccgc cttggacgcg accacgggcg
     2281 gtgacgtggg cttcgacggc caggtggtgg cgctccggac ctacgccggc ctgctcgtgt
     2341 tcccccgtca ggccgagcag tctctcgccc aggccctggt cgacaacgag tcctgtgatg
     2401 cgccgaccgc catcgaggtc cagggcgagg ccgtggcctt cacgcccgac ggctacacga
     2461 cgatcagcga gggcaaccga ccccggatca accgcttcgc cgtcacgcca ccgacgccgg
     2521 gcgcataggt tcggggcgat ggcggaccgg tcggacctcg agaccttcga gttcgccttc
     2581 aacccccgct tctcgcgcct cctgcgcctc ggcggcatca cgccggagac gaccctggtc
     2641 accgtcaccg acgacgagta ccggatccgg ttcggtggat ggaagctcaa caccaccatc
     2701 gccaactgca ccgggacctg cctgacggag ggctactcgt ggttcaaggc catcggggcc
     2761 cgcggctcgt tcaaggacaa gggcgtcacc ttcggcacga cgaccgaccg aggcgtctgc
     2821 gtcctcttcg tggagccggt gcccgcgctg gtccccggga ccctgatgaa ccatcccggc
     2881 gccacggtga cggtcgccga ctgcgagggc ctcatcgccg ccctggaacg ccgcggcgtc
     2941 ccccgcaacc cccagccctg accctccaac cagaacgggt cagaggccga gggcgcgggc
     3001 cttctgctcg gcgatctgga agaagcggtc ggcgcgggcg gcgatcgtct ccagcgggcc
     3061 ggacggggcg ccggcggact ggcccttgct gcgacgggcg tggacgccct ccacgatgca
     3121 gcccatcttc caccagccga agatctcgaa ccatggccag ccgtccagcg ggcgcccgct
     3181 gagggcggag tagcgcccca gcatctccag gcggcggggg agggtcaccg cggtcgtcgg
     3241 tgaggaggtc aggaaggtgt cctcgtcatc ggggtcggcc cagtactgga gcgaccagca
     3301 ggcatcggcc gcggggtcac ccagggtgca gagctcccag tcgaggacgg cggtgatctc
     3361 ccccgtcgca ccgatcacga cgttgtcgaa gcggtagtcg ccgtggatga gccccggctt
     3421 cccgatcgcg ggcgggacgg tgcgggccag ccgctggtgc agctcctcga gcaggtggat
     3481 ccgccgcacc cgaccgtcgt cgtactggcg gcgccagcgc tccagctgcc gctcgacgta
     3541 gccggtcggc gggccgaggt cacccaggcc gatcgcctcg acgtccacgg cgtggagggc
     3601 gatctgggtg tccaggagcg agtcggctgc cgcccgcaac atggccggct cgagccggtt
     3661 ggcgtcctcg accgtgcgca ggatcgtgcc gtcggcgaag cccatcacgt agaacggcgc
     3721 gccggtcacg gtctcgtcct cgcacagggc cacgggctcc gggacgggaa cgccggtgtc
     3781 gtgctggtgg agggcggaca gcacgtccca ctcc
//
LOCUS       DASTFV010000377         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1374813, whole genome shotgun sequence.
ACCESSION   DASTFV010000377 DASTFV010000000
VERSION     DASTFV010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374813"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1878)
                     /locus_tag="VFP02_06980"
     CDS             complement(<1..1878)
                     /locus_tag="VFP02_06980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET9542807.1"
                     /translation="MPSDTTTRDDADAADERPSIWGRYRRRRFRRRSIFWRWRRLLFL
                     AMLVVVASLAGVGLVLSNIELPPQSQSLAESSFICTREVTGRCGPDNAAAQLNGEENR
                     VIVPYRRIPEVLIDAVIAAEDQNFWEHNGVDPAGIARAGWAQVSGNSVSEQGGSTITQ
                     QYVKTVYLTNERTIARKIREAIYAVELERELSKEEILERYLNTVYFGRGAYGVQAASQ
                     AYFLEDVEDLSLTDAAYLAGLIRSPETADADRQPREAYRRRNTTLINMKELGYITQEE
                     YDDAIAVRWVVNPYGVCPDECPPGMVLPRTQAQLFSHVRGHDIGSEYFYETVRQQLVS
                     LFGEETVYGGGLRVYTTLDQNWQRQAYETVVNNYPSGAPTSSIVAIGADGRVRAMMGG
                     RDFNISELNLATGAKGGGSGRQPGSSFKPFALAEAFAQGYSAEATLPAPSSVTLDIPG
                     TADWHVSGGASASGSHTLLSGIQASSNVVFAQLMLELGPENVIEMAHTLGIKSDLPAV
                     PSIVLGAGEVSVLDMASAYSSFRDHGLHHEPVMIERVEDARGNVIYRADESPQQVISP
                     EVADTVTTALRGVVSGGTGVQAQLSGWEVAGKTGTTQSNKDAWFVGYTCNVTTAVWVG
                     NV"
     gene            complement(1980..2363)
                     /locus_tag="VFP02_06985"
     CDS             complement(1980..2363)
                     /locus_tag="VFP02_06985"
                     /inference="COORDINATES: protein motif:HMM:NF036456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HET9542808.1"
                     /translation="MSFRVEAIRGAEHGGTVDYRLARAGVLAEYRKGRLARHEVCDAH
                     PELLRAARDYIEESTQLDCPVCEEEKLKLVSYVFGSYLGPGGRCVRDRAELAKVTRRK
                     GTYRCYVVEVCPGCGWNHLSRVFEV"
     gene            2526..>2822
                     /locus_tag="VFP02_06990"
     CDS             2526..>2822
                     /locus_tag="VFP02_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9542809.1"
                     /translation="MAAVEAGDRDGWLALFSDDAVVEDPIGPSPLNPDGAGRRGQAAI
                     AAFWDEIISIGQVRFALRESYACGDECANVGRITTTLPDGNRSVVEGVYTYRVDG"
BASE COUNT          419 a          975 c         1002 g          426 t
ORIGIN      
        1 gacgttgccg acccacaccg cggtggtgac gttgcaggtg taccccacga accaggcgtc
       61 cttgttgctc tgcgtggtcc cggtcttgcc ggccacctcc cagcccgaca gctgcgcctg
      121 cacgccggtg ccgccggaga cgacgccccg cagcgcggtg gtgacggtgt cggccacctc
      181 gggggagatg acctgctggg gcgactcgtc ggcgcggtag atcacgttgc cgcgggcgtc
      241 ctcgacccgc tcgatcatca ccggctcgtg gtgcagcccg tggtcccgga agctgctgta
      301 ggcgctggcc atgtccagca ccgacacctc accggcgccg agcacgatcg acgggaccgc
      361 gggcagatcg gacttgatgc cgagggtgtg ggccatctcg atcacgttct ccggaccgag
      421 ctcgagcatg agctgggcga agaccacgtt ggaggaggcc tggatgccgc tcagcaacgt
      481 gtggctccct gacgcggagg cgccgccact gacgtgccag tccgccgtgc ccgggatgtc
      541 cagggtcacg ctgctcggcg cgggcagggt ggcttcggcc gagtagccct gggcgaaggc
      601 ctcggccagg gcgaagggct tgaacgacga gccgggctgg cggccggagc cgccgccctt
      661 ggcccccgtg gccaggttca gctcggagat gttgaagtcc cggccgccca tcatcgcccg
      721 cacccggccg tcggccccga tggccacgat cgacgaggtc ggcgccccgg acgggtagtt
      781 gttgacgacg gtctcatagg cctggcgttg ccagttctgg tccagcgtcg tgtacacgcg
      841 gaggccgccg ccgtagacgg tctcctcgcc gaagaggctc accagctgct ggcgcacggt
      901 ctcgtagaag tactccgagc cgatgtcgtg gccccgcacg tgggagaaga gctgggcctg
      961 ggtccggggc aggaccatgc ccggcgggca ctcgtccggg cagacgccgt agggattgac
     1021 gacccacctg acggcgatgg cgtcgtcgta ctcctcctgg gtgatgtagc ccagctcctt
     1081 catgttgatc agcgtggtgt tccgacgccg gtaggcctcg cggggctgcc ggtcggcatc
     1141 cgccgtctcc ggcgaccgga tcaggccggc aaggtacgcg gcgtcggtca gggacaggtc
     1201 ctcgacgtcc tcgaggaagt acgcctgcga ggccgcctgc acgccgtagg cgccgcggcc
     1261 gaagtagacc gtgttcaggt agcgctcgag gatctcctcc ttcgagagct cgcgctccag
     1321 ctcgacggcg tagatggcct cgcggatctt gcgggcgatc gtgcgctcgt tggtcaggta
     1381 gaccgtcttc acgtactgct gggtgatggt cgacccaccc tgctcggaca cgctgttccc
     1441 cgacacctgg gcccatcccg cgcgggcgat accggcgggg tccacgccgt tgtgctccca
     1501 gaagttctgg tcctcggcgg cgatgacggc gtcgatcagg acctcgggga tgcgccggta
     1561 ggggacgatg acccggttct cctcgccgtt gagctgggcc gcggcgttgt cgggcccgca
     1621 gcgcccggtc acctctcggg tgcagatgaa ggaggactcg gccaggctct ggctctgggg
     1681 cggcagctcg atgttggaga gcaccaggcc gaccccggcc aagctggcga cgaccacgag
     1741 catggccagg aacagcagcc ggcgccagcg ccagaagatg ctgcggcggc ggaagcggcg
     1801 gcggcggtag cggccccaga tggagggacg ctcgtcggcc gcatcggcgt cgtcgcgggt
     1861 ggtggtgtcc gaaggcatgc aggaagaaga ggtcggggaa tctcgggccg ggaaatcgcg
     1921 ccggcccccg aggttaccgt tcgattacgg gatgtttccg tcacgggacg cgacgggggt
     1981 cacacctcga acaccctcga caagtggttc cagccacagc cgggacacac ctcgaccacg
     2041 taacagcggt aggtgccctt gcgccgggtg accttggcca gctccgcccg gtcccgcacg
     2101 caacgcccgc cggggcccag gtaggacccg aagacatagg agaccagctt gagcttctcc
     2161 tcctcacaca ccgggcagtc cagctgggtg gactcctcga tgtagtcccg cgccgcccgc
     2221 agcagctcgg ggtgggcgtc gcagacctcg tggcgggcca gacggccctt gcggtactcg
     2281 gccagcacgc ccgcgcgcgc cagtcggtag tcgaccgtgc cgccgtgctc ggcgccccgt
     2341 atcgcctcca cccggaagct catggggtcg gaggctaggt cgtcatcggg gaccgaggga
     2401 gccggcgcgc tagaaacccc cttgtgagcg aggctgcgga accgcgtgga gcagcacttc
     2461 cgaccgccga gatcggcgga gccggcggag cagagaaccc ggctcgactg gcctcccaac
     2521 ggtcgatggc ggcggtcgag gccggcgacc gcgacgggtg gctggccctg ttcagcgacg
     2581 acgccgtggt cgaagacccc atcggtccct ccccgctcaa ccccgacggc gccggccggc
     2641 gaggccaggc cgccatcgcc gccttctggg acgagatcat ctccatcggc caggtccgct
     2701 tcgctctccg cgagtcctac gcctgcggcg acgaatgcgc caacgtcggc cgcatcacca
     2761 ccacgctgcc cgacggcaac cgctcggtcg tcgaaggcgt ctacacctac cgggtcgacg
     2821 gc
//
LOCUS       DASTFV010000378         4043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1375781, whole genome shotgun sequence.
ACCESSION   DASTFV010000378 DASTFV010000000
VERSION     DASTFV010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375781"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..2662)
                     /gene="ppdK"
                     /locus_tag="VFP02_06995"
     CDS             complement(65..2662)
                     /gene="ppdK"
                     /locus_tag="VFP02_06995"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HET9542810.1"
                     /translation="MTWIYGFAHPHATPPRDLKDLLGGKGANLAEMTSVLGLPVPPGF
                     TITTEACVAYLAEGWPDGLDAEIAEHVAALEEAMGRQFGDPDDPLLLSVRSGAKFSMP
                     GMMDTILNLGLNDESAAGLAEVTGHRWFALDSYRRLIQMYGTTVAGVDGEVFDHVDGL
                     DEERLATEVEGFGRAYEGAVGSPFPQEPERQLREAVEAVFRSWNTPRADAYRRREGIP
                     DDLGTAVNVQVMVFGNRDDRSGTGVGFTRDPATGAPGLYGDFLVNAQGEDVVAGIRNT
                     LPLTELGEHFPDLAGELQEIFDLLEQHYRDMMDIEFTIEQDKLWILQTRVGKRTGAAA
                     VRLAVAMNQDEHIALSPAEAVDRVLPEHIDQLLHPQLADFPPEDLLTTGLGASPGAGV
                     GRVYFTADACVAAVEAGERAVLVRHETSPEDIHGMAAAEGVLTARGGLVSHAAVVARQ
                     WGKPAVVGAQEIEVRESSMEVGGVTLEEGDYLSIDGTTGDVVRGEVATTEGKLPDELT
                     TVLTWADEIRAERLGVRANADTGADAARARAWGAEGIGLCRTEHQFLGDRLPLVRRMI
                     LAETDEDEVAALEELGAQQRVDFEGLLEAMDGLPVTVRLLDPPLHEFLPDLDELIAGE
                     ARDELDAEEVELLAAGRKWREQNPMLGVRGVRLAVLREGLYRTQVRALLDAAEARTKA
                     GGTPIVEVMIPLVVVREELALARSWVEEELAARPEGATGTVRIGTMIETPRACVRAHE
                     IAEVADFFSFGTNDLTQLTFGFSRDDVEGPLMTAYLERGLLAADPFSTVDQGGVADLI
                     RLGVEGGRRTHPGLKVGVCGEHGGDPASIAVFLSLGVDYVSCSPFRVPIARLAAARAV
                     LDEATTPAH"
     gene            complement(2686..3501)
                     /locus_tag="VFP02_07000"
     CDS             complement(2686..3501)
                     /locus_tag="VFP02_07000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9542811.1"
                     /translation="MGDEAEYQRLNRLWWDERAPVHADSDFYDLDGFVAGRDTLQPFE
                     PEVVGEVAGLDLVHLQCHMGMDTLSWARRGARVTGLDFSAPAIDTARSLADRIGVEDA
                     RFVVADVYDAVEVLGTDYDLAYQSLGSLNWHPDIDRWAAVLAALIRPGGRYFLLEAHP
                     LGWILDDDGERVENGYFFDPRGNRWIDEGGTYADLSAKTVNNETLEYTHAVSEVITAL
                     LGAGFVLEHFAEHPYTVHAMWPWLEERGHRQWFAPEGRPALPMMYSLMARRSD"
     gene            complement(3501..>4043)
                     /locus_tag="VFP02_07005"
     CDS             complement(3501..>4043)
                     /locus_tag="VFP02_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HET9542812.1"
                     /translation="RFPWGFDELEGIANRTDFDLKAHAEHSGEALDFFDPATETRYVP
                     HVIEPAAGATRTLMAFLLSAYDEEEVKGETRVVLRLHHRLAPYKVAVLPLSRKEELTG
                     PAREVFALLAPEFMCDYDETQSIGRRYRRQDELGTPYAVTFDFDSLEDNAVTVRERDS
                     MEQERIPVDDLVAYLRERMA"
BASE COUNT          569 a         1459 c         1417 g          598 t
ORIGIN      
        1 cccaggcggg caccgcgaag tcggcgtagt tggcggcctc ctgggcggcg acgagcatct
       61 cggatcagtg tgccggggtc gtcgcctcgt cgagcaccgc ccgggccgcc gccaatcggg
      121 cgatcggtac ccggaagggc gaacaggaca cgtagtcgac cccgaggctc aggaacaccg
      181 ctatcgacgc cgggtcgcca ccgtgctcgc cgcaaacccc gaccttcagg ccggggtgcg
      241 tgcgtcgacc gccctcgacc ccgaggcgga tcaggtcggc gacgcccccc tggtcgacgg
      301 tgctgaacgg gtcggcggcc agcaggcccc gctcgaggta ggcggtcatc agcgggccct
      361 ccacgtcgtc ccgactgaag ccgaacgtca gctgggtcag gtcgttggtg ccgaacgaga
      421 agaagtccgc cacctcggcg atctcgtggg cccgcacgca cgcccggggc gtctcgatca
      481 tggtgccgat ccgcaccgtc ccggtggccc cctcgggtcg ggcggccagc tcctcctcga
      541 cccacgagcg ggccagggcc agttcctcgc ggacgacgac aagggggatc atgacctcga
      601 cgatcggcgt tccccccgcc ttcgtccggg cctcggccgc gtcgagcagg gcccgcacct
      661 gcgtgcggta gaggccctcc cggagcaccg ccaggcgcac gccccggacg cccagcatcg
      721 ggttctgctc ccgccacttc cgcccggcgg ccagcagctc gacctcctcc gcgtcgagct
      781 cgtcccgtgc ctctcctgcg atgagctcgt ccaggtcagg caggaactcg tgcagcggcg
      841 ggtcgaggag gcgcacggtc acgggtagcc cgtccatggc ctcgagcaac ccctcgaagt
      901 ccacccgctg ctgggctccg agctcctcca gggcggcgac ctcgtcctcg tcggtctcgg
      961 ccaggatcat gcggcggacc aaggggagac ggtcaccgag gaactggtgc tcggtccggc
     1021 agagcccaat cccctcagcg ccccacgccc gggcccgggc cgcgtcggcg ccggtgtcgg
     1081 cgttggcccg caccccgagc cgctccgccc gtatctcgtc cgcccacgtc aggaccgtcg
     1141 tcagctcgtc cggcagcttc ccctcggtcg tcgccacctc gccccgcacg acgtcgccgg
     1201 tggtgccgtc gatcgacagg tagtcccctt cctccagggt cacaccgccg acctccatcg
     1261 acgactcgcg gacctcgatc tcctgggctc cgaccacggc gggcttgccc cactgccggg
     1321 cgacgacggc ggcgtggctg acgaggcccc cgcgggcggt gagcacccct tcggccgccg
     1381 ccatgccgtg gatgtcctcc ggcgaggtct cgtggcggac gaggaccgcc cgctcccccg
     1441 cttcgacggc ggccacgcat gcgtcggccg tgaagtacac ccgtcccacg ccggcgccgg
     1501 ggctggcgcc caacccggtc gtcagcaggt cctccggggg gaagtcggcg agctgggggt
     1561 gcaggagctg gtcgatgtgt tcgggcagga cccgatcgac cgcctcggcc gggctcaggg
     1621 cgatgtgctc gtcctggttc atggccacgg cgaggcggac cgcggcggca ccggtgcgct
     1681 tcccgacccg ggtctggagg atccagagct tgtcctgctc gatggtgaac tcgatgtcca
     1741 tcatgtcccg gtagtgctgc tcgaggaggt cgaagatctc ctgcagctca ccggccagat
     1801 ccgggaagtg ctcgccgagc tcggtcagcg gcagggtgtt gcggatgccg gcgacgacgt
     1861 cctcaccctg ggcgttcacg aggaaatcgc cgtacagccc cggtgccccg gtggcggggt
     1921 cccgggtgaa gcccacaccg gtccccgagc ggtcatcgcg gttgccgaac accatgacct
     1981 ggacgttcac ggccgtcccg aggtcgtccg ggatcccctc ccggcgtcgg taggcgtcgg
     2041 cccggggcgt gttccacgag cggaacaccg cttccaccgc ctcgcgcaac tgccgttccg
     2101 gctcctgcgg gaacggtgag ccgacggcac cctcgtaggc ccggccgaag ccttccacct
     2161 cggtggccag ccgctcctcg tcgaggccgt cgacgtggtc gaacacctcg ccgtcgaccc
     2221 cggccaccgt cgttccgtac atctggatca ggcgccggta gctgtcgagg gcgaaccacc
     2281 ggtggccggt gacctcggcg aggccggcgg cgctctcgtc gttgagtccg aggttcagga
     2341 tcgtgtccat catccctggc atcgagaact tggcccccga gcgcacgctg agcagaagcg
     2401 gatcgtcggg gtcaccgaac tgacggccca tcgcctcctc cagtgcggcc acgtgctcgg
     2461 cgatctcggc gtccagtccg tccggccagc cctcggccag gtaggcgaca caggcctcgg
     2521 tggtgatggt gaagccgggc gggacgggga gccccagcac ggacgtcatt tcggccaggt
     2581 tggcgccctt gccgccgagc aggtccttca ggtcccgggg cggcgtcgcg tggggatgag
     2641 cgaagccgta gatccaggtc accgccggac cctacccagc gccgatcagt cggatctccg
     2701 ggccatcagc gagtacatca tcgggagggc gggccgtccc tccggggcga accactgccg
     2761 gtggccccgc tcctccagcc acggccacat ggcgtggacc gtgtaggggt gctcggcgaa
     2821 gtgctcgagg acgaagccgg cccccaggag cgcggtgatg acctcgctca ccgcgtgggt
     2881 gtactccagg gtctcgttgt tgaccgtctt cgccgagagg tcggcatagg tgcctccctc
     2941 gtcgatccag cggttcccgc gggggtcgaa gaagtagccg ttctcgaccc gctccccgtc
     3001 gtcgtcgagg atccaaccca gggggtgggc ctcgagcagg aagtagcgac cacccgggcg
     3061 gatcagcgcg gcgaggacgg cggcccaacg gtcgatgtcc ggatgccagt tgagcgaccc
     3121 caggctctgg taggcgaggt cgtagtcggt gccgaggacc tcgacggcgt cgtagacgtc
     3181 ggcgaccacg aaccgggcgt cctcgacacc gatccggtcg gccaggctcc gggccgtgtc
     3241 gatggccggc gccgagaagt cgaggccggt cacccgggcg ccccgccggg cccacgacag
     3301 ggtgtccatc cccatgtggc actgcaggtg gaccaagtcg agtccggcga cctcaccgac
     3361 gacctcgggc tcgaagggct gcagcgtgtc gcgaccggcg acgaagccat ccaggtcgta
     3421 gaagtcactg tcggcgtgga cgggggcgcg ctcgtcccac cacagccggt tgaggcgctg
     3481 gtactccgcc tcgtcaccca tcaggccatc cgctcccgca ggtacgcgac gaggtcgtcg
     3541 acggggatcc gctcctgctc catcgagtcg cgctcgcgga cggtgacggc gttgtcctcg
     3601 agcgagtcga agtcgaaggt cacggcgtac ggcgtgccca gctcgtcctg gcggcggtac
     3661 cgccggccga tggactgggt ctcgtcgtag tcacacatga actcgggggc gagcaacgcg
     3721 aacacctccc gggccgggcc ggtcagctcc tccttgcggg acaggggcag cacggcgacc
     3781 ttgtagggcg caagccgatg gtggaggcgg agcacgacgc gggtttcgcc cttgacctcc
     3841 tcctcgtcgt aggccgagag caggaaggcc atgagcgtcc gggtggcgcc tgccgcgggc
     3901 tcgatcacgt ggggcacgta gcgggtctcg gtggccgggt cgaagaagtc cagcgcctcg
     3961 cccgagtgct cggcgtgcgc cttgaggtcg aagtcggtgc ggttggcgat cccctcgagc
     4021 tcgtcgaacc cccaggggaa gcg
//
LOCUS       DASTFV010000379         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1377229, whole genome shotgun sequence.
ACCESSION   DASTFV010000379 DASTFV010000000
VERSION     DASTFV010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377229"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VFP02_07010"
     CDS             complement(<1..652)
                     /locus_tag="VFP02_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542813.1"
                     /translation="MTGGTSGTTELRRGAELLALTGLAITQPVLDVLGNSPETFVFRG
                     VDGAELVVFALLVALVPGLALWALGAATRVFGDRVRAGVHLATVAALVGVAVLVAVRL
                     ADLAKGPTALLIAVAAAVGGGVLYSRVAGVRSFLLYLAPLPLVAAALFLFSSPVSGLL
                     DGGDTEVVEDVGSDQPVVMLVLDEFPTATLLDDGGQIDVTQFPNFARLAEGSTWFRN"
     gene            complement(666..2285)
                     /locus_tag="VFP02_07015"
     CDS             complement(666..2285)
                     /locus_tag="VFP02_07015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9542814.1"
                     /translation="MASPDLTAPDLNGATAAVDLAKGVVEGATRHLAAAGDLDDNQVI
                     AYDLAHAAAVVETGRALLDYGGKGDEEGAITCAYIADGVADLTGKLYGREAAWGVEPG
                     ALDTTREFVAAHRDPAFLASLAEAPGPLHLDEEFELVRDTFRRFAEDKIRPVAEHVHR
                     TNGDVPEEIIQGIAELGGFGLSVPAEYGGFAEGGESDYLAMVVATEELSRGSLGCGGS
                     LITRPEILSRALVKGGTEEQKHEWLPKLASGEVMAAVAVTEPDFGSNVAGIKVTAVPT
                     DGGWLINGVKTWCTFGARADVLMLLARTDPDRSKTHRGLSMFVVPKPRGDGHGFELTQ
                     EPGDHGGAPGGGTMEGRPIDTIGYRGMHSYEIALDDWFVAAENQVGGEEGLGRGFYLQ
                     MEGFENGRLQTAARAVGIMQAAYEEARQYAKDRVVFDQPVAEYQLTQAKLGRMAFTIQ
                     AARQFSYVVARMMAKGDGALEASMVKAYVCKAAEWVTREAMQIHGGFGYAEEYPVSRY
                     FVDARVLSIFEGTDELLCMRVITRRLVERAS"
BASE COUNT          357 a          837 c          825 g          318 t
ORIGIN      
        1 agttgcggaa ccaggtcgac ccctccgcca gccgggcgaa gttggggaac tgggtgacgt
       61 cgatctgtcc cccgtcgtcg agcagcgtgg ccgtcgggaa ctcgtcgagg accagcatga
      121 ccaccggctg gtcgctgccc acgtcctcga cgacctcggt gtcacccccg tccagcagac
      181 cagagacggg cgacgagaag aggaacagcg cggcggccac gagagggagg ggcgcgaggt
      241 agagcaggaa cgagcggacc ccggcgaccc ggctgtagag gacgcccccg ccgacggcgg
      301 cggccacggc gatgaggagg gcggtcggcc ccttggccag gtcggccagg cggacggcca
      361 cgagcacggc cacgccgacg agggcggcga ccgtcgcgag gtgcaccccg gcgcggacgc
      421 gatccccgaa cacgcgggtg gcggcaccca gggcccagag ggccagaccg ggcacgaggg
      481 cgacgagcag ggcgaagacg accagttcgg ccccgtccac cccgcggaac acgaaggtct
      541 cggggctgtt gccgagcaca tcgagcacgg gctgggtgat ggccaacccg gtgagggcca
      601 gcaactccgc accccgccga agctcggtcg tcccgctcgt ccccccggtc aaggacgtga
      661 tgttgtcagc tcgcccgctc caccaagcgg cgggtgatca ccctcatgca gaggagctcg
      721 tcggtgccct cgaagatcga caacacccgg gcgtcgacga agtaccggct gacggggtac
      781 tcctccgcat agccgaaccc gccgtggatc tgcatggcct cacgggtcac ccactcggcg
      841 gccttgcaca cataggcctt gaccatcgac gcctcgagcg ccccgtcgcc cttggccatc
      901 atccgggcga cgacgtagct gaactggcgg gcggcctgga tggtgaaggc cattcgaccg
      961 agcttggcct gggtcagctg gtactcggcg acgggttgat cgaacaccac ccggtccttg
     1021 gcgtactgac gggcttcctc gtaggcggcc tgcatgatgc cgacggcgcg ggccgcggtc
     1081 tgcaggcgcc cgttctcgaa gccctccatc tgcaggtaga acccccggcc cagcccctcc
     1141 tccccgccga cctggttctc ggcggccacg aaccagtcgt cgagggcgat ctcataggag
     1201 tgcatgcccc ggtagccgat ggtgtcgatc ggccggccct ccatcgtccc gccgcccggc
     1261 gccccaccgt ggtcgcccgg ctcctgggtc agctcgaagc cgtggccgtc gcctctcggc
     1321 ttggggacga cgaacatgga cagcccccgg tgggtcttgg accggtcggg gtcggtgcgg
     1381 gccagcagca tgaggacgtc ggcccgggcg ccgaacgtgc accacgtctt gacgccgttg
     1441 atcagccagc cgccgtcggt ggggacagcg gtgaccttga tgccggcgac gttggagccg
     1501 aagtcgggct ccgtgacggc caccgcggcc atgacctcgc ccgacgccag tttgggcagc
     1561 cactcgtgct tctgctcctc ggtcccgccc ttgaccaggg cccgggagag gatctcgggg
     1621 cgggtgatga gcgacccgcc acagcccagg gagccgcggg acagctcctc ggtggccacg
     1681 accatggcca ggtagtccga ctcgccgccc tcggcgaagc cgccgtactc ggccggcacg
     1741 gacaggccga agccgcccag ctcggcgatg ccctggatga tctcctcggg gacgtcaccg
     1801 ttggtgcggt ggacgtgctc ggcgaccggg cggatcttgt cctcggcgaa ccgccggaac
     1861 gtgtccctga ccagctcgaa ctcctcgtcc aggtgcagcg ggccgggggc ttcggccagc
     1921 gaggccagga aggccgggtc ccggtgggcg gccacgaact cccgagtggt atccagtgct
     1981 cctggctcga ccccccacgc cgcctcccgc ccgtagagct tgcccgtcag atcggccacg
     2041 ccatcggcga tgtaggcgca ggtgatggcc ccctcctcgt cgcccttgcc gccatagtcc
     2101 aggagcgccc ggccggtctc gaccacggcc gccgcgtggg ccaggtcgta ggcgatcacc
     2161 tggttgtcgt cgaggtcgcc ggcggcggcg aggtggcggg tggcgccctc gaccacaccc
     2221 ttggcgaggt cgacggcagc agtcgcgccg ttcaggtctg gagcggtcag gtcaggcgag
     2281 gccatggccg cattatcggc ggcgcgcgac caccgtcacc aacggaggca gcacgac
//
LOCUS       DASTFV010000380         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1385035, whole genome shotgun sequence.
ACCESSION   DASTFV010000380 DASTFV010000000
VERSION     DASTFV010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385035"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFP02_07020"
     CDS             complement(<1..435)
                     /locus_tag="VFP02_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HET9542815.1"
                     /translation="MDPLLTGDLTAGDVGIVLLKVVVAFVFLLVAVLFMIWFERKLIS
                     DMQNRIGPDLAGPWGLFQTFADGVKFFFKEDLKPIRADLGVFRLAPYLSIIPAFLVFC
                     IVPIGGNFKGPDEGVVSIFGHDTLLQVADPPIGILLFLALSSI"
     gene            complement(449..>2628)
                     /locus_tag="VFP02_07025"
     CDS             complement(449..>2628)
                     /locus_tag="VFP02_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9542816.1"
                     /translation="VVDTHSEATKKAQDGVLEFLLINHPLDCPVCDKGGECPLQDQTV
                     AYGPGESRFVEEKRHFAKPIPVNDVVLLDRERCILCDRCTRFAKDVAGDPLISFIGRG
                     AATQVNTFPDHPFSSYFSGNTVQICPVGALTAGPFRFRARPWDLANAESTCTTCSVGC
                     RVAIQSSRDEVLRYSGVDVDPVNWGWLCDKGRFDFSYVNNPDRLGEPLVRTPGGSGLT
                     TARWSEALGRAAEALEAAPADRIGVIGGARLTNESAYAWAKLAKGVLGTDNVDAQLGD
                     GLPADLVLGLPRATIDEACAPGTTVVLLGPDIKEELGVLYLRLRHAAVEDGVRIIEIA
                     PATSGLTPYATHTELHAPGGAPAAVASVLDAGLLGDGPVVVLLGRPSLAEPAAHTAGA
                     ALALAEVGDRVRFLSTLRRGNVHGALDMGLAPGVLPGRVTLDGGREALADAWPMVPPS
                     AGLDTDAMLRAGADGGLDVLVLLGADPVQDAPDRDLARRALEAVGTVIAVDCFLTGSV
                     EAADVVLPAAGYAEVDGTTTNLEGRVSTVVQRVTSPGTARADWIIATDLAARLGADLG
                     VTSVEDLLAEIERVAPTHAGLGDRLVAAGGDGVLVEGSSVTAGAVTDGHLPAADSYSL
                     RLASGRTMYDEATLVQRASTIAKLARPATVRLNPHDFDRLGIETGTPVKVTSARGSIT
                     VSASADAGVPRASAVVPFNAPGGAGGDLISAGEAVTDVRVERL"
BASE COUNT          380 a          867 c         1053 g          328 t
ORIGIN      
        1 gatcgaggag agggccagga agagcaggat gccgatgggc gggtcggcca cctgcaggag
       61 ggtgtcgtgg ccgaagatgg agacgacgcc ctcgtcgggc cccttgaagt tgccgccgat
      121 gggcacgatg cagaagacca ggaaggccgg gatgatcgag aggtaggggg cgaggcggaa
      181 gacgccgagg tcggcccgga tcggcttgag gtcctccttg aagaagaact tcacgccgtc
      241 ggcgaaggtc tggaacaggc cccacggccc ggccaggtcg gggccgatcc ggttctgcat
      301 gtcgctgatg agcttgcgtt cgaaccagat catgaacagc acggccacga gcaggaagac
      361 gaaggcgacc acgaccttca acaggacgat gccgacgtcg cccgccgtga ggtcgccggt
      421 gaggaggggg tccatcagtg cgcccccatc acagtcgctc cacccggacg tcggtcacgg
      481 cctcgccggc gctgatcagg tcgccgccgg cgccgccggg agcgttgaac ggcaccacgg
      541 cgctggcgcg gggcaccccg gcgtcggccg aggccgacac cgtgatcgag ccccgggccg
      601 aggtcacctt gaccggggtc ccggtctcga tgccgagccg gtcgaagtcg tgggggttca
      661 ggcggaccgt ggccgggcga gccaacttgg cgatggtcga ggcccgctgg accagggtgg
      721 cctcgtcgta catggtccgg ccgctcgcca ggcgcaggga gtagctgtcc gccgccggca
      781 ggtgcccgtc ggtgaccgcg ccggcggtga ccgacgagcc ctcgaccagg acgccgtcac
      841 cgcccgccgc gaccaggcgg tcgcccaggc cggcgtgggt cggggccacc cgctcgatct
      901 cggccagcag gtcctcgacc gaggtcacgc ctaggtcggc cccgaggcgg gcggcgaggt
      961 cggtggcgat gatccagtcg gcccgggccg tgcccgggct ggtgacccgc tggaccacgg
     1021 tgctgacccg accctcgagg ttggtggtgg tgccgtcgac ctcggcgtag ccggcggcgg
     1081 gcaggacgac gtcggccgcc tcgaccgagc cggtgaggaa gcagtccacg gcgatcaccg
     1141 tgcccaccgc ctcgagtgcc cggcgagcca ggtcccggtc cggcgcgtcc tggaccggat
     1201 cggcgccgag gaggacgagg acgtccaggc caccgtcagc cccggcgcgg agcatcgcgt
     1261 ccgtgtccag gccggcgctc ggcggaacca tcggccaggc gtcggccagg gcctcacggc
     1321 cgccgtccag cgtgacccga ccgggcagga cgccaggggc caggcccatg tcgagggcgc
     1381 cgtggacgtt gccgcggcgg agcgtggaga ggaagcggac ccggtcgccg acctcggcca
     1441 gggccagggc ggcccccgcc gtgtgggccg cgggctcggc cagcgagggg cgaccgagga
     1501 ggacgaccac cgggccgtcg cccaggaggc cggcgtcgag caccgaggcc acggcggcgg
     1561 gggcgccgcc gggggcgtgg agctcggtgt gagtggcgta cggggtgagg cccgaggtgg
     1621 ccggggcgat ctcgatgatc cggaccccgt cctcgactgc ggcgtggcgc aggcggaggt
     1681 agaggacgcc gagctcctcc ttgatgtcgg ggccgaggag gaccacggtc gtgccggggg
     1741 cgcaggcctc gtcgatggtg gcccggggca gcccgaggac caggtcggcg ggcaggccgt
     1801 cgcccagctg ggcgtccacg ttgtcggtgc cgagcacgcc cttggccagc ttggcccagg
     1861 cgtaggccga ctcgttggtc aagcgggcgc caccgatgac gccgatccgg tcggcgggtg
     1921 cggcctcgag ggcctcggcg gcccggccga gggcctcgga ccagcgggcg gtggtgaggc
     1981 cactgccgcc cggcgtgcgg accagcggct cgcccaggcg gtcggggttg ttgacgtagg
     2041 agaagtcgaa gcggcccttg tcgcagagcc acccccagtt gaccgggtcg acgtcgaccc
     2101 cgctgtagcg caggacctcg tcccgggagg actggatggc cacccggcag ccgaccgagc
     2161 aggtcgtgca ggtggactcg gcgttggcca ggtcccaggg ccgggcccgg aagcggaagg
     2221 gcccggcggt gagggcgccc accgggcaga tctggaccgt gttgccgctg aagtaggagg
     2281 agaacgggtg gtcggggaag gtgttgacct gcgtcgccgc gccccggccg atgaaggaga
     2341 tcagggggtc gccggccacg tccttggcga agcgggtgca gcggtcacag aggatgcagc
     2401 gctcccggtc gaggaggacg acgtcgttga ccgggatggg cttggcgaag tgccgcttct
     2461 cctcgacgaa gcggctctcg ccggggccgt acgcgacggt ctggtcctgc agggggcact
     2521 cgccaccctt gtcacagacc gggcagtcca gcgggtggtt gatgagcagg aactcgagga
     2581 cgccgtcctg ggccttcttg gtggcttcgc tgtgggtgtc caccacca
//
LOCUS       DASTFV010000381         2353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1388463, whole genome shotgun sequence.
ACCESSION   DASTFV010000381 DASTFV010000000
VERSION     DASTFV010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388463"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VFP02_07030"
     CDS             <1..603
                     /locus_tag="VFP02_07030"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542817.1"
                     /translation="AEQAASGVPSGIMDQLASAAGVENHALLVDCRSLDFRPVAVPDD
                     LEIVVVDSGEARTLIGSAYAERRAQAEMAEMVIGPLRDANTLDLQRLDDPLLAARARH
                     VISENARVWAFAEALEGGWPDEAGALMAASHASLRDDYEVSTPVLDNLVERLSARPGV
                     YGARLTGAGFGGCVVALTTPGALSEGWKVRPAAGARVTSA"
     gene            complement(608..1246)
                     /locus_tag="VFP02_07035"
     CDS             complement(608..1246)
                     /locus_tag="VFP02_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET9542818.1"
                     /translation="MAYPSKLLNENETVELDLRPHWWFMVPRLSLLALAVLLGIYAIG
                     TDVSWLDASWFKALAGLLVLAALVFFLLRYALWMTTNFVVTSDRVIYRSGVLSKRGIE
                     VPLDRINTVVFHQGPFERIIGAGDLEIESAGQNPARFTDIRKPSIVQAEIYRQSETNE
                     NRKFDRVGANLEGGLRAAGLSVAEQLEKLEGLRDRGSITEEQYEAQKALLLG"
     gene            complement(1272..1625)
                     /locus_tag="VFP02_07040"
     CDS             complement(1272..1625)
                     /locus_tag="VFP02_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HET9542819.1"
                     /translation="MHVAPERFEDLVADALDDIPEELGALMDNVAVMVVDDPPEPGLL
                     GRYDGVPLTERDDGYGGYGHLPMPDRIEIYRRPLCLMCRTEEEVVEQVRITVVHEVAH
                     HFGIDDDRLDELGWG"
     gene            1671..>2353
                     /locus_tag="VFP02_07045"
     CDS             1671..>2353
                     /locus_tag="VFP02_07045"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HET9542820.1"
                     /translation="MATEGQGDELLGGLDEAQRAAVTSPAGPLCVHAGAGSGKTRVLT
                     RRIAHRAATGDLDPRHALALTFTRKAAGELRARLRDLGLRDDVAAGTFHAIAYAQLRA
                     RWADRGAEVPRLMDRKLRFVADLLGRRRDAAQDVVGEIEWASARLISPSDYLERAARA
                     GRRPPLPGDEMPHLLDRYAEAKTESRVIDFDDLLRLALRIMERDAPAAAAQRWRFRHL
                     FVDEFQDVNP"
BASE COUNT          324 a          850 c          838 g          341 t
ORIGIN      
        1 gccgagcagg ccgcgtccgg cgtcccgtcc gggatcatgg accagctggc ctcggccgcg
       61 ggtgtggaga accacgccct cctcgtcgac tgccgctcgc tcgacttccg gcctgtggcc
      121 gtgccggatg acctcgagat cgtcgtggtc gactcgggcg aggcccgcac gctgatcggc
      181 tccgcgtacg ccgagcgccg ggcccaggcc gagatggccg agatggtcat cggcccgctc
      241 cgggatgcca acacgctcga cctccagcgg ctggacgatc cgctcctggc cgcccgggcc
      301 cggcacgtga tcagcgagaa cgctcgggtg tgggcgttcg ccgaggccct cgaagggggt
      361 tggccggacg aggcgggtgc cctgatggcg gccagtcacg ccagcctgcg cgacgactac
      421 gaggtctcga ccccggtgct cgacaacctg gtcgaacgct tgtcagcacg gcccggggtg
      481 tacggcgccc gcctcaccgg cgccggcttc ggcggctgtg tcgtcgccct cacgaccccc
      541 ggtgcgctca gcgaggggtg gaaggtccgc ccggcggccg gcgcccgggt gacgtccgcc
      601 tgatccgtca gccgaggagc agggccttct gcgcctcata ctgctcctcg gtgatggaac
      661 cgcggtcccg caggccctcg agcttctcga gttgctccgc gacgctgagc ccggcggccc
      721 tgaggccacc ctcgaggttg gccccgaccc gatcgaattt gcggttctcg ttggtctcgc
      781 tctgcctgta gatctcggcc tgcacgatgg acggcttgcg gatgtcggtg aaccgggccg
      841 ggttctggcc cgccgactcg atctccaggt cgccggcgcc gatgatgcgc tcgaacggcc
      901 cctggtggaa caccacggtg ttgatgcggt cgaggggcac ctcgatgccc cgcttcgaca
      961 ggacgccgct gcggtagatg acccggtcgc tggtgaccac gaagttggtg gtcatccaga
     1021 gggcgtagcg gaggaggaag aagaccaggg cggcgaggac gaggaggccg gcgagggcct
     1081 tgaaccagga ggcgtcgagc caggagacat cggtgccgat ggcgtagatc ccgaggagca
     1141 cggccagggc caggaggctg agacggggga ccatgaacca ccagtgcggg cgcaggtcca
     1201 gctcgacggt ctcgttctcg ttcaggagct tgctcggata ggccacggcc gggcagcgta
     1261 ctcctggccg ttcagcccca accgagttcg tcgagccggt cgtcgtcgat cccgaagtga
     1321 tgggccacct cgtggaccac ggtgatccgg acctgctcga cgacctcttc ttcggtgcgg
     1381 cacatcaggc acaacgggcg ccggtagatc tcgatgcggt ccggcatggg caggtgcccg
     1441 tagccgccgt acccgtcatc ccgctcggtc agcgggaccc cgtcgtagcg cccgagcagg
     1501 ccaggctcgg gcgggtcgtc gacgaccatg acggcgacgt tgtccatgag cgcgccgagc
     1561 tcctcgggga tgtcgtcgag ggcgtcagcc accaggtcct cgaagcgttc gggggcgacg
     1621 tgcacggagc cggaacctat cgcccggccc gccggccgta cgctccgtgg gtggcgaccg
     1681 aagggcaggg tgacgagctg ctgggaggcc tcgacgaggc ccagcgggcg gcggtcacca
     1741 gcccggccgg gcccctctgc gtccacgccg gggccggatc gggcaagacc cgggtcctga
     1801 cccgccgcat cgctcatcgg gccgccaccg gcgacctcga cccccggcac gccttggccc
     1861 tgaccttcac ccgcaaggcc gcgggggagt tgcgggcccg cctgcgggac ctcggcctcc
     1921 gcgacgacgt cgccgccggg accttccacg ccatcgccta cgcccagctc cgggcccgct
     1981 gggccgatcg gggagccgag gtcccccggc tcatggaccg caagctccgg ttcgtggccg
     2041 acctgctcgg ccgccgccgg gacgcggccc aggacgtcgt cggtgagatc gagtgggcct
     2101 cggcccggct cataagcccg tccgattacc tggaacgcgc cgcccgggcc ggtcgccgcc
     2161 caccgctgcc cggcgacgag atgccccacc tcctcgaccg ctacgccgag gccaagaccg
     2221 agtcccgggt gatcgacttc gacgacctgc tccggttggc gttgcggatc atggagcggg
     2281 acgccccggc cgccgccgcc cagcgctggc gcttccgcca cctcttcgtc gacgagttcc
     2341 aggacgtcaa ccc
//
LOCUS       DASTFV010000382         3025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1388919, whole genome shotgun sequence.
ACCESSION   DASTFV010000382 DASTFV010000000
VERSION     DASTFV010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388919"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="VFP02_07050"
     CDS             <1..1410
                     /locus_tag="VFP02_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadD3 family acyl-CoA ligase"
                     /protein_id="HET9542821.1"
                     /translation="IAIWGPNCVEWAIAELGLHSAGAVLVPLNTRFKGKEAAYVIGKA
                     RARLLFTVTDFLDTDYVALLDGVEGLYCVEETITLRGASPAGTTPMTEFLARADQVSA
                     DAAAERAAGVAGEDTSDILFTSGTTGNPKGAMLRHAAGIRAYDAWSTVVGLREGDRYL
                     IINPFFHAFGLKAGILASLIKGATMLPQPVFDVPTVMERVPVDRITMLPGPPAIYQTI
                     LNHPDLERFDMSSLRLAVTGAAPVPVEMVNRMYDTLGFETVVTGYGLTEVHGIATMCR
                     HDDDPETIANTDGRAIPDVEVRVVDDEGNEVPRGEPGEVVIRGYNVMSGYFEEPEQTA
                     ETIDADGWLHTGDIAVMDERGYIDITDRMKDMFIQGGFNAYPAEIESLLLDHPDIAQV
                     AVIGVPDERLGEVGMAFVVPTTGTRPDEAAIVAWAREAMANYKAPRYVEIVEALPMNA
                     SNKVLKYELRGQARERLGV"
     gene            complement(1426..2994)
                     /locus_tag="VFP02_07055"
     CDS             complement(1426..2994)
                     /locus_tag="VFP02_07055"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HET9542822.1"
                     /translation="MTTATLPVPVTAARSVRIGGRSWPVILPSLRDSRLHVAATLLTV
                     QVLGQTVIDWDLSITQILVSLATCFILEVTIVARRQGVLAWPASALLTGNGVALLLRV
                     PGTEHGDWWSTRGWAIFAGTAALAVMSKYLIRVGDRPLFNPSNLGLVLCFLVLGSARV
                     EPQDFWWGPWSLGLAVTYAVIIVGGVLVTRRLHLLGVVVSFWVALALALGVVALSGHA
                     ITARWHVGLLTDFAWWWVIVSSPETLIFLFFMITDPRTAPLGRVARVAYGAGVGVVAA
                     LFAAPQQTEFATKVAILAALVAMCAVRPLLERSLPAPGSNEDRWSEVRAGLGRRPRLA
                     VGGALATGLLVVTLILAGGHSRALLPDYELRGERPVVAIGPVPEVAIDDSVADVVLGF
                     ELSEIDLLARDLVEDLEIEAEALRTGDEEVAAQAAVSVRLDDLLAQMDTTGDVTVPHY
                     RFDSLRLILHRELAEGQAAPRLGLEAQGSVRHDRATDGRLTRGDTEAFEAVYLLTEVD
                     GHWLIDDERTVPGS"
BASE COUNT          510 a         1036 c         1100 g          379 t
ORIGIN      
        1 atcgccatct gggggcccaa ctgcgtcgag tgggccatcg ccgagctggg cctgcacagc
       61 gccggggccg tgctcgtgcc actcaacacc cgcttcaagg gcaaggaagc ggcctacgtc
      121 atcggcaagg cccgggcccg cctcttgttc accgtcaccg acttcctgga caccgactac
      181 gtggccctgc tcgacggcgt cgagggcctg tactgcgtgg aggagacgat caccttgcgg
      241 ggggcgtcgc cggccgggac caccccgatg acggagttcc tggcccgggc cgaccaggtc
      301 agcgccgatg ccgcggctga gcgggccgcc ggtgtcgccg gcgaggacac cagcgacatc
      361 ctcttcacct ccggcaccac ggggaacccc aagggggcga tgctccgcca cgccgccggc
      421 atccgggcct acgacgcgtg gtcgacggtg gtcggcctgc gggagggcga ccgctacctc
      481 atcatcaacc ccttcttcca cgccttcggc ctgaaggccg ggatcctcgc cagcctcatc
      541 aagggggcga cgatgctgcc ccagccggtc ttcgacgtgc cgacggtgat ggaacgggtg
      601 ccggtcgacc ggatcacgat gctccccggg ccaccggcga tctaccagac gatcctgaac
      661 cacccggacc tcgagcgctt cgacatgagc tcgctgcgcc tggccgtcac gggcgccgcc
      721 ccggtgccgg tcgagatggt caaccggatg tacgacaccc tcggcttcga gacggtggtc
      781 acgggctatg ggctgaccga ggtccacggc atcgcgacca tgtgccgcca cgacgacgat
      841 cccgagacga tcgccaacac cgacgggcgg gccatccccg atgtcgaggt ccgggtggtc
      901 gacgacgagg gcaacgaggt tcctcggggt gagcccggcg aggtcgtcat ccggggctac
      961 aacgtgatga gcggctactt cgaggagccc gagcagacgg ccgagaccat cgacgccgac
     1021 ggctggctgc acaccggcga catcgcggtg atggacgagc ggggctacat cgacatcacg
     1081 gaccggatga aggacatgtt catccaaggt ggcttcaacg cctacccggc cgagatcgag
     1141 agcctgctcc tggatcatcc cgacatcgcc caggtcgcgg tcatcggggt gcccgacgag
     1201 cgcctcggtg aggtgggcat ggccttcgtg gtcccgacca ccggcacccg cccggacgaa
     1261 gccgcgatcg tggcctgggc ccgggaggcg atggccaact acaaggcccc ccgctacgtg
     1321 gagatcgtcg aggccctccc catgaacgcc agcaacaagg tcctcaagta cgagctgcgg
     1381 ggccaggcgc gggagcggct gggggtgtag gccggcccgg ccgcgtcacg agccggggac
     1441 ggtccgttcg tcgtcaatga gccagtgacc gtcgacctcg gtcagcaggt agacggcctc
     1501 gaaggcctcg gtgtcgcctc gggtgagccg gccgtcggtg gcgcggtcgt ggcgcacgga
     1561 gccctgggct tccagcccca gccggggggc ggcctgtccc tccgccagct cgcggtggag
     1621 gatgaggcgc agggagtcga agcggtagtg aggcacggtc acgtctccgg tcgtgtccat
     1681 ctgggccagg aggtcgtcga ggcggacgct cacggcggcc tgggccgcca cctcctcgtc
     1741 gccggtgcgc agggcttccg cctcgatctc gaggtcctcg accaggtcgc gggccaggag
     1801 gtcgatctcg gacagctcga agccgagaac gacgtcggcc acggaatcgt cgatggcgac
     1861 ctcggggacc ggcccgatgg cgaccaccgg ccgctcgccg cggagctcgt agtcgggcag
     1921 aagggcccgc gaatgccctc cggcgaggat cagggtgacg acgaggaggc cagtggcgag
     1981 cgcgcccccg accgccagcc gtggtcgacg ccccaaccca gcccgcacct cggaccagcg
     2041 gtcctcgttc gagccggggg ccggcagcga ccgctccagg aggggccgca cggcacacat
     2101 cgccaccagc gcggccagga tggccacctt ggtggcgaac tcggtctgct ggggcgcggc
     2161 gaacagggcg gccacgaccc ccaccccggc gccgtaggcc actcgggcca cccggcccag
     2221 cggggcggtg cgcgggtcgg tgatcatgaa gaagaggaag atgagcgtct cgggcgagga
     2281 gacgatgacc caccaccacg cgaagtcggt gagcaggccc acgtgccacc gggccgtgat
     2341 ggcatggccg ctcagggcca cgactcccag ggcgagggcg agggccaccc agaagctgac
     2401 gacgacgccg aggaggtgga ggcggcgggt gacgaggacg ccgcccacga tgatcaccgc
     2461 gtaggtcacg gccagaccaa gtgaccacgg tccccaccag aagtcctggg gctcgacccg
     2521 agcgctgccg aggacgagga agcacaggac gaggcccagg ttggaggggt tgaacaacgg
     2581 tcggtcaccg acccggatga ggtacttcga catcacggcc agggccgcgg tgccggcgaa
     2641 gatggcccag ccccgggtgc tccaccagtc gccgtgctcg gttccgggga cccggaggag
     2701 cagggccacg ccgttcccgg tcagcagggc actggccggc caggccaaca ccccctgacg
     2761 tcgggccacg atcgtgacct cgaggatgaa gcaggtcgcc agcgacacca ggatctgggt
     2821 gatggacagg tcccagtcga tcaccgtctg cccgaggacc tgaacggtca gcagggtggc
     2881 ggcgacgtgg agacggctgt cccgtagaga cgggaggatg acgggccaag agcggccgcc
     2941 gatccgcacg gaacgggcgg cggtgaccgg gacgggcagg gtggccgtcg tcaaggcatc
     3001 gaccctagag gtcggacggc ggcgg
//
LOCUS       DASTFV010000383         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1390581, whole genome shotgun sequence.
ACCESSION   DASTFV010000383 DASTFV010000000
VERSION     DASTFV010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390581"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFP02_07060"
     CDS             <1..272
                     /locus_tag="VFP02_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542823.1"
                     /translation="RQKEGHRLQKEELRKWEQRRRSRRRFLTWGAVIAAAVALLFGYS
                     ALQGDDENESPTTTAPVTTTIAPATDTTAVTSTTAVTPTTSTPAP"
     gene            complement(284..952)
                     /locus_tag="VFP02_07065"
     CDS             complement(284..952)
                     /locus_tag="VFP02_07065"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HET9542824.1"
                     /translation="MSGILALVGGRPFTEGCTFDADLLAASGGDEVVILPTAAAYEHP
                     EREVESATAHFSVLGGRVTGLDVLTRRDALDADLAARVRDARFLYLVGGSPMHLRSVV
                     KDSPVWDALVAAWEDGAVVAAASGAARVLCDPMVDPRGGAFTVGLGLLDNLAVVPHHN
                     HWSEEKAHRTLLLAPAGLLVVGIDDATALLRAPDGTWQVAGAGSVAVFRSREDVGLEA
                     LPTA"
     gene            complement(949..1473)
                     /locus_tag="VFP02_07070"
     CDS             complement(949..1473)
                     /locus_tag="VFP02_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542825.1"
                     /translation="MTVADSILPQGYLRTLPPRARTVIREHRRRLLRDLSGRVLDLGG
                     DPGHGPLYPASTEVVVGDGDGVFDHVVSILHLTAAADPGAELARVRERLAPDGSLVFL
                     EPEIDTGLAARGQRLVAPLVGRFAGWRPDRDVPALLRDARLVMSDLVRTPMPRHLWPL
                     TDLVEGRAHHRINR"
     gene            1588..>2328
                     /locus_tag="VFP02_07075"
     CDS             1588..>2328
                     /locus_tag="VFP02_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET9542826.1"
                     /translation="MDIKRVGIVGSGIMGAGIAQVAAQAGHEVVLRSRQQATADATLA
                     SLEKGLNRLVEKGRLEEVDRDATLSRITATSVLKDLADCDLVIESVVEDLATKKALFV
                     ELNQVCRDDAILATNTSTLPVIEMAVETHNPGRVCGIHFFNPAPTMNLVEIIRPLTAS
                     DETIATAKAFAEDCGKDPVEVKDQAGFIVNALLFPYLNNAVRLLENGTANREDIDTAM
                     KGGCNFPMGPLELLDLVGLDTSVAILDAL"
BASE COUNT          385 a          827 c          836 g          280 t
ORIGIN      
        1 aacggcagaa ggaaggacac cgcctccaga aggaggagct gcgcaagtgg gagcagcgac
       61 gtcggagccg gcgccgtttc ctcacctggg gcgccgtgat cgcggccgcg gtcgctctgt
      121 tgttcgggta ctccgccctc cagggggacg acgagaacga gtcgccgacc accacggcgc
      181 ccgtcacgac cacgatcgcg cccgccaccg acaccaccgc ggtgacgagc accacggccg
      241 tcaccccgac cacgtccacg cccgcgccct agccaggggc ggatcaagcc gtcggcaggg
      301 cctcgaggcc gacgtcctcg cgggaccgga acaccgcgac cgagccggcg ccggcgacct
      361 gccaggtgcc gtccggggcc cgcaggagag cggtggcatc gtcgatgccg accacgagca
      421 gcccagcggg ggccagcagc agcgtgcggt gggccttctc ctcggaccag tggttgtggt
      481 ggggaacgac ggccaggttg tcgagcaggc cgaggcccac cgtgaaggcc ccgccgcggg
      541 ggtcgaccat gggatcgcac aggacccggg cggccccgct ggccgcggcg accactgcgc
      601 cgtcctccca cgccgcgacc agcgcgtccc agaccgggct gtccttgacg accgagcgca
      661 ggtgcatggg cgagccgccg accaggtaga ggaagcgggc gtcgcggacg cgggccgcga
      721 ggtccgcgtc cagggcatcg cggcgggtga gcacgtcgag gcccgtgacc cggccaccga
      781 ggaccgagaa gtgggcggtg gcggactcga cctcgcgctc ggggtgctcg taggcggcgg
      841 cggtgggcag gatcacgacc tcgtcgcccc cgctggcggc cagcagatcg gcgtcgaacg
      901 tgcagccctc ggtgaacggc cggcccccga ccaacgccag gatcccgctc accggttgat
      961 ccgatggtgg gctcgaccct cgaccagatc ggtcaggggc cagaggtggc ggggcatcgg
     1021 cgtgcggacc aggtcgctca tgaccagacg agcgtcgcgc aacagggcgg gcacatcgcg
     1081 atcgggccgc caaccggcga accggccgac gaggggggcg accaggcgct ggccgcgggc
     1141 ggcgagcccg gtgtcgatct ccggctccag gaagaccagg gagccgtccg gagccaggcg
     1201 ctcccgcacc cgagccagct ccgcgccggg atcggccgcg gccgtcaggt gcaggatcga
     1261 caccacgtgg tcgaagacgc cgtcgccgtc gccgaccacg acctcggtcg acgccgggta
     1321 gagcggcccg tgaccggggt cgccgcccag gtccaacacg cgtccgctga ggtcgcgcag
     1381 gaggcgacga cgatgctccc ggatcaccgt ccgggcccgg ggcggcaggg tgcgcaggta
     1441 gccctgagga aggatcgagt cggccacggt cactcgccgc gaaggtatcg gccgtcagaa
     1501 acgctgcgca gcggagccgg gctcgcgagg caggcgaggc cggtcaaatg tgacggcaag
     1561 ctacccatcg gtaacatctg cggccctatg gacatcaagc gtgtggggat cgtcgggtcg
     1621 gggatcatgg gggcgggcat cgctcaggtg gccgcccagg ccggccacga ggtcgtgctg
     1681 cggtcccgtc agcaggccac ggccgacgcc acgctggcct cgttggagaa aggcctcaac
     1741 cgtctggtcg agaagggccg tctcgaggag gtcgaccggg acgccaccct ctcccgcatc
     1801 acggccacct ccgtgctcaa ggacctggcc gactgtgacc tcgtcatcga gtcggtggtc
     1861 gaggatctgg ccaccaagaa ggccctgttc gtcgagctca accaggtctg ccgcgacgac
     1921 gcgatcctgg ccacgaacac ctcgaccctg ccggtcatcg agatggcggt cgagacccac
     1981 aaccccggtc gggtgtgcgg catccacttc ttcaaccccg cgccgaccat gaacctggtc
     2041 gagatcatcc gcccgctgac ggcgtccgac gagaccatcg ccacggccaa ggcgttcgcc
     2101 gaggactgtg gcaaggaccc cgtcgaggtc aaggaccagg ccggcttcat cgtgaacgcc
     2161 ctcctcttcc cgtatctcaa caacgccgtg cgcctgctgg agaacggcac ggccaaccgg
     2221 gaggacatcg acacggccat gaagggtggc tgcaacttcc cgatgggccc gctggagctg
     2281 ctcgacctgg tcggcctgga cacgtccgtg gcgatcctcg acgccctc
//
LOCUS       DASTFV010000384         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1395415, whole genome shotgun sequence.
ACCESSION   DASTFV010000384 DASTFV010000000
VERSION     DASTFV010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395415"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VFP02_07080"
     CDS             complement(<1..913)
                     /locus_tag="VFP02_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET9542827.1"
                     /translation="MAITEERPLALPAAADGDDDFQPLGVFRRPATTTGWRSWVTTVD
                     HKRIGIMYGVAAFFFFCVGGSEALLIRTQLARPGSDLLSANTYNQVFTMHGVTMVFLV
                     IMPMAAAFANYLLPLQIGARDVAFPRMNAFSLWCFLAAGIFVNTSWFLGGGADGGWFA
                     YAPNTGVVFSPSHGLDFFSLGLLIAGIASLASAVNLTVTVLNMRAPGMDLMKMPVFTW
                     MILVVQVLLVLALPVITVALVLLTLDRLFGANFFNVSQGADPLLWQHLFWIFGHPEVY
                     ILILPSFGIVSEVIPVFSRKPIFGYPFM"
     gene            complement(921..2063)
                     /gene="coxB"
                     /locus_tag="VFP02_07085"
     CDS             complement(921..2063)
                     /gene="coxB"
                     /locus_tag="VFP02_07085"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET9542828.1"
                     /translation="MRSARLRTLALGLLLVVVAAGCASDAPLDTLDPKGPEAQSIDNL
                     LLPVMLIAGVVFLFVELGIVYLVVRFRRRKRDDDSELPSQTHGNLVLELGWTILPAVI
                     LAFVAVGTLTTLFDIDDEPEDALEIDVVGQQWWWDFQYDVDGDGAYDFRTANEMIIPA
                     GEPVNLDITSMDVIHSFWIPALNGKKDAVPGRHHPLTLEADEPGTYWGQCTEFCGLSH
                     ANMRIRVIALPQDEYDAWLENQMRPAEAATGDAVDGEELFSSTCTSCHQIRGLNDDYT
                     GAATVSGTAPELTHLMTRSTFAGSIFRLYAQADDADLHDFYTHLAEEGTFNRVQLEAW
                     LRDPPAEKPMYADTATLNGALPRGMPDLELSEVQIDQLVAYLQTLD"
     gene            complement(2113..2880)
                     /locus_tag="VFP02_07090"
     CDS             complement(2113..2880)
                     /locus_tag="VFP02_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542829.1"
                     /translation="MVGTGSRLFFAYTVLALLGAAVYGLASGGDVVGVLTLGYKGGVG
                     EHFGYAVLVLLGASTLVLGLFSVVLRDADPDATAALQASDVLPEVAPPASASPWPLVG
                     AFGLMVTALGLVVGPGLFVLGLILLAVTALEWAVQVWADRATGDPEVNRAIRNRLMAP
                     LEIPVGAVLVILFVVLGISRVLLAVSPLTAVVVASAAASLIMVGAIIVFIRPRHSGTL
                     ATAFLLLGALAIIGGGIAGVAAGERDFHEEPAHEAGE"
BASE COUNT          527 a          918 c         1114 g          402 t
ORIGIN      
        1 ccatgaacgg gtacccgaag atgggcttgc ggctgaagac cgggatgacc tcggagacga
       61 tgccgaagct gggcaggatc aggatgtaga cctcggggtg gccgaagatc cagaacaggt
      121 gctgccacag gagcgggtcg gcgccctggg agacgttgaa gaagttggcg ccgaagagcc
      181 ggtccagggt cagcaggacc agggccaccg tgatgaccgg cagggccagc accaggagca
      241 cctggacgac caggatcatc caggtgaaca ccggcatctt catcaggtcc atccccggcg
      301 cccgcatgtt gagcacggtg acggtcaggt tgacggcgct ggccagcgag gcgatgccgg
      361 cgatgagcag gccgaggctg aagaagtcga ggccgtggga cggcgagaag acgacgccgg
      421 tgttcggggc gtaggcgaac cagcccccgt cggcgccacc gccgaggaac cagctggtgt
      481 tgacgaagat gccggcggcc aggaagcacc acaggctgaa ggcgttcatc cgggggaagg
      541 cgacgtccct ggcaccgatc tggagaggta gcaggtagtt ggcgaacgcc gcggccatgg
      601 gcatgatcac caggaacacc atggtgacgc cgtgcatggt gaacacctgg ttgtaggtgt
      661 tggccgagag caggtcgctg cccgggcggg ccagctgcgt gcggatgagc agggcctcgc
      721 tgccgccgac gcagaagaag aagaaggcgg cgacgccgta catgatgccg atgcgcttgt
      781 ggtcgacggt ggtgacccag ctccgccagc cggtggtcgt cgccgggcga cggaacacac
      841 ccagcggctg gaagtcgtcg tcgccgtcag ctgcggcggg cagagcgagt gggcgttctt
      901 cggtgattgc cacgggtgcc tcagtccagg gtctgtaggt aggccacgag ctgatcgatc
      961 tgcacctcgc tgagctcgag gtcgggcatg ccacggggga gcgcgccgtt cagggtggcg
     1021 gtgtcggcgt acatgggctt ctcggcgggc gggtcgcgca accaggcctc gagctgcacc
     1081 cggttgaagg tgccctcctc ggccaggtgg gtgtagaagt cgtgcaggtc cgcgtcatcg
     1141 gcctgcgcgt agaggcggaa gatggagccg gcgaaggtcg accgcgtcat gaggtgggtc
     1201 agctccggcg cggtgcccga gacggtcgcc gccccggtgt agtcgtcgtt gaggccgcgg
     1261 atctggtggc aggaggtgca ggtcgacgag aacagctctt cgccgtccac cgcgtcaccg
     1321 gtggcggcct cggccggccg catctggttc tccagccacg cgtcgtactc gtcctggggc
     1381 agggcgatga cccggatgcg catgttggcg tgcgacagcc cgcagaactc ggtgcactgg
     1441 ccccagtagg tcccgggctc gtcggcctcc aacgtcagcg ggtggtgccg gccgggcacg
     1501 gcgtccttct tgccgttcag ggcggggatc cagaagctgt ggatcacgtc catcgaggtg
     1561 atgtcgaggt tgaccggttc cccggcgggg atgatcatct cgttcgcggt gcggaagtcg
     1621 taggccccgt caccgtcgac gtcgtactgg aagtcccacc accactgttg gcccacgacg
     1681 tcgatctcga gggcgtcctc gggctcatcg tcgatgtcga acagggtggt cagggtgccc
     1741 acggccacga aggccaggat gaccgcgggc aggatcgtcc agcccagctc gagcacgagg
     1801 ttgccgtggg tctggctggg cagctccgag tcgtcgtcgc gcttgcgcct ccggaagcgg
     1861 acgacgaggt agacgatgcc cagctcgacg aacaggaaca cgaccccggc gatgagcatg
     1921 accggcagga gcaggttgtc gatcgactgg gcctcggggc ccttcgggtc cagcgtgtcc
     1981 aggggcgcgt cgctggcgca gccggcggcg accaccacca ggaggaggcc aagggccagg
     2041 gtgcgcaggc gggcggacct caccggccga gtgtgaccca ccggatcggg gggttccaag
     2101 tcggtcaccg ccctactcac cggcctcgtg agccggctct tcgtggaagt cgcgctcgcc
     2161 cgcggccacg ccggcgatgc caccgccgat gatggccagg gcgcccagca ggaggaacgc
     2221 ggtggccagc gtgccgctgt gccgggggcg gatgaacacg atgatcgccc cgaccatgat
     2281 caggctggcg gcggccgaag cgacgacgac cgcggtcagg ggtgacacgg cgagcagcac
     2341 ccgggagata ccgaggacga cgaagaggat gaccaggacc gcgccgacgg ggatctcgag
     2401 cggcgccatg agccggttgc ggatcgcccg gttcacctcg ggatcacccg tcgcccggtc
     2461 ggcccacacc tgaacggccc actcgagggc ggtgacggca agcaggatca gaccgaggac
     2521 gaagaggccg ggcccgacga ccaggccgag ggcggtgacc atgaggccga aggcgccgac
     2581 caacggccag gggctggccg aggcgggcgg cgccacctcg ggcaggacgt cgctggcttg
     2641 gagcgccgcg gtggcatccg gatccgcgtc tcgcaggacg acgctgaaca acccgaggac
     2701 cagggtcgag gccccgagga ggacgagcac ggcgtacccg aagtgctcac cgaccccgcc
     2761 cttgtagccg agggtgagaa cgcccacgac atcgccacca ctggcgaggc cgtacaccgc
     2821 cgccccgaga agggcgagga cggtgtaggc gaagaacagg cgagagccgg taccaaccat
     2881 gagctcctac ttggtcacgt agacggcgta ccagatcacg atgtagatcg cgaccgaggc
     2941 gttccagaag atggcggcgg c
//
LOCUS       DASTFV010000385         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1397229, whole genome shotgun sequence.
ACCESSION   DASTFV010000385 DASTFV010000000
VERSION     DASTFV010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397229"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2523)
                     /locus_tag="VFP02_07095"
     CDS             complement(<1..>2523)
                     /locus_tag="VFP02_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET9542830.1"
                     /translation="EARARVRAVLADVDEITITTLHGFASRLLGEFPLEAGLPPGFEV
                     EDEIEASVRFDRRWVGLVDDLHTDPDLREVLGRALVLGLPLRRLAEVARVFDDNWDRL
                     GPARDRTALSPVDVAAVAEPLAAALDHLDACVDPDDLLCEHLENHAGPIHRELALLTE
                     VGDPEALLTALVRAGKLSSKSGRQEAWHGAKPDIVAALEAAEDARVALVHAHRSEVLG
                     ILTDRLRRFALEGADDRRGRGLVSYHDLLVRARDLLRADGDVRVALARRYRYLLIDEF
                     QDTDPLQIEIAALLAAADPAHVPERWQDVDVVAGKLFFVGDPKQSIYRFRRADIALYA
                     QAEEAFAADPVRLTANFRSVPDVLSWVNEVFGELIADDGDAQPPYVDLAPHRRAPGTR
                     PSVLVVGGPVENRSIGDIRDDESEELARLIRRMRAEEWEVGRTERAPLRYDDVALLVP
                     TRTPVGQVERALEGEGIPYRIESRSLVFQTDEVRELLALLAAVDDPADEVALITALRS
                     PGLGCSDADLADWRLAGGQWRVDGTVPEGLEEHRVASALQEIARLHGERDWKPVNELV
                     AQVVRERRLVELTLARRRPRDHWRRYRFLTDAARAFVEAGGASLAEFVAWVTAQAEGG
                     ADRLETVVREADDDAVRIMTVHGSKGLEFPVVVLAGLNADRDSRSPVVTWGPERPEVR
                     FGRKGDYFETAGYLDVREADKRFEEAEQRRLLYVAATRAQDHLVVSLHHKAGYPSHAA
                     LLHEVCQAKAGAATPPEDTQLALPLTVDATVPTEQPVEGQEVAEDWRRARLELLGRVR
                     ASGVATPSGLGHDEPLPVPEEDQRVPGRGEGGTARGRAVHAVL"
BASE COUNT          322 a          923 c          954 g          324 t
ORIGIN      
        1 caggacggcg tggacggcgc gaccgcgggc cgtgcccccc tcgccccgcc cggggacgcg
       61 ctggtcctcc tcggggacgg gcagtggctc gtcgtggccg aggccgctcg gcgtcgccac
      121 ccccgaggcc cggacccggc ccaggagctc gagccgggcc cgccgccagt cctccgccac
      181 ctcctggccc tccacgggtt gctcggtcgg gacggtggcg tccacggtca ggggcagggc
      241 gagctgggtg tcctcgggcg gggtcgcagc cccggccttg gcctggcaga cctcgtggag
      301 cagcgcggcg tggctcgggt agcccgcctt gtggtggagg ctgaccacga ggtggtcctg
      361 ggcccgggtg gcggcgacgt acagcagccg acgctgctcg gcctcctcga agcgcttgtc
      421 ggcctcgcgg acgtcgaggt agccggccgt ctcgaagtag tcacccttcc gcccgaatcg
      481 gacctccggt cgctccggcc cccaggtcac gaccggcgag cgggagtccc ggtcggcgtt
      541 gaggccggcc agcaccacca ccgggaactc caggcccttc gagccgtgca cggtcatgat
      601 gcggacggcg tcgtcgtcgg cctcccggac cacggtctcg aggcggtcgg cgccgccttc
      661 ggcctgcgcc gtgacccagg ccacgaactc ggccagggac gcgcccccgg cctcgacgaa
      721 ggcccgcgcc gcgtcggtca ggaaccggta gcggcgccag tggtcccggg gacgccgacg
      781 ggccagggtc agctcgacca gccggcgctc ccgcaccacc tgggcgacca gctcgttgac
      841 cggcttccag tcccgctcgc cgtggaggcg ggcgatctcc tgcagggccg acgccacgcg
      901 gtgctcctcg aggccttcgg gcacggtccc gtcgacgcgc cactgtccac cggcgaggcg
      961 ccagtcggcg aggtcggcgt cggagcagcc caggcccggt gagcgcaggg cggtgatcag
     1021 ggcgacctcg tcggcggggt cgtcgaccgc ggccaggagg gccaggagct cacgcacctc
     1081 gtcggtctgg aagacgagcg accggctctc gatccggtag gggatgcctt cgccctccag
     1141 ggcgcgctcc acctgtccga ccggtgtgcg ggtgggcacg aggagggcga cgtcgtcgta
     1201 gcgcagcggg gcccgttcgg tccggccgac ctcccactcc tcggcccgca tccgccggat
     1261 cagccgggcc agctcctcgg actcgtcgtc gcggatgtcg ccgatcgagc ggttctcgac
     1321 cggacccccg acgaccagga ccgaggggcg ggtgcccggt gcccgccggt gcggggccag
     1381 gtccacgtag ggaggctgag catcgccgtc gtcggcgatg agctcgccga acacctcgtt
     1441 cacccacgac aggacgtccg gcacggagcg gaagttggcc gtgagccgga ccgggtcggc
     1501 ggcgaaggcc tcctccgcct gcgcgtagag cgcgatgtcc gcccgccgga agcggtagat
     1561 ggactgcttc gggtcgccga cgaagaacag cttcccggcg acgacgtcga cgtcctgcca
     1621 gcgctcgggg acgtgagccg gatcggccgc ggccagcagg gcggcgatct cgatctgcag
     1681 cgggtcggtg tcctggaact cgtcgatgag caggtaccgg tagcgccggg ccagggccac
     1741 ccgtacgtcg ccgtcggcgc gcagcaggtc tcgggcccgc acgaggaggt cgtggtacga
     1801 caccaggccc cggccgcggc ggtcgtcggc cccttccagc gcgaagcggc gcaagcgatc
     1861 ggtcaggatg ccgagcacct cgctgcggtg ggcgtggacg agggcgacac gagcgtcctc
     1921 ggctgcttcg agggcggcga cgatgtcggg cttggccccg tgccaggcct cctggcgacc
     1981 gctcttgctg ctgagcttgc cggcccggac cagcgcggtg agcagcgcct cggggtcccc
     2041 cacctcggtc aggagggcca gctcccggtg gatcggcccg gcgtggttct ccaggtgctc
     2101 gcagagcaga tcgtccgggt ccacgcaggc gtcgaggtga tcgagggcgg ccgccagggg
     2161 ctccgcgacg gcggcgacgt cgaccgggct caaggccgtg cggtcccggg ccgggcccag
     2221 ccggtcccag ttgtcgtcga agacccgggc cacctcggcc aagcggcgca gcgggaggcc
     2281 gagcaccaag gcccggccga ggacctctcg gaggtcggga tccgtgtgca ggtcgtccac
     2341 cagtccgacc cagcggcggt cgaagcgcac cgaggcctcg atctcgtcct ccacctcgaa
     2401 gccggggggc aaccccgcct ccaacgggaa ctcgccgagg aggcggctgg cgaagccgtg
     2461 cagcgtcgtg atcgtgatct cgtcgacgtc ggccagcacc gcgcggaccc gggcgcgggc
     2521 ctc
//
LOCUS       DASTFV010000386         2397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1408201, whole genome shotgun sequence.
ACCESSION   DASTFV010000386 DASTFV010000000
VERSION     DASTFV010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408201"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFP02_07100"
     CDS             <1..588
                     /locus_tag="VFP02_07100"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET9542831.1"
                     /translation="RVAHTRVDDQCLFGIVQGGVDVDLRAESAERTVALEFDGYGIGG
                     LSVGETLAEMLPALDATTTRLPADQPRYLMGVGDPVGLLEGIARGVDLFDCVLPSRLG
                     RHGTILTGTGRLNLRNQRFGDDAGPLDPDCPCGVCARYSRAYLRHLLKTDEPTGPRLL
                     TLHNLAWLLDLVHRAGAAIRAGTLADLRAEVSRHW"
     gene            758..1042
                     /gene="yajC"
                     /locus_tag="VFP02_07105"
     CDS             758..1042
                     /gene="yajC"
                     /locus_tag="VFP02_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET9542832.1"
                     /translation="MANLILLPLFFAVFYFLLIRPQQKRAKEHTDLISSLEAGDDVLT
                     TGGIYGTIVSIDGQIAELEIADGVIVRIQRSAVAELIEWDTGDASSDEDQ"
     gene            1049..>2397
                     /locus_tag="VFP02_07110"
     CDS             1049..>2397
                     /locus_tag="VFP02_07110"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542833.1"
                     /translation="MRRKGVLPLVFFVVFTLGTLAWTFIVGNSPVLGLDLQGGVSVVL
                     QPDGPVENEDTLNQAVDIIRNRIDALGVAEPDITRQGETVLVQLPGVENQDRVLEVIG
                     QTAVLSFRPVLQTLPPVGQEVPTTTGAPTTTAGDGATTTTGPGGATTTTAPSTSTTEE
                     LGLGAPGALTPGENAAGLQATTTTVAPTTTGAPGTTVPGSTATTVPADGAGAGECAGV
                     EDQARLRPNGVPRGLNDEGVTPSDEVEPCNTVVLAERDEDGNVVTRYVLGPTALTGDA
                     LSGAAAGLDQSGVTWLVRPTFRGGEDGIDKFNAAAAQCFSAAPTCPTTQLAIVLDDDV
                     ISAPQIQTDSFERDQIQISGDFDESSAKDLALVLEYGALPVELVPQQTQTVSATLGED
                     SLRAGLVAGIIGLGLTVIFMVGYYRLLGVVAVVSLTLEFALVWVLISYLGESRGLALT
                     "
BASE COUNT          350 a          899 c          806 g          342 t
ORIGIN      
        1 cgcgtcgccc acacccgggt cgatgaccag tgcctcttcg gcatcgtgca gggcggggtc
       61 gacgtcgacc tgcgggccga gagcgccgag cggaccgtgg ccctggagtt cgacggctac
      121 ggcatcggtg ggctctcggt cggggagacg ctggccgaga tgctcccggc gctggacgcc
      181 accacgaccc ggctcccggc ggatcagccc cgctacctca tgggggtcgg cgaccccgtg
      241 gggctgctgg agggcatcgc ccggggcgtg gatctcttcg actgcgtgct gcccagccgc
      301 ctcggccggc acggcaccat cctgaccggg acgggccggc tcaacctgag gaaccaacgc
      361 ttcggggacg acgccggacc gctcgacccc gactgcccct gcggggtctg cgcccgctat
      421 tcccgggcct atctgcgcca cctgctcaag accgacgagc ccaccgggcc ccgcctcctg
      481 accctgcaca acctggcctg gctcctggat ctcgtgcacc gggcgggggc ggccatccgg
      541 gccgggaccc tggccgacct tcgagcagag gtctcccgcc actggtgagg cggcgcaggg
      601 gccagccgga gcaccggaga ggaccgccgg gatcggcggc gccggacctg ctcagcgcag
      661 cgcagccagt caccggtgac ggcctgaccg gatgaccggc gacccgcgcc gctagagtcg
      721 cccgccgact tcaccgcatc ttgtcggaga cccttgcatg gccaacctca tcctcctgcc
      781 gctgttcttc gcggtcttct acttcctgct catccgcccc cagcagaagc gggccaagga
      841 gcacaccgac ctcatcagct ccctcgaagc gggcgacgat gtcctgacca cgggcggcat
      901 ctacgggacg atcgtctcga tcgacggtca gatcgccgag ctggagatcg ccgacggtgt
      961 catcgtccgc atccagcgca gcgcagtggc cgagctcatc gaatgggaca ccggcgatgc
     1021 ctcttccgac gaggaccagt aaccctccgt gcgccgcaag ggggtcctgc ccctcgtctt
     1081 cttcgtcgtc ttcacgctgg gcacgctggc ctggacgttc atcgtcggga actcgccggt
     1141 gctcggcctc gatctccagg gtggtgtctc cgtcgtgctc cagcccgatg gccccgtcga
     1201 gaacgaggac accctgaacc aggccgtgga catcatccgg aaccggatcg acgccctggg
     1261 tgtcgccgag cccgacatca cccgccaggg cgagaccgtg ctggtgcagc tccctggtgt
     1321 cgagaaccaa gaccgggtct tggaggtgat cggccagacc gccgtcctga gcttccgacc
     1381 ggtgctgcag acgttgccgc ccgtcggcca ggaggtcccc accacgaccg gggcgccgac
     1441 caccacggcg ggcgacgggg cgacgacgac cacgggaccg ggaggggcca cgacgacgac
     1501 ggcgccctcg acgtccacca ccgaggagct cggtcttggc gcgcccggcg ccctgacgcc
     1561 gggggagaac gccgccggtc tccaagccac gaccaccacg gtcgccccca cgacgaccgg
     1621 cgctccggga acgacggtcc cgggctcgac ggccacgacc gtgcccgccg acggggcggg
     1681 cgcgggtgag tgcgccggcg tcgaggacca ggcccgcctc cgtccgaacg gcgtgccccg
     1741 ggggttgaac gacgaagggg tcacgccctc cgacgaggtc gaaccgtgca acaccgtggt
     1801 cctggccgag cgggacgagg acggcaacgt cgtcacccgc tacgtgctgg gcccgaccgc
     1861 gctcaccggt gatgccctgt ccggcgccgc cgccggcctc gaccagagcg gtgtcacctg
     1921 gctggtccgc cccacgtttc gaggcggcga ggatggcatc gacaagttca acgccgccgc
     1981 cgcccagtgt ttctccgcgg cgccgacgtg cccgaccacc cagctggcga tcgtcctcga
     2041 cgacgacgtc atctccgccc cccagatcca gaccgacagc ttcgagcggg accagatcca
     2101 gatcagcggt gacttcgacg agtcctcggc caaggacctg gccctggtgc tcgagtacgg
     2161 ggccctgccg gtcgagctcg tgccccagca gacccagacg gtgtcggcaa cgctgggtga
     2221 ggactccctg cgggccgggc tcgtcgccgg catcatcggc ctgggcctga cggtcatctt
     2281 catggtgggc tactaccgcc tgctgggcgt ggtggccgtc gtcagcctca ccctggaatt
     2341 cgccctcgtg tgggtcctca tctcgtacct cggcgaatcc cggggcctgg ccctgac
//
LOCUS       DASTFV010000387         3205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1413813, whole genome shotgun sequence.
ACCESSION   DASTFV010000387 DASTFV010000000
VERSION     DASTFV010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413813"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFP02_07115"
     CDS             <1..517
                     /locus_tag="VFP02_07115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542834.1"
                     /translation="ESMVAVRGTDAVAVPDGVRALDPAWPGSSGVDLLGTDVVPEGLA
                     ALTAEDLGRLRIEAGVPAMGAELSERTIPAEAGIVERSVSFTKGCFTGQELVARIDSR
                     GGNVPRRLRLLRGAGLTVGAEVVVDGKVVGEVTSAVGDLGLAYIGRAVEPPAAGTVAD
                     APVAIVALPQA"
     gene            514..1320
                     /gene="trpC"
                     /locus_tag="VFP02_07120"
     CDS             514..1320
                     /gene="trpC"
                     /locus_tag="VFP02_07120"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET9542835.1"
                     /translation="MTYLDGIVAAHRERAAADDRARGRVLEQARATAAPRPFREALAA
                     TTGVALIAEIKRRSPSKGDLAPELDPATLARGYEAGGATCLSVLTDGPHFGGSPADLG
                     AARQAVAVPVLRKDFTVDEVDVCDARLMGADAVLLIVAALDDAELRAFAQLAAELDLA
                     ALVEVHDTAEVERALAAGADLIGVNQRDLTTFAVENHRALDLVAQLPAGVVRVAESGI
                     TGPADVIALADGGYDAVLVGETLVTSDDPEATTASLVAAGIGHGGGRGDG"
     gene            1331..1993
                     /locus_tag="VFP02_07125"
     CDS             1331..1993
                     /locus_tag="VFP02_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET9542836.1"
                     /translation="MFVKICGITSEEDALLAVAMGADAVGFVFAPSPRQMAVNKVREI
                     ASRLPPEVLTVGVFKDSSRQQIVRTVQTCGLKAAQLHGRETPELTREVRQRVGLVIKG
                     LPAGDPALDHWDEFGADALLLDAPRPGSGQVFDWSLADGIPVNRRVILAGGLTPENVA
                     GGIEAVHPWGVDVSTGVEQSPGRKDPRKVRAFVSAARAAAPPEYEGSGRAPYDWEAET
                     GW"
     gene            2020..3171
                     /gene="trpB"
                     /locus_tag="VFP02_07130"
     CDS             2020..3171
                     /gene="trpB"
                     /locus_tag="VFP02_07130"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET9542837.1"
                     /translation="MPDPAGDGRFGRFGGRFVPESLVPACEELETAFTEAWADPAFRA
                     ELDGLLTDYAGRPSPLTECHRLGEELGCRLLLKREDLNHTGSHKINNVLGQALLAQRM
                     GKTRLVAETGAGQHGVATATAAALLGMECLVYMGEVDMERQELNVFRMRLLGAEVRGA
                     ASGSRTLKDAINEAMRDWVATVETTHYCLGSVMGPHPYPWMVRELQRVIGVEARAQIL
                     ERTGALPDVVVACVGGGSNAMGLVSGFADDVETELVGVEPAGGAAVGHGVPGVVHGST
                     SYLLQDEAGQVQEAHSVSAGLDYPGVGPEHAHLADIGRARYEAVDDEEVLDAFALLSR
                     TEGIIPALEPAHALAWVARERESLRDRTVLLNLSGRGDKDVAQVAELRG"
BASE COUNT          408 a         1145 c         1227 g          425 t
ORIGIN      
        1 cgagtcgatg gtggccgtcc ggggcaccga tgccgtggcg gtgcccgacg gcgtccgggc
       61 gttggacccg gcctggcccg ggtcgtccgg tgtcgacctg ctgggcaccg acgtcgtgcc
      121 cgagggcctc gccgccctga ccgccgagga cttggggcgc ctgcgcatcg aggcgggcgt
      181 gccggcgatg ggggccgagc tctccgagcg caccatcccg gccgaagccg ggatcgtcga
      241 gcgctcggtg tccttcacca aaggttgctt caccggccag gagctggtgg cccgcataga
      301 cagccggggc ggcaacgtcc cccgccgcct ccgcctgttg cgaggtgccg ggctgacggt
      361 cggcgccgag gtcgtcgtgg acggcaaggt cgtcggcgag gtcacctccg cggttggtga
      421 cctcggcctg gcctacatcg gccgcgcggt cgagcccccc gcggcgggca cggtcgccga
      481 tgccccggtg gccatcgtcg ccctcccgca ggcatgacct acctcgacgg catcgtcgcg
      541 gcccaccggg agcgggccgc ggccgacgat cgagcacggg gcagggtcct ggagcaggcc
      601 cgggcgacgg cggcgccccg tcccttccgg gaggcactgg cggccacgac cggcgtggcc
      661 ctgatcgccg agatcaagcg ccggtcgccc tccaagggcg acctggcccc ggagctggac
      721 cccgcgacgc tggcccgcgg ctacgaggcg ggcggggcca cctgcttgtc ggtgctgacc
      781 gacggccccc acttcggcgg ctcgcccgcc gacctcggcg cggcgcggca ggccgtggcc
      841 gtcccggtcc tgcgcaagga cttcaccgtc gacgaggtcg acgtctgcga cgcccggctg
      901 atgggagccg acgccgtgct gttgatcgtg gccgccctcg atgacgccga gctgcgggcg
      961 ttcgcccagt tggctgccga actggacctg gccgcgctcg tcgaggtcca tgacaccgcc
     1021 gaggtcgagc gggccctggc ggccggcgct gacctgatcg gggtgaacca gcgggacctg
     1081 acgaccttcg ccgtcgagaa ccaccgggca ttggacctgg tcgcccagct gccggcgggc
     1141 gtggtccgcg tggccgagtc cggcatcacc gggccggccg atgtcatcgc cctcgccgat
     1201 ggcggctacg acgccgtcct ggtgggggag acgctggtca cctccgacga ccccgaggcc
     1261 accacggcga gcctggtggc ggccggaatc ggccacggtg gcggccgggg cgacgggtag
     1321 cgtcccggcc gtgttcgtca agatctgcgg gatcacgagc gaggaggacg ccctcctggc
     1381 ggtggccatg ggcgccgatg cggtcggctt cgtcttcgcc ccctcacccc gtcagatggc
     1441 ggtcaacaag gtgcgggaga tcgccagccg gctgcccccg gaggtcctga ccgtgggcgt
     1501 gttcaaggac agcagccgcc agcagatcgt ccggaccgtg cagacgtgtg gcctcaaggc
     1561 ggcccagctc cacgggcggg agacccccga gctcacccgc gaggtccgcc agcgcgtcgg
     1621 cctggtcatc aaagggctgc ccgcgggcga ccccgcgctc gaccactggg acgagttcgg
     1681 cgccgacgcc ctgctgctgg acgcgccccg ccccgggtcc ggccaggtct tcgactggtc
     1741 gctggccgac ggcatcccgg tgaaccgccg ggtgatcctg gccggcggcc tgacgccaga
     1801 gaacgtggcc ggtggcatcg aggccgtcca cccctggggc gtggacgtgt cgaccggggt
     1861 cgagcagtcg ccgggacgca aggatccgcg caaggtgcgg gcgttcgtga gcgcggcccg
     1921 ggccgcggcg ccccccgagt acgaggggag tggccgggcg ccgtacgact gggaggcgga
     1981 gaccggatgg tgaccgacgg gtcgagcgga tcgtcggtga tgccggatcc ggcaggcgat
     2041 ggccgcttcg ggcgcttcgg cgggcgcttc gtgcccgagt cgctcgtccc ggcctgcgag
     2101 gagctggaga cggcgttcac cgaggcctgg gccgatccgg ccttccgggc cgagctggac
     2161 gggctgctga ccgactacgc cgggcgaccg tcgcccctga ccgagtgcca tcgactcggc
     2221 gaggagctgg gctgtcgcct gctgctcaag cgggaggacc tcaaccacac cgggtcccac
     2281 aagatcaaca acgtcctcgg ccaggccctg ctggcccagc ggatgggcaa gacccggctg
     2341 gtggccgaga ccggcgccgg ccaacacggc gtggccacgg cgaccgccgc cgccctcctg
     2401 ggcatggagt gcctcgtcta catgggcgag gtggacatgg agcgccagga gctgaacgtc
     2461 ttccgcatgc ggctgctcgg cgccgaggtg cggggggcgg cgtcgggcag tcggacgctg
     2521 aaggacgcca tcaacgaggc catgcgggac tgggtggcca cggtcgagac cacgcactac
     2581 tgcctcggct cggtcatggg cccccacccg tacccctgga tggtgcggga gctgcaacgg
     2641 gtcatcggcg tcgaggcccg ggcccagatc ctggagcgca ccggcgccct gcccgatgtc
     2701 gtcgtggcct gcgtaggtgg gggctccaat gccatgggcc tggtctccgg gttcgccgac
     2761 gacgtcgaga ccgagttggt cggcgtggag cccgcagggg gcgccgcggt cggccacggg
     2821 gtaccgggtg tggtccacgg cagcacgtcg tacctgctgc aggacgaggc cggtcaggtc
     2881 caggaggccc actcggtgtc ggcggggctg gactacccgg gcgtcggccc cgagcacgcc
     2941 cacctggccg acatcggccg ggcccggtac gaggcggtgg acgacgagga ggtcctcgac
     3001 gccttcgccc tgctgtcccg caccgagggc atcatcccgg ccctggaacc ggcccacgcc
     3061 ctggcctggg tcgcccggga gcgggagtcg ttgcgcgacc gcaccgtcct gctcaacctg
     3121 tccggccggg gggacaagga cgtcgcccag gtcgccgagc tccgtggctg agcgcctgga
     3181 ggatgccctg cgggccggac gggac
//
LOCUS       DASTFV010000388         3775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1419715, whole genome shotgun sequence.
ACCESSION   DASTFV010000388 DASTFV010000000
VERSION     DASTFV010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419715"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1001)
                     /locus_tag="VFP02_07135"
     CDS             complement(102..1001)
                     /locus_tag="VFP02_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HET9542838.1"
                     /translation="MSLPDPRNESVLVYVNGDWVPRADATVSVFDSAFLVGDGVWEGL
                     RLHQGVFPHLDLHLDRLFATAAAVGLDIGKTRGELVDILQETARRNGMRTDVHVRLMV
                     SRGTKKTPSQDPRLVVGGPTIVVIAEHKVADPAVAAVGVDLITAAVRRPPPETLDQRW
                     NSHSKLHEVVALLQAIDAGGDEALMLDVNGHVATCNATNFFIITGGEVWTSTGEHCLN
                     GITRRLVLELAVAAGIGAHERDFTLDDVYRADEAFVTGTFGALTPVAHVDGHAIGDGT
                     GFGPITRQLAGQYREAMDRAARP"
     gene            complement(1062..1757)
                     /gene="cofE"
                     /locus_tag="VFP02_07140"
     CDS             complement(1062..1757)
                     /gene="cofE"
                     /locus_tag="VFP02_07140"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HET9542839.1"
                     /translation="MTIELIAVEGIPEVRPGDDIARLIAAATEVRSGDVLVVTQKIVS
                     KAENQLVAVDPDDPLSHKALVEEESVRVLRRRGDLVISETKHGFVCANAGVDRSNLDD
                     GVAALLPRDPDRSARRIRDRLRDATGAEVGIIVSDTFGRAWRKGLVDVAIGCAGIAAV
                     VDLRGSEDAKGHQLQVTEVAVADEIAAAAELVMGKADGVPIAIVRGLDAGWLRESSVK
                     AEIVRPSSEDLFR"
     gene            complement(1754..2674)
                     /gene="cofD"
                     /locus_tag="VFP02_07145"
     CDS             complement(1754..2674)
                     /gene="cofD"
                     /locus_tag="VFP02_07145"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HET9542840.1"
                     /translation="MTVAVVAGGVGAARLLGGLIQVVDPASITAVVNTGDDVELHGLH
                     ISPDLDTITYTLAGAVNPETGWGLVGETWQAMDALSRYGGVTWFNLGDRDLGTHLFRT
                     QRLREGVSLTEVTAEIVAGWDLGLRLVPMSDDPVRTMITVVDEGEIGFQDYFVRRHHD
                     VVATDIRFAGASTARPAPHALAALADAERVVIAPSNPLVSIAPVLSVPGLREAVEGRR
                     EDCVAVSPIIAGTTVKGPADRLMTELGHDASVVGVARLYAPLASRLVIDEADADLADA
                     VRAEGIDAVVAPTMMYGPPEAAALGRVVLS"
     gene            complement(2671..3651)
                     /gene="gmd"
                     /locus_tag="VFP02_07150"
     CDS             complement(2671..3651)
                     /gene="gmd"
                     /locus_tag="VFP02_07150"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9542841.1"
                     /translation="MPRRALITGITGQDGSYLAEHLLDHGYEVVGMVRRSSTVNFERI
                     AHIQDRITIVPGDVLDEISMIEILSTHRPDEVYNLAAQSFVQTSFNQPVLTGETTALG
                     VTRLLDAIRIVDPDIRFYQASSSEMFGKVQEVPQTESTPFYPRSPYGVAKVYGHWITI
                     NYRESYNLHASSGILFNHESPRRGMEFVTRKISHTVAQIKLGHAEELRLGNLEAQRDW
                     GYAGDYVKAMWQMLQQDEPDNYVVSTNETHSVREFCEVAFDHVDLDWEHYVIMDEAFM
                     RPAEVDLLVGDSAKARSVLGWTPTVDFEDLVTMMVSADLDLLNGKLRGIS"
BASE COUNT          555 a         1256 c         1372 g          592 t
ORIGIN      
        1 ctcgtcgaac acgagcgtgt cggatcgttg gcgccagctg tacatgagcg ccgtggagac
       61 cgtgcgaggg ccgcaccaca tgctgaccac ggtcagcgcg gtcatggccg tgccgctcgg
      121 tccatcgcct cccggtactg gccggcgagc tgccgggtga tcggcccgaa cccggtgccg
      181 tcaccgatgg cgtgaccatc cacgtgggcg accggcgtca acgcgccgaa ggtccccgtg
      241 acgaaggcct cgtcggcgcg gtagacgtcg tccaaggtga agtcccgctc gtgggccccg
      301 atcccagcgg cgacggccag ctcgaggacc aatcgacgag tgatgccgtt gaggcagtgc
      361 tccccggtcg aggtccacac ctcgccgccg gtgatgatga agaagttcgt ggcgttgcac
      421 gtggccacgt gcccgttcac gtccagcatg agcgcctcgt caccgccggc gtcgatggcc
      481 tggaggaggg ccaccacctc gtgcagcttg ctgtggctgt tccagcgctg gtcgagggtc
      541 tcgggcgggg gtcggcgcac cgcggcggtg atgaggtcga cgccgaccgc ggctacggcg
      601 ggatcggcga ccttgtgctc ggcgatgacc acgatggtcg ggccgccgac gacgaggcgc
      661 gggtcttgag acggcgtctt cttggtgccg cgggagacca tgaggcggac gtggacgtcg
      721 gtgcgcatgc cgttgcgccg ggcggtctcc tggaggatgt cgaccagctc ccccctcgtc
      781 ttgccgatgt cgaggccgac ggctgccgcg gtggcgaaca accggtcgag gtggaggtcg
      841 aggtggggga agacgccctg gtggagtcgg agcccctccc acaccccgtc cccgaccaga
      901 aacgcggaat cgaagacgga caccgtcgcg tcggctcgtg gcacccagtc gccgttgacg
      961 tagacgagga ccgactcgtt gcgggggtcg ggcagggaca cgtgggtccc cttcatgacg
     1021 ggtggccacc catggtcatc ggacgatcat gccgtcgacg gctaccggaa gaggtcctca
     1081 ctcgaggggc ggacgatctc ggccttgacg ctggactccc gcagccagcc ggcatccagg
     1141 ccccgcacga tggcgatggg gacgccgtcg gccttgccca tgaccagctc ggcggctgcc
     1201 gcgatctcgt cggcgaccgc gacctcggtg acctggagct ggtggccctt ggcgtcctcc
     1261 gagccccgta ggtcgaccac ggcggcgatg ccggcgcagc cgatggccac gtcgaccagg
     1321 cccttgcgcc aggcccggcc gaacgtgtcg gagacgatga tcccgacctc ggcgccggtg
     1381 gcgtcccgga gccggtctcg aatccggcga gccgagcggt cagggtcgcg ggggagcagc
     1441 gccgccacgc cgtcatccag gttggaccgg tcgacgcccg cgttggcgca gacgaagccg
     1501 tgcttggtct cgctgatgac cagatcgccg cggcgacgga gcacccgcac cgactcctcc
     1561 tcgacgaggg ccttgtgcga gagggggtcg tcggggtcga cggccaccag ctggttctcg
     1621 gccttggaca cgatcttctg cgtgaccacg agcacgtcgc cgctgcggac ctcggtggcg
     1681 gcggcgatga gcctggcgat gtcatcaccg gggcggacct cggggatgcc ctcgacggcg
     1741 atcagctcga tggtcacgag agcaccacgc gccccagggc ggccgcttca ggagggccgt
     1801 acatcatggt cggggccacc acggcgtcga tgccctcggc tcggacggcg tcggcgaggt
     1861 cggcgtcggc ttcgtcgatg accagtcggg aggccaacgg ggcgtagagg cgggccacgc
     1921 cgaccacgga ggcgtcgtgg cccagttcgg tcatcagtcg gtcagccggg cccttgaccg
     1981 tggtcccggc gatgatgggg gacaccgcca cgcagtcctc ccgccggccc tcgaccgcct
     2041 cccgcagtcc ggggacggac agcaccgggg cgatggacac cagggggttg gaaggggcga
     2101 tgaccacccg ttcggcgtcg gccagggcgg cgagggcgtg gggggccggc cgcgccgtcg
     2161 acgctccggc gaagcggatg tcggtggcga ccacgtcgtg gtggcggcgc acgaagtagt
     2221 cctggaagcc gatctcgccc tcgtccacga cggtgatcat ggtccgtacg ggatcgtcgc
     2281 tcatgggcac gagacgcagg ccgaggtccc agccggcgac gatctcggcc gtgacctcgg
     2341 tgaggctgac gccctcccgc aggcgctggg tgcggaacag gtgggtcccc aggtcccggt
     2401 cgcccaggtt gaaccaggtc accccgccat agcggctcag ggcgtccatg gcctgccagg
     2461 tctcgccgac caggccccaa ccggtctcgg ggttcacggc cccggccagg gtgtaggtga
     2521 tggtgtccag gtcggggctg atgtggagcc catggagctc gacgtcgtct cccgtgttga
     2581 ccacggcggt gatcgaggcc gggtccacga cttggatcag cccgcccagc agccgggccg
     2641 ccccgacccc gccggcgacc accgcgacgg tcacgagatg ccgcgcagct tcccgttcag
     2701 caggtccaga tcggcactca ccatcatcgt caccaggtcc tcgaagtcca cggtgggggt
     2761 ccagccgagg acgctacggg ccttggccga gtccccgacc agcaggtcca cctcggcggg
     2821 tcgcatgaag gcttcgtcca tgatcacgta gtgctcccag tccaggtcga cgtggtcgaa
     2881 cgcgacctcg cagaactccc gcaccgaatg ggtctcgttg gtgctgacga cgtagttgtc
     2941 gggctcgtcc tgctgcagca tctgccacat ggccttgacg tagtccccgg cgtagcccca
     3001 gtcccgctgc gcctcgaggt tccccaagcg cagctcctcg gcgtgcccca gcttgatctg
     3061 ggccaccgtg tggctgatct tcctggtcac gaactccatc ccccgacggg gcgactcatg
     3121 gttgaacaga atgccgctgg atgcatggag gttgtacgac tcgcggtagt tgatcgtgat
     3181 ccagtggccg tagaccttgg ccacgccgta ggggctgcgg gggtagaacg gggtcgactc
     3241 ggtctggggg acctcctgga ccttgccgaa catctcgctg ctggacgcct ggtagaagcg
     3301 gatgtcggga tcgacgatgc ggatggcgtc gagcaggcgg gtgacgccga gggccgtggt
     3361 ctccccggtc aggaccggct ggttgaacga ggtctgcacg aaggactggg cggccaggtt
     3421 gtacacctcg tcgggccggt gggtggagag gatctcgatc atggagatct cgtcgagcac
     3481 gtcaccgggc acgatggtga tgcggtcctg gatgtgggcg atccgctcga agttcacggt
     3541 gctggaccgc cggaccatgc cgaccacctc gtagccgtgg tcgaggaggt gctcggccag
     3601 gtacgaaccg tcctggccgg tgatgccggt gatgagcgcg cgtcggggca tgggccccgt
     3661 tgtatcccgg gggcgggacc gtgtctgcca actggcttgg cagcgggccg accccgcgtt
     3721 cccgtacctt gaccccatgg cccgcgaact ggagcgcaac gtgctgggcc gccgc
//
LOCUS       DASTFV010000389         3664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1420497, whole genome shotgun sequence.
ACCESSION   DASTFV010000389 DASTFV010000000
VERSION     DASTFV010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420497"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VFP02_07155"
     CDS             complement(<1..1146)
                     /locus_tag="VFP02_07155"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9542842.1"
                     /translation="MALALHRLGRWCVTHRRRVLLLWLATLIGLGVFAGRVGGEYTDT
                     FRVPGVESQQATDLLQERFPTASGTSAQLVFHADDGGVLDGGRATVVAAVIAEVGELR
                     DVESVTDPTTQLSPDGSTALAQVQYGVEFTDLDVTDLDALLAVGEPLADQGLQMEVGG
                     ELPQFVEQPETQGAELIGLLAAVLILLLAFGSILAMGLPIGIALFGLGISVMLLTIVA
                     AVVDVPTTTPILASMIGIGVGIDYALFVVTRHREHLHDGMTVTEAAARAIATAGQAVI
                     IAGGTVVIAILGLAISGIPMVAVMGMGAAVVVAVMVIATITLLPALLGFADLRLTRAS
                     LPWTRRREAVDDEWHERLTRLGASPDLRPNRWQRWGDHVTSHPWRWLV"
     gene            1223..1843
                     /locus_tag="VFP02_07160"
     CDS             1223..1843
                     /locus_tag="VFP02_07160"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET9542843.1"
                     /translation="MGHTDRGVTAMHNLRERKKADTREAIRGAAVELFCTQGFDATTM
                     DAVAEHANVSVRTVFRYFPTKEDLVFADVEEDLADLRELLDARPATEPVMDGVRAVVE
                     VLAGRMETGEDEDVRLAPLLHDEPALRQRHLAVLDRVEETVADWARTRLDAGPADLRP
                     GLLAACMVSVQRVVVDAVVAGDPRPLSDLIREAVAIIGRGFDRLDP"
     gene            complement(1840..>3664)
                     /locus_tag="VFP02_07165"
     CDS             complement(1840..>3664)
                     /locus_tag="VFP02_07165"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET9542844.1"
                     /translation="PSFPRDDVPADGPYPTVVNMSGYSPADPSSPPPELQLADALGYA
                     TVGVNLRGTGCSGGALSFFEGPQVADGYDAIEAIAAQDWVAGNEVGMVGISYPGITQL
                     FVASTQPPHLAAISPMSVIDDPYVGVGYPGGIFNSGFAAEWTSHVSDDAAAYGPDYVN
                     QQIDDGDTDCDGNQALRSQNLDLVAGLEAEPFDDADRFGPIRPAELVDQIDVPVFLTG
                     QWQDEQTSGHFADMLDRFTGTDRLHAYLTNGPHGDGLTLPNFQRWADFLDLYVAHRIP
                     ELPAVARAGVPPAINAIFGEGVGLGPDRFAGYDSYEEALADWEADDPFTVVFENGAAT
                     DHPGGPGGTTEATFASWPPPEAEATTWYFEPDGTLGPDEPTVADGEDGSVVEYAQVPG
                     QARRRTLVEQSDDISFSPQPPYRWPSPQPGEVATWLTPALEDDLAVVGSARADVYLQS
                     SAPDTDLEVTLTEVTPDGDEVYVQTGWLRASHRALDEERSTPIHPVPLHTEAAAEPLP
                     DGEFVEVSVDIFPFAHLFRAGSRLRLSIDSPGGNRARWAFDTVDAASESNLIADSADF
                     PSSLTLAVVDGVQVPRALPACGTLRAQPCRPYQPVSNVPAG"
BASE COUNT          535 a         1222 c         1421 g          486 t
ORIGIN      
        1 caccagccag cgccacgggt gcgacgtgac gtggtcgccc cagcgctgcc accggttcgg
       61 ccgcaggtcg ggggacgctc cgaggcgggt gaggcgctcg tgccactcgt cgtcgacggc
      121 ctcgcggcgg cgggtccacg gcaggctggc ccgcgtcagc cgcaggtcgg cgaagccgag
      181 cagggcgggc agcagggtga tggtggcgat gaccatcacc gccaccacga cggcggcccc
      241 catgcccatg acggccacca tggggatgcc cgagatggcc aagccgagga tggcgatcac
      301 cacggtgccg ccggcgatga tcacggcctg accggcggtg gcgatggccc gggccgcggc
      361 ctcggtcacc gtcatgccgt catggaggtg ctctcggtga cgggtgacga cgaacagggc
      421 gtagtcgatc cccaccccga tcccgatcat cgaggccagg atcggcgtcg tggtcgggac
      481 gtcgaccacc gcggcgacga tggtgagcag catcacgctg atccccagcc cgaagagggc
      541 gatgccgatg ggcagaccca tggccaggat ggagccgaag gcgagcagga ggatgagcac
      601 agcagccagc aggccgatca gctcggcccc ctgggtctcg ggctgctcca cgaactgagg
      661 cagctcaccg cccacctcca tctggaggcc ctggtcggcc aacggctcac cgacggcgag
      721 cagggcgtcg aggtcggtga cgtcgaggtc ggtgaactcg acgccgtatt gcacctgggc
      781 cagggccgtg ctgccgtccg ggctcagctg ggtcgtgggg tcggtcaccg actcgacgtc
      841 gcgcagctcg cccacctcgg cgatgaccgc ggcgaccacg gttgctcgcc cgccgtcgag
      901 gacgccgccg tcgtcggcgt ggaacaccag ctgggccgac gtgcccgacg ccgtggggaa
      961 gcgctcttgc aggaggtccg tggcctgctg ggactcgacc ccggggacac ggaaggtgtc
     1021 ggtgtactca ccgccgaccc ggccggcgaa gacaccgagg ccgatcaggg tggcgagcca
     1081 gaggagaagg acccggcggc ggtgggtgac gcaccagcgg ccgagacggt ggagggcgag
     1141 ggccatccgc ctagtgtgac agttactgtc aaaagtcact aggtgacata ggtcacccga
     1201 ccggctcggc cggcccccct cggtgggcca cactgaccgg ggggtcacgg cgatgcacaa
     1261 cctgcgcgaa cgcaagaagg cggacacccg ggaggcgatc cgcggggccg cggtcgagct
     1321 gttctgcacg cagggcttcg acgccacgac gatggacgcc gtcgccgagc acgccaacgt
     1381 ctcggtccgc accgtcttcc gctacttccc caccaaggag gacctggtct tcgccgacgt
     1441 cgaggaggac ctggccgacc tccgggagct gctggacgcc cggccggcca ccgagccggt
     1501 gatggacggc gtccgggccg tcgtcgaggt gctggccggt cggatggaga cgggagagga
     1561 cgaggacgtg cggctggccc ctctgctcca cgacgagccc gccctgcgcc agcgccatct
     1621 cgcggtgctc gaccgggtgg aggagacggt ggccgactgg gccaggaccc gcctcgacgc
     1681 cgggccggct gatctccgcc ccgggctgct cgcggcctgc atggtctcgg tccagcgcgt
     1741 cgtcgtcgac gccgtcgtgg ccggtgaccc ccgtcccctc agcgacctca tccgcgaggc
     1801 cgtcgccatc atcggccgcg gcttcgaccg gctcgacccc tagccggcgg ggacgttgct
     1861 cacgggctgg taggggcggc agggctgggc ccggagcgtc ccgcaggcgg gaagggcgcg
     1921 aggcacctgc acgccgtcga ccacggccaa ggtgagcgac gacgggaagt cagccgaatc
     1981 agcgatgagg ttggactcac tggcggcatc gaccgtgtcg aaggcccagc gggcccggtt
     2041 gccgccgggg ctgtcgatgg acagccggag ccgggacccg gcccggaaga ggtgggcgaa
     2101 cgggaagatg tccaccgaga cctcgacgaa ctccccgtcg ggcagcggct cggccgcggc
     2161 ctcggtgtgc aggggcacgg ggtggatggg cgtggaccgc tcctcgtcca gggcccgatg
     2221 gctggcccgg agccaacccg tctggacgta gacctcgtcg ccgtcggggg tgacctcggt
     2281 caaggtgacc tcgagatcgg tgtcgggcgc actggactgc aggtacacgt cggcccgggc
     2341 cgagccgacg acggcgagat cgtcctccag ggccggggtc agccacgtgg ccacctcacc
     2401 cggctgcggc gagggccacc ggtagggagg ttggggcgag aacgagatgt cgtcggactg
     2461 ctccacgagg gtccgccggc gggcctgccc cgggacctgg gcgtactcca cgaccgaccc
     2521 gtcctcaccg tcggccaccg tgggctcgtc ggggccgagg gtcccgtccg gctcgaagta
     2581 ccaggtggtg gcctcggcct cggggggcgg ccaggaggcg aacgtggcct ccgtcgtccc
     2641 gcccggcccg ccggggtggt cggtggcggc cccgttctcg aacaccacgg tgaaggggtc
     2701 gtcggcctcc cagtcggcca gggcctcctc gtaggagtcg tagccggcga agcggtcggg
     2761 cccgaggccc acgccctcgc cgaagatggc attgatggcc ggcggcaccc cggctcgggc
     2821 gacggcgggc agctcgggga tgcgatgggc cacgtacagg tccaggaagt cggcccagcg
     2881 ctggaagttg ggcagggtca ggccgtcgcc gtgggggccg ttggtcaggt aggcgtgcag
     2941 ccggtcggtg ccggtgaagc ggtcgagcat gtcggcgaag tggccactgg tctgctcgtc
     3001 ctgccactgg ccggtgagga acacggggac gtcgatctgg tcgacgagct cggcgggccg
     3061 gatcgggccg aaccggtcgg catcgtcgaa gggctcggct tcgaggccgg ccaccaggtc
     3121 caggttctgg gagcgcaggg cctggttgcc gtcgcagtcg gtgtccccgt cgtcgatctg
     3181 ctggttgacg tagtcgggcc cgtaggcggc ggcgtcgtcg ctgacgtggc tggtccactc
     3241 ggccgcgaac ccgctgttga agatgccgcc ggggtagccg acgccgacgt agggatcgtc
     3301 gatgaccgac atcgggctga tggccgcgag gtgggggggc tgggtcgacg ccacgaagag
     3361 ctgggtgatg ccgggatagg agatgccgac catcccgacc tcgttgccgg cgacccagtc
     3421 ctgggcggcg atggcctcga tggcgtcgta gccgtcggcc acctgtgggc cttcgaagaa
     3481 cgagagggcc ccgcccgaac agccggtgcc ccgcaggttc accccgacgg tggcgtagcc
     3541 gagggcatcg gccagctgca gctcgggggg tgggctggag gggtcggcgg gggagtagcc
     3601 cgacatgttg accacggtgg ggtagggccc gtcggccggg acgtcgtccc gcgggaagga
     3661 cggg
//
LOCUS       DASTFV010000390         3180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1426367, whole genome shotgun sequence.
ACCESSION   DASTFV010000390 DASTFV010000000
VERSION     DASTFV010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426367"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /locus_tag="VFP02_07170"
     CDS             <1..1450
                     /locus_tag="VFP02_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HET9542845.1"
                     /translation="RSRQLAHLLRERGLGPGSHVAVLLENHVRYFEVCWAAQRSGLYI
                     TPINWHLGGEEAGYILSDCEATALVTSSRMSDLVASLGPHLDGVTTRLSVDGGVEGFE
                     HYETVLAPQPTEPLDDETEGSLMLYSSGTTGHPKGILPPLSGAPFGTPGVVDALVGMY
                     GFGVGTRYLVPAPLYHAAPLGWSMGAQRAGGTVVVLERFDPTVALAAIEEHRVTHAQF
                     VPTHFVRMLKLDEAARTAHDLSSLQMAIHAAAPCPVEVKRQMLEWWGPIIHEYYSGSE
                     GVGFCAVGPDEWLEHPGTVGKALMGVPHIVGEDGEEVPSGEVGQIWFEGAKPFEYHGD
                     PEKTASVFDQNGWATLGDVGYLDDDGYLFLTDRVSHMIISGGVNIYPQEIEDALIVHP
                     AVADAAVIGVPDADMGESVMAVIQPADPDADEAALTAELQGYCRERLAGFKCPRSYRF
                     TTALPRLPTGKLLKRKLRDEMGGSSAAGVIGG"
     gene            complement(1447..2403)
                     /locus_tag="VFP02_07175"
     CDS             complement(1447..2403)
                     /locus_tag="VFP02_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9542846.1"
                     /translation="MPTKTVLVLGGGGAVGVAYHCGALRALEEITGLDPRDADLLIGT
                     SSGAIMATEFALGRTVDDIAAEVHPEEPRERGLERAWRSPVDLARRTVGASYLVARIA
                     LARGWPLPEPPRWMQRALPGALFSVGDGDWGPIHYPEKWPDQALWLVTADLDGGGRRV
                     VLDRDGVSGLPASLGTAVMASCAVPGVLPPVRVGGRRLIDGGLRSSTNLDLARGVDAD
                     VVVALAPMAFASDPGPSPLHMVGRRRFNARLEKESEAVRATGASVLVLRPGADELRLH
                     SSNILSRDNGQAIIAAAYDATMARRDEIAALVRVDGARNRSA"
     gene            2525..>3180
                     /locus_tag="VFP02_07180"
     CDS             2525..>3180
                     /locus_tag="VFP02_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542847.1"
                     /translation="MASDLDYDPYDREIDADPYPTYRRLRDEAPLYRNEAYDFYAVSR
                     FDDVQRGLVDRETYSSARGAVLEFIQADIEIPPGVVIFEDPPQHTVHRGLLSRVFTPK
                     KMEALEPKVREFCARALDPLVGVGGFDFVADLGAQMPMRTIGMLLGVPEADQEAARDR
                     ADASLRTDPGQPMAAEQMEIGQGDFFADYLDWRTANPSDDLMTELLFAEFEDETGTTR
                     R"
BASE COUNT          469 a         1178 c         1097 g          436 t
ORIGIN      
        1 ccgcagccga cagctggccc acctgctccg ggaacggggc ctgggcccgg gcagccacgt
       61 ggccgtgctg ctcgagaacc acgtccggta cttcgaggtg tgttgggcgg cgcagcggtc
      121 gggcctgtac atcacaccga tcaactggca cctcggcggg gaggaggccg gctacatcct
      181 ctcggactgc gaggccaccg cgctggtgac ctcctcccgc atgagcgacc tcgtcgcgtc
      241 cctgggacca cacctcgacg gggtgaccac ccgactgagc gtcgacgggg gggtcgaggg
      301 cttcgagcac tacgagacgg tgctggcgcc ccaacccacc gagcccctgg atgacgagac
      361 cgagggctcg ctgatgctct actcgtcggg cacgaccggt caccccaagg gcatcctccc
      421 cccgctcagc ggcgccccgt tcggcacgcc tggcgtcgtc gacgcgctgg tcggcatgta
      481 cggcttcggc gtcgggaccc ggtacctcgt gccggcgccg ctgtaccacg ccgccccgct
      541 cggctggtcc atgggcgccc agcgagcggg cggcacggtc gtggtgctgg agcgcttcga
      601 cccgacggtc gccttggccg ccatcgagga gcaccgcgtc acccacgccc agttcgtccc
      661 cactcacttc gtgcggatgc tgaagctcga cgaggcggcc cggaccgccc atgacctgtc
      721 gagcctccag atggcgatcc acgcggccgc cccctgcccg gtcgaggtca agcgccagat
      781 gctcgagtgg tggggcccga tcatccacga gtactactcg ggcagcgagg gcgtcggctt
      841 ctgcgccgtc ggccccgacg agtggctgga gcacccgggc acggtgggca aggcgctgat
      901 gggcgtgccc cacatcgtcg gggaggacgg cgaggaggtc ccctccggcg aggtcggcca
      961 gatctggttc gagggggcga agccgttcga gtaccacggc gatcccgaga agacggcgtc
     1021 ggtcttcgac cagaacggct gggccacgct gggcgacgtc ggctacctcg acgacgacgg
     1081 ctacctcttc ctcaccgacc gcgtgtcgca catgatcatc tccggaggcg tgaacatcta
     1141 tccccaggag atcgaggacg ccctcatcgt ccaccccgcc gtggccgacg ccgcggtcat
     1201 cggcgtgccc gacgccgaca tgggtgaatc ggtgatggcg gtcatccagc ccgccgaccc
     1261 cgacgccgac gaggccgccc tcaccgcgga gctccagggg tactgccgtg agcgactggc
     1321 cggcttcaag tgcccgcggt cctaccggtt caccaccgcg ctcccccgcc tccccaccgg
     1381 caagctgctc aagcgcaagc tccgggacga gatgggtggc tcctccgccg ccggcgtgat
     1441 cggcggttag gcgctccggt tcctggcgcc gtcgacccgc acgagggcgg cgatctcgtc
     1501 ccggcgggcc atggtggcgt cgtaggccgc ggcgatgatc gcctgaccgt tgtcgcggct
     1561 caggatgttg gaggagtgga ggcggagctc gtcggccccc ggtcgcaaca cgaggacgct
     1621 ggctccggtc gcccgcacgg cctcggactc cttctccagc cgggcgttga accgccggcg
     1681 accgaccatg tggagcgggc tggggccagg gtcggatgcg aaggccatcg gggccagggc
     1741 gacgacgacg tcggcgtcga ccccccgggc caggtcgagg ttggtggacg accggaggcc
     1801 cccgtcgatc agccgccggc cgccgacccg tacgggcggc agcacgccgg gcaccgcgca
     1861 gctcgccatc accgccgtcc ccagcgacgc cggtaggccg gagacgccgt cgcggtcgag
     1921 gaccacccgc cgaccaccac cgtcgagatc ggccgtgacc agccacagcg cctggtccgg
     1981 ccatttctcc gggtagtgga tcgggcccca gtccccgtcg cccaccgaga acagggcccc
     2041 gggcagggcg cgctgcatcc aacggggcgg ctcgggcagg ggccaccccc gagccagcgc
     2101 aatacgggcg accaggtagc tcgcgcccac cgtccggcgg gcgagatcca ccggcgaccg
     2161 ccaggcccgc tcgagcccgc gctctctcgg ctcctcggga tggacctcgg cggcgatgtc
     2221 gtcgacggtc cgaccgaggg cgaactcggt ggccatgatc gccccgctgc tggtgccgat
     2281 caggaggtcg gcgtcgcggg ggtccagccc cgtgatctcc tccagcgccc gcagcgcccc
     2341 acagtggtag gcgacgccca cagcaccgcc cccgccgagg acgaggaccg tcttggtcgg
     2401 cacccgggca gggtagcccc cggcggcaac gtcatgagca ttgacggtga agaacagtct
     2461 gcttcccgag aactccgttc tcgcttgtcg gctcctagcc gaacggctag cttcggcgac
     2521 ggccatggcg tccgacctcg actacgaccc gtacgaccgt gagatcgacg ccgatcccta
     2581 cccgacctac cgccggctgc gcgacgaggc gcccctctac cgcaacgagg cgtacgactt
     2641 ctacgccgtg agtcggttcg acgacgtcca acggggactc gtcgaccgcg agacctacag
     2701 ctcggcgcgc ggcgccgtgc tggagttcat ccaggcggac atcgagatcc cgccgggcgt
     2761 cgtcatcttc gaggacccgc cgcagcacac cgtccaccgg ggcctgctct cgcgggtgtt
     2821 caccccgaag aagatggagg cgctggagcc caaggtgcgg gagttctgcg cccgcgccct
     2881 cgacccgctg gtcggcgtcg ggggcttcga cttcgtcgcc gacctcggcg cgcagatgcc
     2941 gatgcggacc atcgggatgc tcctcggtgt ccccgaagcc gaccaggagg cggctcgcga
     3001 ccgagccgat gcgtcgctgc gcaccgaccc gggccagccg atggccgccg agcagatgga
     3061 gatcgggcag ggcgacttct tcgccgacta cctcgactgg cggacggcga acccctccga
     3121 cgacctcatg accgagttgc tgttcgccga gttcgaagac gagaccggga cgacccgccg
//
LOCUS       DASTFV010000391         2395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1428712, whole genome shotgun sequence.
ACCESSION   DASTFV010000391 DASTFV010000000
VERSION     DASTFV010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428712"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VFP02_07185"
     CDS             complement(<1..875)
                     /locus_tag="VFP02_07185"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9542848.1"
                     /translation="MDLILRNGTVIDGTGAPRRSADVGVADGRVVAVGEVAATDGAEE
                     VDLGGLVLAPGFVDIHTHYDAQVLWDPDLTPSSWHGVTSVVVGNCGFGIAPTRPEHRE
                     TIARTLENVEGMAFDALVAGIDWTFESFPEYLATIDRLPKRLNVGVLAGHTPTRLYVL
                     GDEASDREATEAEVAEMAAIIKEALEAGAVGFSTSGAPTHAGAYGKPVPSRLATYDEI
                     KALAATMGEVGHGVMQATVGANLFMDQFAEISQLTGRPVSWTALLSGMWGEGGAVSII
                     DATAQRGGDVWPQVA"
     gene            complement(929..1756)
                     /locus_tag="VFP02_07190"
     CDS             complement(929..1756)
                     /locus_tag="VFP02_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542849.1"
                     /translation="MSRAFARAAAVGLLLLAACGDDGGSQVGGGATGVELTATATRSS
                     LFDSQRTFRLELTNDGDVSVTVASVQLDSPLFEPVPASSRDTQLAPGQRLLLPVPYGP
                     SICPGAGPSNLVTVVSGDEVVVPLGQVPVGVMDALHEVECGEAAVRDAADVDFGADWT
                     STGPRSARGDIEVETHGETEVAVDGVRGNIVFGVRVADTDPPLLTVARAGEVGRLPLE
                     LVVDRCDTHALIESKRTFKFPLEVGLDGGPPALVVLEAEPGSPTRDVLGGLIQACIG"
     gene            complement(1753..>2395)
                     /locus_tag="VFP02_07195"
     CDS             complement(1753..>2395)
                     /locus_tag="VFP02_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9542850.1"
                     /translation="GHARCGLVAGDVGAVVAYDLALRFEGLVEGLCYFNTAPPFLVEE
                     YQAAGISLTGFGDVDARPETDYQLRQGQHADALAADLDTPERRRRYIADFYGHRLWGS
                     RGSFTEADVDFMTEPWSEEGRLRAGWAVYQLARGTRVAEEVPRLFETMPVPSLLLYGP
                     EDQVVPEEFTACCEVAFTNRTGPLVVPRAGHFLQWERADVLNALVAGWFSQFR"
BASE COUNT          346 a          878 c          855 g          316 t
ORIGIN      
        1 caggccacct gtggccacac gtccccgccc cgctgggccg tggcgtcgat gatcgagacg
       61 gctccgccct cgccccacat ccccgagagc aacgccgtcc aggagaccgg tcgcccggtg
      121 agctgggaga tctcggcgaa ctggtccatg aacaggttgg cccccaccgt ggcctgcatc
      181 accccgtggc cgacctcgcc catcgtcgcg gcgagggcct tgatctcgtc gtaggtggcc
      241 aggcggctgg ggacgggctt gccgtaggcc ccggcgtggg tgggagcccc ggacgtggag
      301 aaccccaccg cccccgcctc gagggcctcc ttgatgatgg cggccatctc ggccacctcg
      361 gcctcggtcg cctcccggtc cgacgcctcg tcgcccagga cgtagaggcg ggtcggggtg
      421 tgcccggcga ggacgccgac gttcagccgc ttggggaggc ggtcgatcgt ggccaggtac
      481 tcgggaaagg actcgaaggt ccagtcgatg ccggccacga gggcgtcgaa cgccatgccc
      541 tcgacgttct ccagcgtgcg ggcgatggtc tcgcggtgct ccggtcgggt cggggcgatg
      601 ccgaacccgc agttgcccac gaccacgctg gtcacgccgt gccagctcga cggcgtcagg
      661 tccgggtccc acaggacctg ggcgtcgtag tgggtgtgga tgtcgacgaa cccgggggcc
      721 aggaccagtc cgccgaggtc gacctcctcc gccccgtcgg tggcggcgac ctcgccgacg
      781 gccaccaccc ggccgtcggc cacgccgaca tcggcggacc ggcgcggtgc cccggtcccg
      841 tcgatgaccg tcccgttgcg caggatcagg tccatgtgtc ccccttcagg tccccgtcga
      901 ggcctcacgg cccggcgcga ccgtaccgtc agccgatgca ggcctggatc aggccgccca
      961 ggacgtcccg ggtcgggctg ccgggctcgg cctccagcac gacgagcgcc ggtgggccac
     1021 cgtccagacc tacctccagc gggaacttga acgtgcgctt gctctcgatg agggcgtggg
     1081 tgtcgcaacg gtcgacgacg agctcgagcg gcagccggcc gacctcgcct gcccgggcca
     1141 cggtgaggag gggcggatcg gtgtcggcca cgcgcacgcc gaagacgatg ttgccccgca
     1201 cgccgtcgac ggccacctcg gtctcgccgt gcgtctcgac ctcgatgtcc ccccgggccg
     1261 accggggacc ggtcgacgtc cagtcagcgc cgaagtccac gtcagcggcg tcccgcaccg
     1321 cagcttcgcc gcactccacc tcgtggaggg cgtccatgac ccccaccggg acctggccca
     1381 gtgggaccac gacctcgtca ccggacacga ccgtcaccag gttcgacggg cccgcgccgg
     1441 gacagatcga cgggccgtag ggcacaggaa gcaggagccg ctggcccggg gccagctggg
     1501 tgtcgcggga gctggccggc acgggctcga acagcggtga gtcgagctgg accgacgcca
     1561 ccgtgacgga gacgtcgccg tcgttggtca gctcgagacg gaaggtgcgt tggctgtcga
     1621 acaggctgga gcgggtggcg gtcgccgtca gctcgactcc ggtcgccccg cccccgacct
     1681 ggcttccgcc gtcgtcacca cacgccgcca gcagcagcaa cccgaccgcg gcggcccgag
     1741 cgaacgcccg gctcaccgga actgggagaa ccagcccgcg accaaggcgt tgagcacgtc
     1801 ggcccgctcc cactgcagga agtggccggc ccggggcacg accagcggtc cggtccggtt
     1861 cgtgaacgcc acctcgcagc aggcggtgaa ctcctcgggg acgacctggt cctcggggcc
     1921 gtagaggagc agggagggca cgggcatcgt ctcgaagaga cggggcacct cctcggccac
     1981 ccgggtcccc cgggcgagct gatagacggc ccagcccgcc cggagccgac cctcttccga
     2041 ccagggctcg gtcatgaagt cgacgtcggc ttccgtgaac gagccccggc tgccccagag
     2101 ccggtggccg tagaaatcgg cgatgtaacg ccggcgccgc tcgggtgtgt ccaggtccgc
     2161 ggccagggca tcggcgtgct ggccctggcg cagctggtag tcggtttccg ggcgggcgtc
     2221 gacgtcgccg aagcctgtca gggagatgcc cgcggcctgg tactcctcga ccaggaacgg
     2281 cggggcggtg ttgaagtagc acaggccttc gaccaggccc tcgaagcgca gggccaggtc
     2341 gtaggccacg accgcgccga cgtcaccggc caccaatcca cagcgcgcgt ggccg
//
LOCUS       DASTFV010000392         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1439642, whole genome shotgun sequence.
ACCESSION   DASTFV010000392 DASTFV010000000
VERSION     DASTFV010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439642"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VFP02_07200"
     CDS             complement(<1..621)
                     /locus_tag="VFP02_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HET9542851.1"
                     /translation="MSTTDRPVTPPAPRQPAAVEIYDTTLRDGSQLEGISLTVDDKLR
                     IAEQLDWLGVHYIEGGWPGANPKDDEFFRRAVTELELTTSELVAFGSTRRVKGKVDAD
                     ETLANLLGAGTGTVCIVGKCWDYHVTEALRTDLDEGVAMVHDSVAYLVGQDRRVFFDA
                     EHFFDGYKANPEFSLRVLEAAAMAGADCLVLCDTNGGSLPFDVERIV"
     gene            complement(608..1528)
                     /locus_tag="VFP02_07205"
     CDS             complement(608..1528)
                     /locus_tag="VFP02_07205"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET9542852.1"
                     /translation="MPIETVEKIWMDGELVAWEDAKIHVLTHTLHYGCGVFEGIRAYE
                     TSRGPAVFRLDDHIDRLFNSAAIYMIDVPFDRDQLIAATLETVRVNEVRSCYIRPLVY
                     LGYGEMGLNPLPCPVNVAIAIWPWGTYLGDEGIQNGIRLKISSWRRHDPNTMPPAAKG
                     TGMYINSSMAKVEALKAGYDEAVMLSPQGNVSECTGENIFVVNDGAIVTPPTSAGALE
                     GITQDSVRRIAEGLGVDYRVENLLRSDLYTAEEAFLSGTAAEVVPIRSVDDRELGDPG
                     PITRQIQETYFAAVRGEDDRYKDWLSHVDD"
     gene            complement(1530..>2194)
                     /locus_tag="VFP02_07210"
     CDS             complement(1530..>2194)
                     /locus_tag="VFP02_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET9542853.1"
                     /translation="PTIDFLVVRENTEGAYAGEGGFLRKDTPHEIATQGSVNTRMGVE
                     RAVRFAFDLARSRERRHLTLVHKTNVLTFAGDLWERAFHAVAAEYDDVTTAYHHVDAA
                     CIHFVQDPGRYDVIVTDNLFGDILTDLGGAVAGGIGRAASANLNPARTGPSMFEPVHG
                     SAPDIAGTGTADPTAAVLSAAMMLEFLGEADAAARIQKACAEPAPEGSTADIGDAIAE
                     RV"
BASE COUNT          312 a          718 c          795 g          369 t
ORIGIN      
        1 cacgatgcgc tcgacgtcga agggcaggga gccgccgttg gtgtcgcaga ggacgaggca
       61 gtcggccccg gccatggccg cggcttcgag gacccggagg ctgaactccg ggttggcctt
      121 gtagccgtcg aagaagtgct cagcgtcgaa gaacacccgt cggtcctggc ccaccaggta
      181 cgcgaccgaa tcgtggacca tggccacgcc ctcgtcgagg tcggtgcgga gggcttcggt
      241 gacgtggtag tcccagcact tcccgacgat gcacacggtc ccggttcccg ctcccagcag
      301 gttggccaag gtctcgtcgg cgtcgacctt gcccttgacc cgtcgggtcg aaccgaacgc
      361 caccagctcg ctggtcgtca gctccagctc ggtgacggcc cggcggaaga actcgtcgtc
      421 cttcgggttg gccccgggcc agcccccttc gatgtagtgg acaccgagcc agtcgagctg
      481 ttcggctatc cgcagcttgt cgtcgacggt cagggagatg ccctcgagct ggctgccgtc
      541 gcgcagggtc gtgtcgtaga tctcgaccgc ggcgggctgg cggggcgcgg gtggggtgac
      601 ggggcggtca gtcgtcgaca tggctgagcc agtccttgta gcggtcgtcc tcgccccgga
      661 ccgcggcgaa gtaggtctcc tggatctgac gggtgatcgg gccgggatcg cccagctcgc
      721 ggtcgtcgac cgagcgaatg gggacgacct cggccgccgt gccgctgagg aaggcctcct
      781 cggccgtgta gaggtcgctc cgcagcaggt tctcgacgcg gtagtcgacc ccgaggccct
      841 cggcgatgcg gcgcaccgag tcctgcgtga tgccttcgag agcacccgcc gaggtcggcg
      901 gcgtgacgat cgcgccgtcg ttgacgacga agatgttctc gccggtgcac tcgctgacgt
      961 tgccctgcgg cgacagcatg acggcctcgt cgtagcccgc cttcagggcc tcgaccttcg
     1021 ccatcgacga gttgatgtac atgccggtgc ccttggcggc ggggggcatg gtgttcgggt
     1081 cgtggcgccg ccaggacgag atcttcaggc ggatcccgtt ctggatcccc tcgtcgccga
     1141 ggtaggtccc ccagggccag atggcgatgg ccacgttcac cgggcacggc agcgggttga
     1201 ggcccatctc gccgtacccg aggtacacca gcgggcggat gtagcaggag cggacctcgt
     1261 tgacccggac cgtctccagc gtcgccgcga tcagctggtc ccggtcgaac gggacgtcga
     1321 tcatgtagat ggcggccgag ttgaagaggc ggtcgatgtg gtcgtcgagg cggaacacgg
     1381 ccgggccccg ggaggtctcg taggcgcgga tgccttcgaa gacgccgcag ccgtagtgca
     1441 gcgtgtgggt caggacatgg atcttggcgt cctcccaggc gaccagctcg ccgtccatcc
     1501 agatcttctc aacggtctcg atcggcatct cagactcttt ctgcgatggc atcgccgatg
     1561 tcggcggtgc tcccctcggg cgccggttcg gcgcaggcct tctggatccg ggcggcggca
     1621 tcggcctccc ccaggaactc gagcatcatc gccgcggaaa ggaccgctgc ggtgggatcc
     1681 gccgtcccgg tccccgcgat gtcgggggcc gagccgtgca cgggctcgaa catggagggg
     1741 cctgtccggg cggggttcag gttggccgaa gcggcccgcc cgatgccccc ggcgaccgcg
     1801 cccccgagat cggtgaggat gtcgccgaac aggttgtcgg tgacgatcac gtcgtaccgg
     1861 ccgggatcct gcacgaagtg gatgcaggcg gcgtcgacgt ggtggtaggc cgtggtgacg
     1921 tcgtcgtact cggcggccac cgcgtggaac gcccgctccc agaggtcacc ggcgaaggtc
     1981 agcacattgg tcttgtggac cagagtgaga tgccggcgct cccggctgcg ggccaggtcg
     2041 aaggcgaacc ggacggcgcg ctcgaccccc atccgggtgt tgaccgatcc ctgggtcgcg
     2101 atctcgtggg gcgtgtcctt gcgcaggaag ccgccctcgc cggcgtaggc cccctcggtg
     2161 ttctcgcgca cgacgaggaa gtcgatcgtc ggcg
//
LOCUS       DASTFV010000393         2881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1440236, whole genome shotgun sequence.
ACCESSION   DASTFV010000393 DASTFV010000000
VERSION     DASTFV010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440236"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VFP02_07215"
     CDS             <1..825
                     /locus_tag="VFP02_07215"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HET9542854.1"
                     /translation="PIRIGVNGGSLDPDLYEKHGGLTAEAMVESAQRELDYFAEVGFD
                     DVKISVKASNVPLMIEAYRQLSEATDYPLHLGVTEAGPPPAGLIKATAGLATLLSEGI
                     GDTIRYSLTADPVQEARAGRQLLESLGLRERTGLDLIACPSCGRAEVDVYAIADAAQK
                     ALEELEVPIQVAVMGCVVNGPGEAREADLGIAAGRKRGHLFVKGKVVKVVAEDEMVEA
                     LVEWAKIVSEDGVEAALARAESGAAEEAEKDRAALLTEQGDDVNDAEAHIDQIRKL"
     gene            complement(842..1267)
                     /locus_tag="VFP02_07220"
     CDS             complement(842..1267)
                     /locus_tag="VFP02_07220"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9542855.1"
                     /translation="MSDEQPTTRRPVVLAPGGGRTYDMGRIQATFKADGDETAGAYSI
                     SEWRLEANTSGPGPHHHDEDDVFFVTEGTMSVLVDDEWIDAPTGAFVVVPGGTTHDFE
                     NRSHAPAAVLNVSAPGNFEAHMADIVSWFAEHPAGDAVP"
     gene            1359..2771
                     /gene="proS"
                     /locus_tag="VFP02_07225"
     CDS             1359..2771
                     /gene="proS"
                     /locus_tag="VFP02_07225"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET9542856.1"
                     /translation="MAKAPVLTPQSEDFPRWYQDVIAKAELAENGPARGTMVIRPYGF
                     SLWERLQAAMDDRIKAAGVDNAYFPLFVPMSFFEREAEHVEGFSPELAVVTIGGGKEL
                     EEPLAIRPTSETVMGTYFAKWIQSYRDLPLLVNQWANVVRWELRPRLFLRTTEFLWQE
                     GHTCHATEAEARDFAVRILHEVYADCMEQVLAVPVYKGRKTAAERFAGAINTMACEAM
                     MRDGKALQMGTSHELGQNFAKAFGIEFLSAEGNQENVWQTSWGTSTRMVGGLIMTHGD
                     DNGLRVPPVLAPIQVVVLVVRDEEGAGDAAARLADELKAAGVRVRLDDKVDTSFGRRA
                     TNWELKGIPLRIEVGPRDLKEGEVTLVRRDNGEKRQVAVTGVAGEVPGLLDTIQADLL
                     AGATKDRDDRTVDVSSIEDVAEAAANGFVRIPWDSVGLEGESRLANDALTVRCLQRAD
                     GSLPGADDEPDLIALVARSY"
BASE COUNT          441 a         1012 c         1022 g          406 t
ORIGIN      
        1 cccatccgca tcggggtcaa cggcggctcg ctcgaccccg acctctacga gaagcacggc
       61 ggcctgaccg ctgaggccat ggtcgagtcg gcgcagcggg agctggacta cttcgccgag
      121 gtcggcttcg acgacgtgaa gatctccgtg aaggcgtcga acgtcccgct gatgatcgag
      181 gcctaccgcc agctgtccga ggccaccgac tacccgttgc atctcggcgt gaccgaggcc
      241 ggcccgcctc ccgccggcct gatcaaggcc accgccggcc tggccacgct cctgtcggag
      301 ggcatcggcg acaccatccg gtactcgctg accgccgatc cggttcagga ggcccgggcc
      361 gggcgccagc tcttggagtc gctgggcctg cgggagcgca ccggcctgga cctgatcgcc
      421 tgcccatcgt gcggccgggc cgaggtcgac gtctacgcca tcgccgacgc ggcccagaag
      481 gccctcgagg agctcgaggt ccccatccag gtcgccgtca tgggctgcgt ggtgaacggg
      541 ccgggcgagg cccgggaggc cgacctcggc atcgccgccg gccgcaagcg cggccacctg
      601 ttcgtcaagg gcaaggtcgt caaggtcgtg gccgaggacg agatggtcga ggccctcgtc
      661 gagtgggcca agatcgtcag cgaggacggc gtcgaagccg ccctggcccg cgccgagtcg
      721 ggcgcggcgg aggaggccga gaaggaccgc gccgcgctcc tgaccgagca gggtgacgac
      781 gtcaacgacg ccgaagccca catcgaccag atccgcaagc tctgacggtc ggggcggtcg
      841 gttacgggac ggcgtcgccc gcggggtgct cggcgaacca gctgacgatg tccgccatgt
      901 gggcctcgaa gttgccggga gccgagacgt tcaggacggc ggcgggggcg tggctgcggt
      961 tctcgaagtc atgcgtggtc ccgccgggca cgaccacgaa cgccccggtc ggggcgtcga
     1021 tccactcgtc gtcgacgagg accgacatgg tcccctcggt cacgaagaag acgtcgtcct
     1081 cgtcgtggtg gtggggcccc ggcccgctcg tgttcgcctc gaggcgccac tccgagatcg
     1141 agtaggcgcc ggcggtctcg tcgccatcgg ccttgaacgt ggcctggatc cggcccatgt
     1201 cgtaggtccg gccgccgcca ggggcgagga cgaccgggcg cctcgtggtg ggctgctcgt
     1261 cgctcatcgg tcctgcctcc tcggcccgaa ggtagcggcg ggaccgctct tgcggaacgg
     1321 agccagctcg gcggggcctc gccactaccc tccgccggat ggccaaggct cccgtgctca
     1381 ccccccagag tgaggacttt ccccggtggt accaggacgt catcgccaag gccgagctgg
     1441 ccgagaacgg gccggctcgg gggacgatgg tcatccggcc ctacgggttc tccctctggg
     1501 agcgcctcca ggcggcgatg gatgaccgca tcaaggccgc cggggtcgac aacgcctact
     1561 tcccgctgtt cgtgcccatg tccttcttcg agagggaggc cgagcacgtc gagggcttca
     1621 gtcccgagct ggccgtggtc accatcggcg ggggcaagga gctggaggag cctctggcca
     1681 tccggcccac gtccgagacg gtcatgggga cctacttcgc caagtggatc cagagctacc
     1741 gggacctgcc cctgctggtg aaccagtggg ccaacgtcgt gcgctgggag ctgcgccctc
     1801 ggctgttcct gcgcacgacc gagttcctct ggcaggaggg ccacacctgc cacgccaccg
     1861 aagccgaggc ccgggacttc gcggtgcgca tcctccacga ggtctacgcc gactgcatgg
     1921 agcaggtcct ggccgtcccc gtctacaagg gccggaagac cgccgccgag cgcttcgccg
     1981 gggccatcaa cacgatggcg tgtgaggcga tgatgcggga cggcaaggcc ctgcagatgg
     2041 gcaccagcca cgagctcggg cagaacttcg cgaaggcctt cgggatcgag ttcctctcgg
     2101 ccgagggcaa ccaggagaac gtctggcaga cctcgtgggg cacctcgacc cgcatggtcg
     2161 gcggcctgat catgacccac ggtgacgaca acggcctgcg ggtcccgccc gtgctggcgc
     2221 ccatccaggt cgtcgtcctc gtcgtgcgtg acgaggaggg ggcgggggac gccgcggccc
     2281 ggctggcgga cgagctgaag gccgccggcg tccgggtccg cctcgatgac aaggtcgaca
     2341 cctccttcgg ccgccgggcc accaattggg agctcaaggg catccccctg cggatcgagg
     2401 tcggtccccg ggacctcaag gagggcgagg tgaccctcgt gcgccgcgac aacggcgaga
     2461 agcgccaggt cgccgtcacg ggtgtcgccg gcgaggtccc cggcctgctc gacacgatcc
     2521 aggccgacct cctggccggg gccacgaagg accgcgacga ccgcaccgtc gacgtgtcct
     2581 cgatcgagga cgtggccgag gccgcggcga acggcttcgt ccgcatcccg tgggacagcg
     2641 tcggcctcga gggcgagtcc cgcctggcca acgacgccct caccgttcgc tgcctccagc
     2701 gcgcggacgg cagcctcccc ggagccgacg acgagcccga cctcatcgcc ctcgtcgccc
     2761 gctcctactg acccgtaccc cagtcgttca gcgagaacga tgagtttgcg gggacatgag
     2821 agtcagagtg gtgacggacg cacctgggag gaaccgatga tcgatctgat gccggccaca
     2881 c
//
LOCUS       DASTFV010000394         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1442230, whole genome shotgun sequence.
ACCESSION   DASTFV010000394 DASTFV010000000
VERSION     DASTFV010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442230"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VFP02_07230"
     CDS             <1..979
                     /locus_tag="VFP02_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9542857.1"
                     /translation="FGEVDALSDLNLSVPEGQITVLLGPNGAGKTTAIRMITGALNPD
                     RGEIRTFGVDPGDEGEDVRRRCGVVSAKPALYDRLNGWDNLAYAAELYGLRDYDEHLR
                     EAAARFGIEAALQKMVGGYSTGMKTRLALARSIMHDPDLLLYDEPTSGLDPESSHAVL
                     ELIRSMTEDGHTVVMCTHLLLEAEGLADQVVVLEDGTDLISGTPKDLTDRYWPGVVVH
                     MDAEQPDMLDRLAHAEGVIAYQRNGVAELQLDDLGRVPDLVALLAAAGVRLTRVEPRI
                     PTLEDLYFAVRDGRAAFAEGNTLGGSDLAARFDRKRTAPSLNDLTEVDP"
     gene            976..1800
                     /locus_tag="VFP02_07235"
     CDS             976..1800
                     /locus_tag="VFP02_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9542858.1"
                     /translation="MSWSRIWTVTRTDLKQLIQAKDFWIPMLILGGIFFLFVPTILLL
                     SITAIGDVQAVAQISQALEVLPERAQAAVRGDTDAGRASYALAVYLFAPVAVVVPLTI
                     STAVGASTIVGERERGTGEFLAHSPAAVKEIYLGKLIASLLPGYATTLLGFGIYSMVV
                     NLIVGPEVGGWFFPTREWWVLMLWVVPPFLALTLSIVLRLSARVKSTAAAQQASGLVT
                     LPLILVAYSQSTGALFGAASASLWIGAVAWVVALAGLSRGMKVVTRARLLGVADGI"
     gene            complement(1822..>2188)
                     /locus_tag="VFP02_07240"
     CDS             complement(1822..>2188)
                     /locus_tag="VFP02_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542859.1"
                     /translation="FPHGLYHGRVTPATALALVHAYAEGRIHPAGFRGRAAWSPAAQQ
                     AEILLRHELEEWGVDALALDGHDHEGDRHTIRFSVDGDVHEVVVDVSIADHERLLACQ
                     NTALGRPRVITPVSIRSRG"
BASE COUNT          293 a          833 c          731 g          331 t
ORIGIN      
        1 cttcggcgag gtcgatgcgc tgtccgacct gaacctgtcg gtgcccgagg gccagatcac
       61 cgtcctgctc gggcccaacg gagcgggcaa gaccacggcc atccgcatga tcaccggcgc
      121 gctgaacccg gaccggggtg agatccggac gttcggcgtc gatcccggcg acgagggtga
      181 ggacgtccgc cggcgctgcg gcgtcgtgtc ggccaagccg gcgctgtacg accgcctcaa
      241 cggctgggac aacctggcct acgccgccga gctctacggc ctgcgggact acgacgagca
      301 cctccgggaa gcggccgccc gcttcggcat cgaagcggcc ctccagaaga tggtcggggg
      361 ctactcgacg ggcatgaaga cccgcctggc cctggcccgt tcgatcatgc acgaccccga
      421 cctgctgctc tacgacgagc ccacgtcggg cctggacccc gagtcgtccc acgcggtcct
      481 cgagctcatc cgctccatga ccgaggacgg ccacaccgtc gtcatgtgca cccacctcct
      541 cctggaggcc gagggcctgg ccgaccaggt cgtcgtcctc gaggacggca ccgacctcat
      601 ctcgggcacg cccaaggacc tgaccgaccg ctactggccc ggcgtcgtgg tccacatgga
      661 cgccgagcag cccgacatgc tcgaccgcct ggcgcacgcc gagggcgtca tcgcctacca
      721 acgcaacggt gtggccgagc tccagctcga cgacctgggc cgggtcccgg acctggtcgc
      781 cctgctggcc gccgccggcg tccgcctcac ccgggtcgag ccccgcatcc ccacgctcga
      841 ggacctctac ttcgccgtcc gcgacggccg ggccgccttc gccgagggca acaccctggg
      901 cgggtccgac ctggccgctc gcttcgaccg caagcgcacc gcgccatcgc tcaacgatct
      961 gacggaggtg gacccgtgag ctggagccgc atctggaccg tcacccgcac ggacctcaag
     1021 cagctgatcc aggccaagga cttctggatc cccatgctga tcctgggcgg gatcttcttt
     1081 ctcttcgtgc ccaccatcct cttgctctcg atcacggcca tcggcgacgt gcaggccgtg
     1141 gcccagatca gccaggccct ggaggtcctg cccgaacggg cccaggccgc ggtccggggt
     1201 gacaccgacg ccggacgggc gtcctacgcc ctcgccgtct acctgttcgc ccccgtggcc
     1261 gtggtcgtgc cgctgaccat ctcgaccgcc gtcggggcct cgaccatcgt cggcgagcgg
     1321 gagcggggca cgggcgagtt cctggcccac tcccccgccg cggtgaagga gatctacctc
     1381 ggcaagctca tcgccagcct cctgcccggc tacgccacga cgctgctcgg cttcgggatc
     1441 tactcgatgg tcgtgaacct gatcgtcggc cccgaggtcg gcggctggtt cttccccacc
     1501 cgggagtggt gggtgctgat gctctgggtc gtcccgccgt tcctggccct gaccctgtcg
     1561 atcgtcctcc gcctgtcggc gcgggtgaag tcgaccgccg ccgcccagca ggcctccgga
     1621 ctggtcaccc ttcccctgat cctggtcgcc tactcgcagt cgaccggcgc cctcttcggc
     1681 gccgcctcgg cctcgctctg gatcggcgcc gtcgcgtggg tcgtcgccct cgccggcctc
     1741 agccgcggca tgaaggtcgt cacccgggcc cgcctcctcg gcgtcgccga cggcatctga
     1801 cctccccccg gcgggcgccc gtcagcccct ggagcggatg gacacggggg tgatgaccct
     1861 cgggcggccg agggcggtgt tctggcacgc cagcaggcgc tcatggtcgg cgatcgacac
     1921 gtcgacgacg acctcgtgaa cgtctccgtc gaccgagaag cggatggtgt ggcggtcacc
     1981 ctcatggtcg tggccgtcca gcgcgagggc atcgacgccc cactcctcga gctcgtgacg
     2041 gaggaggatc tcggcctgct gggcggcggg tgaccaggcc gcccggcccc ggaagccggc
     2101 ggggtggatg cgaccctcgg cgtaggcgtg gaccagcgcg agcgcggtgg ccggtgtgac
     2161 ccggccgtgg tacaggccgt gcgggaac
//
LOCUS       DASTFV010000395         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1445381, whole genome shotgun sequence.
ACCESSION   DASTFV010000395 DASTFV010000000
VERSION     DASTFV010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445381"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1135
                     /locus_tag="VFP02_07245"
     CDS             38..1135
                     /locus_tag="VFP02_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9542860.1"
                     /translation="MGDQPVVPDYDGATVNNVVPALLGQLDQAPSWLPAVAVESDQVV
                     LLVLDGLGWDQLQERLALAPTMAGMEGGRICTVAPSTTACALTSISTGLSPGEHGIVG
                     YRMVVNDSILNVLRWATPDGDMRKVVPPTSIQSAAPFGGERPPVVTRAEFAHSGFTLA
                     HLSQTRLTPYRMASTLVTEVGRLVGAGEPFVYAYYEGIDKVSHEYGLADHYDAELRAT
                     DRLVADLLDTLPPGATLVVTSDHGQVDVGDNVITPAADVLRHVRLQSGDARFRWLHAR
                     SGQADALLDAATAHHGDTAWVVSLEQIEAEQWLGKLTPAAAGRLGDVALVAREPVAFD
                     DPNDSGPYHLIGRHGSLTSAEMWVPLLATQA"
     gene            1173..1682
                     /locus_tag="VFP02_07250"
     CDS             1173..1682
                     /locus_tag="VFP02_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HET9542861.1"
                     /translation="MSDVPTGNPNAAEPVAPELVTPTTDGETAGEGHNNVAEPAKVMR
                     VGTMIRQLLDEVRQTELDGPSRDRLREIYETSIAEIGGAVSPDLREELDRLALPFGEG
                     TPTDAELRIAQAQLVGWLEGLFHGIQATLFAQQMAARNQLEDMRRQLPPGGQPGAPGD
                     PPPGGGTYL"
     gene            1816..>2306
                     /locus_tag="VFP02_07255"
     CDS             1816..>2306
                     /locus_tag="VFP02_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET9542862.1"
                     /translation="MSFVHLHNHTEFSMLDGAARVGDAVAAAKADGQPALAITDHGNM
                     YGVLDFYKACRDAGITPIIGTEAYMAHESRAERPARRGRLDDSGGDTEGGRKLYYHLT
                     LLAQDRTGYRNMIQLASRAFLEGYFYKPRIDWELLDEHSEGLIATTACLGGHVLQALM
                     TGD"
BASE COUNT          348 a          873 c          766 g          319 t
ORIGIN      
        1 ggctgagtag gttgcccagc cgacggaagg atgccgggtg ggtgatcaac ccgtggtgcc
       61 cgattacgac ggcgccactg tgaacaacgt ggtgcccgcc ctgctgggac agctcgacca
      121 ggccccctcc tggctgcccg cggtcgccgt cgagagtgac caggtcgtgc tgctcgtcct
      181 cgacggcctc ggctgggacc agctccagga gcgcctggcc ctcgccccga cgatggcggg
      241 catggagggc ggtcgcatct gcaccgtggc ccccagcacg accgcctgcg ccctcacctc
      301 gatctccacc ggcctgtccc ccggcgagca cgggatcgtc ggctaccgca tggtggtcaa
      361 cgacagcatc ctcaacgtgc tgcgctgggc caccccggac ggtgacatgc gcaaggtcgt
      421 ccccccgacc tcgatccagt ccgccgcccc gttcggcggc gagcgcccgc cggtggtcac
      481 ccgggccgag ttcgcccact ccggcttcac gctggcccac ctcagccaga cccggctcac
      541 gccgtaccgc atggcgtcga ccctggtgac cgaggtcggc cgactcgtcg gggccgggga
      601 gcccttcgtc tacgcctact acgagggcat cgacaaggtg tcccacgagt acgggctggc
      661 cgaccactac gacgccgagc tgcgggccac cgaccgcctg gtggccgacc tgctcgacac
      721 cctccctccc ggcgcgaccc tggtcgtcac ctcggaccac ggccaggtcg acgtcggtga
      781 caacgtcatc acccccgccg ccgacgtctt gcgccacgtg cgtctccagt cgggcgacgc
      841 ccgcttccgg tggctgcacg cccgctccgg tcaggccgat gccctgctgg acgccgctac
      901 cgcccaccac ggcgacaccg cctgggtggt cagcctggag cagatcgagg ccgagcagtg
      961 gctcgggaag ctgacccccg cggcagccgg tcggctgggt gacgtggccc tcgtggcccg
     1021 ggagccggtg gccttcgacg accccaacga ctcggggccc tatcacctga tcgggcggca
     1081 cggctcgctc acgtcagccg agatgtgggt gccgttgctg gcgacgcagg cctagcgtcc
     1141 aaggtcccgc ccgctccgca gagaggccga tcatgtccga cgtccccacc ggcaacccga
     1201 acgccgccga gcctgtcgcc cccgagctgg tcacgcccac gaccgatggt gagaccgcag
     1261 gcgaaggcca caacaacgtg gccgagccgg ccaaggtcat gcgggtcggc accatgatcc
     1321 gccagctcct cgacgaggtc cgccagaccg agctcgatgg tcccagccgg gaccggctcc
     1381 gggagatcta cgagacctcc atcgccgaga tcggcggcgc ggtgtccccg gacctgcggg
     1441 aggagctgga ccgcctggcg ctgcccttcg gggagggcac gcccaccgac gccgagctcc
     1501 gcatcgccca ggcccagctc gtgggctggc tggagggcct gttccacggc atccaggcca
     1561 cgctgttcgc ccagcagatg gcggcccgca accagctcga ggacatgcgc cgccagctgc
     1621 cccccggcgg ccagcccggt gcgccgggtg accccccgcc gggcggcggg acctacctct
     1681 agacaggttt cttgcccacc ggcggtgggc aggttacggt cgccgctggg aatcacacgg
     1741 gtgtgatttc ctccacaggc tgtggaaacc gcctgtgggc cgagtgggga aatgtttgcg
     1801 accgaatcgt ccggaatgtc cttcgtccac ctccacaacc acaccgagtt ctcgatgctc
     1861 gacggtgcgg cgcgggtggg cgacgcggtg gccgcggcca aggccgacgg ccaacccgcg
     1921 ttggccatca ccgaccacgg caacatgtac ggggtcctgg acttctacaa ggcctgtcgt
     1981 gacgccggga tcacgccgat catcggcacc gaggcctaca tggcccacga gagccgggcc
     2041 gagcgcccgg cgcgccgggg ccggctggac gactccgggg gcgacaccga gggtggccgg
     2101 aagctctact accacctgac cctgctggcc caggaccgga ccggctaccg gaacatgatc
     2161 cagctggcca gccgggcctt cctcgagggg tacttctaca agccccgcat cgactgggag
     2221 ctcctcgacg agcacagcga aggcctcatc gccaccacgg cctgcctcgg tggccacgtc
     2281 ctgcaggccc tgatgacggg tgacga
//
LOCUS       DASTFV010000396         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1451793, whole genome shotgun sequence.
ACCESSION   DASTFV010000396 DASTFV010000000
VERSION     DASTFV010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451793"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFP02_07260"
     CDS             complement(<1..346)
                     /locus_tag="VFP02_07260"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET9542863.1"
                     /translation="MLAANEAAWRWQPHPEVWLLVLGVVALSLYVTRVIGPKVVPAGR
                     PVLSRRQAVCLVAGILTLWLASDWPLHDLGEEYLFSLHMTQHLLLSYVMPLLFLLATP
                     PWLARLVVPEGSA"
     gene            complement(353..700)
                     /locus_tag="VFP02_07265"
     CDS             complement(353..700)
                     /locus_tag="VFP02_07265"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HET9542864.1"
                     /translation="MSSTDALTDEEYEALPTEPDQTGAHHPTELLYVKVAALLAVITL
                     AEISTYWFDFGDFLIPALITMMVVKFSLVIGYFMHLRFDNRIFTRVFLTGLFIAIAVY
                     IVMLTTFHYWQNP"
     gene            complement(708..1319)
                     /locus_tag="VFP02_07270"
     CDS             complement(708..1319)
                     /locus_tag="VFP02_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET9542865.1"
                     /translation="MTDATMAAPGEPHAGGHATSTGLSNEKLGMWVFLSSECLLFGGL
                     ISTYLLLHDRSVEGPTPQDVYDIPFTSVSSFVLLMSSLTMVLAVSALTRGDMRRNRLW
                     LLTTALLGTIFIGGQVYEFTSFVREGLGFTTNIFGSAFYTLTGFHGVHVTVGILMLLS
                     LVGLSLRGTLSQERAETVEIIGLYWHFVDIIWIVIFTVVYLAA"
     gene            complement(1312..>2450)
                     /locus_tag="VFP02_07275"
     CDS             complement(1312..>2450)
                     /locus_tag="VFP02_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET9542866.1"
                     /translation="SRKPIFGYPFMVASGIAIGFMGWGVWAHHMFVSGLGPISVAAFA
                     LTTMFIAVPTGVKILNWMATMWGGKLEFTTAMLFAIGLVGMFTIGGLSGVTHAVAPAD
                     TQQTDTYYIVAHFHYVLFGGAMMGLFSGFYFWWPKIFGHVLNEKLGKVHFWLMFIGFN
                     LTFGPMHILGLQGMSRRIYTYEEGLGFDLWNMVSSIGAFTIAVSVLVFLANVVRSRLH
                     AASAPPPGPDPWDARSLEWMLPSPTPAHNFDEIPTVTRLDEFWHRKYGTDENGRTVRI
                     AETEEVAQKGDATDVHLPSPSYWPLVVALGLPLIGYGLIFNLAWAVPGVLLVLGGIYG
                     WGLEPADDDENPPHHHAPADALAAGDGPAAELEAAPETEEVASD"
BASE COUNT          458 a          742 c          907 g          343 t
ORIGIN      
        1 aagccgaccc ctcggggacc accaggcggg ccagccaggg cggcgtggcc agcaggaaga
       61 gcagcggcat cacgtagctc agcaacaggt gctgggtcat gtggagcgag aagaggtact
      121 cctcgccgag gtcgtgcagg ggccagtccg aggccagcca gagggtcagg atcccggcga
      181 ccaggcagac cgcctgccgg cgactcagca cgggccggcc ggcgggcacg accttcgggc
      241 cgatgacccg ggtcacgtag agcgagaggg cgacgacccc gaggaccagg agccagacct
      301 cggggtgcgg ctgccagcgc caggcggctt cgttggcggc cagcaccggg acctacgggt
      361 tctgccagta gtggaaggtg gtgagcatga cgatgtacac cgcaatggcg atgaacagcc
      421 cggtcaggaa cacccgggtg aagatccggt tgtcgaagcg caggtgcatg aagtacccga
      481 tgaccagaga gaacttcacg accatcatcg tgatcagggc cgggatgagg aagtcgccga
      541 agtcgaacca gtaggtgctg atctcggcca gggtgatgac ggcgaggaga gcggcgacct
      601 ttacgtagag cagctcggtc gggtggtggg ccccggtctg gtccggctcg gtcggcagcg
      661 cctcgtattc ctcgtcggtc agggcgtcgg tgctgctcat cgtgacctca ggctgccagg
      721 tagacgacgg tgaagatgac gatccagatg atgtccacga agtgccagta gaggccgatg
      781 atctcgaccg tctcggcccg ttcctggctg agggtccctc ggagggacag accgacgagc
      841 gacaggagca tgaggatgcc cacggtcacg tggaccccgt ggaacccggt cagcgtgtag
      901 aaggccgagc cgaagatgtt ggtggtgaac ccgaggccct cccgcacgaa cgaggtgaac
      961 tcgtagacct ggccaccgat gaagatcgtg cccagcagag cggtggtcag cagccacagc
     1021 cggttccggc gcatgtcgcc ccgggtcaag gccgacacgg ccagcaccat ggtcagcgag
     1081 ctcatgagga gcacgaacga ggagacggac gtgaagggga tgtcgtagac gtcctggggc
     1141 gtcggcccct cgaccgaccg gtcgtgcaga agcaggtagg tggagatgag cccaccgaac
     1201 agcaggcact ccgaggacag gaacacccac atccccagct tctcgttgga gaggccggtg
     1261 ctggtggcgt ggccccccgc gtgcggctcg cccggggcgg ccatcgtggc gtcagtcact
     1321 ggcgacctcc tcggtctcgg gcgcagcctc caactcggcc gcggggccgt cgccggcggc
     1381 gagggcatcg gccggagcgt ggtggtgcgg cgggttctcg tcgtcgtcgg cgggctcgag
     1441 gccccagccg tagatcccac ccagcaccag gagcacgccg gggacggccc aggccaggtt
     1501 gaagatcagg ccgtagccga tcagggggag gcccagcgcg acgaccaggg gccagtagga
     1561 cggtgacggc aggtggacgt cggtggcgtc gcccttctgg gccacctcct cggtctcggc
     1621 gatgcgcacg gtgcgcccgt tctcgtcggt gccgtacttg cggtgccaga actcgtcgag
     1681 ccgggtgacc gtcgggatct cgtcgaagtt gtgggccggg gtgggcgacg ggagcatcca
     1741 ctcgaggctg cgggcgtccc acgggtcggg gcccggcggc ggggcgctgg cggcgtggag
     1801 ccggctgcgg accacgttgg ccaggaagac caggacggag acggcgatcg tgaaggcccc
     1861 gatcgacgag accatgttcc agaggtcgaa gccgaggccc tcttcgtacg tgtagatccg
     1921 gcgggacatg ccctgcaggc cgaggatgtg catgggcccg aacgtcaggt tgaagccgat
     1981 gaacatcagc cagaagtgga ccttgcccaa cttctcgttg aggacgtggc cgaagatctt
     2041 gggccaccag aagtagaagc cggagaagag gcccatcatc gccccgccga agaggacgta
     2101 gtggaagtgg gcgacgatgt agtaggtgtc ggtctgctgg gtgtcggccg gggccacggc
     2161 gtgggttacg cccgacagcc caccgatggt gaacatgccc accagcccga tggcgaacag
     2221 catcgcggtg gtgaactcga gcttgccccc ccacatggtg gccatccagt tgaggatctt
     2281 caccccggtg ggcacggcga tgaacatggt cgtcagggcg aaggccgcca ccgagatggg
     2341 cccgagcccg gagacgaaca tgtggtgggc ccacacgccc cagcccatga agccgatggc
     2401 gatgcccgag gcgaccatga acgggtaccc gaagatgggc ttgcggctga
//
LOCUS       DASTFV010000397         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1458491, whole genome shotgun sequence.
ACCESSION   DASTFV010000397 DASTFV010000000
VERSION     DASTFV010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458491"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VFP02_07280"
     CDS             complement(<1..872)
                     /locus_tag="VFP02_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9542867.1"
                     /translation="MTAILNEAERIGEVDAAAAATSSWRLLARRFRRHRPGMVGLAIL
                     LVVIAMAVFAPLLAPYPPNQLSDLINTGPSTAHWLGTDDVGRDLLSRVIFGARNSLIA
                     CTGIVALAVAFALPLALLAGYRGGRTDTVIMRLTDAAFAFPPLVVALAIVSLRGNSMF
                     NLVLAIGLVFIPSLIRLIRGQVLAVREETYIEAARSVGTPSGRIAARHVLPNVASPLI
                     IQVAVSLGFALLAEAGLAFLGAGPPPPTATWGRMLQTAFQYVLVVPWQIFVPGAAITL
                     TVLAFNLIGDGLRDS"
     gene            complement(869..1816)
                     /locus_tag="VFP02_07285"
     CDS             complement(869..1816)
                     /locus_tag="VFP02_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9542868.1"
                     /translation="MGAYIARRLLGLIPLLLLISFAVFALVLAVPGDPARTIAGGLDA
                     SDTRVDEIRDELGLDDPLLEQYGRWLGGVLSGDLGSSLFNGRTVASELAARFPVTFSL
                     ALGAIVFAAAVGIPLGLLAGTRPGSLLDRALSVGTSFGIALPDFFLATALVVIFAVEL
                     DVLPAIHYVDLAVSPIEWAKHLVMPWIALGVASAASLARQVRGSMIEVLDADYIRTAR
                     SKGLAERRVLGRHALKNALTPALTILGLQFAYLLGGTFIIESIFALPGIGQYMLEAIT
                     SRDLPVIQGVVLLVAVIFVFINLVVDILYGVLNPKVRVT"
     gene            complement(1835..>2385)
                     /locus_tag="VFP02_07290"
     CDS             complement(1835..>2385)
                     /locus_tag="VFP02_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9542869.1"
                     /translation="VYERDLDKARDLLAEAGVPDGFEFDLAIPGGVTSQERQAELIQS
                     QLAEVGIVANIVPLGTDIATEYYLQHNGNAFSAARPASADPVGQVQGQWANFQFVAIN
                     NNAERQDLTDLVEQAQLAPTDEERAAIMQQAMVIIVDEALEVPIAFTPRNVAWSNERI
                     EGEIGAPAEICDPVDLQGISLR"
BASE COUNT          394 a          759 c          928 g          304 t
ORIGIN      
        1 gagtcccgca ggccgtcacc gatcaggttg aaggccaaca ccgtgagggt gatcgccgcg
       61 cccgggacga agatctgcca cgggaccacg aggacgtact ggaacgcggt ctggagcatc
      121 cggccccagg tcgcggtggg cggtggcggg ccggccccca ggaacgccaa gccggcctcg
      181 gccagcaggg cgaagccgag gctgaccgcg acctggatga tcagcgggga ggccacgttg
      241 ggcagcacat ggcgggcggc gatgcgcccg gacggggtgc ccaccgatcg ggcggcctcg
      301 atgtaggtct cctcgcggac ggccagcacc tggccccgga tgaggcggat caggctgggg
      361 atgaagacca gcccgatggc gaggacgagg ttgaacatgc tgttgccgcg cagggacacg
      421 atcgccagcg cgaccacgag cggggggaag gcgaaggcgg cgtcggtcag ccgcatgatc
      481 acggtgtcgg tccggccgcc ccggtaaccg gcgaggaggg cgaggggcag ggcgaaggcc
      541 acggcgaggg ccacgatccc ggtgcaggcg atcagcgagt tccgggcccc gaagatcacc
      601 cggctcagga ggtcccggcc gacgtcgtcg gtgcccagcc agtgggcggt ggagggcccg
      661 gtgttgatca ggtcggacag ctggttgggg ggatagggcg cgagcagggg ggcgaacacg
      721 gccatggcga tgaccacgag gaggatggcc aggcccacca tgcccgggcg gtgccggcgg
      781 aagcggcggg ccaggaggcg ccaggagctg gtcgccgccg cggcggcgtc gacctcgccg
      841 atccgttcgg cctcgttcag gatcgccgtc acgtgacccg gaccttcggg ttgaggacgc
      901 cgtagaggat gtccaccacc aggttgatga acacgaagat gacggcgacg agcagcacga
      961 cgccctggat caccggcagg tcccggctgg tgatggcctc cagcatgtac tgcccgatgc
     1021 cggggagggc gaagatcgac tcgatgatga acgtcccgcc cagcaggtag gcgaactgca
     1081 ggccgaggat ggtcagcgcc ggggtgaggg cgttcttgag ggcgtggcgg cccaggaccc
     1141 gacgctcggc caggcccttg gaccgggcgg tgcggatgta gtcggcgtcg agcacctcga
     1201 tcatcgaccc ccggacctgg cgggccagcg aggcggcgga ggcgacgccg agggcgatcc
     1261 acggcatgac caggtgcttg gcccactcga tcgggctcac ggcgaggtcg acgtagtgga
     1321 tggcgggcag gacgtcgagc tccacggcga agatcacgac caacgccgtg gccaggaaga
     1381 agtcggggag ggcgatcccg aagctggtcc ccacgctgag ggcgcggtcc agcagcgagc
     1441 cgggccgggt gccggcgagg agaccgaggg ggatgcccac cgccgcggcg aacacgatgg
     1501 cgccgagggc gaggctgaag gtcactggga agcgggcggc cagctcgctg gcgaccgtgc
     1561 gcccgttgaa cagcgacgag cccaggtcac cggacagcac gccgccgagc cagcggccgt
     1621 actgctcgag caacgggtca tcgaggccga gctcgtcgcg gatctcgtcc acccgggtgt
     1681 cggacgcgtc cagcccaccg gcaatggtcc gggccgggtc gccggggacc gccagcacca
     1741 gcgcgaagac ggcgaagctg atcagcagca gcaggggtat gagccccagc aggcgacggg
     1801 cgatgtaggc gcccatggtg tcagcggccc cgggtcagcg gaggctgatg ccctgcaggt
     1861 ccacgggatc acagatctcg gccggggccc cgatctcgcc ctcgatgcgc tcgttgctcc
     1921 acgccacgtt ccggggcgtg aaggcgatcg ggacctcgag ggcctcgtcg acgatgatca
     1981 ccatcgcctg ctgcatgatc gcggctcgtt cctcgtccgt gggggcgagc tgggcctgct
     2041 cgaccagatc agtgaggtcc tggcgctcgg cgttgttgtt gatggccacg aactggaagt
     2101 tggcccactg gccctggacc tggccgaccg ggtccgctga ggccggacgg gcggccgaga
     2161 aggcgttgcc gttgtgctgc aggtagtact cggtggcgat gtcggtgccg agggggacga
     2221 tgttggccac gatgccgacc tcggccagct ggctctggat cagctcggcc tggcgctcct
     2281 ggctggtgac cccgcccggg atggccaggt cgaactcgaa gccgtcaggc accccggctt
     2341 cggccaacag gtcccgagcc ttgtccaggt cccgctcgta gacgc
//
LOCUS       DASTFV010000398         3491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1461381, whole genome shotgun sequence.
ACCESSION   DASTFV010000398 DASTFV010000000
VERSION     DASTFV010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461381"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VFP02_07295"
     CDS             complement(<1..240)
                     /locus_tag="VFP02_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9542870.1"
                     /translation="MVRSDVLAEVQLFADFPAEGVAEVAAAAVERQLERNDVLFNEQD
                     EAHELFVVLDGRIAIANVALDGRESVVALMEAGDLF"
     gene            297..920
                     /locus_tag="VFP02_07300"
     CDS             297..920
                     /locus_tag="VFP02_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET9542871.1"
                     /translation="MGSVWTIVVAAGAGQRYGGAKQYERLGDRRVIDWSLATAAAATD
                     GVVAVVAEAAPVEVVADHTVTGGLTRSASVRAGLAAVPEAADVIVVHDAARPLASPGL
                     FAAVIEAVRDGADAALPAVPVTDTIRHRDGSPVDRDELVAVQTPQAFAAGRLRAVHAP
                     EPEATDDASLVEAAGGTVVVVAGESRNAKITSPDDLVVAAALLGAPA"
     gene            917..1393
                     /gene="ispF"
                     /locus_tag="VFP02_07305"
     CDS             917..1393
                     /gene="ispF"
                     /locus_tag="VFP02_07305"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET9542872.1"
                     /translation="MSVRVGQGYDVHPFSEDPARPLVLGGVTVDGSPGLAGHSDADAL
                     AHACADAVLGAAGLGDLGDHFPDTDPQWSGADSLTLLAEVVRLAAEAGWRVVNLDATI
                     IAERPKVGPLRPQMADRLTAVVGAPVSVKATTHEGLGAIGRGEGIACLAVALLEAD"
     gene            1393..2268
                     /gene="rlmB"
                     /locus_tag="VFP02_07310"
     CDS             1393..2268
                     /gene="rlmB"
                     /locus_tag="VFP02_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET9542873.1"
                     /translation="MAARRPPRGGGKGRRPPASSSGTRSGSARSGPPRSRGPKRSGSS
                     GPPGLGGDQVEGRQAVREALLGPRTVREILVAADMDPAPILDDIRDLAHEQRVAIREL
                     KRSTLEAQARSEAPQGVVAMVSPIQPVDLEVLAAVDRPFLLVLDGLHDPQNLGAILRT
                     AEAAGVTGVVLPRHRSVRVTAAVTKTAAGAVEWLPMAVVAGVPTALTELKAKDVWVVG
                     LDQDADQGFWGLSVANDPVALVLGSEGSGLARLTRERCDVVAALPLAGRLGSLNVSAA
                     AAVACYEIARLRQGG"
     gene            complement(2281..>3491)
                     /locus_tag="VFP02_07315"
     CDS             complement(2281..>3491)
                     /locus_tag="VFP02_07315"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET9542874.1"
                     /translation="PFRGAGRPEAAAAIERAMDLFAFEIEMDPAEVRRRNLLADDVYP
                     YSTPGGAVYDIGAYTTALDMALEAADYAGLRAEQARRREAGDTRQLGIGLSVYVEITA
                     LGGGGEFGTVTALPDGSVEVRTGATPFGQGHDTTWKMLASDRLGVPMEAIRVIHGDTD
                     DVAAGSTTGGSRSVQIAGSSILSASAHLIDRAREQAAELLEASVDDVVLDTASGQFHV
                     AGVPAVGVSWGEVAGQSGEPLMGASDFTAEGPTFPFGAHVAVVEVDTETGAVVLTRLI
                     AVDDAGTLINPMLADGQIHGGLAQGAAQALFEEMAYDEDGNPQTSNLADYTVPAASEV
                     PSFERIEMVTPTPLNELGAKGIGESGSIGSTPAVQNAVIDAVSHLGVRHIDMPCTPER
                     VWRAIQAAPG"
BASE COUNT          427 a         1303 c         1289 g          472 t
ORIGIN      
        1 gaagaggtca ccggcctcca tgagggcgac caccgactcc cgaccgtcca gggccacgtt
       61 ggcgatggcg atgcgcccgt cgagcacgac gaagagctcg tgggcctcgt cctgctcgtt
      121 gaacagcacg tcgttgcgct cgagttggcg ctcgaccgcg gcggcggcca cctcggccac
      181 gccctcggcg gggaagtcgg cgaagagctg gacttcggcg aggacgtctg agcgaaccat
      241 ggcggcaggc tagtgacgta ggtcacggac ctggtccggc gggtaccgtc cggcgcgtgg
      301 gcagcgtctg gacgatcgtg gtagccgccg gcgccggcca gcggtacggc ggggccaagc
      361 agtacgagcg cctgggcgac cgtcgggtca tcgactggtc gctggccacc gccgcggccg
      421 ccaccgacgg cgtggtcgcc gtggtggctg aggccgctcc tgtcgaagtc gtcgccgatc
      481 acacggtcac cggtggcctg acccgctcgg cctcggtccg ggccggcttg gctgcggttc
      541 cggaggcggc tgacgtgatc gtggtccacg atgccgcccg gcccctggcc tcgcccggcc
      601 tcttcgccgc cgtgatcgaa gccgtgcggg acggggccga tgccgccctg cccgccgtgc
      661 cggtgaccga caccatccgc caccgcgacg gcagcccggt ggaccgcgac gagctggtgg
      721 cggtccagac cccacaggcg ttcgcggccg gccggctgcg ggccgtccac gcgccggagc
      781 cggaggccac cgacgacgcc tcgctggtcg aggcggcggg aggcaccgtc gtggtcgtcg
      841 cgggggagtc ccgcaacgcc aagatcacct cgcccgacga cctcgtggtc gccgccgccc
      901 tgctcggggc cccggcgtga gcgtccgcgt cggccagggc tacgacgtcc accccttcag
      961 cgaggatcct gcccgaccgc tggtcctcgg cggggtcacc gtcgacggga gcccgggcct
     1021 ggccggccac agcgacgccg acgccctcgc ccacgcctgt gccgacgccg tcctcggagc
     1081 ggccggcctg ggtgacctcg gcgatcactt cccggacacc gatcctcagt ggtccggcgc
     1141 cgacagcctc acgctgctgg ccgaggtcgt gcgcctcgcc gccgaggccg gctggcgggt
     1201 ggtcaacctc gacgccacca tcatcgccga gcggccgaag gtgggcccgc tgcgaccaca
     1261 gatggcggac cgtctcacgg cggtggtcgg cgcgcccgtc tcggtgaagg ccaccaccca
     1321 cgagggcctc ggcgccatcg gtcgtggcga gggcatcgcc tgcctcgccg tggctctcct
     1381 cgaggcggac tgatggcggc ccgacgcccg cctcggggcg ggggcaaggg tcggcgcccc
     1441 cccgcgagca gctccggcac ccgttccggc tcggcccgtt ccggcccacc ccgcagccgc
     1501 ggtccgaagc gctcgggcag cagcggcccg ccaggcctcg ggggcgacca ggtcgaaggt
     1561 cgtcaagccg tccgggaggc cctgctcggc ccccgcacgg tccgggagat cctcgtcgcc
     1621 gcggacatgg atcccgctcc gatcctcgac gacatccggg acctggccca cgagcagcgg
     1681 gtcgccatcc gggagctcaa gcgctcgacc ctggaagccc aggcccgctc cgaggccccc
     1741 cagggggtcg tggcgatggt cagcccgatc cagcccgtgg acctcgaggt cctggccgcg
     1801 gtggaccgcc cgttcctcct ggtcctcgac ggcctccacg acccccagaa cctgggcgcg
     1861 atcctgcgca cagccgaagc cgcgggggtg accggggtcg tgctgccccg gcaccggtcg
     1921 gtccgggtga ccgccgcggt gacgaagacc gcggccggcg ccgtggagtg gctgccgatg
     1981 gccgtcgtgg ccggtgtccc gacggcgctg accgagctca aggcgaagga cgtctgggtc
     2041 gtcggcctgg accaggacgc cgaccagggg ttctggggcc tgtcggtggc caacgacccg
     2101 gtggccctgg tcctgggcag cgagggctcc ggcctggccc gtctcacccg cgagcgctgt
     2161 gacgtggtcg ccgccctccc gctggccggc cgcctcgggt ccctgaacgt cagcgccgcc
     2221 gccgccgtcg cctgctacga gatcgcccgc ctccgccagg ggggctgagc gctcggaggc
     2281 tcagcccggg gccgcttgga tggcgcgcca gacgcgctcg ggggtgcagg gcatgtcgat
     2341 gtggcggacg ccgaggtggc tgacggcgtc gatcaccgcg ttctgcaccg ccggcgtgga
     2401 gccgatgctg ccggactcgc cgatgccctt ggcgccgagc tcgttcagcg gcgtgggggt
     2461 gaccatttcg atgcgctcga agctggggac ctcgctggcc gcggggacgg tgtagtcggc
     2521 caggttggag gtctgcgggt tgccgtcctc gtcgtaggcc atctcctcga agagggcttg
     2581 ggccgcgccc tgggccaggc ccccgtggat ctggccgtca gccagcatgg ggttgatcag
     2641 cgtgcccgcg tcgtcgacgg cgatcaggcg ggtgagcacg acggcgccgg tctcggtgtc
     2701 cacctctacg acagccacat gggccccgaa agggaaggtc ggaccttcgg cggtgaagtc
     2761 cgaggcgccc atgaggggct ccccggactg gcccgccacc tcgccccagc tcacgcctac
     2821 ggcggggacg ccggccacat ggaactggcc gctggcggtg tcgagcacga cgtcgtcgac
     2881 cgacgcctcc agcagctcgg ccgcctgctc ccgggcccgg tcgatcaggt gggccgaggc
     2941 cgacaggatc gacgagccgg cgatctgcac ggagcgcgag ccgccggtcg tgctgcccgc
     3001 ggccacgtcg tcggtgtcgc cgtggatgac ccggatcgcc tccatgggca cgcccaagcg
     3061 gtccgaagcc agcatcttcc aggtcgtgtc gtggccctga ccgaagggcg tggccccggt
     3121 gcggacctcg acggagccgt cgggcagcgc ggtgaccgtg ccgaattcgc cgccaccgcc
     3181 gagggcggtg atctccacgt agacgctcag cccgatgccg agctggcggg tgtcccccgc
     3241 ctcgcgccgg cgggcctgct cggcccgtag tccggcgtag tccgcggcct cgagcgccat
     3301 gtccagcgcg gtcgtgtagg ccccgatgtc gtacaccgcc ccgccgggcg tgctgtacgg
     3361 gtacacgtcg tcggccagca ggttcctccg gcgcacctcg gccggatcca tctcgatctc
     3421 gaacgcgaac aggtccatgg cccgctcgat ggcggcggcg gcctccggcc gaccggcccc
     3481 acggaacggg c
//
LOCUS       DASTFV010000399         2993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1466076, whole genome shotgun sequence.
ACCESSION   DASTFV010000399 DASTFV010000000
VERSION     DASTFV010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466076"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFP02_07320"
     CDS             complement(<1..579)
                     /locus_tag="VFP02_07320"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9542875.1"
                     /translation="MGVGATGARAARQLASTDAVDAVVVADIDPATRRKVADLLGSGA
                     ESSDALVPEADVVVLATPAGTQGDLGTQALRQGSSVVSTSDDVGDVRVLLDLDPEAIE
                     RGATMLVGAGFAPGLSCLLARHASAVLDAVDEVHVAKVGTGGPACAEQHHRALGEDGL
                     DWRDGGWVARTGGSGRELCWFPEPVGGRDCYRA"
     gene            complement(591..728)
                     /locus_tag="VFP02_07325"
     CDS             complement(591..728)
                     /locus_tag="VFP02_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542876.1"
                     /translation="MQRLRRVFLAIGVAGLFAALFRVRGKGGTPPSDGGWRQLEGPEL
                     R"
     gene            790..1182
                     /locus_tag="VFP02_07330"
     CDS             790..1182
                     /locus_tag="VFP02_07330"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET9542877.1"
                     /translation="MTVRHVNPEGMHQSPAFSQAVVVESPTKTIYIGGQNGVGPDGQV
                     VGDTLAAQSAQAFANLATILEAEGAGLADVVAWTIVVVDGHPIEEGVGAFQAVWDPAD
                     PPPAISVNVVAAMANPDFLVEITAIAAL"
     gene            complement(1200..1276)
                     /locus_tag="VFP02_07335"
     tRNA            complement(1200..1276)
                     /locus_tag="VFP02_07335"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1240..1242),aa:Ile,seq:gat)
     gene            1348..2019
                     /locus_tag="VFP02_07340"
     CDS             1348..2019
                     /locus_tag="VFP02_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9542878.1"
                     /translation="MELLAQGRDCDIYDCGDGTVLRRSRKAYDQTHEARVLRYAAEHG
                     YPVPTVHELVEDGRDLIMEKVAGPTMVEAIQRRPWQAGAIGRQLADLHARLHEIPGPD
                     WLPALPGGDTLLHFDLHPMNVILAPSGPVVIDWTNATRGPAGYDLARAWVLMASADTK
                     VIGPLRLVLTQVRRRLVDGFVDAAGREEARAGLPFAVELTLLDPNISDAEKARMRALA
                     EVEGR"
     gene            complement(2016..2678)
                     /locus_tag="VFP02_07345"
     CDS             complement(2016..2678)
                     /locus_tag="VFP02_07345"
                     /inference="COORDINATES: protein motif:HMM:NF023511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HET9542879.1"
                     /translation="MTTLDDDAPEDALLDDDLDDGDSDDDAVDHDPEDTLVNVPDADI
                     VADAVEMDDQPEVDLLSDGPYAADETELVVPPAKTKKPSRRQHRAEMKASARRVRRVV
                     RHIDTWSVFKVATIFVACIWGVVVLASLLVWRAAVTSGSVENTEDFIVDLGFEDFNFN
                     PQQMFEALLSAGAVVAIAAIFFIWLLTVLFNLICDITGGLRITMIEQDLADARKRRSR
                     KG"
     gene            complement(2680..>2993)
                     /locus_tag="VFP02_07350"
     CDS             complement(2680..>2993)
                     /locus_tag="VFP02_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET9542880.1"
                     /translation="TSEMAAAVARAGSLLGEPVPISSGWRSRAQQAALWARRHTNPYP
                     VARPGTSMHERGRAIDVPRSFVARLRLVATAAGLCQPLPSTDPVHFELCRRTLVTIMA
                     T"
BASE COUNT          453 a         1063 c         1079 g          398 t
ORIGIN      
        1 ggcccggtag cagtcccgcc cccccacggg ctcggggaac cagcacagct cacggcccga
       61 gccacccgta cgagccaccc agccgccgtc tcgccagtcc aggccgtcct cacccagtgc
      121 ccggtggtgc tgttcggcgc aggccgggcc gccggtgccg accttggcca cgtggacctc
      181 gtcgacggcg tcgagcacgg cggaggcgtg gcgggccaac aggcagctga ggccgggggc
      241 gaagccggcc ccgaccagca tggtggcccc ccgctcgatg gcctccgggt cgaggtcgag
      301 caggacgcgg acatcgccca catcgtccga cgtggacacg accgagctgc cctgacgcag
      361 ggcctgggtc cccaggtcgc cctgggtccc ggccggagtg gccagcacga cgacatcggc
      421 ctccgggacc agggcgtcgg aggactccgc gccgctcccg aggaggtcgg cgaccttgcg
      481 gcgggtcgcc ggatcgatgt cggccaccac gacggcgtcg acggcgtcgg tggaggccag
      541 ctgtcgggcc gcccgggccc cggtggcccc gacgcccacc accgcgatcc tcaacggagc
      601 tcggggccct cgagctgacg ccaaccgccg tcgctcggcg gggtaccgcc cttgccccgg
      661 acccggaaca aggcggcgaa gaggccggcc accccgatcg cgaggaagac ccggcggaga
      721 cgctgcatga ggccccggac ggtactaccg gtcggtgcca tcgccctccc ggcccgcaag
      781 actggcgcca tgaccgtgcg ccacgtgaac cccgagggca tgcaccagag ccctgccttc
      841 agccaagccg tcgtcgtgga gtcgccgacg aagacgatct acatcggcgg ccagaacggc
      901 gtcggccccg acggtcaggt ggtgggcgac acgctcgccg cccagtcggc ccaggccttc
      961 gccaacctgg ccacgatcct cgaggcggag ggagccggcc tggccgacgt cgtggcctgg
     1021 acgatcgtgg tcgtggacgg gcacccgatc gaagaggggg tcggtgcgtt ccaggcggtg
     1081 tgggacccgg ccgacccgcc gccggccatc agcgtgaacg tcgtcgccgc catggccaac
     1141 ccggacttcc tggtggagat caccgccatc gccgccctct gacggctccc ggaacatggt
     1201 ggtggggcta gttggaatcg aaccaacgac ctcgccctta tcagaggcgc gctctaaccg
     1261 actgagctat agccccgggg agcagacact gtaccgagcg gcccgcggga gaatcaccct
     1321 cgttgcggtt gctgcgagac tcccgcgatg gagctgttgg ctcagggccg ggactgcgac
     1381 atctatgact gcggcgacgg cacggtgctg cggcggtccc ggaaggccta cgaccagacc
     1441 cacgaggccc gggtcctgcg gtacgccgcc gagcacggtt acccggtgcc gacggtccac
     1501 gagctggtcg aggacggtcg ggatctcatc atggagaagg tggccggccc gacgatggtc
     1561 gaggccatcc agcgccgccc gtggcaggcc ggcgccatcg gccgccagct ggccgacctc
     1621 cacgcccggc tccacgagat cccgggaccg gactggctgc ccgccctgcc cggcggcgac
     1681 accctgctcc acttcgacct gcacccgatg aacgtgatcc tggccccgtc cgggccggtg
     1741 gtgatcgatt ggaccaacgc cacccggggc ccggcgggct acgacctggc ccgggcctgg
     1801 gtgctgatgg cctcggccga cacgaaggtc attgggccgc tccggctcgt gctgacccag
     1861 gtccgccgac gcctggtcga cgggttcgtc gacgccgccg ggcgagagga ggcccgggcc
     1921 ggcctgccct tcgcggtcga gctgaccctg ctcgacccca acatctctga cgccgagaag
     1981 gcccgaatgc gggccctggc ggaagtcgag ggacgttagc ccttgcggga ccgtcgcttg
     2041 cgggcgtcgg ccaggtcctg ctcgatcatg gtgatccgga gcccgcccgt gatgtcgcag
     2101 atcaggttga acaggaccgt cagcagccag atgaagaaga tggcggcgat ggcgaccacc
     2161 gccccggccg agagcagggc ctcgaacatc tgctgcgggt tgaagttgaa gtcctcgaag
     2221 ccgagatcga cgatgaagtc ctcggtgttc tccacggagc cggaggtcac cgcggcccgc
     2281 cagaccagca gcgaggccag cacgaccacg ccccagatgc aggccacgaa gatggtggcc
     2341 accttgaaga ccgaccacgt gtcgatgtgg cggaccaccc ggcgcacccg gcgggcggac
     2401 gccttcatct ccgcccggtg ctgccggcgt gagggcttct tggtcttggc ggggggcacg
     2461 accagctcgg tctcgtcggc ggcatagggc ccgtcgctga gcagatcgac ctcgggttgg
     2521 tcatccatct cgacggcgtc ggccacgatg tcggcgtcgg ggacgttcac gagggtgtcc
     2581 tcggggtcgt ggtcgaccgc gtcgtcgtcg gagtcgccgt cgtcgaggtc gtcgtcgagc
     2641 aaggcgtcct caggggcgtc gtcgtcgagg gtcgtcacgt cacgtcgcca tgatcgtgac
     2701 cagtgtccgc cggcacagtt cgaagtgtac ggggtcggtc gaaggcaagg gttggcacag
     2761 ccccgcggcg gtggccacga ggcgcaggcg agccacgaag gaccggggca cgtcgatggc
     2821 ccggccccgc tcgtgcatcg aggtcccggg ccgagccacc ggataggggt tggtgtggcg
     2881 gcgcgcccag agggcggctt gctgggcccg actccgccag cccgagctga tggggaccgg
     2941 ctcacccagg agggacccgg cccgggcgac ggcggcagcc atctcggagg tca
//
LOCUS       DASTFV010000400         4326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1469194, whole genome shotgun sequence.
ACCESSION   DASTFV010000400 DASTFV010000000
VERSION     DASTFV010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469194"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFP02_07355"
     CDS             <1..185
                     /locus_tag="VFP02_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542881.1"
                     /translation="GFLCRDDPAELAADLTAAMTAAGATCLNLRVHVPGVTPEMAREQ
                     ITRLGTDVLPRLRSDG"
     gene            complement(169..567)
                     /locus_tag="VFP02_07360"
     CDS             complement(169..567)
                     /locus_tag="VFP02_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9542882.1"
                     /translation="MSAPLIVALPTADRPTTYRFYGEGLGFEAIGELADDGVPEPLQF
                     VLNDGARLMFIPTGGFGWVTGDHETAPPGTSECLLSLNVESDAAVDEYIEKARAAGAV
                     VLAEPEQKPWGYLGLFADPDTHLWQVIHPT"
     gene            697..975
                     /locus_tag="VFP02_07365"
     CDS             697..975
                     /locus_tag="VFP02_07365"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HET9542883.1"
                     /translation="MSTIDKQFTATLHKREGKGGWTCVLWPDSVAYFGTKGLVKVRGT
                     VDGEPFQSSFMALGDGTHMLPMKSDVRKAIGKDAGDEVTIVLQERLDA"
     gene            complement(1072..2160)
                     /locus_tag="VFP02_07370"
     CDS             complement(1072..2160)
                     /locus_tag="VFP02_07370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9542884.1"
                     /translation="MDFSFSEEQQAVSDLAAQILGDQSTNEALKAHEASGDAYPAGLW
                     AALADAGLVGIALPEDVGGSGLGFLETCLTLEQIGRTTAPVPYFATVMAALAVNQFGT
                     DEQRSSLLPGVADGSRVLTVALTETGTGPLAPTTEAADGALRGTKLLVPFGQQATHAV
                     VPARVGQDIGLFLVDLAGDGVAQEALVTTSALPATRLDLEGAAGEPLGAADGEALSWL
                     VERATAALCATAIGICEEALRLTAEYTKTREQFDRPIATFQAVGQRAADAYIDTEAVR
                     LTAWQAAWRLSEGLPSTEQVAIAKFWCAEGGQRVVHAAQHLHGGMGVDRDYPLHRYFL
                     WAKELELTLGGATHSLLGLGQILAAEPV"
     gene            complement(2180..3358)
                     /locus_tag="VFP02_07375"
     CDS             complement(2180..3358)
                     /locus_tag="VFP02_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9542885.1"
                     /translation="MYVGYDEDQQALRQELRDYYAELLTPEVQDGLADANGVGPAMRK
                     VVKQMGTDGWLGIGWPEEYGGKNKSQLEQFIFFDESMRAGAPVPMLTINTVGPTIFRY
                     GTDEQKDRFLPQILRGEIHFCIGYTEPGAGTDLAALKTRAVRDGDEYVINGQKIFTSL
                     ASDADYCWLAVRTNPDVSKHKGISMIIVPMDTPGITVQPMDLLSEHDINQVFYEDVRV
                     PAANLVGGENNGWSLITNQLNHERVTLCSSGMLEQTYEEALRYAQETKLADGRRVIDQ
                     EWAQLNLARVRAGLEFLRLINWKVAWGATEGLPLDIGDASTIKVFGTEFYLEAFRLLM
                     EVVGPASYLRRESPEALLRSRLERNYRSLIILTFGGGVNEVQRDLIAMFALGLPRADR
                     "
     gene            complement(3434..>4326)
                     /locus_tag="VFP02_07380"
     CDS             complement(3434..>4326)
                     /locus_tag="VFP02_07380"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9542886.1"
                     /translation="GPSLHALATGLADALAAVHGAGVLHRDVSPANVVLGADGPVLVD
                     FGIARFAEATTLTMTGTVMGTAGWLAPELLRDDDVTAAADIWSWGAVLAFAATGRPPA
                     DGSRAEVVLRKVLDGQLDLRGLPPWLDRLVRRCLDPDPARRPTAAELVAELDPRRALA
                     DAATAPLAATAAAPVEAAPTATLPARPESAPKEQVDWVPLALKAGVVGAGALLGWLAP
                     PLVVMLVIAAAVVLAIVVRVWAEDRAEEKHLPVGPGTIVVAAAVAAITAMAQVIGLLL
                     AIIALVALVVVFFVLGGDFG"
BASE COUNT          637 a         1501 c         1570 g          618 t
ORIGIN      
        1 acggcttcct ctgccgcgac gacccggcgg agctggccgc ggacctgacg gcggcgatga
       61 cggcggccgg cgccacctgc ctcaacctcc gcgtccatgt ccccggcgtc acccccgaga
      121 tggcccggga acagatcacc cgcctcggca ccgacgtcct cccccgcctc aggtcggatg
      181 gatgacctgc cagaggtggg tgtcagggtc ggcgaacagg ccgaggtagc cccaaggctt
      241 ctgctcgggc tcggcgagga cgacggcgcc ggcggcccgg gccttctcga tgtactcgtc
      301 gaccgcggcg tcggactcga cattcaggct taggaggcac tcgctggtgc caggcggggc
      361 ggtctcgtga tcgcccgtca cccagccgaa gccacccgtc gggatgaaca tcagccgggc
      421 cccgtcgttg agcacgaact gcagcggctc ggggaccccg tcatccgcca gttcgccgat
      481 cgcctcgaac cctaaaccct cgccgtagaa gcgataggtc gtcggacggt cggcggtggg
      541 cagggccacg atcaacggtg cgctcatggg tcattggacc ggagcgagca cgcgaactca
      601 tcgttccggt ggcagcggtg ttcccccacc cgccctacgc gatgattctc ggtgcggcgg
      661 cgggtccaat cgatgacgtg acggcaggag gatcccatga gcaccatcga caagcagttc
      721 accgccacgc tgcacaagcg ggagggcaaa ggcggatgga cctgcgtgct ctggcccgat
      781 tcggtggcct acttcggcac caagggcctc gtgaaggtcc ggggcaccgt cgacggtgag
      841 ccgttccaga gctcgttcat ggccctgggc gacggcacgc acatgctccc gatgaagtct
      901 gacgtccgca aagccatcgg caaggacgca ggcgacgagg tgaccatcgt cctccaggag
      961 cgcctcgacg cctgacccga ccgccgacga cgtcgagcgc atcgccagat ctccggctac
     1021 tggcgcctga ttgcggcttc ctggcgccgc aatcgggcgc cagaacgggt gctagacggg
     1081 ttcggcggcg aggatctggc cgaggccgag gaggctgtgg gtggcgccac ccagggtgag
     1141 ctcgagctcc ttggcccaga ggaagtagcg gtgcaggggg tagtcccggt cgacgcccat
     1201 gccgccgtgg aggtgctggg cggcgtggac gacccgctgg ccgccttcgg cgcaccagaa
     1261 cttggcgatg gccacctgct cggtggacgg cagcccctcg ctcaggcgcc aggcggcctg
     1321 ccaggcggtg aggcggacgg cctcggtgtc gatgtaggcg tcggccgccc gttggcccac
     1381 ggcctggaag gtggcgatgg ggcggtcgaa ctgctcgcgg gtcttcgtgt actcggcggt
     1441 gaggcgtagg gcctcctcgc agatgccgat ggccgtcgca cacaaggcag ccgtggctcg
     1501 ctcgaccagc caggacaggg cctcgccgtc agcggcgccg agcggctcgc cggcggcccc
     1561 ctcgaggtcc aggcgcgtgg cgggcagggc cgaggtcgtg accagggcct cctgggccac
     1621 accgtcaccg gcgaggtcga cgaggaacaa accgatgtcc tggcccaccc gggcggggac
     1681 gacggcgtgg gtggcctgct ggccgaaggg cacgagcagc ttggtccccc gcagggcgcc
     1741 gtcggcggcc tcggtggtgg gcgccagcgg accggtgccc gtctcggtca gggccacggt
     1801 cagcacccgg ctgccgtcgg ccacgcccgg cagcagcgac gaacgctgct cgtcggtgcc
     1861 gaactggttc accgccagcg cggccatcac ggtggcgaag tacgggaccg gggccgtcgt
     1921 ccgcccgatc tgttcgaggg tcaggcaggt ctcgaggaag ccgaggccgc tgccgccgac
     1981 gtcctcgggc agggcgatgc cgaccaggcc ggcgtcggcc agggccgccc acagcccggc
     2041 ggggtacgca tcacccgagg cctcatgcgc cttcagggcc tcgttggtcg actggtcgcc
     2101 gaggatctgg gcggccaggt ccgacacggc ctgttgctct tcgctgaacg agaagtccat
     2161 ggctggtgct gtcccccggt tatcggtcgg cccggggcag gcccagggcg aacatggcga
     2221 tcaggtcccg ctgcacctcg ttcacgccgc caccgaaggt gaggatgatg aggctgcggt
     2281 agttgcgctc caggcgggag cggaggagcg cttcgggcga ttcccggcgc aggtaggagg
     2341 ccggtcccac gacctccatg agcagccgga aggcctccag gtagaactcg gtgccgaaga
     2401 ccttgatggt cgaggcgtcg ccgatgtcga ggggcaggcc ctcggtcgcg ccccacgcca
     2461 ccttccagtt gatgaggcgc aggaactcga ggcccgcccg cacccgggcc aggttgagct
     2521 gggcccactc ctggtcgatg acccgacggc cgtcggccag cttggtctcc tgggcgtacc
     2581 gcagggcctc ctcgtaggtc tgctccagca tgccggacga gcacagcgtg acccgctcgt
     2641 ggttcagctg gttggtgatg agcgaccagc cgttgttctc gcccccgacc aggttcgccg
     2701 ccggcacccg cacgtcctcg tagaagacct ggttgatgtc gtgctcgctc agcagatcca
     2761 tgggctggac cgtgatgccg ggcgtgtcca tcgggacgat gatcatcgag atgcccttgt
     2821 gcttggacac gtcggggttc gtccgcaccg ccagccagca gtagtcggcg tcgctggcca
     2881 ggctggtgaa gatcttctgg ccgttgatga cgtactcgtc gccgtcccgc acggctcggg
     2941 tcttgagggc ggccaggtcg gtgccggcgc cgggctcggt gtagccgatg cagaagtgga
     3001 tctcgccccg gaggatctgg gggaggaacc ggtccttctg ctcgtcggtg ccgtagcgga
     3061 agatcgtggg accgaccgtg ttgatggtca gcatcggcac cggggcgccg gcccgcatcg
     3121 actcgtcgaa gaagatgaac tgctcgagct gggacttgtt cttcccgccg tactcctcgg
     3181 gccagccgat gcccagccag ccgtcggtgc ccatctgctt gacgaccttg cgcatggcgg
     3241 ggccgacgcc gttggcgtcg gccaggccgt cctgcacctc aggcgtcaac agctcggcgt
     3301 agtagtcgcg cagctcctgg cgcagcgcct gctggtcctc gtcatagccg acgtacatcc
     3361 gtgtcccccg aagaaagtag aaccggttct agcggcagtc tcgcccacca ctggccgggc
     3421 gctcaactcg cggtcaaccg aagtcgcccc cgagcacgaa gaagacgacg accagcgcga
     3481 ccagggcgat gatggcgagc agcagcccga tcacctgggc catggccgtg atggcggcca
     3541 ccgcagccgc cacgacgatg gtccccggtc cgaccggaag gtgcttctcc tcggcccggt
     3601 cctcggccca cacccggacg acgatggcca ggacgacggc cgccgcgatg acgagcatca
     3661 cgacgagggg cggggccagc cagcccagca gcgcgccggc cccgaccacc cccgccttca
     3721 gggcgagcgg gacccagtcc acctgctcct tcggtgccga ctcgggccgc gccggcagcg
     3781 ttgcggtcgg cgccgcctcg acgggggcgg cggcggtggc ggccaacggg gcggtggcgg
     3841 catcggccag ggcccgccgc gggtcgagct cggcgaccag ctcggccgcc gtcggacggc
     3901 gggccgggtc cggatccagg cagcggcgca cgagccggtc caaccacggg ggtaggcccc
     3961 ggagatcgag ctggccgtcc aggaccttgc gcagcacgac ctcggcccgg ctgccgtcgg
     4021 ccggtgggcg cccggtggcg gcgaaggcca gcaccgcccc ccaggaccag atgtccgccg
     4081 ccgccgtcac gtcgtcgtcc cggagcagct ccggggccag ccagcctgcg gtgcccatga
     4141 ccgtgcccgt catcgtcagg gtggtggcct cggcgaatcg ggcgatgccg aagtcgacga
     4201 gcacgggccc gtccgcaccg aggacgacgt tggcggggct cacgtcgcgg tgcaggacac
     4261 cggcgccgtg gaccgcggcg agcgcgtcgg cgaggcccgt ggccagagcg tgcagggagg
     4321 gtcccg
//
LOCUS       DASTFV010000401         3554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1469960, whole genome shotgun sequence.
ACCESSION   DASTFV010000401 DASTFV010000000
VERSION     DASTFV010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469960"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1768
                     /locus_tag="VFP02_07385"
     CDS             245..1768
                     /locus_tag="VFP02_07385"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET9542887.1"
                     /translation="MSKRSIGSVLVGALAVLGLIAPSADAQRPPRPPSVDADGVTEHL
                     EALQEVADDNGDTRASGTSGYEGSVDYVVERLRAAGYRPEVQEFTFPFFQQLGPTTFA
                     EVSPTAQTFVEGTDYFLGEYSGSDDVSAPLQAVDLVLPPTPTPSSTSGCEAADFAGFT
                     PGNIALIQRGTCTFEQKVANAQDAQAAGVVLFNEGQPGREDAFTPALGRPFTVPVVMA
                     SFAVGNALFAELTPGPVEIHLATDTFSENRQTWNVLADTRGGNSHHTVVVGGHLDSVL
                     AGPGINDNGSGSATILEVAEALGSGDNGPDGRGLTNRIRFAFWGAEENNLLGSEHYVA
                     NLTESELGDIALNLNFDMLASPNFVRFVYDGDGSDTATAGPDGSDAIEQSFLQYFDSQ
                     GLATDPTAFDGRSDYGPFIAVGIPAGGLFSGAEGEKTPEQAAVYGGTAGEPYDSCYHA
                     ACDTTANVNDEVLEQMADAVAYTTWYWGTHRLPPRPATEALTTARLVGGGTGHSVTA"
     gene            1864..2628
                     /locus_tag="VFP02_07390"
     CDS             1864..2628
                     /locus_tag="VFP02_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9542888.1"
                     /translation="MDISGASALVTGGASGLGGAISRLLAELGAKVTVVDLQDEKGEA
                     MAAEIGGKYAHADVTNTEEVEAAVATANADGDLRMVANCAGIGWAQRTVGKGGEPHDL
                     AVFEKVVQVNVIGTFNVIRLAASAMSQNDPLGEDGERGAMVNIASVAAFDGQTGQASY
                     SAAKGGIVGMTLPIARDLAAVGIRVCTIAPGTIDTPMFAFASEDTKAGLGAGVLFPKR
                     MGRPEEVASLAAEIFRNSYFNAETIRLDGGIRMPPK"
     gene            complement(2630..3232)
                     /locus_tag="VFP02_07395"
     CDS             complement(2630..3232)
                     /locus_tag="VFP02_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HET9542889.1"
                     /translation="MSEARGRLVAAFDFDGTITRKDTLVPFLRRFRGLPRFAGAFVQT
                     LPAALTGPDRREAYKAAVFHQLLRGVPAEDLTAAALDYGRQLPAQFRPATVERIRWHQ
                     EEGHEVVIVSASLRAYLDPVVEHLGLDGVCAVELEVADDGRLTGRMAGPNCRGAEKVV
                     RLTDWLGGDSPERLWAYGNSSGDRELLAAAHEPVWIGRRR"
     gene            complement(3225..>3554)
                     /locus_tag="VFP02_07400"
     CDS             complement(3225..>3554)
                     /locus_tag="VFP02_07400"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9542890.1"
                     /translation="GSVKGATGDPEPLVLGSRFGMNMRIGVPYRIQNEVVEYEENRLI
                     AWQHFGKHRWRYEFEPVDGGTKVTETFDWSTSRSPRVIEFMGYPKKHPDNMAKTLERL
                     ETVVAGE"
BASE COUNT          555 a         1271 c         1214 g          514 t
ORIGIN      
        1 cccaatccat tccgtcatct ttcagatgac ctgtgacatt ttcgttctgg tgactgaggt
       61 ttgccgaggg aacaagcccg agtcaccaga acgaatggcg tgcggtcctg gtgattgagg
      121 tttgttgggg ggacaagtcc gagtcaccag aacgaacggc ccctggggtg gccgggggca
      181 tgacgggggt gagggcgggg taggacgggg gcggcgcggg cccgtcttcg gaaagagatc
      241 cttcatgtcg aaacgttcca tcgggagcgt gctggtcggc gcgctcgcgg tgctggggct
      301 gatcgccccg agcgccgatg cccaacgtcc gccccgcccc ccctccgtcg acgccgacgg
      361 cgtgaccgaa cacctcgaag cgctccagga agtcgccgac gacaacggcg acacccgggc
      421 ctccggtacg tcgggctacg aggggtccgt cgactacgtc gtcgagcgcc tgcgcgccgc
      481 cggctaccgc ccggaggtgc aggagttcac cttcccgttc ttccagcagc tcgggcccac
      541 cacgttcgcg gaggtcagcc ccacggcgca gaccttcgtc gaggggaccg attacttcct
      601 cggcgagtac tccgggtccg acgacgtgtc ggcgccactg caggccgtcg acctcgtgct
      661 cccgcccaca ccgacgccga gcagcacctc cggctgtgag gccgcggact tcgccggctt
      721 caccccgggc aacatcgctc tgatccagcg gggtacctgc accttcgagc agaaggtcgc
      781 caatgcccag gacgcccaag ccgccggtgt cgtcctcttc aacgagggcc agcccggccg
      841 ggaagacgca ttcaccccgg ctctcgggcg gccgttcacc gtgccggtcg tcatggccag
      901 cttcgcggtg ggcaatgccc tcttcgccga gctgaccccc ggcccggtcg agatccacct
      961 ggccacggac accttctccg agaaccggca gacctggaac gtcctggccg acacccgtgg
     1021 gggcaactcg caccacacgg tcgtggtcgg tggccacctc gacagcgtgc tggccggtcc
     1081 cggcatcaac gacaacggtt ccgggtcggc caccatcctc gaggtcgccg aggcgctggg
     1141 gagtggcgac aacggccccg atgggcgagg gctcaccaac cgcatccgct tcgccttctg
     1201 gggtgcggag gagaacaacc tcctcgggtc ggagcactac gtggcgaacc tcaccgagag
     1261 cgagctgggt gacatcgccc tcaacctgaa cttcgacatg ctcgcctcgc cgaacttcgt
     1321 ccgcttcgtc tacgacggcg acggcagcga caccgccacc gccggaccgg acggctccga
     1381 cgccatcgag cagtcgttcc tgcagtactt cgacagccag ggtctggcga ccgacccgac
     1441 ggccttcgac ggacgcagcg actacggccc gttcatcgcc gtcggcatcc ccgccggtgg
     1501 cctgttcagc ggcgccgagg gcgagaagac cccggagcag gctgccgtct acggcggcac
     1561 agccggtgag ccgtacgact cctgctacca cgcggcgtgc gacaccactg ccaacgtcaa
     1621 cgacgaggtg ttggagcaga tggccgacgc cgtcgcctac accacctggt actggggcac
     1681 gcaccgcctg ccgccccggc ccgccacaga ggcgttgacg actgcccgcc tcgtcggcgg
     1741 cggcaccggt cactccgtga ccgcctgagg gatcggcccc gctcgagccc agtgctcggg
     1801 cggggccgca ggcccccgcg ccgggttcgc gagccgaagg gggcgtcact aacgtcgctg
     1861 ggcatggaca tcagcggagc atcagcactg gtcaccggcg gggcctcggg cttgggtggg
     1921 gccatctccc gcctgctggc cgagctcggt gccaaggtca ccgtcgtcga cctccaggac
     1981 gagaagggcg aggccatggc ggccgagatc ggcgggaagt acgcccacgc cgacgtgacc
     2041 aacaccgagg aggtcgaggc cgcggtggcc acggcgaacg ccgacggcga cctgcgaatg
     2101 gtggccaact gcgcgggcat cggctgggcc cagcgcacgg tcggcaaggg cggtgagccc
     2161 cacgacctcg cggtcttcga gaaggtcgtc caggtcaacg tgatcggtac gttcaacgtc
     2221 atccgcctcg cggcgtcggc catgagccag aacgatccgc tgggggagga cggcgagcgg
     2281 ggagcgatgg tcaacatcgc ctcggtcgcg gccttcgacg gccagaccgg ccaggccagc
     2341 tactccgccg ccaagggtgg catcgtgggc atgaccctgc ccatcgcccg ggacctggcc
     2401 gcggtcggca tccgggtctg caccatcgcc ccgggcacga tcgacacgcc gatgttcgcg
     2461 ttcgcctccg aggacacgaa ggcgggcctg ggggccggcg tgctcttccc caagcgcatg
     2521 ggtcggcccg aggaagtggc ctcgctcgcc gccgagatct tccgcaacag ctacttcaac
     2581 gccgagacca ttcgcctcga cggtggcatc cggatgcctc ccaagtagtt cagcgccgcc
     2641 ggccgatcca gacgggctcg tgggcggcgg ccagcagctc gcggtcgccc gaggagttgc
     2701 cgtaggccca caggcgctcc gggctgtcgc ccccgagcca gtcggtgaga cggacgacct
     2761 tctccgcccc gcgacagttg gggccggcca tgcgcccggt caggcgaccg tcgtcggcca
     2821 cctccaactc gacggcgcag acgccgtcta ggccgaggtg ctcgaccacg gggtcgaggt
     2881 aggcccgcag cgacgcggag acgatcacca cctcgtggcc ctcttcctgg tgccagcgga
     2941 tgcgctcgac cgtcgccggg cggaactggg ccggcagctg ccgcccgtag tcgagggccg
     3001 cggccgtcag gtcctcggcg ggcacgcccc tgagcagctg gtggaacacc gccgccttgt
     3061 acgcctcccg tcggtcgggc ccggtcaggg ccgccgggag ggtctggacg aaggccccgg
     3121 cgaagcgggg caggccgcgg aaccggcgca ggaacgggac cagcgtgtcc ttgcgggtga
     3181 tggtgccgtc gaagtcgaat gcggccacca gccgcccgcg ggcctcactc accggcgacg
     3241 acggtctcca agcgctcgag cgtcttggcc atgttgtccg gatgcttctt cgggtacccc
     3301 atgaactcga tgacccgcgg tgagcgggag gtcgaccagt cgaaggtctc ggtcaccttg
     3361 gtcccgccat cgaccggctc gaactcgtag cgccaacggt gcttgccgaa gtgctgccag
     3421 gcgatcaggc ggttctcctc gtactccacc acctcgttct ggatccggta gggcaccccg
     3481 atgcgcatgt tcatgccgaa gcgggaaccg aggacgagcg gctcggggtc accggtcgcc
     3541 cccttgaccg agcc
//
LOCUS       DASTFV010000402         4070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1483707, whole genome shotgun sequence.
ACCESSION   DASTFV010000402 DASTFV010000000
VERSION     DASTFV010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483707"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFP02_07405"
     CDS             complement(<1..199)
                     /locus_tag="VFP02_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542891.1"
                     /translation="MDDGSGPPEEWLARRLAARSGQRRSAVAAALHELAETDRSVYRA
                     IASTPSPTLDAPLRRLSNVANQ"
     gene            complement(239..2854)
                     /locus_tag="VFP02_07410"
     CDS             complement(239..2854)
                     /locus_tag="VFP02_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9542892.1"
                     /translation="MTMTGRAAASLPAPPPDFVVNRPALVDRLESATQKRVVRIVAPA
                     GYGKSVLLAQWQRAHPERRTAWVEARPTDDAARFGRRLAEALEPVVPAMAQRAFAHIR
                     RDGVALGEDFLAIVLAELSYTAPVTVVVEDLETLDSPLVLDELGLLAERAADGVGFVF
                     VSRDDRLPNTLRLRLRDEVAEIRQDTLALSWEETGEAIRRVTGQTLHPAQVQALHSRT
                     EGWAAGIQLAALSLRDHDDPDRFVEQFAGDDRHVADYLSGEVLALQPPEVTDFLVQVA
                     VLDRLSGSLCDAVTGGTDGQRMLERLDEQSLFIRPLDGRRQWYGYHPLFRDLLRYELR
                     ATRPGLEVDLLRRAAAWHLQQGETDEAAEYLLRASDWAAVIALARAEGGRYFERGEAT
                     SVLRWLGAVPSDVLLADPDAVLVKVTLHTTCGSSMVAQQLLDRLESSVTLDVGQQALA
                     ATIRACWIYHHLPPDVAAAAAETALRLLDGGSDLSGTVLLDVLTPAALRSLATICQAV
                     ACWSVADHQGARALLATVVDQPGRVVWLVDALGEVAWVAVATGHLRLGLATARRALDL
                     AEEAGLGDHTGTAMAHFALARAHRMRGEAVAGHLDTALAQARLNQRTRVLSLEWAERA
                     DAALGDGRASEGLDQIAAAQVAGGPPLAPIVEADLVALEIRLHLLAGNPSSAGAALER
                     HGGIRTASLASAAAGLAIATDDQPTLRKLVEDWDVVDRDEPVSRWGRGLCAAVLADRE
                     GDRRAALAAVTEVVAEAEAEGALRPFLDGGPEVLRLVRALYHADPTPFLRRLVEDVPP
                     VSPRGSTEMVEQLTERELLVLRYLPSRLSNADIAARLYVSINTLKTHIKNIYRKLEVG
                     DRGEAIARAEELGLL"
     gene            complement(2941..3159)
                     /locus_tag="VFP02_07415"
     CDS             complement(2941..3159)
                     /locus_tag="VFP02_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542893.1"
                     /translation="MSTGSHYELVVTGRPGPRVLAALPGFEVLASDGDQTCLRGWVQD
                     QAALQGLMRSLGDLGVAIEGLQRVAPEN"
     gene            3309..3755
                     /locus_tag="VFP02_07420"
     CDS             3309..3755
                     /locus_tag="VFP02_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HET9542894.1"
                     /translation="MSDDFEEMGPIDWLLVEFSGPPTGAAAPYLLDLVERGLIRILDL
                     VVVNKDADGTITMLELADVDGDGELDLAVFEGAQSGLLGEEDVAEAGEAMEAGTSGVF
                     LLYENVWAAPFAVAVRKAGGQLVASGRIPVQSIIAALDDLEAADQS"
     gene            3766..4047
                     /locus_tag="VFP02_07425"
     CDS             3766..4047
                     /locus_tag="VFP02_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HET9542895.1"
                     /translation="MMRRRGGLVRGVARTAVVAGTATAVSGRVRRRQDAKWSEQEQAE
                     YEQQMAQQPAPAPAPAGDAAELQQLADLHAQGILTDEEFAAKKAQILGI"
BASE COUNT          623 a         1427 c         1485 g          535 t
ORIGIN      
        1 attggttcgc tacgttcgag agcctgcgca ggggagcatc cagggtgggg gacggggtcg
       61 acgcgatcgc ccggtagacg gaccggtcgg tctccgccag ttcgtggagc gcggcggcga
      121 cggccgaacg gcgctggcct gaccgggccg cgaggcgccg ggccagccat tcctccgggg
      181 gaccggaacc gtcgtccacg ccccgatgat gccccgccat gtgtcggggc gctgccgcct
      241 agaggaggcc gagctcctct gcccgagcta ttgcctcacc gcggtcgccg acctcgagct
      301 tccggtagat gttcttgatg tgggtcttga gcgtgttgat cgagacgtag agccgggcgg
      361 cgatgtcggc gttggacagt cggctcggca ggtagcggag caccaggagc tctcgctcgg
      421 tgagctgctc gaccatctcg gtggacccgc gcgggctcac gggcggcaca tcctccacca
      481 gtcgtcgcag gaacggcgtg ggatcggcgt ggtacagcgc ccgcaccagg cgcaggacct
      541 cgggaccgcc gtcgagaaac gggcgcaggg ccccctcggc ctcggcctcg gcgacgacct
      601 cggtcacggc ggccagggcc gcgcgccggt ccccttcgcg atcggccagc acggcggcac
      661 agagcccccg accccagcgg gagaccggct cgtcgcggtc gacgacgtcc cagtcctcga
      721 cgagcttgcg cagggtgggc tggtcgtcgg tggctatggc caggccggcg gcagccgagg
      781 ccaatgaggc cgtgcggatc ccgccatgac gctccagggc ggccccggcc gaggaggggt
      841 tcccggcgag gaggtggaga cggatctcca gggcgaccag gtcggcctcg acgatcggag
      901 ccagcggcgg gccgccggcc acctgggccg cggcgatctg atccagtccc tcgctggccc
      961 gaccatcgcc gagggcggca tcggctcgtt cggcccactc cagggagagc acccgggtcc
     1021 gctggttcag gcgggcctgg gccagtgccg tgtcgaggtg cccggccacg gcttcccccc
     1081 gcatccggtg ggcccgggcc aaggcgaagt gggccatcgc cgtccccgtg tggtcaccga
     1141 gcccggcctc ctcggccaga tccagtgctc gccgagcggt agccagcccg aggcgcaggt
     1201 gaccggtcgc caccgcgacc caggccacct caccgagggc gtcgaccaac cagaccaccc
     1261 gacccggctg gtcgaccacc gtcgccaaga gggcccgcgc cccctgatga tcggccacgg
     1321 accaacaggc cacggcctgg cagatggtgg cgagggaccg cagcgcggcg ggggtcagga
     1381 cgtcgaggag gacggtgccg gagaggtccg aacctccgtc gagcagtcgg agggctgtct
     1441 cagccgcggc ggcggccacg tcgggcggga ggtggtggta gatccagcag gcccggatgg
     1501 tggccgccaa cgcctgctgg ccgacatcga gggtgaccga gctctcgagc cggtcgagga
     1561 gctgctgggc gaccatggac gagccacacg tcgtgtgcag cgtcaccttc accagtacgg
     1621 catcggggtc ggccaggagc acgtccgacg gcaccgcccc caaccagcgg agcacgctcg
     1681 tcgcttcacc tcgctcgaag tagcggccgc cttccgctcg ggccagggcg atgaccgcgg
     1741 cccagtcgct cgcccgaagg aggtactcgg cggcttcgtc ggtctcgccc tgctgcaggt
     1801 gccacgccgc cgcccggcgg agcagatcca cctccaggcc gggtcgggtc gcccgcagtt
     1861 cgtaccggag caggtcccgg aacagggggt ggtagccgta ccactgtcgg cgcccgtcca
     1921 ggggccgtat gaagagtgac tgctcgtcga ggcgctcgag catccgctgc ccgtcggtac
     1981 caccggtgac cgcatcacac agggacccgc tgagccggtc gagcacggcg acctgcacga
     2041 ggaaatccgt gacctccggc ggctggaggg cgaggacctc gccactgagg taatcggcca
     2101 catggcggtc gtcgccggcg aactgctcca cgaagcggtc ggggtcgtcg tggtcgcgca
     2161 ggctcagcgc ggccagctgg atccccgcgg cccagccctc ggtccgcgag tggagggcct
     2221 ggacctgcgc agggtgcagt gtctggccgg tgacccgtcg gatggcctcg cccgtctcct
     2281 cccaagagag ggccaaggtg tcctggcgga tctcggccac ctcgtcccgg aggcgcagtc
     2341 ggagcgtgtt ggggagccgg tcgtctcggc tgacgaagac gaagcccacg ccgtcggccg
     2401 cccgttcggc gagcaggccg agctcgtcga gcaccagggg gctgtccaac gtctcgaggt
     2461 cctcgacgac caccgtgacc ggagcggtgt acgacagctc ggcgaggacg atggccagga
     2521 agtcctcacc gagggcgacg ccgtcccggc ggatgtgggc gaaggcccgc tgcgccatgg
     2581 ccggcacgac gggctcgaga gcctcggcca gccggcgacc gaaccgggcg gcgtcgtcgg
     2641 tggggcgggc ctccacccac gccgtccgcc gctccgggtg cgcgcgctgc cactgggcca
     2701 ggaggacgga cttgccgtag ccggcggggg ccacgatgcg caccacccgc ttctgggtcg
     2761 ccgattcgag gcgatccacg agggccgggc ggttcacgac gaaatcgggc ggaggcgcgg
     2821 gaagcgaggc tgcagcccgc cccgtcatgg tcatctcacg tcaccttccc agtcccaagg
     2881 tctggaggcc ggaacacaaa cgctagacct ccgccaaccc cccgtcgaat gacgtgacct
     2941 tcaattctcc ggagcgaccc tctgcaggcc ctctatggcc actcccaggt cgcccagcga
     3001 ccgcatgagg ccctgtagtg ccgcctggtc ttggacccag ccgcggaggc aggtctggtc
     3061 gccgtcactg gccagcacct cgaacccggg cagggcggcg agcacgcgcg gcccggggcg
     3121 gccggtgacc acgagctcgt agtgggaccc cgtggacacg tcttcactct ggaccctccg
     3181 gatcgccgcc gagtcacccg cgaggaatga aatgggagaa gttcacccgg ccgaggtgat
     3241 ggcggttctc gagttcagcg gctagaacga cagcacgcgg agggcaacgt gcccacaggg
     3301 aggacgaggt gagcgacgac ttcgaggaaa tgggcccgat cgactggctg ctggtcgagt
     3361 tctcgggacc gccgaccggc gccgccgcgc cttacctctt ggacctcgtc gagcggggac
     3421 tgatccgcat cctcgacctg gtcgtggtga acaaggacgc cgacggcacc atcacgatgc
     3481 tggagctggc cgacgtcgac ggcgacggcg agctcgacct cgcggtcttc gagggcgccc
     3541 aatccggcct cctgggcgag gaggacgtgg ccgaggccgg cgaggccatg gaagcgggca
     3601 cctccggggt cttcctgctc tacgagaacg tctgggccgc gccgttcgcc gtcgccgtcc
     3661 gcaaggcggg gggccagctc gtggcctccg gccgcattcc cgtccagtcc atcatcgctg
     3721 ccctcgacga tctcgaagca gcagaccaga gctgaggaga gcaccatgat gcgtcgaaga
     3781 ggaggcctcg tccgaggcgt cgcccggacc gcggtcgtcg ccggaacggc cacggcggta
     3841 tccggacgtg tccggcgtcg ccaagacgcc aagtggtccg agcaggagca ggccgagtac
     3901 gagcagcaga tggcgcaaca gccggctccg gcaccagcgc ccgcgggtga cgcggccgag
     3961 ctgcagcagc tggccgacct gcacgcccag ggcatcctca ccgacgagga gttcgcggcc
     4021 aagaaggcac agatcctcgg gatctgaccg ccgtccggtg agcgccggcg
//
LOCUS       DASTFV010000403         3705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1485659, whole genome shotgun sequence.
ACCESSION   DASTFV010000403 DASTFV010000000
VERSION     DASTFV010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485659"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VFP02_07430"
     CDS             <1..909
                     /locus_tag="VFP02_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542896.1"
                     /translation="PLPDAVAARVEAFADALGVGDDCREVVAATRHLAAGATDMALAD
                     FMRNGYEVLHLERAGEARPEDVSSYWAPAHDDPALAARWDAMQDGSPTSLGRCVWEFY
                     KGRGFNFPGTPNSVPPRLAQHDFVHVLADYGTTVESEIEVFALIARADEDPRSFSLLV
                     AVLALFEGGYLEQGLGAFRADPGHLSHDSSGMGIRLGNAIARGGEVAWRFHENRGIDL
                     LAVDWHDRAHEELDDLRQEFGFDGPGAKSLEAKAAGSAGPFQPGGVTVAQLQWGRDMA
                     RAEGRAYDPFGASLSTGQADPPDGAD"
     gene            909..2093
                     /locus_tag="VFP02_07435"
     CDS             909..2093
                     /locus_tag="VFP02_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542897.1"
                     /translation="MPVPFPSDADVNLDPPEPPEILHVARGVMTAVAPPEGLTRMQTL
                     CFRSLSDAMTGHPLDLAAVTPISAEDYARGLARRDRAFRIRMVQMMIVLALLLRRVPP
                     EVAARLQAFADELGVGEDCRDLLNATRHLAAGALGLAMADFERNGYETMAQERSGDGA
                     EHTAAEYWAEAPDDPELAARWQGLEQCAEGTLGRRMWEFYRARGFAFPGTPGSAPPAL
                     AQHDWIHVLGDYGTTVESELEVFGLIARASDDPRAFTLLIQVLGLFESGYLQAGMGLF
                     QMDVGHLSADEEAMALRLADAFRRGARAAWSYNDANDTDTGIDFLAEDWFAHADQTLD
                     GVQATFFLDGPAAKSDEAVAVGSVGPWEAGGISPYQAQAGRDRAEAEGRPYDAYGATP
                     AP"
     gene            2103..2906
                     /gene="murI"
                     /locus_tag="VFP02_07440"
     CDS             2103..2906
                     /gene="murI"
                     /locus_tag="VFP02_07440"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET9542898.1"
                     /translation="MQAPIGMFDSGFGGLTVARATIDLLPAEDLVYVGDTGRYPYGPR
                     AQDEVAGFAEQITKYLVAEHDVKLVVVACNTAAAAALAQLQADTPVPVIGVIEPGVRS
                     LVSATRNGRVGVIGTVGTIRSGAYQRAVADTGATVDPVFAACPGFVEFVERGEADSDQ
                     VHVLAERLLAPVRERGVDTLLLGCTHYPYLARTLSDVMGREVVLVSSADETAFAVSRA
                     LADLDLRRDDPDRVGSHRFVSSGDVGSFARLGQRLLGPELVDAEGVTWG"
     gene            2897..3634
                     /locus_tag="VFP02_07445"
     CDS             2897..3634
                     /locus_tag="VFP02_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9542899.1"
                     /translation="MGLSLTVLGCGGTYAGPDNACSGYLLRTATTTVLVDLGSGSLAN
                     AQRHIDLTDVDAFVLTHEHPDHWLDLPVARNVYRYVLDRTGVPVFTTAGTQALAVPFC
                     RDTTFAWTTIADGDRATIGDIAFGFSRTDHPVETLAVLAEADGASVVYSSDTGPGWSP
                     LAFGIRPDLALLEATFGGGHSRPVHLTAAEAGERAAAVGARQLVLTHLLPGTDPARQK
                     AEAEEAYAGPVTVAEPGVSFAVEAERR"
BASE COUNT          474 a         1384 c         1317 g          530 t
ORIGIN      
        1 ccgctgcccg acgcggtcgc ggcccgggtg gaggcctttg ccgacgcgtt gggcgtgggc
       61 gacgactgcc gggaggtggt ggccgcgacc cgccacctgg cggcgggcgc caccgacatg
      121 gccctggccg acttcatgcg caacggctac gaggtgctgc atctcgaacg ggcgggcgag
      181 gcgcgcccgg aggacgtcag ttcctactgg gccccggccc atgacgaccc tgcgttggcg
      241 gcccgctggg acgccatgca ggatggctca cccacctcgc tgggccggtg cgtctgggag
      301 ttctacaagg gccgcggctt caacttcccc gggacgccga acagcgtccc gccccgcctg
      361 gcccagcacg acttcgtcca cgtgctcgcc gactacggca cgacggtgga gtccgagatc
      421 gaggtgttcg ccctgatcgc ccgggccgac gaggacccgc gctcgttcag cctcctggtc
      481 gccgtcctcg cgctgttcga gggtggctac ctggagcagg gcctgggtgc gttccgggcc
      541 gacccgggcc acctcagcca cgactcgtcg ggcatgggca tccggctcgg caacgccatc
      601 gcccggggtg gcgaagtggc gtggcggttc cacgagaacc gaggcatcga cctcctggcc
      661 gtcgactggc acgaccgggc ccatgaggag ctcgacgacc tgcgtcagga gttcggcttc
      721 gacggacccg gggccaagtc cctggaggcc aaagcagccg gttcggccgg cccgttccaa
      781 cccggcggcg tcaccgtcgc ccagctccag tggggccggg acatggcccg ggccgaggga
      841 cgggcctacg accccttcgg ggcgtcgctg tcgaccggtc aggcggatcc gcccgacggc
      901 gcagactgat gccggtcccg ttcccctccg acgccgacgt caacctcgat ccccccgagc
      961 cgcccgagat cctccacgtc gcccgcgggg tcatgaccgc ggtcgcccct cccgagggcc
     1021 tcacccgcat gcagacgctc tgcttccggt cgctgtccga cgcgatgacg ggccatccgc
     1081 tggacctcgc cgcggtcacg cccatctcgg ccgaggacta cgcccgtggc ctggcccgac
     1141 gggaccgggc gttccggatc cgcatggtcc agatgatgat cgtcctggcg ctgctcctgc
     1201 gtcgggtgcc gcccgaggtg gccgcccgcc tccaggcgtt cgccgacgag ctgggcgtcg
     1261 gcgaggactg ccgggacctg ttgaacgcca cccgccacct ggcggccggc gcgctcggcc
     1321 tggccatggc cgacttcgag cgcaacggct acgagacgat ggcccaggag cgctcggggg
     1381 acggggccga gcacaccgcg gccgagtact gggccgaggc tcccgacgac ccggagttgg
     1441 cggcgcggtg gcagggcctc gagcagtgcg ccgagggcac gctcggccgg cggatgtggg
     1501 agttctaccg ggcccggggc ttcgccttcc cgggcacacc gggcagcgcc ccgccggcgc
     1561 tggcccagca cgactggatc cacgtcctcg gcgactacgg cacgacggtc gagtccgagc
     1621 tcgaggtgtt cggtctcatc gcccgggcgt ccgacgatcc ccgggccttc accctgctca
     1681 tccaggtgct gggactcttc gagtccggct acctgcaggc gggcatgggg ctgttccaga
     1741 tggacgtcgg ccacctgtcg gccgacgagg aggcgatggc gcttcggctc gccgatgcct
     1801 tccgccgggg ggcccgagcg gcgtggtcct acaacgacgc caacgacacg gacaccggca
     1861 tcgacttcct ggccgaggac tggttcgccc acgccgacca gaccctcgac ggggtccagg
     1921 ccaccttctt cctcgacggt ccggcggcca agtccgacga ggcggtggcc gtcgggtcgg
     1981 tcgggccgtg ggaggcgggt ggcatctccc cgtaccaggc ccaggcgggg cgggaccggg
     2041 ccgaggccga aggtcgaccg tacgacgcct acggggcgac accggctccc taggctgcgg
     2101 cggtgcaggc tccgatcggg atgttcgaca gcggcttcgg tggcctgacc gtcgcccggg
     2161 cgacgatcga cctcctgccc gccgaggacc tcgtctacgt cggcgacacc gggcgctatc
     2221 cctacgggcc gcgagcccag gacgaagtgg cgggcttcgc cgagcagatc accaagtacc
     2281 tggtggccga gcacgacgtg aagctggtcg tggtggcctg caacaccgcg gccgccgccg
     2341 cgctggccca gctccaggcc gacaccccgg taccggtgat cggcgtgatc gagcccggcg
     2401 tccggtccct ggtgagtgcc acccggaacg ggcgcgtggg cgtgatcggc acggtgggca
     2461 cgatccgctc gggggcgtat cagcgggccg tggccgacac gggggccacg gtcgatccgg
     2521 tgttcgccgc ttgccccggc ttcgtcgagt tcgtggagcg gggggaggcc gacagcgacc
     2581 aggtccatgt cctggccgag cgcctcctcg ctccggtccg ggagcgcggc gtcgacacgc
     2641 tcctgctcgg ctgcacccat tacccgtacc tggctcggac cctgtccgat gtgatgggcc
     2701 gggaggtcgt cctcgtctcc tcggctgacg agacggcctt cgccgtgtcc cgggccttgg
     2761 cggatctgga cctccggcga gacgatccgg accgggtcgg atcgcaccgg ttcgtgtcgt
     2821 caggggacgt gggctccttt gcccggctcg gccagcgcct gttgggcccc gaactggtcg
     2881 acgccgaggg cgtgacgtgg ggctgagcct gaccgttctc ggctgcggcg ggacttacgc
     2941 cgggccggac aacgcctgca gcggctacct gctgcggacg gccacgacga ccgtcctggt
     3001 cgacctcggc tccggctcgc tggccaacgc ccagcgtcac atcgacctga ccgatgtcga
     3061 cgcgttcgtc ctgacccacg agcacccgga ccactggctg gacctgcccg tggcccgcaa
     3121 cgtctaccgg tacgtgctcg accgcacggg tgtgccggtc ttcacgacgg ccggtaccca
     3181 ggccctggcc gttccgttct gtcgggacac caccttcgcc tggacgacca tcgccgacgg
     3241 cgatcgggcc acgatcggcg acatcgcctt cggcttcagt cgcaccgacc atccggtcga
     3301 gaccctggcc gtcctcgccg aggccgatgg ggcctccgtc gtctactcct cggacaccgg
     3361 tcccggttgg tcgccgctgg ccttcggcat ccggcccgac ctcgccctgc tcgaggccac
     3421 cttcgggggc gggcacagcc gccccgtcca cctgacagcc gccgaggcgg gggagcgggc
     3481 ggctgccgtc ggggcccgcc agctggtgct cacccacctc ctgcccggaa cggacccggc
     3541 ccgccagaag gccgaggccg aggaggccta cgccggcccg gtcaccgtgg ccgagccggg
     3601 cgtcagcttc gcggtcgagg ccgagcggcg atagacacag cggccatgag caacccctcg
     3661 ccttcgcccc gcgccgacgg ccgcctcccc gacgagctcc gcccg
//
LOCUS       DASTFV010000404         7037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1488477, whole genome shotgun sequence.
ACCESSION   DASTFV010000404 DASTFV010000000
VERSION     DASTFV010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488477"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VFP02_07450"
     CDS             complement(<1..262)
                     /locus_tag="VFP02_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542900.1"
                     /translation="MIDRITTTRARNHFVGVALVAIVAGFLAPLAASPAGAAPVPAVT
                     YSAGDIDGQNGWAGTSGANINDSLDQEVVVNSGAPASFRNQSW"
     gene            complement(449..844)
                     /locus_tag="VFP02_07455"
     CDS             complement(449..844)
                     /locus_tag="VFP02_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HET9542901.1"
                     /translation="MSPDRRSVLKGLGAAAVVVGGGVVGWRLLDDDDAPAAPATTEPP
                     PEPTSSLAEALAAVGGRYLEVAPDEADEQVLLAALPALDGTVPERPGQDLRVLAPQAS
                     EDHATGEVIVLDGWVLSLTEARASALYAL"
     gene            complement(841..2430)
                     /locus_tag="VFP02_07460"
     CDS             complement(841..2430)
                     /locus_tag="VFP02_07460"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET9542902.1"
                     /translation="MILDAEDLDSGSRLTADVCILGAGAAGITLARDLEGTGLQVVVL
                     ESGAEALEPDIQDLARGDSVGETLGFSDNPATLDSIRLRQLGGTTNHWAGFCRPYDEV
                     DFEVRPALERSGWPITRADIDPWYQAAHPVLGLGPYRFDWEYWVDELGGGEPLIDTPL
                     VQTGVYQIRDARFGPMYRDELDAATDVTVYLRANAVDLRLDDAGDRVVEVRAKTLGGV
                     EFGVVEPQVVVVALGGIENPRLLLASNSQRPAGLGNEADLVGRHFCEHFAVPAAIAVT
                     GNDPAALSALFEADQGAEEGTLRIKGDVALTSAAVRDRGLLSLGCQLVVGAYAEERPR
                     AISGLGISQVAAVAQATGTDAPRSSLYFLATAEQELNPDSRVRLGSERDPLGMPRVEL
                     DWQFTERDRSSILEGMAVIGDELARTGLGRLQLTPGTITFERTDGIRPYVGGLAIDAD
                     AADPEGFELGLGFHHMCTTRMAADPAEGVVDADLRVHTVDNLYVTGSSSFGTAGASTP
                     TYTLVALTLRLADHLQREVLV"
     gene            complement(2437..2724)
                     /locus_tag="VFP02_07465"
     CDS             complement(2437..2724)
                     /locus_tag="VFP02_07465"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET9542903.1"
                     /translation="MPDRLTDDELAEALTTLPNWSVVDGKLHRDIVLGDFAEAFGLMA
                     HVAVWAEHLNHHPEWSNVYNRVTIDLMTHDAGGLTELDVSLATRVDRALGE"
     gene            complement(2734..3528)
                     /gene="sufC"
                     /locus_tag="VFP02_07470"
     CDS             complement(2734..3528)
                     /gene="sufC"
                     /locus_tag="VFP02_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET9542904.1"
                     /translation="MSELVIRGLEAGIDGKTILRGIDLTVKGGEVHAVMGPNGSGKST
                     LSHVIMGRPGYDVTGGSVTMDGQDVLAMTPWQRAQAGLFLAMQYPTEVPGVSLEALFG
                     AAVTAAGGDIADIRERMVVEAERIGFDERFLARPLNVDLSGGEQKRNETLQLGVLLPK
                     IAILDELDSGLDIDALRTCSRRIEEATHEDGLGVLAITHYSRLFTELHPDRVHILVGG
                     RFVAEGGDELAAELEADGYARYGVESEEEPAAVPDPFADPFADPLL"
     gene            complement(3525..3833)
                     /locus_tag="VFP02_07475"
     CDS             complement(3525..3833)
                     /locus_tag="VFP02_07475"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HET9542905.1"
                     /translation="MTRLCGRDDLAPGEARRFDVDGCPIALVRIGDGFYAIGDTCSHA
                     EFSLSEGYVDADECTIECWKHGSAFSLSTGEPESLPATRPVPVYPVTVDGDDVLVTLP
                     "
     gene            complement(3830..4984)
                     /gene="sufD"
                     /locus_tag="VFP02_07480"
     CDS             complement(3830..4984)
                     /gene="sufD"
                     /locus_tag="VFP02_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET9542906.1"
                     /translation="MERFAAADRPSPAQEVWRYSRVDEIDLDSYALAPSGAGRVPDLA
                     DAPAALVVDGHLRSTADTFPAGVEVGPLAEAKAGDEALGGVLGDAPDAFAELNTAFSP
                     DPLLVRIAPGTLVDEPIVVHVHAATTGAVSFPRLVVQVGADAQVTVVEVQTSAPGVEL
                     VAAVTEIAVGDAARARHLTVQDLDPGTWQVATQVSQVGASADFLAAGAALGGAYARLR
                     TDCRLVGRGASGTLLAAYLGRDEQMMDFRTFQDHAAPDTTSELLFKGALADHARSVYT
                     GLIRVRPNARGTEAFQTNRNLKLSEHAWAESVPNLEIENNDVKCSHASAVGPVDPEQR
                     FYLESRGVPTDEAEKLIVAGFFDEVLSRFPDQRMADRVRAEIAGTLEVVA"
     gene            complement(5194..5532)
                     /locus_tag="VFP02_07485"
     CDS             complement(5194..5532)
                     /locus_tag="VFP02_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542907.1"
                     /translation="MPMRDDCKHFESRTYGDGETVRKCDLDLAPEAPWRCPDDCPSYV
                     RRSDIAWQVGTQVAPPAPPAPASLGKDDSVARLLDEAEDIINAAGPDILAELKQEKKG
                     RRGRKRRRKD"
     gene            complement(5580..6983)
                     /gene="sufB"
                     /locus_tag="VFP02_07490"
     CDS             complement(5580..6983)
                     /gene="sufB"
                     /locus_tag="VFP02_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET9542908.1"
                     /translation="MTASDTTLDLSRYKLGWSDEEDYVFKPKRGLNEDIIQEMSWMKG
                     EPDWMRKFRLKSYAQFTRRPMPDWGGDMSGIDFDNIYYYIKPTDHQVDAWDELPDSVK
                     DTYEKLGIPEAERKYLAGVTAQYESEVVYHRNREDLERQGVIFTDMDTALREHPEIVK
                     AYFGKVIPPNDNKFSSLNSAVWSGGSFIYVPPGVNVEMPLQAYFRINAENMGQFERTL
                     IIADEGSQVHYIEGCSAPVYTTDSLHSAVVEIIVKPSARVTYTTIQNWSNNVYNLVTK
                     RARVETEGHMEWIDGNIGSRLTMKYPSVYMVGPKASGEVLSVAYAGPGQHQDAGAKMV
                     HAAPETTSKIVSKSISKDGGRTSYRGLVRVEDDAYGCKSHVQCDALILDDESTSDTYP
                     YMEVGAKDAVVGHEATVSKVADEQLFYLMSRGLSEEQAMGMIVNGFIEPVTRTLPMEY
                     AVEWSRLIELQMEGSVG"
BASE COUNT         1015 a         2380 c         2584 g         1058 t
ORIGIN      
        1 gccaggactg attccggaac gacgccggcg cgccgctgtt gacgacgacc tcctggtcga
       61 gggaatcgtt gatgttggcg ccgctggtgc ccgcccaacc gttctggccg tcgatgtcgc
      121 cggccgagta ggtcacagcc gggacgggag ctgcgcccgc cggagatgcc gcgagcggcg
      181 ccaggaaccc ggcgacgatg gcgacgagcg ccacccccac gaagtggttg cgcgcgcgcg
      241 tcgtagttat tcgatcaatc aagagttgat tcctcactgt tcaggggtgg atggtcggat
      301 tcgaccggga gtggggtggg cccctggagt cacgccgtcc gccgcgccca cccgatcacc
      361 cgcaggtgcg ggccggcaga agataccagc ccgatgcgac aaatcggtgc atggtgtctc
      421 acccggacgg gtgaccgtga ccggcgcgtc agagggcgta caaggcggag gcgcgggcct
      481 cggtcaggga gaggacccag ccgtcgagca cgatcacctc gcccgtcgcg tggtcctccg
      541 aggcctgcgg ggcgaggacc cggaggtcct ggcccggccg ctcgggcacc gtgccgtcca
      601 gggccgggag cgccgccagc agcacctgct cgtcggcctc gtcgggggcg acctcgaggt
      661 accggccccc cacggccgcg agggcctcgg ccagcgacga ggtgggttcg ggcggcggct
      721 cggtcgtcgc cggagccgcg ggagcatcgt cgtcgtccag cagccgccag ccgacgaccc
      781 cgccgccgac gacgaccgcc gcggctccga ggcccttgag caccgaccgt cggtccgggc
      841 tcacaccagg acctcccgct gcaggtggtc ggccagccgc agcgtcagcg ccaccagcgt
      901 gtaggtcggg gtggaggcgc cggcggtgcc gaaggaggag gagccggtca cgtagaggtt
      961 gtcgaccgtg tggacccgca ggtcggcgtc gaccacgccc tcggccgggt cggcggccat
     1021 ccgggtggtg cacatgtggt ggaagcccag gcccagctcg aagccctcgg gatcggcggc
     1081 gtcggcgtcg atggccaggc ccccgacgta gggacggatg ccgtcggtgc gctcgaaggt
     1141 gatcgtgccg ggggtcagct gcaaccgtcc gaggcccgtg cgggccagct catcgccgat
     1201 gacggccatg ccctcgagga tcgacgagcg gtcccgctcg gtgaactgcc agtccaactc
     1261 gacccggggc atccccagcg ggtcccgctc agaaccgagc cggacacggg agtcggggtt
     1321 caactcctgc tcggcggtgg ccaggaagta gagcgacgag cggggggcgt cggtgccggt
     1381 ggcctgggcc acggccgcca cctggctgat cccgaggcca ctgatggccc gaggccgctc
     1441 ctcggcgtag gccccgacca cgagctggca accgagcgac aacagcccgc ggtcacggac
     1501 cgccgccgag gtcagggcca cgtcgccctt gatgcgcaac gtgccctcct cggcgccctg
     1561 gtcggcctcg aacagggcgc tgagcgcagc cgggtcgttg ccggtgacgg cgatggccgc
     1621 cggcaccgcg aagtgctcgc agaagtggcg gcccaccagg tcggcttcgt tgcccaggcc
     1681 ggcggggcgc tggctgttgc tggccagcag gaggcggggg ttctcgatgc cgccgagggc
     1741 gacgacgacg acctgaggct cgacgacacc gaactcgacg ccgccgaggg tcttggcccg
     1801 gacctccacg acccggtcgc cggcgtcgtc gaggcggaga tcgacggcgt tggcccgcag
     1861 gtacacggtg acgtcggtgg cggcgtccag ctcgtcccgg tacatcggcc cgaagcgagc
     1921 gtcccggatc tggtacacgc ccgtctgcac gagcggggtg tcgatcaggg gctcaccccc
     1981 acccagctcg tcgacccagt actcccagtc gaagcggtag gggccaaggc cgaggacggg
     2041 atgggcggcc tggtaccacg ggtcgatgtc cgcccgcgtg atgggccagc ccgagcgctc
     2101 cagagcgggc cggacctcga agtcgacctc gtcgtagggg cggcagaacc cggcccagtg
     2161 gttggtcgtc ccgccgagct gacgcaggcg gatgctgtcc agggtggccg ggttgtccga
     2221 gaagccgagg gtctcgccca ccgagtcgcc ccgggccagg tcctggatgt cgggctcgag
     2281 cgcctccgcc ccgctctcca ggaccacgac ctgcaggccc gtgccctcca agtcccgggc
     2341 cagggtgatc cccgccgcgc cggcgccgag aatgcagacg tcggccgtca ggcgggaccc
     2401 cgaatcgagg tcctcggcat cgaggatcac cctgggctac tcgccgaggg cgcggtcgac
     2461 ccgagtggcc agcgagacat cgagctcggt caggccgccg gcgtcgtggg tcatcaggtc
     2521 gatggtgacc cggttgtaga cgttggacca ctcggggtgg tggttgaggt gctcggccca
     2581 cacggccacg tgcgccatca ggccgaacgc ctcggcgaag tcgcccagga cgatgtcccg
     2641 gtggagcttg ccgtcgacca ccgaccagtt cggcagcgtg gtcagagcct cagccagttc
     2701 atcgtcggtc agtcggtcag gcatggtgtc acctcagagg agcgggtcgg cgaagggatc
     2761 ggcgaacgga tcgggcacgg cggccggctc ctcctcggac tcgaccccgt agcgggcgta
     2821 gccgtcggcc tccagctcgg cggccagctc gtcgcccccc tcggccacga accggccgcc
     2881 gaccaggatg tggacgcggt cggggtggag ctcggtgaag aggcggctgt agtgggtgat
     2941 cgccagcacg cccaagccgt cctcgtgggt ggcctcctcg atgcggcggc tgcacgtccg
     3001 aagagcgtcg atgtcgaggc cggagtccaa ctcgtcgagt atggcgatct tgggcaggag
     3061 cacgccgagc tggagcgtct cgttgcgctt ctgctcgccg ccggacaggt cgacgttgag
     3121 cggccgggcc aggaagcgct cgtcgaagcc gatgcgctcg gcctcgacca ccatgcgctc
     3181 ccggatgtcg gcgatgtcac caccggcggc ggtcacggcc gcgccgaaga gggcctcgag
     3241 agacaccccg gggacctcgg tcgggtactg catggccagg aagagcccgg cctgggcccg
     3301 ctgccacggc gtcatggcca gcacgtcctg gccgtccatc gtcaccgagc cacccgtgac
     3361 gtcgtagccg ggacggccca tgatcacgtg ggagagcgtc gacttgcccg agccgttggg
     3421 gcccatgacg gcgtgcacct cgccgccctt cacggtgagg tcgatgcccc gcaggatggt
     3481 cttgccgtcg atgcccgctt cgaggccccg gatcaccagc tcgctcatgg cagcgtcacc
     3541 aacacgtcgt cgccgtcgac ggtcacgggg tacaccggga ccggtcgggt ggcggggagc
     3601 gactccggct cgccggtgga cagcgagaag gcgctgccgt gcttccagca ctcgatggtg
     3661 cactcgtcgg cgtccacata gccctcggac agcgagaact cggcgtggga gcaggtgtcg
     3721 ccgatggcgt agaacccgtc gccgatgcgg accagagcga tcgggcaccc gtccacgtcg
     3781 aagcgccggg cctcgcccgg ggcgaggtcg tcacgcccac agaggcgggt cacgccacca
     3841 cctccagggt gccggcgatc tcggcccgca cccggtcggc cattcgctgg tcggggaagc
     3901 ggctgaggac ctcgtcgaag aacccggcca cgatcagctt ctcggcctcg tcggtgggca
     3961 cgccacggct ctcgaggtag aagcgctgct cgggatcgac cggaccgacg gcggaggcgt
     4021 gcgagcactt cacgtcgttg ttctcgatct cgaggttggg taccgactcg gcccaggcgt
     4081 gctcggacag cttcaggttc cggttcgtct ggaaggcctc ggtcccccgg gcgttggggc
     4141 ggacccggat gaggccggtg tagacggagc gggcgtggtc ggccagcgct cccttgaaga
     4201 gcagctcgct cgtggtgtcg ggcgcggcgt ggtcctggaa ggtccggaag tccatcatct
     4261 gctcgtcgcg cccgaggtag gcggccagga gcgttcccga tgcgccccga ccgaccaggc
     4321 ggcagtcggt gcgcagccgg gcgtaggccc cgcccagcgc ggccccggcg gcgaggaagt
     4381 cggcgctggc cccgacttgg ctgacctgcg tggccacctg ccacgtgcca gggtcgaggt
     4441 cctgcacggt gagatggcgg gcccgggcgg cgtcgcccac ggcgatctcg gtgacggcgg
     4501 caacgagctc gacgccgggg gctgaggtct ggacctcgac cacggtgacc tgggcgtcgg
     4561 cgccgacctg gaccaccaga cgggggaagc tgaccgcacc ggtggtggcg gcgtggacgt
     4621 ggaccacgat cggctcgtcg accagggtgc ccggcgcgat ccggacgagg agcggatcgg
     4681 gcgagaaggc cgtgttgagc tcggcgaagg cgtcgggggc gtcgccgaga acgccgccga
     4741 gggcctcgtc gccggccttg gcctcggcca gtgggccgac ctcgacgccg gcgggaaacg
     4801 tgtccgcggt ggagcggagg tggccgtcca ccaccagggc agcaggagcg tcggcgaggt
     4861 cgggcacccg accggccccg ctcggggcga gggcgtagct gtcgagatcg atctcgtcga
     4921 cccggctgta gcgccatacc tcttgggccg ggctcgggcg atccgccgcc gcgaagcgct
     4981 ccacggcctc gacccgacgg gcccgcagcc agtcggggcc agcgagggcg gcggcagcgt
     5041 cgggggtcag ggcggtcagg atcggctccg gcggagggtg agggctgctc agtctacggc
     5101 aggggccaga acgcccaacg gcgggccgcc ctccgaaacg gcgtcaagtg gtgacgctcg
     5161 acgtgaccca cctgctgcag aaaggcccgg gcttcagtcc ttgcgccgac gcttgcggcc
     5221 ccgacggccc ttcttctcct gcttcagctc ggccaggatg tccggcccgg cggcgttgat
     5281 gatgtcctcg gcctcgtcga gcaggcgggc gacgctgtcg tccttgccga ggctggccgg
     5341 ggccggcggg gccggcgggg cgacctgcgt gccgacctgc caggcgatgt cggagcgacg
     5401 cacatagctc gggcagtcgt ccgggcagcg ccacggggcc tccggggcga ggtcgaggtc
     5461 gcacttgcgc acggtctcgc cgtcgccgta tgtccgactc tcgaagtgct tgcagtcgtc
     5521 ccgcatcggc atacgcccat cgtacgcccc acccccaccg acccgatggc ggccctgggt
     5581 cagccgacgg agccttccat ctggagctcg atgaggcggg accactcgac ggcgtactcc
     5641 atgggcagcg tgcgggtgac gggctcgatg aacccgttga cgatcatgcc catggcctgc
     5701 tcctcggaga ggccacggct catcaggtag aagagctgct cgtcggccac cttggagacg
     5761 gtggcctcgt ggcccacgac ggcgtccttg gccccgacct ccatgtaggg gtaggtgtcc
     5821 gaggtgctct cgtcgtcgag gatgagggcg tcgcactgga cgtggctctt gcagccgtag
     5881 gcgtcgtcct ccacccgcac gaggccccgg tacgaggtcc ggcccccgtc cttggagatg
     5941 gacttcgaca cgatcttgga cgtggtctcc ggagcggcgt ggaccatctt ggcgccggcg
     6001 tcctggtgct gacccgggcc ggcgtaggcg accgagagga cctcgcccga cgccttgggc
     6061 ccgaccatgt agacggacgg gtacttcatc gtcaggcggg agccgatgtt gccgtcgatc
     6121 cactccatgt ggccctcggt ctcgacccgg gcccgcttgg tgaccaggtt gtagacgttg
     6181 ttggaccagt tctggatggt cgtgtaggtg acccgggcgg acggcttcac gatgatctcg
     6241 accacggccg agtgcagcga gtcggtggtg tacacggggg cggagcagcc ctcgatgtag
     6301 tgcacctggc tgccctcgtc ggcgatgatc agggtccgct cgaactgacc catgttctcg
     6361 gcgttgatgc ggaagtaggc ctgcagcggc atctcgacgt tgacaccggg cggcacgtag
     6421 atgaacgacc cgcccgacca gacggccgag ttcagcgacg agaacttgtt gtcgttgggc
     6481 gggatcacct tgccgaagta cgccttgacg atctcggggt gctcccgcag cgcggtgtcc
     6541 atgtccgtga agatcacgcc ctggcgctcg aggtcctcgc ggttgcggtg gtagacgacc
     6601 tcggactcgt actgcgcggt gaccccggcc aggtacttgc gctcggcctc ggggatgccc
     6661 agcttctcgt aggtgtcctt gaccgagtcg ggcagttcgt cccaggcgtc gacctgatgg
     6721 tcggtcggct tgatgtagta gtagatgttg tcgaagtcga tgccgctcat gtccccgccc
     6781 cagtcgggca tgggccggcg ggtgaactgg gcgtaggact tcagccggaa cttgcgcatc
     6841 cagtccggct cgcccttcat ccaggacatc tcctggatga tgtcctcgtt caggccgcgc
     6901 ttcggcttga agacgtagtc ctcttcgtcg ctccagccca gcttgtaccg agacaggtcg
     6961 agcgtcgtgt cgctggcggt catgcagagc ctcctacccc gggctccggg gcaatttctc
     7021 ctacgtagat agtaaca
//
LOCUS       DASTFV010000405         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1499228, whole genome shotgun sequence.
ACCESSION   DASTFV010000405 DASTFV010000000
VERSION     DASTFV010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499228"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1737)
                     /locus_tag="VFP02_07495"
     CDS             complement(184..1737)
                     /locus_tag="VFP02_07495"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET9542909.1"
                     /translation="MSDSKRVVIFDTTLRDGEQSPGISLDVGEKLEIAEQLARLGVDY
                     LEAGFPVASQGDFEAVEAIARSVRGPVITGLSRTALKDVDRCWEAVQHAEQARIHVFI
                     ATSENHMANKLKMTPDQVKVEAGSAVAHAKNYTDDVEFSPEDASRSDFDFMCEVLQIA
                     VDSGAVTLNIPDTVGFAIPQEFGRRIAAIRERVTGDYVISAHCHDDLGMAVANSLAAV
                     EAGARQVEVAMNGIGERAGNAALEEIVMALRTRSEYFGGLDTNVRAEELAKTSRLVAR
                     LTGYPVQYNKAVVGRNAFAHEAGIHQHGMLEDRTTYEIMDPNAVGQDTSLVLGKHSGR
                     HAFSDTLEKMGLKVHGDALNQAFARFKELADRKVEITDADLEAIVAAELNVSSTDAFE
                     LSSIEFRGSNVGPASAQVVLVRTTDGEKMEATAEGDGMIDAAGQAIRSATGVDGRQTD
                     FNVSSVTGGVDALGDVVIQFESDGVKVSGRGVSTDVVEASARALLNAINKVIRIRDRA
                     EVRDTVTGP"
     gene            2003..>2416
                     /locus_tag="VFP02_07500"
     CDS             2003..>2416
                     /locus_tag="VFP02_07500"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9542910.1"
                     /translation="MGDASATRSTTVNAPVEAVWATLAAFDRLSTWSPEVDHSTYLTE
                     QREGVGSARRVQVGRTVLVERITEWDPPRALAYALEGLPPVAGGATNRWTLRAEGDRT
                     FVTLTSTVDPGAKPVGRVAAPLVARRLAKASDGMLA"
BASE COUNT          369 a          840 c          815 g          392 t
ORIGIN      
        1 ccttgcggac cgatccactg ggcgggcacc gagacggcca cccgttggat gggctacatc
       61 gacggcgccg tccagtccgg ggagcgagcc gccagcgagg tcctcaccgc cctgacgtag
      121 ccgggaacca catcgggacc tcgggtcgtc ggaggactcc gccgaaagcc cgaaagctct
      181 cggtcacggg ccggtaacgg tgtcccgcac ctcggcccgg tcccggatcc ggatcacctt
      241 gttgatcgca ttgagaaggg cccgcgccga cgcctccacc acatcggtgg agaccccacg
      301 ccccgacacc ttcacgccat cggactcgaa ctggatgacc acgtcgccta gggcgtcgac
      361 gccgccggtc accgacgaca cgttgaagtc ggtctggcga ccgtcgaccc cggtggcgct
      421 gcggatggcc tggccggcgg cgtcgatcat gccgtcaccc tcggccgtgg cttccatctt
      481 ctcgccgtcc gtggtgcgga ccagcacgac ctgcgcgctg gccgggccga cgttgctgcc
      541 gcggaactcg atgctggaca gctcgaaggc gtcggtggat gacacgttca gctcggcagc
      601 cacgatggcc tcgaggtcgg cgtcggtgat ctccaccttc cggtcggcca gctccttgaa
      661 ccgggcgaaa gcctggttca gagcgtcgcc gtggaccttg aggcccatct tctccagcgt
      721 gtcgctgaag gcgtggcgac cggagtgctt gcccaacacc agcgaggtgt cctggcccac
      781 ggcgttgggg tccatgatct cgtaggtggt gcggtcctcc agcatcccgt gttggtggat
      841 gcccgcctcg tgggcgaacg cgttccggcc gacgacggcc ttgttgtact gcaccgggta
      901 gccggtcagc cgggcgacca gccgactggt cttggccagc tcttcggccc ggacgttggt
      961 gtccaggcca ccgaagtact ccgaccgggt gcgcagggcc atgacgatct cctcgagggc
     1021 ggcgttgccg gcccgctccc cgatgccgtt catggccacc tcgacctgtc gggccccggc
     1081 ctccaccgcg gccagggagt tggccacggc catgcccagg tcgtcgtggc agtgggccga
     1141 gatgacgtag tcaccggtga cccgctcccg gatggcggcg atgcgccggc cgaactcctg
     1201 ggggatggcg aagccgacgg tgtcggggat gttcagcgtg accgcaccgc tgtcgacggc
     1261 gatctgcagg acctcgcaca tgaagtcgaa gtccgagcgg gaggcgtcct cgggcgagaa
     1321 ctcgacgtcg tcggtgtagt tcttggcgtg agccaccgcc gaacccgcct ccaccttcac
     1381 ctggtccggg gtcatcttca gcttgttggc catgtggttc tcggacgtgg cgatgaagac
     1441 gtggatgcgg gcctgctcgg cgtgctggac ggcctcccag caccggtcga cgtccttcag
     1501 ggccgtgcga gacaggccgg tgatgaccgg gccccgtacc gaccgggcga tggcctcgac
     1561 ggcctcgaag tcgccctgac tggcgaccgg gaagccggcc tccaggtaat cgacccccag
     1621 acgggcgagc tgctcggcga tctccagctt ctcgccgacg tccagcgaga tgcccggcga
     1681 ctgctcgccg tcccgcagcg tcgtgtcgaa gatcacgact cgcttgctgt cactcatggg
     1741 actgcctttc tggggttcgg gggtgatggt cacggcattg cggtccaggt ccgaaaacga
     1801 aaaaaccccc tgacccttcc gggttcagga ggcgagacga gcaccgatat gggggtgctc
     1861 gccttacgta agaaggaggt ccagggcgcg acggtcgaaa cgcatctcgg gcaccatcgc
     1921 accggatcgc cgaatcgtca acccccgaca atcatttgac actcgaagtg ccactattgt
     1981 ggccaactgc tagacagaac ccgtggggga tgcctcggcc acacgctcga ccacggtcaa
     2041 cgcacccgtg gaggccgtct gggcgaccct ggccgcgttc gaccgcctca gcacctggtc
     2101 gcccgaagtg gaccactcga cgtatctgac cgagcagcgc gagggtgtcg gcagcgcccg
     2161 gcgggtgcag gtgggacgaa ccgtgctggt cgagcgcatc accgagtggg acccgccccg
     2221 agccctcgcc tatgccctcg agggcctgcc ccctgtcgcc ggcggggcca ccaaccgctg
     2281 gaccttgcgg gcggagggcg accggacgtt cgtcacgctg accagcaccg tcgatcccgg
     2341 ggcaaagccc gtcgggcggg tcgccgctcc gctcgtcgcc cgtcgcctgg ccaaggcgtc
     2401 cgacggcatg ctcgcc
//
LOCUS       DASTFV010000406         3047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1504074, whole genome shotgun sequence.
ACCESSION   DASTFV010000406 DASTFV010000000
VERSION     DASTFV010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504074"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VFP02_07505"
     CDS             <1..793
                     /locus_tag="VFP02_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542911.1"
                     /translation="PPSVITENQADCLSGAWVAQQVDDGYGPFADPGALDQAIGAMIY
                     VRDTPGSDPGEALAHGSGFDRVRAFQDGVDQGIEYCFNYINDPPFIDELPFTDENDFA
                     NHGNLPFDEVTELVIQDLDDFFGENVDGFESPGDPFDVIPQDELEQLHAEIGDGAVAT
                     LFGMLWAERAQAAAGDDLDGEGPLLQRSCLVGAWLGDILRDQQDGQVDRESGVQLSPG
                     DLDETIITFLQLTAQAMEEGGVAFEAIGDMRTGVFGGVDDCRLGE"
     gene            883..1566
                     /locus_tag="VFP02_07510"
     CDS             883..1566
                     /locus_tag="VFP02_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9542912.1"
                     /translation="MAGERLLLVDDEENLRSMLDAALRHSGFDVVPVGSGREALDAVK
                     AERPELIVLDVMLPDIDGFEVCRRLRTEGDRTPVLFLTARDATEDTVRGLTLGGDDYL
                     VKPFSLEELVARIGSVLRRTGTAKPGSRLACADLELDDDAHRVTRAGEPVNLSPTEYN
                     LLRYLLVNQGQALSKAQILDHVWQYDFGGDGGVVETYIGYLRRKVDHVAPKLIHTIRG
                     VGYSLRESP"
     gene            1576..2985
                     /locus_tag="VFP02_07515"
     CDS             1576..2985
                     /locus_tag="VFP02_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9542913.1"
                     /translation="MSLRARVLLGLGVIALVFGVTAVVVPRITASYLLEQVDDQLRAT
                     DFPYRGDLAVGAERGRPSSTPDDQTPPERPSRMYIGIVDADADADVSEIFAPNVGDTA
                     APSIDGEQAIAEAGNGPYTQPAGDSGSRFRLVSREVDDGDTDGDVVVVGLPLDDVDAT
                     VSRLVQIEVGALALVLFVLAMVAWWVIRLGVRPVKAMAAAATDIGAGDLSARVPEYGE
                     ATEAGQLGTALNQMLERIEDEFDQRLRSEQRLRQFAADASHELRTPVTTVRGYAELYR
                     TGALDDPAELTEAMRRVEQESVRMGSLVDDLLRLARLDQGRELERVPVDLGAVVTDAG
                     RDAQATDPERPVTVEVEDPVVVIGDERLLRQVVTNLTGNALVHTDPEVAVTLRAAVEG
                     DRAVLEVRDAGPGMRPDVAERVFERFYRDDPSRSRHRGGSGLGLAIVQATVIAHGGEI
                     SVDTAPGEGTTFLVTLPLT"
BASE COUNT          452 a         1092 c         1061 g          442 t
ORIGIN      
        1 cccgccgagc gtgatcaccg agaaccaggc cgactgcctt tcgggggcct gggtggccca
       61 gcaggtggac gacggctacg gccccttcgc cgatcccggg gccctggacc aggccatcgg
      121 ggccatgatc tacgtccggg acacgccggg aagtgacccg ggagaggcac tggcccacgg
      181 cagcggcttc gatcgggtgc gggccttcca ggacggggtc gaccagggca tcgagtactg
      241 cttcaactac atcaacgacc cgccgttcat cgacgagctg cccttcaccg acgagaacga
      301 cttcgccaac cacgggaacc tgcccttcga cgaagtgacc gagctggtga tccaggacct
      361 cgacgacttc ttcggcgaga acgtcgacgg cttcgagagc cccggtgacc ccttcgacgt
      421 gatcccccag gacgagctcg agcagctcca cgccgagatc ggcgacggcg cggtggccac
      481 cctcttcggc atgctctggg ccgaacgggc ccaagccgcg gcgggcgacg acctcgacgg
      541 cgaggggccc ctcctgcagc ggtcctgcct ggtgggggcg tggctgggcg acatcctccg
      601 cgaccagcag gacggccagg tcgaccggga aagcggcgtg cagctgtcac cgggcgacct
      661 cgacgagacc atcatcacgt tcctgcagct gaccgcgcag gccatggagg agggcggggt
      721 ggccttcgag gccatcggtg acatgcgcac cggggtcttc ggcggcgtcg acgactgtcg
      781 gttgggggag tagtcgtcgg ctctcaggca actcccaggt tctgtcctgt cggctcccag
      841 caggacggtc cagactgagg gtcgtgcctg acaggacccg ccatggccgg tgaacgactt
      901 ctgctcgtcg acgacgagga gaacctccga tccatgctcg acgctgcgct ccgccacagc
      961 gggttcgacg tggtcccggt gggcagcggc cgggaggcgc tggacgcggt gaaggccgag
     1021 cggcccgagc tcatcgtgct cgacgtgatg ctccctgaca tcgacggctt cgaggtgtgt
     1081 cgccgcctgc gcacggaggg cgatcgcacg ccggtgctgt tcctgaccgc ccgcgacgca
     1141 accgaagaca ccgtccgggg cctgaccctg ggcggtgacg actacctggt gaagcccttc
     1201 agcctggagg agctggtggc tcgcatcggc tcggtcctcc ggcggacggg cacggccaag
     1261 cccggctccc gcctggcctg cgccgacctg gagctggacg acgacgctca ccgggtgacc
     1321 cgggcgggcg agccggtcaa cctgtcaccg accgagtaca acctcctgcg ctacctgctg
     1381 gtcaatcagg gccaggccct gtcgaaggcc cagatcctgg accacgtgtg gcagtacgac
     1441 ttcggcggcg acggcggcgt ggtggagacc tacatcggct acctgcgccg caaggtcgac
     1501 cacgtcgccc ccaagctgat ccacaccatc cggggcgtgg gctactccct ccgcgagtcc
     1561 ccctaggcgg cccgggtgtc cttgcgggcc cgggtcctgc tcgggctggg ggtgatcgcc
     1621 ctggtcttcg gggtgaccgc ggtggtcgtg ccccgcatca ccgcgtccta cctgctcgag
     1681 caggtcgacg atcagctccg ggccaccgac ttcccgtacc ggggggatct ggccgtcggc
     1741 gccgaacgcg gtcgcccatc ctcgaccccg gacgaccaga ccccgcccga gcgcccgagc
     1801 cggatgtaca tcggcatcgt ggacgccgac gccgacgccg acgtgtcgga gatcttcgcc
     1861 cccaacgtcg gggacaccgc ggccccgtcc atcgacggcg agcaggcgat cgctgaggcg
     1921 ggcaacgggc cgtacacgca gcccgccggc gactcgggca gccgtttccg actcgtcagc
     1981 cgtgaggtgg atgacggtga caccgacggc gatgtcgtgg tcgtcggcct gccattggac
     2041 gatgtcgacg ccacggtgtc gcgcctcgtc cagatcgaag tcggtgccct tgccctcgtg
     2101 ctcttcgtgc tcgcgatggt ggcctggtgg gtgatccgtc tgggtgtccg accggtcaaa
     2161 gcgatggccg cggcggccac cgacatcggc gccggcgacc tgtcggcccg ggtcccggaa
     2221 tacggcgagg ccaccgaggc aggtcagctc ggtaccgcgc tcaaccagat gctggagcgg
     2281 atcgaggacg agttcgacca gcgcctccgc tccgagcagc gcctgcgcca gttcgcggcc
     2341 gacgcttcgc atgagctgcg gaccccggtg accacggttc ggggctacgc cgagctctac
     2401 cgcaccggcg ccctggacga cccggccgag ctgaccgagg ccatgcgccg ggtcgagcag
     2461 gagtcggtgc gaatgggctc gctggtcgac gacctgctgc ggttggctcg cctcgaccag
     2521 ggccgcgagc tggagcgtgt ccccgtcgac ctcggggcgg tggtgaccga cgccggccgt
     2581 gacgcccagg ccaccgatcc ggaacggccg gtcaccgtcg aggtggagga tccggtcgtc
     2641 gtcatcggtg acgaacggct gctgcgccag gtcgtcacca acctgaccgg caacgccctg
     2701 gtccacaccg atccggaggt ggccgtgacc ctgcgggccg ccgtcgaggg cgaccgagcc
     2761 gtgctcgagg tgcgcgacgc cggcccgggc atgcgccctg acgtggccga gcgggtcttc
     2821 gaacgcttct accgagacga cccctcccgg tcccggcacc ggggcggcag cggcctcggc
     2881 ctggccatcg tccaggccac cgtcatcgcc cacggcggcg agatcagcgt cgacacggcc
     2941 cccggcgaag gcaccacctt cctcgtcacc cttcccctga cctgacccgg tcaacggtga
     3001 cgcaacgccg tcatcagccg acggtgccgt ccccgttcgg gcgggac
//
LOCUS       DASTFV010000407         5344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1506291, whole genome shotgun sequence.
ACCESSION   DASTFV010000407 DASTFV010000000
VERSION     DASTFV010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506291"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VFP02_07520"
     CDS             complement(<1..726)
                     /locus_tag="VFP02_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HET9542914.1"
                     /translation="MKRFTPQQLVIGLGVAIALFTIGSGLAPGLTGFEEESSIHRVVF
                     GNIPDALKVTFYTVIPALLVYGAVLFSFRVRNWQRGAPDDRSTNAGNIKRRLGDFRAG
                     VYMQTLLRDPVAGVMHTLIYVGFLVLTAVTAVLEIDHQLPDDAKFLHGTTYQAYAFVG
                     DAAGLVFLAGIAIAVVRRYVIRPYRIRIKSKPEHALILGLFAVLGLTGFAAEMLRIAL
                     NGRPAYEEWSFVGYPLSSLVDGMD"
     gene            921..2360
                     /locus_tag="VFP02_07525"
     CDS             921..2360
                     /locus_tag="VFP02_07525"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET9542915.1"
                     /translation="MGEYRQFIGGQWTDASDGGTWDLIDPATEATIRTVPFGTGADAT
                     AAIDAAAAAFPAWSTVTAYERAAVLRRASDLVRERLDALGEVTSRESGKPVVQAKGEW
                     VASADLLEWYGEEAKRSYGRIIPSRVPGKRMLVLHQPVGVVGTITAWNFPAYNVARSV
                     AAALAAGCTVVSRPSEFTPMTCMDLAGILQEAGLPDGVFNVVNGDPEQMGQAMLEHEA
                     LRKLHFTGSVRVGKLLMDGASKTVTRLSLELGGNAPVLILPDADLEVVAAGAVAAKYR
                     NAGQVCVSPQRFLVGGERRDEFVERVVPLVEKLRLGAGTDPDTDVGPMINARQRDRLE
                     GIVSDAAERGATVATGGGRPGDRDNGYFFSPTVVTDITPEFPVYHEELFGPVMPVVDF
                     GDLDEAIALANDTAYGLAAYVWTNDLRSAMRAAERLEFGMVGVNEWAPNGTEGPFTGW
                     KQSGLGSESGAEGFEEYVETKLISLGGMG"
     gene            2365..3000
                     /locus_tag="VFP02_07530"
     CDS             2365..3000
                     /locus_tag="VFP02_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET9542916.1"
                     /translation="MDDGTRSLVVRVWLPDRPGALGAVASRIGGVGGDVHEIEVVDRG
                     AGRAVDEFLVTVPEEVSDDLVAREIEAVDGVDVEELRSVEFVERDPRIDALQAAVALN
                     QADPGLELAAELASRAKLELRAVWAAVVGPEGVEAVVGVAPEAAWLSAFGAGVAAGGR
                     GDAGVAADEDGVVAWAAAGEGRVLLTGRSGVPFRPRERRVLGLLGDLAATR"
     gene            3072..3632
                     /locus_tag="VFP02_07535"
     CDS             3072..3632
                     /locus_tag="VFP02_07535"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9542917.1"
                     /translation="MMAVSGTHVPPTEAADPSPVHRAFGFSDICGFTAYLEREGPLAA
                     VRLVSAFRSSAREIAARRGVRVAKWLGDGVMFVSVEAGPQLATSVELTNRMATTGLHL
                     RSGVATGSCLLMEADDYIGHSVNLAARLCDLAGPGEVLADENTATLAPDWVEVGPPRR
                     KRLPGVGKVEGICSLRLDADLPLLTA"
     gene            3683..3943
                     /locus_tag="VFP02_07540"
     CDS             3683..3943
                     /locus_tag="VFP02_07540"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HET9542918.1"
                     /translation="MVVERYLRGPGPVYSRVEAKGRMLPDGLRYVDSWVVDDVPMDRC
                     FQLMETDDPSILDDWMALWSDLTEFEVYPVLSSSEAALRALD"
     gene            complement(3954..4727)
                     /locus_tag="VFP02_07545"
     CDS             complement(3954..4727)
                     /locus_tag="VFP02_07545"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HET9542919.1"
                     /translation="MSPSLSTSRLILLRHGQSTWNEANLFTGWYDADLTELGRAEALR
                     GGEQLAEAGILPDVVHTSLQRRAIHTGQLALDALGRDWLPVRRHWRLNERHYGDLQGK
                     DKKQVAEEHGAEKVKIWRRSYDTPPPPLDRDDERHARFDPRYADLAPELIPASECLKD
                     VVVRMLPYWYDAVVPDLRAGRVVLLAAHGNSLRALVKHLDGIADAAITEVNIPTGIPL
                     VYELDADLHPVGDVPLEDRYLGGAAAARAAADAVAKQAG"
     gene            4798..>5344
                     /locus_tag="VFP02_07550"
     CDS             4798..>5344
                     /locus_tag="VFP02_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET9542920.1"
                     /translation="MPKAVGIDLGTTNSVVAVLEAGEATVIPNAEGSRTTPSVVAFAK
                     DGEVLVGEVAKRQAITNPDRTVRSVKRHMGTSWSIDIDGKSYKSQEIAARVLQKLKAD
                     AEAYTGETVNQAVITVPAYFDDAQRTATQEAGQIAGLEVLRIINEPTAAALAYGMDKE
                     GEDQTILVFDLGGGTFDVSLLD"
BASE COUNT          803 a         1843 c         1941 g          757 t
ORIGIN      
        1 gtccatgccg tcgaccaggg acgacagcgg gtagcccacg aaggaccact cctcgtaggc
       61 gggccggccg ttgagggcga tgcggagcat ctcggcggcg aagccggtca ggccgagaac
      121 ggcgaacagg ccgaggatca gggcgtgctc gggcttggac ttgatgcgga tccggtaggg
      181 gcggatcacg tagcggcgga cgacggcaat ggcgatgccg gccaggaaga ccagcccggc
      241 ggcgtcgccc acgaaggcgt aggcctgata ggtcgtgccg tggaggaact tggcgtcgtc
      301 ggggagctgg tggtcgatct ccagcacggc ggtgaccgcg gtcagcacga ggaacccgac
      361 gtagatcaac gtgtgcatga cgccggcgac gggatcccgc agcagggtct gcatgtagac
      421 gccggcccgg aagtcgccca gacggcgttt gatgttgcca gcgttcgtcg accggtcgtc
      481 gggggcaccc cgctgccagt tgcggacccg gaacgagaac agcacggcgc cgtagaccaa
      541 cagggccggg atgaccgtgt agaaggtgac cttcagggcg tcggggatgt tcccgaacac
      601 cacgcggtgg atcgaggact cctcctcgaa gccggtcagg ccgggggcca ggcccgagcc
      661 gatcgtgaag agggcaatgg cgacaccgag gccgatgacc agttgctgag gggtgaaccg
      721 cttcatgacc ccgctgaacc tactccgcgg gggccggtcg gcctcgaacc cacaatcggg
      781 cccgacaagc cgtcacggac cgtcaacatc ggcgaaacac gcccgcctta gggtcggcgg
      841 ggtgctgctg acggcgcccg tcgcccaccc gtacgctgcc cgacgaacag gtcgacgacg
      901 acgtctccac ggggggatcc atgggtgagt accggcagtt catcggcggg cagtggaccg
      961 acgcatccga cggtgggacg tgggacctga tcgatcccgc caccgaggcc acgatccgca
     1021 ccgtgccctt cgggaccggg gccgacgcca ccgccgccat cgacgccgcg gcagccgcgt
     1081 tcccggcctg gtccacggtg acggcctacg agcgcgcggc cgtcctgcgc cgggcttcgg
     1141 acctggtgcg ggagcgtctc gacgccctcg gcgaggtcac gtcgcgcgag agcgggaagc
     1201 cggtggtcca ggccaagggc gaatgggtgg cgtcggccga cctgctcgag tggtacggcg
     1261 aggaggccaa gcgcagctac ggccgcatca tccccagccg ggtcccgggc aagcggatgc
     1321 tggtcctcca ccagcccgtc ggcgtcgtgg gcacgatcac ggcctggaac ttccccgcct
     1381 acaacgtggc ccgctcggtg gcggcggcgc tggccgcggg ctgcaccgtg gtcagccggc
     1441 cgtcggagtt cacgcccatg acctgcatgg acctggccgg catcctccag gaggccggcc
     1501 tgcccgacgg ggtcttcaac gtggtcaacg gcgatcccga gcagatgggc caggccatgc
     1561 tcgagcacga ggccctgcgc aagctgcact tcaccggcag cgtccgggtc ggcaagctcc
     1621 tgatggacgg cgcctccaag accgtcaccc ggctgtccct ggagctgggg ggcaacgccc
     1681 ccgtgctgat cctgcccgac gccgacctcg aggtcgtggc ggccggggcg gtggcggcca
     1741 agtaccgcaa cgccggccag gtctgcgtct ccccgcagcg cttcctcgtc gggggggagc
     1801 gtcgggacga gttcgtggag cgggtcgtcc cgctggtcga gaagctccgc ctcggggcgg
     1861 gcacggaccc ggacaccgat gtcgggccga tgatcaacgc ccggcagcgg gaccggctgg
     1921 agggcatcgt cagcgacgcc gccgagcggg gggcgaccgt cgccacgggc ggcggtcgac
     1981 cgggtgatcg ggacaacggc tacttcttct cgcccaccgt cgtcaccgac atcacccccg
     2041 agttccccgt ctaccacgag gagctgttcg gcccggtcat gccggtcgtc gacttcggcg
     2101 acctggacga ggccattgcg ctggccaacg acaccgccta cggcctggct gcctacgtct
     2161 ggaccaacga cctgcggtcg gccatgcgag cggccgagcg tctcgagttc ggcatggtcg
     2221 gggtcaacga atgggcgccc aacggcaccg aaggcccctt caccggctgg aagcagagcg
     2281 gcctcggcag tgagtccggg gcagagggct tcgaggagta cgtcgagacc aagctcatct
     2341 ccctgggcgg catggggtag cgccatggac gacggcaccc gctcgctcgt ggtccgggtc
     2401 tggctgcccg accgcccggg ggcgctggga gcggtggcca gccggatcgg cggcgtcggc
     2461 ggagatgtgc acgagatcga ggtcgtcgac cggggcgcag gccgggccgt ggacgagttc
     2521 ctggtgaccg tgcccgagga ggtgtccgac gacctggtgg cccgggagat cgaggccgtc
     2581 gacggcgtcg acgtcgagga gctgcggtcc gtcgagttcg tcgagcgcga tcctcgcatc
     2641 gacgccttgc aggccgccgt cgccttgaac caggccgacc ccgggctgga gttggccgcc
     2701 gagctggcca gtcgggcaaa gctggagctg cgggcggtgt gggcggcggt ggtcggcccc
     2761 gagggtgtgg aggccgtcgt gggcgtggca ccggaggcgg cctggctctc ggcgttcggc
     2821 gccggcgtgg ccgcgggcgg acggggcgat gccggcgtgg ccgcggacga agacggtgtg
     2881 gtggcctggg cggcggcggg cgagggacgg gtcctgctca ccggtcggag cggcgtgccg
     2941 ttccgccccc gcgaacgacg ggtcctcggc ctgctgggcg acctggccgc gacccgctga
     3001 ccgcggaggt tcatcgaccg ttcacccgga tgctgcttgc tcgagcgcag tatccgctac
     3061 ctttggtaag catgatggcg gtgtcgggca cccacgtgcc gcccaccgag gcggccgatc
     3121 cctcaccggt tcaccgcgcc ttcgggttct ccgacatctg cggcttcacc gcctacctcg
     3181 agcgggaagg gcccctcgcc gccgtccggc tggtgtcggc gttccgcagc tcggcccgcg
     3241 agatcgccgc tcgccggggc gtgcgcgtgg ccaagtggct gggtgacggg gtcatgttcg
     3301 tctcggtcga agccgggccg cagctggcca cctccgtcga gttgaccaac cgcatggcca
     3361 ccaccgggct ccacctccgt tccggcgtgg ccacgggctc gtgcctgctc atggaggccg
     3421 acgactacat cggccactcg gtcaacctgg cggcccgcct ctgtgacctg gccggccccg
     3481 gtgaggtcct ggccgacgag aacacggcga ccctcgcccc cgactgggtc gaggtcggcc
     3541 caccccgccg caagcgcctg cccggcgtcg gcaaggtcga gggcatctgc agcctccgcc
     3601 tcgacgccga cctcccgctc ctgaccgcct gagacccgtc cgccgcctcg gcctgacgtg
     3661 acaggctccc ggggtgctgt acatggtggt ggagcggtac ctgcggggcc cgggcccggt
     3721 ctattcacgg gtcgaggcga aggggcggat gctccccgac gggctgcgtt acgtcgacag
     3781 ctgggtcgtc gatgacgtcc ccatggaccg gtgcttccag ctgatggaga ccgacgaccc
     3841 gtcgatcctc gacgactgga tggccctctg gtccgacctc accgagttcg aggtgtatcc
     3901 ggtgctgagc tcctccgaag ccgccctgcg cgccctggac tgacccacgg cagtcagccg
     3961 gcttgcttgg ccacggcgtc ggcagcggcc cgcgcggccg ccgcgccacc gaggtagcgg
     4021 tcctccagcg gcacgtcacc aacgggatgg aggtcggcgt ccaactcgta gaccaacggg
     4081 atgccggttg ggatgttgac ctcggtgatg gcggcgtcgg cgatgccgtc gaggtgcttg
     4141 accagggctc gcagggagtt gccgtgggcg gccagcaaca cgacgcgacc ggcccggagg
     4201 tcgggcacga cggcgtcgta ccagtagggc agcatccgca cgacgacgtc cttgaggcac
     4261 tccgaggccg ggatcagctc gggggccagg tcggcgtagc gggggtcgaa gcgagcgtgg
     4321 cgctcgtcgt cccggtccag cgggggcggg ggcgtgtcgt aggagcgacg ccagatcttc
     4381 accttctcgg cgccgtgctc ctcggcgacc tgcttcttgt ccttgccctg caggtcgccg
     4441 tagtggcgct cgttcaggcg ccagtggcga cggacgggga gccagtcccg gcccagggcg
     4501 tccagggcca gctggccggt gtggatggcc cggcgctgca gggacgtgtg gaccacatcg
     4561 ggcaggatcc cggcctcggc cagctgctcc ccaccccgca gggcctcggc ccggcccagc
     4621 tcggtcaggt cggcgtcgta ccacccggtg aacaggttgg cctcgttcca cgtgctctgg
     4681 ccgtggcgca gaagaatcag tcgggaagtt gataacgaag gactcaagtt tctatactca
     4741 gcagtcgaca atcggtcgcg aaccccagaa accccagcaa tccaggagcg tacccccatg
     4801 cccaaggccg tcggaatcga tctcggtacc accaactccg tcgtcgccgt cctcgaggcg
     4861 ggcgaagcca cggtgatccc caacgccgaa gggtcccgca cgaccccgtc ggtcgtcgcc
     4921 ttcgccaagg acggcgaggt cctcgtcggc gaagtggcca agcgccaggc catcaccaac
     4981 cccgaccgca ccgtccgctc ggtcaagcgg cacatgggca cgagctggtc gatcgacatc
     5041 gacggcaaga gctacaagtc ccaggagatc gcggcccgcg tgctgcagaa gctcaaggcc
     5101 gacgccgagg cctacacggg cgagaccgtc aaccaggcgg tcatcacggt gcccgcctac
     5161 ttcgacgacg cccagcgcac cgccacccag gaggcgggcc agatcgccgg gctcgaggtc
     5221 ctgcgcatca tcaacgagcc gaccgccgcg gccctggcct acggcatgga caaggagggc
     5281 gaggaccaga ccatcctggt gttcgacctc ggcggcggca ccttcgacgt ctccctgctc
     5341 gaca
//
LOCUS       DASTFV010000408         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1510755, whole genome shotgun sequence.
ACCESSION   DASTFV010000408 DASTFV010000000
VERSION     DASTFV010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510755"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1371)
                     /gene="rodA"
                     /locus_tag="VFP02_07555"
     CDS             complement(205..1371)
                     /gene="rodA"
                     /locus_tag="VFP02_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HET9542921.1"
                     /translation="MTAPAVARGGLAPSRNPSIRRSFVVPERFEHVDWILVASTVAVA
                     LLGVVMVYSATRGTDDDPTTYFLQRQAMFVVIGVGLMAAMAAFDYRRLRDWAIPIAGA
                     ALFLLFAVLTPLGTESKGIQAWFEVAGFQLQPAELAKLALIIGLAAFLTLEKEDIDGK
                     RLATILGISAVPMALIMLQPDLGTVLVFGVMSLAIVFVAGARPRHFAVLLLIGIIGTI
                     GILNSNVLADYQQDRLTNFANPELNADGSGYNQQQSQTAISAGQLRGQGLFEGTQTGL
                     GFVPEQHTDFIFTVVGEELGFVGGAVVLGLLSVILWRVWRTAQLARDEFGMLVCVGVL
                     AMLTFQMFENIGMTMGIMPVTGIPLPFMSYGGSSILTAFAAIGLVLNVHIHRFG"
     gene            complement(1368..>2443)
                     /locus_tag="VFP02_07560"
     CDS             complement(1368..>2443)
                     /locus_tag="VFP02_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9542922.1"
                     /translation="LSNKVISGVYAPGSTFKPVTALAGLRSGLVTPGDTFNDDGVYEV
                     VGCEDQPSCQFQNAGETANGTVDLQRSLTVSSDWYYYRIGDNLWGNKDSLGESYLQDT
                     AVDWGFTEDTGIDLPQEQNGFAYTPERIAELHEENPAAYPFGDWHTGNTVNMAIGQGD
                     LGVTPMQLANMYAALANGGTLYRPHVGMATLRPVPGTWQEDPGTPCVANDGTVDEGAL
                     CTVDPTQVGAVEIPSRWRDPMIAGLLGVAQSSQGGTSGPAFDGFDLAGFPIAGKTGTA
                     QVYGKGDSALYVGFGPVRAGQNPEYVIAVIYEDVDQFGGEVAAPVARAVFDGLSNQVP
                     LPLVETVPATPEPSLDVQTEADP"
BASE COUNT          382 a          818 c          877 g          366 t
ORIGIN      
        1 gacctcgtag gcgtcggggt agagcaggag ccaggccacc cggccggggc cgtgctcggg
       61 ggttatcgcc cccagctcgc ccccgatgta gcgggccggc ttctggacct cggccaggag
      121 cggctccagc tcgggccaca tggtcatgat cgctcggagt ctaccgggac gggggtgtgg
      181 gccggaggta ccgcgcccct gcgttcagcc gaagcggtgg atgtggacgt tgaggacgag
      241 gccgatggcc gcgaacgcgg tgaggatcga cgaaccgccg taggacatga acggcagagg
      301 gatgcccgtc accggcatga tccccatggt catcccgatg ttctcgaaca tctggaacgt
      361 caacatggcc aagaccccga cgcagacgag catgccgaac tcgtcccggg ccaactgggc
      421 cgtccgccag acccgccaga ggatcacgga cagcaagccg agcaccaccg cgcctcccac
      481 gaagccgagc tcctctccca ccaccgtgaa gatgaagtcg gtgtgctgct cgggcacgaa
      541 gcccagcccg gtctgcgtgc cctcgaacag gccttggccg cggagctggc ccgcgctgat
      601 ggccgtctgg gactgttgct ggttgtaccc gctcccgtcg gcgttgagct cggggttggc
      661 gaagttggtc aaccggtcct gctggtagtc ggccaggacg ttgctgttca ggatcccgat
      721 ggtcccgatg atcccgatga gcagcaggac ggcgaagtgc cggggccggg cgccggccac
      781 gaagacgatc gccaacgaca tgacgccgaa gaccagcacc gtgcccaggt cgggctgcag
      841 catgatcagc gccatcggca cggccgagat gccgaggatg gtggccaggc gcttgccgtc
      901 gatgtcctcc ttctccaggg tgaggaaggc cgccagcccg atgatcaggg ccagcttggc
      961 gagctcggcg ggctggagct ggaagccggc cacctcgaac caggcctgga tgcccttgga
     1021 ctcggtgccg agcggggtga ggacggcgaa gaggaggaag agggcggccc cggcgatggg
     1081 gatcgcccag tcccgcagtc ggcggtagtc gaaggccgcc atggcggcca tcagcccgac
     1141 gccgatgacc acgaacatgg cctggcgctg gaggaagtag gtggtggggt cgtcgtcggt
     1201 ccccctggtg gcgctgtaga ccatcaccac cccgagcagg gccacggcca ccgtggacgc
     1261 caccaggatc cagtccacgt gctcgaagcg ctcgggtacg acgaacgacc ggcggatcga
     1321 cgggttccgg gacggggcca ggccgccccg ggccacggca ggggcggtca cgggtccgcc
     1381 tcggtctgga catcgagtga cggctcgggc gtggcgggga ccgtctcgac gaggggcagg
     1441 gggacctggt tgctgagccc gtcgaagacg gcccgggcga cgggcgccgc cacctcacca
     1501 ccgaactggt cgacgtcctc gtagatgacg gcgatcacgt actccgggtt ctgacccgcc
     1561 cggaccggcc cgaacccgac gtagagggcc gagtcgccct tgccgtagac ctgcgccgtc
     1621 ccggtcttcc ccgcgatcgg gaagcccgcc aggtcgaacc cgtcgaaggc cgggcccgac
     1681 gtcccgccct ggctgctctg ggcgacgccc agcaggccgg cgatcatcgg gtctcgccac
     1741 cgggaaggga tctcgacggc accgacctgg gtcgggtcga cggtgcagag cgccccctcg
     1801 tcgacggtgc cgtcgttggc cacgcacggc gtccccgggt cttcctgcca ggtgcccggg
     1861 accggtcgga gcgtcgccat cccgacatgg ggtcggtaga gcgtcccgcc gttggccagg
     1921 gcggcgtaca tgttggccag ctgcatcggc gtgacgccga ggtcgccctg tccgatggcc
     1981 atgttgaccg tgttcccggt gtgccagtcc ccgaagggat aggccgcggg gttctcctcg
     2041 tggagctcgg cgatgcgctc cggggtgtac gcgaacccgt tctgttcctg gggcaggtcg
     2101 atgccggtgt cctcggtgaa cccccagtcg accgccgtgt cctgcaggta gctctcaccg
     2161 agcgagtcct tgttgcccca gaggttgtcg ccgatgcggt agtagtacca gtcactcgaa
     2221 accgtgaggg acctctggag atccaccgtc ccgttcgccg tctcgccggc gttctggaac
     2281 tggcaggacg gctggtcctc acagcccacg acctcgtaca cgccgtcgtc gttgaaggtg
     2341 tcgcccgggg tgacgaggcc cgaccggagc ccggcgagcg cggtgacggg cttgaaggtc
     2401 gaccccgggg cgtagacccc ggagatgacc ttgttgctca gcc
//
LOCUS       DASTFV010000409         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1512421, whole genome shotgun sequence.
ACCESSION   DASTFV010000409 DASTFV010000000
VERSION     DASTFV010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512421"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VFP02_07565"
     CDS             <1..1146
                     /locus_tag="VFP02_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542923.1"
                     /translation="QARGGATVVGARSAAWAPVVDLAAVLVLDEHDEAHKQEQTPAWH
                     ARDVVVERARRAGVPCVLASPCPSLEALEAGRLVTQPRSVEREGWPIVDVIDRRQDEP
                     GRAGLYSPRLVELMRGGGRVLCVLNRKGRARLLACAACGEMAGCERCGAALGQTEPGT
                     LTCLRCDLSRPTVCAACGGTRMKLLRVGVTRAAEELAALARTEVVEVTGESAPASLSE
                     ARIYVGTEAVLHQVPQADVVAFLDLDQELLAPRYRAAEEALALLARASRILGGRRRDG
                     RLVLQTRLPDHEVVQAALHADPGRVSAAERERRRILGYPPSTAMAAVSGAAADEYVLG
                     VEGIDLLGPRDGTWLLRAPDHTTLLSALEQAPRPAGRLRLDVDPLRV"
     gene            1172..1729
                     /gene="rsmD"
                     /locus_tag="VFP02_07570"
     CDS             1172..1729
                     /gene="rsmD"
                     /locus_tag="VFP02_07570"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET9542924.1"
                     /translation="MRIIGGSARGRKLVGPSSEGTRPLSDRARESLFNILGPGIRGER
                     FLDLFAGTGAVGLEALSRGALSATFVEQDRRALGDIQANLNTLGFADQGSAVAGDALG
                     FIRRTRESFDVIFAGPPQWAALWKATIEAIDTRPEILADGGLLVTQLDPSEDIGDPEL
                     ARLERVDQRAYGKTLLLFHEVRPPD"
     gene            1812..>2052
                     /locus_tag="VFP02_07575"
     CDS             1812..>2052
                     /locus_tag="VFP02_07575"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HET9542925.1"
                     /translation="MEAAAGDLVGLHSSDPATVVLSARARRPEATVEELEDALSETRP
                     LVRLLGMRRTRFVVPRDLAGVIDVSCARDLARAERK"
BASE COUNT          237 a          753 c          780 g          282 t
ORIGIN      
        1 caggcccggg gtggggcgac ggtcgtcggg gcccgatcgg ccgcctgggc ccccgtcgtc
       61 gacctggccg ccgtgctcgt gctggacgag cacgacgagg cccacaagca ggagcagacc
      121 ccggcctggc acgcccggga cgtggtcgtc gagcgagctc ggcgggccgg cgtgccctgc
      181 gtgctggcct cgccgtgccc gtcgctggag gccctcgagg cggggcggct ggtgacccag
      241 ccccgctccg tcgagcggga ggggtggccg atcgtcgacg tcatcgaccg ccggcaggac
      301 gagcccgggc gggccgggct ctactcgccc cgcctggtcg agctgatgcg ggggggcggg
      361 agggtgctct gcgtactcaa ccgcaagggc cgggcccgcc tgctggcctg tgccgcgtgc
      421 ggggagatgg ccggctgcga gcggtgcggt gcggcgctcg gccaaaccga gccggggacg
      481 ctgacctgcc tgcgctgtga cctcagccga ccgacggtgt gcgcggcgtg cggtggcacc
      541 cggatgaagc tgctgcgggt gggggtgacc cgggcggccg aggagttggc ggcgctggcc
      601 cggaccgagg tggtggaggt gacgggggag tcggcgcccg cctccctctc cgaggcccgc
      661 atctacgtgg gcaccgaagc cgtgctccac caggtgccgc aggccgacgt cgtcgccttc
      721 ctggacctcg accaggaact gctggctccc cggtaccggg cggccgagga ggcattggcg
      781 ctgctggcgc gcgcctcccg gatcctgggc gggcggcgtc gggacggacg gctggtcctc
      841 cagacccgcc tgcccgacca cgaggtcgtg caggccgccc tccacgccga ccccggtcgg
      901 gtcagcgccg ccgagcggga acggcgtcgg atcctggggt accccccgtc gacggccatg
      961 gccgcggtga gcggggcggc cgccgacgag tacgtcctcg gggtggaggg catcgacctc
     1021 ctgggcccga gggacggcac ctggctcctg cgggccccgg accacacgac cctgctttcg
     1081 gctctcgagc aggccccccg tcctgccggc cgcttgcgcc tcgacgtcga ccccctccgc
     1141 gtctgatgcc tcccctggca aggtggttgg cgtgaggatc atcggaggct cggcccgcgg
     1201 ccggaagctg gtcgggccgt cctcggaggg gacgcggcct ctgtcggatc gggctcggga
     1261 gtcgctcttc aacatcctcg gccccggcat ccggggcgag cggttcctcg acctgttcgc
     1321 gggcaccggc gccgtgggcc tcgaggccct gagccggggg gccctctcgg ccaccttcgt
     1381 cgagcaggac cgccgggccc tgggtgacat ccaggccaat ctgaacacgc tgggcttcgc
     1441 cgaccagggg agtgcggtcg cgggcgatgc cctcggcttc atccgccgga cccgggagtc
     1501 cttcgatgtg atcttcgccg gtccacctca gtgggcggcc ctgtggaagg ccacgatcga
     1561 ggccatcgac acccgccccg agatcctcgc cgacgggggc ctgctggtga cccaactgga
     1621 cccctccgag gacattggcg accccgagct cgcgcgcctc gagcgggtgg accagcgcgc
     1681 ctacggcaag accctcctgc tgttccacga agtccgccca ccggactgat cgcgtgcgcc
     1741 gtcgcttcga cgatgacgaa cggcgagccc gactcggccg tcgtcaccac ctcagccggc
     1801 cagccccctc ggtggaggct gcggccggcg acctggtggg gctgcactcc tcggatcccg
     1861 ccacggtggt gctgtcggcc cgggcccgcc gccccgaggc cacggttgag gagctcgagg
     1921 acgcgctctc cgagacgcga ccgctcgtgc gcctgctggg catgcgccgg accaggttcg
     1981 tggtgccccg ggacctggcc ggcgtgatcg atgtcagctg cgctcgggac ctggcccggg
     2041 ccgagcgcaa gc
//
LOCUS       DASTFV010000410         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1523180, whole genome shotgun sequence.
ACCESSION   DASTFV010000410 DASTFV010000000
VERSION     DASTFV010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523180"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1673
                     /locus_tag="VFP02_07580"
     CDS             105..1673
                     /locus_tag="VFP02_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HET9542926.1"
                     /translation="MSDEKERRYPGTWAERRPDHPAVIMGGTGEVVTYAELHDTACRL
                     AQLFKSLGLQPGDHMALCLENRPEYLPLLWGARYAGLYYTAISSRLTAEELGYIVEDS
                     GGRVLITSPAKADQVAELMGQLGGLDLLASIGGPVVGCEPLEGLLAAQPAEEPADAVE
                     GHAMLYSSGTTGRPKGVKALMAGDPLGTPDALTTFNTLVFGASEASVYLSPAPLYHAA
                     PLGFSMAFLRLGATVVVMEHFDAAEALALIERHQVTHSQWVPTMFVRLLKLPEEVRSA
                     PDLSSLKVAVHAAAPCPIPVKEQMIEWWGPVIHEYYAGTEGNGFCYCNSEDWLAHKGT
                     VGRPLTAPVLILDEEGREVATGQEGTIWFGLTAAGRIFEYHNDPDKTAGSYREDGSST
                     LGDIGRVDEDGFLYLTDRKANMIISGGVNIYPQETENVLIVHPDVADAAVIGVPNEEF
                     GEEVKAVVQLVDPSAAGPEMARALIAHCRDHLADIKCPRSVDFRDELPRHPTGKLYKR
                     LLKDEYWAGHDSRI"
     gene            1716..1907
                     /locus_tag="VFP02_07585"
     CDS             1716..1907
                     /locus_tag="VFP02_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542927.1"
                     /translation="MPFVVTFGLSTAVVGGICGYLFGKRWKNRPVLGAVLCFVIPILG
                     LIILWYVPPKAPGPMRPAT"
BASE COUNT          333 a          747 c          700 g          305 t
ORIGIN      
        1 gatcaccggc gtccggtcag gccccacgct cacgacgcgg gaccgtagcc gggccagaat
       61 gggcgccgga acggcggaca ccaagcgacc ggggggcgac gcgcgtgagc gacgagaagg
      121 aacggcggta cccggggacg tgggcggagc ggcggccgga ccaccccgcc gtcatcatgg
      181 gcggcaccgg cgaggtcgtc acctacgccg agctccacga cacggcctgc cggctggcgc
      241 agctgttcaa gtccctcgga ctgcagcccg gtgaccacat ggccctgtgc ctcgagaacc
      301 gtcccgagta cctgccgctc ctgtggggcg cccggtacgc cggcctctac tacacggcca
      361 tcagctcgcg gctgacggcg gaggagctgg gctacatcgt cgaggactcg ggcggccggg
      421 tcctcatcac gtcaccggcc aaggccgacc aggtggccga gctgatgggc cagctcggtg
      481 gactcgacct cctggcgtcg atcggcggcc ccgtcgtcgg ctgcgagccg ctggagggcc
      541 tcctggctgc tcaacccgcc gaggagcccg ccgacgccgt cgagggccac gccatgctct
      601 actcctcggg caccaccggg cggcccaagg gcgtcaaggc cctgatggcc ggcgacccgc
      661 tggggacccc cgacgccctg accaccttca acacgctggt cttcggcgca tcggaggcgt
      721 cggtctacct ctcccccgcc cccctctacc atgccgcccc gctcggcttc agcatggcct
      781 tcctgcgcct gggggcgacg gtcgtggtca tggagcactt cgacgccgcc gaggccctgg
      841 ccctcatcga gcgacaccag gtgacccaca gccagtgggt gcccaccatg ttcgtgcgct
      901 tgctcaagct gcccgaggag gtccggtccg cgcccgacct cagctcgctg aaggtggctg
      961 tccacgccgc cgccccctgt ccgatcccgg tcaaggaaca gatgatcgag tggtgggggc
     1021 cggtcatcca cgagtactac gcgggcaccg agggcaacgg cttctgctac tgcaacagtg
     1081 aggactggct ggcccacaag gggacggtcg gacgacccct gaccgctccg gttctcatcc
     1141 tcgacgagga gggccgggag gtcgcgacgg gccaggaggg cacgatctgg ttcggtctca
     1201 cggcggcggg ccggatcttc gagtaccaca acgacccgga caagacggcg ggcagctacc
     1261 gggaggacgg ttcgagcacc ctgggcgaca tcggcagggt ggacgaggac ggcttcctct
     1321 acctcaccga ccgcaaggcc aacatgatca tctcgggcgg cgtgaacatc tatccccagg
     1381 agaccgagaa cgtcctgatc gtgcaccccg acgtggccga cgccgcggtc atcggcgtcc
     1441 cgaacgagga gttcggcgag gaggtcaagg ccgtcgtcca actcgtcgac ccgtcggccg
     1501 ccggcccgga gatggcgcgg gcgctcatcg cccactgccg ggaccacctg gccgacatca
     1561 agtgcccccg ctcggtcgac ttccgcgacg agctcccccg ccacccgacg ggcaagctct
     1621 acaagcgcct cctgaaggac gaatactggg ccggtcacga cagccggatc tgacgtggga
     1681 ggctggctct tccacccgct cgaaagggag cacgcatgcc gttcgtcgtc accttcggtc
     1741 tcagcaccgc ggtggtaggt gggatctgcg gctatctctt cggcaagcgt tggaagaacc
     1801 ggcccgtgct cggtgccgtc ctctgcttcg tgatccccat cctcgggctg atcatcctct
     1861 ggtacgtccc gccgaaggcg cccgggccga tgcggcccgc gacctgaaca caggcccggg
     1921 aaccgggtga cggtcgcctg ggagcacggc cctgatccat cggaggcaac aaggggcggc
     1981 cttcgcccct tattgcctcc aatagttgcc ggcttcgacc ggctactgga acgtgttcta
     2041 gtttgggggc gtgagcacga cgggggaacg caccttcaac ctggc
//
LOCUS       DASTFV010000411         3592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1526693, whole genome shotgun sequence.
ACCESSION   DASTFV010000411 DASTFV010000000
VERSION     DASTFV010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526693"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFP02_07590"
     CDS             complement(<1..478)
                     /locus_tag="VFP02_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9542928.1"
                     /translation="MPRLMAAWYDRSFAKTEAAGLRDWRAALLADLAGEVLEIGPGTG
                     LNLEHYPATVTRLVLAEPDRFMRAKLAAKVEARSAPFPIEIADAGVDPIPFADGSFDV
                     VVSTLVLCSVPDHEAALAEIHRVLRPGGRFVFLEHIAAVENPKRHRWQQRIEPLWKR"
     gene            502..1896
                     /locus_tag="VFP02_07595"
     CDS             502..1896
                     /locus_tag="VFP02_07595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9542929.1"
                     /translation="MPGRGAEVDETGAVAHPYWPLFDIRVRTPRLELRPPTDEDLLAL
                     VDLVRRGIHEPTMMPFSNPWTDTPSPWLERQSLQWNWRQRADWTKEGWGLGFAVVLRD
                     PEPTIVGVQDIFAKNFHLTGVAESGSWLGMEHQGKGIGKEMRAAILHFGFAGLRARWL
                     ETFAWEDNASSLGVTERLGYEPNGVEMDLRRNQGQVLRRYRMTRETWEQQRRRDIRIE
                     GLERCLDMFGLPQAVAGADACRGGWCVVTLPVDGDATVEVVDRFTKVATRQAKGQLGA
                     VGVDIPIGLSDGPPRQCDIEARALLGDRRSSVFAAPVRSVLGARSFEQALSRSRSAGG
                     VGLSKQSWNLVPKVGEVDRLLDPTRQGSIFECHPEAAFAAVHGVPMAHPKKSRPGRDE
                     RLATLRPHVPGIDALVAKPPGGAAVDDVLDAAVVALTARALLAGKARRLGERRHDAKG
                     LVMEIVIPEAGAFP"
     gene            1964..3577
                     /locus_tag="VFP02_07600"
     CDS             1964..3577
                     /locus_tag="VFP02_07600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HET9542930.1"
                     /translation="MLRLTGLDAGFLSMETPTAPMHVAGLAIFDPSEVEGRFDVDHLR
                     EVYEPRLHLAPPFRRRLREVPFGLHHPLWIEDPDFDIDWHLRHVAVPAPGGPRELAEL
                     AAHLNAIPLDRSRPLWEMWLIDGVAGGHVAVLSKVHHAAIDGASGNEITVATLDIAQE
                     GSEVPPPEVEWTPDAAPSDVSMLTHATLSLLASPVGVVRAARRSIDAALGRRSLPRSS
                     SPPAPFAAPKTSVNGPLTRQRVFAMTSLSLPAVKAIKVHHDVKVNDVVLALCAGALRR
                     WFEDRGEDLSAPLVAMVPVSVRTEGEKGAMGNRVSSMLVSLATDVSDPALRLQVIGDA
                     TREAKERHAVGADALTDWAEFAAPALLGRAARLYSRMHLANLHRPVFNVTISNVPGPP
                     FPLYSAGARLAANYPMGPIFDGAGLNMTVMSYQDQLDFGLQGDPALLGDPWVLAGFLE
                     DALAELAATVPELDGVALVTQPFGAAVASPAPAALTSEDLTVIEDTDELITRAHEALG
                     RAHADRADAAAIKAALDQLRRAADFTQPA"
BASE COUNT          494 a         1354 c         1244 g          500 t
ORIGIN      
        1 agcgcttcca cagaggctcg atgcgctgtt gccagcggtg acgcttgggg ttctcgacgg
       61 cggcgatgtg ctcgaggaag acgaagcgcc cgcccgggcg caggacccgg tggatctcgg
      121 ccagcgccgc ctcgtggtcc gggacggagc agaggacgag ggtcgacacg accacgtcga
      181 agctgccgtc cgcgaacggg atgggatcga cgccggcgtc ggcgatctcg atcgggaacg
      241 gggccgaccg ggcctcgacc ttcgcggcca gcttggcccg catgaaccgg tccggctcgg
      301 ccaggacgag gcgggtcacc gtggccgggt agtgctccag attgagcccg gtgccggggc
      361 cgatctcgag gacctcgccg gccaggtcgg ccagcagggc ggcccgccag tcccgcaggc
      421 cggccgcctc ggtcttggcg aacgaccggt cataccacgc agccatgagt cggggcacgc
      481 cgggactgta ggcctgaaac catgcccggt cggggggccg aggtcgacga gactggcgcc
      541 gtggcgcacc cctattggcc gttgttcgac atccgggtgc gcacgccccg cctcgagttg
      601 cgaccaccga cggacgagga cctgctggcg ctggtcgacc tggtccgccg cgggatccac
      661 gagccgacga tgatgccgtt ctccaacccg tggaccgaca cgccgtcgcc gtggctggag
      721 cgccagtcgt tgcagtggaa ctggcggcag cgggccgact ggacgaagga gggctggggt
      781 ctgggcttcg ccgtcgtgtt gcgcgacccc gagcccacga tcgtcggcgt ccaggacatc
      841 ttcgccaaga acttccacct caccggtgtg gccgagagcg gctcgtggtt ggggatggag
      901 caccagggca agggcatcgg caaggagatg cgggcggcca tcctccactt cggcttcgcc
      961 gggctccggg cccgctggct cgagaccttc gcctgggagg acaacgcctc ctccctcggc
     1021 gtcaccgagc gcctcggcta cgagcccaac ggcgtggaga tggacctccg gcggaaccag
     1081 ggccaggtcc tgcgccgcta ccggatgacc cgggagacat gggagcagca gcgacgccgc
     1141 gacatccgca tcgagggcct cgagcgctgc ttggacatgt tcggccttcc gcaggccgtc
     1201 gccggggccg acgcctgccg ggggggctgg tgtgtcgtga ccctgcccgt cgacggtgac
     1261 gccacggtcg aggtcgtcga ccgcttcacg aaggtcgcca cccgccaagc caagggccag
     1321 ctcggcgcgg tcggcgtcga catcccgatc ggcctgtccg acggcccgcc ccgccagtgc
     1381 gacatcgaag cccgggccct gctgggcgac cgccggtcat cggtcttcgc cgccccggtc
     1441 cggtcggtgc tcggggcccg gtccttcgag caggccctca gccggtcccg gtcggccggc
     1501 ggggtcggcc tgtcgaagca gtcgtggaac ctcgtgccca aggtcggcga ggtcgaccgc
     1561 ctgctcgacc cgactcgcca gggcagcatc ttcgaatgcc atcccgaggc tgccttcgcc
     1621 gcggttcacg gcgtcccgat ggcgcacccc aagaagagcc ggccgggccg ggacgaacgc
     1681 ctggccaccc tccggccaca cgtccccggc atcgacgccc tcgtggccaa gccgccgggc
     1741 ggggctgcgg tcgacgacgt cctcgacgcc gctgtggtcg ccctcacggc ccgcgccctg
     1801 ctggccggaa aggcgcgtcg cctcggcgaa cgccggcacg acgccaaggg cctggtcatg
     1861 gagatcgtca tcccagaggc aggcgccttc ccgtagtggt actcctgagg gccaccggcc
     1921 gacggtggtc gtaccatcgg cccacacgac cagggggtgt cgcatgctgc ggttgactgg
     1981 cctcgacgcc gggttcctct ccatggagac gcccaccgcc ccgatgcacg tggccgggct
     2041 ggccatcttc gacccgtccg aggtcgaggg ccgtttcgac gtcgaccacc tgcgtgaggt
     2101 ctacgagccc cgcctccacc tggccccgcc cttccgccgc cgactgcggg aggtgccctt
     2161 cggcctccac catccgctgt ggatcgagga ccctgacttc gacatcgact ggcacctgcg
     2221 ccacgtggcc gtccccgctc ccggagggcc gcgggagctg gccgagctgg ccgcccacct
     2281 caacgccatc cccctcgacc gcagccgccc gctgtgggag atgtggctga tcgacggcgt
     2341 ggccggcggg cacgtggccg tgctcagcaa ggtccaccac gccgccatcg acggggcatc
     2401 cggcaacgag atcaccgtgg ccaccctcga catcgcccag gaaggcagtg aggtcccacc
     2461 ccctgaggtg gagtggacgc ctgacgccgc cccgtccgac gtctcgatgc tgacccacgc
     2521 caccctctcg ctgctggcct cgccggtcgg cgtggtgagg gccgcccgcc gcagcatcga
     2581 cgccgccctg gggcggcgga gcctgccccg ctccagctcg ccgcccgcgc cgttcgccgc
     2641 ccccaagaca tcggtcaacg gccccctcac ccgccagcgg gtgttcgcca tgacctcctt
     2701 gtccctgccg gcggtcaagg ccatcaaggt ccaccacgac gtgaaggtca acgacgtcgt
     2761 cctggccctc tgcgccggtg ccctgcggcg ctggttcgag gaccggggcg aggacctgtc
     2821 cgcaccgctc gtggccatgg tcccggtgtc ggtgcggacc gagggggaga agggggccat
     2881 gggcaaccgg gtgtcctcga tgttggtgag cctggccacc gacgtcagcg acccggccct
     2941 gcggttgcag gtgatcggcg acgccacccg ggaggccaaa gaacgccacg ccgtcggcgc
     3001 tgacgcgctg accgattggg ccgagttcgc ggcgccggcg ctgttgggcc gggccgcccg
     3061 cctctactcc cgcatgcacc tggccaacct gcaccggccc gtgttcaacg tgaccatctc
     3121 caacgtcccc ggcccgccgt tcccgctcta ctcggccggg gcccggctgg ccgccaacta
     3181 cccgatgggg ccgatcttcg acggggccgg cctgaacatg accgtcatga gctaccagga
     3241 ccagctcgac ttcggcctcc agggcgatcc cgcgctgctg ggcgacccgt gggtcctggc
     3301 ggggttcctc gaggacgcct tggccgagct ggccgccacc gtccccgagc tggacggggt
     3361 ggcgttggtg acccagccct tcggcgccgc ggtcgccagc ccggcgccgg ccgccttgac
     3421 gagcgaggac ctgaccgtga tcgaggacac cgacgagctg atcacccggg cccacgaggc
     3481 cctcggccgg gcccacgccg accgggccga cgccgccgcc atcaaagccg ccctcgacca
     3541 gctccgccgc gccgccgact tcacccaacc cgcctgacgc gttctgatcc ac
//
LOCUS       DASTFV010000412         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1526930, whole genome shotgun sequence.
ACCESSION   DASTFV010000412 DASTFV010000000
VERSION     DASTFV010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526930"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..845)
                     /locus_tag="VFP02_07605"
     CDS             complement(111..845)
                     /locus_tag="VFP02_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9542931.1"
                     /translation="MSGGSALAAQARIETTLSLRRGESLLLTLGIPVGLLVFFCVVDV
                     LPIDGDDPVAFLAPGVLALAVMSTAMVNLAIATGFERQYLVLKRLGATPLGRGRLLGG
                     KTIAIGLVLVVQVVVLTVVALLLGWDPDVAGIPLAFAGAVLATVAFAGIGLWMAGTLR
                     GEMTLALANGLYLVLLLLGGMIIPLDELPRAVRTFAELLPAAALADIMTGALTPGGDI
                     AARSWLVLVVWAAAAPAAAVRWFRWE"
     gene            complement(842..1756)
                     /locus_tag="VFP02_07610"
     CDS             complement(842..1756)
                     /locus_tag="VFP02_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9542932.1"
                     /translation="MSEPSAVEVRDLRIAYGDVVAVDGVSFAAEAGEVVALLGPNGAG
                     KTSTIEALEGFRRPAAGAVRVLGHDPLADHAAVVGCIGVMLQEGGLYPGIRTAEVLDL
                     FAAYYDDPADPAALLRRVGLEDQGKATARRLSGGQQQRLSLALALVGRPQVAFLDEPT
                     AGLDVGGRLLVRDVIREQRDAGVCVLLATHDLAEAEKVADRVVIVDRGRVVADGTPEE
                     LMSAPAAEIRFGAPAGLDVHSLATALGAPVTEETDGEYRVATEPSPRTVAALTAWLAE
                     RNLALGDLRAGRQHLEDVFLRLTRENAP"
     gene            1841..>2675
                     /locus_tag="VFP02_07615"
     CDS             1841..>2675
                     /locus_tag="VFP02_07615"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HET9542933.1"
                     /translation="MIDVRLIRSDPDAVKSALGRRGEDLTPIDRVIELDRRQRDLTQE
                     RDEIRHQVKALSKEVGGLREAGETEQAEALQAESRALGEREAALAEEADARAEEVRQI
                     LLLVPNLPSPDAPDGASEGDNPVLRQGGPDPDNYAEHQQVPHWETGETLGILDFERGV
                     KLAGSMFVAYRGLGATVARALCQLALDRNADLYEEVRPPSLVRTEIMVGTGQLPKFHE
                     DAYHLERDDLWAVPTAEVPLTSLYRDEILDEDDLPMRVRAYAPCFRREAGAAGRAPRG
                     LL"
BASE COUNT          392 a          965 c         1015 g          303 t
ORIGIN      
        1 cgccggcgtc caggttcagg tacggacggt cgacgccgtc gacgcagggc acgggctcgc
       61 cgtcgccgac cagggtgacg ggcaggcggg gggcgttttt cacggcggtc tcactcccat
      121 cggaaccagc ggacggccgc cgccggggcg gcggcggccc acacgacgag cacgagccag
      181 gaccgggcgg cgatgtcgcc cccgggggtg agcgccccgg tcatgatgtc ggccagcgcg
      241 gcggcgggca gcagctcggc gaacgtccgc acggcgcggg gcagctcgtc gagggggatg
      301 atcatgccgc ccagcaggag caggaccaga tagaggccgt tggccagggc cagggtcatc
      361 tcgccccgca gcgtgcccgc catccagagg ccgatcccgg cgaaggccac ggtggcgagg
      421 acggcgccgg cgaaggccag ggggatgccg gcgacgtcgg ggtcccagcc caggaggagg
      481 gccaccacgg tcaggaccac cacttgcacg acgaggacca ggccaatggc gatggtcttg
      541 ccgccgagga ggcgccctcg gcccagaggg gtggccccca ggcgcttgag gacgaggtac
      601 tggcgttcga acccggtggc gatggccagg ttgaccatgg cggtggacat gacggcgagg
      661 gccaacaccc ctggggcgag gaaggccact gggtcgtcgc cgtcgatggg cagtacgtcg
      721 acgacgcaga agaacacgag caggccgacc gggatgccca gggtgagcag cagcgactcg
      781 ccccgccgca gggacagcgt ggtctcgatg cgggcctggg cggccagcgc gctccctccg
      841 ctcacggtgc gttctcccgg gtgagtcgga ggaagacatc ctcgaggtgc tggcggccgg
      901 cccggaggtc gcccagggcc aggttgcgct cggccagcca ggccgtgagc gcggccacgg
      961 tgcgggggga gggctcggtc gccacccggt actcgccgtc ggtctcctcg gtgaccgggg
     1021 cgccgagcgc ggtggccagg ctgtgcacgt ccagcccggc gggggcaccg aagcggatct
     1081 cggcggccgg ggcgctcatc agctcctccg gcgtgccgtc ggcgaccacg cggccccggt
     1141 cgacgatcac gacccggtcg gccaccttct cggcctcggc caggtcgtgg gtggccagca
     1201 ggacgcagac cccggcgtcc cgctgctccc tgatcacgtc ccgcaccaac aggcggccgc
     1261 ccacgtcgag accggcggtg ggctcgtcca ggaacgccac ctggggccga ccgacgaggg
     1321 ccaacgcgag ggacaggcgt tgctgctggc cccccgacag gcggcgggcc gtggccttgc
     1381 cctggtcctc cagccccacc cgccggagca gggcggcggg gtcggccggg tcgtcgtagt
     1441 aggccgcgaa gagatcgagg acctcggcgg tgcggatgcc ggggtagagg ccgccctcct
     1501 ggagcatcac gccgatgcaa ccgaccacgg cggcgtgatc ggccaggggg tcgtgaccca
     1561 ggacccggac ggcgcccgcc gccggccggc ggaagccctc gagggcctcg atggtggagg
     1621 tcttgcccgc cccgttcggc cccaggaggg cgaccacctc gcccgcttcg gcggcgaacg
     1681 acaccccgtc cacggccacc acgtcgccgt aggcgatgcg caggtcccgg acctcgaccg
     1741 ccgacggttc gctcacgacc ggacgctacc gactggcccc gggacgggcg caggccgaaa
     1801 ggcccgggtt cgaggggtgg gcggcggtac cttccctgcg gtgatcgacg tccgcctgat
     1861 ccgcagcgac cccgatgcgg tcaagtccgc cctcgggcgc cggggcgagg acctcacgcc
     1921 gatcgaccgg gtcatcgagc tggaccgccg gcagcgggac ctgacccagg agcgggacga
     1981 gatccgccac caggtgaagg ccctgtcgaa ggaggtcggt gggctccggg aggcgggcga
     2041 gaccgagcag gccgaagcgc tccaggccga gagccgggcc ctcggcgagc gggaagccgc
     2101 cttggccgag gaggccgacg cccgggccga ggaggtccgt cagatcctcc ttctcgtccc
     2161 caacctgccg tcgcccgacg cccccgacgg ggcgagtgag ggagacaacc ccgttctgcg
     2221 ccagggcgga ccggaccctg acaactacgc cgagcaccag caggtgcccc actgggagac
     2281 gggcgagacc ctcggcatcc tcgacttcga gcggggggtg aagctggccg ggtcgatgtt
     2341 cgtcgcctac cgcggtttgg gtgccacggt cgcccgcgcc ctctgccagc tggccctcga
     2401 ccgcaacgcc gacctgtacg aggaggtacg gcccccctcg ctggtgcgca ccgagatcat
     2461 ggtcggcacc ggtcagctcc cgaagttcca cgaggacgcc taccacctcg agcgggacga
     2521 cctgtgggcc gtccccaccg ccgaggtccc actcacctcg ctctaccggg acgagatcct
     2581 ggacgaggac gacctgccca tgcgcgtcag ggcctacgcg ccctgcttcc gccgggaggc
     2641 gggcgcggcc ggccgggccc cccggggcct gctgc
//
LOCUS       DASTFV010000413         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1536540, whole genome shotgun sequence.
ACCESSION   DASTFV010000413 DASTFV010000000
VERSION     DASTFV010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536540"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VFP02_07620"
     CDS             <1..775
                     /locus_tag="VFP02_07620"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HET9542934.1"
                     /translation="ATDIAFALAVLAIVGSKAPKELKLFLLTLAIVDDIGAIVVIALF
                     YGEGIDLRWLAGTAAVIVVIALMRLASLVHPAWYVLPGVALWAVTFESGVHATIAGVV
                     LGLLTPTGPVAGREVLVELEDRLHPWSSFLVVPVFALANAGVVLDHASLEAAVTGTVA
                     WGVVLGLVVGKPLGIMTASALAVRLRVGVLPAGVALRHLIGLSLIAGIGFTVSLFVAD
                     LAFGGELLGEAKVAILAASVLAAAGGSILVRSTVPKSWD"
     gene            complement(785..1582)
                     /locus_tag="VFP02_07625"
     CDS             complement(785..1582)
                     /locus_tag="VFP02_07625"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET9542935.1"
                     /translation="MSDSTRDKPTAVLGRRYLAYLIDLLVHLAVVAVPFFLLGEKARD
                     SADTTPFADAYRLNPFYAVRIDDQVWLFTKNDLITIGAVSAAFVILVTVIIQGRVGWT
                     IGKALTGLRTVNRDGGPAGILRALGRTILLVIDAIPSWLIPIVGPLIALVTSDNRRLG
                     DLACGTYVVHKSAVGNDPTGDRGPAAWEPRTDDAAPLTTLAEGEALPVGGAAAVGESA
                     PEEATDEKAPAYQPQWDPARQAYLQWDPRKQQWLQYDEAAGAWHPIG"
     gene            complement(1592..>2187)
                     /locus_tag="VFP02_07630"
     CDS             complement(1592..>2187)
                     /locus_tag="VFP02_07630"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET9542936.1"
                     /translation="AVLLFLVLLPGRKGWSPGYLAADLRLQRRDGDRAGIGRALVRTI
                     AWVIDILPGVPLVAYTSASLTRRHQRVGDLLARTYVVDKRTAGRPVDAPVEDDLAAEV
                     LEPERLIEPVPEVVDAPEPEPVVAPAPGPEPVPVAAAVAAGSGPPDGVPPDEPIWDRR
                     QKRYVLWHSEAGRWLEHGATGWAPLDRDGERDTSRRD"
BASE COUNT          264 a          744 c          826 g          353 t
ORIGIN      
        1 ggccaccgac atcgccttcg cccttgccgt gctcgccatc gtgggcagca aggccccgaa
       61 ggagctcaag ctcttcctct tgacgctggc catcgtggac gacatcgggg ccatcgtggt
      121 gatcgccctc ttctacggcg agggcatcga tctgcggtgg ctggccggga cggcggccgt
      181 catcgtggtg attgctctca tgcggctggc ctcactggtc cacccggcgt ggtacgtgct
      241 gcccggtgtg gccctctggg ccgtcacctt cgagtccggt gtccacgcca ccatcgccgg
      301 ggtggtcctc ggactcctca ccccgaccgg cccggtggcg ggccgggagg tgttggtcga
      361 gctggaggac cggctgcacc cgtggtccag cttcctggtc gtccccgtgt tcgcgctggc
      421 caacgccggc gtcgtgctgg accacgccag cctcgaggcc gcggtgaccg ggacggtggc
      481 ctggggtgtg gtcctcggct tggtggtggg caagccgctg gggatcatga cggcctcggc
      541 gctcgccgtg cgcctgcgag tcggtgtcct gccggccggc gtggcgctcc gtcacctgat
      601 cggcctcagc ctcatcgccg gcatcggctt caccgtctcg ctcttcgtgg cggacctggc
      661 gttcgggggc gagctgctgg gcgaggccaa ggtggccatc ctcgccgcct cggtcctggc
      721 ggcggccggt ggatcgatcc tcgtccggtc gaccgtcccg aagtcctggg actgaccgcc
      781 gcggtcagcc gatcggatgc cacgccccgg cggcctcgtc gtactgcagc cactgctgct
      841 tccgggggtc ccactggagg taggcctgcc gggccgggtc ccactggggt tggtacgccg
      901 gggccttctc gtcggtcgct tcctcgggtg ccgactcgcc gacggcggcc gcgccaccca
      961 ccggcagggc ctcgccttcg gccagggtgg tcaacggggc ggcgtcgtcg gtccggggct
     1021 cccacgccgc ggggccgcga tcgcccgtgg ggtcgttgcc cactgcgctc ttgtggacga
     1081 cgtaggtgcc acacgccagg tcgccgaggc gtcggttgtc ggaggtgacg agggcgatca
     1141 gcggcccgac gatggggatc aaccagctgg ggatggcgtc gatgacgagg aggatcgtgc
     1201 gtccgagggc ccgcaagatg cctgccgggc cgccgtcccg gttcaccgtg cgcaggccgg
     1261 tcagcgcctt gccgatggtc cagccgacgc ggccctggat gatgaccgtg accaggatga
     1321 cgaaggcggc cgagacggcg ccgatggtga tgaggtcgtt cttggtgaag agccagacct
     1381 ggtcgtcgat gcggaccgcg tagaacgggt tgagtcggta ggcatcagcg aacggggtgg
     1441 tgtcggcgga gtcgcgagcc ttctcgccga gcaggaagaa gggcacggcc accacggcga
     1501 gatgcacgag caggtcgatg aggtaggcga ggtaccgcct tcccaggacc gcagtcggct
     1561 tgtctcgggt gctgtcgctc acccttcctg tttagtccct ccgactcgtg tctcgttcgc
     1621 catcccggtc cagcggggcc cagccggtgg cgccgtgctc gagccaacgg ccggcctcgg
     1681 agtgccagag cacgtagcgc ttctgccgcc ggtcccagat gggctcgtcc ggcggcaccc
     1741 cgtccggcgg gcccgacccg gcggcgacgg cggcggccac gggcaccggc tcgggacccg
     1801 gtgccggcgc cacgacgggt tcgggttcgg gggcgtcgac gacctcgggc acgggctcga
     1861 tcagcctctc gggctcgagc acctcggcgg cgagatcgtc ctcgacgggg gcgtctaccg
     1921 gtcggcccgc ggtccgcttg tcgacgacgt aggtccgggc caggaggtca cccacgcgtt
     1981 ggtggcggcg agtgaggctg gccgacgtgt aggccaccag gggtacgccg gggaggatgt
     2041 cgatgaccca ggcgatggtc cggaccaggg cccgtccgat cccggcccgg tcaccgtcgc
     2101 ggcgctggag gcggaggtcg gcggccaggt accccgggga ccagcccttg cgtcccggga
     2161 gcaggacgag gaagagcagg accgcaa
//
LOCUS       DASTFV010000414         4147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1537287, whole genome shotgun sequence.
ACCESSION   DASTFV010000414 DASTFV010000000
VERSION     DASTFV010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537287"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFP02_07635"
     CDS             complement(<1..323)
                     /locus_tag="VFP02_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET9542937.1"
                     /translation="MGHAEGVLGGLRVIDASTEVAGPYATKLLADAGADVVKVEGPEG
                     DPLRRWTAAGVDLPADEDSAFFRFLNTSKRAVVGTVDELAAGADVVIGDGGLDVDGLA
                     GAYSHL"
     gene            434..769
                     /locus_tag="VFP02_07640"
     CDS             434..769
                     /locus_tag="VFP02_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET9542938.1"
                     /translation="MAELWTFEMLEITEPVDLTGFDVEASDGSIGKIDEATYEAGAGY
                     VVVDTGFWIFGKKRMIPAGAITDMDLDNRTVRVNLTKDQIKAAPDYEAAQRDDTDYRD
                     RLSDHYGAS"
     gene            complement(779..1498)
                     /locus_tag="VFP02_07645"
     CDS             complement(779..1498)
                     /locus_tag="VFP02_07645"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9542939.1"
                     /translation="MTEGPQRLLDALSSPVRREILWMVWDQELAVGDIAAAFDVTAPT
                     ISQHLTVLRGAGLVTMRAEGNFRRYRARQDAFRGFEALLSHTGRWAPPDRPADDGVTV
                     VESDVVALHVEVDRTPADAFSGFVEPDVYSRWLGVPVTITDGRFACETERGIRVRGIY
                     DVVSPPRLLAMRWDFDAHDVPVPADLRTAYLRVLPTATGSRVDVHQFVNDDVEARDMR
                     DAWTSVLTRFRDGLTAALAAG"
     gene            1577..1888
                     /locus_tag="VFP02_07650"
     CDS             1577..1888
                     /locus_tag="VFP02_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542940.1"
                     /translation="MANFVLAYRGGSVPDSEADQQAAMEAWMGWFGSLGDAVVDGGAP
                     FGASMSRTGDGAPADGADSALTGYSIVSAGSLAEATELAGGCPVLQSGGSVDVYEAMP
                     M"
     gene            complement(1951..2832)
                     /locus_tag="VFP02_07655"
     CDS             complement(1951..2832)
                     /locus_tag="VFP02_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9542941.1"
                     /translation="MGTLDGKVAIITGAGQGLGAAHAKLFAAEGARLVLNDLGDGVED
                     VVAEITASGGEAVANRTSVSDWNAAKEMIEQAVDTYGDLHILVNNAGILRDKMSFNME
                     EDEWDAVVNVHLKGHFCPSRHAGTYWRSQSKAGHEAGRRIINTSSEAGLFGNAGQANY
                     AAAKSGIATLAIVFGRELAAYGVTGNAIAPRARTAMTDSMEFFNAPDDESQFDRFLPE
                     NISPLVAWLASDDAADVNGQVFVVIGGDVWIMQGWSIAGHLHKDGPWTPEELAAKKDE
                     LFADHDRTLPGMAVPSW"
     gene            complement(2921..3955)
                     /locus_tag="VFP02_07660"
     CDS             complement(2921..3955)
                     /locus_tag="VFP02_07660"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HET9542942.1"
                     /translation="MTSTAPTTHQSLPSRWKEVLTTTNPPASGEMDVVSRWLFLTRAA
                     VLPMTLTAGLVAGLLAVHADDFNAGWFALAFLGILLAHTANNLMNDLFDSTTGLDKAD
                     YPRAMYSPHPVLEGYITKRGLATAAIVVNLVDAGVGVVLFMARGWPVIAFALAGFLLS
                     AAYTAPPLRLKKRGLGEPDVFVVWGPLMVGGVYYSAVGSIPWEVYAASVPYGLLCTTV
                     LMGKHVDKAPWDEREGIRTLPVILGDTRALAVTRGMMAGFYVTAVAGIAIGALPWPVV
                     VVALGLPTLVKAWGPLSEPKPAEPPKGFPIWPLWYAAIAFLHTRRAGALLVLGLLVAA
                     VAGIEPGFLS"
     gene            complement(3967..>4147)
                     /locus_tag="VFP02_07665"
     CDS             complement(3967..>4147)
                     /locus_tag="VFP02_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542943.1"
                     /translation="VPFHVGVDDIPEPGSTPPAGKRRGRAQVDGGGPDPVLDSAVGAA
                     AAEAVEAAERAAAEQ"
BASE COUNT          630 a         1385 c         1544 g          588 t
ORIGIN      
        1 aggtgggagt aggcccccgc cagcccgtcg acgtccaggc cgccgtcacc gatgacgaca
       61 tcggcgcccg cggccagctc gtcgacggtg ccgaccaccg cgcgcttgga ggtgttcagg
      121 aaccggaaga aggccgagtc ctcgtccgcc gggaggtcga cgccggcggc cgtccaccgg
      181 cgcaaggggt caccctcagg cccctcgacc ttgaccacgt ccgcgccggc gtcggccagg
      241 agcttggtgg cgtagggccc ggccacctcg gtcgaggcgt cgatgacccg cagtcccccc
      301 agcacgcctt cagcatggcc cacggcatca ctggtcgtga tcgggtggtt ctggttcccg
      361 gatccgggca ggtactcacg gatggttgcg ggtaggggag cgggcgggaa gctgagacaa
      421 cgaggtgaca gacatggccg aactttggac tttcgagatg ttggagatca ccgagcccgt
      481 cgacctgacg gggttcgacg tggaggcgtc cgatgggagc atcggcaaga tcgacgaagc
      541 gacctacgag gccggtgccg gctatgtggt cgtggacacc gggttctgga tcttcggcaa
      601 gaagcgcatg atcccggccg gcgccatcac cgacatggac ctcgacaacc ggacggtccg
      661 agtgaacctg accaaggacc agatcaaggc ggccccggac tatgaggcag cccagaggga
      721 cgacaccgac taccgggacc ggttgagcga ccactacggc gcctcgtgag ccgccggttc
      781 agccagccgc cagcgcggcg gtcagcccgt cgcggaagcg ggtgaggacc gaggtccagg
      841 cgtcccgcat gtcccgagcc tcgacgtcgt cgttcacgaa ctggtggacg tcgacccgac
      901 taccggtcgc ggtgggcagg acccgcaggt aggccgtgcg caggtcggcg gggaccggca
      961 cgtcatgggc gtcgaagtcc cagcgcatgg ccagcagcct gggcgggctc acgacgtcgt
     1021 agatgccgcg cacccggatg ccccgctcgg tttcacaggc gaagcgcccg tcggtgatcg
     1081 tcaccggcac gccgagccag cgcgagtaga cgtcgggctc gacgaacccg ctgaaggcgt
     1141 cagccggtgt ccggtcgacc tcgacgtgga gagcgacgac gtcggactcg accacggtca
     1201 ccccgtcgtc ggccggccgg tccgggggag cccaccgtcc ggtgtggctg agcagggcct
     1261 cgaagccccg gaaggcgtcc tggcgggccc ggtaccggcg gaagttcccc tccgcccgca
     1321 tggtgaccag gcctgcgccc cggaggacgg tgaggtgctg ggagatcgtc ggtgcggtca
     1381 cgtcgaacgc ggcggcgatg tcgccgaccg ccagctcctg gtcccagacc atccacagga
     1441 tctcgcggcg gaccggggag gacagcgcgt cgagcaaccg ctgaggccct tccgtcaccc
     1501 gcgcgacctt agcgggctgg acgaactatt tcgactatga tctaatttgg ttctcaccga
     1561 aacatggagg caggaaatgg cgaacttcgt actggcctat cggggtggca gcgtgccgga
     1621 cagtgaggcc gatcagcagg cggccatgga ggcgtggatg ggctggttcg gcagcctggg
     1681 cgacgccgtg gtcgacggtg gggcgccctt cggcgcctcc atgtcccgca ccggcgacgg
     1741 ggcaccggcc gacggcgccg actcggccct cacgggctac tccatcgtgt ccgccggctc
     1801 cctggccgag gcgaccgagc tggccggggg ctgccccgtc ctccagagcg gtggcagcgt
     1861 cgacgtctac gaggccatgc ccatgtgatc ccggttctgg tggccgcccg gtgtcgtggg
     1921 gacacggggg cagccaccag aacggggtgg tcaccagctg gggaccgcca tcccgggcag
     1981 ggtgcggtcg tggtcggcga agagctcgtc cttcttggcg gccagctcct cgggcgtcca
     2041 gggcccgtcc ttgtgcaggt gcccggcgat gctccagccc tgcatgatcc agacgtcgcc
     2101 cccgatgacc acgaagacct ggccgttgac gtcggcggcg tcgtccgacg ccagccaggc
     2161 gaccaggggc gagatgttct cgggcaggaa ccggtcgaac tggctctcgt cgtcgggggc
     2221 gttgaagaac tccatggagt cggtcatcgc cgtccgggcc cggggggcga tggcgttgcc
     2281 ggtcacgccg taggcggcca gctcccggcc gaagacgatg gccagcgtgg cgatgcccga
     2341 cttggccgcg gcgtagttgg cctggccggc gttgccgaac agcccggcct cactggaggt
     2401 gttgatgatc cggcggccgg cttcgtggcc cgccttggac tgcgagcgcc agtacgtgcc
     2461 ggcgtggcgg gacgggcaga agtggccctt gaggtggacg ttgacgaccg cgtcccactc
     2521 atcctcctcc atgttgaagg acatcttgtc ccgaaggatc ccggcgttgt tgaccaggat
     2581 gtggaggtcg ccgtaggtgt cgacggcctg ctcgatcatc tccttggcgg cgttccagtc
     2641 cgacacgctc gtgcggttgg ccacggcctc gccgcccgag gcggtgatct cggccacgac
     2701 gtcctcgacc ccgtcaccga ggtcgttgag cacgaggcgg gcgccctcgg cggcgaagag
     2761 cttggcgtgg gcggccccca ggccctgtcc ggcgccggtg atgatggcga ccttcccgtc
     2821 gagcgttccc atggctgcgt actcctgtgg ctggttggcg agcggccggt gagcttagga
     2881 cggcccctga cggggcgtca gatcccggga ccggacccgc tcagctcagg aagcccggtt
     2941 cgatgccggc cacggcggcg acgagcagcc cgaggacgag gagcgcgccc gcccgccggg
     3001 tgtgcaggaa ggcgatggcg gcgtaccaga gaggccagat cggaaatccc ttcggcggct
     3061 cggccggctt gggctccgac aacggccccc aggccttgac cagcgtcggc aggcccaagg
     3121 ccacgacgac gacgggccag ggcagcgcgc cgatggcgat gccggcgacg gcagtgacgt
     3181 agaagccggc catcatcccg cgggtgacgg ccagggcccg ggtgtcgccg aggatcacgg
     3241 gcagggtgcg gatgccctcc cgctcgtccc agggggcctt gtcgacgtgc ttgcccatga
     3301 ggacggtggt gcacagcagg ccgtagggga cggaggcggc gtagacctcc caggggatgg
     3361 agcccaccgc cgagtagtag acgccaccga ccatgagcgg gccccacacc acgaagacgt
     3421 cgggctcacc gagccctcgc ttcttcaggc ggagcggcgg ggccgtgtag gccgccgaga
     3481 ggaggaagcc ggccagggcg aaggcgatga cgggccagcc ccgggccatg aagagcacga
     3541 cgcccacgcc ggcgtccacg aggttcacca cgatggccgc cgtggccagg ccccgcttgg
     3601 tgatgtaccc ctcgagcacg gggtgaggcg agtacatggc ccgcgggtag tcggccttgt
     3661 ccaacccggt cgtgctgtcg aacaggtcgt tcatgaggtt gttggccgtg tgggccagga
     3721 ggatcccgag gaacgccagc gcgaaccagc cggcgttgaa gtcgtcggcg tggacggcga
     3781 gcaggccggc gaccaggccg gcggtcagcg tcatgggcag cacggccgcc cgggtcagga
     3841 agagccagcg gctgaccacg tccatctcgc cggaggcggg cgggttggtg gtggtcagca
     3901 cctccttcca gcgggacggg aggctctggt gtgtggtcgg agcggtcgac gtcacgaccg
     3961 cggaccctac tgctcggcgg cggctcgctc ggcggcctcg accgcctcgg cggcggccgc
     4021 gccgacggca ctgtcgagga cggggtccgg cccgccgccg tcaacctggg cacggccccg
     4081 cctcttgccg gcgggcgggg tggagccggg ctcagggatg tcgtccacgc ccacgtggaa
     4141 cggcacc
//
LOCUS       DASTFV010000415         2231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1539054, whole genome shotgun sequence.
ACCESSION   DASTFV010000415 DASTFV010000000
VERSION     DASTFV010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539054"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VFP02_07670"
     CDS             <1..851
                     /locus_tag="VFP02_07670"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HET9542944.1"
                     /translation="VPRVHARLDALPFRRSALAGAWAAKSYVHLGRPAVPLALADLHR
                     ALVVGAPADLTLFGGDVDAQAFADDPFAGRRFSGWPETMLRDVLVGGGFTVDDLEVEP
                     RADGIPYLLASVRRARTLPDTVGAGMRLLVCGLNPSIYSADVGTGFARPGNRYWPAAL
                     AAGLVSRDRDTRHALVAHAIGMTDLVKRATPRADALTTDEYRAGVERVERLVRWLQPG
                     AVVMVGLAGWRAAVDRKAVAGIQPEPFGNRPVYVMPSTSGLNASSRLDDLTDHLRQSA
                     ALADQA"
     gene            861..1847
                     /locus_tag="VFP02_07675"
     CDS             861..1847
                     /locus_tag="VFP02_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542945.1"
                     /translation="MPLRLEPVDEYLHELGPETTFNESMYFNVYDPAEQVGGFVRLGN
                     RANEGYAEMTTCLYLPDGRVGFMYHRPEISDNKAFTAGGMSFEVVEPFEELRVGYEGK
                     VVMLEDPLEMADPRKAFTENPYEQAKVELTYTRVSDPIGGEPTENHEREGEEFAKGHY
                     EQLNAATGTISVGDQEWAVSGYGLRDHSWGPRTWQAPWYYRWLTANFGDDFGFMGSRI
                     ARPDRDGMRGGFIWDGERILGCHHFEISTDWTETGTYHRAIEATLHHAEGALKVKGKV
                     LNLIPLRNRRDGRVTRISEGLTEWTLEDGRVGYGLSEYLDQIIDDQPVGLAE"
     gene            complement(1852..>2231)
                     /locus_tag="VFP02_07680"
     CDS             complement(1852..>2231)
                     /locus_tag="VFP02_07680"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HET9542946.1"
                     /translation="QEEARHLAFARMRLPELQQEASRWERWRMRHTVPFGIHRLFDSM
                     LDPGVYATVGLPPLRTWVKANRSERRLALRYEACRPILDAVVAAGFIEADQVPGPWRR
                     LCHVDRAGRPLPDSPALPAVPAA"
BASE COUNT          328 a          823 c          782 g          298 t
ORIGIN      
        1 cggtcccgcg ggtccacgcc cggctggacg ccctcccctt ccgccggagc gcgctggcgg
       61 gggcgtgggc agccaagtcc tatgtccacc tcggccggcc cgccgtcccg ctggcgctgg
      121 ccgacctcca ccgggcgctg gtcgtcgggg cgccggccga cctgaccctc ttcggcggtg
      181 acgtcgacgc gcaggccttc gccgacgacc ccttcgccgg acgtcgcttc tcgggctggc
      241 ccgagacgat gctgcgcgac gtactcgtcg ggggtgggtt cacggtcgac gacctggagg
      301 tcgagccccg cgccgacggc atcccctacc tgctcgcctc cgtgcgccgg gcgcggaccc
      361 tgcccgacac ggtcggcgcg ggcatgcgcc tgctggtgtg cgggctgaac ccgagcatct
      421 actccgcgga cgtggggacg ggcttcgccc gaccgggcaa ccgctactgg ccggccgccc
      481 tcgccgccgg cctggtcagc cgcgaccggg acacccgcca cgccctcgtc gcccacgcga
      541 tcggcatgac ggacctggtc aagcgggcca ccccccgggc cgacgccctc accaccgacg
      601 agtaccgcgc cggggtcgag cgagtcgagc ggctggtccg ctggctccag cccggcgcgg
      661 tggtcatggt cggcctcgcc ggttggcggg ccgccgtcga ccgcaaggcc gtggccggga
      721 tccagcccga gcccttcggc aaccggccgg tctacgtcat gcccagcacc agcggcctga
      781 acgcctccag ccgcctcgac gacctgaccg accacctccg tcagtccgcc gccctcgccg
      841 atcaggcgta ggttctggcc atgccgctgc gcctggagcc cgtcgacgag tacctgcacg
      901 agctgggccc ggagaccacg ttcaacgagt cgatgtactt caacgtgtac gacccggccg
      961 agcaggtcgg cgggttcgtg cgcctcggga accgggccaa cgagggttat gccgagatga
     1021 cgacctgcct ctatctgcct gacggccggg tcggcttcat gtaccaccgg cccgagatca
     1081 gcgacaacaa ggccttcact gcggggggca tgtccttcga agtggtcgag ccgttcgagg
     1141 agctgcgggt cggctacgag ggcaaggtcg tcatgctcga ggatcccctc gagatggccg
     1201 acccccgcaa ggcgttcacg gagaacccct acgagcaggc caaggtcgag ctgacctaca
     1261 cgcgggtcag cgaccccatc gggggtgagc ccaccgagaa ccacgagcgg gagggcgagg
     1321 agttcgccaa gggccactac gagcagctca acgccgccac cggcaccatc agcgtgggcg
     1381 atcaggaatg ggccgtctcc gggtacggcc tgcgggacca ctcctggggc ccccgcacgt
     1441 ggcaggcccc ctggtactac cgctggctga ccgccaactt cggcgacgac ttcgggttca
     1501 tgggctcccg catcgcccgc cccgaccgag acggcatgcg gggtgggttc atctgggacg
     1561 gcgagcgcat tctcggctgc caccacttcg agatcagcac cgactggacc gagaccggga
     1621 cgtaccaccg ggccatcgaa gccaccctgc accacgccga gggggctttg aaggtcaagg
     1681 gcaaggtgct gaacctgatc ccgctgcgca accggcgcga cggcagggtc acccgcatct
     1741 ccgagggcct caccgagtgg accctggagg acggccgggt gggctacggc ctgtcggagt
     1801 acctcgacca gatcatcgac gaccagcccg tcggcctcgc cgagtagccg gtcaggcggc
     1861 gggaaccgcg ggcagcgccg ggctgtccgg gagggggcgg ccggccctgt cgacatggca
     1921 gagccggcgc cacgggcccg gcacctggtc cgcctcgatg aacccggccg cgacgactgc
     1981 gtccaggatc gggcggcagg cttcatagcg cagggcgagt cgacgctcgg agcggttggc
     2041 cttcacccag gtccgcagcg ggggcaggcc caccgtcgca tagacccccg ggtcgagcat
     2101 ggagtcgaac aaccggtgga tgccgaaggg gacggtgtgg cgcatccgcc agcgctccca
     2161 ccggctggcc tcctgctgca gctcgggcag gcgcatgcgg gcgaaggcca ggtgtcgggc
     2221 ctcctcctgg c
//
LOCUS       DASTFV010000416         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1541389, whole genome shotgun sequence.
ACCESSION   DASTFV010000416 DASTFV010000000
VERSION     DASTFV010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541389"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VFP02_07685"
     CDS             <1..303
                     /locus_tag="VFP02_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542947.1"
                     /translation="EVVGDQVMAAVVPGPSGFDGAGLAAFLTEQADLGTKWAPRYVRV
                     MDALPATATNKYLKRELRAEGWAVSDPVWWRSERDGDYTELTDDDAAALRARFADR"
     gene            260..1123
                     /locus_tag="VFP02_07690"
     CDS             260..1123
                     /locus_tag="VFP02_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542948.1"
                     /translation="MTMLRPCGLGSPTAERLLTVPVDPLELAAALADALDTPLPSIGL
                     PAHLWLLGIGSHHDAAELVTHLFAGTGRSCRAIGADGHAQLSGLIGPADTVVLIGPDD
                     EFLEFGQRVAAGAGALVVELPEMGVEDDSTTRHLTAALRLARIALAGGAHGATAADLA
                     RIPGAVAAVAVDPQLPDIQPPKRALLVTGGGPAAVTARHAAVGFRAAGLPAEGVDAAD
                     LLGGLVTDLGAEDTLILLDPSRDVESPAASLSRLAGALGTTVHKMDGPLDLPPVCAQF
                     PLTVRLNLLAC"
     gene            1159..1884
                     /gene="merB"
                     /locus_tag="VFP02_07695"
     CDS             1159..1884
                     /gene="merB"
                     /locus_tag="VFP02_07695"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HET9542949.1"
                     /translation="MAQNVPTFEFTEDAKRVREFVFRFWSDERRPPNLRDVSQALDLD
                     RRQIIDAYKAMDLGIIFTVDLDTQNANLLKAPPFSAYPSQVAVFIDDEFHSYAGCASE
                     ATAMSNMPAFAGQELRLEGWCACCMEPITIRSKDFEITSTSHDGILMHISASPHDWNN
                     VSMVTMCDAMNFVLGPDHAERYERQISRRGVLLDLDQVKKFVTYTATQRAHDYHWPPQ
                     TMMPEIVIQLFEGYGVDCTNWRG"
     gene            1884..>2155
                     /locus_tag="VFP02_07700"
     CDS             1884..>2155
                     /locus_tag="VFP02_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET9542950.1"
                     /translation="MATEPTGDYIEVVVEIPKGSRNKYEIDHDSGEVWLDRNLFTATQ
                     YPADYGFIPNTLAGDGDPLDVLVLLDESTFPGCHIRARPIGVFWMK"
BASE COUNT          337 a          808 c          694 g          316 t
ORIGIN      
        1 gaggtcgtgg gcgatcaggt catggccgcg gtggtcccgg gcccgtcggg tttcgacggg
       61 gccggactgg ctgcttttct gaccgagcag gccgacctgg ggaccaagtg ggcacctcgc
      121 tacgtccggg tgatggacgc cctccccgcg acggcgacga acaagtacct gaagcgcgag
      181 ctgcgcgccg agggctgggc cgtttccgac ccggtctggt ggcgatccga gcgcgacggc
      241 gattacaccg aactgaccga tgacgatgct gcggccctgc gggctcggtt cgccgaccgc
      301 tgagcgcctg ctgaccgtgc ccgtcgaccc tctcgaactt gccgccgccc tggccgacgc
      361 gctggacacc ccgctcccct ccatcggcct gcccgctcac ctctggctcc ttggcatcgg
      421 tagccaccac gacgccgccg aactggtcac ccatctcttc gccggaaccg ggcggtcgtg
      481 ccgggccatc ggcgccgacg gccacgccca gctctccggc ctgatcgggc cggccgacac
      541 cgtggtcctc atcgggcccg atgacgagtt cctcgagttc ggccagcgcg tggccgccgg
      601 cgcgggcgcc ctggtcgtcg agctacccga gatgggggtc gaggacgaca gcaccacccg
      661 ccacctgacg gcggccctcc ggctggcccg catcgccctg gcggggggag cccacggcgc
      721 caccgcggct gacctggccc gcatcccggg ggcggtggcg gccgtggctg tcgaccctca
      781 gctcccggac atccagcccc ccaaacgagc cctcctggtg accggcggcg gcccggccgc
      841 ggtcaccgcc cgtcacgccg ccgtcggctt ccgggcggcg ggtctgcccg ccgagggcgt
      901 ggacgccgcc gacctgctcg gaggcctcgt caccgacctc ggcgcggagg acaccctgat
      961 cctgctcgac cccagccgcg acgtcgagag cccggccgcc tccctcagtc gcctggccgg
     1021 ggccctgggg acgacggtcc acaagatgga cggccccctc gacctgccac cggtgtgcgc
     1081 ccagttcccg ctgaccgtgc gcctgaacct gctcgcctgc tgacctaccg tcccaccgac
     1141 aaggagcact gatcatcgat ggcccagaac gtgccgacct tcgagttcac cgaggacgcc
     1201 aagcgcgtcc gggagttcgt gttccgcttc tggagtgacg agcggcgtcc gcccaacctg
     1261 cgcgacgtga gccaggccct cgacctggac cgccggcaga tcatcgacgc ctacaaggcg
     1321 atggacctcg ggatcatctt cacggtcgac ctcgacaccc agaacgccaa cctgctgaag
     1381 gccccaccgt tcagtgccta cccgagccag gtggcggtgt tcatcgacga cgagttccac
     1441 agctacgccg gctgcgccag cgaggcgacg gccatgtcca acatgccggc gttcgccggc
     1501 caggagctcc gcctcgaggg ctggtgcgcc tgctgcatgg agcccatcac catcaggtcg
     1561 aaggacttcg agatcacctc gacctcccac gacgggatcc tcatgcacat ctcggccagc
     1621 ccccacgact ggaacaacgt gagcatggtc accatgtgcg acgccatgaa cttcgtgctc
     1681 ggccccgacc acgccgagcg ctacgagcgt cagatctcgc gccggggcgt gctcctcgac
     1741 ctcgatcagg tcaagaagtt cgtcacctac accgccaccc agcgggccca tgactaccac
     1801 tggccaccgc agacgatgat gcccgagatc gtgatccagc tgttcgaggg ctacggcgtc
     1861 gactgcacca actggagggg ttgatggcca ccgaaccgac cggcgactac atcgaagtcg
     1921 tggtcgagat cccgaagggg tcaaggaaca agtacgagat cgaccacgac tcgggggagg
     1981 tctggctgga ccggaacctg ttcacggcga cgcagtaccc ggcggactac ggcttcatcc
     2041 cgaacacgct ggccggggac ggcgaccccc tggacgtgct ggtgctgctc gacgagtcga
     2101 ccttccccgg ctgtcacatc cgggcccgcc ccatcggcgt gttctggatg aagga
//
LOCUS       DASTFV010000417         2870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1542458, whole genome shotgun sequence.
ACCESSION   DASTFV010000417 DASTFV010000000
VERSION     DASTFV010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542458"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /gene="manB"
                     /locus_tag="VFP02_07705"
     CDS             complement(<1..1284)
                     /gene="manB"
                     /locus_tag="VFP02_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222900.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET9542951.1"
                     /translation="MASPSDRSAAPVAADRLAAVFKAYDIRGLAPEQLDADLCGAVGA
                     AFAAFVAEADGAERVLVARDMRPTGPALVDAFAAGVTSQGLDVVDVGLGSTDMLYFAA
                     GALDAPGAMFTASHNPAAYNGIKLCRSGARPVGEDTGLADIRRRAGAGVPPAATTGAR
                     HGDEVLSRFADHVRSFVDLDGLRPLKVVADTANGMGGLVVPAVFASLPFELEILYPEL
                     DGTFPNHPADPIQPENQRDLQARVLATGADVGLAFDGDADRVFLVDEKAQLLSGSLTT
                     AIVADEMLAREPGGTVIHNLICSKAVPEVVREAGGTPVRTRVGHSFIKAVMAETGAVF
                     GGEHSGHYYFRDNFRADSGLIAAIVVLDVLGRSGRPLSEARRPYERYAASGEINTEVP
                     DPRAVIDIVAGRFPDAEQDRLDGLTVDFGDWWFNLR"
     gene            complement(1290..2486)
                     /locus_tag="VFP02_07710"
     CDS             complement(1290..2486)
                     /locus_tag="VFP02_07710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9542952.1"
                     /translation="MRRLGPGAAVVLATGVDVGGLLVIRAVTGWPVAVADAIALLVAA
                     PVSFLLHRRADRGADPFVRWARDPRRFGRVAFVAGLTDLAVLQILVVAVGDPSLLTLF
                     GLKLVALTVAALVRVVGHRRILLRTVRADQAEPVARPAAPGDRRLSVILPSYHQPDVV
                     GEAVIRVRDVLVAALGADEVEVVVVDDGSGDDTATRAKAAGADQVVVHPENRGKGAAV
                     RSGAAVARGRTIAFTDADLAYDPAHLLVLLDRVEAGWDVAVGSRRHTDTVTLVRARRL
                     REFGGRFINVLTQAVLLGHYRDTQCGLKGFRSDVAPLVLGQGLIDGFAFDIELFHLVE
                     RNGLSLTEVPVQVANSDQSSVQVLRDGTRLVRDLFRIRRNAAAGRYRSDGAVTEPGAP
                     GPRSVG"
     gene            complement(2486..>2870)
                     /locus_tag="VFP02_07715"
     CDS             complement(2486..>2870)
                     /locus_tag="VFP02_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542953.1"
                     /translation="DEIGQPVLVRASYFPNWDTSGAEGPYRVAPNLMVVIPTENHVEL
                     HYGWTPVDLLAWLLTFLGIGGVILLARRPPVVVPVPPEPVATSVYGPDPPDAALGLEG
                     EEDEDEPEELVVEDEPDEVPVGAAD"
BASE COUNT          431 a          999 c         1097 g          343 t
ORIGIN      
        1 acggaggttg aaccaccagt ccccgaagtc cacggtcagg ccgtccagcc ggtcctgctc
       61 ggcgtcgggg aagcggccgg ccacgatgtc gatgacggct cggggatcgg gcacttcggt
      121 gttgatctca ccggacgcgg cgtagcgctc ataaggtcgg cgcgcctcgg agagcgggcg
      181 gccggagcgg ccgagcacgt cgaggaccac gatggcggcg atgaggccgg agtcggcccg
      241 gaagttgtcc cggaagtagt agtgccccga gtgttcgcca ccgaagacgg ccccggtctc
      301 ggccatcacg gccttgatga acgagtgacc gacccgggtg cgaaccggcg tcccgcctgc
      361 ctcccggacg acttcgggaa cggccttgga gcagatcagg ttgtggatca cggtgccgcc
      421 cggctcgcgg gccaacatct cgtcggcgac gatcgccgtg gtgagcgagc cggacaggag
      481 ctgagccttc tcgtcgacga ggaagacccg gtcggcgtcg ccgtcgaagg cgaggccgac
      541 gtcggcgccg gtcgccagga cgcgggcctg gaggtctcgc tggttctcgg gttggatcgg
      601 gtccgcgggg tgattgggga aggtgccgtc cagctccgga tagaggatct ccagctcgaa
      661 cggcagggac gcgaacacgg cgggaacgac gaggcccccc atcccgttgg cggtgtcggc
      721 caccaccttg agcggccgca agccgtcgag gtccacgaag gaccggacgt ggtcggcgaa
      781 acgggacagc acctcgtccc cgtggcgggc gccggttgtg gcggccggcg ggacaccggc
      841 tcccgcccgg cgtcggatgt cggccaggcc ggtgtcctcg ccgacgggac gggcgccgga
      901 gcgacagagc ttgatgccgt tgtaggccgc cgggttgtgg gaggcggtga acatcgcccc
      961 gggcgcgtcg agggcgccgg cggcgaagta gagcatgtcg gtcgaaccga ggcctacgtc
     1021 gacgacgtcg aggccctgac tggtcacccc cgcggcgaag gcgtcgacga gggcggggcc
     1081 ggtggggcgc atgtcccggg cgaccaggac ccgctcggcc ccgtcggcct ccgccacgaa
     1141 ggcggcgaag gcggcaccga cggctccaca caggtcggcg tcgagttgct cgggcgccag
     1201 gccgcggatg tcgtacgcct tgaacaccgc ggccaggcgg tccgcggcga cgggcgcggc
     1261 ggaacggtcg gatggggagg ccaagccggt caccctaccg aacgaggccc cggcgctccc
     1321 ggttcggtga cggcgccgtc actgcggtaa cggccggcgg cggcgtttcg ccggatccgg
     1381 aacaggtccc ggacgagccg ggtgccgtcc cggaggacct ggacgctgga ctggtcggag
     1441 ttggccacct ggaccgggac ctcggtgagg ctcaacccgt tgcgctcgac gaggtggaac
     1501 agctcgatgt cgaaggcgaa gccgtcgatc aggccctggc cgaggacgag gggggccacg
     1561 tcggagcgaa accccttgag cccgcactgg gtgtcccggt agtggccgag gaggacggcc
     1621 tgggtgagca cgttgatgaa ccggccaccg aactcccgca ggcggcgggc ccgtaccagg
     1681 gtgacggtgt cggtgtggcg acggctcccc acggcgacgt cccagcccgc ctcgacccgg
     1741 tcgagcagga ccagcaggtg ggccgggtcg taggcgagat cggcgtcggt gaaggcgatc
     1801 gttcgccccc gggccaccgc ggcgcccgaa cgcacggcgg cgcccttccc ccggttctcg
     1861 gggtggacca cgacctggtc ggcccccgcc gccttggccc gggtcgcggt gtcgtcgccg
     1921 gacccgtcgt cgaccaccac cacctcgacc tcgtcggctc ccagagcggc gacgagcacg
     1981 tcccgcaccc ggatgacggc ctcccccacc acgtcgggct ggtgatacga gggcaggatc
     2041 acgctcagcc gtcggtcacc gggcgccgcc gggcgggcca ccggctcggc ctggtcggcc
     2101 cgcaccgtgc gcaggaggat ccgacggtgg ccgaccaccc ggaccagcgc ggccacggtg
     2161 agcgcgacca gcttcaggcc gaacagggtc agcagggacg ggtcgcccac ggccacgacc
     2221 aggatctgca gcacggccag gtcggtcagc ccggccacga aggccacccg cccgaagcgg
     2281 cggggatcgc gggcccaacg gacgaacggg tcggcacccc ggtcggctcg gcgatgcagc
     2341 aggaacgaga ccggggccgc gaccagcagg gcgatggcgt cggccacggc cacgggccag
     2401 cccgtgacgg cacggatgac caacagccca ccgacgtcga cccccgtggc cagcacgacg
     2461 gccgcccccg ggccgagccg gcgcatcagt ccgccgcgcc caccgggacc tcgtcgggct
     2521 cgtcctcgac gacgagctcc tccggttcgt cctcgtcctc ctcgccctcg aggcccagcg
     2581 cggcgtcggg ggggtccggg ccgtagaccg acgtggcgac cggctcgggc gggacgggga
     2641 cgaccaccgg cgggcgccga gcgagcagga tgaccccgcc gatgcccagg aaggtgagca
     2701 gccaggccag caggtcgacc ggcgtccagc cgtagtgcaa ctcgacgtgg ttctcggtcg
     2761 ggatcacgac catgaggttg ggggcgaccc ggtacggccc ctcggccccg gacgtgtccc
     2821 agttcgggaa gtaggaggcc cggacgagca cgggttggcc gatctcgtcg
//
LOCUS       DASTFV010000418         6638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1544889, whole genome shotgun sequence.
ACCESSION   DASTFV010000418 DASTFV010000000
VERSION     DASTFV010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6638
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544889"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1927
                     /locus_tag="VFP02_07720"
     CDS             <1..1927
                     /locus_tag="VFP02_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542954.1"
                     /translation="QQIVGLIAVAAAAVTVTFTVSTPASSGTAATSPISPSRIADRGK
                     PVAAGSVQIDAAALADGDVTVATDPLPGLDDAFLTVSGTGEAATTAEIAVPTEAASTS
                     TAVIVDGDAPPDDLSAAALADASGSTIVATLSATDASGAVQSSSDKVWVDEFAGRTLV
                     SESGEQDLRLQRVAVLRNNRRVTAAEADALDAEVLGGGAARTESLGPGTCLDLCVSGT
                     VLWTDSAGSTHPVDRAPVEIRDANAGADTLVTTVTTDAGGHYAATIDNNDPDDATGRD
                     LYVRVRADGPGFTLNQFVESGVDTDIPTGSQVTQDLTTNNVDDNNTAFAVQASLVFAR
                     DYIVSLHGSQLPTVPVVFPDASGSFYDGTRLHILQPDRWDWDVSLHEYGHFVADRINI
                     EANPGGPHSFSANLSDVRHSKSIGTRLAWGEGWPTYFAVSALQETAAGLGIPNIGDTR
                     YQDTEDADIDVDLEAPHTLGEDNEATVMSVLWDLYDDVDEGSDHTSLGAQVVWDRLDD
                     NDPTTLSSAYRLFSPNRGNERVNCIFTRMNVSPRIDGPPNSSVSQVSPRISWARGNGG
                     THRNNRFSVVFRRSGGPILFTSPFRPGQTFRPTQAQWNAIRSQTLGALSVTVVGRQVD
                     APATGPYRSCTKSYIVH"
     gene            1980..2612
                     /locus_tag="VFP02_07725"
     CDS             1980..2612
                     /locus_tag="VFP02_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542955.1"
                     /translation="MPHPSEPGTLVLHGLRVKGFAETDVLADVTGLDDVTVVRALEAF
                     AAEGLVTRKTGRQVSGWVLTPQGRAEHEKRLADELDASGTRPAVETHYGEFTAVNPAL
                     LQVCTDWQLRDGAFNDHWDVAYDADVVARLVTLHEQARPVVTGLGAALDRFGGYGARL
                     QRAVDKVQAGDGDWFDKPMTDSYHTVWFELHEDLLATLGLERAAETGGEG"
     gene            2612..3496
                     /gene="dapA"
                     /locus_tag="VFP02_07730"
     CDS             2612..3496
                     /gene="dapA"
                     /locus_tag="VFP02_07730"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET9542956.1"
                     /translation="MGRFGSVLVAMVTPFDDDLELDLEAAVTLARWLVDQGADGLVLA
                     GTTGEAPTLTDDESVDLWAAVRGAVTVPLLAGTGSNDTRHTCQLSRRAAAAGVDGLLV
                     VSPYYNRPSQAGLEAHFRAVADATDLPFLIYDIPIRTGRKVDSEVLVRLAREVPTMVG
                     IKDAAGDPAASARVIAEAPDGFDLYSGDDAFTLPLLAIGGTGVIGVAAHWSAPEHAEM
                     ISAHAKGDVETARLVNARLLDSYDFETWGGAPQAVTTKALLRTLGLRVGLTRPPIGPV
                     PEGLEDAARGVLDGLGPR"
     gene            3496..5160
                     /locus_tag="VFP02_07735"
     CDS             3496..5160
                     /locus_tag="VFP02_07735"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET9542957.1"
                     /translation="MAPPVHITFLGGLGEIGRNCACIESDGKIVLIDCGIMFPDIDMP
                     GIDLVLPDFTYLRANADRVVGCIATHGHEDHVGGLSFLLRELKFPIYGSELTLGLARH
                     RIEEAGLVDRTDFRIVADNERLKIGPFDCEFLPVTHSVPHGFATAIHTPQGTILHTGD
                     FKLDLNPVDGRLTDLSVMGAIAQNEGIRLLLSDSTNADSPGHSRSETSVGAVLYNVMH
                     AHEGRRLIVTCFASHIHRVQQVADAAQSFGRRVATLGLSMKKNVALARKLGVLRIPDS
                     VLIDIDDIDGLPPGEVCVISTGSQGEPMSALARLASGDNRWLEIQENDTVILSSHPIP
                     GNETGVTKVIDGLTRLGAKVVHTGLADVHATGHAKQEELKTILSVTKPEWMIPVHGEY
                     RHLTAHADLARQMGVDPDHVLLCEDGDRIELGDKGITRVESVPAGYLYVDGTVGDVGD
                     GVLRDRKVLAEEGVVVVIVTVDMKSGAIITGPEIVTRGWVYAPEAEDLLDEAADAVRK
                     SIRDAVKKDKVIELENLQRHVRKAAGKFVSDKTKRRPMIVPVVMEA"
     gene            5216..6214
                     /gene="bioB"
                     /locus_tag="VFP02_07740"
     CDS             5216..6214
                     /gene="bioB"
                     /locus_tag="VFP02_07740"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HET9542958.1"
                     /translation="MIADPLGMARTILLDERRTVCMDILGALAQLSDEDVPALAALAH
                     EVRLAWNGPTVEIEGILSAKTGGCPEDCNFCSQSAQFDTPVQATPFLDTGEVLQAARE
                     TKALGATEFCIVLAVRGPDERTMARLEELVPQVQEEVGIDVAVSAGILTSEQAQRLAS
                     VGVHRYNHNLETAKSFFGQIVTTHSWDERFATCRLVRAHDMELCCGVLLGMGESDEQR
                     LELIAELRAVEPCEVPMNFLNPRPGTPLGDRPLVGALEAIRWIALFRIALPDVILRYA
                     GGREVTLRELQVMGLTSGINALIVGNYLTTLGRSPEEDLQMLEDLRMPIGALSKVI"
     gene            complement(6223..6405)
                     /locus_tag="VFP02_07745"
     CDS             complement(6223..6405)
                     /locus_tag="VFP02_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542959.1"
                     /translation="MSEMSFCTGCGRPVDSCEGGCTRTLDPPRFCPVCGRRSRVDVTP
                     TGYESRCRDHGTFKKG"
     gene            complement(6402..>6638)
                     /locus_tag="VFP02_07750"
     CDS             complement(6402..>6638)
                     /locus_tag="VFP02_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542960.1"
                     /translation="GRDDVGFGWFSRADPGPAEGLLVAMEGGPGYATTASRDYYAELY
                     APVREHRDLLVMDLRGTGTVTVDGRDVEAQARYA"
BASE COUNT         1014 a         2454 c         2236 g          934 t
ORIGIN      
        1 ccagcagatc gtcgggttga tcgccgtcgc ggccgcggcc gtgaccgtga ccttcaccgt
       61 gtccaccccg gcgtcgtcgg gaacggccgc gacctcgccg atctcaccct cgcggatcgc
      121 cgatcggggc aagccggtcg cggccggcag cgtccagatc gatgcggccg ccctcgccga
      181 cggcgacgtg acggtggcca ccgacccgct gccgggactc gacgacgcct tcctgacggt
      241 ctccggcacc ggggaggccg ccaccacggc cgagatcgcc gtgcccacgg aggcggcgtc
      301 cacgtccacc gcggtcatcg tggatggcga cgccccgccg gacgacctct cggcggctgc
      361 cctggctgat gcgtcgggct ccacgatcgt ggccacgctc agcgccactg acgcctccgg
      421 cgccgtccag tcgtcctccg acaaggtctg ggtcgacgag ttcgcgggcc ggaccctggt
      481 cagcgagagc ggggagcagg acctgcgcct ccagcgggtc gccgtcctcc ggaacaaccg
      541 cagggtgacc gcggcggagg ccgatgccct ggacgccgag gtcctcggcg gtggcgcggc
      601 ccggaccgag tccctcgggc ccgggacctg cctcgacctc tgcgtcagcg ggacggtcct
      661 gtggaccgat tctgccggca gcacgcaccc ggtcgaccgg gccccggtgg agatccggga
      721 cgccaatgcc ggtgccgaca cgctcgtcac gacggtcacg accgacgccg gcgggcacta
      781 cgcggcgacg atcgacaaca acgaccccga tgacgccacg ggccgggatc tctacgtccg
      841 ggtgcgggcc gacggtccgg ggttcacgct caaccagttc gtcgagagcg gcgtggacac
      901 ggacatcccc acgggttcgc aggtcaccca ggacctcacg accaacaacg tcgacgacaa
      961 caacaccgcc ttcgccgtcc aggcctcgct cgtcttcgcc cgggactaca tcgtcagcct
     1021 gcacgggtcg cagctcccca cggtgccggt ggtgttcccg gacgcgtccg gctcgttcta
     1081 cgacgggacg cgcctccaca tcctccagcc cgaccggtgg gactgggacg tgagcctcca
     1141 cgagtacggg cacttcgtcg ccgaccggat caacatcgag gccaaccccg gcggccctca
     1201 cagcttcagc gccaacctct ccgatgtccg ccacagcaag tcgatcggga cccgactggc
     1261 ctggggcgag gggtggccca cctacttcgc cgtgtccgcc ctgcaggaga ccgccgcggg
     1321 cctggggatc cccaacatcg gcgacacccg ctatcaggac accgaggacg ccgacatcga
     1381 cgtcgacctc gaggcgccgc acacgttggg cgaggacaac gaggccacgg tgatgagcgt
     1441 cctctgggat ctctacgacg acgtggatga gggcagtgac cacacgagcc tgggcgcgca
     1501 ggtggtctgg gaccggctcg acgacaacga cccgacgacc ctgtcctccg cctaccgact
     1561 gttcagcccg aaccggggca acgagcgcgt caactgcatc ttcacccgga tgaacgtcag
     1621 cccccggatc gacggtccgc ccaacagcag cgtgagccag gtgtccccgc ggatcagctg
     1681 ggctcggggc aacggcggga cgcaccggaa caaccgcttc tcggtggtgt tccgccgctc
     1741 cggggggccg atcctcttca cgtcgccgtt ccggccgggt cagaccttcc ggccgaccca
     1801 ggcccagtgg aacgccatcc gcagccagac gctgggcgcc ctgtcggtga cggtcgtcgg
     1861 tcggcaggtc gacgccccgg ccaccggtcc ctaccggagc tgcaccaaga gctacatcgt
     1921 ccactgagcc gatccctttc gtaccggggc ggtcccggcg gccccggtac gatcgctcca
     1981 tgccccaccc gtccgagccc ggcaccctcg tgctgcacgg gctccgcgtg aaggggttcg
     2041 ccgagaccga cgtgctggcc gacgtcaccg ggttggacga cgtgaccgtc gtgcgggcgc
     2101 tggaggcctt cgccgccgag ggcctcgtca cccgcaagac cggccggcag gtgtcggggt
     2161 gggtgctcac cccgcagggt cgggccgagc acgagaagcg actggccgac gagctcgacg
     2221 ccagcggcac ccgccccgcg gtggagacgc actacggcga gttcaccgcc gtcaaccccg
     2281 ccctcctcca ggtctgcacc gactggcagc tccgggacgg ggccttcaac gaccactggg
     2341 atgtcgcgta cgacgccgac gtggtcgccc gcctggtcac cctgcacgaa caggcccggc
     2401 ccgtcgtgac cggcctcggc gccgcgctgg accgcttcgg gggctacggc gcccggctgc
     2461 agcgggcggt ggacaaggtc caggccggcg acggcgactg gttcgacaag ccgatgaccg
     2521 actcgtacca caccgtgtgg ttcgagctcc acgaggacct gttggccacg ctcggcctcg
     2581 agcgggcggc cgagaccgga ggagagggct gatggggcgc ttcggttcgg tgctggtggc
     2641 catggtcacc cccttcgacg acgacctcga gttggacctg gaggccgcgg tcacgctggc
     2701 tcggtggttg gtcgaccagg gggccgacgg cctggtcctg gccggcacca ccggtgaggc
     2761 gccgacgctc accgacgacg agtccgtcga cctctgggct gctgtgcgcg gggccgtcac
     2821 cgtccccctc ctggcgggga ccggcagcaa cgacacccgt cacacctgcc agctcagccg
     2881 gcgggccgcg gccgccggtg tcgacgggct cctcgtcgtc tcgccgtact acaaccgacc
     2941 gagccaggcg gggctggagg ctcacttccg ggccgtggcc gacgccaccg acctgccgtt
     3001 cctgatctac gacatcccga tccgcaccgg ccgcaaggtc gacagcgagg tgctggtgcg
     3061 cctggcccgc gaggtaccga cgatggtcgg catcaaggac gcggcaggcg atccggcggc
     3121 gtcggcccgg gtcatcgccg aggcccccga cggcttcgat ctctacagcg gcgacgacgc
     3181 cttcacgctg cccctcctcg ccatcggggg caccggggtg atcggcgtgg ccgcgcactg
     3241 gtcggcgccc gagcacgcgg agatgatctc ggcccacgcc aaaggcgatg tcgagaccgc
     3301 tcggctcgtg aacgcccgac tgctcgactc ctacgacttc gaaacctggg gtggagcccc
     3361 acaagctgtg accaccaaag ccctgctgcg caccctcggc ctccgggtcg ggctcacccg
     3421 tccaccgatc gggcccgttc ccgaaggcct cgaggacgcc gcccgcggcg tgctggacgg
     3481 gctcgggccc cgctagtggc gcctccggtc cacatcacct tcctgggcgg cctgggcgag
     3541 atcgggcgca actgtgcgtg catcgagtcg gacggcaaga tcgtcctcat cgactgcggg
     3601 atcatgttcc cggacatcga catgcccggc atcgacctcg tcctgcccga cttcacctac
     3661 ctgcgggcga acgccgaccg ggtggtcggc tgcatcgcca cccacggcca cgaggaccac
     3721 gtcggcggcc tgtcgttcct gctccgggag ctgaagttcc cgatctatgg ctccgagctg
     3781 accctcggcc tggcccggca ccgcatcgag gaggcgggcc tggtcgaccg caccgacttc
     3841 cggatcgtgg ccgacaacga gcggctgaag atcggcccgt tcgactgcga gttcctcccc
     3901 gtcacccact cggtgcccca cggcttcgcc accgccatcc acaccccgca gggcacgatc
     3961 ctgcacaccg gcgacttcaa gctggacctg aacccggtgg acggccgcct caccgacctc
     4021 agcgtgatgg gcgccatcgc ccagaacgag ggcatccgcc tcctcctgtc ggactcgacc
     4081 aacgccgact cgcccggcca ctcccggtcg gagacctcgg tcggcgccgt cctgtacaac
     4141 gtcatgcacg cccacgaggg ccgccgcctg atcgtcacct gcttcgcctc gcacatccac
     4201 cgggtccagc aggtcgccga tgccgcccag tccttcggcc gtcgggtggc caccctcggc
     4261 ctgtccatga agaagaacgt ggccctggct cgcaagctgg gcgtgttgcg catccccgac
     4321 tcggtgctca tcgacatcga cgacatcgac ggcctgccgc ccggcgaggt gtgcgtgatc
     4381 tccaccggct cgcagggtga gccgatgtcg gccctggccc gactggcctc gggtgacaac
     4441 cgctggctgg agatccagga gaacgacacg gtcatcctca gctcgcaccc gatccccggc
     4501 aacgagaccg gcgtgaccaa ggtcatcgac ggcctgacgc ggttgggcgc caaggtcgtg
     4561 cacaccgggc tggccgacgt gcacgccacc ggccacgcca agcaggagga gctgaagacc
     4621 atcctgtcgg tgaccaagcc cgagtggatg atcccggtcc acggtgagta ccggcacctg
     4681 accgcccacg ccgacctggc ccgccagatg ggcgtcgatc ccgaccacgt cctgctgtgt
     4741 gaggacggcg accgcatcga gctgggcgac aagggcatca cgcgggtcga gagcgtccct
     4801 gccggctacc tctatgtcga cggcacggtc ggcgacgtcg gtgacggggt ccttcgggac
     4861 cgaaaggtcc tggccgagga gggggtggtc gtcgtcatcg tcaccgtcga catgaagtcc
     4921 ggcgccatca tcaccgggcc ggagatcgtc acccggggct gggtctacgc ccccgaggcc
     4981 gaggacctgc tcgacgaggc ggccgatgcg gtgcgcaaga gcatccgcga cgcggtgaag
     5041 aaggacaagg tcatcgagct cgagaacctc caacgtcacg tgcgcaaggc ggccgggaag
     5101 ttcgtcagcg acaagaccaa gcgccgaccc atgatcgtcc ccgtggtcat ggaggcctga
     5161 tctcccggtc tcgcggacta cgaccgacgg gaaccaagcg tctacgtttc gcgccgtgat
     5221 cgctgacccg ctgggcatgg cccggacgat cctgctcgac gaacggcgca ccgtctgcat
     5281 ggacatcctg ggcgcgctgg cccagctgtc cgacgaggac gtccccgcgc tggcggcgct
     5341 ggcccacgag gtccggctgg cctggaacgg gccgaccgtc gagatcgaag ggatcctgtc
     5401 ggccaagacg ggcggctgcc ccgaggactg caacttctgc tcgcagtcgg cccagttcga
     5461 cacccccgtt caggccaccc cgttcctgga caccggcgag gtgctgcagg ccgcccggga
     5521 gaccaaggcc ctgggggcca cggagttctg catcgtgctg gccgtgcggg gccccgacga
     5581 gcggacgatg gcccgcctcg aagagctggt cccccaggtg caggaggagg tggggatcga
     5641 cgtggccgtc agcgccggga tcctgacctc cgagcaggcc cagcgcctgg cctcggtcgg
     5701 cgtccaccgc tacaaccaca acctggagac ggccaagagc ttcttcggcc agatcgtgac
     5761 gacccacagc tgggacgagc gcttcgccac ctgccgcctc gtgcgcgccc acgacatgga
     5821 gctctgctgc ggcgtcctgc tgggcatggg cgagagcgac gagcagcgcc tcgagctcat
     5881 cgccgagctc cgggcggtgg agccctgcga ggtccccatg aacttcctca acccccggcc
     5941 ggggacgccg ctcggcgacc gtccactggt gggggcgttg gaggccatcc gctggatcgc
     6001 cctgttccgc atcgccctgc ccgacgtgat cctgcgctat gccgggggtc gcgaggtcac
     6061 gctgcgggag ctgcaggtca tgggcctcac gtcaggcatc aacgccctga tcgtgggcaa
     6121 ctatctgacc acgctcggcc gttcgcccga ggaggacctc cagatgctgg aggacctccg
     6181 catgccgatc ggcgccctct ccaaggtgat ctgaggaggc gttcagccct tcttgaacgt
     6241 cccgtggtcc cggcaccggg actcgtagcc cgtgggggtc acgtcgaccc gcgaacgccg
     6301 gccgcagacc gggcagaagc ggggcgggtc gagcgtcctc gtgcagccgc cctcacacga
     6361 atcgaccggg cggccacagc ccgtgcagaa cgacatctcg ctcatgcgta gcgggcttgg
     6421 gcttcgacgt cgcggccgtc gacggtgacg gtcccggtac cccggagatc catgacgagg
     6481 aggtcccggt gctcccggac cggggcgtag agctcggcgt agtagtcccg gctggcggtg
     6541 gtggcgtaac ccgggccgcc ctccatggcc accagcagcc cctctgccgg tccggggtcg
     6601 gcgcgcgaga accacccgaa gccgacgtcg tcgcggcc
//
LOCUS       DASTFV010000419         2932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1548776, whole genome shotgun sequence.
ACCESSION   DASTFV010000419 DASTFV010000000
VERSION     DASTFV010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548776"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFP02_07755"
     CDS             <1..294
                     /locus_tag="VFP02_07755"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9542961.1"
                     /translation="AADAFAGFSAGFEQLMAGDPAGAVTTVDDALRTLPGEDNLRFLR
                     GGALMASGNVDAGVAELRTLIADRPSWAVIVRSFGEGGLLPLPDGVTIDDVLG"
     gene            complement(295..2457)
                     /locus_tag="VFP02_07760"
     CDS             complement(295..2457)
                     /locus_tag="VFP02_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET9542962.1"
                     /translation="MTDTVAPVGTSAPPRIGTSVPRPDGVPKVRGRFAFGSDLWADEA
                     LFGRTVRSPHAAARIRSVDIGPALIVPGVYAVLVADDVPGESHYGLDRADQPVFASDV
                     IRYQGEAVAAVAADHPETARRAAAAVEVDYDLLEPVVDAEAAVTAEPLHPFGNVFREL
                     VIRHGDSNAHGEVVVEGTYEVGMQDQAFMGPESGMAIPTEDGGIDLFVSTQWLHSDRD
                     QVAACLGLPLEKVRLTLAGVGGAFGAREDLSLHVHASLLALHTGRPVRMSYTRVESFV
                     GHVHRHPARMHYRHEADGDGRLVRVDARLLFDGGAYQSSSAAVLGNASCFAAGPYLVP
                     NAVIDGKVVRTNNPPCGAMRGFGAVQTGFAVEAQMDKLAAALDLDPVELRLRNALKPG
                     DELLTGQVITGTAPVAEVIRACADHPLPPPAGEDVLDRPGGAGLTADPGDVRRGVGFA
                     VGFKNLMFSEGFDDYSTASCRLENGVVTVKSACAEVGQGFVTLVRQIVHEVLGVDDVV
                     LAPVDTDIGSAGSTSASRQTWMSGGAVQAACEAVKRRIDEGATGFIEEEVTFRHAATA
                     PLDGDGQGDAHVSFAFAAHRAVVDVDPELGLVRVVDMTTAQDVGRVLNPLQVVGQIEG
                     GTLQGVGLAVMEEMKLSGGQVLNPTFTDYLIPTALDAPEVRIAALIEEPEPGAPFGAK
                     GVGEPPTLSSTPAVAAAIRAATGRELPRVPIRPEDICL"
     gene            complement(2450..>2932)
                     /locus_tag="VFP02_07765"
     CDS             complement(2450..>2932)
                     /locus_tag="VFP02_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9542963.1"
                     /translation="VNLRYALLVNSAVHEVEDAWLGESLLNVLRDRLDLPGTKNACEE
                     GECGSCSVRVDGALVCSCLVLAASAVGSAVETVEAQEHDGALSAVQAAFVEEGAVQCG
                     FCTPGLVMAVDDLLDRSPTPTDLEIREAISGNLCRCTGYGRVIEAIRVAARQRSATGD
                     "
BASE COUNT          411 a         1022 c         1102 g          397 t
ORIGIN      
        1 gccgccgacg ccttcgccgg gttctccgca ggcttcgagc agctcatggc gggcgacccg
       61 gctggggcgg tgacgaccgt cgatgatgcg ctcaggaccc tgccgggtga ggataacctc
      121 cgcttcctgc ggggtggggc gctcatggcg tccggcaacg tggacgcggg ggtcgccgag
      181 ctccggacgt tgatcgccga tcggccgtcc tgggccgtga tcgtccgcag cttcggcgag
      241 ggcgggctcc tcccgctgcc ggacggcgtg acgatcgacg acgtcctcgg ctgatcagag
      301 gcagatgtcc tcggggcgga tggggacgcg ggggagctct cggccggtcg cagcccggat
      361 ggcggcggcc accgccgggg tcgacgacag cgtcggcggt tcgcccacgc ccttcgcccc
      421 gaagggggcg cccggctcag gctcctcgat gagggcggcg atgcggacct cgggggcgtc
      481 cagcgcggtg gggatgaggt agtcggtgaa ggtcgggttg aggacctggc cccccgacag
      541 cttcatctcc tccatgacgg ccagcccgac gccctgcagg gtgcccccct cgatctggcc
      601 cacgacctgc agggggttga ggacacggcc gacgtcctgt gccgtggtca tgtcgacgac
      661 ccggaccagg cccagctcgg ggtcgacgtc gacgacggcg cggtgggcgg cgaaggcgaa
      721 cgagacgtgg gcatcgccct gtccgtcccc gtccaggggg gcggtggcgg cgtgacggaa
      781 cgtgacctcc tcctcgatga acccggtggc cccctcgtcg atccgccgct tgaccgcctc
      841 gcaggcggcc tgcaccgcac cacccgacat ccacgtctgg cggctggccg aggtcgagcc
      901 cgcggagccg atgtcggtgt cgaccggcgc cagcacgaca tcgtcgactc cgaggacctc
      961 gtggacgatc tggcgcacga gggtgacgaa gccctgcccg acctcggcgc aggcgctctt
     1021 gacggtcacc accccgttct ccagccggca ggacgcggtc gagtagtcgt cgaagccctc
     1081 ggagaacatg aggttcttga acccgacggc gaagccgacc ccccggcgca cgtcaccggg
     1141 gtcggcggtg aggccggcgc cgccgggacg atcgagcacg tcctctcccg caggcggggg
     1201 aagcgggtgg tcggcacacg cccggatcac ctcggcgacc ggagcggtcc cggtgatcac
     1261 ctggccggtg agcagctcgt cgcccggctt caacgcgttg cgcaagcgga gctcgacggg
     1321 gtcgaggtcg agggcggcgg ccagcttgtc catctgggct tcgaccgcga aaccggtctg
     1381 cacggccccg aagccccgca tcgccccgca gggcgggttg ttggtccgca cgaccttgcc
     1441 gtcgatcacg gcgttgggca cgagataggg gccggcggcg aagcaggagg cgttgcccag
     1501 cacggccgcc gacgacgact ggtaagcgcc gccgtcgaag agcaggcggg catcgacccg
     1561 cacgaggcgg ccgtccccgt cggcctcgtg gcggtagtgc atccgagccg ggtggcggtg
     1621 gacatggccg acgaaggact cgacccgggt gtagctcatc cgcacgggcc gcccggtgtg
     1681 gagggcgagg aggctggcat ggacgtggag gctgaggtcc tcgcgggcgc cgaacgcccc
     1741 cccgacgccg gccagggtga gccgaacctt ctccagtggc agcccgaggc aggccgccac
     1801 ctggtcccgg tccgagtgca gccactgggt cgacacgaac aggtcgatcc caccgtcctc
     1861 ggtggggatg gccatgccgg actcggggcc catgaacgcc tggtcctgca tcccgacctc
     1921 gtaggtcccc tcgaccacca cctcgccgtg ggcgttgctg tcgccgtggc ggatcaccaa
     1981 ctcgcggaac acgttgccga aggggtggag gggctcggcg gtgaccgccg cttcggcgtc
     2041 gaccacgggc tcgagcaggt cgtagtcgac ctcgacggcc gccgcggccc ggcgggcggt
     2101 ctccggatgg tcggcggcca cggcggcgac agcctcgcct tggtaccgga tgacgtcgga
     2161 ggcgaacacc ggctgatcgg cccggtcgag gccgtagtgg ctctcgccgg gcacgtcgtc
     2221 ggcgaccagc acggcgtaca cgcccggcac gatgagggcg gggccgatgt cgaccgagcg
     2281 gatccgggcg gcggcgtggg gggagcgcac ggtacgtccg aagagggctt cgtcggccca
     2341 gaggtccgag ccgaaggcga accgtccccg caccttcggg acaccgtcag gacgggggac
     2401 gctggtcccg atgcgcggcg gggcgctggt cccgacgggt gcgaccgtgt cagtcaccgg
     2461 tcgcgctccg ctgacgggcg gccacccgaa tcgcctcgat gacccggccg tagccggtgc
     2521 accggcacag gttccccgag atggcttcgc ggatctcgag gtcggtgggg gtcgggctgc
     2581 gatcgaggag gtcgtcgacc gccatcacca ggccgggtgt gcagaacccg cactggacgg
     2641 ccccttcttc gacgaaggcg gcctgcacgg ccgagagggc gccatcgtgc tcctgggctt
     2701 cgaccgtctc caccgcgctc ccgacggcgg acgcggccag gaccaggcac gagcagacga
     2761 gggcgccgtc gaccctgacc gagcacgagc cgcactcgcc ctcctcacag gcgttcttgg
     2821 tgccgggcag gtcgagccgg tcccgcagga cgttgagcag gctctcgccc agccacgcgt
     2881 cctcgacctc gtggacggcg ctgttgacca gcagggcgta gcggaggttc ac
//
LOCUS       DASTFV010000420         3153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1553883, whole genome shotgun sequence.
ACCESSION   DASTFV010000420 DASTFV010000000
VERSION     DASTFV010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553883"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFP02_07770"
     CDS             <1..307
                     /locus_tag="VFP02_07770"
                     /inference="COORDINATES: protein motif:HMM:NF039286.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542964.1"
                     /translation="FTGIIDTVFMSGSTPIDWEWTLSVKAYKVRKLGTDGEALAKTAR
                     VGDALIADLRKQMAEDNVIFDNKVHNPSPALIDADGPILPFRRWAARFYPEPTRSGA"
     gene            complement(352..1368)
                     /locus_tag="VFP02_07775"
     CDS             complement(352..1368)
                     /locus_tag="VFP02_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET9542965.1"
                     /translation="MSGLKLGYQYGYWTAEPRPATDLIAAAQRAEALGYDSLWTGESW
                     SSDAFAPLAAVAGATSSIRLCTGIAQIGARPPTTAAMHAITLDGLSGGRAVLGLGVGG
                     PQVAEGWYGRPFAKPLARTREYVDIVRRTVAREAPVTSDGPHYPLPYAGDGAVGLGKP
                     LKLITHPLRPRIPILLGAEGPKNVALAFEIADGWLPLYFPPSRADLYDLPAVPDGFEI
                     AVNVSVFVDDDVATALWPVKATLGFYIGGMGAKGRNFHTELMARMGFEQEAFEIQDLF
                     FAGRRDEAIAAVPDDFADEISLCGPKKRIAERLEAWKAGPVSTLIVGSSDPVVLELMA
                     ELTA"
     gene            complement(1365..1727)
                     /locus_tag="VFP02_07780"
     CDS             complement(1365..1727)
                     /locus_tag="VFP02_07780"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9542966.1"
                     /translation="MTTPNVRAAELSTEDLEDWGTLEPPLATALGEPMATYGLDLWAA
                     DDESVSSGIWECEPGNSRWDLEEGEFIHVLSGRMTCRHDDGTEVELSAGSTAVFPKGW
                     AGTWEIHETLRKVYVLFP"
     gene            1795..2988
                     /locus_tag="VFP02_07785"
     CDS             1795..2988
                     /locus_tag="VFP02_07785"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9542967.1"
                     /translation="MPSPAFDRDDLLAKAQERAGGLTDLGPGPFLDGMDRFVDSLNAE
                     ARLNDIGRFIAEERVLLHVTNRLLYTHDREQHPEIASVDVVKPVFIIGLPRTGTTILH
                     DILNQDPANRAPLTWELMFPSPPPEASIGDEDPRVAACQATIPDDDLQSTLFKAMHPM
                     GATLSQECVVMMGEAMVTPLFHNQFRVPAYQDWVDDGADWSAVYAFHRRQLQHLWWHK
                     PGDRWMLKTGAHMWGLDQLLATYPDARIVFTHRDPVKSMTSYASLTTLVRSMGSDDVD
                     PHEIAADWTARLKRMLERSMAVRTARDYPDAVFYDMYFSDFVADQFAEVERIYDALDL
                     PMSAEGATGMRQYIADHPKGVDGIHRYRPEEYGVDPDTVRRDFAPYIERFGLEPEAPE
                     PEDGP"
BASE COUNT          491 a         1171 c         1053 g          438 t
ORIGIN      
        1 cttcaccggg atcatcgaca ccgtcttcat gagcggctcg accccgatcg actgggagtg
       61 gacgctgtcg gtcaaggcct acaaggtgcg caagctgggc accgacggcg aggccctggc
      121 caagaccgcc cgggtgggcg acgccctcat cgccgacctg cgcaagcaga tggccgagga
      181 caacgtcatc ttcgacaaca aggtccacaa cccgagcccc gccctcatcg acgccgacgg
      241 ccccatcctc cccttccgcc gctgggccgc ccgcttctac cccgaaccca cccgttctgg
      301 cgcctgattg cggcgtcgtg gcgactcatc gggcaccaga acgggggcag gtcaggcggt
      361 gagctcggcc atgagctcga ggacgacagg gtcgctgctg ccgacgatca gggtcgacac
      421 ggggccggcc ttccaggcct cgaggcgctc cgcgatgcgt ttcttcgggc cgcagaggct
      481 gatctcgtcg gcaaagtcgt cggggacggc ggcgatggcc tcgtcccgcc ggcccgcgaa
      541 gaagaggtcc tgtatctcga aggcctcctg ctcgaagccc atgcgggcca tcagctcggt
      601 gtggaagtta cggcccttgg cccccatccc cccgatgtag aagccgaggg tcgccttgac
      661 cggccacaac gcggtggcca cgtcgtcgtc cacgaacacc gacacgttca ccgcgatctc
      721 gaagccgtcg ggcaccgccg gcaggtcgta gaggtccgcc cgcgacggcg ggaagtagag
      781 cgggagccag ccgtcggcga tctcgaaggc gagggcgacg ttcttgggtc cctcggcacc
      841 gaggaggatc gggatgcggg ggcgcaacgg gtgggtgatc agcttcagcg gcttgcccag
      901 gccgaccgcg ccgtcgccgg catagggaag cgggtagtgg gggccgtcgc tggtgaccgg
      961 cgcctcgcgg gccaccgtcc gccggacgat gtcgacgtac tcgcgggtcc gggccagcgg
     1021 cttggcaaac gggcggccgt accagccctc ggccacttgg gggccgccga ccccgagccc
     1081 gagcaccgcc cgcccgccgg acaggccgtc gagggtgatg gcgtgcatgg ccgccgtggt
     1141 cgggggccga gccccgatct gggcgatccc ggtgcagagc ctgatcgacg atgtcgcccc
     1201 cgccaccgcg gccagcgggg cgaaggcgtc gctcgaccac gactcccccg tccacagcga
     1261 gtcgtacccg agggcctcgg cccgctgggc ggcggcgatg agatcggtgg cgggccgggg
     1321 ctccgccgtc cagtagccgt actggtaccc gagcttcagc ccgctcacgg gaagaggacg
     1381 tagaccttgc gcagcgtctc gtggatctcc caggtgccgg cccagccctt ggggaacacc
     1441 gccgtcgacc cggccgacag ctcgacctcg gtgccgtcgt cgtggcggca ggtcatccgg
     1501 cccgacaaga cgtggatgaa ctcgccctcc tcgaggtccc agcgggagtt gcccggctcg
     1561 cactcccaga tcccgctgga caccgactcg tcgtcggccg cccagaggtc cagcccgtag
     1621 gtcgccatcg gctcccccag cgccgtggcc aggggcggct cgagcgtccc ccagtcctcg
     1681 aggtcctcgg tggacagttc ggcggcgcgg acgttcggcg tggtcatcgc ccggaccgta
     1741 gccaacccgg cctccgcccg aggccggatc cggaggggtc tagattcacg cgccgtgccc
     1801 tcacccgcgt tcgaccgcga cgatctgctg gccaaggccc aggagcgggc cggcgggctg
     1861 accgatctcg ggcccggccc gttcctcgac gggatggatc gcttcgtcga ctcgctcaac
     1921 gccgaggcca ggctgaacga catcggccgc ttcatcgccg aggagcgggt cctgctccac
     1981 gtcaccaacc gcctgctcta cacccacgac cgtgagcagc acccggagat cgcaagcgtc
     2041 gacgtggtca agcccgtgtt catcatcggc ctgccccgca cgggcaccac gatcctgcac
     2101 gacatcctga accaggaccc ggccaaccgc gccccgctca cctgggagct gatgttcccg
     2161 tccccaccgc ccgaggcctc gatcggcgac gaggaccccc gcgtggcggc gtgccaggcc
     2221 acgatccccg acgacgacct gcagtccacg ctcttcaagg ccatgcaccc gatgggggcg
     2281 accctgtccc aggagtgcgt cgtgatgatg ggcgaggcga tggtcacccc gctgttccac
     2341 aaccagttcc gcgtgcccgc ctaccaggac tgggtggacg acggcgcgga ctggtccgcc
     2401 gtctacgcct tccaccgccg ccagctccag cacctgtggt ggcacaagcc cggcgaccgg
     2461 tggatgctca agacgggcgc ccacatgtgg ggcctcgacc agctgctggc gacctatccc
     2521 gacgcccgca tcgtgttcac ccatcgggac ccggtgaagt cgatgacgtc ctacgccagc
     2581 ctgaccacgc tcgtgcggtc gatgggcagt gacgacgtcg acccccacga gatcgccgcg
     2641 gactggaccg cccggctcaa gcggatgctc gagcgctcga tggcggtgcg gaccgcccgg
     2701 gactacccgg acgccgtctt ctacgacatg tacttctcgg acttcgtggc cgaccagttc
     2761 gccgaggtcg agcggatcta cgacgccctc gacctcccga tgtcggcgga gggggcgacg
     2821 ggcatgcgcc agtacatcgc cgaccacccc aagggggtgg acgggatcca ccgctaccgg
     2881 cccgaggagt acggcgtcga ccccgacacc gtccgccgcg acttcgcccc ctacatcgaa
     2941 cgcttcggtc tcgagcccga ggcccctgaa cccgaggacg gcccatgacc cccacgacga
     3001 tgcgcgcctg gcagacccat gagtacggcg agccggggac cgccctgcac ctcgatgagg
     3061 tcgagatccc cgagccgggc gacggcgagg tccgcgtccg cgcccttggc atcccgctca
     3121 acctgaacga cctcgagcgc atcacgggtg gca
//
LOCUS       DASTFV010000421         2971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1554427, whole genome shotgun sequence.
ACCESSION   DASTFV010000421 DASTFV010000000
VERSION     DASTFV010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554427"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..673)
                     /locus_tag="VFP02_07790"
     CDS             complement(86..673)
                     /locus_tag="VFP02_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET9542968.1"
                     /translation="MSEPIDDPDGQGRAWTRVDRRRVLLAGLATVALLAVLWIEAFSR
                     SDTDEADDAPVGDWPTSPVAEDDVPADLLRTYQEASTNCPGLPWPVVAAIGKAETDHN
                     RSPGTSGSGAVGPMQFLPETWEMFQADGDGDGDADVEDEEDAIAGAVRLLCASGGEAP
                     DTLRAAIFAYNRSDEYVADVLGIARTYTSGTIDAP"
     gene            723..1382
                     /locus_tag="VFP02_07795"
     CDS             723..1382
                     /locus_tag="VFP02_07795"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HET9542969.1"
                     /translation="MALRGFERRLENLVEGTFSRMFRSGLRPVEVGRRLTREMDGARS
                     VDVNGRVVVPNDFTIWLSPGDHERFVQMEQALVLELCEAAREHARDESYGFVGPVRVE
                     LSPWEKLRTGTFQIESKMAEGTGGVGAGSLLLPTNQRIGLGEQPVVIGRAPECNIQLG
                     DTNVSRRHAEIVPRGTGYALVDLNSTNGVRVNGLRVAEQVLIDGDEILIGNTRMIFEA
                     S"
     gene            1383..1847
                     /locus_tag="VFP02_07800"
     CDS             1383..1847
                     /locus_tag="VFP02_07800"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9542970.1"
                     /translation="MSEQLLTILKFALLALLYLFFLRVLRAVWAEIRGPRVSTRRQQA
                     AAVSQVAPSRKARKSGEPHLLVVEPVAQRGRTFPLGQELTVGRAAGCQVTLDDTYVSQ
                     LHARVFARDGQYLVEDLGSTNGTYLNRARVTGSIVMKRGDRLQVGNTVMELA"
     gene            1844..>2971
                     /locus_tag="VFP02_07805"
     CDS             1844..>2971
                     /locus_tag="VFP02_07805"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET9542971.1"
                     /translation="MTTFRAGSATDVGRVRSNNQDSLLVREGDLFAVADGMGGHQGGE
                     VASALALETLGQAHDDPSTSTLVEAVRSANRAVFEKAGTSPDLKGMGTTLTALADVET
                     TEGKRLGIVNVGDSRLYRARGEHIEQLTEDHSLVASLVRQGRITAEEAESHPQRNILT
                     RALGIDEAVAVDSWEVEPIPGDRFLICSDGLFNEVDEGRMIATLRRFDDPADAARELV
                     RLANEGGGRDNITAVVVDVVSTEGGAVAAPPVEGEEGPGRDPTTQPIAAVGPAVPEEE
                     PGASAATLDPEDEVEPPTRSRRFTWRVAVFVVALLLLVALVIGALGWYARNTFFVVID
                     DEEVTIFKGRPGGFLWFDPTVEDRTGISVADVPEAELPDLED"
BASE COUNT          438 a         1053 c         1027 g          453 t
ORIGIN      
        1 caggaacctg aatcctgcgc gtctgccaaa ttccgccact cgcccgagtg gagccgagag
       61 cctacccgcg cgctccagtc gtgccctacg gggcgtcgat ggtgccgctc gtgtaagtac
      121 gggcgattcc gaggacgtcg gcgacgtact cgtcggagcg gttgtaggcg aagatcgcgg
      181 cccggagcgt gtccggcgcc tcgcccccac tcgcgcagag caggcgtacg gccccggcga
      241 tggcgtcctc ctcgtcctcg acgtcggcgt cgccgtcccc gtcgccatcg gcctggaaca
      301 tctcccaggt ctcgggcagg aactgcatgg ggccgacggc accggagcct gacgtgcccg
      361 gggaccggtt gtggtccgtc tccgccttgc cgatggcggc cacgaccggc catggcagac
      421 cagggcagtt ggtggacgcc tcctggtagg tgcggaggag gtcggcgggg acgtcatcct
      481 cggcgacggg gctggtcggc cagtccccga cgggggcgtc gtccgcctcg tcggtgtccg
      541 acctggagaa ggcttctatc cagagcacgg cgagcagcgc gacggtggcc agaccggcca
      601 gcaggacccg tcgtcgatcg acccgcgtcc acgcccgacc ctggccgtcg gggtcgtcga
      661 tgggttcgct cacggtccgc ctcccacggg gaacggggga cggtagcgcc tatgttctct
      721 ccgtggctct gcgagggttc gagcgccgac tcgagaacct ggtcgagggg accttctcgc
      781 ggatgttccg cagcgggctc cgccccgtcg aggtcggccg ccgtctgacc cgggagatgg
      841 acggcgcccg atcggtcgat gtcaacggcc gtgtggtcgt gcccaacgac ttcaccatct
      901 ggctcagccc cggcgatcac gagcgtttcg tccagatgga acaggcgctg gtcctcgagc
      961 tctgcgaggc cgcccgcgag cacgccaggg acgagtccta cggcttcgtc ggccccgtcc
     1021 gggtcgagct ctcgccgtgg gagaagctgc gcaccgggac cttccagatc gagtccaaga
     1081 tggccgaggg cacgggaggg gtcggggcgg gatcgctcct gttgcccacc aaccaacgca
     1141 tcggcttggg cgagcagcca gtcgtcatcg ggcgggcgcc ggagtgcaac atccagctcg
     1201 gcgacaccaa cgtcagtcgg cgccacgccg agatcgtccc ccggggcacc gggtacgccc
     1261 tcgtcgacct caactccacc aacggggtcc gggtcaacgg gctgcgggtg gccgagcagg
     1321 tcctcatcga cggcgacgag atcctgatcg gcaacacccg gatgatcttc gaggcgtcgt
     1381 agatgtccga gcagctcctc acgattctca agttcgcgct cctggcgctg ctctacctct
     1441 tcttcctccg cgtgctgcgg gccgtctggg ccgagatccg cggcccccgg gtctccacgc
     1501 ggcgccagca ggccgccgcc gtcagccagg tggcgccttc tcgcaaggcc cgcaagtcgg
     1561 gcgagcccca tctgctcgtg gtcgagcccg tcgcccaacg gggtcggacc tttccgctgg
     1621 gccaggagct gaccgtcggc cgggcggccg ggtgtcaggt gaccctggac gacacctacg
     1681 tgtcccaact ccatgccagg gtgttcgccc gtgacggcca gtacctggtg gaagacctcg
     1741 gctcgaccaa tggcacctac ctcaaccgcg cccgcgtcac cggctcgatc gtcatgaaac
     1801 ggggcgaccg cctgcaagtc ggcaacaccg tcatggagct ggcgtgacca ccttcagggc
     1861 cggctcggcc accgacgtgg gccgggtccg ctccaacaac caggacagcc tcctggtgcg
     1921 ggagggcgat ctcttcgcgg tcgccgacgg catgggcggc catcagggcg gcgaggtggc
     1981 ctcggccctc gccctggaga ccctgggtca ggcccacgat gatccgagca cctcgacgct
     2041 ggtggaggcc gtccgctcgg ccaaccgggc cgtcttcgag aaggccggga cctcaccgga
     2101 cctgaagggg atggggacca ccctcaccgc cttggccgat gtggagacca ccgagggcaa
     2161 gcggctcggg atcgtcaacg tcggcgactc ccggctctac cgggcccggg gggagcacat
     2221 cgaacagctc acggaggacc acagcctcgt ggccagcctc gtccgacagg gtcggatcac
     2281 cgccgaagag gccgagtccc atccccagcg caacatcctg acccgggcgc tgggcatcga
     2341 cgaggcggtg gccgtcgact cctgggaagt cgaaccgatc cctggcgacc gcttcctgat
     2401 ctgcagtgac ggcctgttca acgaggtcga cgagggtcgg atgatcgcca ccctccgacg
     2461 cttcgacgat cccgcggacg ccgctcgcga actggtgcgg ctggcgaacg aggggggtgg
     2521 tcgcgacaac atcaccgccg tcgtggtgga cgtggtgtcg accgaagggg gtgcggtcgc
     2581 ggcgccgccg gtcgaaggcg aggagggtcc tggtcgcgac ccgaccaccc agcccatcgc
     2641 cgccgtcggg ccggccgtcc ccgaagagga gcctggtgcc tccgccgcca ccctcgaccc
     2701 cgaggacgag gtcgaacccc cgacccgttc ccggcggttc acctggcggg tggcggtgtt
     2761 cgtcgtagcc ctgctcctgc tcgtggccct ggtcatcggc gccttgggtt ggtacgcccg
     2821 caacaccttc ttcgtcgtca tcgacgacga ggaggtgacc atcttcaaag gccgtcccgg
     2881 cggcttcctc tggttcgacc cgacggtcga ggaccgcacg ggcatctcgg tggccgatgt
     2941 gcccgaggcc gaactgcccg acctcgagga c
//
LOCUS       DASTFV010000422         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1558251, whole genome shotgun sequence.
ACCESSION   DASTFV010000422 DASTFV010000000
VERSION     DASTFV010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558251"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /locus_tag="VFP02_07810"
     CDS             <1..1882
                     /locus_tag="VFP02_07810"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9542972.1"
                     /translation="DEDVDELMGPVVEDLAAAGVHVLWPAELMETVELKPVVSTAPAA
                     VTSSGLSLETLLELRWEATVGDEQLTEQELRALAEARRPMVRLRGRWVRADPRALARV
                     LERRRVAAGEALAAALGGTLTVDGQTLEVELEGPLRDLADRLAAMDPEREQRPPSGLV
                     AVLRPYQLRGLSWLSEMADLGVGGVLADDMGLGKTIQVLALHLARRDRGRPTLVVCPA
                     SVLANWERETARFAPSVPTRRFHGPGRDLDGLGGDEIVVATYGVVRRDAEALAAVGWG
                     LVIADEAQAVKNPNARTARALRRIHGDARFALTGTPVENRLTELWAILDWTTPGLLGP
                     LHAFRTDVALPIERYQDEEVADSLRRIVRPFLLRRKKSDPTIAPDLPPKTEIDRIVPL
                     TVEQASLYRAVTDEVLAQIESAAGFARRGLVLKLLTALKQICNHPAQYLGQVAPLDGR
                     SGKLDGATELLEIVTAEDEAALVFTQYVAMGRLLEEHLTGRGLRTFFLHGSLTIKARE
                     ELVARFQAGEADAFVISLRAGGTGINLTRATHVVHYDRWWNPAVEDQASDRAWRIGQT
                     RPVEVHRLVCEGTVEDRIATLLATKRDLAEAVVGAGEAWISDLDDEALAELVELSVGG
                     GS"
     gene            1882..>2114
                     /locus_tag="VFP02_07815"
     CDS             1882..>2114
                     /locus_tag="VFP02_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542973.1"
                     /translation="MAERRVFGTTWWGKAWTDALQRRARLDANRLPRGRTYARHDRVH
                     DIELSKGEINARVQGSRPEPYTVRVHIRRFSDD"
BASE COUNT          290 a          761 c          779 g          284 t
ORIGIN      
        1 ggacgaggac gtggacgagc tgatgggccc ggtcgtcgag gacctcgccg ccgccggcgt
       61 ccacgtgctc tggccggccg agctgatgga gacggtcgag ctgaagcccg tcgtgtcgac
      121 cgcccccgcc gcggtcacgt cgtcgggcct ctcgttggag accctgctcg agttgcgctg
      181 ggaggcgacg gtcggcgatg agcagctcac cgaacaggag ctccgcgccc tggccgaggc
      241 ccggcggccg atggtccgct tgcggggtcg gtgggtgcgg gccgacccca gggcgctggc
      301 tcgggtcctg gagcggcgac gggtggcggc gggcgaggcg ctggccgctg ccctcggcgg
      361 gacgttgacc gtcgacgggc agacgttgga ggtggagctg gagggcccgc tgcgggacct
      421 ggccgaccgg ctggcggcca tggaccccga gcgggagcag cggccaccct ccgggctcgt
      481 cgccgtcctc cggccgtacc agctgcgggg cctgtcgtgg ttgagcgaaa tggccgacct
      541 gggtgtgggt ggcgtcctgg ccgacgacat ggggctgggc aagaccatcc aggtcctggc
      601 gctgcacctg gcccgccggg accgaggccg cccgaccctc gtcgtctgcc cggcgtcggt
      661 cctcgccaac tgggaacggg agacggcccg gttcgccccc agcgtgccga cccgccgatt
      721 ccacgggccc ggtcgggacc tcgacggcct cggcggcgac gagatcgtgg tcgccaccta
      781 cggcgtcgtc cgccgggacg ccgaggcgct ggccgccgtc gggtgggggc tggtcatcgc
      841 cgacgaggcc caggcggtga agaacccgaa cgcccgcacc gcccgggccc tccgtcgcat
      901 ccacggcgac gcccgtttcg ccctcacggg cacgcccgtg gagaaccgac tcaccgagct
      961 ctgggcgatc ctggactgga ccacgcccgg gctgctcgga ccgctccacg ccttccggac
     1021 cgacgtggcg ctccccatcg agcggtacca ggacgaggag gtggcggact cgctccgccg
     1081 gatcgtccgt cccttcctcc tgcgccggaa gaagagcgac cccaccatcg cccccgatct
     1141 gccgccgaag acggagatcg accggatcgt tcccctcacg gtcgagcagg cgtcgctcta
     1201 ccgggcagtg accgacgaag tgctggcgca gatcgagtcg gccgccggct tcgctcgccg
     1261 cgggctggtc ctcaagctcc tgaccgccct caagcagatc tgcaaccacc cggcgcagta
     1321 cctcggccag gtcgcgccgc tcgacggccg gtccggcaag ctcgacgggg ccaccgagct
     1381 gctggagatc gtgaccgcgg aggacgaagc ggccttggtc ttcacccagt acgtcgccat
     1441 ggggcggctc ttggaggagc acctcactgg acgtggcctc cgcaccttct tcctgcacgg
     1501 ctcgctgacc atcaaggccc gggaagagct ggtggcccgc ttccaggccg gcgaggccga
     1561 tgccttcgtc atctcgctga gggcgggggg cacgggcatc aacctgaccc gggccacgca
     1621 cgtcgtgcac tacgaccggt ggtggaaccc ggcggtggag gaccaggcca gcgaccgggc
     1681 gtggcgcatc ggccagaccc ggccggtcga ggtccatcgc ctcgtctgcg agggcacggt
     1741 cgaggaccgc atcgccaccc tgctcgccac caagcgggac ctggccgagg ccgtcgtcgg
     1801 cgcgggtgag gcctggatct ccgacttgga cgacgaagcg ctggccgagc tggtcgagct
     1861 gtccgtcggt ggagggtcct gatggccgag cggcgggttt tcggcaccac gtggtggggg
     1921 aaggcgtgga ccgacgcgct ccagcggcgg gctcgcctcg acgccaaccg tctgccccgg
     1981 ggccggacct acgcccgcca cgaccgggtc cacgacatcg agctcagcaa aggcgagatc
     2041 aatgcccggg tgcagggcag ccggcccgag ccgtacacgg tccgggtcca catccgccgg
     2101 ttcagcgacg acga
//
LOCUS       DASTFV010000423         2330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1561876, whole genome shotgun sequence.
ACCESSION   DASTFV010000423 DASTFV010000000
VERSION     DASTFV010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561876"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VFP02_07820"
     CDS             complement(<1..673)
                     /locus_tag="VFP02_07820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9542974.1"
                     /translation="MTGPHRVGVNLLWLVPGVVGGSEEYTVRTLDGILDVAPPDVDLV
                     LFALRPFIEAHPELTDRIETVTLSLTGRDKSVRVAAESSWLAFQCRRHGVAMVHHAGG
                     ILPAVRAVPGLLTVHDVQPLLQPENFSPAKRAFSRAVLPRSAKAARLVVTPSEYSRQA
                     LLSMVDVPPEHVWIVPHGIPEPSVGPGREVDLAQVEAAFGIKAPFFVYPAITYPHKNH
                     LTLVRA"
     gene            complement(670..1758)
                     /locus_tag="VFP02_07825"
     CDS             complement(670..1758)
                     /locus_tag="VFP02_07825"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET9542975.1"
                     /translation="MRLAVTLEQCWHRVPGGTATSVLRSVEALGRRADVELVGVAARH
                     GHPPAEPYGPPVPVWELGLPRPLLYASWHRLRRPAVERATGPVDAVWASGVAVPPRRV
                     PLIVTVHDLAPLHHPEHHPRRAQGFYRQAFALARTEADLVCCPSQATLEDCAAHGFDR
                     GRLRHVPWGVTPLPVTPHAITEVRTRLGLDEEYVLWTGTIEPRKNLPGLIEAFARIAR
                     PGLLLAVAGPDGWHEDLGALIGERKDIRALGFVSDADLAALYAGAAVFCYPSLLEGFG
                     LPVLEAMAQGAPVVTSAGTATEELVAGVGGAVDPTDVGAVAGALSAVLDDPAEADRRR
                     AAGRVRAAELTWDATAAAMMDVVHEAIA"
     gene            1813..>2330
                     /locus_tag="VFP02_07830"
     CDS             1813..>2330
                     /locus_tag="VFP02_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9542976.1"
                     /translation="MAGYLTEQRVLVTGGSGFLGRAVLERVRAFDPALVAAPGSEEYD
                     LRRADDAVRLFDDVDPDLVIHLAARVGGIGANRERPADLYLDNLLMGTYVLDEARRRG
                     TAKTVMIGTICSYPKLAEVPFSEDQLWTGYPEETNAPYGIAKLAQLAQAQANREQYGQ
                     DVIYLMPTNLYGP"
BASE COUNT          345 a          825 c          872 g          288 t
ORIGIN      
        1 aggcccgcac gagcgtcagg tggttcttgt gggggtaggt gatcgccggg tagacgaaga
       61 acggcgcctt gatcccgaaa gcggcctcga cctgggccag gtcgacctca cgaccgggcc
      121 cgaccgaggg ttccgggatg ccgtggggga cgatccagac gtgctccggg ggcacgtcga
      181 ccattgagag cagggcctgg cggctgtact cgctgggggt gacgaccagt cgggctgcct
      241 tagccgaccg agggagtacg gcccggctga acgcccgctt ggccgggctg aagttctccg
      301 gctgcagcag gggctgcacg tcgtggacgg tcaggagacc gggcaccgcc cggacggccg
      361 ggaggatccc gccggcgtgg tggaccatgg ccacgccgtg gcgacggcac tggaacgcca
      421 gccacgagct ctccgccgcc acccggaccg acttgtcccg tcccgtgagg gacagcgtca
      481 cggtctcgat ccggtcggtc agctccggat gggcctcgat gaacggtcgt agcgcgaaca
      541 ggaccaggtc gacgtcgggc ggggccacat cgaggatgcc gtcgagggtc cggacggtgt
      601 actcctcgct cccccccacc acaccgggca cgagccagag caggttgacg ccgacgcggt
      661 ggggcccggt cacgcgatgg cctcgtgcac gacatccatc atcgccgccg ccgtggcgtc
      721 ccacgtcagc tcggcggccc ggacccgccc ggcggcgcgg cggcggtcgg cctcggccgg
      781 atcgtcgagc acggcggaca gggccccggc caccgcaccg acatcggtcg ggtcgacggc
      841 gccacccacc cccgcgacca gttcctcggt cgccgtcccg gccgaggtga ccaccggcgc
      901 cccctgggcc atcgcctcca ggaccggcag cccgaagccc tcgaggaggc tcgggtagca
      961 gaacaccgcg gccccggcgt agagggcggc cagatcggcg tcgctgacga agccgagggc
     1021 tcggatgtcc ttgcgctcac caatgagcgc ccccaggtcc tcgtgccaac cgtcgggccc
     1081 ggccaccgcg aggaggaggc cggggcgggc gatccgggcg aacgcctcga tgagcccggg
     1141 caggttcttg cggggctcga tggtgccggt ccagaggacg tactcctcgt ccaggcccag
     1201 gcgggtacgc acctccgtga tggcgtgcgg ggtgaccggc aggggcgtga caccccaagg
     1261 cacgtggcgg agccgacccc ggtcgaagcc gtgggcggcg cagtcctcca acgtggcctg
     1321 cgaggggcaa cagaccaggt cggcctcggt ccgggccaga gcgaaggcct gccggtagaa
     1381 gccctgcgcc cggcggggat ggtgctctgg gtggtgcagc ggggcgaggt cgtgcacggt
     1441 cacgatcagg ggcacccggc ggggcgggac ggccacgccg ctggcccaca ccgcatcgac
     1501 cggcccggtc gcccgctcga ccgcaggtcg gcgcagccgg tgccaggacg catagagcag
     1561 cgggcggggc aggcccagct cccagaccgg gacggggggc ccgtacggct ccgcgggtgg
     1621 atggccatgg cgggcggcca cgccgacgag ctcgacgtcg gcgcgccggc ccagcgcctc
     1681 cacggagcgg agaaccgagg tcgcggtccc tccgggcacg cggtgccagc attgttcgag
     1741 ggtcaccgcg agccgcaccg ggcgagtgtt gcaggggttg ggcgaaatgc acgccggtag
     1801 cgtggtgccc cgatggccgg atacctgact gagcagcgcg tcctggtgac cggcgggtcg
     1861 gggttcctcg gtcgagcggt gctcgagcgg gtgcgggcct tcgacccggc tctggtggcc
     1921 gcccccggca gcgaggagta cgacctgcgc cgggccgacg acgccgtgcg cctcttcgac
     1981 gacgtggacc cggacctggt catccacctc gccgcgcggg tcggcggcat cggcgccaac
     2041 cgcgagcgtc cggcggacct ctacctcgac aacctgctca tgggcaccta cgtcctcgac
     2101 gaggcgcggc ggcggggcac ggccaagacg gtgatgatcg ggaccatctg ctcgtatccc
     2161 aagctggccg aggtcccctt ctccgaggat cagctgtgga cgggctatcc cgaggagacc
     2221 aacgccccct acggcatcgc caagctggct caactggccc aggcccaggc caaccgggag
     2281 cagtacggcc aggacgtcat ctacctgatg ccgaccaacc tctacggccc
//
LOCUS       DASTFV010000424         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1563428, whole genome shotgun sequence.
ACCESSION   DASTFV010000424 DASTFV010000000
VERSION     DASTFV010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563428"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFP02_07835"
     CDS             <1..222
                     /locus_tag="VFP02_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9542977.1"
                     /translation="AEVAVHPTDGPPSHELYLMCDDVEATVADLTAAGIEVTKPVEDQ
                     GWGLLTSIRLPGGSELGLYQPRHDRPPQA"
     gene            complement(226..1023)
                     /locus_tag="VFP02_07840"
     CDS             complement(226..1023)
                     /locus_tag="VFP02_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542978.1"
                     /translation="MAIVIRETDGMDEIRSVTALMAEVWGDDFLDTGICRAIQHAGGY
                     LTGAWEGDRLLGGSLAFIGYLDGEVLLHSHATCTAPGLGSQGIGLALKWHQRRWCLDR
                     GIRTVEWTYDPLVARNGWFNLTKLGARAVAYEIDFYGRMTTAIERGEESDRCLVRWTL
                     DDAKVVAAAAGALVGADVEALRAAGVPVLISAGPDGGPVVGDPLSEGPALAQVALDIE
                     ALRVSNPEVAAKWRRAVRATLGHAMRDNRHLTAAARDGWYLLEPTAG"
     gene            complement(1026..2138)
                     /gene="menC"
                     /locus_tag="VFP02_07845"
     CDS             complement(1026..2138)
                     /gene="menC"
                     /locus_tag="VFP02_07845"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HET9542979.1"
                     /translation="MRLHAVDLHRVRLPLVRPFRTSFGEQTVREVLVIQAVTDVGEGW
                     GECAADVDPHYSPEFNDGAHLALIDHLVPMLFAAGDDLSGAAVADLLEPVKGHRMAKE
                     ALEVAVLDAELRAAGVSLAEHLGGVRDAVDVGVSVGITDDPGVLAGWVAEYLDEGYRR
                     IKLKIEPGADIERVAAIRELTGDGFPLQVDANTAYRVTDADHLARLDEFDLLLVEQPL
                     LEDDFVGHAELARRLQTPICLDESIESVAHVETALALGSCGIVNIKPGRVGGLLEARR
                     IHDRCVAADIPVWCGGMLETGIGRASLVALSALPGFTLPGDSSASDRYYATDLTEPFV
                     LDDGRLRVPTGPGLGVEVDGEALARFRVAQDHLTPT"
BASE COUNT          325 a          788 c          826 g          289 t
ORIGIN      
        1 gcggaggtcg ccgtccaccc gaccgacggg ccacccagcc acgagctcta cctgatgtgt
       61 gacgacgtcg aagccaccgt cgccgacctc accgccgccg gcatcgaggt gaccaagccg
      121 gtcgaggacc agggctgggg cctcttgacc agcatccgcc tcccgggcgg cagcgagctc
      181 ggcctctacc aaccccgcca cgaccgccca ccccaggcct gacggtcagc ccgccgtggg
      241 ctccaggagg taccagccgt ctcgggcggc ggcggtgagg tggcggttgt cgcgcatggc
      301 gtggccgagg gtggcgcgga cggcgcggcg ccacttggcc gccacctcag ggttgctgac
      361 gcggagggcc tcgatgtcga gggcgacctg ggccaaggcc ggtccttccg acagggggtc
      421 gccgacgacg gggccgccgt caggtccggc cgagatgagc accgggacgc cggcggcgcg
      481 gagtgcctcc acgtcggctc ccaccagcgc acccgccgcc gcggccacca ccttggcgtc
      541 gtccagcgtc cagcgcacga ggcagcggtc gctttcttcg ccccgctcga tggcggtggt
      601 catgcggccg tagaagtcga tctcgtacgc caccgcccgg gcgccgagct tggtcaggtt
      661 gaaccagccg ttccgggcaa cgagggggtc gtaggtccac tcgacggtgc ggatgccacg
      721 gtcgaggcac caccgccgtt ggtgccactt gagggccagg ccgatgccct ggctgccgag
      781 gcccggcgcc gtgcaggtgg cgtgggagtg gagcaggacc tcgccgtcca gatagccgat
      841 gaaggccagc gagccgccga gcaggcgatc accctcccag gccccggtca ggtagccgcc
      901 ggcgtgctgg atggcccgac agatcccggt gtccaggaag tcgtcacccc acacctcggc
      961 catcagggcg gtcaccgacc ggatctcgtc catgccgtcg gtctcgcgga tgacgatggc
     1021 catggtcagg tcggcgtgag gtggtcctgg gccacgcgga accgggcgag tgcctcgccg
     1081 tcgacctcca cgccgaggcc gggaccggtc ggcacccgca ggcgcccgtc gtcgagcacg
     1141 aagggttcgg tcaggtcggt ggcgtagtag cgatccgacg ccgaagagtc gccgggcagg
     1201 gtgaagcccg ggagggcgga gagcgccacc agggaggccc ggccgattcc ggtctccagc
     1261 atcccgccgc accagaccgg gatgtcggcg gccacgcagc ggtcgtggat gcggcgggcc
     1321 tccagcaggc cgccgacccg gcccggcttg atgttgacga tcccgcacga ccccagggcc
     1381 agcgcggtct cgacgtgggc cacggactcg atggactcgt ccaggcagat cggcgtctgc
     1441 aggcggcggg ccagctcggc gtggccgacg aagtcgtcct cgagcagggg ctgctcgacc
     1501 aggagcaggt cgaactcgtc gagccgggcc aagtggtcgg cgtcggtgac ccggtaggcc
     1561 gtgttggcgt cgacctgcag gggaaagccg tcgccggtga gctcgcggat ggcggccacc
     1621 cgctcgatgt cggcgccggg ctcgatcttc agcttgatgc ggcggtagcc ctcgtcgaga
     1681 tactcggcca cccatccggc cagcacgccg gggtcgtcgg tgatcccgac ggacacgccg
     1741 acatcgaccg catcccgcac ccccccgagg tgctcggcca gcgagactcc cgcggcccgt
     1801 agctcagcgt cgagcacggc cacctcgagg gcctccttgg ccatgcggtg gcccttgacc
     1861 ggctcgagga ggtcggccac ggcggcgccg gacaggtcgt cgccggcggc gaacagcatc
     1921 ggcacgaggt ggtcgatcag cgccaggtgg gcaccgtcgt tgaactcggg ggagtagtgg
     1981 ggatcgacgt cggcggcaca ctcgccccag ccctcgccga catcggtcac ggcctggatc
     2041 accagcacct cacggacggt ctgctcgccg aaggacgtcc ggaagggacg gaccaggggg
     2101 aggcggacgc ggtggaggtc gacggcgtgg agacgcacgg gcggatcatc gcacccgcct
     2161 cggtcactcg cgccgctctt cgtcgccggg cgggcgagtc agccgtggtg ctcccgcagg
     2221 agggggac
//
LOCUS       DASTFV010000425         3357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1569428, whole genome shotgun sequence.
ACCESSION   DASTFV010000425 DASTFV010000000
VERSION     DASTFV010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569428"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..277)
                     /locus_tag="VFP02_07850"
     tRNA            complement(204..277)
                     /locus_tag="VFP02_07850"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(241..243),aa:Val,seq:tac)
     gene            complement(305..838)
                     /locus_tag="VFP02_07855"
     CDS             complement(305..838)
                     /locus_tag="VFP02_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9542980.1"
                     /translation="MRRRIMRLLLGVYRRLPVLGRRWVVRTIAPSFTVGAMAIIERQD
                     GAVLLVRHSYRKRWGVPGGLMKRREHPEVGLRREVQEEVGVEVDLLGEPAVVVDPDPQ
                     RVDLVFRGRLRDETAEARPMSPEIVEVAWFLPDELPELQFETSGALMALARTSRNPQS
                     PPLPSLGLLDRHRREAG"
     gene            complement(855..1604)
                     /locus_tag="VFP02_07860"
     CDS             complement(855..1604)
                     /locus_tag="VFP02_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET9542981.1"
                     /translation="MTEDQLLAVLQDAASAAIEATTLSGNYAMVDQGRGQHVADVAAD
                     DAAVAVLVGAGLGVLSEESGRHHADRPVTAVLDPLDGSSNAARGLPWYATSVCAVDDE
                     GPLAAVVVNQVSGTRYEAVRGGGARRDGEVITPSAVTELGDAMVGLSGLPPGWLGWRQ
                     FRALGAVALDLCAVAEGRLDGYLDCSPSAHGPWDYLGGMLVCQEAGALVEDAAGRDLV
                     VLDHGARRTPIAAGTSALLAEALAARRTFPV"
     gene            1676..2239
                     /locus_tag="VFP02_07865"
     CDS             1676..2239
                     /locus_tag="VFP02_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542982.1"
                     /translation="MAVIDVAGFIADLKDHAVDHGFHVHDERHFVESYSLRQAWEVDL
                     HPEEACGGPLDLHLSLEVDPRVLLAFEDKVLEIPEGDDPPDDYHFPLLFNWALPPLPH
                     PPDLLVLATDLAGVGGPDLPLEVSAIDSFAAVTDAPERSLAIVAKLQLSLAKVFVGQE
                     LLCETLDRCLAVSQFLLEAAPAWLDEV"
     gene            complement(2243..3076)
                     /locus_tag="VFP02_07870"
     CDS             complement(2243..3076)
                     /locus_tag="VFP02_07870"
                     /inference="COORDINATES: protein motif:HMM:NF020919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HET9542983.1"
                     /translation="MTRRPGVLLGVGAVFLLALAGVAIWARSGEDASAGELPAHNGRF
                     HVECAYSHSGPDDPIVFPGEPGASHMHNFFGATSTDAFSDAESLLAGDTTCSTHQDTA
                     AYWAPALFDGEDQIDPISSVAYYRAAPGVDPEDVVPFPVGLMMIGGDATSTEPQSTDE
                     VGWACTVEGRPQVSPPPCTGGVLLQVVFPDCWDGEHLDSPDHRAHVARSTDSGCPGSH
                     PVALPQLEFHVQYPLPTDMSDLHLASGSLLTGHADFFNAWDPDKLASEVEYCIGQDAY
                     C"
     gene            complement(3073..>3357)
                     /locus_tag="VFP02_07875"
     CDS             complement(3073..>3357)
                     /locus_tag="VFP02_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542984.1"
                     /translation="NCFADNEVDGITAPPDIDATVPCDGPQQTFQPELARFLELADAS
                     DNPEAVPYADVDLPDPGEQEEMPDAATAPAEPAGPPRTVDIAAITVPDLP"
BASE COUNT          490 a         1193 c         1195 g          479 t
ORIGIN      
        1 aaaacagcgc gcaagaactc aaaacgccac tgaccaggga aacgcttggt gggcgatact
       61 ggtctcgaac cagtgacgtc cgagtcgggc tactcgccag ttctcgtcgt ttcgctctgt
      121 cttgcggtct ctttgcggac tggctgcgtg ctggtccgcc aaccatcgtt catggcctct
      181 gaacaggggc aacactggat ttgtgggcgc tgagggactc gaacccccga ccccctcctt
      241 gtaagggagg tgctctagcc agctgagcta agcgcccggg gcgctcaggt tagggttcgg
      301 cgggtcatcc ggcttcgcga cggtggcggt cgagcaggcc caacgacggc agcggcgggc
      361 tctgcgggtt cctcgacgtc cgggcgaggg ccatcagggc cccgctggtc tcgaactgga
      421 gctccgggag ctcgtcgggc aggaaccagg ccacctcgac gatctccggc gacatcggcc
      481 gggcctcggc cgtctcgtcc cgcaggcgac cccggaagac gaggtcgacg cgctggggat
      541 cggggtcgac aaccacagcc ggctcgccca gcaggtcgac ctcgacgccg acctcctcct
      601 ggacctcccg ccgcaggccg acctcggggt gctcgcgccg cttcatcagg ccaccgggca
      661 cgccccaccg cttgcggtag ctgtggcgga cgagcaacac ggcgccgtcc tggcgctcga
      721 tgatggccat ggccccgacc gtgaagctcg gcgcgatcgt gcgcacgacc caccgccgcc
      781 cgagaacggg caacctccgg tagaccccca gcaggaggcg catgatcctg cgccgcatcg
      841 gcccagggta cgagtcagac ggggaaggtc cgccgggcgg ccagcgcctc ggccagcagg
      901 gccgatgtgc ccgcggcgat cggcgtgcgc cgggccccgt ggtccaacac caccaggtcg
      961 cggccggcgg cgtcctccac gagcgcaccg gcctcctggc acacgagcat cccgccgagg
     1021 tagtcccacg gcccgtgggc cgaagggctg cagtcgaggt agccgtcgag gcgcccctcg
     1081 gccacggcgc acaggtccaa ggcgacggcc ccgagagccc gaaactggcg ccaacccagc
     1141 catcccggcg gcagcccgga caggccgacc atggcgtcgc ccagttcggt gactgccgac
     1201 ggcgtgatca cctcgccgtc ccggcgggct ccaccgccgc ggacggcctc gtagcgcgtc
     1261 ccactgacct ggttcaccac cacggccgcc agcggacctt catcgtcgac ggcacagacg
     1321 ctggtggcgt accagggcag gccccgggcc gcgttgctgg agccgtccag tggatccagc
     1381 acggcagtga ccggtcggtc ggcgtggtgc cgccccgact cctcgctgag cacaccgagg
     1441 ccggccccta cgaggacggc caccgcggca tcgtcggcgg cgacgtcggc cacgtgctgg
     1501 ccccggccct gatcgaccat ggcgtagttc ccggaaagcg tcgtggcctc gatcgcggcc
     1561 gaggcggcgt cctggaggac ggcgagcagc tggtcctcgg tcatgaccgc cgaccgtacc
     1621 gaccgaggtc gcgccgagtg cgtaggtgtc ggcgacgcgt ggcaggctgt gggccttggc
     1681 cgtcatcgat gtcgcggggt tcatcgcgga cctcaaggac cacgcggtgg accacgggtt
     1741 ccatgtgcac gacgagcggc acttcgtcga gtcctactcc ctccgccagg cgtgggaggt
     1801 ggatctccac cccgaggagg cctgcggcgg ccccctggac cttcaccttt ccctggaggt
     1861 cgacccccgg gtgctgctgg ccttcgagga caaggtcctg gagatccccg agggcgacga
     1921 cccacccgac gactaccact tccccctcct gttcaactgg gccctgccgc cccttcccca
     1981 cccgcccgac ctcctggtcc tggccaccga cctggccggt gtgggcgggc ccgacctccc
     2041 tctcgaggtc tccgccatcg actcgttcgc ggcggtgacc gatgcccccg agcgcagcct
     2101 ggccatcgtg gccaagctcc agctcagcct ggccaaggtc ttcgtgggcc aggagctgct
     2161 gtgcgagacc ctggaccgct gcctggccgt cagccagttc ctgctcgagg ccgccccagc
     2221 ctggctcgac gaggtctgac gatcagcagt aggcgtcctg gccgatgcag tactcgactt
     2281 cggaggccag cttgtcgggg tcccaggcgt tgaagaagtc ggcgtggccg gtgagcagcg
     2341 agcccgaggc caggtgcagg tcgctcatgt cggtcggcag cggatactgc acgtggaact
     2401 ccagctgcgg cagggcgacg ggatgggagc ccggacatcc ggagtccgtc gagcgggcga
     2461 cgtgggcccg gtggtccggc gagtccaggt gctcgccgtc ccaacagtcg gggaacacga
     2521 cctggagcag gaccccgccg gtgcacggag gtggggacac ctgcggccgc ccctcgaccg
     2581 tgcaggccca accgacctca tcggtcgact ggggctcggt gctggtggcg tcgccgccga
     2641 tcatcatcag accgaccggg aacggcacca cgtcctcggg atcgacgccg ggggccgccc
     2701 ggtagtaggc caccgaggag atcggatcga tctggtcctc accgtcgaac agggcggggg
     2761 cccaataggc ggccgtgtcc tgatgggtcg agcaggtcgt gtcgccggcc agcagggact
     2821 cggcgtcgga gaaggcatcg gtcgaggtcg caccgaagaa gttgtgcatg tgcgatgccc
     2881 cgggctcacc ggggaacacg atggggtcgt ccggaccgct gtggctgtag gcgcactcga
     2941 cgtggaagcg gccgttgtgc gccggcagct cgccggccga cgcgtcctcg cccgaccggg
     3001 cccagatcgc gacgcccgcg agcgcgagaa ggaacaccgc ccccaccccc agcaggacgc
     3061 cggggcgtcg ggtcacggca ggtcggggac cgtgatggcg gcgatgtcga cggtgcgggg
     3121 cgggcccgcg ggctcggccg gcgcggtggc cgcgtcgggc atctcctcct gctcaccggg
     3181 atcgggcagg tcgacgtccg cgtacggcac ggcctcgggg ttgtcgctgg cgtcggccag
     3241 ttcgaggaag cgggcgagct ccggctggaa ggtctgctgc gggccgtcgc agggcaccgt
     3301 ggcgtcgatg tcgggcggag ccgtgatccc gtccacctcg ttgtcggcga agcagtt
//
LOCUS       DASTFV010000426         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1572969, whole genome shotgun sequence.
ACCESSION   DASTFV010000426 DASTFV010000000
VERSION     DASTFV010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572969"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VFP02_07880"
     CDS             complement(<1..668)
                     /locus_tag="VFP02_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9542985.1"
                     /translation="MLGYLLRAVGVGAVGALAGVALGLGPALTGNVAGAAVGAAIGFA
                     LTSGVYLVTAHRAIHRLRTQVEEMETRDPLTGLRNLRALRTWLEHHLPRANEVQSHTA
                     LLVIHAEGIDRINESHGREIGDELLMALVARVRSCLLDKDQVFRAGGTDIAVVRPDLA
                     GTHAAEELAETIMGLLANPYEVDGERLRVPVCMGLAVAGGRPTNADDMLRDAEVATYR
                     ATTTG"
     gene            complement(797..1222)
                     /locus_tag="VFP02_07885"
     CDS             complement(797..1222)
                     /locus_tag="VFP02_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9542986.1"
                     /translation="MVRRSDYLQRLAAVPLFADLDRHELEAVAEVGTDVDVEENRELV
                     RQGDPASEAFLVLDGTAVATRDGEELATFGPGDFFGEMALLVHGHRSATVTATSPMSV
                     RAFHKTEFDSLLDRSPKVAVKILRTTAVRLLNAEDSPRH"
     gene            1319..2209
                     /locus_tag="VFP02_07890"
     CDS             1319..2209
                     /locus_tag="VFP02_07890"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HET9542987.1"
                     /translation="MASQHSVRRDAPSALSPVRGLAAVAVVALAAVLAFTALTAGAET
                     MVHPGPPSLEGAQNALADAQAARADAIQRRAQGQADLAAARQEISALGLEQAALAHEL
                     EVARHRVRVVTVRAYTGGAGARELDVLMDATDIGEHLYRSELMTGNVVDTEQAIEEYQ
                     ALRAQADDHVIVLAERVDDLQAGIEQAGADIALAAEDIRRAEVELSAAEAMDGASRSD
                     PGEGAWSRLRFCESGGVYTTNTGNGFYGAYQFDLRTWQTMGGTGRPSTAPYWEQDMRA
                     KALFQVRGDQPWPICGRFVG"
BASE COUNT          294 a          785 c          845 g          302 t
ORIGIN      
        1 ccggtggtgg tggcccggta ggtggccacc tcggcgtcgc gcagcatgtc atcggcgttg
       61 gtcgggcgcc cgccggcgac ggccaggccc atgcagacgg ggacccgcaa ccgctcgccg
      121 tcgacctcgt aggggttggc caacaggccc atgatcgtct cggccagctc ctcggccgcg
      181 tgggtgcccg ccaggtcggg acggaccacg gcgatgtcgg tgccgccggc ccggaacacc
      241 tgatccttgt ccagtaggca ggagcggacc cgggcgacca gggccatgag cagctcgtca
      301 ccgatctcgc gcccgtggct ctcgttgatc cggtcgatgc cctcggcgtg gatgacgagg
      361 agggcggtgt gggactggac ctcgttggcg cgcgggaggt ggtgctccag ccacgtgcgc
      421 agcgcccgga ggttgcgcag cccggtgagg gggtcgcgcg tctccatctc ctcgacctgg
      481 gtccgcaggc ggtggatggc gcggtgggcg gtcacgaggt agacgccgct ggtcagcgcg
      541 aagccgatgg cggcgccgac ggctgcgccg gcgacgttgc cggtgagcgc cggccccagc
      601 cccagggcca cgccggcgag ggcgccgacg gcaccgaccc cgacggcgcg cagcaggtag
      661 cccagcacgc tcgcgtcctg tgtctccctg ctggctgcgc tcatgtctgt gccccgcatc
      721 gcgtcgacgg cctccccgat tctccatcgg agcgagcggc ggcgatgtga gggccgggaa
      781 gacggtcggg gacggctcag tggcggggcg agtcctcggc gttcaggagg cggacagccg
      841 tcgtgcgcag gatcttcacc gccaccttgg gcgagcggtc caggaggctg tcgaactcgg
      901 tcttgtggaa ggcccgcacc gacatcggcg aggtcgccgt gacggtcgcc gagcggtgac
      961 cgtggacgag cagagccatc tcgccgaaga agtcgcccgg cccgaaggtg gccagctcct
     1021 cgccgtcgcg ggtcgccacc gcggtgccgt cgaggacgag gaacgcctcg ctggccgggt
     1081 cgccctggcg gacgagctcc cggttctcct cgacgtcgac gtccgtgccg acctcggcca
     1141 ccgcttcgag ctcgtggcgg tccaggtccg cgaacagtgg cacggcggcc agtcgctgca
     1201 ggtagtcgct tcgacgcacc attcgcccct ccaggtcccc ccgagtgggc gcagcgtagc
     1261 ccggtgcccc gatcgggtcc gttgagcggc cgtgggatac gctgccccgg atctcgtaat
     1321 ggcttcgcaa cattcagtcc gtcgtgacgc gcccagcgcc ctgtcccccg tccggggcct
     1381 ggccgccgtg gcggtggtcg ccctcgctgc ggtcctcgcg ttcaccgccc tgacggccgg
     1441 ggcggagacc atggttcacc cggggccgcc ctccttggag ggggcccaga acgccctggc
     1501 ggacgcgcag gcggcccggg ccgatgcgat ccagcgccgg gcccagggcc aggccgacct
     1561 tgccgccgcc cgccaggaga tcagcgccct cggcctcgag caggcggcgc tggcccacga
     1621 gctggaggtg gcccgccacc gggtccgggt ggtcacggtc cgggcgtaca ccggtggggc
     1681 gggcgcccgc gagctcgacg tcctgatgga cgccaccgac atcggcgagc acctctaccg
     1741 cagcgagctg atgaccggga acgtggtgga caccgagcag gcgatcgagg agtaccaggc
     1801 gttgcgggcc caggccgacg accacgtcat cgtgctggcc gagcgggtcg acgacctcca
     1861 ggccggcatc gagcaggccg gggccgacat cgccctggcc gccgaggaca tccgccgggc
     1921 cgaggtcgag ctcagcgcgg ccgaggcgat ggacggcgcc agtcggagcg atccgggcga
     1981 aggggcgtgg agccgcctcc gcttctgtga gtccggtggc gtgtacacga ccaacacggg
     2041 caacgggttc tacggcgcgt accagttcga cctccggacc tggcagacca tgggggggac
     2101 ggggcggccc tccaccgccc cgtactggga acaggacatg cgggccaagg ccctcttcca
     2161 ggttcggggc gaccagccgt ggcccatctg cgggcggttc gtgggctagg accgggacgg
     2221 cggggc
//
LOCUS       DASTFV010000427         2899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1573111, whole genome shotgun sequence.
ACCESSION   DASTFV010000427 DASTFV010000000
VERSION     DASTFV010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573111"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFP02_07895"
     CDS             <1..429
                     /locus_tag="VFP02_07895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9542988.1"
                     /translation="HRGSEENQAGWEHHEADLVDPDSGLMDTLPHFRRTIFGADLEDA
                     VIAIIGDSPTVAAHWTTPLGFLFIDGGHGPEPAHRDYESWVPHVVVGGTLAIHDVFPD
                     PAEGGRPPFELYSRALESLAFEETGATGSLRVLRRVAEGI"
     gene            432..962
                     /locus_tag="VFP02_07900"
     CDS             432..962
                     /locus_tag="VFP02_07900"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9542989.1"
                     /translation="MAEGWDWMRVLTEPCPHCGFDASAVAPDDLAAALREQAAAWESL
                     LASSEDTRLRARPADGTWTALEYGCHVRDLLSVMTERVARTLVEDGPEYGWWDHEASA
                     VDEHYNEQDPVAVAGDLAANAEWMAGTLTLVEGAEWDRPGTRRGADPFTVAGLGRFTL
                     HELAHHRGDAEARLAS"
     gene            complement(977..2065)
                     /locus_tag="VFP02_07905"
     CDS             complement(977..2065)
                     /locus_tag="VFP02_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542990.1"
                     /translation="MPVSPDPRARLEVPGILSAGDIAETAESIAEWQLPTGMIPWFPG
                     GHADVWNHIEATMALDICGRRHEVERAFTWLADTQREDGSWHQYYLADRVEQEKLDAN
                     TVAYVAAGVWHHYLLTEDRGFLEATWGMVERAVNFVMDLQTPRGEILWARHADGTPWS
                     FALLTGSSSISHSLRCALAIAATLGRERPDWELSAARLVHTLRNEPDAFAPKHRWAMD
                     WYYPVLAGVFGGDEGAAHLDERLDTFYLEGRGIRCVSDRPWITAAETCECLLAYLSVG
                     RTDFALELFRQAQTLRSEDGYYWTGIVYPEQVHFPGDERSTYTAAAVILAADALAQSS
                     PASRLFTDHSFLPEAALDETHEDEPSLD"
     gene            complement(2049..2828)
                     /locus_tag="VFP02_07910"
     CDS             complement(2049..2828)
                     /locus_tag="VFP02_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9542991.1"
                     /translation="MLTVDYDRLGVRAGELLLDLGAGNGRHAYEAFRRGVRVVAADFN
                     HADMVECRTMGAAMEAVGEAPAGGLAAAAVADGQLLPFPDDTFDRIIAAEVMEHIPDD
                     EVAATELARVLKPGGTIAITVPSWLPEVVCWKLSDEYHAPKVVGGHVRIYTERELRQK
                     LAATGLEPGGSHQVHALHTPYWWLRCLVGPQEDSNPLVKAYHRLLVWDMVKAPLVTRL
                     AERLLQPVLGKSLVLYARKPTVAVVESRPVPASMEDHARVA"
BASE COUNT          410 a          989 c         1089 g          411 t
ORIGIN      
        1 caccgcggct cggaggagaa ccaggccggt tgggagcacc atgaggccga cctggtcgat
       61 cccgacagcg ggctgatgga cacgctgccc cacttccgcc gcaccatctt cggcgccgac
      121 ctggaggacg ccgtcatcgc gatcatcggt gactccccga cggtggccgc tcactggacc
      181 acgcccctgg gcttcctgtt catcgacggt ggccacggcc cggagccggc ccaccgggac
      241 tacgagagct gggtccccca cgtggtggtg ggtgggaccc tggccatcca cgacgtcttc
      301 cccgatcccg ccgagggggg tcggcccccg ttcgagctct acagccgggc cctcgagtcg
      361 ctcgccttcg aggagaccgg ggccaccggc tcgttgcggg tcctgcgccg cgtggccgag
      421 ggcatctagc catggccgaa ggctgggact ggatgcgggt cctgaccgag ccctgcccgc
      481 actgcgggtt cgacgcctcg gccgtggccc ctgacgacct ggctgcggcg ctgcgagagc
      541 aagccgccgc ctgggagagc ctcctggcct catccgagga caccaggctg cgggcccggc
      601 ccgcggacgg gacctggacg gccctcgagt acggctgcca cgtccgggac ctgctctcgg
      661 tcatgaccga gcgggtggcc cggacgctgg tcgaagacgg ccccgagtac ggctggtggg
      721 accacgaggc ctcagccgtc gacgagcact acaacgagca ggaccccgtc gccgttgccg
      781 gtgacctcgc cgccaatgcc gagtggatgg ccgggaccct gaccttggtc gagggcgccg
      841 agtgggaccg gccgggcacc cgccggggcg cggacccctt caccgtggcc gggctcggcc
      901 ggttcaccct gcacgagctg gcccaccatc gcggcgacgc tgaggcccgc ctggcgtcct
      961 gaccggtgcc cgtcggtcag tcgagggagg gctcgtcctc gtgcgtctcg tcgagggcgg
     1021 cctcgggcag gaagctgtgg tccgtgaaca ggcgggaggc gggcgaggac tgggccaacg
     1081 catcagcggc caggatcacg gcggcggccg tgtaggtcga gcgctcgtca ccggggaagt
     1141 ggacctgttc ggggtagacg atgccagtcc agtagtagcc gtcctcggag cgcagggtct
     1201 gggcctggcg gaacagctcg agggcgaagt cggtgcggcc cacggacagg taggccagga
     1261 ggcactcaca ggtctcggcc gcggtgatcc aggggcggtc cgacacgcag cggatgcccc
     1321 ggccctcgag gtagaaggtg tcgagccgtt cgtcgaggtg ggctgccccc tcgtcgccgc
     1381 cgaagacgcc ggccagcacc gggtagtacc agtccatggc ccagcggtgc ttgggggcga
     1441 aggcgtcggg ctcgttgcgc agggtgtgga ccaggcgggc cgccgacaac tcccagtcgg
     1501 gccgctcgcg gcccagcgtg gcggcgatcg ccaaggcgca gcggaggctg tgcgagatcg
     1561 aggacgaccc ggtcagcagc gcgaaggacc acggggtgcc gtcggcgtgg cgggcccaga
     1621 ggatctcccc acggggggtc tgcaggtcca tgacgaagtt cacggctcgc tcgaccatgc
     1681 cccaggtggc ctccaggaag ccccggtcct cggtcagcag gtagtggtgc cacacgccgg
     1741 ccgccacgta ggcgacggtg ttggcgtcca gcttctcctg ctcgacccgg tcggccaggt
     1801 agtactggtg ccacgagccg tcctcccgct gggtgtcggc cagccaggtg aaggcccgct
     1861 cgacctcgtg gcggcggccg cagatgtcca gggccatggt ggcctcgatg tggttccaga
     1921 cgtcggcgtg gccgccgggg aaccagggga tcatgccggt cggcagctgc cactcggcga
     1981 tggactcggc ggtctcggcg atgtcgccgg cactcaggat gccggggacc tcgaggcgcg
     2041 cgcgaggatc aggcgacacg ggcatggtcc tccatgctgg ctgggacggg ccgcgattcc
     2101 accacggcga cggtcggctt gcgggcgtac aggacgaggc tcttacccag gacgggttgc
     2161 aggagtcgct ccgccagtcg cgtgacgagg ggggccttga ccatgtccca caccaggagg
     2221 cggtggtagg ccttcaccag cgggttgctg tcctcctggg gtccgaccag gcaccggagc
     2281 caccagtagg gggtgtgcag ggcgtggacc tggtgggagc cacccggttc gaggccggtg
     2341 gcagccagct tctggcgcag ctcccgctcg gtgtagatgc ggacgtggcc gccgaccacc
     2401 ttgggggcgt ggtactcgtc ggacagcttc cagcagacga cctcgggcag ccaggacgga
     2461 acggtgatgg cgatggtccc gcccggcttg aggacccggg ccagctcggt ggcggccacc
     2521 tcgtcgtcgg ggatgtgctc catgacctcg gcggcgatga tccggtcgaa cgtgtcgtcg
     2581 ggaaagggca ggagctggcc gtcggcgacc gcggcggcgg ccagcccgcc ggcgggagcc
     2641 tcgcccaccg cttccatggc cgcacccatc gtccggcact ccaccatgtc ggcgtggttg
     2701 aagtcggcgg ccacgacccg aacgccccgg cggaaggcct cgtaggcgtg acgaccgttg
     2761 cccgcgccga ggtccagcag gagctcaccg gcgcgcacgc caagacggtc gtagtcgacg
     2821 gtcagcaccc ggtcagccct gcttgaggcg gatgcggtac tgctcgaccg tcttctcggc
     2881 ggtgtggcgc cacgtccag
//
LOCUS       DASTFV010000428         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1574621, whole genome shotgun sequence.
ACCESSION   DASTFV010000428 DASTFV010000000
VERSION     DASTFV010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574621"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFP02_07915"
     CDS             <1..191
                     /locus_tag="VFP02_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9542992.1"
                     /translation="ADPDLLIELMRQAFGNRRKMLRRSLDGRVTEAQFVTAGVASDTR
                     PEQLDVAAWGRLAEAVGS"
     gene            188..940
                     /locus_tag="VFP02_07920"
     CDS             188..940
                     /locus_tag="VFP02_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HET9542993.1"
                     /translation="MTDALPVVLAAPAKLTLSLRVVGVREDGLHRIDAEMVTVDLVDT
                     LRIGKGDGVTFVGPHAEGLEPGRDLVTRALALVGVTGRVEVDKHIPSGAGLGGGSADA
                     AAILRWVGFDDVEATARLGADVPFCVLGGRARVTGFGEGLQPLPHEDRTFTLLTPPRH
                     CATAAVYQAWDDLGGPEGPNGNDLEPAALAVLPDLARWRDELEEAAGAVPMLAGSGST
                     WFVEGSFPGVGRRVVHTLDSPLRSGRSAPGGS"
     gene            complement(952..1050)
                     /locus_tag="VFP02_07925"
     CDS             complement(952..1050)
                     /locus_tag="VFP02_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HET9542994.1"
                     /translation="MGSLIKKRRKRMRKKKHKKMLKRTRWQRRAGK"
     gene            complement(1132..>2142)
                     /gene="mmsA"
                     /locus_tag="VFP02_07930"
     CDS             complement(1132..>2142)
                     /gene="mmsA"
                     /locus_tag="VFP02_07930"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating, NAD) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET9542995.1"
                     /translation="LAIACGNTFILKPSEKDPAANVRLAELFTEAGLPPGVLNVIHGD
                     AVAVNRLLEHPDIEAVSFVGSTPIARHVYDTGTRSGKRVQALGGAKNHMVVLPDADIG
                     QAADAAVSAGYGSAGERCMAISVVVAVGDVGDRLVPAITERIDQLQIGQGTEDGVDMG
                     PLVTEAHRDRVRSYVDLGVTEGAELVVDGRDLAVDGAEDGFWLGPCLFDRVTPQMKVY
                     LDEIFGPVLSVVRVDTFDEALALVNACEYGNGTAIFTNDGGAARTYHRDVQVGMVGIN
                     VPIPVPMAYFSFGGWKASLFGDTHVHGQEGVRFYTRAKAVTVRWPDPHDRGVDLGFPQ
                     TH"
BASE COUNT          281 a          725 c          804 g          332 t
ORIGIN      
        1 acgccgatcc cgacctcctc atcgagctga tgcgtcaggc gttcggcaac cggcggaaga
       61 tgctgcgccg gtcgctggac ggtcgggtca cggaggcgca gttcgtcacg gctggcgtcg
      121 cctcggacac ccgacccgag cagttggacg tcgccgcctg gggccggctg gccgaggcgg
      181 ttggctcgtg accgatgccc ttcctgtcgt cctggcggcc ccggcgaagc tgaccctctc
      241 cctgcgggtg gtcggcgtgc gggaggacgg tctccaccgg atcgacgccg agatggtgac
      301 cgtcgacctg gtggacaccc tgcgcatcgg gaagggcgac ggggtgacct tcgtcggtcc
      361 ccatgccgaa ggcctcgagc ccggcaggga cctcgtcacc cgggccctgg cgctggtagg
      421 cgtgaccggt cgcgtggagg tggacaagca cattcccagc ggggccgggc tgggtggggg
      481 atcggccgat gccgcggcca tcctgcggtg ggtcgggttc gacgacgtcg aggccacggc
      541 ccgcctcggt gcggacgtcc cgttctgtgt cctcgggggc cgggctcggg tcaccggttt
      601 cggcgagggg ctgcagccgt tgcctcacga ggaccgcacg ttcacgttgc tgacgccgcc
      661 gcgccactgc gccacggcgg cggtctacca ggcgtgggac gacctgggcg ggccggaggg
      721 gccgaacggc aacgacctgg agcccgccgc gttggccgtc ctgccggacc tggcgcgatg
      781 gcgcgacgag ctcgaggaag ctgctggtgc cgttccgatg ctcgccggca gtggttcgac
      841 gtggttcgtc gaaggatcct tcccgggggt gggtcgccgc gtggtgcaca cgctggactc
      901 cccgctccgc tcaggccgat cggcccctgg cgggagctga gcgagctggc gctacttgcc
      961 cgcccggcgc tgccagcggg tgcgcttcag catcttcttg tgcttcttct tgcgcatgcg
     1021 cttgcggcgc ttcttgatca gcgatcccat gtcggcgccg aggctaccgg ccagggaccg
     1081 actcggggcc gatgacgacc gtcaccccgc cgctcatcgg ggtcgcccag gtcagtgggt
     1141 ctgggggaac ccgaggtcga cgccccggtc gtgagggtcg ggccagcgca cggtgacggc
     1201 cttggcccgg gtgtagaagc ggacgccctc ctggccgtgt acgtgggtgt caccgaagag
     1261 cgacgccttc cagcccccga acgagaagta ggccatcggc acggggatgg ggacgttgat
     1321 cccgaccatg cccacctgca catcgcggtg gtaggtccgg gccgcgcccc cgtcgttggt
     1381 gaagatggcg gtgccgttgc cgtactcgca ggcgttgacc aaggcgaggg cctcgtcgaa
     1441 ggtgtcgacc cgcacgacgc tgagcaccgg gccgaagatc tcgtcgaggt agaccttcat
     1501 ctggggggtc acccggtcga acaggcaagg cccgagccag aagccgtcct cggcgccgtc
     1561 gaccgccagg tcccgaccgt ccacgaccag ctcggcgccc tcggtgacgc cgaggtcgac
     1621 gtaggagcgg acccggtcgc ggtgggcctc ggtcaccagc gggcccatgt cgacgccgtc
     1681 ctcggtgccc tggccgatct gcagctgatc gatgcgctcg gtgatcgcgg ggacgaggcg
     1741 gtcgcccacg tcgccgacgg cgaccaccac ggagatggcc atgcagcgct cgcccgccga
     1801 cccgtagccc gcgctcaccg cggcgtccgc ggcctggccg atgtcggcgt cggggagcac
     1861 gaccatgtgg ttcttggccc cgcccagggc ctggacccgc ttccctgacc gggtaccggt
     1921 gtcgtagacg tggcgggcga tcggcgtgga gccgacgaag ctcacggcct cgatgtcggg
     1981 gtgctcgagc aggcggttga cggcaacggc gtcaccgtgg atgacgttga gcacgcccgg
     2041 cgggaggccg gcctcggtga agagctcggc cagacggacg ttggcggccg ggtccttctc
     2101 ggacggcttg aggatgaacg tgttgccgca ggcaatggcc ag
//
LOCUS       DASTFV010000429         2958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1584522, whole genome shotgun sequence.
ACCESSION   DASTFV010000429 DASTFV010000000
VERSION     DASTFV010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584522"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VFP02_07935"
     CDS             <1..966
                     /locus_tag="VFP02_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9542996.1"
                     /translation="PLPPPSADDPRRAINAVRQEVGKVVVGQEGTLSGIVAALLVGGH
                     VLLEGVPGVAKTLLVKAVAGALDLEFKRLQFTPDLMPSDVTGQLVLDADRSFRFRPGP
                     VFTNLLLADEINRTPPKTQASLLESMEERQVSVEGDAHRLPDPFAVIATQNPVEYEGT
                     YPLPEAQLDRFLFKLQVGYPTAEQEQEVLHRHDLGLDPHDLAASGLAPVANAEHLAEG
                     RRQVDALRVEDPVLAYVVAIVRATRESPSLALGVSPRGGASLLKAAKAWAWLAGRDYV
                     TPDEVKAVALPALRHRLLLRPELVLEGVTADGVLQSILATVPAPR"
     gene            980..2287
                     /locus_tag="VFP02_07940"
     CDS             980..2287
                     /locus_tag="VFP02_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9542997.1"
                     /translation="MPIPTRRLAAVLAVASLAVLVLPDRGLTSLLLVDGVVLTIALVD
                     LLLTPAPGRIEVARDLPGVVSLGAEATIAWRLRSSSRRRVWVLVADELAPSLRAGDRR
                     VRIRLEPGEEVRASTTFRPARRGRFQPREVVVRVTGPLGLVSRQRARVLPGLLRVHPP
                     FRSKEEAELRIDRARLLEVGLRSAAGHGGGTEFDQLRDYNVDDEFRRIDWSASARVER
                     PIVRTYRAERNQTVIVLLDNGRVMAGQVGDVPRVEHAMDAVMMLTAVATRLGDRAGLV
                     AFDHEVRAVVSPGHGPGQLGRVTEAMYRLEPALVESDYRGAFTATLARFRRRALLVVL
                     TELVEQAVGEFLAPALPLICARHLVVIGAVQDPEVLAWAKSTPTGPEEAYRKASAVAA
                     LQERNRTIARLQGLGATVVDAAPGRLAPQLADAYLKVKATGRL"
     gene            complement(2259..>2958)
                     /locus_tag="VFP02_07945"
     CDS             complement(2259..>2958)
                     /locus_tag="VFP02_07945"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HET9542998.1"
                     /translation="VGAWIGTSDEALDAAAPDAVREAYLDEGFEAYYSSEPAAQFATE
                     VTVNNIQVSILAFAAGIAFCLGAAYVLILNGANLGVAAGLFVAAGQSPKFFGLILPHG
                     LLELTAISLAAAAGIQMGWALITPGDRTRGDAVAEEGRRSVVIVLGLALAFIVAGTIE
                     GFVTGSGLSTLVRVGIGATVEIAFVLYVVVQGRRAAAMGETGLLGERPPTWGDEWSRP
                     RSASTPTTVLSPSP"
BASE COUNT          381 a         1101 c         1074 g          402 t
ORIGIN      
        1 ccgctcccgc cgccgagcgc cgatgacccc cgccgggcga tcaacgccgt ccgacaggag
       61 gtgggcaagg tcgtggtcgg ccaggagggg acgctgtcgg gcatcgtcgc cgccctgctg
      121 gtgggcgggc acgtgctcct ggagggcgtg ccgggtgtgg ccaagacctt gctggtcaag
      181 gccgtcgccg gggccttgga cctggagttc aagcgcctgc agttcacgcc ggacctgatg
      241 ccctccgacg tcaccggcca gctcgtgctc gacgccgatc gcagcttccg cttccggccc
      301 ggcccggtct tcaccaacct cctgctggcc gacgagatca accggacgcc gcccaagacc
      361 caggcctcgc tcctggagtc catggaggag cgccaggtca gcgtggaggg tgacgcccac
      421 cgcctccccg acccgttcgc ggtcatcgcc acccagaacc cggtggagta cgagggcacc
      481 tacccgctac ccgaggccca gctcgaccgg ttcctgttca agctgcaggt gggctatccg
      541 accgccgagc aggaacagga ggtcctccac cgccacgatc tcggcctcga tccccacgac
      601 ctggccgcgt cggggttggc accggtggcc aacgccgagc acctggcgga gggccgccga
      661 caggtggacg cgctccgggt ggaggatccg gttctggcct acgtcgtcgc catcgtgcgg
      721 gcgaccaggg agtcgccgtc gctggccctc ggcgtgtccc cccggggcgg cgccagcctg
      781 ctgaaggcgg ccaaggcgtg ggcctggctg gccggccggg actacgtcac gccggatgag
      841 gtcaaggccg tggcgttgcc ggccctgcgc caccgcctcc tcctccgccc cgagctggtg
      901 ctggaggggg tcaccgccga cggggtgctg cagagcatcc tggccaccgt ccccgcccct
      961 cgctagccga cctcgtcccg tgccgatccc cacccgccgc ctggcggccg tgctggccgt
     1021 ggcgtcgctg gcggtcctgg ttctgcccga ccggggcctc acgtcgctgc tcctcgtcga
     1081 cggtgtcgtt ctgaccatcg cgctggtcga cctgctgctg acccccgcgc cgggacggat
     1141 cgaggtcgcc cgggacctgc ccggtgtcgt gtccctgggg gccgaggcga ccatcgcctg
     1201 gcggctgcgc agcagctccc gtcgtcgggt gtgggtgctg gtggccgacg agctggcccc
     1261 gtcgctgcgg gccggggacc gtcgggtgcg catccgcctc gaaccgggcg aggaggtgcg
     1321 ggcgtcgacc accttccgcc ccgctcgtcg gggacgcttc caaccgcgcg aggtcgtggt
     1381 ccgggtgacc ggccccctcg gcctggtgtc ccgccagcgg gcccgggtcc tgcccggcct
     1441 gctccgggtc cacccgccgt tccggtccaa ggaggaggcc gagctgcgca tcgaccgggc
     1501 ccgcctgctc gaggtgggcc tgcgctcggc agccggccac ggcgggggca ccgagttcga
     1561 ccagctccgg gactacaacg tcgacgacga gttccgtcgc atcgactggt cggccagcgc
     1621 ccgcgtcgag cgcccgatcg tccgcaccta ccgggccgaa cggaaccaga cggtcatcgt
     1681 gctgctggac aacggccggg tcatggcggg ccaggtgggc gacgtccccc gggtcgagca
     1741 cgcgatggac gcggtgatga tgctgaccgc cgtagccacc cggctgggcg accgggccgg
     1801 gctcgtcgcc ttcgaccacg aggtccgggc cgtggtcagc cccggtcacg gccccggcca
     1861 gctgggccgg gtgaccgagg ccatgtaccg gctggagccc gctctggtcg agagcgacta
     1921 ccggggcgcc ttcacggcca cgttggcccg gttccggcgc cgggccctgc tggtcgtgct
     1981 caccgagctg gtcgagcagg ccgtggggga gttcctggcc ccggccctgc ccctgatctg
     2041 tgcccgccac ctcgtggtca tcggcgccgt gcaggacccc gaggtgctgg cctgggccaa
     2101 gtccacgccg acgggcccgg aggaggccta ccgcaaggcg tcggcggtgg ccgccctcca
     2161 ggagcggaac cgcaccatcg cccggctcca gggcctcgga gccaccgtgg tcgacgccgc
     2221 ccccggccgc ctcgctcccc agctggccga tgcctacctc aaggtgaagg cgacaggacg
     2281 gttgtagggg tggaggccga ccgaggtcgc gaccactcgt cgccccaggt cggcgggcgc
     2341 tcgccgagga ggccggtctc gcccatggct gccgcccgcc ggccctggac gaccacgtac
     2401 agcacgaagg cgatctcgac ggtggcgccg atgccgaccc ggacgagggt cgacagcccg
     2461 cttcccgtca cgaagccctc gatggtgccg gccacgatga aggcgagggc cagaccgagg
     2521 acgatgacca ccgagcgtcg gccctcttcg gcgacggcgt cgccccgcgt ccggtcgccg
     2581 ggggtgatga gcgcccagcc catctggatc ccggcggcgg cagccagaga gatggcggtg
     2641 agctcgagga ggccgtgggg caggatgagg ccgaagaact tgggggactg gccggcggcc
     2701 acgaacagcc cggccgccac ccccaagttg gccccgttca ggatcagcac gtaggccgcg
     2761 cccaggcaga aggcgatccc ggcggcgaag gccaggatgg acacctggat gttgttgacg
     2821 gtgacctcgg tggcgaactg cgccgccggc tcagacgagt agtacgcctc gaagccctcg
     2881 tcgaggtacg cctcccgcac cgcgtcgggg gcggcggcgt cgagggcctc gtccgacgtc
     2941 ccgatccaag cgccgacc
//
LOCUS       DASTFV010000430         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1584572, whole genome shotgun sequence.
ACCESSION   DASTFV010000430 DASTFV010000000
VERSION     DASTFV010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584572"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..788
                     /locus_tag="VFP02_07950"
     CDS             180..788
                     /locus_tag="VFP02_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9542999.1"
                     /translation="MGAAAFAVSAKLAVDDDARTVESRLFHAVNGLPEWLYPVLWGPM
                     QLGNLVVGAVVALVVALLGRRPNVAVAVVAAVGLKLVVERVLRDKLTADLEGRQRPGT
                     SQTDAILRGDVPVEGHSFPSGHVILVAALAAVLTPLLPRRWATLPWAAVGLVAFGRVY
                     VGAHNPLDVTAGFGIGLAVGSVLDVALGDENVHWSRQAPLRG"
     gene            974..2311
                     /locus_tag="VFP02_07955"
     CDS             974..2311
                     /locus_tag="VFP02_07955"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9543000.1"
                     /translation="MTEADAGRVVIVGGGFAGVGCAQVLGDAGVAVTLIDRNDHHQFQ
                     PLLYQVATSQLAPTDVRRPLRGMFRKAPTVAVKHCDIVAVDPATRTVTSAEGHTFTGD
                     HLVLAMGSQPNFFGLPGAAEHTFPLYSAIDAQRLRDRILQVFEDADLDPSRLDQGALT
                     FVVVGAGPTGVELAGALADLVHEVMPERYHDLDVSRSRIVLVDHGHVVLGAFSDRAHE
                     YAAKVLTHKGVHLRLGTGVDEVQVDGVLLDDGTRIATRCVVWAGGLKATDLSDASDLP
                     RGRGGRVGVDRDLRVEGHEGVYVIGDAANIPGRDGETLPQLGSVALQAGAAAGHSILA
                     AMEGKAPPAFHYHDKGIMAMIGQGAAVAEMGPHRHELHGHIAFSAWLGVHAWLLSGTR
                     ARLDAFMSWGWDSFSHSRSPSTTLDPDAARIDWGDQGDPDEPDPTSNIPAEDP"
BASE COUNT          358 a          876 c          859 g          366 t
ORIGIN      
        1 caggaggccg tcggccccgc cgcgctcaac gaggcgctct ctcgcttcga cccgaagttc
       61 ctgagcgggg cgacgcacac gtcgatcaag gagaacccgc agctgatcgc cgatgtgcgc
      121 agccgcatcg agcagggggc ggcctcctga agcggcacca ggccctcgcc gtcgccggag
      181 tgggcgccgc ggccttcgcc gtctccgcca agctggccgt ggacgacgac gcccggacgg
      241 tggagtcgcg gctgttccat gcggtcaacg gactcccgga atggctctac cccgtcctct
      301 gggggccgat gcagctgggc aacctggtcg tcggtgccgt cgtggccctc gtggtcgcgc
      361 tgctcggcag gcgtccgaac gtggcggtcg cggtggtcgc cgcggtcggg ctcaagttgg
      421 tggtcgagcg ggtgctccgc gacaagctga ccgccgatct ggagggacgg caacggccgg
      481 gcacctcaca gaccgatgcc atcctgcgcg gcgacgtccc cgtcgagggg cacagcttcc
      541 cttcgggcca tgtgatcctc gtggccgcgc tggcggccgt gctcaccccg ttgctgcccc
      601 gccgctgggc caccctgccc tgggcggcag tgggcctggt ggcgttcggg cgggtgtacg
      661 tcggggccca caaccccctc gacgtcacgg ccggcttcgg catcggcctc gccgtcggtt
      721 cggtgctcga cgtcgccctg ggcgacgaga acgtgcactg gtcgcgtcag gccccgttgc
      781 ggggctaggc cccgacctca cgccctcgtc ggggagcagg cccatccggc cgaggcggtg
      841 gagcagggcc atcgagggga agattaacgg gacggcgaga tcgggaggca ggtcgtcccc
      901 agggcgacgt cccacgaggc gaccacggcc gccaccgccg gacaggcagg cgaggcgtgg
      961 ctaactttgc ctgatgacag aggcggacgc gggcagggtc gtgatcgtcg gcggcgggtt
     1021 cgccggtgtc ggctgcgccc aggtcctcgg tgacgccggg gtggccgtca ccctgatcga
     1081 ccgcaacgac caccaccagt tccagcccct gctctaccag gtggcgacgt cgcagctggc
     1141 gccgaccgat gtccgacggc ccctgcgggg gatgttccgc aaggccccca cagtggcggt
     1201 gaagcactgc gacatcgtgg ctgtcgatcc ggcgaccagg acggtaacca gcgccgaggg
     1261 ccacaccttc accggggacc acctcgtgct ggccatgggc tcccagccca acttcttcgg
     1321 gctgccgggc gcggctgagc acacctttcc cctctattcg gccatcgacg cccagcgcct
     1381 ccgggaccgg atcctgcagg tcttcgagga cgcggacctc gatccctccc gcctcgacca
     1441 gggcgccctg acgttcgtcg tcgtgggggc cggaccgacc ggcgtcgagc tcgcgggggc
     1501 gctggccgac ctggtccacg aggtcatgcc ggagcgctac cacgacctgg acgtcagccg
     1561 gagccgcatc gtcctggtcg accatggcca cgtcgtgctc ggcgctttct ccgatcgggc
     1621 ccacgagtac gccgccaagg tcctgaccca caagggcgtg cacttgcggc tggggaccgg
     1681 agtggatgag gtgcaggttg acggcgtgct gctcgacgac ggcacccgca tcgccacccg
     1741 ctgtgtcgtg tgggcgggag gcctgaaggc caccgatctg tccgacgcca gtgatctccc
     1801 ccgcggccgt ggtggccggg tcggcgtcga ccgcgatctt cgggtcgagg gccacgaggg
     1861 cgtctacgtg atcggcgacg ccgccaacat cccgggccgt gacggcgaga ccctgcctca
     1921 gctgggctcg gtcgcactac aggccggcgc agccgcgggc cacagcatcc tggccgcgat
     1981 ggaggggaag gcaccgccgg ccttccacta ccacgacaag gggatcatgg ccatgatcgg
     2041 ccaaggggcg gcggtggccg agatgggccc gcaccgacat gagctccacg gccacatcgc
     2101 cttcagtgcc tggctcgggg tccatgcctg gctgctcagc ggcacccggg cccggctcga
     2161 cgccttcatg tcctggggct gggactcctt ctcccacagc cgcagcccgt cgacgaccct
     2221 cgatcccgac gccgcccgca tcgactgggg tgaccagggc gacccagacg agccagatcc
     2281 cacctccaac atccctgcag aggacccgtg acctgacaga gaatacgcgt ttggaacgtc
     2341 gactgcgcgg tcgatcagct gagcgagatg ggtacgccgt ttctggccaa cgtcgtcggg
     2401 gagtgcaaga acgaaggcaa atcttctcca atagctcgcc ctactcgatg ctgatcacc
//
LOCUS       DASTFV010000431         3377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1608848, whole genome shotgun sequence.
ACCESSION   DASTFV010000431 DASTFV010000000
VERSION     DASTFV010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608848"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFP02_07960"
     CDS             <1..645
                     /locus_tag="VFP02_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9543001.1"
                     /translation="QVRVAVFTGAGDKAFMAGVDLKTFDTDDTEADPDDPGRLPREAL
                     EAVYDCAVPVIAAVNGPALGAGLAYAAVCDLIVAAEHATFGTTEINVGLLGASAHLNL
                     LLGRPKVRELFLTGVPASAAELHRLGAVNRVVPLADLAATAGELAAALAAKSPLALRL
                     AKEALNETEFLPLKEAYRTEQGYTRRLLQHPDSAEARQAFADKREPNWTYQPGD"
     gene            692..1141
                     /locus_tag="VFP02_07965"
     CDS             692..1141
                     /locus_tag="VFP02_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HET9543002.1"
                     /translation="MSESDVLSAPLVVEYPFTRTVGPVQSAFFTGLREGVLVGVRMVD
                     GGVMCPPVEYDPRTSEPLTELVEVGAAGEVTSWAWVSEPRTGNPLSTPFAWALIRLDG
                     ADTAMLHAVDAGSVDAMSTGMRVRVRWADEREGAITDIACFEPETAP"
     gene            1138..1710
                     /locus_tag="VFP02_07970"
     CDS             1138..1710
                     /locus_tag="VFP02_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HET9543003.1"
                     /translation="MSDVTVSPFPEGLWSQPAVATSGIAAIDGVEPVKSIRTPARLDY
                     TYTAGISQSRFLRGMAEGKLLGERCPKCGKVYVPSRGACPVDGIPTEEVVELPSVGTV
                     VSFCVVNVEFTGRGIEIPYASALVVPDGADIAVFGLIQEIPHDQVRMGMRVEAKWKDP
                     ADWETSFENIEWWRPNGEPDAAYESYQEHV"
     gene            1710..2765
                     /locus_tag="VFP02_07975"
     CDS             1710..2765
                     /locus_tag="VFP02_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HET9543004.1"
                     /translation="MRDVAIVGTAQSAHARRIDDRNEVEMLMPVLQELKDGLGVTQAD
                     LDFTCSGSTDYLAGQGFSFVGTLDGVGPYPPISESHVEADGAWALYEAWVKIQTGAAD
                     TALVYSYGKSSPGELPRVLSRQLDPYYYGPLWPDSLSLAALQTRALLDSGKATERDLA
                     EIAVRSRAAAKDNPFATVSGDFDVDEILAEDHLVAPLRKHDCPPLSDGAVAVVLAAEG
                     RARELTENPAWIRGLEHRIDVHALGLRDLTDSPSTRLAAQAAGVSDGALDVAELHAPF
                     TAQEVILREAMGLSDEVDVNPSGGALAANTMMAAGLVRIVEVANRIAAGTAGRGVAHA
                     TSGHCLQQNLVCVLEAS"
     gene            2767..>3377
                     /locus_tag="VFP02_07980"
     CDS             2767..>3377
                     /locus_tag="VFP02_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378494.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HET9543005.1"
                     /translation="MGERVAVVGCGQTRHQATRGDVSIAGLVREAAMRALEDAGMTWA
                     DIDAVVVGKAPDFFEGLMMPELYLAEALGAVGKPMLRVHTAGSVGGSTAIVASSLVSS
                     GVHKRVLTVAFEKQSESEAMWALSLPVPFSQKLVAGAGGFFAPHIRAYMRRSGAPDDT
                     GILVALKDRLNACKNPFAHLHQPHITYESIRDSMMLWDPIRYD"
BASE COUNT          491 a         1246 c         1172 g          468 t
ORIGIN      
        1 caggtccggg tcgccgtgtt caccggcgcg ggcgacaagg ccttcatggc cggggtcgac
       61 ctgaagacct tcgacaccga cgacaccgag gccgatcccg acgacccggg ccgcctgccg
      121 cgggaggccc tcgaggcggt ctacgactgc gccgttcccg tcatcgccgc cgtcaacggc
      181 ccggccctgg gtgcggggct ggcgtacgcc gcggtgtgcg acctgatcgt cgccgccgag
      241 cacgccacgt tcggcaccac cgagatcaac gtcggcctgc tcggggccag cgcccacctg
      301 aacctgctgc tgggtcgacc caaggtgcgg gagctgttcc tcaccggcgt gcccgcatcg
      361 gcagccgagc tgcaccggct gggtgcggtc aaccgggtcg tgccgctcgc cgacctggcg
      421 gcgacggccg gcgagctggc tgcggcgctg gcggccaaga gcccgctggc cctgcgcctg
      481 gccaaggaag ccctcaacga gaccgagttc ctcccattga aggaggccta tcggaccgag
      541 cagggctaca cccgccgcct cctccagcac ccggactcgg ccgaggctcg ccaggccttc
      601 gccgacaagc gcgagcccaa ctggacctac cagcccgggg actgaggccg cagttacgtc
      661 ggaactggaa cgtgttctag tgtcggcggt gatgagcgag agcgacgtcc tgagtgcgcc
      721 cctcgtcgtc gagtacccgt tcacccggac cgtcggcccc gtgcagtcgg ccttcttcac
      781 cgggctgcgc gaaggggtgc tggtcggcgt gcggatggtc gacggcgggg tgatgtgccc
      841 gccggtcgag tacgaccccc ggacctccga gcccctgacg gagctggtcg aggtcggcgc
      901 cgcgggcgag gtgacgagct gggcgtgggt cagcgagccc cggacgggca accccctgtc
      961 cacgccgttc gcctgggccc tgatccgcct cgacggggcc gacacggcga tgctgcacgc
     1021 cgtcgacgcc ggctcggtcg acgccatgag caccggcatg cgggtccggg tccgctgggc
     1081 tgacgagcgc gagggggcca tcaccgacat cgcctgcttc gagccggaga cggcgccatg
     1141 agcgacgtca ccgtcagccc gttcccggag ggcctctggt cccagcccgc cgtggccacc
     1201 tccggcatcg ccgccatcga cggcgtggag ccggtcaagt cgatccgcac gccggcccgc
     1261 ctggactaca cgtacaccgc cggcatcagc cagtcccgct tcctgcgggg catggccgaa
     1321 ggcaagctcc tgggcgagcg gtgccccaag tgcggcaagg tctacgtccc ctcccggggc
     1381 gcctgtcccg tcgacggcat ccccaccgag gaggtcgtcg agctgccgag cgtgggcacg
     1441 gtcgtcagct tctgcgtcgt gaacgtcgag ttcacgggcc ggggcatcga gatcccgtac
     1501 gcctcggccc tggtggtgcc cgacggcgcc gacatcgcgg tcttcggtct catccaggag
     1561 atcccccatg accaggtccg catgggcatg cgggtcgagg ccaagtggaa ggacccggcc
     1621 gactgggaga cgtccttcga gaacatcgag tggtggcgcc ccaacggcga gcccgacgcc
     1681 gcctacgagt cgtaccagga gcacgtctga tgcgcgacgt ggccattgtc ggcaccgcgc
     1741 agtcggcgca cgcccggcgc atcgacgacc gcaacgaggt cgagatgctc atgccggtgc
     1801 tccaggagct gaaggacggc ctcggggtca cccaggccga cctcgacttc acctgctcgg
     1861 gcagtacgga ctacctggcc ggccagggct tcagcttcgt cgggaccctc gacggtgtgg
     1921 ggccgtaccc gccgatcagc gagtcccacg tcgaagccga cggggcctgg gccctctatg
     1981 aggcctgggt caagatccag accggggcgg cggacaccgc gctcgtgtac agctacggca
     2041 agtcctcccc gggcgagctg ccccgggtgc tcagccgcca gctcgacccc tactactacg
     2101 ggcccctgtg gccggactcg ctcagcctgg ccgccctgca gacccgggcc ctgctcgact
     2161 cgggcaaggc cacggagcgg gatctggccg agatcgccgt ccgctcccgg gcggcggcca
     2221 aggacaaccc gttcgccacc gtcagcgggg acttcgacgt cgacgagatc ctggcggagg
     2281 accacctcgt ggccccgctg cgcaagcacg actgcccgcc cctcagcgac ggcgccgtcg
     2341 ccgtcgtcct cgccgccgag ggccgagccc gtgagctgac cgagaacccg gcgtggatcc
     2401 ggggcctcga gcaccgcatc gacgtgcacg ccctcggcct gcgagacctc accgactcgc
     2461 cctccacccg cctcgcggcc caagcggcgg gggtcagcga cggcgcgctc gacgtcgccg
     2521 agctccacgc ccccttcacc gcccaggagg tcatcctccg ggaggcgatg ggcctgagcg
     2581 acgaggtcga cgtcaacccg tcgggcgggg cgctggccgc caacaccatg atggcggccg
     2641 ggctggttcg catcgtcgag gtggccaacc gcatcgccgc gggcacggcc ggtcggggcg
     2701 tggcccacgc cacgtcgggc cactgcctgc aacagaacct cgtttgtgtc ctggaggcga
     2761 gctgagatgg gtgagcgcgt agccgtcgtc ggatgtggcc agacccgcca ccaggccacc
     2821 cggggcgacg tgtccatcgc cggcctcgtc cgggaagcgg ccatgcgggc cctcgaggac
     2881 gccggcatga cctgggccga catcgacgcc gtcgtggtgg gcaaggcccc ggacttcttc
     2941 gaaggcctga tgatgcccga gctgtacctg gccgaagcgc tgggggcggt gggcaagccc
     3001 atgctccggg tgcacaccgc aggctccgtg gggggctcga ccgccatcgt ggccagcagc
     3061 ctcgtgtcgt ccggcgtcca caagcgggtg ctgacggtgg ccttcgagaa gcagtccgag
     3121 tcggaggcca tgtgggccct gtcgctgccc gtgcccttct cccagaagct ggtggccggg
     3181 gcaggaggct tcttcgcgcc ccacatccgg gcctacatgc gccggtcggg cgcccccgac
     3241 gacaccggga tcctggtcgc cctgaaggat cggctcaacg cctgcaagaa ccccttcgcc
     3301 cacctgcacc agccgcacat cacctacgag tcgatccgcg actcgatgat gctctgggac
     3361 ccgatccgct acgacga
//
LOCUS       DASTFV010000432         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1610145, whole genome shotgun sequence.
ACCESSION   DASTFV010000432 DASTFV010000000
VERSION     DASTFV010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610145"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFP02_07985"
     CDS             complement(<1..224)
                     /locus_tag="VFP02_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9543006.1"
                     /translation="MPSGRVLVAAALTTVVAIAALLVLSRDDSGAGDPRPARDGPSQY
                     TVREGDTLTAVADLHGLPVADLMDTLGLTLA"
     gene            430..867
                     /locus_tag="VFP02_07990"
     CDS             430..867
                     /locus_tag="VFP02_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HET9543007.1"
                     /translation="MGTDLHTGVADRLREADQRYTRGRRSLVDVLEEATRPLTIQEIL
                     GRDKALAQSSAYRNLVILEQAGVVHRVVSTDEFARYELAEHLTDHHHHHLICSQCGQV
                     ADFTVPPEVEEALAGALGRAADAAGFVTQDHRLDLIGQCRRCA"
     gene            complement(996..1778)
                     /gene="aztA"
                     /locus_tag="VFP02_07995"
     CDS             complement(996..1778)
                     /gene="aztA"
                     /locus_tag="VFP02_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein AztA"
                     /protein_id="HET9543008.1"
                     /translation="MSHPNENDSRSQADAVTADGMTLAHGQHVALADATFAIPSGRTT
                     AIIGPNGSGKSTLLHAIAGLHPPRRGTIEVIDRAAVAYVPQHLHANEQLPISAREVVT
                     MGRYAHTGPWRRLRRTDRRAVDVAMGRLDVTDLGDRQLRELSGGQRQRVLVAQALAQE
                     SRLLLLDEPLTGLDPPSQAQILAVIDEERAAGRTVVTSTHSLADAATADHLLLLAGRV
                     VAEGSPEEVLTEAILTEAYGTMVVRFGDRSLLVDDSTHHHPN"
     gene            1779..>2036
                     /locus_tag="VFP02_08000"
     CDS             1779..>2036
                     /locus_tag="VFP02_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HET9543009.1"
                     /translation="MNGNHSRPVGEFRQPRCRLLGGVALVALLSSLVGCGASGAGDGR
                     PLVVATHSILGDLATNVLGDTATVQVVMPAGTDPHDFEPSAA"
BASE COUNT          286 a          693 c          772 g          285 t
ORIGIN      
        1 gccagggtca agccgagggt gtccatgagg tcggcgaccg gtaggccgtg caggtccgcc
       61 accgcggtca gcgtgtcgcc ctcccgcacc gtgtactgcg aggggccgtc ccgcgccggt
      121 cggggatcgc cggcgccgga gtcgtcgcgg ctgaggacga gcagggcggc gatggcgacc
      181 accgtcgtca gggcggcggc gaccagcacc cggcccgagg gcacggcgcg gcgcgtcgtc
      241 ggcggtggga ccacgggggg gacgaggggg ccctcgccga ccgacggaga ggtgtcggtc
      301 agagtgatgc tcccaggatg gggtgacgcg acgcgagtgt caacgcgggc gcgggaggaa
      361 cggggtcagg cgggcttccg tcgacgacgt tcctactcat tctcagaagg ccggatagtc
      421 tgggaaccga tgggaacgga cctgcacacc ggcgtggctg atcggctgcg ggaggccgac
      481 cagcgctaca cgcgcggccg gcgatctctg gtcgacgtcc tggaggaagc cacccgcccg
      541 ctcaccatcc aggagatcct cggccgggac aaggccctgg cccagagctc ggcctaccgg
      601 aacctggtca tcctcgagca ggcgggtgtc gtgcaccgcg tggtcagcac cgatgagttc
      661 gcccgctacg agctggccga gcacctgacc gaccaccatc accaccacct gatctgctcc
      721 cagtgcggcc aggtcgccga cttcaccgtg ccccccgagg tcgaggaggc gctggccggc
      781 gctctgggcc gggcggcgga cgcggcgggg ttcgtgaccc aggatcaccg gttggacctc
      841 atcgggcagt gccggagatg cgcctgaacc ggtaccctcc cggccatgtc gaagcggacc
      901 aacaagcgac gtctgcgcgc ccgtcgcaag aaggcgaacc acggccgcaa gccgaacgcc
      961 agtcgcaagc gctagtcggg taacggccac cctggtcagt tggggtggtg atgggtcgag
     1021 tcgtcgacca gcaggctccg gtcaccgaag cggacgacca tcgttccgta ggcctcggtc
     1081 aggatggcct cggtcagcac ctcctcgggg gagccttcgg cgacgacgcg cccggccagc
     1141 aacagcaggt gatccgcggt ggccgcgtcg gccaggctgt gcgtgctggt caccacggtc
     1201 cgtcccgccg cccgctcctc gtcgatgacg gcgaggatct gggcctgcga cggcggatcc
     1261 aggcccgtga gcggctcgtc cagcaggagg agccggctct cctgggccag cgcctgcgcc
     1321 accaacaccc gctggcgctg gccgcccgag agctcgcgga gctggcggtc gccgaggtcg
     1381 gtcacgtcga gccgacccat cgcgacgtcg acggccctgc ggtctgtcct gcgcaagcgg
     1441 cgccacggcc cggtgtgggc gtagcggccc atcgtcacga cctcccgggc cgagatcggg
     1501 agctgctcgt tggcgtggag gtgctggggg acataggcca ccgcggcccg gtcgatgacc
     1561 tcgatggtcc cgcggcgggg cgggtggagg ccggcgatgg cgtggagcag cgtcgacttg
     1621 cccgagccgt tggggccgat gatggccgtg gtccggcccg aggggatggc gaaggtggcg
     1681 tcggccaggg cgacgtgctg gccgtgggcc agcgtcatgc cgtcggcggt gacggcgtcg
     1741 gcttgtgagc gggaatcgtt ctcgttaggg tgcgacatgt gaacgggaac cattctcgcc
     1801 cggtcgggga gttccggcaa ccccgttgcc gccttctcgg cggcgtggcg ctggttgccc
     1861 tgctgtcgtc cctggtcggc tgcggtgcgt ccggcgcagg cgacggccgc ccgttggtcg
     1921 tggccaccca cagcatcctg ggcgacctgg ccacgaacgt cctcggtgac acggcaacgg
     1981 tccaggtggt catgcccgcc ggcaccgacc cgcacgactt cgagccgtcg gcggcc
//
LOCUS       DASTFV010000433         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1612031, whole genome shotgun sequence.
ACCESSION   DASTFV010000433 DASTFV010000000
VERSION     DASTFV010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612031"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..381)
                     /locus_tag="VFP02_08005"
     CDS             complement(103..381)
                     /locus_tag="VFP02_08005"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HET9543010.1"
                     /translation="MSTLDTEFTATLQKSPNEGGWTYVVMPGSADYFGTRGLVKVRGT
                     VDGHPFQSSFMALGDGTHKLPVKAGVRKAIAKEAGDPVTVVLQERLDA"
     gene            513..911
                     /locus_tag="VFP02_08010"
     CDS             513..911
                     /locus_tag="VFP02_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9543011.1"
                     /translation="MSAPLIVALPTADRPTTYLFYGEGLGFEAIGERADDGVPEPLQF
                     VLNDGARLMFIPTGGFGWVTGDHETAPPGTSECLLSLNVESDAEVDDHIEKARAAGAV
                     VLAEPEQKPWGYLGLFADPDTHLWQVIHPS"
     gene            complement(895..1806)
                     /locus_tag="VFP02_08015"
     CDS             complement(895..1806)
                     /locus_tag="VFP02_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9543012.1"
                     /translation="MRLYPHDDLGDPTAIVDELRAQARLAAEAGFAGVMTSEHHGGFA
                     GYSPNPLQLAGWLLEAMPTGWCAPCPLLLPLRPVALVAEEVAWLAARFPGRVAVGVAP
                     GALPLDFEALDVPFDEMMPRFRAALPRLVAMLRGEDLGPLQGDLALQSCAAHPITVIS
                     TALSPPAVRRAASVGAGIVYDGASPVERLGELSAIYDEARGSGPKVLIRRVWLGDPPR
                     EAFDRQQDLYRSYASAAAQQHWRDHGFLCRDDPAELAADLTAAMTAAGATCLNLRVHV
                     PGITPEMAREQVTRLGTDVLPRLSSGG"
     gene            complement(1885..2553)
                     /locus_tag="VFP02_08020"
     CDS             complement(1885..2553)
                     /locus_tag="VFP02_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543013.1"
                     /translation="MARRLVVTGMGAAALAETALIHLGRTYGSTAAERATALAGDDIV
                     PHPKVVTNHAVTIDAPPEAVWPWLVQMGWHRAGWYTAHWVDRLLFPANWPSANRILPS
                     LQHIEVGDVIPDGPPEAECGMVVESFEPEASLVLHSTSHLPMSWRDRARLDWTWTFVL
                     VPVDGVTRTRFLFRSRWTTSPWWLTLGGWLAVVPADFVMARDMLHGVKARAEGSTLES
                     PFIS"
     gene            complement(2588..>2865)
                     /locus_tag="VFP02_08025"
     CDS             complement(2588..>2865)
                     /locus_tag="VFP02_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543014.1"
                     /translation="QWYGPSTRQGDRVHLMCVQRPYDSVTVRGIPTKRVERVIDLATG
                     KELEWTTRCSVLDELFNSDPHGEVRIDVPEAVVDDLATVLALDLSPA"
BASE COUNT          417 a          998 c         1053 g          397 t
ORIGIN      
        1 gcctcggcca gatcctcgcc gccgaacccg tctagccgcc gttctggcgc ccgattgcgg
       61 cgccaggccg ccgcaatcag gcgtcagtag caggagatct ggtcaggcgt cgaggcgctc
      121 ctggaggacg accgtgacgg ggtcgccggc ctccttggcg atggcctttc ggacgccggc
      181 cttcacgggg agcttgtgcg tgccgtcgcc cagggccatg aacgagctct ggaacgggtg
      241 gccgtcgacc gtcccccgga ccttcaccag tccccgggtg ccgaagtagt cggctgaacc
      301 cggcatgacc acgtaggtcc atccgccctc gttggggctc ttctgcaggg tggcggtgaa
      361 ctcggtgtcc agggtgctca tgggatcctc ctgcggtcag gtcaccgatt ggacccgccg
      421 ccgcaccgag aatcatcgcg tagggcgggt tgtgggaaca ccgcctgcca ccggaacgat
      481 gagttcgcgt gctcgctccg gtccaatgac ccatgagcgc accgttgatc gtggccctgc
      541 ccaccgccga ccgtcccacg acctacctgt tctatggcga ggggttgggc ttcgaggcga
      601 tcggggagcg ggcggatgac ggggtccccg agccgctgca gttcgtgctc aacgacgggg
      661 cccggctgat gttcatcccg acgggcggct tcggctgggt gacgggcgat cacgagaccg
      721 ccccgcctgg caccagcgag tgcctcctca gcctgaacgt cgagtccgac gccgaggtcg
      781 acgaccacat cgagaaggcc cgggccgccg gcgccgtcgt cctcgccgag cccgagcaga
      841 agccttgggg ctacctcggc ctgttcgccg atcctgacac ccacctctgg caggtcatcc
      901 acccgagctg aggcggggga ggacgtcggt gccgaggcgg gtgacctgct cccgggccat
      961 ctcgggggtg atgccgggga cgtggacgcg gaggttgagg caggtggcgc cggccgccgt
     1021 catcgccgcc gtcaggtccg cggccagctc cgccgggtcg tcgcggcaga ggaagccatg
     1081 gtcgcgccag tgctgctggg cggccgcgga ggcgtagctg cggtagaggt cctgctggcg
     1141 gtcgaaggcc tcccggggcg ggtcgccgag ccagacccgg cggatcagga ccttggggcc
     1201 cgagccgcgg gcctcgtcgt aaatggcgga cagctcaccc aggcgctcga cgggcgaggc
     1261 gccgtcgtac acgatcccgg cgccgacgct cgccgctcgc cggaccgcgg gtgggctgag
     1321 cgccgtgctg atgacggtga tcgggtgcgc cgcgcacgac tgcagggcca ggtcaccctg
     1381 gagcgggccg aggtcctcgc cccggagcat cgcgaccaga cgggggagag cggcgcggaa
     1441 gcggggcatc atctcgtcga agggcacgtc cagggcctcg aagtcgagcg ggagcgctcc
     1501 cggggccacg cccacggcca cccgacccgg gaaacgggcg gccagccagg ccacctcctc
     1561 ggccacgagg gccaccggtc gcagtggcag caggagcggg cagggggcgc accagccggt
     1621 cggcatcgcc tccagcagcc agcccgccag ctgtagcggg ttgggcgagt acccggcgaa
     1681 gccgccgtgg tgctcgctgg tcatcacccc ggcgaagccg gcctcggccg ccagccgggc
     1741 ttgcgccctc agctcgtcga cgatcgcggt cgggtcaccc aggtcgtcgt gcgggtagag
     1801 ccgcatcgac accgacccct gctccggcac acccacacgg agagcctgac actcctcgcc
     1861 ctcggccctc gaggcgggcg gtgttcagga gatgaagggg ctctcgaggg tgctgccctc
     1921 ggcccgtgcc ttcaccccgt ggagcatgtc ccgggccatg acgaagtcgg cggggacgac
     1981 ggccagccag ccaccgaggg tcagccacca cggcgacgtg gtccaccggg accggaacag
     2041 gaagcgggtg cgggtgacgc cgtcgaccgg tacgagcacg aaggtccacg tccagtcgag
     2101 ccgggcccga tcgcgccaac tcatggggag gtggctggtg gagtgcaaga ccagcgatgc
     2161 ctcaggctcg aaggactcga cgaccatccc gcactccgcc tccgggggcc cgtcggggat
     2221 gacgtcgccg acctcgatgt gctggagcga aggcaagatc cggttggcgc tgggccagtt
     2281 ggcggggaac agcaaccggt cgacccaatg ggccgtgtac cagcccgccc ggtgccagcc
     2341 catctggacc agccacggcc acaccgcctc gggcggggcg tcgatcgtca cggcgtggtt
     2401 cgtgaccacc ttcgggtggg ggacgatgtc gtcaccggcc agggccgtgg cccgttcggc
     2461 ggcggtggat ccgtaggtcc gcccgaggtg gatcagggcc gtttcggcca gggccgcagc
     2521 ccccatcccg gtgacgacca accggcgagc catggacgcc atcgtcgtcg atccgacgaa
     2581 ggggggctca ggcggggctc aggtcgaggg cgaggaccgt cgccaggtcg tcgacgacgg
     2641 cttcggggac gtcgatgcgg acctcgccgt gggggtcgct gttgaagagc tcgtccagca
     2701 ccgagcagcg ggtcgtccac tcgagctcct tgcccgtcgc caggtcgatc acccgctcga
     2761 cccgcttcgt cggtatgccc cgcacggtga ccgagtcgta ggggcgctgg acgcacatca
     2821 ggtgcacccg gtcgccctgc cgggtcgacg ggccgtacca ctgcc
//
LOCUS       DASTFV010000434         4191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1617225, whole genome shotgun sequence.
ACCESSION   DASTFV010000434 DASTFV010000000
VERSION     DASTFV010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617225"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1807
                     /locus_tag="VFP02_08030"
     CDS             <1..1807
                     /locus_tag="VFP02_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543015.1"
                     /translation="ESPDTDTGLLVALPESGMPVNVDGVVADPTEWNRNDGFSPGTAL
                     LTQVAGLDVEASGLPAITDIPDSRAEDSPVVVVDTETGERQPVWAELDSGAEADEDRL
                     LFIRPGVNYLEGHHYVVGLRDLVGADGEALQPSAGFLAYRDNLTTDLPVIEDQRDAME
                     QVFTDLGEAGVAREGLYLAWDFTIASERSLSERLLHIRDDAFADLGAAAPEFTVDDVV
                     RDPEEAGVEYVHGTFQVPLYLIADGAAGTRFDNGADGLPQRNGDFTADFECALPTATE
                     VAVPMALYGHGLLGSRDEVEAGNIADFVREHQVAFCATDWIGMSEDDVGNAASILGQL
                     GEFPTLADRSQQGILNFLFLGRLMRHEDGLISAPEFQSPEGPRLDTSALAFDANSQGG
                     IMGGAATAVAQDWTRAVLGVPGMNYSLLLRRSVDFDTYAAILDPAYPDPVEQNLGLSI
                     VQMLWDRAENDGYAQHLTRDPYPDTPEHQVLLNVAFGDHQVSMWSAEIMARTIGASAH
                     QPALADGRHPDTEPLWGLEAVEAYPFAGSALVYWDSGAEVPPITNSPPEVGPDPHEDP
                     RADPANRDQKYAFLWQNELIDVCHDEPCTARSTES"
     gene            complement(2017..3087)
                     /locus_tag="VFP02_08035"
     CDS             complement(2017..3087)
                     /locus_tag="VFP02_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9543016.1"
                     /translation="MTDPAPTPTLPASDYLDPLVWEAEIEDVIRRGWVPICRLDQIAR
                     SGDRYATTIAGHPVVAVRDGDEVRVLTNVCQHRWSTVAEPGCSQGRNLVCPYHRWSYR
                     LDGRLQAAPLAGAFTLDDVALPEVRHEVWLGFVMVNLDGAAEPLGPSVAGLTEILAPW
                     RVDELVTVASRTYPSGWNWKVMVENWIECYHHLGSHRDTVESRNPARTTRITETDEPW
                     TFMTVDTDPAALFPPEQWSVPMETEDAGHLTIWAVFPFLLGGTEAPFSFWLEVTPHTV
                     DRHHVTWHLLTHPDRAAEWDDGMADVIMDGLDEVHREDMVQCAKVDEGLRSGTLATGR
                     LVELETSIAHFHRWLSDRRRAH"
     gene            complement(3109..4089)
                     /locus_tag="VFP02_08040"
     CDS             complement(3109..4089)
                     /locus_tag="VFP02_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9543017.1"
                     /translation="MAEVPTDDQALDAYSEVVMAVAEALLPSVASLRVMSPTDGTGGR
                     RPRGSGSAVVLSPDGLLVTSAHVVDGGHGGSATFADGRELDFDLVGTDRLTDLAALRV
                     QGGDLVAAELGNADDLRVGQLVVAVGNPLGFGGSVSAGVVSALGRSLTTTDGQHARVV
                     DNVIQTDAALHPGNSGGALADSRGRVVGINTAVVGPGIGQGLGLAVPLDPTTQAVLAS
                     LVRDGRVRRAYLGLAGGGRPLPPRAAAQLERTSGVEITSVMANGPAARAGLTPEAIIV
                     AVDGEAVSGMGDLQRLMSAERIGRAVEITVVHADGHTANHAVTPEELGAS"
BASE COUNT          609 a         1469 c         1525 g          588 t
ORIGIN      
        1 cgagtccccg gacaccgaca cgggactgtt ggtggccctg cccgagtccg gcatgccggt
       61 caacgtcgac ggggtcgtgg ccgaccccac ggagtggaac cgcaacgacg gcttcagccc
      121 gggtacggcc ctgctgaccc aggtggccgg tctcgatgtg gaagcgtcgg gccttccggc
      181 catcaccgac atcccggatt cgcgggccga ggactcgccg gtcgtcgtcg tcgacaccga
      241 gacaggtgaa cgtcagccgg tctgggccga gttggactcc ggcgccgaag ccgacgagga
      301 ccgtctcctc ttcatccggc ccggggtcaa ctacctggag ggccaccact acgtcgtggg
      361 tctgcgcgac ctggtgggcg ctgacggcga ggccctacaa ccgtctgccg gcttcctggc
      421 ctaccgggac aacctgacca cggatctgcc ggtcatcgag gaccagcgcg acgccatgga
      481 gcaggtgttc accgacctgg gtgaggccgg tgtggcccgg gagggcctgt acctggcctg
      541 ggacttcacg atcgccagcg agcggagcct gtccgagcgc ctgctccaca tccgggacga
      601 cgccttcgcc gacctgggtg cggccgcccc cgagttcacc gtggacgacg tcgtccggga
      661 cccggaggag gctggcgtgg agtacgtcca cgggaccttc caggtaccgc tgtacctgat
      721 cgccgacggg gccgctggca cccggttcga caacggcgcc gacggcctgc ctcagcgcaa
      781 cggcgacttc accgccgact tcgagtgcgc cttgcccacc gccaccgagg tggccgtccc
      841 catggcgctc tacggccacg gcctcctcgg cagccgggac gaggtggagg cgggcaacat
      901 cgccgacttc gtgcgcgagc accaggtggc gttctgtgcc accgactgga tcgggatgtc
      961 cgaggacgac gtcggcaatg cggcgtcgat cctgggtcag ctcggggagt tcccgacgct
     1021 ggccgaccgc tcccagcagg ggatcctgaa cttcttgttc ctgggccggt tgatgcggca
     1081 cgaggacggc ctgatcagtg cccccgagtt ccagagcccc gagggtccga ggctcgacac
     1141 gagcgccctg gccttcgacg ccaacagcca gggcgggatc atgggtgggg cggccacggc
     1201 cgtggcccag gactggaccc gcgccgtgct cggcgtaccc ggcatgaact actcgctgct
     1261 cctgcgccgc agcgtcgact tcgacaccta tgccgccatc ctcgaccccg cctacccgga
     1321 cccggtcgag cagaacctgg gcctgtcgat cgtccagatg ctctgggacc gggccgagaa
     1381 cgacggctac gcccagcacc tgacccgcga tccctacccg gacacgcccg agcaccaagt
     1441 gctgttgaac gtggccttcg gcgaccacca ggtgtcgatg tggtcggccg agatcatggc
     1501 tcgcaccatc ggtgcctccg cccaccagcc cgcgctggcc gacggccgcc accccgacac
     1561 ggaaccgctg tggggcctcg aggcggtcga ggcgtacccg ttcgccggct ccgccctcgt
     1621 ctactgggac agcggcgccg aggtgccgcc catcaccaac agcccgcccg aggtcggccc
     1681 cgaccctcac gaggaccccc gagccgaccc cgccaatcgc gaccagaagt acgccttcct
     1741 ctggcagaac gagctcatcg acgtgtgcca cgacgagccc tgcaccgcgc ggtcaacaga
     1801 aagctgacca gggcgtctca gccggctgcc gaaggtgatc gcggccgggt cgctgatttg
     1861 cgcgcatccc cgtagggcag gccccgagag ggccagaaaa cggcagccgc cgggcctgga
     1921 gggaagggtg tccaggcccg acagccacct ggggacctgt ctacccgagg gtcgccgtca
     1981 tgagaactcc tccgaccggg ggagatcgcg attttttcag tgggcgcggc gccggtcgct
     2041 cagccaacgg tggaagtggg cgatcgaggt ctccaactcg accaggcgac cggtggccag
     2101 cgtgccgctg cgcagtccct cgtcgacctt ggcgcactgg accatgtcct ccctgtggac
     2161 ctcgtccagg ccgtccatga tcacgtcggc catcccgtcg tcccactcgg cggcccggtc
     2221 cggatgggtg agcaggtgcc aggtgacgtg gtggcggtcg acggtgtgag gggtcacctc
     2281 cagccagaac gagaacggtg cctcggtgcc ccccaacagg aaggggaaga cggcccagat
     2341 ggtcaggtgg cccgcgtcct cggtctccat cgggacgctc cactgctcgg gtgggaacaa
     2401 ggcggcgggg tcggtgtcga cggtcatgaa ggtccatggc tcgtcggtct cggtgatgcg
     2461 ggtggtccgg gccgggttcc gggactcgac cgtgtcgcgg tgcgagccga ggtggtggta
     2521 gcactcgatc cagttctcga ccatgacctt ccagttccag ccggaggggt aggtccggct
     2581 ggccaccgtc accagctcgt cgacccgcca gggagcgagg atctcggtga ggccggccac
     2641 ggacgggccc agcggctcgg ccgcgccgtc caggttgacc atgacgaagc ccagccagac
     2701 ctcgtggcgg acctcgggca gggccacgtc gtcgagcgtg aaggcgccgg ccagcggggc
     2761 ggcctggagg cgaccgtcga gccggtagga ccagcggtgg tacggacaga ccaggttgcg
     2821 gccctggctg cagccgggct cggccaccgt cgaccaccgg tgctggcaga cgttggtcag
     2881 cacccggacc tcgtcgccgt cccgtacggc gaccaccggg tggccggcga tggtcgtggc
     2941 gtagcggtcg ccgctgcggg cgatctggtc gaggcggcag atcggcaccc aaccacggcg
     3001 gatgacgtcc tcgatctcgg cctcccagac gagggggtcc aggtagtcgg aggccgggag
     3061 ggtcggggtg ggggccgggt ccgtcacggc cgcccacgct agatcggcct acgaggcgcc
     3121 gagctcctcg ggggtcacgg cgtggttcgc cgtgtggccg tcggcgtgga cgacggtgat
     3181 ctcgacggcc cggccgatgc gctcggcgct catgaggcgc tgcaggtcgc ccatgccgct
     3241 caccgcctcg ccgtcgacgg ccacgatgat ggcttcgggt gtgaggccgg cccgggcggc
     3301 gggcccgttg gccatgaccg aggtgatctc gacgccactg gtccgctcca gctgggcggc
     3361 cgctcgcggg gggagcggtc ggccgccgcc ggccaggccg aggtaggccc ggcggacccg
     3421 accgtcgcgg acgaggctgg ccagcaccgc ctgggtggtc gggtcgagcg ggacggcgag
     3481 gccgaggccc tggccgatgc ccggcccgac cacggccgtg ttgatgccga cgacccggcc
     3541 ccggctgtcg gccagggcac cacccgagtt gcccgggtgg agagcggcgt cggtctggat
     3601 gacgttgtcg accacccggg cgtgctgtcc gtcggtggtc gtgagtgacc ggccgagggc
     3661 ggacacgacg ccggcgctga ccgagccgcc gaagccgagg gggttgccca cggcgacgac
     3721 gagttggccg acccggaggt cgtcggcatt gcccagctcg gccgcgacca ggtcaccgcc
     3781 ttggacccgg agggctgcca ggtcggtgag ccggtcggtg ccgacgaggt cgaagtccag
     3841 ttcgcggccg tcggcgaagg tggcgctgcc accgtggccg ccgtcgacca cgtgggccga
     3901 ggtgaccagg aggccgtccg gggaaaggac gaccgccgac ccggagccac gaggtcggcg
     3961 gccgcccgtc ccgtctgtcg ggctcatgac ccggagactg gcgacgctgg gcaggagggc
     4021 ctcggccacg gccatgacca cctcggaata ggcgtcgagc gcctggtcat cggtcggcac
     4081 ctcggccacg gccatggggc taggccgtgc cttcctcggc ggcctcggcc aggtcgaagg
     4141 cgacccggac gtcgagctcc tcggtgcccc gcacgatgcg cagggccaga g
//
LOCUS       DASTFV010000435         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1618343, whole genome shotgun sequence.
ACCESSION   DASTFV010000435 DASTFV010000000
VERSION     DASTFV010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618343"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="VFP02_08045"
     CDS             <1..1136
                     /locus_tag="VFP02_08045"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543018.1"
                     /translation="SVRRSPRRYGTIYVRFGEPVSLRETLGPPVDGTDLDPEERSLAL
                     NKLAFEVATRINHVTPITATALLTMALLAALGRAMTFAQVRTAVRWQLADAQRRGLPL
                     TSSARALETDGGLRSALTALADNEVVVAFDGGPDTVYLLGPDQHLAAAFYRNSIIHHF
                     LESAIAQLALVSAAEPGVEDPLEAFWAAALRLRDLLKFEFFFRDRASFRAALEAEMDR
                     DRPGWAECVAEGPDGIGALHPAIPLTAHILLRSFLEAYSVVALALEQRGSAPVTDAKA
                     FLAACDGLGRQRLLQQRIHSPESVSRPLFATGLQLAENLGLTEDGDDLATRRADFARE
                     MRHLLRQVDAVEALALTELGGVLASRAATADGTTPPPAPTARS"
     gene            1142..>2213
                     /locus_tag="VFP02_08050"
     CDS             1142..>2213
                     /locus_tag="VFP02_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET9543019.1"
                     /translation="MDEPTIEWTRELSPFDHLMYRMDADSRSRTSLLTVDILDEVPDW
                     ERLCTDLERASRVALRLRQRVVAPIVPLTPAQWVVDPDFDLRYHVRRIALPAPGSERQ
                     LLDLAQTLHATPLDQGRPLWEATLVEGLDEAIGGKAALIWKMSHAVTDGVGGMALERM
                     TRSEERDPDWGPMPPLPVPEDLSAPDLTRRGIRGLPVSVLRGAARQVAGAVGAVGRAV
                     SDPAAAARGISGFAGSVQRMAGPAPAEPSPLLRGRSLNRRFESHDVALADLRGAAKAH
                     GASVNDAYIAAVCGALRLYHEGMGLPVDTLPLAIPISLRSGDDPVAGNQWSGVRIAAP
                     VGEPDPVVRMRIVREQVLTARSE"
BASE COUNT          281 a          852 c          777 g          303 t
ORIGIN      
        1 actcggtgcg acgctcgccg cggcgctacg ggacgatcta tgtccgcttc ggtgagccgg
       61 tgtccctccg ggagacgttg ggtcccccgg tcgacggcac tgatctcgac cccgaggagc
      121 gcagcctggc cctgaacaag ctggccttcg aggtggcgac ccgcatcaac cacgtcaccc
      181 cgatcacggc gacggccctg ctgacgatgg cgttgttggc cgccctcggc cgggcgatga
      241 ccttcgccca ggtccgcacc gcggtccgtt ggcagctggc cgacgcccag cggcgaggcc
      301 tgcccctgac gtcgagcgcc cgggcgctgg agaccgacgg tggcctgcgg tccgcgctga
      361 cggccctggc ggacaacgag gtggtcgtgg ccttcgacgg gggcccggac accgtctacc
      421 tgctcgggcc cgaccagcac ctcgccgccg cgttctaccg caactcgatc atccaccact
      481 tcctcgagag cgcgatcgcc cagctggcgc tggtcagcgc cgccgagccg ggtgtggagg
      541 acccgctgga ggcgttctgg gctgcggcgc tccgtcttcg ggacctgctg aagttcgagt
      601 tcttcttccg cgaccgcgcc tcgttccggg ccgccctcga ggccgagatg gaccgggacc
      661 gccccggctg ggccgagtgc gtcgccgagg ggccggacgg gatcggcgcc ctgcaccccg
      721 ccatcccgct gaccgcgcac atcctcctcc ggtccttcct cgaggcgtac agcgtggtcg
      781 ccctcgccct cgagcagcgc gggtcggccc cggtgaccga tgcgaaggcg ttcctggcgg
      841 cctgtgacgg gctcggccgc cagcgccttc tgcagcagcg catccacagc cccgagtcgg
      901 tctcccggcc gctcttcgcc accgggttgc agctggccga gaacctcggc ctcaccgagg
      961 acggcgacga cctggccacc cggcgagccg acttcgcccg ggagatgcgc cacctcctcc
     1021 gccaggtcga cgccgtcgag gccctggcgt tgaccgagct cgggggcgtg ctcgcctccc
     1081 gcgccgccac cgccgacggc accaccccgc cccccgcccc cactgcccgg agttgagcgc
     1141 catggacgag ccgaccatcg agtggacccg agagctgtca ccgttcgacc acctcatgta
     1201 ccggatggac gcggacagcc gctcgcggac gtccctcctc accgtcgaca tcctcgacga
     1261 ggtcccggac tgggaacggt tgtgcacgga cctggaacgg gccagccgag tggcgctccg
     1321 gctccgtcag cgggtcgtgg ccccgatcgt gccgctgacg cccgcccagt gggtcgtgga
     1381 ccccgacttc gacctccgct accacgtgcg ccgcatcgcc ttgccggcgc cgggatccga
     1441 acgccagctc ctcgacctgg cccagaccct gcacgccacc ccgctggacc agggccggcc
     1501 cctctgggag gccaccctcg tcgagggcct ggacgaggcc atcgggggaa aggcggcgct
     1561 gatctggaag atgagccacg ccgtgaccga tggagtcggg ggcatggcgc tcgagcgcat
     1621 gacccgcagc gaggagcggg accccgactg ggggccgatg ccgccactgc ccgttcccga
     1681 ggacctctcc gcccccgacc tgacccgaag aggcatccgc gggttgccgg tctcggtcct
     1741 gcggggggcg gcccggcagg tggccggcgc cgtcggcgcc gtcggtcggg cggtgtcgga
     1801 cccggcggcc gcggcgcgcg gcatctctgg gttcgcgggc tcggtgcaga ggatggccgg
     1861 gccggccccg gccgagcctt cacccctgct ccggggtcgg agcctcaacc ggaggttcga
     1921 gagccacgac gtcgcgctgg ccgacctacg cggtgcggcc aaggcccacg gcgcctcggt
     1981 caacgacgcc tacatcgccg cggtgtgcgg ggccctgcgg ctctaccacg aggggatggg
     2041 cctacccgtc gacaccctgc ccctggcgat accgatcagc ctgcgcagcg gtgacgaccc
     2101 ggtcgcgggc aaccagtggt cgggcgtgcg catcgccgcg ccggtgggcg agcccgaccc
     2161 ggtggtccgg atgcgcatcg tgcgggagca ggtcctcacc gcgcggagcg agc
//
LOCUS       DASTFV010000436         2518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1620458, whole genome shotgun sequence.
ACCESSION   DASTFV010000436 DASTFV010000000
VERSION     DASTFV010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620458"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFP02_08055"
     CDS             complement(<1..281)
                     /locus_tag="VFP02_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9543020.1"
                     /translation="MAEGWDWMRVLTEPCPHCGFDASAVAPDDLAAALRDQAAAWEIL
                     LASSDDARLRVRPADGTWTALEYGCHVRDLLSVMTERVARTLVEDGPEYG"
     gene            complement(284..862)
                     /locus_tag="VFP02_08060"
     CDS             complement(284..862)
                     /locus_tag="VFP02_08060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9543021.1"
                     /translation="MPPDEGLALHDAALVSPGPFLEVGTYCGKSAVYLGAAAEANGTV
                     LFTVDHHRGSEENQAGWEHHEADLVDPDSGLMDTLPHFRRTIFGANLEDAVVAIIGDS
                     TTVAAHWTTPLGFLFIDGGHGPEPAHRDYESWVPHVVVGGTLAIHDVFPDPTDGGRPP
                     FELYRRALESLAFEETGATGSLRVLRRVAEGI"
     gene            complement(913..1404)
                     /locus_tag="VFP02_08065"
     CDS             complement(913..1404)
                     /locus_tag="VFP02_08065"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET9543022.1"
                     /translation="MAKSRAEVLARIREADYRKMLKYSAVSAVAFPFTQVLLLIFTVG
                     LDWSFVKANLVAVTIMAFPAYLMNRYWVWGKRDKNRFMTEVLPFWIITLIGLVLSTIC
                     AHYAEQWFAGSELAVNVANAVGFGLVWVFKFLFLDRIMFGNQMHFPPDEELDAELGSA
                     PAV"
     gene            1484..2356
                     /locus_tag="VFP02_08070"
     CDS             1484..2356
                     /locus_tag="VFP02_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HET9543023.1"
                     /translation="MSQEAVDDLCALLDLEAIEVNIFRGTSPDEDRQRVFGGQVAGQA
                     LVAAARTVDADRPVHSLHAYFLRPGDPTMPILFEVDRIRDGRSFTTRRVVAVQHGKAI
                     FNLQASFHRHEDGLDHQVDLADAMWDGTLPPEPDTLPDWRTRMEPFAERFGADLDRPR
                     PIDTRNVDWSWGQRSEPRPPHQRVWLRADGSLPDDPVLHHCVLTYASDMTLLDTTLLP
                     HGSLAADERIMMASLDHAMWFHRPFRADEWLLYVQDTPSTSRGRGLARGEIYTHGGDL
                     AVSVVQEGLVRLMR"
BASE COUNT          401 a          864 c          906 g          347 t
ORIGIN      
        1 ccgtactcgg ggccgtcttc gaccagcgtc cgggccaccc gctcggtcat gaccgagagc
       61 aggtcccgga cgtggcagcc gtactcgagg gccgtccagg tcccgtccgc gggccggacc
      121 cgcagccggg cgtcatcgga cgaggccagc aggatctccc aagcggcggc ctggtcccgc
      181 agcgccgcag ccaggtcgtc aggggccacg gccgaggcgt cgaacccgca gtgcgggcac
      241 ggctcggtga ggacccgcat ccagtcccaa ccttcggcca tggctagatg ccctcggcca
      301 cgcggcgcag gacccgcaac gagccggtgg ccccggtctc ctcgaaggcg agggactcga
      361 gggcccggcg gtagagctcg aacgggggcc ggcccccgtc ggtcgggtcg gggaagacgt
      421 cgtggatggc caaggtccca cccaccacca cgtggggaac ccagctctcg tagtcccgat
      481 gcgccggctc cgggccgtgg ccaccgtcga tgaacaagaa gccgaggggc gtggtccagt
      541 gggcggccac cgtcgtggag tcgccgatga tcgcgaccac ggcgtcctcc aggttggccc
      601 cgaagatggt gcggcggaag tgaggcagcg tgtccatcag cccgctgtcc gggtcgacca
      661 ggtcggcctc atggtgctcc caaccggcct ggttctcctc cgagccgcgg tggtggtcaa
      721 cggtgaagag aaccgtgccg ttggcctcgg cggccgcgcc caggtacacg gccgacttgc
      781 cgcagtaggt gccgacctcg aggaaggggc cgggcgagac cagcgcggcg tcatggaggg
      841 ccaggccctc atccggcggc atgaagcccc gggcagcctc cgcggcggcc tggaggcgcg
      901 ggtccaaggc ggtcagacgg ctggggcgga gccgagctcg gcgtcgagct cctcgtccgg
      961 cgggaagtgc atctggttgc cgaacatgat ccggtcgagg aagaggaact tgaacaccca
     1021 gacgaggccg aagcccacgg cgttggccac gttgacggcg agctccgaac cggcgaacca
     1081 ctgctccgcg tagtgggcac agatggtcga caggacgagg ccgatgagcg tgatgatcca
     1141 gaagggcagg acttcggtca tgaaccggtt cttgtccctc ttgccccaga cccagtagcg
     1201 gttcatgagg tacgcaggga aggccatgat cgtgacggcc acgaggttgg ccttgacgaa
     1261 cgaccagtcg aggccgacgg tgaagatgag caacaagacc tgcgtgaacg ggaaggccac
     1321 ggcggagacc gctgagtact tcagcatctt gcggtagtcc gcctcgcgga tgcgcgcgag
     1381 gacttccgcg cgggacttcg ccatgaagcc catcgtagcg gtgggcgcgg gagcatcgac
     1441 cagggccatg gccgtcctat cggcaccggt agggtccgcg ttgatgtctc aggaagcggt
     1501 cgacgacctc tgcgcgctgc tcgatctcga ggccatcgaa gtcaacatct tccggggcac
     1561 cagcccggac gaggaccgcc agcgggtgtt cggggggcag gtggcgggcc aggccctcgt
     1621 cgccgcggcc cgcaccgtcg acgccgaccg gccggtgcac tcgctgcacg cctacttcct
     1681 gcgccccggc gacccgacga tgcccatcct gttcgaggtc gaccgcatcc gggacgggcg
     1741 ctcgttcacg acacgacggg tcgtggccgt ccagcacggg aaggccatct tcaacctgca
     1801 ggcgtcgttc caccggcacg aagacggcct cgaccaccag gtggacctgg ccgacgccat
     1861 gtgggacggc acgctgccgc ccgagcccga cacgttgccc gactggcgga cccggatgga
     1921 gccgttcgcc gagcgcttcg gcgccgatct cgaccggccc cggcccatcg acacccgcaa
     1981 tgtcgactgg tcgtggggcc agcgcagcga gccgcggccc ccgcaccagc gggtgtggtt
     2041 gcgagccgac gggagcctgc ccgacgatcc ggtgctgcac cactgtgtcc tgacgtacgc
     2101 gtcggacatg acgctgctgg acaccacgct gctgccccac ggttccctgg ccgcggacga
     2161 gcggatcatg atggccagcc tcgaccacgc catgtggttc caccggccgt tccgggccga
     2221 cgaatggctg ctctacgtcc aggacacccc gtcgaccagc cgtggtcggg gcctggcccg
     2281 gggcgagatc tacacccacg ggggtgatct ggccgtgtcc gtcgtgcagg aggggctggt
     2341 ccggctgatg cgatgaggcg gcggaccgcg ggtgctctcg gtaccgccgt gacgctggtt
     2401 ctcctggccg gtgggtgtgg cgacgacgcc gacgaggagc tgcccagcga cacccgggcg
     2461 cccatcgtcg aggaggccga acccacggac gcgccgacga ccacgctggt gggactcg
//
LOCUS       DASTFV010000437         3625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1622092, whole genome shotgun sequence.
ACCESSION   DASTFV010000437 DASTFV010000000
VERSION     DASTFV010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622092"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..789)
                     /locus_tag="VFP02_08075"
     CDS             complement(25..789)
                     /locus_tag="VFP02_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9543024.1"
                     /translation="MGDVLVTGGTGVLGTRVVARLLAGGHTVRITSRRADSAAPEGVA
                     VHAADLKSGQGLVEAVAGVDTIVHAATALKAKKKVEVGGAGRLLEAIGDARPHLLYVS
                     IVGVDRHPYSYYRAKWAAEQVLEAGEVPLTIQRATQFHDLLHRLLSMTISGLPFGATF
                     QPIDVSEVAGRLVELVDAGPAGRAEDIGGPEVLDIEDLTGQYAEVLGTKPRCFTLPAA
                     GKTLQAFGKGIQTTPDHRYGTITWRQFLEAKAAADA"
     gene            821..2005
                     /locus_tag="VFP02_08080"
     CDS             821..2005
                     /locus_tag="VFP02_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543025.1"
                     /translation="MDDLTRADLCGRTERWFVQRGLPHFIDEYRATTDVWTRAAPFLT
                     LVFLAELSFSLDEDREGLGELLPLLGGLAIFVVGVMAVNRLRGRRPLQRPDRVGTPEL
                     SLFVIAPALLPALFSKDHLTDSLGAVLLNVVILALTFVVTSYGLVPMTRWALGQMFRQ
                     LANVADLMVRSLPLLLLFATFLFLNTEIWQVAAELEAPFLLMVLGMLFGAGIIFLVLR
                     QRREVELVSHFRSWPEALECSGSSPVSVVAVDGLVEPPDPEPLGRRARINIALVLVFS
                     QGVQIILVATVIGLFYVGFGLVAVRETTMASWVGAPLDVLGEITVFGHPVVLTWDLIR
                     VAGLVAAFSALQFTFSALTDQTYREEFFEGMLSEMRESLATRAVYLEVLVGAHRTEGV
                     AD"
     gene            2016..2744
                     /locus_tag="VFP02_08085"
     CDS             2016..2744
                     /locus_tag="VFP02_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543026.1"
                     /translation="MRRRLGLLFVVALAATAVVGLTTPAGAGPVPCVDVKGRVTDAAT
                     GLPLSETTSVEIGGVSVKYEDGIGTNAASRWSTCLVPGTYKIKFVADSYRPEWYHDQP
                     NVTLATVITVAPPGPVIVNEALVPRGRVIAGRVTNASGVPKFGSVGIWRRTSTGWRSI
                     DGIGNDGATGWYSFVVPGPGRYRVSAGVDHHKARFATSATHLSAGRIVTIGVPTTFVN
                     DVHVRVPYCPGPTDDICVPAGFFT"
     gene            complement(2751..3542)
                     /locus_tag="VFP02_08090"
     CDS             complement(2751..3542)
                     /locus_tag="VFP02_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9543027.1"
                     /translation="MLDDVTLARLAHLNYLEFGRQSTLWSDRGQVRDADGVTLFASGT
                     DFPFVFNGVFRHDPAADPAQVLARADAFFDQWSRGYTLVTGPGDGDLAAHAEAEGLWA
                     FADAPEMVCRERLADLDPPEGVDLRRATDEAGIADFVAVGSAAYPSLGMPATAMGEAI
                     DRYDRVLEPHVAVVVAYDGDAPVAAAQAMLSHDIAGVYWVGTLEAARGKGLGEAVCRW
                     VTNWAFDQGAAAQSLQASTMGEPIYARMGYETIHRYQSWFRSSDR"
BASE COUNT          481 a         1323 c         1252 g          569 t
ORIGIN      
        1 ctggaggcca cctacgactg acggtcaggc gtcagcggcg gccttcgcct cgaggaactg
       61 gcgccaggtg atggtgccgt acctgtgatc gggggtggtc tggatgccct tgccgaaggc
      121 ctgcagggtc ttccccgcgg ccgggagggt gaaacaacgg ggcttggtcc ccagcacctc
      181 ggcgtactgg ccggtcaggt cctcgatgtc cagcacctcg ggcccaccga tgtcctcggc
      241 ccgccccgcc gggccggcgt cgaccagctc gaccaagcgg cccgccacct cgctcacgtc
      301 gatgggctgg aaggtggcgc cgaagggcag cccggagatc gtcatgctca agagccggtg
      361 cagcaggtcg tggaactggg tcgcccgctg gatggtgagc ggcacctcgc ccgcctcgag
      421 gacctgctcg gccgcccact tggcccggta gtagctgtag gggtgacggt ccacgcccac
      481 gatcgagacg tagagcaggt ggggtcgggc gtcaccgatg gcctccagca gtcgcccggc
      541 gccaccgact tcgaccttct tcttggcctt cagcgccgtg gccgcgtgca cgatggtgtc
      601 gaccccggcc acagcctcga ccagtccctg gcccgacttc agatcggcgg cgtggacggc
      661 caccccctcc ggcgcggccg agtcggcacg gcgtgacgtg atccgcacgg tgtggccgcc
      721 ggcgagcagg cgcgccacga cccgggtccc gagcacgccc gtcccgccgg tgaccaagac
      781 atctcccatg gctcccaacc tacgggccag actctggcgg atggatgacc tcacgcgggc
      841 cgacctctgt gggcggaccg agcggtggtt cgtccagcgg ggcctgcccc acttcatcga
      901 cgagtaccgg gccaccaccg acgtctggac ccgggcggcc cccttcctca ccctcgtctt
      961 cctggccgag ctctccttca gcctcgacga ggaccgggag ggcctgggtg agctcctgcc
     1021 actcctcggc ggcctggcca tcttcgtggt cggcgtgatg gcggtgaacc ggctgcgggg
     1081 ccgtcgcccc ctgcagcgcc cggaccgggt cggcaccccc gagctgagcc tcttcgtcat
     1141 cgcccccgcg ctcctgcccg ccctcttcag caaggaccac ctgaccgact ccctgggcgc
     1201 cgtcctgctc aacgtggtga tcctggccct gaccttcgtc gtgaccagct acggcctcgt
     1261 gcccatgacc cgttgggccc tcggccagat gttccgccag ctggccaacg tggccgacct
     1321 gatggtccgc tcgctgcccc tgctcctgct cttcgccacc ttcctgttcc tcaacaccga
     1381 gatctggcag gtggcggcgg agctggaggc cccgttcctg ctcatggtcc tgggcatgct
     1441 cttcggcgcc gggatcatct tcctcgtgct ccgccagcgg cgcgaggtgg agctcgtatc
     1501 gcacttccgg agctggcccg aggccctcga gtgctcgggg tcctcgccgg tctcggtggt
     1561 ggcggtcgac ggcctggtcg agccgcccga ccccgagccg ctcggccgcc gggctcgcat
     1621 caacatcgcc ctcgtcctgg tcttcagcca gggcgtgcag atcatcctgg tcgcgaccgt
     1681 gatcggcctc ttctacgtgg gcttcggcct ggtggccgtg cgggagacca cgatggcctc
     1741 ctgggtcggc gccccgctgg acgtactcgg cgagatcacg gtcttcggcc acccggtggt
     1801 cctgacctgg gacctgatcc gggtggcggg cctggtcgcc gccttctccg ccctccagtt
     1861 caccttctcg gccctgaccg accagaccta ccgcgaggag ttcttcgagg gaatgctctc
     1921 ggagatgcgg gagtcgctgg ccacgcgggc ggtgtacctc gaggtcctgg tcggtgccca
     1981 ccgaaccgaa ggggtcgcgg actagcgtcc cgccgatgcg acgtcgtctc ggccttctgt
     2041 tcgttgtcgc cctcgcggcc accgctgtgg tcgggctcac gacgccggcg ggcgccgggc
     2101 cggtgccctg tgtcgatgtc aagggtcggg tgaccgacgc cgccaccggc ctgccgctgt
     2161 cggagacgac gagcgtggag atcggcgggg tgtcggtcaa gtacgaggac ggcatcggca
     2221 ccaacgccgc cagccggtgg agcacgtgcc tggtccccgg gacctacaag atcaagttcg
     2281 tggccgacag ctatcggccc gagtggtacc acgaccagcc caacgtcacg ctggccaccg
     2341 tgatcaccgt ggccccccca gggccggtca tcgtcaacga ggccctggtg ccccggggtc
     2401 gcgtcatcgc cggccgggtg acgaacgcga gcggggtgcc caagttcggc agtgtcggga
     2461 tctggcgccg gaccagcacg gggtggcgct cgatcgacgg catcggcaac gacggggcca
     2521 ccggttggta ctccttcgtg gtgcccgggc ccgggcgcta ccgggtctcg gcgggcgtgg
     2581 accaccacaa ggcccggttc gccaccagtg ccacgcacct gagcgcgggc cgcatcgtca
     2641 ccatcggggt gccgacgacg ttcgtcaacg acgtgcacgt acgggtgccg tactgccccg
     2701 gtcccaccga cgacatctgc gtgccggcgg gcttcttcac ctgagcccca tcaccggtcc
     2761 gaggaccgga accagctctg gtagcggtgg atggtctcgt agcccatgcg ggcgtagatc
     2821 ggttccccca tcgtcgaggc ctgcaggctc tgggccgccg ccccctgatc gaaggcccag
     2881 ttggtgaccc agcggcagac cgcctcaccc aggcccttgc cccgggcggc ctccagggtg
     2941 cccacccagt agacgccggc gatgtcgtgg ctgagcatcg cctgggcggc ggcgaccggc
     3001 gcatcgccgt cgtaggccac cacgaccgcc acgtgaggct cgaggacccg gtcgtagcgg
     3061 tcgatggcct cgcccatggc ggtggcgggc atgcccaggg acgggtaggc ggcggagccg
     3121 acggccacga agtcggcgat cccggcctcg tcggtcgccc gacgcaggtc gacgccctcg
     3181 ggcgggtcga ggtcggccag ccgctcccgg cagaccatct ccggcgcgtc cgcgaacgcc
     3241 cagagcccct cggcctcggc gtgggccgca aggtccccgt cgccgggccc ggtgaccagg
     3301 gtgtagcccc gcgaccactg atcgaagaag gcgtcggccc gggccaacac ctgggccggg
     3361 tccgccgcgg ggtcgtgacg gaagaccccg ttgaacacga acgggaagtc ggtgccgctg
     3421 gcgaacagcg tcaccccgtc ggcatcccgg acctggcccc ggtccgacca gagcgtgctc
     3481 tgccgtccga actccaggta gttcaggtgg gccagccggg ccagggtcac gtcgtcgagc
     3541 accctcagat catggcgggt ggccgtccga gtggcggagc gaacaggtgt tcgctacggt
     3601 cggaccatgg ggtggaggaa ccctc
//
LOCUS       DASTFV010000438         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1631609, whole genome shotgun sequence.
ACCESSION   DASTFV010000438 DASTFV010000000
VERSION     DASTFV010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631609"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFP02_08095"
     CDS             complement(<1..378)
                     /locus_tag="VFP02_08095"
                     /inference="COORDINATES: protein motif:HMM:NF014009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HET9543028.1"
                     /translation="MDTTVLEITTGTRPVHDLTADAARFVSGRGDGLLHVFAPHATVG
                     LALMETGAGSDDDLVDLLEQHLVPRDDRYRHAHGSAGHGADHVLPALLSPSVSIPVLD
                     GRLALGTWQSLLLVDTNRDNPRRE"
     gene            405..2042
                     /locus_tag="VFP02_08100"
     CDS             405..2042
                     /locus_tag="VFP02_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543029.1"
                     /translation="MSRRIDVELTSDRGDGTLTWRAAGAKAPKGVLDATLLPTGVKVG
                     DTVKADADYDMEGVTIVAVMAPTEKKKSPDVIEFIGAPPPPPRDYTKEPERRERKPRR
                     GDRDGRGGPGGRGGPGGRGGAGGSGGPSGPGGGRERKPHDRPERPRFERPEKPKLPPK
                     PKAPRLRAGRKHRQALLAELPEEQRPVAEQVLKGGIPAVRAAIDEQNAAAKEAGNPLV
                     NADALLAMAETIVPKARVAEWQDRAEAAIEQIDTVDLRDLRSVVTKAESAAKDEASRE
                     LAERLRTGLVVRLEKEQAAWLGEIAELLADDRVVRALNVSSRPPKAGTPLPRDLAERL
                     TAAAAAALTDQIGSKRWSTVLEALALSPVHAQVEPASVPASPNEELIAAVTRVSMQVP
                     QIAAKFGIEPKPAPRSRGSRSRPRTGGPKPGAPPTPAREKAPRIPPPPPIADTPVGAS
                     APAPAEPAAESEAPVAEAEAAEVVAEVVEAPVAEAEAPEVVAEVVEAPAAEAPVQPEA
                     EAAPEVEAVTVPAESEAPPEAAAAESVAPEDTTPPSA"
     gene            complement(2241..>2603)
                     /locus_tag="VFP02_08105"
     CDS             complement(2241..>2603)
                     /locus_tag="VFP02_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HET9543030.1"
                     /translation="YDPSVSDPFPGIDDLLASLPRWGLCSNKHPDSGASELARLGWHP
                     TATAFALGAPKSLLPVLTDLGVSGADVLYVGDTDHDRECAVEVGATFALAGWNRRAQP
                     VPGDVVLNAPAQVLDLLA"
BASE COUNT          377 a          928 c          991 g          307 t
ORIGIN      
        1 ctcgcggcgg gggttgtccc ggttggtgtc gaccaggagg agcgactgcc aggtgccgag
       61 ggccagccgt ccgtcgagga cgggaatgct caccgatggc gacagcaggg ccgggagcac
      121 gtggtcggcg ccgtggccgg cgctgccgtg agcgtggcgg taacggtcgt cgcggggtac
      181 gaggtgctgc tcgagcaggt ccacgaggtc gtcgtcggat cccgcgcccg tctccatcag
      241 ggccaggccg accgtggcgt ggggggcgaa cacgtggagg aggccgtcgc cccgaccgct
      301 cacgaatcgg gcggcgtcgg ccgtcaagtc gtggacagga cgggtgccgg tggtgatttc
      361 caggacggtc gtgtccacgg gccgacgata cggtggttcg ccgtatgtcc agacggatcg
      421 atgtcgagct caccagtgac cgcggggacg ggacgctcac gtggcgtgcc gcgggcgcca
      481 aggcccccaa gggcgtgctc gacgccaccc tgctccccac gggggtgaag gtcggcgaca
      541 ccgtcaaggc cgatgccgac tacgacatgg agggcgtcac catcgtcgcc gtgatggcgc
      601 cgacggagaa gaagaagtcg cccgacgtca tcgagttcat cggcgcaccg cccccacccc
      661 cccgggacta cacgaaggag cccgagcgtc gggagcgcaa gccccgtcgg ggcgatcgag
      721 acggccgcgg tgggccgggt ggtcgcggtg gccccggtgg ccgtggcggt gcgggtgggt
      781 ccggtggacc gagtggaccc ggtggtggcc gggagcgcaa gccccacgac cgtccggagc
      841 gcccccgctt cgagcggccc gagaagccca agctgccgcc gaagcccaag gcgcctcgcc
      901 tccgggccgg gcgcaagcac cgccaggccc tcctggccga gctgcccgag gagcagcgcc
      961 cggtcgccga gcaggtcctg aagggcggca tccccgccgt gcgggccgcc atcgacgagc
     1021 agaacgccgc ggccaaggag gcgggcaacc cgctggtgaa cgccgacgcc ctcctggcca
     1081 tggccgagac gatcgtcccc aaggcccgcg tcgccgaatg gcaggatcgg gccgaggccg
     1141 ccatcgagca gatcgacacc gtcgacctgc gggacctgcg ctccgtggtc acgaaggccg
     1201 agagtgccgc caaggacgag gcctcccgcg agctcgccga acggctgcgc accggcctgg
     1261 ttgtccgcct cgagaaggag caggccgctt ggttgggcga gatcgccgaa ctgctggccg
     1321 atgaccgcgt ggtgcgggcg ctgaacgtca gctcgcgccc gccgaaggcc ggcacgcccc
     1381 tgcctcgcga tctggccgag cgcctcacgg cggcggcggc cgccgccctg accgaccaga
     1441 tcggctccaa gcgctggagc acggtgctcg aggcccttgc cctctcgcct gtgcacgccc
     1501 aggtggagcc ggcctccgtg cccgcctcgc ccaacgagga gctgatcgct gccgtcaccc
     1561 gggtgtcgat gcaggtcccc cagatcgcgg ccaagttcgg catcgagccc aagcccgcgc
     1621 cccgctcccg cggcagtcgc tcccggccgc gaacgggcgg cccgaagccg ggcgccccgc
     1681 cgaccccggc gcgggaaaag gctccccgga tcccgccgcc cccgcccatc gccgacaccc
     1741 ccgtcggggc cagcgcaccg gctcccgcgg agcctgcggc ggagtccgag gctcctgtgg
     1801 ccgaggctga ggcggcggag gtggtggctg aggtggtcga ggctcctgtg gccgaggctg
     1861 aggcgccgga ggtggtcgct gaggtggtcg aggcccctgc cgccgaggct ccggtgcagc
     1921 ccgaggccga ggctgcgccc gaggtcgagg ccgtcacggt gcctgctgag agcgaagctc
     1981 cgcccgaagc cgcggcggcc gagtccgttg cgcccgagga caccacaccc ccctcggcgt
     2041 aggccggtcg tcctggggcc gaacgccgtt cggctgggcc aaccgaagca accacgaggg
     2101 cggaccctgt cgtaggtgca gctccgcccc gtcagatcgg gtaccgggcg aactggcgcc
     2161 tgatcctggg cctatgacac cgcaatcggg cgccagaacg ggggggggct cggtccagcc
     2221 cgacggcggg gcggtaggtg tcaggccagg aggtcgagca cctgggcggg agcgttcagc
     2281 acgacgtctc cgggcacggg ctgggcccga cggttccagc cggccagcgc gaacgtggcg
     2341 cccacctcca ccgcgcactc gcggtcgtgg tcggtgtcgc cgacgtagag gacgtccgcg
     2401 ccgctgacgc cgaggtcggt cagcaccggc aggagggact tcggtgcccc cagggcgaag
     2461 gccgtcgccg tcggatgcca gccgaggcga gccagctccg aggcgccgga gtcggggtgc
     2521 ttgttggagc agagccccca acggggcaac gacgccaaca ggtcgtcgat cccgggaaac
     2581 gggtccgaca cgctcgggtc gta
//
LOCUS       DASTFV010000439         2507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1641176, whole genome shotgun sequence.
ACCESSION   DASTFV010000439 DASTFV010000000
VERSION     DASTFV010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641176"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VFP02_08110"
     CDS             complement(<1..507)
                     /locus_tag="VFP02_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET9543031.1"
                     /translation="MEDVAIIGVGLHPFGRFGDKPALEMGAEAVRAACADAAVPWKDV
                     QFAFGGSAELDTPDVVVNRLGLTGIPFMGVYNGCATAATALQLTADIIRSGQHEIGVA
                     VGMDKHLPGALTADPVNYGAPSWYGDLGYFLTTKFFGMKINRYMHDHGISHETLAKVA
                     AKNFRNGGL"
     gene            complement(517..933)
                     /locus_tag="VFP02_08115"
     CDS             complement(517..933)
                     /locus_tag="VFP02_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HET9543032.1"
                     /translation="MPTVAYPPDVFTWPADEPQLIAGRCADCQAIAFPVATSCPRCGS
                     TAVDEHLLPRRGTLWTWTTQDFLPKEPYASGETDETFRPYGVGLVQLGDEVRVEARLT
                     EADPAKLDFGMEVELVVVPFRDDGDTTTVTFAFKPV"
     gene            complement(944..2392)
                     /locus_tag="VFP02_08120"
     CDS             complement(944..2392)
                     /locus_tag="VFP02_08120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HET9543033.1"
                     /translation="MSDPRRLPGTIAEIEAGPAGPEIGVFFDLDGTLIAGYSARYLAE
                     DRIRRREISGSEFVRTFALMVGSPFNRVTFEDIMQIGAEAWRGRAHEDLEEMGLRVFE
                     KKIAGRIYPEMRDVVQAHQKRGHTVALTSSATSYQAEPVADFLGIDEVICNRYEVEDG
                     LLTGKVEQPVLWGPGKADAVQAFAAKRGIDLADSYFYADGDEDAALMYLVGHPRPTNP
                     APRLARVAKMRGWPVLSFTSRGAGGLGSLVRSAASLGSMLPIAGAGLGIGLVRRDKRA
                     AMNFVCGTWIDTTFALNGVHLNITGKENAWSQRPAVFIFNHRNNFDSLIAASIVRSDF
                     TGVAKAEMAKDPITRTAGQLMDVAFIDRSDPAAAVASLKPLEELARKGLSVLVAPEGT
                     RLDTRQVGPFKKGAFRIAMASGLPIVPIVVRNAEVIAGHDSSMLNPGTVDVTILPPIP
                     VTDWKLEDLDQHIADVRDIYLQTLADWPGSQP"
BASE COUNT          356 a          827 c          925 g          399 t
ORIGIN      
        1 caggccaccg ttccggaagt tcttcgccgc gaccttggcc agcgtctcgt gcgagatgcc
       61 gtggtcgtgc atgtaccggt tgatcttcat cccgaagaac ttggtcgtca ggaagtaccc
      121 gaggtcgccg taccacgacg gcgcgccgta gttgacgggg tcggcggtca gggccccggg
      181 caggtgcttg tccatgccga cggcgacgcc gatctcgtgc tgaccggacc ggatgatgtc
      241 ggcggtgagc tggagggccg tggccgccgt ggcgcagccg ttgtagaccc ccatgaaggg
      301 gatgccggtc agcccgagcc ggttgaccac gacatcgggc gtgtccagct cggcgctgcc
      361 gccgaaggcg aactgcacgt ccttccacgg cacggccgcg tcggcgcagg cggcccgcac
      421 ggcctcggcc cccatttcga gggcgggctt gtcgccgaag cgaccgaagg ggtggaggcc
      481 caccccgatg atggcgacgt cttccatggc ggtctcctag acgggcttga aggcgaacgt
      541 gacggtggtg gtgtcgccgt cgtcccggaa gggcacgacg acgagctcga cctccatgcc
      601 gaagtcgagc ttggccggat cggcctcggt caggcgggcc tcgacccgca cctcgtcacc
      661 cagctgcacg agaccgaccc cgtagggccg gaacgtctcg tctgtctccc cgctggcgta
      721 cggctccttg ggcaggaagt cctgggtggt ccaggtccac agcgtccccc ggcgcgggag
      781 caggtgctcg tcgaccgcgg tcgacccgca gcgcgggcag gaggtggcca cggggaaggc
      841 gatggcctga cagtcggcgc agcgcccggc gatgagttgg ggctcgtcgg ccggccaggt
      901 gaagacgtcc ggggggtagg caacggtcgg catcgggttt ctcctaaggc tgagaacccg
      961 gccagtcggc cagggtctgc aggtagatgt cgcggacgtc ggcgatgtgc tggtcgaggt
     1021 cctccagctt ccagtccgtc accgggatcg gcggcaggat ggtcacgtcg acggtgcccg
     1081 gattgagcat ggaggagtcg tggccggcga tgacctctgc gttgcggacg acgatgggca
     1141 cgatcggcag gcccgacgcc atggcgatgc ggaacgcccc cttcttgaac ggccccacct
     1201 gccgggtgtc caggcgggtg ccctcggggg cgaccagcac cgagaggccc ttccgggcca
     1261 gctcctcgag gggcttgagg gaggcgacgg cggcggccgg gtcggagcgg tcgatgaagg
     1321 ccacgtccat gagctggccg gcggtcctgg tgatcgggtc cttcgccatc tcggccttgg
     1381 ccacgcccgt gaagtcactc ctcacgatgc tggccgcgat gagggagtcg aagttgttgc
     1441 ggtggttgaa gatgaacacc gccggtcgct gggaccaggc gttctccttg ccggtgatgt
     1501 tcagatggac gccgttgagg gcgaaggtgg tgtcgatcca ggtcccgcac acgaagttca
     1561 tggcggcgcg cttgtctcgg cgcaccagcc cgatgccgag cccggccccg gcgatcggga
     1621 gcatggagcc caggctcgcc gccgagcgca ccagcgatcc gagcccgccg gctccccggc
     1681 tggtgaagct cagcacgggc cagccccgca tcttggccac gcgggcgagg cgcggcgccg
     1741 ggttggtcgg ccgcgggtgg ccgaccaggt acatcagggc ggcgtcctcg tcaccatcgg
     1801 cgtagaagta gctgtcggcc aggtcgatgc cccgcttggc ggcgaaggcc tgcacggcgt
     1861 cggccttgcc cgggccccag agcaccggct gctcgacctt gccggtgagg aggccgtcct
     1921 cgacctcgta ccggttgcag atcacctcgt cgatgcccag gaagtcggcc accggttcgg
     1981 cctggtagct cgtggccgac gaggtgaggg ccacggtgtg cccccgcttc tggtgggcct
     2041 gaacgacgtc tcgcatctcg gggtagatcc ggccggcgat cttcttctcg aagacccgga
     2101 gccccatctc ctcgaggtcc tcgtgggccc gcccccgcca ggcctcggcc ccgatctgca
     2161 tgatgtcctc gaacgtcact cggttgaacg ggctgcccac catcagggcg aaggtgcgga
     2221 cgaactccga gccgctgatc tcgcggcgcc ggatccggtc ctcggccagg tagcgggcgg
     2281 agtacccggc gatgagcgtg ccgtcgaggt cgaagaagac gccgatctcg gggccggcgg
     2341 gtccggcctc gatctcggcg atcgtcccgg ggaggcgtcg cgggtcgctc acgccgacct
     2401 cggcttccgt cgggaggcct tggccggttc gaccggcgcg agggcgatga cctcgtcgaa
     2461 gccctgtagg aggctgtcgg cgaacagatc ggcgttggtc accgagg
//
LOCUS       DASTFV010000440         2807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1646205, whole genome shotgun sequence.
ACCESSION   DASTFV010000440 DASTFV010000000
VERSION     DASTFV010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646205"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1332)
                     /locus_tag="VFP02_08125"
     CDS             complement(103..1332)
                     /locus_tag="VFP02_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET9543034.1"
                     /translation="MRRPLVPLWRARTGLWSSDRAQTLTALAVNSTTSLVAGVFLGAL
                     TGTFERLPGLLVLVPAAIGLRGNVFSALGSRLSTSVHAGTFSLSRRRGTVVVENVIGA
                     LLLTSGVSLVLAGGAWLLAGAVLATPPIPLLQLATVSVAGGLLASTVVLAVTIGLAVL
                     AAERGWDLDNLVAPVVSTMGDVLTLPALWLATLLLDHGASSTVVGWFLVAVAAAGSAY
                     GVATRRPLLASVCRQSWPILVAAGGLSLLAGLVIEHRLAAFTEAPALLILVPAFVSSA
                     GALGGILAARVATGLHLGTRLGGLAPDRETRDDARTIALLALPVMVYNATGARAVAGL
                     VGPAAPPLGDLLVTTLLAGTGAVAFAIVVAYLSSVAAVRLRLDPDTYGIPVVTSSVDL
                     VGAVLLTGTAVALGVVA"
     gene            1459..1863
                     /locus_tag="VFP02_08130"
     CDS             1459..1863
                     /locus_tag="VFP02_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HET9543035.1"
                     /translation="MSGNIPFVDYLALEPEPHLVANECTSCGARFFDRRNACASCFGT
                     EFRKADIATEGELRSYTIVAFAAPGIPVPFVAGIIDCDGTSVRGNVINVEPDAEHVQL
                     GMKVKLTTVPVGEDTAGTEAVNYAFEPLEGAA"
     gene            1863..>2807
                     /locus_tag="VFP02_08135"
     CDS             1863..>2807
                     /locus_tag="VFP02_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET9543036.1"
                     /translation="MASDDIWILGIHMTKFGKRPDDDVIDLAAEASLAALKDAGVTMA
                     DIGVLAAGNLMGASAPTGQMLQKQIGQTGIPVFNVSNACATGATALRTAIMAIKAGET
                     DMGLAVGVEKLSGAGLLSGGSRKADADEWTPSGRYGAVAGVDGRIGTEIMPGVFAQVG
                     MEYSHKYGGADFELFARISEKNHSHSTLNPLAAYTKKMTLEEIMGDVMIAYPNTRPMC
                     SANCDGAAAAVVVSDAKLKTLDPDQQRRAVKVSASVLTSDPWEEACQILPDVNTLTRR
                     AATQAYEQAGVAPEDLDLVELHDCFATAELVHYDNLMLC"
BASE COUNT          470 a         1000 c         1011 g          326 t
ORIGIN      
        1 atctacgacg cgtggtccca gcccgccgac ccggccccga cctccacgac cacgacggtg
       61 gtcccgcccg agaccgaggc cccgcctgtc accctgggct gatcaggcca ccacaccgag
      121 cgccacggcc gtgccggtca gcaggaccgc gcccaccagg tcgaccgacg aggtgacgac
      181 cgggatgccg taggtgtcgg ggtccagacg gagacggacc gcggcgacgc tgctgaggta
      241 ggccacgacg atggcgaagg ccacggcccc ggtgccggcg agcagggtgg tcaccagcag
      301 gtcccccagg ggtggcgcgg cgggtccgac cagccccgcc acggccctgg cgccggtggc
      361 gttgtagacc atgacgggca gagccaggag ggcgatggtc cgggcatcgt cccgggtctc
      421 ccggtccgga gccaggccgc ccagccgggt cccgaggtgc aggccggtgg ccacccgggc
      481 cgccaggatc ccgcccaggg ccccggcgct gctcacgaag gcgggcacca ggatgaggag
      541 agcgggagct tcggtgaacg cggccaggcg gtgctcgatg accagcccgg ccagcaggct
      601 caggccaccg gccgcgacca ggatcggcca ggactggcgg cacaccgaag ccaggagcgg
      661 acgtcgagtg gcgacgccgt aggcgctgcc ggccgcggcc acggcgacga ggaaccagcc
      721 caccaccgtg ctggaggcgc cgtggtcgag caggagcgtg gccagccaga gagcgggaag
      781 cgtgaggacg tcgcccatcg tggacacgac aggggcgacg aggttgtcga ggtcccaccc
      841 ccgctcggcg gccaagaccg ccagcccgat ggtgacggcg aggaccacgg tcgacgccag
      901 cagtccaccc gcgacggaca cggtggccag ctggagcagg gggatgggcg gcgtggccag
      961 caccgccccg gcgaggagcc acgccccgcc cgccagcacc agggagaccc cggacgtgag
     1021 gagcagcgcg ccgatcacgt tctcgaccac gacggtgccg cgtcgtcgcg acagggagaa
     1081 cgtcccggcg tggaccgagg tgctgagccg gctgccgagg gccgagaaca cgttgccccg
     1141 caggccgatg gcggcgggga ccaggacgag caggcccggg aggcgctcga aggtgccggt
     1201 gagggcgccg aggaagacgc cggcgacgag gctggtggtg ctgttgacgg ccagggccgt
     1261 cagtgtctgg gcgcggtcag atgaccagag gccggtgcgg gcgcgccaga gtggaacgag
     1321 aggccgtcgc atgacgactc ggaggttcct ggcaggccgg cacgacccca tcctgcccca
     1381 tccggcccac cggtccaccc catggcggcc ggtccctcca tgtgggaccg gggccggtga
     1441 tggggcagac tgccccccgt gagcggaaac atccccttcg tcgactacct ggccctggag
     1501 ccggagcccc acctcgtggc caacgagtgc acgtcgtgcg gcgcccggtt cttcgaccgc
     1561 cgcaacgcct gcgcctcgtg cttcggtacc gagttccgca aggccgacat cgccaccgag
     1621 ggcgagctgc ggtcgtacac gatcgtggcc ttcgccgccc ccggcatccc cgtgcccttt
     1681 gtggccggga tcatcgactg cgacgggact tcggtgcggg gcaatgtcat caacgtcgag
     1741 ccggacgccg agcacgtcca gctcggcatg aaggtgaagc tgacgacggt tcctgtcggt
     1801 gaggacaccg ccggcaccga agcggtgaac tacgcgttcg agcccctgga aggagcagcc
     1861 tgatggccag cgacgacatc tggatcctcg ggatccacat gaccaagttc ggcaagcggc
     1921 ccgatgacga cgtcatcgac ctggccgccg aggcctcgtt ggccgcgctg aaggacgccg
     1981 gggtgacgat ggccgacatc ggcgtgctcg ccgccggcaa cctgatgggg gccagcgccc
     2041 cgacgggcca gatgttgcag aagcagatcg gccagacggg catccccgtc ttcaacgtgt
     2101 cgaacgcctg cgccacgggc gcgaccgcgc tgcggacggc gatcatggcc atcaaggcgg
     2161 gcgagacgga catgggcctc gccgtcggcg tggagaagct gtccggcgcg ggcctgctgt
     2221 ccggaggctc ccgcaaggcg gacgccgacg agtggacgcc cagcggccgc tacggcgcgg
     2281 tggccggggt cgacggtcgc atcggcaccg agatcatgcc gggcgtcttc gcccaggtcg
     2341 ggatggagta cagccacaag tacggcggcg ccgacttcga gctcttcgcc cggatcagcg
     2401 agaagaacca cagccactcg acgctgaacc cgctggccgc ctacacgaag aagatgaccc
     2461 tcgaggagat catgggcgac gtcatgatcg cctaccccaa cacccggccg atgtgctcgg
     2521 ccaactgtga cggggccgcc gccgcggtgg tcgtgtccga cgccaagctg aagaccctcg
     2581 accccgacca gcagcgccgg gccgtgaagg tctccgcgtc cgtcctcacg tccgacccgt
     2641 gggaggaggc gtgccagatc ctccccgacg tcaacaccct cacccgccgg gctgccaccc
     2701 aggcctacga gcaggccggc gtcgctcccg aggacctcga cctggtcgag ctccacgact
     2761 gcttcgccac cgccgagctc gtccactacg acaacctcat gctctgc
//
LOCUS       DASTFV010000441         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1646907, whole genome shotgun sequence.
ACCESSION   DASTFV010000441 DASTFV010000000
VERSION     DASTFV010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646907"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..>2256
                     /locus_tag="VFP02_08140"
     CDS             62..>2256
                     /locus_tag="VFP02_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HET9543037.1"
                     /translation="MAKESDKEPGLIALALQGVKLVGVRGALRIATYGLWKARLDKRY
                     LKDRPTGPELRPGSKGEVELGGDLTRIRDEIVSATSARIPFGELSLEVVFLNLAMVRL
                     TWTPGRLPVSSAHDDTTPTEVQPFEASEDGDTTVLESGVTRVRVARDGSVVIDARPLV
                     ETAEEAAAGGWRDDWRPVRRERPPVLVGETWTATVELEPDDQVHGLGERAAPLNLRPG
                     TYGLWNTEQSGSYGPEADPLYITIPVHLVVGPGRGHLGFWENTHNGTVTIGETLSASF
                     EGGALRTYVAPGPPDRALEQFHELTGRPALPPQWALGYHQCRWGYRTEADIREVLAGF
                     REHDLPLSAIHFDIDYMDRYRVFTVNEEAFPDLARLTADLDAEGVKAVTIIDPGVAKA
                     DDFPLYREGRERGHFLKLPDGREVNAVVWPGLVGFPDFSAPAVREWWADQYPALLDRG
                     VAGIWHDMCEPAVFAAGVDASLPLATRHHLDGQGGDHVEGRNLYGQGMAQAGHDALRR
                     HRPDRRPWLLTRSGWAGVQRYAWTWTGDVATSWTMLHRTLCTVLNLTMSGIPYTGPDV
                     GGFSGDPSPELYTRWFQMSAWLPFFRSHSIFTMPSREPWNAAGPHLEAIREAMTWRCR
                     LIPYLYTQAWLTSLGGDPPILPVWWPTQGAPHEDPDLLAIDDEFLVGPDLLVAPVFES
                     GATRRAVRFPPGSWFDLVGEGRHEGVIEVEVTMQRIPVFVRAGSVVPTD"
BASE COUNT          318 a          797 c          805 g          336 t
ORIGIN      
        1 cacccctagc cggtcggcca ccgccgggcg acgctgaggg ccatggcgat ggtcgcccgt
       61 cgtggccaag gagagcgaca aggaaccggg gctgatcgcc ttggcccttc aaggcgtgaa
      121 gctggtgggc gtgcgggggg cgttgcggat cgccacctac ggcctgtgga aggcgaggtt
      181 ggacaagcgg tacctgaagg accgccccac cggacccgag ctccgtccgg gctccaaggg
      241 tgaggtagag ctcggaggtg atctgacccg gatccgagat gagatcgtct cggccacgtc
      301 ggcccggatc ccattcggtg agctgagcct ggaggtggtg tttctcaacc tggcgatggt
      361 gcgcctcacc tggacgccgg gacggctacc agtgagctca gcccatgacg acaccacgcc
      421 gaccgaggtg caaccgttcg aggcctccga ggacggagac acgaccgtgc tcgagtcggg
      481 ggtgacgcgg gtccgtgtcg cccgggacgg ctcggtggtc atcgatgccc gccccctcgt
      541 ggagaccgcc gaggaggcgg ccgcgggcgg ctggcgcgac gactggcgac cggtccgccg
      601 cgagcgaccg ccggtcctgg tcggcgagac ctggacggca acggtcgagc tcgagcccga
      661 cgaccaggtc cacggcctgg gggagcgagc cgcgccgctg aacctgcgac ccgggaccta
      721 cgggctgtgg aacaccgagc agtccggcag ctacggcccc gaggccgatc ccctctacat
      781 cacgatccct gtccacctgg tcgtggggcc tggacgcggg cacctcggct tctgggagaa
      841 cacccacaac ggcaccgtca cgatcggcga gacgttgtcg gccagcttcg aggggggcgc
      901 cctgcggacc tacgtggcgc cggggccgcc cgatcgggcc ctcgagcagt tccatgagct
      961 caccggtcga ccagccctgc ccccccagtg ggccctcggc taccaccagt gccgctgggg
     1021 ctacaggacc gaagccgaca tccgcgaggt gctggccggg ttccgcgagc acgacctgcc
     1081 cctgtcggcc atccacttcg acatcgacta catggaccgg taccgggtct tcaccgtcaa
     1141 cgaggaggcg ttcccggacc tcgcccgcct gaccgctgac ctggacgccg agggggtgaa
     1201 ggcggtgacg atcatcgatc ccggcgtggc caaggccgat gacttcccgc tctaccggga
     1261 gggccgggag aggggtcact tcctgaagct gccggacggc cgggaggtca acgccgtggt
     1321 ctggcccggc ctcgtcggct tccccgactt cagcgccccg gctgtgcgcg agtggtgggc
     1381 cgaccagtac ccggccctgc tggaccgggg cgtggccggc atctggcacg acatgtgcga
     1441 gccggccgtg ttcgccgccg gcgtcgacgc ctccctcccg ctggccacgc ggcaccacct
     1501 cgacggccag ggcggtgacc acgtggaggg tcgcaacctc tacggccagg ggatggccca
     1561 ggccggtcac gacgccctgc gccgccaccg tcctgatcgt cggccgtggc tgctgacccg
     1621 ctcgggttgg gccggggtcc agcgctacgc ctggacctgg accggcgacg tggccacctc
     1681 gtggacgatg ctgcaccgca cgctctgcac ggtcctcaac ctgacgatgt cggggatccc
     1741 gtacaccggc ccggacgtcg gtggcttctc gggggacccg tccccggagc tctacacgcg
     1801 ctggttccag atgtcggcgt ggctgccgtt cttccggtcc cactcgatct tcaccatgcc
     1861 gtcccgcgag ccgtggaacg ccgccggtcc tcacctggag gcgatccggg aggcgatgac
     1921 gtggcgctgc cgcctgatcc cgtacctcta cacccaagcc tggctgacca gcctcggtgg
     1981 tgacccgccg atcctgccgg tgtggtggcc cacgcagggc gcgccgcacg aggaccccga
     2041 cctcctggcg atcgacgacg agttcctggt cgggcctgac ctcctcgtgg cgccggtgtt
     2101 cgagtcaggc gccacgcgtc gtgccgtgcg cttcccgccc gggtcgtggt tcgacctggt
     2161 gggggaaggc cgtcacgagg gcgtgatcga ggtcgaggtg acgatgcagc ggatcccggt
     2221 cttcgtgcgg gcgggctcgg ttgtgccgac cgacga
//
LOCUS       DASTFV010000442         3576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1655265, whole genome shotgun sequence.
ACCESSION   DASTFV010000442 DASTFV010000000
VERSION     DASTFV010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655265"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VFP02_08145"
     CDS             complement(<1..904)
                     /locus_tag="VFP02_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET9543038.1"
                     /translation="MAHDRRFRFATEIHQPFPGMTWVDTAKEIEELGYSTVFFPDHFD
                     EGSGPLVAMTVCAVATSTLNVGTLVFDCDYRHPAVLARELATLDQLAEGRLEVGIGAG
                     WKKTDYDQSGIPMDRPGVRVDRMIEHCAVLKGLWADGPFSLAGEHYQIDGLDGTPAPY
                     TPGGPPILVGGGAPRLLKWAGGNADIVGVNASIHSGEVDEAAAQDAQADRIDEKVAWL
                     QEGAGDRFADLELNAWLAAAEITDDPAIAEAVAAFFGTDADSLRQSPLALVGSPGDVA
                     EMLHERRDRWGYSYHVIPGDKARDF"
     gene            complement(957..2057)
                     /locus_tag="VFP02_08150"
     CDS             complement(957..2057)
                     /locus_tag="VFP02_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9543039.1"
                     /translation="MTPTALLIGGTGPTGPLIAAGLEARGHEVTLFHSSRHEVEDVAH
                     LRHLHGDAYDRDSLVDVLADETFDVVVASYGRLRVIAELLAGRVGRFLSIGGVPVYKG
                     YFDPDAHQPAGLPVPTAEDAVLATEDDDGKSYRIGRTEEIVFEHHPTATHFRYPFVYG
                     PRQLVPREWCIVRRILDGRPFLILPDDGLSLVTFGYVDNLAHALLLAVDRPEASRGEI
                     FNCGDEECLSLRQVAELLTDELGHEWELIGMPSALATPARPLMMNHRTTHRVMDLAKL
                     RDLLGYRDAVPAREAIRRTGRWLAAHPPAAGGEEEAVLEDPFDYAAEDRLVAWWRAVI
                     ADPPDLGYTTEPGYGLAYGGPGATRVRSDDRI"
     gene            complement(2062..>3576)
                     /locus_tag="VFP02_08155"
     CDS             complement(2062..>3576)
                     /locus_tag="VFP02_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HET9543040.1"
                     /translation="GPRSASASWPVEPLPERGLGGLLAEVPAAADEPLVHEGTTVTAT
                     RADVEASVDALAAVLAGLGIGAGHAVVVALPNGVGIVAALFAVWRVGAVYVPVNPRST
                     PGEVTHVVAEVGPVAAVTTPDLADRFALPVVVADEGGWTTDAGGPVDGERRDEDIAAI
                     SFTSGTTGRPKPVLMRHSGVLELIDGVIGSLRTTPTAGPPRPPMPNLIPVSMSLWAGI
                     YQVLFAFRVGAPIVILPRFETGAFAAAVRRFGIRSTVLPPPAMAMLSDDTAIPDLAPL
                     RYVRSISSPLSPLQARRFRDRFGIAVLNGWGQTEIGGEIVGWSAADSKAFGETKLGAV
                     GRPHTGVTIKVNEDGELWVRTPSLSAGYADGADLSDRMSPDGWFRTGDVGRVDADGFV
                     WIEGRVSDMINRGGLKVFPAEVAEVLLLSPAVADAAVVGVPDDRLGEVPWAFVVPGAS
                     EPSEDALAGLCREHLAPYKVPVRFVTVDELPRNDIGKVLTRDLVTRATALREGD"
BASE COUNT          510 a         1260 c         1324 g          482 t
ORIGIN      
        1 cgaagtcgcg ggccttgtcg cccgggatca cgtggtacga gtagccccac cggtcacgac
       61 gctcgtggag catctcggcc acgtctcccg ggctgccgac cagcgccagc ggtgactggc
      121 gcaacgagtc ggcgtcggtc ccgaagaagg cggccacggc ctcggcgatc gccgggtcgt
      181 cggtgatctc ggccgccgcc agccaggcat tgagctcgag gtcggcgaag cggtcgcctg
      241 ccccctcctg cagccaggcc accttctcgt cgatgcggtc ggcctgcgcg tcctgggccg
      301 cggcctcgtc gacctcgccg gagtggatgg acgcgttgac cccgacgatg tcggcgtttc
      361 ctccggccca tttcaggagg cggggcgcac cgcctccgac caggatcggc gggcctcccg
      421 gcgtgtaagg cgcgggggtg ccgtccaaac cgtcgatctg gtagtgctca cccgcgaggc
      481 tgaacgggcc gtccgcccac agccccttca ggaccgcgca gtgctcgatc atccggtcga
      541 cccggacgcc gggacggtcc atcgggatgc ccgactggtc gtagtcggtc ttcttccacc
      601 cggcgccgat gccgacctcg aggcggcctt cggccagttg gtcgagggtg gccagctccc
      661 gggccagcac cgcggggtgg cggtagtcgc agtcgaacac gagggtcccg acgttgaggg
      721 tggacgtggc gaccgcgcac accgtcatcg cgaccagagg gccggacccc tcgtcgaagt
      781 ggtcggggaa gaacacggtc gagtagccga gctcctcgat ctccttggcg gtgtcgaccc
      841 acgtcatgcc ggggaagggc tggtggatct cggtggcgaa gcgaaagcga cggtcgtgag
      901 ccatccccgc agtctgcccc ctctccgttc tggacgcggt cggtcgccca gccggttcag
      961 atgcggtcgt cggagcggac tctcgtggcg ccggggccgc cgtaggcgag gccgtagccg
     1021 ggctcagtcg tgtagccgag gtcgggggga tcggcaatga cggcgcgcca ccaggcgacc
     1081 aggcggtcct cggcggcgta gtcgaagggg tcttcgagga cggcctcctc ctccccgccg
     1141 gcggccggcg ggtgagccgc cagccagcga ccggtgcgac ggatcgcctc gcgggcgggg
     1201 accgcgtccc ggtagcccag gaggtcccgg agcttggcca ggtccatcac ccggtgcgtc
     1261 gtgcggtggt tcatcatcag cgggcgggcc ggtgtcgcca gcgccgacgg catcccgatc
     1321 agctcccact cgtggcccag ctcgtcggtc aggagctcgg ccacctggcg gaggctcagg
     1381 cactcctcgt cgccgcagtt gaagatctcg cctctcgacg cctcgggccg gtcgaccgcc
     1441 aggagcaggg cgtgggccag gttgtcgacg taaccgaagg tgacaaggct caggccgtcg
     1501 tcgggcagga tcaggaacgg ccgcccgtcg aggatgcggc gcacgatgca ccactcccgg
     1561 ggcaccagct ggcgaggccc gtagacgaag gggtaccgga agtgtgtggc cgtcgggtgg
     1621 tgctcgaaga cgatctcctc ggtgcggccg atgcggtagc tcttgccgtc gtcgtcctcg
     1681 gtggccagca cggcgtcctc cgcggtgggt accggcaagc cggccggctg gtgggcatcg
     1741 gggtcgaagt agcccttgta gacgggcacg ccgccgatcg agaggaagcg cccgacccga
     1801 ccggccagca gctcggcgat cacccgcagc cggccgtagc tggcgacgac gacgtcgaag
     1861 gtctcgtcgg cgaggacgtc gaccagcgag tcgcggtcgt aggcgtcccc gtggaggtgg
     1921 cggaggtggg cgacgtcctc gacctcgtgg cggctgctgt ggaagagggt gacctcatgg
     1981 ccccgggcct ccaacccggc ggcgatgagg ggcccggtcg gcccggtgcc cccgatcagc
     2041 aacgcggtcg gcgtcatccg gtcagtctcc ctccctgagg gccgtggctc gggtgacgag
     2101 gtcgcgggtg aggaccttgc cgatgtcgtt gcggggcaac tcgtcgacgg tgacgaagcg
     2161 gacgggcacc ttgtaggggg cgagatgctc gcggcagaga ccggccagcg cgtcctcgga
     2221 cggctccgag gcaccgggaa cgacgaaggc ccagggcacc tcgcccaggc ggtcgtcggg
     2281 cacgcccacg acggcggcgt cggccacggc cggggacagg agcaggacct cggccacctc
     2341 ggcggggaag accttcaggc caccccggtt gatcatgtcg ctgacccggc cctcgatcca
     2401 gacgaagccg tccgcatcga cccggcccac gtcgcccgtg cggaaccagc cgtcggggct
     2461 catgcggtcg gacagatcgg ccccgtcggc gtaccccgca ctgagcgacg gcgtccgcac
     2521 ccagagctcg ccgtcctcgt tcaccttgat cgtcacaccg gtgtggggcc ggcccactgc
     2581 ccccagcttc gtctcgccga aggccttgga gtctgccgcc gaccagccga cgatctcgcc
     2641 cccgatctcg gtctgacccc agccgttgag cacggcgatg ccgaagcggt cgcggaagcg
     2701 gcgggcctgc aggggcgaca acggcgagct gatcgaccgg acgtagcgca ggggggcgag
     2761 gtccgggatg gcggtgtcgt cgctgagcat ggccatcgcc ggcgggggca ggacggtgga
     2821 gcggatcccg aaccggcgca cggcggcggc gaaggcgccg gtctcgaaac ggggcaggat
     2881 cacgatcgga gcgcccaccc ggaaggcgaa gaggacctgg tagatccccg cccacagcga
     2941 catcgacacc gggatcaggt tgggcatcgg gggtcggggc ggcccagcgg tcggcgtggt
     3001 ccgcaacgag ccgatgaccc cgtcgatcag ctccaagacc cccgagtgcc gcatgagcac
     3061 cggcttcggt cggccggtgg tgcccgaggt gaagctgatg gcggcgatgt cctcgtcccg
     3121 acgctcgccg tcgaccgggc ccccggcgtc cgtcgtccag cccccctcgt cggccacgac
     3181 caccggtagc gcgaagcggt cggccaggtc gggcgtcgtg accgcagcca ccggcccgac
     3241 ctcggccacg acatgggtga cctcccccgg ggtggagcgg gggttcaccg gcacgtagac
     3301 ggcgccgacc cgccagacgg cgaacagggc ggccacgatc cccaccccgt tgggcagggc
     3361 cacgaccacg gcgtggccgg cgccgattcc cagcccggcc agcacggccg ccagcgcgtc
     3421 gacggaggcc tcgacgtcgg cccgggtcgc cgtgaccgtg gtcccctcgt ggaccagcgg
     3481 ctcatcggcg gccgcgggga cctccgccag gaggcccccg agaccccgct caggcagtgg
     3541 ctcgaccggc cagctcgccg aggctgacct tggacc
//
LOCUS       DASTFV010000443         2639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1658828, whole genome shotgun sequence.
ACCESSION   DASTFV010000443 DASTFV010000000
VERSION     DASTFV010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658828"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFP02_08160"
     CDS             <1..573
                     /locus_tag="VFP02_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9543041.1"
                     /translation="PPIDLPGAVASNFPTDFTDNAPALAQSAIGQFEVAASPLQMALI
                     AATIANDGVTMAPHVVTELRDSDGDVVETYDPEVWLQPISAGDAATMQEAMRGVLGGT
                     ATNLAGLPGLDVGAKTGTAQLGTDPPSSHAWMIAWAGHEGSEPEIAVAVLVEGTPGVG
                     SEATGNSEAGPIARCIIATALEVDGGGGCP"
     gene            616..2178
                     /gene="pknB"
                     /locus_tag="VFP02_08165"
     CDS             616..2178
                     /gene="pknB"
                     /locus_tag="VFP02_08165"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HET9543042.1"
                     /translation="MTEPPDAVVYRGRYALQQSIGKGGMAEVFLAQDQLLDRPVAVKR
                     LSSEHAADPSFVERFRREAQAAAKLTHPNIVAVFDYFEEDGDYFIVQEFVDGRSLAEL
                     LRAEGRLHPDRAADIAADVAAGLGAAHREGMVHRDVKAGNVLVAKDGQVKVADFGIAR
                     VFAGGDSELTKAGTVMGTATYFSPEQAQGKPVDPRSDLYSLGVVLYEMLVGQPPFSGD
                     EPVAIAYAHVHEMPKALRAVDPALPVEIDAITMKLLAKEPANRYPSADDLRADLRRFR
                     EGHKIPAVAPVPLTAVLAAPPPANDVDFGSFEDGRYVEPPRRTGFFLLFMFLIMAALA
                     GGLYLLSQQLDEKNDVVTVTVPNVVGQNRQRAATDLRRASLRVEVQMQADPEVAVDVV
                     ISQDPQPDSDVAEHSVVTIVVSAGAETAQVPGVLGLDVFSATQTLQSAGFIVQQEQAE
                     DPSEQYDEGQIWFQNPPAGTTAPVGSVVIIRVVPVPPSTTTPPTLPPVTAPPATAPPV
                     TAPPTVPPTVAP"
     gene            complement(2210..>2639)
                     /locus_tag="VFP02_08170"
     CDS             complement(2210..>2639)
                     /locus_tag="VFP02_08170"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET9543043.1"
                     /translation="GPCTPNEAGISVPLIRELAGKVPILGVCLGHQCIGQVFGGRIVR
                     APRIMHGKTSEIRHEGAGVFAGLPNPLEATRYHSLVVGAASVPDVLEVTARTEDGVVM
                     GLRHRELDVEGVQFHPESILTEHGHDLLRTFLARTGAPVA"
BASE COUNT          393 a          967 c          912 g          367 t
ORIGIN      
        1 cccccgatcg acctgccggg agcggtggcc tcgaacttcc ccactgactt caccgacaac
       61 gcccccgctc tggcccagtc ggccatcggc cagttcgagg tcgcggcctc acccctgcag
      121 atggctctga tcgccgccac catcgccaac gacggcgtga cgatggcgcc ccatgtcgtc
      181 actgagctgc gggacagcga cggcgatgtc gtcgagacct acgaccccga ggtctggctg
      241 cagcccatca gcgccggcga cgcggccacg atgcaggagg cgatgcgggg tgtcctgggg
      301 gggacggcca ccaacctggc cgggctgccc ggcctcgacg tcggggccaa gaccgggacg
      361 gcccagctgg gcaccgaccc gcccagctcg cacgcgtgga tgatcgcgtg ggccggtcac
      421 gaggggagcg agcccgagat cgccgtggcc gtgctcgtcg agggcacccc cggggtgggg
      481 agcgaggcca cgggcaacag tgaggcaggg cccatcgccc gttgcatcat cgccaccgcc
      541 ctcgaggtcg acggcggcgg cggttgtccc tagggaccgt ggtcgccgac agcaccctca
      601 ctactctgtg gcgccgtgac cgagccgcct gacgctgtcg tctaccgcgg ccggtatgcc
      661 ctccagcagt cgatcggcaa ggggggcatg gctgaggtct tcctggccca ggaccagctc
      721 ctggaccggc cggtggcggt caagcgcctg tcgtccgagc acgccgccga cccgtcgttc
      781 gtcgagcgct tccgccggga ggcgcaggcc gcagccaagc tgacccaccc caacatcgtc
      841 gccgtcttcg actacttcga ggaggacggc gactacttca tcgtccagga gttcgtcgac
      901 ggtcggtcgt tggccgaact gctccgggcg gagggtcgcc tgcaccccga ccgggctgcc
      961 gacatcgccg ccgacgtcgc cgccggccta ggggccgctc accgggaagg catggtccac
     1021 cgggacgtga aggcgggcaa cgtgctggtg gccaaggacg gccaggtcaa ggtggccgac
     1081 ttcggcatcg cccgcgtgtt cgccggcggg gacagcgagc tgaccaaggc cggcaccgtc
     1141 atgggcacgg ccacctactt ctcgccggag caggcccagg gcaagcccgt cgacccccgc
     1201 agtgacctct actcactcgg cgtcgtcctc tacgagatgc tcgtgggcca gccaccgttc
     1261 tcgggcgatg agccggtggc gatcgcctat gcccacgtcc acgagatgcc caaggcgctg
     1321 cgagcggtgg acccggccct tccagtcgag atcgatgcca tcaccatgaa gctgttggcc
     1381 aaggagccgg ccaaccgcta tccgtcggcg gacgatctac gggcagacct gcgccgcttc
     1441 cgggaaggcc acaagatccc cgcggtcgcg cccgtccccc tcaccgccgt cttggcggcg
     1501 cccccgccgg ccaacgacgt cgacttcggg tcgttcgagg acggtcgcta cgtcgagccc
     1561 ccgcgccgaa ccggcttctt cctgctgttc atgttcctga tcatggcggc cctggccggc
     1621 ggcctgtacc tgctgtccca gcagctcgac gagaagaacg acgtggtcac ggtcacggtg
     1681 cccaacgtgg tcggccagaa ccgccagcgc gcggccaccg acctgcgccg ggccagcctg
     1741 cgggtcgagg tgcagatgca ggccgatccc gaggtggcgg tcgacgtggt catcagccag
     1801 gacccgcagc cggacagcga tgtcgccgag cactcggtgg tgaccatcgt ggtcagcgcc
     1861 ggagcggaga cggcgcaggt gccgggcgtg ttgggcctcg acgtgttctc ggccacccag
     1921 acgctgcagt ccgcgggctt catcgtccaa caggagcagg ccgaggatcc gtccgagcag
     1981 tacgacgagg gccagatctg gttccagaac ccacccgcgg gcacgaccgc acccgtgggc
     2041 tcggtcgtga tcattcgggt cgtcccggtg ccgccgtcga ccacgacgcc cccgacactg
     2101 ccgcccgtca cggcgccgcc cgccacggcg ccgcccgtga ccgccccccc gaccgtcccg
     2161 ccgaccgtcg ccccctgaac gcgggcccgc gcctccgttc agcggctact caggcgaccg
     2221 gcgctccggt gcgggccaag aaggtccgca gcaggtcgtg gccgtgctcg gtcaggatcg
     2281 actcggggtg gaactgcacg ccctccacgt cgagctcccg gtgacgcagg cccatgacca
     2341 cgccgtcctc ggtgcgggcg gtcacctcga gcacgtcggg gaccgaggcg gccccgacga
     2401 cgagcgagtg gtagcgggtg gcctcgagtg ggttgggaag tccagcgaac acgccggcac
     2461 cctcgtgtcg gatctcggac gtcttgccgt gcatgatgcg cggcgcacgg acgatccggc
     2521 cgccgaacac ctggccgatg cactggtggc cgaggcagac gccgaggatc ggcaccttgc
     2581 ccgccagctc gcggatcagc gggacggaga tgccggcttc gttcggagtg caggggccg
//
LOCUS       DASTFV010000444         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1660126, whole genome shotgun sequence.
ACCESSION   DASTFV010000444 DASTFV010000000
VERSION     DASTFV010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660126"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFP02_08175"
     CDS             <1..202
                     /locus_tag="VFP02_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543044.1"
                     /translation="RAVLVATGSHVLFRYASEVDHEGEHAAVVLLEGGDQAVSLGSAL
                     NPGSGGVATAQAAIRALDGLAA"
     gene            complement(265..684)
                     /locus_tag="VFP02_08180"
     CDS             complement(265..684)
                     /locus_tag="VFP02_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9543045.1"
                     /translation="MADHFVKHLATVPLFAECSKRDLAKIASLTDEIEVAAGRELIRE
                     GEVGHEAFVVVDGTASVTRDGNEVATIGPGAVFGEMALIERSPRNASVSAKTPMRLLV
                     IGKREFTGLLDEAADFRNSIMAALAARVRQKDLDLYG"
     gene            complement(751..>2277)
                     /locus_tag="VFP02_08185"
     CDS             complement(751..>2277)
                     /locus_tag="VFP02_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET9543046.1"
                     /translation="VVPNGVPAGTYGVKGTCIAPMYEDLGAVTADPFTVTGAGAPVPE
                     RAPVTPRFPSQIEPYPTYDGQSTCSPAPKPGMIAYRDMVMAAYPATSSYGISRDCNVG
                     GTSEHKEGRAWDWANDATTLAGRRRVANFFHWLFATDSYGHRHAMARRLGVMYLIWNR
                     RIFRMYRANEGWTPYTGSSPHTDHVHVSLTRRGGNGRTSFWTMQLDGPNPPPPTDPPP
                     RPRRARAEFEQTARAVDGTFEHAQVGDFDGNGKNDILWYGEGAKPEYIWWGRRGRGFV
                     VADIRAAGRFRPMVGDYDGDGRDDILWYVPGPGQDYLWYGRTDRSWDTGPTRVATRFP
                     GSAVGDFNGDGRDDVFWYGPGDGLDKLWFGRADRHFALRSLTVGGDFAPTAGDFDGDG
                     RDDVLLFGRGAGADELWFGRPDRTFDATDRTINQSSTPIVGDLDANGRDDIFWYAPGT
                     TPDRVWWGSRSRTFGAGAVTNVAGTYRPAFAGDFDEDGHDDIFWYRAGTAPDYIWWFQ
                     "
BASE COUNT          304 a          777 c          851 g          345 t
ORIGIN      
        1 ccgggcggtg ctggtcgcca ccgggagcca cgtcctgttc cgctacgcca gcgaggtcga
       61 ccacgagggt gagcacgccg ccgtcgtcct cctcgagggc ggggaccagg ccgtgtccct
      121 cggctccgcc ctcaacccgg gctccggcgg cgtggccacc gcccaagccg ccatccgcgc
      181 cctcgacgga ctcgccgcct agcccgaccc gggttcaggt tgtgcgcggc cgcggcggga
      241 cgtggtgggt gcaaccagaa cggttcaacc gtagaggtcg aggtccttct ggcggacccg
      301 ggccgcgagg gcggccatga tgctgttccg gaagtcggcg gcttcgtcga gcaggccggt
      361 gaactcccgc ttgccgatca ccagcaaccg catcggcgtc ttcgccgaca cgctggcgtt
      421 gcggggggat cgttcgatga gcgccatctc gccgaacacc gcaccgggtc cgatggtggc
      481 cacctcgttg ccgtcccggg tcaccgaggc ggtgccgtcg accacgacga aggcctcgtg
      541 gccgacctcg ccctccctga tcagctctcg tccggccgcc acctcgatct catcggtcaa
      601 cgacgcgatc ttggccaggt cacgcttcga gcactcggcg aacagcggaa cggtggcgag
      661 atgtttgacg aagtggtcgg ccatggccgc ggatagtacg aggccacgct gggtggcgct
      721 gtccgaccga gctcgccggc ggcaaaccga ctactggaac caccagatgt agtccggggc
      781 cgtaccggcc cggtaccaga agatgtcgtc gtggccgtcc tcgtcgaagt cgccggcgaa
      841 ggcgggccgg taggtgcccg ccacattggt gacggcgccg gcgccgaagg tgcggctccg
      901 agagccccac cagaccctgt ccggggtcgt gccgggggcg taccagaaga tgtcgtcgcg
      961 accgttggcg tcgaggtcgc cgacgatggg cgtcgagctc tggttgatgg tgcggtcggt
     1021 ggcgtcgaac gtccggtccg ggcgaccgaa ccagagctcg tcggccccgg cgccccgccc
     1081 gaagagcagg acgtcgtcgc ggccgtcgcc gtcgaagtcg cccgcggtcg gcgcgaagtc
     1141 gccgcccacg gtcagggaac ggagggcgaa gtgtcggtcc gctcgcccga accagagctt
     1201 gtcgaggccg tcgccgggcc cgtaccagaa cacgtcgtcg cgcccgtccc cgttgaagtc
     1261 acccacggcg gagccgggga accgcgtggc gacgcgcgtg ggcccggtgt cccacgaccg
     1321 gtcggtccgg ccgtaccaga ggtagtcctg gcccggtccg gggacgtacc agaggatgtc
     1381 gtcgcgcccg tccccgtcat agtcgccgac catcggacgg aagcgaccgg ctgcccggat
     1441 gtcggccacg acgaagcccc ggccccgtcg gccccaccag atgtactccg gcttggcccc
     1501 ttcgccgtac cagaggatgt cgttcttgcc gttgccgtcg aagtcgccga cctgggcgtg
     1561 ctcgaacgta ccgtcgacgg cccgcgcggt ctgctcgaac tcggcccggg cccgcctggg
     1621 acggggcggg ggatcggtcg gcggcggcgg gttcggcccg tccagctgca tggtccagaa
     1681 gctggtccgc ccgttgccgc cccgccgggt gaggctgacg tgcacgtggt cggtgtgggg
     1741 gctggagccg gtgtagggcg tccacccttc gttggcccgg tacatccgga agatccggcg
     1801 gttccagatg aggtacatga cgccgaggcg gcgggccatg gcgtgccggt ggccgtagct
     1861 gtcggtggcg aacagccagt ggaagaagtt ggcgacccgg cggcggccgg ccaaggtggt
     1921 ggcgtcgttg gcccagtccc aggcccgccc ttccttgtgc tcgctggtcc ccccgacgtt
     1981 gcagtcccgg ctgatgccgt agctggaggt cgccgggtag gccgccatga ccatgtcgcg
     2041 gtaggcgatc atgcccggct tgggcgcggg gctgcaggtg ctctggccgt cgtaggtcgg
     2101 gtagggctcg atctggctgg ggaagcgggg cgtaaccggt gcccgctcgg gcacgggggc
     2161 gccggcaccg gtgacggtga aggggtcggc ggtgaccgcg cccaggtcct cgtacatggg
     2221 ggcgatgcag gtgcccttga cgccgtaggt gccggcgggg acgccgttgg ggacgac
//
LOCUS       DASTFV010000445         3575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1660502, whole genome shotgun sequence.
ACCESSION   DASTFV010000445 DASTFV010000000
VERSION     DASTFV010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660502"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..80)
                     /locus_tag="VFP02_08190"
     tRNA            complement(7..80)
                     /locus_tag="VFP02_08190"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44..46),aa:Pro,seq:ggg)
     gene            complement(105..305)
                     /locus_tag="VFP02_08195"
     CDS             complement(105..305)
                     /locus_tag="VFP02_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543047.1"
                     /translation="MPWCDDCQKFWNPNSMPENGECPTCQRVISLPRNKTPWHFKLLV
                     VAISLYLGFRLLQGIVWVAHQF"
     gene            complement(369..1898)
                     /locus_tag="VFP02_08200"
     CDS             complement(369..1898)
                     /locus_tag="VFP02_08200"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HET9543048.1"
                     /translation="MTADQERLAKATERALAGNLERVGDKLQRQNKLFVRDRLALLLD
                     AGSFIEDALLANTSAEDLPADGVVTGTGTVDGRPVAVVANDPTVKAGSWGARTVEKIV
                     RLTELALRHELPVFWLIDSAGARITDQVELFPGRRGAGHIFYNQVRLSGRVPQICCLF
                     GPSAAGGAYIPSFCDIVIMVEGNASMYLGSPRMAEMVIGEVTTLEEMGGARMHATVSG
                     CGDNLATDDEDAIDQAVAWFSYFPQNWREAPPSYEARPPAAELTAAIVPAEEQMAYDM
                     HRLVDGLVDAESFFELKPLFAPELIVGLGRLEGRPVGIVANNPMQKGGVLFGDSADKA
                     ARFVWCCDAFNIPLVFLCDVPGFMVGTQVERDGIIRHGAKMVTAVSEATVPKISVVVR
                     KAYGAGLYAMSGPAFGPEATLALPTARIAVMGPEAAVNAVFANKIAEIEDPDEREAFI
                     AEQRQIYEEDVDLLRLASELVVDAVVDFGDLRHEVVRRLDRAQGKDRHFSDRRHGVPP
                     V"
     gene            complement(1901..2977)
                     /locus_tag="VFP02_08205"
     CDS             complement(1901..2977)
                     /locus_tag="VFP02_08205"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HET9543049.1"
                     /translation="MVIPVLATTAVLCLAAALSWGRSARFRHRVWRTLDSSDSDEGAI
                     ALAWGVYRKDVHAAAFYAIAGVAIGALAFIDPPSGAAILGVLAVPAIVSLAMWRTFVN
                     EARLAQSRIGMERRAVEVLDQEERAPRMWAERLAPESLPDLVGFEVGRVYQAGSGMMA
                     GDFYDFFRLTPTRVAAVIGDVAGHGIEASITAFQAKYLLRVFLRQFKDPAQAIEELNS
                     QMYALGRDEEFISLVVVVFDTRAGTLRYTSAGHPAAYLWHDRELQPLRATGPLLMLAP
                     ASEYFSREIGLDADDLCLLYTDGLAEARDGENLFGEERIAAAVRRDPGVGTEVLCKSL
                     LEAARDFSNGPISDDVAIMAIRRE"
     gene            complement(3023..>3575)
                     /locus_tag="VFP02_08210"
     CDS             complement(3023..>3575)
                     /locus_tag="VFP02_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET9543050.1"
                     /translation="SVVRALRAIDTADVVLLVIDALIGVTHQDQRLAERASISGSPVV
                     VLLNKWEMADADQRELVSEQVDDRLKFLPNAKVLKISALTGKGTHKIIDALLDTADTY
                     HRRIPTAKVNEVLRQAQAHQPAPGGTRVLYATQGATDPPTFTLFANRRLPPTYLRYLE
                     RKLREAFAMGDTPLKLRVRARRD"
BASE COUNT          542 a         1190 c         1321 g          522 t
ORIGIN      
        1 cctcggtcgg gctgccggga tttgaacccg ggacctcttg tcccccagac aagcgcgcta
       61 ccaagctgcg ccacagcccg tgttgtggac cggcagcgta ccggtcagaa ctggtgagcg
      121 acccagacga tcccctgcag gaggcgaaag ccgaggtaga ggctgatggc cacgaccagg
      181 agcttgaagt gccacggcgt cttgttccgg ggcagggaga tgacccgctg gcaggtcggg
      241 cactcgccgt tctccggcat ggagttcggg ttccagaact tctgacagtc gtcgcaccag
      301 ggcatcggcc accactctcg cggctcgagg gatcagcgtg ccaagccgcg cctgctcagc
      361 accagggctc agacgggtgg gacgccgtgg cggcggtcgg agaagtggcg gtccttgccc
      421 tgggcccggt ccaggcggcg caccacctcg tggcggaggt cgccgaagtc gacgacggcg
      481 tcgaccacca gctcggaggc gaggcggagc aggtccacgt cctcctcgta gatctggcgc
      541 tgctcggcga tgaaggcctc ccgctcgtcg gggtcctcga tctcggcgat cttgttggcg
      601 aagacggcat tgacggcggc ctccgggccc atgaccgcga tgcgggcggt gggcagggcg
      661 agggtggcct cggggccgaa cgcgggaccg gacatggcgt agaggccggc accgtaggcc
      721 ttgcgcacca ccaccgagat cttgggcacc gtggcctcgg agacggcggt caccatcttg
      781 gccccgtggc ggatgattcc gtcccgctcc acctgcgtgc cgaccatgaa gccggggacg
      841 tcgcagagga acaccagcgg gatgttgaag gcgtcacagc accagacgaa gcgggccgcc
      901 ttgtcggccg agtcgccgaa gaggacgccg cccttctgca tcgggttgtt ggccacgatg
      961 ccgaccgggc gaccctcgag gcggccgagg ccgacgatca gttccggggc gaacaagggc
     1021 ttcagctcga agaaggactc ggcgtcgacc agcccgtcga ccagccggtg catgtcatag
     1081 gccatctgct cctcggcggg gacgatggcg gcggtcagct cggcggccgg cgggcgggcc
     1141 tcgtaggacg ggggcgcctc tcgccagttc tgcgggaagt agctgaacca ggccacggct
     1201 tggtcgatgg cgtcctcgtc gtcggtggcc aggttgtcgc cacagcccga caccgtggcg
     1261 tgcatgcggg ccccacccat ctcctccagg gtcgtcacct cgccgatgac catctcggcc
     1321 atgcggggcg agcccaggta catcgaggcg ttgccctcga ccatgatcac gatgtcgcag
     1381 aacgacggga tgtaggcccc gccggcggcc gacgggccga acaggcaaca gatctggggt
     1441 acgcgacccg agagccggac ctggttgtag aagatgtgcc cggcgccgcg ccggccgggg
     1501 aagagctcga cctggtcggt gatgcgggcc ccggccgagt cgatgagcca gaacaccggc
     1561 agctcgtggc ggagggccag ctcggtcagg cggacgatct tctcgacggt ccgggcgccc
     1621 cacgatccgg ccttcaccgt cggatcgttc gccaccacgg ccacgggccg gccgtcgacc
     1681 gtgccggtcc ccgtcaccac cccgtcggcg ggcaggtcct cggccgaggt gttggccagc
     1741 agggcgtcct cgatgaagga gccggcgtcc aggagcaggg ccagccggtc ccgcacgaac
     1801 agcttgttct ggcgctggag cttgtcgccg acgcgctcga ggttcccggc gagggcccgt
     1861 tcggtggcct tggccaggcg ctcctggtct gcggtcacgg ctactcgcgg cggatggcca
     1921 tgatcgcgac gtcgtcgctg atcgggccgt tggagaagtc ccgggccgct tccagcaggg
     1981 acttgcagag cacctcggtg cccaccccgg ggtcgcggcg gacggcggcg gcgatgcgct
     2041 cctcgccgaa caggttctcg ccgtcccggg cttcggccag gccatcggtg tagagcaggc
     2101 agaggtcgtc ggcgtcgagg ccgatctccc ggctgaagta ctcactggcc ggggccagca
     2161 tgagcagggg gccggtggcc cgcagcggct gcagctcccg gtcgtgccac aggtaggcgg
     2221 cgggatggcc ggccgaggtg tagcgcagcg tccccgcccg ggtgtcgaag accacgacca
     2281 cgagggagat gaactcctcg tcgcggccga gggcgtacat ctggctgttc aactcctcga
     2341 tggcctgggc cgggtccttg aactgacgga ggaacacccg cagcaggtac ttggcctgga
     2401 acgcggtgat ggaggcctcg atgccgtggc cggccacgtc accgatgacc gccgcgaccc
     2461 gggtcggggt caggcggaag aagtcgtaga agtcgccggc catcatcccc gagccggcct
     2521 ggtagacccg gccgacctcg aagccgacca ggtcgggcag agactcgggc gccaggcgct
     2581 cggcccacat gcggggggcc cgttcctcct ggtccagcac ttcgaccgcc cggcgttcca
     2641 tgccgatgcg gctctgggcc aggcgggctt cgttcacgaa ggtgcgccac atcgccaacg
     2701 acacgatggc ggggacggcg aggaccccca ggatggccgc gccggagggc gggtcgatga
     2761 acgccagggc tccgatggcc acgccggcga tggcgtagaa ggcggcggca tggacgtcct
     2821 tgcggtagac gccccaggcc agcgcgatgg cgccctcgtc ggagtcggag ctgtccaggg
     2881 tccgccagac gcgatggcga aagcgggccg agcgtcccca ggacagcgcg gcagccaggc
     2941 agagcacggc ggtggtcgcg aggaccggga tcaccatgac gccccgatcg tactcgccgc
     3001 cccggacggg cttgtggtcc gatcagtcac gtcgcgcccg gacccgcagc ttgaggggcg
     3061 tgtcgcccat ggcaaaggcc tcccgcagct tccgttccag gtagcgcagg taggtgggcg
     3121 ggagccgccg gttggcgaac agggtgaacg tcggcgggtc ggtggccccc tgggtggcgt
     3181 agaggacccg ggtgcctccc ggagccggct ggtgggcctg cgcctggcgg agcacctcgt
     3241 tgaccttcgc cgtcgggatg cgccggtggt aggtgtcggc ggtgtccagc agggcatcga
     3301 tgatcttgtg ggtgcctttg cccgtcaggg cgctgatctt gaggaccttg gcgttgggca
     3361 ggaacttcag ccggtcgtcg acctgctcgg acaccagctc ccgctgatcg gcatcggcca
     3421 tctcccactt gttgaggagc acgaccaccg gcgacccgga gatggacgcc cgctccgcca
     3481 ggcgctggtc ctggtgggtg acgccgatca gcgcgtcgat cacgagcagg accacgtcgg
     3541 cggtgtcgat cgcccgtagg gcccggacca cggag
//
LOCUS       DASTFV010000446         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1660603, whole genome shotgun sequence.
ACCESSION   DASTFV010000446 DASTFV010000000
VERSION     DASTFV010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660603"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /locus_tag="VFP02_08215"
     CDS             <1..1297
                     /locus_tag="VFP02_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET9543051.1"
                     /translation="GQEAKLILQVAVPLGFDPTDFDPGDDDAPGAGVDLTAGLDENAP
                     ALFNSFLFARLAESRLAQNVHQADLQVVCESEKATGGGWSFDGDPAGANAADLDTTGF
                     AVMALVAAGIDPSDPVLEAAETFVLGGLQANGSWASFGSDDPNATALAMFAVTALGGG
                     LDDLAHDPVAWLQSQQLTAPPADDGRFTSPNDGFGVNTFATSQAVQALLLDQDPGADW
                     LPVAPPASSVERRCLPASDFADVPVAAWYDDGARWVDDAQIVGGISGDLRPAGRVNRA
                     QAAMWLNDMFGSPGGAPNGFTDVPDGVWYGDGVDFVAGAPNGTIANGFGDQFRPRLTL
                     NRAQAVSWLYAAAGSPDVSTLPAHGFADVGSTTWFRDAATWAAANGIVLGFPNGTFRG
                     NDNVTRAQFAQWMFNLVATPDAWPLTATTPPTLLSSVFP"
     gene            1294..>2047
                     /locus_tag="VFP02_08220"
     CDS             1294..>2047
                     /locus_tag="VFP02_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543052.1"
                     /translation="MNRSRAVRAAVLGALVGVLAVAGMVGSAFSAPGREAQATHRAAI
                     VVDTGSAVVTRCVTFSEDSISGITALQRAGLAPVVRSFSGNGGAVCGLNGLGCPADSS
                     CLTCQAPNYWAYYRADDGAGAFSYSPVGAGSAQVTDGDVEGWRWGSGAGPGFRSFASI
                     CPLQPPLTTTTTTTAAPRPGGNGPGNGSTGSPAGGPGPGGPPGDTAPGDSSTTVAPTT
                     GGVGEGRTAPGGQDTDPAEGDAAEDVEGEEAAA"
BASE COUNT          265 a          747 c          744 g          291 t
ORIGIN      
        1 cggccaggag gcgaagctca tcctccaggt cgccgtgccg ctgggcttcg acccgacgga
       61 cttcgacccg ggtgacgacg acgcccccgg tgccggtgtc gacctgaccg ccggcctcga
      121 cgagaacgcc ccggcgctct tcaactcgtt cctgttcgcc cggctggccg agtcgcggtt
      181 ggcccagaac gtccaccagg ctgacctgca ggtggtgtgc gagtcggaga aggcgacggg
      241 agggggctgg agcttcgacg gtgatcccgc gggggcgaac gccgccgacc tcgacaccac
      301 agggttcgcc gtgatggccc tcgtggccgc cggcatcgac cccagcgacc ccgtcctcga
      361 ggcggccgag accttcgtgc tcggcggcct gcaggccaac ggctcgtggg cgtcgttcgg
      421 ctcggacgat ccgaacgcca cggccctggc catgttcgcg gtcaccgccc tgggcggcgg
      481 cttggacgat ctcgcccacg acccggtggc ctggctgcag tcccagcagc tcacggcccc
      541 cccggccgat gacggtcgct tcaccagccc caacgacggc ttcggcgtga acacgttcgc
      601 caccagccaa gccgtgcagg ccctgctgtt ggaccaggac ccgggggccg actggctccc
      661 agtggctccg ccggcttcca gcgtcgagcg tcggtgcctg ccggcgtcgg acttcgccga
      721 cgtgccggtg gccgcgtggt acgacgacgg cgcccgctgg gtcgacgacg cccagatcgt
      781 gggtggcatc agcggcgacc tgcgtcccgc gggccgggtg aaccgggccc aggccgcgat
      841 gtggctgaac gacatgttcg gaagccctgg cggagccccc aacggcttca ccgacgtgcc
      901 ggacggtgtc tggtacgggg acggcgtgga cttcgtcgcc ggcgccccga acgggacgat
      961 cgccaacggc ttcggtgacc agttccggcc ccggttgacg ctgaatcggg cccaggccgt
     1021 gtcgtggctc tacgccgcgg ccggctcacc ggacgtgagc accctgcccg cgcacggctt
     1081 cgctgacgtc gggtcgacga cctggttccg ggacgcggcg acgtgggcgg cggccaacgg
     1141 catcgtgctc ggcttcccga acggcacctt ccggggcaac gacaacgtga cccgggccca
     1201 gttcgcccag tggatgttca acctggtggc cacccccgac gcctggccgc tgacggcgac
     1261 cacgccgccg accctgttgt cctcggtctt cccgtgaacc ggtcccgcgc cgtgcgggcc
     1321 gccgtcctgg gggccctggt gggcgtcctg gcggtggccg gcatggtcgg gtcggccttc
     1381 agtgccccgg ggcgggaggc tcaggccacc catcgggcgg cgatcgtggt cgacaccggt
     1441 tccgcggtcg tcacccgctg cgtgacgttc agcgaggact ccatcagcgg gatcaccgca
     1501 cttcagcggg ccggattggc cccggtcgtg cgatccttct cgggcaacgg tggtgcggtc
     1561 tgcggactga acgggctggg ttgcccggcc gacagctcct gcctgacctg tcaggcaccc
     1621 aactactggg cctactaccg ggccgacgac ggcgccggtg ccttcagcta ctcgccggtg
     1681 ggagcgggca gcgcccaggt cacggatggc gatgtggagg gatggcgctg gggctccggc
     1741 gccggaccgg ggtttcggtc cttcgcctcg atctgccccc tacagccgcc gttgaccacg
     1801 accaccacga ccacggccgc ccctcggccg ggcggcaatg gcccggggaa cggctccacc
     1861 ggcagcccgg cgggcggtcc cggaccgggt gggccaccgg gcgacactgc accgggcgat
     1921 tcgtcgacga ccgtggcccc cacgaccggc ggggtggggg aggggagaac cgcccctggt
     1981 ggtcaggaca ccgatcccgc cgaaggcgat gccgccgagg acgtagaggg tgaggaggcc
     2041 gcggccc
//
LOCUS       DASTFV010000447         5196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1665141, whole genome shotgun sequence.
ACCESSION   DASTFV010000447 DASTFV010000000
VERSION     DASTFV010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1665141"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFP02_08225"
     CDS             <1..305
                     /locus_tag="VFP02_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HET9543053.1"
                     /translation="GQTEARIRDLEAMIERAEIVEGGSDDGTVAAGSVVSIRYEGDDD
                     VETYLLGSIEEKRDGIEIMSPGSPIGKALVGAQAGDVVEAETPGGTLRVEIVSVGG"
     gene            416..1162
                     /locus_tag="VFP02_08230"
     CDS             416..1162
                     /locus_tag="VFP02_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9543054.1"
                     /translation="MLLHGWTATADLNWFPAYEALAENCRVVALDHRGHGQGIRTARR
                     FRLADCADDAVALADVLGIDRFIPVGYSMGGLIAQLAWHRHPGRVGGLVLGATARNFR
                     GNRAAAGGFTALGAMAAAARLVPSSVRTGAAQRVIHRRPGVSDWARGEFEAADPRMVL
                     EAGQAIGAFSSHAWIGEVNVPTAVIVTEADRVVPPARQAKLAAAIPGATVHPLPMDHG
                     GCVLEPELFVPVLKDAVTDVVRRAGAFSSR"
     gene            complement(1149..2693)
                     /locus_tag="VFP02_08235"
     CDS             complement(1149..2693)
                     /locus_tag="VFP02_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HET9543055.1"
                     /translation="MTDAPMLAAIDIGTNSVHLVVARAAATDRFEIVAREKDTVRLGS
                     GGGEMKWLDDDAVDRAVAALGRQRAIAEAYGASVRAVATSAVREAENRHVFLRRAVVE
                     AGVRVEVISGFEEARLIHLGVLQAVPVYDRRVLIVDIGGGSTEVVLGERDESLVARSL
                     KLGAIRLTDRFFPDDTIKARAVTRCRRHIQGVVAPMQREIDRFDVAVASSGTAQSVAA
                     MALAARGADPAQNLNQVVITTAELAAVVEALLAAPTVQERRRIPGLDAARADIIVAGA
                     LVLQDVLAAFGATELMISESALREGVLVDTYQRTHGGSLHHLRDIRRRGVLHLAQLTD
                     EDPEHSARSADLALDLFDQLAPWHGLDDGPREYLEAAALLCNVGLFISHSGHHKHSYY
                     VIRNSEHLTGFTDNEIEVIAQVARYHRKSAPKPKHEAFAGLDEADQAVVRTLAGILRV
                     AIGLDRAHRGSVVALDCRAEGDGLVIEVEAAAGVDTTVEIWSADERKGLLVDVLNRPV
                     EVLSGS"
     gene            complement(2690..3313)
                     /locus_tag="VFP02_08240"
     CDS             complement(2690..3313)
                     /locus_tag="VFP02_08240"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9543056.1"
                     /translation="MGTIDKGVRVLGAVEEAGPCTLADLVATTGFSRATAHRLAVALE
                     AHGLLRRTADGRFALGLRLVGLGRAAADGLPLADAAGPALAALREATGESVQLYVRQG
                     EQRVCVASLESPHGLRTIVPMGAALPLDRGSAGRVLRTAPDGWVESIEERERGVASVS
                     AAVRGPSGEVLAAVGVSGPVERTTHAPGARYGAAVEAAAHAIEAALR"
     gene            3389..4789
                     /gene="leuC"
                     /locus_tag="VFP02_08245"
     CDS             3389..4789
                     /gene="leuC"
                     /locus_tag="VFP02_08245"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET9543057.1"
                     /translation="MAKTLSEKVWDRHVVHRGDGTGEPDLLYIDLHLVHEVTSPQAFD
                     GLRIAGRPVRRPDLTVATEDHNVPTTLGPIEDPISLTQVETLRSNTAEFGIRLHSMGD
                     PNQGIVHIIGPEQGLSQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLPQ
                     RRPGTMAVTVDGDLPAGVTAKDIVLAIIGRIGTGGGIGSVIEYRGSAIRALSMEGRMT
                     VCNMSIEAGARAGLIAPDETTFAYLKGRPSAPSGADWDAAVADWRTLVSDDDATFDKE
                     VVIDAATLSPHVTWGTNPAQVIPLDAAVPSPEDFAEASERDAAERALAYMGLHAGTPM
                     REVAVDTVFIGSCTNGRIEDLRAVAAVAEGRQVADGVRTLVVPGSGRVKEQAEAEGLD
                     QILTAAGFDWRQPGCSMCLAMNPDKLAIGERAASTSNRNFEGRQGRGGRTHLVSPEVA
                     AATAIAGTFATPDDLD"
     gene            4800..>5196
                     /gene="leuD"
                     /locus_tag="VFP02_08250"
     CDS             4800..>5196
                     /gene="leuD"
                     /locus_tag="VFP02_08250"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET9543058.1"
                     /translation="MEAVNVVTGTAVPLDRSDVDTDQIIPSDWLKRVERTGVGRGLFA
                     EWRDDRDFVLNDEQYAGASILVAGPNFGTGSSREHAVWAIQDYGFRAVISPRFADIFR
                     NNATKCGLVPVQVDAAVGEALMRAVEADPT"
BASE COUNT          732 a         1909 c         1858 g          697 t
ORIGIN      
        1 agggccagac cgaggcccgg atccgtgacc tcgaggccat gatcgagcgg gccgagatcg
       61 tcgagggtgg gtccgacgac ggcacggtgg ccgccgggtc ggtggtgtcg atccgctacg
      121 agggcgacga cgacgtcgag acctacctgc tcgggtcgat cgaggagaaa cgggacggca
      181 tcgagatcat gtcgcccggc tcacccatcg gcaaggcgct cgtcggggcc caggccggcg
      241 acgtcgtcga ggccgagacc cccggaggga ccctgcgcgt cgagatcgtc tcggtggggg
      301 gctagccatc accgtcccgc ccctccctcc tggtggaccc gtcgcgctgc ccgggcgggg
      361 gaccaccttc caccgcgatc tccccagtcg ggccggggcc gacgccccga cggtcatcct
      421 cctccacgga tggaccgcta cggccgacct gaactggttc ccggcctacg aggccctggc
      481 cgagaactgt cgggtcgtgg ccctcgacca tcggggtcac ggccagggca tccgcaccgc
      541 ccgccgcttc cgcctggccg actgcgccga cgacgctgtg gctctggccg acgtgctcgg
      601 catcgaccgc ttcatcccgg tcggctattc gatgggtggg ctgatcgccc agctcgcctg
      661 gcaccggcac cccggccggg tcggtggcct cgtgctggga gccactgccc gcaacttccg
      721 cggcaaccgg gccgccgccg gagggttcac cgcgctgggg gcgatggccg ccgccgcccg
      781 cctggttccc tcctcggtcc ggaccggcgc ggcccagcgg gtcatccacc gccgacccgg
      841 tgtgtcggac tgggcccggg gggagttcga ggcggccgac ccccgcatgg tgctggaagc
      901 gggccaggcc atcggggctt tctcctccca tgcctggatc ggcgaggtca acgtgcccac
      961 cgccgtcatc gtgaccgagg ccgaccgggt cgtcccgccg gcccgtcagg ccaagctggc
     1021 tgcggcgatt cctggcgcca ccgtgcaccc cctaccgatg gaccacggtg gatgtgtgct
     1081 ggagcccgag cttttcgtgc cggtgctcaa ggacgcggtg acggacgtgg tccggcgagc
     1141 gggcgccttc agctcccgct gagcacctcg acgggccggt tcaggacgtc gaccagcagg
     1201 cccttgcgct cgtcagccga ccagatctcc acggtggtgt cgacgccggc tgcggcctcg
     1261 acctcgatca ccaggccgtc cccttctgca cggcagtcca gggcgacgac actgccccgg
     1321 tgggcccggt cgaggccgat ggccacccgg aggatcccgg ccagcgtgcg caccaccgcc
     1381 tggtcggcct cgtccaggcc ggcgaacgcc tcgtgcttgg gcttgggggc actcttgcgg
     1441 tggtagcggg cgacctgggc gatcacctcg atctcgttgt cggtgaagcc ggtcaggtgc
     1501 tcggagttcc ggatcacgta gtagctgtgc ttgtggtgcc ccgagtggga gatgaagagg
     1561 cccacgttgc agagcagggc cgccgcctcc aggtattcgc ggggaccgtc atcgaggccg
     1621 tgccacgggg ccagctggtc gaagaggtcg agggcgaggt cggcggagcg ggccgagtgc
     1681 tcggggtcct cgtcggtgag ctgggccagg tgcaggacgc cacgtcgacg gatgtcgcgc
     1741 aggtggtgca gcgagccccc atgggtgcgc tggtaggtgt cgaccaggac gccttcccgc
     1801 agggccgact ccgagatcat cagctcggtg gccccgaagg cggccagcac gtcctgcagg
     1861 accagggccc cggcgacgat gatgtcggcc cgggcggcat ccagcccggg gatgcggcgc
     1921 cgctcctgca ccgtcggggc ggcgagcagg gcctcgacga cggcggccag ttcggccgtg
     1981 gtgatcacca cctggttgag gttctgggcc gggtccgccc ctcgggccgc gagggccatg
     2041 gccgccacgc tctgcgccgt gcccgagctg gccacggcga cgtcgaaacg gtcgatctca
     2101 cgctgcatcg gggcgaccac gccctggatg tggcggcggc accgggtcac ggcccgggcc
     2161 ttgatcgtgt cgtcggggaa gaagcggtcg gtcaggcgga tggcgccgag cttgagactg
     2221 cgggctacga gggactcgtc ccgctcgccg aggaccacct cggtgctgcc cccgccgatg
     2281 tcgacgatga gcacccggcg gtcgtacacg gggacggcct ggagcacccc gaggtggatg
     2341 aggcgggcct cctcgaaccc ggagatgacc tcgacgcgga cgccggcctc caccaccgcc
     2401 cgccgcagga agacgtggcg gttctcggcc tcccgcaccg cgctcgtggc gacggcccgg
     2461 accgacgccc cgtaggcctc ggcgatggcc cgctggcgcc cgagggccgc caccgcccgg
     2521 tcgacggcat cgtcgtccag ccacttcatc tccccgccgc cggagccgag gcggaccgtg
     2581 tccttctccc gggcgacgat ctcgaagcga tcggtggccg cggcccgggc caccaccagg
     2641 tggaccgagt tcgtcccgat gtcgatggcc gccagcatcg gcgcgtcggt caccggagcg
     2701 ccgcctcgat ggcgtgcgcg gccgcctcga ccgcagcgcc gtagcgcgcg cccggggcgt
     2761 gggtggtgcg ctcgaccggc ccggagacac ccacggcggc gaggacctcg ccgctggggc
     2821 cccgcacggc tgcgctcacc gaggccacgc cccgctcacg ctcctcgatg ctttccaccc
     2881 agccgtcggg ggccgttcgg aggacccgtc cggcggagcc acggtcgagc ggaagggcgg
     2941 cacccatcgg gacgatcgta cggaggccgt ggggcgactc cagggacgcc acgcagaccc
     3001 gctgctcacc ctggcggacg tagagctgga cgctctcgcc agtggcctcc cgcagcgcgg
     3061 ccagcgccgg gccggcggcg tcggccaggg gcaaaccgtc ggccgccgcc cggcccagcc
     3121 cgaccaggcg aaggccgagg gcgaagcggc cgtcggcggt gcggcgcagg agcccgtggg
     3181 cctcgagggc gaccgccagg cggtgggcgg tggcccggga gaagcccgtg gtggcgacga
     3241 ggtcggccag ggtgcagggc ccggcttcct cgaccgcgcc gagcacgcgc acccccttgt
     3301 cgatcgtgcc cacaccgctt accctgggtt ccacaacaca agatagtagt ctcatcattc
     3361 gagacagggc gaccacccga ggatcctgat ggccaagacg ctctcggaga aggtgtggga
     3421 ccgccacgtc gtgcaccgtg gcgacggcac cggcgagccc gatctgctct acatcgacct
     3481 gcacctggtc cacgaggtga cctcacccca ggccttcgac ggcctgcgca tcgccggtcg
     3541 gcccgtgcgc cgaccggacc tgaccgtcgc caccgaggac cacaacgtcc ccaccaccct
     3601 cgggccgatc gaggacccca tctccctgac ccaggtcgag accctgcggt ccaacaccgc
     3661 cgagttcggg atccgcctcc actcgatggg ggacccgaac cagggcatcg tccacatcat
     3721 cggcccggag cagggcctga gccagcccgg gatgaccatc gtctgcggcg actcgcacac
     3781 ctcgacccac ggggccttcg gcgcgctggc cttcggcatc ggtaccagcg aggtcgagca
     3841 cgtcctggcc acccagaccc tcccccagcg ccgacccggg accatggccg tcaccgtcga
     3901 cggcgacctg cccgccgggg tcacggccaa ggacatcgtc ctggcgatca tcggtcggat
     3961 cggcaccggc ggcggaatcg gctccgtgat cgagtaccgg ggctcggcca tccgggccct
     4021 gtcgatggaa gggcgcatga ccgtctgcaa catgtccatc gaggccggag cccgggccgg
     4081 cctgatcgcg cccgacgaga ccacgttcgc ctatctgaag ggacgcccgt ccgctccgtc
     4141 gggcgccgac tgggacgccg cggtcgccga ctggcgcacg ctggtcagcg acgacgacgc
     4201 caccttcgac aaggaggtgg tcatcgacgc cgccaccctg tcgcctcacg tcacctgggg
     4261 cacgaacccg gcccaggtca tccctctgga cgccgcggtg ccctcccccg aggacttcgc
     4321 cgaagcctcc gagcgcgacg ccgccgagcg ggcgctggcc tacatgggcc tgcacgccgg
     4381 caccccgatg cgggaggtgg cggtggacac cgtcttcatc gggtcctgta ccaacggtcg
     4441 catcgaagac ctgcgggccg tggccgcggt ggccgagggc cgtcaggtgg ccgacggcgt
     4501 gcgcacgctg gtcgtccccg gttccggccg ggtcaaggaa caggccgagg ccgagggact
     4561 cgaccagatc ctgacggccg cgggattcga ctggcgacag cccggctgtt cgatgtgcct
     4621 ggccatgaac ccggacaagc tggccatcgg cgaacgggcc gcgtcaacca gcaaccgcaa
     4681 cttcgagggt cgacagggcc gaggggggcg cacccacctc gtgtccccgg aggtggccgc
     4741 ggcgaccgca atcgccggca ccttcgccac ccccgacgac ctggactagg agcgacccga
     4801 tggaagccgt gaacgtggtg acggggacgg cggtcccctt ggaccggtcg gacgtggaca
     4861 ccgaccagat catccccagc gactggctca agcgcgtgga gcgcaccggt gtcggtcggg
     4921 gactcttcgc ggagtggcgg gacgaccggg acttcgtgct caacgacgag cagtacgcgg
     4981 gggcgtcgat cctcgtggcc ggcccgaact tcggcaccgg ctcctctcgc gagcacgccg
     5041 tgtgggccat ccaggactac ggcttccggg ccgtgatctc gccccgcttc gccgacatct
     5101 tccgcaacaa cgccaccaag tgcggcctcg tgcccgtgca ggtggacgcg gccgtgggcg
     5161 aggccctgat gcgcgcggtg gaggccgacc cgacgc
//
LOCUS       DASTFV010000448         4646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1668104, whole genome shotgun sequence.
ACCESSION   DASTFV010000448 DASTFV010000000
VERSION     DASTFV010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668104"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1556
                     /gene="ffh"
                     /locus_tag="VFP02_08255"
     CDS             159..1556
                     /gene="ffh"
                     /locus_tag="VFP02_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET9543059.1"
                     /translation="MFETLSDRFDGILKRLRGRGKLSEADVDEVLKEIRVALLEADVA
                     LSVARDLVERIRVRAIGAEVSESLSPAQQVIKIVNEELTVTLGGETFKLTYASKPPTV
                     VLLAGLQGSGKTTTSAKLARWFKQQGRNPLLIGADLQRPAAVEQLRTLGASIDVPVFS
                     EPKDPVKVAKAGMKEANRLGRDVVIVDTAGRLAIDQELMAQVRDISKQVKPDYTFLVV
                     DAMTGQDAVQTAQSFHQTLELDGVILTKLDGDARGGAALSVKEVVGRPIAFATTGEKI
                     EDLDLFHPDRLAGRILGMGDVLTLIEKAEEVYEAEQAEEDLARLMEGRFTLDDFLDQL
                     QQLKKMGPLSGIMGMLPGIPKEVKDAEIGDDELAPVEAIIRSMTVAERIDPKIIDGSR
                     RRRIADGSGTSPNQVNQLIKQFRQMQQMMRKMGAAPGGPGAKGRKGKQKKGGRTTTKG
                     AAPIPKMPLELPGLN"
     gene            1729..2073
                     /gene="rpsP"
                     /locus_tag="VFP02_08260"
     CDS             1729..2073
                     /gene="rpsP"
                     /locus_tag="VFP02_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET9543060.1"
                     /translation="MAVKLRLMRMGKKKQPTYRIVAADSRSPRDGRFIEVVGVYDPRA
                     EPSVIKVDNERAVHWLRHGAQPTERVQKLLQIAGAWDEFKGNAPASPPAAPAEEPAAE
                     ADGIPEGAEAAS"
     gene            2070..2417
                     /locus_tag="VFP02_08265"
     CDS             2070..2417
                     /locus_tag="VFP02_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET9543061.1"
                     /translation="MSDEGYDDTYEDDDANGSDDTGDDDYNRRDPNLIEGATAATVLE
                     YITKAVVDDPSAVEVDSVEGRRGTVSLEVQVADDDMGRVIGKRGRVANSIRTVVRAAA
                     ARDGVEVDVEFVE"
     gene            2431..2883
                     /gene="rimM"
                     /locus_tag="VFP02_08270"
     CDS             2431..2883
                     /gene="rimM"
                     /locus_tag="VFP02_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET9543062.1"
                     /translation="MLEVGRVAKAHGLRGEVVVALSTNRPERVATGTVLHTDRGPLTI
                     TASRPHQHRHIVRFAGVDTREAADALHGVILSAEPLEDPDALWVHELIGCVVVDQHGV
                     EHGAVTSVEVNPASDLLVLDGGGLVPLTFVTERADGRITVDVPDGLFD"
     gene            2889..3632
                     /gene="trmD"
                     /locus_tag="VFP02_08275"
     CDS             2889..3632
                     /gene="trmD"
                     /locus_tag="VFP02_08275"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET9543063.1"
                     /translation="MRIDVFTIFPAMVTTISGESLVGRAAEEGRLDLRVHDLRDATTD
                     VHRSVDDSPFGGGAGMVFMPGPVFDSVEAADPPRPLLLLGPGGRRLDQAYASELAALD
                     GFSLLCGRYEGVDERVHAHLVDGELSIGDYVLAGGEVAAMVVIEAVGRLVPGVLGNEA
                     SVEDESFSTGLLEYPHYTRPADFRGWEVPEVLRSGDHARVARWRRAQALARTIQRRPD
                     LVAARGGVTEEERSWLTEFGLGHLVDTLS"
     gene            3705..4052
                     /gene="rplS"
                     /locus_tag="VFP02_08280"
     CDS             3705..4052
                     /gene="rplS"
                     /locus_tag="VFP02_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET9543064.1"
                     /translation="MQPTDLIDQANLRDDVPDFAPGDTLKVHVRVVEGNRERVQVFEG
                     AVIGRQGSGVRETFKVRKLSFGVGVERTFPVHSPIIQKIEVVRKGDVRRAKLYYLRDR
                     VGKRARIKEKRDS"
     gene            4060..4404
                     /locus_tag="VFP02_08285"
     CDS             4060..4404
                     /locus_tag="VFP02_08285"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HET9543065.1"
                     /translation="MVSRRQQLGAEGEARVADHYEARGYTVLERNWRCRDGELDLVLR
                     SGRTLVICEVKTRSTAAFGTPAEAVTRAKQAKLRLLARHYLDTAPFRPRTIRFDVAAI
                     LAGQIEIIEAAF"
BASE COUNT          736 a         1658 c         1617 g          635 t
ORIGIN      
        1 cgggcggtgg cgaatgcctg gccgaagacg gcgcgggggt cgagctcggt cggggtctcg
       61 gtgttggtca tggaggcacc gtcgcacccc ttccggccag atcctggccg gaagagggga
      121 gttccatggc gcacgcccgt cgttaggatc gtcggctcat gttcgagacg ctttctgacc
      181 gcttcgacgg gatcctcaag cgcctgcggg gtcggggcaa gctctccgag gccgacgtcg
      241 acgaggtcct caaggagatc cgagtcgccc tcctcgaagc cgacgtcgcc ctgtcggtgg
      301 ctcgcgacct cgtcgagcgc atccgggtgc gggcgatcgg cgctgaggtc agcgagagcc
      361 tcagcccggc ccagcaggtc atcaagatcg tcaacgagga gctcaccgtc accctcggcg
      421 gggagacctt caagctcacc tacgccagca agcccccgac cgtcgtgctc ctggccggcc
      481 tccagggctc cggcaagacc accacctcgg ccaagctggc ccggtggttc aagcagcagg
      541 gccgcaaccc gctgctgatc ggtgccgacc tccagcgccc ggccgccgtc gagcagctgc
      601 gcaccctcgg cgcctcgatc gacgtccccg tcttcagcga gcccaaggac ccggtcaagg
      661 tggccaaggc gggcatgaag gaggccaacc gcctcgggcg cgacgtggtc atcgtcgaca
      721 ccgccggtcg cctggccatc gaccaggagc tgatggcgca ggtccgggac atctccaagc
      781 aggtcaagcc cgactacacg ttcctcgtcg tggacgccat gacggggcag gacgcggtcc
      841 agaccgccca gtccttccac cagaccctcg agttggacgg cgtcatcctc accaagctgg
      901 acggcgacgc ccgcggcggt gccgccctca gcgtcaagga ggtggtgggc cggcccatcg
      961 ccttcgccac cacgggcgag aagatcgagg acctcgacct cttccacccg gaccgcctgg
     1021 cggggcgcat cctgggcatg ggcgacgtcc tcaccctcat cgagaaggcc gaggaggtct
     1081 acgaagcgga gcaggccgag gaggacctcg ctcgcctgat ggagggtcgc ttcaccctcg
     1141 acgacttcct cgaccagctc cagcagctca agaagatggg cccgctcagc gggatcatgg
     1201 gcatgctccc cggcatcccc aaagaggtga aggacgccga gatcggtgac gacgaactgg
     1261 ccccggtcga agcgatcatc cggtcgatga ccgtcgccga gcgcatcgac ccgaagatca
     1321 tcgacgggtc ccggcgccgg cgcatcgccg acggcagcgg cacctcgccc aaccaggtca
     1381 accagctcat caagcagttc cggcagatgc agcagatgat gcgcaagatg ggagccgcgc
     1441 cgggtggtcc cggcgccaag ggccgcaagg gcaagcagaa gaagggcggc cgcaccacca
     1501 ccaagggcgc cgccccgatc cccaagatgc ccctggagct ccccggcctg aactgaggtc
     1561 gccgccgctc cctggagcgg cgccgggtgg gcaccgacgc ggccaacccg cgaccagccg
     1621 gcgggtcagg aaagatcctc ggtgagcggt agccttccgc gtcgctcacc ggggaccctt
     1681 tcgtccccgg tcgacagctc tcgaacagac cagcgaggaa ccagcactgt ggccgtgaag
     1741 ctccgcctga tgcggatggg caagaagaag cagccgacct accggatcgt ggcggccgac
     1801 agtcgctcgc cgcgcgacgg tcggttcatc gaagtcgtcg gcgtctacga cccccgggcc
     1861 gagccgtcgg tcatcaaggt cgacaacgag cgcgccgtgc actggctgcg ccacggggcc
     1921 cagcccaccg agcgggtgca gaagctcctg cagatcgccg gtgcgtggga cgagttcaag
     1981 ggcaacgccc ccgccagccc gcccgccgcc ccggcggagg agccggcggc cgaggccgac
     2041 ggcattcctg agggtgcgga ggctgcatca tgagcgacga gggctacgac gacacctacg
     2101 aggacgacga tgccaacggc agcgacgaca cgggtgacga cgactacaac cgcagggacc
     2161 ccaacctgat cgagggggcg accgcggcca cggtgctgga gtacatcacc aaggccgtcg
     2221 tcgacgatcc gagcgcggtc gaggtcgaca gcgtcgaagg tcggcggggc accgtgagcc
     2281 tcgaggtcca ggtggccgac gacgacatgg gtcgtgtgat cggcaagcgg ggccgcgtgg
     2341 ccaactccat ccgcaccgtg gtccgggcgg ccgcggcccg ggacggcgtc gaggtcgacg
     2401 tcgagttcgt cgagtagctc cgggccgccc ctgctcgagg tcgggcgggt ggccaaggcc
     2461 catggcctcc gcggggaggt cgtggtcgca ctgagcacca accggcccga gcgcgtggcc
     2521 acgggcaccg tgctccacac ggatcggggg ccgctgacca tcaccgcctc acgaccccac
     2581 cagcaccgtc acatcgtgcg cttcgccggc gtcgacaccc gcgaggcggc cgacgccctg
     2641 cacggggtga tcctctcggc cgagcccctg gaggacccgg atgcgctctg ggtccacgag
     2701 ctcatcgggt gcgtggtcgt cgaccagcac ggggtcgagc acggcgccgt cacctccgtc
     2761 gaggtgaacc cggccagtga cctgctggtg ctggacggtg gcggcttggt cccgctcacg
     2821 ttcgtgaccg agcgcgccga cgggcggatc accgtcgacg tgcccgacgg cctgttcgac
     2881 tgaccgccgt gcgcatcgac gtcttcacca tcttcccggc gatggtcacg accatcagcg
     2941 gcgagagcct cgtggggagg gccgccgagg agggccgtct cgacctgcgg gtccacgacc
     3001 tgcgggacgc cacgaccgac gtccaccgtt ctgtcgacga ctcccccttc ggcgggggcg
     3061 ccggcatggt cttcatgccg ggcccggtgt tcgactccgt cgaggcagcc gacccgcccc
     3121 gaccgctgct cctcctgggc cccggtggtc gacgcctcga tcaggcctac gcctccgagc
     3181 tggccgccct cgacggcttc tccctgctct gcgggcgcta cgagggcgtg gacgagcggg
     3241 tccacgccca cctcgtcgac ggcgagttgt ccatcggcga ctacgtgctg gccggcggag
     3301 aggtggcggc gatggtggtc atcgaagccg tgggccggct ggtgccgggc gtcctgggca
     3361 acgaggcgtc ggtcgaggac gagtccttct cgaccgggct cctcgagtac ccgcactaca
     3421 cccgtccggc cgacttccgc ggctgggagg tccccgaggt cctacgttcc ggcgaccacg
     3481 cccgcgtggc ccgctggcgc cgggcccagg cgctggcccg gacgatccag cgccgtccgg
     3541 acctcgtcgc cgcccggggc ggggtcaccg aggaggagcg ctcctggctg acggagttcg
     3601 ggctgggcca tctggtcgat acgctctcct gaccgttcga ccacttcccc cgggtccccg
     3661 aacgaccctc ccctgaatcc cgggcgcaca ggagcacgtg cacgatgcag ccaaccgatc
     3721 tcatcgacca ggccaatctg cgggatgacg tccccgattt cgccccgggc gacaccctga
     3781 aggtccatgt ccgggtcgtc gagggcaacc gcgagcgggt gcaggtcttc gaaggtgcgg
     3841 tcatcggccg ccagggcagc ggcgtgcggg agaccttcaa ggtccgcaag ctctccttcg
     3901 gggtcggcgt cgagcggacc ttcccggtcc actccccgat catccagaag atcgaggtcg
     3961 tccggaaggg cgacgtgcgc cgcgccaagc tctactacct gcgggatcgg gtcgggaagc
     4021 gggcccgcat caaggagaag cgcgacagct agcccggtca tggtctcccg gcgacagcag
     4081 ctgggggccg aaggggaggc gcgggtcgcc gaccactacg aagcccgtgg ctacaccgtg
     4141 ctggagcgga actggcgctg tcgcgacggc gagctggacc tggtcctgcg gagcggtcgc
     4201 accctggtca tctgcgaggt caagacgcgc agcaccgccg ccttcgggac gccggccgag
     4261 gccgtgaccc gggccaagca ggccaagctc cggctgctcg cccgccacta cctggacacc
     4321 gctccgttcc gccccaggac gatccgcttc gacgtcgccg ccatcctcgc cggccagatc
     4381 gagatcatcg aagccgcgtt ctgatccacc cagcgtgtcg ccggcgacgc acttgcgtac
     4441 ccagaactgt caggctggcc tgacatctgg ccgggaaggt tgcccgctac cggaccgttc
     4501 cggggcggcg gcgggcccgg tgctcccagc agcccttgtg ccagtgccgg cgcaggtcgg
     4561 gggccaggcg gggcaccgcg acgacgtgga aggtgcgggg cgggatgtcc tggttgcagc
     4621 cgggacagat gtaggccttg gtggcc
//
LOCUS       DASTFV010000449         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1673239, whole genome shotgun sequence.
ACCESSION   DASTFV010000449 DASTFV010000000
VERSION     DASTFV010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673239"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1772
                     /locus_tag="VFP02_08290"
     CDS             168..1772
                     /locus_tag="VFP02_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9543066.1"
                     /translation="MAPPTDAASINDRRWAVLAVMNLSLVIVVAGNSALNVALPSIVR
                     DLDATSSQLQWMVDAYALVFAGLLLPMGALGDRFGRKGMLQGGLSIFALGSLIAVFAT
                     EPWHLIATRSIMGVGAAMIMPATLSIITNVFPPQERSKAIAIWAGFAGAGAAIGPVLS
                     GFLLEHFWFGSVFLINIPIVAVALISGALLVPTSRDPRQRRLDPVGGLLSMVGLFALL
                     YAIIEGPGRGWTDPVTLGLFVASLVLGVIFVRWEHHTDEPMLPMEQFSDRRFSMGTGT
                     IGLTFFAMFGTFFVATQYLQFVLGYSPFQAGLATLPMAAMMVIFAPRSAGLAERFGRD
                     KVMAVGLTTVALGLFTMSFLTVDSPYIHLAIGLMLLGGGMAMTTAPATGAVMESMPLS
                     KAGVGSAVNDTSREVGGALGVAVLGSILSSGYRSALDLDLSAAPPEAAAAVREGVGSA
                     LAVAQQAPANIGPQIADAARTAFTDAMAPVFWFGAAVVLITAGLVLKVMPSRGDVVTH
                     EVGEDGYLAPTDAAARNESVQVPAGS"
     gene            1769..>2110
                     /locus_tag="VFP02_08295"
     CDS             1769..>2110
                     /locus_tag="VFP02_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9543067.1"
                     /translation="MSTAAEPAAGAGVDAGICDPRVARSRAAVLAATVDLLGEVGHAG
                     TTVEAIAERSGVAKTTIYRHWPSRAPLLIDAFHSSVEKGDHAPTGDVRADLLAIACGL
                     AAKLRDPQWSRI"
BASE COUNT          253 a          842 c          671 g          344 t
ORIGIN      
        1 ctgcaggccc tggccgccgg cgacgagcgc ttcgccccac tggcggagct cgctttggcc
       61 gcctgacccg cggctgtgcc cgatgtcacg gcgaagcccc ctggacagat tcgctacgct
      121 accgtagcgt agcgatagct cgcgatccgc cttcaggagg cacccccgtg gccccaccca
      181 ccgacgccgc atcgatcaac gaccgccgct gggcggtcct ggccgtgatg aacctcagcc
      241 tggtcatcgt cgtggccggc aactcggcct tgaacgtggc cctgccctcc atcgtccgcg
      301 acctcgacgc caccagctcc cagctgcagt ggatggtcga cgcctacgcc ctcgtcttcg
      361 ccggcctgct gctccccatg ggcgccctcg gggaccgctt cgggcgcaag ggcatgctcc
      421 agggcggcct ctcgatcttc gccctgggct cgctcatcgc ggtcttcgcc accgaaccgt
      481 ggcacctcat cgccacccgc tcgatcatgg gtgtcggggc ggcgatgatc atgcccgcca
      541 ccctctccat catcaccaac gtgttcccgc cgcaggagcg ctccaaggcc atcgccatct
      601 gggccgggtt cgccggggcc ggtgccgcca tcggcccggt gctcagcggc ttcctgctcg
      661 agcacttctg gttcggctcg gtcttcctga tcaacatccc gatcgtggcc gtggccctga
      721 tctccggcgc cctcctggtg ccgacctccc gggacccgcg ccagcgtcgc ctcgaccccg
      781 tcggcggcct gctctccatg gtcggcctct tcgccctcct ctacgccatc atcgagggcc
      841 ccgggcgggg ctggaccgat cccgtcaccc tcggcctctt cgtggcctcc ctggtcctcg
      901 gcgtgatctt cgtccgctgg gagcaccaca ccgacgagcc gatgctgccg atggagcagt
      961 tctcggaccg ccgcttctcc atgggcaccg ggaccatcgg gctgacgttc ttcgccatgt
     1021 tcggcacctt cttcgtggcc acccagtacc tgcagttcgt gctcggctac tcgcccttcc
     1081 aggcgggcct ggccacgctg ccgatggcgg cgatgatggt catcttcgcc ccccgcagcg
     1141 ccgggttggc cgagcgcttc ggacgggaca aggtcatggc cgtcggcctc accacggtcg
     1201 ccctcggcct cttcaccatg tcgttcctga ccgtcgactc gccgtacatc catctggcca
     1261 tcggcctgat gctcctgggc gggggcatgg ccatgaccac cgcaccggcg accggcgcgg
     1321 tcatggagtc catgcccctc tccaaggccg gtgtgggctc ggccgtcaac gacaccagcc
     1381 gtgaggtggg cggggccctc ggcgtcgccg tgctcggcag catcctgtcc tcgggctacc
     1441 ggtccgccct cgacctcgac ctgtcggcgg cgccgcccga ggccgcggcc gccgtccggg
     1501 aaggcgtcgg ctcggccctg gccgtggctc agcaggcccc ggcgaacatc gggccccaga
     1561 tcgccgacgc cgcccggacc gcgttcaccg acgccatggc cccggtcttc tggttcggcg
     1621 ccgccgtcgt cctgatcacc gccggactcg tcctgaaggt catgccgagc cggggtgacg
     1681 tcgtcaccca cgaggtgggc gaggacggct acctggcccc gaccgacgcc gcggcccgga
     1741 acgagtcggt ccaggtcccg gccggctcat gagcacggcc gccgagcccg cggccggcgc
     1801 cggtgtcgac gccggcatct gcgatccccg ggtcgcccgg tcccgggccg cggtcctcgc
     1861 cgccaccgtg gacctgctcg gcgaggtcgg ccacgccggt accactgtcg aggcgatcgc
     1921 cgagcgctcc ggcgtggcca agaccaccat ctaccggcac tggcccagtc gggccccgct
     1981 gctgatcgac gccttccaca gcagcgtcga gaagggtgac cacgccccga cgggggacgt
     2041 ccgggccgac ctcctggcca tcgcctgcgg cctggcggcc aagctccggg acccccaatg
     2101 gagccggatc
//
LOCUS       DASTFV010000450         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1683808, whole genome shotgun sequence.
ACCESSION   DASTFV010000450 DASTFV010000000
VERSION     DASTFV010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683808"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="VFP02_08300"
     CDS             complement(<1..1107)
                     /locus_tag="VFP02_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET9543068.1"
                     /translation="MDDEGQPYEPAEIEPRWQARWRDEGTYEVDNDDPRPKSYVLCMY
                     PYPSGAAHMGHVRNYTFGDLLVRYRTMNGQAVLSPIGFDSFGLPAENAAIKTGEHPRP
                     FTEAKIEELTSSLLRLGASYDWRRVIKSHDPSYIRWTQWIFLQFLKAGLAYRAEAPVN
                     WCPGCQTVLANEQVLADGTCERSGDLVQRKNLEQWFFRITDYAQQLLDDIDDLDWPER
                     VKTMQRNWIGRSEGATFHLPVAEREDLSLEVFTTRPDTSFGMTYAVVAPEHAIVAELT
                     TDDRRAEVEAFVAHVTGESELERLSSEGPLDKRGVFTGAHVVNPFTGDPIPVYLADYV
                     LATYGTGAIMAVPGQDQRDWDFAKAYDLPIVRTVL"
     gene            1227..1300
                     /locus_tag="VFP02_08305"
     tRNA            1227..1300
                     /locus_tag="VFP02_08305"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1261..1263,aa:Ala,seq:ggc)
     gene            complement(1340..1750)
                     /gene="rsfS"
                     /locus_tag="VFP02_08310"
     CDS             complement(1340..1750)
                     /gene="rsfS"
                     /locus_tag="VFP02_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET9543069.1"
                     /translation="MRRGVIPLAEVIEPEVLLAAVKAAAAAADDKNATDVLVLDVGDV
                     LSITGWFVIASGSNPRLVRTIAEEVEATLKAVHDLAPLRVEGLDGLRWVLLDYGDFVV
                     HVFHEEERDYYQLERLWADVPKVDWQTEATAEAG"
     gene            complement(1722..>2425)
                     /locus_tag="VFP02_08315"
     CDS             complement(1722..>2425)
                     /locus_tag="VFP02_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HET9543070.1"
                     /translation="EQADTVRLTRHEAFWTSWMSALDEAGESAVPGEGEVGVGGAVRG
                     LVGGPRNITTLPATPVPIPGVPLSESDLFRPNGLAIVATVPDLIPFPVGVGRLRTRLV
                     MGVEDQTQLLPDAAHTLVQAGAEISVIANDDEFDETETVVYFFRPSQREKARRLLNSL
                     GTGTLVKDTALSDNVDAVVVLGQDYIDLQGGTSPSTTAPAGVSVPTTVPIGGGVSGGI
                     PGGVTPGAPGGDPAG"
BASE COUNT          346 a          835 c          856 g          388 t
ORIGIN      
        1 cagcaccgtc cggacgatgg gcaggtcgta ggccttggcg aagtcccagt cccgctggtc
       61 ctggccgggc acggccatga tcgccccggt gccgtaggtg gccagcacgt agtcggccag
      121 gtagacgggg atcggatcgc ccgtgaacgg gttcaccacg tgggcgccgg tgaacacccc
      181 tcgcttgtcc aacgggccct ccgagctcag ccgctccagc tcgctctcac cggtcacatg
      241 ggccacgaag gcctcgacct cggcccgccg gtcgtcggtg gtcagctcgg ccacgatggc
      301 gtgctcgggc gcgaccacgg cgtaggtcat gccgaagctg gtgtcgggcc gggtggtgaa
      361 gacctccagg ctcaggtcct cccgctcggc gacgggcaag tggaaggtgg ccccctcgga
      421 gcgaccgatc cagttgcgct gcatggtctt gacccgctcg ggccagtcca gatcgtcgat
      481 gtcgtccagc agctgctggg cgtagtcggt tatccggaag aaccactgct ccaggttctt
      541 gcgctggacc aggtcgccgg agcgttcaca ggtgccgtca gccaggacct gctcgttggc
      601 caagacggtc tggcacccgg gacaccagtt caccggggcc tcggcccggt acgccagccc
      661 ggccttcagg aactgcagga agatccactg ggtccagcgg atgtaggacg ggtcgtggct
      721 cttgatcacc cggcgccagt cgtaggacgc ccccaggcgc aacagggacg aggtcagctc
      781 ctcgatcttg gcttcggtga acggccgcgg atgctcgccg gtcttgatgg cggcgttctc
      841 ggccggcagc ccgaagctgt cgaagccgat gggcgagagg acggcctggc cgttcatggt
      901 ccggtagcgg accagcaggt caccgaaggt gtagttgcgc acgtggccca tgtgggccgc
      961 cccgctcggg taggggtaca tgcacaagac gtaggacttc gggcgggggt cgtcgttgtc
     1021 gacctcgtag gtgccctcgt cccgccaacg ggcctgccag cggggctcga tctccgccgg
     1081 ctcgtacggc tggccctcgt cgtccatgcc gccgatcgta ccgacggccg acaacccctc
     1141 acgggccggt ttggccgtca cggcgcactc tgtccggcgc ccctgaggcg atccgatgtc
     1201 gccttggtaa tctcgcagcc gaatacgggg ctgtagctca gttggctaga gcacctgcat
     1261 ggcatgcagg gggtcggcgg ttcgagtccg ctcagctcca cagtttctcc tcgcccgtca
     1321 ggcgcagtgt tccggttgct cagccggctt cggcggtggc ctcggtctgc cagtcgacct
     1381 tggggacgtc ggcccagagg cgctcgagct ggtagtagtc ccgctcctcc tcgtggaaga
     1441 cgtggaccac gaagtcgccg tagtccagga gcacccagcg caggccgtcg aggccctcca
     1501 cccggagcgg ggccaggtcg tgcacggcct tcaacgtcgc ctcgacctct tcggcgatcg
     1561 tgcgcaccag ccgggggttc gagccggagg cgatgacgaa ccaccccgtg atggagagga
     1621 cgtcgccgac gtcgaggacc agcacgtccg tggcgttctt gtcgtcggcg gcggccgccg
     1681 ccgccttcac cgcggcgaga aggacctcgg gctcgatgac ctcagccagc gggatcaccc
     1741 cccggcgcac cgggcgtgac gccaccgggg atccccccgc tgaccccgcc accgatcggc
     1801 acggtggtgg gcaccgagac accggcgggg gccgtggtcg aggggctggt gccgccctgc
     1861 aggtcgatgt agtcctggcc gaggaccacg accgcgtcca cgttgtcgct gagcgcggtg
     1921 tccttgacca aggtgcccgt gcccaaggag ttgagcagcc gccgggcctt ctcccgttgg
     1981 ctcggtcgga agaagtagac gacggtctcg gtctcgtcga actcgtcgtc gttggcgatc
     2041 acgctgatct cggccccggc ctgcacgagc gtgtgcgccg cgtccggcag gagctgggtc
     2101 tggtcctcga cccccatgac cagccgggtg cgcaggcgcc cgacgccgac cgggaagggg
     2161 atgaggtcgg ggacggtggc cacgatggcc agcccgttgg gacggaagag gtcgctctca
     2221 ctcaacggga ccccggggat cggcaccggc gtcgcgggca gcgtcgttat gttgcggggc
     2281 ccgccgacga ggccgcgcac cgcaccgccg acgccgacct cgccctcgcc gggaaccgcc
     2341 gactcgcccg cctcgtccag ggcgctcatc cacgaggtcc agaaggcttc gtgccgggtc
     2401 aggcggacgg tgtcggcctg ctccg
//
LOCUS       DASTFV010000451         2566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1684325, whole genome shotgun sequence.
ACCESSION   DASTFV010000451 DASTFV010000000
VERSION     DASTFV010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684325"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /locus_tag="VFP02_08320"
     CDS             complement(<1..1403)
                     /locus_tag="VFP02_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9543071.1"
                     /translation="MAGVAGPTRLFIGGEFVDAADGATIPVTSPHDGSLLAEVAEARE
                     ADVDRAVAAARSAFGAWSATPAAERGRLLLKLADAVEERAAELARLESTDTGHPIRDS
                     TRLDVPRTAATFRYFGGMADKLEGSVVPVERGFLNYVVREPVGVVGQIVPWNFPLMFC
                     SWKMGPALAAGNTVVMKPAELTPLSSLRMAELMAEVGFPPGVVNMVPGYGHVAGAALA
                     AHRGVQKVSFTGSTEVGRRIVEASAGNLKRLQLELGGKGANIVFADADVAAAVNGSAF
                     AIFHNQGQACIAGSRLILEEPIAEEFQERFLALARSIRLGDPLDEATEMGPLTSPEHR
                     DRVLAYVKVARDEGGEVLCGGRAPDDPALARGCYVEPTVVRADPGARVNREEVFGPFV
                     TVTTFSGEAEALELANSVGYGLGGGLWTRDLARAHRVARAIRSGMVWVNSYKRVSPGS
                     PFGGVGDSGYGREMGFEA"
     gene            complement(1412..2407)
                     /locus_tag="VFP02_08325"
     CDS             complement(1412..2407)
                     /locus_tag="VFP02_08325"
                     /inference="COORDINATES: protein motif:HMM:NF036733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543072.1"
                     /translation="MATTEAEPTVDVRSGDPNEAGYRPFELGEFTFRRDDYFVYIEWP
                     TGSHVMSADAFLKALQRDVAWDFFYGIVNFDGVIGTVNHYGTVDLFAGRYNDGYRKAE
                     LDHLENMPTPAIRETFKAMLDDWTNETFDPFASPDETGSAFGPKNGDNRKAITRHRVA
                     AARMVGVKGDEQIRSDDSGFPVNRHFADVPQDEPEIRAEAGFEDELASFNLFAYLSRS
                     DVTWNPSVVSVCKDSLFCPTTEEYILPIIHGNDRVEWFLQLSDEIYWDVEDRDSGQIR
                     SKVVMKPGDVAAMPADIRHQGYAPKRSMLLVWENAAGDLPELISSGKLPTYPVEL"
BASE COUNT          348 a          901 c          935 g          382 t
ORIGIN      
        1 gcctcgaagc ccatctcgcg cccgtagccg gagtcgccga ccccgccgaa cggcgagccc
       61 gggctgaccc gcttgtagct gttgacccag accatccccg accggatcgc ccgcgccacc
      121 cggtgggccc tggccaggtc gcgcgtccag agcccgcccc cgaggccgta gccgacgctg
      181 ttggccagct ccagggcctc ggcctcgccg gagaaggtgg tcacggtcac gaagggcccg
      241 aacacctcct cgcggttgac ccgcgccccg gggtcggccc tgaccacggt cggctccacg
      301 tagcagcccc tggccagcgc cgggtcgtcg ggggcgcggc cgccgcacag gacctcgccg
      361 ccctcgtcgc gggccacctt cacataggcc agcacccggt cgcggtgctc gggggaggtg
      421 agcgggccca tctcggtcgc ctcgtccagc gggtcgccca gccggatgct ccgggccagc
      481 gccaggaagc gctcctggaa ctcctcggcg atcggctcct ccaggatcag ccgggagccg
      541 gcgatgcagg cctgaccctg gttgtggaag atggcgaagg ccgacccgtt gaccgccgcg
      601 gccacatcgg cgtcggcgaa cacgatgttg gcccccttgc cgcccagctc cagctggagg
      661 cgcttgaggt tgccggccga ggcctcgacg atccgccggc cgacctcggt ggagccggtg
      721 aacgagacct tctgcacgcc ccggtgggcg gccagggcgg ccccggcgac gtggccgtag
      781 cccggcacca tgttgaccac gccgggcggg aagccgacct cggccatcag ctcggccatg
      841 cgcagcgagg acaacggggt cagctcggcc ggcttcatca ccaccgtgtt gccggcggcc
      901 agggccggcc ccatcttcca gctgcagaac atcagcggga agttccaggg cacgatctgg
      961 ccgaccaccc cgaccggctc gcggacgacg tagttgagga agccccgttc gaccgggacc
     1021 acactgccct cgagcttgtc ggccatgccg ccgaagtagc ggaaggtggc ggcggtgcgg
     1081 ggcacgtcca ggcgggtcga gtcccggatc gggtggccgg tgtcggtcga ctccagccgg
     1141 gccagctcgg cggcccgctc ctcgacggcg tcggccagct tcagcagcag ccgcccccgc
     1201 tcggcggccg gggtggcgct ccaggcgccg aaggcggacc gggcggccgc caccgcccgg
     1261 tcgacgtcgg cctccctggc ctcggccacc tcggccagca ggctgccgtc gtgcgggctg
     1321 gtgaccggaa tggtcgcccc gtcggcggcg tcgacgaact cgcccccgat gaacagccgg
     1381 gtcggcccgg ccacgccggc cacggcggcc gctagagctc gaccgggtac gtgggcagct
     1441 tgccgctgga gatgagctcg ggcaggtcgc cggcggcgtt ctcccagacc agcagcatcg
     1501 accgcttggg ggcgtagccc tggtggcgga tgtcggccgg catggcggcg acgtcgcccg
     1561 gcttcatgac caccttggag cggatctggc cggagtcgcg gtcctcgacg tcccagtaga
     1621 tctcgtccga cagctgcagg aaccactcca cccggtcgtt gccgtggatg atcggcagga
     1681 tgtactcctc ggtggtgggg cagaacaggc tgtccttgca gaccgagacc accgacgggt
     1741 tccaggtcac gtccgagcgc gacaggtagg cgaacaggtt gaaggaggcc agctcgtcct
     1801 cgaagcccgc ctcggcccgg atctccggct cgtcctgggg gacgtcggcg aagtgccggt
     1861 tgaccgggaa gccgctgtcg tcgctgcgga tctgctcgtc gcccttgacc ccgaccatgc
     1921 gcgcggccgc gacccggtgg cgggtgatgg ccttgcggtt gtcgccgttc ttggggccga
     1981 aggccgagcc ggtctcgtcc gggctggcga acgggtcgaa ggtctcgttg gtccagtcgt
     2041 ccagcatcgc cttgaaggtc tcgcggatgg ccggggtcgg catgttctcg aggtggtcga
     2101 gctcggcctt gcggtagccg tcgttgtagc ggccggcgaa caggtcgacc gtgccgtagt
     2161 ggttgacggt gccgatgacc ccgtcgaagt tgacgatgcc gtagaagaag tcccaggcca
     2221 cgtcccgctg cagcgccttg aggaaggcgt cggcgctcat cacgtggctg ccggtcggcc
     2281 attcgatgta gacgaagtag tcgtcgcggc ggaacgtgaa ctcgcccagc tcgaaggggc
     2341 ggtagccggc ctcgttgggg tctccggagc ggacgtcgac ggtgggttcg gcctcggtcg
     2401 ttgccacggt gctacctcct gccttgcgcc cctacggggt ggtctgcacg atctcggccc
     2461 acttgaagac ggtgtcgggg ccctcgatgg tctgcatcag gatcacgccc ggccggtcgg
     2521 cgtggaagcg gtaggcggcg ccggagggga gcagggtcat gtggcc
//
LOCUS       DASTFV010000452         3254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1684767, whole genome shotgun sequence.
ACCESSION   DASTFV010000452 DASTFV010000000
VERSION     DASTFV010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684767"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFP02_08330"
     CDS             <1..378
                     /locus_tag="VFP02_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9543073.1"
                     /translation="KPFAHVVLVARLRALVRRGRPARPVVLTAGDLRLDPGSRRVWRG
                     EDEIALTARETSLLEFLLQEAGTVVSKREILDHVWDDDFDGDPNIVEVYVRHLRNKLD
                     RPFDRDSIHTVRGSGYRLDPRGG"
     gene            378..1709
                     /locus_tag="VFP02_08335"
     CDS             378..1709
                     /locus_tag="VFP02_08335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9543074.1"
                     /translation="MRSLRTRAVIGAVTVVGVALLIGSVSMVLLVRSSLTGNVREAAE
                     LRADDVATVLEAGGESAADLTVEDDEDALIQVIGTEGDVVASSPNVDGLPPVADLGDE
                     QSAVLDHVSVGGDRFVAVAVTADTPEGEVTVLVARALDDADEATWALVEVLAIGVPLL
                     LVLVGLVGWALVGRALAPVAAMIGAADEITATDLDRRLPEPEGADEIARLGTTLNAML
                     DRLADSRDRMSAFVADAAHELRSPVSSLRQHAEVALEHPDRTEVTDLAEVVRAESLRQ
                     QRLVDDLLLLARVDEGAAARRDRAVDLDDAVAVAVARLRPVSPVVIDTSGVSGGQVIG
                     DPSGLDRLVGNLLENAARHANARVEVTLAERDDVVAFTVDDDGPGIPGPDQERIFERF
                     VRLDESRSRDEGGSGLGLAIVAAVAAAHGATVAVGDSPLGGARFRVVFPGP"
     gene            1751..2083
                     /locus_tag="VFP02_08340"
     CDS             1751..2083
                     /locus_tag="VFP02_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543075.1"
                     /translation="MTITTKTKVLIAGVAAVTAAGAGVGIAAAGGGDDSEGPITGSEL
                     DRASAAALAETGEGRVTETEVGDEDSFYEVEVTLDDGSQVDVQLDRDFQVVSSSTDHE
                     DDAGDGGD"
     gene            complement(2140..2892)
                     /locus_tag="VFP02_08345"
     CDS             complement(2140..2892)
                     /locus_tag="VFP02_08345"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:NF002198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HET9543076.1"
                     /translation="MLIGGHVEPDDPLGTAVAEDADCVQIFLGDPQGWKKPPPREDAA
                     ALKASDTIVYVHAPYIVNVASANNRIRIPSRKILQQTVDAAAEIDAAGVIVHGGHVTA
                     DDSEAEGFLRWVKAMDQLDMKVPVLLENTAGGDHAMARHFDTIAGLWDHIGDSGIGFC
                     LDTCHAHAAGEELISAAERILAITGRIDLVHCNDSKDGHGSGRDRHEHLGQGNCDPAA
                     LLETVKVADAPVICETGADARKDDIAWLRDQL"
     gene            complement(2903..>3254)
                     /locus_tag="VFP02_08350"
     CDS             complement(2903..>3254)
                     /locus_tag="VFP02_08350"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9543077.1"
                     /translation="DPLPDHGPFDAIVSSFAIHHVADERKRTLYVECFDRLRPGGAFL
                     NLEHVASPTRGLHEAFLTELGIDPADDDPSNLLAPVDDQLRWLRDAGFTDVDCHWKWR
                     ELALLVGVRPAADR"
BASE COUNT          440 a         1151 c         1181 g          482 t
ORIGIN      
        1 aagccgttcg cccatgtggt gctcgtggcc cgactccggg cgctggtccg gcggggtcga
       61 cccgcccgac ccgtcgtgct caccgcgggt gacctccgcc tcgaccccgg ctcgcgacgg
      121 gtctggcggg gggaggatga gatcgcgctg acagcgcggg agacctccct gctcgagttc
      181 ctgctgcaag aggccggcac ggtggtgtcg aagcgagaga tcctcgacca cgtctgggac
      241 gacgacttcg acggcgaccc caacatcgtc gaggtctacg tccgccacct gcgcaacaag
      301 ctggaccggc ccttcgatcg ggacagcatc cacaccgtca ggggctcggg ctaccggctc
      361 gacccgcgcg gcggctgatg cgctccctcc gcacccgggc cgtcatcggc gcggtgacgg
      421 tcgtgggcgt cgccctcctg atcggctcgg tgagcatggt cctgctcgtt cggtcgtcgt
      481 tgaccggcaa cgtgcgggag gcggccgagc tccgggcgga cgacgtggcc accgtgctcg
      541 aagccggtgg ggaatcggcc gccgacctga ccgtcgagga cgacgaggac gccctcatcc
      601 aggtgatcgg gaccgagggt gatgtcgtcg cctcgagccc caacgtcgac gggctccctc
      661 ccgtcgccga cttgggcgac gagcagtcgg ctgtcctgga ccacgtctcc gtgggcggcg
      721 accgcttcgt cgccgtggcc gtgaccgcgg acacgcccga gggtgaggtg accgtgctcg
      781 tggcccgtgc cctcgacgac gccgacgaag cgacctgggc cctggtggag gtgctggcca
      841 tcggtgtgcc cctcctgctg gtcctggtcg gccttgtcgg ctgggcgctg gtcggccggg
      901 ccctggcccc ggtcgccgcc atgatcggtg cggctgatga gatcacggcc accgatctgg
      961 accggcgcct gcccgagccc gagggagctg atgagatcgc ccggctgggc acgacgctca
     1021 acgccatgct cgatcgcctc gccgactcac gagaccggat gtcggccttc gtggccgacg
     1081 cggcccacga actgcgctcc ccggtgagct cactgcgcca gcacgccgag gtggctctcg
     1141 agcaccccga ccggaccgag gtcacggacc tggccgaggt ggtccgggcc gagagcctgc
     1201 gccagcaacg cctcgtcgac gacctgctct tgttggcccg cgtcgacgag ggggccgcgg
     1261 ctcgtcggga tcgggcggtc gatctcgatg acgcggtggc cgtcgcggtc gcccggctcc
     1321 gccccgtcag cccggtcgtc atcgacacgt cgggggtgtc ggggggccag gtcatcggcg
     1381 acccgtccgg gctcgaccga ctggtgggca acctgttgga gaacgccgcc cgtcacgcca
     1441 acgcccgggt cgaggtcacc ctcgccgaac gcgacgacgt cgtcgccttc acggtggacg
     1501 acgacggccc gggcatcccg ggcccagacc aggagcggat cttcgagcgc ttcgtgcgcc
     1561 tcgacgaatc ccgatcccgc gacgagggtg gcagcgggtt gggcctggcc atcgttgcgg
     1621 cggtcgccgc cgcccacggg gccaccgtcg ccgtcgggga cagcccgctc ggcggcgccc
     1681 ggttccgggt cgtgttcccc gggccctgac gttcaggtcc cgctcagcgg ccgattcgta
     1741 ctgtcgtccc atgaccatca ccacgaagac caaggtcctg atcgccggtg tcgccgcggt
     1801 cacggccgcc ggtgcgggcg tgggcatcgc cgccgccgga ggcggcgacg acagcgaggg
     1861 ccccatcacc gggtccgagc tcgaccgggc gtcggccgcc gccctggcgg agaccggcga
     1921 gggccgcgtc accgagaccg aagtcggcga cgaggacagc ttctacgagg tcgaggtcac
     1981 cctcgacgac ggcagccagg tcgacgtcca gctcgaccgg gacttccagg tcgtgagctc
     2041 gtcgaccgac cacgaggacg acgccggcga cggcggggac tgatcaccga ccgttccgcc
     2101 cgcggccctc gaccctccag atcgggggcc cgtggcgcgt cagagctggt cgcggagcca
     2161 ggcgatgtcg tccttgcggg cgtcggcgcc ggtctcacag atgaccggcg catcggccac
     2221 cttcaccgtc tccagcaggg ccgcgggatc acagttgccc tggccgaggt gctcgtggcg
     2281 gtcccggccg gagccgtggc cgtccttgga gtcgttgcag tggaccaggt cgatccggcc
     2341 ggtgatggcc aggatccgct cggccgcgga gatgagctcc tcaccggccg cgtgcgcgtg
     2401 gcacgtgtcc agacagaagc cgatgccgct gtcgccgatg tggtcccaga gcccggcgat
     2461 ggtgtcgaag tggcgggcca tcgcgtgatc gccccccgcg gtgttctcga gcagcacggg
     2521 caccttcatg tccagctggt ccatggcctt gacccagcgc aggaagccct cggcctccga
     2581 gtcgtcggcg gtgacatggc caccgtggac gatcaccccg gccgcatcga tctcggccgc
     2641 ggcgtcgacg gtctgctgca ggatcttgcg ggacgggatc cggatccggt tgttggccga
     2701 ggccacgttg acgatgtacg gggcgtggac gtagacgatc gtgtccgacg ccttgagcgc
     2761 cgccgcgtcc tctcggggcg ggggcttctt ccagccctgc gggtcgccaa ggaagatctg
     2821 gacacagtcg gcgtcctccg ccaccgcggt ccccagaggg tcgtcgggct cgacatggcc
     2881 accgatcagc atgatgctga ccctaccgat ccgcggccgg gcggacgccg acgaggaggg
     2941 ccagctcccg ccacttccag tggcagtcga cgtccgtgaa accggcgtcc cgcagccacc
     3001 tgagctggtc gtcgaccgga gccaggaggt tggacgggtc gtcgtcggcg gggtcgatcc
     3061 ccaactcggt caggaaggcc tcgtgcaggc cccgagtcgg cgaggccacg tgctccaggt
     3121 tcaggaaggc gccgccgggc cgaagccggt cgaagcactc cacgtaaagg gtgcgcttgc
     3181 gctcgtcggc cacgtggtgg atggcgaagc tggacacgat cgcgtcgaac gggccgtggt
     3241 cggggagcgg gtcg
//
LOCUS       DASTFV010000453         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1687731, whole genome shotgun sequence.
ACCESSION   DASTFV010000453 DASTFV010000000
VERSION     DASTFV010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687731"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1743
                     /locus_tag="VFP02_08355"
     CDS             <1..1743
                     /locus_tag="VFP02_08355"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HET9543078.1"
                     /translation="ERSLDEGDGHSGHRLVMPVADGQEIVVEKIVPIYTSRDQGIYEP
                     GDHAVRHVAWASGYDDLAVSNTRAWAHVWRRTDIEIEGSESLARGLMLQLYQLHQVIS
                     KHTTTLDVGIPARGLVGEAYRGHIFWDELFVFPYVNLRVPEHARALLMYRFRRLDEAR
                     RRAREAGFEGALFPWQSASSGREETPQAHLNPRSGHWLPDHSRLQRHVNSAIAHNVVR
                     YLDATDDRDFLSFSGAELMVEIARFWASAATYDHIEDRYEIRGVVGPDEYHEALPDAD
                     HPGVDNNAYTNVMAAWVLWKSGEVLDRLDDDRREEIVAKLAITAKERERWDEISRRMR
                     VCFHDGVISQFEGYERLHELDWDAYRARYGNIQRLDRILEAEGDTANRYRLSKQADVL
                     MLLYLFSPREVGELFDRLGYVVDEACLDRTIDYYLTRTSNGSTLSQVVHAWVVARRDL
                     PQSFDALCDALRSDLEDTQGGTTAEGVHIGAMAGTVDLLERCYTGLTIHDDAVWFDPA
                     VPPELGPYSFNLRFRGNWLDVRVSAAGIELKAHGHNRPVPIGFDGEVSDLAPGERRVL
                     ARTFDPGIGTDGMV"
     gene            1751..2170
                     /locus_tag="VFP02_08360"
     CDS             1751..2170
                     /locus_tag="VFP02_08360"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9543079.1"
                     /translation="MESTPRGRRLVAIAEEECWQLVSTRRWGRLLVVVANHPELFPVD
                     HLVDGRSLLVRTEEGTKLRAALGARVGFEVDEVDDDARLGWTVMLAGYANEVFDTREL
                     ELAEVDADEAVWTGDRVHWLRIVPFKVSGRRLVALAS"
     gene            2284..>2536
                     /locus_tag="VFP02_08365"
     CDS             2284..>2536
                     /locus_tag="VFP02_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9543080.1"
                     /translation="MTADSSPPSEQDGVPWWREAVIYEIYVRSFADADGDGLGDLAGI
                     RSRLPALVDLGVDAIWLTPFYPSPQADGGYDVADPCAVDP"
BASE COUNT          391 a          906 c          861 g          378 t
ORIGIN      
        1 gaacgctccc tcgacgaggg cgacgggcac agcggtcacc gtctggtcat gcccgtcgcc
       61 gacggtcagg agatcgtggt cgagaagatc gtccccatct acacctcccg ggaccagggc
      121 atctacgagc cgggcgacca cgccgtccgc cacgtcgcct gggcctcggg ctacgacgac
      181 ctggcggtca gcaacacccg ggcctgggcc cacgtctggc gccgcaccga catcgaaatc
      241 gaagggtccg aatcactggc ccggggcctg atgctgcagt tgtaccagtt gcaccaggtc
      301 atctcgaagc acacgacgac cctggacgtg ggcatacccg ctcgcgggct ggtcggcgag
      361 gcctaccggg gccacatctt ctgggacgag ctcttcgtgt tcccctacgt gaacctccgg
      421 gtgcccgagc acgcccgggc gctgctgatg taccgcttcc gccgcctcga cgaggcccgg
      481 cgccgcgccc gggaggccgg cttcgagggc gccctcttcc cgtggcagag cgcatcgagt
      541 ggccgcgagg agactcccca ggcccatctc aacccccgct cggggcactg gctgcccgac
      601 cactcccggc tccagcggca cgtcaactcc gccatcgccc acaacgtcgt gcgctacctc
      661 gacgccaccg atgaccggga cttcctctcc ttcagcggcg cggagctcat ggtggagatc
      721 gcccgcttct gggccagcgc ggcgacctac gaccacatcg aggaccggta cgagatccgt
      781 ggcgtggtcg gtcctgacga gtatcacgag gccctgcccg atgccgacca ccccggcgtc
      841 gacaacaacg cctacaccaa cgtgatggcg gcctgggtcc tctggaagag cggcgaggtt
      901 ctcgatcgcc tggacgacga ccgccgcgag gagatcgtgg ccaagctggc gatcaccgcc
      961 aaggagcggg agcgctggga cgagatcagt cgccggatgc gggtctgctt ccatgacggc
     1021 gtcatcagcc agttcgaggg ctatgagcgc ctccacgagc tggactggga cgcgtaccgg
     1081 gcccgctacg gcaacattca acgcctcgac cggatcctcg aggccgaggg cgacacggcc
     1141 aaccgctacc gcctgtccaa gcaggccgac gtgctgatgc tcctctacct gttctcgccc
     1201 agggaggtgg gcgagctgtt cgaccggctg ggctacgtgg tcgacgaggc atgcctcgac
     1261 cgcacgatcg actactacct gacccggacc tcgaacgggt cgaccctcag ccaggtggtg
     1321 cacgcatggg tggtggcccg acgtgacctc ccgcagtcct tcgacgccct ctgtgacgcc
     1381 cttcggagcg atctcgagga cacccagggc ggaaccaccg ccgaaggcgt ccacatcggc
     1441 gccatggccg ggaccgtcga cctcctcgag cgttgctaca cggggttgac gatccacgac
     1501 gacgcggtct ggttcgaccc ggccgtcccg ccggagctcg ggccctactc gttcaacctc
     1561 cgcttccggg gcaactggct cgacgtgcgg gtctcggccg cgggcataga gctcaaggcc
     1621 catggccaca accggccggt gcccatcggc ttcgacggcg aggtgagcga cctcgcgccg
     1681 ggtgagcgcc gggtactggc gaggaccttc gaccctggaa tcggcaccga cggcatggtc
     1741 tgatcggtgg atggaaagca ccccccgggg ccgccgcttg gtggcgatcg ccgaggagga
     1801 gtgctggcag ctcgtgtcca cgcggcgctg gggccgcctc ctcgtcgtgg tggcgaacca
     1861 tcccgagctg ttccccgtcg atcacctggt cgacggtcgc agcttgctcg tgcgcaccga
     1921 agaggggacc aagctgcggg ccgcgctcgg ggcccgagtc ggcttcgagg tcgacgaggt
     1981 cgacgacgac gcccggctgg gctggaccgt gatgctcgcc ggctacgcca atgaggtctt
     2041 cgacacccgt gagctcgagc tcgccgaggt cgacgccgac gaggccgtgt ggaccggtga
     2101 ccgggtccac tggctgcgca tcgtgccctt caaggtcagc gggcgacgcc tggtcgcgct
     2161 tgcttcctga cccgctcgag gtcaaccgag tcggaccgcg aagagcgcgg gcaggggcgt
     2221 cggcgacgcc cgacttgccg gtcatcggac ggaggccttg accggcccgc gaggatcgca
     2281 ggaatgaccg ccgactcgag cccgccgagc gagcaggacg gggtcccgtg gtggcgcgag
     2341 gcggtcatct acgagatcta cgtccgcagc ttcgccgacg ccgacggcga cgggctcggt
     2401 gatctggccg gcatccggtc ccggctcccg gccctggtgg acctcggggt ggacgccatc
     2461 tggctgacgc ccttctaccc gtcgccgcag gccgacgggg gctacgacgt cgccgacccg
     2521 tgcgccgttg acccgc
//
LOCUS       DASTFV010000454         3566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1691388, whole genome shotgun sequence.
ACCESSION   DASTFV010000454 DASTFV010000000
VERSION     DASTFV010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691388"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFP02_08370"
     CDS             <1..249
                     /locus_tag="VFP02_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543081.1"
                     /translation="KDLVERSRKSSEALSERIRSEVRDQLVKIQPATQADVDALAKRV
                     AAVEKATKKPAARKPAARKPAARKPAAKKPAAKKPAAR"
     gene            250..999
                     /locus_tag="VFP02_08375"
     CDS             250..999
                     /locus_tag="VFP02_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET9543082.1"
                     /translation="MAARRRLDLDLVRRGLVDSRQQAHALIEAGKVTVGGAIATKPAR
                     MVDPGEPVLILGDPPRFVSRGGEKLDAALDGFTVAVEGCRALDAGSSTGGFTDCLLQR
                     GCASVIAVDVGRGQLHPRMREDPRVTVRERTDIRTIDLDELGGPVDLVVGDLSFISLR
                     TVLDALLALVRPGGDLVLLVKPQFEVGRAEASRGKGVIRDPEVRAAALADVRDALTGR
                     GAVIMGEMTSPLKGGAGNVEFLVHARAPEAG"
     gene            999..1856
                     /locus_tag="VFP02_08380"
     CDS             999..1856
                     /locus_tag="VFP02_08380"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET9543083.1"
                     /translation="MAKVGLVLHHQREEAATVTRDVASWLGDRGHEVELPEPDAHLAG
                     LEDRGVPEDWFGEGLDLVVSLGGDGTMLRTVSLVAAHDVPVLGVNFGQLGYLTEIDPA
                     DLSRSLERFFADDYRIEERMLLEVRLAQGAAGDKRGWVALNEAVLERTAAGHTVRLGV
                     DLDGEFFTTYAADALIVATPTGSTAYSLSARGPIVAPDHRALLLTPVSPHMLFDRTLV
                     LNADEEVRLEVQGHRPATLSVDGQPVVDLVDGDAIECVASSHAALLVTFGARDFHQIL
                     KAKFGLADR"
     gene            1864..3453
                     /gene="recN"
                     /locus_tag="VFP02_08385"
     CDS             1864..3453
                     /gene="recN"
                     /locus_tag="VFP02_08385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET9543084.1"
                     /translation="MLAELAVENLGVIDRLQLVLGPGMTALTGETGAGKTLLVEAVDL
                     LVGGRADPVLVGPAGEEAIVEGRFVSGHDEIVLRRVIPSDGRSRAYVDGRLATASALS
                     EAGKLLVDLHGQHAHQSLLQGPAQRAALDRFGDIDLSGLEAARAELREIEVALADLGG
                     DDRARARELDLLRFQVAEIDAAAITGPDEDQELEAEGDRLGDAVAHREAAEGAADLLL
                     GDDAARDRLAAAGATLADRAPFKDLAGRLVGLQADLEDVAAALRDAAETIEPDPERLA
                     VVRERRHLLSELRRKYGDTLGEVLEFRAETAARLSELESHEERAAALDERRARAEKVL
                     GEAAAKVGRARRAAAPALADAAVTRLQELAMPNARLAIDVGADPGDDVTIQFSANPGS
                     APQPLAKVASGGELARTMLALRLVTTEAPPTLVFDEVDAGIGGTTALALAAALAELGD
                     EHQVLVVTHLPQVAAFADAQVTIEKQVTGERTATQARPVDGDARIVELSRMLSGQPDS
                     ATARQHADELLGEAAARRAIR"
BASE COUNT          476 a         1301 c         1315 g          474 t
ORIGIN      
        1 aaggacctgg tcgagcgctc ccgcaagtcg agcgaggcgc tgtccgagcg gatccgcagc
       61 gaggtcaggg accagctggt caagatccag cccgcgacac aagccgatgt ggatgccctg
      121 gccaagcgcg tcgccgccgt cgagaaggcg acgaagaagc ccgcggccag gaagcccgcg
      181 gccaggaagc cggccgcccg caagccggcg gccaagaagc ccgcggccaa gaagccggcg
      241 gccaggtagc tggctgcccg ccggcggctc gacctcgacc tcgtccggcg gggcctggtc
      301 gactcccgcc agcaggccca tgccctgatc gaggcgggca aggtgacggt ggggggcgcc
      361 atcgccacca agccggcccg catggtcgac ccgggtgagc cggtgctcat cctcggtgac
      421 cctccccgtt tcgtcagccg cggcggcgag aagctcgacg ccgcgctgga cgggttcact
      481 gtggccgtcg agggctgccg ggccctggat gccgggtcct cgacgggcgg tttcaccgat
      541 tgcctgctcc agcggggctg tgcctccgtc attgcggtgg acgtgggccg gggccagctc
      601 caccccagga tgcgggagga ccctcgggtc accgttcggg agcgaacgga catccggacc
      661 atcgacctcg acgaactggg tgggccggtc gacctggtcg tcggtgacct ctcgttcatc
      721 tcgctgcgga ccgtgctcga cgccctgctc gctctggtcc gcccgggagg cgatctggtc
      781 ctactggtga agccccagtt cgaggtcggg cgggccgagg ccagccgggg caagggggtc
      841 atccgggacc cggaggtgcg ggcggcggcg ctggccgacg ttcgagacgc gctcaccggc
      901 cgcggagccg tcatcatggg ggagatgacc tctcccctga agggtggcgc cgggaacgtc
      961 gagttcctgg tccacgcccg cgcccccgag gccggctgat ggcgaaggtc gggttggtcc
     1021 tgcatcacca gcgggaggag gcggccaccg tcacccggga cgtcgcctcc tggctcgggg
     1081 atcggggtca cgaggtcgaa ctgcccgagc ccgacgccca cctggccggg ctcgaagacc
     1141 gcggcgtacc cgaggactgg ttcggcgagg gcctggatct cgtggtgagc ctcggtggcg
     1201 acggcacgat gttgcgcacg gtcagcctgg tggcggccca cgacgtgccc gtcctcggcg
     1261 tgaacttcgg ccagttgggc tacctcacgg agatcgatcc cgccgacctg tcccgctccc
     1321 tggagcgctt cttcgccgac gactaccgca tcgaggagcg catgctgctg gaggtgcgcc
     1381 tggcccaggg ggcggccggc gacaagcgcg gctgggtcgc gctcaacgag gccgtactcg
     1441 agcggacggc cgcgggccac accgtgcggc tcggggtgga cctcgacggt gagttcttca
     1501 ccacgtacgc cgccgacgcc ctgatcgtgg ccacccccac ggggtcgacc gcgtactcac
     1561 tgtcggcccg gggcccgatc gtggcccccg accaccgggc cctcctgctc accccggtct
     1621 cgccgcacat gctcttcgac cggacgctgg tgctcaacgc cgacgaggag gtccgcctcg
     1681 aggtccaggg tcaccgcccg gccacgctct ccgtcgacgg ccagccggtg gtcgacctgg
     1741 tcgacggtga cgccatcgag tgcgtggcct cgagccatgc cgccctgctg gtcaccttcg
     1801 gggcccggga cttccaccag atcctgaagg ccaagttcgg cctggccgac cggtgagcgg
     1861 ctgatgctgg ctgagctggc cgtcgagaac ctgggtgtca tcgaccgcct tcagctggtg
     1921 ctggggcccg gcatgactgc cctcaccggc gagaccggag caggcaagac gctgctggtc
     1981 gaggccgtcg accttctcgt cggtggccgg gccgatcccg tgctggtggg cccggccggt
     2041 gaggaggcga tcgtcgaagg tcgcttcgtc tcgggccacg acgagatcgt gctgcgccgg
     2101 gtcattccgt ccgacgggcg gagccgggcg tacgtcgacg gtcggctggc caccgcctcc
     2161 gccctcagcg aggcgggaaa gctcctggtc gacctgcacg gccagcacgc ccaccagtcc
     2221 ctgctccagg gcccggccca gcgggccgcc ttggaccgct tcggcgacat cgatctgtcg
     2281 ggcctggagg cagcgcgggc cgagctgcga gagatcgagg tggccctcgc cgatctcggc
     2341 ggcgacgacc gggcccgggc ccgagagctc gacctgctcc ggttccaggt ggccgagatc
     2401 gacgccgccg ccatcaccgg tcccgacgag gaccaggagc tggaggccga gggcgaccgc
     2461 ctcggcgacg ccgtggccca ccgggaggcc gcggaaggcg ccgccgacct gctgctgggc
     2521 gacgacgcgg cgcgggaccg actcgccgcc gcgggggcca cgctggccga ccgggcgccg
     2581 ttcaaggacc tggccgggcg actggtcggt ctgcaggcgg acctggagga cgtggccgcc
     2641 gcgttgcggg acgcggccga gaccattgag ccggaccccg agcgactggc tgtcgtccgg
     2701 gaacgccgcc acctgctgtc cgagctgcgc cggaagtacg gcgacacgct gggcgaggtc
     2761 ctggagttcc gggccgagac cgcggcgcgc ctgagcgagc tggagtccca cgaggaacgg
     2821 gccgccgcgt tggacgagcg ccgggcgcgg gccgagaagg tgctgggcga ggccgcagcc
     2881 aaggtcggcc gggcccgccg ggccgccgct cccgcgctgg ccgacgccgc ggtgacccgt
     2941 ctgcaggagc tggccatgcc caacgcccgc ctggcgatcg acgtcggcgc cgaccccggt
     3001 gacgacgtca ccatccagtt cagcgccaac ccgggatccg ccccccagcc gctggccaag
     3061 gtggcgtcgg gcggcgagct ggcccggacc atgctggccc ttcgcctggt caccaccgag
     3121 gccccgccca cgctcgtgtt cgacgaggtc gatgccggca tcggcgggac gaccgccttg
     3181 gccctggccg ccgccctggc ggagttgggc gacgagcacc aggtcctcgt cgtcacgcac
     3241 ctcccgcagg tggctgcctt cgccgacgcc caggtgacga tcgagaagca ggtcaccggc
     3301 gagcgcacgg ccacccaggc ccggcccgtc gacggcgacg cgcgcatcgt cgagctgtcc
     3361 cgcatgctgt cgggccagcc cgactcggcc acggcccgcc agcacgccga cgagctgctg
     3421 ggcgaggccg cggcccgacg cgccatccgg tgacggccag atgacggtgc tcctcggctt
     3481 cctgggcggt tgcgccgcgg gcttcggcgg ttggctgatg ctgcgtccca ccttcgccgc
     3541 cccgatcttc gcccgggaga accacc
//
LOCUS       DASTFV010000455         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1693683, whole genome shotgun sequence.
ACCESSION   DASTFV010000455 DASTFV010000000
VERSION     DASTFV010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693683"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFP02_08390"
     CDS             <1..210
                     /locus_tag="VFP02_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HET9543085.1"
                     /translation="AVFGVPDERWGQRVCAAVVGSATPADVQARARERLPAYKCPKAV
                     FVVDDLPHTATGKLRRLDLADRFSD"
     gene            complement(218..790)
                     /locus_tag="VFP02_08395"
     CDS             complement(218..790)
                     /locus_tag="VFP02_08395"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HET9543086.1"
                     /translation="MRRVLGLLLGAVALLVACGVPEDSAPQELSAADVPFGMLTTAPT
                     TTTTPIDLAPDREAALYFMDVEGNLRPLNREVEDRSAATVIMTLLATDSSSLPPSYST
                     NIPPDTTLLDSFVEDQVLTLDLSEQFTTVVGQRFIAAVAQIVYTATDLEGIEAVSFRV
                     EGEDFPVNDENGAAVDEPVDRLDFVGLAPS"
     gene            complement(787..2187)
                     /locus_tag="VFP02_08400"
     CDS             complement(787..2187)
                     /locus_tag="VFP02_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9543087.1"
                     /translation="MARFRYQRRLGLRARVTAAFGLGALFTAALLAATTFGISREQLI
                     RQRESASSVPFFNNARNVQERLQAEGGVDALALLQDLQTTSGSHPVLFYSGDWISTDP
                     QFGEAALPASLRRAVLDGGAAQMRYSLDGEPELGFGISLQTADDDAAYFEIVSLDETA
                     STLTSLGIILFGASVLATGTGAALGWWASRRALLPLTEVGSAAQAIAGGRLDTRLEAA
                     NDPDLARLTESFNEMAQALETRIARDEQFASDVSHELRSPLMTLSASIEVLKARKDDL
                     DERAGSALDLLVADVARFEQLVNDLLEISRLEAGAAELDLDPVDPSELVLHAVGAATS
                     AAVPVQYDEEVAGTRILVDKRRMERVVANLLDNAAKYAGGATGVRVSLAEDRVRIVVE
                     DEGPGVPEEVRQKIFDRFARGTEAGRRTKDRGVGLGLALVAEHVRLHGGQVWVEDRQD
                     GRPGAAFVVELPAVIL"
     gene            complement(2202..>2362)
                     /locus_tag="VFP02_08405"
     CDS             complement(2202..>2362)
                     /locus_tag="VFP02_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9543088.1"
                     /translation="EVLLDRIWGYGYFGDGRLVDVHVRRLRMKVEPDPANPRHVVTVR
                     GLGYKLAP"
BASE COUNT          344 a          795 c          886 g          337 t
ORIGIN      
        1 gccgtgttcg gtgtgccgga cgagcgttgg ggtcagcggg tctgcgccgc cgtcgtcggc
       61 tcggccacgc cggccgatgt ccaggcccga gctcgcgagc ggttgccggc ctacaagtgc
      121 ccgaaggcgg tcttcgtcgt ggacgacctg ccccacacgg ccacgggcaa gctccgccgc
      181 ctcgacctgg ccgaccggtt ctcggactga gcgtggctca ggagggggcg aggccgacga
      241 aatcgagtcg gtcgacgggc tcgtccacgg cggcaccgtt ctcgtcgttg acggggaagt
      301 cctcgccctc gacccggaag ctgacggctt cgatgccctc gaggtcggtc gccgtgtaga
      361 cgatctgggc cacggcggcg atgaagcgtt gacccacgac ggtggtgaac tgctcgctca
      421 ggtcgagggt caggacctgg tcttcgacga agctgtcgag cagggtcgtg tccggcggga
      481 tgttggtgct gtagctcggc ggcaggctcg aggaatcggt ggccaggagc gtcatgatca
      541 ccgtcgccgc tgaacggtcc tcgacctcgc ggttcagcgg gcggaggttt ccctcgacgt
      601 ccatgaagta gagcgcggcc tcgcggtcgg gagcgaggtc gatcggggtg gtcgtggtgg
      661 tcggcgccgt ggtcagcatc ccgaagggca cgtcggcggc gctgagctcc tgcggggcgg
      721 aatcctcggg gaccccgcag gccacgagca gggcgacggc cccgaggagc aggccgagga
      781 ctcgcctcac aggatcaccg ccggcagctc cacgacgaag gcggccccgg gacggccgtc
      841 ctgtcggtcc tcgacccaga cctggccgcc gtgcaggcgc acgtgctcgg ccaccagggc
      901 cagcccgagg cccacgccgc ggtccttggt ccgccggccg gcctccgtgc cccgggcgaa
      961 ccggtcgaag atcttctggc gcacctcctc gggaacgccg gggccctcgt cctccaccac
     1021 gatgcggacc cggtcctcgg ccaggctgac ccggacgccg gttgccccac ccgcgtactt
     1081 ggcggcgttg tccagcaggt tggccacgac ccgttccatg cgccgcttgt cgacgaggat
     1141 ccgggtgccg gccacctcct cgtcatactg cacgggcacc gcggcactgg tcgcggcccc
     1201 cacggcgtgc agcaccagct cggaggggtc gaccgggtcc aggtccagct cggctgctcc
     1261 ggcctcgagg cgggagatct ccaggagatc gttgaccaac tgctcgaagc gggccacgtc
     1321 ggccaccagg aggtcgaggg cgctgccggc ccgctcgtcg aggtcgtcct tgcgggcctt
     1381 gagcacctcg atcgaggccg agagggtcat cagcggcgag cgcaactcgt ggctgacgtc
     1441 ggaggcgaac tgctcgtccc gggcgatgcg ggtctccagc gcctgggcca tctcgttgaa
     1501 ggactccgtg aggcgggcca ggtcggggtc gttggccgcc tccaggcggg tgtcgaggcg
     1561 gccgccggcg atcgcctggg ccgcagaacc gacctcggtc agcggaagca acgcccggcg
     1621 gctggcccac caaccgaggg cggcgccggt cccggtggcc agcacggagg cgccgaagag
     1681 gatgatgccc aaggacgtga gggtgctcgc cgtctcgtcc agggagacga tctcgaagta
     1741 ggcggcgtcg tcgtcggcgg tctgcaggga gatcccgaag cccagctccg gctcgccgtc
     1801 gagcgagtag cgcatctggg ccgccccgcc gtcgagcacc gcccgccgca ggctggccgg
     1861 cagggcggct tccccgaact gcgggtcggt ggagatccag tcgccggagt agaacagcac
     1921 cggatgagaa ccgctggtgg tctgcaggtc ctggagcagg gccagcgcgt cgacaccacc
     1981 ctccgcctgc agtcgctcct gcacgttgcg agcgttgttg aagaagggga ccgacgaggc
     2041 cgactcccgt tggcggatga gctgctcgcg actgatgccg aacgtggtgg cggccagcaa
     2101 ggcggcggtg aagagggcgc cgagaccgaa ggcggcggtg acacgggccc ggaggccgag
     2161 acgacgttgg taacggaacc gggccacagt tggtggggac cctagggcgc gagtttgtag
     2221 cccaggcctc gcacggtgac cacgtggcgg gggttggccg gatcgggctc gaccttcatg
     2281 cggaggcggc gcacgtggac gtcgacgagc cgaccgtcgc cgaagtagcc gtagccccag
     2341 atccggtcca gcaggacctc gc
//
LOCUS       DASTFV010000456         3148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1697173, whole genome shotgun sequence.
ACCESSION   DASTFV010000456 DASTFV010000000
VERSION     DASTFV010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697173"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VFP02_08410"
     CDS             complement(<1..676)
                     /locus_tag="VFP02_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET9543089.1"
                     /translation="MGTRDTLPPESRRWEALVAAFAGAAGRAGFGLLQSPTFEDLGVF
                     ERVGEGTDIVTKEMYDFEDKGGRRIALRPEGTAPVVRAYLEHHPTDPWKVWYAAPTFR
                     YERPQAGRYRQHHQLGVEVLGSVDADVDVEVIALAWDFLADIGLRRVKLLINSMGEPE
                     DRVRYAGALQAYFREHLEALAEEDREKVDHHPLRVLDSKRPATAAVVADAPGPTEHLS
                     EAAAAHF"
     gene            complement(703..2913)
                     /locus_tag="VFP02_08415"
     CDS             complement(703..2913)
                     /locus_tag="VFP02_08415"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET9543090.1"
                     /translation="MPSTVDRVLPWRRSELTPDEEVAAVVEAFREHHPRAASDLVVRA
                     YRRAAAAHEGQQRKSGDPYISHPIAVAKVTADLGLDDTTIAAALLHDAVEDTRVALEE
                     LEEEFGAEVAAIVDGVTKLERLEFESREAQQAATMRKMLVAMADDLRVLMVKLADRLH
                     NMRTLAAMPTDKQLRIAQETLDIYAPLAHRLGVQEVKQQLEDLAFAALHPKRFAEVDH
                     LVDARAPERDLYLTQVEGEVTDRLAEVRVKADVTGRPKHLWSIYEKMVRGRPFDDILD
                     LVGIRVIVASVKDCYAALGCIHGMWQPVPGRFKDYIALPKFNLYQSLHTTVMGPGGKA
                     VEFQIRTQEMHQRAELGVAAHWAYKEKSPADDIVWLNKILDWQTETTDPTQFMANLKG
                     DLEDNEVYVFTPKGDVIILPDGATPLDFAYAIHTEVGHACIGARIDGRLVPLDSPLKS
                     GATVEIIASKVENPGPSRDWLHIVVTPRAQNRIRQWFSRERRGDAIEQGRDEVIKAMR
                     HDGLPMGSDVLEGVAEDLNYADLDALFAAVGERHVSAQSVATRIARMLSGSDPDSEQQ
                     LSTGLATPRRPRRSASQPARVHVEGQEQMLVRLSRCCTPVPGDEIMGFITRGRGVTVH
                     RTDCTNAVALVREQTERVIDVEWDADNQGTFVASLVVEALDREWLLRDVSAALSDHRL
                     NILHSETRTGGDRMCRMQFDVELGDPTQLDSLVSSLKNIDCVFDAYRVLPGSGN"
     gene            complement(2917..>3148)
                     /locus_tag="VFP02_08420"
     CDS             complement(2917..>3148)
                     /locus_tag="VFP02_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET9543091.1"
                     /translation="EYGSDALELHVDALGAGDTCLVIDDVLATGGTAAAVGRLIERLG
                     GRLAGYGFLIELDALGGRSQLGEGRVRTLLTY"
BASE COUNT          431 a         1069 c         1152 g          496 t
ORIGIN      
        1 cgaaatgggc ggcggcggcc tcggagaggt gctcggtcgg accgggggcg tcggccacca
       61 ccgccgcggt ggcggggcgc ttcgagtcga ggacccggag cgggtggtgg tcgaccttct
      121 cgcggtcctc ttcggccaag gcctcgaggt gctcccggaa gtaggcctgg agggcgccgg
      181 cgtagcggac ccggtcctcg ggctcgccca tggagttgat cagcagcttc acccggcgca
      241 ggccgatgtc ggcgaggaag tcccaggcca gtgcgatgac ctcgacgtcc acgtcggcgt
      301 ccaccgagcc gaggacctcg acccccagct ggtggtgctg gcggtagcgc ccggcctgcg
      361 gtcgctcgta gcggaacgtc ggggcggcgt accagacctt ccagggatcg gtcgggtggt
      421 gctcgaggta ggcccgcacc accggggcgg tgccctcggg acgcagcgcg atgcgacggc
      481 cgcctttgtc ctcgaagtcg tacatctcct tggtcacgat gtcggtgccc tcgcccaccc
      541 gctcgaagac cccgaggtcc tcgaaggtcg ggctctggag gaggccgaag ccggcccgcc
      601 cggcggcgcc ggcgaacgcg gcgaccaacg cctcccagcg ccgcgactcg ggcggcagcg
      661 tgtcgcgcgt gcccacaggg gccttgaagg tcgatgccac ggctagttgc cggagccggg
      721 caggacgcgg taggcgtcga acacgcagtc gatgttcttg agcgagctga ccagcgagtc
      781 gagctgggtc gggtcaccca gctccacgtc gaactgcatg cggcacatcc ggtcgccacc
      841 ggttcgggtc tcggagtgga ggatgttcag ccggtggtcg gacagcgccg ccgacacatc
      901 ccgcagcagc cactcgcggt ccagggcctc gacgaccagt gaggccacga aggtgccctg
      961 gttgtcggcg tcccactcga cgtcgatgac ccgttcggtc tgctcccgga ccagggccac
     1021 ggcgttcgtg cagtcggtac ggtgcacggt caccccccga ccccgggtga tgaagcccat
     1081 gatctcgtcg cccggcaccg gcgtgcagca gcgggacagt cggaccagca tctgctcctg
     1141 gccctcgacg tgcacccggg cgggctggct ggccgagcgt cgcggtcgcc ggggcgtggc
     1201 cagcccggtc gagagctgct gttcgctgtc cgggtcgctg cccgacagca tccgggcgat
     1261 gcgggtggcc accgactggg ccgagacgtg acgctcgccg accgcggcga agagggcgtc
     1321 gaggtcggcg tagttgaggt cctcggccac gccttccagg acgtcggagc ccatcggcag
     1381 gccgtcgtgg cgcatcgcct tgatgacctc gtcccggccc tgttcgatgg cgtcaccccg
     1441 tcgctcgcgg gagaaccact gccggatccg gttctgggcc cggggcgtga ccacgatgtg
     1501 cagccagtcc cgggacgggc ccgggttctc gaccttggag gcgatgatct cgacggtggc
     1561 ccctgacttc aggggcgagt cgaggggcac caggcgaccg tcgatccggg cgccgatgca
     1621 ggcgtggccc acctcggtgt ggatggcata agcgaagtcg aggggggtgg ccccgtcggg
     1681 caggatgatc acgtccccct tgggcgtgaa cacgtagacc tcgttgtcct ccaggtcgcc
     1741 cttgaggttg gccatgaact gggtcggatc ggtggtctcg gtctgccagt cgaggatctt
     1801 gttcaaccag acgatgtcgt cggcggggga cttctccttg taggcccagt gggcggccac
     1861 gcccagctcg gcccgctggt gcatctcctg ggtgcggatc tggaactcga cggccttgcc
     1921 gccggggccc atcaccgtgg tgtgcagcga ctggtagagg ttgaacttcg gtagggcgat
     1981 gtagtccttg aagcgcccgg gcaccggctg ccacatgccg tggatgcagc cgagggcggc
     2041 gtagcagtcc ttcaccgagg ccacgatcac ccggatgccg accaggtcga ggatgtcgtc
     2101 gaagggacgg ccgcggacca tcttctcgta gatgctccag aggtgcttcg gccggccggt
     2161 cacgtcggcc ttgacccgga cctcggccag ccgatcggtg acctcgccct ccacttgggt
     2221 caggtagagg tcccgctcgg gggcccgggc gtcgaccagg tggtcgacct cggcaaagcg
     2281 cttcgggtgc agggcggcga aggccaggtc ctccagctgc tgcttgacct cctgcacacc
     2341 gaggcggtgg gccagcgggg cgtagatgtc cagggtctcc tgggcgatgc gcagctgctt
     2401 gtccgtgggc atcgcggcca acgtgcgcat gttgtgcagg cggtcggcca gcttgaccat
     2461 gaggacccgg aggtcgtcgg ccatggccac cagcatcttg cgcatggttg cggcctgctg
     2521 ggcctcccgg gactcgaact ccagccgctc gagcttggtg acgccgtcga cgatggccgc
     2581 cacttcggcg ccgaactcct cctccagctc ctcgagggcg acccgggtgt cctccaccgc
     2641 gtcgtgcagg agggcggccg cgatggtggt gtcgtccagg ccgaggtcgg ccgtgacctt
     2701 ggccacggcg atcgggtggg agatgtacgg atcgcccgac ttgcgctgtt ggccctcgtg
     2761 ggccgcggcc gcccgtcggt aggcccgcac gaccaggtcg ctggccgccc gcgggtggtg
     2821 ctcgcggaag gcctcgacga cggcggcgac ctcctcgtcc ggggtcagct cactgcgccg
     2881 ccagggcagg acgcggtcga cggtggaggg catcggtcag tacgtcagga gggtccgcac
     2941 acggccctcc cccagctggc tgcgcccgcc gagcgcatcg agttcgatca agaagccata
     3001 gccggcgaga cggccgccca ggcgctcgat cagtcgcccg accgccgccg cggtgccgcc
     3061 ggtggccaac acgtcgtcga tgaccaggca ggtgtccccc gcccccaggg cgtcgacgtg
     3121 cagctccagg gcatcgctgc cgtactcc
//
LOCUS       DASTFV010000457         2586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1698412, whole genome shotgun sequence.
ACCESSION   DASTFV010000457 DASTFV010000000
VERSION     DASTFV010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698412"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VFP02_08425"
     CDS             complement(<1..672)
                     /locus_tag="VFP02_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543092.1"
                     /translation="MLSKLDDYPVHQTVLPLAIPNTSDRHSYDRYWFNGYDRDGEFYF
                     GIALCRYPNLGILDCAFSIIRGGEQHSFHGSRRMPLEPTDLTVGPFELQILEPMGRTR
                     LVLDDNETGIAADLTLTPRTAAVEEGRQTLIRDDRVVLDSTRFAQFGHWEGEISYAGE
                     TLKVNGATTPGTKDRSWGVRPVGDPAPPGAPSLDFTGIFFLWAPIHWEDRCTHFLVFE
                     EPDGRP"
     gene            complement(725..1051)
                     /locus_tag="VFP02_08430"
     CDS             complement(725..1051)
                     /locus_tag="VFP02_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9543093.1"
                     /translation="MPTDVTKAVLDHHTEMLDAGDVDGLMEDYAEDAVFISNLGGVVS
                     GLDAIRGIFAATAGGVAGFEAGVEHVDGEVGYVTWKAEGIPFGTDTFVVRGGKIAVQT
                     VALHFG"
     gene            complement(1084..>2586)
                     /locus_tag="VFP02_08435"
     CDS             complement(1084..>2586)
                     /locus_tag="VFP02_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HET9543094.1"
                     /translation="APSARSRSTNGGSGMNLMMLLDMATQVAGERVAFGTAEGGLTYQ
                     QLFDRAGGAARRLQEDEVGTLAFVGTATDAFPTAVFAAAWAGVPLAPLNYRLSDEQLT
                     ALLARLDRPKIVADPQFVDRLRALGAEVEPTVDWVAAALATEVADPWAMDPTAVALLL
                     FTSGTTAEPKAAVLRQEHLCSYVLGSVEFASADETAAALVSVPPYHIAGVSNVVSNVY
                     SNRRVVLLPQFDPAAWLATARAEAVTHALVVPTMLARVVEYLDGAPADLPALRSLAYG
                     GAPMPVTVLERALAAFGDTDFVNAYGLTETSSTIAVLGPDDHRTALASDDPAVRARLG
                     SAGRLLPTIEADVRDGLLWVRGEQVSGEYAGMDSVLDDEGWFPTGDVAHIDGEGFLFI
                     EGRADDTIIRGGENIAPAEIETVLLRHDSVAECAVVGLPDEEWGQRIAAVVVTTPDAD
                     PDEAELQAWCREHLRGSKTPDRIEFRAELPYTDTGKLLRRTLRTELVDGD"
BASE COUNT          368 a          881 c          968 g          369 t
ORIGIN      
        1 cgggcggccg tcgggctcct cgaagacgag gaagtgcgtg caccggtcct cccagtggat
       61 gggggcccac aggaagaaga tcccggtgaa gtccagcgac ggcgcgcccg gcggggcggg
      121 atcgccgacc ggacggacgc cccacgagcg gtccttggtg cccggcgtcg tggccccgtt
      181 gaccttcagc gtttcgccgg cgtaggagat ctcgccctcc cagtggccga actgggcgaa
      241 gcgcgtggag tcgaggacga cccggtcgtc ccggatcagg gtctgacgcc cctcctccac
      301 cgccgcggtc cggggggtga gcgtgaggtc ggccgcgatc ccggtctcgt tgtcgtcgag
      361 aacgagccgg gtccggccca ttggctcgag gatctggagc tcgaaggggc cgacggtcag
      421 gtcggtgggc tcgaggggca tgcgccggga accgtggaac gagtgctgct cgccgccccg
      481 gatgatggag aaggcgcagt ccaggatccc caggttcggg taccgacaca gggcgatgcc
      541 gaagtagaac tcgccgtccc ggtcatagcc gttgaaccag taccggtcgt agctgtgccg
      601 gtcggaggtg ttcgggatgg ccagaggcag gaccgtctgg tgcaccgggt agtcgtcgag
      661 cttcgagagc atgctccatc ttgaccgagc cggcggcgtg cggctgacgg acggctcgac
      721 cgggtcagcc gaagtgcagg gccaccgtct gaacggcgat cttgccgcca cggaccacga
      781 aggtgtcggt gccgaacggg atgccttcgg ccttccaggt cacgtagccg acctcaccgt
      841 cgacgtgctc gacccccgct tcgaagccgg cgacgccccc cgcggtggcg gcgaagatgc
      901 cgcgaatggc gtcgaggccc gagaccacgc caccgaggtt gctgatgaag accgcgtcct
      961 cggcgtagtc ctccatcagg ccgtcgacgt caccggcgtc gagcatctcc gtgtggtggt
     1021 cgaggaccgc cttggtcaca tccgtgggca tcgctggtct cctgtcggtt cgggccccgt
     1081 gttctagtcg ccgtcgacca gctcggtgcg cagcgtgcgg cggaggagct tgccggtgtc
     1141 ggtgtagggc agctccgctc ggaactcgat gcggtcgggg gtcttggagc cacggaggtg
     1201 ctcccggcac caggcctgca gctcggcctc gtcagggtcg gcgtctggcg tggtcaccac
     1261 gacggcggct atgcgttgcc cccactcctc gtcggggagg ccgaccaccg cacactcggc
     1321 gacggagtcg tggcggagga ggaccgtctc gatctccgcc ggggcgatgt tctccccgcc
     1381 ccggatgatg gtgtcgtcgg cccggccctc gatgaagagg aagccctcac cgtcgatgtg
     1441 ggcgacgtcg cccgtcggga accagccctc gtcgtccagc acggagtcca ttccggcgta
     1501 ttcccccgac acctgctcgc cgcgcaccca gaggaggccg tcgcggacgt cggcctcgat
     1561 ggtgggcagc agccggccgg cggagccgag gcgggcccgg acggcggggt cgtcggacgc
     1621 cagggccgtg cggtggtcgt cggggccgag cacggcgatg gtcgacgagg tctcggtcag
     1681 gccgtaggcg ttgacgaagt cggtgtcgcc gaacgccgcc agtgcccgct ccagcacggt
     1741 cacgggcatc ggggccccgc cgtaggccag ggagcgcagc gcgggcaggt cggcgggcgc
     1801 cccgtcgagg tactcgacga cccgggccag catggtgggc acgaccaggg cgtgggtcac
     1861 ggcctcggcc ctcgcggtgg ccagccaggc cgcggggtcg aactgcggga gcaggaccac
     1921 gcgacggttc gagtagacgt tcgagaccac gttcgacacg ccggcgatgt ggtacggcgg
     1981 cacgctgacc agcgccgccg ccgtctcgtc ggccgacgcg aactccaccg agccgaggac
     2041 gtaggaacag aggtgctcct ggcggaggac ggcggccttg ggctcggccg tggtcccgct
     2101 ggtgaagagc aggagggcca cggccgtcgg atccatggcc caggggtcgg cgacttcggt
     2161 ggcgagggcc gcggcgaccc agtcgacggt cggctccacc tcggcgccga gcgcccggag
     2221 ccggtcgacg aactgggggt cggccacgat cttcggccgg tccagccggg cgaggagggc
     2281 cgtcagctgc tcgtcgctga ggcgatagtt caggggcgcc aacgggacgc ccgcccaggc
     2341 cgcggcgaac accgccgtcg ggaaggcatc ggtggccgtg ccgacgaagg ccagcgtgcc
     2401 cacctcgtcc tcctggaggc gccgagccgc tccgccggcc cggtcgaaga gctgctggta
     2461 ggtcagtccg ccctcagccg tgccgaacgc cacccgctcc cccgccacct gggtggccat
     2521 gtccaacaac atcatcaggt tcatgcccga ccccccgttc gtcgaccggc tccgggcgct
     2581 cggcgc
//
LOCUS       DASTFV010000458         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1709849, whole genome shotgun sequence.
ACCESSION   DASTFV010000458 DASTFV010000000
VERSION     DASTFV010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709849"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFP02_08440"
     CDS             complement(<1..479)
                     /locus_tag="VFP02_08440"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9543095.1"
                     /translation="MTEAFTLDPFQQQAVAAIDRGESVLVAAPTGAGKTVVAEHAVER
                     VLAEPDGRVFYTTPVKALSNQKYGDLVRRHGPDRIGLLTGDNSINGDARVVVMTTEVL
                     RNMIYAGRPLDDLRFVVLDEVHYLQDAYRGPVWEEVIIHVPVHVRLVCLSATVSNAD"
     gene            complement(476..1234)
                     /gene="tatC"
                     /locus_tag="VFP02_08445"
     CDS             complement(476..1234)
                     /gene="tatC"
                     /locus_tag="VFP02_08445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET9543096.1"
                     /translation="MSATDEAQELTGGHMSLIEHLTELRSRLIKCVIAVAVGAVIVWI
                     FYDPILTFLKAPLDEADPNARLILTDPLQGLATRFKVSAYGGIALATPVLLWQLWKFI
                     TPGLYPKERRYAVPFVASGVVLFIMGASLAYWTLPRALAFLISIGGPDIEAFYTPDKY
                     IQLIVYMMLAFGLGFEFPIVLVFLQLVGVLSYQRLISWRRYAIIGIVVLVAVITPSGD
                     PISLAALSVPMYLFYELSILIGWLITRRRVTAET"
     gene            complement(1246..1533)
                     /locus_tag="VFP02_08450"
     CDS             complement(1246..1533)
                     /locus_tag="VFP02_08450"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET9543097.1"
                     /translation="MFNVGGPEVLVILLVALIALGPAQLPKAARQVGQVMAEIRKVSS
                     GFQRELTDAFDLESKSEKPEKPQSPGGPQDVAPPSDGLPDAGPAPGDDDPA"
     gene            complement(1544..1747)
                     /locus_tag="VFP02_08455"
     CDS             complement(1544..1747)
                     /locus_tag="VFP02_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543098.1"
                     /translation="MVWLWLLFPLVVLLTAVALWRANGRLRAEQRSLQLEVDALAPLG
                     EAARSLPVDGECGRAAGQKQADR"
     gene            complement(1776..2114)
                     /locus_tag="VFP02_08460"
     CDS             complement(1776..2114)
                     /locus_tag="VFP02_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543099.1"
                     /translation="MATIRASCPDCGDVEFTTHDVQVRVSAPDGSGTYAFSCPGCAVT
                     VVKAAETRTIDLLLASGVVMAAGDRPCEIDERPYFEDPITHDDLLDFHHMLHRDDSWF
                     ESLAQDNDAS"
     gene            2209..>2438
                     /locus_tag="VFP02_08465"
     CDS             2209..>2438
                     /locus_tag="VFP02_08465"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HET9543100.1"
                     /translation="MARHPRLLDIPIGFAHRGASAAAPENTLEAFRLAVELGASGLES
                     DVWVSNDGEAVLDHDGLVGGWLRRRPMGTVLR"
BASE COUNT          405 a          787 c          889 g          357 t
ORIGIN      
        1 tcgtcggcgt tggacaccgt ggccgacagg cacacgagcc ggacgtggac cggcacgtgg
       61 atgatgacct cctcccagac cgggccccgg taggcgtcct ggaggtagtg gacctcgtcg
      121 agcacgacga agcgcaggtc gtccagcggc cgcccggcgt agatcatgtt ccggagcacc
      181 tcggtggtca tcaccacgac ccgggcatcg ccgttgatgc tgttgtcgcc ggtcagcagc
      241 ccgatccgat cggggccatg gcgacggacc aggtcgccgt acttctggtt cgacagggcc
      301 ttcaccggcg tcgtgtagaa gacacgcccg tcgggctcgg ccaggacccg ctccactgcg
      361 tgctcggcca cgaccgtctt gccggcgccc gtcggggcgg cgaccagaac cgactcgccc
      421 cggtcgatgg cggcgacggc ctgttgctgg aacgggtcga gcgtgaaggc ctcggtcacg
      481 tctcggcggt cacgcggcga cgggtgatga gccagccgat caggatcgag agctcataga
      541 agaggtacat cggcaccgag agggcggcca ggctgatcgg atcaccgctg ggcgtgatca
      601 cggcgaccag cacgacgatg ccgatgatgg cgtaccggcg ccaggagatc aggcgctggt
      661 agctgagcac gccgaccaac tggaggaaga ccaggacgat cgggaactcg aagcccaagc
      721 cgaacgccag catcatgtag acgatgagct ggatgtactt gtccggcgtg tagaacgcct
      781 cgatgtccgg accgccgatc gagatgagga aggcgagcgc ccggggcagg gtccagtacg
      841 ccaggctggc gcccatgatg aacaggacca cgcccgaggc cacgaacggc acggcgtagc
      901 gccgctcctt cgggtacagc ccgggggtga tgaacttcca cagctgccag agcaggaccg
      961 gcgtggccag ggcgatgccg ccgtacgcgg acaccttgaa gcgggtggcc agcccctgga
     1021 gggggtcggt gaggatgagg cgggcgttgg ggtcggcctc gtcgagcggc gccttcagga
     1081 aggtgaggat ggggtcgtag aagatccaca cgatcaccgc gcccacggcc acggcgatca
     1141 cgcacttgat gaggcggctg cgtagctcgg tgaggtgctc gatgagggac atgtgtcccc
     1201 cggtgagctc ctgggcttcg tctgtggcgc tcacgcggtc ggcgctcagg ccggatcgtc
     1261 gtcgccggga gcggggccgg cgtccgggag accatcgctc ggcggggcga cgtcctgggg
     1321 accgccgggc gactgcggct tctcgggctt ctccgacttc gactccaggt cgaacgcgtc
     1381 agtgagctcg cgctggaagc ccgaggagac cttgcggatc tcggccatga cctggccgac
     1441 ctggcgggcg gccttgggca gctgggccgg gcccagggcg atcaacgcca ccaagaggat
     1501 gacgagcacc tcgggcccgc cgacgttgaa catcggacga atcctaccgg tcggcctgct
     1561 tctgcccggc ggcacgtccg cactcgccgt cgaccgggag cgaccgggcc gcctccccca
     1621 gcggggccag ggcgtcgacc tcgagctgaa gggagcgctg ctcggcgcgg agccgcccgt
     1681 tggcccgcca cagggcgacg gcggtgagaa gcaccacgag ggggaacagc aaccagagcc
     1741 agaccatcaa cggtcagtct ggccgaacga cctcgtcagg aggcgtcgtt gtcctgggcc
     1801 agcgactcga accagctgtc gtcgcggtgg agcatgtggt ggaagtcgag gaggtcgtcg
     1861 tgggtgatcg ggtcctcgaa gtagggacgc tcgtcgatct cacacgggcg gtcacctgct
     1921 gccatgacga cgccgctggc cagcaggaga tcgatggtgc gggtctccgc cgccttgacg
     1981 accgtcacgg cgcagccggg acaggagaag gcgtaggtgc cggagccgtc cggcgcactg
     2041 acccggacct gcacgtcgtg ggtcgtgaac tccacgtcac cgcagtccgg gcaacttgcc
     2101 ctgatcgtcg ccatgtcgtc tcgtccccag tccttgtact tcctccagca agggagccat
     2161 cggcacggcg ttggaggccc ttgagcaacc gcggcgagaa tgggccatat ggcccgtcac
     2221 ccccgcctgc ttgacattcc gatcggtttc gcccaccggg gtgcgagcgc ggcggctccc
     2281 gagaacaccc tcgaggcctt tcgcctggcg gtggaactgg gggcatccgg gctggaatcc
     2341 gacgtctggg tcagcaacga cggggaggcc gtgctcgacc acgacggcct ggtcgggggc
     2401 tggttgcggc ggcgacccat ggggacggtg ctgcgcca
//
LOCUS       DASTFV010000459         2761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1711403, whole genome shotgun sequence.
ACCESSION   DASTFV010000459 DASTFV010000000
VERSION     DASTFV010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711403"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1480)
                     /locus_tag="VFP02_08470"
     CDS             complement(74..1480)
                     /locus_tag="VFP02_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9543101.1"
                     /translation="MTDVSMTIGGERVDAPSTFGVVNPATGEIHAEAPDCGKEQLDQA
                     FDAAAKAFVDWRRDEGARREALKAASAALGAAGERLGPILTAEQGKPIAAAQMEAMAG
                     GYWLRYFAKLEMPREVIQDDDAAYAEVVRRPMGVVAAITPWNFPIVLASWKIGPALLA
                     GNTMVLKPSPFTPRSTLLMGEILNEVLPPGVLNVVSGGDELGQWMTAHPTPRKVSFTG
                     SVATGKHVNASAAPDLKRVTLELGGNDPAILLDDVDPGDIAEKLYAGAFDNCGQVCSA
                     IKRVYVPEALHDDVVDALSARVAAAKVGDGMDPESDLGPVQNLPQYERVSELVAEALD
                     GGASAATGGAPIDGPGYFFQPTVLTGAAEGTRIVDEEQFGPALPVIPYRDIDDVVDRA
                     NATTYGLSGSVWSPDVERAGAVAERLECGTAWVNAHVALGPHQPFGGFKWSGLGVENG
                     PWGLAGFTELQVQYRAKG"
     gene            complement(1595..2428)
                     /locus_tag="VFP02_08475"
     CDS             complement(1595..2428)
                     /locus_tag="VFP02_08475"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET9543102.1"
                     /translation="MSVTTQQITPEIGLEVTGLAGHELVDPARAAECQAALDAHGVVV
                     YREAGIGDADLLAFTEMLGTVVIPKMGGLEEHPGVDVISLDPAESSLAKYRRSTIFWH
                     LDGTTDELPQKATLLTALVVADEGGDTEFAHTYAAYAALPEDEKARLADLRVEHSLAA
                     AQLMFTPEPTEKQRAAWDQVPSRVHPLVWRRRDGRRSLLIGSTAGRIVDLPEDESRAL
                     LDHLLEWVTQPRFVLRHHWRPGDLVVWDNTGLLHRAIPYEATSHRLLHRTTLVGEEAI
                     A"
     gene            2532..>2761
                     /locus_tag="VFP02_08480"
     CDS             2532..>2761
                     /locus_tag="VFP02_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9543103.1"
                     /translation="MTASIDELVAEYSGEDLGLAHVLCDRHPADAPAFTVVEADRHAV
                     TLTYGQLRESSERLAAAFAELGLGPGDRIATL"
BASE COUNT          382 a          945 c         1015 g          419 t
ORIGIN      
        1 acccggggta cccgggtccc cacgtagcgg gccgcgacgg tcgccggcgc tgggtccatg
       61 gctgcggttg ccgtcagccc ttggcgcggt actggacctg gagctcggtg aagccggcca
      121 ggccccaggg gccgttctcg acgccgagcc cgctccactt gaagccaccg aagggctggt
      181 gggggccgag ggcgacgtgg gcgttgaccc aggccgtgcc gcactcgagg cgctcggcca
      241 cagcaccggc ccgctcgacg tcgggagacc acaccgagcc cgacaggccg tacgtggtgg
      301 cgttggcgcg gtccacgacg tcgtcgatgt cgcggtacgg gatcaccgga agggccggac
      361 cgaactgctc ctcgtccacg atccgggtcc cctcggccgc gccggtgagc acggtgggct
      421 ggaagaagta gccgggaccg tcgatcggcg caccgccggt cgcggccgat gcgcccccgt
      481 cgagggcttc ggcgaccagc tcgctgaccc gctcgtactg cgggaggttc tggaccggac
      541 cgaggtcgct ctccggatcc atgccgtcgc cgaccttggc cgccgccacc cgggccgaga
      601 gggcatcgac cacgtcatcg tgcagggcct cgggcacgta cacccgcttg atggccgagc
      661 agacctgccc gcagttgtcg aaggcgccgg cgtaaagctt ctcggcgatg tcgcccgggt
      721 cgacatcgtc gagcaggatc gcggggtcgt tgccgcccag ctcgagggtg acccgcttga
      781 ggtcgggggc ggccgaggcg ttcacgtgct tgccggtggc caccgacccc gtgaagctca
      841 ccttgcgggg cgtcgggtgg gcggtcatcc actggcccag ctcgtcgccg ccggacacca
      901 cgttgagcac gcccggcggg aggacctcgt tgaggatctc gcccatgagc agcgtgctgc
      961 ggggcgtgaa gggcgagggc ttcaggacca tcgtgttgcc ggccagcagc gcgggcccga
     1021 tcttccagga cgcgagcacg atcgggaagt tccacggcgt gatggccgcg accacgccca
     1081 tcgggcgacg gacgacctcg gcgtacgcgg cgtcgtcgtc ctggatcacc tcgcggggca
     1141 tctccagctt ggcgaagtag cgcagccagt agcccccggc cattgcctcc atctgcgccg
     1201 cggcgatcgg cttgccctgc tctgcggtca ggatcgggcc gagccgttca ccggcggcgc
     1261 cgagcgcggc ggacgccgcc ttcagggctt cgcggcgggc tccctcgtcc cggcgccagt
     1321 cgacgaacgc cttggccgcg gcgtcgaacg cctggtccag ctgctccttg ccgcagtccg
     1381 gggcttcggc gtggatctca cccgtggcgg ggttgacgac gccgaaggtc gatggcgcgt
     1441 ccacgcgctc gccgccaatg gtcatcgaga cgtcggtcat gtctgctcct cgttcggatg
     1501 tcggcgagtc gaccgtagac ggtcgactgt gctgaacatc tgtttagcat acgctcgggg
     1561 cgagctcgtt gcccgtgggc tcggggacaa gagatcaggc gatcgcttcc tcgcccacga
     1621 gggtggtgcg gtgcagcagc cggtgggagg tggcctcgta ggggatggcc cggtggagca
     1681 ggccggtgtt gtcccagacg acgagatcgc ccgggcgcca gtggtgacgg aggacgaagc
     1741 ggggctgggt cacccactcg agcaggtggt cgagcagggc ccggctctcg tcctccggta
     1801 ggtccacgat ccggcccgcc gtggatccga tcaggagcga ccggcggccg tcccggcggc
     1861 gccaaaccag ggggtgcacc cggctcggca cctggtccca ggccgcccgc tgcttctccg
     1921 tcggctcggg cgtgaacatg agctgggcgg cggcgaggct gtgctcgacc cggaggtcgg
     1981 cgagccgggc cttctcgtcc tcggggaggg cggcgtaagc ggcgtacgtg tgggcgaact
     2041 cggtgtcacc gccctcgtcg gcgaccacca gggcggtgag cagcgtcgcc ttctgcggga
     2101 gctcgtcggt cgtgccgtcg aggtgccaga agatcgtgct ccggcggtac ttggcgaggg
     2161 agctctcggc cggatcgagg ctgatgacgt cgaccccggg gtgctcctcc agcccaccca
     2221 tcttcgggat gacgacggtg ccgagcatct cggtgaaggc caggaggtcg gcgtcgccga
     2281 tgccagcctc ccggtagacg accaccccat gggcgtccag ggcggcttgg cactccgccg
     2341 cccgggcggg gtcgaccagc tcatgaccgg ccaggccggt cacctcgagg ccgatctcgg
     2401 gcgtgatctg ctgggtcgtg acgctcatga gaacgagatt atcactccag cagtacgccg
     2461 ttctagtggc actattggac ggcaggcagc ccgagcgcta aacacctgtt tagcggcgca
     2521 ggaggcacga catgaccgcg agcatcgacg agctggtggc cgagtacagc ggggaggacc
     2581 tgggcctcgc ccacgtgctc tgcgaccgac atccggcgga cgcgccggcg ttcaccgttg
     2641 tggaggccga ccgccacgcc gtgacgctga cctacggaca gctccgcgaa tcctccgagc
     2701 ggctggcggc ggccttcgcc gagctcgggc tcggccccgg cgaccggatc gccaccctga
     2761 t
//
LOCUS       DASTFV010000460         3930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1714023, whole genome shotgun sequence.
ACCESSION   DASTFV010000460 DASTFV010000000
VERSION     DASTFV010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714023"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFP02_08485"
     CDS             complement(<1..531)
                     /locus_tag="VFP02_08485"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543104.1"
                     /translation="MTTFPSGLLPMKAVNGTLLPEDDGGWAFEIKWDGMRLLAYVDPG
                     AATPLRLQTTRGHDAVPRYPELAGLADAVGRPAVLDGEVVVLDENGRSDFGRLQRHED
                     AVTFIVFDLLWLDGNDVTALPYLDRRRLLADVVEDGEGWLVPTHLVGDGAVLFEQARA
                     NGLEGLVAKRVDSPYLS"
     gene            complement(528..1409)
                     /locus_tag="VFP02_08490"
     CDS             complement(528..1409)
                     /locus_tag="VFP02_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9543105.1"
                     /translation="MGDRVLAIDIGGTKLACGLVDEHGTVAARATRPTGDETHGDALF
                     ATLAGLIDEVVARAQPTDALVACGVGSGGPMTGGGASVSPLNIVAWRDFPLLDRLRAH
                     TGLATTIDGDAKALALGEGWLGAAAGARDYLAMVVSTGVGGGIVLDGRLLDGADGNAG
                     HIGHVMVEPDGALCGCGARGCLEAEASGTAIARATGRPPAEADLTVRRRTGRLVGRAV
                     ASVANLLDLPLVVVAGSVALGYGEDFFGAAQAEIDDRCRLEFSRNTVIRPGGLGADGP
                     LLGAAAVGWRALGRIPG"
     gene            complement(1436..2308)
                     /locus_tag="VFP02_08495"
     CDS             complement(1436..2308)
                     /locus_tag="VFP02_08495"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HET9543106.1"
                     /translation="MPGADESIELSVGGQALIEGVMMRAGTRWGAAARRPDGAIVTTG
                     DAVPTWARRTDNVPIVRGAVAMVETIMVGLRALKWSAQVADPDEDAEITNRSLAITTA
                     TALLIFATIFAVVPAAVSRALGFEGAVFTTVEAALRLALFGGYLLLIGRSPGIARTFA
                     YHGAEHMVISAHEHGADLTVEGVRPFSVRHARCGTDFLLLVVVVALVVFSFVGDASWP
                     VLIASRLLGLPLVAGLAYEVLKLAKRRPDSKVVAVLTAPGLALQAMTTRPPDDEQIEV
                     AIAALAAVQTGSDR"
     gene            2366..2815
                     /locus_tag="VFP02_08500"
     CDS             2366..2815
                     /locus_tag="VFP02_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HET9543107.1"
                     /translation="MNRRSRSRVVAVLLAGTLVLTLVGGLLSATGNQPRTGDDATTPS
                     TDSAPPVTQASDLPPIAYSELPTEGQNTLQLIGAGGPYPYDRDGVVFENREGLLPEEP
                     QGYYKEYTVPTPGESGRGARRIIVGGDRVVAYYTDDHYESFREVVSG"
     gene            2808..3212
                     /locus_tag="VFP02_08505"
     CDS             2808..3212
                     /locus_tag="VFP02_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HET9543108.1"
                     /translation="MADLHEVFSGAVEPGLHPLEQTVTAEELAEMAAGHGWTPIVVDL
                     AGVADKAGLLDRVAEGARFPEYAGRNWDALQDALGDLAWLGPTDGYLVVLDGWAGFAG
                     AAPGDAAVLESVVTRAGAEWSSRGTPLVAVTL"
     gene            3237..3311
                     /locus_tag="VFP02_08510"
     tRNA            3237..3311
                     /locus_tag="VFP02_08510"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3272..3274,aa:Lys,seq:ttt)
     gene            complement(3319..>3930)
                     /locus_tag="VFP02_08515"
     CDS             complement(3319..>3930)
                     /locus_tag="VFP02_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9543109.1"
                     /translation="EDIAGATEGDDPVTIARESVLRIRAVGACGVAIGSGFVVDGRHL
                     VTNRHVVAGARRLQVETWDGRRVAVGDARQAVDTDLGVIDLPARSARRLEPLALADEP
                     TPVGARLTALGYAMAGPAVSTQGQFLDRPRGRRFEEPARVMRMSASVRPGNSGGPLLN
                     ADGEVVGVVFAYETATLNSLAIPRDRLEDVLADPDALEPVVPC"
BASE COUNT          550 a         1388 c         1502 g          490 t
ORIGIN      
        1 cgacaggtag gggctgtcga cccgcttggc gaccagcccc tcgagcccgt tggccctggc
       61 ctgctcgaag aggacggcgc cgtcccccac gaggtgggtg gggaccagcc aaccctcgcc
      121 gtcctcgacc acgtcggcca gcaggcgcct tcggtcgagg taggggaggg ccgtgacatc
      181 gttgccgtcg agccacagga ggtcgaagac gatgaacgtg acggcgtcct cgtgccgctg
      241 caggcgaccg aagtcggagc ggccgttctc atcgagcacg accacctcgc cgtcgagcac
      301 ggcgggacgg cccacggcgt cggccagacc ggcgagctcc gggtaccggg ggacggcgtc
      361 gtggcccctg gtcgtctgca ggcgcagggg agtggcggca ccggggtcga cgtaggccag
      421 caggcgcatg ccgtcccact tgatctcgaa ggcccatccc ccgtcgtcct cgggcagcag
      481 ggtgccgttg accgccttca tgggcaggag gcccgacggg aaggtcgtca tcccgggatg
      541 cggccgaggg cccgccagcc cacggcggcg gcgccgagca ggggaccgtc ggcgcccaac
      601 ccacccgggc ggatgacggt gttccggctg aactcgagcc ggcaccggtc gtcgatctcg
      661 gcctgcgccg cgccgaagaa gtcctcgccg tagcccaggg cgaccgagcc ggccacgacc
      721 acgagtggca ggtcgaggag attggccacc gaggccacgg cccggcccac cagccggccg
      781 gtgcgccggc gcacggtgag atcggcttcg gcgggggggc ggccggtcgc ccgggcgatg
      841 gccgtgccgg acgcctccgc ctcgaggcag ccccgggccc cgcagccgca cagggcgccg
      901 tcgggctcga ccatgacgtg gccgatgtgg ccggcgttgc cgtcggctcc gtcgaggagg
      961 cggccgtcca gcacgatccc gccccctacg ccggtcgaca ccaccatggc caggtagtcc
     1021 cgggctccgg cggcggcacc cagccagccc tccccgaggg cgagggcctt cgcgtcgccg
     1081 tcgatggtgg tggccaggcc ggtatgggcc cgcagccggt cgaggagcgg aaagtcccgc
     1141 caggccacga tgttcagcgg ggagacgctg gcgccaccac cggtcatcgg cccgccggag
     1201 ccgacgccac aagcgacgag ggcgtcggtc ggctgggcgc gggccaccac ctcgtcgatc
     1261 aggccggcca gggtggcgaa cagtgcgtca ccgtgggtct cgtccccggt gggtcgggtg
     1321 gcgcgggccg cgacggtccc gtgctcgtcg accagcccgc aggccagctt cgttccgccg
     1381 atgtcgatgg ccagcacccg gtcgcccacg gctcaagtgt gggcgatttc ggccgctagc
     1441 ggtccgatcc ggtctgcacc gccgccagag cggcgatggc gacctcgatc tgctcgtcgt
     1501 ccgggggccg ggtggtcatg gcctgcaggg ccagccccgg cgcggtgagg acggcgacga
     1561 ccttgctgtc gggccggcgc ttggccagct tcagcacctc gtaggccagg ccggcgacca
     1621 agggcaggcc gagcagacgg gacgcgatca ggaccggcca cgaggcgtcc ccgacgaagc
     1681 tgaacaccac gagggccacg acgaccacga ggagcaggaa gtcggtcccg cagcgggcgt
     1741 gacggacgct gaacgggcgg acgccctcga cggtcaggtc ggcgccgtgc tcgtgggccg
     1801 agatgaccat gtgctctgcg ccgtgatagg cgaaggtgcg ggcgatgccg ggcgagcggc
     1861 cgatgagcag gaggtagcca ccgaacaagg cgagccgcag cgcggcctcg accgtggtga
     1921 agacggcgcc ctcgaagccc agggcccggg acacggccgc ggggaccacg gcgaagatgg
     1981 tggcgaagat cagcagggcg gtggccgtgg tgatggccag cgagcggttg gtgatctcgg
     2041 cgtcctcgtc cgggtcggcc acctgcgccg accacttcag ggcccggagc ccgaccatga
     2101 tcgtctcgac catggccacg gccccccgga cgatcggcac gttgtcggtg cgccgggccc
     2161 aggtgggcac cgcgtcgccc gtggtgacga tcgcaccgtc ggggcgacga gccgcggccc
     2221 cccagcgggt gccggcccgc atcatcaccc cttcgatcag ggcctgaccg cccacgctga
     2281 gctcgatcga ctcgtccgct ccgggcatca tccccccgtc ggcggggagg gggccggcct
     2341 tgaggggccc ggtaggttcc cgcccgtgaa ccgtcgctcc cggtcccgcg tcgtggcggt
     2401 ccttctggcg gggacgctgg tcctgaccct ggtgggcggc ctgctgtcgg ccacgggcaa
     2461 ccagccacgc acgggcgacg acgcgacgac cccgagcacc gactccgccc ccccggtcac
     2521 ccaggccagc gacctgccgc cgatcgcgta cagcgagctg ccgacagagg gacagaacac
     2581 cctgcagctg atcggggcag gggggcccta cccgtatgac cgggacgggg tggtcttcga
     2641 gaaccgcgaa ggcctgctcc cggaagagcc ccagggctat tacaaggagt acaccgtgcc
     2701 cacgcctggt gagagcggcc gaggtgctcg tcgcatcatc gtggggggtg accgggtggt
     2761 ggcctactac accgacgacc actacgagag cttccgcgag gtcgtcagtg gctgacctgc
     2821 acgaggtgtt cagcggtgcg gtggagccgg gcctgcatcc cctcgagcag accgtgacgg
     2881 ccgaggagct ggccgagatg gccgccggcc acggctggac cccgatcgtg gtcgaccttg
     2941 ccggcgtcgc cgataaggcc ggcctgctgg accgggtggc cgagggcgcc cggttccccg
     3001 agtacgccgg ccgcaactgg gacgccctgc aggacgccct gggcgatctc gcctggctgg
     3061 gcccgaccga cggctatctc gtggtgctcg acggctgggc cgggttcgcg ggcgcggccc
     3121 cgggggacgc ggccgtgctg gagtcggtgg tgacgcgggc gggagcggaa tggtcgtcgc
     3181 ggggcacgcc gttggtggcc gtcacgctct aggggtggcg gacctacgct cggagggccc
     3241 ccgtagccca gcctggtcag ggcaacggac ttttaatccg gagatcgtgg gttcgaatcc
     3301 caccgggggc acgcaggctc agcaggggac cacgggctcg agggcgtcgg ggtcggccag
     3361 gacgtcctcc aggcggtcgc gagggatggc gagggagttc agggtggccg tctcgtaggc
     3421 gaagacgacg ccgacgacct cgccatcggc gttcagcagg ggcccaccgg agttgccggg
     3481 acggacgctc gcgctcatcc gcatgacccg ggcgggctct tcgaagcggc gcccgcgggg
     3541 tcggtcgagg aactggccct gggtgctgac cgccgggccc gccatggcgt agcccagggc
     3601 cgtgagccgg gccccgaccg gtgtcggttc gtcggccaag gccagcggct cgagccggcg
     3661 ggcgctgcgg gcgggcaggt cgatgacgcc gaggtcggtg tccacggcct gacgggcgtc
     3721 gcccacggcc acccgccgtc cgtcccaggt ctccacctgc agccggcggg cgccggcgac
     3781 aacgtgccgg ttggtgacga gatgccggcc gtcgacgacg aagcccgagc cgatggccac
     3841 gccgcaggcc ccgaccgcgc ggatgcgcag caccgactcc cgcgcgatcg tgaccgggtc
     3901 gtcgccctcg gtggcgccgg cgatgtcctc
//
LOCUS       DASTFV010000461         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1722370, whole genome shotgun sequence.
ACCESSION   DASTFV010000461 DASTFV010000000
VERSION     DASTFV010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1722370"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..338)
                     /locus_tag="VFP02_08520"
     CDS             complement(3..338)
                     /locus_tag="VFP02_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543110.1"
                     /translation="MLTRKAEPSDVEAIARNHQAAWQVGYRGLIADDLLDGLDLDARI
                     EHWRKAVTAGADGHAVLVIADDEASGATSPSGPPPGRGIRAARWSRSTWRPNVGATAT
                     GGRCSPGRP"
     gene            complement(420..1964)
                     /locus_tag="VFP02_08525"
     CDS             complement(420..1964)
                     /locus_tag="VFP02_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9543111.1"
                     /translation="MIDDIHVRRRAGVAGLLSAALALALTELAAGLSSQIPSPVAAVG
                     DVFIDGLPGFLVRAGIDQLGTSDKPFLITMILVIAGLLGAKIGTASLRRRWIAPAAFG
                     AAGLFGAWATARDPQSSAFVSILATLGPAMIGAAVLLKLIDLAEATQPTGEKAKASSA
                     ITNGEDRRRFLIGAAVTGAVALFGAGIGRRLKTRFSVESERSALTLPAVEPAASVSDL
                     GAEGLTPYLVPNADFYRIDTALTVPQIAPDDWTVRITGMVDHEIELTYADIQERAQVE
                     EIVTLSCVSNEVGGDLVGNARWQGILLADLLQEAGPQDGAEQVVGVSVDGFTAGFPLE
                     VALDGRPAMIAIGMNGEPLPVVHGFPARLVIPGLYGYVSATKWLSEIRLTTWDGFDGY
                     WIPRGWSKEGPIKTESRIDVPRGGSSLTAGTNAIAGVAWAPTRGIDKVEVQVDDGDWQ
                     EARLGDETTDLSWRQWVLEWDAPTGDHQVRVRATDGEGETQTDEIAPPAPSGATGWHT
                     ISVTVD"
BASE COUNT          285 a          735 c          760 g          283 t
ORIGIN      
        1 cctcacggcc ggcccggcga gcagcgcccg cccgtagccg tggccccaac gttcgggcgc
       61 cacgtagagc gagaccacct cgccgcccgg atcccccggc cgggtggcgg gcccgacggc
      121 gacgtggccc cagacgcctc gtcgtcggcg atgaccagaa cggcatggcc gtcggcaccg
      181 gccgtgaccg ccttccgcca gtgttcgatt cgggcgtcga ggtcgagacc gtcgaggagg
      241 tcgtcggcga tcagcccccg gtagccgacc tgccaggcgg cctggtggtt gcgggcgatc
      301 gcctcgacgt cgctcggctc ggccttcctg gtcagcaccg tcccgagtgt cgcccgcccc
      361 gctcggactg gcacccgact tgccggtcca ggccgggaat cgaaggccag gacgagaggt
      421 cagtcgacgg tgacgctgat ggtgtgccaa cccgtggcgc cgctgggggc ggggggagcg
      481 atctcgtcgg tctgggtctc gccctcgccg tcggtggccc ggacccggac ctggtggtcg
      541 ccggtggggg cgtcccactc gaggacccac tggcgccagc tcaggtcggt ggtctcgtcg
      601 ccgaggcggg cctcctgcca gtcgccgtcg tcgacctgga cctcgacctt gtcgatgccg
      661 cgggtcggcg cccaggccac gccggcgatg gcgttggtcc cggcggtcag cgacgacccg
      721 ccccggggga cgtcgatgcg gctctcggtc ttgatcgggc cctccttcga ccagccccgg
      781 gggatccagt agccgtcgaa gccgtcccac gtcgtgagcc ggatctcgct cagccacttg
      841 gtggccgaca cgtagccgta caggccgggg atgacgaggc gagccgggaa gccgtggacc
      901 accggcaggg gctcgccgtt catgccgatg gcgatcatcg ccggtcgacc gtcgagggcg
      961 acctcgaggg ggaacccggc ggtgaagccg tccaccgaga cgcccaccac ctgctccgcc
     1021 ccgtcctggg gcccggcctc ctgcagcagg tcggccagca ggatgccctg ccagcgggcg
     1081 ttgcccacca ggtcgccgcc gacctcgttg gacacacagg acagggtcac gatctcctcg
     1141 acctgggccc gctcctggat gtcggcgtag gtcagctcga tctcgtggtc gaccatgccg
     1201 gtgatgcgca ccgtccagtc gtcgggagcg atctggggca cggtcagcgc cgtgtcgatc
     1261 cggtagaagt ccgcgttggg gacgagatag ggggtcagcc cctcggcgcc gagatcgctg
     1321 acgctggctg cgggctcgac ggcgggcagg gtcaacgccg accgctccga ctcgaccgag
     1381 aaccgggtct tcaggcggcg cccgatgccg gcgccgaaga gggcgaccgc gccggtcacc
     1441 gcggcgccga tcaggaaccg gcgccgatcc tcgccgttgg tgatggccga cgaggccttg
     1501 gccttctccc cggtcggctg ggtggcctcg gccaggtcga tcagcttgag caggaccgcg
     1561 gcgccgatca tcgccgggcc gagcgtggcc aggatcgaca cgaaggcact ggattgcggg
     1621 tcccgggccg tggcccaggc gccgaagaga ccggcggcac cgaaggcggc gggggcgatc
     1681 cagcgccggc ggagcgaggc ggtaccgatc ttggccccga gcaggccggc gatcaccagg
     1741 atcatggtga tcaggaaggg cttgtccgac gtgcccagct ggtcgatgcc cgcccgcacc
     1801 aggaacccgg gcaggccgtc gatgaacaca tcgcccacgg cggcgacggg ggaggggatc
     1861 tggctcgaca gcccggcggc caactccgtg agcgccaggg ccagggccgc actcagcagc
     1921 cccgccacgc ccgcgcgtcg tcggacgtgg atgtcatcga tcacggacct tccacgctag
     1981 gaggtccctg agagttccct caggcccagc cgccggcgta ggcggtgaac tgcccggtcg
     2041 tgaagcggct ggtgccgtcg acg
//
LOCUS       DASTFV010000462         4323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1723348, whole genome shotgun sequence.
ACCESSION   DASTFV010000462 DASTFV010000000
VERSION     DASTFV010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723348"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VFP02_08530"
     CDS             <1..560
                     /locus_tag="VFP02_08530"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9543112.1"
                     /translation="GYTLVTGPGDDDLVAHAEAEGLWAFADAPEMVCRDRLADVDPPA
                     GIELRRATDEAGIADFVAVGSAAYPSLGMPDTAMGEAIDRYDRVLEPHVAVVVAYDGD
                     APVAAAQAMLSHGIAGVYWVGTLEAARGKGLGEAVCRWVTNWAFDQGAAAQSLQASTM
                     GEPIYARMGYETIHRYQSWFRSSDR"
     gene            complement(567..1292)
                     /locus_tag="VFP02_08535"
     CDS             complement(567..1292)
                     /locus_tag="VFP02_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9543113.1"
                     /translation="MRRRFSLLLVLVLATTAAVGLAPRAGAGPGSCVEVRGRVRDAAT
                     GLPLAEVTSVELADAGGPIDGFGTDENSRYSICVVPGTYHIKFVADDYRPEWYHDQPN
                     FASATPITVAGPDPVVVNEALVPRGRVIAGRVTNASGVPKFASVGIWRRTSTGWHSID
                     GIGNDEATGWYSFVVPGPGRYRVSAGVDHHKARFATSATHLSAGRIITIGVPTTFVND
                     VHVRVPYCPGPTDDICVPAGFLT"
     gene            complement(1303..2487)
                     /locus_tag="VFP02_08540"
     CDS             complement(1303..2487)
                     /locus_tag="VFP02_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543114.1"
                     /translation="MDELTRADLCGRTERWFVQRGLPHFIDEYRATTDVWTRAAPFLT
                     LVFLAELAFSLDEDREGLDELLPLLGGVAIFVTGVVVVNRLRGRRPLQRPDRVGTPEL
                     SLFVIAPALLPALFSNDHLTDALGAVLLNLVVLAVTYAVTSYGLVPMTRWALGQMFRQ
                     LANVADLMVRSLPLLLLFATFLFLNTEIWQVAAELEAPFLLMVLGMLFGAGVIFLVLR
                     QRREVELVSHFRSWPEALECSGSSPVAVVAVDGLVEPPDPQPLGRRARINIALVLVFS
                     QGVQIILVATVIGLFYVGFGLVAVRETTMASWVGAPLDVIAEVTVFGHPVVLTWDLIR
                     VAGLVAAFSALQFTFSALTDQTYREEFFEGMLSEMRESLATRAVYLEALVGAHRTEGV
                     AD"
     gene            2519..3283
                     /locus_tag="VFP02_08545"
     CDS             2519..3283
                     /locus_tag="VFP02_08545"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9543115.1"
                     /translation="MGDVLVTGGTGVLGTRVVERLLAGGHSVRITSRDGAAVAPDGVS
                     VHQADLKSGQGLVEAVAGVDTIVHAATALKAKKKVEVGGAGRLLEAIGDARPHLLYVS
                     IVGVDRHPYSYYRAKWAAEQVLEAGEVPLTIQRATQFHDLLHRLLSMPISGLPFGATF
                     QPIDVSEVAGRLVELVDTGPAGRAEDIGGPEVLDIEDLTGQYAEVLGTKPRCFTLPAA
                     GKTLQAFGKGIQTTPDHRYGTITWRQFLETKAAAGP"
     gene            complement(3290..>4323)
                     /locus_tag="VFP02_08550"
     CDS             complement(3290..>4323)
                     /locus_tag="VFP02_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543116.1"
                     /translation="EDEDLTDVLTRLGITTLADLAALPRPDLVARFGWPGERAHRLAS
                     GDDARLPDTRPPPPDLIARVELDPPAERVDAAAFAAKRLADDLHERLAGRGLSCAQVL
                     VTAETEHGEVHERCWRHEGALTAPAMAERVRWQLEGWLNSSAASRPTSGIALLRLEPI
                     EVGAARGRQLGFWGGESAAAERAARALARVDGLLGPGAATVPERRGGRSPAEAIALVP
                     ADAVDLTERSLVTDAAVAPWPGRLPTPLPARVLATPVRAEVVDRHDRTVGVSGRGMAT
                     AAPARVRIGTGADQAVAGWAGPWPVDERWWDTANHRRRARFQVLTAEGTALLLSVEEG
                     RWWLEATYD"
BASE COUNT          633 a         1518 c         1649 g          523 t
ORIGIN      
        1 ggggctacac gctggtgacc gggccgggtg atgacgacct cgtcgcccac gccgaggccg
       61 agggcctctg ggcgttcgcg gacgcgccgg agatggtctg tcgggacagg ctggctgacg
      121 tcgacccacc agcgggcatc gagctgcgcc gagccaccga cgaggctggg atcgccgact
      181 tcgtggccgt cggctcggcc gcttacccgt ccctgggcat gcccgacacg gccatgggcg
      241 aagccatcga ccgctacgac cgggtcctcg agccccacgt ggcggtggtc gtcgcctacg
      301 acggcgatgc gccggtcgcc gccgcccagg cgatgctcag ccacggcatc gccggcgtct
      361 actgggtcgg cacgctcgag gccgcccggg gcaagggcct gggtgaggcg gtctgccgct
      421 gggtcaccaa ctgggccttc gaccaggggg cggcggccca gagcctgcag gcctcgacga
      481 tgggagagcc gatctacgcc cgcatgggtt acgagaccat ccaccgctac cagagctggt
      541 tccggtcctc ggaccggtga tggagctcag gtgaggaagc ccgccggcac gcagatgtcg
      601 tcggtgggcc cggggcagta cggcactcgg acgtgcacgt cgttgacgaa cgtcgtcggc
      661 accccgatgg tgatgatgcg gcccgcgctc aggtgcgtgg cactggtggc gaagcgagcc
      721 ttgtggtggt ccacgccggc cgagacccgg tagcgcccgg gcccgggcac cacgaaggag
      781 taccagccgg tggcctcgtc gttgccgatg ccgtcgatcg agtgccaccc cgtgctggtc
      841 cggcgccaga tcccgacgct ggcgaacttg ggcaccccac tggcgttggt gacccggccg
      901 gcgatgaccc ggccccgggg cacgagcgcc tcgttgacca cgacggggtc cgggcccgcg
      961 accgtgatcg gcgtggccga ggcgaagttg ggctggtcgt ggtaccactc cggccggtag
     1021 tcgtcggcca cgaacttgat gtggtacgtg cccggcacca cgcagatcga gtaacggctg
     1081 ttctcgtccg tgccgaagcc gtcgatgggc ccaccggcgt ccgccagctc gacgctcgtg
     1141 acctcggcca gcggcagccc ggtggccgcg tcccggaccc ggccgcggac ttcgacacac
     1201 gacccgggac cggcgccggc gcgcggggcg agcccgacgg cggcggtggt cgcgaggacg
     1261 aggacgagga gcaggctgaa gcgacgtcgc atcggcggga cgctagtccg cgaccccttc
     1321 ggttcggtgg gcaccgacca gggcctcgag gtacaccgct cgggtggcca gcgactcgcg
     1381 catctccgag agcatcccct cgaagaactc ctcgcggtag gtctggtcgg tcaaggccga
     1441 gaaggtgaac tgcagggccg agaaggcggc gaccaggccc gcgacccgga tcaggtccca
     1501 ggtcaggacg accgggtggc cgaagaccgt gacctcggcg atgacgtcca gcggggcgcc
     1561 gacccaggag gccatggtgg tctcccgcac ggccaccagg ccgaagccga cgtagaagag
     1621 cccgatcacg gtcgccacca ggatgatctg cacgccctgg ctgaagacca gcacgagggc
     1681 gatgttgatc cgggcccggc ggccgagggg ctgggggtcg ggcggctcga ccaggccgtc
     1741 gacggccacc accgcgaccg gcgaggaccc cgagcactcg agggcctcgg gccagctgcg
     1801 gaagtgcgag acgagctcca cctcgcgccg ttggcggagc acgaggaaga tgaccccggc
     1861 gccgaagagc atgccgagga ccatgagcag gaacggggcc tccagctccg cggccacctg
     1921 ccagatctcg gtgttgagga acaggaaggt ggcgaagagc aggagcaatg gcagggagcg
     1981 gaccatcagg tccgccacgt tggccagctg gcggaacatc tggccgaggg cccagcgggt
     2041 catgggcacc aggccgtagc tggtcacggc gtacgtcacg gccaggacca ccaggttgag
     2101 caggacggcg ccgagcgcgt ccgtcaggtg gtcattgctg aagagggcgg gcagcagcgc
     2161 cggggcgatg acgaacaggc tcagctcggg cgtgcccacc cggtcggggc gctggagggg
     2221 acggcggccg cgcagccggt tcaccacgac gacgccggtc acgaagatgg ccacgccgcc
     2281 gaggaggggc agcagctcgt ccaggccctc acggtcctcg tcgaggctga aggcgagctc
     2341 ggccaggaag acgagggtca ggaacggtgc ggcccgggtc cagacgtcgg tggtggcccg
     2401 gtactcgtcg atgaagtggg gcaggccccg ctggacgaac caccgctcgg tccggccaca
     2461 gaggtcggcc cgcgtgagtt catccattcg ccagagtctg gcccgtaggt tgggagccat
     2521 gggagatgtc ttggtcaccg gcgggacggg cgtgctcggc acccgcgtcg tggagcgcct
     2581 gctggccggc ggccactccg tgcggatcac ctcgcgggac ggcgccgcgg tggcgcccga
     2641 cggggtgagc gtgcaccagg ccgacctgaa gtcggggcag ggactggtcg aggccgtggc
     2701 cggggtcgac accatcgtcc acgcggccac ggcgctgaag gccaagaaga aggtcgaggt
     2761 cggtggcgcc gggcgactgc tggaggccat cggcgacgcc cgcccccacc tgctgtacgt
     2821 gtccatcgtg ggcgtcgacc gccaccccta cagctactac cgggccaagt gggcggccga
     2881 gcaggtcctc gaggccggcg aggtgccgct caccatccag cgggccaccc agttccacga
     2941 cctgctgcac cggctgctga gcatgccgat ctccgggttg cccttcggcg ccaccttcca
     3001 gcccatcgac gtgagcgagg tggcgggccg tctggtcgag ctggtcgaca ccggcccggc
     3061 gggtcgggcc gaggacatcg gcggcccgga ggtgctggac atcgaggacc tgaccggcca
     3121 gtacgccgag gtgctgggga ccaaaccccg ctgcttcacc cttcccgccg cgggcaagac
     3181 cctgcaggcc ttcggcaagg gcatccagac cacccccgac cacaggtacg gcaccatcac
     3241 ctggcgccag ttcctggaga ccaaggccgc cgccgggccc tgagcccggt cagtcgtagg
     3301 tggcctccag ccaccaccgg ccctcctcga cggacaggag cagggccgtg ccctcggcgg
     3361 tcaggacctg gaagcgggcc cggcggcggt ggttggcggt gtcccaccag cgctcgtcca
     3421 ccggccaggg cccggcccag cccgccaccg cctggtcggc gccggtgccg atccgcaccc
     3481 gggccggggc cgcggtggcc atgccccggc cgctgacccc caccgtccgg tcgtggcggt
     3541 ccaccacctc ggcccgcacc ggggtggcca ggacccgggc cggaagggga gtgggcaggc
     3601 gaccgggcca aggggcgacg gcggcgtcgg tgaccaggga gcgctcggtg aggtccacgg
     3661 catcggccgg gaccagggcg atggcctcgg ccggactgcg cccgccccgc cgctcgggga
     3721 cggtggcggc ccccgggccc aggaggccgt ccacccgggc cagggcccgc gccgcccgct
     3781 ccgccgccgc cgactcgccg ccccagaagc cgagctgacg gccccgggcc gcccccacct
     3841 cgatgggctc caggcggagg agggcgatcc cgctggtggg acggctggcg gcgctgctgt
     3901 tgagccaacc ctccagttgc cagcgcaccc gctcggccat ggccggggcg gtgagggccc
     3961 cctcgtggcg ccagcagcgc tcgtggacct cgccgtgctc ggtctcggcg gtgaccagga
     4021 cctgggcaca ggacaacccc cgtccggcca ggcgctcgtg gaggtcgtcg gccaggcgct
     4081 tggccgcgaa ggcggcggcg tcgacccgct cggccggggg gtccaactcg acgcgggcga
     4141 tcaggtcggg tgggggcggc cgggtgtcgg gcaggcgggc gtcgtcgccg gaggccaggc
     4201 ggtgggcccg ctcccccggc cagccgaagc gggccaccag gtcggggcgg ggcagggcag
     4261 cgaggtcggc cagggtggtg atgcccaggc gggtgagcac gtcggtcaag tcctcgtcct
     4321 cca
//
LOCUS       DASTFV010000463         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1725018, whole genome shotgun sequence.
ACCESSION   DASTFV010000463 DASTFV010000000
VERSION     DASTFV010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725018"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VFP02_08555"
     CDS             <1..803
                     /locus_tag="VFP02_08555"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9543117.1"
                     /translation="YEALANDDDVDVVYVATPQSRHADDACLFLDHGKHVLCEKPLAM
                     TEAEVLRMTASAHLAQRFLMEAIWSRFLPSYVTLWELLLHGRIGEPLFVEADFGFRMP
                     VDPAHRLFDPARGGGALLDLGVYPLQLASFVLGAPDRVAASGHMGTTGVDETVAATLH
                     HPGGKLAVVKASIRVNLACTARIVGTEGSIELPAFMHCPDALTVRDAFGQAEVIATPI
                     EGEGLRYQVDEVHRCLAEGLGESRVMGLHESAQIAATMDTIREQIDPA"
     gene            complement(880..1716)
                     /locus_tag="VFP02_08560"
     CDS             complement(880..1716)
                     /locus_tag="VFP02_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9543118.1"
                     /translation="MEHVRIDEVEDGIVVLTIDRQDRRNALSNKVLAELHEAVDTIGS
                     DPAIRVVVLTGAGAGFSAGADMKGGPEEMQGLGGTPIGAVLEAAQNDVVRTFASQEHM
                     AGLFEKVHRLRQPVIAAVNGAAVGGGFALALACDIRYAAESASFGAVFIKRGVSACDM
                     GTSYHLPRLVGASRSAELLLTGRTFDATEARDMGLVLDVVPDGEVVRRALVTARQIAE
                     NGPLAVWMTKETMWQTVDSPSLRHALDLENRTQIMCTGTGELAEAFAAFREGRTPTWG
                     PL"
     gene            complement(1729..2205)
                     /locus_tag="VFP02_08565"
     CDS             complement(1729..2205)
                     /locus_tag="VFP02_08565"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET9543119.1"
                     /translation="MTGTPVLDIVTSRLTLRHLPLAVMEERAASDFDGSGWLYELRSG
                     QLREDPGFAPWSLRDVILTGTGETIGHGGFHEAPVEGVAEIGYRIHTSWQGRGFATEV
                     TEAMMAFGRAHGATAFLLSISPDNAASLAIAAKLGFARIGEQMDEVDGLEWVFRLG"
BASE COUNT          328 a          854 c          810 g          334 t
ORIGIN      
        1 actacgaggc cctggccaac gacgacgacg tcgacgtcgt ctacgtcgcc accccgcagt
       61 cccgccacgc cgacgacgcc tgcctcttcc tcgaccacgg caagcacgtg ctgtgcgaga
      121 agccgttggc catgaccgag gccgaggtgc tgcgcatgac ggccagcgcc cacctggccc
      181 agcgcttcct catggaggcc atctggagcc ggttcctccc ctcctacgtc acgctgtggg
      241 agctgctgct gcacggtcgc atcggtgagc cgctgttcgt cgaggccgac ttcggcttcc
      301 gcatgcccgt cgacccggcc caccgcctct tcgacccggc ccgcggcggc ggcgccctgc
      361 tcgacctcgg cgtctatccg ctgcagctgg cgtcgttcgt gctcggagcc cccgaccggg
      421 tggcggccag cgggcacatg ggcaccaccg gcgtcgacga gaccgtggcc gccaccctgc
      481 accacccggg cgggaagctg gccgtggtga aggcctcgat ccgggtgaac ctggcctgca
      541 ccgcccgcat cgtgggcacc gagggcagca tcgagctgcc cgcgttcatg cactgccccg
      601 acgccctcac cgtacgggac gccttcggcc aggctgaggt catcgcgacc cccatcgagg
      661 gtgagggcct gcgctaccag gtcgacgagg tccaccgctg cctcgccgaa gggctgggcg
      721 agagccgggt catgggcctg cacgagtcgg cccagatcgc cgccaccatg gacaccatcc
      781 gggagcagat cgacccggcc tgatcacccg ttctggcctc cgatcctccg gcctgggccg
      841 gaggatcgga ggccagaacg agggaagcgg tcgccgtcgt cagagcgggc cccacgtggg
      901 ggtacggccc tcccggaagg cggcgaaggc ctcggccagc tcaccggtgc cggtgcacat
      961 gatctgggtg cggttctcga ggtcgagggc gtggcggagg ctgggcgagt cgaccgtctg
     1021 ccacatggtc tccttggtca tccagacggc cagcggcccg ttctcggcga tctggcgggc
     1081 ggtgaccagg gcccgccgga cgacctcacc gtcggggacc acatccagga cgaggcccat
     1141 gtcccgggcc tcggtggcgt cgaaggtccg gccggtcagg agcagctcgg ccgagcggga
     1201 ggccccgacc agtcggggca ggtggtacga ggtgcccatg tcgcaggccg acacgccccg
     1261 cttgatgaag acggcgccga aggaggccga ctcggccgcg tagcggatgt cgcaggccag
     1321 ggccagggcg aagccgccac ccacggccgc gccgttgacg gcggcgatga ccggttggcg
     1381 gagccggtgg accttctcga acaggccggc catgtgctcc tggctggcga aggtccggac
     1441 cacgtcgttc tgggcggcct cgaggacggc gccgatgggc gtgccgccaa ggccctgcat
     1501 ctcctcgggc ccgcccttca tgtcggcccc ggcggagaag cccgcccccg ccccggtcag
     1561 gacgacgaca cggatggcgg ggtccgaccc gatggtgtcc accgcctcgt gcaactcggc
     1621 cagcaccttg ttgctgaggg cgttgcggcg gtcctggcgg tcgatggtca ggacgacgat
     1681 gccgtcctcc acctcgtcga tcctcacgtg ctccatgccg cgatcctgtc acccgaggcg
     1741 gaagacccac tcgaggccgt cgacctcgtc catctgctcg ccgatccggg cgaacccgag
     1801 cttcgccgcg atggccagcg aggcggcgtt gtccggggag atcgagagca ggaacgccgt
     1861 ggccccgtgg gcccgcccga aggccatcat cgcctcggtc acctcggtgg cgaagccgcg
     1921 tccctgccac gacgtgtgga tccggtagcc gatctcggcg acgccctcta ccggcgcctc
     1981 gtggaacccg ccgtgcccga tggtctcccc cgtgcccgtc aggatcacgt cccgcaacga
     2041 ccacggcgcg aagcccggat cctcccggag ctgacccgac cgcagctcgt acagccaccc
     2101 cgacccgtcg aagtccgagg ccgcccgctc ctccatgacc gccaggggca gatgacgcag
     2161 cgtcaaccgg gacgtgacga tgtcgaggac gggtgtgccg gtcacgccgg gatcgtggca
     2221 cctacggtgc tcgtgtgcga tggcattggc tggtcggctc ggcggtgctc gccctcgtgg
     2281 cttcgggttg tggcggcggg aacgacccga ccccggtctt cggggc
//
LOCUS       DASTFV010000464         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1726228, whole genome shotgun sequence.
ACCESSION   DASTFV010000464 DASTFV010000000
VERSION     DASTFV010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726228"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1246)
                     /locus_tag="VFP02_08570"
     CDS             complement(50..1246)
                     /locus_tag="VFP02_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HET9543120.1"
                     /translation="MGSHGIKDQVAIIGMGCTRFGERWDTGTDDMLIESSNEAYESAG
                     ITPADVDAYWLGTAMSGTSGMDLAGPLKLVDKPVSLVQNFCATGSEALRQACYAVAAG
                     AYDVAMAIGVEKVKDGGYQGLSGAAKIPTDGTGRTLTAAAMYAMILPAYAKRYGVDPD
                     ELRMVVAKIAEKNHYNGARNERAQFRRETSAEQICAMPTVAGQLSVFDCSGVADGSAA
                     AIVCRAEDAHKYTDKPLYVKGLGFVAGTKAGNLDPAYDYTHFPEAAKAAADAYAQAGI
                     TDPRAQLAMAEVHDCFTPTELVLMEDLGFAERGMAWKEVLAGTFDLEGDLPVNPDGGL
                     KSFGHPVGASGLRMHFENWLQLRGEAPEERRIKTADRGLGLVHNLGGYPGEMLSFVSV
                     LGTELG"
     gene            complement(1248..>2513)
                     /locus_tag="VFP02_08575"
     CDS             complement(1248..>2513)
                     /locus_tag="VFP02_08575"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HET9543121.1"
                     /translation="LRGAPGAAPDRLWFATSAPAYLDKTNATAIHAALRLDSGALAMD
                     AGGAIRSGVGALRAALDSSGTTLVVSADLRAGRPSSAEEASGGDGASAALVGEGDVIA
                     EYLGGASVTDEFTERWRGPGDRHTRSWEDRFGESVYPDLGRQAWEAALKAASLEADAV
                     RRVAVHSQNARAAGRMAKALGGVQVVDDLSATVGNTATAHAGLLLATMVESAEPGEVL
                     ALVSLVDGADVLLFRATDAVSGAAVRPVAGQVAAAADLPYGTFLAWRGEIELEPPNRP
                     EPGRASTAVAHRSKDWKFAFEGSRDKASGALHLPPARVSFTNAHQDDMDPAPMADVEA
                     TIATFTVDRMAYSPSPPIVFGILDFDGGGRFPCELTDCSEDDVHIGDRVEMTFRVLGT
                     QDGIHNYFWKARLLPRDEPSGEEPKAQED"
BASE COUNT          343 a          858 c          942 g          370 t
ORIGIN      
        1 aagggccggg tctcgggctc gatggtgggg agatcgaacc ggacctcgct caaccgagct
       61 ccgtgcccag gacggagacg aacgagagca tctcgccggg gtagccgccg aggttgtgga
      121 ccaggccgag accccggtcg gcggtcttga tccggcgctc ctccggggcc tcaccccgga
      181 gctgcagcca gttctcgaag tgcatgcgca ggccggaggc gccgacggga tgaccgaagg
      241 acttcaggcc accgtcgggg ttgaccggga ggtcgccctc aaggtcgaag gtgccggcca
      301 gcacctcctt ccaggccatg ccccgctcgg cgaagcccag gtcctccatg aggaccagct
      361 cggtcggcgt gaagcagtcg tggacctcgg ccatggccag ctgcgcgcgg gggtcggtga
      421 tgccggcctg cgcataagca tcggccgcgg ccttcgccgc ctcggggaag tgcgtgtagt
      481 cgtaggccgg gtcgagattg cccgctttgg tgccggcgac gaagccgagg cccttcacat
      541 acagcggctt gtccgtgtac ttgtgcgcgt cctcggcccg gcagacgatc gccgccgccg
      601 acccgtcggc cacgccgctg cagtcgaaga ccgacagctg gccggcgacg gtcggcatgg
      661 cgcagatctg ctcggcgctg gtctcccgcc ggaactgggc ccgttcgttg cgggcgccgt
      721 tgtagtggtt cttctcggcg atcttggcca cgaccatgcg cagctcgtcg gggtcgacgc
      781 cgtagcgctt ggcgtaggcg ggcaggatca tggcgtacat ggccgccgcg gtgagcgtcc
      841 ggccggtgcc gtcggtcggg atcttggccg cccccgacag gccctggtag ccgccgtcct
      901 tcaccttctc gacaccgatg gccatggcca cgtcgtaggc cccggcggcc acggcgtagc
      961 aggcctggcg cagggcctcg gagcccgtgg cgcagaagtt ctgcacgagg ctgaccggct
     1021 tgtcgaccag cttcagcgga ccagccaggt ccatgccact ggtgccggac atggcggtgc
     1081 ccagccagta ggcgtcgacg tccgcgggcg tgatgcccgc cgactcgtag gcctcgttcg
     1141 acgactcgat gagcatgtcg tcggtcccgg tgtcccagcg ctcaccgaag cgggtgcagc
     1201 ccatgccgat gatggcgacc tggtccttga tgccgtgcga acccatgtca gtcctcctgt
     1261 gcctttggtt cttcgcctga cggctcatca cgaggaagga gccgggcctt ccagaagtag
     1321 ttgtggatgc cgtcctgggt gcccaggacg cggaacgtca tctccacccg gtcgccgatg
     1381 tgcacatcgt cttcggagca gtcggtcagc tcgcagggga agcgcccgcc cccgtcgaag
     1441 tcgaggatgc cgaagacgat cggcgggctg ggcgagtagg ccatgcggtc gaccgtgaag
     1501 gtggcgatgg tggcctcgac gtcggccatc ggggccgggt ccatgtcgtc ctgatgggcg
     1561 ttggtgaacg acacccgggc cggcggcagg tgcagggcgc cggaggcctt gtccctcgag
     1621 ccctcaaagg cgaacttcca gtccttggac cggtgggcca cggcggtgga ggcccggccg
     1681 ggctcgggcc ggttgggcgg ctccagctcg atctcgcccc gccaggccag gaaggtgccg
     1741 tagggcaggt cggccgcggc cgccacctgg ccggcgacgg ggcggacggc ggcacccgac
     1801 acggcgtcgg tggcccggaa gaggaggacg tcggcgccgt cgacgagcga gaccagggcc
     1861 agcacctccc ctggctcggc ggactcgacc atggtggcca gcaggaggcc cgcgtgggcg
     1921 gtggcggtgt tgccgacggt ggcgctcagg tcgtccacga cctggacccc gccgagggcc
     1981 ttggccatgc ggccggcggc ccgggcgttc tggctgtgga cggcgaccct cctcacggcg
     2041 tcggcctcca gcgaagcagc cttgagggcg gcctcccagg cctggcggcc gaggtcgggg
     2101 tagacggact cgccgaatcg gtcctcccac gagcgggtgt ggcgatcgcc cgggccccgc
     2161 caccgctcgg tgaactcgtc ggtgaccgag gcgccgccca ggtactcggc gatcacgtcg
     2221 ccctcgccga cgagggcggc agaggcgccg tcgccgccag aggcctcctc ggcactggac
     2281 ggccggccgg cgcgcaggtc ggcactgacc acgagcgtgg tcccgctcga gtcgagcgcg
     2341 gcgcgcaggg cgccgacgcc ggaccggatg gcgccgccgg cgtccatggc cagcgcgccg
     2401 gaatccagcc gaagtgcggc gtggatcgcc gtggcgttgg tcttgtccag gtaggcgggg
     2461 gccgacgtgg cgaaccacag acggtccggc gcggcgccgg gagcgcccct gag
//
LOCUS       DASTFV010000465         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1727364, whole genome shotgun sequence.
ACCESSION   DASTFV010000465 DASTFV010000000
VERSION     DASTFV010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727364"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="VFP02_08580"
     CDS             complement(<1..1081)
                     /locus_tag="VFP02_08580"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET9543122.1"
                     /translation="MLRWAVDHRGAITALTDALVWPLALAYSCWLRYEFTLAPVNGLK
                     LAFLALFAAGFQVGLGRWQGLYTGRWRFGSFEEVANLFGTVGITTGVVFVVNLIPDPR
                     LVPSGAALASGLVALVMMGGVRYGWRLYLEHLRRPDQERANRTIVYGAGDAGHQLVRA
                     MTSDPRSPLLPVAVIDDDPRQQQHRLARHVAVVGTGEDLAAVAARYDAEIVVLAIPSA
                     TSELVRSVVAEGEQAGLDVRVLPPLWELAENKVRVSDVRAVTITDLLGRHELDLDSAA
                     IAGYLADRRVLVTGAGGSIGSELCRQIAGYDPAELIMLDRDESSLQALQLGLDGEGRV
                     DDERLVVADIRDADRMFEVFRRFRPE"
     gene            complement(1298..1780)
                     /locus_tag="VFP02_08585"
     CDS             complement(1298..1780)
                     /locus_tag="VFP02_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET9543123.1"
                     /translation="MPDLEDLYREIILDHYRSPRNRGELESPPAHRVEGFNPLCGDEV
                     VVYLDVDDDGNVRELKISGQGCSISQSSASMMSAAVNGKSVAEARDMTRAFKEMMSIH
                     EHEIAGSDGEPDAESPEVNLGDLAALRGVVKFPVRIKCATLSWNTLTQGLDEIEAERS
                     "
     gene            complement(1795..>2161)
                     /locus_tag="VFP02_08590"
     CDS             complement(1795..>2161)
                     /locus_tag="VFP02_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9543124.1"
                     /translation="DYLEGLDMAEVREHEVTLTAYALRSLTERFGDEITIHGPTEPGE
                     RGGVLSFAVRGLHAHDVSQVLDQDAVCVRAGHHCAKPLMRHLDVSATARASLYVYNDT
                     DDVDRLGDAIAHTVEFFAL"
BASE COUNT          330 a          722 c          781 g          328 t
ORIGIN      
        1 cctcgggtcg gaagcgccgg aacacctcga acatccggtc ggcgtcacgg atgtcggcca
       61 ccaccagccg ctcgtcgtcc acccgacctt cgccgtcgag gcccagttgc agcgcttgta
      121 gggacgactc gtcgcgatcg agcatgatca actcggccgg gtcatacccg gcgatctggc
      181 gacagagctc ggagccgatg gagcccccgg ccccggtgac cagcacccgg cggtcggcga
      241 ggtacccggc gatggcggcg ctgtccaggt ccagctcgtg gcgaccgagc aggtcggtga
      301 tggtcacggc ccggacgtcg ctcacccgga ccttgttctc ggccagctcc cacaacgggg
      361 gaaggacccg cacgtccagg cccgcctgct caccttcggc gacgacggac cgcacgagct
      421 cgctggtggc actgggtatg gccaggacga cgatctcggc gtcgtagcgg gccgccactg
      481 cagccaggtc ctcccccgtg ccgacgacgg cgacgtggcg ggccagccgg tgctgctgct
      541 gccggggatc gtcgtcgatg actgcaaccg ggagcagggg gctgcggggg tcgctggtca
      601 tcgcccgcac cagttgatgg ccggcatcgc ctgcgccgta gacgatggtc cggttggccc
      661 gctcctggtc cgggcgccgc aggtgctcga ggtacagccg ccagccatag cggacgccgc
      721 ccatcatgac gagggcgaca agaccggagg ccagggcggc gccgctgggg acgaggcggg
      781 gatccgggat caggttgacg acgaacacca cgccggtggt gatcccgacg gtcccgaaga
      841 ggttggccac ttcctcgaag ctgccgaagc gccatcggcc ggtgtacagc ccctgccacc
      901 gtccgagccc gacctggaat ccggccgcga agagagcgag gaaagcgagt ttgaggccgt
      961 tcacgggggc aagggtgaac tcgtaccgca accagcacga gtaggcgaga gccaggggcc
     1021 acaccagggc gtcggtcagg gccgtgatcg cgccccggtg gtctacggcc caccggagca
     1081 cccgttggcg gcgctccgtt gtcggcgcag gtctcggacc ctcagtaacc ctgtccgccg
     1141 ccattgccct tcagtccgct cgtcgtccga ctcacagcaa tcccagcata cggagtgggg
     1201 gccagcggct gtggaaccct ccggcgggct cggtgtcgcc ctcgctctgg tcggaccgac
     1261 ctccgcgggg tgagtggacg aggaaacgag aacagctcta cgagcgctcg gcctcgatct
     1321 cgtccaagcc ctgggtgagg gtgttccagg agagggtggc gcacttgatg cgcaccggga
     1381 acttcacgac gccccggagg gcggccaggt cgcccaggtt gacctcgggg gactcggcgt
     1441 cgggctcgcc gtcgctgccg gcgatctcgt gctcgtggat cgacatcatc tccttgaagg
     1501 cccgggtcat gtcccgggcc tcggccacgg acttgccgtt gacggcggcg ctcatcatcg
     1561 aggccgacga ctggctgatc gagcagccct ggccggagat cttcagctcg cgcacgttcc
     1621 cgtcgtcgtc gacgtcgagg tagaccacga cctcgtcacc gcacaggggg ttgaagccct
     1681 cgacccgatg ggcgggtggg gactccagtt cgccccggtt ccgggggctg cggtagtggt
     1741 cgaggatgat ctcgcggtag aggtcttcga ggtctggcac ggtgactccg gacgctacag
     1801 ggcgaagaac tcgacggtgt gggcgatggc gtcgcccaat cggtccacgt cgtcggtgtc
     1861 gttgtagacg tacaacgagg cccgagccgt ggccgacacg tcgaggtgtc gcatcagtgg
     1921 tttggcgcag tggtggccgg cccgcacgca caccgcgtcc tggtcgagga cctgcgacac
     1981 gtcgtgggcg tggaggccgc gcaccgcgaa ggagagcacg ccgccccgct ccccgggctc
     2041 ggtggggccg tggatggtga tctcgtcgcc gaagcgctcg gtgagggagc gcagggcgta
     2101 ggcggtgagg gtgacctcgt gctcccgcac ctcggccatg tcgaggccct cgaggtagtc
     2161 g
//
LOCUS       DASTFV010000466         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1730743, whole genome shotgun sequence.
ACCESSION   DASTFV010000466 DASTFV010000000
VERSION     DASTFV010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730743"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VFP02_08595"
     CDS             complement(<1..943)
                     /locus_tag="VFP02_08595"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET9543125.1"
                     /translation="MSDPVIQTVELGAAPWPTIDPFLFCVHHRDAYPPGDERMAPQAS
                     LDGRDLGMDFSGRDGWSMYHGLVVPGFPQHPHRGFETVTYVRRGYVDHSDSLGATARF
                     GRGDVQWLTAGGGIVHAEMFPLVEEEADNPTELFQIWLNLPGEDKLVDPHFSMLWDED
                     IPRVVATDADGRSVEITVIAGALAGEQPAPPPPRSWASRTDTEVAIWHLRFEPGSSWV
                     LPPAGPETVRMLYVFEGAGLDVAGHVLGARSGAAIEAGEPVELTAGDEPIEALILQGR
                     PIGEPVAQYGPFVLNDKAGIEQAFADYRRTGFGGWPFP"
     gene            1101..1337
                     /locus_tag="VFP02_08600"
     CDS             1101..1337
                     /locus_tag="VFP02_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543126.1"
                     /translation="MSWITGSEGDSIHSGSSAVTGPSGCCHAVDPDDGATACGTIIRS
                     LQLWDQVPWIRARMAGGELCPACMAVTEEHQVPA"
     gene            complement(1362..>2189)
                     /locus_tag="VFP02_08605"
     CDS             complement(1362..>2189)
                     /locus_tag="VFP02_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family zinc-binding domain-containing
                     protein"
                     /protein_id="HET9543127.1"
                     /translation="EGAVARQGGKWSRTGRDWAYDADRVERVTAERRREQAAMDDFGS
                     TTDCLMQLLLAELDDPASGPCGRCGNCAPVAEEPLDPAEVARAIEFVRGQPLLVDPRR
                     QWPSGLDEVRGKIPAELQVEEGRALCLWTDGGWGTRVRAGRKTGAYADELVGAAARLV
                     DSWAPTPAPTWVTAVPSDDRPEVAGFAEALADAVGLPFVAAVTTARAKAAQATRENSA
                     QQVLNVFGAFAVDGDAPPGPVLLVDDTASSRWTVTVVGAALREQGTDAVFPLVLAQR"
BASE COUNT          295 a          783 c          814 g          297 t
ORIGIN      
        1 cggggaaggg ccaaccgccg aagccggtgc ggcggtagtc ggcgaaggcc tgctcgatgc
       61 cggccttgtc gttcaggacg aacgggccgt attgggccac gggctcgccg atgggccgac
      121 cctgcaggat cagggcctcg atgggctcgt ccccggccgt caactcgacc ggctcgccgg
      181 cctcgatggc ggcgccgctg cgagcgccga ggacgtggcc ggcgacgtcg aggcccgcac
      241 cctcgaacac gtagagcatg cggacggtct ccggcccggc cggcggcagg acccaggacg
      301 agcccggctc gaaccgcagg tgccagatgg ccacttcggt gtcggtccgg ctggcccagg
      361 accggggtgg gggcggtgcc ggctgctcgc ccgccagcgc gcccgcgatc acggtgatct
      421 cgacggaccg gccgtcggcg tcagtggcca cgacccgggg gatgtcctcg tcccacagca
      481 tcgagaagtg gggatcgacc agcttgtcct caccgggcag gttcagccag atctggaaca
      541 gctcggtcgg gttgtcggcc tcctcctcca cgagggggaa catctcggcg tggacgatgc
      601 cgccgccggc ggtcagccac tggacgtcac cccggccgaa gcgggcggtg gcgccgaggg
      661 agtcggagtg gtccacgtag ccccggcgca cataggtcac ggtctcgaag cctcggtggg
      721 ggtgctgggg gaagccgggg acgaccaggc cgtggtacat gctccagccg tcccggcccg
      781 agaagtccat gccgaggtcc cgaccgtcga gtgaggcctg cggggccatg cgctcgtcgc
      841 cggggggata ggcgtcgcgg tggtggacgc agaagaggaa cgggtcgatg gtcggccacg
      901 gcgcggcgcc gagctccacg gtttggatga cgggatcgct cacgacgggg tcaaccgacc
      961 cgcgaccccg gagattcctg ccccggttcg actacccggc gcgcgcaggt cgtgacggag
     1021 ggtccccgaa tccgcgattc cggacaatta cgttctcgct cagcgtttct tacgcacgtc
     1081 cctccgagga ggtggtgacg atgtcatgga tcaccggttc agaaggggac tcgatccact
     1141 cgggttcctc ggcggtcact ggcccttccg gttgctgtca tgcggttgac cccgatgacg
     1201 gggccacggc gtgcggcacg atcatccgca gcctccagct gtgggatcag gtgccctgga
     1261 tccgggcccg gatggcggga ggcgagctct gccccgcctg catggccgtc accgaggagc
     1321 accaggtccc cgcctgagcc cctcgccccg cgccggcctg ctcagcgctg ggccaggacc
     1381 agggggaaca cggcgtcggt cccctgctcg cgtagcgcag ccccgaccac cgtcaccgtc
     1441 cagcgggagc tggccgtgtc gtccaccagc aggaccggac cgggcggcgc gtcgccgtcc
     1501 acggcgaagg ccccgaacac gttgaggacc tgttgggcgc tgttctcacg cgtggcctgc
     1561 gccgctttcg cgcgggcggt ggttacggcg gccacgaacg gcagcccgac ggcgtcggcc
     1621 agggcctcgg cgaaccccgc gacctcgggg cgatcgtccg agggcaccgc ggtgacccag
     1681 gtcggcgccg gcgtcggcgc ccagctgtcg accaggcggg cggcggcgcc caccagctcg
     1741 tcggcgtagg cgccggtctt gcgcccggcc cggacgcgag ttccccagcc gccgtcggtc
     1801 cacaggcaca gggcccggcc ctcctcgacc tgcagctcgg ccgggatctt gccccgcacc
     1861 tcgtccaggc cggacggcca ctggcgacgg gggtcgacga gcaggggctg gccgcggacg
     1921 aactcgatgg cccgggccac ctccgcgggg tcgaggggct cctcggccac cggggcgcag
     1981 ttgccgcagc gaccacacgg gccggaagcg gggtcgtcca actcggccag gaggagctgc
     2041 atgaggcagt ccgtggtgct cccgaagtcg tccatggcgg cctgctcgcg gcggcgctcg
     2101 gcggtgacgc gctcgacccg gtcggcgtcg taggcccagt cccgccccgt gcgggaccac
     2161 ttgccccctt ggcgggccac cgcgccctc
//
LOCUS       DASTFV010000467         3043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1730754, whole genome shotgun sequence.
ACCESSION   DASTFV010000467 DASTFV010000000
VERSION     DASTFV010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730754"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFP02_08610"
     CDS             complement(<1..294)
                     /locus_tag="VFP02_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HET9543128.1"
                     /translation="MSEISMGRRVGILGGTFDPPHIGHLGMAVEVRDALALDVVLLVV
                     AHEPWQKVGTRRVSPSADRLALVEAAVADLEGLEASDVEIRHGGPSYTADTLAT"
     gene            615..1445
                     /locus_tag="VFP02_08615"
     CDS             615..1445
                     /locus_tag="VFP02_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9543129.1"
                     /translation="MSDSVGQYLNEIGTVPLLNAQEERELAQIIEAGFEARKRLENGE
                     KGREIRKAIRAADAAKDRFIRANLRLVVSVARRYPLPPSMELLDLIQEGNLGLEHAVD
                     KFDWRKGFKFSTYATFWIRQAIGRALDQKASLVRLPGDRSASLRAALRQASGDGDELD
                     EEHARLHRLTTPTSLDRTIGDDDSNELVDLIPDWHAGPEQEVMDRAETQMVTDLLDVL
                     DARARYAVEQRFGLHDGRKRSYREVGEELGVTAEAARRLVKRAVNTVRDHASATIDAA
                     "
     gene            1503..2852
                     /locus_tag="VFP02_08620"
     CDS             1503..2852
                     /locus_tag="VFP02_08620"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HET9543130.1"
                     /translation="MTSAVPATEWTPSSWRERPAAQQPSWPDEAALEAALKTLAAMPP
                     LVFAGEARRLTESLGQVAAGKAFLVQAGDCAESFEAFSADSIRDKLKVILQMAVVLTY
                     STGVPVVKVGRIAGQFAKPRSADTETLDAVELPSFRGDLVNGIGFTEAARTADPERLL
                     TAYHQSASTLNLLRAFTKGGFADLSSVHGWNLEFLAASPEGRRYERLAGEIDRALRFM
                     AACGINTVSEPQLHEVDFSTSHEALVLGYEEALTRQDSLTGDWYDCSAHMLWVGERTR
                     QLDGAHVHFLSGVGNPVGSKLGPDATPDDAVALCEALNPDRVPGRLTLITRMGADHVA
                     ERLPPVLRAVQEAGHPVVWACDPMHGNTYTSGERKTRHFDDICREIEGFFSAHRSVGT
                     WPGGIHLELTGEDVTECLGGGDHVSELDRRYETMCDPRLNAGQSLDLAFRVSEFLET"
BASE COUNT          485 a         1133 c          968 g          457 t
ORIGIN      
        1 cgtcgccagg gtgtcggcgg tgtaggacgg cccgccgtgg cggatctcga catcggaggc
       61 ctcgaggccc tcgaggtcgg cgaccgcggc ctcgacgagg gccagccggt cggccgacgg
      121 gctcacccgg cgggtcccga ccttctgcca cggctcgtgg gccacgacaa gcaggaccac
      181 gtccaaggcg agcgcgtcgc ggacctccac ggccatgccg aggtggccga tgtggggtgg
      241 atcgaaggtc ccaccgagga tgccgacacg ccgacccatg gaaatctcac tcaccgtgct
      301 tgaggtcgac gctcagcgcg ccgaaagagg gaatccaccc gcaatgaatg gacttctgtc
      361 tgcctgtgcg cgatcctaga gagcgaaccg gctcccccct cgccccgtcg gctcactcgg
      421 cctgtcgtgc cggtgctctc cagtaccgat cgtccgtgtc gtcctgttct caccgtgctg
      481 tgaccgatgt catggtaccg gtcggaacgg ggtacgagtc ggggtcgttg ttcacattcc
      541 cccgccgtcg agaaagtcgc cgggtgcacg actgtcggaa tgacagcctt gtcccttgcg
      601 ttacgagaga tgccatgagt gattccgtag gccagtacct gaacgagatc ggcaccgtgc
      661 cgctcctcaa cgcccaggaa gagcgcgagc tcgcccagat catcgaggcc ggcttcgagg
      721 cccgcaagcg cctcgagaac ggcgagaagg gccgggagat ccgcaaggcc atccgggccg
      781 ccgacgccgc caaggaccgg ttcatccggg ccaacctccg cctggtcgtg agcgtggccc
      841 gccgctaccc cctccccccg agcatggagc tcctggacct catccaggag ggcaacctcg
      901 gactcgagca cgccgtcgac aagttcgact ggcgcaaggg gttcaagttc tccacctacg
      961 ccaccttctg gatccgccag gccatcggcc gggccctcga ccagaaggcc agcctggtcc
     1021 gcctccccgg cgaccgctcg gccagcctcc gggctgccct gcgccaggcc tcgggcgacg
     1081 gtgacgagct ggacgaggag cacgcccgcc tccaccgcct cacgaccccc acgtcgctgg
     1141 accgcaccat cggtgacgac gacagcaacg agctggtcga cctcatcccg gactggcatg
     1201 ccggtccgga gcaggaggtc atggaccggg ccgagaccca gatggtcacc gacctgctgg
     1261 acgtcctcga cgcccgggcc cgctacgccg tcgagcagcg cttcggcctc cacgacgggc
     1321 gcaagcgcag ctaccgggaa gtgggcgagg agctcggtgt gaccgccgag gccgcccgtc
     1381 gcctggtcaa gcgagccgtg aacacggtcc gcgatcacgc gtcggccacc atcgacgccg
     1441 cctgatccgt cccggtcggt tatcgcgttg cgatcgccga ccgggtccga cagcctcttg
     1501 tcgtgacctc tgccgtgcct gccaccgagt ggaccccgtc ctcctggcgg gagcgacccg
     1561 cggcccaaca gccgtcctgg cccgacgagg ccgccctgga ggccgccctc aagaccctgg
     1621 ccgccatgcc tccgctggtc ttcgccggtg aggcccgccg cctgaccgag agcctcggcc
     1681 aggtggccgc cggcaaggcg ttcctggtcc aggccggcga ctgcgccgag tccttcgagg
     1741 ccttctcggc cgacagcatc cgggacaagc tcaaggtcat cctgcagatg gccgtcgtgc
     1801 tgacctactc caccggtgtc cccgtcgtga aggtcggacg catcgccggc cagttcgcca
     1861 agccccgctc ggccgacacc gagaccctcg acgccgtcga actgccctcg ttccggggtg
     1921 acctggtcaa cggcatcggg ttcaccgagg cggcccgcac cgccgacccc gagcgcctgc
     1981 tgaccgccta ccaccagagc gcctccacct tgaacctgct gcgggccttc acgaaggggg
     2041 gcttcgccga tctgtccagc gtgcacggct ggaacctcga gttcctggcg gcgagccctg
     2101 agggtcgccg ctacgagagg ctcgcgggcg agatcgaccg tgccctccga ttcatggcgg
     2161 cctgcggaat caacaccgtc tcggagcctc agctccacga ggtcgacttc tcgaccagtc
     2221 acgaggccct cgtcctgggc tacgaggagg cgctgacccg tcaggactcc ttgaccggtg
     2281 actggtacga ctgctcggcc cacatgctct gggtcggcga gcgcacccgc cagctcgacg
     2341 gagcccacgt ccacttcctg tccggcgtgg gcaacccggt gggcagcaag ctcggcccgg
     2401 atgccacccc ggacgatgcc gtcgccctct gcgaggccct caaccccgac cgggtccccg
     2461 ggcgcctcac cctgatcacg cgcatggggg ccgaccatgt ggccgagcgg ctgcccccgg
     2521 tcctgcgggc cgtgcaggag gccgggcatc ccgtggtgtg ggcctgtgat ccgatgcacg
     2581 gcaacaccta caccagcggc gagcgcaaga cccggcactt cgacgacatc tgtcgcgaga
     2641 tcgagggctt cttctccgcc caccgctcgg tcggcacctg gcccggtggc atccacctgg
     2701 agctgaccgg ggaagacgtc accgagtgcc tcggcggcgg cgaccacgtg tccgagctcg
     2761 accgtcgcta cgaaaccatg tgcgaccctc gcctcaacgc cggccagtcc ctggacctgg
     2821 ccttccgggt ctcggagttc ctggagacct gaccccggtc ccgggcctcg aaggccgtga
     2881 ccgattccac cgagtaaatc ttgaccaaag tgatcagatt tgtcaggata atcgggaatc
     2941 ctgagtggac cactcgggtt tgaacgctct gagcgcaccc accccgctgc ccgaaagtcc
     3001 cccatgccca gcatcaccat cgatccggcg atccaggccg aca
//
LOCUS       DASTFV010000468         3767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1741237, whole genome shotgun sequence.
ACCESSION   DASTFV010000468 DASTFV010000000
VERSION     DASTFV010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741237"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFP02_08625"
     CDS             <1..240
                     /locus_tag="VFP02_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543131.1"
                     /translation="TNVAAAATIALVGPGRFSLDRVLRVRPSKLLIALAAWAGVGASV
                     GVIQGQVVRRAEQRSRPAPAANRVEPEYVDLLRSA"
     gene            336..938
                     /locus_tag="VFP02_08630"
     CDS             336..938
                     /locus_tag="VFP02_08630"
                     /inference="COORDINATES: protein motif:HMM:NF036807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9543132.1"
                     /translation="MTIRVVGAGLGRTGTTSLKAALEQLLGGPCHHMFEVAFNPEQVP
                     MWTAAARGEPVDWDEVLGGYRSIVDWPGVSFWRELTDHYPDALVLLSVRDPDAWYESA
                     RSTIFVSMLEDAKPDDPWTTFIRTLFGNRFTLDLDDKAATLAAFEAHYTAVRATVPAD
                     RLLEWTVADGWGPLCAALDVPVPDDPFPRLNTRDDWAPKD"
     gene            complement(942..>3767)
                     /locus_tag="VFP02_08635"
     CDS             complement(942..>3767)
                     /locus_tag="VFP02_08635"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HET9543133.1"
                     /translation="WRTHMALAPEQTRIGIRRHFTTEGTHPYDMVDWERRDARITNFR
                     DGTVAFEQTDVEFPTTWSLNATNIVAQKYFRGTLGTAEREWSLRQVIDRVADTITAWG
                     ARDGYFVDTDEAETFNAELKYLLVNQKVAFNSPVWFNIGVQGVPQQASACFILAVDDK
                     MDSILNWYVEEGTIFKGGSGAGINLSRIRSSQELLKGGGTASGPVSFMRGADASAGTI
                     KSGGKTRRAAKMVILDVDHPDVEEFIWCKAREEQKARVLRDAGFDMDLDGVDSHSTQY
                     QNANNSVRVTDEFMQAVVDDADWHLRAVTDGTILKTVRAKDLMRQISQATWECADPGM
                     QFDTTINHWHTAANTGRINGSNPCSEYMHLDNSACNLASLNLLTFLPENTDFDVEGFK
                     AAVEVIFTAQEILVGNADYPTGPIGENARRFRQLGLGYANLGALLMALGLPYDSDEGR
                     ALAGAITGLMTGHAYATSAVTAARMGPFAGYAENEEHMLRVLDQHREKMADIDEELVP
                     IDLLSAAQQAWDQACATAAIHGVRNAQASVLAPTGTIGLMMDCDTTGVEPDLGLTKMK
                     KLVGGGTMTIVNQTVPRALRNLGYSDGEVTAIVDYIDREKSILGAPALKAEHIPVFAC
                     SMGDNAIHYSGHVRMMGAVQPFISGAISKTVNMPEDVTVEDVEQLHIDAWRLGVKAVA
                     IYRDNCKVAQPLAMAKKEGASDDAPADAAAAPQVVERVVERVVQEPYRQKLTKTRRAR
                     TFEFRVADCKGFVTVGEYEDGRPGEIFISVSKQGSTLAGIMDAFAISISHGLQYGVPL
                     RSFVDTFTGMRFEPAGMTDDEDIRFATSLMDYLFRKLAILYLSPEERAELGIHTISER
                     TQPTLPGVEETVTETVQGQDITPDPPSIESASTLDLDLDLDLTQAKSPVPVADVNAPL
                     CMQCGIQMQRAGSCHACPSCGTTSGCS"
BASE COUNT          510 a         1253 c         1362 g          642 t
ORIGIN      
        1 acgaacgtcg cggcggcggc gaccatcgcc ctggtcggcc cgggacggtt cagcctcgat
       61 cgtgtgctgc gggtccgccc gtcgaagctg ctgatcgcgc tggcggcttg ggccggcgtc
      121 ggcgcgagcg tcggcgtcat ccagggtcag gtcgtccgcc gcgccgagca gcggtcgcgt
      181 ccggcgcccg ccgccaaccg ggtcgagccc gagtacgtcg acctcctccg ctccgcctga
      241 tcgaaccccg acccgccaca cccgcttctg gcctctgatc tgctccccct gacgagcgga
      301 tcagaggcca gaacggtgcg gtacggtcgc cggccatgac catccgggtg gtgggggcgg
      361 gactggggcg gacggggacg acctcgctga aggcggcgct cgagcagcta ctgggcgggc
      421 cgtgccatca catgttcgaa gtggccttca accccgagca ggtgccgatg tggaccgccg
      481 ccgccagggg ggagcccgtc gactgggacg aggtgctcgg tggctaccgg tcgatcgtgg
      541 actggcccgg cgtgtccttc tggcgggagc tgaccgacca ctaccccgac gccctcgtcc
      601 tgctgtcggt ccgggatccc gacgcctggt acgagagcgc ccgctcgacg atcttcgtgt
      661 cgatgctcga ggacgccaag ccggatgacc cgtggaccac gttcatccgc accctcttcg
      721 gcaaccggtt caccctcgac ctggacgaca aggccgccac cctggccgcc ttcgaggccc
      781 actacaccgc cgttcgcgcc accgtgcccg ccgaccggct cctcgagtgg acggtcgccg
      841 acggctgggg acccctgtgc gccgccctgg acgtccccgt ccccgacgac cccttccccc
      901 gcctcaacac ccgcgacgac tgggccccga aggactgagc gtcagctgca gccgctggtg
      961 gtgccgcagc tggggcaggc gtggcaggag ccggcgcgct gcatctggat gccgcactgc
     1021 atgcagaggg gggcgttgac gtcggccacg gggaccgggg acttggcctg ggtcagatcc
     1081 aggtccaggt ccaggtccag cgtcgacgcc gactcgatgg agggcgggtc cggtgtgatg
     1141 tcctggccct ggaccgtctc ggtcaccgtc tcctcgacgc cgggcagcgt gggctgggtc
     1201 cgctcgctga tggtgtggat gccgagctcg gcccgctcct cgggcgacag gtagaggatc
     1261 gccagcttgc ggaacaggta gtccatcagg ctggtggcga agcggatgtc ttcgtcatcg
     1321 gtcatgcccg cgggctcgaa gcgcatgccc gtgaacgtgt ccacgaagct gcgcagcggc
     1381 acgccgtact gcaggccgtg gctgatggag atggcgaagg cgtccatgat cccggccagg
     1441 gtcgagccct gcttggagac cgagatgaag atctcgcccg gacggccgtc ctcgtactcc
     1501 ccgacggtca cgaagccctt gcagtcggcc acccggaact cgaaggtgcg ggcccggcgg
     1561 gtcttggtca gcttctggcg gtacggctcc tggacgaccc gctcgacgac ccgctcgacg
     1621 acctgcggcg ccgccgcggc atcggccggg gcgtcgtcgg acgccccttc cttcttggcc
     1681 atggccaggg gctgggccac cttgcagttg tcccggtaga tggcgacggc cttcacgccg
     1741 aggcgccagg cgtcgatgtg gagctgctcg acgtcctcga cggtgacgtc ctcgggcatg
     1801 ttcaccgtct tggagatggc gccggagatg aagggctgga ccgcccccat catccgcacg
     1861 tgacccgagt agtggatggc gttgtcgccc atcgaacagg cgaacacggg gatgtgctcg
     1921 gccttcaggg ccggggcgcc gaggatcgac ttctcgcggt cgatgtagtc gacgatcgcg
     1981 gtgacctcgc cgtcggagta gccgaggttc cgcagggccc ggggcaccgt ctggttgacg
     2041 atggtcatcg tcccgccgcc gaccagcttc ttcatcttgg tcaggccgag gtcgggctcg
     2101 acgccggtgg tgtcgcagtc catcatcagc ccgatcgtcc ctgtgggggc gaggaccgag
     2161 gcctgggcgt tgcggacgcc gtggatggcg gccgtggcac aggcctggtc ccaggcctgc
     2221 tgggcggcgg acaggaggtc gatgggcacg agctcctcgt cgatgtcggc catcttctcg
     2281 cggtgctggt ccagcacccg cagcatgtgc tcctcgttct cggcgtagcc ggcgaaaggc
     2341 cccatgcggg cggcggtgac cgccgaggtg gcgtaggcgt ggccggtcat gagcccggtg
     2401 atggccccgg ccagggcccg gccctcgtcg gagtcgtagg gcaggcccag ggccatgagc
     2461 agggcgccga ggttggcgta gcccaggccg agctgacgga agcggcgggc gttctcgccg
     2521 atgggcccgg tcgggtagtc ggcgttgccg accaggatct cctgggcggt gaagatgacc
     2581 tcgacggcgg ccttgaagcc ttcgacgtcg aagtcggtgt tctcgggcag gaaggtcagc
     2641 aggttgaggc tggccaggtt gcaggccgag ttgtccaggt gcatgtactc cgagcagggg
     2701 ttcgacccgt tgatccgccc ggtgttggcc gcggtgtgcc agtggttgat ggtggtgtcg
     2761 aactgcatgc cgggatcggc gcactcccag gtggcctggg agatctggcg catgaggtcc
     2821 ttggcccgga ccgtcttgag gatggtgccg tcggtgaccg cccgcaggtg ccagtcggcg
     2881 tcgtcgacga cggcctgcat gaactcgtcg gtgacccgga ccgagttgtt ggcgttctgg
     2941 tactgcgtgg agtggctgtc gacgccgtcg aggtccatgt cgaagccggc gtcccgcagg
     3001 acccgggcct tctgctcctc ccgggccttg caccagatga actcctcgac gtcggggtgg
     3061 tccacgtcga ggatgaccat cttggcggcc cggcgggtct tgcccccgga cttgatggtg
     3121 ccggcggagg cgtcggcgcc ccgcatgaag ctgaccgggc cactggcggt gcccccaccc
     3181 ttcagcagct cctggctgga gcggatgcgg gacaggttga tgcccgcccc cgagccgccc
     3241 ttgaagatcg tgccctcctc gacgtaccag ttgaggatgg agtccatctt gtcgtcgacg
     3301 gcgaggatga agcaggcgct ggcctgctgg ggcacgccct gcaccccgat gttgaaccag
     3361 accgggctgt tgaaggccac cttctggttc accagcaggt acttgagctc ggcgttgaag
     3421 gtctcggcct cgtccgtgtc gacgaagtag ccgtcccggg cgccccacgc ggtgatggtg
     3481 tcggccaccc ggtcgatgac ctggcgcaac gaccactcgc gctcggcggt gcccagcgtc
     3541 ccccggaagt acttctgggc cacgatgttc gtggcgttca gcgaccaggt ggtcgggaac
     3601 tccacgtcgg tctgctcgaa ggcgaccgtg ccgtcccgga agttggtgat ccgcgcgtct
     3661 cgccgttccc agtcgaccat gtcgtagggg tgggtcccct cggtcgtgaa gtggcggcgg
     3721 atgccgatgc gggtctgctc gggggcaaga gccatgtggg tcctcca
//
LOCUS       DASTFV010000469         2802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1750514, whole genome shotgun sequence.
ACCESSION   DASTFV010000469 DASTFV010000000
VERSION     DASTFV010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750514"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /gene="prmC"
                     /locus_tag="VFP02_08640"
     CDS             complement(<1..1212)
                     /gene="prmC"
                     /locus_tag="VFP02_08640"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET9543134.1"
                     /translation="MTWASLRREVEAQLREAGHEAAAQEARWIVETASGHEGAEHLAV
                     ADQAPPPRGVVAVDQMVERRLAGEPIQYVLGSWAFRTLDLFVDGRVLIPRPETEIVTG
                     HALAEADRRRAEGAAPVLVADLGTGSGAIGLAIAAERDWTEVWLTDASADALDVARAN
                     LAGLGRPAARVTVAEGTWFAALPDDARGSVDVIVSNPPYIGADEGLLPSVADWEPESA
                     LVAGPAGTEDLEHLIDEARTWLAPGGALVLELAPHQAEPMAARLRGQGYTAVRIEADL
                     AGLDRVLVGRVPDPGPSAAIVALRAGGAVVVPTDTVYGLAVRADLPGAVDRLAALKDR
                     AAEQPVAVLVASIEQADAVGVLSVDARRLAERWWPGPLTLVLPRRPGLDWDLGEPADT
                     VGVRVPDHDVVR"
     gene            complement(1220..2284)
                     /gene="prfA"
                     /locus_tag="VFP02_08645"
     CDS             complement(1220..2284)
                     /gene="prfA"
                     /locus_tag="VFP02_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET9543135.1"
                     /translation="MLERLEDFEREFDDVEAHLADPDVFADQERYVALTRRHKELDAI
                     VSTGRALRQRQEDLSTAREMLTDAAGDDRETVRAEVDDAEAAIAELSEQYRLLLLPKD
                     PNDDKNVIVEVRGAEGGEEANLFARDLFDMYRAYAGRMGWAFEVLGSQPSDMGGVSEA
                     SFLLKGDGVWSRLKHEGGTHRVQRVPVTESQGRIHTSSATVTVLPEAEEIDVELDPND
                     LQVDVYRSSGPGGQSVNTTDSAVRITHKPTGLVVAMQDEKSQLQNRARALVVLRSRLL
                     KAEQDAAAAEASSARRSQVGAGGRADKIRTYNVKENRLTDHRIGLTLYKLDKVLAGEL
                     DEVVDALVADERARQLQGEY"
     gene            complement(2289..>2802)
                     /locus_tag="VFP02_08650"
     CDS             complement(2289..>2802)
                     /locus_tag="VFP02_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543136.1"
                     /translation="ALEVGVGRHDREAFAMVHGDLPTREALASVIATVRENRRVGAEP
                     HPLSRLAQERWVRAVLVERPDLVGCARLAPVVPLLARDSVKDVVPACLLGEDLEGRTV
                     VVACSVGIDLDLVPTAAEVRAHYAPDARLLLALPARDDSPVTRRIAARLRDPAEIVVL
                     PDDWRSAALA"
BASE COUNT          355 a         1038 c         1033 g          376 t
ORIGIN      
        1 gcgcacgacg tcgtggtcgg ggacccggac cccgacggtg tccgccggct cgccgaggtc
       61 ccagtcgagc ccggggcgtc ggggcaggac cagcgtgagc gggcccggcc accaacgctc
      121 ggccaggcgg cgggcgtcca cggagagcac tccgacggcg tcggcctgtt cgatcgaggc
      181 cacgagcacg gcgacgggtt gctcggcggc ccgatccttc agcgcggcca gccggtcgac
      241 ggcgcccggg aggtcggccc ggaccgccag cccgtacacc gtgtcggtcg gcaccaccac
      301 tgcgccgccg gcccgcaacg caacgatcgc cgcactcggt cccgggtccg gaacgcgccc
      361 gacgaggacc cggtcgagac cggcgaggtc cgcctcgatg cggaccgccg tgtagccctg
      421 acccctcagg cgggccgcca tgggctcggc ctggtgggga gccagctcga gcacgagcgc
      481 cccgccggga gccagccacg tccgggcctc gtcgatgagg tgctcgaggt cttcggtgcc
      541 ggccgggccg gccaccagcg ccgattccgg ctcccagtcg gccaccgacg gcaggaggcc
      601 ctcgtcggcc ccgatgtacg gcgggttgct gacgatcacg tcgacgctcc cccgggcgtc
      661 atcgggcagt gccgcgaacc aggtgccttc agccacggtc acccgggccg ccgggcgacc
      721 cagcccggcc aggttcgccc gggccacgtc cagagcatcc gccgatgcgt cggtcaacca
      781 gacctcggtc cagtcccgct cggcggcgat cgccaggccg atggcccccg aacccgtgcc
      841 caggtcggcg acgaggaccg gggccgcgcc ctcggcgcgg cgccggtccg cctcggccag
      901 ggcgtggccg gtgacgatct ccgtctccgg ccgcgggatg aggacccggc cgtcgacgaa
      961 cagatcgagg gtgcggaagg cccagcttcc gaggacgtac tggatcggct ccccggccag
     1021 ccggcgctcg accatctggt cgacggcgac cacgccccgc ggcggggggg cctgatcggc
     1081 gacagccagg tgctcggccc cctcgtgacc cgaggccgtc tcgacgatcc accgggcctc
     1141 ctgggccgcg gcctcgtggc cggcctcgcg tagctgggcc tcgacctcgc gccgcagcga
     1201 ggcccaggtc atggcctggt cagtactcac cctggagctg ccgggcccgt tcgtcggcga
     1261 cgagggcgtc gaccacctcg tccagctcgc cggccaggac cttgtccagc ttgtagagcg
     1321 tgaggccgat gcggtggtcg gtgagccggt tctccttgac gttgtaggtc cggatcttgt
     1381 cggcccgacc gccggccccg acctggctgc ggcgggcgga ggaagcctcg gcggcggccg
     1441 cgtcctgctc ggccttcagg agccgcgagc gcaggacgac gagggcccgg gcccggttct
     1501 ggagctggct cttctcgtcc tgcatggcca cgaccaggcc ggtcggcttg tgggtgatgc
     1561 gcacggcgga atcggtggtg ttgaccgact gcccaccggg gcccgatgag cggtacacgt
     1621 cgacctgcag gtcgttgggg tcgagctcga cgtcgatctc ctcggcctcg ggcaggacgg
     1681 tgaccgtcgc cgacgacgtg tggatgcgcc cctgggactc ggtgacgggc acccgctgca
     1741 cgcgatgcgt gcctccctcg tgcttgagcc ggctccagac gccgtccccc ttcagcagga
     1801 acgaggcttc gctcaccccg cccatgtccg acggctggct gccgagcacc tcgaaggccc
     1861 agcccatgcg cccggcatac gcccggtaca tgtcgaagag gtcccgggcg aagaggttgg
     1921 cctcctcgcc cccctccgca ccgcggacct cgacgatgac gttcttgtcg tcgttggggt
     1981 ccttgggcaa caggagcagg cggtactgct cggacagctc ggcgatggcg gcctccgcgt
     2041 cgtcgacctc ggcccgcacc gtctcccggt cgtcgcccgc cgcgtcggtc agcatctccc
     2101 gggccgtgga caggtcttcc tggcgctgcc gcagggctcg cccggtgctg acgatggcgt
     2161 ccagttcctt gtgtcgtctg gtcagcgcga cgtagcgctc ctggtcggcg aagacgtcgg
     2221 gatcggcgag gtgggcctcg acgtcgtcga actcgcgctc gaagtcctcg agccgctcca
     2281 gcatgggatc aagctagggc ggcggagcgc cagtcgtccg ggaggacgac gatctcggcg
     2341 gggtctcgga ggcgggcggc gatgcgccgg gtcacggggc tgtcgtcgcg cgccgggagg
     2401 gccaacagca ggcgagcgtc cggcgcgtag tgggcccgta cctcggccgc ggtcggcacc
     2461 aggtccaggt cgatgcccac cgagcaggcg accacgacgg tgcgcccctc gaggtcctcc
     2521 ccgagcaggc aggcgggcac gacgtccttg accgagtcgc gggccagcag gggcacgacc
     2581 ggcgccagcc gggcacagcc caccaggtcg ggccgctcga cgagcacggc gcggacccac
     2641 cgctcctgag ccagccggct gagcgggtgg ggctcggcgc cgacgcggcg gttctcccgc
     2701 acggtggcga tgaccgaggc gagggcctcc cgcgtgggca ggtcgccgtg gaccatggcg
     2761 aaggcctctc ggtcgtggcg gcccacgccg acctccaggg cc
//
LOCUS       DASTFV010000470         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1752673, whole genome shotgun sequence.
ACCESSION   DASTFV010000470 DASTFV010000000
VERSION     DASTFV010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752673"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..867)
                     /locus_tag="VFP02_08655"
     CDS             complement(235..867)
                     /locus_tag="VFP02_08655"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543137.1"
                     /translation="MGRLEGHPADLHGRALPTLIARRIDVLVPMTSAVVLGSTQAMPE
                     NEAIGLDVVRRRSGGGAVLVTPDDPVWVDVDLPAGDPRWSDDVGRSFLWLGEAWAAAL
                     AALGLDGKVHRGPFEPGRWGRAVCFAGRGPGEVFVEGAKVVGLAQRRTRAGARFQCAV
                     LRRWEPERLIEAVVATAERAAAQRDLPAVARGLDAEPEAVVSAFLSTLGT"
     gene            complement(884..1378)
                     /locus_tag="VFP02_08660"
     CDS             complement(884..1378)
                     /locus_tag="VFP02_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET9543138.1"
                     /translation="MSRALVLNASYEPLSVVSDRRAAVLVLTDKAEVVADTGAVLHAA
                     EIEVPVPSVVRLRYFVRVPFERRASLNRRAVFARDDHSCQYCGRPADSLDHIVPRSRG
                     GEHVWENVVAACRPCNVAKRDRMLEDSGMRLRRTPRAPRRLSWLLVAVGSIPEPWMPY
                     VDAA"
     gene            1511..2074
                     /locus_tag="VFP02_08665"
     CDS             1511..2074
                     /locus_tag="VFP02_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543139.1"
                     /translation="MAATQKKTGATARTSTAKKPAAKATSAAKKPAAAGKPAAKAAAK
                     PAAKKPAAKAPAKKPAAKKSPFTPAFIAKQKALLEEERAKFLRQAIDKQQEADQLVAD
                     RDPGDVQFDEESGEGDSIAVERSLDLALSAQAQDAVQQIDDALARIENGTYGISIISG
                     EPIPKARLEFIPWAAERVEEKVAKLGS"
BASE COUNT          361 a          827 c          766 g          260 t
ORIGIN      
        1 cccttgtcat cgagcttgcg cttcgacttg ccgacgaaca cgggccgttg cctcgggtgg
       61 tggtgtgctg catgggtcct ccgacgccat ggcccccctg tagggacgct tcaccactat
      121 cggtccctct acctccaaag tcaagccact tctactccaa atttactcca ctactcctcc
      181 caacgactcc cctgtggtcc acaaccgctc cgccgcggac ccgacctcga ccggtcaggt
      241 ccccaaggtg gacaagaaag ccgagacgac ggcctccggt tccgcgtcga ggccccgggc
      301 cacggccggc aggtcgcgct gggccgccgc ccgctcggcg gtggccacga cggcctcgat
      361 caaccgctct ggttcccagc ggcggagcac ggcgcactgg aagcgggcgc cggcccgggt
      421 gcggcgttgg gcgaggccga cgaccttggc cccctccacg aacacctcgc cggggccgcg
      481 gccggcgaag cacaccgccc tgccccaccg accgggctcg aacggtccgc ggtggacctt
      541 gccgtcgagg cccagggcgg ccagcgccgc ggcccaggcc tcgcccagcc agaggaagga
      601 acggccgacg tcgtcggacc agcgggggtc gccggcgggc aggtcgacgt cgacccacac
      661 cgggtcatcc ggcgtcacca gcaccgcgcc accgccggag cggcgccgca ccacgtccag
      721 cccgatcgcc tcgttctcgg gcatcgcctg ggtcgagccc agcacgaccg ccgatgtcat
      781 cggcaccagc acgtcgatgc gtcgggcgat caacgtaggc agcgcccgcc cgtggagatc
      841 ggccgggtgg ccctcgagtc gccccaccgc ccacggggcg gggtcaggcg gcgtcgacgt
      901 agggcatcca gggctcgggg atcgacccca cggcgaccag gagccagctc agccgccgtg
      961 gggcacgggg ggtccgccgc agccgcatgc ccgagtcctc gagcatgcgg tcccgcttgg
     1021 cgacgttgca gggccgacag gcggcgacca cgttctccca cacgtgctcg ccgccccgac
     1081 tgcgggggac gatgtggtcg aggctgtcgg cgggacggcc gcagtactgg cacgagtggt
     1141 cgtcccgggc gaagacggct cgccggttga gggaggcgcg acgctcgaag ggcacccgca
     1201 cgaagtagcg gaggcggacg accgagggca cgggcacctc gatctcggcg gcgtgcagga
     1261 cggcgcccgt gtccgccacc acctcggcct tgtcggtgag gaccaggacg gcggcgcgac
     1321 ggtccgagac gaccgagagc ggctcgtagc tcgcgttcag taccagggcc cgagacacgc
     1381 cccgacccta gttgtgcgtc cgaccccgat cggagcgttc tgaccgcctc tatggggccg
     1441 tggttgccgt cgctccgtgc cgccccctac cctgccccga ctttccgacc acgggggacg
     1501 gttgacacag atggcggcca cccagaagaa gaccggtgcc acggctcgca cgagcaccgc
     1561 gaagaagcct gccgcgaaag cgaccagcgc ggccaagaag ccggcggcag ccggcaagcc
     1621 tgcagccaag gccgcggcga agccggcggc caagaagccg gcggccaagg ccccggcgaa
     1681 gaagccggcg gccaagaagt cgccgttcac gccggcgttc atcgccaagc agaaggccct
     1741 cctcgaggag gagcgggcca agttcctgcg ccaggccatc gacaagcagc aggaggccga
     1801 ccagctggtg gccgaccggg acccgggtga cgtgcagttc gacgaggaat cgggcgaggg
     1861 cgacagcatc gccgtggagc gttccctcga cctggccctg tcggcccagg cccaggacgc
     1921 cgtccagcag atcgacgacg ccctggcccg catcgagaac ggcacctacg ggatcagcat
     1981 catctcgggc gagcccatcc ccaaggcccg tctggagttc atcccctggg cggcggagcg
     2041 ggtcgaggag aaggtcgcca aactcggcag ctgagccccc ggccagccct aggctggcgg
     2101 ctccccctga gcacagaggc cgcgagtgac cgacaacgcc gagacgtccg gtccgtaccc
     2161 ggacgtcacg cccaacgccg actaccccgc cgtcgagcgc ggcgtgctgg agcg
//
LOCUS       DASTFV010000471         3045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1756340, whole genome shotgun sequence.
ACCESSION   DASTFV010000471 DASTFV010000000
VERSION     DASTFV010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756340"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..912
                     /locus_tag="VFP02_08670"
     CDS             67..912
                     /locus_tag="VFP02_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9543140.1"
                     /translation="MTRWTVGAVRITKVPQMTWHLPLHGLVPEATADACARVAPGLID
                     DGVAEVSLHGLVIESGDRRILVDTCAGHDETAERILGVVRDFGSITDHHHDVQAAVGH
                     AGWAPEDIDTVVCTHLHFDHVGGNLADGEPAFPSARYVMVGDEWAFWSTNESDRQYAS
                     VDGSVRPLVDGGLADLVDTDHRLSDTVALVPTLGHTPGHVSVLIESGGQQAVITGDLA
                     HHPVELLAPEWKMVADEDPAQASTTRKAFVDRFGDGETLILGTHFGGSSAGYLDASTA
                     TWTRA"
     gene            938..2092
                     /locus_tag="VFP02_08675"
     CDS             938..2092
                     /locus_tag="VFP02_08675"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET9543141.1"
                     /translation="MSAVGAPPFAPVELGAFHAHEVQGLLTEHAEVLARRPDDLGPLT
                     LSVGEDAVTYRVAPGGVEVVEGVQAPRTHVALSPEAWSDFVHELRSAFGLGYAQLADC
                     CVGRIDGLCRWEATLRAAWVGRDPALPDVEPLGPDAPLADQLAMNGFALVPGVFTPDE
                     VETLRSEVTRLTALARNDDQRSWWARTAEAEEVCCRLLYTEEQSELITEVVTGDDRLA
                     DLLAPTGETVRLAADRFDGLSVVMKPPGVVEGLADLPWHRDCGLGGHSIMCPEFNVGI
                     QLEAASAETGHLEFLPGSHASGWWPLDPHSVDGRPIVEVDTQPGDVTVHYGHVLHRAG
                     SPTGEGGRRALYATFVQEHAFEYVGPHQAINDVLFASADGGVLDDRGTAG"
     gene            complement(2099..2923)
                     /locus_tag="VFP02_08680"
     CDS             complement(2099..2923)
                     /locus_tag="VFP02_08680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9543142.1"
                     /translation="MSPVALVTGASRGIGRATAFALAEKGFDVALGARTLHEGEGRDD
                     ARPGSAPLPGSLDATACAVEALGVRALPVRMDLHERESLTAGAATVLDDWGRIDVLVN
                     NAVDTGPGSMTTVADTTLDDYQRKLEGNFLAQLVLIQAVLPHLLERGEGTIVDITSAA
                     GITDPPAPAGEGGWGLAYAASKAAFHRVAAHLAVEFGPRGVLAFNVEPGMVMTEKMAI
                     NQKAMGLENRFPVAPPSVPAAVVAWLCTDSEAADHNGETFAAQKFAKERGLHPTWW"
BASE COUNT          413 a         1096 c         1113 g          423 t
ORIGIN      
        1 cgcccacgcc cgccccaaag gcggcttctt cggccaccac ctgcgcaagc actggatcga
       61 cgccccgtga ccaggtggac cgtcggcgcc gtccgcatca ccaaggtccc gcagatgacc
      121 tggcacctcc cgctccacgg cctggtgccg gaggccacgg cggacgcttg tgcccgggtg
      181 gcgccggggc tgatcgacga cggcgtggcc gaggtctccc tgcacggctt ggtcatcgag
      241 tcgggtgatc gacggatcct ggtcgacacc tgcgccggcc acgacgagac ggccgaacgg
      301 atcctgggtg tcgttcgcga cttcggctcg atcaccgatc accaccacga cgtccaggcg
      361 gcggtcggcc acgcggggtg ggcccccgag gacatcgaca cggtcgtctg cacccacctg
      421 cacttcgacc atgtgggcgg caacctggcc gacggcgagc ccgctttccc gtcggcccgc
      481 tacgtcatgg tcggcgacga gtgggccttc tggtcgacga acgaatctga caggcagtac
      541 gcatcggtcg acggcagcgt ccggccgctc gtcgacgggg gtctggccga cctggtcgac
      601 accgaccacc gactgagcga caccgtggcg ctggtgccca cgctcggtca caccccgggc
      661 cacgtcagcg tgctcatcga gtccggcggg cagcaggcgg tgatcaccgg tgacctcgcc
      721 catcacccgg tcgagctcct ggcccccgag tggaagatgg tcgccgacga ggacccggcg
      781 caggcctcga cgacccggaa ggcgttcgtc gaccgcttcg gcgacgggga gacgctgatc
      841 ctgggcaccc acttcggcgg ctcctccgcg ggctacctcg atgcctccac cgccacttgg
      901 acccgcgcat gaccccgccc accggggggt ggtcggtgtg agtgcagtcg gggcgccacc
      961 gttcgcgccc gtcgagctcg gggcgttcca cgcccacgag gtgcaagggt tgctgaccga
     1021 gcacgcggaa gtgctggccc ggcggcccga tgacctcggc ccgctcacgc tgtccgtcgg
     1081 cgaggacgcc gtcacctacc gggtcgcgcc gggcggggtg gaggtcgtcg aaggcgtgca
     1141 ggcgccccgg acccacgtgg ccctgtcacc cgaggcgtgg agcgacttcg tccacgagct
     1201 gcgctccgcc ttcgggctcg ggtacgcaca gctggccgac tgctgcgtcg gccggatcga
     1261 cggcctgtgc cgttgggagg ccacgctgcg cgccgcctgg gtcggaaggg acccggcgct
     1321 gccggacgtc gagccgctgg ggccggacgc gccgctggcg gaccagctgg ccatgaacgg
     1381 cttcgcgctg gtgcccgggg tcttcacgcc agacgaggtc gagacgctgc gctcggaggt
     1441 gacccggctc accgcgctgg ctcgcaacga tgaccagcgg tcgtggtggg cgcgcaccgc
     1501 cgaggccgag gaggtctgct gccgcctgct ctacaccgag gagcagtcgg agctgatcac
     1561 cgaggtcgtc acgggcgacg accgtttggc cgacctgctc gcaccgaccg gcgagaccgt
     1621 gcgcctggcc gccgaccgct tcgacggcct gagcgtggtc atgaagccgc ccggcgtcgt
     1681 ggaggggctg gccgacctgc cgtggcaccg ggactgcggc ctgggtggcc actcgatcat
     1741 gtgcccggag ttcaacgtcg gcatccagct ggaggccgcc agcgccgaga ccggccacct
     1801 ggagttcctc cccggatcgc acgcctccgg atggtggccg cttgaccccc actcggtcga
     1861 cggccgcccg atcgtggagg tcgacaccca gcccggcgac gtcacggtcc attacggcca
     1921 cgtcctgcac cgggccggct cgccgacggg ggagggcggg cgccgcgcgc tgtacgccac
     1981 cttcgtccag gagcacgctt tcgagtacgt cgggccccac caggccatca acgacgtcct
     2041 cttcgccagc gccgacggcg gcgtcctcga cgaccgcggc accgccggct gaggcgggtc
     2101 accaccaggt ggggtggagt ccgcgctcct tggcgaactt ctgcgcggcg aaggtctcgc
     2161 cgttgtggtc ggcggcttcg gagtcggtgc acagccaggc cacgacggcc gccgggaccg
     2221 agggcggggc gacggggaac ctgttctcca ggcccatggc cttctggttg atggccatct
     2281 tctcggtcat gaccatgccc ggctcgacgt tgaaggccag gacgccccgg gggccgaact
     2341 cgacggccaa gtgggcggcg acccggtgga aggccgcctt cgaggcggcg taggcgagtc
     2401 cccaaccccc ctcgccggcg ggcgcgggcg ggtcggtgat cccggccgcc gaggtgatgt
     2461 cgacgatcgt gccctcgccc cgctcgagga ggtgggggag cacggcctgg atgaggacga
     2521 gctgggcgag gaagttgccc tccagcttgc gctggtagtc gtccagggtc gtgtcggcga
     2581 cggtggtcat ggagccgggg ccggtgtcca cggcgttgtt gaccaggacg tcgatgcggc
     2641 cccagtcgtc gagcaccgtc gccgcgccgg cggtcagcga ctcccgctcg tggaggtcca
     2701 tgcgcacggg cagcgcccgg acgccgagcg cttcgaccgc gcacgccgtg gcgtcgaggc
     2761 tgccgggcag tggcgcggaa ccggggcggg cgtcgtcccg gccttccccc tcgtggaggg
     2821 tgcgggcgcc gagggcgacg tcgaagccct tctcggccaa cgcgaacgcc gtggcccgcc
     2881 cgatgccccg gctggccccg gtgacgagcg cgaccgggct caaccgccga ggccctcgaa
     2941 gtcgaccaag ccttcgtcca ggtccgtctt gaagccggcg tcggcgatga ggtcgatgcc
     3001 cgacacgtag gaagcgcccg gcgcgcacag gaagacgagg ggcag
//
LOCUS       DASTFV010000472         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1758095, whole genome shotgun sequence.
ACCESSION   DASTFV010000472 DASTFV010000000
VERSION     DASTFV010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758095"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="VFP02_08685"
     CDS             complement(<1..151)
                     /locus_tag="VFP02_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HET9543143.1"
                     /translation="MAPVLQLVVGDIIELDVDAVVNAANSSLLGGGGVDGAIHDAAGP
                     ELLAAC"
     gene            complement(183..716)
                     /locus_tag="VFP02_08690"
     CDS             complement(183..716)
                     /locus_tag="VFP02_08690"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HET9543144.1"
                     /translation="MAFVGAWATLGTRLPGYDPTQDAISRLAADGAPDQAWMTAGFVT
                     FGIGVPLFAVALRDALPGPAWTTAMATGLATLGVAALPLDGWAGDTAHGVAAGIGYVT
                     LAATPLLAAGPLARLGRRRAAVASRSLGIASLACLGATTLDDRAGLFQRLGLTLGDLW
                     LVAAAVGILRGGYGRPE"
     gene            955..1989
                     /locus_tag="VFP02_08695"
     CDS             955..1989
                     /locus_tag="VFP02_08695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET9543145.1"
                     /translation="MRRFRLLACLLLPLLLLAVACGDDSGDETLQADGEDAEAAGVPD
                     ATVGEPAEDDPSLDAITVGGTYAESATVEVDAPFSTETTYRRILEEGDGDPVEAGQSV
                     DFQNVAVNGRDGTEFDNSYENTGEGGGPPNIVMDETQIIPGLVRGLLDVPVGSRVLIA
                     IAPQDAFQAQGGIPDAGIEADDTIIFVVDVLDATTPEPPLARAEGTAVEPDEGLPTVE
                     LDDTGKPTITIPDGDPPTEQVTQVLIEGDGPEVESGQTITAHYTGVIWASGEQFDSSW
                     DRGEPSEFPIGVGGVIVGWDEGLVGQTVGSQMLLVIPPDKGYGPDGNPDAGIAGTDTL
                     VFVVDILAAN"
     gene            2065..>2283
                     /locus_tag="VFP02_08700"
     CDS             2065..>2283
                     /locus_tag="VFP02_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET9543146.1"
                     /translation="MPAVTVENPLVLPRVTADPEAHARPVDQVVPAHHAVEGAGFEVW
                     RPFPGNVDAHVADPFFLLDQLGPVDYAPN"
BASE COUNT          342 a          856 c          792 g          293 t
ORIGIN      
        1 gacaagcggc cagcagctcc ggtcccgcgg cgtcgtggat ggccccatcg acgccgcccc
       61 cgccgaggag cgacgagttc gcggcattca cgacggcgtc gacgtcgagc tcgatgatgt
      121 cgccgaccac gagctggagc acgggagcca cgagttgcct caggcgggat cggctcgatt
      181 ggtcactccg ggcgaccgta cccgcccctc aggatgccca ccgcggcggc gaccagccag
      241 aggtcgccga gggtgaggcc caggcgctgg aacaggccgg cgcggtcgtc cagcgtcgtg
      301 gccccgaggc acgccaggga ggcgatgccg aggctccggg acgccaccgc cgcccggcgg
      361 cggccgaggc gggccagggg accggcggcc aggaggggag tggctgcgag cgtcacgtac
      421 ccgatacccg cggccacccc gtgggcggtg tcgcccgccc agccgtcgag gggcagcgca
      481 gccacgccga gcgtggccag accggtggcc atcgccgtgg tccacgccgg cccggggagg
      541 gcgtcgcgca gggcgacggc gaacaggggc acgccgatgc cgaaggtcac gaacccggcc
      601 gtcatccagg cctggtccgg cgccccgtcg gcggccagcc ggctgatggc gtcctgggtc
      661 gggtcgtagc cggggagccg cgtgccgagc gtggcccacg cccccacgaa cgccaccggc
      721 ccgatcaccc ctccgagcgc gagtcgtctg gtcgtccccg ggatgtttcc agctctccga
      781 ggacgcacaa agtcccctcc cgccgggcca ctcccgggcg cccacccgct ggctcgtggc
      841 tcacgacgtg ctggcctcct tggcggctga cggtggtttc gtagccctga acgctgactg
      901 acggctcggg ctggcatcct gacgacccgg atcccaccga tcgaaagagt tcccgtgcgc
      961 cgattccgcc tgctcgcctg tctcctgctg ccgttgttgc tgctcgcagt ggcctgtggc
     1021 gacgactccg gcgacgagac gctccaggcc gacggcgagg acgccgaagc cgcgggcgtc
     1081 cctgacgcca cggtgggcga gcccgccgag gacgatcctt cgctcgacgc cataacggtc
     1141 ggcggcacct acgccgagtc cgccacggtc gaggtcgacg cccccttcag caccgagacg
     1201 acctaccgcc gcatcctcga ggagggcgac ggtgacccgg tcgaggccgg ccagtccgtc
     1261 gacttccaga acgtggccgt caacggccgg gacggcaccg agttcgacaa ctcctatgag
     1321 aacaccggcg agggtggcgg gccgcccaac atcgtcatgg acgagaccca gatcatcccc
     1381 ggcctggtgc gggggttgct cgacgtaccc gtcggcagtc gggtgctgat cgccatagcc
     1441 ccccaggacg ccttccaggc gcagggcggc atccccgacg ccggcatcga ggccgacgac
     1501 acgatcatct tcgtggtcga cgtcctggac gccaccacac cggagccacc actggcgcgg
     1561 gccgagggca ccgccgtcga gccggacgag ggcctcccca cggtggagct ggatgacacc
     1621 ggcaagccca cgatcacgat ccccgacggc gatcccccga ccgagcaggt gacccaggtc
     1681 ctcatcgagg gcgacggccc cgaggtcgag tccggccaga ccatcacggc ccactacacc
     1741 ggcgtcatct gggccagcgg cgagcagttc gactcgtcct gggaccgggg cgagccgtcc
     1801 gagttcccga tcggcgtggg tggcgtcatc gtcgggtggg acgagggcct ggtcggccag
     1861 accgtcgggt cccagatgct gctcgtgatc ccgccggaca agggctacgg ccccgacggc
     1921 aaccccgacg ccggcatcgc cggcaccgac accctggtct tcgtcgtcga catcctggct
     1981 gcgaactagg tcatccgccg gcgggcatac gcacggccgg tcggtggttg atgcttgcgt
     2041 aagcaacaat ctgaggagcg tcgaatgccg gctgtcaccg tcgagaaccc cctggtcctg
     2101 ccgcgggtca ccgctgaccc cgaggcccac gcccgacccg tcgaccaggt cgtgcccgcc
     2161 caccacgcgg tggagggcgc cggcttcgag gtctggcgac cgttcccggg caacgtcgac
     2221 gcccacgtgg ccgacccctt cttcctgctc gaccagctcg ggccggtcga ctacgccccc
     2281 aac
//
LOCUS       DASTFV010000473         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1759213, whole genome shotgun sequence.
ACCESSION   DASTFV010000473 DASTFV010000000
VERSION     DASTFV010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759213"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="VFP02_08705"
     CDS             complement(<1..1285)
                     /locus_tag="VFP02_08705"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HET9543147.1"
                     /translation="MRRTPFNDGWTFRPKVDRFAELSGAGAEWEPVTLPHDAVIGTDR
                     SPDAGPATGYFPGGAWEYRRTLERGAEDAVTTMLEFEGVYRDAVVTVNGSVAGHRPSG
                     YSGFVVPLDHLLTVGENEVKVEATAHADSRWYSGGGIYRNVWLLEAGPVHLVPDHLEV
                     LTPEADDAGAVVTVTAAVKNRGAVRADAVVHAEVLDADGTVVASQASPVTVFPGATVT
                     ARQRLFVAGPRRWGPDDPHLYSCRATLREGDEVVDEDRTTFGIRTLALDPQQGLRING
                     ERVLLRGACVHHDNGPIGAATLDRAETRRVELLKDAGFNAIRSAHNPLSRSMLDACDR
                     LGVLVMDETFDIWAQTKSADDYSLRFDDWWEADVEAMVRKDVNHPSVVLYCIGNEIPD
                     GSSATGLQRSRALAEKVRSLDPTRFVTQAVTGMLVA"
     gene            1390..>2189
                     /locus_tag="VFP02_08710"
     CDS             1390..>2189
                     /locus_tag="VFP02_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET9543148.1"
                     /translation="MTTFDQIAPALERQAIELPSWAFGNSGTRFKVFAQPGVPRDPYE
                     KLADAAQVHAVTGLAPSVALHIPWDLVDDFDKLGRHAEDLGIRLGTINTNTFQDDDYM
                     LGSLCHVDERTRAKAVAHALECIEVMAVTGSRDLKVWLPDGLNYPGQADLRDRQDRLA
                     DSLAQIYAALSADQRLVLEYKFFEPAFYATDVPDWGTSLLHCLALGERAMVCLDTGHH
                     APGTNIEFIVMQLLRQGRLGAFDFNSRFYADDDLMVGAADPFQLFRIL"
BASE COUNT          328 a          784 c          731 g          346 t
ORIGIN      
        1 cggccacgag catcccggtc acagcctggg tcacgaaccg ggtgggatcg agggaccgca
       61 ccttctcggc cagggcccgg ctgcgctgaa gaccggtcgc cgacgacccg tcggggatct
      121 cgttgccgat gcagtacagg acgacactgg ggtggttgac gtccttgcgc accatcgcct
      181 cgacatcggc ctcccaccag tcgtcgaagc ggaggctgta gtcgtcggcc gacttcgtct
      241 gggcccagat gtcgaacgtc tcgtccatga ccagcacccc gagccggtcg caggcgtcga
      301 gcatcgaccg gctcaggggg ttgtgagcgc tgcggatggc gttgaaaccg gcgtccttca
      361 ggagctcgac ccggcgcgtt tcggcccggt caagagtggc cgcgccgatc gggccgttgt
      421 cgtggtggac gcaggccccg cgcagcagca cccgctcacc gttgatccgc agcccctgct
      481 ggggatcgag ggccagcgtc cggatcccga aggtcgtgcg gtcctcgtcc acgacctcgt
      541 cgccctcccg cagggtcgcc cggcaggagt agaggtgcgg gtcgtccggg ccccagcgtc
      601 gcgggccggc cacgaacagg cgttggcggg ccgtgaccgt cgcgccgggg aacactgtga
      661 cgggtgacgc ctggctggcc accaccgtcc cgtcggcatc gagcacctcg gcgtgcacaa
      721 cggcgtcggc cctcaccgca ccccggttct tgacggccgc ggtcaccgtc acgaccgcgc
      781 cggcgtcgtc ggcctcgggg gtgagcacct cgaggtggtc gggcaccagg tggaccggcc
      841 cggcctcgag gagccagacg ttccggtaga tgccgccacc cgagtaccaa cgcgagtcgg
      901 cgtgggccgt ggcctcgacc ttgacctcgt tctccccgac cgtgagcagg tgatcgagcg
      961 ggacgacgaa cccggagtag ccggacggcc ggtgaccggc gaccgacccg ttcacggtga
     1021 cgacggcgtc gcggtagacg ccctcgaatt ccagcatcgt cgtgacggcg tcctccgcac
     1081 cacgttccag ggtccggcgg tactcccagg cgccgccggg gaagtagccg gtcgccgggc
     1141 cggcatcggg cgagcgatcc gtgccgatga cggcgtcgtg gggcagggtg accggctccc
     1201 attcggcccc cgccccgctc agctcggcga accggtcgac cttcgggcga aaggtccagc
     1261 cgtcattgaa cggggtgcga cgcatggctc tccaccttac gccacctggt tgttagcgtt
     1321 atcatctcgt gctggacgca agtgttcgtt tgtgtgagac tgatcacgat cacacatcgg
     1381 gggagccgca tgacgacctt tgatcagatc gctccggcgc tggagcgtca ggccatcgag
     1441 ctgccgtcct gggcgttcgg gaactcgggg acccgcttca aggtgttcgc ccagccgggg
     1501 gtgccccggg acccctacga gaagctggcc gatgccgcgc aggtccacgc cgtgaccggg
     1561 ctggcgccat cggtcgccct ccacatcccc tgggacctgg tcgacgactt cgacaaactc
     1621 ggccgccacg ccgaggacct cgggatccgc ttggggacga tcaacaccaa cacgttccag
     1681 gacgacgact acatgctggg cagcctgtgc cacgttgacg agcggaccag ggccaaggcc
     1741 gtcgcccacg ccctcgagtg catcgaggtc atggcggtca ccggcagtcg ggacctgaag
     1801 gtctggctgc cggacgggct gaactacccg ggccaggccg acctgcggga ccgacaggac
     1861 cgcttggccg attcgctggc ccagatctac gccgcgctga gtgctgacca gcgcctcgtc
     1921 ctcgagtaca agttcttcga gcccgccttc tacgccaccg acgtccccga ctggggtacg
     1981 tcgctgctgc actgcctcgc cctcggcgag cgcgccatgg tctgcctcga caccggtcac
     2041 cacgctccgg gcacgaacat cgagttcatc gtcatgcagc tcctgcgcca gggccgcctc
     2101 ggcgccttcg acttcaactc ccgcttctac gccgacgacg acctcatggt cggcgccgcc
     2161 gacccgttcc agctgttccg catcctcta
//
LOCUS       DASTFV010000474         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1762280, whole genome shotgun sequence.
ACCESSION   DASTFV010000474 DASTFV010000000
VERSION     DASTFV010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762280"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VFP02_08715"
     CDS             complement(<1..617)
                     /locus_tag="VFP02_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HET9543149.1"
                     /translation="MAKRVVLAYSGGLDTSVAVRWLIDELGYEVVCLCADVGQEGSDW
                     GPVMERAKAAGAVEAEVVDVRGEFAKDFVVPALRANALYEGRYPLVSALSRPVIVRHL
                     VDAANRYGASAVAHGCTGKGNDQVRFEVGVRSIAPNLDVLAPVRTWGMTREDAILYAY
                     RHEIPITATKEKVYSIDDNLWGRAIECGEMEDPWAKPPPGVWSMT"
     gene            complement(628..1095)
                     /gene="argR"
                     /locus_tag="VFP02_08720"
     CDS             complement(628..1095)
                     /gene="argR"
                     /locus_tag="VFP02_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET9543150.1"
                     /translation="MSAKAQRQHKIARFLESEEVSSQAQLVELLAAEGVTATQATVSR
                     DLDDLGAIKVRARGGVTVYAVPELPKDQVPPEDHLRRVLGDWVVDVAHSGPLVVLRTP
                     PGSAHVVGSALDRSSLPEVLGTVAGDDTLIVVADEEVGGSSLADQLRELAGLS"
     gene            complement(1097..1987)
                     /gene="argF"
                     /locus_tag="VFP02_08725"
     CDS             complement(1097..1987)
                     /gene="argF"
                     /locus_tag="VFP02_08725"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET9543151.1"
                     /translation="MRHLLEIDDLTKDELFEVIALALDPNPPQVLSRKGMALVFQKPS
                     ARTRNSMEMAVKDLGGHPVTIRAEEVSIDQRESAEDVARTLACYHAAIGARVFDHSVL
                     ERMAKVAWVPIINLLSDDAHPLQAVADLLTMADEFGDLNGRTLAYVGDGNNMCRSLAL
                     AATLAGMQVRVASPPGYALSEVDVDRIAALGVAPELLDRPEEAVAGVDVVYTDVWTSM
                     GQETEVEARRKAFEGYMVDERLMALAAERSIFLHCLPAHRGEEVSHEVLDGDRSRVWR
                     QAENRMHAARGAVAWILGQV"
     gene            complement(1987..>2312)
                     /locus_tag="VFP02_08730"
     CDS             complement(1987..>2312)
                     /locus_tag="VFP02_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9543152.1"
                     /translation="QPLATAAARKVLEVMEREDVPARARDAGKRLADGLLDLPHVTGV
                     RGLGLLLAAELSDRDAREVATAALAAGLVVNGVSPTALRFAPSLLVDEAEIGEALSIL
                     EGILA"
BASE COUNT          325 a          826 c          831 g          330 t
ORIGIN      
        1 atggtcatcg accacacgcc cgggggcggc ttggcccacg ggtcctccat ctcgccgcac
       61 tcgatcgccc ggccccagag gttgtcgtcg atggagtaca ccttctcctt cgtcgccgtg
      121 atggggatct cgtggcggta ggcgtagagg atggcgtcct cccgggtcat gccccaggtg
      181 cggaccggag ccagcacgtc gaggttgggg gcgatggagc ggacgccgac ctcgaagcgg
      241 acctgatcgt tgcccttgcc cgtgcagccg tgggccacgg ccgacgcgcc gtaccggttg
      301 gcggcatcga ccaggtggcg gacgatgacg ggtcgggaca gggccgacac cagcgggtag
      361 cggccctcgt agagcgcatt ggcccgcagg gcggggacca cgaagtcctt ggcgaactcc
      421 ccccgcacgt cgacgacctc ggcctcgacg gcgccggcgg ccttcgcccg ctccatgacg
      481 gggccccagt cgctgccctc ctggccgacg tccgcgcaca ggcagacgac ctcgtagccc
      541 agctcgtcga tcagccaccg cacggcgacc gatgtgtcca ggccaccgct gtaggcgagc
      601 accactctct tggccatcac gttcctctca ggagaggcca gccagctcac ggagctgatc
      661 ggccaggctc gatccaccga cctcttcgtc ggccacgacg atgagcgtgt catctccggc
      721 gacggtgccc aggacctcgg gcaacgacga ccggtccagg gccgagccca ccacgtgggc
      781 cgagcccggc ggggtgcgca ggacgacgag gggcccgctg tgggcgacgt cgacgaccca
      841 gtcccccagg acccgacgca ggtggtcctc gggtgggacc tggtccttgg gcagctccgg
      901 cacggcgtag acggtgaccc caccccgggc ccggaccttg atggccccga ggtcatccag
      961 gtcgcgggac accgtggcct gggtggcggt cacgccctcg gcggccagca gctccacgag
     1021 ctgggcctgg ctggacacct cctcggactc gaggaagcgg gcgatcttgt gctgtcgctg
     1081 ggccttggcg ctcatctcag acctgcccga ggatccaggc cacagccccg cgggcggcgt
     1141 gcatgcggtt ctcggcctgg cgccagaccc ggctccggtc cccgtccagc acctcgtggg
     1201 agacctcctc gccgcggtgg gccggcaggc agtgcagaaa gatcgacctc tccgccgcga
     1261 gggccatgag ccgctcgtcg accatgtagc cctcgaaggc cttgcgccgg gcctcgacct
     1321 cggtctcctg gcccatcgac gtccacacgt cggtgtacac gacgtcgacg ccggccacgg
     1381 cctcctcggg gcggtcgagc agctcggggg cgaccccgag ggcggcgatc cggtcgacgt
     1441 cgacctcgga cagggcatag cccggggggc tggcgacccg gacctgcatg ccggccagcg
     1501 tggccgccag ggccagcgac cggcacatgt tgttgccgtc gcccacgtag gcgagcgtgc
     1561 ggccgttcag gtcgccgaac tcgtcggcca tcgtcaacag atcggccacc gcctgcaagg
     1621 ggtgggcgtc gtcggacagc aggttgatga tcggcaccca ggccaccttg gccatgcgct
     1681 cgagcacgga gtggtcgaag acccgggccc cgatggcggc gtggtagcag gccagggtgc
     1741 gggccacgtc ttcggccgac tcccgctggt cgatgctgac ctcttcggcc cggatggtca
     1801 ccgggtggcc gccgagatcc ttgacggcca tctccatgga gttgcgggtc cgggccgacg
     1861 gcttctggaa cacgagggcc atgcccttgc gggacagcac ctgcggcggg ttggggtcga
     1921 gggcgagggc gatgacctcg aagagctcgt ccttggtcag gtcgtcgatc tccagcaggt
     1981 gccgcatcag gcgaggatcc cttccaggat cgagagggct tcaccgatct cggcctcgtc
     2041 caccagcagg ctgggggcga agcgcagggc ggtggggctg acgccgttga ccacgagccc
     2101 ggcggcgagc gcggccgtgg ccacctcccg ggcgtcccgg tccgacagct cggccgccag
     2161 gagcaggccg aggccccgca cgcccgtgac gtggggcagg tcgagcaggc cgtcggccag
     2221 gcgcttgccc gcgtcccggg cccgggcggg gacgtcctct cgctccatca cctcgagcac
     2281 cttgcgggcg gcggcggtgg ccaagggctg tc
//
LOCUS       DASTFV010000475         2975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1763972, whole genome shotgun sequence.
ACCESSION   DASTFV010000475 DASTFV010000000
VERSION     DASTFV010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763972"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..478)
                     /gene="dut"
                     /locus_tag="VFP02_08735"
     CDS             complement(44..478)
                     /gene="dut"
                     /locus_tag="VFP02_08735"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET9543153.1"
                     /translation="MIEVPLVRLDPDLAVPAYAREGDAGADLVARDDHDLAPGGGRAR
                     VSTGVSVAIPVGYAGFVLPRSGLALRHGVTCLNTPGLIDAGYRGELQVLLVNTDPSEA
                     FTVRRGERIAQLVIMKVEQAVFAVVDAHDATHRGAGGWGSTG"
     gene            514..1053
                     /gene="orn"
                     /locus_tag="VFP02_08740"
     CDS             514..1053
                     /gene="orn"
                     /locus_tag="VFP02_08740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HET9543154.1"
                     /translation="MLVWMDLEMTGLDHDRDVIVEIATIVTDDELAIVAEGPDLVVHA
                     DEDALARMGDVVRSMHTSSGLLDQIRASTMDLTEAGAQTLAFIREHVPEPRTVPLCGN
                     SIGTDRRFLARWLPEIEEYLHYRSVDVSSVKELAKRWYPESVAKAPGKHGTHRALDDI
                     RESIAELRYYREAVFRSPS"
     gene            1097..2158
                     /locus_tag="VFP02_08745"
     CDS             1097..2158
                     /locus_tag="VFP02_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9543155.1"
                     /translation="MRQEQEEAQPEVTEVAPGILRAQIPINFTGLGHVNMYLLEDEQG
                     WTAMDPGLPGPSTWKAIKDRLRQVGAKVSDVHTVVVTHSHPDHFGSAEKLRREADAEM
                     VAATDFRTFFDILEPDLVESPDDRSAANDAAAIDRLRDRFEMTNPWGGEMPRPPLPGV
                     GPVGKVKRRMARRYFMAPRPNRRLADGEPIRLGSRQFVAVHTPGHTADHLCLFDPDDG
                     VLLTGDHVLPSITPHISGLGSVTEDPLADFVHSLDKVAALDGVGTALPAHGHPFTNLQ
                     ERVDAIKGHHEERLAKLTEISHAVGEANVEVLTKELFSERAWGHMAEAETYAHLEHLR
                     LAGQAESRWDDGLLLYTVD"
     gene            complement(2143..2880)
                     /locus_tag="VFP02_08750"
     CDS             complement(2143..2880)
                     /locus_tag="VFP02_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HET9543156.1"
                     /translation="MSFLPPPPESRAFTHDARVRLGDVSPKGRLRLDGIAKAMQDVAT
                     DDALDGSPGEDAGWVVRRTTIAVGAWPRYREDLTVTTWCSGYGRRWAVRRTTRAGADG
                     GQVDTEALWIHVDPATGRPTPMTPAFFAVWGPGVTDTSVRAKLRLPDHPEGEASSWPL
                     RFSDFDGLAHVNNAAYWLPAEEHLAGRSDLQAPLRAEIEYREGVVRGEEVTVVTADDD
                     DGFLQWWVVDGLVRSSTRVGRVGQSTV"
BASE COUNT          462 a         1125 c          999 g          389 t
ORIGIN      
        1 acctcaccgc cggccgggtc cgcctcacca agaagcacgt cgtttagccg gtcgagcccc
       61 agccgcccgc gccccggtgg gtggcgtcgt gggcgtcgac caccgcgaaa acggcctgct
      121 cgaccttcat gatcaccagc tgggcgatgc gctcgccccg gcggacggtg aaggcctcgc
      181 tcggatccgt gttgacgagg aggacctgga gctcgccccg gtagccggcg tcgatcaggc
      241 ccggggtgtt caggcaggtc acgccgtggc gcagggccag gccgctgcgg gggaggacga
      301 agccggcgta acccaccggg atggcgacgg acacgccggt cgacacccgg gcccggccgc
      361 cgcccggcgc caggtcgtgg tcgtcgcggg ccacgaggtc ggccccggcg tcaccctccc
      421 gggcgtaggc gggaaccgcg aggtcgggat cgagacggac gagcggcacc tcgatcacgg
      481 cgcgcagcgt aagccgcccg tacagtccct ccgatgctgg tgtggatgga cctcgagatg
      541 accggcctcg accacgaccg cgacgtcatc gtcgagatcg ccaccatcgt caccgacgac
      601 gagctggcca tcgtggccga ggggccggac ctggtcgtgc acgccgacga ggacgccttg
      661 gcccgcatgg gcgacgtggt ccgctccatg cacacctcca gcggcctgct cgaccagatc
      721 cgggcctcaa cgatggatct cacggaggcc ggagcccaga cgctggcctt catccgggag
      781 cacgtccccg agccccgcac cgtgcccctg tgcggcaact cgatcggcac cgaccggcgc
      841 ttcctggccc gctggctgcc cgagatcgag gagtacctgc actaccggag cgtggacgtc
      901 tcctcggtca aggagttggc caagcgctgg tacccggagt cggtggccaa ggccccgggc
      961 aagcacggga cccaccgggc cctggacgac atccgggagt ccatcgccga gctgcgctac
     1021 taccgcgagg ccgtcttccg ctctccctca tgacccgcgc gtacttgacc gcacggtcaa
     1081 gtacggtcgc ccgttgatgc gccaggagca ggaggaggcc cagcccgagg tgaccgaggt
     1141 cgcccccggg atcctgcggg cccagatccc gatcaacttc acggggctgg gccacgtgaa
     1201 catgtacctg ctggaggacg agcagggctg gaccgccatg gatcccggcc tcccggggcc
     1261 gtccacctgg aaggcgatca aggaccgcct gcgtcaggtc ggggccaagg tgtccgatgt
     1321 ccacaccgtc gtggtcaccc actcccaccc ggaccacttc ggcagcgccg agaagctgcg
     1381 ccgcgaggcc gacgccgaga tggtggccgc caccgacttc cgcaccttct tcgacatcct
     1441 cgagcccgac ctggtcgagt cccccgacga ccgcagcgcg gcgaacgacg ccgccgccat
     1501 cgaccgcctc cgggaccgct tcgagatgac caacccgtgg ggcggtgaga tgccccggcc
     1561 cccgctcccc ggtgtcgggc ccgtcgggaa ggtcaagcgg cgcatggccc ggcgctactt
     1621 catggccccc cgccccaacc ggcgactggc cgacggcgag ccgatccgct tgggctcccg
     1681 ccagttcgtc gccgtgcaca caccgggcca caccgccgac cacctctgcc tgttcgaccc
     1741 cgacgacggc gtcctcctga ccggtgacca cgtcctgccc agcatcaccc cccacatctc
     1801 cggcctcggg tcggtgaccg aggaccccct ggccgacttc gtccactcgc tggacaaggt
     1861 cgccgcgctc gacggcgtcg gcaccgcgct gcccgctcac ggtcacccgt tcaccaacct
     1921 ccaggagcgg gtggacgcca tcaagggtca ccacgaggaa cggctggcga agctgaccga
     1981 gatctcccat gccgtcggcg aggccaacgt ggaggtcctg acgaaggagc tcttcagcga
     2041 gcgggcctgg ggccacatgg ccgaggccga gacctacgcc cacctcgagc acctacgcct
     2101 ggccggccag gccgagagcc gctgggacga cggcctcctg ctctacaccg tcgactgacc
     2161 gacccggccc acccgggtcg acgaccggac caggccgtcg accacccacc actgcaggaa
     2221 cccgtcgtcg tcatcggccg tgaccacggt gacctcctcg ccccggacga caccctcccg
     2281 gtactcgatc tcggctcgga ggggcgcctg caggtccgat cgcccggcca ggtgctcctc
     2341 ggcgggcagc cagtaggcgg cgttgttcac gtgggccagg ccgtcgaagt cactgaagcg
     2401 gaggggccag gacgaggcct caccctcggg gtggtcaggc agacggagct tggcccggac
     2461 cgaggtgtcg gtcaccccgg ggccccagac cgcgaagaac gccggcgtca tcggcgtcgg
     2521 gcgaccggtc gccgggtcga cgtggatcca gagcgcctcg gtatccactt gaccgccgtc
     2581 ggccccggcc cgtgtcgtcc tccgcacggc ccagcgacgg ccgtacccgg agcaccaggt
     2641 ggtcaccgtg agatcctccc ggtaccgggg ccacgccccg acggcgatgg tcgtgcgccg
     2701 caccacccag cccgcgtcct cccccgggga gccgtcgagg gcgtcgtcgg tggcgacgtc
     2761 ctgcatggcc ttggcgatcc cgtccaggcg gagccgaccc ttggggctga cgtcgcccag
     2821 tcggacccgc gcgtcgtggg tgaaggcccg gctctcgggg ggcggaggca ggaagctcac
     2881 agaccggaaa cgtacgctta cggaaactgt tgccgataaa tacgttgacg gccccgagca
     2941 caccgtgctt tgctggccca gcccgaagac caggc
//
LOCUS       DASTFV010000476         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1770068, whole genome shotgun sequence.
ACCESSION   DASTFV010000476 DASTFV010000000
VERSION     DASTFV010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770068"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VFP02_08755"
     CDS             <1..974
                     /locus_tag="VFP02_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9543157.1"
                     /translation="PIDLGARDTATIGGTVATNAGGNHVLAHGMTRRHLLGIEAVLGT
                     GAVVRHLGGLVKDNTGYDLAALLCGSEGTLGVITELRLALVPPPDETAVALVGLDDLA
                     AAVAVAAEARRHLPDLEAAEVVLADGVTLVRDHLSLAPVLDPEPPVTLLLEVAGPIGA
                     GERLARHLAGVGGIRGSALATEAARRSTLWRVREEHNPAINREGPPHKYDVTLPFDVL
                     DDIVTEARATVAALASEAHLWVFGHVGDGNLHLNVTGLAPDDDRVDEAVLSLVAEAGG
                     SISAEHGIGVAKRRWLSLARSEAEIATFRAIKAALDPDAVLNPGVLL"
     gene            complement(971..1732)
                     /locus_tag="VFP02_08760"
     CDS             complement(971..1732)
                     /locus_tag="VFP02_08760"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET9543158.1"
                     /translation="MAPWAFLDHPGPLAFAHRGGAGDWPENTMPAFEHAVRLGYRYLE
                     TDVHATVDGVLLAFHDDRLDRVTDRQGEIAALPHAEVAAARVDGREPIPLLADLLGSF
                     PRARINIDPKHDGSVGPLIDVLRRTDAVDRVCIGSFSDQRLRTLRDRLGPTLCTSLGP
                     REVARLRLDALSGRVHPSPAGCVQIPEQFRGVSVTNAAVVRAAHRAGLQVHVWTVDQP
                     EAMTRLFDLGVDGIMTDHPAVLKDVLVARGQWVDG"
     gene            1771..2148
                     /locus_tag="VFP02_08765"
     CDS             1771..2148
                     /locus_tag="VFP02_08765"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HET9543159.1"
                     /translation="MAAARIWTAEEADAARPRVGALVERAQAAARVIQEAGPVVPTGH
                     EGNGHTTASAERHELDAVLRELAEDGIVIKDLAQGLIDFPAQSPSGRAYWLCWMVDEP
                     SVAWWHWPEDGFAGRTPLVQPPP"
BASE COUNT          302 a          783 c          813 g          276 t
ORIGIN      
        1 acccgatcga cctcggcgcc cgggacaccg cgaccatcgg ggggacggtg gccaccaacg
       61 ccggaggcaa ccacgtcctg gcccacggca tgacccgacg ccaccttctg gggatcgagg
      121 ccgtgttggg cacgggtgca gtggtccgcc acctcggggg gttggtgaag gacaacacgg
      181 gctacgacct ggccgcgctg ctctgcggct ccgaagggac gttgggagtg atcaccgagc
      241 tccgcctggc cctggtccca ccccccgacg agaccgcggt ggccctggtc gggctcgacg
      301 acctcgcggc cgccgtggcc gtggccgcgg aggcccgtcg ccatcttccc gatctggagg
      361 cggccgaggt cgtcctggcc gacggggtca ccctcgtgcg ggaccacctc tcgctggccc
      421 cggtccttga cccggagccg ccggtcaccc tgctcctcga ggtggcgggg ccgatcggcg
      481 cgggcgagcg gctggcccgg cacctggccg gcgtgggtgg gatccggggc tcggccctgg
      541 ccaccgaggc cgcccgccgc tcgaccctgt ggcgggtgcg cgaggagcac aacccggcga
      601 tcaaccgcga gggcccgccc cacaagtacg acgtgaccct gccattcgac gtgctggacg
      661 acatcgtgac cgaagcccgg gcgaccgtgg ccgccctggc cagcgaggcc cacctctggg
      721 tgttcgggca cgtgggcgat gggaacctgc acctgaacgt gaccggtctc gcccccgacg
      781 acgaccgggt cgacgaggcc gtgctcagcc tggtcgccga agccggcggc agcatcagcg
      841 ccgagcacgg catcggggtg gccaagcgcc gttggctgtc cctggcccgc agcgaggccg
      901 agatcgccac cttccgggcg atcaaggccg ccctcgaccc tgacgccgtc ctcaacccgg
      961 gggtcctcct ctagccgtcg acccactggc cgcgggcgac gagcacgtcc ttcagcacgg
     1021 cggggtggtc ggtcatgatc ccgtcgaccc ccaggtcgaa caaccgggtc atcgcctccg
     1081 gctggtcgac cgtccagacg tggacctgca agccggcccg gtgcgcagcc cgtacgacgg
     1141 cggcgttggt caccgacacc cctcggaact gctcggggat ctgcacgcag cccgccggcg
     1201 acgggtgcac ccgccccgac agcgcgtcga ggcggaggcg ggccacctcc ctgggcccca
     1261 gcgacgtgca gagggtgggc ccgaggcggt cgcgcagggt gcgcagccgc tggtccgaga
     1321 acgagccgat gcacacgcgg tccacggcgt cggttcgccg caggacgtcg atgaggggcc
     1381 ccaccgagcc gtcgtgcttc gggtcgatgt tgatgcgggc ccgggggaag ctccccagca
     1441 ggtccgcgag cagcggaatc ggctcccgcc cgtcgacccg ggccgcggcg acctccgcgt
     1501 gggggagggc cgcgatctcg ccctgtcggt cggtgacccg gtccagcctg tcgtcgtgga
     1561 acgccaggag gacaccgtcg acggtggcgt ggacgtcggt ctccaggtag cggtagccga
     1621 ggcggacagc gtgctcgaag gcgggcatcg tgttctcggg ccagtcgccc gcaccgcccc
     1681 ggtgggcgaa ggccagcgga ccgggatggt cgaggaaggc ccacggcgcc acgaaggcaa
     1741 cctagagggt ggtcgtggga agctggggcg atggcggcgg cccgcatctg gacggccgaa
     1801 gaggccgacg cggcccggcc gcgggtgggc gcactcgtcg aacgggccca ggccgcggcc
     1861 cgcgtcatcc aggaggccgg gccggtggtg cccaccgggc acgagggcaa cgggcacacc
     1921 accgcgtcgg ccgaacgtca cgagctggac gccgtgctcc gggagctggc cgaggacggc
     1981 atcgtcatca aggatctggc gcaaggcctg atcgactttc cggcgcagtc accctcaggc
     2041 cgtgcgtact ggttgtgctg gatggtggac gagccttccg tggcctggtg gcactggccg
     2101 gaagacggct tcgccggccg gactccgctg gtccagccgc cgccgtgaca ccggtacggt
     2161 gaccccgtgg agca
//
LOCUS       DASTFV010000477         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1775867, whole genome shotgun sequence.
ACCESSION   DASTFV010000477 DASTFV010000000
VERSION     DASTFV010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775867"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VFP02_08770"
     CDS             <1..418
                     /locus_tag="VFP02_08770"
                     /inference="COORDINATES: protein motif:HMM:NF025668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HET9543160.1"
                     /translation="APTLSSSTFRAGDTLTVSVPITNTGDRAGAEVVHCYVAPEAPRL
                     ARPPKELKAFAKVRLEAGESTTVDLVLDDRSFAYWDPGQPDWEEVSAKAGAMFGQAAQ
                     ERRSPGWQVDPGRYQLLIGRSSQDLAATVDLEILAN"
     gene            553..1851
                     /locus_tag="VFP02_08775"
     CDS             553..1851
                     /locus_tag="VFP02_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543161.1"
                     /translation="MSKHLAVLVAAGTLLIGCTAGDDTASEDTAGDSATTVDSIAVPT
                     GPAPGVTDDTVKVGVTYVDLASLGEVVNIDHGDYEVAYQAMFDDINASGGINGRMIEP
                     VFAPVSPVGTEGAEAACVQLTQDDPVFVVTGFFLDDAVLCPLEAHQTAVIGSTMTAER
                     LERAEAPWFTAEGSTDLQSDIVRAFADAGEFDGTLGVYAGPGEAAQLNDVVLPLLDEL
                     GVEVAESAAGDASAANAGDITAANAATAVIAERFESSGVDQVLLLGTAGLGWASGTQS
                     LDYRPQLFLTDPNSVLAFAGDPAGNDLSVLDDAVAGNLYGPAENIYALDAMQDCIGVL
                     EDNGGEVPDPATTPEGEDLYVSGFTACDDVTLLRALLEAAGEDLNYGTLAAGAEGLEV
                     QLPSEPEPRTYGPPPAADGDPTPYLYDWDPDAVDFVLRED"
     gene            1870..>2272
                     /locus_tag="VFP02_08780"
     CDS             1870..>2272
                     /locus_tag="VFP02_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET9543162.1"
                     /translation="MSDPGLVVENDVMVAMRDGVRLRADVFRPAGVEDRLPVLVLRNP
                     YSMRDGIWAMLGQAVAQQGYAVVVQSCRGRYGSEGDFSPIHADVEDGYDTVEWAAAQP
                     WSNGAVGMYGLSYSGMAAWAAAIARPPHLVAI"
BASE COUNT          337 a          797 c          809 g          329 t
ORIGIN      
        1 agcgccgacg ctctcgtcat cgacgttccg ggcgggggac acgctgacgg tgtccgtgcc
       61 gatcaccaac acgggcgacc gggccggcgc cgaggtggtg cactgctacg tggcgccgga
      121 ggctccccgg ctggcccgcc cgccgaagga gctcaaggcc tttgcgaagg tccggctgga
      181 ggcgggggag tcgacgacgg tcgacctcgt gctcgacgac cgcagcttcg cctactggga
      241 ccccggccag cccgactggg aggaggtctc ggccaaggcg ggagcgatgt tcggccaggc
      301 cgcccaggag cgccggtccc cgggctggca ggtcgacccg ggccgctatc agctgctgat
      361 cggccgctcc tcccaggacc tcgccgcgac ggtcgacctg gagatactcg cgaactgaac
      421 gcgttccgcg cgcgggtcgt ccttgacgag cgagagcgcg gacgctaaac agttgttcag
      481 cagttcgcag gtggggatgg gagcgctaac agcagcgctg cgatcccacc gtgaagccca
      541 gggggagatc tcatgtccaa gcacctcgcc gtcctcgtgg ccgccggcac actcctgatc
      601 ggatgcacgg ccggggacga cacggcctcc gaagacaccg cgggtgactc ggccaccacc
      661 gtggactcga tcgccgttcc gaccggcccc gcccccggcg tcaccgacga cacggtcaag
      721 gtcggggtga cctacgtcga cctcgcgtcg ctcggggagg tcgtcaacat cgatcacggg
      781 gactacgagg tcgcctacca ggcgatgttc gacgacatca acgcctcggg gggcatcaac
      841 ggccggatga tcgagccggt gttcgccccg gtgagcccgg tcgggacgga aggggccgaa
      901 gcagcctgtg tgcagctcac ccaggacgac ccggtcttcg tcgtcaccgg cttctttctc
      961 gacgacgcgg tgctctgtcc gctggaggcc catcagaccg cggtgatcgg cagcacgatg
     1021 acggccgagc gtctggagcg ggccgaggcc ccctggttca cggccgaggg cagtaccgac
     1081 ctccagagcg acatcgtgcg ggccttcgcc gacgcgggcg agttcgacgg cacgctcggg
     1141 gtgtacgccg gccccggcga ggcggcccag ctcaacgacg tcgtgctccc gctcctcgac
     1201 gagctgggtg tggaggtcgc cgagtcggcc gcaggtgacg cgtcggccgc caacgcgggc
     1261 gacatcaccg cggccaacgc ggcgacggcc gtcatcgccg agcggttcga gtcctcgggt
     1321 gtggaccagg tgctgctcct cgggacggcc ggcctgggct gggccagcgg aacgcagtcg
     1381 ctcgactacc ggccgcagct gttcctgacc gatcccaact ccgtcctcgc ctttgccggc
     1441 gaccctgctg gcaacgacct ctcggtgctc gacgacgcgg tcgccggcaa cctgtacggc
     1501 ccggcggaga acatctatgc cttggacgcg atgcaggact gcatcggggt gctcgaggac
     1561 aacggcggtg aggtccccga tcccgcgacc acccccgagg gtgaggacct ctacgtctcc
     1621 gggttcactg cctgcgacga cgtgacgctg ctccgggcgc tcctcgaagc cgcgggcgag
     1681 gacctgaact acggaaccct cgccgccggg gcggagggtc tcgaggtgca gctgccgagc
     1741 gagcccgagc ctcgtacgta cggtccccct cctgccgccg atggcgaccc cacgccctac
     1801 ctctacgact gggatcccga cgccgtggac ttcgtgctgc gggaagactg agcgggccgc
     1861 ctgtcggaga tgagcgatcc cggactcgtc gtcgagaacg acgtcatggt ggcgatgcgc
     1921 gacggcgtcc gccttcgcgc cgacgtcttc cggcccgcgg gagtcgagga tcgccttccg
     1981 gtgctggtcc tgcgcaaccc gtacagcatg cgggacggga tctgggcgat gctcggccag
     2041 gccgtggccc agcagggcta cgccgtggtg gtccagagct gtaggggccg gtacggctcc
     2101 gagggcgact tctccccgat ccacgccgac gtggaggacg gctacgacac cgtcgagtgg
     2161 gccgcggctc agccgtggtc gaacggcgcc gtcggcatgt acgggctgtc ctacagcggc
     2221 atggccgcgt gggcggcggc gatcgcccga cccccgcacc tggtcgcgat cg
//
LOCUS       DASTFV010000478         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1775976, whole genome shotgun sequence.
ACCESSION   DASTFV010000478 DASTFV010000000
VERSION     DASTFV010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775976"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1845
                     /locus_tag="VFP02_08785"
     CDS             <1..1845
                     /locus_tag="VFP02_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9543163.1"
                     /translation="NVGIRPLIRPDDLDRDFAPLEPERLDELDEALSGTQSDSGIVRF
                     KLWNRQHWVVYSDEQDLRGRWFPSTDLLDESLDGRLGSEITDLDAPEERGEQDLGALL
                     AVYVPLRDDASGEVIGAFEVYLPWSPVAAAIAEDSRDLDLALGAGLLVLYAALYRLVA
                     SATRRLQRQTEENLRQATHDGLTGLPNRGLFTDRLENALAIAGRQGNGLAVVLLDLDG
                     FKEINDALGHASGDEILCELAQRLQHRLRAGDTVARLGGDEFGFLLPTVATDADAVAV
                     AADLQDLLDEPFMVNGMAVHLAASFGVACYPDHGLDAGELVRHADVARYAAKATNTVI
                     EVYGPQFDLHSAERLELAAEVRRALDEDELELWYQPKMDLIDGHIVGVEALLRWSHPT
                     RGIVAPGLFMPVVETTELVRSVTAFVLREAVAQCARWKLDGRDLIVAVNLPARIAHDL
                     RLPGQVADLLARHHLPASALECELTESAVLDHPGRAGQVLSELDRLGVHLSIDDFGTG
                     YASVAYLTDLPIHALKIDQSFVQDVLDDGDGAAIVRFSTDLARRLGLQVVAEGVETRE
                     VLAAVADLGCDMAQGFLLTKPIPAAGLEAWLDDFDPQTLTLPHQAARP"
     gene            1842..>2083
                     /locus_tag="VFP02_08790"
     CDS             1842..>2083
                     /locus_tag="VFP02_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543164.1"
                     /translation="MSRPTSFRSALAPLGAAAVAGLLSTSCGSEAPAAAESPDPVFAL
                     DRDITANIEVPDPDRTAVAPAPDLRILLQTLLAEHS"
BASE COUNT          289 a          814 c          680 g          300 t
ORIGIN      
        1 aacgtcggca tccgcccgct catccggccc gacgacctcg accgcgactt cgcccccctc
       61 gagccggagc gcctcgatga gctcgacgag gcgctgagcg gtacgcagtc cgacagcggg
      121 atcgtccggt tcaagctgtg gaaccgccag cactgggtcg tctactccga cgaacaggac
      181 ctgcgcggac gctggttccc gagcacggac ctgctcgacg agagcttgga tggccgcctc
      241 ggctcggaga tcaccgatct cgatgccccc gaggaacggg gggaacagga cctcggcgct
      301 ctgctcgccg tctacgtgcc cctgcgggac gatgcctccg gggaggtcat cggggccttc
      361 gaggtctacc tgccctggtc ccccgtcgcc gcggcgatcg ccgaggacag tcgggacctg
      421 gacctggccc tcggtgccgg cctgctcgtc ctctacgccg cgctgtaccg cctcgtcgcc
      481 tcggccaccc gccgactgca gcgccagacc gaggagaacc tgcgccaagc cacccacgac
      541 ggcctgaccg gcctgcccaa ccgaggcctc ttcaccgacc gcctcgagaa cgccctggcg
      601 atcgccgggc gccagggcaa cggcctggcg gtcgtcctcc tcgacctcga cgggttcaag
      661 gagatcaacg acgccctggg ccacgcctcg ggtgacgaga tcctgtgtga gctggcgcag
      721 cgattgcagc accgcctgcg ggcgggcgac accgtggccc ggctcggcgg ggacgagttc
      781 gggttcctcc tgcccaccgt ggccaccgac gccgacgcgg tggccgtcgc cgccgacctg
      841 caggacctgt tggacgagcc cttcatggtc aacgggatgg cggtccacct ggccgccagc
      901 ttcggtgtgg cctgctatcc ggaccacggc ctcgacgccg gtgagctcgt gcgccacgcc
      961 gacgtggcca ggtacgcggc caaggccacc aacacggtga tcgaggtcta cggaccccag
     1021 ttcgacctcc acagcgccga gcgtctcgag ctggccgccg aggtccggcg ggccctcgac
     1081 gaggacgagc tggagctctg gtaccagccc aagatggacc tgatcgacgg ccacatcgtc
     1141 ggggtcgagg ccctcctgcg ctggagccac cccacccgcg ggatcgtcgc ccccggcctc
     1201 ttcatgcccg tcgtggagac caccgaactg gtccgcagtg tgacggcgtt cgtcctgcgg
     1261 gaggccgtgg cccagtgcgc ccgctggaag ctcgacggtc gggacctgat cgtcgccgtc
     1321 aacctccccg cccgcatcgc ccacgacctg cgcctgcccg gtcaggtggc cgacctcctg
     1381 gcccgccacc acctccccgc ctccgccctg gagtgcgagc tcaccgagag cgccgtgctc
     1441 gaccacccgg gtcgggccgg acaggtcctg agcgagctcg atcgcctcgg cgtccacctg
     1501 tcgatcgacg acttcggcac cgggtacgcc tccgtggcct acctcaccga cctgcccatc
     1561 cacgccctca agatcgacca gtccttcgtg caggacgtgc tcgacgacgg ggacggggcc
     1621 gccatcgtgc gtttcagcac cgacctggcc cggaggctcg gcctgcaggt ggtcgccgag
     1681 ggggtcgaga cccgcgaggt cctggccgcg gtcgccgacc tgggttgcga catggcccag
     1741 ggcttcctgc tgaccaagcc gatcccggcc gccggtctcg aggcctggct ggacgatttc
     1801 gacccgcaga ccctgaccct tccccaccag gcggcccggc cgtgagccgg cccacctcgt
     1861 tcaggtcggc gctggccccg ctcggcgcgg cggccgtcgc cggcctgctc tcgaccagct
     1921 gtggctccga agcgccggcc gccgccgaga gcccggatcc cgtcttcgct ctcgaccgcg
     1981 acatcaccgc caacatcgag gtgccggatc cggaccggac ggccgtggcg cccgcccccg
     2041 atctgcgcat cctgctgcag accctcctgg ccgagcattc gga
//
LOCUS       DASTFV010000479         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1776211, whole genome shotgun sequence.
ACCESSION   DASTFV010000479 DASTFV010000000
VERSION     DASTFV010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776211"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="VFP02_08795"
     CDS             complement(<1..1298)
                     /locus_tag="VFP02_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543165.1"
                     /translation="MTDLGVLGAGRLGAVDASGGLRPAGAGWTLGWWIGAEDRWHVPP
                     VETAVRQSLVGSTPVVETRVRVVGGDVVHHAYAVTGPGGVAMLVAEITNETPVPVAIA
                     LVVDGAGSVDLDGEVATVDGHHQVRLPRPASQWVAPVAFLPLPHKATLRVVMARGEAA
                     DPAELPDAERVAAGWRAQADASSSWSLPEPIISAAADAARGFLLVHDEPDLLTTALSA
                     EARHLLGLRDERGTRADLAARQRITGAFEDGTGSLAATGQALVALGSAPDPALIGPVA
                     KAGHWIERKRRVRRHRKDALRAGLLPPGPQPLVLGAADQTYLDDWWSVAGLVRAAGLL
                     DAEGQLEASVDAWRFARGLAADVDRSIAAVLASDLLAQGGESEARTGAFPAGPGRALD
                     AGVVGVVVAAALGALDPEAPAVVATLDLVRAELVSEAGGVR"
     gene            complement(1300..>2266)
                     /locus_tag="VFP02_08800"
     CDS             complement(1300..>2266)
                     /locus_tag="VFP02_08800"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET9543166.1"
                     /translation="GSWGTTVAHLAAHNTPTTLWARRADVAEEINTSHTNSRYLADFT
                     LHDGLVATDDLSAAVACADVLVMGVPSSGFRETLKEVANDLRPWVPVVSLAKGLEQGT
                     NLRMTQVIQEELPGHPYGVLTGPNLAKEILSEQAAAAVIAMSDATVAARLQDLFATDL
                     FRVYTNQDVIGSELGGALKNVIALASGMADGLGAGDNTRAAVITRGLAELTRLGVAMG
                     GQPETFAGLAGMGDLLATCISSQSRNRHVGEELGKGKSIEQIVEEMNQVAEGVKSARV
                     VLELAEQYGVDMPITAQVASVVGGTAPAENAYRGLLKRTVSSERR"
BASE COUNT          290 a          836 c          845 g          295 t
ORIGIN      
        1 cggacgccac ccgcctcgct caccagctcg gcccgcacca ggtccagcgt ggccaccacc
       61 gccggcgcct ccgggtcgag cgcccccagt gccgcggcta cgaccacgcc gaccaccccc
      121 gcgtccaggg cccgccccgg gccggcggga aacgcacccg ttctggcctc cgattcaccg
      181 ccctgggcga gtagatcaga ggccagaacg gcggcgatcg agcggtcgac gtcggcggcc
      241 aggccgcggg cgaagcgcca ggcgtcgacc gaggcctcga gctgaccttc ggcgtcgagc
      301 agtccggcgg cccggaccag gccggcgacg gaccaccagt cgtccagata ggtctggtcg
      361 gcggcgccga gcacgagcgg ttgcggcccg ggggggagta gccccgcccg caaggcgtcc
      421 ttgcggtggc gacggacccg gcgcttgcgt tcgatccagt gcccggcttt ggccaccggc
      481 ccgatcaacg ccgggtcggg ggccgagccc agggcgacca gggcctggcc ggtcgccgcc
      541 aggctgcccg tcccgtcctc gaaggccccg gtgatccgct ggcgagcagc caggtcggcc
      601 cgggtccccc gctcgtcccg gaggccgagg aggtgccggg cctcggccga cagcgccgtg
      661 gtgagcaggt cgggttcgtc gtggacgagg aggaagcccc gggcggcatc ggccgccgcg
      721 ctgatgatcg gctcggggag cgaccaggac gagctggcgt cggcctgcgc ccgccagccg
      781 gcggcgaccc gctcggcgtc cggaagctcc gcggggtcgg cggcctcgcc gcgggccatg
      841 accacccgca gcgtcgcctt gtggggaagg gggaggaagg cgacgggcgc gacccactgg
      901 ctggcggggc ggggcaggcg cacctggtgg tggccgtcga cggtcgccac ctcgccgtcg
      961 aggtcgaccg agcccgcccc gtcgacgacc agggcgatgg ccaccggcac gggcgtctcg
     1021 ttggtgatct cggccaccaa catggccacg ccacccggcc cggtgacggc gtacgcgtgg
     1081 tggaccacgt cgccgccgac gacccgcaca cgggtctcga cgaccggggt cgacccgacc
     1141 agggactggc gcacggcggt ctcgaccggt gggacgtgcc agcggtcctc cgctccgatc
     1201 caccagccca gcgtccagcc cgctccggcc gggcgcagcc cgccgctggc gtcgaccgcg
     1261 ccgaggcggc cggcgccgag gaccccgaga tccgtcacgt cagcgccgtt cggaggagac
     1321 cgtgcgcttg agcagccccc ggtaggcgtt ctcggcgggc gccgtcccac ccacgacgct
     1381 ggccacctgg gcggtgatgg gcatgtccac gccgtactgc tcagccagct cgagcacgac
     1441 ccgggccgac ttcacgcctt ccgcgacctg gttcatctcc tcgacgatct gctcgatgga
     1501 cttgcccttg cccagctcct cgcccacgtg gcggttgcgg ctctggctcg agatgcacgt
     1561 ggcgaggagg tctcccatgc ccgccaggcc cgcgaaggtc tccggctggc cgcccatggc
     1621 gacgccgagg cgggtcagct cggccaagcc ccgggtgatg accgcagccc gggtgttgtc
     1681 gcctgctccg agcccgtccg ccatgcccga ggcaagggcg atcacgttct tcagcgcgcc
     1741 gcccagctcg ctgccgatga cgtcctggtt cgtgtagacg cggaagaggt cggtggcgaa
     1801 caggtcctgc aggcgggcgg cgacggtcgc gtcggacatg gcgatcacgg cggccgcggc
     1861 ctgctcggag aggatctcct tggccaggtt gggcccggtc agcactccgt aggggtggcc
     1921 gggcagctcc tcctggatga cctgggtcat gcgcaggttg gtcccctgtt cgaggccctt
     1981 ggccaagctg accacgggaa cccacgggcg caggtcgttg gccacctcct tcagggtctc
     2041 ccggaacccg gagctgggaa cgcccatgac gaggacgtcg gcgcaggcca cggccgcgga
     2101 caggtcgtcg gtggccacca ggccgtcgtg gagggtgaag tccgccaggt agcggctgtt
     2161 ggtgtggctg gtgttgatct cctcggccac gtcggcgcgg cgggcccaga gcgtggtcgg
     2221 cgtgttgtgc gcggcgaggt gggcgaccgt cgtcccccac gacccg
//
LOCUS       DASTFV010000480         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1777578, whole genome shotgun sequence.
ACCESSION   DASTFV010000480 DASTFV010000000
VERSION     DASTFV010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777578"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VFP02_08805"
     CDS             complement(<1..892)
                     /locus_tag="VFP02_08805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9543167.1"
                     /translation="MDGETVHVRPISPEDADRLRAFHEGLSPESIQYRFFSPHPTLSA
                     TEVEHFTTVDHHDRVALVATRGERIVAVARYERLKDPDTAEAAFVVADEFQHRGLGAL
                     LLEHLAAAAREHGITRFEASVLTANRAMLNVFRAAGFDEVTHVESSVIEVELIIEPSE
                     RSLAAAEHREHIAEAASVARLLTPRSVAVIGVGRNPASVGHVVLTNLQRAGFAGPIYP
                     VNPRADEIAGLTVYPSLAAVPGPVDLAVVAVPSGAVMDVVRDAIAKEVHGLVVLTAGF
                     GEVGDHATQDGLRDLARGNGL"
     gene            complement(960..2297)
                     /locus_tag="VFP02_08810"
     CDS             complement(960..2297)
                     /locus_tag="VFP02_08810"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9543168.1"
                     /translation="MLTHHHEAPGLQLHLAGVPVRLEWTFLVFTVLIGATLGELTLIV
                     AWVVVVTGSILVHELGHAAAFRVWGVGSRIVLHTMGGVTIPSSGLPTRWSRIAVSLAG
                     PVSGLVLLGLPAVIAERSMDLSAPWDDVVAFAVWVNIGWALINLLPILPLDGGHVAHE
                     LIDAGTGGRGEVPARWLSIVVAAAMGLWALTQGFVFGALFAFFFLSDNVRALQGRRET
                     GAVANLRPAAEALEQGDADAAIGLAGGVLETARDTQAKAMALDLTAWAHLARRDTAAA
                     AAALARYPDDVEPSGHLRAFLIETVDAERVNATVDAWLAHRYLPAWVYTRCLVEAGLV
                     DVVGDRLLASRADGAERARLAFQHTLFVAGQMDASARFGERLVDAGGTDPIVAYNVAC
                     AHSRAGRTDPAVRWLGTAIDQGFTNRELLDGDPDLARVRTDPRFAMLRNRLPG"
BASE COUNT          388 a          932 c          949 g          336 t
ORIGIN      
        1 gaagcccgtt gccccgggcc aggtcgcgca acccgtcctg ggtggcgtgg tcgcccacct
       61 cgccgaaacc ggcggtgagc acgaccaggc cgtgcacctc cttcgcgatg gcgtcccgca
      121 ccacgtccat caccgccccc gagggaacag ccaccaccgc caggtcgacc gggccgggca
      181 cggcggccag gctcgggtag acggtcagcc cggcgatctc gtcggcccgg gggttcaccg
      241 gatagatggg ccccgcaaac ccggctcgct gcaggttggt gagcacgacg tggcccacgc
      301 tggcggggtt ccgcccgacg ccgatgaccg ccaccgatcg gggcgtcaac agccgggcga
      361 ccgatgccgc ttccgcgatg tgctcccggt gctcggccgc cgccaacgac cgctccgagg
      421 gctcgatgat cagctcgacc tcgatcaccg acgactcgac gtgggtgacc tcgtcgaagc
      481 cggccgcgcg gaagacgttg agcatcgccc ggttggcggt cagcaccgag gcctcgaagc
      541 gggtgatccc gtgctcccgg gccgccgccg ccaggtgctc caacaggagc gccccgagtc
      601 cccggtgctg gaactcgtcg gccaccacga aggcggcttc ggccgtatcg gggtccttca
      661 gccgttcgta gcgagccacc gccacgatcc gctcgccccg cgtggccacc agcgccaccc
      721 ggtcgtggtg gtcgaccgtg gtgaagtgct cgacctcggt ggcgctcagc gtggggtggg
      781 gcgagaagaa ccggtactgg atcgactctg gcgacagtcc ctcgtggaag gcccgcagcc
      841 gatcggcgtc ctcgggcgag atgggccgga cgtgcaccgt ctcgccgtcc atcaggacga
      901 catcggcttc ccagtgggac ggcgcggggc tcgggatgaa gtggagggta gatcggggtt
      961 cagcccggca ggcggttgcg gagcatggcg aacctcgggt cggtgcggac ccgggccagg
     1021 tccgggtcac cgtccaggag ctcccggttg gtgaagccct ggtcgatcgc cgtgccgagc
     1081 catcgcaccg cggggtcggt ccggccggcc cgggaatgtg cgcaggccac gttgtaggcc
     1141 acgatcgggt cggttcctcc ggcgtcgacc agacgctccc cgaagcgggc ggacgcgtcc
     1201 atctggccgg ccacgaacag ggtgtgctgg aaggccagcc gggcccgctc ggccccgtcg
     1261 gcccgagagg ccaggagccg gtcaccgacc acgtcgacga gcccggcctc gaccaggcac
     1321 cgcgtgtaca cccacgcggg gaggtagcgg tgggcgagcc aggcgtcgac ggtggcgttg
     1381 acccgctcgg cgtcgacggt ctcgatcagg aaggcgcgaa ggtggccact gggctcgacg
     1441 tcgtccgggt accgagcgag ggcggccgcc gccgcagcgg tgtcgcgccg ggcgaggtgg
     1501 gcccacgcgg tgaggtcgag ggccatcgcc ttcgcctgcg tgtcccgcgc cgtctccagc
     1561 acccccccgg cgagtccgat ggcggcatcg gcatcgcctt gctccaaggc ttcggccgcg
     1621 ggccggaggt tcgcgacggc cccggtctcc cgacgcccct gcagggcccg gacgttgtcg
     1681 gacaggaaga agaaggcgaa cagggccccg aacacgaatc cctgggtcag ggcccagagg
     1741 cccatcgccg cggcgaccac gatcgagagc cagcgggccg gcacctcacc tcgaccgccg
     1801 gtgccggcgt cgatcagctc atgggccacg tgaccgccgt cgaggggcag gatcggcagc
     1861 aggttgatca gggcccagcc gatgttcacc cagacggcga aggcgacgac gtcgtcccac
     1921 ggcgccgaca ggtccatcga ccgttcggcg atgaccgcag ggaggccgag cagcaccagc
     1981 cccgagacgg gcccggccag gctcacggcg atgcgactcc agcgcgtcgg caggcctgag
     2041 gaggggatgg tgaccccgcc catggtgtgg agcacgatcc gggagccgac gccccagacc
     2101 cggaaggcgg cggcgtggcc cagctcgtgg acgaggatcg atccggtcac cacgacgacc
     2161 caggccacga tcagggtgag ctcgcccaac gtcgcaccga tcagaacggt gaacaccagg
     2221 aaggtccact cgaggcggac ggggaccccg gcgaggtgga gctggagccc cggcgcctcg
     2281 tggtggtggg tcagcatgcc ccgcgtccgg acggtttcag gttgtggttc cggtccggca
     2341 ccccggcacc attgcacgag aaatcggact ggctccgcgc cgtcccgtcc acgggtcccc
     2401 gccaaagcgt cgtcggtagg ctgatgtccg actgtacgcg gaccggtcgc cgtcgccccg
     2461 acggttctga ccggtccggt tgggggggcg atggaccgcc accgggatgg cgaggtgccc
     2521 atgacctcac ccgcggccgg cctcgtccag cgcatgggtg cactcgccgc cgccgccgtg
     2581 ccctatgtcc acgccaccgt ggtcc
//
LOCUS       DASTFV010000481         5128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1781099, whole genome shotgun sequence.
ACCESSION   DASTFV010000481 DASTFV010000000
VERSION     DASTFV010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781099"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..945)
                     /locus_tag="VFP02_08815"
     CDS             complement(13..945)
                     /locus_tag="VFP02_08815"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9543169.1"
                     /translation="MTTVLSQMGERITGEIAAAFPDLDVRSIGTGEALEDDAAGEVLL
                     TSHWFAQDFPTLFGRGVRWVHNFGTGVDHFPFDLLLPDQVLTCSRGAGAGPISEWVIT
                     QMLAFEKRLPGSWITEPPEHWNNADLGTLKGSHVGIIGYGGIGEAVARRALPFGSEVR
                     AYRRRAIPADNPGVDVVTDLPALAEWCDHLVVTAPSTPETHHLVGPAVLDVVGPGAHL
                     VNIARGALVDQEALRTALDDGRVARASLDTVEPEPLPEGHWLYAHPQVNLSAHISWSS
                     PEGYDRILGIFLEELARWESGETLANEVDRDAGY"
     gene            complement(959..2611)
                     /locus_tag="VFP02_08820"
     CDS             complement(959..2611)
                     /locus_tag="VFP02_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HET9543170.1"
                     /translation="MSTTGERTFNLADLFEQAVDAYPEREYLVVGDQRRTYAQMEERA
                     NRLAHDLAEHGVEPGDHVGIYAHNSAEWVETLWAVFKIRATWININYRYVEGELSYLF
                     GNSDLKALVYEQGFGARVAACVDALPQLVHALVIDDGSGADVGLPDAVPYEDALAAAS
                     PERDFGPRSPDDRYILYTGGTTGMPKGVVWRHEDVFMALGGGIDPMTKVRATKPEEII
                     AKAYPDPITFFPIAPLMHGATQWGLMSQAMTGNRVVLVANFDADECWELIQREGINSM
                     MITGDAMARPLIETLQANRDRYDVSSLFSLSSSAAVFSPSVKDQFFEVFPDLILTDAI
                     GASESGNNGMVVVGKGNTAMKGGPTVTAMPDSVVIDEDFNIVQPGSGVVGKVARFGNI
                     PLGYYNDEVKTAETFITGPDGTRYAVPGDFATVEEDGSITLLGRGSVSINSGGEKIYP
                     EEVEAAVKSHPAIYDATVIGVKDERWGQRVAAVVQLRAGAELASVKDLQEHCRKEIAG
                     YKVPRQVHFVDAVSRAPSGKPDYRWAAEVAAGNPELIDEDFA"
     gene            complement(2656..2847)
                     /locus_tag="VFP02_08825"
     CDS             complement(2656..2847)
                     /locus_tag="VFP02_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543171.1"
                     /translation="MPFIVTFGLSTAVVGGICGYLFGKRWKNRPVLGAVLCFVIPILG
                     LIILWYVPPKAPGPMTPAT"
     gene            complement(2890..4458)
                     /locus_tag="VFP02_08830"
     CDS             complement(2890..4458)
                     /locus_tag="VFP02_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HET9543172.1"
                     /translation="MSDEKERLYPGTWAERRPDHPAVIMAGSGEVITYAGFDEMACRL
                     ANLYRSIGLQPGHHVAMCLENRVEFLALAWGARYAGLYYTAISSRLTAEELGYIVEDS
                     GSRVLITSPAKADQAAELMDQLGGLDLLASVGGPVDGFEPLEDLLAAQSSEEPADAVE
                     GHAMLYSSGTTGRPKGVKAPFTGPKLGTAEAMTSLITLVFGADAQSVYLSPAPLYHAA
                     PMAFCMNFLRIGATVVVMEHFDAVEALALIERYRATHSQWVPTMFVRMLKLPPEERTA
                     HDLSSMKVAVHAAAPCPVPVKEQMIEWWGPVIHEYYAGTEGNGFCYCNSEEWLAHKGT
                     VGRGLTAPVLILDEEGREVPTGEEGTIWFGATPAGRIFEYHNDPEKTAGSYRDDGAST
                     LGDIGKLDDDGFLYLTDRKANMIISGGVNISPQETENVLIVHPAVADAAVIGVPNEEF
                     GEEVKAVVQLVDPAAAGPEMERALIAHCRDHLADVKCPRSVDFRDELPRHPTGKLYKR
                     LLKDEYWAGHDSRI"
     gene            4529..>5128
                     /locus_tag="VFP02_08835"
     CDS             4529..>5128
                     /locus_tag="VFP02_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543173.1"
                     /translation="MSVGPDRTPVIAGIALSDYPIAPELDGVQHHVLAMQRVLEDSGV
                     AKADIDGYVSAGGGGMMVDDPVTMSEYLCVDHRWIDGTMTGGSSFEFHVQHAAAAIRD
                     GRAHTILVTYGSDQLSRMGRTLGTGSFRAGQSQAVPGPLQFEAPYGTSLVGSYAMVAQ
                     RHMHEFGTTSEQLAEIAVGVREYAQLNPLARYRDDLTVED"
BASE COUNT          760 a         1671 c         1884 g          813 t
ORIGIN      
        1 ggaacgaccc gctcagtacc cggcgtcccg gtcgacctcg ttggccaggg tctcgccgga
       61 ttcccagcgg gccagttcct cgaggaagat gccgaggatg cggtcgtagc cctcgggcga
      121 gctccacgag atgtgggcgc tgaggttgac ctgcgggtgt gcgtagagcc agtggccctc
      181 gggcagcggc tcgggctcga ccgtgtccag tgaggcccga gccacccgtc cgtcgtcgag
      241 tgcggtgcgc agggcctcct ggtcgaccag ggcgcctcgg gcgatgttga cgaggtgggc
      301 gccggggccg actacgtcga gaacggccgg gccgaccagg tggtgggtct cgggggtcga
      361 gggcgcggtg acgacgaggt ggtcgcacca ctcggccagg gccggcaggt cggtgacgac
      421 gtcgacgccc ggattgtcgg ccgggatcgc acgtcggcgg taggcccgaa cctcactgcc
      481 gaagggcagg gcccggcggg ccacggcctc accgatgccg ccgtagccga tgatcccgac
      541 gtggctgccc ttgagggtgc cgaggtcggc gttgttccag tgctcaggcg gctcggtgat
      601 ccaggagccg ggcagacgct tctcgaaggc cagcatctgg gtgatgaccc actcggagat
      661 gggcccggcc ccggcgccgc gggagcaggt caggacctgg tcggggagca gcaggtcgaa
      721 cgggaagtgg tcgaccccgg tgccgaagtt gtggacccag cggacgcctc gaccgaagag
      781 cgtcgggaag tcctgggcga accagtgcga ggtgagcagg acctcgcccg cagcatcgtc
      841 ctcgagtgcc tcaccggtac cgatcgaccg aacgtcgaga tccgggaagg cggccgcgat
      901 ctcgccggtg atgcgctcgc ccatctggga gaggacggtg gtcacgggcg gaagcctctc
      961 aggcgaagtc ctcgtcgatc agttcggggt tgccggcggc gacctcggcc gcccaccggt
     1021 agtcgggctt gccactcggg gcccgggaca cggcgtccac gaagtggacc tggcggggga
     1081 ccttgtagcc ggcgatctcc ttgcggcagt gctcctgcaa gtccttgacc gaggccagct
     1141 cggccccggc ccggagctgc acgacggcgg cgacccgctg gccccagcgc tcgtccttca
     1201 cgccgatgac cgtggcgtcg tagatcgccg ggtgggactt gacggcggcc tcgacctctt
     1261 cggggtagat cttctcgccg ccggagttga tggagaccga gccccggccg aggagggtga
     1321 tggagccgtc ctcctcgacg gtggcgaagt caccggggac cgcgtagcgg gtgccgtcgg
     1381 gaccggtgat gaaggtctcg gccgtcttga cctcgtcgtt gtagtagccg agcgggatgt
     1441 tgccgaagcg ggccaccttg cccacgacac cggagccggg ctggacgatg ttgaagtcct
     1501 cgtcgatgac gacggagtcg ggcatggcgg tgaccgtcgg gccacccttc atggccgtgt
     1561 tgcccttgcc cacgacgacc atgccgttgt tgccggactc ggaggcgccg atggcgtcgg
     1621 tcaggatcag gtcggggaag acctcgaaga actggtcctt caccgaaggg gagaagaccg
     1681 ccgcgctgct ggacagcgag aacaacgagg agacgtcgta gcggtcccgg ttggcctgca
     1741 gggtctcgat caggggccgg gccatggcgt cgccggtgat catcatcgag ttgatgccct
     1801 cgcgctggat gagctcccag cactcgtcgg cgtcgaagtt ggccacgagc acgactcggt
     1861 tgccggtcat ggcctggctc atgaggcccc actgggtggc accgtgcatg aggggggcga
     1921 tggggaagaa ggtgatgggg tcggggtagg ccttggcgat gatctcctcg ggcttggtgg
     1981 cccggacctt cgtcatgggg tcgatgccgc cgccgagcgc catgaagacg tcctcgtggc
     2041 gccagacgac gcccttgggc atcccggtgg tgccgcccgt gtagaggatg tagcggtcgt
     2101 cgggggagcg ggggccgaag tcgcgctccg gggaggcggc ggccagggcg tcctcgtagg
     2161 gcacggcgtc gggcaggccg acatcggcgc cggagccgtc gtcgatgacg agggcgtgga
     2221 cgagctgggg cagcgcgtcg acacaggccg cgacccgggc tccgaagccc tgttcgtaga
     2281 cgagggcctt caggtcgctg ttgccgaaga ggtaggagag ctcgccctcg acgtagcggt
     2341 agttgatgtt gatccaggtg gcccggatct tgaagacggc ccagagggtc tcgacccact
     2401 cggcggagtt gtgggcgtag atgccgacgt ggtcacccgg ctcgacgccg tgctcggcca
     2461 ggtcgtgagc cagccggttg gcccgctcct ccatctgggc gtaggtccgg cgctggtcgc
     2521 cgaccaccag gtactcccgc tccgggtagg cgtccaccgc ctgctcgaac aggtcagcca
     2581 ggttgaaggt gcgttccccc gtcgtgctca cgcccccaaa ctagaacacg ttccagtccc
     2641 tgagaaacca acacgtcagg tcgcgggcgt catcgggccg ggcgccttcg gcgggacgta
     2701 ccagaggatg atcagcccga ggatggggat cacgaagcag aggacggcac cgagcacggg
     2761 ccggttcttc caccgcttgc cgaagaggta gccgcagatc ccacccacga ccgcagtgct
     2821 gagaccgaag gtgacgatga acggcatgcg tgctctcttt cgagtgggcg gaagctgcag
     2881 cctcccacgt cagatgcggc tgtcgtggcc ggcccagtac tcgtccttca gcaggcgctt
     2941 gtagagcttg ccggtggggt ggcgggggag ctcgtcccgg aagtcgacgg agcgggggca
     3001 cttgacgtcg gccaggtgat cccggcagtg ggcgatgagc gcccgctcca tctccgggcc
     3061 ggcggccgcc gggtcgacga gttggacgac ggccttgacc tcctcgccga actcctcgtt
     3121 cgggaccccg atgaccgcgg cgtcggctac tgcggggtgc acgatcagga cattctcggt
     3181 ctcctgggga gagatgttca ccccgcccga gatgatcatg ttggccttgc ggtcggtgag
     3241 gtagaggaag ccgtcgtcgt ccagcttgcc gatgtcgccg agggtgctgg ccccgtcgtc
     3301 ccggtagctg ccggcggtct tctcggggtc gttgtggtac tcgaagatcc gcccggccgg
     3361 ggtcgccccg aaccagatcg tgccttcctc gcccgtgggc acctcgcggc cctcctcgtc
     3421 gaggatgagg accggtgcgg tcaggccccg gccgaccgtg cccttgtggg ccagccactc
     3481 ctcgctgttg cagtagcaga agccgttgcc ctcggtgccg gcgtagtact cgtggatgac
     3541 cgggccccac cactcgatca tctgctcctt gactggcacc gggcagggcg cagcggcgtg
     3601 gacggcgacc ttcatcgagc tcaggtcgtg ggccgttcgc tcctcggggg gcagcttgag
     3661 catgcgcacg aacatggtcg ggacccactg gctgtgcgtg gcccggtagc gctcgatgag
     3721 ggcgagggcc tccacggcgt cgaagtgctc catgacgacg acagtcgccc cgatgcggag
     3781 gaagttcatg cagaacgcca tcggggcggc gtggtacagc ggggcgggag acaggtagac
     3841 ggactgggcg tcggcgccga agaccagcgt gatcagcgac gtcatggcct cggcggtgcc
     3901 gagcttggga ccggtgaagg gggccttgac ccccttgggc cgaccggtgg tgcctgagga
     3961 gtagagcatc gcgtgaccct cgacggcgtc ggccggttcc tcggaggact gcgcggccag
     4021 caagtcctcc agcggctcga agccgtcgac cggcccgccc accgacgcca gcaggtccag
     4081 cccgccgagc tgatccatga gctccgccgc ctggtcggcc ttggccggtg acgtgatcag
     4141 gacccggctg ccggagtcct cgacgatgta gcccagctct tccgccgtga gccgggagct
     4201 gatggccgtg tagtagaggc cggcgtagcg ggcgccccag gccagggcca ggaactcgac
     4261 ccggttctcc aggcacatcg ccacgtggtg accgggctgc aggccgatcg accggtaaag
     4321 gttggccagg cggcacgcca tctcgtcgaa cccggcgtag gtaatgacct cgcccgagcc
     4381 cgccatgatc accgccggat ggtccggccg ccgctcggcc cacgtccccg ggtacagccg
     4441 ttccttctca tcgctcacgc gcgtcgcccc ccggtcgctg gtgtccgccg ttctggcgcc
     4501 cattctggcc cggctacggt cccgcgtcgt gagcgtgggg cctgaccgga cgccggtgat
     4561 cgccgggatc gcgctgtcgg actatcccat cgcgccggag ctcgacgggg tccagcacca
     4621 cgtgctcgcc atgcagcggg tcctcgagga cagcggcgtg gccaaggccg acatcgacgg
     4681 ctacgtgtcc gcgggcggcg gcgggatgat ggtcgacgac ccggtgacca tgtccgagta
     4741 cctctgtgtc gaccaccgct ggatcgacgg gaccatgacc ggggggtcgt ccttcgagtt
     4801 ccacgtgcag cacgccgccg ccgccatccg ggacggtcga gcccacacga tcctcgtcac
     4861 ctacggctcg gaccagctga gccgcatggg tcgaaccttg gggacgggca gcttccgggc
     4921 gggtcagagc caggccgtcc ccggtccgct gcagttcgag gcgccgtacg ggacctcgct
     4981 ggtcgggtcc tacgccatgg tcgcccagcg ccacatgcac gagttcggca cgacgtcgga
     5041 gcagctggcc gagatagccg tgggcgtccg ggagtacgcc cagctcaacc ctctggcccg
     5101 ctatcgggac gacctcaccg tggaggac
//
LOCUS       DASTFV010000482         2004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1783590, whole genome shotgun sequence.
ACCESSION   DASTFV010000482 DASTFV010000000
VERSION     DASTFV010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783590"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="VFP02_08840"
     CDS             <1..1540
                     /locus_tag="VFP02_08840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET9543174.1"
                     /translation="HYVGGSLRALDLVDYPTYMIAANALPPVPAVYGDGWREEWRRRV
                     DGTEPWILRWLAEQTDSSYWRKGSLREGTRPPESDQGYDRIEAATMLVAGWADGYRNN
                     TFRTFERLTCPKRLLAGPWSHMSTASSLPGPHIDLVPELIAWFGHHLRGDDNGVDTAP
                     PIQVYVRDAVEPEPDLAEHPGEWWALPSWPPPEHATASFRVDGEGALLVPEAHDVGEA
                     AWNSCAASLPWGQPTDQRADEARSLLLRWPAPAEGIDVLGHPRLHLRARCDRPVAALS
                     LKLADGLPGGSSALVTRGFLNLTHRESSVEPTEVPFGEWLDLTIDLEATTWHFDPGHE
                     VRLAIARSDWPNAWPAPEIGVIEIDQASISLDLPIAERPDLPYPHFVDPPPDGDNHAP
                     PAGDAPQPVTRWWIEHDVLRRRRTARDEHGDVYDGRYGAHIVDTYTGATDVSTVDPAD
                     AGAEGTYRCELTWPEVAVVAEARLSVRSTVDEYEVEIDLDVTEDGQPFARRRWHEVIP
                     RHLQ"
     gene            complement(1518..>2004)
                     /locus_tag="VFP02_08845"
     CDS             complement(1518..>2004)
                     /locus_tag="VFP02_08845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9543175.1"
                     /translation="IVADEPVSALDASVRGEILALMLNLVQETGISILAITHDLGLAW
                     NIADRVAVMYLGRIVELGTTEEVLEHPQHPYTQALLSVVPESKKLRQEILTGEPPDPK
                     NIPAGCRFRPRCPLVQSGEAEKLGILSRCEQDDPRLLPIGAPHQAACHATNRATAGGG
                     G"
BASE COUNT          294 a          774 c          666 g          270 t
ORIGIN      
        1 ccactacgtc gggggctcgc tgcgagccct cgacctcgtc gactacccga cctacatgat
       61 cgcggccaac gccctgcccc cggtccccgc cgtctacggc gacggctggc gggaggagtg
      121 gcgacggcgg gtcgacggga ccgagccctg gatcctgcgc tggctcgccg agcagacgga
      181 ctcgtcgtac tggcgcaagg gctccctgcg ggagggcacc cgaccgccgg agtcggacca
      241 gggctacgac cgcatcgagg ccgccaccat gctcgtcgcc ggatgggccg acgggtaccg
      301 caacaacacc ttccgcacct tcgaacgcct gacctgcccc aagcgcctgc tggccggtcc
      361 gtggagccac atgtccaccg cgtcctcgct gcccggcccc cacatcgatc tcgtacccga
      421 gctcatcgcc tggttcggac accacctgag gggcgacgac aacggggtcg acaccgcccc
      481 gcccatccag gtctacgtac gggacgccgt cgagcccgag cccgacctgg ccgagcaccc
      541 gggcgagtgg tgggccctgc cgtcgtggcc accgcccgag cacgccacgg cgtcgttccg
      601 ggtcgacggc gagggggcgc tgctcgtccc cgaggcccac gacgtcggcg aggcggcgtg
      661 gaactcctgt gcggcttcgc tgccatgggg acagcccacc gaccagcgag ccgacgaggc
      721 ccgctcactc ctgctgcgct ggcccgcgcc cgcggaggga atcgacgtcc tcggccatcc
      781 tcggctccac ctgcgggccc ggtgcgaccg cccggtcgcc gccctctccc tcaagctggc
      841 cgacggcctg cctggtggct cgtcagcact ggtcactcgc ggcttcctga acctgaccca
      901 ccgggagtcc tcggtcgaac ccaccgaggt ccccttcggt gagtggctgg acctgaccat
      961 cgacctcgaa gccacgacct ggcacttcga ccccggccac gaggtccgtc tggccatcgc
     1021 ccggtccgac tggcccaacg cctggcccgc ccctgagatc ggcgtgatcg agatcgacca
     1081 ggcctcgatc agcctcgacc tgcccatcgc cgagcgccct gacctgccct acccccactt
     1141 cgtcgacccg cccccggacg gcgacaacca cgcacccccg gccggcgacg cgccccagcc
     1201 ggtgacccgc tggtggatcg agcacgacgt cctccggcgt cgccgcaccg cccgggacga
     1261 gcacggcgac gtctacgacg gccgctacgg cgcccacatc gtcgacacct acaccggcgc
     1321 gaccgacgtc agcaccgtcg accccgccga cgccggcgcc gagggcacct accgctgcga
     1381 gctcacctgg cccgaggtgg ccgtcgtcgc cgaagcccgc ctctcggtcc ggagcaccgt
     1441 tgacgagtac gaggtcgaga tcgacctcga cgtcaccgag gacggccagc ccttcgcgag
     1501 acgccgctgg cacgaggtca tcccccgcca cctgcagtag cgcggttggt ggcgtggcac
     1561 gccgcttgat ggggggcacc gatgggcagg agccggggat cgtcctgctc gcaccgagac
     1621 aggattccca gcttctccgc ctcgccggac tggaccaacg ggcagcgggg gcggaagcga
     1681 cagccggcgg ggatgttctt cgggtcaggg ggctcgccgg tcaggatctc ctggcggagc
     1741 ttcttcgact cggggacgac cgacaacagg gcctgcgtgt aggggtgctg ggggtgctcg
     1801 aggacctcct ccgtggtccc cagctccacg atgcggccga ggtacatgac cgccactcgg
     1861 tcggcgatgt tccaggccag accgaggtcg tgcgtgatgg ccaggatgga gatgccggtc
     1921 tcctggacca ggttcagcat gagggcgagg atctcgcccc ggacggaggc gtcgagggcc
     1981 gacaccggct cgtcggccac gatc
//
LOCUS       DASTFV010000483         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1787099, whole genome shotgun sequence.
ACCESSION   DASTFV010000483 DASTFV010000000
VERSION     DASTFV010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787099"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFP02_08850"
     CDS             complement(<1..188)
                     /locus_tag="VFP02_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543176.1"
                     /translation="MSNAKQQLIEHLLEFSVRRGDFTLKSGKQSNWFLDAKQTCCRAE
                     GILLVADLALEAIPDEVTA"
     gene            complement(185..1168)
                     /gene="purM"
                     /locus_tag="VFP02_08855"
     CDS             complement(185..1168)
                     /gene="purM"
                     /locus_tag="VFP02_08855"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET9543177.1"
                     /translation="MGESYATAGVDIDAGEEAVRRIGPAVRSTFTESVLTDVGGFGAP
                     FALDTSRFTQPVLVSSTDGVGTKLLVAEATGRYDTIGIDLVAMVVDDLVCQGAEPLFL
                     LDYLAVGALDPDVVEQVVNGIAEGCRQARCALIGGEMAEHPGSMAPDRIDVAGFAVGV
                     VERDRLITGDQARPGDLLIGLPSPGLRSNGYSLARRVLLEVAGLPLDDLADDLLLPSV
                     IYAPAVVDLLAEVDVRGVAHITGGGIPGNLPRALPDDVGYWLDRATWEVPAIFGLIQE
                     HGQVSDDEMAKVFNLGIGMVVVVTPADAERTIAALAASGHAARAIGEVTKG"
     gene            complement(1176..>2238)
                     /locus_tag="VFP02_08860"
     CDS             complement(1176..>2238)
                     /locus_tag="VFP02_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET9543178.1"
                     /translation="RSDGRGLESALFATLPKFEGAFSLVVMDQGRIIGVRDPNGFRPL
                     CLGKLEDGGWVLASESPALDIVGAHFVRELLPGEMVVIDAAGPRSIKPFPDERVDPKL
                     CLFEFVYFARPDTRLYGQSVHAARVKMGGQLAEQAPVEADLVMGVPESGLPAAEGYSH
                     RSGIPYGQGLVKNRYIGRTFIAPSQEMRALGVRMKLNPLRDAIAGQRLIVVDDSIVRG
                     TTTRAMVKMLREAGATEVHLRVSSPPYRWPCFYGMDTGTRGELLAANLTVAEIQEYLG
                     VDTLAYLELDRLVDATGAVGAGFCNACLTGDYPVAIPENLHKGVLEHGDEVSETRVAA
                     PRLLDAEDARLPADVARED"
BASE COUNT          331 a          775 c          825 g          307 t
ORIGIN      
        1 gccgtcacct cgtcggggat cgcctcgagg gccagatcgg ccacgaggag gatcccctcg
       61 gcccgacagc aggtctgttt ggcgtccagg aaccagttgc tctgcttgcc cgacttcagc
      121 gtgaagtccc cccgccgcac ggagaactcg agcaggtgct cgatgagctg ctgcttggcg
      181 ttgctcaccc cttcgtgacc tcgccgatgg cgcgggcggc gtggcccgac gcggccagcg
      241 cggcgatcgt gcgctccgcg tcggccggcg tgacgacgac caccatgccg atgccaaggt
      301 tgaagacctt ggccatctcg tcgtcgctga cctggccgtg ctcctggatg agaccgaaga
      361 tcgccggcac ctcccacgtg gcgcgatcca gccagtagcc gacgtcgtcg ggcagcgccc
      421 gggggaggtt gccggggatg cccccaccgg tgatgtgggc cacaccccgc acgtcgacct
      481 cggccagcag gtcgaccacg gccggggcgt agatgaccga aggcaggagc agatcgtcgg
      541 cgaggtcgtc gagggggagt ccggccactt ccagcaggac ccgtcgggcc agggagtacc
      601 cgttggagcg cagcccggga gagggcaacc cgatgaggag gtcgccgggg cgagcctggt
      661 cgccggtgat cagccggtcc cgttcgacca cgccgacggc gaagccggcc acgtcgatgc
      721 ggtcgggcgc catggacccg gggtgttccg ccatctcgcc cccgatgagg gcacagcggg
      781 cctggcggca gccctcggcg atgccgttga cgacctgctc gaccacatcg gggtcgagcg
      841 cgccgacggc gaggtagtcc agcaggaaga ggggctcggc cccctggcag accaggtcgt
      901 cgacgaccat ggccaccagg tcgatgccga tggtgtcgta gcgcccggtg gcctcggcca
      961 ccagcagctt ggtgcccacc ccgtcggtcg aggacaccag caccggctgg gtgaagcggg
     1021 aggtgtcgag ggcgaagggg gccccgaagc cgccgacgtc ggtcaggacc gactcggtga
     1081 aggtggagcg cacggcgggg ccgatgcggc gaaccgcctc ctcgcccgca tcgatgtcca
     1141 cgccggcggt ggcgtagctc tcccccaaga gggacctagt cctcgcgggc gacgtcggcg
     1201 gggaggcggg cgtcctcggc gtcgagcagg cggggggcgg cgacccgggt ctcgctgacc
     1261 tcgtcgccgt gctccagcac gcccttgtgc aggttctcgg ggatggccac cgggtagtcg
     1321 ccggtcaggc aggcgttgca gaagccggcc ccgaccgcgc cggtcgcgtc gaccaggcgg
     1381 tccagctcca ggtaggccag cgtgtcgacg ccgaggtact cctggatctc cgccactgtc
     1441 aggttggcgg ccagcagctc gccccgggtc ccggtgtcca tgccgtagaa gcagggccag
     1501 cggtaggggg gcgaggacac ccgcaggtgg acctcggtgg ctcccgcctc ccgcagcatc
     1561 ttgaccatgg cccgggtggt ggtgccccgc acgatggagt cgtcgaccac gatcaggcgc
     1621 tggccggcga tggcgtcccg cagggggttc agcttcatgc gcacgccgag cgcccgcatc
     1681 tcctggctgg gggcgatgaa cgtccgcccg atgtagcggt tcttgaccag gccctggccg
     1741 tacgggatcc cgctgcggtg cgagtagccc tcggccgcgg gcaggcccga ctcgggcaca
     1801 cccatgacca ggtcggcctc gaccggcgcc tgctcggcca gctggccacc catcttgacc
     1861 cgggccgcgt gcacgctctg gccgtagagg cgggtgtccg ggcgggcgaa gtagacgaac
     1921 tcgaagaggc agagcttggg atcgacgcgc tcgtcgggga agggcttgat cgaccgcggg
     1981 ccagcggcgt cgatcaccac catctcgccc ggcagcagct cgcggacgaa gtgggcgccg
     2041 acgatgtcga gcgccgggct ctccgacgcc aggacccagc cgccgtcctc gagcttgccc
     2101 aggcagagcg ggcggaaccc gttggggtcg cgaaccccga tgatccggcc ctgatccatc
     2161 accaccagcg agaaggcgcc ttcgaacttg gggagggtgg cgaatagggc gctctccagc
     2221 ccccggccat cggagcgc
//
LOCUS       DASTFV010000484         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1787349, whole genome shotgun sequence.
ACCESSION   DASTFV010000484 DASTFV010000000
VERSION     DASTFV010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787349"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VFP02_08865"
     CDS             <1..944
                     /locus_tag="VFP02_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET9543179.1"
                     /translation="PLVLPRVTPDPEAHARPVDKVVPAHHAVEGAGFEVWRPFPGNVD
                     AHVADPFFLLDQLGPVDYAPNEPVGAPWHPHRGFETVTYVIDGVVEHHDSNGGGGIIG
                     EGDTQWMTAGAGILHDEVPTEIFTRNGGRSHGVQLWVNLPAALKMSEPRYQAITSHDL
                     TLLSSADGGALIRLIAGDLDGHAGPGATHTPITYAHASVRPGAQVTVPWNPAFSAMAY
                     VLTGRGHAGDERRPVDEHQLVLFGPGDTLIIGAADDQPEASPDLEVLLLGGLPIGEPI
                     AHYGPFLMNTRDELRQAVEDFNAGRMGQIPATSLRPN"
     gene            complement(911..1984)
                     /locus_tag="VFP02_08870"
     CDS             complement(911..1984)
                     /locus_tag="VFP02_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543180.1"
                     /translation="MSRPTSWRSALAPVGLAAVAGLLSTSCGSEAPAAADSPDPVFAL
                     DRDVTDGIEVPDPDRTDVAPAPDLRILLQTLLAEHSELAIQTMRAVIDETPDLDALRE
                     AITVNTDALTEAIGLVYGPSVARAFDQLWTQHIEFFAAYAGALGDGDDDAAAEARDQL
                     EDYALDFSSFCSTATGGAAPVALVEGLLHDHVRQLLGQADEWAGGDRGAAYASQAEAH
                     AHAAAIAAVLAGAISAQQPDAFPGDSASDLIEALAERRTVVTAYAIGQMAVAKAEILD
                     VGDVSQAQALAEDTTEAMTTLAPAPDPDALAHLVAEYRAALENRDFTAVEAARVELDT
                     LAHELAVGRPLQGQLGRRLVAGI"
BASE COUNT          283 a          728 c          746 g          275 t
ORIGIN      
        1 accccctggt cctcccgcgg gtcacccctg accccgaggc ccacgcccga cccgtcgaca
       61 aggtcgtgcc cgcccaccac gcggtggagg gcgccggctt cgaggtctgg cgaccgttcc
      121 cgggcaacgt cgacgcccac gtggccgacc ccttcttcct gctcgaccag ctcgggccgg
      181 tcgactacgc ccccaacgag ccggtcggcg ccccgtggca cccgcaccgg ggcttcgaga
      241 ccgtcaccta cgtgatcgac ggggtcgtcg agcaccatga ctccaacggt gggggcggga
      301 tcatcggtga aggtgacacc cagtggatga cggccggcgc cgggatcctg cacgacgagg
      361 tcccgacgga gatcttcacg cgcaacggtg gccgctccca cggcgtccag ctctgggtga
      421 acctgccggc cgccctcaag atgagcgagc cccgctacca ggccatcacc agccacgacc
      481 tgacgctgct gtcctcggcc gacggtgggg ctctcatccg cctcatcgcg ggggacctcg
      541 acggacacgc cggtcccggg gcgacgcaca cgcccatcac ctacgcccat gccagcgtcc
      601 ggcccggggc gcaggtcacc gtgccctgga accccgcctt cagcgccatg gcctacgtgc
      661 tcaccggtcg gggccacgcc ggtgacgagc gccgacccgt cgacgagcac cagctcgtgc
      721 tgttcggccc cggtgacacg ctgatcatcg gcgccgccga cgatcagccc gaagcgtcac
      781 ccgatctcga ggtcctcctc ctggggggac tgccgatcgg cgagcccatc gcccactacg
      841 gcccgttcct gatgaacacg cgggacgagc tgcgacaggc cgtcgaggac ttcaacgccg
      901 gccgcatggg tcagatcccg gccaccagcc tccgccccaa ctgaccctgg aggggtcggc
      961 cgacggcgag ctcgtgggcc agcgtgtcga gctcgacacg ggcggcctcg acggcggtga
     1021 agtcgcggtt ttcgagggca gcgcggtact cggccaccag gtgggcgaga gcgtcggggt
     1081 cgggcgcagg ggcgagggtg gtcatcgcct cggtggtgtc ctcggccagg gcctgggcct
     1141 gggagacgtc gccgacgtcc aggatctcgg ccttggccac ggccatctgc ccgatggcgt
     1201 aggcggtgac gacggtgcga cgctcggcca gcgcctcgat caggtcggag gcggaatcgc
     1261 cggggaaggc gtcgggttgc tgggcgctga tcgctccggc cagtaccgcg gcgatggccg
     1321 cggcgtgcgc gtgagcctca gcctggctgg cgtacgccgc gccccggtcg ccgccggccc
     1381 actcgtcggc ctggcccagc agttggcgga cgtggtcatg gagcaggccc tccaccaacg
     1441 ccaccggcgc cgcgccaccg gttgcggtcg agcagaagga ggagaagtcg agcgcgtagt
     1501 cctcgagctg gtcgcgggct tcggccgccg cgtcgtcgtc gccgtccccg agcgcaccgg
     1561 cgtacgcggc gaagaactcg atgtgctggg tccagagctg gtcgaaggcc cgggccaccg
     1621 aaggtccgta gaccaggccg atggcctcgg tcagggcgtc ggtgttgacc gtgatggcct
     1681 cgcgcagggc gtcgaggtcc ggcgtctcgt cgatgacggc gcgcatggtc tggatggcca
     1741 gctccgagtg ctcggccagg agggtctgca gcaggatgcg cagatcgggg gcgggcgcca
     1801 cgtcggtccg gtccggatcc ggcacctcga tgccgtcggt gacgtcgcgg tccagggcga
     1861 agacgggatc cggggagtcg gcggcggccg gcgcttcgga gccgcagctg gtcgagagca
     1921 gtccggcgac ggccgccagg ccgaccgggg ccagcgccga cctccaggag gtgggccggc
     1981 tcatgccggg gccgcctcgt gaggaagggt cagggtctgc gggtcgaagc cg
//
LOCUS       DASTFV010000485         3220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1790057, whole genome shotgun sequence.
ACCESSION   DASTFV010000485 DASTFV010000000
VERSION     DASTFV010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790057"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VFP02_08875"
     CDS             complement(<1..1022)
                     /locus_tag="VFP02_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543181.1"
                     /translation="MSTVSSALRRAPHQLMFWLVIAVFAVGALRAFFPIEGFDEVRTG
                     EVPLEVAGSDDGVWVLNYADHTVSLVRTSDQEVLLTTEVGADVAPALSANDDGAWLIL
                     DAGATIGRVDASGEIVDRFDVSDTVDGIAQDLAAGPDFVWVTTGEGQQMVRIDAGTGE
                     IGDVIDVGEVVVQPQVLGDSLWVFETDGITEYDNTTGEELNKIDTSDIRVHDFYATGD
                     EVYVLANVDNFEETGLVVRLDPETNRENGRYRLTETKPSHITVADGQVFVTGSGGLLM
                     ELTTDPDRPPTLIATEQVTVSTKDLRGVTVLDDIVWVADGTNGIVHQPVTGVEGGDIT
                     DVTEPQV"
     gene            complement(1055..1882)
                     /locus_tag="VFP02_08880"
     CDS             complement(1055..1882)
                     /locus_tag="VFP02_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017128 - phospholipid scramblase activity
                     [Evidence IEA];
                     GO_process: GO:0017121 - plasma membrane phospholipid
                     scrambling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid scramblase-related protein"
                     /protein_id="HET9543182.1"
                     /translation="MTEAQTPAANWYPDPGGKHEHRYWDGSQWTDNVADHGRQAVDPL
                     VANHVPTTHHSAQNVQQQVQQRAGLQATAAGGGTLFTEPVLVVNQKRKLIELNTEFAV
                     FDQHGTQIGAVRQVGQSSAKKALRFVANVDQFLTHKFQIVDIQGNVVLALTRPAKLLK
                     SKVLITDGLGQPVGDVVQQNAIGKIRFALEAGGQTLGSINAENWRAWNFAIQDHTGAE
                     VARITKTWEGLATTLFTTADNFVVQIHRQLEEPLRSLVVASALCVDVALKQDDRGFN"
     gene            complement(1902..>3220)
                     /locus_tag="VFP02_08885"
     CDS             complement(1902..>3220)
                     /locus_tag="VFP02_08885"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9543183.1"
                     /translation="IAFGAVFDREAGGELALAREGGHSEPRIVHAGGAATGAEVERAL
                     VAAARATAAEVYEHTFAIDLVVEGGRCRGVRAYGRDGHRLEIRARHTLLATGGAGQVF
                     AVTTNPTEATGDGIAMAHRAGAVLADVEFIQFHPTALHHPKMPKPLLSEALRGHGALL
                     RDLDGNRFVDELLPRDQVSRAMTAKMIEQDVDHMWLDATVLENFDERFPTIAASLEEV
                     GLDPATDWLPIAPAAHYTCGGMVTDLDGASSLPGLWGAGEVTCTGVHGANRLASNSLL
                     EGMVFGPRVVEAIERGQDGPEATGAMRCVHGTDAPGVIPGLPVTSGGVSLRREVVPHQ
                     AVGEPDAPTTAPEWRALLQKAMTLGAGVRRDADSLRQAARALGRIGRAAEGQDAIDWY
                     EVRNLVDVAAMIVAAATARTETRGAHTREDFPETDPALRARFLHRT"
BASE COUNT          441 a         1060 c         1194 g          525 t
ORIGIN      
        1 acctgaggct cggtgacgtc ggtgatgtcg ccgccctcga ccccggtgac gggctggtgg
       61 acgatgccgt tggtgccgtc ggccacccag acgatgtcgt cgagaacggt cacgccccgc
      121 aggtccttcg tcgacaccgt cacctgctcg gtggcgatga gcgtcggtgg gcggtcggga
      181 tcggtggtca gctccatgag caggccaccg ctgcccgtga cgaagacctg gccgtcggcc
      241 acggtgatgt gcgaaggctt cgtctcggtg aggcggtacc gaccgttctc cctgttcgtc
      301 tcgggatcga ggcggacgac cagcccggtc tcctcgaagt tgtccacgtt ggcgaggacg
      361 tagacctcgt ccccggtggc gtagaagtcg tgcacccgga tgtcggacgt gtcgatcttg
      421 ttgagctctt cacccgtcgt gttgtcgtac tcggtgatgc cgtcggtctc gaagacccag
      481 agcgagtcac cgaggacctg tggctgcacg acgacctcgc cgacatcgat gacgtcgccg
      541 atctcgccgg tgccggcgtc gatgcggacc atctgctggc cctcgccggt ggtcacccag
      601 acgaagtcgg ggccggccgc caggtcctgg gcgatcccgt cgacggtgtc cgacacgtcg
      661 aagcggtcca cgatctcccc gctcgcgtcg acccggccga tcgtggcgcc ggcgtcgagg
      721 atgagccagg ccccgtcgtc gttcgcgctg agcgcagggg cgacgtcggc cccgacctcg
      781 gtggtcagca ggacctcctg gtcggaggtg cgcacgaggc tgacggtgtg gtcggcgtag
      841 ttgaggaccc agaccccgtc gtcgctgccg gcgacctcga gggggacctc gccggtgcgg
      901 acctcgtcga agccctcgat ggggaagaag gcgcgcaggg caccgacggc gaagacggcg
      961 atgaccagcc agaacatgag ttggtgcggg gcgcggcgga gagccgaaga cacggtggac
     1021 atgagatggg gaattccttc aggggggagg ggcgtcagtt gaagccgcgg tcgtcctgct
     1081 tcagggcgac gtcaacgcac agcgcgctgg cgacgacgag ggagcgaagt ggctcctcga
     1141 gctgacggtg gatctggacg acgaagttgt cggcggtcgt gaagagggtc gtcgccaggc
     1201 cctcccacgt cttggtgata cgggccactt cggcgcccgt gtggtcttgg atggcgaagt
     1261 tccacgcccg ccagttctcg gcgttgatgg aaccgagggt ctggcccccg gcctccaggg
     1321 cgaagcggat cttgccgatc gcgttctgct gcacgacgtc gccgacgggt tggcccaggc
     1381 cgtcggtgat cagcaccttc gacttgagga gcttggccgg gcgggtgagg gccagcacga
     1441 cgttgccctg gatgtcgacg atctggaact tgtgggtcag gaactggtcg acgttggcca
     1501 cgaagcgcag ggccttcttg gccgagctct ggccgacctg gcggacggcg ccgatctggg
     1561 tgccgtgctg gtcgaagacg gcgaactcgg tgttgagctc gatgagcttg cgcttctggt
     1621 tcaccacgag gacgggctcg gtgaacaacg tgcccccgcc ggcggcggtg gcctggaggc
     1681 cggcccgctg ctggacctgc tgctggacgt tctgggcgga gtggtgggtg gtggggacgt
     1741 ggttggccac cagggggtcg acggcctgcc gaccgtggtc agccacgttg tcggtccact
     1801 gggagccgtc ccagtaacgg tgctcgtgct tcccgcccgg atccggatac cagttggcgg
     1861 cgggcgtctg cgcctcggtc atcgttgcga aagcgtaacg gtcatgttct gtggaggaac
     1921 ctcgcgcgga gcgcggggtc cgtctcgggg aagtcttctc gggtgtgggc gccgcgggtc
     1981 tccgtccggg cggtggcggc cgccacgatc atggcggcca catcgaccag gttgcgtacc
     2041 tcgtaccagt cgatcgcgtc ctgaccttcg gcggcccggc cgatccgtcc gagcgctcga
     2101 gccgcctgac gcagggagtc ggcgtcacga cggacgccgg cgccgagcgt catcgccttc
     2161 tgcaggaggg cccgccactc gggggcggtg gtcggcgcgt cgggctctcc gaccgcctgg
     2221 tgcgggacca cctctcggcg caagctgact cccccgctcg tcaccggcag gccggggatg
     2281 acgccggggg cgtcggtgcc gtggacgcag cgcatggcgc ccgtggcctc gggcccgtcc
     2341 tgaccgcgct cgatggcctc gaccaccctg ggcccgaaga ccatgccctc cagcagggag
     2401 ttggaggcca ggcggttggc gccgtgaacg ccggtgcacg tgacctctcc ggcgccccac
     2461 aggccgggca gggacgaggc gccgtcgagg tcggtcacca tcccgccgca cgtgtagtgg
     2521 gccgcggggg cgatgggcag ccagtccgtc gccgggtcga ggcccacctc ctccaacgag
     2581 gcggcaatgg tcgggaagcg ctcgtcgaag ttctccagga cggtggcgtc cagccacatg
     2641 tggtccacgt cctgctcgat catcttcgcc gtcatggccc gcgagacctg gtcccggggc
     2701 agcagctcgt cgacgaagcg gttgccgtcc aagtcccgca acaacgcccc gtggccccgt
     2761 agggcttcgg acagcagggg cttgggcatc ttggggtggt gcagcgccgt cgggtggaac
     2821 tggatgaact cgacgtcggc caggaccgcc cccgcacgat gggccatggc gatgccatct
     2881 cccgtggctt cggtcgggtt ggtggtgacg gcgaagacct ggccggcgcc ccccgtggcc
     2941 agcagggtgt gcctggcccg gatctcgagc cggtggccgt ccctgccgta ggcccgcacc
     3001 ccccggcagc gtccaccctc gaccacgagg tcgatggcaa aggtgtgctc gtacacctcg
     3061 gccgcggtgg cgcgggcggc ggcgaccagg gcccgctcga cctcggcccc ggtggctgcg
     3121 cctcccgcgt ggacgatgcg gggctcggaa tggccgccct cccgggccag ggccagctcg
     3181 ccgccggcct cccggtcgaa gacagcaccg aaggcgatca
//
LOCUS       DASTFV010000486         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1797879, whole genome shotgun sequence.
ACCESSION   DASTFV010000486 DASTFV010000000
VERSION     DASTFV010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797879"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /gene="infB"
                     /locus_tag="VFP02_08890"
     CDS             <1..1503
                     /gene="infB"
                     /locus_tag="VFP02_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET9543184.1"
                     /translation="PPVVTVMGHVDHGKTKLLDAIRNTDVVAREAGGITQHIGAYQVR
                     AGADGRLVTFIDTPGHEAFTAMRARGAAVTDVAVLVVAADDGVMPQTVEAINHAKAAG
                     VPIVVAVNKVDKEDANPAKVRQQLTEYDLVAEEYGGKTQFVDVSAKTQQNLDTLVETI
                     LLVADVELNLKANPERRAQGSVIEAHLDKGRGPVATILVRRGTLHVGDAVAAGTAFGR
                     VRAMFDENGKEVTAAVPAQPVVVIGLSAPPSAGDEVRAVTDERIARGISQTREARQRQ
                     AAIATSRAPRLEDLFARIREGQLQELPLIIKADVMGSVEAIDDALGRIEIPEVRLRVL
                     HKAAGAITEDDVSLAAVAGAIIIGFSVRPTPAARELANREGVDIRLYSVIYQVVEDIE
                     KALKGMLAPEYEERALGTAEVRQTFRVPRIGVVAGSYVTSGEVARGAKARLVRDGVVV
                     YDGRIGSLRRFKDDVRTVAAGYECGIGLDNFQDVKEGDQIEAYEMVEIPR"
     gene            1574..1864
                     /locus_tag="VFP02_08895"
     CDS             1574..1864
                     /locus_tag="VFP02_08895"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HET9543185.1"
                     /translation="MFVALLRLDLRLPDASSLKDKRSVVKSLAAALRKLNCTVAEVDH
                     QDLRQRATLAVATVAGEGFHARRVLVAAEREVERTPGVELLASDVTMYGPED"
     gene            1871..>2223
                     /gene="rbfA"
                     /locus_tag="VFP02_08900"
     CDS             1871..>2223
                     /gene="rbfA"
                     /locus_tag="VFP02_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET9543186.1"
                     /translation="MAKPSYPRARRLAETIRRLAGEWLEAEQADQPVPLMTVTDVRVT
                     EDLHHASVYFTVLGDDQQRAAAEAWLAESATGARTFVASQLRLRHAPTLEFVPDTVPG
                     QGARIDRLLAELGAP"
BASE COUNT          343 a          812 c          793 g          275 t
ORIGIN      
        1 ccgccggtcg tgaccgtcat gggccacgtc gaccacggca agaccaagct gctggacgcc
       61 atccgcaaca ccgacgtggt cgcccgcgag gccggcggca tcacccagca catcggcgcc
      121 taccaggtca gggcgggtgc cgacggccgg ctggtgacct tcatcgacac cccgggccac
      181 gaggcgttca ccgccatgcg ggccaggggc gcggccgtca ccgacgtcgc cgtgctggtg
      241 gtcgccgccg acgacggggt catgccccag accgtggagg cgatcaacca cgccaaggcg
      301 gccggggtgc cgatcgtggt ggcggtcaac aaggtcgaca aggaggacgc caacccggcc
      361 aaggtccgcc agcagctgac cgagtacgac ctggtggccg aggagtacgg cggcaagacc
      421 cagttcgtcg acgtgtcggc caagacccag cagaacctcg acaccctggt cgagaccatc
      481 ctgctggtcg ccgatgtcga gctgaacctc aaggccaacc ccgagcgccg tgcccagggc
      541 tcggtcatcg aggcccacct ggacaagggc cgcggcccgg tggcgaccat cctggtccgc
      601 cggggcaccc tgcacgtcgg ggacgcggtg gccgccggca ccgccttcgg gcgggtccgg
      661 gccatgttcg acgagaacgg caaggaggtc accgccgccg tcccggccca gccggtcgtg
      721 gtcatcggcc tctcggctcc gcccagcgcg ggcgacgagg tcagggcggt caccgacgag
      781 cgcatcgccc gcgggatctc ccagacccgc gaggcgcgcc agcgccaggc ggcgatcgcg
      841 accagccggg ccccgcgcct ggaggacctg ttcgccagga tccgcgaggg ccagctccag
      901 gagctgccgc tgatcatcaa ggccgacgtg atgggctcgg tcgaggccat cgacgacgcc
      961 ctcgggcgga tcgagatccc cgaggtccgg ctgcgggtgc tccacaaggc ggccggcgcc
     1021 atcaccgagg acgacgtcag cctggccgcc gtggccgggg ccatcatcat cggcttctcg
     1081 gtgcggccga ccccggccgc gcgcgagctg gccaaccgcg aaggcgtcga catccgcctg
     1141 tactcggtca tctaccaggt ggtggaggac atcgagaagg ccctcaaggg catgctcgcc
     1201 cccgagtacg aggagcgcgc cctcggcacc gccgaggtca ggcagacctt ccgggtgccg
     1261 cggatcggcg tcgtcgccgg gtcgtacgtc acctcgggcg aggtcgcccg cggggccaag
     1321 gcccgcctgg tccgcgacgg cgtggtcgtc tacgacgggc ggatcggatc gctgcgtcgc
     1381 ttcaaggacg acgtccgtac ggtcgcggcc ggctacgagt gcggcatcgg cctggacaac
     1441 ttccaggacg tcaaggaagg cgatcagatc gaggcctacg agatggtcga gatcccacgg
     1501 taaccccgaa ggccccgggg ccacggcgca cgcctcggcg tttgccgcca ggtcccgggg
     1561 cgaccctgct gccatgttcg tcgccctgct ccgcctggac ctccggctcc cggacgcctc
     1621 gtcgctcaag gacaagcggt cggtcgtcaa gagcctggcc gccgcgttgc gcaagctcaa
     1681 ctgcacggtc gccgaggtcg atcatcagga cctgcgccag cgggccaccc tggcggtggc
     1741 cacggtggcc ggcgagggct tccacgcccg ccgggtgctg gtggcggccg agcgcgaggt
     1801 cgagcgcacg cccggcgtcg agctgctggc cagcgacgtg accatgtacg gacccgagga
     1861 ctgaaccgcg atggcaaagc cgagctaccc cagagcccgc cgcctggccg agacgatccg
     1921 gcggctggcc ggcgaatggc tggaggccga gcaggccgac cagccggtcc cgctgatgac
     1981 cgtgaccgac gtgcgcgtca ccgaggacct gcaccacgcc agcgtctact tcaccgtgct
     2041 cggcgacgac cagcagcggg ccgccgccga ggcctggctg gccgagtcgg ccaccggggc
     2101 acggaccttc gtggcctcgc agctgcggct ccgtcacgca ccgacgctag aattcgttcc
     2161 cgacaccgtg cccggccagg gtgcgcggat cgaccggttg ctggccgagc tgggcgcgcc
     2221 gga
//
LOCUS       DASTFV010000487         2770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1799531, whole genome shotgun sequence.
ACCESSION   DASTFV010000487 DASTFV010000000
VERSION     DASTFV010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799531"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..692)
                     /locus_tag="VFP02_08905"
     CDS             complement(192..692)
                     /locus_tag="VFP02_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET9543187.1"
                     /translation="MDRWWAGWRHAYIDAAFGEEEPEGEGSLFERILGSGMPDEETYV
                     VARGPVVSALLNAYPYNSGHLLVMPNRAVPGLDDLTEDEETALWRTVRHGVTAITAAY
                     APEGVNVGLNLGRAAGAGVPDHLHVHCLPRWSGDTNFMTAVAETRVLPEPLDVTWRRL
                     RDAWPV"
     gene            complement(695..2638)
                     /gene="thrS"
                     /locus_tag="VFP02_08910"
     CDS             complement(695..2638)
                     /gene="thrS"
                     /locus_tag="VFP02_08910"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET9543188.1"
                     /translation="MAIDVTLPDGSSRSLDEGATAGDLAAAIGRGLAKAAVVAEINGE
                     ERELTTPLHDGDAVAIVTDTTDEGRYVIRHSTAHVLAQAVLDLFEGATFGIGPPIEDG
                     FYYDFQLADGATFTPDDLDRIDARMREIIKEKQPFLRDEIPDAEAKELFAAHPFKLEI
                     IEGTADDPMSATPGGLVRTYENPPDFIDLCRGPHVPDTGRLGHFKLTRVAGAYWRGDE
                     RNPMLQRIYGTAWESKQALEDHLERLEQARERDHRKLATELDLLSFPDELGGGLAVWH
                     PKGGIVRKLMEDYSRQRHQDGGYEFVYSPNLARSTLWETSGHLDWFADAMYPPMETEN
                     GAYYPKPMNCPFHMLIFGSRQRSYRELPLRLFELGTVYRYERSGVLHGLMRIRGFTQD
                     DSHIFCTRDQIQDELVSLLDFVISVLEAFGFDDFTASLSTKPEGKAVGSDEEWDEATE
                     ALRRALEVRELEYEVDVGGGAFYGPKIDIHVRDAIGRQWQLSTLQVDFQLPERFGLDY
                     IGPDNHPHRVSVIHRALFGSVERFFGVMLEHYAGAFPAWLAPVQARVLGVRDDHERHA
                     AKVVEKLQAAGFRADTMDADEPLGSRIRKAKLEKLPYILVVGDDDVAEGTVGVNPRGG
                     EVERGVSVEDFIERLAAEVESHS"
BASE COUNT          376 a          964 c          997 g          433 t
ORIGIN      
        1 ctcgcagagc aggccgaggc cgatgtagat gtagcccggt atccgacggc gcttgttgtc
       61 cgggaaggtg tagggcccga cgaaggccga cgcgtccagg tcggcgggga gctcgtcgcg
      121 gtgctcgccg ggctgctcgt cgaccggctc ctcctcgtcg ctcacggccg ccgacggtac
      181 ggcacgggcg ctcaaaccgg ccaggcgtcg cggaggcggc gccaggtgac gtcgagcggc
      241 tcgggcagga cccgggtctc ggccacggcg gtcatgaagt tggtgtcgcc cgaccagcgg
      301 ggcaggcagt ggacgtggag gtggtccggg accccggccc ctgcagcccg ccccaggttc
      361 aggcctacgt tcaccccttc gggcgcatag gccgcggtga tggcggtgac cccgtggcgg
      421 acggtgcgcc agagggcggt ctcctcgtcc tcggtcaggt cgtccaggcc gggcaccgcc
      481 cgattcggca tcaccaggag gtgacccgag ttgtaggggt aggcgttgag gagcgcggac
      541 accacagggc cccgggccac gacgtaggtc tcctcgtcgg gcatcccgct gcccaggatg
      601 cgctcgaaga gcgagccctc cccctcgggt tcctcctcgc cgaaggcggc atcgatgtac
      661 gcgtgccgcc agccggccca ccaccggtcc atggtcacga gtggctctcg acctccgcgg
      721 ccaggcgctc gatgaagtcc tcgacgctga cgccccgctc gacctcgcca ccgcgggggt
      781 tcaccccgac ggtcccctcg gccacgtcgt cgtcgccgac caccaggatg tacggcagct
      841 tctccagctt ggccttccgg atgcgggacc ccagcggctc gtcggcgtcc atcgtgtcgg
      901 cccggaaccc ggcggcctgc agcttctcga ccaccttggc ggcgtggcgc tcgtggtcgt
      961 cacggacgcc gaggacccgg gcctgcaccg gcgccagcca ggccgggaac gccccggcgt
     1021 agtgctcgag catcaccccg aagaagcgct ccaccgaccc gaacagggcg cggtggatga
     1081 cgctcacccg gtgggggtgg ttgtcggggc cgatgtagtc gaggccgaag cgctcgggca
     1141 gttggaagtc gacctgcagc gtcgacagct gccactggcg accgatggcg tcccggacgt
     1201 ggatgtcgat cttgggcccg tagaacgcgc cgccgccgac atcgacctcg tactccagct
     1261 cccgcacctc gagagcccgg cgcagggcct cggtggcctc gtcccattcc tcgtcgctgc
     1321 cgacggcctt gccctcgggc ttggtcgaga ggctggccgt gaagtcgtcg aagccgaagg
     1381 cctccagcac cgagatcacg aagtcgagga ggctgaccag ctcgtcctgg atctggtcgc
     1441 gggtgcagaa gatgtggctg tcgtcctggg tgaagccgcg gatgcgcatg aggccgtgca
     1501 ggacccccga gcgctcgtag cggtagaccg tgcccagctc gaacaggcgg agcggcagct
     1561 cccggtagct ccgctgccgg ctgccgaaga tcagcatgtg gaacgggcag ttcatcggct
     1621 tcgggtagta cgccccgttc tccgtctcca tgggcgggta catggcgtcg gcgaaccagt
     1681 ccaggtgccc gctggtctcc cacagcgtcg accgggccag gttgggcgag tacacgaact
     1741 cgtagccccc gtcctggtgg cgctgacggc tgtagtcctc catcagcttg cggacgatgc
     1801 cccccttggg atgccagacg gcgaggccgc cgcccagctc gtcggggaag gacaggaggt
     1861 cgagctcggt ggccagcttg cggtggtccc gctcccgcgc ctgctcgagg cgctcgaggt
     1921 ggtcctccag ggcctgcttg gactcccacg ccgtgccgta gatgcgctgc agcatcgggt
     1981 tgcgctcgtc gccccgccag taggcaccgg ccacccgcgt cagcttgaaa tgacccagac
     2041 gacccgtgtc cgggacgtgg ggcccccggc agaggtcgat gaagtcgggc gggttctcgt
     2101 acgtgcggac caggccaccc ggcgtggccg acatcgggtc gtcggccgtc ccttcgatga
     2161 tctccagctt gaacgggtgg gcggcgaaga gctccttggc ctcggcgtcg gggatctcgt
     2221 cgcgcaggaa gggctgcttc tccttgatga tctcccgcat gcgggcgtcg atgcggtcga
     2281 ggtcgtcggg cgtgaacgtg gccccgtcgg ccagctggaa gtcgtagtag aagccgtcct
     2341 cgatcggcgg gccgatgccg aacgtggccc cctcgaacag gtcgagcacg gcctgggcca
     2401 gcacgtgggc cgtcgagtgc cggatgacgt agcgaccctc gtccgtcgtg tccgtgacga
     2461 tggcgaccgc gtctccgtcg tgcagcggcg tggtcagctc ccgctcttcg ccgttgatct
     2521 cggcgacgac cgcggccttg gccaggcccc gcccgatggc ggcggccagg tcgccggcgg
     2581 tggcgccctc gtcgagggag cgcgaggaac cgtccgggag cgttacgtca atggccatgg
     2641 agggccgagg ataccgacgg ccctcctccg gctagccggc tttcgccacc tcggccaggg
     2701 cggcgtgccc gttggcctgg ggcccgtcgg cggtgatgac gccgccccgc cggcgcggga
     2761 tcgcgtcctc
//
LOCUS       DASTFV010000488         2908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1801010, whole genome shotgun sequence.
ACCESSION   DASTFV010000488 DASTFV010000000
VERSION     DASTFV010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1801010"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1501
                     /locus_tag="VFP02_08915"
     CDS             98..1501
                     /locus_tag="VFP02_08915"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9543189.1"
                     /translation="MGTRRGGRSVVLVMAVFALVAGCSDDTGGDHAASDEDPTVTVVS
                     QNLLHGTACAEDSDRCDLPARVQLFVDQLADAGCPELVSVQESNQQTVDELASALGDG
                     CTAEYAIVWDDDPTLDRETVLSTEPVLGSRRIALAGPLRSAYWVRVASDVGIVDYISS
                     HLASSSDDRPCDEATCPPPCEVADTVNTCQGRQLVEFADEVADPEAVVVIGGDLNAMP
                     DEPTIAAITGAGYVDTHLAVGNPECDPDTGQQCTSGRIDDALTDLEDPASLQSERIDY
                     LFVGGPRDCTPVEPTGLFNGEPADGTLAFPSDHTGVQTTLSCPTNPEQLDAAPSATVP
                     AVTTTTTGPAAPVDAETEAAITQAFTNVFDGSVTDVGVKLASLESADELEPYFLESLE
                     ATQDIASRIRLQIDAIEAVDARTADVTYTLLLDGAAVLDHLPGQAVKVDGEWLVSLRT
                     YCDVSTQGADTIPEPCQ"
     gene            complement(1506..>2908)
                     /locus_tag="VFP02_08920"
     CDS             complement(1506..>2908)
                     /locus_tag="VFP02_08920"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HET9543190.1"
                     /translation="LHNAAAVCVRGEVVGVYRKQHLPNYAVFDEQRYFEPGTDLPLFE
                     IGGLPVAVSICEDAWQYGGPIPELGAGGARVILNINGSPYYAAKGRVRERLIGALAGA
                     ADCPIVYLNLVGGQDELIFDGDSFVCDAAGEVVARAEAFVEQVLVVDVVPGPERPVTL
                     SVTPVTAAVTPQAPLPAALPTRELSHAAEVWEAVVLGTRDYVTKNGFAGVVLGLSGGV
                     DSSLVAAIAVDAIGADKVHGVLMPSRYSSDHSVTDADRLAAALGIETRTIAIEAAHAG
                     MLTMLAESFEGREPDLTEENLQSRIRGMVLMSLSNKFGWLVLTTSNKSESAVGYATLF
                     GDTAGGFAVIKDVPKLLVYDLARYRNGVAGRELIPEAVLTKPPSAELRPDQRDDQSLP
                     PYPVLDPILELYVEDDRSIEEIVAAGHDRETVARIVGLVDRAEYKRRLTPPGPKLTTK
                     AFGRDRRLPITNRYRG"
BASE COUNT          442 a         1047 c         1015 g          404 t
ORIGIN      
        1 acgtcgtcca tggccaggtc gagccccggc tcgaccagca cgcagcggat cgccatcgtc
       61 ggagtctccc acccccgatc cgtgagaggc tcggggggtg gggacgcggc gcggggggcg
      121 ctcggtcgtg ctggtgatgg ccgtcttcgc cctggtcgcg ggctgttctg atgacaccgg
      181 cggtgaccat gcggcatcgg atgaggaccc gacggtcacc gtcgtcagcc agaacctgct
      241 gcacggcaca gcctgtgccg aggactcaga ccgctgtgac ctgcccgccc gggtccagct
      301 cttcgtcgac caactggccg acgccgggtg ccccgagctg gtcagcgtcc aggagtccaa
      361 ccagcagacg gtcgacgagt tggcgtcggc cctgggcgac ggctgcactg ccgagtacgc
      421 gatcgtctgg gacgacgacc ccacgctgga ccgtgagacc gtcctgtcca cggagccggt
      481 gctcggctcg cgccgcatcg ctctggcggg cccgctccgc agcgcctact gggtgcgggt
      541 cgcttccgac gtggggatcg tggactacat cagcagccac ctggccagca gcagcgacga
      601 ccgaccgtgc gacgaggcca cctgcccccc accctgcgag gtggccgaca cggtgaacac
      661 ctgccagggc cggcagctgg tcgagttcgc cgacgaggtg gccgatccgg aggccgtcgt
      721 cgtcatcggc ggcgacctga acgccatgcc cgacgagccc acgatcgccg ccatcaccgg
      781 ggcgggctac gtcgacaccc atctcgccgt cgggaacccg gagtgcgatc cggacacggg
      841 ccagcagtgc acgtccggac gcatcgacga cgccctgacc gacctcgagg acccggccag
      901 cctgcagtcc gagcgcatcg actacctctt cgtgggtggc ccgcgggact gcacccccgt
      961 cgagccgacc ggcctcttca acggcgagcc cgccgacggg acgctggcct tcccgtcgga
     1021 ccacaccggc gtgcagacca ccctcagttg cccgacgaac cccgagcagc tcgacgccgc
     1081 cccctcggcc accgtgcccg cggtgacgac cacgacgact gggccggcgg cgcccgtgga
     1141 cgccgagacc gaggccgcca tcacgcaggc cttcaccaac gtcttcgacg gctccgtgac
     1201 cgacgtcggc gtcaagctgg cgtccctcga gtcagccgac gagctggagc cgtacttcct
     1261 ggagagcctc gaggccaccc aggacatcgc ctcccgcatc cgcctccaga tcgacgccat
     1321 cgaggcggtc gacgccagga cggcggacgt cacctacaca ctgcttctcg acggggccgc
     1381 cgtgctcgac cacctgcctg gccaggcggt gaaggtcgac ggggagtggc tggtgtccct
     1441 gcgcacctat tgcgacgtca gcacgcaggg cgccgacacc attcccgaac cctgccagtg
     1501 aaccgtcagc cccggtagcg gttggtgatc gggagccggc ggtcccggcc gaaggccttg
     1561 gtcgtcagct tgggcccggg cggggtgagc cggcgcttgt actcggcccg atccacgagg
     1621 ccgacgatcc gcgccaccgt ctcccgatcg tggccggcgg ccacgatctc ctcgatggaa
     1681 cggtcgtcct cgacgtagag ctcgaggatc gggtccagca cggggtaggg cgggaggctc
     1741 tggtcgtcac gctggtcggg acgaagctcg gccgacgggg gcttggtcag gacggcctcg
     1801 gggatcagct cccggccggc gacgccgttg cggtagcggg ccaggtcgta gaccagcagc
     1861 ttgggcacgt ccttgatgac ggcgaagcca ccggcggtgt cgccgaagag ggtggcgtag
     1921 ccgaccgcgc tctcgctctt gttggacgtg gtcaggacca gccagccgaa cttgttgctc
     1981 agcgacatca ggaccatgcc ccggatgcgg gactgcaggt tctcctcggt gaggtccggc
     2041 tcccgaccct cgaaggactc ggccagcatc gtcaacatcc cggcgtgggc ggcctcgatg
     2101 gcgatcgtcc gggtctcgat gccgagcgcg gcggccagcc gatcggcgtc ggtcaccgag
     2161 tggtcgctcg agtaccggga gggcatgagg acgccgtgga ccttgtcggc cccgatggcg
     2221 tcgacggcga tggcggcgac cagcgatgag tcgacgccgc ctgacaggcc caacacgacc
     2281 ccggcgaaac cgttcttggt gacgtagtcc cgggtgccca gaaccacggc ttcccacacc
     2341 tccgccgcat gggagagctc ccgcgtgggc agcgcggccg gcaacggggc ctggggtgtg
     2401 accgcggcgg tcaccggggt gaccgacaac gtgaccggcc gctcgggtcc gggtacgacg
     2461 tccaccacca gcacctgctc gacgaaggct tcggcccggg ccacgacctc gccggcggcg
     2521 tcgcacacga aggagtcgcc gtcgaagatc agctcgtcct ggccgccgac caggttgagg
     2581 tagacgatcg ggcagtcggc cgccccggcc agcgccccga tcaaccgctc gcggacccga
     2641 cccttggcgg cgtagtaggg cgagccgttg atgttgagga tcacccgggc cccgcccgcc
     2701 cccagctcgg ggatcggccc gccgtactgc caggcgtcct cgcagatgga gacggcgacg
     2761 ggcaggccgc cgatctcgaa gaggggcagg tcggtgcccg gctcgaagta gcgctgctcg
     2821 tcgaacacgg cgtagttggg caggtgctgc ttgcggtaga cgccgacgac ctcgccccgc
     2881 acgcacaccg cggccgcgtt gtggagca
//
LOCUS       DASTFV010000489         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1802611, whole genome shotgun sequence.
ACCESSION   DASTFV010000489 DASTFV010000000
VERSION     DASTFV010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802611"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFP02_08925"
     CDS             <1..438
                     /locus_tag="VFP02_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543191.1"
                     /translation="KAGHPVLTELLSRIMKQEGRHIDFYASEANLRLERSRTARALTR
                     FALRRAWTPVGAGVLPDAEVAFVVQYLFDNPEGRAMVARIDRRIDRLPDQEGLGLLAN
                     ALATWRAKAAQLQVVGSTDANRSQRMADESGRTHSTRSVPHAA"
     gene            complement(336..>2079)
                     /locus_tag="VFP02_08930"
     CDS             complement(336..>2079)
                     /locus_tag="VFP02_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543192.1"
                     /translation="LMVLREGAWRNADGRLHWTLFDDVMISMSYARTLAETGELVWFA
                     GAPRTEGMTNLGWTLVMALLHRLGLSGDAVVLTVVALGLVVVWATALQARTTVGLLTG
                     ASDPMTRFLSVLAVATCFPLLWWTLRGMEVGLLVLLSLVAVNATLRLARDEGSSRRDV
                     LVLGAAVFLGIVVRSDFVVVAGGLVAWLGLRRPSRRPLRLAAGIGAVTVLTTAATVAF
                     RLVYYGDPVPNTYFLKLAGFALGARLDRGLVVTAVTVAGYLAAPLVVIALGRRRLGPV
                     GQDGARLLGLLAGLSALYGVSAGGDAWEYFLIPNRYLTPSLVLLLVLAVAVLRAGPKT
                     ATAAAGERRGLAWVVVLAACGPAVVYLTVLEAPRPDLSLVALAPGGTWLGVALPVLIV
                     GLVAGLWLGRSEVGRDRLGVVMVAVLTLAGAVAAPSLAFGGIDRAADYSVLGEQIAAI
                     TEPDARVAVVAAGATQYVSHRPAVDLLGKSDERIAKAPPATDALVLPGHNKYDFAYSI
                     GELQPDVVAQPWRELAPADLLGWGYLPYRLARGTFGEAAYAACGTDRVLWVRPDSSAI
                     RWDRLASVDPTTCS"
BASE COUNT          334 a          755 c          773 g          217 t
ORIGIN      
        1 aaggcggggc acccggtgct gaccgagctc ctgtcgcgga tcatgaagca ggaagggcgc
       61 cacatcgact tctacgcctc cgaggccaac cttcgcctcg agcggtcccg gaccgctcgg
      121 gccctgaccc gcttcgccct gcgcagggcc tggacgccgg tcggggccgg ggtcctgcct
      181 gatgccgaag tggccttcgt ggtgcagtac ctcttcgaca accccgaggg ccgcgccatg
      241 gtcgctcgca tcgaccggcg catcgaccgg ctgcccgacc aggaggggct cgggctgctc
      301 gccaacgcgc tcgcgacctg gcgggcgaag gccgctcagc tgcaggtggt gggatcgacc
      361 gacgccaacc ggtcccaacg aatggccgac gagtccgggc ggacccacag cacccggtcc
      421 gtgccacacg ccgcgtaggc ggcctcgccg aacgtcccgc gggccaggcg gtagggcagg
      481 tagccccagc cgaggaggtc ggcgggcgcc agctcccgcc agggctgggc gacgacgtca
      541 ggctggagct cgccgatcga gtaggcgaag tcgtacttgt tgtggccggg caggacaagg
      601 gcgtcggtcg ccgggggagc cttggcgatc cgttcgtcgc tcttgcccag gaggtcgacg
      661 gcggggcggt gcgacacgta ctgggtggcg ccggcggcca cgaccgcaac ccgggcgtcg
      721 ggctcggtga tcgccgcgat ctgctcgccc aggacgctgt agtcggcagc tcggtcgatt
      781 cccccgaagg cgaggctggg ggcggccacg gccccggcca acgtcaggac ggcgaccatg
      841 acgacgccga gccgatcgcg gccgacctcc gaccgcccca gccagagccc ggcgacgagg
      901 cccacgatca gcacgggcag agccacgccc agccaggtcc cgccgggggc cagcgcgacc
      961 agcgacagat ccgggcgcgg cgcctcgagc acggtcaggt agaccacggc cgggccgcag
     1021 gcggccagca ccaccaccca ggcgaggccc cgccgctcgc cggccgcggc cgtggcggtc
     1081 ttcggacccg ctcggagcac cgccacggcg aggaccagca ggaggacgag gctcggcgtg
     1141 aggtaccggt tcgggatcag gaagtactcc cacgcgtccc cacccgcgga gacgccgtac
     1201 agggcgctga gcccggccag cagccccagc aggcgggcgc cgtcctggcc gaccggcccg
     1261 agccggcgcc gacccagggc gatgacgacc agtggggcgg ccaggtagcc ggcgacggtg
     1321 accgcggtga ccacgagacc ccggtccagc cgggccccga gggcgaagcc ggcgagcttc
     1381 aggaagtacg tgttggggac cgggtcgccg tagtagacga gacggaaggc caccgtcgcc
     1441 gcggtcgtga ggacggtcac cgccccgatg ccggcggcca ggcggagggg tcgccggctc
     1501 gggcggcgca gccccagcca ggccaccagc ccgccggcga cgaccacgaa gtcggatcgg
     1561 acgacgatgc ccaggaagac cgccgccccg agcacgagga cgtcgcgccg gctgctgcct
     1621 tcgtcgcggg cgaggcgcag cgtggcgttg acggcgacca gggacaacag gacgaggagc
     1681 ccgacctcca tgccccgcag ggtccaccac agcagcggga agcaggtggc cacggccaga
     1741 acggacagga agcgggtcat cgggtcgctc gcccccgtga gcaggccgac ggtcgtgcgg
     1801 gcctgcagag ccgtggccca gacgaccacg aggccgaggg ccaccaccgt gaggaccacg
     1861 gcgtcccccg acagccccag ccggtgcagg agggccatga cgagggtcca acccaggttt
     1921 gtcatccctt cggtccgggg cgctccggcg aaccagacca gctcgccggt ttcggcgagc
     1981 gtgcgggcgt aggacatcga gatcatgacg tcgtcgaaca gcgtccagtg caggcggccg
     2041 tcggcattgc gccacgcccc ttcccgcaag accatcagg
//
LOCUS       DASTFV010000490         3306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1807149, whole genome shotgun sequence.
ACCESSION   DASTFV010000490 DASTFV010000000
VERSION     DASTFV010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807149"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /gene="prcB"
                     /locus_tag="VFP02_08935"
     CDS             <1..507
                     /gene="prcB"
                     /locus_tag="VFP02_08935"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HET9543193.1"
                     /translation="MVKLFQLQLEHYEKVEGEQLSLEGKANQLSMMVRSNLPAAMQGL
                     AVVPLFAGYDLGRGIGRLFEFDVTGGRYEENDHASTGSGSLHAGTVVKLGWKPDLTRE
                     EAVDLALKALFQAADEDSATGGPDLVRGIYPTMATITAEGFTALERDEIAERFRALLD
                     SMDGGLAS"
     gene            504..1262
                     /gene="prcA"
                     /locus_tag="VFP02_08940"
     CDS             504..1262
                     /gene="prcA"
                     /locus_tag="VFP02_08940"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HET9543194.1"
                     /translation="MSMPFYVAPEQRMKDLADYARKGIARGRSLVAVGYADGIAIVAE
                     NQSNTLRKISEIYDHIAFAGVGKYNEFDQLRIAGVRHADLKGYSYSREDVNARGLANQ
                     YAQILGQIFTHDLKPMEVEILVAEIGPDPRHDQMFHILYDGTVRDENQCTALGGEADP
                     ILDRLRETWSEGEDLDVALTRAIEALTGPDRRLSADDLEVALLDRTTGQRRAFRRLEP
                     EELRSRLGDLVAATEGNGGGVDLEPPPPDDEAQA"
     gene            complement(1253..1507)
                     /locus_tag="VFP02_08945"
     CDS             complement(1253..1507)
                     /locus_tag="VFP02_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543195.1"
                     /translation="MADLHMVHLASVDTPFEARVLVARLGAEGIVWELRGASDVYPVG
                     QTHVWVDEASVGTARELLLTDAVEAVFDDDSDGGDGLGQA"
     gene            1571..2929
                     /gene="pafA"
                     /locus_tag="VFP02_08950"
     CDS             1571..2929
                     /gene="pafA"
                     /locus_tag="VFP02_08950"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HET9543196.1"
                     /translation="MDRRIFGLENEYGVTCTLRGQRRLSPDEVARYLFRRVVSWGRSS
                     NVFLANGARLYLDVGSHPEYATPECDSIYDLVVHDKAGERILEQLLASAEQRLREEGI
                     RGVIYLFKNNTDSAGNSYGCHENYLTSRRDDFSHYVDVLIPFLVSRQIYAGAGKVLQT
                     ARGAMFCISQRAEHIWEGVSSATTRSRPIINTRDEPHADAERYRRLHVIVGDSNMSEY
                     ATFLKVGAASIILRMLEEPSVVIRDMTLENPIRAIREISHDLTCTRRVRLANGREASA
                     LEIQSEYLNRAMRFADTRGLSPLEQQALDMWEHTLTGLEKDPFSLDRECDWVIKHNLI
                     EAYRERHDLPLSHARVALLDLQYHDVNRNRGLFYRMQQKGMVDRVATDQDINHAVEHP
                     PQTTRARLRGEFIKKAKEKKRDYTVDWVHLKLNDQAQRTVLCKDPFKARDERVEKLIA
                     SL"
     gene            2940..>3306
                     /locus_tag="VFP02_08955"
     CDS             2940..>3306
                     /locus_tag="VFP02_08955"
                     /inference="COORDINATES: protein motif:HMM:NF024384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HET9543197.1"
                     /translation="MPSFRTGAVTELLSERAGLQRIRVDLGEAEPARAYVLIQLTGPV
                     AVGDRVVCNTTAVELGLGTGGWHVVHWNLARDDWSEPGAGHIMKLRYTSLQVDTGAGE
                     EDHPEVPPRLDGTPVVVCTV"
BASE COUNT          577 a         1242 c         1043 g          444 t
ORIGIN      
        1 atggtcaagc tcttccagct ccagctcgag cactacgaga aggtcgaggg cgagcagctc
       61 tccctggagg gcaaggccaa ccagctctcg atgatggtgc gcagcaacct gcccgccgcc
      121 atgcagggcc tggccgtcgt gccgctgttc gccggctacg acctgggccg cggcatcggc
      181 cgcctcttcg agttcgacgt caccggcggg cgttacgagg agaacgacca cgccagcacc
      241 gggtcgggct cgctccatgc ggggacggtc gtgaagttgg gctggaaacc cgacctcacc
      301 cgcgaagaag ccgtcgacct ggccctcaag gccctgttcc aggccgccga cgaggactcg
      361 gcgaccggcg gccccgatct cgtccggggc atctacccga ccatggccac catcaccgcc
      421 gagggcttca ccgccttgga gcgggacgag atcgccgaac ggttccgggc cctgctcgac
      481 tcgatggacg gagggctggc ctcatgagca tgcccttcta cgtcgccccc gagcagcgca
      541 tgaaggacct ggccgactac gcccgcaagg gcatcgcccg gggtcggagc ctggtcgccg
      601 tgggctacgc cgacggcatc gccatcgtgg ccgagaacca atccaacacc ctgcgcaaga
      661 tcagcgagat ctacgaccac atcgccttcg ccggcgtcgg caagtacaac gagttcgacc
      721 agctccgcat cgccggcgtc cgccacgccg acctcaaggg ctacagctac agccgcgagg
      781 acgtgaacgc ccggggcctg gccaatcaat acgcccagat cctcggccag atcttcaccc
      841 acgacctgaa gcccatggag gtggagatcc tcgtggccga gatcggcccg gacccgagac
      901 acgaccagat gttccacatc ctctacgacg gcaccgtccg ggacgagaac cagtgcaccg
      961 ccctaggcgg ggaggccgac cccatcctcg accggctacg cgagacgtgg agcgaggggg
     1021 aggacctcga cgtcgccctc acccgggcca tcgaagccct caccggaccc gaccggcgcc
     1081 tgtcggccga cgacctcgaa gtggccctgc tcgaccggac caccggccag cgacgagcct
     1141 tccgccgcct ggagcccgag gagctgcgtt cgcggctggg cgacctggtc gccgccacgg
     1201 agggcaacgg cggtggcgtc gacctcgaac caccgccgcc cgacgacgag gctcaggcct
     1261 gacccaggcc gtcgccgcca tcgctgtcgt cgtcgaagac ggcctcgacg gcatcggtca
     1321 acagcagttc ccgggcggtg cccacactcg cttcgtccac ccagacgtgg gtctggccca
     1381 ccggatagac gtcggaagct ccccgtagct cccacacgat gccctccgcc ccgagccggg
     1441 ccacgagcac tctcgcctcg aacggcgtgt cgacgctggc caggtggacc atgtggaggt
     1501 cggccacccc gcaaccgtac cggcgcagca tggtccaccc cggagccgca ctaccggtag
     1561 cgtgccgcgc atggaccggc ggatcttcgg gctcgagaac gagtacgggg tcacctgcac
     1621 gctgcgcggc cagcgtcggc tgagtcccga tgaggtcgcc cgctacctct tccgccgggt
     1681 cgtgtcctgg ggtcgcagct cgaacgtctt cctggccaac ggcgcccgcc tctacctcga
     1741 cgtgggcagc caccccgagt acgccacccc cgagtgcgac tccatctacg acctcgtcgt
     1801 gcacgacaag gcgggtgagc gcatcctcga acagctcctg gcctcggccg agcagcgcct
     1861 ccgggaagag ggcatccggg gcgtcatcta cctgttcaag aacaacaccg actccgccgg
     1921 caactcctat ggctgccacg agaactacct gaccagccgc cgggacgact tcagccacta
     1981 cgtcgacgtc ctcatcccgt tcctggtcag ccgccagatc tacgccgggg cgggcaaggt
     2041 cctgcagacc gcccggggtg ccatgttctg catcagccag cgggccgagc acatctggga
     2101 gggcgtctcc agcgccacca cccgatcccg tcccatcatc aacacccggg acgagcccca
     2161 cgccgacgcc gagcgctacc ggcgcctcca cgtcatcgtg ggcgactcca acatgagcga
     2221 gtacgccacc ttcctcaagg tgggggcggc cagcatcatc ctgcgcatgc tcgaggagcc
     2281 atcggtcgtc atccgggaca tgaccctgga gaaccccatc cgggccatcc gcgagatcag
     2341 ccacgacctg acctgcaccc ggcgggtccg cctggccaac ggccgggagg cctcggcgct
     2401 ggagatccag tccgagtacc tgaaccgggc catgcgcttc gccgacaccc gggggctgtc
     2461 gccgctggag cagcaggccc tggacatgtg ggagcacacc ctcaccggcc tcgagaagga
     2521 ccccttctcg ctcgaccggg agtgcgactg ggtcatcaag cacaacctca tcgaggccta
     2581 ccgggagcgc cacgacctgc ccctgtccca cgctcgggtc gccctgctcg acctgcagta
     2641 ccacgacgtc aaccgcaacc ggggcctctt ctaccggatg cagcagaagg gcatggtcga
     2701 ccgggtcgcc accgaccagg acatcaacca cgccgtcgag cacccgccgc agaccacgcg
     2761 ggcccgcctc cggggtgagt tcatcaaaaa ggccaaggag aagaaacggg actacacggt
     2821 ggactgggtc cacctgaagc tgaacgacca ggcccagcgc accgtcctgt gcaaggaccc
     2881 cttcaaggcc cgtgacgagc gggtcgagaa gctgatcgcg tccttgtagc cccgccgccg
     2941 tgccctcgtt ccgcaccggc gcggtcaccg aactgctcag cgaacgcgcc gggctccagc
     3001 gcatccgggt ggacctcggc gaggccgagc cggcgcgggc ctacgtcctg atccagctca
     3061 ccggcccggt cgcggtgggg gaccgggtcg tctgcaacac cacggccgtc gagctcgggc
     3121 tgggcactgg cggctggcat gtcgttcact ggaacctggc ccgcgatgac tggagcgagc
     3181 ccggcgccgg ccacatcatg aagctgcgct acaccagcct ccaggtcgac acgggcgcgg
     3241 gggaggagga ccaccccgag gtcccgcccc gcctcgacgg cacgcccgtg gtggtctgca
     3301 ccgtgc
//
LOCUS       DASTFV010000491         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1807753, whole genome shotgun sequence.
ACCESSION   DASTFV010000491 DASTFV010000000
VERSION     DASTFV010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807753"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..2145
                     /locus_tag="VFP02_08960"
     CDS             106..2145
                     /locus_tag="VFP02_08960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HET9543198.1"
                     /translation="MAVVDTPTPHRKAEPVVIDLRTRRRVGVRLPRLPVRPGPAPTSP
                     PPAGALTYQPAIDGLRGLAVAGVLLFHGGFSWAKGGYLGVSTFFTLSGYLITTLLLAE
                     FGRTRRVSLPGFWSRRFRRLMPASLLTLAGIVVLFGPFVATADQLAELPGDVIAALAY
                     VANWRFIVADQSYADLFTAPSPVQHFWSLAIEEQYYLFFPLLMAGLLAFGRGSRRLVF
                     MVLGALTLGSTLLMMSLYTPGTDPARVYYGTDTRAAELLIGALLAVLLAARPHLLARI
                     PRRALTAAGTAVLVVTAYWWMTVEQISPWLYQGGFTFYALTTAVLLAVLLVPGPVQRA
                     LATDPLRQLGRISYGVYLLHWPIFLWLTPERTGLSIVPLFVLRLAVTIPAAVLSFRFV
                     EQPVRTRQILVGPRYRLVAPAAVLVIVFGVVALDTTTDEQQDDSLGAILDASPPPQDP
                     EELLAATPDAAGTSTVPTVDRVLLVGDSVMGQAYEVFRGVFDDQGIATGYAGGPSTGP
                     LQPQGDWARQIDDWVTRFDPDVVVMEACCDYTRTTDQVYVDPEGNEVLPNTEEVYPNW
                     EREVRDLIRRARAGGAQVVWVLSPPVQTNGFYGPLEEHVRRLNQMYRNLPVRTLDWGR
                     VITPGGTYTESVVGPDGETEVVRLADGVHMTEFGNELLADLTLEQVELVGEHPVF"
BASE COUNT          245 a          834 c          707 g          362 t
ORIGIN      
        1 cgccgccccc tggtgttctc gagtcgggcc cgggtcctgg ccctccggga ccacgtccgt
       61 gggcgctatg ggccgccgcc gcctggcttg taggttggcg ctcccgtggc cgtggtcgac
      121 acccccaccc ctcaccggaa agccgagccg gtcgtcatcg acctccggac ccgacgacgg
      181 gtcggggttc gcctgccgcg cctccccgtg cgcccgggcc ccgcgccgac gagcccacct
      241 cctgcgggcg cgctcaccta ccagccggcc atcgacgggc tgcggggcct ggccgtggcc
      301 ggcgtgctcc tcttccatgg cgggttctcg tgggccaagg ggggctacct cggggtctcc
      361 accttcttca ccctctcggg ttacctgatc acgaccctgt tgctggccga gttcggccgt
      421 acccgccgcg tctcgctgcc ggggttctgg agccggcggt tccggcgcct gatgcccgcc
      481 tcgctgctga ccctggccgg catcgtcgtc ctcttcgggc ccttcgtggc cacggcggac
      541 cagttggccg agctgcccgg cgacgtgatc gcggcgctgg cctacgtggc caactggcgc
      601 ttcatcgtgg ccgaccagtc ctacgccgac ctgttcacgg ccccctcccc ggtccagcac
      661 ttctggtcgc tggccatcga agagcagtac tacctgttct tcccgctcct catggccgga
      721 ctgctggcct tcggccgggg ctcgcgccgc ctcgtgttca tggtcctcgg ggccctgacc
      781 ctgggctcga ccctgttgat gatgagcctc tacacgccgg gcaccgatcc cgcccgggtg
      841 tactacggca ccgacacccg ggccgccgag ctgctcatcg gggccctgct cgccgtgctc
      901 ctggccgccc gcccgcacct gctggcccgc atcccgcggc gggcgctgac cgcggcgggg
      961 accgccgtgc tggtggtcac cgcgtactgg tggatgacgg tcgagcagat ctccccgtgg
     1021 ctctaccagg gcggcttcac gttctacgcc ctgaccaccg ccgtgctcct cgccgtcctg
     1081 ctggtgccgg gtccggtcca gcgggcgctc gccaccgacc cgctccgcca gctcggccgc
     1141 atctcctacg gcgtctacct cctgcactgg cccatcttcc tctggctcac cccggagcgg
     1201 accggactgt ccatcgtccc cctgttcgtc ctgcggctgg ccgtgaccat ccctgcggcc
     1261 gtcctctcgt tccgcttcgt cgagcagccg gtgcggacgc ggcagatcct ggtcgggccc
     1321 cggtaccgcc tggtggcacc cgccgccgtc ctcgtcatcg tcttcggcgt cgtcgccctc
     1381 gacaccacca ccgacgagca gcaggacgac agcctcggtg cgatcctgga cgcctccccg
     1441 ccgccccagg accccgagga gctgctcgcg gcgacgcctg acgccgccgg caccagcacc
     1501 gtgcccaccg tcgaccgggt cctgctcgtc ggcgactccg tgatggggca ggcgtacgag
     1561 gtgttccggg gtgtgttcga cgaccagggg atcgccaccg gctacgccgg tgggccgagc
     1621 accgggccgc tccagccgca gggcgactgg gcccgccaga tcgacgactg ggtgacccgt
     1681 ttcgaccccg acgtcgtggt catggaggcc tgctgtgact acacccggac caccgatcag
     1741 gtctacgtcg acccggaggg caacgaggtc ctgcccaaca ccgaggaggt gtatccgaac
     1801 tgggagcggg aggtgcggga cctgatccgc cgcgcgaggg cgggcggggc ccaggtcgtc
     1861 tgggtcctgt cgcctccggt gcagaccaac ggtttctacg gcccgttgga ggagcacgtg
     1921 cgccgcctca accagatgta ccggaacctg ccggtgcgga ccctggactg gggtcgggtc
     1981 atcacccccg ggggcaccta taccgagtcg gtcgtcggtc ccgacggcga gaccgaggtg
     2041 gttcgcctgg ccgacggcgt ccacatgacc gagttcggca acgagctcct ggccgatctc
     2101 accctcgagc aggtcgagct ggtgggcgag caccccgtct tctgaccc
//
LOCUS       DASTFV010000492         2801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1808257, whole genome shotgun sequence.
ACCESSION   DASTFV010000492 DASTFV010000000
VERSION     DASTFV010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808257"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /gene="uvrB"
                     /locus_tag="VFP02_08965"
     CDS             complement(<1..1583)
                     /gene="uvrB"
                     /locus_tag="VFP02_08965"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HET9543199.1"
                     /translation="MAREDRFEMVADFAPAGDQPQAIAGLSEGLAAGEKFQTLLGITG
                     SGKSATIGWTIEQFQRPTLVMAPNKSLAAQLANEFRAFFPKNKVEYFVSYYDYYQPEA
                     YLPSSDTYIEKDSSVNDEIERLRHATTASLLTRRDVIVVASVSAIYGLGSPEEYRNQL
                     LYVRVGEEHDMRSILRKLVDIRYERNDLNLTRGRFRVRGDTLEVHPAYEEHAVRIEMF
                     GDEIERIVLVDPLTGEQLDVVDELMILPASHYVTGDERMRLAIQRIEVELQERLKEFE
                     EQGKLLEAQRLRMRTSYDLEMMQEVGFCNGIENYSGPIDGRARGEAPNTLLDFFPDDF
                     LMVLDESHVMVPQLHGQYEGDRTRKETLVEHGFRLPSAADNRPLTFDEFMSKVNQVVF
                     LSATPSPWEVERSSRVVEQIVRPTGLIDPEVIVKPTKGQIDDLMEQIKIRTTAGDRVL
                     VTTLTKKMAEDLTDYLLELGVRVRYLHSEVDTIERIELLRDLRLGEFDVLVGINLLRE
                     GLDLPEVSLVAILDADKEG"
     gene            1666..>2801
                     /locus_tag="VFP02_08970"
     CDS             1666..>2801
                     /locus_tag="VFP02_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543200.1"
                     /translation="MAVITRTDPETTAAPSSSVSSGRGLVVLFTVASFVGSTLLFLVQ
                     PLVAKLLTPLLGGTPDVWNTAMVFFQATLLLGYGIAHLGARWLRGRQIPVHVVLVAIP
                     LLALPLAVPEGWRLPIGTAPALWTLLVLAITVGLPFLALSTASPTFQRWFSLSGHRHA
                     ADPYFLYAAGNVGSLLALLAYPLLLEPHLSTSAQSRLWSMGYLAFVLLTGACAWVIQR
                     RSAATAVATIIDAGPTSPPLGSRLRLRLIGFAFVPSLLLLGVTRHLSTDIASIPLLWV
                     VPLALYLLSFVLAFGRPPARLIDLSGRTVALLAVPLAAAFAASFDSVWLVLIPHLVFF
                     LAAATLAHARLAELRPPADRLTEFYLWISLGGVLGGIAGALIAPV"
BASE COUNT          350 a         1016 c          920 g          515 t
ORIGIN      
        1 aagccctcct tgtcggcgtc gaggatggcc accagcgaca cctcgggcag gtcgaggccc
       61 tcccgcagga ggttgatgcc tacaaggacg tcgaactcgc ccagacgcag gtcccggagc
      121 agctcgatgc gctcgatggt gtcgacttcg gagtgcagat agcggacccg gacgccgagc
      181 tcgagcaggt agtcggtcag gtcctcggcc atcttcttgg tcagggtcgt gaccaggacc
      241 cggtccccgg ccgtggtccg gatcttgatc tgctccatca ggtcgtcgat ctggcccttg
      301 gtgggcttca cgatgacctc ggggtcgatg aggcccgtgg ggcggacgat ctgctccacc
      361 acccggctgg agcgctccac ctcccagggg ctgggggtgg ccgacaggaa cacgacctgg
      421 ttcaccttgc tcatgaactc gtcgaaggtg aggggccggt tgtcggccgc cgacggcagg
      481 cggaagccgt gctcgacgag ggtctccttg cgggtgcggt ccccttcgta ctggccgtgg
      541 agctggggga ccatgacgtg ggactcgtcc agcaccatca ggaagtcgtc ggggaagaag
      601 tcgagcaggg tgttgggggc ctcgccccgg gcccgaccgt cgatgggacc ggagtagttc
      661 tcgatcccgt tgcagaagcc gacctcctgc atcatctcca ggtcgtagct ggtgcgcatg
      721 cgcaggcgct gggcctcgag cagcttgcct tgctcctcga actccttcag ccgctcctgc
      781 agctcgacct cgatgcgctg gatggccagg cgcatccgct cgtcgccggt gacgtagtgg
      841 ctcgccggca ggatcatcag ctcgtcgacg acatcgagct gctcgccggt gagcgggtcg
      901 accagcacga tgcgttcgat ctcgtcgccg aacatctcga tgcggacggc gtgctcctcg
      961 taggcggggt gcacctcgag ggtgtcgccc ctcacccgga agcggccccg ggtcaagttc
     1021 aggtcgttgc gctcgtagcg gatgtcgacc agcttccgca ggatcgagcg catgtcgtgc
     1081 tcttcgccca ctcggacgta gaggagctgg ttccggtact cctcgggcga gcccagcccg
     1141 tagatggcgg acaccgaggc gaccacgatg acgtcccgcc gcgtcagcag ggaggccgtc
     1201 gtggcgtggc ggaggcgctc gatctcgtcg ttgaccgacg agtccttctc gatgtaggtg
     1261 tcgctcgacg gcaggtacgc ctcgggctgg tagtagtcgt agtaggagac gaagtactcg
     1321 accttgttct tcgggaagaa ggcccggaac tcgttggcca gctgggcggc gagcgacttg
     1381 ttcggggcca tgaccagcgt cgggcgttgg aactgctcga tggtccaccc gatggtcgcg
     1441 ctcttgccgc tgccggtgat gcccagcagg gtctggaact tctcccccgc ggccaatccc
     1501 tcgctcagcc cggcaatggc ctggggctgg tcgccggctg gggcgaagtc ggccaccatc
     1561 tcgaagcgat cttcacgggc cacccgggca gggtaccgac gggctgtgac agcaccgggc
     1621 cttcggccgg ctcagggtgg gtcggtaccc gccgacggga gcgccgtggc cgtgatcacc
     1681 cgcacagacc ccgaaacgac ggccgcgccg tcgtcgtcgg tgtcctcggg ccgcggcctg
     1741 gtcgtgctgt tcaccgtggc cagcttcgtc ggctccaccc tgctgttcct cgtccagccc
     1801 ctcgtggcca agctgctgac cccactgctg ggcggcaccc cggacgtgtg gaacacggcc
     1861 atggtcttct tccaggccac cctcctcctc ggctacggca tcgcccacct gggggcccgg
     1921 tggctgcgcg gccgtcagat cccggtccac gtcgtcctcg tcgccatccc gctgctggcc
     1981 ctgcccctcg ccgtccccga gggttggcgc ctgccgatcg gcaccgcgcc ggccctgtgg
     2041 accctgctag tcctggccat caccgtgggc ctgcccttcc tggccctctc caccgccagc
     2101 cccacgttcc aacgttggtt ctcgctgagc gggcaccgtc acgcagcgga cccgtacttc
     2161 ctctacgccg cgggcaacgt gggcagcctc ctcgccctgc tggcctaccc gctgctcctc
     2221 gagccccacc tgtcgacgtc ggcccagagc cgcctgtgga gcatgggcta cctcgctttc
     2281 gtgctgctca ccggggcctg cgcctgggtg atccagcgcc ggtccgcggc caccgccgtg
     2341 gccacgatca tcgacgccgg accgacctcg ccgcccctgg gctcccgcct acggctccgg
     2401 ctgatcgggt tcgccttcgt ccccagcctg ctcctgctcg gggtcacccg gcacctgagc
     2461 accgacatcg cctccatccc gttgctctgg gtcgtgcccc tggcgctcta cctgctcagc
     2521 ttcgttctcg ccttcggtcg cccgccggct cgcctgatcg acctctcggg tcggaccgtg
     2581 gccctgctgg cggttcccct ggcggcggcc ttcgccgcct cgttcgactc ggtgtggctg
     2641 gtgctgatcc cccacctcgt cttcttcctc gccgccgcca ccctggccca cgcccgcttg
     2701 gccgagctcc ggccgccggc tgaccgcctg accgagttct acctgtggat ctccctcggc
     2761 ggggtcctcg gcggcatcgc cggagccctg atcgcgccgg t
//
LOCUS       DASTFV010000493         3607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1809443, whole genome shotgun sequence.
ACCESSION   DASTFV010000493 DASTFV010000000
VERSION     DASTFV010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809443"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VFP02_08975"
     CDS             complement(<1..730)
                     /locus_tag="VFP02_08975"
                     /inference="COORDINATES: protein motif:HMM:NF014764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543201.1"
                     /translation="MPDDRRVDGLTGARVRVVAARQDRPNQPLTDQCPFCVGGLEAPA
                     PYAVKAFPNRWPPMPDQRAEIVLYTPDHDATFAALGQPGARRVIDLWAERTATLGARD
                     DVVYVLVFENRGPEVGATIPHPHGQIYAYDEIPPVPARELAADGCSLCAAPLADLLVS
                     RAGGWRVSVPAASEWPYGLLVAPDAHLPDLPSLTDDDRGGLADALVDAFGRLDRLFSA
                     PMPYMAWFHQRPTDGGTWPNAHVHL"
     gene            complement(730..1632)
                     /locus_tag="VFP02_08980"
     CDS             complement(730..1632)
                     /locus_tag="VFP02_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET9543202.1"
                     /translation="MSPLWGVHTGLQNTTVTELQDLWRRIEDHGFDWISIWDHFYAAD
                     LAGTHCLEAVAAHTALAMSTERVRVGALVYCAGYRHPAVLAKAIATIDHFSGGRADIG
                     LGAGWASGEYHAYGIPYPGNGARLDILEEAVQCIRGLLRQDRTDFAGDLFTLTDAACD
                     PKPLQAELPIWIGGGGEKRTLAIAARYADGWNIPLVAPEVFARKKGVLAEHCDRVGRD
                     PGEIRCAVNVGAAVDDAALRAQFGGMTDYARPGCLVGSPDEMVDQVGAFVDAGADQIN
                     LALRAPWDLAVLDHLATAKDQFAP"
     gene            1840..2919
                     /locus_tag="VFP02_08985"
     CDS             1840..2919
                     /locus_tag="VFP02_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543203.1"
                     /translation="MLRGDDDWARRGSPALAWTGERLFVYGGNPVPSERESARTVEPL
                     NDAALIDPANGDVDQLPDPPFDRPLRLTPAAVAVDDEVVVVGQVCRETDHDDRACDAG
                     AYRGAVYSVTDDDWREIDLPNRLKQISNGQSSAVGTTSDGRAVLALGARGGFGAIANR
                     EIWTYSPADDEWEQLPPPGSLIEGVCMAGDAVVVGSGLLPQTAAGDTGAPVTPTEPAA
                     VGPSLAVLALDGDARVWIPTGPAGVLPTADQASITCGTDLVLVDGGAEALRVFDLGVD
                     GGWRTPAPRPGDDVHASRLWTGEEFLFLDANAPTLGYDPVADTWRGIENSAPTGLRSV
                     WTGDSVVGWPGRTDTPVEFDVDGDP"
     gene            complement(2949..3380)
                     /locus_tag="VFP02_08990"
     CDS             complement(2949..3380)
                     /locus_tag="VFP02_08990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9543204.1"
                     /translation="MILRTARFADLDATTLYAVLKLRTDVFVVEQDCPYPELDGRDAE
                     PEAEHRWFEDEAGSPVAYLRTLVESDGATRIGRVVTRPDARGTGLARQLVEGVLADHL
                     GPVVADAQAHLVGWYEALGFVITGPEYLEDDIAHVPMRREP"
     gene            3412..>3607
                     /locus_tag="VFP02_08995"
     CDS             3412..>3607
                     /locus_tag="VFP02_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET9543205.1"
                     /translation="MGPLSGVKVIELAGIGPGPFTAMVLGDMGADVIRIDRSVASLGG
                     DPADPPKDILTRGRRSIGLDL"
BASE COUNT          485 a         1255 c         1366 g          501 t
ORIGIN      
        1 ggaggtggac gtgggcgttg ggccaggtcc caccgtcggt cgggcgctgg tggaaccagg
       61 ccatgtaggg catcggagcg gagaaaaggc ggtccaagcg gccgaaggcg tcgaccaagg
      121 cgtcggccag gccgccccgg tcgtcgtcgg tcagcgacgg cagatccggg aggtgagcat
      181 cgggggcgac aagcagcccg tagggccact cggacgccgc ggggacactc acccgccaac
      241 cgcccgctcg gctgaccagc aggtcggcaa ggggcgccgc gcagagcgag cagccgtccg
      301 ctgccagctc ccgggccggg accgggggga tctcgtcgta ggcgtagatc tggccgtggg
      361 ggtgggggat ggtggcgccc acctcgggtc cccggttctc gaatacgagc acgtagacca
      421 cgtcgtcgcg ggccccgagg gtggcggtcc gctcggccca caggtcgatt acccgccggg
      481 ctcccggctg gccgagcgcg gcaaaggtgg cgtcgtggtc cggcgtgtag aggacgatct
      541 cggctcgctg gtcgggcatc ggcggccagc ggttggggaa cgccttcacc gcgtagggcg
      601 ccggggcctc gagcccgccg acacagaacg ggcactgatc ggtaaggggt tggttgggac
      661 ggtcttgccg ggccgccacc acccgcaccc gggccccggt caacccatcg acccgccgat
      721 cgtcgggcat cagggggcga actggtcctt ggcggtggcc aggtggtcga ggacggcgag
      781 gtcccacggc gcccggagcg ccaggttgat ctggtcggcg ccggcgtcca cgaacgcccc
      841 gacctggtcg accatctcgt cgggcgagcc caccaggcaa cccggccggg cgtagtccgt
      901 catgcccccg aactgcgccc ggagggcggc atcgtcgacc gcggcaccga cgttgaccgc
      961 acaccggatc tcgcccggat cccgtccgac ccggtcgcag tgctcggcca ggacgccctt
     1021 cttgcgggcg aagacctcgg gggccacgag ggggatgttc cagccgtcgg cgtagcgggc
     1081 ggcgatggcg agtgtccgct tctcgccccc gccgccgatc cagatgggga gctcggcctg
     1141 cagcggcttg gggtcacagg ccgcgtcggt cagggtgaac aggtcgccgg cgaagtccgt
     1201 ccggtcctga cggagcagac ctcggatgca ctgcaccgcc tcctccagga tgtccaggcg
     1261 ggccccgttg ccggggtagg ggatgccgta ggcgtggtac tcgccgctgg cccacccggc
     1321 cccgaggccg atgtcggccc ggccgcctga gaagtggtcg atggtggcga tcgccttcgc
     1381 cagcacggcc gggtgccggt aaccggcgca atagaccaac gcgccgaccc ggacccgctc
     1441 ggtgctcatg gccagggcgg tgtgggcggc cacggcctcc aggcagtggg tgccggcgag
     1501 gtcggcggcg tagaagtggt cccatatcga gatccagtcg aagccgtggt cctcgatccg
     1561 gcgccagagg tcctggagct cggtcaccgt cgtgttctgc aggccggtgt ggactcccca
     1621 gagcgggctc atgggtcgga acgtagtggg gacgccggac gaacgggggc tccttgaagg
     1681 cagcgacggg gaccttgctg cctaacctca gcgtccggcg gcacggcctg gaggtgggca
     1741 ctgattttcc gacagcggtc atggggcagc gacggcacct ggtacgggtg gctgatcgtc
     1801 ctcggtgtcg cggcggcgat cctctgggtc tccatcggtg tgctccgcgg tgacgacgac
     1861 tgggctcggc ggggctcgcc cgcgctggcc tggaccggcg agcgcctgtt cgtctacggc
     1921 ggcaaccccg tgccctccga gcgcgagagc gcccggaccg tcgagccgct gaacgacgct
     1981 gccctcatcg accccgcgaa cggggatgtc gaccagctgc ccgacccccc gttcgaccgg
     2041 ccgctgcggt tgaccccggc cgcggtcgcc gtcgacgacg aggtggtggt cgtcggccag
     2101 gtctgccggg agaccgacca cgacgaccgg gcctgcgacg ccggcgcgta caggggggcg
     2161 gtctactcgg tgaccgatga cgactggcgg gagatcgacc tccccaaccg cctcaagcag
     2221 atcagcaacg gccagagcag tgccgtcggg accacctccg acggccgggc cgtcctcgcg
     2281 ctcggggccc gcggcggctt cggcgccatc gccaaccggg agatctggac ctattcgccg
     2341 gccgacgacg agtgggagca gctgccgccg cccggctcgc tcatcgaggg cgtctgcatg
     2401 gccggcgatg ccgtcgtcgt gggctcaggc ctgcttcccc agaccgccgc gggagacacc
     2461 ggagcaccgg tgaccccgac cgagccggcc gcggtcggac cgtccctggc tgtcctggcc
     2521 ctcgacgggg acgcccgggt gtggatcccc accggcccag ccggggtgtt gccgaccgcc
     2581 gaccaggcca gcatcacctg tggcaccgac ctggtcctcg tggacggtgg cgcggaggcc
     2641 ctgcgggtct tcgacttggg cgtcgacggc ggctggcgga cgccggcgcc caggcccggt
     2701 gacgacgtgc acgcctcgcg gctctggacg ggcgaggagt tcctcttcct cgacgccaac
     2761 gccccgacgc tgggctacga cccggtggcc gacacctggc ggggcatcga gaactccgcc
     2821 cccaccggtc tccggtcggt gtggaccggt gattccgtcg tcggctggcc cggccgcacc
     2881 gacacccccg tcgagttcga cgtcgacggg gatccctagt cgcgcctctc ggcccccact
     2941 ggcgcccatc agggctcgcg gcgcatgggg acgtgggcga tgtcgtcctc gaggtactcg
     3001 gggccggtga tcacgaagcc gagggcctcg taccagccga cgaggtgggc ttgggcgtcg
     3061 gcgaccacgg ggccgaggtg gtcggcgagg acgccctcga cgagctgtcg ggccaggccg
     3121 gtgccgcggg cgtcgggacg ggtcacgacc cggccgatgc gggtggcgcc gtcggactcg
     3181 accagcgtgc gcaggtaggc caccggcgat ccggcctcgt cctcgaacca gcggtgctcg
     3241 gcctcgggct cggcgtcccg cccgtcgagc tcggggtagg ggcagtcctg ctccaccacg
     3301 aacacatccg tccgcagctt gagcacggcg tagagggtgg tggcgtcgag gtcggcgaac
     3361 cgggcggtgc ggaggatcat cgggtcgatt ctcgcccgta cgatgccggg catgggtcca
     3421 ctctcggggg tcaaggtcat cgagctggcc ggcatcgggc ccggcccgtt cacggcgatg
     3481 gtgctggggg acatgggcgc cgacgtcatt cgcatcgacc ggtcggtggc gtccctgggc
     3541 ggcgaccctg ccgatccgcc caaggacatc ctgacccggg gacggcggtc gatcggcctc
     3601 gacctga
//
LOCUS       DASTFV010000494         2783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1813165, whole genome shotgun sequence.
ACCESSION   DASTFV010000494 DASTFV010000000
VERSION     DASTFV010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813165"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..1039
                     /locus_tag="VFP02_09000"
     CDS             218..1039
                     /locus_tag="VFP02_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543206.1"
                     /translation="MRTEVERLTLDLVDPSRPTPAGAGLPHADDRRLVTDVYVPAGRG
                     PFPFIAFSHGLNGHPRKFTQLLGQWAEAGYAVAAPAFPLSNDEVPGVSTFADLPSQPG
                     DVSFVIDQLLAMSEDESDALYGVVDPDRIGVAGLSLGGATTYGVAFNDCCLDERPVAA
                     MVLDGARLAVGGEFHMDSGLPLMIIHADEDFALPYAEAADAYVDAIAPKYLVTLHEFA
                     HASPYENDPDPADELVTASTIAFWDRYLKDDESADQRIVDAITPSSLATLESETG"
     gene            complement(1097..1342)
                     /gene="rsrA"
                     /locus_tag="VFP02_09005"
     CDS             complement(1097..1342)
                     /gene="rsrA"
                     /locus_tag="VFP02_09005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HET9543207.1"
                     /translation="MSDKCDETLAELYTYLDGELTDEAREKIRFHLDDCPPCGDIAGF
                     ESELRKVIASKCIDRVPDDLRARIVAACSEDPAPTTP"
     gene            complement(1347..1937)
                     /locus_tag="VFP02_09010"
     CDS             complement(1347..1937)
                     /locus_tag="VFP02_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9543208.1"
                     /translation="MADQATFSEQAMPLMDGLYGAALRMTRNPADAEDLVQETYLKAY
                     RAFHTFQQGTNLKAWLYRILTNTYINTYRAKKRRPDETDLDEVEDLYLYRRLGGLEGA
                     TAGRSAEEELLEHLTSDEVKEALEALPEQFRMAVLLADVEGFAYKEIAEILDIPIGTV
                     MSRLHRGRKAMQKQLYEFGKQRNLVPHEVEPTPTAT"
     gene            2022..>2783
                     /locus_tag="VFP02_09015"
     CDS             2022..>2783
                     /locus_tag="VFP02_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543209.1"
                     /translation="MQTVYSDKHRLHHAHGELIDGALRPPHERPERADLILAAVRAAE
                     LGEVVEPTAHGIEPLARVHDEGFLAFLQEAWSLWLADHGDETDALPLAWPSRRMQQRE
                     PEAVDGRLSYFSFDAGSPIMAGTAEAVLASADVALTGADLVLGGERAAFSLCRPPGHH
                     AALDLYGGYCFVNNAAAAAQALIDGGLERIAVVDVDYHHGNGTQDIFWSRGDVLVTNL
                     HGDPRQEYPFFTGYADERGSEGGVDANRNYPLPMGT"
BASE COUNT          401 a         1004 c          956 g          422 t
ORIGIN      
        1 cgatggcgag gaccgtcagc acgagcactg gtgcgagcca gaaatgccag acgacaccga
       61 gaaccatggg cgcggagcat aacgggtacc ttctcccacc cagatgcgcc ggatcggggg
      121 gatcgccgtt gtcgtactcg tactggccgg ggcttgcggc ggcggcgaaa cggctgccac
      181 ccccgagccg acggtcacca cggtgacccc ggcgccgatg cgtacggagg tggaacgcct
      241 caccctcgac ctggtggacc cgtcccggcc cacccctgcc ggtgccggtc tgccccacgc
      301 cgacgaccgc cggctcgtga ccgacgtcta cgtgcccgcc ggccgcggcc cctttccctt
      361 catcgccttc agccacggcc tgaacgggca cccccgcaag ttcacccaac tcctcggaca
      421 gtgggccgaa gcgggctacg ccgtcgccgc gccggcgttc ccgctgtcca acgacgaggt
      481 gccgggcgtt tccaccttcg ccgacctgcc cagccagccc ggcgacgtca gcttcgtcat
      541 cgaccagctc ctggccatga gcgaggacga gagcgacgcc ctctacggcg tcgtcgaccc
      601 ggaccgcatc ggcgtggccg gcctgtccct cggcggggcc acgacctacg gcgtggcgtt
      661 caacgactgc tgcctcgacg agcggcccgt ggccgccatg gtcctggacg gcgcccgact
      721 ggccgtcggc ggcgagttcc acatggactc gggcctgccg ctgatgatca tccacgccga
      781 cgaggacttc gccctcccct acgccgaggc ggccgacgcc tacgtcgacg ccatcgcgcc
      841 gaagtacctg gtgaccctgc acgagttcgc ccacgcctcg ccctacgaga acgaccccga
      901 ccccgccgat gagctggtca cggccagcac catcgccttc tgggaccgct acctgaagga
      961 cgacgagagc gccgaccagc gcatcgtcga cgccatcacc ccgtccagtc tcgccaccct
     1021 cgaatccgag accggctgac gacggatctg gttgcacccc atcgcgccgg accgccggga
     1081 gacgcaacca gaacgactag ggggtggtgg gcgcggggtc ctcggagcag gcggcgacga
     1141 tgcgggcccg gaggtcgtcg gggacccggt cgatgcactt cgaggcgatg accttgcgca
     1201 gctcggactc gaagccggcg atgtcgccgc agggcgggca gtcgtcgagg tggaagcgga
     1261 tcttctcgcg ggcctcgtcg gtcagctcgc cgtcgaggta ggtgtagagc tcagcgaggg
     1321 tctcgtcgca cttgtcggac atgggctcag gtggcggtcg gggtcggttc gacctcgtgg
     1381 gggacgaggt tgcgctgctt gccgaactcg tacaactgct tctgcatggc ctttcttccc
     1441 cggtggagcc gactcatcac cgtgccgatc gggatgtcga ggatctcggc gatctccttg
     1501 taggcaaagc cctcgacgtc ggccagcagc acggccatgc ggaactgctc gggcagggcc
     1561 tccagggcct ccttcacctc gtcggaggtg aggtgctcga gcagctcctc ttcggcgctg
     1621 cgcccggccg tcgccccctc caacccgccg aggcggcggt agaggtagag gtcctcgacc
     1681 tcgtcgaggt ccgtctcgtc cggccggcgc ttcttggccc ggtaggtgtt gatgtaggtg
     1741 ttggtcagga tgcggtagag ccacgccttg aggttggtgc cctgctggaa ggtgtggaag
     1801 gcccggtagg ccttgaggta cgtctcctgg accaggtcct cggcgtcggc cgggttgcgg
     1861 gtcatgcgca gggccgcgcc gtagaggccg tccatgagcg gcatcgcctg ctcactgaag
     1921 gtggcctggt ccgccatggg cctgacccta gggggtgacc gtggtgggcg acacggcggt
     1981 cgggccggag tgggcgacgg cgccgtgcta cacccgggac cgtgcagacc gtctattccg
     2041 acaagcaccg gctccaccac gcccacgggg agctgatcga cggggccctt cggccgccgc
     2101 acgagcggcc ggaacgggcg gacctgatcc tggccgcggt gcgtgcggcc gagctgggcg
     2161 aggtcgtcga gccgacggcc cacggcatcg agcccctggc ccgggtccac gacgaggggt
     2221 tcctggcctt cctccaggag gcctggagcc tctggctggc cgatcatggt gacgagaccg
     2281 acgccctgcc tctggcctgg ccatcgcggc ggatgcagca gcgggagccc gaggccgtcg
     2341 acgggcgcct ctcctacttc tccttcgacg ccgggtcgcc gatcatggcc ggcaccgcgg
     2401 aggccgtcct ggcttccgcc gacgtggccc tcaccggcgc cgacctggtg ctgggcggcg
     2461 agagggcggc cttctcgctc tgccgtccgc cggggcacca cgccgccctc gacctctacg
     2521 gcggctactg cttcgtcaac aacgcggcgg ccgccgccca ggccctgatc gacggcggat
     2581 tggagcggat cgccgtggtc gacgtcgact accaccacgg caacggcacc caggacatct
     2641 tctggtcccg aggcgacgtg ctggtgacga acctccacgg cgatccccgc caggagtacc
     2701 ccttcttcac cggctacgcc gacgagcggg gcagcgaggg cggcgtcgac gccaaccgga
     2761 actacccgct gcccatgggc acc
//
LOCUS       DASTFV010000495         2685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1825518, whole genome shotgun sequence.
ACCESSION   DASTFV010000495 DASTFV010000000
VERSION     DASTFV010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1825518"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VFP02_09020"
     CDS             complement(<1..833)
                     /locus_tag="VFP02_09020"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9543210.1"
                     /translation="MAADVIDRLRSELGPDRIVTDADVLAPYETDWTGRFTGRARAVV
                     RPRSVDEVAAVIAACRQHGIALVPQGGNTGLVGGGVPRGGEVVLATAALAGPPTVDPV
                     AGQITVGAGRTLGEVQRAAAAHGWRYPIDLGARDTATIGGTVATNAGGNHVLAYGMTR
                     RHLLGIEAVLGTGAVVRHLGGLVKDNTGYDLAALLCGSEGTLGVVTELRLALVPPPEE
                     TAVALVGLDDLAAAVAVAAEARRHLPDLEAAEVVLADGVTLVRDHLSLAPVLDPEPPV
                     TL"
     gene            865..2439
                     /locus_tag="VFP02_09025"
     CDS             865..2439
                     /locus_tag="VFP02_09025"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET9543211.1"
                     /translation="MGTVPFTPTVHAPAGLVCSADHLASSAGLTMLSRGGSAVDAAIA
                     ANAVLAVADPHLCGMGGDLFALVHDGSGPPLALDAAGRAGRGADPDRLRGEGHAVMPF
                     RGDIRSVTIPGCVDGWCALHERFGRLPLADVLAPAITYAQDGYNASLLLAFAAPMVAA
                     VPGVEDWATRPVHDGQRLHRPRTAEALRAIVRQGRDGFYGGAFGEGLLTLGAGEYEPD
                     DLLPRARWVDPLALRAWGHDLWTMPPSSQGYLILAGAGVLDPVLLPEPTDAAWPHLLA
                     EAARLTGADRYAVLSDSAIGADLLAGARLDAVRDRLDPRHRVPTASGPVATGDTTVVT
                     AVDRDRQGVTLIQSNASDFGAHLVEPNTGTFLHNRGIGFRLEAGHSAEYGPGRQPPHT
                     LSPALVTTTGGELRAVVGTMGGDSQPQVVLQLLARSLAAGEAPGPAISAPRVVLTRSA
                     AGSGFDLWDGPDGLLVEDHAPVGWVEGLSERGHDLVVRNGYGAGFGHAHLIEVTDDGL
                     RGAADPRAVIGSAAGH"
     gene            complement(2462..>2685)
                     /locus_tag="VFP02_09030"
     CDS             complement(2462..>2685)
                     /locus_tag="VFP02_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9543212.1"
                     /translation="LFFGRHFRYVGRSGSMTEYRREDLDARGRLRNAGHQAAQLPWFS
                     RNVLIKVLIVAKLGRFTRLLGHAEPTWPF"
BASE COUNT          338 a         1007 c          983 g          357 t
ORIGIN      
        1 agggtgaccg gcggctccgg gtcaaggacc ggggccagcg agaggtggtc ccgcacgagg
       61 gtgaccccgt cggccaggac gacctcggcc gcctccagat cgggaaggtg gcgacgggcc
      121 tccgcggcca cggccacggc ggccgcgagg tcgtcgagcc cgaccagggc caccgcggtc
      181 tcctcggggg gtgggaccag ggccaggcgg agctcggtga ccacccccaa cgtcccttcg
      241 gatccgcaga gcagcgcggc caggtcgtag cccgtgttgt ccttcaccag cccgccgagg
      301 tggcggacca ctgcacccgt gcccaacacg gcctcgatcc ccaggaggtg gcgacgggtc
      361 atgccgtagg ccaggacgtg gttgcctccg gcgttggtgg ccacggtccc cccgatggtc
      421 gcggtgtccc gggcgccgag gtcgatcggg tagcgccacc cgtgggcggc agcggcccgc
      481 tggacctcac ccagcgtgcg gcccgccccg accgtgatct gcccggccac ggggtccacg
      541 gtgggtggtc cggccagcgc cgccgtggcc agcacgacct cgcccccgcg cggcacgcct
      601 ccgccgacca gcccggtgtt gcccccctgg ggcacgaggg cgatgccgtg ctgccggcag
      661 gcggcgatga cggcggcgac ctcgtcgaca ctccggggac ggaccaccgc ccgggctcgc
      721 ccggtgaagc gacccgtcca gtccgtctcg tacggggcga gaacgtcggc atcggtgacg
      781 atccggtccg ggccgagctc gctacgcaga cggtcgatca cgtcggcggc catggtcgca
      841 tggtggcacc actacggtgc cgcgatgggc acggtcccct tcacgcccac cgtgcacgcg
      901 cccgctggcc tggtctgctc ggccgatcac ctggcctcgt cggccggact gaccatgctc
      961 tcccgaggag ggtcggcggt cgacgccgcc atcgccgcca acgcggtgct cgccgtggcc
     1021 gaccctcatc tctgcgggat gggcggtgac ctcttcgccc tcgtccacga cggatccggg
     1081 ccgccgctgg cgttggacgc cgcgggacgg gccggccgcg gcgcggaccc tgatcgcttg
     1141 cggggcgagg gccatgcggt gatgcccttc cggggggaca tccgctccgt caccattccc
     1201 gggtgtgtcg acggatggtg tgccttgcac gagcgcttcg gtcgcctccc gctggccgac
     1261 gtcctcgccc ccgccatcac ctacgcccaa gacggctaca acgcctcgct gctcctggcc
     1321 ttcgccgccc ccatggtcgc ggcggtgcct ggggtggagg actgggcgac gcgtccggtg
     1381 cacgacggcc agcgtctgca ccggcctcgg acggccgagg ccctgcgggc gatcgtccgg
     1441 cagggccggg acgggttcta cggcggggcc ttcggtgagg gcctcctgac cctcggcgcg
     1501 ggtgagtacg agcccgacga cctcctgccc cgggcacggt gggtcgaccc cctggccctg
     1561 cgggcgtggg gccacgacct ctggacgatg ccgccgagct cgcagggcta cctcatcctg
     1621 gccggggccg gggtcctcga ccccgtgctg ctccccgagc ccaccgacgc cgcctggccc
     1681 catctgttgg ccgaggcggc ccgactgacc ggtgcggacc gctacgcggt gctcagcgac
     1741 tcagccatcg gggcggatct cctcgccggc gcccgcctgg acgccgtgcg ggatcgactc
     1801 gatcccaggc accgggtgcc caccgccagc ggcccggtcg ccaccgggga cacgacggtg
     1861 gtgaccgcgg tggaccggga ccgccagggg gtcacgctga tccagtccaa cgcctcggac
     1921 ttcggggccc acctcgtcga accgaacacg ggcaccttcc tgcacaaccg gggcatcggg
     1981 ttccgcctcg aggccgggca ctcggctgaa tacgggcccg gtcgtcagcc tccacacacc
     2041 ctcagccccg ccctcgtgac cacgaccggg ggcgagctcc gggccgtggt cggcacgatg
     2101 ggaggcgaca gccagcccca ggtggtgctc cagctcctgg cccgctcact ggcggccggg
     2161 gaggctcccg gcccggcgat ctcggctccc cgggtggtcc tgacccggtc ggcggctggg
     2221 tccggcttcg acctgtggga cgggccggac ggtctcctcg tcgaagatca cgctcccgtg
     2281 ggctgggtcg aaggcctgag cgaacggggc catgacctgg tggtgcgaaa cggctacgga
     2341 gccgggttcg gccacgccca cctcatcgag gtcaccgacg acggactgcg gggcgccgcg
     2401 gatccccggg ccgtgatcgg ttcagccgcg ggtcactgac ggcgcgcagc cgacgccggg
     2461 ctcagaaggg ccaggtcggc tcggcgtggc ccagcaggcg ggtgaaccga cccagcttgg
     2521 ccacgatcag caccttgatc aggacgttgc gggagaacca cgggagctgg gccgcctggt
     2581 gcccggcatt gcgcagccgc ccgcgggcgt cgaggtcctc tcgccggtac tccgtcatcg
     2641 agccggagcg gccgacgtag cggaagtggc ggccgaagaa gagca
//
LOCUS       DASTFV010000496         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1827394, whole genome shotgun sequence.
ACCESSION   DASTFV010000496 DASTFV010000000
VERSION     DASTFV010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827394"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VFP02_09035"
     CDS             complement(<1..512)
                     /locus_tag="VFP02_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543213.1"
                     /translation="MLVDSPCVACDGDGGPVCPACAATLVPVGAVDVDGLDGAWALMA
                     YAGPARALIRGLKYTNRRAALGVLATAAARLVDEGVAVVTWVPASPAHHRERGYDQGR
                     LLAGRVARALGRPNRNLLVRSGERAQPGLDRSDRLTGPTLGPRRQVTGPVLVVDDVLT
                     TGGSLAAAARA"
     gene            565..1197
                     /locus_tag="VFP02_09040"
     CDS             565..1197
                     /locus_tag="VFP02_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET9543214.1"
                     /translation="MRFIQTPPAIEVRRRLPRLLVGLVICGIGLAFIIRADLGLDSWD
                     VLHQGLSKQTGISIGLVTILVGFALFAISLPLGEQIGLGTLLNVLLIGTTIDLTLWVL
                     DEPSSAAVQWLFLIGGTVAFAVGSGFYIGAGLGPGPRDSVMTSLARRGLRVGVVRTGI
                     ELTVLLLGWTLGGSVGVGTLLFALTIGPLVAFFLPRLSLEPVGPTTLEAY"
     gene            complement(1194..1637)
                     /locus_tag="VFP02_09045"
     CDS             complement(1194..1637)
                     /locus_tag="VFP02_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543215.1"
                     /translation="MTEIEDGPVGRAGWAWIAVLCGYSLVRAVLAWPLLVHYDIDPAT
                     FLVLDVGTAYPLGLAQVRIVQGFAARDFAAVQAWGAVAGISFITPYAYLLVTGHQALP
                     VYAMAGLVGVVALIATASVLRLRQACLHSDCLPVAERLEPVPVDV"
     gene            complement(1685..>2600)
                     /gene="mtnA"
                     /locus_tag="VFP02_09050"
     CDS             complement(1685..>2600)
                     /gene="mtnA"
                     /locus_tag="VFP02_09050"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HET9543216.1"
                     /translation="GDRVRLIDQRLLPGRLEVVEATTVPDLCALITTLAVRGAPALGA
                     AGAMGVALAHVRGEDTAAAADVLIATRPTAANLAWGARRALAADDPVGEAVTIATDDV
                     ECCRAIGRNGVALVPQEGRVLTHCNAGGLACVGYGTAVGVIRAAHEAGRRPRVWVDET
                     RPVLQGSRLTAWELGRLGIEHTLVVDAVAGSLMAEGLVDLVVVGADRIAANGDVANKI
                     GTYPLAVLAQHHGVPFYVAAPRSTFDAGCPDGSAIQVERRDADEVAMVGGVRLAPEGV
                     NVENRAFDVTPARLVTSYVMEDGVHARP"
BASE COUNT          363 a          949 c          940 g          348 t
ORIGIN      
        1 gctcgggccg cggcggccag cgagccgccc gtggtgagga cgtcgtccac gacgaggacc
       61 ggtcccgtca cctgccgccg cgggccgagg gtcggcccgg tcaggcggtc cgaccggtcg
      121 aggccgggtt gggcccgttc tcccgaccgg accagcaggt tgcggttcgg tcgtcccaac
      181 gctcgggcca ctcggccggc cagcaaccga ccttggtcgt acccccgttc gcggtgatgg
      241 gccggactgg ccgggaccca ggtcacgacc gccactcctt cgtcgaccaa tcgggcggcg
      301 gccgtcgcca ggaccccgag cgcggcccgc cggttcgtgt acttgaggcc gcggatcagc
      361 gcccgggccg ggccggcata ggccatgagc gcccaggccc cgtccagccc gtcgacgtcc
      421 accgcgccaa cgggcaccag ggtcgcggca caggcgggac agaccggtcc gccgtcaccg
      481 tcgcaggcca cacagggcga atccacgagc acggaggaaa cctacggatg gggggtgaca
      541 ggccagcggt accgtcctcc gatcgtgcgc ttcatccaga cccctcccgc gatcgaggtg
      601 cgtcggcgcc tcccccggct gctcgtgggc ctggtgatct gcggcatcgg gctggccttc
      661 atcatccggg ccgacctcgg cctcgactcc tgggacgtgt tgcaccaggg tctgagcaag
      721 cagaccggca tctcgatcgg gctggtcacg atcctggtcg gcttcgccct gttcgccatc
      781 tcgctccccc tgggcgagca gatcggcctg ggcacgctgc tcaacgtgct cctcatcggc
      841 acgaccatcg acctgaccct ctgggtcctc gacgagccgt cgtccgccgc ggtgcagtgg
      901 ctcttcctga tcggcgggac cgtggccttc gccgtcggct ccggcttcta catcggcgcc
      961 gggctcgggc ccgggccgcg ggacagcgtg atgacctcgt tggcccgccg aggcctgcgg
     1021 gtgggcgtgg tccgcaccgg gatcgagctc accgtcctgc tcctggggtg gaccctcggc
     1081 ggctcggtcg gcgtgggcac gctgctcttc gccctcacca tcggcccact ggtcgcgttc
     1141 ttcctgcccc ggctgagcct ggagccggtg ggtcccacga cgctcgaggc gtactagacg
     1201 tcgacgggaa ccggttcgag gcgttcggcg accgggaggc agtcgctgtg gaggcaggcc
     1261 tggcggaggc gcaggaccga ggcggtggcg atcagggcca ccaccccgac cagaccggcc
     1321 atcgcgtaga caggcagcgc ctggtggccg gtcaccaaca ggtaggcgta gggggtgatg
     1381 aaggagatcc cggccacggc gccccaggcc tgcacggctg cgaagtcccg cgccgcgaag
     1441 ccctggacga tgcggacctg agccaggccc agggggtacg cggtgccgac gtccaacacc
     1501 aggaaggtgg cggggtcgat gtcgtagtgg accagcaagg gccaggccag cacggcccgg
     1561 acgagggagt acccgcagag gaccgcgatc caggcccagc ccgctcggcc aacgggcccg
     1621 tcttcgatct cggtcacgac gcggtcaggg taggccgagg gtcctcccga gggacggcat
     1681 gccctcacgg gcgggcgtgg acgccgtcct ccatcacgta gctcgtcacc agtcgggccg
     1741 gggtcacgtc gaacgcccgg ttctccacat tcaccccctc gggcgccagc cgcacacctc
     1801 ccaccatggc gacctcgtcg gcgtctcggc gctcgacctg aatggcggat ccgtccgggc
     1861 agccggcgtc gaaggtggag cgaggggcgg ccacgtagaa cggcacgccg tggtgctggg
     1921 cgaggacggc cagcggatag gtcccgatct tgttggccac gtcgccgttg gcggcgattc
     1981 gatcggcgcc cacgaccacg aggtcgacga gcccctcggc catgagcgag ccggccaccg
     2041 cgtccacgac cagggtgtgt tcgatgccca ggcgccccaa ctcccaggcg gtcagccggg
     2101 acccctgcaa caccgggcgg gtctcgtcga cccagacccg agggcgacgc ccggcctcgt
     2161 gggcggcgcg gatgacgccg accgcggtgc cgtagcccac acaggccagg ccaccggcgt
     2221 tgcaatgggt caggacgcgg ccttcctggg ggaccagggc gacaccgttc cggccgatgg
     2281 cacgacagca ctcgacgtcg tcggtggcga tggtcacggc ctccccgacc ggatcgtcgg
     2341 cggccagggc ccggcgggcg ccccaggcca ggttcgccgc cgttggccgg gtggcgatga
     2401 gcacgtcggc ggcggcggcg gtgtcctcgc cccgcacatg ggccagcgcc acacccatcg
     2461 ccccggccgc gccgagagcc ggagcgcccc gcacggccag ggtcgtgatc agggcacaca
     2521 ggtcgggaac ggtcgtggcc tcgaccacct cgagtcgacc gggcaggagg cgttggtcga
     2581 tgaggcggac ccgatcgccc
//
LOCUS       DASTFV010000497         4197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1833348, whole genome shotgun sequence.
ACCESSION   DASTFV010000497 DASTFV010000000
VERSION     DASTFV010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833348"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VFP02_09055"
     CDS             <1..280
                     /locus_tag="VFP02_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET9543217.1"
                     /translation="DQGRLLAGRVARALGRPSRNLLVRSGARAQTGLDRSDRLAGPTL
                     RPRRQVTGPVLVVDDVLTTGGSLAAAARALRRAGATRVLGVVLAATPG"
     gene            312..1007
                     /locus_tag="VFP02_09060"
     CDS             312..1007
                     /locus_tag="VFP02_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9543218.1"
                     /translation="MEPLLTDSDLFFGLDDLPDDPVRIILGDENGLYRRGLLTALEDE
                     NDLEVVAEAGPGADLIAAVRALAPDVVVCSLSMGSVGGVETTFAITEVMPLVRIAVLS
                     TDQDPPSKTLAAVRSGALGEIDKVVALEEAARVIRGLHGGWPVFGPATWAGIAIEAPS
                     WLAKGDTVKDGERAALGALAAGGTWSDAADAVGVRAEAARNQVRNLLARCRAAGYPDA
                     ADAALGAGLVAAP"
     gene            1043..3763
                     /gene="secA"
                     /locus_tag="VFP02_09065"
     CDS             1043..3763
                     /gene="secA"
                     /locus_tag="VFP02_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET9543219.1"
                     /translation="MGALDRLLRAGEGKKVRAIAALVPDVNALEPELAALSDDALKAK
                     TGEFKGRLERGEEIDDLLIEAFAVTREAARRVIGQRHYDVQVMGGAALHFGWVAEMKT
                     GEGKTLVSTLPVYLNALAGKGVHLVTVNDYLATRDSEWMGQVHKFLGLSVGLVVPGQN
                     DPDHKREQYAADITYGTNNEYGFDYLRDNMALSRERQVQRGHHYAIVDEVDSILVDEA
                     RTPLIISGRMADAAKLYYQFANIVKTLQRDVDYEVDEEKRTVAPEESGIEKVEAQLGI
                     ENLYDEVSANLVHQLQAALRAKELFKRDKDYIIQRGEVKIVDEFTGRILEGRRWSEGL
                     HQAVEAKEGVKIKEENQTLATITLQNYFRLYDKLAGMTGTATTEAAELASTYALDVVP
                     IPTNLPTQRLDQADLIYKSEDAKFDAVVDDLAERYEAGQPCLVGTVSVETSERLSRAL
                     EKRGVRHEVLNAKQHTREAEIVAQAGRLHSVTVATNMAGRGVDIILGGNHESLAMRDV
                     RAEELDPDDEEGAARLEELTAKYKAETTAEGERVKELGGLYVLGTERHESRRIDNQLR
                     GRSGRQGDPGESRFYLSLEDELMRLFATGAMDWVMNKSLPDDVPIESKMVSKAIERAQ
                     TTVEQRNAEIRKNVLKYDEVMSEQRRVIYARRSQILDGADLRTEAMEYLAEAVDSAIS
                     TYCVSDFAEEWDLDGLAGEVRTFWPSALEAADLEGAEGTDALYDRLMAEAVAHYEARE
                     AEVGPDTMRKLEREVMLRIIDQKWREHLADMDYLRDGINLRAMGQKDPLNEWQREGYE
                     FFGSMMAAIAQDFVRYLMHIQVTVQPAERRPEPALSDVQTSGPEDPVQGATGMARAAM
                     GPAGDNAAAAPQAVEEPVNEPMVKSEWDKTGRNEPCPCGSGKKYKLCHGR"
     gene            3779..>4197
                     /locus_tag="VFP02_09070"
     CDS             3779..>4197
                     /locus_tag="VFP02_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153759895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HET9543220.1"
                     /translation="MKDFSEDVAALRGRLAAAAGYLRVDELRAHRPQLETEASRPDLW
                     DDPDKARAVTGELAAVVDDLDLYDRLTTEVDDVATLAEMAREEKDESLEPEIEEAMTA
                     LVATFSELELRALFQGEYDERDAVVEVHSGAGGTDSQ"
BASE COUNT          699 a         1466 c         1474 g          558 t
ORIGIN      
        1 cgaccaaggt cggttgctgg ccggccgagt ggcccgggcg ttgggacgac cgagccgcaa
       61 cctgctggtc cggtcggggg cacgggccca aaccggcctc gaccggtcgg accgcctggc
      121 cgggccgacc ctgcgcccgc ggcggcaggt gacggggccg gtcctcgtcg tggacgacgt
      181 cctgaccacg ggcggctcgc tggccgccgc ggcccgagcc ttgcggcggg ccggggccac
      241 ccgcgtgctc ggggtcgtcc tcgccgccac cccgggttga gagttccctt cgatgtgccg
      301 atggaccacg gatggaaccc ctcctgacgg actccgacct cttcttcggc ttggacgacc
      361 tgcccgacga tcccgtgcgg atcatcctcg gcgacgagaa cggcctctac cgccgcggcc
      421 tgctcacggc gctggaggac gagaacgacc tcgaggtggt ggccgaggcc gggcccggcg
      481 ccgacctgat cgccgcggtc cgggcccttg cccctgacgt cgtcgtgtgc tcgctcagca
      541 tgggcagcgt cggcggggtc gagaccacct tcgccatcac cgaggtgatg cccctcgtcc
      601 gcatcgccgt gctcagcacg gaccaggacc caccttccaa gacgctggcc gcggtccgat
      661 ccggcgccct cggcgagatc gacaaggtcg tggccctgga agaggcggcc cgcgtcatcc
      721 gcgggctcca cggaggttgg ccggtgttcg gcccggccac ctgggccggc atcgccatcg
      781 aggccccgag ttggctggcc aagggcgaca ccgtcaagga cggtgagcgg gcggcgctgg
      841 gggcgttggc cgccggcggc acgtggtcgg acgcggccga cgcggtcggg gtccgggccg
      901 aggccgctcg caatcaggtc cgcaacctcc tggcccgctg ccgggccgcg ggctatccgg
      961 acgcggccga cgccgcgctc ggtgccgggc tggtcgccgc gccctgaccg acgcgcgaca
     1021 gcgccggtag cctccatcaa ccgtgggtgc tctcgatcgt ctcctccgcg ccggtgaagg
     1081 caagaaggtc cgtgccatcg ccgcgctcgt tcccgacgtc aacgcgctcg agcccgagct
     1141 ggcggcgctc agcgacgatg cgctgaaggc caagaccggc gagttcaagg gtcgcctcga
     1201 gcggggcgag gagatcgacg acctgctcat cgaggccttc gcggtgaccc gtgaagccgc
     1261 ccggcgggtg atcggccagc gtcactacga cgtccaggtc atgggcgggg cggccctgca
     1321 cttcggctgg gtggccgaga tgaagaccgg tgagggcaag accctggtct cgaccctgcc
     1381 cgtctatctc aacgcgctgg ccggaaaggg cgtgcacctg gtcacggtca acgactacct
     1441 ggccacccgt gactcggagt ggatgggcca ggtccacaag ttcctcggcc tgagcgtcgg
     1501 tctcgtcgtc cccggccaga acgaccccga ccacaagcgc gagcagtacg ccgccgacat
     1561 cacctacggc accaacaacg agtacggctt cgactacctg cgggacaaca tggcgctgtc
     1621 ccgggagcgg caggtccagc ggggccacca ctacgccatc gtcgacgagg tcgactcgat
     1681 cctggtggac gaggcccgta cgccgctgat catcagcggg cgcatggccg acgcggccaa
     1741 gctctactac cagttcgcca acatcgtgaa gaccctgcag cgtgacgtcg actacgaggt
     1801 cgacgaggag aagcgcacgg tcgccccgga ggagtccggc atcgagaagg tcgaggccca
     1861 gctcggcatc gagaacctct acgacgaggt ctcggccaac ctcgtccacc agctccaggc
     1921 cgcgctgcgg gccaaagagc tcttcaagcg ggacaaggac tacatcatcc agcggggcga
     1981 ggtgaagatc gtcgacgagt tcaccggccg catcctcgag ggccggcgct ggtccgaggg
     2041 cctccaccag gccgtcgagg ccaaggaggg ggtgaagatc aaggaggaga accagaccct
     2101 ggccaccatc accctccaga actacttccg cctctacgac aagctcgccg ggatgaccgg
     2161 tacggccacc accgaggccg ccgagctggc gtcgacctac gccctcgacg tcgtgccgat
     2221 ccccaccaac ctgccgaccc agcgcctcga ccaggccgac ctgatctaca agtccgagga
     2281 cgccaagttc gacgccgtgg tcgacgacct ggccgagcgc tacgaagccg gccagccctg
     2341 cctggtgggc acggtgtcgg tcgagacgtc cgagcggctc tcgcgggcgc tcgagaagcg
     2401 gggcgtgcgc cacgaggtcc tgaatgccaa gcagcacacc cgggaggccg agatcgtggc
     2461 ccaggccggg cgcctgcact cggtcacggt ggccacgaac atggccggtc gaggtgtcga
     2521 catcatcctc ggtggcaacc acgagtcgtt ggccatgcgc gatgtgcggg ccgaggagct
     2581 cgacccggac gacgaggagg gcgccgcccg cctcgaggag ctcaccgcca agtacaaggc
     2641 cgagaccacc gccgagggtg agcgggtcaa ggagctgggc ggcctgtacg tgctcggcac
     2701 cgagcgccac gagagccgcc ggatcgacaa ccagctccgc ggccgttccg gtcgccaggg
     2761 cgaccccggg gagagccgct tctacctgtc cctcgaggac gagctgatgc gcctcttcgc
     2821 cacgggggcc atggactggg tgatgaacaa gtcgttgccc gacgacgtgc ccatcgagtc
     2881 caagatggtc tccaaggcca tcgagcgggc ccagaccacc gttgagcagc gcaacgccga
     2941 gatccgcaag aacgtcctca agtacgacga ggtcatgagc gagcagcggc gggtcatcta
     3001 cgcccggcgc tcccagatcc tcgacggcgc cgatctgcgc accgaagcca tggagtacct
     3061 ggccgaggcc gtcgactccg ccatctcgac ctactgcgtc tcggatttcg cggaggagtg
     3121 ggacctggac ggcttggccg gcgaggtccg cacgttctgg cccagcgccc tcgaagccgc
     3181 cgacctcgag ggagccgaag gcaccgacgc cctctacgac cggctgatgg ccgaggcggt
     3241 cgcccactac gaggctcgcg aggccgaagt cggcccggac accatgcgca agctcgagcg
     3301 cgaggtgatg ctgcgcatca tcgaccagaa gtggcgcgag cacctggccg acatggacta
     3361 cctgcgggac ggcatcaacc tgcgggcgat gggtcagaag gatccgctca acgaatggca
     3421 gcgggagggc tatgagttct tcggatcgat gatggcggcc atcgcccagg acttcgtccg
     3481 gtacctcatg cacatccagg tcacggtgca gcctgccgag cggcgcccgg agccggcgct
     3541 gagcgacgtg cagacctcgg ggcccgagga cccggtccag ggggccacgg gcatggcccg
     3601 ggcggccatg ggcccggcgg gtgacaacgc cgcggcggcc ccacaggccg tcgaggagcc
     3661 ggtcaacgag ccgatggtca agagcgagtg ggacaagacg ggtcggaacg agccctgccc
     3721 ctgtggctcg ggcaagaagt acaagctctg ccacggccgc taggccgacc gtcaccgcat
     3781 gaaggacttc tccgaggacg tcgctgccct acgaggtcgc ctcgccgccg cggcgggcta
     3841 cctgcgggtg gacgagctgc gggcccatcg cccccagttg gagacggagg cctcgcgtcc
     3901 ggacctctgg gacgacccgg acaaggcccg tgcggtcacc ggcgagctgg cggccgtggt
     3961 cgatgacctc gacctctacg accggttgac gacggaggtg gacgacgtcg ccacgctggc
     4021 cgagatggcc cgggaggaga aggacgagtc tctcgagccc gagatcgagg aggcgatgac
     4081 ggccttggtg gcgaccttct ccgagctcga gctgcgggcc ctgttccagg gggagtacga
     4141 cgagcgcgac gccgtcgtcg aggtccactc cggtgctggc ggcaccgact cccagga
//
LOCUS       DASTFV010000498         3701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1835104, whole genome shotgun sequence.
ACCESSION   DASTFV010000498 DASTFV010000000
VERSION     DASTFV010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835104"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VFP02_09075"
     CDS             <1..552
                     /locus_tag="VFP02_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET9543221.1"
                     /translation="DGDRVVELVFGPYHEDTDHPWHQLERGELTFEDCNAALAAAAAA
                     EGVTLDPIALLMRMGEGGTARIVRENVLAAAQRVKADGYRTAIITNNIREFREGWGSL
                     FDVESVAELVIDSSEVGVRKPDPAIFRLALERLGGIAPERAVFLDDAAGNIAGAAALG
                     IHAILVEDDPEGALAELDAALAG"
     gene            complement(616..1860)
                     /locus_tag="VFP02_09080"
     CDS             complement(616..1860)
                     /locus_tag="VFP02_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9543222.1"
                     /translation="MTDAVDVPPVFFNPLDPGYLADPYPHLTEMREADPVHESPLGIW
                     VLFRYDDVFNLLRNPTMSVEDANISGGLNEMREQMFEAMLAEFDDDARGRGDRAILNL
                     DPPDHTRIRKLVAKAFTMRRVEQLRENVQVQVDAVLDRVAGGGTWDVVEELAFPLPFQ
                     VISDLLGMPDGDRDQIRAWSHALTKTLDPIVAEEDLRAAFEASRAMSDHLEDVVAWKR
                     EHPADDVLTGLVQAEEDGDRLSAEELRDQIVLIFIAGHETTVNLIGNGMQALLRHPDQ
                     LARWRDDPALDATAVDELLRYDAPVQLSRRIVMSEFELEGRSIAPGTLVMTHLAAANR
                     DPAKWGPTADRLDVGRADAGQHVTFGSGIHFCLGASLARLEGQVAMSSILRRFPDLEQ
                     AGDAVPNGRITLRGLDSLPVTS"
     gene            1891..2673
                     /locus_tag="VFP02_09085"
     CDS             1891..2673
                     /locus_tag="VFP02_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET9543223.1"
                     /translation="MADAPACDPALLRQAVELARRAGDFTLPYFRSLELAVEHKHDDT
                     PVTEADRGAERLLRDLLADAFPADAVIGEEEDDTAGTSGRTWVIDPIDGTKAFTHGVP
                     LYSTLLAALDEHGPLLGVICLPALPETVWAGRGLGCFLDGEPCHVSEHDTVEGAYVTA
                     SALRNWPAGALDATHGAGAFVRTWGDAYGYALVATGRVEAMVDHQAAIWDIAPMPVII
                     GEAGGRMSDLAGADSITSDSGLATNGLLHDDLLALLAPGDVG"
     gene            complement(2670..3395)
                     /locus_tag="VFP02_09090"
     CDS             complement(2670..3395)
                     /locus_tag="VFP02_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HET9543224.1"
                     /translation="MTAPEPTTVDYADLSALFINCTLKRSPEVSNTAGLAEVSMDILE
                     ANGVKTEMIRALDHDIAAGVWPDMTEHGWDRDDWPPIFDKVMAADILVLCTPIWLGEK
                     SSVCTQVIERLYGNSHLLNEAGQYRYYGRVAGCLVTGNEDGVKHCAMNVLYSLQHLGF
                     TIPPQADAGWIGEAGPGPSYLDPGSGGPENDFTNRNSTFMTWNLMHIARMLKDQGGIP
                     VHGNQRSEWDAGCRFDFPNPEHR"
     gene            complement(3426..>3701)
                     /locus_tag="VFP02_09095"
     CDS             complement(3426..>3701)
                     /locus_tag="VFP02_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543225.1"
                     /translation="VILCAHFVHDEPVEVQLWPEHFDLATSFAEVNYGLSPGDAGHPW
                     PYFYLGPWSPPPVGGFWNEPFGASLEVQEVPTVDEVLAFFGTGRGSL"
BASE COUNT          547 a         1274 c         1328 g          552 t
ORIGIN      
        1 gacggtgacc gggtggtcga gctggtcttc gggccgtacc acgaggacac ggatcaccct
       61 tggcaccagc tcgagcgcgg ggaactgacc ttcgaggact gcaacgccgc cctcgccgcc
      121 gcggccgcgg ccgagggggt gacgctcgac ccgattgccc tgctcatgcg catgggcgag
      181 ggcggcacgg cccgcatcgt gcgcgagaac gtcctcgccg cggcccaacg ggtgaaagcc
      241 gacggctacc gcaccgcgat catcaccaac aacatccggg agttccgcga gggctggggc
      301 agcctgttcg acgtcgagtc ggtcgccgag ctcgtcatcg actcgtccga ggtcggcgtg
      361 cgcaagcccg acccggcgat cttccggctg gccctggagc gcctcggtgg catcgccccc
      421 gagcgggccg tcttcctgga cgacgccgcc ggcaacatcg cgggcgccgc cgccctcggc
      481 atccacgcca tactcgtcga ggacgacccc gagggcgctc tggcggaact ggacgccgcg
      541 ctcgcgggct gaccaccccg aacctgcgcc ggttacaccc cggatccgtg gagcgaggag
      601 cgcaagttcg agggttcagc tggtgacggg tagcgagtcc aggccgcgca gcgtgatgcg
      661 gccgttgggg acggcgtcgc ccgcctgctc gaggtccggg aagcggcgca ggatcgagct
      721 catggccacc tgcccctcga ggcgagccag cgaggcgccc aggcagaagt ggatgcccga
      781 gccgaaggtc acgtgctggc cggcgtcagc ccgacccacg tcgagtcggt cggcggtcgg
      841 gccccatttg gccgggtccc ggttggccgc ggcgaggtgg gtcatgacca acgtgcctgg
      901 ggcgatcgag cggccctcca gctcgaactc gctcatgacg atgcgtcggg acagctgcac
      961 cggggcgtcg tagcgcaaca gctcatcgac cgccgtggcg tccagcgccg ggtcgtcccg
     1021 ccagcgggcc agctggtcgg gatggcggag cagggcctgc atcccgttgc cgatgaggtt
     1081 caccgtggtc tcgtggccgg cgatgaagat gaggacgatc tggtcccgca gctcctcggc
     1141 actgagccgg tcaccgtcct cctcggcctg gaccagaccg gtcagcacgt cgtccgccgg
     1201 gtgctcccgc ttccacgcga ccacgtcctc gaggtggtcg ctcatcgccc ggctggcctc
     1261 gaaggcggcc cgcaggtcct cctcggccac gatcgggtcg agggtcttgg tcagggcatg
     1321 ggaccaggcc cggatctggt cccggtcgcc gtcgggcatg cccaacaggt cggagatgac
     1381 ctggaacggc agcgggaagg ccagctcctc gaccacgtcc caggtcccgc caccggcgac
     1441 ccgatcgagg acggcgtcca cctgcacctg cacgttctcc cgcagctgct cgacccgccg
     1501 catggtgaaa gccttggcga cgagcttccg gatgcgggtg tggtcgggcg ggtccaggtt
     1561 gaggatggcc cggtcgccgc ggccccgagc gtcgtcgtcg aattcagcca gcatcgcctc
     1621 gaacatctgc tcgcgcatct cgttgaggcc gccggagatg ttggcgtcct ccaccgacat
     1681 cgtggggttg cgcaggaggt tgaagacgtc gtcgtagcgg aacagcaccc agatgcccaa
     1741 cgggctctcg tgaacggggt ccgcctcgcg catctcggtc aggtgcgggt acggatcggc
     1801 caggtacccg gggtcgagcg gattgaagaa gacgggcggg acgtcgacgg cgtcggtcac
     1861 ccccgaaacc gtacgccgta cgatcgctcg atggccgatg ctcccgcctg tgaccccgcc
     1921 ctcttgaggc aggcggtcga gctcgctcgg cgggcgggcg acttcacgct cccctacttc
     1981 cgctcgctcg agctggcggt cgagcacaag cacgacgaca cccccgtcac cgaggccgac
     2041 cggggcgccg agcgcctcct gcgggacctg ttggccgacg ccttccccgc cgatgccgtc
     2101 atcggcgagg aggaggacga caccgcgggc acctcgggac ggacctgggt gatcgatccc
     2161 atcgacggca ccaaggcctt cacccacggc gtccccctct acagcacgct gttggccgcg
     2221 ctcgacgagc acgggcccct cctcggcgtc atctgcctgc ccgccctgcc cgagacggtg
     2281 tgggccggtc ggggcctcgg ctgcttcctc gacggcgagc cgtgccacgt cagcgagcac
     2341 gacaccgtcg agggggccta cgtcaccgcc agcgccctgc ggaactggcc cgccggcgcg
     2401 ctcgacgcca cgcacggggc gggggccttc gtgcgcacgt ggggcgatgc ctacggctac
     2461 gccctggtcg ccaccgggcg ggtcgaggcc atggtcgacc accaggccgc gatctgggac
     2521 atcgccccga tgcccgtgat catcggcgag gccggcggcc ggatgtcgga cctggccggt
     2581 gccgacagca tcacgtccga cagcgggctg gccaccaacg gcctcctcca cgacgacctg
     2641 ttggcgctgc tcgcgccggg cgacgtcggc tagcggtgct cggggttcgg gaagtcgaat
     2701 cggcagccgg cgtcccactc ggagcgctgg ttgccgtgca cggggatacc gccctggtcc
     2761 ttcagcatgc gggcgatgtg catgaggttc caggtcatga acgtggagtt ccgattggtg
     2821 aagtcgttct cgggaccgcc ggagcccgga tcgaggtagg acggcccggg gccggcctca
     2881 ccgatccagc cggcatcggc ctggggcggg atcgtgaacc cgaggtgctg aagggagtag
     2941 aggacgttca tggcgcagtg cttcaccccg tcctcgttgc ccgtgaccag gcagccggcg
     3001 acccggccgt agtaccggta ctggcccgcc tcgttgagca ggtggctgtt gccgtagagg
     3061 cgctcgatga cctgggtgca gaccgaggac ttctcgccca gccagatggg cgtgcagagg
     3121 acgaggatgt cggcggccat gaccttgtcg aagatcggcg gccaatcgtc cctgtcccac
     3181 ccgtgctcgg tcatgtcggg ccacacgccc gcggcgatgt cgtggtcgag ggcccggatc
     3241 atctcggtct tgaccccgtt cgcctcgagg atgtccatcg agacctcggc caggcccgcg
     3301 gtgttggaca cctcgggcga gcgcttcagc gtgcagttga tgaagagggc actcaggtcg
     3361 gcgtagtcga cggtggtggg ctcaggagcg gtcatgacgg tgctgtctgc cgacggggcg
     3421 aggcgttaca gcgatcctcg gccggtgccg aagaaggcga gcacctcgtc gacggtgggg
     3481 acctcctgga cctcgaggct ggcgccgaac ggttcgttcc agaaaccgcc caccggcggc
     3541 ggtgaccagg gcccgaggta gaagtacggc cagggatggc cggcgtcgcc cggcgagagg
     3601 ccgtagttga cctcggcgaa cgaggtggcc aggtcgaagt gctccggcca gagctggacc
     3661 tcgaccggct cgtcgtggac gaagtgggca cagaggatga c
//
LOCUS       DASTFV010000499         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1836305, whole genome shotgun sequence.
ACCESSION   DASTFV010000499 DASTFV010000000
VERSION     DASTFV010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836305"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..149
                     /locus_tag="VFP02_09100"
     CDS             12..149
                     /locus_tag="VFP02_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9543226.1"
                     /translation="MATASKMGRIASPDEMVGPVLFLVSDASSYMTGQVLVIDGGLAA
                     Y"
     gene            269..946
                     /locus_tag="VFP02_09105"
     CDS             269..946
                     /locus_tag="VFP02_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543227.1"
                     /translation="MSTLTKEPTVVLNGDSGDTRVTGADAPIKITRGRIDHLLIWSGG
                     AIAVVLVVAGVLLTWGSSFSEDYVHDELAAQAITFPPAAALEEEGRDDLVTYADQQVD
                     TGAEAEAYASFIAGHVEGIADGATYAELGDVEREAEAATVAALESGAPAAEVTALEEE
                     AAGISGQRDSIFRGEMLRGALLSTFAWSTIGQIAGYAAIAAFIGALVMAGLVIAGVLH
                     LSKTAHA"
     gene            complement(1008..1508)
                     /locus_tag="VFP02_09110"
     CDS             complement(1008..1508)
                     /locus_tag="VFP02_09110"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9543228.1"
                     /translation="MSEVLTTGRLRLTPLTVSDAEALLAAYADPAMFEFTGGSVPTLD
                     QLRDRLRSQTDATPEGTDWLNWVVRHDGVTIGVVQATVKGSWAVAEVAWEIGVPWQGH
                     GYASEAAGAVVDWLTAEGVPDIRAHIHPDHTASARVARASGLEPTDEWIGGEVIWRIA
                     APPAGA"
     gene            complement(1505..>2050)
                     /locus_tag="VFP02_09115"
     CDS             complement(1505..>2050)
                     /locus_tag="VFP02_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9543229.1"
                     /translation="GDALGPHEFETFCLLLTVAGNETTRNATSHGMHALLEHPDQMQL
                     LRDNPELLDPAIEEILRWATPVINFRRTATCATEVGGQAIAEGDALVMYYMSANRDET
                     VFEDPFTFDITRDPNDHIAFGGGGAHHCLGANLARLELQIIFKELLASTRSITQAGDV
                     ARLRSNFINGIKHMPVEVELA"
BASE COUNT          308 a          708 c          724 g          310 t
ORIGIN      
        1 cccaggagcg catggccacc gcctccaaga tgggccgcat cgcctcaccc gacgagatgg
       61 tcggaccggt cctgttcctg gtcagcgacg ccagcagcta catgaccggc caggtcctcg
      121 tcatcgacgg cggcctggcc gcctactgag ccggtaggga ctttcggccc cccagatccc
      181 gacctctggc accagacgcc ggccgaaccc gaacgtacgg tcgcagtagg tggcctcaac
      241 cgaggaccac aggggtttgg agacactcat gagcacactc acgaaggaac cgacggtcgt
      301 actcaacggc gattccggcg atacccgcgt caccggcgct gacgcgccga tcaagatcac
      361 ccgtggtcgg atcgaccacc tgctgatctg gtccggcggc gccatcgccg tcgtgctggt
      421 cgttgccggc gtcctcctga cgtgggggag cagcttctcc gaggactacg tccacgacga
      481 gctggccgcc caggccatca ccttcccgcc cgccgcggcg ctggaggaag agggtcgcga
      541 cgacctcgtg acgtacgccg accagcaggt cgacaccggg gcagaggccg aggcgtacgc
      601 cagcttcatc gccggtcacg tcgagggcat cgccgacggc gccacctacg ccgagctcgg
      661 tgacgtcgag cgtgaggccg aggccgctac ggtcgccgcc ctcgagtcag gcgcaccggc
      721 cgccgaggtc accgccctcg aggaagaggc cgccgggatc agcggacagc gggacagcat
      781 cttccggggc gagatgctcc ggggggcgct gctgagcacg ttcgcctggt cgaccatcgg
      841 tcagatcgcc ggctacgcgg ccatcgccgc cttcatcggg gctctggtca tggccggtct
      901 ggtcatcgcc ggtgtgctcc acctgagcaa gacggcgcac gcctgatcca ctagcctttc
      961 ggcccctggt acaccgcctc ggcggcagac caggggccgg ttcgcgttca tgcgccggca
     1021 ggcggagcgg cgatgcgcca gatgacctcg ccaccgatcc actcgtcggt cggttcgagc
     1081 ccggaggcgc gggcgactcg agcggatgcg gtgtggtcgg ggtggatgtg cgcccggatg
     1141 tcgggcacac cctcggcggt cagccaatcg accacggccc cggcggcctc ggaggcgtag
     1201 ccgtgcccct gccagggcac gccgatctcc cacgccacct cggccacggc ccaggagccc
     1261 ttcaccgtgg cctgcacgac accgatcgtc accccgtcgt ggcggacgac ccagttcagc
     1321 cagtccgtcc cctcgggcgt cgcgtcggtc tggctgcgga gccggtcccg aagttggtcg
     1381 agggtcggca ccgagccgcc cgtgaactcg aacatcgccg gatcggcgta ggccgccagc
     1441 agggcctcgg cgtccgagac cgtgagtggc gtgagccgca gccggcctgt cgtcaggacc
     1501 tcgctcaggc gagctcgacc tcgacgggca tgtgcttgat gccgttgatg aagttggagc
     1561 ggaggcgggc gacgtcgccg gcctgggtga tggaccgggt cgaggccagc agctccttga
     1621 agatgatctg cagctcgagc cgggccaggt tggcgccgag gcagtggtgg gccccgccgc
     1681 caccgaaggc gatgtggtcg ttgggatcgc gggtgatgtc gaaggtgaac gggtcctcga
     1741 acaccgtctc gtcccggttg gccgacatgt agtacatcac cagcgcgtcg ccctcggcga
     1801 tggcctgccc accgacctcg gtggcgcagg tggccgtgcg ccggaagttg attaccggcg
     1861 tggcccagcg caggatctcc tcgatggcgg ggtccagcag ctcgggattg tcccgcagga
     1921 gctgcatctg gtccgggtgc tcgagcaggg cgtgcatgcc gtgcgacgtg gcgttgcggg
     1981 tcgtctcgtt gccggcgacg gtgagtagca ggcagaacgt ctcgaactcg tggggcccga
     2041 gggcgtcgcc
//
LOCUS       DASTFV010000500         5192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1840805, whole genome shotgun sequence.
ACCESSION   DASTFV010000500 DASTFV010000000
VERSION     DASTFV010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840805"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VFP02_09120"
     CDS             <1..509
                     /locus_tag="VFP02_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="activates fatty acids by binding to coenzyme A; in
                     Mycobacterium may be involved in virulence; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HET9543230.1"
                     /translation="ALRPVPPGSETVGRVARTGHIPLRYHGDPEKTAATFIEHDGRRW
                     VVTGDMATVAEDGTINLLGRGSGCINTGGEKVFPEEVESVLRARSDVYDVLVVGAPDD
                     RWGERVSAIVQPADGSEPTADELIDHCRAHLAGYKVPKTVTFVDHVVRSPAGKPDYRW
                     AKAVVATD"
     gene            complement(533..2599)
                     /locus_tag="VFP02_09125"
     CDS             complement(533..2599)
                     /locus_tag="VFP02_09125"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9543231.1"
                     /translation="MEPESPSSRRLRPFLELLGLSGLAVAQPLLAVFGDAPDYFVFQG
                     SSSRDIVVFAVVIVLAPPLVLWALALLIGWGSDRRRRIAQSGAVGLLVGLLVLQIAVS
                     ADAALALALAVAAGTGAALAHRHWTDVGLWATWLAPAPLVFLALFLFSSPVSGLVQGG
                     DVDPADLGVFGSGDPPPVVMLVFDEWPLASIVRSDGTIDADLYPNVAALAGDSTWYRD
                     TTTVANLTNFAVPALLTGNLPEEGDTADTATHPENLFTLLGGNYDLDVTERITRLCPT
                     SLCATAGGRSGRGPVADVTDLLAEAREVFVDRLTPGGESGLETDGFVEPTEAVERARE
                     GAEDQRFADLLDPRPVSVNRFLRGIRAGEDPTVHFLHLLEPHTPYLDLPDGRRYGADP
                     ALRMISPREDGEPGGDRRAAAEPPARLDRQRLQLEVARVDRLLGDVLHRLRRTGLYKD
                     ALIVVTSDHGIAFQPGGPVRGLGIEPIDEAAQPELLWIPLFIKAPGQADGEVSDLPAQ
                     TIDVLPTMADRLGIDLPWTVDGEALTPEASPDPERDFFHVAGSSFATFHLDAPVPVAA
                     DLDHVLALGVDSVLVGSGPDRWWAAGPEPQLVGRPAVGPQLEAQIDGLGPFLDVDLSD
                     PVVPAVVAGRVDDDVGRVAVAVNGTIAAVVDTYDDGNGPGWFAAIVDPALLVDGFNGL
                     TIHRAG"
     gene            complement(2680..2940)
                     /locus_tag="VFP02_09130"
     CDS             complement(2680..2940)
                     /locus_tag="VFP02_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET9543232.1"
                     /translation="MRVRIPTPLRSYTGEQSAVTAEGTTVAELLADLDRQFPGLRFRV
                     VDEQHQLRPHMKVFVDQDSVRDLDTPVTDAGEVTLMQALSGG"
     gene            complement(2940..4064)
                     /locus_tag="VFP02_09135"
     CDS             complement(2940..4064)
                     /locus_tag="VFP02_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543233.1"
                     /translation="MTDTTSLFVGTRKGAWILTAAADRRNWAVDGPHFLGHIVQHAVL
                     DPRDGRTLLLAMRTGHLGPTVMRSTDAGATWAEAARPPAFTSGERLARSLRAVFWLTP
                     GHADQFGVWYAGGSPQGLFRTEDGGDTWAPVDGWNDHPMWETWSEYPEENTPDGSMLH
                     SVIVDPRDPDHLYLGLSAGGIFESTDGGNDWSTLNQDVDAVFLPEPDADYGHDPHCLR
                     MHPADPDRLYHQNHCGIYRLDRPGTKWERIGDNMPTEVGDIGFPVELHPRDPDTAWVF
                     PMDGTDVWPRTSPDGRPAAFITRDAGAHWTRQDNGLPETAWFTVKRQAMTTDEADPVG
                     VYFGTTSGEVWASADEGDSWDRILEHLPEIYSVETGRPAA"
     gene            4141..4680
                     /locus_tag="VFP02_09140"
     CDS             4141..4680
                     /locus_tag="VFP02_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543234.1"
                     /translation="MTRPENPRIDGLSARFTHLEAEDPPWIAVRSQTNRDGSESHVWE
                     RWIAFSTEPLYLALFARWDPGMIVRRHGHHSPHTLLVLEGNVSCDGAECGPGTHIELP
                     HGASFGPFVAGPDGLVAYEVMMGDPRSWSDEPEAMAAVLAEHGVTPLPDPPIELPAGL
                     EDLRAVFSAGAGPATDATD"
     gene            4686..>5192
                     /locus_tag="VFP02_09145"
     CDS             4686..>5192
                     /locus_tag="VFP02_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET9543235.1"
                     /translation="MGVTPDEADLPLIISVDDHVMEPKDLWQRELPESMRERGPKVVT
                     EQIKLEFTGGHYGFSRNDPDGTWCDLWIFDDLVVPTGLLHAPAGMPRDEQRNVPATYD
                     DFRKGTWDQTERLADMTLNHVEAAINFPNVFPRFAGQGFLEREDKDLALACLRIYNDW
                     MIDEWSGGA"
BASE COUNT          740 a         1804 c         1948 g          700 t
ORIGIN      
        1 aggcgctccg ccctgttccg cccggctccg agaccgttgg ccgggtggcc cgcaccggcc
       61 acataccgtt gcgctaccac ggggaccccg agaagacggc agccacgttc atcgagcacg
      121 acgggcgccg ctgggtcgtc accggggaca tggccacggt ggccgaggac gggacgatca
      181 acctgctggg ccggggctcg ggctgcatca acacgggcgg cgagaaggtc ttccccgagg
      241 aggtggagtc cgtcctgcgg gcccggtccg acgtctacga cgtgctcgtg gtcggcgcgc
      301 ccgacgaccg ctggggcgag cgggtgtcgg ccatcgtcca gcccgccgac ggcagcgagc
      361 cgaccgccga cgagctcatc gaccactgcc gggcccacct ggcgggctac aaggtgccga
      421 agacggtcac cttcgtggac cacgtcgtcc gctcgccggc gggcaagccc gactaccgct
      481 gggccaaggc cgtggtcgcc accgactgag ggtgctcagc cggggtgtgc ggtcacccgg
      541 cgcggtggat cgtcaggccg ttgaagccgt cgaccaggag cgccgggtcg acgatggcgg
      601 cgaaccagcc gggaccgttg ccgtcgtcgt aggtgtcgac caccgcggcg atcgtcccgt
      661 tcaccgccac ggccacccgg cccacgtcgt cgtccacccg gccggccacg acggcgggca
      721 ccaccggatc gctcaggtcg acatcgagga agggtccgag tccgtcgatc tgggcctcca
      781 gttggggccc gacggcgggc cgaccgacca gctgcggctc gggcccggcc gcccaccagc
      841 ggtccggccc gctgccgacc agcaccgaat ccacaccgag ggccaacacg tgatcgaggt
      901 cggcggcgac gggaaccggt gcgtcgaggt ggaaggtggc gaacgacgac cccgccacgt
      961 ggaagaagtc ccgctccggg tcaggtgagg cctccggggt gagggcctcg ccgtcgaccg
     1021 tccacggcag gtcgatgccg agacgatcgg ccatggtcgg cagcacgtcg atggtctggg
     1081 ccgggaggtc gctcacctcg ccgtcggcct gtcccggggc tttgatgaac agggggatcc
     1141 agagcagctc gggttgcgcg gcctcgtcga tgggctcgat cccgagcccc cgcaccggcc
     1201 cgccgggctg gaaggcgatg ccgtggtcgg aggtgacgac gatgagggcg tccttgtaga
     1261 gcccggtcct gcggagtcgg tggaggacat cccccagcag gcggtccaca cgggccacct
     1321 cgagctggag gcgctgacgg tcgagccggg ccggcggctc ggccgcggcc cgccggtcgc
     1381 cgccgggctc cccgtcctcc cgcggcgaga tcattcggag ggccgggtcg gctccgtagc
     1441 gacggccgtc gggcagatcc aggtagggcg tgtgcggctc cagcaggtgc aggaagtgga
     1501 ccgtcgggtc ctcgccggcc cggatgcccc gcaagaagcg gttgacgctc acgggtcggg
     1561 ggtccaagag gtccgcgaag cgctggtcct cggccccctc cctggcccgc tccacggcct
     1621 ccgtgggctc gacgaacccg tcggtctcca gcccgctctc gccgccgggc gtcagccgat
     1681 ccacgaagac ctcccgggcc tcggccagga ggtcggtcac gtccgccacc ggtccccggc
     1741 ccgacctacc gccggcggtg gcacacagcg aggtcggaca gaggcgggtg atcctctcgg
     1801 tgacgtcgag gtcgtagttc ccgccgagga gggtgaacag gttctcggga tgggtggcgg
     1861 tgtcggccgt gtcgccctcc tcggggaggt tgcccgtgag cagggcgggg acggcgaagt
     1921 tggtcaggtt ggccaccgtc gtcgtgtccc ggtaccaggt cgagtcgccg gccaacgcgg
     1981 ccacgttcgg gtagaggtcg gcgtcgatgg tcccgtcgct ccggacgatc gacgccagcg
     2041 gccactcgtc gaagacgagc atcaccaccg gcggcgggtc cccggagccg aagacgccga
     2101 ggtcggccgg gtcgacgtca ccgccctgca ccaggccgct caccggtgag ctgaagagga
     2161 acagggccag gaagaccagg ggggcagggg cgagccaggt cgcccacaga ccgacgtccg
     2221 tccagtggcg gtgggccaac gcggcaccag tcccggcggc gaccgccaga gccagagcca
     2281 gcgcggcatc ggcgctgacg gcgatctgga ggacgaggag tccgacgagc agcccgacgg
     2341 cgccgctctg ggcgattcgg cgcctccggt cgcttcccca cccgatgagc agggccagcg
     2401 cccagaggac gagcggcggg gcgagcacga tgaccacggc gaagacgacg atgtcccggc
     2461 tcgacgagcc ctggaacacg aagtagtcgg gggcgtcgcc gaacacggcg agcaggggct
     2521 gggcgacggc cagcccactg agcccgagga gctccaggaa gggccggagg cggcgactcg
     2581 atggtgactc gggctccacg gcccggcgga ccttaccggc cgcctgatcg ccgacggcct
     2641 cggtcgggcc tggtcatcga cctcgagctc ggtgccgggt cagccgcccg acaacgcctg
     2701 catcagggtg acctcgccgg cgtcggtgac cggggtgtcg aggtcccgga ccgagtcctg
     2761 gtcgacgaac accttcatgt gcgggcggag ctggtgctgc tcgtccacca cccggaagcg
     2821 gagtccgggg aactgacggt cgaggtcggc caggagctcg gccaccgtcg tgccctcggc
     2881 ggtgacggcc gactgctcgc cggtgtagga ccgcagcggg gtggggatgc ggacccgcat
     2941 cacgccgcgg gccggccggt ctccacgctg tagatctcgg gcaggtgctc caggatgcgg
     3001 tcccacgagt cgccctcgtc ggcgctggcc cacacctcgc cgctggtcgt gccgaagtag
     3061 accccgacgg gatcggcctc gtcggtggtc atggcctggc gcttgaccgt gaaccacgcc
     3121 gtctccggca ggccgttgtc ctggcgggtc cagtgggcgc cggcgtcgcg ggtgatgaac
     3181 gccgccggcc ggccgtcggg gctggtccgg ggccagacgt cggtgccgtc catcgggaag
     3241 acccaggccg tgtccgggtc gcgggggtgg agctcgacgg ggaacccgat gtcaccgacc
     3301 tcggtgggca tgttgtcgcc gatgcgctcc cacttcgtcc cgggtcggtc gaggcggtag
     3361 atgccgcagt ggttctggtg gtagagccgg tcgggatcgg cggggtgcat gcgcaggcag
     3421 tgcggatcgt ggccgtagtc ggcgtcgggt tcgggcagga agacggcgtc gacgtcctgg
     3481 ttcagggtgg accagtcgtt gcccccgtcc gtgctctcga agatcccgcc cgcggacagc
     3541 ccgaggtaga ggtggtccgg gtcgcggggg tcgacgatca ccgagtggag catcgagccg
     3601 tccggggtgt tctcctcggg gtactccgac caggtctccc acatcgggtg gtcgttccag
     3661 ccgtcgaccg gggcccacgt gtcgcccccg tcctcggtgc ggaacaggcc ctggggcgag
     3721 ccgccggcgt accagacgcc gaactggtcg gcgtggcccg gcgtcagcca gaagaccgcc
     3781 cgcaggctgc gggccaggcg ctcgcccgag gtgaacgccg ggggccgggc cgcctcggcc
     3841 catgtggcgc cggcgtcggt cgagcgcatg accgtcgggc cgaggtggcc cgtgcgcatg
     3901 gccaggagca gggtgcgccc gtcgcggggg tcgagcacgg cgtgctggac gatgtggccc
     3961 aggaagtgcg gcccgtcgac cgcccagttc cggcggtcgg cggcggcggt caggatccag
     4021 gcccccttcc gggtgccgac gaacaggctg gtcgtgtcgg tcatggccgc gacggtagtc
     4081 atggcgattg gaccccgccg ggctgtcgga gtcatcggtc cgtggcacga tcggaggcgc
     4141 atgacccgac ccgagaaccc ccgcatcgac gggctgagcg cccggttcac ccacttggag
     4201 gcggaggacc cgccgtggat cgcggtgcgg agccagacca accgtgacgg ctccgagtcc
     4261 cacgtctggg agcgctggat cgcgttttcg accgagccgc tctacctggc cctgttcgcc
     4321 cggtgggacc cggggatgat cgtgcgccgc cacggtcacc acagcccgca cacgctgctc
     4381 gtgttggagg gcaacgtgtc ctgtgacggg gccgagtgcg ggccggggac ccacatcgag
     4441 ctgccccacg gcgccagctt cgggcccttc gtcgccggcc cggacgggct ggtggcctac
     4501 gaggtgatga tgggcgatcc ccgttcgtgg agcgacgagc ccgaggccat ggccgctgtg
     4561 ctggccgagc acggcgtgac cccgttgccc gacccgccca tcgagctgcc cgccggcctc
     4621 gaggacctgc gggccgtgtt cagcgcgggg gccggtccgg ccaccgacgc gacagactga
     4681 cggccatggg cgtcaccccg gacgaagccg acctcccgct gatcatctcc gtcgacgacc
     4741 acgtcatgga gcccaaggac ctgtggcagc gggagctgcc cgagtcgatg cgggagcgag
     4801 ggcccaaggt ggtcaccgag cagatcaagc tggagttcac cggcggccac tacggcttca
     4861 gtcgcaacga ccccgacggc acctggtgtg acctgtggat cttcgacgac ctggtcgtgc
     4921 ccaccggcct gctccacgcc ccggcgggga tgcctcggga cgagcaacgg aacgtgcccg
     4981 ccacctacga cgacttccgc aagggcacgt gggaccagac cgagcgcctg gccgacatga
     5041 ccctcaacca cgtggaggcg gccatcaact tcccgaacgt cttcccgcgc ttcgccgggc
     5101 agggcttcct ggagcgggag gacaaggacc tcgccctcgc ctgcctgcgc atctacaacg
     5161 actggatgat cgacgagtgg tcggggggcg cc
//
LOCUS       DASTFV010000501         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1842918, whole genome shotgun sequence.
ACCESSION   DASTFV010000501 DASTFV010000000
VERSION     DASTFV010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842918"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VFP02_09150"
     CDS             complement(<1..648)
                     /locus_tag="VFP02_09150"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET9543236.1"
                     /translation="MPRTRRRRRSLGPRLAVTLNVALVFGLLTAVGGTGFLYLKLDKI
                     PRLELGDTLDEAPGGNSAQNYLLVGTDSADRLDPNDPVTNGREDLGVLSDTIMLLRVD
                     PDEPQAQLLSFPRDLFVSFPGGGEGKINSALAVGGQEALIGTIQETFGVPVHHYIEVD
                     FLGFKELVRAIDGVPFFFTTPVRDTATGLFVGHGGCNTLGPDQALAYARSRHLETL"
     gene            complement(671..2275)
                     /locus_tag="VFP02_09155"
     CDS             complement(671..2275)
                     /locus_tag="VFP02_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HET9543237.1"
                     /translation="MPGWNFADAFEVVAEALPDATAQVHGDRRYSWREFDRRSDGIAL
                     ALLEAGAEPQDKVALYVHNGPEYMEACTGAWKAGLVPVNTNYRYTADELAYLWDNADA
                     VAVVFHGTFAETADAVRDRVPVRTWLWVDDGTGPCPAWATPYEEAAGLASERTVARWG
                     RTGDDLLMLYTGGTTGMPKGVMWRQHDLFLAYAHAVAPQYDDEQDLDLMRQSLTKPGP
                     VHVPACPLMHGTGWFTALMAMMTGGSVVTLTGASFDAVELFDTVEREGVNMMAIVGDA
                     FAKPMVRALDAAPDRWDLSSLVAVISSGVMWSEASKEALLAHHPGMLLQDAFSSSEAL
                     NMGGSISSAGSAATTAKFTLGPNAVVVGDDGTPVQPGSGEIGRVAVGGFVPVGYYKDP
                     EKSAATFIEIDGKRYSMPGDYATVEADGSLSLLGRGSVCINTGGEKVFPEEVEEALKS
                     HPAIVDAVAVGVPDEKFGEAVTAVVEPDLDALIDEAEVIAHVKETLAAYKAPKRVLTI
                     DTIGRGPNGKVDYKRLRAYAEVQVNG"
BASE COUNT          358 a          815 c          904 g          370 t
ORIGIN      
        1 gagggtctcg aggtgtcggg accgggcgta ggccagggct tggtccggcc ccagcgtgtt
       61 gcagcccccg tggccgacga acaggccggt ggcggtgtcc cggaccggcg tggtgaagaa
      121 gaagggcacg ccgtcgatgg cccgcaccag ctccttgaag ccgaggaagt cgacctcgat
      181 gtagtggtgg accgggacac cgaaggtctc ctggatcgtg ccgatcaagg cttcctggcc
      241 gcccacggcc agagccgagt tgatcttgcc ctcgcccccg ccgggaaagg agacgaacag
      301 gtcgcgagga aagctcagca gctgggcctg gggctcgtca gggtcgaccc gcagcagcat
      361 gatcgtgtcc gacaggacac cgaggtcctc gcgcccgttg gtgacggggt cgttcgggtc
      421 gagacgatcg gcgctgtcgg tgccgaccag caggtagttc tgggccgaat tgccgcccgg
      481 cgcctcgtcc agggtgtcgc cgagctcgag ccgggggatc ttgtcgagct tcaggtacag
      541 gaagcccgtg cccccgacgg ccgtgagcag gccgaagacg agggccacgt tgagggtgac
      601 ggccagacgg gggcccaggg aacgacgacg tctccgggtc ctgggcaccc gaagacggta
      661 acgaggcggg tcagccgttc acctgcacct cggcgtaggc ccgcagtcgt ttgtagtcga
      721 ccttgccgtt ggggccgcgg ccgatggtgt cgatggtcag gacccgcttg ggcgccttgt
      781 aggcggcgag ggtctccttc acgtgggcga tgacctcggc ttcgtcgatc agggcgtcca
      841 ggtcgggttc gacgacggcc gtcaccgcct ccccgaactt ctcgtcgggg acgcccacgg
      901 cgacggcgtc gacgatcgcc ggatgggact tcagcgcctc ctcgacctct tcggggaaga
      961 ccttctcacc ccccgtgttg atgcagaccg agccccggcc gaggaggctg agcgagccgt
     1021 ccgcctcgac cgtggcgtag tcacccggca tcgagtaccg cttgccgtcg atctcgatga
     1081 aggtggcggc cgacttctcg gggtccttgt agtacccgac ggggacgaac ccgccgacgg
     1141 ccacccgccc gatctcgccc gagccgggct ggacgggcgt gccgtcgtcg ccgacgacga
     1201 cggcgttggg gccgagggtg aacttggccg tggtcgccgc cgagcccgcg ctggagatgg
     1261 atccgcccat gttcagcgcc tccgacgacg agaacgcgtc ctgcaggagc atcccggggt
     1321 ggtgggcgag cagtgcctcc ttgctggcct cgctccacat gacgcccgac gagatgacgg
     1381 ccacgaggct cgacaggtcc caccggtcgg gcgcggcatc caacgcccgg accatcggct
     1441 tggcgaaggc gtcgccgacg atcgccatca tgttgacccc ctcgcgctcg acggtgtcga
     1501 agagctccac ggcgtcgaac gacgcgccgg tgagcgtgac gacgctgccc cccgtcatca
     1561 tcgccatcag cgcggtgaac cagccggtgc cgtgcatgag tgggcaggcg ggtacgtgga
     1621 cgggccccgg cttcgtgagc gactggcgca tgaggtccag atcctgctcg tcgtcgtact
     1681 gcggggcgac ggcgtgggcg taggcgagga agaggtcgtg ctggcgccac atgacgccct
     1741 tgggcatgcc ggtggtgccg ccggtgtaga gcatgaggag gtcgtcgccg gtccggcccc
     1801 agcgggccac ggtgcgctcc gaagccaggc cggccgcctc ctcgtacggc gtggcccacg
     1861 ccgggcaggg cccggtgccg tcgtccaccc agagccaggt ccggacgggc acgcggtcgc
     1921 gcacggcatc cgccgtctcg gcgaaggtgc cgtggaagac gacggcgacc gcgtcggcgt
     1981 tgtcccagag gtaggcgagc tcgtcggccg tgtagcggta gttggtgttg accgggacca
     2041 gacccgcctt ccacgcgccg gtgcaggcct ccatgtactc cgggccgttg tggacgtaga
     2101 gggcgacctt gtcctgcggc tcggcgccgg cctcgagcag ggccaaagcg atgccgtcag
     2161 agcgccggtc gaactcccgc cacgagtagc ggcggtcgcc gtggacctgg gcggtggcgt
     2221 cgggcagcgc ctcggcgacc acctcgaacg catcggcgaa gttccagccc ggcatgggtc
     2281 cccctgggtc gacggcccgc gcagcatacg agggcgtccc cgacgggctc agtcgttggg
     2341 ttgccccgag gtgacgggga cgtcggcgac gacccggacc tcgtcgatga tctcgacgtg
     2401 gtcgcctgac ggctcccggg tgtgctcgag gacgagctcg cggtaga
//
LOCUS       DASTFV010000502         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1853624, whole genome shotgun sequence.
ACCESSION   DASTFV010000502 DASTFV010000000
VERSION     DASTFV010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853624"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VFP02_09160"
     CDS             complement(<1..604)
                     /locus_tag="VFP02_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HET9543238.1"
                     /translation="MRGLISVAGYVPHHRLQRGDIRGVLGKGGGKGTRSVASYDEDTT
                     TMAVEAGRLALRGAPGAAPDRLWFATSAPAYLDKTNATAVHAALRLDSGALAMDAGGA
                     IRSGVGALRSALDSSGTTLVVSADLRSGRPSSAEEASGGDGASAALVGDGDVIAEYLG
                     GASVTDEFTERWRAPGDRHTRTWEDRFGESVYPDLGRQAWE"
     gene            complement(655..813)
                     /locus_tag="VFP02_09165"
     CDS             complement(655..813)
                     /locus_tag="VFP02_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543239.1"
                     /translation="MFGRVRLRTCTAAACGRGRHRVNGPFRFAYNDLFTFADGRIRRV
                     DSYVVSLL"
     gene            812..2056
                     /locus_tag="VFP02_09170"
     CDS             812..2056
                     /locus_tag="VFP02_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HET9543240.1"
                     /translation="MRLGDVVPGEGPDNGKPLDGIRVLAAEQMQALPYATQLLGRLGA
                     HVVKVEHPVHGESGRGATPAMEDPKGRTVGATFLRNNLSKKSVGLDLKAPAGKQLFLD
                     LVPQFDVVAENFKAGTMDRLGLGYEDVRAVHPGVIYVSVSGFGNRDDSPYREWPAYAS
                     IVEAMSGIYAWAQRPGEPPRPNPVGALGDISSALFGVIGILAALRHRDRTGVGQYVDI
                     AMLDAVVAMTDVVSNLWSLGQDPDAPLPMILDAFATKDGYLNMQVVREHQFAALVTLI
                     GRPEWVDDPRFAERLGWAEYLESDIRPAIEAWLADKTRLEATHLLSDVGIAAGPVHTS
                     PEIIADEHVAKRHMIVEMERTDGVADPVLIPGNPVKLSAGAEGPETRIPWVGEHTTEV
                     LQAELGLSGVELDQLRADGVIT"
     gene            2074..2424
                     /locus_tag="VFP02_09175"
     CDS             2074..2424
                     /locus_tag="VFP02_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543241.1"
                     /translation="MAGLLLLTACSGDDVPVSTSTTRAATPSSVAPPTSEPPACADLA
                     DDYLDTFFALGAGTPRNPEATRVRLPVAALLAIDQQARDAGCRDFVDVACSAYAELEA
                     QGLRATNSNPPRSC"
BASE COUNT          369 a          938 c          877 g          325 t
ORIGIN      
        1 cctcccaggc ctgacggccg aggtcggggt agacggactc gccgaagcgg tcctcccacg
       61 tgcgggtgtg acggtcgcct ggggcccgcc agcgctcggt gaactcgtcg gtgaccgagg
      121 cgccgccgag atactcggcg atcacgtcgc cgtcgccgac gagggcggcg gaggcgccgt
      181 caccaccgga ggcctcctcg gcgctggacg ggcggccgga acgcaggtcg gcgctgacga
      241 ccagcgtggt cccactcgag tcgagggccg agcgcagcgc accgacgccg gagcggatgg
      301 cgccgccggc gtccatggcc agcgcgcccg agtccagccg aagtgcggcg tggacggccg
      361 tggcattggt cttgtccagg taggcggggg ccgacgtggc gaaccacaga cggtccggcg
      421 cggcgccggg agcgcccctg agggccagcc gaccggcctc gacggccatg gtcgtcgtgt
      481 cctcgtcgta ggaggccacg gagcgggtgc ccttgccgcc gcccttgccc agcacgcccc
      541 ggatgtcgcc ccgttggagg cggtggtgag gcacgtagcc cgccacactg atcagtcctc
      601 gcacggcttc ggagggtact tctggacgaa cccggcccgg gcattgggcg cgggtcagag
      661 caacgacacc acgtaggagt cgacccgccg gatgcggccg tcggcgaagg tgaacaggtc
      721 gttgtaggcg aagcggaacg gcccgttgac ccggtgccga ccccgaccac atgcggcggc
      781 ggtgcaggtt cggagtcgta ccctcccgaa catgcgactg ggtgacgtgg ttcccgggga
      841 ggggccggac aacggcaagc ctctggacgg gatccgcgtg ctggcggccg aacagatgca
      901 ggccctcccg tacgccacgc agctcctggg gcgcctcggt gcccacgtcg tcaaggtcga
      961 gcacccggtc cacggcgagt ccgggcgggg ggcgacgccg gccatggagg acccgaaggg
     1021 ccggaccgtc ggcgccacct tcctgcgcaa caacctgtcc aagaagagcg tgggcctcga
     1081 cctgaaggca ccggccggca agcagctctt cctcgacctc gtcccccagt tcgacgtcgt
     1141 ggccgagaac ttcaaggcgg ggacgatgga ccgcctcggt ctcggctacg aggacgtccg
     1201 agccgtgcac cccggcgtga tctacgtgtc ggtctccggc ttcgggaacc gggacgacag
     1261 cccctaccgg gaatggcccg cgtacgcctc gatcgtcgag gccatgtccg gcatctacgc
     1321 ctgggcccag cgcccgggcg agccgccccg gcccaacccg gtcggcgccc tcggcgacat
     1381 cagcagcgcc ctgttcggcg tcatcgggat cctggccgcc ctccgccacc gggatcgcac
     1441 gggcgtcggc cagtacgtcg acatcgccat gctcgacgcc gtcgtggcca tgaccgacgt
     1501 cgtgtccaac ctctggtccc tgggccagga cccggatgcc ccgctgccga tgatcctcga
     1561 cgccttcgcc accaaggacg ggtacctcaa catgcaggtc gtccgggagc accagttcgc
     1621 cgccctggtg accctgatcg gccgcccgga gtgggtcgac gacccccgct tcgccgagcg
     1681 cctcggctgg gccgagtacc tggagagcga catccggccc gccatcgagg cctggctggc
     1741 cgacaagacc cggctggagg ccacccacct gctcagcgac gtgggcatcg ccgccggacc
     1801 cgtccacacg tcgcctgaga tcatcgccga cgagcacgtg gccaagcgcc acatgatcgt
     1861 ggagatggag cgcaccgacg gcgtggccga ccccgtcctc atcccgggta acccggtgaa
     1921 gctgtcggct ggggccgagg gccccgagac ccgcatcccg tgggtcggcg agcacacgac
     1981 cgaggtcctg caggccgagc tcggcctgtc cggcgtcgag ctggatcaac tgcgggccga
     2041 cggggtcatc acctgaaggg cgtccccgtc ctggtggccg ggctcctgct gctcacggcg
     2101 tgcagcggcg acgacgtccc ggtgtccacc tcgacaaccc gcgcggccac gccgtcgagc
     2161 gtggccccgc ccacctccga gccgccggcc tgcgccgacc tggccgacga ctacctcgac
     2221 accttcttcg ccctcggcgc gggcacgccc cggaaccccg aggccaccag ggtccgcctg
     2281 cccgtggccg ccctgttggc catcgaccag caggcccgcg acgccggctg ccgtgacttc
     2341 gtcgacgtcg cctgcagtgc ttacgccgag ctcgaggccc aaggcctccg ggccaccaac
     2401 agcaacccac cccgcagctg ctgacaccca cagcggacgg tcagtcgacg agctcgtcgc
     2461 cgaggccgac gcggtcgacc tcgcaggccg gggtgaggcc ggcgacgtc
//
LOCUS       DASTFV010000503         6344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1854210, whole genome shotgun sequence.
ACCESSION   DASTFV010000503 DASTFV010000000
VERSION     DASTFV010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854210"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /locus_tag="VFP02_09180"
     CDS             complement(<1..1591)
                     /locus_tag="VFP02_09180"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET9543242.1"
                     /translation="MPIRPRDHSMGVLVNSVSADKIRNVALVGHGGVGKTSLAEALLH
                     RAGVTTRQGRVEDGNTVCDYDATEHERGISLSLALAPFEWNGFKINLIDTPGYADFVG
                     DVWAALQVADLAVFVVSAVEGVEVQHEEVWRRAAELGLPRLVFINKLDRERASFQRTL
                     DQLRDTFGAGIAPLELPIGEEAAFVGIADLLTDTAWTYTEGKGTKGEIPEEMAELEHQ
                     VHDNLVEGIVVADDEQLERYLEGDIPSMEELEHTLAHGVASASVFPVVCGSATASIAV
                     DRLADFIVEIGPAPGDRPAVEVEAAGEPFEVASDPAGDPLAVVFKTIADPYVGHVSLF
                     KVLSGTIRPDDHLVNTRTGHDERLHNLSALRGKETVPVSSLPAGDIGAAAKLTDTRTG
                     DTLAPKGKPVKVPAADRPRPVLSVAIAARNSSDEDKLATALHRLQEEDPAIQIHRDDQ
                     THQTLLRGMGETHLSISLDKLAKKYGVQVDTQDVKVRYLETITGEGDAEGRHKKQSGG
                     RGQFGVASLRIEPLDRGGGFVF"
     gene            1708..2403
                     /locus_tag="VFP02_09185"
     CDS             1708..2403
                     /locus_tag="VFP02_09185"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9543243.1"
                     /translation="MFDGRFRTNIEQGLKPVGANLRKTGISADHLTVLGILMATAAAV
                     TIGAGFLRAGLLLLALAGVPDLLDGAVAKASGTASTRGAFFDSVSDRLSDALLFGGVA
                     WYLQTEYGGRIALLPYAVFAAAALVSYQRAKADALGFDARGGLMERAERFILLGLGLL
                     FSELLIAVLWVMLVATSGTAIYRFVKVWRQADRPPAPTRAPTRTRRRRSGTTRSMADR
                     RVAWRERMRSRRP"
     gene            2408..3304
                     /locus_tag="VFP02_09190"
     CDS             2408..3304
                     /locus_tag="VFP02_09190"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HET9543244.1"
                     /translation="MNRTVAAYKAAASITRGLPGPLASLLGRGGALGASALLDDERLI
                     IERNLRRAYGPDYRGLPLRRSVQAVLDNYARYWVESFRLPGTSSEELDAGMSFDGFHR
                     IENAVDSGIGPILALPHLGAWEWGGFWLASTWKHEVTVVVEGLEPPELFEWFAALRRS
                     FGMTVVPLGPGAGATVVTAIKQAHVTCLLSDRDINGSGVEVEFFGERTTLPGGPATLA
                     LRTGAALLPAAVYYRGKGHHATIREAVPAERRGRLRDDVQRVTQVLAYELEALIRAEP
                     EQWHLLQPNWPSDHEALAAARA"
     gene            3317..4396
                     /locus_tag="VFP02_09195"
     CDS             3317..4396
                     /locus_tag="VFP02_09195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9543245.1"
                     /translation="MRVGLLCPYSLSIPGGVQGQVLGLARALRRRGHEARVLAPCDGP
                     PPDAFVTPLGKSVPAAANGSVAPLAPDPAAQLRTMRALWDEDFDVLHLHEPFAPGPCM
                     TALMIKPAPTIGTFHAAGESFAYKHMGAGTRFLNRRLDLRCAVSEDARALASSAVSGA
                     FTLLFNGVELAAYRDGEPWPTEGPTIFFCGRHEPRKGLAVLLEAMAGLPGDVRLWVGS
                     DGPETAALQARWAGDPRVEWLGRLSDEEKVARLRGADVFCAPSVRGESFGVVLIEAMA
                     AETPIVASELPGYANVARAGQDAELVPPGDPRALAAAITRLLEDDGLRRRVVASGRLR
                     AEEFSMDRLADRYVELYGSLVESRK"
     gene            4393..5070
                     /locus_tag="VFP02_09200"
     CDS             4393..5070
                     /locus_tag="VFP02_09200"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET9543246.1"
                     /translation="MTVLLIVLAIIAVIVIVLVVLYNGLVKLRNRIENAWAQIDVQLK
                     RRYDLIPNLVETVKGYASHERETLEAVTQARNMAVNAQGVGEQAQAENMLSGALKSLF
                     AVSEAYPDLKANENFLNLQEELTGTEGRIAYSRQFYNDTVLRYNTKIQTFPAVVIAGM
                     MRFTPREYFEAEPESRDAVQVSFNEPPSVAGTPAPAPPAAPAAPAAPSVPPTDAGGPA
                     PGTPPPA"
     gene            5143..5985
                     /locus_tag="VFP02_09205"
     CDS             5143..5985
                     /locus_tag="VFP02_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET9543247.1"
                     /translation="MVVVILVGLAFNTIIGYGPVGVVIAVVIAAGLAIGSYWKSDEIA
                     LRMSRAQPASVEEYPRLHNIVEGLCIAAGLPKPRLYVIQDDAPNAFATGRDPKHAAIA
                     ATTGLLDKMNRIELEAVLAHELSHVKNYDILVSTLAVTMVGAVALLSDIGIRSLWFGG
                     GRRSRDDNSGGGIVMILALVFLVLAPIVAKIMQLAVSRRRETLADFSAVEITRYPPGM
                     VSALEKLRDDQTVVHSGSRATAHLWIEQPMARTKEEGPFSRLNHLFDTHPPLEERIAA
                     LREL"
     gene            6004..>6344
                     /locus_tag="VFP02_09210"
     CDS             6004..>6344
                     /locus_tag="VFP02_09210"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HET9543248.1"
                     /translation="MDTKRKALIALGSAVAILGILLFVVTRGGDDEPPPPTTAVTTTV
                     PSGYWPLTGLPAEPGDPINRPAIAVKVSNSSDARPQIGLDKADIVIEERVEGITRLVA
                     VFQSTSSAPVGP"
BASE COUNT          868 a         2349 c         2146 g          981 t
ORIGIN      
        1 cgaagacgaa gccgccaccc cggtccagcg gctcgatgcg caacgaggcc acgccgaact
       61 ggccccggcc ccccgactgc ttcttgtggc ggccctccgc gtcaccctcg ccggtgatcg
      121 tctccaagta gcggaccttc acgtcctgag tgtcgacctg caccccgtac ttcttggcca
      181 gcttgtccaa gctgatgctc agatgggtct cgcccatgcc ccgtagcagg gtctggtggg
      241 tctggtcgtc gcgatggatc tggatggccg gatcctcctc ctgcagccgg tgcagggccg
      301 tggccaactt gtcctcgtca ctcgagttgc gggcggcgat ggcgactgac agcaccgggc
      361 gaggtcggtc cgccgccggg accttcaccg gcttgccctt gggggccagg gtgtcaccgg
      421 tgcgggtgtc ggtcagcttg gccgccgccc cgatgtcgcc ggcgggcagc gaggacaccg
      481 gcaccgtctc cttgccccgc agggcggaca ggttgtgcag gcgctcgtcg tggccggtcc
      541 gggtgttgac caggtggtcg tcgggtcgga tggtgcccga cagcaccttg aagagcgaga
      601 catggccgac gtaggggtcg gcgatggtct tgaagacgac ggccagcggg tcgccggcgg
      661 ggtcggaggc cacctcgaac ggctcgccgg cggcctcgac ctcgacggcg ggccggtcgc
      721 ccggcgccgg gccgatctcc acgatgaagt cggccagccg gtcgaccgcg atgctggcgg
      781 tggccgagcc gcacaccacg gggaagaccg atgccgaggc cacgccgtgg gcgagggtgt
      841 gctcgagctc ctccatcgac gggatgtcgc cctcgagata gcgctcgagc tgctcgtcgt
      901 cggccacgac gatgccctcg accaggttgt cgtggacctg gtgctcgagc tcggccatct
      961 cctcggggat ctcgcccttg gtccccttgc cctccgtgta ggtccaggcc gtgtcggtca
     1021 gcaggtccgc gatccccacg aacgccgcct cctcgccgat ggggagctcc agcggggcga
     1081 tcccggcgcc gaaggtgtcc cggagctggt ccagggtgcg ttggaacgag gcccgctccc
     1141 ggtcgagctt gttgatgaac acgagacggg gcagccccag ctcggccgcc cgccgccaga
     1201 cctcctcgtg ctggacctcg acgccctcga cggcgctgac gacgaacacg gccaggtccg
     1261 ccacctgcaa cgcggcccag acgtcgccca cgaagtcggc gtaccccggc gtgtcaatca
     1321 ggttgatctt gaagccgttc cactcgaacg gggccagggc caacgacagg gagatcccgc
     1381 gctcgtgctc ggtggcgtcg tagtcacaga ccgtgttgcc gtcctcgacc cggccctggc
     1441 gggtcgtgac cccggctcgg tgcagcaggg cctcagccaa gctcgtcttc ccgacaccgc
     1501 cgtggcccac cagggcaacg ttgcggatct tgtcagccga taccgagttc acgagcaccc
     1561 ccatgctgtg gtcccgcggt ctgatgggca cgataggcga acgttcgcgt ccgcacttcc
     1621 gcgacgtccc gttcttgctg agcgtcgaac aggtagtctg ttgggacttc tcgcctccgg
     1681 atcagctccg gaaaggactt ctccccgatg ttcgacggtc gcttccggac aaacatcgaa
     1741 cagggcctca agcccgtcgg tgccaacctc cgcaagacgg ggatctccgc cgaccacctg
     1801 acggtgctcg ggatcctcat ggccacggcc gccgccgtca ccatcggagc cggcttcctc
     1861 cgggccggtc tgttgctgct ggccctggcc ggcgtgccgg acctgctcga cggtgcagtg
     1921 gccaaggcct cgggaacggc gtccacccgt ggtgccttct tcgactcggt ctcggaccgc
     1981 ctcagcgacg ccctcctgtt cggtggcgtg gcctggtacc tgcagaccga gtacggcgga
     2041 cgcatcgccc tgctgcccta cgccgtcttc gcggccgccg ccctggtctc ctaccagcgg
     2101 gccaaggccg acgccctcgg cttcgacgct cggggcggcc tcatggagcg ggccgagcgc
     2161 ttcatcctcc tgggcttggg cctgctcttc agcgagctgc tcatcgccgt cctgtgggtg
     2221 atgctggtgg ccacgtcggg cacggcgatc taccgcttcg tgaaggtctg gcgacaggcc
     2281 gaccgcccgc ccgcgccgac ccgagcgccg acccggaccc ggcggcggcg cagcggcacg
     2341 acccgttcca tggccgaccg tcgggtcgcc tggcgcgagc gcatgcgcag ccggcggccc
     2401 tgacaccctg aaccgcacgg tcgccgccta caaggcggca gcgtccatca cccgcggcct
     2461 tcccggaccg ctggcgtccc tcctgggccg cggcggagcc ctcggcgcgt cggccctcct
     2521 cgacgacgag cgcctgatca tcgagcgcaa cctgcgccgg gcctacggcc ccgactaccg
     2581 cgggctgccc ttgcgccgct ccgtccaagc cgtcctcgac aactacgccc gctactgggt
     2641 ggagtccttc cggctgccgg gcacgtcgtc ggaggagctg gacgcgggca tgtccttcga
     2701 cggcttccat cggatcgaga acgcggtgga cagtgggatc ggccccatcc tggccctgcc
     2761 ccacctcggg gcctgggagt ggggcggctt ctggctggcc tcgacctgga agcacgaggt
     2821 gaccgtcgtg gtcgaggggc tcgagccccc ggagctcttc gagtggttcg cggccctccg
     2881 ccgttcgttc ggcatgaccg tcgtgcctct cgggccgggt gccggggcga ccgtcgtcac
     2941 ggccatcaag caggcccacg tcacctgcct gctctccgac cgcgacatca acggcagcgg
     3001 ggtcgaggtc gagttcttcg gcgagcgcac caccctgccc ggcggtcccg ccaccctggc
     3061 gctgcggacg ggggcggccc tgttgcccgc ggccgtgtac taccggggca agggccacca
     3121 cgccacgatc cgggaggcgg ttcccgccga acgtcgaggg cggctgcgcg acgacgtcca
     3181 gcgcgtcacc caggtgctgg cctacgagtt ggaggctctc atccgggccg agccggagca
     3241 gtggcacctg ctccagccca actggcccag tgaccacgaa gccctcgccg cggcccgcgc
     3301 ctagcttcct cgcatcgtgc gcgtcggcct gctgtgcccc tacagcctct cgatccccgg
     3361 tggggtccag ggccaggtcc tcgggctggc ccgcgccctc cgccgccggg gccacgaggc
     3421 ccgcgtcctg gccccctgcg acggcccccc gcccgacgcc ttcgtgaccc cgctgggcaa
     3481 gagcgtgccg gccgcggcca acggctcggt cgccccgttg gccccggacc cggcggccca
     3541 gctccgcacg atgcgggccc tgtgggacga ggacttcgac gtcctccacc tgcacgagcc
     3601 gttcgccccc ggaccgtgca tgacggccct gatgatcaag cccgcgccca ccatcggcac
     3661 gttccacgcg gccggcgaga gcttcgccta caagcacatg ggggccggga cccgcttcct
     3721 caaccgccgc ctggacctgc gctgtgccgt gtccgaggac gcccgggcgc tggcctccag
     3781 cgcggtctcg ggggccttca ccctgctctt caacggcgtg gagctggccg cctaccggga
     3841 cggtgagccg tggcccaccg aggggccgac catcttcttc tgcggtcgcc acgagccccg
     3901 taagggcctc gccgtgctgt tggaggcgat ggcgggcctc cccggagacg ttcgcctctg
     3961 ggtgggcagt gacgggccgg agaccgcggc gctccaggcc cgatgggccg gtgatccgcg
     4021 ggtcgagtgg ctgggtcggc tcagcgacga ggagaaggtg gcccgtctcc gcggggccga
     4081 cgtgttctgc gccccctcgg tccggggcga gtcgttcggc gtcgtgctga tcgaggcgat
     4141 ggccgccgag acgcccatcg tggcctccga gctgcccggg tacgccaacg tggcccgcgc
     4201 cggtcaggac gcggagctgg tcccgcccgg tgacccgcgg gcgctggccg cggccatcac
     4261 ccggctgctc gaggacgacg gattgaggcg gcgcgtggtg gcgtccggcc gcctgcgggc
     4321 ggaggagttc tcgatggacc gcctggccga ccgctacgtc gagctgtacg gctcgcttgt
     4381 agagtcgcgc aagtgaccgt gctcctcatc gtcctcgcca tcatcgcggt gatcgtgatc
     4441 gtcctcgtcg tgctctacaa cggcctggtc aagctccgga accggatcga gaacgcctgg
     4501 gcccagatcg acgtccagct caagcgccgc tatgacctga tccccaacct ggtcgagacg
     4561 gtcaagggct acgccagcca cgagcgggag accctcgagg ccgtgaccca ggcccggaac
     4621 atggccgtga acgcgcaggg ggtgggggag caggcgcagg ccgagaacat gctgagcggc
     4681 gccctgaagt cgctgttcgc ggtcagcgag gcgtacccgg acctgaaggc gaacgagaac
     4741 ttcctgaacc tgcaagagga gctgaccggc accgagggcc gcatcgccta ctcgcggcag
     4801 ttctacaacg acaccgttct ccgctacaac accaagatcc agacgttccc ggccgtcgtc
     4861 atcgccggga tgatgcgctt caccccccgc gagtacttcg aggccgagcc cgagtcccgc
     4921 gacgccgtcc aggtctcctt caacgaaccg ccgtcggtcg ccggcacgcc ggccccggcc
     4981 ccgcccgccg ccccggccgc gcccgccgcg ccgtcggtgc cccccaccga tgccggtggc
     5041 ccggccccgg gtacgccgcc tccggcctga tcgaggtcga tccacctgta cgacgaggtc
     5101 aaggccaaca agcgccgctc catcctcctg atcgccgcct tcgtggtggt ggtgatcctg
     5161 gtcgggctcg cgttcaacac gatcatcggg tacggcccgg tcggggtcgt catcgccgtg
     5221 gtcatcgcgg ccggcctggc catcggcagc tactggaagt ccgacgagat cgccctccgc
     5281 atgagccggg cccagcccgc gtcggtggag gagtaccccc ggctccacaa catcgtcgaa
     5341 ggcctgtgca tcgccgccgg cctgcccaag cctcgcctct acgtcatcca ggacgacgcc
     5401 ccgaacgcct tcgccacggg gcgggatccg aagcacgccg ccatcgccgc cacgaccggc
     5461 ctgctcgaca agatgaaccg catcgagctg gaggcggtgc tggcccacga gctcagtcac
     5521 gtcaagaact acgacatcct cgtgtccaca ctggcggtga cgatggtcgg cgccgtcgcc
     5581 ctcctctccg acatcggcat ccggtccctg tggttcggcg gtggtcgccg gagccgggac
     5641 gacaactccg gcggtgggat cgtgatgatc ttggccctgg tcttcctggt cctcgcaccg
     5701 atcgtggcca agatcatgca gttggcggtc agccgccgcc gggagacgtt ggccgacttc
     5761 tccgccgtgg agatcacccg gtacccgccc ggcatggtgt ccgccctgga gaagctgcgc
     5821 gacgaccaga ccgtggtcca ctcggggtcg cgcgccaccg cccacctctg gatcgagcag
     5881 ccgatggccc ggacgaagga agagggcccg ttctcgaggc tgaaccacct cttcgacacc
     5941 catccaccgc tcgaggagcg catcgccgcg ctgcgtgagc tctagcgccg gagcatcagc
     6001 cccgtggata cgaagcgaaa ggcgctcatc gccctcggct cggccgtcgc gatcctgggg
     6061 atcctgctgt tcgtggtgac ccggggcggt gatgacgagc cgcccccgcc gacgacggcg
     6121 gtgaccacga cggtgccgtc ggggtactgg ccgctcaccg ggctcccggc agagccgggc
     6181 gacccgatca accgtccggc gatcgccgtg aaggtctcca actcatccga cgcccgtccg
     6241 cagatcggcc tggacaaggc cgacatcgtg atcgaggagc gggtcgaggg catcacccgg
     6301 ctggtcgccg tgttccagtc gacctcctcg gcaccggtgg gccc
//
LOCUS       DASTFV010000504         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1855899, whole genome shotgun sequence.
ACCESSION   DASTFV010000504 DASTFV010000000
VERSION     DASTFV010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855899"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VFP02_09215"
     CDS             <1..782
                     /locus_tag="VFP02_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9543249.1"
                     /translation="GEAAACDPLPLKNDGTLTVATGEPAFPPWVSGVDADGNDDPTLG
                     EGFESALVYAVAEQLGVAEADVEWVRTGFDEAIAPGEKDYDFNIQQFGITPERDEVVD
                     FSDGYYDVQQVVIAAEGSPISEATSVGELADFNLGAAIGTTSLDYIDDVIQPDTEAAV
                     FDDNAAATAAFDAGQVDGIVYDLPTAYFITSVEIPDSAIVGVLPQAGADPEQLGMLFE
                     TDSPLIPCVNEALATLRDDGTLAALQEEWLSAGGDIPTLSE"
     gene            805..1701
                     /locus_tag="VFP02_09220"
     CDS             805..1701
                     /locus_tag="VFP02_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET9543250.1"
                     /translation="MVEPAADPSAVVGGVGSGPPLPPGRVASWRAEFREEGAVGALIA
                     FLSTIVVFGLIIWFVVHSETWPQVRDQFLSREDFVDSFPDVLSGFWLDLKMFVVAQAV
                     ILVFALVVAVIRNLRGPAFFPLRFLAVLYIDLFRGIPLLLVILLLGFGVPALQLDGVP
                     TDPRFWGVTALVLSYSAYTAEVYRAGIDSVHESQRSAARALGLTQWQALRFAVLPQAV
                     RNVIPALLNGLVSLQKDVALVSVLGIREAVREAQIYTSRTFNYTSYMAATVLFLLASV
                     PIARFADWYTARDRRRRSQSIA"
     gene            1698..2459
                     /locus_tag="VFP02_09225"
     CDS             1698..2459
                     /locus_tag="VFP02_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET9543251.1"
                     /translation="MTGDASTQRLEVRGLRKAFGPKVVLDGIDLSVAEHEVVAVIGPS
                     GCGKSTLLRCIDLLERIDGGSIWLSGEAVTGVGVNANDVRKRIGIVFQAFNLFPHLTV
                     LDNVTLAPRRVHGQPKRVAEDQAREVLDRLGLGDKVTDYPDRLSGGQQQRAAIARALV
                     TGPELLLLDEVTSALDPELVGEVLAVIRDLAAAGMTMVLATHEMGFARDVAHRVAFMH
                     EGRVLEEGLPATLFAEPREERTRQFLKRVTDAGRL"
     gene            2461..>2781
                     /locus_tag="VFP02_09230"
     CDS             2461..>2781
                     /locus_tag="VFP02_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9543252.1"
                     /translation="MSNGNGRLDGRVAFVTGAASGIGAACAQRFVDEGARVAGFDLQD
                     PAEGIPLAGFWSLDVRDEPAIASAVAAAVAEVGVPTVLVNAAGVSGTASVTDLAEEEW
                     DRIVD"
BASE COUNT          373 a         1044 c          939 g          425 t
ORIGIN      
        1 gaggtgaggc ggcggcctgc gacccgctgc ccctgaagaa cgacgggacc ctcacggtgg
       61 ccactggcga gccggccttc ccgccctggg tctccggcgt cgacgccgac ggcaacgacg
      121 acccgaccct cggtgagggc ttcgagtccg ccctcgtgta cgccgtagcc gagcagctcg
      181 gtgtggccga agccgacgtc gagtgggtcc gcacgggctt cgacgaggcc atcgccccgg
      241 gcgagaagga ctacgacttc aacatccagc agttcggcat caccccggag cgcgacgagg
      301 tcgtggactt ctccgacggc tactacgacg tgcagcaggt ggtcatcgcg gccgagggat
      361 cgccgatctc cgaagccacc tcggtcggcg agctggccga cttcaacctc ggtgccgcca
      421 tcgggaccac cagcctcgac tacatcgacg atgtgatcca gccggacacc gaggccgccg
      481 tcttcgacga caatgccgcg gccacggccg ccttcgacgc cggccaggtg gacggcatcg
      541 tctacgacct gcccaccgcc tacttcataa cctcggtcga gatcccggac tcggccatcg
      601 tgggcgtgct gccgcaggcc ggggccgacc ccgagcagct gggcatgctc ttcgagacgg
      661 acagccccct gatcccctgc gtcaacgagg ccctggccac gctgcgcgac gacggcacgc
      721 tggccgccct ccaggaggag tggctgagcg cgggcggcga catcccgacc ttgtcggagt
      781 gacgccccga ccctgacgga gcacgtggtg gagcccgcag ccgacccctc ggccgtcgtc
      841 ggcggggtcg gctccgggcc gccactgcct ccgggccggg tcgcctcgtg gcgagccgag
      901 ttccgggaag agggggcggt cggcgccctc atcgccttcc tcagcacgat cgtcgtcttc
      961 gggctgatca tctggttcgt cgtccacagc gagacctggc cccaggtccg ggaccagttc
     1021 ctcagccgcg aggacttcgt cgacagcttc cccgacgtgc tgtccggctt ctggctggac
     1081 ctgaagatgt tcgtcgtggc ccaggcggtg atcctggtct tcgccctggt ggtcgccgtg
     1141 atccgcaacc tccggggtcc ggccttcttc cccctccgct tcctggccgt cctctacatc
     1201 gacctgttcc gcggcatccc gctcctgctg gtgatcctgc tcctcggctt cggcgtgccc
     1261 gcgctgcagc tggacggggt gccgaccgat ccccgcttct ggggcgtcac cgccctcgtc
     1321 ctctcgtact cggcctacac cgccgaggtg taccgggccg gcatcgactc ggtccacgag
     1381 agccagcgtt ccgccgcccg ggccctcggc ctgacccagt ggcaggccct gcgcttcgcc
     1441 gtgctccccc aggccgtgcg caacgtcatc cccgccctgc tcaacggcct ggtctcgctg
     1501 cagaaggacg tggccttggt cagcgtgctc ggcatccggg aggcggtgcg ggaggcgcag
     1561 atctacacgt cccgcacgtt caactacacc tcctacatgg ccgccaccgt cctcttcctg
     1621 ctggccagcg tccccatcgc ccgcttcgcc gactggtaca cggcccggga ccggcgccgc
     1681 cgctcccagt cgatcgcgtg accggcgacg cctcgaccca acgcctcgag gtccggggcc
     1741 tgcgcaaggc cttcgggccg aaggtggtgc tggacggcat cgacctgtcg gtggccgagc
     1801 acgaggtggt ggccgtcatc gggcccagcg gctgcgggaa gtcgaccctc ctgcggtgca
     1861 tcgacctgct cgagcgcatc gacggcgggt ccatctggct gagcggcgaa gccgtgaccg
     1921 gcgtcggcgt caacgccaac gacgtgcgca agcgcatcgg catcgtcttc caggccttca
     1981 acctgttccc ccacctgacc gtgctcgaca acgtgaccct ggccccccgc cgggtccacg
     2041 gccagcccaa gcgggtcgcc gaggaccagg cccgcgaggt cctcgaccgc ctcggcctcg
     2101 gcgacaaggt caccgactac ccggatcggc tctcgggtgg ccagcagcaa cgagcggcca
     2161 tcgcccgggc cctggtcacc gggcccgagc tcctgctcct cgacgaggtg acctcggccc
     2221 tggaccccga gctggtcggc gaggtcctgg ccgtcatccg ggacctggcc gccgcgggca
     2281 tgaccatggt gctggccacc cacgagatgg gcttcgcccg cgacgtggcc caccgcgtgg
     2341 ccttcatgca cgagggccgg gtcctcgagg agggcctgcc cgccaccctg ttcgccgagc
     2401 cccgcgagga gcggacgcga cagttcctga agcgcgtcac ggacgcggga aggttgtgag
     2461 atgagcaacg gcaacggccg tctggacgga cgggtcgcct tcgtcaccgg ggcggcctcg
     2521 ggcatcgggg cggcgtgtgc gcagcgcttc gtggacgagg gggccagggt ggccggcttc
     2581 gacctccagg acccggccga agggatcccg ctggccggct tctggtccct cgatgtccgc
     2641 gacgagcccg ccatcgcctc ggcggtcgct gccgccgtcg ccgaggtcgg ggtccccacg
     2701 gtcctggtca acgccgccgg cgtgtcgggg acggcgagcg tcacggacct ggccgaggag
     2761 gagtgggacc gcatcgtcga c
//
LOCUS       DASTFV010000505         2316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1874469, whole genome shotgun sequence.
ACCESSION   DASTFV010000505 DASTFV010000000
VERSION     DASTFV010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874469"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1527)
                     /locus_tag="VFP02_09235"
     CDS             complement(187..1527)
                     /locus_tag="VFP02_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543253.1"
                     /translation="MTAGVTRARFTALAERNPLPDGTLTVGGGLVVNGVMSYVFLGIA
                     ARTLGVDAFGPLSVLWALIYLVGPGFFLPLEQEVSRSLANRWARGVGVGPLVKQAGLL
                     GTALIGALVLTTLVAAPLLLDHLFDDQVLILIGFVLAILGYSAVHLARGTLAGLGRFR
                     GYALFFVYENSLRVAAAIVLALLGVDTAGPYAVAVGLAPGVAIVLALLGEHDLASDGP
                     PAPWGELGAALGSLLAASVLTAFLLYAGVLAVELLADESESDKAGVFLAGLAVSRVPV
                     FLFQAVQAALLPKLSALAGAGRFHEFRDRLRRLLAAVGVIGVAGVAGAALLGPFLIDI
                     LFGDDFVLTRLDLGLLALSSAAFMLAVALGQALIALEGQSRVAFGWLIGVLAFLGITA
                     MGDDLFLRVEAGLAAGSAVAAVVIGGLARHRLHGKLRTHPHTLTTIDRETEIEA"
     gene            complement(1568..>2316)
                     /locus_tag="VFP02_09240"
     CDS             complement(1568..>2316)
                     /locus_tag="VFP02_09240"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HET9543254.1"
                     /translation="TIVLVTHSLGQVQSLCDQAIWMERGEVREQGHAIDIARSYLQQV
                     NITEAEAGFEGDLGDADLILEGRGERRGTGEITIEHVSFVNAHRAVTPFANSGRPLVV
                     RIHYKAHQPIKNPSFAVRFHTDSGTMITAPNSRRGRLNTGMVDGEGVIFYKIPRMHLT
                     PGTYHLTAGVYDENNLHIFDQREREFELRVQPGSANDAEGYLDLGGQWAMPTGSKDEE
                     VVEQGASMGVDRVDGVLDDRATAVGRDGAA"
BASE COUNT          376 a          795 c          864 g          281 t
ORIGIN      
        1 cggtcaccga cggcagctcc ggcaggtcgg agccggggga cggatccgcg gcgcccgcaa
       61 ccagtccggc acccgagagg gcggccgcgg cgagcacggt gccggcgatc aggtagcgag
      121 gggcgggacg catgggccaa gacccatcgg tgccgccctg accacgttga gtgctcgaag
      181 acctggtcac gcctcgatct cggtctcccg gtcgatcgtg gtcaacgtgt gggggtgggt
      241 ccgcagcttg ccgtggaggc gatggcgggc caggcccccg atgacgacgg cggcgacggc
      301 cgagccggcg gcgaggccgg cttcgacccg caggaagagg tcatcgccca tggcggtgat
      361 ccccaggaag gcgagcacgc cgatgagcca cccgaacgcc acccgggact gcccctcgag
      421 ggcgatgagg gcctggccca gggctacggc gagcatgaag gcggcgctgg agagggccag
      481 caggccgaga tccagccgcg tcaggacgaa gtcgtcgccg aacaggatgt cgatgaggaa
      541 gggcccgaga agggcggcgc cggccacccc ggcgacgccg atgaccccga cggcggccag
      601 gagacgccgc aggcggtccc ggaactcgtg gaagcggccg gcgccggcca aggcagagag
      661 cttcggcagg agggcggcct gcaccgcctg gaacaggaag accggcaccc gggagacggc
      721 gaggccggcg aggaagactc cggccttgtc cgactcggac tcgtcggcca gcagctccac
      781 ggccaggacc ccggcgtaga gcaggaaggc cgtcagcacc gaagcggcga gcaaggaacc
      841 cagggccgcg cccagctccc cccacggggc cggtggcccg tcggacgcga gatcgtgctc
      901 cccgagcaga gccaggacga tggcgacacc cggggccagc ccgaccgcca ctgcatacgg
      961 gccggcggtg tcgaccccga gcagagccag gacgatggcc gcggccaccc gcaggctgtt
     1021 ctcgtacacg aagaacaggg catagccgcg gaagcgaccg agcccggcca gggtgccgcg
     1081 ggcgagatgg accgcggagt agccgaggat ggccagcacg aacccgatca ggatgaggac
     1141 ctggtcgtcg aagaggtggt ccaggaggag cggggcggcg accagggtgg tcaggaccag
     1201 ggccccgatg agcgcggtcc cgagcaggcc cgcctgtttg acgagggggc cgaccccgac
     1261 gccccgggcc cagcggttgg ccagcgaacg gctgacctcc tgctccagcg gcaggaagaa
     1321 cccgggaccg acgaggtaga tcaacgccca cagcaccgag agcggcccga aggcgtcgac
     1381 gcccagcgtc cgggccgcga tgcccaggaa cacgtaggac atgaccccgt tgacgacgag
     1441 gccaccgccg accgtcagcg tcccgtcggg cagcgggttc cgctcggcga gcgcggtgaa
     1501 ccgggcccgc gtcacaccgg ccgtcacgtc gtcacgctct cggcggcggc tcggatgtca
     1561 atggtcctca ggccgcgccg tcgcggccga ccgccgtcgc ccggtcgtcg aggacgccgt
     1621 ccacccggtc gacccccatc gacgcgccct gctcgacgac ctcctcgtcc ttgctgcccg
     1681 tgggcatggc ccactggccg ccgaggtcca ggtagccctc ggcgtcgttg gcgctgccgg
     1741 gctgcacccg cagctcgaac tcgcgctcgc gctggtcgaa gatgtgcagg ttgttctcgt
     1801 cgtagacgcc ggccgtgagg tggtaggtcc ccggggtcag gtgcatgcgg gggatcttgt
     1861 agaagatcac gccctccccg tcgaccatgc cggtgttgag ccggccccga cggctgttcg
     1921 gggccgtgat catcgtcccc gagtcggtgt ggaagcgcac ggcgaagctg gggttcttga
     1981 tcggctggtg ggccttgtag tggatgcgca cgaccagcgg acgaccggag ttggcgaacg
     2041 gcgtgaccgc cctgtgggcg ttcacgaagc tcacgtgctc gatggtgatc tccccggtgc
     2101 cgcggcgctc gcctcgtccc tcgaggatca ggtcggcgtc gcccagatcc ccctcgaagc
     2161 cggcctcggc ctcggtgatg ttcacctgct gcagatagct gcgggcgatg tcgatggcgt
     2221 ggccctgctc gcggacctcg ccccgctcca tccagatggc ctggtcgcag agggactgga
     2281 cctggccgag gctgtgggtc acgagcacga tggtca
//
LOCUS       DASTFV010000506         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1878580, whole genome shotgun sequence.
ACCESSION   DASTFV010000506 DASTFV010000000
VERSION     DASTFV010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878580"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VFP02_09245"
     CDS             <1..474
                     /locus_tag="VFP02_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9543255.1"
                     /translation="LAIVDVTPGVDHAKAEPIVTFVSGPETFASFQEILDRTVEFNPT
                     RSVASLRRGILHNAKENPDGSWTWRWDAMREWNFPEGEMPDFDSLWEKVDQITAPILF
                     LRGGAAGSVVGDEDQAKLVRRQPTTQVEVVADAGHSLQGDQPLELARLLSQFQPS"
     gene            complement(585..1502)
                     /locus_tag="VFP02_09250"
     CDS             complement(585..1502)
                     /locus_tag="VFP02_09250"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HET9543256.1"
                     /translation="MPTLLSAVAALAGRQHGLVTTPQVMDLGGTRHVITHMVACGQWD
                     RVTPMLLRRVGSPRSRGQRVMAAVLDAGEGAGLSHRSAAAWWRLRGFLLADIEVMRLR
                     SMNSIPPRLARTIHEPRCLPAHHLTVLDWVPITVPSRIPFDLAATQPWLAAKALDRAW
                     AANLLTHTSSTLMLDDLAERGRKGITLMRELLAERGPDYRPNDTNLEDRFQELAREAG
                     FYDLARQRNLLDREWLGRVDFVSAKRMLVIEVDSAFCHGALVDEADDLVRTEALTAAG
                     YRVERFTDNEVWFDPKGTVTRLRTIRSGT"
     gene            complement(1613..2050)
                     /locus_tag="VFP02_09255"
     CDS             complement(1613..2050)
                     /locus_tag="VFP02_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9543257.1"
                     /translation="MAAHPAELAHRYAVALDRKDWDALVGLFVPAIQDKVRASLVPAV
                     ERLGVTVLSVTTQTVDLVDDDHATGVVYCTGEIEDPDEGWIRQQIVYQDRYERVDGQW
                     YIRARDHQLVYGLPLPESPLQQPPADWPTHQTGRGTYPPRSGL"
     gene            complement(2031..>2847)
                     /locus_tag="VFP02_09260"
     CDS             complement(2031..>2847)
                     /locus_tag="VFP02_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET9543258.1"
                     /translation="VDRAVEILDFLTDNPRDAFSLSELARRLDLNKASAHAILNALTD
                     QGYLLRHPTRKDYRLGPRLIAVGEAAREQYPVVEFARDELRRLSEDLGTECVASAPIG
                     NEMVIVDRAGPVRPIGLTVAVGYRFPLVPPMGTVFMAWSSPEEIDEWLRRVGPDVTEA
                     ELDRYRLALGAVRGRGYSAGLDADGSRSLAEAQAGDLDGDEYQLIEMGGAEEYRVTTI
                     GAPVFDTEGAVALGVFILGFRDALTAEQVPDVAERLVKATGAITESIHGRPPR"
BASE COUNT          434 a          957 c         1027 g          429 t
ORIGIN      
        1 ctggccatcg tggacgtcac ccccggcgtc gaccacgcca aggccgagcc catcgtcacc
       61 ttcgtgtcag gacccgagac cttcgcctcg ttccaggaga tcctggaccg cacggtcgag
      121 ttcaacccga cccggtcggt cgcctcgctg cgccggggga tcctgcacaa cgcgaaggag
      181 aaccccgacg gcagctggac ctggcggtgg gacgccatgc gggagtggaa cttccccgaa
      241 ggcgagatgc ccgacttcga ctctctctgg gagaaggtcg accagatcac ggcgccgatc
      301 ctgttcctgc ggggcggggc ggccggcagc gtcgtcggcg acgaggacca ggccaagctc
      361 gtccgccgcc agcccaccac ccaggtcgag gtcgtcgccg acgccggcca cagcctccaa
      421 ggcgaccaac ccctcgagct ggcccgcctc ctcagccagt tccaaccctc ctgacccgcc
      481 ctccccgttc tggcctccga tcctccgtct atgaccggca gatcagaggc cagaacgaga
      541 ggctgttgat tctggcctcc gatcctccgc ctatcaccgg aggatcaggt gccagaacgg
      601 atggtgcgga ggcgggtgac ggtgcccttc gggtcgaacc agacctcgtt gtccgtgaat
      661 cgctcgacgc ggtaaccggc ggccgtcagc gcctcggtgc ggacgaggtc gtcggcctcg
      721 tcgacgaggg caccgtgaca gaaggcgctg tcgacctcga tgaccagcat tcgcttggcg
      781 ctgacgaagt cgactcggcc cagccattca cggtcgagga ggttgcgctg gcgggcgagg
      841 tcgtagaagc cggcttcccg agccagttcc tggaagcggt cctcgaggtt cgtgtcgttc
      901 ggccggtagt cgggtccgcg ctcggccagc agctcgcgca tgagcgtgat gccttttcgc
      961 cctcgctcgg ccagatcgtc gagcatcagc gtcgagctcg tgtgggtgag gaggttcgcg
     1021 gcccaggccc ggtcgagggc cttggccgcc agccatggct gggtggcggc gaggtcgaag
     1081 gggattcgtg acggaaccgt gatggggacc cagtcgagca cggtcaggtg gtgagcagga
     1141 aggcaccggg gctcgtgaat ggtgcgggcc aggcgtggcg ggatcgagtt catcgaccga
     1201 aggcgcatca cctcgatgtc ggcgaggagg aagccgcgca ggcgccacca tgcggcggcc
     1261 gaccgatgtg agagtccggc tccctcaccg gcatccagca ccgccgccat gacgcgctga
     1321 ccacgagatc gagggctacc caccctgcgg aggagcatcg gcgtgacccg gtcccactgt
     1381 ccgcaagcga ccatgtgggt gatcacgtgt cgagtaccgc cgaggtccat gacctgcggc
     1441 gtggtgacga ggccgtgttg gcgcccagcg agcgcggcga cggccgagag aagagtgggc
     1501 atgagcttcc ccagacgcgg gatttcgtcg gcgggggaag cgcaggagcg accgtacatc
     1561 tcggccgaac gtcggccaac tggcctctga tcctccggtc gtgaccggag gatcagaggc
     1621 cagaacgagg ggggtaggtg ccgcggccgg tttggtgggt gggccagtcg gccggtggtt
     1681 gttgcagggg gctctccggc aacggcaggc cgtagacgag ctggtggtcc ctggccctga
     1741 tgtaccactg gccgtcgacc cgttcgtagc ggtcctggta gacgatctgc tggcggatcc
     1801 agccctcgtc ggggtcctcg atctcgcccg tgcagtagac gacaccggtg gcgtggtcgt
     1861 cgtcgacgag gtcgacggtc tgggtggtca cggacagcac ggtcacgccg aggcgctcga
     1921 cggcgggcac cagcgaggcc cggaccttgt cctggatcgc cggcacgaac agcccgacga
     1981 gggcgtccca gtccttgcgg tccagcgcca ccgcgtagcg gtgggccagc tcagcggggt
     2041 gggcggccat ggatggactc ggtgatggcg cccgtggcct tgaccaggcg ctcggcgacg
     2101 tcggggacct gctcggcggt cagggcatcc cggaagccga ggatgaagac cccgagggcc
     2161 acggcgccct cggtgtcgaa caccggggcg ccgatggtgg tgacccggta ctcctcggcg
     2221 ccgcccatct cgatgagctg gtattcgtcg ccgtcgaggt cgccggcctg ggcctccgcc
     2281 aaagagcggc tgccgtcggc gtccagcccc gccgagtatc cccgaccccg cacggcaccc
     2341 agcgccaggc ggtagcggtc cagctcggcc tcggtcacgt cggggcccac tcggcgcagc
     2401 cactcgtcga tctcctccgg ggacgaccac gccatgaaga ccgtccccat cggcgggacc
     2461 agcggaaagc ggtagccgac ggccaccgtg agcccgatgg gcctcaccgg cccggcccgg
     2521 tcgacgatga ccatctcgtt gccgatcggt gcgctggcga cgcactcggt cccgaggtcc
     2581 tcgctgaggc ggcgcagctc gtcccgggcg aactccacga ccgggtactg ctcccgggcc
     2641 gcctcgccca cggcgatcag gcggggcccc agccggtagt ccttccgggt ggggtgacgc
     2701 agcaggtagc cctggtcggt gagggcgttg aggatggcgt gggcgctggc cttgttcagg
     2761 tcgaggcggc gagccagctc ggacagcgag aaggcgtccc gcgggttgtc cgtcaggaag
     2821 tcgaggatct cgacggcccg gtcgacc
//
LOCUS       DASTFV010000507         4765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1879649, whole genome shotgun sequence.
ACCESSION   DASTFV010000507 DASTFV010000000
VERSION     DASTFV010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879649"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VFP02_09265"
     CDS             complement(<1..389)
                     /locus_tag="VFP02_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9543259.1"
                     /translation="MADQIPMIIGDERVASDESIEVRSPFDGSIVGTVAKGTEKHLDQ
                     AVAIAAARHRAGPLPAHERAAVLDRAARLLAERVEEFATVISAEAAKPIKTARVEAAR
                     AVDTVSFSAAVARTLGGELIPLDASSAG"
     gene            complement(463..927)
                     /locus_tag="VFP02_09270"
     CDS             complement(463..927)
                     /locus_tag="VFP02_09270"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF016040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HET9543260.1"
                     /translation="MREHVVRAEPLTSAGFAPFGQVIGADEVRLELREDETFHLDVLS
                     YDHQPLEFTRLNRHHRATQALVALNGKPTVVLVGPAEVDFSDPAHLDTLRAFLCDGSA
                     GVNLALGTWHAGPYPLTDHVDLVNVQGAQVMATDVEEAHLDQALDVVVTIRL"
     gene            964..1347
                     /locus_tag="VFP02_09275"
     CDS             964..1347
                     /locus_tag="VFP02_09275"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9543261.1"
                     /translation="MDVRSIEGVAPVVEHNGTVPVWWLVSPREMHDITVGGHLELVSE
                     FEVAGGGLVDPHSHPTHEFYYVTSGRGLMTIAGEEREIGQGDLVHIPPDAVHSLRPLS
                     DHAPIHCFCFAVGVKDAAEVDYTSH"
     gene            complement(1339..1968)
                     /locus_tag="VFP02_09280"
     CDS             complement(1339..1968)
                     /locus_tag="VFP02_09280"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543262.1"
                     /translation="MVNVDQLIVIGAATLGESGALVMPSRIRPAWTGATVTGRAFTVT
                     CAPGDNLAVHVATTMAPPGSVLVVDASAEPERGYWGEILTTAAETTGITGLVIDGGIR
                     DVAALSAHGFGAFSDRIALRGASKDQPGEVGGPVTVGDVTVHTGDTVVGDADGVVVIP
                     AGRFDEVVGAARHRSDREQDLFRRLQSGETTVELLGLDPTAIRQVDPSV"
     gene            complement(2034..2753)
                     /locus_tag="VFP02_09285"
     CDS             complement(2034..2753)
                     /locus_tag="VFP02_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET9543263.1"
                     /translation="MRALVVNPDGHCPAGMVGDRLEHHGFELDEVVPVADVTHPVSAT
                     PFPDPEDYDLIVPMGSPWSVYDTDRIGTWIGRELDLLRAAQAADIPVLGVCFGGQALA
                     AALGGTVEATPRPEFGWYQIESHDPDVLAPGPWFQWHFDRFTVPEGATILAHSDSGVQ
                     AFRTGRSLGVQFHPELTTDILEGWLADADEAELRHLDGTPEGLLAETAERQEQARPHT
                     DALVDHFLTTVAGLVPLSARH"
     gene            complement(2828..3310)
                     /locus_tag="VFP02_09290"
     CDS             complement(2828..3310)
                     /locus_tag="VFP02_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543264.1"
                     /translation="MPTSLRTAVAAAAIAGAGLAGFGVTSAFAQTDAGDDATTTTEAP
                     ADPAAQPGAPGCEGRGEGPALDAMAEAIGIEVEDLRAALEDGQTPAEVAEANDVSRAD
                     LVDAIVADITDHIEQGVEDGDLTQAEADERLADVEDHANDIVDGMGHGPGGPPPTSES
                     "
     gene            3465..4154
                     /locus_tag="VFP02_09295"
     CDS             3465..4154
                     /locus_tag="VFP02_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9543265.1"
                     /translation="MEGTPARVLVVEDDQNVRQTLDSALRFAGHEVVTVDDGDEALDE
                     ISARAPDLIVLDVMMPRLDGLTVCRMLRDRGYRTPVLVLTARHEVSDRVAGLDAGADD
                     YLAKPFALDELLARVRALLRRTSPSGSAAVLRVGDLVLDPRRRSAQRGDRPIELTKTE
                     FDLLELLMLNVGIVLERSVIYDRIWGYDFETGSKSLDVYVGYLRRKTEEADETRLVHT
                     VRGVGYVVREP"
     gene            4247..>4765
                     /locus_tag="VFP02_09300"
     CDS             4247..>4765
                     /locus_tag="VFP02_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543266.1"
                     /translation="MSLRWRLLAVFAVLGALAIGAANLTAWLSTSNELRGEVDDFLVQ
                     RGNEILEGRRQLPQGPGGFDNGGPRGTPSTFAPDVVTQVLDENGAIEQSVGQDLPVDD
                     ADRAVAAGTDDARLSTVTVDGVELRVLTVHLRGGGAVQIARELTETDDVLTALAGRLA
                     LITLTGAALAGLV"
BASE COUNT          654 a         1645 c         1756 g          710 t
ORIGIN      
        1 ccggccgagg aggcgtcgag cgggatcagc tcgcccccca gcgtccgggc cacggcggcg
       61 gagaagctca cggtgtcgac ggcgcgggcc gcctcgaccc gagccgtctt gatgggtttg
      121 gccgcctcgg cggagatcac ggtggcgaac tcctccaccc gctcggccag gagtcgggcc
      181 gcgcgatcga gcacggcggc ccgctcgtgg gccggcaagg gcccggcgcg gtgccgtgcg
      241 gcggcaatgg cgaccgcctg gtcgaggtgc ttctcggtgc ccttggccac cgtgcccacg
      301 atggacccgt cgaaggggga gcggacctcg atgctctcgt cggaggcgac acgctcgtcg
      361 ccgatgatca tcggaatctg gtcggccatc gggatcctcc gtgcggctcg tccggctcgg
      421 cgtcacggta gctgcgcccg ccgaaggccc ctgagggcgg gctcagagcc ggatggtcac
      481 cacgacgtcg agggcctggt ccaggtgggc ctcctcgacg tcggtggcca tgacctgggc
      541 cccctgcacg ttgaccaggt cgacatggtc ggtcagcgga tagggcccgg cgtgccaggt
      601 gccgagggcg aggttgacgc ccgcgctccc gtcgcagagg aaggcccgca gcgtgtcgag
      661 gtgggccggg tcggagaagt cgacctcggc ggggcccacc aggaccaccg tgggcttccc
      721 gttcagggcg accagcgcct gcgtggcccg gtggtgccgg ttgagcctcg tgaactcgag
      781 gggttggtgg tcgtagctca ggacgtcgag gtggaaggtc tcgtcctccc gcagctccag
      841 ccggacctcg tcggccccga tgacctggcc gaagggggcg aacccggccg aggtgagcgg
      901 ctcggctcgg accacgtgct cgcgcatcgc tctagtctga cgcggtcgag cagggggtga
      961 cgggtggacg ttcggtcgat cgaaggcgtg gccccggtgg tggagcacaa cggcacggtg
     1021 ccggtgtggt ggctggtctc gccccgcgag atgcatgaca tcaccgtcgg cggtcacctg
     1081 gagctggtgt cggagttcga ggtggcgggt ggcggcctgg tcgaccccca ctcgcacccc
     1141 acccacgagt tctattacgt gacctccggc cgggggctca tgacgatcgc cggcgaggag
     1201 cgggagatcg gccagggtga cctcgtgcac atcccacccg atgcggtgca cagcctgcgc
     1261 ccgttgagcg accacgcccc gatccactgc ttctgcttcg ccgtcggggt caaggacgcc
     1321 gccgaggtcg actacacctc acactgacgg gtcgacctgc cggatcgccg tcgggtcgag
     1381 gccgagcagc tcgacggtcg tctcgcccga ctggagccgg cggaagaggt cctgttcccg
     1441 gtcggaccgg tgacgggccg cgcccactac ctcgtcgaac cgcccagcgg ggatgaccac
     1501 gacaccgtcc gcgtccccga cgacggtgtc gccggtgtgc acggtcacgt cgccgacggt
     1561 caccggcccg cccacctcgc ccggttgatc cttgctcgcc ccccgcaggg cgatgcggtc
     1621 actgaaggcg ccgaagccgt gggccgacag cgcggcgacg tcgcggatgc caccgtcgat
     1681 gacgagcccg gtgatgcccg tggtctcggc ggccgtggtc aggatctcgc cccaatagcc
     1741 ccgctctggc tcggccgaag cgtccacgac cagcacgctt ccgggcggcg ccatggtggt
     1801 ggcgacgtgg acggccaggt tgtcgcccgg cgcgcaggtc accgtgaagg cccggccggt
     1861 cacggtggcg ccggtccagg ccggacggat gcgggacggc atgacgaggg ccccgctctc
     1921 gccgagggtg gctgctccga tcacgatgag ctggtcgacg ttcaccaccc gccgacccta
     1981 ccggccgctt ctggcgccct tctctcgagg ctctgccgct acgaatccga ccgctagtgc
     2041 ctcgctgaca gggggacgag gccggcgaca gtggttagga agtggtcgac gagggcgtcg
     2101 gtgtgcgggc gggcctgttc ctggcgctcg gcggtctcgg ccaggaggcc ttcgggcgtg
     2161 ccatcgaggt ggcggagctc ggcctcgtcg gcatcggcca gccaaccctc gaggatgtcg
     2221 gtggtcagct cgggatggaa ctgcacgccg aggctgcgac ccgtccggaa cgcctgcacg
     2281 cccgagtcgc tgtgggccag gatcgtggcc ccctcgggca cggtgaaccg gtcgaagtgc
     2341 cactggaacc agggcccggg cgccagcaca tcggggtcgt gggactcgat ctggtaccag
     2401 ccgaactcgg ggcggggggt ggcctcgacc gtgcccccca gcgcggcggc cagggcctgg
     2461 cccccgaagc acacgccgag caccgggatg tcggcggcct gggcggcgcg gagcaggtcc
     2521 agctcccgac cgatccaggt cccgatccga tccgtgtcat agaccgacca gggcgagccc
     2581 atggggacga tgaggtcgta gtcctcggga tcggggaacg gcgtggcgga gaccgggtgg
     2641 gtgacgtcgg cgacgggcac cacctcgtcc agctcgaagc cgtggtgctc gaggcgatcg
     2701 cccaccatcc ccgcgggaca gtgcccgtcc gggttcacca ccaacgcccg catgcgagcg
     2761 aggctaccgc tcaccgaaat tgcgtcagct ggtcacgccg gtggtgacca actgacgcaa
     2821 tctccggtca ggactcagac gtcggcgggg gaccgcccgg gccgtggccc atgccgtcga
     2881 cgatgtcgtt ggcgtggtct tcgacgtcgg cgaggcgctc atcggcctcg gcctgggtca
     2941 ggtcaccgtc ctcgacgccc tgctcgatgt ggtcggtgat gtcggccacg atggcgtcca
     3001 ccaggtcggc ccggctgacg tcgttggctt ccgcgacctc ggccggggtc tgaccgtcct
     3061 cgagggcggc gcggaggtcc tcgacctcga tgccgattgc ctcggccatg gcgtcgagcg
     3121 ccgggccctc gccacggccc tcacagccgg gggcaccggg ctgggcggcc ggatcggccg
     3181 gggcctcggt ggtcgtggtc gcgtcgtcac ccgcgtcggt ctgggcgaag gcactggtga
     3241 cgccgaagcc ggcgaggccg gctccggcga tggccgccgc ggcaaccgcg gtacggaggg
     3301 aggtgggcat gatcagggct cctttcgtgc tcttgtcagg gttgacggaa accacgatcc
     3361 cggcgccggg taagcggggg ttcgcgctgg ggctgagaac cacgcgagaa aggccttcgg
     3421 gcgttctcac ctggttcttg ctttcgggca ctagaccgac gccggtggaa ggaacaccgg
     3481 cccgggtcct cgtcgtcgag gacgaccaga acgtgcgtca gacccttgac tcggccttgc
     3541 gcttcgccgg ccacgaggtc gtcacggtgg atgacggcga cgaggccctg gacgagatca
     3601 gcgcccgcgc acccgacctg atcgtgctcg acgtgatgat gccgaggctc gacggcctca
     3661 ccgtctgccg catgctgcgg gatcggggtt accggacgcc ggtgctggtc ctgaccgctc
     3721 gtcacgaggt gtccgaccgg gtagccggac tcgacgcggg ggccgacgac tacctggcca
     3781 agcccttcgc cttggacgag ctgctggccc gcgtccgggc cctcctacgg cggaccagcc
     3841 cgagtggcag cgccgccgtc ctccgggtgg gcgatctggt gctcgatccc cgacggcggt
     3901 cggcccaacg cggcgaccgg ccgatcgagc tgaccaagac cgagttcgac ctgctcgagc
     3961 tgctgatgct caacgtcggc atcgtcttgg agcgcagcgt catctacgac cgcatctggg
     4021 gctacgactt cgagaccggg tccaagtcgc tggacgtcta cgtgggctac ctgcgacgca
     4081 agaccgagga ggccgacgag acccgcctgg tccacacggt ccgcggcgtc ggctacgtcg
     4141 tccgggagcc ctgacgcatg cgccccgccc caacggttct gtttgcctct ccccgcggct
     4201 ggacgcggct gggtgcaacc agaacgggcg ggagccctga gcagcgatga gcttgcggtg
     4261 gcggttgttg gcggtcttcg ccgtgctggg ggcgctggcc atcggggcgg ccaacctcac
     4321 ggcgtggctg tcgaccagca acgagctgcg gggtgaggtc gacgacttcc tggtccagcg
     4381 gggcaatgag atcctcgaag gtcgccgcca gctcccccag gggcccgggg gcttcgacaa
     4441 cgggggcccg agaggcaccc cgtccacctt cgcccccgat gtcgtcaccc aggtcctcga
     4501 cgagaacggt gcgatcgagc agtccgtggg ccaggacctg cccgtcgacg acgccgaccg
     4561 ggccgtggcc gcggggaccg acgacgcccg gctgagcacg gtgaccgtcg acggggtcga
     4621 gctccgcgtc ttgaccgtgc acctccgagg gggcggtgcc gtgcagatcg cccgggagct
     4681 gaccgagacc gacgacgtcc tcaccgccct ggccgggcgc ctcgcgttga tcaccttgac
     4741 cggagcggca ctggccggcc tcgtc
//
LOCUS       DASTFV010000508         6359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1880310, whole genome shotgun sequence.
ACCESSION   DASTFV010000508 DASTFV010000000
VERSION     DASTFV010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880310"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFP02_09305"
     CDS             <1..322
                     /locus_tag="VFP02_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9543267.1"
                     /translation="GLLSGGERQMLAMGRALMMEPSVLLLDEPSAGLSPVLQTEVFER
                     ADQINEAGVSIVMVEQNARRCLQVCDRGYVLDQGQNAYTDSGDALLHDPKVIELYLGT
                     LAKA"
     gene            complement(343..1674)
                     /locus_tag="VFP02_09310"
     CDS             complement(343..1674)
                     /locus_tag="VFP02_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9543268.1"
                     /translation="MRRLGYWVVDQVVDHISGIGDGPALRTDSPDRLLALLGGPVPTE
                     PGEPQAAMATLVEVALATMQHTDHPRFFTRVPGPSSYPAVLGEWLGTGFQAIAASWGG
                     GAGPTAVELVVLDWLRTLLRLPDGTEGILLSGGSMANLTGLMAARHEVGDGVVYLSDQ
                     THSSIGRGLAATGFAEIRLLPTDDRLRLGVDAVLRAVADDRAAGHRPAIVVATAGTTN
                     TGAVDPLPELADLCAAEGLWLHVDGAYGAPAALAPSGCSVLAGLERADSVVLDPHKWL
                     FQPYDIGVLLVRRPGALERAFTMNPEYLADIVADTDEVDLRSRSLELSRRARALKLWL
                     TFRTYGSERIGQAIERGIASAEFVASVLAADPAWEIVTPAQLGIITFARVGASDEQHV
                     RAAADLTEDGFAAVSTTTLRGRAVLRLCTINPRTTEADLDETLTRLADLIS"
     gene            complement(1712..2887)
                     /locus_tag="VFP02_09315"
     CDS             complement(1712..2887)
                     /locus_tag="VFP02_09315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9543269.1"
                     /translation="MIGAALPTVAEVVIIGGGVMGTSVAFHLAEAGVADVLLLERDAL
                     GAGSTAKAAGGVRAQFSDAVNIALGARGLEAFEGFAERPGGAIDLDQVGYLFCLTTVE
                     DLATFEAAVALQNSMGVATRLLAPTEAAGLAPVLRTDDLLAAAFHARDGICSPDGVVQ
                     GYAAAARRLGATLLTGVEVTAIEADASGVAAVVTGAGRVTTGSVICVAGAWSEALAAT
                     AGVHLPVTPLRRQIVVTEALDERRQALAPRSTPMTIDAASTFYFHREGPGMLMGLSYE
                     DEVPGFDLSTDDAWLPTLLAAVEARAPALADLGVAHRWAGLYEVSPDHNGLIGAATDR
                     PGFFYATGFSGHGFLQGPAVGEVLRDLWLGREPFVDVSPLSAERFAVGAVRPEHHIV"
     gene            complement(2884..4431)
                     /locus_tag="VFP02_09320"
     CDS             complement(2884..4431)
                     /locus_tag="VFP02_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9543270.1"
                     /translation="MPNDASILLQHTTDDLARSAQRALERCGVDLAIGDGPTITARTP
                     VTGEELFAVPAVDRAGMDAAIGRAHATFLEWRTVPAPVRGALVKRFGVLLGEHHQDLA
                     DLVAIEVGKIGAEARGEVQEMIDICDFAVGLSRQLDGRTMPSERPGHRLMETWHPLGV
                     VAVISAFNFPVAVWSWNAALALVCGDTVVWKPSESTPLTAMACSAILDRAAADVGAPG
                     DLNVLAVAGPDDAAPLLDDPRVALVSATGSERMGALVAPRVAARFGRCLLELGGNNAA
                     VVTPSADLDLAVRGIVFAAAGTAGQRCTTLRRVIAHESLVDDLVGRLAEVFGRLPVGD
                     PFADGTLVGPLIAPGAADAMRAALDQAVDEGGEVVAGGEPLTADEAPDAVYVRPALVR
                     MPAQTDVVRRETFAPVLYVLTYRSLEEAVALHNDVPQGLSSSIFTTDQREAERFLAAD
                     GSDCGIVNVNIGTSGAEIGGAFGGEKNTGGGRESGSDAWRGYMRRTTNTVNYSDELPL
                     AQGVTFT"
     gene            complement(4464..5198)
                     /locus_tag="VFP02_09325"
     CDS             complement(4464..5198)
                     /locus_tag="VFP02_09325"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HET9543271.1"
                     /translation="MTLTMPGESRQYRQARDALLEQEIDLRRQMEAVAAARRALPPGG
                     EVPEDYVFEEQADDGTVAPVRLSELMGEGRSVAVYNYMFPRHVGDDRPGPTEGESAAL
                     PLEEGPCPSCTALLDQLDAAARHVAPHADLVVSAKAPIERLTTFARERGWRHLRLVSS
                     AGNSYARDYGGEVDGQQQPMMNVFERDGAVIRHFWGSEMLYAPSDPGQDPRHMGTLEP
                     SWNLFDLMRPGRGEDWDEQFDYDCCR"
     gene            complement(5313..>6359)
                     /locus_tag="VFP02_09330"
     CDS             complement(5313..>6359)
                     /locus_tag="VFP02_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9543272.1"
                     /translation="GGVLGADVEYSPGDSGDTNTDIANQTTDRLLSEGVDVIVGAASS
                     GVSFTVIDKITGANVIQFSPANTSPDFTDYDDNGLYFRTAPSDVLQGRVLSDLIVADG
                     NATVGIMARQDPYGEGLLQYTAGPLTEAGAEVTNDGGIVYDPAAENFDAEVGEIVAAD
                     PDAFVMIGFEESAQILTSLFEAGYTQGDKDIYLVDGNVGNALGENFADNPGILEGVRG
                     TLPSAELSEDLRGRLLEVDPELVDFSYGPETYDAVTITALAAEVAGTDDPVAIAAEIN
                     GVTRDGTTCTTFAECRDLVADGEDIDYDGAGGPYTFGDAGEPTEASFAILQYGPDNQI
                     DDSLTEYQFAAIEE"
BASE COUNT          908 a         2216 c         2370 g          865 t
ORIGIN      
        1 cgggctgctg tcgggtggtg agcgtcagat gctggccatg ggccgggccc tgatgatgga
       61 gccgtcggtc ctcctgctgg acgagccctc cgccggtctc agccccgtcc tgcagaccga
      121 ggtgttcgag cgggccgacc agatcaacga ggccggcgtg tcgatcgtca tggtcgagca
      181 gaacgcccgc cggtgcctgc aggtctgtga ccggggctac gtcctcgacc agggccagaa
      241 cgcctacacc gactcgggcg acgccctcct ccacgacccc aaggtcatcg agctctacct
      301 cggcaccctg gccaaggcct agcccggccc agtcgagggt ggtcaactga tcaggtcggc
      361 gagccgggtg agggtttcat cgaggtctgc ttcggtcgtt cgcgggttga tcgtgcacag
      421 gcggagcacg gcccggcccc gcagcgtggt ggtggagacg gcggcgaacc cgtcctcggt
      481 gagatcggcg gcggcgcgga cgtgctgctc gtcggaggct cccacccggg cgaaggtgat
      541 gatgcccagc tgagccggcg tcacgatctc ccacgcggga tcagcggcga gcacggacgc
      601 gacgaactcc gcggaggcga tgccccgctc gatggcctga ccgatgcgct cgctgccgta
      661 ggtccggaag gtcagccaga gcttgagggc ccgggctcgc cggctcagct ccagtgaccg
      721 gctgcgcagg tcgacctcgt cggtgtcggc gacgatgtcg gccaggtact ccgggttcat
      781 ggtgaaggcc cgctccaggg cgcccggccg tcggaccagg agcaccccga tgtcgtaggg
      841 ctggaagagc cacttgtggg ggtccagcac caccgagtcg gcccgttcca gcccggccag
      901 caccgagcat ccggacggcg ccagggcggc gggggcccca taggcgccgt cgacatggag
      961 ccagaggccc tcggcggcgc agagatcggc cagctcgggg agggggtcga cggccccggt
     1021 gttggtggtc cccgcggtgg ccacgacgat cgccggccgg tgcccggcgg cccggtcgtc
     1081 ggccacggcc cggaggacgg cgtcgacgcc cagccggagg cgatcatcgg tcgggagcag
     1141 tcggatctcg gcgaacccgg tcgcggccag gccgcggccg atggacgagt gcgtctggtc
     1201 ggacaggtag acgaccccgt cgcccacctc gtggcgggcg gccatcaggc cggtcagatt
     1261 ggccatcgat cccccggaca gcaggatgcc ctcggtgccg tccgggaggc ggaggagggt
     1321 gcgcagccag tccagcacga ccagctcgac cgccgtgggc ccggcccccc cgccccagga
     1381 ggcggcgatg gcctggaacc cggtgcccag ccactcgccg agcacggcgg ggtacgacga
     1441 cggcccgggg acgcgggtga agaagcgggg atgatcggtg tgctgcatgg tggcgagggc
     1501 cacctcgacc aacgtggcca tggccgcttg cggctctccg ggctcggtgg ggaccggccc
     1561 gcccaggagg gccaggagcc ggtcgggcga gtcggtgcgc agcgcgggac cgtcgccgat
     1621 cccgctgatg tggtcgacca cctggtcgac gacccagtag ccgagccgcc gcatctcctc
     1681 ggaaccgaag ccgagcgggt ccggatcggc gtcagacaat gtggtgctcc ggtcggacgg
     1741 cgccgacggc gaaccgctcg gcggacagcg ggctgacgtc cacgaacggc tcccgcccca
     1801 gccacaggtc ccggagcacc tcgcccaccg ccggtccctg gaggaagccg tggccggaga
     1861 accccgtggc gtagaagaac ccgggccggt cggtcgctgc tccgatgagc ccgttgtggt
     1921 cgggggagac ctcgtagagc ccggcccacc ggtgggccac acccaggtcg gccagggcgg
     1981 gggcccgggc ctcgacggcg gcgaggaggg tggggagcca ggcgtcgtcg gtcgagaggt
     2041 cgaagccggg cacctcgtcc tcgtaggaga ggcccatgag catgcccggc ccctcccggt
     2101 ggaagtagaa ggtggaggcg gcgtcgatgg tcatcggggt gctgcggggc gccagggcct
     2161 gccgtcgctc gtccaacgcc tcggtgacca cgatctgccg gcggagcggg gtgacgggca
     2221 ggtggacccc ggccgtggcg gccagggcct cggaccaggc cccggcgacg cagatgaccg
     2281 agccggtcgt gacccggccg gcgccggtca cgaccgccgc gacccccgag gcgtcggcct
     2341 cgatggcggt gacctcgacc cccgtcagga gcgtggcgcc caggcgccgg gccgccgcgg
     2401 cgtagccctg gaccacaccg tcgggcgagc agatgccgtc ccgggcgtgg aacgccgccg
     2461 ccaggaggtc gtcggtgcgc agcaccgggg ccaggccggc cgcctcggtc ggggccagca
     2521 ggcgggtggc cacgcccatc gagttctgca gggccaccgc ggcttcgaag gtggccaggt
     2581 cctcgaccgt ggtcaggcag aacaggtacc cgacctggtc caggtcgatg gccccgcccg
     2641 gccgctcggc gaagccctcg aaggcctcca ggccccgggc tccgagggcg atgttgacgg
     2701 cgtcggagaa ctgggcccgg accccgcccg ccgccttcgc cgtggagccc gcccccagcg
     2761 cgtcccgttc gaggaggagg acgtcggcga cccccgcttc ggccaggtgg aaggcgaccg
     2821 acgtgcccat gactccgcca ccgatgatca ccacctcggc cacagtcggc agggccgccc
     2881 cgatcacgtg aaggtgaccc cctgggccag tggcagctcg tcggagtagt tgacggtgtt
     2941 ggtggtccgg cgcatgtagc cccgccaggc gtcggagccg gactcccgtc cgccgccggt
     3001 gttcttctcg ccgccgaagg cgcccccgat ctcggcaccg gaggtcccga tgttgacgtt
     3061 gacgatgccg cagtcggagc cgtcggcggc caggaagcgc tcggcctccc gctggtccgt
     3121 ggtgaagatg gacgaggaca ggccctgagg gacgtcgttg tggagggcca ccgcctcctc
     3181 caggctgcgg taggtgagga cgtagagcac cggggcgaac gtctcccgcc ggacgacgtc
     3241 ggtctgggcc ggcatccgca ccagggcggg ccggacgtag acggcgtcgg gcgcctcgtc
     3301 ggccgtcagc ggctctccgc cggccacgac ctcgcccccc tcgtccaccg cctggtcgag
     3361 ggcggcccgc atggcgtcgg ctgcccccgg cgcgatcagc ggcccgacca gggttccgtc
     3421 ggcgaagggg tcgccgaccg ggagccgtcc gaacacctcg gccagccggc cgaccaggtc
     3481 gtcgaccagc gactcgtggg cgatgacccg gcgcagggtc gtgcagcgct ggcccgccgt
     3541 gcccgcggcg gcgaacacga tgccccgcac ggccaggtcg aggtcggccg agggcgtgac
     3601 cacggcggcg ttgttgccgc cgagctcgag caggcagcgg ccgaagcggg ccgccacacg
     3661 gggggcgacg agggccccca tgcgctccga accggtggcg ctgaccagcg ccacccgcgg
     3721 gtcgtcgagc agcggggcgg cgtcgtcggg accggcgacg gcgagcacgt tcaggtcgcc
     3781 cggcgcgccg acgtcggcgg ccgcccggtc caggatggcg gaacacgcca tcgcggtcaa
     3841 cggggtggac tccgacggct tccagaccac cgtgtcaccg cagacgaggg ccagggctgc
     3901 gttccacgac cagacggcga ccgggaagtt gaaggcgctg atgacggcca ccacgccaag
     3961 ggggtgccag gtctccatca gccggtgccc cggccgctcc gagggcatcg tgcgaccgtc
     4021 gagctggcgg gagagaccga cggcgaagtc gcagatgtcg atcatctcct ggacctcgcc
     4081 ccgggcctcg gcgccgatct tgccgacctc gatggccacc aggtcggcga ggtcctgatg
     4141 gtgctcgccc agcaggaccc cgaagcgctt caccagcgca ccccgcacgg gagcgggcac
     4201 ggtgcgccat tcgaggaagg tggcgtgggc ccgaccgatg gcggcgtcca tgccggcccg
     4261 gtcgacggcc ggcacggcga agagctcctc gccggtgacc ggcgtccggg ccgtgatcgt
     4321 cggtccgtcg ccgatcgcca gatccacccc gcaccgctcc agggcacgtt gggccgagcg
     4381 ggccaggtcg tcagtggtgt gctggagcag gatcgacgcg tcgttgggca ccgctgtcgt
     4441 ctaccagatc cggaccggga ggttcagcgg cagcagtcgt agtcgaactg ctcgtcccag
     4501 tcctcgcccc gcccgggccg catcaggtcg aacaggttcc acgacggttc gagggtgccc
     4561 atgtggcgtg ggtcctggcc cgggtcggag ggggcgtaca gcatctcgga tccccagaag
     4621 tgccggatca cggcgccgtc gcgctcgaac acgttcatca tcggctgctg ctggccgtcg
     4681 acctcgccgc cgtagtcccg ggcgtaggag tttcccgcgg acgacacgag gcggaggtgg
     4741 cgccaacccc gttcccgagc gaaggtcgtc agccgctcga tcggcgcctt cgccgacacg
     4801 acgaggtcgg cgtggggcgc cacgtgacgg gcagcggcgt cgagctggtc gagcagggcg
     4861 gtgcaggacg ggcacgggcc ctcctcgagc ggcagtgccg cgctctcacc ctcggtgggc
     4921 ccgggccggt cgtcgccgac gtgacgcggg aacatgtagt tgtagaccgc gaccgaccga
     4981 ccctcgccca tgagctcgga gagccggacc ggggcgacgg tcccgtcgtc ggcttgctcc
     5041 tcgaagacgt agtcctcggg gacctcaccg ccgggcggga gcgcccgacg ggcggcggcc
     5101 acggcctcca tctgtcgacg gaggtcgatc tcctgctcga ggagcgcgtc gcgggcctgc
     5161 cggtactggc gggattcgcc gggcatggtg agggtcatgg tggttcgacc tccgccggcc
     5221 ggggatctca tcgcgcgggt cgtgacccgg acgcgaacga gccgcccctc tcggggcggc
     5281 tcgcacgctg ttgtccggtt gtaccgaggg gtctattcct cgatcgccgc gaactggtac
     5341 tcggtcaggc tgtcatcgat ctggttgtcg gggccgtact ggaggatcgc gaagctcgcc
     5401 tccgtgggct caccggcatc accgaaggtg tagggaccac ccgcaccgtc gtaatcgatg
     5461 tcctcgccgt ccgccacgag atcacggcac tccgcgaagg tggtgcacgt ggtgccgtcg
     5521 cgggtcaccc cgttgatctc agccgcaatg gcgaccggat cgtcggtccc ggccacctca
     5581 gcggccaggg cggtgatggt gacggcgtcg taggtctcgg ggccatagga gaagtccacc
     5641 agctccgggt ccacctcgag gaggcggcca cgcaggtcct cggagagctc ggccgacggc
     5701 agggtgcccc gcacgccttc caggatcccc gggttgtcgg cgaagttctc gccgagcgcg
     5761 ttaccgacgt tgccgtcgac caggtagatg tccttgtcgc cctgcgtgta gcccgcctcg
     5821 aacagcgagg tcaggatctg cgcggactcc tcgaaaccga tcatgacgaa ggcgtcaggg
     5881 tcggcggcga cgatctcgcc cacctcggcg tcgaagttct cggccgcggg gtcatagacg
     5941 atcccgccgt cgttggtgac ttcggccccg gcctcggtca gaggccctgc cgtgtactgc
     6001 agcagaccct caccgtaggg atcctggcga gccatgatcc cgaccgtggc gttgccgtca
     6061 gcgacgatca ggtccgagag gacccggccc tggagcacgt cggacggggc ggtacggaag
     6121 tacagcccgt tgtcgtcgta gtccgtgaag tccggcgacg tgttcgccgg cgagaactgg
     6181 atgacgttcg cgccggtgat cttgtcgatg accgtgaagg acacgcccga cgaggcggcg
     6241 ccgacgatca cgtcgacacc ctcggagagg agccgatccg tcgtctggtt ggcgatgtcg
     6301 gtgttggtgt caccggagtc gcccggcgag tactcgacat ctgcgccgag cacaccacc
//
LOCUS       DASTFV010000509         3398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1881040, whole genome shotgun sequence.
ACCESSION   DASTFV010000509 DASTFV010000000
VERSION     DASTFV010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881040"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..683
                     /locus_tag="VFP02_09335"
     CDS             96..683
                     /locus_tag="VFP02_09335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HET9543273.1"
                     /translation="MRDAPPVDDGRSPRERMEAGDLYRVDDELATDGRRAMARCETYN
                     ALGVDQADERCQLLEDLLGNIGPGTVIRPPFHCDYGRYLSIGARTFANFGLVALDCAL
                     ITIGDDVQIGPNVALLTPTHPIDPDLRRAGWEAAQPITIGDNVWLGGGAIVLPGVTIG
                     SDTVVGAGAVVSRDLPQGVVAVGNPARVIRTVRDG"
     gene            complement(684..1190)
                     /locus_tag="VFP02_09340"
     CDS             complement(684..1190)
                     /locus_tag="VFP02_09340"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9543274.1"
                     /translation="MTTPRAERPAMADYGVPDELDGVLPWSWAEERLLACRNYWVVTA
                     SPSARPHAMPVWGVWVPETGHFAFSCAPGSRKVRNLEANPQVTVAVDDSVEVVVLEGR
                     AAFLDPVTVTDTLHAFATKYWDDPQAVTDGEAFLAQNKVVEVTPERAFGIIEDEVDFS
                     QRATRWVW"
     gene            1267..2745
                     /locus_tag="VFP02_09345"
     CDS             1267..2745
                     /locus_tag="VFP02_09345"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HET9543275.1"
                     /translation="MRGAMTRRQFMARAGITAVAAPGFASLLAACGGSSDSSAAPTSS
                     APVTEAYDPDTPWWLQGNFAPVTQELEAFDLTVAGELPAALTGLYARNGSNARQADNG
                     HWFLGDGMLHGVRLDEGQAAWYRNRWVRTGLYESGAGFTDGPPGGETTYSNVSAFTHA
                     GRLLTSGEIGYPYELSVDDLSTVGAHDFAGRLTTSMTAHPKIDPDTGFMHFFGYGFAE
                     PFLTYHVADERGRLVSSQPVDVAGPTMIHDFAITDQDVVFWEFPVVFDIDLALAGEGM
                     PFRWDASYGARIGVLPLGGPTTAARWVEVDPAYVFHGINAYRSGDDVIVDVSQFATMF
                     DGGVLGQEGAIHRWTIGTGDAALTYRDDVLQSELPMELPTRDPRRVGREHRYGWLVES
                     PVDPDTVRFGGLYRLEVSTGETQYWDAGPGRSANEGLFVPRPGGTDEDDGWVLTYVYD
                     AATEGSELVVLDASAIDGGPLATVELPGRVPHGFHAAWVPAH"
     gene            2776..3054
                     /locus_tag="VFP02_09350"
     CDS             2776..3054
                     /locus_tag="VFP02_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543276.1"
                     /translation="MGKRADRAKAKAEDMADDTVTGLRSGAHTVVDGAEELLGKAKHK
                     TKKARRHVDDIADKAEKKIASMRRKTDRKAKKIRKKAQKQIDKATKKL"
     gene            complement(3069..>3398)
                     /locus_tag="VFP02_09355"
     CDS             complement(3069..>3398)
                     /locus_tag="VFP02_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543277.1"
                     /translation="AWRTGSSYEWGQHVRIGLDSGLAEAEIEALATESADGAWSDADR
                     LAITVTDELCTTDDLTDDTWDAALTHWGEQDLMELIFLVGNYRMLAGFLNAARVELDD
                     GLEGFPS"
BASE COUNT          530 a         1223 c         1160 g          485 t
ORIGIN      
        1 gaacagctcg taggtcccgt cgacgaggtg cacctggagg cccatccccg catcttccca
       61 ggtcgaccgg cccggtccag tgccagagtg tcggcgtgcg tgacgcccca cccgtcgacg
      121 acggccgttc cccgcgggag cgcatggagg ccggcgacct ctaccgggtc gacgacgagc
      181 tggccaccga cggccggcgg gccatggccc ggtgcgagac ctacaacgct ctcggcgtcg
      241 accaggccga cgagcgctgc cagctcctcg aggacctcct cgggaacatc ggccccggca
      301 ccgtcatccg gccgccgttc cactgcgact acgggcggta cctgtccatc ggggcgagga
      361 ccttcgccaa cttcggtctg gtcgccctcg actgcgccct gatcaccatc ggcgacgacg
      421 tccagatcgg gcccaacgtg gcgctgctca cgcccaccca tccgatcgac cccgacctgc
      481 gccgggccgg ctgggaggca gcccagccca tcaccatcgg cgacaacgtc tggctcggcg
      541 gcggcgccat cgtccttccg ggcgtgacca tcgggtccga caccgtggtg ggggcgggcg
      601 ccgtggtcag ccgggacctg ccccagggag tcgtcgccgt cggcaacccg gctcgcgtca
      661 tccgcaccgt ccgcgacggt tgatcaccag acccagcggg tggcccgctg ggagaagtcg
      721 acctcgtcct cgatgatgcc gaaggcccgc tcgggggtga cctcgacgac cttgttctgg
      781 gcgaggaagg cttcgccgtc ggtcactgcc tgggggtcgt cccagtactt cgtggcgaag
      841 gcgtgcaacg tgtcggtcac cgtcaccggg tccaggaacg ccgcccggcc ctccagcacg
      901 accacttcga ccgagtcgtc cacggccacc gtcacctgtg gattggcctc gaggttgcgg
      961 accttccggc tgcccggcgc gcacgagaag gcgaagtgac cggtctcggg gacccacacg
     1021 ccccagaccg gcatggcatg gggccgggcc gagggcgacg ccgtgacgac ccagtagttg
     1081 cggcaggcca agagccgctc ttcggcccag gaccagggca acaccccgtc gagctcgtcg
     1141 gggacgccgt agtcggccat ggcgggacgc tcggcccggg gggtggtcat gtcggttcct
     1201 ctcgttcgcg agccatcgcg gtaccggccc agtgtgaccg cagcggcgtc tacgctcccg
     1261 acccgcatgc gaggggcgat gacacggcga cagttcatgg cccgagccgg catcacggcg
     1321 gtcgccgccc ccggcttcgc ctcgctcctg gccgcttgcg gcggaagtag cgactcctcg
     1381 gctgcgccga ccagctccgc acccgtgacc gaggcctatg acccggacac cccgtggtgg
     1441 ctccagggga acttcgctcc ggtcacccag gagctggagg cgttcgacct caccgtcgcc
     1501 ggcgagctgc ctgccgccct caccgggctc tatgcccgca atggctccaa cgcccgccag
     1561 gccgacaacg gacactggtt cctgggcgac gggatgttgc acggcgtccg tctcgacgag
     1621 ggccaggcgg cctggtaccg gaaccgctgg gtgcgcacgg gcctctacga atcgggggcc
     1681 ggctttaccg acggcccgcc cggaggcgag acgacctaca gcaatgtgtc cgccttcacc
     1741 cacgccggtc gcctcctcac gtccggcgag atcggctacc cctatgagct gtccgtcgac
     1801 gacctgagca ccgtcggggc ccatgacttc gccggccgcc tcacgacctc gatgacggcc
     1861 caccccaaga tcgacccgga caccgggttc atgcacttct tcgggtatgg cttcgccgag
     1921 ccgttcctca cgtaccacgt ggccgacgag cggggccggc tggtctccag ccaacccgtc
     1981 gacgtcgccg gcccgacgat gatccacgac ttcgccatca ccgatcagga cgtcgtgttc
     2041 tgggagttcc cggtcgtctt cgacatcgac ctggccctgg ccggggaggg gatgcccttc
     2101 cgctgggacg cctcctacgg cgcccgcatc ggcgtgctgc cgctgggcgg cccgacgacg
     2161 gcggcccggt gggtcgaggt ggacccggcc tacgtcttcc acgggatcaa cgcctaccgg
     2221 tccggcgatg acgtcatcgt cgacgtcagc cagttcgcca cgatgttcga cggcggcgtg
     2281 ctcggccagg agggcgcaat acaccgctgg acgatcggta ccggcgacgc cgccctcacc
     2341 taccgcgacg acgtgctcca gagcgagctc ccgatggagc tgccgacccg ggacccgcgc
     2401 cgggtgggcc gggaacaccg ctacggctgg ctggtcgagt cccctgtcga ccccgacacc
     2461 gtgcgcttcg gcgggctcta ccgcctggag gtgagcacgg gcgagaccca gtactgggac
     2521 gccgggccgg gccggtcggc caacgagggg ctgttcgtac cccgtcccgg tggcaccgac
     2581 gaagacgacg gctgggtcct gacctacgtc tacgacgccg ccacagaggg aagtgagttg
     2641 gtggttctgg atgcctcggc gatcgatggg ggcccgctgg cgaccgtcga gttgccgggg
     2701 cgggtgcctc acgggttcca tgccgcttgg gtcccggcac actgaacgct gatcaccgtc
     2761 gacgaggagc agaagatggg taagcgagcc gaccgggcaa aggcgaaggc cgaggacatg
     2821 gccgacgaca cggtgacggg gctgcggtcc ggcgcccaca cggtggtcga cggggccgag
     2881 gagctcctcg gcaaggccaa gcacaagacg aagaaggcac ggcgccacgt cgacgacatc
     2941 gccgacaagg ccgagaagaa gatcgccagc atgcgacgca agaccgaccg caaggcgaag
     3001 aagatccgca agaaggccca gaagcagatc gacaaggcca ccaagaagct ctgagccggc
     3061 cttgttgttc agctcgggaa cccctccagg ccgtcgtcga gctcgacccg ggcggcgttg
     3121 aggaagccgg ccagcatccg gtagttgccg acgaggaaga tcagctccat caggtcctgc
     3181 tcgccccagt gggtcagcgc ggcgtcccac gtgtcgtcgg tcaggtcgtc ggtcgtgcag
     3241 agctcgtcgg tgacggtgat cgccagacgg tcggcgtcgg accaggcgcc atcggccgac
     3301 tcggtggcca aggcctcgat ctcggcctcg gcgaggcccg agtcgaggcc gatgcgcacg
     3361 tgctggcccc attcgtagct cgaccccgtg cgccaggc
//
LOCUS       DASTFV010000510         2952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1881289, whole genome shotgun sequence.
ACCESSION   DASTFV010000510 DASTFV010000000
VERSION     DASTFV010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881289"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VFP02_09360"
     CDS             <1..407
                     /locus_tag="VFP02_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HET9543278.1"
                     /translation="TENGNPLYWDEAVAEELAGGPIAPPSMLSVWFRPHHWAPGVSEP
                     QVPLKVHFDLKETLGLPEAVMSDNVITFGEPVRPGDRITTRQILRSVSDEKTTKLGTG
                     RFWVIDVEYHTQDGTWVGTESYTGFGYRRADA"
     gene            404..889
                     /locus_tag="VFP02_09365"
     CDS             404..889
                     /locus_tag="VFP02_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543279.1"
                     /translation="MSLVLGDVSVGDGVPELAYDVTATTVVLGALATRDWRPMHHDHK
                     FATERNGTQDIFLNTPNQAAWFERFLTDWTGPKGRLGQLRFRMLGSVFPEDRMVLRGT
                     VTAVATDEDGCGWVDVDLTLRVGDRTCTTGHARVAVPTDQDDNPWARRGDAWRPFPPE
                     E"
     gene            894..1988
                     /locus_tag="VFP02_09370"
     CDS             894..1988
                     /locus_tag="VFP02_09370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9543280.1"
                     /translation="MDLEFSGEQDMLREMVRGVLAQHSPLTKVRELEDDPQGFDPALW
                     EQLKALDLVGLLLPEAAGGSGQSMLEGVILYEELGRSLAPTPHFVSAVLTGGVLAAAG
                     GQDDLLGAIASGDTVATVAWLEPDGAYRPRGVQTRADGDRLTGVKRHVPYAAGADVLL
                     VLARTGDGEDDVELFLVDREAEGVTLTQQFTIASDTQYRVDLDGAVGTKVEGATWETW
                     HRVMLDGAVLQAASAVGGARYALDITNQYAKDRQQFGKPIGAFQSLSHYLADAVTRVD
                     GAQVLVYEAAWARSMGRPIDQLAPMAKLFADQTFRDVTAMGQQIWGGVGFTLEYDIQL
                     FFRRAKAMQLNWWDARHLEDLVADAVLSGV"
     gene            complement(2013..>2952)
                     /locus_tag="VFP02_09375"
     CDS             complement(2013..>2952)
                     /locus_tag="VFP02_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HET9543281.1"
                     /translation="VLGGGDYPNRRIVNMNRFGAVLAGEGGPVDVLFVQGSNPAASAP
                     NQPAVHEGLAREDLFTVVHDQVMTDTARFADIVLPATTSFEVDDIVAGYGGYWVQDSP
                     AVIPPVGESRPNNQVAHGLAVRLGLESEAFDPSPARLRELMVGPFELPLRLQPEGAVQ
                     FKDLWPAHAGSDSRKARLVTDDPTSDRLPTYRDNDVTGGSLILLSPATNRTITSMFGE
                     YDSSDPSVHLNPVDAESRGLTQGQQAVVTNGQASITVAVAVDDAIRPGVASIPKGLWC
                     KSTPEGLTANAFAPDTLSDLAGGARFNDAMVEVQPA"
BASE COUNT          426 a         1055 c         1054 g          417 t
ORIGIN      
        1 cgaccgagaa cggcaacccc ctctactggg acgaggccgt ggccgaagag ctggcgggcg
       61 ggccgatcgc gccaccgtcg atgctctcgg tctggttccg accccaccac tgggcgcctg
      121 gggtgtccga gccccaggtc cccctgaagg tccacttcga cctgaaggag acgctcggcc
      181 tgcccgaggc cgtgatgagc gacaacgtca tcaccttcgg cgagcccgtc cgccccggcg
      241 accgcatcac cacccgccag atcctgcggt ccgtgtccga cgagaagacc accaagctgg
      301 gcaccggccg cttctgggtg atcgacgtcg agtaccacac ccaggacggg acgtgggtgg
      361 gcaccgagtc ctacaccggc ttcggctacc ggcgggccga cgcatgagcc tcgttctggg
      421 cgacgtgagc gtgggcgacg gggtgcccga gctggcctac gacgtgaccg ccacgaccgt
      481 ggtcctgggg gcactggcca cgcgggactg gcggccgatg caccacgacc acaagttcgc
      541 caccgagcgc aacggcaccc aggacatctt cctgaacacg cccaaccagg cggcctggtt
      601 cgagcgtttc ctcactgact ggaccggccc caaagggcgc ctcgggcagc tccggttccg
      661 gatgctcggc tcggtcttcc ccgaggaccg catggtcctg cggggcacgg tcacggcggt
      721 ggccactgac gaagacggct gcggctgggt cgacgtcgac ctgaccctga gggtcggcga
      781 ccggacgtgc acgacgggcc atgcccgcgt cgccgtcccc accgaccagg acgacaaccc
      841 ctgggcccgc cgcggcgacg cgtggcggcc cttcccacct gaggagtagc gagatggacc
      901 tggagttcag cggcgagcag gacatgctgc gggagatggt gcggggcgtg ctggcccagc
      961 acagcccgct gaccaaggtc cgagagctgg aggacgaccc ccagggcttc gaccccgccc
     1021 tctgggagca gctgaaggcc ctcgacctgg tcggcctgct gctgcccgag gcggccggcg
     1081 gctcgggcca gtcgatgctg gagggcgtca tcctctacga ggagctgggc cggtccctgg
     1141 cccccacgcc gcacttcgtc agcgccgtgc tgacaggtgg cgtgctggcc gcggccggcg
     1201 gtcaggacga cctcttgggc gccatcgcct cgggcgacac ggtcgccacc gtggcctggc
     1261 tggagcccga cggcgcctac cgcccgcggg gcgtccagac ccgggccgac ggcgaccgcc
     1321 tgaccggcgt caagcgccac gtgccctacg ccgccggcgc ggacgtcttg ctcgtcctgg
     1381 cccgcacggg ggacggcgag gacgacgtcg agctgttcct ggtcgaccgg gaggccgagg
     1441 gcgtcacctt gacccagcag ttcaccatcg cctccgacac ccagtaccgg gtcgacctcg
     1501 acggcgccgt gggcaccaag gtcgaggggg cgacctggga gacctggcac cgggtcatgc
     1561 tcgacggggc cgtcctccag gccgcctccg ccgtgggcgg ggcccgctac gcccttgaca
     1621 tcaccaacca gtacgccaag gaccggcagc agttcggcaa gcccatcggc gccttccagt
     1681 cgctgtccca ctacctggcc gacgccgtga cccgggtcga cggcgcccag gtcctcgtct
     1741 acgaggccgc gtgggcccgg tcgatggggc ggccgatcga ccagctcgcc cccatggcca
     1801 agctcttcgc cgaccagacc ttccgcgacg tcaccgccat gggccagcag atctggggcg
     1861 gcgtcggctt caccctcgag tacgacatcc agctcttctt ccgccgggcc aaggccatgc
     1921 agctcaactg gtgggacgcc cgccacctgg aagacctcgt cgccgacgcg gtcctctcgg
     1981 gagtctgacc ccgctcgggt tgcacctgcg cttcaagccg gctggacctc gaccatggcg
     2041 tcgttgaacc gggcgccccc tgcgaggtcg gacagcgtgt cgggggcgaa ggcgttggcg
     2101 gtcaggcctt ccggcgtcga cttgcaccag aggcccttcg ggatcgacgc cacgccgggg
     2161 cggatggcgt cgtctacggc gacggcgacc gtgatcgatg cctggccgtt ggtcacgaca
     2221 gcctgctggc cctgcgtcag gcctctcgac tcggcgtcga ccgggttcag gtgcacgctc
     2281 ggatccgatg agtcgtactc gccgaacatg gacgtgatgg tgcggttcgt ggcgggcgac
     2341 agcaggatca atgagccccc cgtgacgtcg ttgtcccggt aggtcgggag gcggtcggag
     2401 gtcgggtcgt cggtgaccag gcgggccttg cgggagtcgg agccggcgtg ggcgggccac
     2461 aggtccttga actggaccgc accctcgggc tggaggcgca acggcagctc gaacggcccg
     2521 accatcagct cccgcaggcg cgccgggctg gggtcgaagg cctcactctc caagccgagg
     2581 cggacggcca ggccgtgggc cacctggttg ttgggccggc tctcgccgac cggcgggatc
     2641 acggccgggg agtcctgcac ccagtagccg ccgtagccgg cgacgatgtc gtcgacctcg
     2701 aagctggtcg tggccggcag cacgatgtcg gcgaagcggg cggtgtcggt catcacctgg
     2761 tcgtggacca cggtgaacag gtcctcccgg gccaggccct cgtggaccgc gggctggttg
     2821 ggggccgacg cggccgggtt ggagccctgg acgaagagga cgtcgaccgg gccgccctcc
     2881 cccgccagga cggcgccgaa gcggttcatg ttgacgatgc gccggttcgg gtagtcgccg
     2941 ccgccgagta cg
//
LOCUS       DASTFV010000511         2465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1884941, whole genome shotgun sequence.
ACCESSION   DASTFV010000511 DASTFV010000000
VERSION     DASTFV010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884941"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2316)
                     /locus_tag="VFP02_09380"
     CDS             complement(<1..2316)
                     /locus_tag="VFP02_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HET9543282.1"
                     /translation="MAATAGRRGSLRIYLGAAPGVGKTFAMLNEGRRRRERGSDVVVA
                     YAVSHGRAKTAEQVGDLEVVPRRRLVHQGADFEEMDLAAVLARRPEIALVDELAHTNV
                     PGSRHEKRWEDVEALLAEGIDVVSTVNIQHLESLNDVVERITGVRQRETVPDHVVRSA
                     EQVELVDMTPEALRRRMAHGNIYAPEKIDAALGNYFRAGNLGALRELALLWVADRVDD
                     ALEEYRDRHGITETWELRERVLVAVTGAPGTEHLIRRASRIAQRTHGEMIGVHVTADS
                     GLTSAAGALVQAQRRLLEELGGEFREVVASDVSVALIDVARAENATQIVLGASRRSRL
                     QRLTQGSVISRVIARAGPIDVHVISAESTEEERQALPLRRRVLTPVSPARQAWGWGLA
                     VAGLPLMTVALANLRQDVHLPTVLLLYLGLGMTVALVGGAFPALATVVGGFLLADYYF
                     TEPLYDLKIGNTEVVVSLVVYLLAAGLVAVLVDRLGRTRLQAARSHAEAEAMAALAGS
                     LAEAEALPHLLAHLRATFGMRNAALFRGEGAEGPNRRWVVEASVGSPVPDGPEGADVS
                     RDIGPDLVLTLSGRPMQAADEGVLTALAGQLATAVEAQRLQEQAGQASELAAANELRV
                     ALLQAVSHDLRTPLAGIKASISSLRQREITWPPEQEAEFLRTIEEETDRLDNLVENLL
                     DMSRLQSGAVRTQLAPVTVDGVVLTALAGLGPRADDVIVDVDEGLPPVVADRALLERV
                     MANLVDNALKMAPTGTPVRVEAGTVPEGVDIR"
BASE COUNT          331 a          889 c          904 g          341 t
ORIGIN      
        1 gcgaatgtcg actccctccg gcacggtgcc cgcctccacg cgcaccggtg tgccggtggg
       61 cgccatcttg agcgcgttgt cgaccaggtt ggccatcacc cgctcgagca gggcccggtc
      121 ggccacgacc ggcgggaggc cttcgtccac gtccacgatc acgtcgtcgg cccggggccc
      181 gaggccggcc agcgcggtga gcaccacccc gtcgacggtg acgggcgcca gctgcgtgcg
      241 aacggcgccg gactggaggc ggctcatgtc gagcaggttc tccaccaggt tgtccaaccg
      301 gtcggtctcc tcctcgatgg tgcgcaggaa ctccgcctcc tgctccggcg gccaggtgat
      361 ctcccgctgg cggaggctgc tgatcgaggc cttgatgccg gccaacgggg tccggaggtc
      421 gtgggacacg gcttgcagga gcgcgacccg gagctcgttc gccgcggcca gctccgaggc
      481 ctgcccggcc tgctcctgga ggcgctgggc ctcgaccgca gtcgccagct ggccggccag
      541 ggcggtgagg acgccttcgt cggccgcctg catcggccga ccggacaggg tgaggaccag
      601 gtccggcccg atgtcgcgac tgacgtcggc tccctcgggc ccgtccggga cggggctccc
      661 gacggatgcc tcgaccaccc agcggcggtt cgggccttcc gccccttccc cccggaagag
      721 ggcggcgttg cgcatgccga acgtggcccg caggtgggcc aggaggtgcg ggagggcttc
      781 ggcttcggcg agggacccgg ccagcgcggc catggcctca gcctcggcat ggctgcgggc
      841 cgcttggaga cgggttcggc cgagccggtc gacgaggacg gccaccaggc ccgcggccag
      901 caggtagacg acgagggaga ccaccacctc cgtgttaccg atcttcaggt cgtagagcgg
      961 ctcggtgaag tagtagtcgg cgagcaggaa ccccccgacg acggtggcca gcgcggggaa
     1021 cgccccgccc acgagggcca cggtcatgcc caggccgagg tagaggagca gcacggtggg
     1081 caggtggacg tcctggcgca ggttggccag cgccaccgtc atcagcggga ggcccgccac
     1141 cgccaggccc cacccccacg cttggcgggc gggggacacc ggggtcagga cccgacggcg
     1201 gaggggcagg gcctgtcgct cctcctcggt gctctcggcg gagatgacat ggacgtcgat
     1261 gggcccggcc cgggcgatca ctcggctgat gaccgagccc tgggtgaggc gctgcagccg
     1321 gctccggcgg ctggccccga gcacgatctg ggtggcgttc tcggcccggg cgacgtcgat
     1381 cagggcgacg gagacgtcgg aggccacgac ctcccggaac tcgcccccga gctcctccag
     1441 cagccgccgc tgggcctgga ccagtgcgcc cgccgccgac gtcaggccgc tgtcggcggt
     1501 cacgtggacc ccgatcatct cgccgtgcgt gcgctgagcg atgcgggacg cccgccggat
     1561 caggtgctcg gtcccggggg ccccggtgac ggcgactagg acccgctccc gcagctccca
     1621 ggtctcggtg atgccgtggc ggtcgcggta ctcctcgaga gcgtcgtcga cccgatcggc
     1681 cacccacagc agggccagct cccgcagcgc ccccaggttc cccgcccgga agtagttgcc
     1741 cagggcggcg tcgatcttct cgggggcata gatgttgccg tgggccattc gtcggcgcag
     1801 ggcctccggg gtcatgtcca ccagctccac ctgctcggcg gaccgcacga cgtgatcggg
     1861 caccgtctcc cgctggcgaa ccccggtgat gcgctcgacg acgtcgttca gcgactcgag
     1921 gtgctggatg ttcaccgtgg agacaacgtc tatgccctcg gccagcaatg cctcgacgtc
     1981 ctcccaccgc ttctcgtggc gactgcccgg gacgttggtg tgggccagct cgtcgaccaa
     2041 cgcgatctcc ggccgccggg cgaggaccgc cgccaggtcc atctcctcga aatcggcacc
     2101 ctgatggacc agccggcgcc gggggacgac ctcgaggtcg ccgacctgct cggcggtctt
     2161 cgcccggccg tggctcacgg cgtaggccac gaccacgtcg ctgccccgtt cccggcggcg
     2221 ccgaccctcg ttgagcatgg cgaaggtctt gcctacgccg ggcgccgcgc ccaggtagat
     2281 gcgcagggag ccccgtcggc ccgcagttgc ggccatcggt tccacgctac ggggagccgg
     2341 cctcaggcgg ccgcgtcgag ggcgaggttg agccggagga cgttcacgcc cggctcgccg
     2401 aggaagccga gagaccgact gtcggtgtgg gcctcgatga gggcgaggac ggtggccagg
     2461 tccaa
//
LOCUS       DASTFV010000512         2336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1885106, whole genome shotgun sequence.
ACCESSION   DASTFV010000512 DASTFV010000000
VERSION     DASTFV010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885106"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..970
                     /locus_tag="VFP02_09385"
     CDS             140..970
                     /locus_tag="VFP02_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HET9543283.1"
                     /translation="MSTTIWLNGELLAGDRPAISAADHGLLVGDGVFETAKVIDGRAF
                     ALRRHLERLRRSADGLGLELPRSDDELTDGVAAVIEANQPGVGRLRITVTGGPGPLGS
                     TRGAEGPTVIMATGPPGTWPPAAEVVVVPWPRNERSPLAGLKTTSYAENVVALAHAHE
                     QGAGEAILANLAGDLCEGTGSNVFVGLDGRLITPPLSSGCLAGITRDLVLETTDAIEA
                     DVSMADFARADEAFLTSSTRDLQPVTRVDGRALPTCPGPLTHAAVDAFARLVEKTSDP
                     "
     gene            complement(983..>2336)
                     /locus_tag="VFP02_09390"
     CDS             complement(983..>2336)
                     /locus_tag="VFP02_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET9543284.1"
                     /translation="RGWPMGSAEVAGEARRYAERTPGSAALFAAAQRSLPGGDSRSTL
                     YHAPHPLFVDEAAGSRVWDVDGHELVDLTNNHTALVHGNAHPVVLDAVRSQLDRGTCF
                     SGPTALQVEVADRLSSRLPGLERVRFCASGSEATLHAVRAARAFTGRPKIAKAEGAYH
                     GSQDDVFVSTHPSPDQAGPRERPNSVPRAEGLGRSALADTVVFPFNDLDGTAAVLRDV
                     GPELAAVLVEPVMGSAGMIPAEPGYLAGVRELAGEVGALFVVDEVITLRLALGGGQAW
                     AGAEADLSCFGKMLGGGFPLGAFGGRVDVMDAFDPTVEGGPALGHPGSMNAWAVGLAA
                     AGATLDHLTAERIDELGRSGDDLRERLRSVVADRGVAFTVTGVGSLFGLHVTAGPVRT
                     FRDTWAEDRELALAVYLGLINEGVLTDPRGAGCLSTVTTGADLDLAVDAFDRVLGRLT
                     "
BASE COUNT          321 a          876 c          850 g          289 t
ORIGIN      
        1 cgtctgcact tcggcaccgg tggcggcatc acctgggact cggaccctca cgccgaatgg
       61 caggagaccg agctgaaggc ccgccgcctc ctcgccgtcg cctcgggtga gctcgtgggc
      121 tcgccatgat caggggccga tgagcaccac gatctggctg aacggcgagc tgctcgccgg
      181 tgaccgtccg gccatcagcg ccgccgacca cggcctgctc gtgggtgacg gcgtcttcga
      241 gacggccaag gtgatcgatg gacgggcctt cgcccttcgc cgccatctcg agcgcctccg
      301 ccgatcggcc gacggcttgg ggctggagct gccccgttcc gacgacgagc tgaccgacgg
      361 cgtggccgcg gtgatcgagg ccaatcagcc gggcgtcggg cgcctgcgca tcaccgtgac
      421 cggcggaccg ggcccgctcg gctccacccg cggcgccgag ggcccgaccg tcatcatggc
      481 gaccggtccg cccggcacct ggccccccgc cgccgaggtg gtcgtggtgc cgtggccccg
      541 caacgaacgc agcccgctcg ccggcctcaa gacgacctcc tacgcggaga acgtcgtcgc
      601 cctggcccac gcccacgagc agggggcggg cgaggccatc ctcgccaacc tggcggggga
      661 cctctgcgaa ggcaccggct cgaacgtctt cgtgggcctc gatgggcgtc tgatcacccc
      721 tcccctgtcg tcgggttgcc tcgccggcat cacccgggac ctggtcctcg agaccaccga
      781 cgccatcgag gccgacgtgt ccatggccga cttcgcccgg gccgacgagg ccttcctgac
      841 ctcctcgacc cgggacctcc aacccgtgac ccgcgtggac gggcgggcgc tgccgacgtg
      901 cccggggccg ttgacccatg ccgcggtcga cgcgttcgcc cggctggtcg agaagacctc
      961 cgacccgtag ggcccggcga ggtcaggtca ggcggcccaa cacgcggtcg aaggcgtcga
     1021 cggcgaggtc gagatcggcc ccggtggtga ccgtggagag gcagccggcg ccccgggggt
     1081 cggtgaggac gccctcgttg atcagcccga ggtagacggc aagggccagc tcgcggtcct
     1141 cggcccaggt gtcccggaac gtgcgcacgg gaccggcggt gacgtggagg ccgaacagcg
     1201 agccgacgcc ggtgaccgtg aacgcgacgc ctcggtcggc caccacggag cggaggcgtt
     1261 cccggaggtc gtcgccactg cgccccaact cgtcgatgcg ttcggcggtc aggtggtcga
     1321 gcgtcgcgcc cgcggcggcg aggccgacgg cccaggcgtt catgctcccg ggatggccga
     1381 gggccggacc gccttccacc gtggggtcga aggcgtccat gacatccacc cgaccgccga
     1441 aggcgccaag ggggaaaccc ccgcccagca tcttgccgaa gcagctcagg tcggcctcgg
     1501 cgccggccca ggcctggcca ccgcccaggg cgaggcgcag ggtgatcacc tcgtcgacca
     1561 cgaacagggc gccgacctcg cccgccaact cccggacgcc cgccaggtag ccgggctcgg
     1621 ccgggatcat ccccgccgac cccatcaccg gctcgacgag cacggccgcc agttcaggcc
     1681 cgacgtcccg caggacggca gccgtgccgt cgaggtcgtt gaaggggaag accacggtgt
     1741 cggccagggc cgaccgtccg agcccctcag cacgcggcac cgagttcggc cgctcccggg
     1801 gccccgcctg gtcggggctg gggtgggtgc tgacaaagac gtcgtcctgg ctcccgtggt
     1861 aggccccttc cgccttggcg atcttcggcc ggccggtgaa cgcccgggcg gcccgcaccg
     1921 cgtggagggt ggcctcggac ccggaggcgc agaagcggac tcgctcgaga ccgggcagcc
     1981 gactcgacag acggtccgcc acctccacct ggagggccgt cgggccggag aagcacgtcc
     2041 cccggtcgag ctgggaacgc acggcgtcga ggacgacggg gtgggcgttc ccgtggacga
     2101 gcgccgtgtg gttgttcgtg aggtcgacca gctcgtggcc gtcgacgtcc cagacccggc
     2161 tgcccgcggc ctcgtcgacg aagagcggat gcggcgcgtg gtacagcgtg ctccgggagt
     2221 cgccccccgg cagtgagcgc tgggcggcgg cgaagagcgc ggccgagccc ggggtgcgct
     2281 cggcgtagcg ccgggcctcc ccggcgacct cagccgagcc catcggccac ccccgc
//
LOCUS       DASTFV010000513         3156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1886350, whole genome shotgun sequence.
ACCESSION   DASTFV010000513 DASTFV010000000
VERSION     DASTFV010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1886350"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..892)
                     /locus_tag="VFP02_09395"
     CDS             complement(161..892)
                     /locus_tag="VFP02_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9543285.1"
                     /translation="MPEPEFLLEVRGLVAGYVPEVNILNGCDLELREGELVGIIGPNG
                     AGKSTLIKALFGLLPVREGTVTLRGEDVTGKPAHELVRAGIGYVPQRDNVFSRLTIAE
                     NLEMGVYQAPKTYAAQRDRVYGMFPRLAERSDQRAGLLSGGERQMLAMGRALMMEPSV
                     LLLDEPSAGLSPVLQTEVFERADQINEAGVSIVMVEQNARRCLQVCDRGYVLDQGQNA
                     YTDSGDALLHDPKVIELYLGTLAKA"
     gene            complement(885..2039)
                     /locus_tag="VFP02_09400"
     CDS             complement(885..2039)
                     /locus_tag="VFP02_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9543286.1"
                     /translation="MADVLAGILLVLGSVAVGLWATSAINAYSQLPSDWEAAGASRTR
                     WLVLLTLFGFVTAPFYILGPKRRMADALSAARASRPVIEATVLAGVDGRPGVPKPDPI
                     LVVDGVRRRFGGLTAVDVDHLEIQRGLITGLIGPNGAGKTTLFNLLTGFDTPDDGTWQ
                     FNGGDMGGSVAHMLSSRGMVRTFQLTKALSKMTVLDNMMLGGIDQRGEHFASAFLRPA
                     WRDEEDVLRERALVLLDRFGMAHMTNEYAGTLSGGQRKLLEMARALMTEPALVMLDEP
                     MAGVNPALTQSLLGHVKSLREEGMSVIFVEHDMDVVRDISDWVVVMAEGSIIAEGPHD
                     DVTGNAAVIDAYLGTDHGESVTEGPVTHAHQEGHIVEEREEALEEGIPDA"
     gene            complement(2045..3040)
                     /locus_tag="VFP02_09405"
     CDS             complement(2045..3040)
                     /locus_tag="VFP02_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9543287.1"
                     /translation="MDWGDIISNALRAGVSREAAVFAIAAMGLNIQFGFTGLLNFGQV
                     GFMAIGAYGVGISITYYGWNPVVALIVGILCCIGLALVLGLPALRLRADYLAIVTIAA
                     SEIIRTVMRSVSLREFSGGSNGINGFSRGFYAWNPFDPQIRYGFGPFRFFGEQLWLIL
                     IGWVIVLVVTLVTFALMRAPWGRVVRSVREDEDAARALGKNAYGYKVQSLIIGGVMGG
                     LGGMVLAIGTQTVQPDSYSPPITFFLYTALILGGLGRVWTPIVGSIFFWMLLSLTDNF
                     LREAVDNRFLGINHIMDGVQVGQVRFMLVGVGLALLMVFRPQGLFGNKQEMALEV"
BASE COUNT          533 a         1060 c         1107 g          456 t
ORIGIN      
        1 accgaaaccg aggcaccgaa gggggcgtgg ggtcgggctg ccggtcatcg ggggcacgcc
       61 cgtcggcgat ggtccgccac gatcccacgc tccgtggcca aatcggaccg ccgagcccgg
      121 tcgtcccgtt ctggcggaac atcggttgac ccgcccgggg ctaggccttg gccagggtgc
      181 cgaggtagag ctcgatgacc ttggggtcgt ggaggagggc gtcgccggag tcggtgtagg
      241 cgttctggcc ttggtcgagg acgtaacccc ggtcacagac ctgcaggcac cggcgggcgt
      301 tctgctcgac catgacgatc gacacgccgg cctcgttgat ctggtcggcc cgctcgaaca
      361 cctccgtctg caggacgggg ctgagaccgg cggagggctc gtccagcagg aggaccgacg
      421 gctccatcat cagggcccgg cccatggcca gcatctgacg ctcaccaccc gacagcagcc
      481 cggcccgctg gtcggaccgt tcggccagac gggggaacat gccgtaaacc cgatcccgct
      541 gggcggcgta ggtcttgggg gcctggtaga cgcccatctc caggttctcg gcgatggtca
      601 gccgggagaa cacgttgtcc cgctggggga cgtagccgat gcccgcccgg accagctcgt
      661 gggccggctt gcccgtgacg tcctcgcccc gcagggtgac cgtgccctcc cgcaccggga
      721 gcaggccgaa cagggccttg atcagggtgg acttgcctgc cccgttgggg ccgatgatgc
      781 ccaccagctc accctcccgc agctccagat cgcaaccgtt gaggatgttg acctcgggga
      841 cgtacccggc caccaggccg cggacctcca gcaggaactc gggttcaggc atcggggatc
      901 ccctcctcga gggcttcttc ccgttcttcc acgatgtggc cctcctggtg ggcgtgggtg
      961 accgggccct cggtcaccga ctcgccgtgg tcggtgccca gataggcgtc gatgaccgcg
     1021 gcgttgccgg tgacgtcgtc gtgggggccc tcggcgatga tcgatccctc ggccatgacc
     1081 acgacccagt cgctgatgtc ccggacgacg tccatgtcgt gctcgacgaa gatgaccgac
     1141 atgccctcct cccggaggga cttcacgtgg cccagcaggg actgggtgag cgccgggttc
     1201 acgccggcca tgggctcgtc gagcatgacc agggcgggct cggtcatgag cgcccgggcc
     1261 atctccagca acttccgctg gccccccgag agggtcccgg cgtactcgtt ggtcatgtgg
     1321 gccatgccga agcggtcgag caggacgagg gcccgttccc gcagcacatc ttcctcgtcc
     1381 cgccaagctg ggcggaggaa ggcggaggcg aagtgctcgc ctcgctggtc gatgccgccc
     1441 agcatcatgt tgtcgaggac ggtcatcttg gagagggcct tggtcagctg gaaggtccgg
     1501 accatgcccc ggctcgacag catgtgggcc accgagcccc ccatgtcccc cccgttgaac
     1561 tgccaggtcc cgtcgtcggg cgtgtcgaag ccggtcagga ggttgaacag ggtcgtcttg
     1621 ccggcgccgt tgggcccgat gagcccggtg atgagccccc gttggatctc caggtggtcg
     1681 acgtcgaccg cggtgaggcc gccgaaacgg cggcgaacgc cgtccaccac caggatgggg
     1741 tcgggcttgg ggacaccggg acggccgtcg acgccggcca gcacggtggc ctcgatcacc
     1801 gggcgactgg cccgggccgc ggacaacgcg tcggccatcc gtcgcttggg gccgaggatg
     1861 tagaagggcg cggtcacgaa accgaacagg gtgagcagga ccagccagcg ggtgcgcgac
     1921 gctccggcgg cctcccagtc cgacggcagc tgggagtagg cgttgatggc cgacgtggcc
     1981 caaaggccga cggccaccga gcccagcacc aggaggatcc ccgcgagcac gtcagccatg
     2041 acggtcacac ctcaagagcc atctcctgct tgttcccgaa caggccctgc ggccggaaca
     2101 ccatgaggag ggccaggccg acaccgacca gcatgaaccg gacctggccg acctgcacgc
     2161 cgtccatgat gtggttgatc cccaggaagc ggttgtccac cgcttcccgg aggaagttgt
     2221 cggtcagcga caggagcatc cagaagaaga tcgagcccac gatcggggtc cacacccggc
     2281 ccaggccccc gaggatcaac gccgtgtaga ggaagaaggt gatcggcggg gagtaggagt
     2341 cgggctggac cgtctgggtg ccgatggcca ggaccatgcc cccgaggcca cccatgacac
     2401 cgccgatgat gaggctctgc accttgtagc cgtaggcgtt cttgccgagg gcccgagccg
     2461 cgtcctcgtc ctcccggacc gagcgcacga cacgacccca cggggcccgc atgagggcga
     2521 aggtgaccag ggtgacgacc aggacgatga cccagccgat gaggatgagc cacagttgct
     2581 cgccgaagaa ccggaacggc ccgaagccgt accggatctg ggggtcgaac gggttccagg
     2641 cgtagaagcc ccgggagaag ccgttgatgc cgttggagcc cccggagaac tcgcgcaggg
     2701 acaccgagcg catgaccgtg cggatgatct cggacgcggc gatggtcacg atggcgaggt
     2761 agtcggcgcg gaggcgcagg gcgggcaggc cgagcacgag ggccaggcct atgcagcaca
     2821 ggatcccgac gatcaaggcc accaccgggt tccagccgta gtacgtgatg ctgatgccca
     2881 cgccgtaggc cccgatggcc atgaagccga cctggccgaa gttgagcagg ccggtgaagc
     2941 cgaactggat gttcagcccc atggccgcga tcgcgaacac cgccgcctcc cggctgacgc
     3001 cggcccgcag tgcgttcgag atgatgtcgc cccagtccat gccgccccct cagccgatcc
     3061 gctcgcgctg gccgaagatg ccctggggcc ggacgaccag gatcaggatc aggagggtca
     3121 gggcgctcac gttcttcagc tcgggcgaga tccaga
//
LOCUS       DASTFV010000514         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1887636, whole genome shotgun sequence.
ACCESSION   DASTFV010000514 DASTFV010000000
VERSION     DASTFV010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887636"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VFP02_09410"
     CDS             complement(<1..588)
                     /locus_tag="VFP02_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HET9543288.1"
                     /translation="MVEVANEPAVVLICVVLASALLVAEFALPTLGLAGALGFGLLFF
                     AIVGINDADLDWWPLSMVAVAVVFWCVMVARRIAPPVQQGVTIALFTAGSVGFGVLAG
                     DVTTVVVALLGSAGLAAGFPVLFERSVRLLGAPSSTGMESFVGLTVPVTAWEGTAGTV
                     VLEGSFWNAVGPPGLTDGDEVVVSGYEGMTLTVRRA"
     gene            731..2053
                     /locus_tag="VFP02_09415"
     CDS             731..2053
                     /locus_tag="VFP02_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9543289.1"
                     /translation="MEGDRITVFLADDNTLVREGVKALLSIEKDIEVVGTAEDYDGLV
                     EGATAAHPQVVVSDIRMPPNFQDEGIAGAKEVRKRHPGTGIVILSQYDDPDYAISLLS
                     EGAAGYAYLLKDRVSDGDRLARAVREVATGGSMLDPEIVQALVNPVRVEGELTEDEDD
                     LLTQVAQGRPIKAIAAGRDRTPAAINDAVEAVLLKLAQGASAGREGALKRLRMLQSAI
                     VDREEQGETLSRFLPGGLAEKLRTDSGAVDRTERLTVTVLMSDVRGYSTIAEHADPTS
                     LAGQLRTHRREMNNAILGEEGTVMQYVGDAVMAVFGAPFPQEDHADRALRAAQAMHHR
                     QSVVNVTWADEGLAAFGLGIGLSSGDVAAALLGSDERLEYTVVGDTVNLAQRLESIAR
                     PAGTTVLSEATVEGLSDPPPLEQMPPQTVKGRVAEVTAYRLAPEEDAD"
BASE COUNT          360 a          755 c          739 g          232 t
ORIGIN      
        1 cgctcggcgc accgtcagcg tcatgccttc gtagccgctg acgacgacct cgtcaccatc
       61 ggtcagcccc gggggcccaa cggcattcca gaacgagccc tcgaggacca cggttccggc
      121 cgtgccctcc caggcggtga cgggcacggt caggcccaca aaggactcca tgccggtcga
      181 cgaaggggcg ccgagcagcc ggaccgaccg ctcgaagagg acggggaagc ccgccgccag
      241 cccggcggag ccgagcaggg ccaccacgac ggtcgtgacg tcgccggcca ggacgccgaa
      301 gccgacggag cctgcggtga agagggcgat ggtgacgccc tgctggacgg gtggggcgat
      361 gcgtcgggcg accatcacgc accagaacac gacggccacg gcgaccatcg agagcggcca
      421 ccagtcgagg tcggcgtcgt tgatgcccac gatggcgaag aagaggaggc cgaagcccag
      481 cgcgccggcg aggccgagcg tggggagcgc gaactcggcc accaggaggg cggacgccag
      541 caccacgcag atcaggacga ccgcgggctc gttggcgacc tcgaccacgg cccaacccta
      601 aacccgatcc ctccccgccc cctctggcgg ggaagggggc gggccgggaa cgccggggtg
      661 ctggcacccc actgaccggt cgggtcctcc cccttgcggg gaccaccgat ccgcggctca
      721 atggcccccg atggaagggg accgaatcac cgtgttcctc gccgacgaca acaccctggt
      781 acgggagggg gtcaaggccc tcctctcgat cgagaaggac atcgaggtcg tgggcacggc
      841 ggaggactac gacggcctgg tcgaaggcgc caccgcggcg cacccccagg tggtggtgtc
      901 cgacatccgc atgcccccga acttccagga cgagggcatc gccggcgcca aggaggtgcg
      961 caagcgccac cccgggaccg gcatcgtcat cctctcccag tacgacgacc ccgactacgc
     1021 catctctctc ctgagcgagg gtgcggccgg gtacgcctac ctgctcaagg accgggtgag
     1081 cgacggcgac cggctggccc gcgccgtccg tgaggtggcc accggtggct ccatgctcga
     1141 ccccgagatc gtccaggccc tggtcaaccc ggtccgcgtg gaaggcgagt tgaccgagga
     1201 cgaggacgac ctcctgaccc aggtggcgca gggccgcccc atcaaggcca tcgccgccgg
     1261 tcgggaccgg accccggcgg cgatcaacga cgccgtcgag gccgtcctcc tcaagctggc
     1321 ccaaggggcc agcgccggcc gggagggtgc gctcaagcgg ctccggatgc tgcagtcggc
     1381 catcgtcgac cgggaggagc agggcgagac cctcagccgc ttcctgcccg gcggcctggc
     1441 cgagaagctg cgcaccgaca gcggcgccgt cgaccgcacc gagcgactca cggtcacggt
     1501 cctcatgtcc gacgtccggg gctactcgac catcgcggag cacgccgacc ccacgtcgct
     1561 ggccggccaa ctccgcaccc accggcgaga gatgaacaac gccatcctcg gtgaggaggg
     1621 caccgtgatg cagtacgtgg gcgacgccgt gatggcggtc ttcggggccc cgttccccca
     1681 ggaggaccac gccgaccggg ccctgcgggc ggcccaggcc atgcaccacc gccagagcgt
     1741 ggtcaacgtg acctgggccg acgagggcct ggccgccttc ggcctcggca tcggcctgtc
     1801 gtcgggcgac gtggccgccg cccttttggg tagcgacgag cggctggagt acaccgtcgt
     1861 cggcgacacg gtgaacctgg cccagcgcct ggagagcatc gcccggcccg caggcacgac
     1921 cgtgctcagc gaggcgaccg tggaaggcct gagcgacccg cccccgctcg agcagatgcc
     1981 gccccagacg gtgaagggcc gcgtggccga agtgacggcg taccggctcg cgccggagga
     2041 ggatgcagac tgatgtccac aggaggaaac acgatgaccg atgacg
//
LOCUS       DASTFV010000515         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1900978, whole genome shotgun sequence.
ACCESSION   DASTFV010000515 DASTFV010000000
VERSION     DASTFV010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1900978"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFP02_09420"
     CDS             <1..292
                     /locus_tag="VFP02_09420"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HET9543290.1"
                     /translation="AKVFCFLFIFVWFRATLPRFRYDQLMDLGWKALIPLALGWLLLL
                     AGIQIARDEDWNWLVFFPVALAVFLAAYALLGRAVRTSRDERAASDGVEVFD"
     gene            292..900
                     /gene="nuoI"
                     /locus_tag="VFP02_09425"
     CDS             292..900
                     /gene="nuoI"
                     /locus_tag="VFP02_09425"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HET9543291.1"
                     /translation="MADFFDYIAGFGVTFKKLFEDRVTAPYPEEKAPKPIRLHGRHVL
                     NRYEDGMEKCIGCELCAGVCPARCIYVRGADNPPDDPISPGERWGFIYEINYLRCIHC
                     DLCVEACPTEAITETKLFEFSFTDRQDAIYTRDELLVDDEGKPQQLPWEDWREGEDLF
                     SSAWMRATASAGNAEYEGVVAWSGELGYGVRAPEQGQRAEDD"
     gene            900..1469
                     /locus_tag="VFP02_09430"
     CDS             900..1469
                     /locus_tag="VFP02_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET9543292.1"
                     /translation="METVVFIISAAIVLTGAVGVIAFRNPVHNALSLVGTLFGVAVLF
                     VAQEAHLLAAVQVIVYAGAIVILFLFVIMLLGVDQADSMREDPLPGQRPLAIVAGLAF
                     VGLLLVVLVFSTDDPVTGGQALLAGFGDRANVEALARVLFSQYVFAFEITALLLTIAV
                     VGAVVLAKRPAAGSAIEAEDEAARTGEEA"
     gene            1478..1783
                     /gene="nuoK"
                     /locus_tag="VFP02_09435"
     CDS             1478..1783
                     /gene="nuoK"
                     /locus_tag="VFP02_09435"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET9543293.1"
                     /translation="MQVTTTWYLVLGAMLFSLGGTGLLVRRNPLVMFMCVELMLNAVN
                     LTFVAFGRQLGDLGGQVSVFFVLVVAAAEVVVGLALIVAVLRRRPGATADDVAVLKG"
     gene            1788..>2178
                     /locus_tag="VFP02_09440"
     CDS             1788..>2178
                     /locus_tag="VFP02_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET9543294.1"
                     /translation="MIELVWLIPALPLAGFLLLTLAGRRVGEPAAGWLATLLMGASFA
                     AAVAVHLGLVDRGVEERAFSQVLFEWLPVGGLHVDLGFLVDPLSMTMTLFITGVGTLI
                     HLYSVGYMHGDRDFARFFALLNLFVFFM"
BASE COUNT          267 a          787 c          722 g          402 t
ORIGIN      
        1 cgccaaggtc ttctgcttcc tcttcatctt cgtctggttc cgggccaccc tgccccgctt
       61 ccgctacgac cagctgatgg acctgggctg gaaggccctc atcccgttgg ccctgggctg
      121 gctcctgttg ctggccggca tccagatcgc ccgggacgag gactggaact ggctcgtgtt
      181 cttcccggtc gcgctggccg tcttcctggc ggcctacgcc ctgctcggac gggcggtccg
      241 cacctcacgg gacgagcggg ccgcatcgga cggggtggag gtgttcgact gatggccgac
      301 ttcttcgact acatcgccgg cttcggggtc acgttcaaga agctgttcga ggaccgggtc
      361 accgcgccct atcccgagga gaaggctccc aagccgatcc gcctccacgg ccgtcacgtc
      421 ctgaaccggt acgaggacgg catggagaag tgcatcggct gcgagctgtg tgccggggtc
      481 tgtccggccc gctgcatcta cgtgcggggg gccgacaacc cgccggacga cccgatcagc
      541 cccggcgagc gctggggctt catctacgag atcaactacc tgcgctgcat ccactgcgac
      601 ctctgcgtcg aggcctgccc caccgaggcc atcaccgaga ccaagctctt cgagttctcc
      661 ttcaccgatc gccaggacgc catctacacg cgcgacgaac tgctggtgga cgacgagggc
      721 aagccccagc agctgccctg ggaggactgg cgggagggcg aggacctgtt cagctcggcg
      781 tggatgcggg ccaccgcgtc ggccggcaac gccgagtacg agggtgtcgt ggcctggtcc
      841 ggtgagctcg gctacggcgt gcgcgccccg gagcagggcc agcgggccga ggacgactga
      901 tggaaacggt cgtcttcatc atcagcgcgg ccatcgtgct caccggggcc gtaggcgtca
      961 tcgccttccg caacccggtc cacaacgccc tgtcgctggt ggggaccctg ttcggcgtgg
     1021 ccgtgctctt cgtggcccag gaggcccacc tgctcgccgc ggtgcaggtc atcgtctacg
     1081 ccggtgccat cgtcatcctg ttcctgttcg tcatcatgct cctcggcgtg gaccaggccg
     1141 actcgatgcg ggaggacccc ctgcccggcc agcgcccgct ggcgatcgtg gccgggctgg
     1201 ccttcgtggg cctgctcctc gtggtcctgg tcttcagcac cgacgacccg gtgaccggcg
     1261 gccaggccct cctcgccggt ttcggggacc gggccaacgt ggaggccctg gcccgggtcc
     1321 tcttcagcca gtacgtcttc gccttcgaga tcaccgccct gctgctgacc atcgccgtgg
     1381 tcggcgcggt ggtcctggcc aagcgccccg ccgccggcag cgccatcgag gccgaggacg
     1441 aggccgcccg caccggggag gaggcgtgat cacggcgatg caggtcacca ccacctggta
     1501 cctggtgttg ggggccatgc tcttcagcct cgggggcacg ggcctgctcg tgcgccgcaa
     1561 cccgctggtc atgttcatgt gcgtcgagct catgctcaac gccgtcaacc tgaccttcgt
     1621 ggccttcggc cgccagttgg gtgacctcgg cgggcaggtc tcggtcttct tcgtcctggt
     1681 ggtcgccgcc gccgaagtgg tggtcggctt ggccctcatc gtggccgtcc tgcgccgccg
     1741 cccaggcgcc acggccgacg acgtcgcggt gctgaagggc tgatcgggtg atcgaactcg
     1801 tctggttgat ccccgccctg cccttggccg gcttcctgct cctgaccctc gccgggcgcc
     1861 gcgtcggcga gcccgccgcg gggtggctgg ccaccctgct catgggcgcc tcgttcgcgg
     1921 ccgccgtcgc cgtgcacctc ggcctggtcg accggggcgt cgaggagcgg gccttcagcc
     1981 aggtcctctt cgagtggctg ccggtcggcg gcctgcacgt ggacctcggt ttcctggtcg
     2041 accccctgtc gatgacgatg accctgttca tcacgggcgt cggcaccctg atccacctct
     2101 actcggtcgg ctacatgcac ggcgaccgcg acttcgcccg cttcttcgcc ctgctcaacc
     2161 tcttcgtctt cttcatgc
//
LOCUS       DASTFV010000516         3285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1903456, whole genome shotgun sequence.
ACCESSION   DASTFV010000516 DASTFV010000000
VERSION     DASTFV010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903456"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..395
                     /locus_tag="VFP02_09445"
     CDS             48..395
                     /locus_tag="VFP02_09445"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HET9543295.1"
                     /translation="MSSTDALTDEEYEALPTEPDQTGAHHPTELLYVKVAALLAVITL
                     AEISTYWFDFGDFLLPALITMMVVKFALVIGYFMHLRFDNRIFTRVFLSGLFIAVAVY
                     IVMLTTFLYWQNP"
     gene            402..1328
                     /locus_tag="VFP02_09450"
     CDS             402..1328
                     /locus_tag="VFP02_09450"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET9543296.1"
                     /translation="MLAANEAAWRWQPHPEVWLLVLGVVALSLYVTRVIGPKVVPAGQ
                     PVLSRRQAVCLVAGILTLWLASDWPLHDLGEEYLFSLHMTQHLLLSYVMPLLFLLATP
                     PWLARLVVPEGSASYRWLHRFSRPLVAGLLFNAVVVFTHWPALVNKVVTNGPMHYGIH
                     VLVVASAVLMWMPVFGPFAEWRMSSPPAMAYLFAMSLLPTVPASWLIFADGSVYNVYD
                     TPFRLWGVSVTSDQQAAGVVMKLVGGSYLWMLIGVVFFRWAYAQQSNDPVRIAHTRTP
                     RWDPSDDALTFDAVQAAFDAAGSAPAELDSSA"
     gene            1325..2110
                     /locus_tag="VFP02_09455"
     CDS             1325..2110
                     /locus_tag="VFP02_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9543297.1"
                     /translation="MILEGKTVIVSGVGPGLGGEVARLAVRDGANVVVAARSAEKLAA
                     AATELDPSGDRVLAVPTDIGDLAACTALVTAATDRFGAVHAVAQVAAVDTALGNLAAS
                     SDDDWRRCFDTNVVGSMNVVRAVTPALRDAGGGAVVLIGSQSAVLPPTLMPQIAYAAS
                     KGALLSAMYHLADELGPDRIRVNVVIPTWMWGPPVQMYVDWQSGERQVSPEAVMGEIT
                     ANMSIPEIPADEDVAEAVIWLCSDRARMVTGQSLMVNAGELKG"
     gene            2156..2653
                     /locus_tag="VFP02_09460"
     CDS             2156..2653
                     /locus_tag="VFP02_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543298.1"
                     /translation="MLESAPPDAPRVTASDPDGARTLSGRPVVDGVATRPTTRVGRGT
                     VVFAVTLAVALVVGVSLVLLRRGPEGTDVRAMAPGDCYVETDVVEEDGRSVPYGTDAP
                     CLSISPRIVAVVPLPIGPYPGEAGFDQVVADRCGGEADIIVAPTSNTWPAGDRTVVCI
                     ALPEV"
     gene            complement(2663..>3285)
                     /locus_tag="VFP02_09465"
     CDS             complement(2663..>3285)
                     /locus_tag="VFP02_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9543299.1"
                     /translation="DEVIWTDPPEREEAGSPNVIGAVALHAAMDELTAIGWDAVAAHD
                     DEVAERLRTDLAAVPGVHLLGPDLATATLPTATFTVDGLSHALVAARLSAEFGIGVRH
                     GCFCAHPYLLRLLGLDADEVAAYRGAVLRGDKREIPGAVRASAGLSTSVDDVDRLVAA
                     VTRIAAGEPAPVAYDQDPHTGDFWPQGDRPGWTAADRSFGASCARG"
BASE COUNT          413 a         1212 c         1135 g          525 t
ORIGIN      
        1 ctggatcgtc atcttcaccg tcgtctacct ggcagcctga ggtcacgatg agcagcaccg
       61 acgccctgac cgacgaggaa tacgaggcgc tgccgaccga gccggaccag accggggccc
      121 accaccccac cgagctgctg tacgtgaagg tcgccgcgct cctcgccgtc atcaccctgg
      181 ccgagatcag cacctactgg ttcgacttcg gcgacttcct cctcccggcc ctgatcacga
      241 tgatggtcgt gaagttcgcg ctggtcatcg ggtacttcat gcacctgcgc ttcgacaacc
      301 ggatcttcac ccgggtgttc ctgtccgggc tgttcatcgc tgttgcggtg tacatcgtca
      361 tgctcaccac cttcctctac tggcagaacc cgtaggtccc cgtgctggcc gccaacgaag
      421 ccgcctggcg ctggcagccg caccccgagg tctggctcct ggtcctcggg gtcgtcgccc
      481 tctcgctcta cgtgacccgg gtcatcggcc ccaaggtcgt gcccgccggc cagccggtcc
      541 tgagtcgccg gcaggcggtc tgcctggtcg ccgggatcct gaccctgtgg ctggcctcgg
      601 actggcccct gcacgacctc ggcgaggagt acctgttctc gctgcacatg acccagcacc
      661 tgttgctgag ctacgtgatg ccgctgctct tcctgctggc cacgccgccc tggctggccc
      721 gcctggtcgt ccccgagggg tcggcttcct accggtggct gcaccggttc tcccggcccc
      781 tggtcgccgg cctcctgttc aacgccgtgg tcgtcttcac ccactggccc gcgctggtca
      841 acaaggtcgt gaccaacggt cccatgcact acgggatcca cgtgctggtg gtggccagcg
      901 ccgtgctcat gtggatgccg gtgttcggcc ccttcgccga gtggcgcatg tccagcccgc
      961 ccgccatggc ctacctcttc gccatgtccc tgctgcccac ggtgccggcc tcgtggctga
     1021 tcttcgccga cgggtcggtc tacaacgtgt acgacacgcc gttccgcctc tggggcgtgt
     1081 cggtcacgtc cgaccagcag gccgccggcg tggtcatgaa gctggtcggt ggctcctatc
     1141 tctggatgtt gatcggtgtc gtgttcttcc ggtgggccta cgcccagcag agcaacgacc
     1201 cggtccgcat cgcccacacc cggaccccgc gctgggaccc gtccgacgac gcgctcacct
     1261 tcgacgccgt ccaggccgcc ttcgacgccg cgggctcggc cccggccgaa ctggactcgt
     1321 ccgcatgatc ctcgaaggca agaccgtcat cgtctcgggc gtgggcccgg gcctcggcgg
     1381 ggaggtggcc cggctcgccg tgcgcgacgg ggccaacgtc gtcgtggccg cccggtcggc
     1441 ggagaagctg gcggcggcgg cgaccgagct ggacccctcg ggggaccgcg tcctggcggt
     1501 acccaccgac atcggggacc tggccgcctg cacggccctc gtcaccgccg ccaccgaccg
     1561 cttcggcgcc gtgcacgccg tggcccaggt ggccgcggtc gacaccgccc tcggcaacct
     1621 ggcggcctcg tccgacgacg actggcgccg ctgcttcgac accaacgtgg tcgggtcgat
     1681 gaacgtggtc cgggccgtga cgcccgccct gcgggacgcg gggggtgggg ccgtggtcct
     1741 gatcggctcg cagtccgcgg tcctgccgcc gacgctcatg ccccagatcg cctacgccgc
     1801 ctccaagggc gcgctgctct cggccatgta ccacctcgcc gacgagttgg gccccgaccg
     1861 catccgggtg aacgtcgtca tccccacgtg gatgtggggc ccgccggtcc agatgtacgt
     1921 ggactggcag tccggtgagc gccaggtgtc gcccgaggcg gtcatggggg agatcacggc
     1981 caacatgtcg atccccgaga tcccggccga cgaggacgtg gccgaagcgg tgatctggct
     2041 ctgctcggac cgggctcgca tggtcacggg ccagtccctc atggtgaacg cgggcgagct
     2101 caaaggttga cccggaggcc tcaagaccag aggccgcaca gccgacgggt gaaccgtgct
     2161 cgagtctgct ccccctgatg ccccgcgggt caccgcatcc gacccggacg gggcgcgcac
     2221 gttgtcgggc cgtcccgtgg tcgacggggt ggccacccgg ccgaccacgc gggtcggtcg
     2281 cggaacggtg gtgttcgcgg tgaccctggc cgtggccctg gtcgtcgggg tcagcctggt
     2341 gttgctgcgc cgaggccccg agggcaccga cgtccgggcg atggcgccgg gcgactgcta
     2401 cgtcgagacc gacgtggtgg aggaggacgg tcggtcggtg ccctacggga ccgatgcccc
     2461 ctgcctctcc atctcccccc ggatcgtcgc cgtggtccca ctacccatcg ggccctatcc
     2521 cggcgaggcc ggcttcgacc aggtggtggc cgatcgctgc gggggggagg ccgacatcat
     2581 cgtggccccg acgtcgaaca cctggcccgc gggcgatcgc accgtggtct gcatcgccct
     2641 ccccgaggtc tgatccgggg gctcagcccc gggcgcagga cgcgccgaag ctgcggtccg
     2701 ccgccgtcca gccgggccgg tcgccttggg gccagaagtc gccggtgtgg gggtcctggt
     2761 cgtaggccac cggggccggt tcgcccgccg cgatgcgggt gacggcggcc acgaggcggt
     2821 cgacgtcatc gaccgaggtg gacaacccgg cgctggcccg gaccgcgccg gggatctccc
     2881 gcttgtcgcc ccggagcacc gcaccccggt acgcggcgac ctcgtcggcg tcgagtccga
     2941 gcaggcggag caggtagggg tgggcgcaga agcaaccgtg gcggaccccg atgccgaact
     3001 ccgcgctcag gcgggcggcg accagggcgt gggacagccc gtcgacggtg aaggtggccg
     3061 tgggcagcgt cgccgtggcc aggtccggac cgagcaggtg gaccccgggc acagcggcca
     3121 ggtcggtgcg caaccgttcg gccacctcgt cgtcgtgggc cgccaccgcg tcccagccga
     3181 tggcggtcag ctcgtccatg gccgcgtgca gcgccacggc gccgatcacg ttcggcgagc
     3241 ccgcctcctc ccgctcgggg ggatcggtcc agatgacctc gtcca
//
LOCUS       DASTFV010000517         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1903695, whole genome shotgun sequence.
ACCESSION   DASTFV010000517 DASTFV010000000
VERSION     DASTFV010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903695"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VFP02_09470"
     CDS             <1..1275
                     /locus_tag="VFP02_09470"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9543300.1"
                     /translation="LDREQARVTELESGLAELEDDEDAAKAAGRELRLLRGAIEERAA
                     DLSARRTDLEVRQASLRERQRVAEQRLGEIEPRLAKDVHEREHAESRRSEIDARATAV
                     ERLTTEVEARSADIDQVLDGLRERRRQQSEAVRGVAARLDGLRRERGEAERTLAEVRE
                     RSQREEVREAEVAVRLETAVETLRVELDCEPDRAVATDCPDLPEGNTATGRARELERD
                     LRLMGPINPLALEEFEQLQERHGFLQGQLEDVQESRRELRKVIKAVDDEIRDVFTAAY
                     ADVAANFESLFATLFPGGTGRLSLTDPDNLLDTGLEIEARPSGKNVRKLSLLSGGERS
                     LTALAFLFAVFRSRPSPFYVMDEVEAALDDVNLHRFLDLLAEFRLEAQLIVVSHQKRT
                     MEAGDVLYGVTMQPGGSSRVISERVTESELVN"
     gene            complement(1339..>2135)
                     /locus_tag="VFP02_09475"
     CDS             complement(1339..>2135)
                     /locus_tag="VFP02_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9543301.1"
                     /translation="LLLVSLSLLVVALARPTRPDDAPLGPVLVLAIDTSLSMQATDVD
                     PNRLEAAQEAADGLVEGLSDNVRVGLVSFDNGATIQVTPTTDHDAVREAIDGLSLDEF
                     TAIGEAIFAGLDAIAIAEQDQPEAPEDPAADEPPPSQIVLMSDGETTTGRPDVEAAQA
                     AFDAGVPVSTIAFGTQGATIDIPGQGPVDVSVNEAALQAIADSTSGEYFSATTGDELA
                     DAFADIGGTVDSETVEREVGTWFLGFGLALLVLTSALSLLWFSRLP"
BASE COUNT          315 a          716 c          814 g          290 t
ORIGIN      
        1 ctggaccgcg agcaggctcg ggtgaccgag ctggagtcag gcctggccga gctggaggac
       61 gacgaggacg cggccaaggc cgcggggcgg gagctgcgcc tgctccgggg agcgatcgag
      121 gagcgggcgg cggacctctc cgcccggcgg accgacctcg aggtccggca ggcctcgctg
      181 cgggagcgac agcgggtggc cgagcagcgg ctgggggaga tcgagccccg gctggccaag
      241 gacgtccacg agcgggagca cgccgagagt cgtcggtccg agatcgatgc ccgggccacc
      301 gcggtggaac ggctgaccac cgaggtcgag gcccgatcgg ccgacatcga ccaggtcctc
      361 gacggcctgc gggaacgccg gcgccaacag tcggaggccg tgcggggcgt cgccgcccgt
      421 ctcgacggcc tgcgccgcga acggggcgag gccgaacgca ccctcgccga ggtccgcgaa
      481 cgctcccagc gggaggaggt gcgggaggct gaggtcgcgg ttcgcctcga gaccgcggtc
      541 gagacgctcc gcgtcgagtt ggactgcgag cccgatcggg ccgtggccac cgactgcccg
      601 gacctccctg aagggaacac ggcaacgggt cgggcccggg agctggagcg ggacctgcgc
      661 ctcatggggc cgatcaaccc gctggccctc gaggagttcg agcagctcca ggaacgccac
      721 gggttcctcc agggccagct ggaggacgtc caggagtcgc gccgggagct gcgcaaggtc
      781 atcaaggccg tcgacgacga gatccgcgat gtgttcaccg cggcgtacgc cgacgtggcc
      841 gccaacttcg agtccctctt cgccaccctg ttcccgggtg gcaccggtcg gttgagcctc
      901 accgacccgg acaacctcct cgacacaggc ctggagatcg aggcccggcc ctcgggcaag
      961 aacgtgcgca agctctcgct cctctccggc ggcgagcggt ccctgaccgc gttggccttc
     1021 ctgttcgccg tcttccgcag ccggccgtcg cccttctacg tgatggacga ggtcgaggcg
     1081 gctctcgacg acgtgaacct ccaccggttc ctggatctgc tggccgagtt ccgcctggag
     1141 gcccagctca tcgtggtcag ccaccagaag cggaccatgg aggccggcga cgtcctctac
     1201 ggcgtgacca tgcagcccgg tggttcgtcc agggtcatct ccgagcgggt cactgagtcc
     1261 gagctcgtga actagagcgc accggccctg ccagcaggga ggcggcgcag tccgagtggg
     1321 gcgaagccac tcgaggggtc acggcagtcg gctgaaccag aggagtgaca gggccgaggt
     1381 cagtaccagg agggcgaggc cgaagcccag gaaccaggtg ccgacctccc gctcgacggt
     1441 ctcggagtcg accgtgccgc cgatgtcggc gaaggcgtcg gccagctcgt cgccggtcgt
     1501 cgccgagaag tactcgccgc tggtcgagtc ggcgatggcc tggagggccg cctcgttgac
     1561 cgacacgtcc accggcccct ggcccgggat gtcgatggtc gctccctgcg tgccgaaggc
     1621 gatggtcgac acgggcacgc ccgcgtcgaa ggcggcctgg gcggcctcca catcggggcg
     1681 gccggtcgtg gtctcgccgt cggacatgag cacgatctgg ctgggtggcg gctcgtcggc
     1741 cgccggatcc tcgggcgcct cgggttggtc ctgctcggcg atggcgatgg cgtcgaggcc
     1801 ggcgaagatg gcctccccga tggcggtgaa ctcgtccagg ctgaggccgt ctatggcctc
     1861 gcggacggcg tcgtgatcgg tggtcggtgt gacctggatg gtggccccgt tgtcgaagct
     1921 gacgaggccg actcggacgt tgtcggagag gccttcgacg aggccgtcgg ccgcctcctg
     1981 ggccgcctcc aggcggttgg ggtcgacgtc ggtggcctgc atggacagcg aggtgtcgat
     2041 ggccaggacg aggacgggac ccagcggcgc gtcgtcgggc cgggtcggtc gggccagcgc
     2101 caccaccagg agcgacagcg agacgagcag caggc
//
LOCUS       DASTFV010000518         3802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1910889, whole genome shotgun sequence.
ACCESSION   DASTFV010000518 DASTFV010000000
VERSION     DASTFV010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910889"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2026
                     /locus_tag="VFP02_09480"
     CDS             <1..2026
                     /locus_tag="VFP02_09480"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HET9543302.1"
                     /translation="IRDRDLLLNGERVLIRGVNRHDHHPDRGKTLTREDMERDVVLMK
                     RFGFNAVRCAHYPNDPTFLDLCDEYGLWVIDEANVESHARQLALAHDPRYHAAIVERV
                     VRMVRRDRNHPSVIAWSLGNESGYGIAHDAAAAWVRRTDPSRFLHYEGAIMHSWLTEP
                     GVGAAVTDVVCPMYPQIADIVAWAERGDDRRPMILCEYQHAMGNSNGSLADYWAAFES
                     TPGLQGGFIWDWIDQGLTWLDALGRPYFAYGGAFGDEPNDADFCGNGMVGSDRVPHPA
                     CFEHAKLAQPVEVEALNLHEGRFRLRNRRTFTGLNDVDGHWTLTVDGEAVAEGRVLVP
                     AVPPLGTAEIDVPIERPTSLGQGQRCHLLLSFVLGEGQAWAPRGFEVAWSQFEIPWVA
                     AQPAPIARARRVRIAVDVDEGTVSAGDLLVTSPTACIWRAPTQNDGLRVGPMAERAPG
                     VRRRWLRWGLDRLINELVSSVPRDEGINPSLTVHRRLSGVDPDVRIDHRQVAMLVGGT
                     LVFDEEIRIPEVLDDLPRVGITFTAAAGLEHLTWAGLGPHESYPDRRVGARFGRWTST
                     VTDQYVPYLLPQEHGAHADTRRFTLTDDEGHGLAVSAQEPFSFSVSHFSADDLHSAAA
                     TADLQPRDEVMVHVDVAQRGLGTAACGPDTLPEYRVAGGTFRWRWTLTPL"
     gene            complement(2036..2452)
                     /locus_tag="VFP02_09485"
     CDS             complement(2036..2452)
                     /locus_tag="VFP02_09485"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9543303.1"
                     /translation="MTFRLSTDPADVDVDVVATFLHDEAYWSLGRPRAVVERSLAASR
                     LYTLVAEDGTMAAFARVVTDGVTFGWICDLFVVAAHRGQGLGHQLIEAIVADPDLAGM
                     KRLLLATADAHGVYADVGFVPLLDPTLWMERPGPGA"
     gene            complement(2449..3042)
                     /locus_tag="VFP02_09490"
     CDS             complement(2449..3042)
                     /locus_tag="VFP02_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9543304.1"
                     /translation="MSTTPRLPAARRRRQLLDTARTVFAERGFHATAMNDIADAAGVT
                     KPVLYQHFRSKRDLYREVLEDVGGRLEETIAKAAAGAPGPREQVEAGFGAYFRFVAGD
                     QDAFQVLFGGDTRRDPEFARQAAATEASIATAIAGLIDIEGIAPAHQALLAHGLVGLA
                     EGASRRWLVDGLREDPAVLAAQVAELAWAGLRGVRGP"
     gene            complement(3045..3296)
                     /locus_tag="VFP02_09495"
     CDS             complement(3045..3296)
                     /locus_tag="VFP02_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543305.1"
                     /translation="MTDPVREKRARIAKLVGLGLAAGYGLFALAMVLFVIGLVVGYTD
                     PLVQVIVFSLVIGSIVLAPAIVFNYAVRAADRDDAERGL"
     gene            complement(3293..>3802)
                     /locus_tag="VFP02_09500"
     CDS             complement(3293..>3802)
                     /locus_tag="VFP02_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543306.1"
                     /translation="APDEPLLVHPVRAIERKNVPAALALAEAVGGTYWLPGGAEEGYG
                     PILEGLLAGAACRVIHRAWTGRSDLYAAADAVLFPSTWEGFGNPPVEAALHGRQAAVG
                     EYPVAEELRVLGFRWLPADDPAPLAAWLATPDPAVLAHNRSVAEAHMSLAAQTESLRT
                     LLDQAGWLP"
BASE COUNT          540 a         1383 c         1392 g          487 t
ORIGIN      
        1 gatccgggac cgggacctgc tcctcaacgg cgagcgggtc ctcatccggg gggtgaaccg
       61 ccacgaccac cacccggacc ggggcaagac cctcacccgc gaggacatgg agcgcgatgt
      121 cgtgctcatg aagcgcttcg gcttcaacgc cgtgcgctgc gcccactacc cgaacgaccc
      181 gaccttcctg gacctgtgtg acgagtacgg cctgtgggtc atcgacgaag ccaacgtcga
      241 gagccacgcc cgccagctgg ctctggccca cgacccccgc tatcacgccg ccatcgtcga
      301 gcgggtcgtg cgcatggtcc ggcgagaccg caaccacccc agcgtcatcg cctggtcgct
      361 gggcaacgag tccggatacg gcatcgccca tgacgccgcg gcggcctggg tccggcggac
      421 cgacccgagt cgcttcctcc actacgaggg ggcgatcatg cacagctggc tgaccgagcc
      481 gggggtgggg gcggccgtca ccgacgtggt ctgcccgatg tacccccaga tcgccgacat
      541 cgtggcctgg gcggagcggg gtgacgaccg acgacccatg atcctctgcg agtaccagca
      601 cgccatgggc aacagcaacg gctcgctggc cgactactgg gccgccttcg agtccacgcc
      661 cggactgcag ggcgggttca tctgggactg gatcgaccag ggcctgacct ggctggacgc
      721 cctcggccgt ccgtacttcg cctacggcgg ggcgttcggc gacgagccca acgacgccga
      781 cttctgcggc aacgggatgg tgggctcgga ccgcgtcccc catcccgcct gcttcgagca
      841 cgccaagctg gcccagccgg tggaggtgga agccctcaat ctgcacgagg gtcgttttcg
      901 gctccgcaac cggcggacgt tcaccggcct gaacgacgtc gacgggcact ggacgctcac
      961 cgtcgacggt gaagcggtgg ccgagggccg ggtcctcgtc cccgccgtcc cgccgctcgg
     1021 gacggccgag atcgatgtcc cgatcgagcg ccccaccagc ctcggccagg gccagcggtg
     1081 ccacctgctg ctgagcttcg tcctcggcga gggacaggcg tgggccccga ggggcttcga
     1141 ggtggcctgg tcccagttcg agatcccgtg ggtggcggct cagccggcgc cgatcgcccg
     1201 cgcccgccgc gtgcgcatcg ccgtcgacgt ggacgagggg accgtgtcgg cgggtgacct
     1261 gctcgtgacc agccccaccg cgtgcatctg gcgggcgccg acccagaacg acggcctccg
     1321 ggtgggcccc atggccgagc gggccccggg ggtccgccgg cgctggctgc gctggggtct
     1381 ggatcgcctt atcaacgagc tggtcagctc ggtgccgcgg gacgagggga tcaacccgtc
     1441 cctcaccgtc caccgccgcc tctcgggggt cgaccccgac gtgcgcatcg accaccggca
     1501 ggtggccatg ctcgtcggcg gcaccctcgt cttcgacgag gagatccgga tccccgaggt
     1561 cctggacgac ctcccccgcg tcggcatcac gttcaccgcg gcggccggcc tcgaacacct
     1621 gacctgggcg ggcctcggcc cgcacgagtc ctatcccgac cgccgggtgg gcgcccgctt
     1681 cggccggtgg acgagcaccg tcaccgacca gtacgtcccc tacctgttgc cccaggagca
     1741 cggggcccac gccgacaccc gtcgcttcac gctgaccgac gacgagggcc atggcctcgc
     1801 cgtctcggcc caggagccct tctcgttctc ggtctcgcac ttctccgccg acgatctcca
     1861 ctccgccgcg gcgaccgcgg acctccagcc ccgggacgag gtgatggtcc acgtcgacgt
     1921 cgcccaacgg ggcctgggaa cggccgcgtg tggcccggac accctgcccg agtaccgggt
     1981 ggccggcggc accttccgct ggcgctggac cctcaccccg ctctgacccc aagggttacg
     2041 cgccggggcc ggggcgttcc atccagaggg tcgggtcgag gagggggacg aagcccacgt
     2101 cggcgtagac gccgtgggcg tcggcggtgg ccaggaggag gcgcttcatc ccggccagat
     2161 cggggtcggc gacgatcgcc tcgatcagtt ggtggcccag gccctggccc cggtgcgccg
     2221 ccacgacgaa gaggtcgcag atccaaccga aggtgacgcc gtcggtcacg acccgggcga
     2281 aggcggccat cgtgccgtcc tcggccacca gggtgtagag gcggctggcg gccagcgacc
     2341 gttcgaccac ggcccgaggg cgaccgaggg accagtaggc ctcgtcgtgg aggaacgtgg
     2401 ccaccacgtc cacgtccacg tcggccgggt ccgtggacag ccggaaggtc acgggccgcg
     2461 gacgccccgc agcccggccc aagccagctc ggccacctgg gcggccagca cggcggggtc
     2521 ctcacgcagg ccgtcgacca gccaccgccg gctggccccc tcggccaggc cgaccaggcc
     2581 gtgggccagc agggcctggt gggcgggggc gatgccctcg atgtcgatga ggccggcgat
     2641 ggcggtggcg atcgacgcct cggtggccgc ggcctggcgg gcgaactcgg ggtcccggcg
     2701 ggtgtcccca ccgaacagca cctggaaggc gtcctggtcc ccggcaacga agcggaagta
     2761 ggcgccgaag ccggcctcca cctgctcgcg gggcccgggc gcgccggccg cggccttggc
     2821 gatggtctcc tccaggcgcc cgccgacgtc ctcgaggacc tcgcggtaca ggtcccgctt
     2881 ggaccggaag tgctggtaga gcacgggctt ggtgaccccg gcggcgtcgg cgatgtcgtt
     2941 catggcggtg gcgtggaagc cccgctcggc gaagacggtg cgggccgtgt cgagcagctg
     3001 gcgccggcgg cgggcggccg ggaggcgggg ggtcgtgctc atggtcagag gccccgttcc
     3061 gcgtcgtcgc ggtctgcagc ccgcaccgcg tagttgaaga cgatggcggg ggccaggacg
     3121 atcgagccga tgacgaggga gaacacgatg acctgcacca ggggatcggt gtagccgacg
     3181 acgaggccga tcacgaacag gaccatggcg agggcgaaca ggccgtaccc ggccgcgagg
     3241 ccgaggccga ccagcttggc gatgcgggcc cgcttctcgc gcacggggtc ggtcacggca
     3301 gccagcctgc ctgatccagc agggtccgga gcgactcggt ctgggcggcc agcgacatgt
     3361 gggcctcggc caccgaccgg ttgtgggcca ggactgccgg gtcgggggtg gccagccagg
     3421 cggcgagcgg ggccgggtcg tcggccggaa gccaccggaa gccgaggacc cgaagctctt
     3481 cggcaaccgg gtactcgccg acggcggcct gccgaccgtg gagagcggcc tcgaccggcg
     3541 ggttgccgaa gccctcccac gtcgacggga acaggacggc gtcggccgcg gcatagaggt
     3601 ccgaccgacc ggtccaggcc cgatggatca cccggcaggc cgcccccgcc agcaacccct
     3661 cgaggatggg cccgtagccc tcctcggccc cgccgggcag ccagtaggtg cccccgacgg
     3721 cctcggccag ggccagcgcg gcggggacgt tcttgcgctc gatggcccgc acggggtgga
     3781 ccaggagggg ctcgtcgggg gc
//
LOCUS       DASTFV010000519         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1912651, whole genome shotgun sequence.
ACCESSION   DASTFV010000519 DASTFV010000000
VERSION     DASTFV010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912651"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFP02_09505"
     CDS             <1..208
                     /locus_tag="VFP02_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9543307.1"
                     /translation="KLLGVEHEQRIQELGLGLLGPAGAVDEGEGAAWIGGYLGNRCLS
                     IAGGTSEIQRNVIAERLLGLPKDP"
     gene            222..851
                     /locus_tag="VFP02_09510"
     CDS             222..851
                     /locus_tag="VFP02_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET9543308.1"
                     /translation="MRRTIAFDLDGTLSDWEAAIRLAEPEHGEALLEALDGLHPREGG
                     ELVDGAHYLLFVRGEDIWADVLGVADGAVEAAERFMAGFRAVAFPEVDAVLARLTADF
                     RVVLVTNSPRAHAALDDMGLAHHFETVVELPPELRKPRPEGFWHAAEQLGVEPADLVY
                     VGDSYRCDVQAGVAAGVRPIWVDRWGTKLPVPDGVTRITSLEALPAALA"
     gene            862..1836
                     /locus_tag="VFP02_09515"
     CDS             862..1836
                     /locus_tag="VFP02_09515"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543309.1"
                     /translation="MAQVPTVTGSIDTAELGPTLMHEHVFVLSPEIIQNYGDTYWDAE
                     ERIADAVSKLNDLASRGIRSIVDPTVIGLGRYLPWVQRVAEQVELQIVAATGLYTYND
                     VPFFFHYRGLLFDTDPDPMVTFFVKDITEGIAETGVKAGIIKCATDADGLTPGVERVL
                     RACARTHRETGVPITTHTDAGSFRGRDQQRVFAEEGVDLTRVVIGHSGDSQDLDYLRE
                     LMDQGSMIGMDRFGIDVYLPTSDRVQVVADLCAQGYADRMVLAHDAACYMDWFTPELI
                     ETMTPNWHFNHISDDVLPALLDRGVTQDQIDQMLVGNPRRLFEMTGAY"
     gene            1893..2348
                     /locus_tag="VFP02_09520"
     CDS             1893..2348
                     /locus_tag="VFP02_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9543310.1"
                     /translation="MATWKDVETADPAFAARVRALFAAHKHKTIATLRADGSPRISGI
                     EAEFEDGQLTFGSMPGARKGADLVRDPRFALHSASEDPPEDDPSGWSGDAKVAGRAVL
                     VGDLEGDISGQGFRAELDEVVLTRLNDAGDRLLIEIWRPGEPLERVERE"
BASE COUNT          380 a          910 c          881 g          358 t
ORIGIN      
        1 caagctcctc ggggtcgagc acgagcaacg catccaggag ctgggcctcg gcctgctcgg
       61 cccggccggg gccgtcgacg agggggaggg ggcggcctgg atcggtggct acctcggcaa
      121 tcgctgcctg tccatcgccg gtggcaccag cgagatccag cgcaacgtga tcgccgaacg
      181 cctcctcggc ctccccaagg atccctagaa gggacccgga catgcgccgc acgatcgcct
      241 tcgacctgga cgggacgctg tcggactggg aggcggcgat ccgcttggcc gagcccgagc
      301 acggcgaggc gctgctcgag gccctcgacg gccttcatcc ccgcgagggc ggggagctgg
      361 tcgacggggc ccactacctg ctcttcgtgc ggggggagga catctgggcc gacgtgctcg
      421 gcgtcgccga cggggcggtc gaagcggccg agcgattcat ggccgggttc cgggccgtcg
      481 ccttcccgga ggtcgacgcc gtgctggccc ggctgaccgc ggacttccgg gtcgtgctgg
      541 tgaccaacag cccccgagcg cacgccgccc tcgacgacat gggcctggcc caccacttcg
      601 agaccgtcgt ggagctcccg cccgagctgc gcaagccccg accggagggg ttctggcacg
      661 cggccgagca gctcggcgtc gagcccgccg acctggtcta cgtgggggac agctaccgct
      721 gcgacgtcca ggccggggtc gctgccggcg tgcgcccgat ctgggtcgac cgctggggca
      781 cgaagctccc ggttcccgac ggcgtcaccc ggatcacgag cctggaggcc ctgcccgccg
      841 cactcgcgta aattccgcgg catggcccag gtacccaccg tcaccggttc gatcgacacg
      901 gccgagctcg gccccacgct gatgcacgag cacgtcttcg tgctcagccc cgagatcatc
      961 cagaactacg gcgacaccta ctgggacgcg gaggagcgca tcgccgacgc cgtgagcaag
     1021 ctcaacgacc tggcgtcccg cggcatccgc agcatcgtcg acccgacggt catcgggctc
     1081 gggcgctacc tgccctgggt ccagcgggtg gccgagcagg tcgagctcca gatcgtggcc
     1141 gccaccggcc tctacacgta caacgacgtc cccttcttct tccactaccg gggcctgctc
     1201 ttcgacaccg atcccgaccc gatggtcacc ttcttcgtga aggacatcac cgagggcatc
     1261 gccgagacgg gcgtgaaggc cgggatcatc aagtgcgcga ccgacgccga cggcctgacc
     1321 cctggcgtgg agcgggtgct gcgggcctgt gcccggaccc accgggagac cggcgtcccc
     1381 atcaccaccc acaccgacgc cggcagcttc cgggggcgcg atcagcaacg ggtcttcgcc
     1441 gaggaggggg tggacctgac ccgagtcgtc atcgggcact ccggtgactc ccaggacctg
     1501 gactacctgc gggagctcat ggaccagggc tcgatgatcg gcatggaccg gttcggcatc
     1561 gacgtctacc tgccgaccag cgaccgggtg caggtggtgg ctgacctgtg tgcgcagggc
     1621 tacgccgatc gcatggtcct ggcccacgac gccgcctgct acatggactg gttcacgccc
     1681 gagctgatcg agaccatgac cccgaactgg cacttcaacc acatctccga cgacgtgctc
     1741 cccgcgctgc tggacagggg cgtcacccag gaccagatcg accagatgct cgtgggcaac
     1801 ccccgccgcc tcttcgagat gaccggcgcc tactgaccgc ggagccacct gccggcgtgg
     1861 cgtcgcgcgg gcggtccggg catgatctct gcgtggccac ctggaaggac gtggagaccg
     1921 ccgacccggc gttcgcggcc cgggtgcgtg cgctcttcgc cgcccacaag cacaagacga
     1981 tcgccacgct gcgtgctgac gggtcgcccc gcatctcggg catcgaggcg gagttcgagg
     2041 acggccagct cacgttcggc tccatgccgg gagcgcgcaa gggagccgat ctcgtccgcg
     2101 accctcggtt cgccctgcac agcgcctcgg aggacccgcc cgaggatgac ccgtccggct
     2161 ggagcggcga cgccaaggtc gccggtcggg ccgtgctcgt cggcgacctc gagggcgaca
     2221 tctccggtca ggggtttcga gccgagctgg acgaggtggt cctcacacgg ttgaacgacg
     2281 caggcgaccg cctgctcatc gagatctggc ggcccgggga acccctggag cgcgtggaac
     2341 gagagtgatc gactcggccc cgccgaatca ggcgtccgat cggcggagca cgtcgcggag
     2401 ggcgaacttc tggaccttgc ccgtggcggt gcgggggagg tcggccacct cccggaccac
     2461 ctctggccac ttctgacggg ccaggccggc ggcctcgagg tgggcccgga tggcctcgag
     2521 gtcgggggc
//
LOCUS       DASTFV010000520         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1912928, whole genome shotgun sequence.
ACCESSION   DASTFV010000520 DASTFV010000000
VERSION     DASTFV010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912928"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFP02_09525"
     CDS             <1..392
                     /locus_tag="VFP02_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9543311.1"
                     /translation="LSSLEYLASFLVIASVMVLPIALMAPGPFDPGAEGWWIVIVVAV
                     LSGGVGHFLMNFAHPHIPLYLASLLTLAVPVMSTTVALIFLDESINALQVLGMMVVLT
                     AIGLVVLRSERGKDVDSAVLLEAEPPA"
     gene            complement(407..1180)
                     /locus_tag="VFP02_09530"
     CDS             complement(407..1180)
                     /locus_tag="VFP02_09530"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9543312.1"
                     /translation="MLASGMGKDVAAYDVIGLGYATWRRPDRRIAAGLQEALGDCRRV
                     INVGAGTGSYEPADREVIAVEPSRAMIAQRPSAMAPCIQATAEALPFPDDCFDAALAI
                     LTIHHWQRLAAGLADLNRVAVSRRIVLTWDQRVMENDFWLLRDYLPGLGRRLRATAVP
                     VDHVAELIGADVILPVPIPDDCTDGFFGAYWRRPSLYLDHRVRSAISAFALAEVEHYR
                     EGLARLDADLRSGAWHRRYGDLFDVSTYDLGYRLVVAAN"
     gene            complement(1249..2343)
                     /locus_tag="VFP02_09535"
     CDS             complement(1249..2343)
                     /locus_tag="VFP02_09535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9543313.1"
                     /translation="MEFTFSDDQDALRETVRAFLGKESPSAYVRAMLEDEVGTSDAVW
                     NQLVELGWTGLLVPEDLGGLGMGLVDMAVVLEEMGQVPFPGPYLSSAVFATIAARHLG
                     LDEQLRSLAAGATRGTIAVDELGHGDPVDRIRTRAKRKGGHWRLHGLKPVVLDGSSAD
                     WALVPARTQEGIGTFLLEAPKGETVPTWDHTRRVARLDLDGRAAERVGPDGDHTALWR
                     RIVDDANVALCCDLLGVMEQAHHLAVEYAKVRVQFDRPIATFQVIKHKAVDMLHAIEL
                     TRVGTHYAAWTSDTEDERRAEAVAMAMGFASESANEVCAESIQIHGGVGFTWDCDAHL
                     FYRRAKQDDLLLGSGGWHRAKLADQVLTPA"
     gene            2398..>2988
                     /locus_tag="VFP02_09540"
     CDS             2398..>2988
                     /locus_tag="VFP02_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9543314.1"
                     /translation="MDFAYTPEDEAFRTELREWLDEHLPKFLAEWSDEDPQTEDTSGS
                     HDYGGLLRSMERRKAWQRTLNEGRWAAINWPTEWGGRDATVMQSVIYSEEMAKARTPG
                     IFNANGLWQIGPMIIHWGTDEQKNTWIPNILNADDHWCQGFSEPEAGSDLANLRTTAV
                     LDGDDYVLNGQKIWISTAHIAKWGLFLVRTDPDAIAE"
BASE COUNT          457 a         1051 c         1053 g          427 t
ORIGIN      
        1 cgctcagctc gctcgagtac ctggcgtcct tcctcgtgat cgcatcggtg atggtcctgc
       61 ccatcgccct gatggcgccc ggccccttcg accccggggc cgagggctgg tggatcgtga
      121 tcgtcgtcgc cgtgctgtcg ggcggcgtcg ggcacttcct catgaacttc gcccaccccc
      181 acatcccgct gtacctggcc agtctcctga ccctggcggt ccccgtgatg tcgacgacgg
      241 tcgccctgat cttcttggac gagtcgatca acgccctcca ggtcctcggc atgatggtcg
      301 tgctcaccgc catcggcctc gtcgtcctcc gcagcgagcg gggcaaggac gtcgacagtg
      361 ccgtcctcct cgaggccgaa cctcccgcct gaccgttcct ctggggtcag ttggcggcga
      421 cgaccaggcg gtagccgagg tcgtaggtgg agacgtcgaa gaggtcgccg tagcggcggt
      481 gccaggcgcc ggagcggagg tcggcgtcca ggcgggccag accttcccgg tagtgctcca
      541 cctcggccag ggcgaaggca gagatggcgg aacgcacccg gtggtcgagg tacagcgacg
      601 gacggcgcca gtaggccccg aagaacccgt cggtgcagtc gtcggggatg ggcacgggga
      661 ggatgacgtc ggccccgatc agctcggcga cgtggtccac ggggacggcc gtggcccgca
      721 ggcgccggcc caggccgggc aggtagtccc gcaggagcca gaagtcgttc tccatcaccc
      781 gctggtccca ggtcagcacg atgcgacggg acaccgcgac ccggttcagg tcggccaggc
      841 cggcggccag ccgctgccag tggtggatgg tgaggatggc cagagcggcg tcgaagcagt
      901 catcggggaa gggcagggcc tccgcggtgg cttggatgca cggcgccatg gccgagggcc
      961 gctgggcgat catcgcccgg gacggctcga cggcgatcac ctcacggtcg gccggctcat
     1021 aagacccggt gccggcgccg acgttgatca cccgccggca gtcgccgagg gcctcctgca
     1081 gcccggcggc gatacgacgg tcgggtcggc gccaggtggc gtagccgagg ccgatgacgt
     1141 cgtaggcggc cacgtccttg cccatccccg aagccaacat gcgacgcaga gcgtaggttc
     1201 tcgccaccct ccgtcctgaa tccccccgtc cagcacgacg gggcagggct aggcgggggt
     1261 gaggacctgg tcggcgagct tggcgcggtg ccaaccgccg gagccgagga ggaggtcgtc
     1321 ctgcttggcc cggcggtaga agaggtgggc gtcgcagtcc caggtgaagc ccacgccgcc
     1381 gtggatctgg atcgactcgg cgcagacctc gttggccgac tcggaggcga agcccatggc
     1441 catggccacg gcctcggccc gccgctcgtc ctcggtgtcg gaggtccacg ccgcgtagtg
     1501 ggtgccgacc cgggtcagct cgatggcgtg gagcatgtcc acggccttgt gcttgatgac
     1561 ctggaaggtg gcgatcggcc ggtcgaactg gacgcggacc ttggcgtact cgacggccag
     1621 gtggtgggcc tgctccatca cccccagcag gtcacagcag agcgcgacgt tggcgtcgtc
     1681 gacgatgcgc cgccagaggg cggtgtggtc gccgtcgggg ccgacccgct cggcggcccg
     1741 gccgtccagg tcgagacggg ccacccgccg cgtgtggtcc cacgtcggca cggtctcgcc
     1801 cttcggcgcc tccaggagaa aggtgccgat cccttcttgg gtgcgggcgg ggacgagggc
     1861 ccagtcggcc gacgatccgt cgagcacgac cggcttcagc ccgtgcaggc gccagtgccc
     1921 gcccttgcgc ttggcccggg tgcggatgcg gtcgaccggg tcgccgtggc ccagctcgtc
     1981 gacggcgatg gtccctcggg tcgctcccgc ggccagcgac cgcagctgct cgtccagccc
     2041 gaggtgacgg gcggcgatcg tggcgaacac ggccgaggac aggtagggcc cggggaaggg
     2101 gacctgcccc atctcctcga gcacgaccgc catgtcgacc agccccatgc cgaggccccc
     2161 gaggtcctcg ggcaccagga ggccggtcca gcccagctcc accagctggt tccagacggc
     2221 gtccgaggtc ccgacctcgt cctccagcat cgcccggacg taggccgagg ggctctcctt
     2281 gcccaggaag gcccgcacgg tctcccgcag ggcgtcctgg tcgtcgctga aggtgaattc
     2341 catccgtccc cttgacctga caccggggtc aggtacggac agtagtgttc gcccccgatg
     2401 gacttcgcct acacgccaga ggacgaggcc ttccgcaccg agttgcggga gtggctggac
     2461 gagcacctgc ccaagttcct ggccgagtgg agcgacgagg acccccagac cgaggacacc
     2521 tccggcagcc acgactacgg cggactcctg cgctcgatgg agcgccgcaa ggcgtggcag
     2581 cggaccctga acgaggggcg ctgggccgcc atcaactggc ccaccgagtg gggcggccgg
     2641 gacgccacgg tgatgcagag cgtcatctac tccgaggaga tggccaaggc ccgcaccccc
     2701 ggcatcttca acgccaacgg cctgtggcag atcgggccca tgatcatcca ctggggcacc
     2761 gacgagcaga agaacacctg gatccccaac atcctcaacg ccgacgacca ctggtgccag
     2821 gggttcagcg agcccgaagc cggcagcgac ctggccaacc tccgtaccac cgcggtactc
     2881 gacggcgacg actacgtcct caacggccag aagatctgga tctccacggc ccacatcgcc
     2941 aagtggggcc tgttcctcgt ccgcaccgac cccgacgcga tcgccgag
//
LOCUS       DASTFV010000521         3604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1922034, whole genome shotgun sequence.
ACCESSION   DASTFV010000521 DASTFV010000000
VERSION     DASTFV010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922034"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFP02_09545"
     CDS             <1..612
                     /locus_tag="VFP02_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9543315.1"
                     /translation="HGVSPVARDLTEAGHRLGRAGGWGCYLGYSLGGRVALHLALAQP
                     DLVTALVVIGAHPGIEDGDEREARRRADDELADHLEAVGLDAFLDEWLAQPLFASLPP
                     EADQRSERLTNDPGALAATLRNLSTGRQVPLWEHLPKLSMPVLILAGEHDETYRRLGR
                     QAAATIGENASFETVPDAGHAAHLEQPGHVAQRVRAFLTATGV"
     gene            complement(613..1476)
                     /locus_tag="VFP02_09550"
     CDS             complement(613..1476)
                     /locus_tag="VFP02_09550"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HET9543316.1"
                     /translation="MNPWVAGARPRTLPAAVVPVAVGTAVAVGEGIVVWRLVAALIVA
                     LAIQVGTNFANDYQDGVRGTDDVRVGPVRLVASGLASASDVRTAALASFGVAAVFGLA
                     LAAVVGWELVLVGLLSFAAGWLYTGGPKPYGYLGLGEVFVFVFFGLVATVGSFYVQTS
                     EVTALAVCAAVPVGLLATALLVVNNLRDIPTDTEAGKRTLAVRLGDRTTRIVYVTMLV
                     DVFLLVIVLAVTARMPALLGLVGIVFAIPPIRRVRAGAKGPELIPVLGATGRAQLVTG
                     LLLALGLALGG"
     gene            complement(1499..2338)
                     /gene="menB"
                     /locus_tag="VFP02_09555"
     CDS             complement(1499..2338)
                     /gene="menB"
                     /locus_tag="VFP02_09555"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HET9543317.1"
                     /translation="MRPAADWQAAGQFRDIRYETADGMAKITICRPEVRNAFRPQTLF
                     ELQDAFNRARDDERVGVIIFTGEGDLAFCSGGDQRIRGDDGYLGDDEVAQKGIGRLNV
                     LDLQIQIRRTPKPVIAMVAGYAIGGGHVLHVVCDLTIAADNARFGQTGPRVGSFDGGY
                     GSGLLARSIGQKRAREIWFLCRQYTAEEAYEMGMVNTVVPLAALEEETVAWCREMLAH
                     SPLAMRMIKASMNAADDGLAGIQQLAGDATLLYYQTDEAQEGRDAYVNKRRPDFDQFP
                     KRP"
     gene            complement(2378..3121)
                     /locus_tag="VFP02_09560"
     CDS             complement(2378..3121)
                     /locus_tag="VFP02_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9543318.1"
                     /translation="MQIDLEGRVALVTGASKGIGRSIAATLAASGAKVMLSSRKQDAL
                     DEAAAGMDGEVAAFAANAGDPDEAEACVAATVERFGRVDVLVNNAATNPYMGPAIDID
                     LARYDKTFQVNLRGPLVWTQLAHRASMAEHGGSVINIASVGGLSVEPSIGIYNTTKAG
                     IIHLTKTLAAELGPKVRVNAIAPGLVKTDFARALWEAHEEALARRLPTKRLGEPEDVA
                     GAALFLASDLSSWITGHTLVVDGGALVTA"
     gene            3184..>3604
                     /locus_tag="VFP02_09565"
     CDS             3184..>3604
                     /locus_tag="VFP02_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543319.1"
                     /translation="MSHEQPEDRRARRLAAVRAERGSEERARQIAELEAEKAKPKVRA
                     RSATPSVRKSPESSSTFFGYPWAEWFEMRDAGRARLNTCAAERQLITYGDLWAAIAET
                     VGHELGSQHLALPRLLRDIGDVAIKDGEPNPMALAVQS"
BASE COUNT          545 a         1262 c         1303 g          494 t
ORIGIN      
        1 cacggggtct cgcccgtcgc ccgggacctg acggaggccg gccaccgcct cggccgagca
       61 gggggctggg gttgctatct cggctactcg ctcggcggtc gcgtggccct gcacctggcc
      121 ctggcccagc ccgacctggt caccgccctc gtcgtcatcg gggcccatcc cggcatcgag
      181 gatggtgacg agcgggaggc ccggcgtcgg gccgacgacg agttggctga ccacctcgag
      241 gccgtcgggc tcgacgcatt cctcgacgag tggctggctc agcccttgtt cgcctcgctg
      301 ccaccggaag ccgaccagag gagcgagcga ctgacgaacg atcccggggc gctggcggcg
      361 accctgcgca acctcagcac cggccggcag gtcccgctct gggagcacct ccccaagctc
      421 tcgatgccgg tgctcatcct ggccggcgag cacgacgaga cctatcggcg cctcggtcgc
      481 caggccgcgg ccaccatcgg ggagaacgcc agcttcgaga ccgtgcccga cgcgggccac
      541 gccgcgcacc tcgaacagcc gggccacgtc gcccaacggg tccgcgcctt cctcaccgcc
      601 actggcgtct gatcagccgc cgagggccag ccccaacgcc aggaggaggc ccgtgaccag
      661 ttgcgccctg ccggtcgcac cgaggacggg gatgagctcg ggccccttgg cccctgcccg
      721 cacccgccgg atcgggggga tggcgaacac gatcccgacc aggcccagca gggccggcat
      781 ccgggccgtg accgcgagca cgatcacgag caggaagacg tcgacgagca tggtcacgta
      841 cacgatccgc gtcgtccggt cgccgaggcg cacggccagc gtgcgcttgc ctgcttcggt
      901 gtcggtcggg atgtcccgca ggttgttgac caccagcagc gcggtggcca acaggcccac
      961 tgggaccgcg gcacagacgg ccagcgcggt cacctcgctg gtctgcacgt agaaggaccc
     1021 gaccgtggcc accaggccga agaagacgaa gacgaagacc tctcccaggc cgaggtagcc
     1081 gtagggcttg ggaccacccg tgtagagcca gcccgcggcg aaggacagga gcccgacgag
     1141 cacgagctcc cagcccacca cagcagccag ggccagcccg aacacggcgg ccacaccgaa
     1201 ggacgccagg gcggcggtgc gtacgtccga ggccgatgcc aggcctgagg cgacgaggcg
     1261 cacgggtccg acgcggacgt cgtcggtgcc ccgcaccccg tcctggtagt cgttggcgaa
     1321 gttggtcccc acctggatgg ccagggccac gatcagggcg gcgacgagcc gccacaccac
     1381 gattccctcg ccgaccgcga ccgcggtgcc gacggccacg gggacgaccg cagcgggcag
     1441 ggtccggggt cgggcccccg ccacccaggg gttcatcacg gccgcgccgc gggtgccgtc
     1501 aaggccgctt ggggaactgg tcgaagtcgg gacggcgctt gttcacgtag gcgtcccggc
     1561 cttcctgcgc ctcgtcggtc tggtagtaga gcaacgtggc gtcgcccgcc agctgctgga
     1621 tgccggccag gccgtcgtcg gcggcgttca tcgaggcctt gatcatgcgc atggccaacg
     1681 gggagtgggc cagcatctcc cgacaccagg ccaccgtctc ctcctcgagg gctgcgaggg
     1741 ggaccacggt gttcaccatt cccatctcgt acgcctcctc tgcggtgtac tggcggcaga
     1801 ggaaccagat ctcccgggct cgcttctggc cgatgctgcg ggccagcagg cccgagccgt
     1861 agccgccgtc gaaggacccg acgcggggcc cggtctgacc gaagcgggcg ttgtcggcgg
     1921 cgatggtcag gtcgcacacg acgtgcagga cgtggccccc gccgatggcg tagcccgcca
     1981 ccatggcgat gacgggcttg ggggtgcgcc ggatctggat ctggaggtcg aggacgttga
     2041 gccgtccgat ccccttctgg gccacctcgt cgtcgccgag gtagccgtcg tcaccccgga
     2101 tgcgctggtc gccgccggag cagaaggcca ggtcgccctc gccggtgaag atgatcacgc
     2161 cgacccgctc gtcgtcccgg gcccggttga aggcgtcctg gagctcgaag agcgtctggg
     2221 gccggaaggc gttccggacc tccggacggc agatcgtgat cttggccatg ccgtcggccg
     2281 tctcgtaccg gatgtcccgg aactggcctg cggcctgcca gtcggcagcc ggtcggatgg
     2341 tggtctcagt ggacgtcacg gccacagagt gtgccgctca ggcggtgacc aacgcacctc
     2401 cgtcgacgac gagggtgtga ccggtgatcc aggaggacag gtcgctggcc aggaagaggg
     2461 cggcgccggc gacgtcctcg ggctcgccca ggcgcttggt cggcagtcgc cgggccaggg
     2521 cctcctcgtg ggcttcccag agggcgcggg cgaagtcggt cttgaccagg ccgggggcga
     2581 tggcgttgac ccggaccttc gggcccagct ccgcggccag ggtcttggtg agatggatga
     2641 tccccgcctt tgtcgtgttg tagatgccga tggacggctc gacggagagc ccgccgaccg
     2701 aggcgatgtt gatgaccgag ccgccgtgct cggccatcga tgcccggtgg gccagctgcg
     2761 tccagaccag gggcccgcgc aggttcacct ggaacgtctt gtcgtagcgg gccaggtcga
     2821 tgtcgatcgc cgggcccatg tacggattgg tggcggcgtt gttgaccagc acgtcgaccc
     2881 tgccgaaacg ctcgacggtg gccgccacgc atgcttcggc ctcgtccgga tcaccggcgt
     2941 tggcggcgaa ggcggcgacc tcaccgtcca tgccggccgc ggcctcgtcg agggcatcct
     3001 gcttgcggga cgagagcatg accttcgccc ccgacgccgc gagggtggcg gcgatggacc
     3061 gcccgatgcc cttcgaagcc ccggtgacga gcgcgacccg tccttcgaga tcgatctgca
     3121 tgccggcgaa cgtagccgtc gggctgggta gggtccggcg ccgatcgacg aggagcccag
     3181 accatgagcc acgaacaacc cgaggatcga cgggcgcgcc ggctggccgc cgttcgggcc
     3241 gagcggggat ccgaggagcg ggcccgccag atcgcggagc tggaggcgga gaaggcgaag
     3301 ccgaaggtgc gggcccggtc ggcgacgccg agcgttcgca agtcaccgga gtccagttcc
     3361 accttcttcg gctacccgtg ggccgagtgg ttcgagatgc gcgacgccgg ccgggcccgg
     3421 ctgaacacgt gcgccgccga gcgacagctg atcacctacg gcgatctctg ggcggcgatc
     3481 gccgagacgg tgggtcacga gctggggagc cagcacctcg ccctcccccg tctcctgcgg
     3541 gacatcggag atgtggcgat caaggacggc gagccgaacc cgatggcact ggccgtccag
     3601 agcg
//
LOCUS       DASTFV010000522         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1926690, whole genome shotgun sequence.
ACCESSION   DASTFV010000522 DASTFV010000000
VERSION     DASTFV010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926690"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2972
                     /locus_tag="VFP02_09570"
     CDS             <1..2972
                     /locus_tag="VFP02_09570"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HET9543320.1"
                     /translation="AFGLRVDPALVEVLVGEVAGEPGALPLLSHALRSTWERRDTRTL
                     ALDAYRATGGLRAAIATTAENVYGQLDADDQDLARRTFLALTEPGEGTEDSRRWATLI
                     ELTPAGGANRVEGLLDAMAAERLVSRDEGRVAVAHEALIREWPRLRGWLDEDREGLRL
                     HRDLARSAAAWGELGREPAELYRGPRLAAALEWAARTDDLSTLEAAFLAASRAEQERG
                     ERARARTTRRLRGLLAGVGVALVVAVVAALVAYGQRREAADARDRADVARVAAVSRSL
                     MDSQPDVGLLLAATAYDLEDTADTRSTLLSALEAHPLLEGLIYGNDSGLESATFSPDG
                     QVLATATSDGTGTLLWDTETHDQLAALAHEGDLSLDTAISPDGRTLAVPAIHYREDGR
                     PEGRLEIWDLESQALVEVVPSPAGSLSTAAYTADGSRLVTQGGPGADAAPRDVVVVWD
                     TQTWEPVGEPWVLDPGYSGDEVLEVSPDGSLVAMPSPDGGAVGIWRSADRTFVAPVDV
                     HGLVGGEELPVTELAFAPDGQTLAIATDGGSILFVDAATGAEAAEPLRLAESAGTAVE
                     YSPDGTTLAVGRIDGQTQLYDLASREPLGPPLAANASQIKDVSFSADGTLLATTSTDR
                     TGALWRLDGGRSFGIVLSGQDGATTEAVYAPDGSTLLTSSVDGTVAVRDAATGEIERS
                     ITLGGEVESVAVSPDGARAAAGGSGGTVVLFDIDGGDRVEVPVDGVAHQVAFAPDGRT
                     VAVALDHSGAGEVISGAGIGELRFLDAATGDEARAPIDVGEPAVGTGFSPDGGAVVAV
                     TDNNVLRLYDPGSGAEFDPAIENVDAMITSFAFSPDGDRLLVGVTSGAVRPYDATTHE
                     PAGEAFESDLELVFGLAYAPDGSVVAGTNLGFSTASLWDAATGSPIGDVLIGGRVPYT
                     YQTFTLDHLLWARPAFSPDGRHLVTPGFDGATVVWDLDPDAWLAAACDLAGRDLSVDE
                     WARYLPDREPERLCEAG"
BASE COUNT          394 a         1146 c         1145 g          393 t
ORIGIN      
        1 aggcgttcgg cctccgcgtc gacccagcgt tggtcgaggt tttggtcggc gaggtggcgg
       61 gcgagcccgg cgccctaccc ctgctgagcc acgccctgcg gtcgacgtgg gagcgcaggg
      121 acacccgcac gctcgccctc gacgcctatc gcgccacggg cgggctccgg gcggccatcg
      181 ccaccaccgc cgagaacgtc tacggccagc tggacgctga cgaccaggac ctggcccggc
      241 ggacgttcct ggccctgacc gagcccggcg agggcaccga ggactcacgg cggtgggcga
      301 cgctgatcga gctcaccccc gccggtgggg cgaatcgggt ggaggggctg ctcgacgcca
      361 tggccgccga gcgcctggtc agccgggacg agggccgggt ggctgtcgcc cacgaggccc
      421 tgatccggga gtggccacgg ttgcggggct ggctcgacga ggaccgcgag gggctgcgcc
      481 tccaccgcga cctcgcccgg tcggcggcgg cctggggcga gctcggccgg gagcccgccg
      541 agctctaccg gggcccgcgc ctggccgccg cgctcgagtg ggctgcccgc accgacgacc
      601 tgtcgacgtt ggaggctgcc ttcctcgcgg ccagccgggc cgagcaggag cgaggtgagc
      661 gggcccgggc ccgcaccacc cggcggctgc gggggctact ggccggggtc ggggtggcgc
      721 tcgtggtcgc ggtcgtggcc gcgctcgtgg cctacgggca gcggcgggag gcggccgacg
      781 cccgggaccg ggccgacgtg gctcgggtgg cggcggtctc ccgcagcttg atggacagcc
      841 agcccgatgt cggcctcctg ctggcggcga ccgcctacga cctcgaggac accgccgaca
      901 cccgcagcac cttgctcagc gccctggagg cccaccccct cctcgagggg ctgatctacg
      961 ggaacgactc cggcctcgag tcggccacct tcagcccaga cggccaggtc ctcgccacgg
     1021 ccacctcgga cggcaccggc accctcctgt gggacaccga gacccacgac cagctcgcgg
     1081 cgctcgccca cgagggcgac ctcagcctcg acaccgcaat cagccccgat ggtcggacgt
     1141 tggcggtgcc ggccatccac taccgggagg acggccgccc cgagggacgt ctggagatct
     1201 gggacctcga gtcccaggca ctggtcgagg tggtccccag cccggccggc tcgctctcga
     1261 ccgccgccta caccgccgac ggctcccgcc tggtcaccca gggaggcccc ggcgccgacg
     1321 ccgccccccg tgacgtcgtc gtggtgtggg acacccagac ctgggagccg gtgggcgaac
     1381 cgtgggtcct cgatccggga tactccggcg acgaggtcct ggaggtcagc cccgacggga
     1441 gcctggtggc catgccgtcc cccgacggcg gcgccgtcgg catctggcgg agcgccgacc
     1501 gcacgttcgt cgccccggtc gacgtgcacg ggctggtcgg cggtgaggaa ctcccggtga
     1561 ccgagctggc cttcgccccc gacggtcaga cacttgcgat cgccaccgac ggcggctcga
     1621 tcctgttcgt cgacgccgcc accggggcgg aggcggccga gccactgcgg ctggccgaga
     1681 gtgcggggac tgcggtcgag tacagccccg acggcaccac gctggccgtc ggccgcatcg
     1741 acgggcagac acagctctac gacctcgcct cccgggagcc cctggggccg cccctggcgg
     1801 ccaacgccag ccagatcaag gacgtgagct tcagcgccga cggcaccctg ctggcgacga
     1861 ccagcaccga ccgcaccggt gccctctggc gcctcgacgg tggccggtcg ttcgggatcg
     1921 tgctgtccgg ccaggacggg gcgacgaccg aggcggtcta cgccccggac gggtcgacgc
     1981 tgctgacctc gtcggtcgac gggacggtgg cggtgcgcga cgcggccacg ggcgagatcg
     2041 agcggtcgat cacgctcggg ggcgaggtcg aatcggtggc ggtcagcccg gacggggccc
     2101 gggccgcggc gggcggcagc gggggcacgg tcgtgctctt cgacatcgac gggggtgacc
     2161 gggtcgaggt cccggtcgac ggggtggccc accaagtggc gtttgccccg gacgggcgca
     2221 cggtggccgt ggccctcgat cattcgggtg ccggcgaggt gatctctggt gccggcatcg
     2281 gtgagctccg gttcctcgac gcggcgacgg gcgacgaggc cagggcgccc atcgacgtcg
     2341 gcgagcctgc ggtcggcacc gggttctcgc ccgacggcgg agcggtggtg gccgtcaccg
     2401 acaacaacgt gctccgcctc tacgaccccg ggtcgggggc ggagttcgac cctgccatcg
     2461 agaacgtcga cgccatgatc acgtcgttcg ccttcagccc cgacggggac cgcctgctgg
     2521 tgggcgtgac ctccggggcg gtgcggccgt acgacgccac cacccacgag ccggccggcg
     2581 aggccttcga gagcgacctc gagctggtct tcggcctggc ctacgccccc gacggcagcg
     2641 tggtggcggg caccaacctc ggcttctcca ccgccagcct gtgggatgct gccaccggct
     2701 caccgatcgg cgacgtgctc atcggaggcc gggtgccgta cacgtaccag accttcaccc
     2761 tcgatcacct cctgtgggca cgcccggcgt tctccccgga cgggcggcac ctggtcacgc
     2821 cgggattcga cggcgcgacc gtggtctggg acctcgaccc ggacgcctgg ctggccgccg
     2881 cctgcgacct cgccgggcgc gacctcagtg tcgacgagtg ggcccgctac ctcccggacc
     2941 gggagcccga gcgcctgtgc gaagcgggct gaatccgctc acctccgctg tccgcatccg
     3001 ctggcaagcc aggacatgac gggggcgctc ctcggctgtg agcccacgca ccgtgctgac
     3061 ggcggaaagg cgacctcg
//
LOCUS       DASTFV010000523         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1927270, whole genome shotgun sequence.
ACCESSION   DASTFV010000523 DASTFV010000000
VERSION     DASTFV010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1927270"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VFP02_09575"
     CDS             <1..655
                     /locus_tag="VFP02_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET9543321.1"
                     /translation="VVGFPGAKGRIPNFTGAADVARALAELDEWQTADTVKSNPDMPQ
                     LPVRARALADGKLLYLAVPRLREVKPFVLLDPDRLEVKPRAAASIKGSSVHGVPVAVE
                     EIGHVDLIVCGTVAVNRAGVRIGKGGGYSDLEYALGYEAGIIDDTTVIVTTVHGVQLL
                     DEDLPETGHDFRVDIVVTPDEVVRTSAQRRRGRIDWSELDDEQLAAIPALAERAPRR"
     gene            complement(675..1076)
                     /locus_tag="VFP02_09580"
     CDS             complement(675..1076)
                     /locus_tag="VFP02_09580"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9543322.1"
                     /translation="MRDVEVVEAIYAAMAERDFDRLFELVDESVVITQDARLPWGGRH
                     EGHEGLGEFAVKLTGTIDSAVTIESLFAADDEVIQAGRTKGTVVATGVPFDVAEVHRW
                     TVRDGKAVTAHFAIDTAAMLDALAEVGESEG"
     gene            1197..>2120
                     /locus_tag="VFP02_09585"
     CDS             1197..>2120
                     /locus_tag="VFP02_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543323.1"
                     /translation="MTSELADLEHYLDVANKANFHGLPGKTKAESLLAVSRIRNKLDA
                     LDVAVITAFEATKEHVVEGHASCIGWLQHHSRDHGDPAALRRRLARRIRAMPQAQRAL
                     ADGTITSRHIDVLDQARRAVGDDAFALAEEALVDAAVIRRFSDFAELVTYFIHQVSPR
                     DADQREQQRYEDRYGSSSRTLDGDGKIDAWMPALAFTVWQDELERLMEVEYGNDVAEA
                     TDRLGRRPLPSELQRTTRQRRVDAMEEMAKRSAAHAGTDLGPSNITLIVHGSAELVAR
                     LLALLLDEDATIEDLEDLEIPEGSIHELDDGT"
BASE COUNT          348 a          789 c          683 g          300 t
ORIGIN      
        1 cgtggtcggc ttccccggcg ccaagggccg cattcccaac ttcaccggcg ccgctgacgt
       61 ggcgcgcgct ctggcggagc tcgacgagtg gcagaccgcc gacacggtga agtcgaaccc
      121 ggacatgccg cagctaccgg tccgggcccg ggcactcgcc gacggcaagc tgctctacct
      181 ggccgtgccc cgcctgcggg aggtcaagcc gttcgtcctc ctcgaccccg accgcctgga
      241 ggtgaagccc cgggcggcgg cgtccatcaa aggctcctcg gtccacggcg tgccggtcgc
      301 cgtggaggag atcggccacg tcgacctgat cgtctgcggc acggtcgccg tgaaccgggc
      361 cggcgtccgc atcggcaagg gtggcggcta cagcgacctg gagtacgccc tcggctacga
      421 agcggggatc atcgacgaca ccaccgtgat cgtgaccacg gtccacggcg tacagctgct
      481 cgacgaggac ctcccggaga cgggccacga cttccgcgtc gacatcgtcg tgacgccgga
      541 cgaggtcgtg cgcacgagcg cccagcgccg acgagggcgc atcgactggt cagagctcga
      601 cgacgagcaa ctggcggcca tcccggcttt ggccgagcgg gcgccccggc gctgaccctc
      661 gcggcgcaac gggttcagcc ctcggactcc cccacctccg ccagggcgtc gagcatggcg
      721 gccgtgtcga tggcgaagtg cgcggtgacc gccttgccgt cgcggacggt ccaacggtgg
      781 acctcggcca cgtcgaaggg gacgccggtg gccaccacgg tccccttggt gcgaccggcc
      841 tggatcacct cgtcatccgc cgcgaacagg gactcgatgg tgaccgccga gtcgatcgtg
      901 cccgtgagct tcacggcgaa ctcccccagc ccctcgtggc cctcgtggcg gccgccccag
      961 ggcaaccggg cgtcctgggt gatgaccacc gactcgtcga ccagctcgaa cagccggtcg
     1021 aagtcccgtt ccgccatggc ggcgtagatc gcctcgacca cctcgacatc cctcatcgcg
     1081 acgagtatcg caacaaattt ctccgagatc gtccggacgt cggctttgcg tgtcacaccc
     1141 cctgggtagg gttgggtaca ccagttcgac attcggttgt gaggaggtgg tgaccggtga
     1201 ccagcgagct cgcggacctg gagcactacc tcgacgtggc caacaaggcg aacttccacg
     1261 gcctgccggg caagaccaag gccgagtccc tgctggccgt gtcgcggatc cggaacaagc
     1321 tcgacgccct cgacgtcgcc gtgatcaccg ccttcgaggc gaccaaggag cacgtcgtgg
     1381 aggggcacgc ctcctgcatc ggctggctcc agcaccacag ccgggaccac ggcgatccgg
     1441 ccgccctgcg tcggcgtctg gcccgacgga tccgcgccat gccccaggcg cagcgggcat
     1501 tggccgacgg aacgatcacc tcacgccaca tcgacgtatt ggaccaggcc cgccgtgccg
     1561 tcggcgacga cgccttcgcc ctggccgaag aagccctcgt ggacgccgcc gtgatccgac
     1621 ggttctccga cttcgccgag ctggtcacct acttcatcca ccaggtctcc ccccgggacg
     1681 ccgaccagcg ggaacagcaa cgctacgagg accgctacgg ctcgtcgtcg aggaccctgg
     1741 acggcgacgg caagatcgac gcctggatgc ccgctttggc cttcaccgtc tggcaggacg
     1801 aactcgaacg gctcatggag gtcgaatacg gcaacgacgt cgccgaagcc accgaccggc
     1861 tcggccgccg gcccctgccc tccgagctcc agcgcaccac tcggcaacgc cgggtggacg
     1921 ccatggaaga aatggccaaa cgctccgccg cccatgccgg caccgacctc ggcccctcca
     1981 acatcacgtt gatcgtgcat ggctccgccg agctcgtcgc ccggcttctg gctctcctcc
     2041 tcgacgagga cgccaccatc gaggacctcg aggacctgga gatccccgag ggctcgatcc
     2101 acgagctcga cgacggcacc
//
LOCUS       DASTFV010000524         4813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1929988, whole genome shotgun sequence.
ACCESSION   DASTFV010000524 DASTFV010000000
VERSION     DASTFV010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929988"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFP02_09590"
     CDS             complement(<1..326)
                     /locus_tag="VFP02_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543324.1"
                     /translation="MRYMKKKIGLTALCLMLVLSLIGAVDGGVASASDAGSTSSTTVP
                     PTTEAPAASAAPPTTVAPAPPTTDAPASRSTAARRGPDPRWIDYGDCGRGYTACHVAK
                     AHQRDG"
     gene            644..1270
                     /locus_tag="VFP02_09595"
     CDS             644..1270
                     /locus_tag="VFP02_09595"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET9543325.1"
                     /translation="MSDSFSVTDKARKRVLAMRADEPEPDGLALWVEVVGANATEFVY
                     DLYFQGTEHADPDDLVLDLGDLTVVVPDGSRDFLEGATLDMGRDLLNPGLVLDNPNRP
                     PAGAVSPEMAEALGELTGTVEERVRTVLERQINPAVASHGGRAELVEVSESGVVSLRL
                     SGGCQGCGMAAQTLRNGIEVALRDNIPEVTEILDVTDHAAGENPYVQA"
     gene            complement(1512..2720)
                     /locus_tag="VFP02_09600"
     CDS             complement(1512..2720)
                     /locus_tag="VFP02_09600"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET9543326.1"
                     /translation="MPEAVVVAATRSPIGRANKGSLVDMRPDDLATQVVTSLLSRSGV
                     DGTEVEDLIMGCGQPAGESGYNIGRVVAVESGMPDVPGVTVNRYCSSSLQTTRMAAHA
                     IKAGEGDCFISAGVETVSRFMSGMSDGMPGSTNPRFDAARARTAERAQGGAGAWAPPE
                     GLPDIYIAMGETAENVAEYKQVPRERMDEFAALSQQRAVKSQQEGWFDWEITPITLPD
                     GTVVSKDDGPRDGTTKEKLSELKPVFRPDGTITAGNACPLNDGAAAVLVMSDTKARAL
                     GLTPLARIVASGVSGLNPEIMGLGPIEASKQAMARAGMSIGDIDLVEINEAFAAQVIP
                     SADELGIEWDKLNVAGGAIALGHPFGMTGARITATLIHNLREHDKTFGLETMCVGGGQ
                     GMAMIFERLV"
     gene            2838..3272
                     /locus_tag="VFP02_09605"
     CDS             2838..3272
                     /locus_tag="VFP02_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543327.1"
                     /translation="MRVQIGPVSATSVTAWVGYARKALDEIVGAPGGDGVWLPDDTVA
                     TFVHYLDEWEKAAAGVDEVRWTAEIPADEAEYLSHAFFRIASNLANRADERGYTLAPP
                     ESEEFYRALVTSFLTALKEEGHGSDEFSEHLRSFWPGLEDQS"
     gene            3269..3634
                     /locus_tag="VFP02_09610"
     CDS             3269..3634
                     /locus_tag="VFP02_09610"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9543328.1"
                     /translation="MRLLGGVHHVSLNVDDAEVTGRFYEDVLGLTRIERPDFGIPGVW
                     LSAGAVQIHLIEIEGHTAPDGQHFAFQVDDVDAVQARLVEQGVDATAPSSFPGAGRQS
                     FFHDPAGNLIELNQPESPA"
     gene            3631..4197
                     /locus_tag="VFP02_09615"
     CDS             3631..4197
                     /locus_tag="VFP02_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HET9543329.1"
                     /translation="MSFGSAPTRGRLLVAGPPLEDPNFRRTVVFMIEHNEEGALGVVL
                     NRPSPIDLADAVPQWADLAANPPAVFIGGPVEQGAVLGLGRLVAEDPPEGLTPVHAGI
                     GVLDLEADPVRLVGDVGGVRLFTGYSGWGPGQLEDELAMGGWFVVDAEPDDVTTTEPD
                     DLWRAVLRRQPDDDLARLALFPDDLSAN"
     gene            4224..>4813
                     /locus_tag="VFP02_09620"
     CDS             4224..>4813
                     /locus_tag="VFP02_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9543330.1"
                     /translation="MRIEFELAGTPGQPRLLSISGSGSDLRRAPRIQDEAVLAERFEI
                     LAYDHRGLGRSVPDDPHREPTMADYAADAVGLLDHVGWDTCRVLGISFGGMVAQELAV
                     TAAQRIERLVLACTSPGGAGGSSSPLHTLMDMADDERLAQQVAWLADRTVDDPELRAF
                     WTEMMAGLAGSEPTEGERHQLQARSHHDVWDRLPQL"
BASE COUNT          712 a         1715 c         1676 g          710 t
ORIGIN      
        1 ctaccgtccc gctggtgggc cttggccacg tggcaggcgg tgtagccacg gccacagtcg
       61 ccgtagtcga tccaccgggg atcggggcca cgacgcgccg cggtcgaccg ggaagccgga
      121 gcatcggtgg tcggcggggc cggggccacg gtggtcggag gggctgcgct cgcggccggg
      181 gcctccgtgg tcggcggaac cgtcgtcgag ctggtgctgc cggcatcact ggcggacgcc
      241 acgccgccgt cgacggcacc gatcaggctc aggacgagca tcaggcacag tgccgtcagc
      301 ccgatcttct tcttcatgta gcgcacaggg gggtgtgctc cttgggggga ctacccccac
      361 cggacggagg gcgcgcggca gtgctcggag gtgagggaat caccggccgg cccggagggc
      421 agcgactcga gcgggccgtt ggccggtcgt tccgaactcg gaggaccaga cggcgcttct
      481 cttggtgggg tgacggacag gatcaccgtg gtcgtcatga aatcgcaaca ttcagccatg
      541 tgattacagt cacatgaggt tcttgcccgc ttttcctcac atcagaccat gacgatccga
      601 tgacacagac ccaccgcgcc cgccgttccg tatcctggcc ggagtgagcg actccttctc
      661 cgtcaccgac aaggcccgca agcgggtcct ggccatgcgg gccgacgagc ccgagcccga
      721 cgggctggcc ctgtgggtcg aggtggtcgg ggccaacgcc accgagttcg tctacgacct
      781 ctacttccag ggcaccgagc acgccgatcc cgacgacctg gtcctcgacc tcggcgacct
      841 gaccgtggtg gtacccgatg gcagccggga cttcctcgag ggcgccacgc tggacatggg
      901 ccgggacctg ctcaaccccg gcctggtcct ggacaacccc aaccggcccc cggccggtgc
      961 ggtcagcccc gagatggcgg aggccctcgg cgagctgacc ggcaccgtcg aggagcgggt
     1021 ccggaccgtg ctggagcgcc agatcaaccc cgccgtggcc agccacggcg gccgggccga
     1081 gttggtcgag gtcagcgagt ccggcgtggt ctcactccgc ctgagcggag gctgtcaggg
     1141 ctgtggcatg gccgcccaga ccctgcgcaa cggcatcgag gtggccctgc gggacaacat
     1201 ccccgaggtc accgagatcc tcgacgtcac cgaccacgcc gccggcgaaa acccctacgt
     1261 ccaagcctga gcgccaacca caccgaaaca acgcgccgag gtggtcgtga gaagggtcag
     1321 cggccacgtc accgccgacc cgaagccaaa ccctccacct caactgcagg tcgagatcga
     1381 gcacaagcct cagtgagcca cagacgcagg gacggggagg ggggccttcc tgagcgacgg
     1441 tttcgctcgt ttgcgtcagg agccagggag gcgcccctcc ccgtcccgca gcccgaagcg
     1501 gagcagctac ctcaaacgag ccgctcgaag atcatggcca tgccctgtcc ccctccgacg
     1561 cacatggtct ccaggccgaa ggtcttgtcg tgctcccgga ggttgtggat cagggtggcc
     1621 gtgatgcgag cgccggtcat gccgaacggg tgacccaggg cgatggcgcc gccggccacg
     1681 ttcagcttgt cccactcgat gcccagctcg tcggccgagg ggatgacctg ggcggcgaag
     1741 gcctcgttga tctcgaccag gtcgatgtcg ccgatgctca tcccggcccg ggccatggcc
     1801 tgcttggaag cctcgatggg accgaggccc atgatctcgg ggttcaaccc ggagacgccc
     1861 gaggccacga tccgggccag cggggtcaga ccgagggccc tggccttcgt gtcgctcatg
     1921 accagcacgg ccgcggcgcc gtcgttgagc gggcaggcgt tccccgccgt gatcgtgccg
     1981 tcgggccgga acacgggctt cagctcgctg agcttctcct tggtcgtgcc gtcccgggga
     2041 ccgtcgtcct tgctgaccac ggtgccgtcg gggagggtga tcggggtgat ctcccagtcg
     2101 aaccaaccct cctgctggga cttcaccgcc cgctgctggg acagggcggc gaactcgtcc
     2161 atccgttccc gggggacctg cttgtactcg gccacgttct cggccgtctc gcccatggcg
     2221 atgtagatgt cgggcaggcc ctcgggcggg gcccaggctc cggccccgcc ctgcgcccgc
     2281 tccgcggtgc gagcccgggc ggcgtcgaac ctcgggttgg tggagccggg catgccgtcg
     2341 gacatcccgc tcatgaagcg gctgaccgtc tcgacgccgg ccgagatgaa gcagtcgccc
     2401 tcgcccgcct tgatggcgtg ggcggccatt cgggtcgtct gcagcgacga cgagcagtag
     2461 cggttgacgg tgacgccggg gacgtcgggc atcccggact cgacggcgac cacgcggccg
     2521 atgttgtagc cggactcccc cgcgggctga ccgcagccca tgatcaggtc ctcgacctcg
     2581 gtgccgtcca cgccggaccg gctcaggagg gacgtgacga cctgggtggc caggtcgtcc
     2641 gggcgcatgt cgaccaggga tcccttgttg gcccggccga tggggctccg ggtggcggcg
     2701 acgacgacag cttcgggcat gagggttcct tcgttggacc ggatgcgagc gcaacgacgc
     2761 taccgcccgc cgtcagggcc accccagccg acatggggac ctgacggccg ggggagcgag
     2821 gcgtaggctc ggtcgtagtg cgtgtgcaga tcgggccggt ctcggccacg agcgtcacag
     2881 catgggtggg ctacgcccga aaggccctcg acgagatcgt cggcgcgccc ggaggtgacg
     2941 gcgtctggct gcccgacgac acggtcgcca ccttcgtcca ctacctcgac gagtgggaga
     3001 aggccgccgc cggtgtcgac gaggtgcgct ggaccgcaga gatccccgcc gatgaggccg
     3061 aatacctgtc ccatgccttc ttccgcatcg ccagcaacct ggccaaccgg gccgacgagc
     3121 ggggctacac cctggccccg cccgagagcg aggagttcta tcgtgccctc gtgacctcat
     3181 ttctgaccgc gctgaaagaa gagggccacg gcagcgatga gttctccgag cacctgcgct
     3241 cgttctggcc gggcctggag gaccagtcgt gaggctgctg ggtggcgtgc accacgtctc
     3301 gctgaacgtc gacgacgccg aggtcaccgg ccggttctac gaggacgtcc tcggcctgac
     3361 ccgcatcgaa cgcccggact tcggcatccc tggtgtgtgg ctgagcgccg gcgccgtgca
     3421 gatccacctg atcgagatcg aaggccacac cgccccggac ggccagcact tcgccttcca
     3481 ggtcgacgac gtggacgccg tccaggcccg actcgtcgaa cagggcgtcg acgccaccgc
     3541 gccgtcctcg tttcccggcg cgggccggca gtccttcttc cacgaccccg ccggcaacct
     3601 gatcgagctc aaccagcccg agtcgccggc gtgagcttcg gctcggcgcc gacccggggt
     3661 cgcctcctgg tagccgggcc gcccctggag gaccccaact tccgccggac cgtggtgttc
     3721 atgatcgagc acaacgaaga gggcgccctc ggcgtcgtgc tcaaccgccc ctctccgatc
     3781 gacctggccg acgctgtgcc ccagtgggcc gatctggcgg ccaacccgcc cgcggtcttc
     3841 atcggaggcc cggtcgagca gggcgccgtc ctcggcctcg gacgcctcgt cgccgaggac
     3901 cccccggagg ggctcacccc ggtccacgcc ggcatcggcg tgctcgacct cgaggccgat
     3961 cccgtccgtc tcgtgggcga tgtcggcggc gtgcgcctct tcacgggcta ctcgggctgg
     4021 ggccctggcc agttggagga cgagctggcg atgggcggct ggttcgtcgt ggacgccgag
     4081 ccggacgatg tgacgaccac cgagcccgac gacctctggc gggctgtcct gcgccgccag
     4141 cccgacgacg acctcgcccg cctcgccctc ttccccgacg acctctcggc caactgagga
     4201 tggcgacggc ggccctggat ggggtgagga tcgagttcga gctggccggg acgccggggc
     4261 aaccccggct gctgtccatc tccggctcgg gcagcgacct gcggcgagcg ccccggatcc
     4321 aggacgaagc ggtgctggcc gaacgcttcg agatcctggc ctacgaccac cggggactcg
     4381 gccgttccgt gcccgacgac ccccaccggg agccgaccat ggccgactac gcggcggacg
     4441 ccgtcggcct cctcgaccac gtgggctggg acacctgccg ggtcctcggg atctccttcg
     4501 gcggcatggt cgcccaggag ctggccgtca ccgcagcgca gcgcatcgag cgactggtgc
     4561 tggcctgcac ctcaccaggc ggagcgggag ggtcgtcctc gcccctccac acactgatgg
     4621 acatggccga cgacgagcgc ctggcgcagc aggtggcgtg gctcgccgac cgcacggtcg
     4681 acgaccccga gttgcgggcc ttctggaccg agatgatggc cggccttgcc gggagcgagc
     4741 cgaccgaggg cgagcgccat cagctgcagg cccggtccca tcacgacgtg tgggaccggc
     4801 tcccgcagct gga
//
LOCUS       DASTFV010000525         4125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1931213, whole genome shotgun sequence.
ACCESSION   DASTFV010000525 DASTFV010000000
VERSION     DASTFV010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1931213"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VFP02_09625"
     CDS             complement(<1..419)
                     /locus_tag="VFP02_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET9543331.1"
                     /translation="MKITIRTLAALVLALSLVAAGCGGDDSDDASSDTTEAGGGEAAA
                     CDPLPLKNDGTLTVATGEPAFPPWVSGVDADGNDDPTLGEGFESALVYAVAEQLGVAE
                     ADVEWVRTGFDEAIAPGEKDYDFNIQQFGITPERDEVV"
     gene            complement(449..1243)
                     /locus_tag="VFP02_09630"
     CDS             complement(449..1243)
                     /locus_tag="VFP02_09630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9543332.1"
                     /translation="MSPPALRVTEPAALADLYGRHPDVHPYGLADLDEPFWSRSTWYR
                     EGDAAVGVLDLGSGEPVLYAIAADADLAAATLTLLERLAPHLPDHFVITGPTGLADRL
                     APGFDPDWVIPHVKMVLPDGDRLPPDDPRVGWLDRGAADAVVALRETGGDASAFFVPA
                     LLDTGFYAGIEAGEDGALVAVAGVHVLSEARGVAAIGNVLTHPAHRRQGLARTLTATL
                     ARRLLARVPVVGLNVGTANTGARALYESLGFVPVVQYEEAELRRAH"
     gene            complement(1240..2094)
                     /locus_tag="VFP02_09635"
     CDS             complement(1240..2094)
                     /locus_tag="VFP02_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9543333.1"
                     /translation="MSAWTVGSATITTIVESDIPGIPPELFFPTGSAAEVSAQDWLVP
                     DYAAADGTITLRVQALVVTVAGRTVLVDPCVGDGKSRALPFWHQQAWGGMARFAEAGF
                     DAEAVDTVVHTHLHADHVGWDTHEEDGAWVPTFTNARHLYTEAEVAHWKADEQRGDED
                     VWADSVEPMFTAGLADLVAEDADLGDGLRLTPTTGHTPGHTSLWIESDGERAVITGDL
                     IHHPVQCAEPGWNEIGDVDTDQAAATRRAFLEAVADRDTLVIGTHFPTRPAGRVITVG
                     GAFRFLPG"
     gene            complement(2091..2441)
                     /locus_tag="VFP02_09640"
     CDS             complement(2091..2441)
                     /locus_tag="VFP02_09640"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9543334.1"
                     /translation="MSLAAWMDGYVKAWTSGQPEEIADLFTEDATYDPQTSEERWDGR
                     DAIIAGWQDVGDQPGTWTFEWQPMLETHGLSVITGRTEYFDEGRTFRNLWTIRLTDDG
                     RCREFTEWWIEEDW"
     gene            complement(2489..3178)
                     /locus_tag="VFP02_09645"
     CDS             complement(2489..3178)
                     /locus_tag="VFP02_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET9543335.1"
                     /translation="MTEVGTDGKAMVLTPLLPPAKPRLRGWFHQIAFLASIPLGLALV
                     AVADTTAAKVAALVYSLGASALYGTSAAYHRGHWSPRGRAWMKRLDHSMIFVLIAATY
                     TPICVLGLRGSGATLTLVGVWAGATLGVVLGMAGIAEKNGVGMTLYLIMGWVAALSAP
                     SLAQRLGPVNLGLIVIGGVAYTVGAVILGTNWPNPRPATFGYHEVWHTFTLVAGACFG
                     VVVWGLVLGAA"
     gene            complement(3186..>4125)
                     /locus_tag="VFP02_09650"
     CDS             complement(3186..>4125)
                     /locus_tag="VFP02_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9543336.1"
                     /translation="TGLKVSALSFGSWVTFGTQVDRDLALDLLQAARDGGVNFFDNAE
                     AYGGGESERLMGAAIAELGWERKTYVVSTKLFFGIDDDAVNLSDTLNRKYLTQAIEGS
                     LERFGLDFVDLLFCHRADPDTPIEETVWAMSDIVRSGQALYWGTSEWTADEIRAAHAV
                     AEKHHLHKPVVEQPQYNILETGRVEEEYAPLYDELGLGLTVWSPLASGLLTGKYLDGV
                     PEDSRAALEGYEWLRDMLTAEERMEKVRGLKAIADELDCSLAQMAIAWCTKNPRVSTV
                     ILGASRRSQLEENLAALEVADRLDDEVMARIRQAAR"
BASE COUNT          590 a         1371 c         1576 g          588 t
ORIGIN      
        1 acgacctcgt cgcgctccgg ggtgatgccg aactgctgga tgttgaagtc gtagtccttc
       61 tcgcccgggg cgatggcctc gtcgaagccg gtgcggaccc actcgacgtc ggcttcggcc
      121 acaccgagct gctcggccac ggcgtacacg agggcggact cgaagccctc accgagggtc
      181 gggtcgtcgt tgccgtcggc gtcgacgccg gagacccagg gcgggaaggc cggctcgccg
      241 gtggccaccg tgagggtccc gtcgttcttc aggggcagcg ggtcgcaggc cgccgcctca
      301 cctccgccgg cctccgtcgt gtcgctgctg gcgtcgtcgc tgtcgtcgcc gccgcaaccg
      361 gccgcgacca ggctgagggc cagcacgagg gcggccaggg tacggatcgt gatcttcacg
      421 gtgctctcct ccggatgaag gcgaaacctt agtgagcacg ccgtagctcg gcttcctcgt
      481 actggaccac cggcacaaag ccaaggctct cgtagagggc gcgggcaccg gtgttggccg
      541 tccccacgtt gaggccgacg accgggaccc tggccagcag gcggcgggcg agggtggcgg
      601 tcaaggtgcg ggccaggccc tggcgacggt gggccggatg ggtgaggacg ttcccgatgg
      661 ccgccacgcc gcgggcttcg gagagcacgt ggacgcccgc cacggcaacc agcgccccgt
      721 cctcaccggc ctcgatgccc gcgtagaagc ccgtgtccag gagggcgggc acgaagaagg
      781 cgctggcgtc gccgccggtc tcccgcaggg cgacgacggc gtcggcggcg ccgcgatcga
      841 gccagccgac ccggggatcg tccggcggga ggcggtcgcc gtcgggcagg accatcttca
      901 cgtgggggat cacccagtcg gggtcgaagc ccggggccag gcggtcggcc agccccgtcg
      961 gaccggtgat gacgaagtgg tcggggaggt ggggggccag gcgctcgagc agggtcaggg
     1021 tcgcggcggc gaggtcggcg tcggcggcga tggcgtagag gacgggctcg cccgagccca
     1081 ggtccaggac gcccacggcg gcgtcaccct cccggtacca ggtggaccgc gaccagaagg
     1141 gctcgtcgag gtcggccagg ccgtagggat gcacatccgg gtggcggccg tagagatcgg
     1201 ccagggcggc gggctcggtc acccgcaggg cgggcgggct cacccgggca ggaagcggaa
     1261 cgccccgccg acggtgatga cccgaccggc cggtcgggtg gggaagtggg tgccgatgac
     1321 gagggtgtcg cggtcggcca cggcctcgag gaaggcccga cgcgtcgcgg cggcctgatc
     1381 ggtgtcgacg tcgccgatct cgttccagcc cggctcggcg cactggaccg ggtggtggat
     1441 gaggtcgccg gtgatgacgg cccgttctcc gtcggactcg atccagagcg aggtgtgccc
     1501 gggcgtgtgg cccgtcgtgg gcgtgaggcg gaggccatcg ccgaggtcgg cgtcctcggc
     1561 cacgaggtcg gccaggccgg cggtgaacat gggctcgacg gaatcggccc agacgtcctc
     1621 gtcgccccgc tgctcgtccg ccttccagtg ggcgacctcg gcctcggtgt agaggtgacg
     1681 ggcgttggtg aacgtcggga cccaggcccc atcctcctcg tgggtgtccc aacccacgtg
     1741 gtcggcgtgg aggtgggtgt ggaccaccgt gtcgacggcc tcggcgtcga acccggcctc
     1801 cgcgaagcgg gccatcccac cccacgcctg ctggtgccag aacggcagcg cccgggactt
     1861 gccgtcgccc acgcacgggt cgacgaggac ggtgcggccg gcgaccgtca cgaccagggc
     1921 ctggacccgg agggtgatcg tcccgtcggc ggcggcgtag tcgggcacca gccagtcctg
     1981 ggcggacacc tcggcggcgg agccggtcgg gaagaagagc tcgggaggga tgccggggat
     2041 gtcggactcc acgatggtgg tgatcgtggc cgagccgacg gtccaggcgc tcaccagtcc
     2101 tcttcgatcc accattcggt gaactcgcgg cagcggccgt cgtcggtgag gcggatcgtc
     2161 cagaggttgc ggaaggtccg gccctcgtcg aagtactcgg tgcgccccgt gatgaccgac
     2221 aggccgtggg tctccagcat cggctgccat tcgaaggtcc aggtgccggg ttggtcgccc
     2281 acgtcctgcc agcccgcgat gatggcgtcc cgcccgtccc aacgttcctc gctggtctgc
     2341 gggtcgtagg tggcgtcctc agtgaacagg tcggcgatct cctccggctg gccggaggtc
     2401 cacgccttga cgtatccgtc catccacgcc gcgagactca tgcctcatgc tcccccgggg
     2461 ccgggagagc gtcaaacgag ccgcgggatc aggcggcgcc gagcacgagg ccccacacga
     2521 cgacgccgaa gcaggcgccg gcgaccaggg tgaaggtgtg ccagacctcg tggtagccga
     2581 aggtggcggg gcgggggttg ggccagttgg tgcccaggat gacggcgccc acggtgtagg
     2641 cgacgccccc gatcacgatc aggccgaggt tgaccgggcc gaggcgttgg gccaacgacg
     2701 gcgcggacag ggcggccacc caacccatga tcaggtagag ggtcatgccc acgccgttct
     2761 tctcggcgat gccggccatg cccagcacga cgccgagggt ggccccggcc cagacgccca
     2821 cgagggtgag ggtggcaccg ctgccgcgga ggccgagcac gcagatgggg gtgtaggtgg
     2881 cggcgatgag cacgaagatc atcgagtggt cgaggcgctt catccacgcc cggccccggg
     2941 gcgaccagtg gccgcggtgg taggcggcac tggtgccgta gagggcgctg gcccccagcg
     3001 agtacacgag ggcggcgacc ttggccgcgg tggtgtcggc cacggcgacg agggcgagcc
     3061 cgagggggat ggacgcgagg aaggcgatct ggtggaacca cccccgcagg cgaggcttgg
     3121 cgggcggcag gagcggcgtc agcaccatcg ccttcccgtc ggtccctact tcggtcatgt
     3181 gagcctcagc gagccgcttg tcggatgcgg gccatcacct cgtcgtccag gcggtcggcg
     3241 acctccaggg cggccaggtt ctcctcgagc tgggagcggc ggctcgcccc gaggataacc
     3301 gtcgagaccc gggggttctt cgtgcaccag gcgatcgcca tctgggccag cgagcagtcc
     3361 agctcgtcgg cgatggcctt gaggccccgg accttctcca tccgctcctc ggccgtgagc
     3421 atgtcccgca accactcgta gccctccagg gcggcgcggc tgtcctcggg tacgccgtcg
     3481 aggtacttgc cggtgaggag gcccgacgcc agcgggctcc acacggtcag gccgaggccg
     3541 agttcgtcgt acaggggggc gtactcctcc tcgacccgcc cggtctcgag gatgttgtac
     3601 tgcggctgct cgaccaccgg cttgtgcagg tggtgcttct cggccacggc gtgcgcagcc
     3661 cggatctcgt ccgcggtcca ctccgacgtg ccccagtaga gggcctgacc cgagcggacg
     3721 atgtcggaca tggcccagac ggtctcctcg atcggtgtgt cgggatccgc ccggtggcag
     3781 aagaggaggt ccacgaagtc gaggccgaag cgctcgaggg agccctctat ggcctgggtc
     3841 aggtacttgc ggttgagcgt gtcggagagg ttgacggcgt cgtcgtcgat gccgaagaag
     3901 agcttggtgc tcaccacgta ggtcttgcgc tcccagccca gctcggcgat ggcggcgccc
     3961 atgaggcgct cggactcacc cccgccgtag gcctcggcgt tgtcgaagaa gttgacgccg
     4021 ccgtcgcgag ccgcctggag gaggtcgagg gcgagatccc ggtcgacctg ggtgccgaag
     4081 gtgacccagg agccgaagga gagcgcgctc accttcagcc cggtg
//
LOCUS       DASTFV010000526         2225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1936111, whole genome shotgun sequence.
ACCESSION   DASTFV010000526 DASTFV010000000
VERSION     DASTFV010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936111"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="VFP02_09655"
     CDS             complement(<1..1084)
                     /locus_tag="VFP02_09655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HET9543337.1"
                     /translation="MYQTLKSVIRFKPLEFDPVERRLAKAADVDDLRRIAQRRLPQGV
                     FDYIDGGAEDERTYGANTEAYRRRTFRPRVLKDVSNVDPATTLLGRRIPLPLVLAPTG
                     FARIADPEGELAVARAAAKVGVPYTLSTIGTRSIEEIAAVNPGPKWFQLYVFRDRGLV
                     KELVERTKAAGYEAITLTVDTAVLGRRERDIRRGFTLPPKIGLGTIVDGALHPGWTWA
                     FVRAEPIVFANVAGKDVGDGSDAVSLADYINGQFDPSLSWPDVEWLRSIWDGPLVIKG
                     IQSVADAEIAADVGIEAVALSNHGGRQLDTAPATLDLVEPVVQAVGDRLEVICDGGVR
                     RGSDVVKAVALGAKAVMAGRVHFYALG"
     gene            1147..>2225
                     /locus_tag="VFP02_09660"
     CDS             1147..>2225
                     /locus_tag="VFP02_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9543338.1"
                     /translation="MHDGVRPTIDLCDPEFYRSAGMHEAFTWMRANEPVYRDEANGLW
                     AVTRHADLLDVERRSDVFVSGRGYRRHWEPSENNMIASDDPVHQQQRRLVSRGFTPKA
                     VRRHEDGLAELIAELLDPIDGEVEVVDALAAQLPARMTARLLGFPEENWRAVKSWSER
                     LMRLDAQFEDPAIEMAVGLACMEFNQQLMEMVPARRECPVDDLVSAWANAYEHDKVFN
                     ETGLFIAGGAETTRTAIAHGLRAFADHPDQWDRLATEPDLVPTAVDEVIRWVTPLNNF
                     FRTAVEDSTIGAQDVADGDRVILLYPSANRDEAVFEDPFTFDTTRRPNPHVAFGYGTH
                     FCIGANFAKYELVMLFAELTRRFTAP"
BASE COUNT          332 a          799 c          781 g          313 t
ORIGIN      
        1 cgcccaacgc gtagaagtgg acccggccgg ccatgaccgc cttggccccg agggccacgg
       61 ccttgacgac gtcgctaccg cgccggaccc caccgtcgca gatgacctcg agccggtcgc
      121 ccacggcctg gacgacgggt tcgaccaggt ccagggtggc gggggcggtg tccagctgcc
      181 ggccgccgtg gttcgagagg gcaacggcct cgatgcctac gtcggccgcg atctcggcgt
      241 cggccacgct ctggatgccc ttgatgacca gggggccgtc ccagatcgag cgcagccatt
      301 cgacgtcggg ccaggacagc gacgggtcga actggccgtt gatgtagtcg gccagcgaga
      361 cggcgtcgga cccgtcgccc acgtccttgc cggccacgtt ggcgaagacg atcggctcgg
      421 cccggacgaa ggcccacgtc caccccgggt ggagcgcacc gtcgacgatc gtgcccaggc
      481 cgatcttggg gggcagcgtg aagccccggc ggatgtcccg ctcccgccga ccgaggacgg
      541 cggtgtcgac ggtcagggtg atggcctcgt accccgccgc cttcgtgcgc tcgaccagct
      601 ccttgaccag cccccggtcc cggaagacgt agagctggaa ccacttgggg ccggggttga
      661 cggccgcgat ctcctcgatg gagcgggtgc cgatggtgga cagcgtgtag gggaccccga
      721 ccttggccgc agcccgggcc accgccagct cgccctcggg gtcggcgatg cgggcgaagc
      781 cggtgggggc gaggacgagg gggagcggga tccgccggcc gagcagcgtg gtggccgggt
      841 ccacgttgct gacgtccttc agcacccggg ggcggaacgt gcgccggcgg taggcctcgg
      901 tgttggcgcc gtaggtgcgc tcgtcctcgg cccccccgtc gatgtagtcg aagaccccct
      961 ggggcagccg ccgctgggcg atccgacgca ggtcgtcgac gtcagcggcc ttggccagcc
     1021 gccgctcgac gggatcgaac tcgagcggct tgaaccggat gaccgacttg agcgtctggt
     1081 acatgcccac tgcgcccgag ggtacccacg cctcggcggg cggtccgaac cggccagact
     1141 gtgacgatgc acgacggagt gcgccccacc atcgacctgt gtgatcccga gttctaccgc
     1201 tcggcgggga tgcacgaggc cttcacctgg atgcgggcga acgagcccgt gtaccgcgat
     1261 gaggccaacg ggctgtgggc ggtgacccgg cacgccgacc tgctcgacgt cgagcggcgc
     1321 tcggacgtgt tcgtctcggg ccggggttac cgccggcact gggagccgtc ggagaacaac
     1381 atgatcgcct ccgacgaccc cgtccaccag cagcagcggc gactggtgtc ccgggggttc
     1441 acgcccaagg ccgtccgtcg ccacgaggac gggctggccg agctcatcgc cgagctcctc
     1501 gatccgatcg acggcgaggt cgaggtcgtc gacgcgctgg cggcccagct cccggcccgg
     1561 atgacggccc ggctcctcgg cttccctgag gagaactggc gggcggtcaa gagctggtcg
     1621 gagcgcctca tgcgcctcga cgcccaattc gaggacccgg cgatcgagat ggccgtcggc
     1681 ctggcctgca tggagttcaa ccagcagctc atggagatgg tcccggcccg acgggagtgc
     1741 ccggtggacg acctcgtgtc ggcgtgggcc aacgcctacg agcacgacaa ggtcttcaac
     1801 gagaccggcc tgttcatcgc cggcggggcc gagacgaccc ggaccgccat cgcccacggc
     1861 ctgcgggcct tcgccgacca ccccgaccag tgggaccgac tggccacgga gcccgacctc
     1921 gtgcccaccg cggtggacga ggtcatccgc tgggtcacgc cgctcaacaa cttcttccgc
     1981 accgccgtcg aggactcgac gatcggagcg caggacgtgg ccgacggcga ccgggtgatc
     2041 ctgctctacc cctcggccaa ccgggacgag gccgtgttcg aggacccctt caccttcgac
     2101 accacccgca ggcccaaccc ccacgtggcc ttcggctacg gcacccactt ctgcatcggg
     2161 gccaacttcg ccaagtacga gctggtcatg ctcttcgccg agctgactcg ccggttcacc
     2221 gcccc
//
LOCUS       DASTFV010000527         3569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1939992, whole genome shotgun sequence.
ACCESSION   DASTFV010000527 DASTFV010000000
VERSION     DASTFV010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1939992"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VFP02_09665"
     CDS             complement(<1..498)
                     /locus_tag="VFP02_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HET9543339.1"
                     /translation="MPYYRSVGEVPRKRHTVAPSDEGRRSEELMGQEGFAEESSLLYH
                     RHSPSALSAVEVVDEPSGADFSADLPLTPRHIRTGGLPAGRDVVFGRQPVLGNPDVVL
                     CWAAATEDSDLYRNAIGDELVYIHDGEATLETSFGALPVTSGDYVVIPRGTTHRWVLG
                     GDRLDV"
     gene            complement(579..1811)
                     /gene="fahA"
                     /locus_tag="VFP02_09670"
     CDS             complement(579..1811)
                     /gene="fahA"
                     /locus_tag="VFP02_09670"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HET9543340.1"
                     /translation="MAIDVSIGVPVPDGSPFTLANLPFGVANGRVHIAIGDHAVDVAA
                     AAGFGLLDVDPTVVEGCRLDGLLAAGPTTWRGLREEVGQVLADEPGRLAKTMVDRATL
                     AMELPVTVGDYVDGYAGLHHATNLGRILRPDGEPLQPNWRQLPVAYHGRSGTIVVGGG
                     EIGRPSGQVLVDGTPVLAPSAQLDIELELGAIVGVANAPGEPIPVGEADTHLFGFVLV
                     NDWSARDIQAWEYQPLGPFLGKSFATSVSPWVVTVDALAPHRVVGLAGEQDPPPLSYL
                     RGDPAVPDLHLTVDLTSAQMRDEGGEPFRVGEVDLAPSLYWTPAQQLAHCTVNGATVR
                     PGDLLASGTISGPDHTTQAGSLIERTWRGAEPFTLPTGEERSFLADGDQVVLDGWAGD
                     GADRVGFGPLSGTIAAAR"
     gene            complement(1816..2151)
                     /locus_tag="VFP02_09675"
     CDS             complement(1816..2151)
                     /locus_tag="VFP02_09675"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HET9543341.1"
                     /translation="MAYDEALADRIRAVLADAPGVDERKMFGGIAFLLDGNMAVGIVG
                     EELMVRVGPDAWADALAQPYAREMDFTGRSMKGFVYVSGDGVAEDSDLGGWVAQGTDF
                     AGSLPPKQR"
     gene            complement(2153..2650)
                     /locus_tag="VFP02_09680"
     CDS             complement(2153..2650)
                     /locus_tag="VFP02_09680"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9543342.1"
                     /translation="MGGPADPRLRLRVPAYGGGIAIDVETETVIARPVAEVSAYAADP
                     TNAPEWYDNIESAAWETDPPLRVGSRVAFVARFMGRQLTHTYEIVELVPEERLVMRTA
                     EGPFPMETTYTWVSTAEGYTRMTLRNWGEPTGFFKLVAPIMVGAMRRANNKDLDKLKT
                     ILQGV"
     gene            complement(2617..2985)
                     /locus_tag="VFP02_09685"
     CDS             complement(2617..2985)
                     /locus_tag="VFP02_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9543343.1"
                     /translation="MDFRLELVAIPVTDVDRAKAFYEQVGFTVDHDHVVSDEMRFVQV
                     TPPGSACSIAFGLGVSEAEPGSVQGLQLVVDDIEAAKAELEGRGIEVKGIEDFDWGRF
                     VWFADPDGNGWAVQQIPDYG"
     gene            3120..>3569
                     /locus_tag="VFP02_09690"
     CDS             3120..>3569
                     /locus_tag="VFP02_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET9543344.1"
                     /translation="MKLATIRDGDRTKAVRIDGDEAVELDAADVGEVLRRDDWAAWAG
                     AGEGARRPVAELDYAPLIVAPDKIVCCGLNYRTHILEFTRELPSYPTLFAKYRSTLTG
                     ANDDIELPTVTEAVDWEVELAVIIGMQGRDIAGADAAAHIAGYAVLND"
BASE COUNT          540 a         1282 c         1238 g          509 t
ORIGIN      
        1 cacgtcgagc cggtccccac cgaggaccca acggtgcgtg gtgccgcggg ggatgactac
       61 gtagtccccg ctcgtaaccg gcagagcgcc gaagctggtc tccagcgtgg cctcgccatc
      121 gtggatgtag acgagctcgt cgccgatggc gttccggtag aggtcgctgt cctcggtggc
      181 cgcggcccag cagaggacga cgtccgggtt gccgaggacg ggctgacgac cgaacaccac
      241 gtcgcggcct gccggcagcc caccggtgcg gatgtggcgc ggggtcagcg gcaggtcggc
      301 ggagaagtcg gcgccggagg gctcgtccac cacctcgacg gcactcaggg cgctgggcga
      361 atgccgatgg tagagcaggg aagactcctc ggcgaagccc tcctggccca tcagctcctc
      421 actccgtcgc ccctcgtcgg acggggcgac cgtgtggcgc ttgcggggca cctcaccgac
      481 cgaccggtag tacggcatgg cggtttccta ccccaaccct ctcgttctgg cccctgattc
      541 ggctcccaga ggagctgaat caggggccag aaggagcgtc atcgggcggc ggcgatcgtg
      601 ccggacaggg ggccgaagcc gacgcggtcg gcgccgtcac cggcccagcc gtcgaggacg
      661 acctggtcgc cgtcggccag gaacgagcgc tcctcccccg tcgggagcgt gaatggctcg
      721 gccccccgcc aggtccgctc gatgagcgag ccggcctgcg tcgtgtggtc tgggccggag
      781 atggtgccgc tggcgagaag gtcgccgggg cgtacggtgg ccccgttgac cgtgcagtga
      841 gccagttgct gggccggcgt ccagtagagg gaaggggcca ggtcgacctc gcccacccgg
      901 aacggctcgc ccccctcgtc ccgcatctga gcgctggtca ggtcgacggt caggtgcagg
      961 tcgggcacgg cgggatcgcc ccggaggtac gacagcggcg gggggtcctg ctcgccggcc
     1021 agcccgacga cccggtgggg tgccaatgca tccacggtca cgacccatgg tgacaccgac
     1081 gtggcgaacg acttgcccag gaacggaccc agcggctggt actcccacgc ctggatgtcc
     1141 cgcgccgacc agtcgttgac gaggacgaag ccgaagaggt gcgtgtcggc ctcgcccacc
     1201 gggatgggct cgcccggcgc gttggccaca ccgacgatcg cgcccagctc cagctcgatg
     1261 tcgagctggg ccgagggggc caagaccggg gtgccgtcga cgagcacctg cccggacggg
     1321 cgcccgatct cgccgccacc gacgacgatc gtccccgacc gcccgtggta agcgaccggg
     1381 agctgacgcc agttcggctg caggggctcg ccgtccggcc gcaggatgcg accgaggttg
     1441 gtggcgtggt ggagcccggc gtagccgtcg acgtagtcac cgacggtgac cggcagctcc
     1501 atggccagcg tcgcccggtc aaccatcgtc ttggccagac gcccgggctc gtcggccagc
     1561 acctggccga cttcctcccg caggcctcgc caggtcgtcg gcccggcggc cagcaggccg
     1621 tccaaccggc agccctcgac gactgtcggg tcgacgtcga gcagcccgaa gccggccgcg
     1681 gccgccacgt cgacggcgtg gtcgccgatg gcgatgtgga cgcggccgtt cgccacgccg
     1741 aacggcaggt tggccagggt gaacggcgag ccgtcgggca ccgggacacc gatgctcacg
     1801 tcgatggcca cgggctcagc gttgcttcgg cgggagggag ccggcgaaat ccgtcccttg
     1861 cgcgacccag ccgccgagat cggagtcttc ggccacgccg tcaccactca cgtagacgaa
     1921 gcccttcatc gaccggccgg tgaaatccat ctcccgggcg tagggctggg cgagggcgtc
     1981 ggcccaggcg tcggggccga ctcgaaccat gagctcctcc cccacgatcc ccaccgccat
     2041 gttgccgtcg agcaagaacg cgatgccgcc gaacatcttg cgctcgtcga cgccgggggc
     2101 gtcggccagc accgcccgaa tccgatcggc cagcgcctcg tcgtaggcca cctcagaccc
     2161 cctgcaggat cgtcttcagc ttgtccaggt ccttgttgtt cgctcgccgc atcgcgccga
     2221 ccatgatcgg cgcaaccagc ttgaagaagc cggtgggctc gccccaattg cgcagcgtca
     2281 tgcgggtgta cccctcggcg gtggacaccc acgtgtaggt cgtctccatc gggaacggcc
     2341 cctcggcggt gcgcatgacc agccgctcct ccggcaccag ctccacgatc tcgtaggtgt
     2401 gggtcagctg ccggcccatg aagcgggcga cgaaggccac ccgggagccg acccgcaacg
     2461 gcgggtcggt ctcccatgcg gccgactcga tgttgtcgta ccactcgggg gcgttcgtcg
     2521 ggtcggccgc gtaggccgac acctcggcca cgggtcgggc gatgaccgtc tcggtctcga
     2581 cgtcgatggc gatgccccca ccgtacgccg ggaccctcag ccgtagtcgg ggatctgctg
     2641 gaccgcccag ccgttgccgt cagggtcggc gaaccagacg aagcggcccc aatcgaagtc
     2701 ctcgatgccc ttgacctcga tgccgcggcc ctcgagctcg gccttggccg cctcgatgtc
     2761 gtcgacgacc agctggaggc cctggaccga gcccggctcg gcctcgctga cgccgaggcc
     2821 gaaggcgatc gagcaggccg agcccggcgg cgtcacctgc acgaagcgca tctcgtcgct
     2881 cacgacgtgg tcatggtcga cggtgaagcc gacctgctcg tagaacgcct tggcccggtc
     2941 cacgtcggtc accgggatcg ccaccagctc cagtcggaag tccatcctct ggctcctctc
     3001 gtcgtcgtcc tcgggcccgt ccatcgagcc ccaggagagc gtctcatccc ttgcggacag
     3061 atcctggccg caagaccaga tcctcaggga ggatccggtg gggtcatact ggtcgaccga
     3121 tgaagctggc gacgatccgc gacggtgatc gcaccaaggc cgtgcgcatc gacggtgacg
     3181 aggccgtcga gctcgacgcc gccgacgtgg gcgaggtcct gcgccgggac gactgggcgg
     3241 cctgggcggg ggcgggggaa ggtgcccgcc gacccgtcgc cgagctggac tacgcgccgc
     3301 tgatcgtcgc ccccgacaag atcgtctgct gcggcctcaa ctaccgcacg cacatcctcg
     3361 agttcacacg cgagttgccg tcctacccga cgctcttcgc caagtaccgc tcgaccctga
     3421 ccggggccaa cgacgacatc gagttgccga ccgtcaccga agccgtcgac tgggaggtcg
     3481 agctggcggt catcatcggc atgcagggcc gcgacatcgc cggcgccgac gccgccgccc
     3541 acatcgccgg gtacgccgtg ctcaacgac
//
LOCUS       DASTFV010000528         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1944794, whole genome shotgun sequence.
ACCESSION   DASTFV010000528 DASTFV010000000
VERSION     DASTFV010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944794"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="VFP02_09695"
     CDS             <1..953
                     /locus_tag="VFP02_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HET9543345.1"
                     /translation="WVTIATERAARPSDFAPRFLPVEAEPDRRCPFCPGHEDDFPPAL
                     ESYARDGEWLVRVVPNKYPAFRGVEPLDLTVLGPVFTQAPASGIHEVLVLSPDHQRAW
                     ADLDDRQVALVMAAIRDRVEDHGRHAVVRHSLVIVNHGREAGASLEHPHGQLLGTPFV
                     SRVVGDEATAFADFDDKCLLCSTVDEERREGLRVVDDHGAAVVLSPYWAGSPYELLVV
                     PTTHQGDVTRSDPPDVVGVGRAVRDGLRRVRALLGDVPYNLVLHTAPRGDDGPYHWHV
                     HVVPRVTSVAGFEQGTGALINIVAPELAAQQLVRQDPGGA"
     gene            complement(950..1141)
                     /locus_tag="VFP02_09700"
     CDS             complement(950..1141)
                     /locus_tag="VFP02_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543346.1"
                     /translation="MRCAECDHGFECCHGTSILHADGTAECLGDGACTLAHGLHPWAT
                     TCDEAGCTCRVDQDLPVAA"
     gene            complement(1198..1455)
                     /locus_tag="VFP02_09705"
     CDS             complement(1198..1455)
                     /locus_tag="VFP02_09705"
                     /inference="COORDINATES: protein motif:HMM:NF020295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HET9543347.1"
                     /translation="MASADPSPTPNPNAMKFTLDVTLDEMVDVRSADAAEGNPLAERI
                     FAIPGVVMLFGTADFLTVSREEGADWDTIVPAVVAVIAEEL"
     gene            1595..2035
                     /locus_tag="VFP02_09710"
     CDS             1595..2035
                     /locus_tag="VFP02_09710"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9543348.1"
                     /translation="MPRIEVATTIDAPPDAVWARIEDVASHVDWMADAEAIRFTSDQT
                     AGVGTTFDCDTRVGPLKLTDRMTVTEWEPDRAMGVRHEGLVTGDGRFTLTAVGEARTD
                     FAWAETLHFPWWMGGPVGGAAGGPVLRAVWRQNLARLKVLVEQS"
     gene            complement(2032..>2251)
                     /locus_tag="VFP02_09715"
     CDS             complement(2032..>2251)
                     /locus_tag="VFP02_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543349.1"
                     /translation="NLIFTFAIPGISVGGHIGGLIGGALAGWLLILLPLRSRALPSFL
                     PTAAVLALGVAVIGASMWAASQWMDPVF"
BASE COUNT          322 a          819 c          804 g          306 t
ORIGIN      
        1 agtgggtcac gatcgccacc gagcgggccg cccgcccgtc ggacttcgcc ccccgcttcc
       61 tgcccgtcga ggccgagccc gaccgccgct gcccgttctg cccgggccac gaggacgact
      121 tcccgcccgc cctcgagtcc tacgcccggg acggtgagtg gctggtccgc gtcgtcccca
      181 acaagtaccc ggccttccgg ggggtggagc ccctggacct gacggtgctc gggccggtgt
      241 tcacccaggc tccagccagc ggcatccacg aggtgctcgt gctctccccc gatcaccagc
      301 gggcgtgggc cgacctggac gaccgccagg tcgccctggt catggccgcc atccgtgacc
      361 gggtggagga ccacggccgc cacgccgtcg tccgccattc gctggtgatc gtcaatcacg
      421 gccgggaggc gggggcctca ctcgagcatc cccacggcca gctcctcggc acgccgttcg
      481 tgtcccgcgt ggtgggcgac gaggccacgg ccttcgccga cttcgacgac aagtgcctgc
      541 tgtgctcgac cgtcgacgag gagcggcgcg aagggctccg cgtggtggac gaccacggcg
      601 cggctgtggt cctcagcccc tactgggcgg gctcgcccta cgagctcctg gtcgtgccca
      661 cgacccacca gggcgacgtc acccgctcgg acccgccgga cgtggtgggc gtcggacggg
      721 cggtgcggga cgggctgcgc cgggtccggg ccctgctcgg cgatgtcccc tacaacctcg
      781 tcctgcacac ggctccccgg ggcgacgacg ggccctacca ctggcacgtc cacgtggtcc
      841 cccgcgtcac cagcgtggcc ggcttcgagc agggcaccgg ggccctgatc aacatcgtgg
      901 ctccggagct ggccgcccag cagctggtcc gccaggaccc gggtggggcc taggcggcga
      961 cggggaggtc ctggtcgacg cggcaggtgc agcccgcctc gtcgcaggtc gtggcccagg
     1021 ggtgcaggcc gtgggccagc gtgcaggccc cgtcacccag gcactccgct gtgccgtcgg
     1081 catggaggat cgaggtgccg tggcagcact cgaacccgtg gtcacactcg gcgcatcgca
     1141 tggggagagt ctgcgcaggg ggtgtgacag gaatcggtga tcccggtcgt cccgggctca
     1201 gagctcctcg gcgatcacgg ccacgacagc ggggacgatg gtgtcccagt ccgccccttc
     1261 ttcgcggctg acggtgagga agtcggccgt gccgaacagc atcaccacgc cggggatggc
     1321 gaagatgcgc tcggccagcg ggttcccctc cgccgcgtcc gcgctgcgta catcgaccat
     1381 ctcgtccagg gtcacgtcca gggtgaactt catggcgttg gggttcggcg tgggtgacgg
     1441 atcggcggag gccatggccc gaccatagga gccgagccgc caggcgaggc gaccggcaag
     1501 cacagcctcg ctactcgccg agcgcagcga ggcgagccac tcgttcaccg ggcgacggcc
     1561 gaacccatgc gatcgagtct ttaggctcga cgcgatgccc aggatcgagg tggccacgac
     1621 catcgacgcg ccgcccgatg ccgtgtgggc ccgcatcgag gatgtcgcct cccacgtgga
     1681 ctggatggcc gacgccgagg ccatccgctt cacctccgac cagaccgcgg gcgtgggcac
     1741 cacgttcgac tgcgacacca gggtcggccc cctgaagctg accgaccgga tgacggtcac
     1801 ggagtgggaa ccggatcgcg ccatgggagt ccgtcacgag ggcctcgtca ccggcgatgg
     1861 tcgcttcacg ctcaccgcgg tgggcgaggc ccggaccgac ttcgcctggg ccgagaccct
     1921 gcacttcccc tggtggatgg gcggccccgt cggcggtgcc gccggcggcc ccgtcctccg
     1981 ggccgtctgg cgccagaacc tcgcccgcct caaggtcctc gtcgaacagt cctagaaaac
     2041 cgggtccatc cactgggacg cggcccacat cgaggcgccg atgacggcca cccccagggc
     2101 caggaccgcg gcggtgggga ggaacgaggg caaggcccgg gaccggagcg gcaggaggat
     2161 cagcagccat ccggccaggg cgccgccgat cagcccgccg atgtggccgc cgaccgagat
     2221 gccggggatg gcgaaggtga agatcaggtt g
//
LOCUS       DASTFV010000529         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1954166, whole genome shotgun sequence.
ACCESSION   DASTFV010000529 DASTFV010000000
VERSION     DASTFV010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1954166"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VFP02_09720"
     CDS             complement(<1..659)
                     /locus_tag="VFP02_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543350.1"
                     /translation="MWAAVLLPPYFRDRNDTRAAGKHSPVSNKLGALSRSFGNRERSR
                     YLPVASSSLGAPQKAGSPLPAGSRHPLTRRGSPQLVPSAPEPQSSAFSILGPPGSSPE
                     AEEFAVEDARFLLAPEVDETTPEATRTRRAIPKDVLASRAARKRRREVLYTLLAASGL
                     SLLMAIGLGGPVLVLHVLIDIALVVYVGLLVRIQRAKAEREIQVAFLPHGGDGVAPSE
                     LL"
     gene            complement(840..1346)
                     /gene="moaC"
                     /locus_tag="VFP02_09725"
     CDS             complement(840..1346)
                     /gene="moaC"
                     /locus_tag="VFP02_09725"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HET9543351.1"
                     /translation="MSDVGLTHLDPLGRARMVDVTPKEATHRRAIARGRVFMKPETTS
                     LVARGAITKGDVLAVARVSGIQAAKRTADLIPLCHPLLVGSVLVNFRIEDEYVEIEAQ
                     VETVDRTGVEMEALTACSVAALTIYDMCKSADRSMVIGDVSLWEKTGGRSGTYRRAET
                     EDAPVADS"
     gene            complement(1391..2374)
                     /gene="moaA"
                     /locus_tag="VFP02_09730"
     CDS             complement(1391..2374)
                     /gene="moaA"
                     /locus_tag="VFP02_09730"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HET9543352.1"
                     /translation="MAQPLVDSFGRVHRDLRISVTDRCNFRCVYCMPEEGMDWTPRDE
                     ILTFEEIERIARVLVDRFSFDSIRLTGGEPTVRARLPVLVQKLSALGVDLAMTTNGAT
                     LGLLADDLVAAGLNRINISLDSLRADRFLQLTRRDALDQVLDGIDAALAAGFTPVKVN
                     VVLMRGINDDEIVDFATFGRERGVQVRFIEFMPLDADGAWRAEQVISRDEIVAAIGAV
                     YPLEAITRDHHPAELYRYSDGAGEIGIIPTVTDAFCGSCDRVRLTAEGGLRNCLFALE
                     EEDLRGLLRGGGTDDDLAAAVVANIGAKWAGHQINQVHFIRPARSMSQIGG"
BASE COUNT          387 a          839 c          961 g          364 t
ORIGIN      
        1 aggagctcgg agggggccac cccgtcgccg ccgtggggca ggaaggcgac ctggatctcg
       61 cgctcggcct tggcccgctg gatgcggacg aggagaccga cgtagacgac cagggcgatg
      121 tcgatcagca cgtggagaac gaggaccggc ccgcccaggc cgatcgccat gagcaggctg
      181 aggccgctgg cggcgagcag ggtgtacagc acctcgcggc ggcgcttgcg ggcggcgcgg
      241 gaagccagga cgtccttcgg gatggcccga cgggtcctgg tcgcctccgg cgtcgtctcg
      301 tcgacctccg gggcgagcag gaagcgggcg tcctcgaccg cgaactcctc ggcctcgggg
      361 gaggatcccg gggggccgag gatcgagaag gcggaggact ggggctccgg ggcgctgggg
      421 acgagctggg gggaaccccg gcgggtcagc gggtgccgag agccggcggg cagggggctg
      481 ccggccttct ggggggcacc gagggacgac gacgccaccg gcaggtagcg cgagcgctcc
      541 cggttaccga acgagcggga aagggcaccg agcttgttgg agaccggact gtgcttgccg
      601 gcggcccggg tgtcgttgcg atctcggaag taggggggca gcagtacggc ggcccacata
      661 ccaacgagga tcagcaggac tatgggtgtc actcagaggg ttcctctgtc ggcggcaggg
      721 tctcgcccgt gtcgggcggt ggttcgggct cttggggctc cgttacgaac attacgtttc
      781 tactacgaaa ccgcaaccct agcgaatcgg gggcgggtcc gtggccatcg cgaaatgact
      841 caggagtcag ccactggagc gtcctcggtc tcggcccgcc ggtaggtccc ggaacggccg
      901 ccggtcttct cccagaggct gacgtcgccg atgaccatcg agcggtcggc ggacttgcac
      961 atgtcgtaga tggtgagggc ggcgaccgaa caggcggtca gggcctccat ctcgacgccc
     1021 gtgcggtcga cggtctcgac ctgggcctcg atctcgacgt actcgtcctc gatgcggaag
     1081 ttgacgagga ccgagcccac gagcagtggg tggcagagcg ggatgaggtc ggcggtgcgc
     1141 ttcgccgcct ggatgcccga cacccgggcc acggccagga cgtcaccttt ggtgatcgct
     1201 ccccgggcga ccagggaggt cgtctccggc ttcatgaaga cccgtccccg ggcgatggcc
     1261 cggcggtgcg tggcctcctt gggcgtcacg tcgaccatgc gggcgcgccc gagggggtcg
     1321 aggtgggtca ggccgacgtc gctcatgggc ccgagcctag tgagcggggc ccgttcggcc
     1381 gaaggggacc tcagccgccg atctggctca tcgaccgggc cggtcggatg aagtgcacct
     1441 gattgatctg gtggcccgcc catttggccc cgatgttggc caccaccgcg gcggccaggt
     1501 cgtcgtcggt gccgccgccc cgcagcaagc cccgcaggtc ctcctcctcg agggcgaaca
     1561 ggcagttgcg caagccgccc tcggccgtga gccgcacccg gtcacaggag ccgcagaagg
     1621 cgtcggtgac cgtcgggatg atgccgatct cgccggcacc gtccgagtag cggtagagct
     1681 cggcggggtg atggtcccgg gtgatcgcct cgagcgggta cacggccccg atggcggcca
     1741 cgatctcgtc ccggctgatc acctgctcgg cccgccacgc gccatcggcg tcgaggggca
     1801 tgaactcgat gaagcggacc tgcacgcccc gctcgcgccc gaaggtggcg aagtcgacga
     1861 tctcgtcgtc gttgatgccc cgcatcagca cgacgttgac cttcaccggc gtgaacccgg
     1921 cggccaaggc ggcatcgatg ccgtcgagca cctggtccag cgcgtcgcgg cgggtcagct
     1981 gcaggaagcg gtcggcccgc agcgagtcga gggagatgtt gatgcggttg agcccggcgg
     2041 cgaccaggtc gtcggccagc agcccgagcg tggcgccatt ggtcgtcatg gccaggtcga
     2101 cgccgagggc ggacaacttc tggaccagga cgggcaggcg ggcgcgcacg gtgggctcgc
     2161 caccggtcag gcggatcgag tcgaaggaga agcggtcgac cagcacccgg gcgatgcgct
     2221 cgatctcctc gaaggtgagg atctcgtccc ggggcgtcca gtccatgccc tcctcgggca
     2281 tgcagtagac gcagcggaag ttgcagcggt cggtgaccga gatgcgcagg tcgcgatgca
     2341 cccgaccgaa cgagtcgacc agcggctgcg ccatggcctc caccctacgg gggcgccacc
     2401 ccgacccgca tccgatactc agccggcggg cacctcgtcg agcgggatga cgctcaacgt
     2461 ctcgccttcg gccacgccgt cgccgtcctc caccaccgcc agggcgttgg ctgaggccat
     2521 ggcggcgagg tggtggctgc cctggccccc g
//
LOCUS       DASTFV010000530         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1956825, whole genome shotgun sequence.
ACCESSION   DASTFV010000530 DASTFV010000000
VERSION     DASTFV010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956825"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="VFP02_09735"
     CDS             complement(<1..1155)
                     /locus_tag="VFP02_09735"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9543353.1"
                     /translation="MTDLFDRFAVIDVDTHLTEPPDLWTSRVPAAMQEHVPRIVRRGD
                     LEMWVAGDEFIGAPGAYSMAGYDGVIPDMPKDFTEIPPAAYDAEARLAFLDREGIQAQ
                     VLYPNVGGFGNGFFLRLGDREVVAASVRAYNDFLTEWCSAAPDRLLPVIATPFWDLEF
                     AVTEIERAAAMGHRAVNMCSQPQNYDEPPLGSTHWDPIWAAARDTGLSVSFHIGGGGL
                     GNLMDDIGGIGLQANLGKESAMLILDNMRCLGDLFFGGVCHRFPEVRFVSVESGVGWL
                     PGVLEAFDWQWRNGGIGVEHPEYDLLPSDYFRRQVYACFWFERSAALGAIERYPDNIL
                     YETDFPHPTCQHPGPRTPAQRPRDYATEALGGLDDDVLAKVLHGNAAALYG"
     gene            1420..2121
                     /locus_tag="VFP02_09740"
     CDS             1420..2121
                     /locus_tag="VFP02_09740"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET9543354.1"
                     /translation="MAALVGITTYPPNAADRYELPREYVAAVRRAGAIPVLIAPGEPR
                     IDDLLERVDGLVLAGGGDIDPTRWGGEAHPTVEFVDAARDDLELDLARRVLDEGIPTL
                     AICRGMQVVNVALGGTLHPHLPETFDGPISHRQEPKWSHTHEIELEPGSLVAEVMGAS
                     VVDPAGWHHQAVDRPGSGLAVTGWSPDGVIEAVELAGHPWLVAVQWHPEMTAADDQTQ
                     QRLFDGLVEVITGQD"
     gene            2126..>2623
                     /locus_tag="VFP02_09745"
     CDS             2126..>2623
                     /locus_tag="VFP02_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9543355.1"
                     /translation="MRLDGKVALITGAASGIGLATARRFAAEGAVVVCADLDGEGAER
                     TAAELTEAGGRATGLTADVSSAADAERMVAVAEETYGALHVAFNNAGIMLSADGDAES
                     TDEGVWDTTMAVNLKGVFLGCKHQIPAMRRAGGGSIINTASFVALMGAATAQLAYTAS
                     KGGVLA"
BASE COUNT          383 a          921 c          952 g          367 t
ORIGIN      
        1 cccgtagagg gcggcggcgt tgccgtggag gaccttggcc aggacgtcgt cgtccagccc
       61 gccgagggcc tcggtcgcgt agtcgcgggg tcgctgggcc ggcgtgcggg gacccgggtg
      121 ctggcaggtg gggtgcggga agtcggtctc gtagaggatg ttgtcgggat agcgctcgat
      181 ggccccgagc gcagccgacc gctcgaacca gaagcaggcg tacacctgcc ggcggaagta
      241 gtccgacggc agcaggtcgt actcggggtg ctcgacgccg atgccgccgt tgcgccactg
      301 ccagtcgaag gcctcgagga cgccgggcag ccagcccacg cccgactcca ccgacacgaa
      361 gcggacctcg gggaagcggt ggcacacgcc cccgaagaac agatcaccca ggcaccgcat
      421 gttgtcgagg atgagcatgg ccgactcctt gccgagattg gcctgcagcc cgatgccccc
      481 gatgtcgtcc atgaggttgc ccaggccccc acccccgatg tggaagctga ccgacaggcc
      541 ggtgtcgcgg gccgcggccc agatggggtc ccagtgcgtg gacccgagcg ggggctcgtc
      601 gtagttctgg ggctggctgc acatgttcac cgcccggtgg cccatggccg ccgcccgctc
      661 gatctcggtc acggcgaact cgaggtccca gaacggcgtg gcgatcacgg gtagaaggcg
      721 gtccggcgcg gccgagcacc actccgtcag gaagtcgttg taggcccgga cgctggcggc
      781 cacgacctcc cggtcgccga ggcggaggaa gaagccgttc ccgaacccgc cgacgttggg
      841 gtagaggacc tgggcctgga tcccctcccg gtccaggaac gccagtcggg cctcggcgtc
      901 gtaggccgcc ggcgggatct cggtgaagtc cttgggcatg tcggggatga cgccgtcgta
      961 cccggccatc gaataggcgc cgggtgcgcc gatgaactcg tcgccggcca cccacatctc
     1021 cagatcgccg cggcgcacga tgcggggcac gtgctcctgc atggccgcgg ggacccgcga
     1081 cgtccacagg tcgggcggct cggtcaggtg ggtgtcgacg tcgatgacgg cgaaacggtc
     1141 gaacagatcg gtcatggcca tccccctcgg aggccgacgc tagtgatccg gtcggcccgg
     1201 cggttccgta cgggactgag ccatgacctt gaccctgctc gccccgaccg ggacccccgc
     1261 ggccgggata cgttctcggc ccgtgcccct ggcacctgtc accgacgact gcctcctgcg
     1321 cgaccggctc gtggctccgt tccgactgcg cagcctcccg acctggctca cttctctcgt
     1381 cgagtgcggc gattcgccac tgcgtggtag tcatcccgga tggctgcgct ggtcgggatc
     1441 acgacgtacc ccccgaacgc ggccgaccgc tacgagttgc cccgagagta cgtggccgcg
     1501 gtgcgacggg cgggggccat cccggtcctg atcgccccgg gtgagcctcg catcgacgac
     1561 ctcctggagc gggtcgacgg cctggtcctg gcggggggcg gcgacatcga ccccacccgc
     1621 tggggcggtg aggcccaccc gacggtcgag ttcgtggacg cggcccgcga cgacctggag
     1681 ctggacctgg cccggcgggt gctggacgag ggcatcccca cgctggccat ctgccggggg
     1741 atgcaggtcg tgaacgtcgc cctcggcggg accctccacc cccacctgcc cgagaccttc
     1801 gacgggccga tctcgcaccg ccaggagccg aagtggtccc acacccacga gatcgagctc
     1861 gaaccgggct cgctggtcgc cgaagtgatg ggcgcctcgg tcgtcgaccc cgcgggctgg
     1921 caccaccagg ccgtcgaccg accggggagc ggcctggcgg tcacgggatg gtcgcctgac
     1981 ggagtcatcg aggccgtgga gctggccggc cacccctggc tggtcgccgt gcagtggcac
     2041 ccggagatga ccgcggccga tgatcagacc cagcaacgac tcttcgacgg cctcgtcgag
     2101 gtcatcacgg gacaggactg agcacatgcg actggacggg aaggtggcgc tcatcacggg
     2161 cgcggcatcc gggatcgggc tcgcgacggc ccgccgcttc gccgccgagg gagccgtggt
     2221 ggtctgtgcc gacctcgacg gtgagggggc ggagcgcacc gcggccgagc tgaccgaggc
     2281 cggcggtcga gccaccgggc tcacggctga cgtttcgtcc gcggccgacg ccgagcggat
     2341 ggtggccgtg gccgaggaga cctacggcgc cctgcacgtg gccttcaaca acgccgggat
     2401 catgctgagc gccgacggcg acgccgagtc gactgacgag ggcgtctggg acacgaccat
     2461 ggccgtcaac ctcaagggtg tcttcctcgg ctgcaagcac cagatccccg ccatgcgccg
     2521 ggcgggcggc ggctcgatca tcaacaccgc ctccttcgtc gccctcatgg gcgcggcgac
     2581 cgcccagctc gcgtacaccg cgtcgaaggg gggcgtgctg gcc
//
LOCUS       DASTFV010000531         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1961909, whole genome shotgun sequence.
ACCESSION   DASTFV010000531 DASTFV010000000
VERSION     DASTFV010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1961909"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VFP02_09750"
     CDS             <1..453
                     /locus_tag="VFP02_09750"
                     /inference="COORDINATES: protein motif:HMM:NF039954.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5990 family protein"
                     /protein_id="HET9543356.1"
                     /translation="RGPGVRVQIRGRDLPGRTWCGDGEDHDNVHVGLQVRSDPVDLVP
                     GDADSASWTLEVRVKVAEDGALDFAGPAVHGRRGERFFYLTWSDVGSDGGFAMFRRAK
                     LILDRVDPALVRSVEDDGQPLIGEISLTDDRGGPRCARVDQPALSWSV"
     gene            complement(503..1237)
                     /locus_tag="VFP02_09755"
     CDS             complement(503..1237)
                     /locus_tag="VFP02_09755"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9543357.1"
                     /translation="MTDGWYRTFFDQAANDHWNAALPESHTEREATFLVDQLDLKAGD
                     TVLDLPAGRGRLTLPLADRGMRMIAADISDDGTRGLRDAVGTRPVVVVRADMRAVPLR
                     TQVDAAFCMGNSFGYFPVDGVERFLGEVTRLLRPGGRFVLESATVAESMTASFAERTE
                     HDFGGVRVVAHHRLDPEHDRVVSELEVTDTAGVRTLAIDQLVLPSARIAGLVEAAGLQ
                     VSALLGSTDGAPFDATAGSLLVVAHH"
     gene            complement(1234..1548)
                     /locus_tag="VFP02_09760"
     CDS             complement(1234..1548)
                     /locus_tag="VFP02_09760"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HET9543358.1"
                     /translation="MTGHRPTPPRPLRGSLEALLDDLGAAPVRETNSLLTRWAEVVGR
                     DMAAHTEPVGIRNGVLVVHTDDPAFGQSLEWREAEFLQRLAVVLGEGVVTGLQVRIRP
                     PS"
     gene            complement(1545..>2426)
                     /gene="recF"
                     /locus_tag="VFP02_09765"
     CDS             complement(1545..>2426)
                     /gene="recF"
                     /locus_tag="VFP02_09765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HET9543359.1"
                     /translation="ASAVVRGEVERDGRELLIECEISGGRTVAQLNRQRGARRRDLLD
                     ALRVSIFTPDDLVLVKGGPGERRRFLDDAMVQLRPRDDALRLELERVLRQRNTLLKQA
                     GGRLTDEVGLTLDVWDQKLTEVGEAIDARRVEIAAALAPLVSEAYGRLAGLPVVVEVS
                     HRSSWADAASLAEALAAARDEDVKRRVSTVGPHRDDVDLILADLPARTQASQGEQRSL
                     ALALRLGVHRLVADRVGVPPVLLLDDVFSELDAGHSRALIELLPEGQALLTTAGELPP
                     GIEPERRLSLAGGKILP"
BASE COUNT          327 a          862 c          911 g          326 t
ORIGIN      
        1 cgaggtcctg gtgtgagggt gcagatccgg ggtcgggacc tgcccgggag gacgtggtgc
       61 ggtgacggcg aggaccacga caacgtgcac gtcggcctcc aggtccgtag cgacccggtc
      121 gacctcgtcc ccggggacgc ggactcggcc tcgtggaccc tggaggtgcg ggtgaaggtg
      181 gcggaggacg gcgcgctcga cttcgccggc cccgccgtgc acggccgccg gggtgagcgc
      241 ttcttctacc tgacctggag cgacgtcggc tccgacggtg ggttcgccat gttccgccgg
      301 gccaagttga tcctcgaccg cgtcgacccc gccctcgtgc ggtccgtcga ggacgacgga
      361 cagccgttga tcggcgagat ctcgctcacc gacgaccggg gcggtccgag gtgcgctcgg
      421 gtcgaccaac cggcgctcag ctggtcggtc tgagccgagc ccggggcgga cctcggcacc
      481 gaccagcccg cgacggcagg gttcagtggt gggcgacgac gagcagggag ccggcggtgg
      541 cgtcgaaggg cgcgccgtcg gtactgccga ggagggcgct cacctgcagt ccggcggcct
      601 cgacgaggcc ggcgatacga gccgagggca ggacgagttg gtcgatggcc agcgtccgca
      661 ccccggcggt gtcggtgacc tcgagctcgc tcacgacccg gtcgtgctcg gggtccaggc
      721 ggtggtgggc cacgacccgc acgccgccga agtcgtgttc ggtcctctcg gcgaacgacg
      781 cggtcatcga ctcggccacg gtcgccgact ccagcacgaa ccggcccccc gggcgcagga
      841 ggcgcgtcac ctcgcccaga aaccgctcga ccccgtcgac ggggaagtac ccgaagctgt
      901 tgcccatgca gaaagcggcg tcgacctggg tccgcagggg cacggcccgc atgtccgccc
      961 ggaccacgac caccggccgg gtgccgaccg cgtcccgaag gccgcgtgtg ccgtcgtcgg
     1021 agatgtcggc cgcgatcatc cgcatgccgc ggtcagccag cggcagggtc aaccggcctc
     1081 ggccggcggg caggtccagc accgtgtcgc cggctttcag gtcgagttga tccaccagga
     1141 aggtcgcctc gcgctccgtg tgggactcgg gcagtgcggc gttccagtgg tcgttggccg
     1201 cctggtcgaa gaaggtgcgg taccagccgt cggtcacgag gggggtcgga tccggacctg
     1261 gagcccggtc accacgccct cgcccagtac gacggccagg cgctggagga actcggcctc
     1321 ccgccattcc agcgactggc cgaacgcggg gtcgtcggtg tgcacgacca agaccccgtt
     1381 cctgatcccg acgggctcgg tgtgggcggc catgtcccgc cccacgacct cggcccagcg
     1441 ggtcagcagg gagttggtct cgcggaccgg cgccgctccg aggtcgtcga gcaatgcctc
     1501 cagcgaaccc cggagggggc gcggcggggt gggtcggtgg ccggtcacgg gaggatcttc
     1561 ccacccgcca gggacagccg ccgctccggc tcgatccccg ggggcagctc ccctgccgtg
     1621 gtcaggaggg cctggccctc gggcaggagc tcgatcagcg cccggctgtg gcccgcgtcg
     1681 agctcggaga acacgtcgtc gaggagcagg accggcggca cgccgacccg gtcagcgacc
     1741 aggcggtgca cgccgagacg cagcgccagg gccagggacc gctgttcgcc ctgggaggcc
     1801 tgcgtgcgcg ccggcaggtc ggcgaggatc aggtcgacgt cgtcacggtg ggggccgacg
     1861 gtcgacaccc gccgcttcac gtcctcgtcg cgggccgcag ccagggcctc ggcgaggctc
     1921 gcggcgtcgg cccatgacga tcggtggctg acctcgacga cgaccggcaa gccggcgagg
     1981 cgcccgtagg cctcgctgac cagtggcgcg agggcggcgg cgatctcgac gcggcgggcg
     2041 tcgatggcct cgcccacctc ggtcagcttc tggtcccaca cgtcgagggt cagcccgacc
     2101 tcgtccgtga gtcggccgcc cgcctgcttg agcagggtgt tgcgctgtcg caggacccgc
     2161 tccagttcga ggcggagggc atcgtctcga ggtcggagtt ggaccatggc gtcgtccagg
     2221 aaccgccgac gctcccccgg cccgcccttc accaggacca ggtcgtccgg ggtgaagatg
     2281 ctgacgcgga gcgcgtccag caggtcccgg cgacgggctc ctcgctggcg gttgagctgg
     2341 gcgacggtgc ggccaccgct gatctcgcac tcgatgagca gctcgcgccc gtcccgctcg
     2401 acctcgcccc gcaccaccgc ggacgc
//
LOCUS       DASTFV010000532         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1967885, whole genome shotgun sequence.
ACCESSION   DASTFV010000532 DASTFV010000000
VERSION     DASTFV010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967885"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VFP02_09770"
     CDS             <1..423
                     /locus_tag="VFP02_09770"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9543360.1"
                     /translation="TASRSSIYEWYGAGADQVHVEEFDFVADHPTLGHGHGPTPQEYV
                     LHALAACITAGVATGAAARNIELQKVSSKVSADIDVRGVLGIDPDIRKGFSDVRVEFD
                     VEADAEPAVIDALVASATKYSAVYDMLTSPTSVTVTRA"
     gene            complement(451..1839)
                     /locus_tag="VFP02_09775"
     CDS             complement(451..1839)
                     /locus_tag="VFP02_09775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9543361.1"
                     /translation="MTATDERSVSWWMETCGDDLTPRPPLDGSTTADVAVLGAGYTGL
                     WTALYLLERDPSLNVVLVEREIAGWGASGRNGGWLSPEISVSHTEMIARHGKDATRAT
                     HDALIDTIGHVESAVERHGIDAHLDRHGMLMLARGGHQVKTVRHIYDEMTELGYPDFY
                     RWLEPGEAAELVDVDNTWAALFAEPFAAVHPGRLVRGLARAVEGLGATIYEGTAVTGY
                     DLTAKPVLHTTGGDITADVIVLAGEAYLSELPKHGREILPVYSQIMLTAPLTEAQRAT
                     IGWRRRFVMQSKRLTIDYLQITRDNRIAFGGRGSLYHWGSAIKREWDCVEATAQSLRS
                     QFDEFFPSLADVPFERCWGGVVGIHRDWMPSFSFDQATGLAKAGGYAGEGVAATNLAG
                     RTLADLITGGGTPLCELPMVQHEPKAWEPEPLRYLGVRYIQKAAARMEEKAVRTGKAP
                     TGRSIAERLMSH"
     gene            1873..>2407
                     /locus_tag="VFP02_09780"
     CDS             1873..>2407
                     /locus_tag="VFP02_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543362.1"
                     /translation="MAPMRSDEPVLVGVGQHVHRPDDGPVPSLVEMMTTAARRAGGDV
                     GPGVLDRLGWIGVPKGAWTHPDPGREVAAAVGAPDAHTVLVEVGITQQEVIDVALAAV
                     QDGAGPALVVGAEVNHGSTAGAATAVGTARRAAVEPDEHLTAHDLGVSAVEMNQLFID
                     PPTVYAVLENAFAAAQGW"
BASE COUNT          327 a          852 c          877 g          351 t
ORIGIN      
        1 accgccagtc ggtcctcgat ctacgagtgg tacggcgccg gtgccgacca ggtccacgtg
       61 gaggagttcg acttcgtcgc cgaccatccg acgctcggcc atgggcacgg ccccacgccc
      121 caggagtacg tgctccacgc cctcgccgcc tgcatcaccg ccggcgtggc caccggcgcg
      181 gccgcccgga acatcgagct ccagaaggtc tcgtccaagg tcagcgccga catcgacgtc
      241 cgcggtgtgc tcgggatcga ccccgacatc cgcaaggggt tcagcgacgt gcgggtcgag
      301 ttcgacgtcg aggcggacgc ggagccggcc gtcatcgacg cgctcgtggc gtcggccacc
      361 aagtactccg cggtctacga catgctgacg agccccacgt cggtcaccgt cacgcgagcc
      421 tgaccccggg cccgcgggtt cgacccgcgg tcagtgcgac atcaggcgtt cggcgatgct
      481 ccggccggtg ggagccttgc cggtacggac ggccttctcc tccatgcggg cggccgcctt
      541 ctggatgtag cggaccccga ggtagcgcag aggctcgggc tcccaggcct tgggctcgtg
      601 ctggaccatc ggcagctcgc agaggggtgt gcccccgccg gtgatcagat cggccagggt
      661 ccggccagcc aggttggtgg cggccacccc ctcgccggcg tagcccccgg ccttggccag
      721 gcccgtcgcc tggtcgaagc tgaacgacgg catccagtcc cggtggatgc cgaccacacc
      781 gccccagcag cgttcgaagg gaacgtcggc cagggacggg aagaactcgt cgaactggct
      841 ccgcaggctc tgggccgtgg cctccacgca gtcccactcc cgcttgatgg ccgagcccca
      901 gtggtagagc gagccccggc cgccgaaggc gatgcggttg tcccgggtga tctgcaggta
      961 gtcgatggtg aggcgcttgg actgcatgac gaagcggcgg cgccagccga tcgtggcccg
     1021 ctgggcctcg gtcagcgggg cggtgagcat gatctgggag tagacgggga ggatctcccg
     1081 gccgtgcttc gggagctcgg agaggtaggc ctcgccggcc aggacgatga cgtcggccgt
     1141 gatgtcgccg cctgtcgtgt gcagcaccgg cttcgcggtc aggtcgtagc cggtgacggc
     1201 ggtgccctcg tagatggtgg cgccgaggcc ttcgaccgcc cgggccaggc cccgcaccag
     1261 gcgaccgggg tgcacggctg cgaacggctc ggcgaacagc gcggcccagg tgttgtcgac
     1321 atcgaccagc tcggcggcct cgcccggctc cagccagcgg tagaagtccg ggtacccgag
     1381 ctcggtcatc tcgtcgtaga tgtggcggac ggtcttcacc tggtggccgc cccgggccag
     1441 catcagcatc ccgtggcggt cgaggtgggc gtcgatgccg tggcgctcga cggccgactc
     1501 gacgtggccg atggtgtcga tcagggcgtc gtgggtggcc cgggtcgcgt ccttgccgtg
     1561 gcgggcgatc atctcggtgt ggctgaccga gatctccggt gacagccagc cgccgttgcg
     1621 acccgaggcc ccccagcctg cgatctcccg ctcgacgagc acgacgttca ggctcggatc
     1681 ccgttccagc aggtagagcg cggtccacag tcccgtgtag cccgcgccca ggacggccac
     1741 gtcggcggtg gtcgagccgt ccagcggcgg gcgcggggtc aggtcgtcac cacaggtctc
     1801 catccaccag ctgaccgagc gctcgtccgt ggccgtcatg ggggcgatca tggcaccact
     1861 accgtccggg ccatggcccc gatgcgctct gatgagcccg ttctggtcgg ggtgggccag
     1921 cacgtccacc gccccgacga cggcccggtg ccgtccctcg tcgagatgat gaccacagcc
     1981 gcccgtcggg cggggggtga tgtcgggccc ggggtcctcg accgcctcgg ctggatcggc
     2041 gtgcccaaag gggcgtggac ccaccccgat ccgggccggg aggtggcggc cgcggtcggc
     2101 gcgcccgacg cccacaccgt cctcgtcgag gtcggcatca cccaacagga ggtgatcgac
     2161 gtcgccctcg ccgccgtgca ggacggggcc ggcccggccc tggtcgtggg ggccgaggtc
     2221 aatcacgggt cgacggccgg cgcggcgacc gcggtcggta cggcgcggcg ggcggcggtc
     2281 gagcccgacg agcacctgac cgcccatgac ctgggcgtca gcgcggtgga gatgaatcag
     2341 ctcttcatcg acccgcccac cgtctacgcc gtcctggaga acgccttcgc cgcggcccag
     2401 ggctggg
//
LOCUS       DASTFV010000533         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1968740, whole genome shotgun sequence.
ACCESSION   DASTFV010000533 DASTFV010000000
VERSION     DASTFV010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968740"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1288)
                     /locus_tag="VFP02_09785"
     CDS             complement(68..1288)
                     /locus_tag="VFP02_09785"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET9543363.1"
                     /translation="MTAVMQGVRVLEVAEHTFVPAASALLADWGAEVIKVEHVERGDA
                     MRGLASSGTVSLGGGVHVLLEHSNRGKQSIGLDLTSEDGLEILGKLAATCDVFLTNKM
                     PSVRTKLNVDVDDIRAHNPSIIYVRGTGQGERGPDADKGSYDSLAYWNRAGIAMGQKQ
                     ADEDYVPGPPAPAFGDSIGAMTIAGGIMGALFHRERTGEATTVDVSLLATGLWSMGAA
                     LALSLQLDVPWAPPPKGGSPSGNPLVANYRTKDGRYVALCCLQPGKYWPEAVALIGRP
                     ELADDERFADAPTILANGDEGYELLAAAFAERTLDEWREQLADFSGQWTIVQNTLEAA
                     ADPQSVANGYVSDCETADGVPFRLATAPVQYDGQPAQALRAPEFNEHGDAILESLGYD
                     WDTIVDLKVRGVVA"
     gene            1415..>2587
                     /locus_tag="VFP02_09790"
     CDS             1415..>2587
                     /locus_tag="VFP02_09790"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9543364.1"
                     /translation="MARPLNFPVFDADNHLYETQDSFTRHLPDRYRGAIDYVDVRGRT
                     KIVVRGQISEYIPNPTFEVVARPGAQEEYFRVGNPEGKSYRELVGEPMRSIPAFREPA
                     PRIELMDELGIDRALMFPTLASLLEERMRDDADLIHAVVHSLNEWLYEQWSFNYEDRI
                     FTTPVITLPIVEKAIEELEWAVERGAKTILVRPAPVPGYNGSRSFGFEEFDPFWQAVV
                     DADILVSMHASDSGYSRYQADWTGPQEMLPFRPNAFRMMTMGKRPIEDAMSALVCHGV
                     LTRFPDLRVAAIENGGDWVVPFLHHLEDVHRKMPHAFDEDPIEAFRRNIYVSPFHEDD
                     LGALFDAIGTDHVLFGSDYPHPEGLAEPCSYLDHLPSGLSDEVVQGIMGGNLAKIMK"
BASE COUNT          390 a          903 c          880 g          414 t
ORIGIN      
        1 cgccgtcggg accacggatc cgtggtgtgc cgggcgcatg ttcgtggtgg ccgagcgctc
       61 ctcggcgtca ggccacgacc ccgcggacct tgaggtccac gatcgtgtcc cagtcgtagc
      121 cgaggctctc gaggatggca tcgccgtgct cgttgaactc gggggctcgc agggcctgcg
      181 cgggctggcc gtcgtactgc acgggggccg tggccagccg gaacgggacg ccgtcggccg
      241 tctcgcagtc gctgacgtag ccattggcga ccgattgcgg gtcggccgcg gcctcgagcg
      301 tgttctgcac gatcgtccac tgcccgctga agtcggccag ctgctcccgc cactcgtcga
      361 gcgtgcgctc ggcgaaggca gcggccagga gctcgtagcc ctcgtcgccg ttggcgagga
      421 tcgtgggagc gtcggcgaag cgctcgtcgt cagccagctc cggccgcccg atgagggcga
      481 cggcctcggg ccagtacttc cctggctgca ggcagcagag ggccacgtag cggccgtcct
      541 ttgtgcggta gttggcgacg agggggttgc cgctcgggct cccgcccttc ggcggtgggg
      601 cccacggcac gtcgagctgc agggagagag ccagggcggc gcccatcgac cacaggccgg
      661 tggccaggag cgagacgtcg acggttgtgg cctcgccggt gcgctcccgg tggaacagcg
      721 cgcccatgat cccgccggcg atggtcatgg ccccgatgga gtcaccgaat gccggggccg
      781 gggggccggg cacgtagtcc tcgtcggcct gcttctgacc catggcgatg cccgcccggt
      841 tccagtaggc gagcgagtcg taggagccct tgtcggcgtc ggggccccgc tcaccctgac
      901 ccgtgccccg cacgtagatg atgctcgggt tgtgggcgcg gatgtcgtcg acatcgacgt
      961 tcagcttggt gcggaccgac ggcatcttgt tggtcaggaa gacgtcacag gtcgccgcca
     1021 gcttgccgag gatctcgagc ccgtcctcgg acgtgaggtc gaggccgatg ctctgcttgc
     1081 cccggttgga gtgctccagc agcacgtgga ccccaccgcc gaggctgacg gtgccgctcg
     1141 acgcgaggcc gcgcatggcg tcgccccgct cgacgtgctc gaccttgatc acctcggcgc
     1201 cccagtcggc gaggagggcc gatgcggcgg ggacgaaggt gtgctcggcg acctccagga
     1261 cccggacacc ctgcatgact gcggtcatcg ctcctcctgg acagctcagc ggcagatgac
     1321 gccgatgtta gacagagatg gcgaccgatg cccaacggag cggagcacga cgccaaagtt
     1381 gacgtgagtg ttagagaaac cgtatcctgg tccgatggct cgcccgttga acttccccgt
     1441 cttcgacgct gacaaccacc tgtacgagac ccaggactcg ttcacccggc acctgccgga
     1501 ccggtaccgg ggcgccatcg actacgtcga tgtccgcggc cggaccaaga tcgtggtccg
     1561 cggtcagatc agcgagtaca tcccgaaccc gaccttcgag gtcgtggccc ggcccggcgc
     1621 ccaggaggag tacttccgcg tgggcaaccc ggagggcaag tcctatcggg agctggtcgg
     1681 cgagccgatg cgctccatcc cggccttccg tgagccggcc ccccgcatcg agctgatgga
     1741 cgagctcggc atcgaccggg ccctcatgtt cccgaccctc gccagcctgc tcgaggagcg
     1801 gatgcgagac gatgccgacc tcatccacgc cgtcgtccac tccctcaacg agtggctcta
     1861 cgagcagtgg tcgttcaact acgaggaccg gatcttcacc acaccggtca tcaccctccc
     1921 gatcgtcgag aaggccatcg aggagctcga gtgggcggtc gagcgcgggg ccaagaccat
     1981 cctcgtccgc ccggcgccgg tgccgggcta caacggctcc cggtcgttcg gcttcgagga
     2041 gttcgacccg ttctggcagg cggtcgtcga cgccgacatc ctcgtgtcca tgcacgcttc
     2101 cgacagcggc tactcccgct accaggccga ctggaccggc ccccaggaga tgctgccgtt
     2161 ccggcccaac gccttccgca tgatgacgat gggcaagcgg ccgatcgagg acgccatgtc
     2221 ggcgctggtc tgccacggcg tgctcacccg cttccccgac ctgcgggtgg cggccatcga
     2281 gaacgggggc gactgggtcg tccccttcct ccaccacctg gaggacgtcc accggaagat
     2341 gccgcacgcg ttcgacgagg acccgatcga ggccttccgg cgcaacatct acgtcagccc
     2401 gttccacgag gacgacctcg gggccctgtt cgacgccatc gggaccgacc acgtcctgtt
     2461 cggctccgac tacccccacc ccgagggcct ggccgagccg tgcagctacc tcgatcacct
     2521 gccttcgggc ctgtccgacg aggtcgtgca ggggatcatg ggtggcaacc tggccaagat
     2581 catgaag
//
LOCUS       DASTFV010000534         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1975400, whole genome shotgun sequence.
ACCESSION   DASTFV010000534 DASTFV010000000
VERSION     DASTFV010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1975400"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFP02_09795"
     CDS             complement(<1..332)
                     /locus_tag="VFP02_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HET9543365.1"
                     /translation="MTLQALVFDFDGLILDTEWSAFSTAAEVWTEHGVELDLAVWQEI
                     VGTADHVHWSEMLENDLGRPIDRDTVVPARQAQHHVEIEAMDLLPGVVDLIEAAVTAG
                     LALAVASSS"
     gene            complement(329..1789)
                     /gene="cobA"
                     /locus_tag="VFP02_09800"
     CDS             complement(329..1789)
                     /gene="cobA"
                     /locus_tag="VFP02_09800"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR01469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HET9543366.1"
                     /translation="MTVYLVGAGPGDPGLLTVRGAEVLARADVVVYDRLSVAALLDQA
                     PAHAERISVAKQPGGTSTPQHEINRLLVEHGRAGRTVVRLKGGDPFVFARGGEEAQAL
                     LDAGVPFEVVPGITSAIAAPAYAGIPVTLRHSSTSFTVVTGHEDPTSGAEGTVNWEAV
                     AQVGGTIVILMGVARMGLIAERLIAGGRSPDTPAAAVQWGTRPEQRTIRATLATIADQ
                     DLAAPSTIVVGEVAAEDLAWFAARPLFGQTVVVTRPEGQASVLAERLRAAGAEAVVLP
                     VIEIEEPADGGTALTAALDDLGAYDWLVVTSANGARRVLAGLPDARALGGVSVAAIGP
                     GTAAALAEGNIRADLVPDRYVAEGLLEAFPAPPEGGGLVLLARAAVARDTLPEGLRAA
                     GWSVDVVEAYRTVPASPDPAALDAAAAAGVVTFTSSSTVTRFLEVAGRDRVPPLVACI
                     GPVTAATARDAGLTVDVEATDHTVPGLVAALVEHLR"
     gene            complement(1786..>2144)
                     /locus_tag="VFP02_09805"
     CDS             complement(1786..>2144)
                     /locus_tag="VFP02_09805"
                     /inference="COORDINATES: protein motif:HMM:NF015835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543367.1"
                     /translation="LDPAIVLPQAGQGTLAVECRADDEATRALLAALDHEPSHRTLRA
                     ERAFLAELGGDCDIPAGAYAVVDGDGLKVEGLIASLDGHVVLRHTVTGADPEATGRTV
                     ARFLLDEAGGSAILQP"
BASE COUNT          276 a          772 c          837 g          259 t
ORIGIN      
        1 gagctggagg ccacggccag ggccaggccg gcggtcaccg cggcctcgat caggtcgacg
       61 acgccgggca ggaggtccat ggcctcgatc tcgacgtggt gctgggcctg ccgggcgggc
      121 acgacggtgt cgcggtcgat gggccggccg aggtcgttct ccagcatctc gctccagtgg
      181 acgtggtcgg cggtgccgac gatctcctgc cacacggcga ggtccagctc gacgccgtgc
      241 tcggtccaga cctcggccgc ggtcgagaag gccgaccact cggtgtcgag gatgaggccg
      301 tcgaaatcga agaccagggc ctgcagcgtc accggaggtg ctcgacgagg gcggcgacca
      361 gccctgggac cgtgtggtcg gtggcctcga cgtccaccgt caggccggcg tcccgggccg
      421 tggccgcggt cacggggccg atgcaggcca cgagtggcgg gacccggtcc cggccggcga
      481 cctcgaggaa gcgggtcacg gtcgaggacg aggtgaacgt cacgacgccg gcggccgccg
      541 ccgcgtccag cgccgcggga tctgggctcg cgggaacggt gcggtaggcc tcgaccacgt
      601 ccacgctcca gcccgcggcc cgcagcccct cggggagggt gtcgcgggcc accgcggcgc
      661 gggccagcag gaccaggcct cctccttctg gaggagccgg gaatgcctcg agcaggccct
      721 cggccacata gcggtcgggg acgaggtcgg cccggatgtt gccttcggcc agtgccgcgg
      781 cggtacccgg cccgatggcg gcgaccgaca ccccgcccag cgcccgggcg tcggggaggc
      841 cggccagcac ccgccgagcc ccgttcgccg aggtcaccac cagccagtcg taggcgccga
      901 ggtcgtccag ggccgccgtc agcgccgttc cgccgtcggc gggttcctcg atctcgatca
      961 ccgggaggac gacggcttcg gccccggccg cccgcaggcg ctcggccagg acgctggcct
     1021 ggccctcggg ccgggtgacc acgacggtct gcccgaagag cgggcgggcc gcgaaccagg
     1081 ccaggtcctc ggccgccacc tcgcccacga cgatggtgga cggggcggcc aggtcctggt
     1141 cggcgatcgt cgccagcgtg gcccggatcg tgcgctgctc ggggcgggtc ccccactgga
     1201 ccgcggcggc cggcgtgtcc ggcgagcgtc caccggcgat gaggcgctcg gcgatgaggc
     1261 ccatgcgggc cacgcccatg aggatgacga tggtgccgcc gacctgggcc accgcctccc
     1321 agttgaccgt gccctcggcg cccgacgtgg ggtcctcgtg gccggtgacc acggtgaagg
     1381 acgtcgacga gtggcgcaag gtcaccggga tcccggcgta ggccggggcg gcgatggccg
     1441 acgtgatgcc ggggacgacc tcgaagggga cgccggcgtc gagcagggcc tgggcctcct
     1501 cgccgccacg ggcgaagacg aagggatcgc cgcccttgag gcgcacgacg gtgcggccgg
     1561 cccggccgtg ctcgaccagc aggcggttga tctcgtgttg cggggtcgac gtgccgccgg
     1621 gttgcttggc cacgctgatg cgttcggcgt gggccggggc ctggtcgagc agtgccgcga
     1681 ccgacagccg gtcgtagacg accacgtcgg cccgggccag cacctcggcc ccccgcacgg
     1741 tgagcagtcc cgggtccccc ggccccgccc ccaccaggta gacggtcatg gctgaaggat
     1801 ggccgatcca ccggcctcgt cgagcaggaa tcgggccacg gtccggcccg tggcctcggg
     1861 gtcggcaccg gtgacggtgt ggcggaggac gacgtggcca tccagcgacg cgatcaggcc
     1921 ctcgaccttc aggccgtcac cgtcgacgac ggcgtaggcc ccggccggga tgtcgcagtc
     1981 gccgccgagc tcggccagga aggcccgctc ggcccgcagg gtccggtggc tgggctcgtg
     2041 gtcgagagcg gccagcaggg cccgggtcgc ctcgtcgtcg gcccggcact cgacggccaa
     2101 ggtcccctgg cccgcctggg gcaggacgat cgcggggtcc agga
//
LOCUS       DASTFV010000535         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1975861, whole genome shotgun sequence.
ACCESSION   DASTFV010000535 DASTFV010000000
VERSION     DASTFV010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1975861"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFP02_09810"
     CDS             complement(<1..518)
                     /locus_tag="VFP02_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543368.1"
                     /translation="MRSRLTTLRARIVAAPLESWITAAVVVGAVAFTFAQLHPNLIFR
                     NTTPTGGDMGAHVWSGTYLRDHILTHGRLSGWTPDWYAGAPAFHFYMVVPFLLILLLD
                     VLLPYAVAFKLVAVSGVVLLPAAAWAFGKLSGLRFPGPPMLAAATVFFLFDTRFTIYG
                     GNIASTMAGEFA"
     gene            complement(589..780)
                     /locus_tag="VFP02_09815"
     CDS             complement(589..780)
                     /locus_tag="VFP02_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543369.1"
                     /translation="MSMVEERCPGCGRAYVEISLEVGGRMMTMRSCSNCDVRQWHTPD
                     GEKALDGVLADISASTGRR"
     gene            complement(877..1455)
                     /locus_tag="VFP02_09820"
     CDS             complement(877..1455)
                     /locus_tag="VFP02_09820"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9543370.1"
                     /translation="MIAVLQIPLWAGYLGMPLYAARRKGNGVVADFGFRMEWLDIPKG
                     IGAGLGTQIVLIPLLYAVLFRITDAVGWEFDHDLSAEARHLSDKATDWAGVVLLIVIV
                     AVLAPIIEELFFRGLFLRAIEKRWGPRWALWVSSLVFAAVHLQLLQFPALALIGLVLG
                     WLTLRTGRLGPAVWAHIAFNAVATGILLAEAS"
     gene            complement(1644..1868)
                     /locus_tag="VFP02_09825"
     CDS             complement(1644..1868)
                     /locus_tag="VFP02_09825"
                     /inference="COORDINATES: protein motif:HMM:NF023430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HET9543371.1"
                     /translation="MARRCARPTCSERSVVTLSYDYAGRTVWLNDLVIEDDPANHDLC
                     GRHADRVTAPQGWVLHDRRNVAPIWQAQAS"
     gene            1930..>2462
                     /locus_tag="VFP02_09830"
     CDS             1930..>2462
                     /locus_tag="VFP02_09830"
                     /inference="COORDINATES: protein motif:HMM:NF018218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HET9543372.1"
                     /translation="MAQPPPPPPPPPPSGNKSDGKSGSSTPWPRWSLWVLVGVILASF
                     LLTTLVGGDDGESISYEAFIQKAGEGEVASMTFDNTNAKITGKLEDGTEFSTTGPLES
                     GIPPEDLATLRANDVEIEYDTPSPNPLWSLLSFALPIILIIGFFWWMSRRAQGQMGGI
                     MSIGRSRAKTYSTERPGT"
BASE COUNT          426 a          825 c          892 g          319 t
ORIGIN      
        1 aaggcgaact cgcccgccat ggtcgaggcg atgttgccgc cgtagatggt gaaccgggtg
       61 tcgaagagga agaacaccgt ggccgcggcg agcatcggcg gcccggggaa gcgcagccca
      121 ctcagcttgc cgaaggccca ggcggcggcc gggaggagga ccacgccgct gaccgcgacc
      181 agcttgaagg cgaccgcata gggcaggagg acgtcgagca gcaggatcaa caggaacggg
      241 acgaccatgt agaagtggaa cgccggggcg ccggcgtacc agtcgggggt ccagccgctc
      301 agccggccgt gggtgaggat gtggtcccgc aggtaggtgc ccgaccacac gtgggcgccc
      361 atgtcgccgc cggtcggcgt ggtgttgcgg aagatcaggt tcgggtggag ctgggcgaag
      421 gtgaaggcca ccgcgccgac gacgaccgcg gcggtgatcc aactctccag gggagcggcc
      481 acgatgcggg cgcgcagcgt ggtcaggcgg gaacgcatgg cctcaccatc ctcgcagggg
      541 cgggcccgat tcggacggca ggcggtgaag ccgccgccgt cccgggcttc agcggcgacc
      601 cgtgctcgcg ctgatgtcgg ccaggacacc gtccagggcc ttctcgccgt cgggcgtgtg
      661 ccactggcgg acgtcacagt tcgagcagga ccgcatcgtc atcatccgcc ccccgacctc
      721 gaggctgatc tcgacatagg cgcgcccgca acccgggcac cgctcctcca ccatgctcac
      781 actccgtgat cgcttgcccc acccatcgga gccaaacggg ccggattgag gccattcgcc
      841 acgtgcagtg gcgaatctga tcaccgaacg ccccgttcag ctggcctcgg ccaggaggat
      901 gccggtggcc acggcgttga aggcgatgtg ggcccagacg gcggggccga ggcggccggt
      961 gcgcagggtg agccacccga gcacgaggcc gatgagggcc aacgccggga actgcaggag
     1021 ctgcaggtgg acggcggcga agaccaacga cgagacccac agggcccaac gcggccccca
     1081 ccgcttctcg atggcccgca ggaacagtcc ccggaagaag agctcctcga tgatcggagc
     1141 caggacggcc acgatcacga tgagcaggac cacgccggcc caatcggtgg ccttgtcgct
     1201 caggtggcgg gcctcggcgg acaggtcgtg gtcgaactcc cagcccacgg cgtcggtgat
     1261 gcggaacagg acggcgtaga ggagggggat gaggacgatc tgggtgccca gtccggcacc
     1321 gatgcccttg gggatgtcca gccactccat ccggaagccg aagtcggcga ccacgccgtt
     1381 gcccttgcgc cgggccgcgt agaggggcat ccccagatag cccgcccaga gcgggatctg
     1441 gaggacggcg atcatcgtca ggggcagctc gccgtcggcc ccctcctgga cgtacccggt
     1501 ggcggcgagg atggcggcgc cgatgaagag gccggcggcg ttggcgatga gcagcccgag
     1561 gagcgcgtca ccgacgcccc accggggggt gggaagcgcc gcgggctcgg cgggcagggg
     1621 ttgcgggggc ggcgggaggg agctcagctg gcctgagcct gccagatcgg cgccacgttg
     1681 cgccggtcgt gcaggaccca gccctggggc gcggtcaccc ggtcggcgtg ccgaccgcag
     1741 aggtcgtgat tggccgggtc gtcctcgatg accaggtcgt tcaaccagac ggtgcgaccg
     1801 gcgtagtcgt aggagagcgt gaccaccgac cgctccgagc acgtggggcg cgcgcaacgt
     1861 cgagccatgg agaaaacgct agttcgacgt gacccgggaa tcgtggatac ggacctctac
     1921 gctgggcgga tggcgcagcc tcctccgccc ccgccgcctc ctcccccgag cggcaacaaa
     1981 tccgacggca agtccggcag ctcgacgccg tggccgcgct ggtcgctctg ggtcctcgtc
     2041 ggcgtgatcc tcgcctcgtt cctgctcacc acgctggtcg gcggcgacga cggcgagagc
     2101 atctcctacg aggcgttcat ccagaaggcg ggtgagggcg aggtcgcgtc gatgaccttc
     2161 gacaacacca acgccaagat caccggcaag ctcgaagacg gcaccgagtt cagcaccacc
     2221 ggacccctgg agagcggcat cccgcccgag gacttggcca ccctgcgggc caacgacgtc
     2281 gagatcgaat acgacacccc gtcgccgaac cccctgtgga gccttctctc cttcgccctg
     2341 ccgatcatcc tgatcatcgg gttcttctgg tggatgagtc gccgggcaca ggggcagatg
     2401 ggcgggatca tgtccatcgg ccggtcccgg gccaagacct actccacgga gcggccgggc
     2461 ac
//
LOCUS       DASTFV010000536         2647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1981887, whole genome shotgun sequence.
ACCESSION   DASTFV010000536 DASTFV010000000
VERSION     DASTFV010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1981887"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..807)
                     /locus_tag="VFP02_09835"
     CDS             complement(22..807)
                     /locus_tag="VFP02_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9543373.1"
                     /translation="MTTPKRIAVAGATGRLGSQLVDVLEERGHTVVKASRTYGVDVIT
                     KEGLAEALDDVDAIIDAATQATPDEDAATEFFKTSTANLHEVGHAAGVPRMLTLSIIG
                     TDQFDSGFIGGVNAHEAAAKAGPIPTDIVRASQFHEFVGQLLDWSTQGDVAYLPKMRT
                     QPVSARALAEAIADLVDEPSDGGRTVEIAGPQEELLPDLAGLLIEAEGRPVEVEAVVD
                     PVDGQRHEQGALLPGPDARLVGPTYADWLAARRRFGWAGRMDG"
     gene            890..1747
                     /locus_tag="VFP02_09840"
     CDS             890..1747
                     /locus_tag="VFP02_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9543374.1"
                     /translation="MPEDFLADQFEENRAHLTRVAYRMLGSTAEADDAVQEAWLRLSR
                     SESSEIDNLPGWLTTVTARICLNMLRSRATRREDEWDGHLPDPIITREGDPSDEAELA
                     DSVGLALLVVLEVLPPAERLAFVLHDLFAVPFDEIAEIVDRSPEAARQLASRARRRVR
                     SAQVPDPDVATQRRVVDAFFRAARGGDMSTLVSVLDPSVILRADHGAEGSVEIRGAEK
                     VAANAAMFATPDRIEHPVLVNGQAGVIIEMNGEPFSVMAFTVTDGIIVAIDILADPAR
                     LGGAGIDPR"
     gene            complement(1749..2123)
                     /locus_tag="VFP02_09845"
     CDS             complement(1749..2123)
                     /locus_tag="VFP02_09845"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9543375.1"
                     /translation="MSKIRLGAVSLDCADPGPLSAFWADLLGGEVIVTRDDIGVVKLD
                     HVLLTAMRVDDYVAPTWPTDAVPKQGHFDLEVDDLADAERRAVSLGAVRAPSQPEPES
                     HLVLLDPAGHPFCLSLSANFPR"
     gene            complement(2138..>2647)
                     /locus_tag="VFP02_09850"
     CDS             complement(2138..>2647)
                     /locus_tag="VFP02_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET9543376.1"
                     /translation="SDAKLQTLDPDQKRRAVKVSASVLTSDPWEEACQILPDVNTLTR
                     RAATQAYEQAGVAPEDLDLVELHDCFATAELVHYDNLMLCKEGEAVPFFESGATWRDG
                     STPVNVSGGLESKGHPIAATGIANIWEVCHHLRGEAGDRQIEGAKVGLAHVIGLGSAC
                     GVHILEKAA"
BASE COUNT          400 a          908 c          929 g          410 t
ORIGIN      
        1 aggggaggat cgggatgtcc atcaaccgtc catcctgccc gcccacccga accggcggcg
       61 ggcggcgagc cagtcggcgt aggtcggccc gaccagcctg gcgtcggggc cgggcaggag
      121 ggcaccctgc tcgtggcgct gcccgtcgac ggggtcgacc acggcctcga cctcgaccgg
      181 ccgaccctcg gcctcgatca gcagcccggc gaggtcgggc agcagctcct cctgggggcc
      241 ggcgatctca actgtccgtc cgccgtccga cggctcgtcg accagatcgg cgatggcctc
      301 ggcgagcgcc ctggcgctga ccggttgcgt gcgcatcttg ggcaggtagg ccacatcgcc
      361 ttgcgtgctc cagtccagga gctggccgac gaactcgtgg aactgcgatg cccgaacgat
      421 gtcggtcggg atcggccccg ccttcgccgc cgcctcgtgg gcgtttaccc cgccgatgaa
      481 gcccgagtcg aactggtcgg tgccgatgat ggacagggtg agcatccgcg ggacgccggc
      541 ggcgtggccg acctcgtgca ggttggccgt cgaggtcttg aagaactcgg tggccgcgtc
      601 ctcgtccggc gtcgcctggg tggcggcgtc gatgatcgcg tcgacgtcgt ccagggcctc
      661 cgccagtcct tccttcgtga tgacgtcgac gccgtaggtg cgggacgcct tcacgaccgt
      721 gtggccgcgt tcctcgagca catcgacgag ctgcgagccg agtcgaccgg tggccccggc
      781 aacggcgatc ctcttggggg tggtcatggt cctcttctcc tctgccggtc acaccggcgc
      841 tctctggtgg gtcaaacaca tgacccgcca gcgcgaggag atgtgaccga tgcctgagga
      901 tttcttggcc gaccagttcg aggagaaccg agcccacctg acccgagtcg cctaccgcat
      961 gctcggctcc accgcagagg ccgatgacgc cgtgcaggag gcgtggttgc ggctgagccg
     1021 gtcggaatcg tcggagatcg acaacctgcc gggctggctg accacggtga ccgcgaggat
     1081 ctgcctgaac atgcttcggt cgcgcgccac gcgccgagag gacgagtggg acggccacct
     1141 ccccgacccg atcatcacga gggaaggtga cccgtccgat gaagccgagc tcgccgactc
     1201 ggtcggactg gcgctcctgg tcgtcctgga ggtcctgcca ccggccgagc gcctggcctt
     1261 cgtcctccac gacctcttcg ccgtcccctt cgacgagatc gccgagatcg tcgaccgcag
     1321 cccagaagcc gctcgccaac tggccagccg agcgcgacga cgggtccgaa gcgcgcaggt
     1381 ccccgatccc gacgtcgcca ctcagcggcg ggtcgtcgac gccttcttcc gagcggcgag
     1441 aggcggcgac atgtcgacct tggtgtcggt cctcgacccg agcgtcatct tgcgggccga
     1501 ccatggcgcg gagggcagcg tggagatccg aggcgccgaa aaggtcgccg ccaacgcagc
     1561 catgttcgcc actcccgacc gcatcgagca tcccgtcctc gtcaacggcc aagccggcgt
     1621 catcatcgag atgaacggcg aaccgttcag cgtcatggcc ttcaccgtca ccgacgggat
     1681 catcgtcgcc atcgacatcc tcgccgaccc ggccaggctc ggtggagctg ggatcgaccc
     1741 gcggtgagtc agcgggggaa gttcgccgac agggacaggc agaaggggtg tccggccgga
     1801 tcgaggagca cgaggtggct ctcgggttcg ggttgcgatg gtgcccgcac ggcaccgagg
     1861 gagaccgctc gacgctcggc gtcagccaga tcgtcgacct cgaggtcgaa gtggccctgc
     1921 ttcggcaccg catcggtcgg ccaggtgggc gcgacgtagt cgtcgacccg catcgcggtc
     1981 agcaggacgt gatcgagctt gaccaccccg atgtcatccc tggtgacgat gacctcgccg
     2041 cccagcagat cggcccagaa ggcgctcagc ggtcctggat cagcgcagtc cagcgagacg
     2101 gcaccgagcc ggatcttcga cacccggacg gccggagcta ggccgccttc tcgaggatgt
     2161 ggacgccgca ggcggagccg aggccgatga catgggccag gcccaccttg gcgccttcga
     2221 tctgacggtc gccggcctcc ccgcggaggt ggtggcacac ctcccagatg ttggcgatgc
     2281 cggtggccgc gatcgggtgg cccttggact ccaggccgcc ggacacgttc accggggtcg
     2341 acccgtcccg ccaggtggcg ccggactcga agaacggcac cgcctcaccc tccttgcaca
     2401 gcatgaggtt gtcgtagtgg accagctcgg cggtggcgaa gcagtcgtgg agctcgacca
     2461 ggtcgaggtc ttcgggagcg accccggcct gctcgtaggc ctgagtggcg gcccggcgag
     2521 tgagggtgtt gacgtcgggg aggatctggc acgcctcctc ccacgggtcc gacgtgagga
     2581 cggacgcgga gaccttcacg gcccggcgct tctggtcggg gtcgagggtc tggagcttgg
     2641 cgtcgga
//
LOCUS       DASTFV010000537         3093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1984635, whole genome shotgun sequence.
ACCESSION   DASTFV010000537 DASTFV010000000
VERSION     DASTFV010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1984635"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VFP02_09855"
     CDS             complement(<1..777)
                     /locus_tag="VFP02_09855"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET9543377.1"
                     /translation="MQLGMIGLGRMGANMVRRLVRDGHECVVYDVSPDAVAALAAEAD
                     GITGAGSLADVAAAMPAPRSVWLMVPAGLVDQVIADVATHLEPGDTIIDGGNSNHKDD
                     IRRAAELSARGLHYVDVGVSGGVWGLENGYALMIGGEDEPVGRLESVFRSLAPGTGTV
                     LARTVGREGEPTTAEEGFLHCGPAGAGHYVKMVHNGIEYGLMAAYAEGLNILASAGIG
                     LSDQERNAETAPMRNPEEYQYQFDLAEVVEVWRRGTVIRSW"
     gene            1027..2646
                     /gene="pgi"
                     /locus_tag="VFP02_09860"
     CDS             1027..2646
                     /gene="pgi"
                     /locus_tag="VFP02_09860"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET9543378.1"
                     /translation="MATPVDQSPEWAALVDHRSALGDAHLRDLFADDPTRGTDFVVEV
                     GNLYLDYSKHRITRQTVGLLTDLARAAEVERLRDAMFAGEHINLTEDRAVLHTALRAP
                     SGTVIEDAGHDVVPDVHDVLDQMGRFAAAVRSGERRGFTGERLTNVVNIGIGGSDLGP
                     AMAAIALDAFVDRDLDLRFVSNVDGADLDLELRGLEPARTLVVVASKTFTTLETMTNA
                     RSARSWLVDALGEDAVEHHFVALSTNLAAVSAFGIDLANTFGFWDWVGGRYSLDSAIG
                     LSLMLGIGPDAFADLLAGFHLVDEHFRSAPLEANAPALLGLLGVWYSNFWDAQTHAVL
                     PYSQELARFPAYLQQLDMESNGKSVTRQGDPVGWDTGPVVWGEPGTNGQHAFFQLLHQ
                     GTRLVPADLIGFSRPNHPHVHHHDLLMANLFAQAEALAFGRSADEVRAAGVDEALVPH
                     RTFAGNRPTTTILADRLTPSVLGQLVALYEHKVFTQGAIWDVNSFDQWGVELGKSLAE
                     TIIPEVQAVADPELAHDTSTNALIRRYRSRR"
     gene            complement(2657..>3093)
                     /gene="rpiA"
                     /locus_tag="VFP02_09865"
     CDS             complement(2657..>3093)
                     /gene="rpiA"
                     /locus_tag="VFP02_09865"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HET9543379.1"
                     /translation="IDGADEIDAALNLTKGGGGALTREKIVAAMAERFVVVVDRSKVV
                     PLLGPFGTPIEVLSFAPAVVADRLRALGAASVERRDGLSDNGGVLLDAHFGEIANPTT
                     LAAELSAVPGIVEHGIFLADLVSTVVVADADGSIEILGQPVD"
BASE COUNT          461 a         1205 c          935 g          492 t
ORIGIN      
        1 ccacgaccga atgaccgtgc cccgtcgcca gacctccacg acctcggcca agtcgaactg
       61 gtactggtac tcctccgggt ttcgcatcgg cgccgtttca gcattgcgct cctggtcgga
      121 gaggccgatg ccagccgaag ccaggatgtt gagcccctcg gcgtaggcgg ccatcaggcc
      181 gtactcgatc ccgttgtgga ccatcttcac gtagtgaccg gcaccggcgg gcccacagtg
      241 caggaatccc tcttcggccg ttgtcggctc accttctcgc ccgaccgtcc gggccaggac
      301 ggtgcccgtc cccggcgcca gcgaccggaa cactgactcg aggcgtccca cgggctcgtc
      361 ctcgcccccg atcatcaggg cataaccgtt ctccaggccc caaacgcctc cgctcacgcc
      421 aacgtcgaca tagtgcaggc cccgcgccga gagttccgcc gcccgccgga tgtcgtcctt
      481 gtggttggag ttccctccgt cgatgatcgt gtcgccgggc tcgaggtggg tggccacgtc
      541 ggcgatcacc tggtcgacca ggcccgccgg caccatgagc cacaccgatc ggggagctgg
      601 cattgctgcc gccacgtcgg ccaacgagcc ggcgccggtg atgccgtccg cttccgccgc
      661 cagcgcggcc accgcgtccg ggctgacgtc gtacacgacg cactcgtgtc cgtcccgcac
      721 cagtcgccgg accatgttgg cccccattcg gcccagtccg atcatcccga gttgcatggc
      781 gcctccttca ggcccaccgt aggccgcccg ccccggaccg aacctcggcc tggcccggcc
      841 gcgagccgcc cgaccgacac cggaccgcac gaatccgcag tggccgttgc tcatcagtcg
      901 gggtcgagtg actgcgttgc cgaccggggc cccgactgct gtcgtcggcc tccgtgccgg
      961 ccgttccatc gactgctccg gccgccctcc gtcagacccg ctcaccccga gtcgtagcct
     1021 cgcctcatgg caacgcccgt cgaccagtct ccggaatggg cagccctcgt cgaccaccgc
     1081 tccgccctgg gcgacgccca cctccgggac ctcttcgccg acgaccccac ccggggcacc
     1141 gacttcgtcg tcgaggtcgg gaacctctac ctcgactact cgaagcaccg gatcacccga
     1201 cagacggtcg gacttctcac cgacctggcc cgagccgccg aggtcgaacg ccttcgggac
     1261 gccatgttcg ccggcgagca catcaacctc accgaggacc gggccgtcct ccacaccgcc
     1321 cttcgggcgc cgtcgggcac ggtcatcgaa gacgcgggcc acgacgtcgt acccgatgtg
     1381 cacgatgtcc tcgaccagat gggacgcttc gccgccgccg tgcgttccgg ggagcgccga
     1441 ggcttcacgg gggaacggct gaccaacgtc gtcaacatcg gcatcggtgg ctccgacctc
     1501 ggcccggcca tggccgccat cgcactcgac gccttcgtcg accgggacct cgacctccgc
     1561 ttcgtgtcca acgtggacgg agccgacctc gacctcgagt tgcggggtct cgaacccgcc
     1621 cgcaccctcg ttgtcgtcgc ctccaagacc ttcaccaccc tcgagaccat gaccaacgct
     1681 cgctcggccc ggtcctggct cgtcgacgca ctgggcgagg acgccgtcga gcaccacttc
     1741 gtcgccctgt ccaccaacct cgccgcggtc agcgccttcg gcatcgacct ggccaacacc
     1801 ttcggcttct gggactgggt cggcggtcgt tactcgctcg actcggcgat cggtctctcc
     1861 ctcatgctcg gcatcggccc cgacgccttc gccgacctac ttgccggctt ccacctggtc
     1921 gacgagcact tccgctcggc gccgctggag gccaacgccc ccgcgcttct gggccttctc
     1981 ggcgtgtggt actcgaactt ctgggacgcc cagacccacg ctgtccttcc ctacagccag
     2041 gagttggccc gcttccccgc ctacctccag cagctcgaca tggagagcaa cggcaagtcg
     2101 gtcacccggc agggcgaccc cgtgggctgg gacaccggtc ccgtcgtctg gggcgagccc
     2161 ggcaccaacg gccaacacgc gttcttccag ctcctccatc agggcacccg gctcgtccct
     2221 gccgatctca tcggcttcag ccggccgaac cacccgcatg ttcaccatca cgatctgctg
     2281 atggccaacc tgttcgccca ggccgaggcc ctggcgttcg gccggtccgc cgacgaggtg
     2341 cgggcggcgg gtgtcgacga ggccttggtg ccgcatcgga ccttcgccgg caaccgaccg
     2401 acgaccacga tcctggccga tcgcctcacc ccgtccgtcc tgggccagct cgtggccctc
     2461 tacgagcaca aggtcttcac gcaaggggcg atctgggacg tgaactcctt cgaccagtgg
     2521 ggcgtcgaac tgggcaagtc cctcgccgag acgatcatcc cagaggtcca ggcggtcgcc
     2581 gacccggagc tggcccacga caccagcacg aatgccctga tccgtcgcta ccgctcccgg
     2641 cgctgaggtc gacgcctcag tcgactggtt gcccgaggat ctcgatcgac ccgtcggcat
     2701 cggccacaac gacggtcgag accaggtcgg ccaggaagat cccgtgctcg acgatgccag
     2761 gtacggcgga caactcggcg gcgagggtgg ttggattcgc gatctcgccg aagtgggcgt
     2821 cgagcagcac accgccgttg tcgctcagcc cgtcccgtcg ctcgacggac gccgcaccga
     2881 gggcccggag ccggtcggcc accaccgcag gggcgaagga cagcacttcg atcggcgtgc
     2941 cgaacggacc gagcagcggt acgaccttcg atcgatcgac tacgacgacg aagcgttccg
     3001 ccatcgcggc cacgatcttc tcgcgggtca gggccccccc gccgcctttg gtcaggttga
     3061 gcgcggcgtc gatctcgtcg gccccgtcga tcg
//
LOCUS       DASTFV010000538         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_1993792, whole genome shotgun sequence.
ACCESSION   DASTFV010000538 DASTFV010000000
VERSION     DASTFV010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993792"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="VFP02_09870"
     CDS             complement(<1..1535)
                     /locus_tag="VFP02_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9543380.1"
                     /translation="MTGPPVSAPWRAAHVLALSSLAVTQPVLGVLGDNPTFFVAHHAD
                     RGEVLLVSGLVAFAVPVALIAVELLVGLVRRAWAWPVHLVVMGLLAAVVAAGVLDDLV
                     AAVLDDLPYVSGPVTVLGAAGAGYAFTRAYVRRDLLRSVVSVLAFAPAVFLAFFAFAS
                     PTRDLLFPEAVAAADVTVTGAQPPIFVVVFDELPLASVLTVDGDTIDAERFPNLARLA
                     DDGIWYPNATSVAAYTHEAVPAILTGNRVHDDNVPPTASGHPDNLFTFLAQDYDIAAY
                     EQLTELCPSDLCEESSSGGARAAGDVLYEDLAVVAGHVTLPGLLEGWLPTINDTWANF
                     GHDPVALDLEAERMTNDRHAFVDALGDYDRVGQFRAALDDIQARRAPRLTFIHTVFPH
                     VPWTYHADGSSYPNPGNTGLSDNEWTTSSGADLGLQRHLLQAERADLLLGEFLDRLEA
                     EGLYDDALVVFTADHGASFTVGTHRRVPEEDSLAGLMPVPLVVKPPSTTPVADHRDDR
                     TAET"
     gene            complement(1532..>2371)
                     /locus_tag="VFP02_09875"
     CDS             complement(1532..>2371)
                     /locus_tag="VFP02_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9543381.1"
                     /translation="YVDTLVGQTVARLQESPLWDDAVLAVVADHGASFQDGQARRVLT
                     EHNAHEIMWSTLFIRAPGLASGTTDVNMSSTDLLPTLADLMGTKVPWPTSGASILDAD
                     DRSAASAAGDKAYYRFSNRWFYEPDAVLQIDGPSNLARLLEDGPRAFAAEDPVGSLYR
                     ATPPGDLYDAALDDLEVGAPLVVTASVEGLDGIEGGAEDVRHAYVGGGLTGGDVGDDA
                     WVVAVADGVVSGMSPLFEEERHERGFALLLDADRVPADGVDLELLLWEDGALRPLIVR
                     GEG"
BASE COUNT          355 a          793 c          920 g          303 t
ORIGIN      
        1 gtctcggcgg tccggtcgtc ccgatggtcg gccacggggg tcgtggacgg cggcttgacc
       61 acgaggggca cgggcatgag gccggcgagg ctgtcctcct ccggcacgcg ccggtgggtg
      121 ccgacggtga acgacgcccc gtggtcggcg gtgaacacga ccaacgcgtc gtcgtacagg
      181 ccctcggcct cgagtcggtc gaggaactcg ccgagcagca ggtcggcccg ctccgcctgg
      241 agcaggtggc gctgcaggcc gaggtccgcg cccgagctgg tggtccattc gttgtcgctc
      301 agcccggtgt tgcccgggtt cgggtaggac gatccgtcgg cgtgataggt ccacgggacg
      361 tgggggaaca cggtgtggat gaacgtgagc cggggcgccc gccgggcctg gatgtcatcg
      421 agcgccgccc ggaactgacc gacccggtcg tagtcgccga gcgcgtcgac gaaggcgtgc
      481 cggtcgttgg tcatccgctc ggcctcgaga tcgagggcca cggggtcgtg accgaagttg
      541 gcccacgtgt cgttgatcgt cggcagccag ccctcgagga ggccgggcag cgtgacgtgg
      601 ccggcgacga cggccaggtc ctcgtagagg acgtcgcccg cggcgcgagc accgccggac
      661 gaggactcct cacacagatc cgacggacac agctcggtga gctgttcgta ggccgcgatg
      721 tcgtagtcct gggccaggaa ggtgaagagg ttgtcggggt gaccgctggc cgtcggcggc
      781 acgttgtcgt cgtgcacccg gttgccggtg aggatggcgg ggacagcctc gtgggtgtag
      841 gcggccacgg aggtggcgtt ggggtaccag atgccgtcgt cggccaaccg ggccaggttc
      901 gggaaacgct ccgcgtcgat cgtgtcgccg tccaccgtga gcactgacgc cagcgggagc
      961 tcgtcgaaga ccacgacgaa gatcggcggc tgcgctccgg tgaccgtgac gtcggcggcg
     1021 gccaccgcct ccgggaagag aaggtcgcga gtgggggacg cgaaggcgaa gaaggcgagg
     1081 aagacggccg gcgcgaaggc gagcaccgag accaccgacc gcagcaggtc acgccggacg
     1141 taggcccggg tgaacgcgta gccggccccc gcggcaccga ggacggtgac ggggccggac
     1201 acgtacggca ggtcgtcgag caccgcggcg acgaggtcgt ccagcacccc ggccgccacg
     1261 acggcggcga gcaggcccat cacgaccagg tggacgggcc aggcccacgc ccggcgcacc
     1321 aacccgacga ggagctcgac ggcgatgagg gccacgggga cggcgaaggc gaccaacccg
     1381 ctgaccagga ggacctcgcc gcgatcggcg tggtgggcca cgaagaaggt cgggttgtcc
     1441 ccgagcaccc ccagcaccgg ttgggtcacc gccaggctgc tgagcgcgag gacgtgggcc
     1501 gctcgccacg gcgccgagac gggcgggccg gtcacccctc gccccgcacg atgaggggtc
     1561 ggagcgcgcc gtcctcccac agcagcaact cgaggtcgac accgtcggcg ggtacccggt
     1621 cagcgtccag gaggagggcg aaccctcgct cgtggcgttc ctcctcgaag agcggcgaca
     1681 tcccgctgac cacgccgtcg gccacggcca ccacccaggc gtcgtcgccg acgtcgcctc
     1741 ccgtgagccc gccgcccacg taggcgtggc ggacgtcctc ggcgccgcct tcgatgccgt
     1801 ccaggccctc gaccgaggcg gtcacgacga ggggcgcgcc gacctcgagg tcgtcgagtg
     1861 cggcgtcgta gaggtcgccg ggcggggtcg cacggtagag cgagcccacg gggtcctcgg
     1921 cggcgaaggc ccggggtccg tcctcgagca gccgggccag gttgctgggc ccgtcgatct
     1981 gcaggacggc gtcgggttcg tagaaccacc ggttggagaa gcggtagtac gccttgtccc
     2041 ccgccgcgct ggccgccgag cggtcgtcgg cgtccaggat cgaggccccc gacgtcggcc
     2101 agggcacctt ggtgcccatg aggtcggcca gcgtgggcag caggtccgtc gaggacatgt
     2161 tcacgtcggt ggtcccggac gcgagcccgg gggcccggat gaacagcgtc gaccacatga
     2221 tctcgtgggc gttgtgctcg gtcagcaccc gccgggcctg accgtcctgg aagctggccc
     2281 cgtggtcggc gacgacggcg aggacggcgt cgtcccacag ggggctctcc tggaggcgag
     2341 cgacggtctg gcccacgagg gtgtcgacgt a
//
LOCUS       DASTFV010000539         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2005155, whole genome shotgun sequence.
ACCESSION   DASTFV010000539 DASTFV010000000
VERSION     DASTFV010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2005155"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..>3060
                     /gene="ileS"
                     /locus_tag="VFP02_09880"
     CDS             48..>3060
                     /gene="ileS"
                     /locus_tag="VFP02_09880"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET9543382.1"
                     /translation="MTDNAETSGPYPDVTPNADYPAVERGVLERWAADHTFERSVEQR
                     PADDEYVFYDGPPFANGLPHYGHFLTGYVKDVVPRYQTMRGRRVDRRFGWDCHGLPAE
                     IEAERQLGVSGRRQIQDYGIERFNDHCRTSVQQYTADWEQYVTRMARWVDFENDYKTM
                     DVSYMESVIWAFKRLWDKGLVYKAYRVMPYSWGAETPLSNFEIRLDDATRPRQDPALT
                     VAVRLLPIDGDPGPLSILIWTTTPWTLPSNLAVAVGGDITYVVLKDPDSEEHYVLGEA
                     TLDKYEGQVGGYDRVATLTGQDLAERRYEPIFPYFADHPNSFRVLIGDYVDTEEGTGV
                     VHLAPGFGEEDQRVGEAAGIELVVPVDDAGRFTAEVPDWEGENVFDANPEVIKALKDR
                     GVVVRHDSYEHNYPHCWRTDTPIIYKAVDSWFVEVTAFRDRLVELNQEINWIPDHVRD
                     GAFGRWLEGARDWSISRNRFWGSPIPVWQSDDPAYPRTDVYGSLDEIEADFGVRPDDL
                     HRPTVDDLVRPNPDDPTGKSMMRRVPEVLDCWFDSGSMPYAQVHYPFENQAWFEDHFP
                     ADFIVEYIAQTRGWFYTLHVLSTALFDRPPFQNVICHGILLAEGGRKISKRLRNYPEP
                     DLVFNTYGADALRWYFMSSPILRGLDLQIDKEGTKIAEVVRTVLHPLWSAYHFFTLYA
                     NVDGHRATFRTDATDTLDRYILAKTHDLVARTTEAMDAYDIAGACAEVQSFSEVLNNW
                     YIRRSRDRFWAASSDVTAADKADAYDTLFSVLVTLCRVAAPLLPLISDEIHRGLVGGD
                     SVHLDDWPSVDDLPADDELVAAMDRVRQVASTTLSLRDAERLRVRMPLPKLTVAGPGA
                     AELEGFLDLLGDEVNVKEVVLEDDPSTLATFRLKPNGKVLGPLLGGAVQEVMKAARDG
                     EWASGDDGTVVVAGHTLAPGQFELAVEAGESGATAALPGNDTVIRLDTELTDDLRAEG
                     MARDLARLVQQARKDRGLAVTDRITLSVRLPE"
BASE COUNT          490 a         1139 c          987 g          444 t
ORIGIN      
        1 ccggccagcc ctaggctggc ggctccccct gagcacagag gccgcgagtg accgacaacg
       61 ccgagacgtc cggtccgtac ccggacgtca cgcccaacgc cgactacccc gccgtcgagc
      121 gcggcgtgct ggagcgctgg gcggccgacc acaccttcga gcgctcggtc gagcagcgcc
      181 cggccgacga cgagtacgtc ttctacgacg gtcccccgtt cgccaacggc ctcccccact
      241 acggccactt cctgacgggc tacgtgaagg atgtcgtccc ccgctaccag acgatgcggg
      301 gccgccgggt cgaccgtcgc ttcggctggg actgccacgg cctgccggcc gagatcgagg
      361 ccgagcgcca gctcggggtg tccggacggc gccagatcca ggactacggc atcgagcgct
      421 tcaacgacca ctgccggacg tcggtgcagc agtacacggc ggactgggag cagtacgtca
      481 cccgcatggc ccgctgggtc gacttcgaga acgactacaa gacgatggac gtctcctaca
      541 tggagagcgt catctgggcg ttcaagcggc tgtgggacaa gggcctcgtg tacaaggcgt
      601 accgggtcat gccgtattcg tggggtgcgg agacgccact gtcgaacttc gagatccgcc
      661 tcgacgacgc cacccgcccc cgccaggacc cggccctcac cgtcgccgtc cgcctcctgc
      721 cgatcgacgg cgaccccggg ccgctgtcga ttctcatctg gaccaccaca ccctggacgc
      781 tgccgtccaa cctggccgtc gcggtcggcg gggacatcac ctacgtcgtg ctgaaggacc
      841 ccgactccga ggagcactac gtcctgggcg aggccaccct cgacaagtac gagggccagg
      901 tcggcggcta cgaccgagtg gccacgctca ccggccagga cctggccgag cgtcgctacg
      961 agccgatctt cccgtacttc gccgaccacc cgaacagctt ccgggtcctc atcggcgact
     1021 acgtcgacac cgaggagggc accggggtcg tccacctggc ccccgggttc ggtgaagagg
     1081 accagcgggt cggtgaggcc gccggcatcg agctggtcgt cccggtcgac gacgccggcc
     1141 gcttcaccgc cgaggtcccc gactgggagg gggagaacgt cttcgacgcc aaccccgagg
     1201 tcatcaaggc cctgaaggac cggggcgtcg tcgtccgcca cgactcctac gagcacaact
     1261 acccccactg ctggcggacc gacacgccga tcatctacaa ggccgtcgac tcctggttcg
     1321 tggaggtgac cgcgttccgg gaccgtctgg tcgagctgaa ccaggagatc aactggatcc
     1381 ccgaccacgt cagggacggg gccttcggcc gctggttgga gggggcccgg gactggtcca
     1441 tcagccgcaa ccgcttctgg ggctcgccca tcccggtgtg gcagagcgac gacccggcct
     1501 acccccgcac ggacgtctac gggagcctcg acgagatcga ggccgacttc ggtgtccgcc
     1561 ccgacgacct gcaccgaccc accgtcgacg acctggtccg ccccaacccc gacgacccga
     1621 ccgggaagtc gatgatgcgc cgcgtccccg aggtcctcga ctgctggttc gactcgggct
     1681 ccatgcccta cgcccaggtc cactacccct tcgagaacca ggcgtggttc gaggaccact
     1741 tccccgccga cttcatcgtg gagtacatcg cccagactcg gggctggttc tacacactgc
     1801 atgtgctgtc gacggcgctg ttcgaccggc caccgttcca gaacgtgatc tgccacggga
     1861 tcctgctggc cgagggcggc cgcaagatct ccaagcgcct gcgcaactac cccgagcccg
     1921 acctcgtgtt caacacctac ggggccgacg ccctgcgttg gtacttcatg tcctcgccga
     1981 tcctgcgagg cctcgacctg cagatcgaca aggagggcac caagatcgcc gaggtggtgc
     2041 gcacggtgct gcaccccctc tggtcggcgt accacttctt caccctctac gcgaacgtcg
     2101 acggtcaccg ggcgaccttc cgtaccgacg ccactgacac cctcgaccgg tacatcctgg
     2161 ccaagaccca cgacctggtc gcccgcacca ccgaggccat ggacgcctac gacatcgccg
     2221 gggcctgcgc cgaggtccag tccttcagcg aggtgctgaa caactggtac atccgccgga
     2281 gccgggaccg cttctgggcc gcgtcctccg acgtcacggc ggccgacaag gccgatgcct
     2341 acgacacgct cttctccgtg ctggtcaccc tctgccgggt ggccgctccc ctgctgcccc
     2401 tgatcagcga cgagatccac cggggcctgg tcggcggcga cagcgtccac ctggacgact
     2461 ggccgtcggt cgacgacctc cccgccgacg acgagctggt ggcggccatg gaccgggtgc
     2521 gccaggtcgc ctccaccacg ctgtcgctcc gggacgccga gcggctgcgg gtccgcatgc
     2581 cgttgccgaa gctgaccgtg gccggccccg gcgccgccga gctcgagggc ttcctcgacc
     2641 tgttgggcga cgaagtcaac gtgaaggagg tcgtgctcga ggacgacccc tcgacgctgg
     2701 ccacgttccg tctgaagccg aacggcaagg tgctgggccc cctcctcggc ggggctgtcc
     2761 aggaggtcat gaaggcggcg cgtgacggcg agtgggcatc aggagacgac ggcaccgtcg
     2821 tggtcgccgg gcacacgctg gcgcccggcc agttcgagct ggcggtcgaa gcgggtgagt
     2881 ccggggccac ggccgcgttg cccggcaacg acaccgtcat ccggctcgac accgagctga
     2941 ccgacgacct ccgggccgag ggcatggccc gggacctggc ccgtctcgtc cagcaggccc
     3001 gcaaggaccg cggcctggcc gtgaccgacc gcatcaccct gtcggtccgg ctcccggagc
//
LOCUS       DASTFV010000540         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2006445, whole genome shotgun sequence.
ACCESSION   DASTFV010000540 DASTFV010000000
VERSION     DASTFV010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2006445"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VFP02_09885"
     CDS             complement(<1..619)
                     /locus_tag="VFP02_09885"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET9543383.1"
                     /translation="MTPDRNPHAGLPITDDDATIAAALEDVSIPTLMLSMVHMTGDAE
                     IIRGELRPQGLFLNEVQGYMSEEDKAEVRRRALDVIRVYRDGGSVPAPPPDPELIREM
                     MSWLVCQDVPEAYVPMLLEEMELDGVDAREVALSVDQGDRADFPVVVVGCGQSGLLAG
                     IRLQEAGIPYTIIEKNAGVGGTWYENTYPGCRVDVGNHFYCYSFEP"
     gene            664..1161
                     /locus_tag="VFP02_09890"
     CDS             664..1161
                     /locus_tag="VFP02_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET9543384.1"
                     /translation="MGTNERSKIAMTDEEVTTFIERSRTCTMATVGPTGQPHLVAMWY
                     AVVDGQVWFETKAKSQKVQNLKRDDRITVSIEDGLTYDTLRGVAIEGRATILDDRDAL
                     WKVGVSVWERYNGPYTEEMAPFVEVMLNKRVAVRVDVDRIRTWDHRKLGMAPTELGGS
                     TAEFL"
     gene            1215..>2614
                     /locus_tag="VFP02_09895"
     CDS             1215..>2614
                     /locus_tag="VFP02_09895"
                     /inference="COORDINATES: protein motif:HMM:NF023500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HET9543385.1"
                     /translation="MGVIKPALPDLDLDDWAARPEADRVRLMCESWAVQGFGAPAVAY
                     FFYVAKIAVYVCLWVVLVSRAPDVQGWSDIGDWWATPIAFEKAILWTMLFEVLGLGCG
                     SGPLTGRYVPPIVAFTHFLRPSTLRLPAFPDRVPLTAGHRRTVVDALLYAALLGLCLR
                     ALLSSEAVLDEGLLVPIIAVLAVLGLRDKTIFLAARAEHYWTMLIVFALAGDDVTVLL
                     AGAMVVQLAIWWGAASSKLNHHFPNVVTIMVSNSPVLRVPALKKAMYRHYPDDLRASG
                     LAAALAHGGTVIEFAFPLLLAVSSGGAPTTVGLAVMLSFHLFITSCFPMGVPIEWNVL
                     FVYSMFVLFGHYAELSPLDVFDDPLLAVALAAGILVGPVFGGLRPDKVSFLPAMRYYA
                     GNWAASLWLFRRGSDEVLDQKVTKTAKTIPKQLALLYDDDTIRAVQGKLRAFRSMHLH
                     GRALNAVLPTVVDDLEA"
BASE COUNT          371 a          921 c          888 g          434 t
ORIGIN      
        1 agggctcgaa ggagtagcag tagaagtggt tgccgacatc gacccggcag ccggggtagg
       61 tgttctcgta ccaggtgccc ccgaccccgg cgttcttctc gatgatcgtg tacgggatgc
      121 ccgcctcctg cagccggatc ccggccagga gccccgactg cccgcaaccg acgaccacga
      181 ccgggaagtc ggcccgatcg ccctggtcga cgctgagggc gacctcgcgg gcatcgaccc
      241 cgtccagctc catctcctcg aggagcatcg ggacgtacgc ctcggggacg tcctggcaga
      301 ccagccagct catcatctcc cggatcagtt cggggtcggg tgggggtgcc ggcaccgacc
      361 cgccgtcccg gtacacccgg atgacgtcga gggcccgacg gcggacctcg gccttgtcct
      421 cctcgctcat gtagccctgg acctcgttga ggaagaggcc ctggggccgg agctcgcccc
      481 ggatgatctc ggcgtccccg gtcatgtgga ccatcgacag catcagggtc gggatgctca
      541 cgtcctcgag ggcggcggcg atcgtggcgt cgtcgtcggt gatgggcaga ccggcgtggg
      601 ggttcctgtc gggcgtcacg gtctgtcacc attccacgcg ggacgccgag cagaggaacg
      661 ccgatgggaa ccaacgagcg cagcaagatc gccatgaccg acgaggaggt gaccaccttc
      721 atcgagcgga gccgcacctg caccatggcg acggtcggcc ccaccggcca gccccatctc
      781 gtggcgatgt ggtacgccgt cgtcgacggc caggtctggt tcgagaccaa ggccaagtcc
      841 cagaaggtcc agaacctgaa gcgagacgac cgcatcacgg tctcgatcga ggacggcctg
      901 acctacgaca cgctgcgggg tgtcgccatc gaaggtcggg ccacgatcct cgacgaccgc
      961 gacgccctct ggaaggtcgg agtcagcgtg tgggagcgct acaacgggcc gtacaccgag
     1021 gagatggcgc ccttcgtcga ggtcatgctc aacaagcggg tggcggtccg ggtcgacgtg
     1081 gaccggatcc gcacgtggga ccaccgcaag ctgggcatgg cccccaccga gctgggcggc
     1141 tccaccgccg agttcctgtg agctgatccg gccgtccgcc gggtgacgaa ccccgcggcc
     1201 gagagggggt ggggatgggc gtgatcaagc cggcgctgcc ggacctggac ctggacgact
     1261 gggcggcccg gcccgaggcc gatcgggttc gtctcatgtg cgagtcctgg gccgtgcagg
     1321 gcttcggggc gccggccgtc gcctacttct tctacgtcgc caagatcgcg gtgtacgtct
     1381 gcctgtgggt cgtcctcgtc agccgggcgc cggacgtcca gggatggagc gacatcgggg
     1441 actggtgggc cacgcccatc gccttcgaga aggcgatcct ctggacgatg ctgttcgagg
     1501 tcctgggcct cggctgtggc agtgggccct tgaccggccg gtacgtcccg ccgatcgtgg
     1561 ccttcaccca cttcctgcgc cccagcaccc tgcgccttcc ggccttcccg gaccgggtgc
     1621 cgctgaccgc cggccaccgg cgcaccgtgg tcgatgctct gctctacgcc gccctcctcg
     1681 ggctgtgcct ccgggcgctg ctctcctccg aggccgtgct ggacgagggc ctgctcgtcc
     1741 cgatcatcgc cgtcctcgcc gtgctcggac tgcgggacaa gacgatcttc ctggccgccc
     1801 gggccgagca ctactggacg atgctgatcg tcttcgcgct ggcgggcgac gacgtcacgg
     1861 tcctgctggc cggggccatg gtcgtccagc tggccatctg gtggggggcg gccagctcca
     1921 agctcaacca ccacttcccc aacgtggtca cgatcatggt cagcaactcg ccggtcctcc
     1981 gggtgccggc gctgaagaag gccatgtacc gccactaccc cgacgacctc cgggcgtcgg
     2041 gcctcgccgc ggccctggcc cacggcggca ccgtgatcga gttcgccttc cccctcctgc
     2101 tggccgtgtc ctccggcggc gcgcccacga ccgtcggcct ggccgtgatg ttgtcgttcc
     2161 acctcttcat cacgtcgtgc ttcccgatgg gtgtgcccat cgagtggaac gtcctcttcg
     2221 tctattcgat gttcgtgctg ttcgggcact acgccgagct cagcccgttg gacgtgttcg
     2281 acgacccgtt gttggctgtg gccctggcgg cgggcatcct ggtcggcccc gtcttcggcg
     2341 gcctccggcc ggacaaggtc tcgttcctgc ccgccatgcg ctactacgcg ggcaactggg
     2401 cggccagcct ctggctcttc cggcgcggga gcgacgaggt cctggaccag aaggtcacca
     2461 agacggcgaa gacgatcccg aagcagctgg ccctgctcta cgacgacgac accatccggg
     2521 cggtgcaggg caagctgcgg gccttccgct cgatgcacct ccacgggcgg gccctcaacg
     2581 ccgtgctgcc gacggtggtc gacgacctcg aggc
//
LOCUS       DASTFV010000541         3533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2012555, whole genome shotgun sequence.
ACCESSION   DASTFV010000541 DASTFV010000000
VERSION     DASTFV010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2012555"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VFP02_09900"
     CDS             <1..418
                     /locus_tag="VFP02_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543386.1"
                     /translation="GTEVVEADIFLLTDEKPSLLGPEALRLERSEPASDLLLDDLRSD
                     RHSAWVPEEAWLSYLRIDAPAAALDGDLAIDVGGNHPSRVAAGLARGELAAGPTIATG
                     SDDPWLNWVLAGLGAAVVTGGLLVLLRTGDQAGRAS"
     gene            complement(397..1014)
                     /locus_tag="VFP02_09905"
     CDS             complement(397..1014)
                     /locus_tag="VFP02_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9543387.1"
                     /translation="MLPPHTRLGPRFVEAVAWVTELHADQPGKGGRDVPYVSHLLAVA
                     ALTLEDGGDETDAVIALCHDAVEDQGGDVALAEIRRRFGQEVADAVALLSDGRGEPPV
                     PWRERKERLLGQLRAPEATERVLRVAAADKLDNARTLLAGLDREGSRAWEHREAAPED
                     YLWFYGSVGELLAERYPGSANVALLERIVAELAAWVPPDQLARPA"
     gene            1079..2290
                     /locus_tag="VFP02_09910"
     CDS             1079..2290
                     /locus_tag="VFP02_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9543388.1"
                     /translation="MADPAPPALRRDDAMPPWIPRAILWFFGTGLAIVVAWWLVVRLR
                     GLLVMLLVALFLSFALEPAVNWMAERGWRRGAATLTVMFVLLIAGAIFVWAMGAVLVN
                     QVTDFVDEAPRYIEDAEDWFNDTFDADVDFEDLIAEFQEGGSAQEFAAKLGGNIVSAG
                     ATVVAVLFQMLTIALFTFYLVADGPRLRRAILSVLPPDRQVEVLRVWEIAIDKTGGYI
                     YSRGILAFFSTLVHWIAFAVIGIPFPLPLAIWVGLISQFVPTIGTYIAGALPIVIALI
                     DKPIDALWVLAVILVYQQIENYLLAPPIQAETMDIHPAVAFGGVIAGASILGPIGALL
                     ALPAAATLQAFVSTYVRRHEVIETPLTARPRARVDFLRPLRYLRRTKPESVASGDADA
                     DGDGTTSESIR"
     gene            2321..3112
                     /locus_tag="VFP02_09915"
     CDS             2321..3112
                     /locus_tag="VFP02_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543389.1"
                     /translation="MSKRWSVLFALVGVLIGSAPAALAAGGAGGSVTGGVDPSGVTIT
                     IVRWQGSDRVHATARGGGDDPTGCDWAIVPAPLGALPPADIPPYQPDAYLGLLTCDGV
                     GVEVIWVGPDNTVDLEAEARRLVEEYVAQVPVPRITVHANPRPSGLVGLESWFWATGY
                     DQRPIVDRIDALGFGVDVRIDPTPASWSFGDGSTATGDLGEAYPNPSSIRHGFTEHGT
                     RPVEAGFALVPRYRIDGTAWIELPSIPVTDALTYRVREAQAVITG"
     gene            complement(3125..3364)
                     /locus_tag="VFP02_09920"
     CDS             complement(3125..3364)
                     /locus_tag="VFP02_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543390.1"
                     /translation="MNATHVSPERGSTDPRSTRRLALVAAWVMAVAGAGLLLQALVSG
                     ARFELVVLALGLGAGAALIGLLVLTPGLSVTRDDD"
BASE COUNT          468 a         1298 c         1238 g          529 t
ORIGIN      
        1 gggaaccgag gtggtcgagg ccgacatctt cctgctgacc gacgagaagc cgtcactcct
       61 cgggcccgaa gcgctccgcc tcgagcgctc cgagcccgct tcggatctgc tcctcgacga
      121 cctgcgctcg gaccggcaca gcgcctgggt gcccgaagag gcgtggctga gctacctgcg
      181 catcgacgct ccggcggcgg ccctcgacgg tgacctggcc atcgacgtcg gcggcaacca
      241 cccgtcgcgg gtggccgcgg ggctggcgcg gggcgagctg gcggccgggc ccacgatcgc
      301 cacggggtcc gatgatccct ggctgaactg ggtcctggcc gggctgggcg ccgccgtggt
      361 gacgggcggg ctgctggtcc tgttgcggac cggggatcag gccggtcgag ccagctgatc
      421 gggcgggacc caggcggcca gctcggccac gatccgctcg agcagggcga cgttcgcgct
      481 gcccggatac cgctcggcca ggagctcccc gaccgacccg tagaaccaga ggtagtcctc
      541 gggggccgcc tcgcggtgct cccaggctcg gctcccttca cggtccaacc cggccaggag
      601 ggtccgggcg ttgtcgagtt tgtcggcggc cgccactcgc aggacgcgct cggtggcctc
      661 gggggcacgg agctggccga gcaggcgctc cttccgctcc cgccagggga ccgggggctc
      721 gcctcggccg tcggacagga gggccacggc gtcggccacc tcctggccga agcggcgtcg
      781 gatctccgcc agcgcgacgt ccccgccctg gtcctcgacg gcgtcgtggc aaagggcgat
      841 gacggcgtcg gtctcgtcgc ccccgtcctc gagggtgagc gcggcgaccg ccaggaggtg
      901 gctgacgtag gggacgtcgc ggccgccctt gcccggctga tcggcgtgga gctcggtgac
      961 ccaggcgacg gcctcgacga agcgggggcc gagacgggtg tgagggggga gcacaacaga
     1021 atttgtaccg cgcttgaccc tccggaggcc agcgcccggc accaggtacg gtcgggccat
     1081 ggccgacccg gcgcccccag cgctccggcg cgacgacgcc atgccgcctt ggatcccccg
     1141 agcgatcctg tggttcttcg gcaccggcct ggccatcgtc gtcgcctggt ggctggtggt
     1201 gcgcctccgg ggcctgctgg tgatgctcct cgtcgccctc ttcttgtcct tcgcgctgga
     1261 gccggcggtc aactggatgg ccgagcgggg gtggcgccgg ggggcggcga ccctcacggt
     1321 gatgttcgtc ctgctcatcg ccggcgccat cttcgtctgg gccatgggtg cggtcctggt
     1381 caaccaggtc accgacttcg tcgacgaggc cccccgctac atcgaggacg ccgaggactg
     1441 gttcaacgac accttcgacg cggacgtcga cttcgaggac ctgatcgccg agttccagga
     1501 gggcggctcg gcacaggagt tcgcggccaa gttggggggc aacatcgtca gcgcgggggc
     1561 gaccgtggtc gccgtgctgt tccagatgct caccatcgcc ctcttcacct tctacctggt
     1621 cgccgacggg ccacgcctgc gtcgggcgat cctctccgtg ctgccgccgg accgtcaggt
     1681 cgaggtcctg cgggtgtggg agatcgccat cgacaagacc ggtggctaca tctacagccg
     1741 gggcatcctc gccttcttct ccacgttggt gcactggatc gcgttcgccg tcatcggcat
     1801 ccccttcccg ctgcccctgg ccatatgggt gggcctcatc agccagttcg tgcccaccat
     1861 cgggacctac atcgccgggg ccctgccgat cgtcatcgcc ctgatcgaca agcccatcga
     1921 cgccctgtgg gtcctggccg tgatcctcgt gtaccagcag atcgagaact acctgctggc
     1981 cccgccgatc caggccgaga cgatggacat ccaccccgcc gtcgccttcg gcggggtcat
     2041 cgccggggcc agcatcctcg ggcccatcgg cgccctgctg gcccttcccg cggcggccac
     2101 gttgcaggcc ttcgtctcca cctacgtgcg gcgccacgag gtcatcgaga cccccctcac
     2161 cgcccgcccc cgggcccggg tcgacttcct ccgccccctg cgctacctgc gccgcaccaa
     2221 gccggagtcg gtggcgtcgg gcgacgccga tgccgacggc gatgggacaa catcagaaag
     2281 catccgttga cattgacata atctgaaata gattggcagg gtgtccaaga gatggagcgt
     2341 tctgttcgcc ctggtgggag tgctgatcgg ctcggcccct gcggccctgg cggccggtgg
     2401 ggccgggggc tcggtcaccg gcggggtgga cccgtcgggc gtgaccatca ccatcgtgcg
     2461 ctggcagggc agtgaccggg tccacgccac cgcccggggt gggggcgacg accccaccgg
     2521 gtgtgactgg gccatcgtcc cggctcccct gggtgccctg ccccccgccg acatcccgcc
     2581 ctaccagccc gacgcctacc tcggcctgct cacctgtgac ggcgtcgggg tggaggtcat
     2641 ctgggtcgga cccgacaaca cggtcgacct cgaggccgag gcccgccgcc tcgtcgagga
     2701 gtacgtggcc caggtcccgg tcccgcgcat cacggtccac gccaacccgc ggccctcggg
     2761 cctggtcggc ctcgagtcgt ggttctgggc caccggctac gaccagcgcc cgatcgtcga
     2821 ccgcatcgac gccctcggat tcggcgtcga cgtccgcatc gaccccaccc ccgcctcgtg
     2881 gtcgttcggt gacggctcga ccgccacggg tgacctcggt gaggcctatc cgaacccctc
     2941 ctcgatccgc cacgggttca ccgagcacgg cacccgcccc gtcgaggccg gcttcgccct
     3001 cgtcccccgc taccgcatcg acggcaccgc ctggatcgag ctcccgtcga tccccgtcac
     3061 cgacgccctg acctaccggg tgcgggaggc ccaggccgtg atcacgggct gagcccgggg
     3121 tggctcagtc gtcgtcgcgg gtgacggaga ggccgggggt gaggacgagc aggccgatga
     3181 gggcggcgcc ggctcccagg ccgagggcca ggaccaccaa ctcgaaacgg gcgccggaga
     3241 cgagggcctg gagcaggagg cccgccccgg cgacggccat gacccaggcg gccacgagcg
     3301 cgagccggcg ggtcgaacgg gggtccgtcg agccccgctc cggcgacacg tgggtggcgt
     3361 tcattacaaa caggttacaa catgggtcca gcggcgacag gggcatggcc ccgccgtccc
     3421 ggacctagcc cacgaatcgc ccgcagatgg gccacggctg gtcgccccga acctggaaga
     3481 gggccttggc ccgcatgtcc tgttcccagt acggggcggt ggagggccgc ccc
//
LOCUS       DASTFV010000542         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2014639, whole genome shotgun sequence.
ACCESSION   DASTFV010000542 DASTFV010000000
VERSION     DASTFV010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2014639"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1621)
                     /locus_tag="VFP02_09925"
     CDS             complement(149..1621)
                     /locus_tag="VFP02_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9543391.1"
                     /translation="MNVAGIIDGHPDDAVALVVGDRTVSYGELRREVAGLQGGLTELG
                     LDVGDRVGLMAGNTVEFVLTWLAAARGGFVAVPLNPGSPGPELARELVAVGARAVVCG
                     PEGSTVVADLDRAALPVLEHVIVGDDLADLVAHAPAETVEREAADLAVLMFTSGTAGA
                     PRAAMLTHGNLVANLEQVQASEGRRRGPDDVALGVIPLFHIFGLNVVLGGGLVAGSRI
                     VLLDRFDAEEALRAIVAHGVTDLTGPPAMWHGLAAAESASPDAVATVERAASGAAALG
                     PEIRKSVQERLGLALAEGYGLTETSPVVATGVGQDAPVGSVGRPLPGVEVRIIDADGE
                     DTFIGDAGEILVRGDNVFVGYWNDPEATTAVLTGDGWLHTGDIGFADESGFLFLSDRA
                     KDLIIVSGFNVFPAEVEDVLVSHPDVAEAAVVGVPNARTGEAVKAFVVLEEGAGIGLE
                     DLREHTHGHLARYKCPAEIELVDELPHGPSGKLLRRTLRG"
     gene            1722..>2132
                     /locus_tag="VFP02_09930"
     CDS             1722..>2132
                     /locus_tag="VFP02_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9543392.1"
                     /translation="MTAIRTDGLTKAFGEHRAVDGLTLDVAPGEVLALLGPNGAGKTT
                     TVRLLNGVLAPDAGAAQVLGMDPAVDGDEVRRRTGVLTENAGLDDRLTARENLVFTAR
                     IRGMGIDEAQSRTAEVLDQFAMADLADARVHGFST"
BASE COUNT          294 a          802 c          770 g          266 t
ORIGIN      
        1 cagcccatcc gcgtccgtcg cgccgcgcca tgcgctcagt cgagcggcag gcgccagacc
       61 gtggtgacgt cgaagccctg gtctcatggc gacagcgtca cggaccgttg ccggcgaggc
      121 gagggtgccg gcctccacat ccggagggct acccccgcag ggtgcggcgg aggagcttgc
      181 cgctgggacc gtgagggagc tcgtcgacga gctcgatctc ggccgggcac ttgtagcggg
      241 ccaggtggcc gtgggtgtgc tcccgcaggt cctcgaggcc gatgccggcc ccctcctcca
      301 acaccacgaa ggccttgacc gcctcgccgg tgcgggcgtt ggggacgccg accacggcgg
      361 cctcggccac atcagggtgg ctgaccagca cgtcctcgac ctcggccggg aagacgttga
      421 agccggacac gatgatgagg tccttggccc ggtcggacag gaagaggaag ccggactcgt
      481 cggcaaagcc gatgtcaccg gtgtgcagcc agccgtcgcc ggtgagcacg gccgtcgtcg
      541 cctccgggtc gttccagtag ccgacgaaga cgttgtcgcc ccggaccagg atctccccgg
      601 cgtcgccgat gaacgtgtcc tcgccgtcgg cgtcgatgat gcggacctcg acgcccggca
      661 gcgggcgccc gaccgaaccc accggggcgt cctggccgac gcccgtggcc accacgggcg
      721 aagtctcggt caggccgtac ccctcggcca gggccaggcc caggcgctcc tggacggact
      781 tgcggatctc ggggcccagg gcggcggcgc cggaggccgc ccgctcgacg gtcgccacgg
      841 cgtcggggct cgcgctctcg gctgcggcga ggccgtgcca catggcgggc gggcccgtca
      901 ggtcggtgac gccgtgggcg acgatggccc ggagcgcctc ctcggcgtcg aagcggtcga
      961 ggaggacgat gcgggacccg gccaccaagc cgccgcccag caccacgttc aggccgaaga
     1021 tgtggaacaa cgggatgacg ccgagcgcga cgtcatcggg gccccggcgt cggccttcgg
     1081 acgcctggac ctgctccaga ttggccacga ggttgccgtg ggtcagcatc gccgcccgcg
     1141 gggcgccggc cgtgccgctg gtgaacatga gcacggccag gtcggccgcc tcgcgttcga
     1201 cggtctcggc cggggcgtgg gccaccaggt cggccaggtc gtcgcccacg atgacatgct
     1261 cgaggaccgg cagcgcggcc cggtccaggt ccgccacgac cgtcgagccc tcgggcccgc
     1321 agaccacggc ccgggcgccg acggcgacca gctcccgggc cagctcaggc cccgggctcc
     1381 cgggattgag cggtacggcc acgaagccgc cccgggccgc ggccaaccag gtcagcacga
     1441 actcgacggt gttgcccgcc atgagcccca cccggtcgcc gacgtcgagg cccagctcgg
     1501 tcagtccccc ctgcaggccg gcgacctctc ggcgcagctc gccgtagctc acggtccggt
     1561 cgcccacgac cagggccacg gcgtcatcgg gatggccgtc gatgatgccc gccacgttca
     1621 ccgggtcagt ctgccccggc cggctccctc aaggcgaccc gtcccccctc cgatggggag
     1681 acgtggctct gaccgcatct cctccgcgcg ccgcgccccc ggtgacggcc atccggaccg
     1741 acggcctgac caaggccttc ggggagcacc gggccgtgga cggcctcacc ctcgacgtgg
     1801 cccccgggga ggtactggcc ctgttggggc ccaacggggc gggcaagacc acgaccgtcc
     1861 gcctgttgaa cggcgtgctg gccccggacg ccggcgccgc gcaggtcctg ggcatggacc
     1921 cggccgtcga cggagacgag gtccggcgtc ggaccggggt cctcaccgag aacgccggcc
     1981 tggatgaccg gctgaccgcc cgcgagaacc tcgtcttcac tgcccgcatc cgaggcatgg
     2041 ggatcgacga ggcccagtcc cggaccgcgg aggtgctcga tcagttcgcc atggccgacc
     2101 tggccgacgc ccgggtccac gggttctcca cc
//
LOCUS       DASTFV010000543         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2019326, whole genome shotgun sequence.
ACCESSION   DASTFV010000543 DASTFV010000000
VERSION     DASTFV010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2019326"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="VFP02_09935"
     CDS             complement(<1..1333)
                     /locus_tag="VFP02_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HET9543393.1"
                     /translation="MVADVTFIDGFVHQLPDTDPQETQEWLDSLDAVVSAQGEARARF
                     LLSKVLERSRQLGVGTPASVSTPYINTIPAEQEPWFPGDEHIERRIRAFIRWNAAVMV
                     IRANKKADGIGGHLSTFASSAAQYEVGFNHFFRGKADGLPGDAVYFQGHAAPGIYARA
                     YLEGRLTEDQLDHFRMEVGGEGLSSYPHPRLMPDFWEYPTVSMGLGPLNSIYHARFLR
                     YLHNRQVDDTSGSRVWCFVGDGEVDEPETLGSISLAAREGLDNLTWVVNCNLQRLDGP
                     VRGNGKIIQELEAIFRGAGWNVIKVVWGGRWDELLARDVDGVLVNKMNETVDGQYQRY
                     AVEDGAYAREHFFGPDPRLRELAADLSDDDIRNLPRGGHDYRKLYAAYKTATEQTGAP
                     TAILAKTVKGWTLGPGFEGRNATHQIKKMTVAELKTLRDRLHLHDEITDDAL"
     gene            1341..>2235
                     /locus_tag="VFP02_09940"
     CDS             1341..>2235
                     /locus_tag="VFP02_09940"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HET9543394.1"
                     /translation="MTTIVYTDGACLGNPGPGGWAWVVPGGAFASGADSHTTNQRMEI
                     EAATEALYAIDGPVNVFSDSTYVVHCFTKKWYVGWKAKGWKNSKKEDVANQDLWRPLI
                     DHVLERGNVTWTWVKGHSGDEWNDVADRLAVEAATTQVGRSGDRPPDFLGPADAVGKA
                     SAAPAASAASPTPAPASPSNRPDGHAVAVLGHRPPELGGYEPNFLQAAVQRRLGDVLA
                     AQAELDDDLVLLTGLQLGAEQLAAEAALEHGIPHVVVQPFPDPDSKWPSAARASYRRL
                     LAGAREVVLLQKKPVRSPGDVR"
BASE COUNT          331 a          738 c          828 g          338 t
ORIGIN      
        1 cgagggcgtc atcggtgatc tcgtcgtgga ggtggaggcg gtcccgcagc gtcttcagct
       61 cggcgacggt catcttcttg atctggtggg tggcgttgcg gccctcgaag ccggggccca
      121 gcgtccagcc cttcacggtc ttggccagga tcgccgtcgg ggcgcccgtc tgctccgtgg
      181 ccgtcttgta ggcggcgtag agcttgcggt agtcgtgccc gccccgaggc aggttgcgga
      241 tgtcgtcgtc ggacaggtcc gccgccagct cccgcaggcg agggtcgggc ccgaagaagt
      301 gctcccgggc gtaggccccg tcctccacgg cgtagcgctg atactggccg tcaacggtct
      361 cattcatctt gttgaccagg accccgtcga catcgcgggc cagcagctcg tcccaacggc
      421 caccccagac gaccttgatc acgttccagc ccgcgccccg gaagatggcc tccagctcct
      481 ggatgatctt cccgttgccc cggacggggc cgtcgaggcg ctgcaggttg cagttgacga
      541 cccacgtgag gttgtcgagg ccctcgcgag cggccaggga gatggagccc agcgtctcgg
      601 gctcgtcgac ctcgccgtcg cccacgaagc accagacccg ggagccgctg gtgtcgtcga
      661 cctgacggtt gtggaggtag cgcaggaacc gggcgtggta gatcgagttg agcggcccga
      721 ggcccatcga gaccgtgggg tactcccaga agtccggcat gagccgggga tgggggtagc
      781 tggagagccc ctcgcccccg acctccatcc ggaagtggtc gagctggtcc tcggtcaggc
      841 ggccctcgag ataggcccgg gcgtagatgc cgggggcggc atggccctgg aagtagacgg
      901 cgtcaccggg caggccgtcg gccttgcccc ggaagaagtg gttgaacccg acctcgtact
      961 gggcggcgga ggaggcgaag gtggagaggt ggccgccgat gccgtcggcc ttcttgttgg
     1021 cccggatgac catgacggcg gcgttccagc ggatgaaggc gcggatgcgg cgctcgatgt
     1081 gctcgtcgcc ggggaaccag ggctcctgtt cggccgggat cgtgttgatg tagggggtgg
     1141 acaccgacgc cggcgtcccg acgccgagct gccgggagcg ctcgaggacc ttggagagca
     1201 ggaagcgggc gcgggcctca ccctgggcgc tcaccacggc atccagggag tccagccact
     1261 cctgggtctc ctggggatcg gtgtcgggca actggtgcac gaagccgtcg atgaaggtca
     1321 catctgcaac catgccccgg atgacgacga tcgtctacac ggatggggcc tgtttgggga
     1381 atccgggccc cgggggctgg gcgtgggtcg tgccgggcgg ggcattcgcc agcggggccg
     1441 attcccacac caccaaccaa cgcatggaga tcgaggccgc caccgaagcc ctctacgcca
     1501 tcgacggccc cgtcaacgtc ttcagcgact ccacctacgt cgtccactgc ttcaccaaga
     1561 agtggtacgt gggctggaag gccaaggggt ggaagaactc gaagaaggag gacgtggcca
     1621 accaggacct ctggcgtccg ctgatcgacc acgtgctcga gcggggcaac gtgacctgga
     1681 cctgggtcaa gggccacagc ggcgacgaat ggaacgacgt ggccgaccgg ttggcagtgg
     1741 aggcggcgac gacccaggtg gggcggtcgg gggaccgtcc ccccgacttc ctggggccgg
     1801 ccgacgcggt cgggaaggcg tcggcggcgc cggctgcttc tgcggcctcg ccgacgccgg
     1861 ccccggcgag cccgtcgaac cggccggacg gccacgcggt ggcggtgctc gggcaccgac
     1921 ccccggagct ggggggctat gagcccaact tcctgcaggc ggcggtgcaa cgccgcctgg
     1981 gtgacgtgct cgccgcccag gccgagctcg acgatgacct ggtcctgctc accggtttgc
     2041 agctcggggc cgagcagctg gcggccgagg cggccctcga gcacggcatc ccccatgtcg
     2101 tggtgcagcc cttcccggac cccgacagca agtggccgtc cgccgcccgg gcgtcgtatc
     2161 ggcgactcct ggccggggcg cgggaggtgg tgctcctgca gaagaagccc gtgcgctcgc
     2221 cgggcgacgt ccgcc
//
LOCUS       DASTFV010000544         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2027034, whole genome shotgun sequence.
ACCESSION   DASTFV010000544 DASTFV010000000
VERSION     DASTFV010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2027034"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VFP02_09945"
     CDS             complement(<1..557)
                     /locus_tag="VFP02_09945"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9543395.1"
                     /translation="MSLDPWEAVLIVLAGVGAGGINAVIGSGSLITFPTLLAVGYSPV
                     VANVSNNIGMVPGSVFGSYGFRRELAGQKQRARILASASALGGLTGAILLLVLPSDVF
                     DTIVPILVLLACGLMLAQPRLAAWVAGRRAEGARDVGAAPLALGYLAGVYGGYFGAAQ
                     GVILLAILAVFVPDDLTRSNALKNVL"
     gene            638..1507
                     /locus_tag="VFP02_09950"
     CDS             638..1507
                     /locus_tag="VFP02_09950"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET9543396.1"
                     /translation="MGNSFLDGTVVHAVGDGRYEAAIDAVWNLRPLPQGGMVSALALR
                     AMATELDDPTQRLRTAHTSFAAQVADGSVDIDVELLRRGRSMSHLRAEVRNPGAARGH
                     LTTGIFGAARDGFTFTDLEPPADIPHPDDCPSFRDPLPPGVVDAFEPMPFWERVIEGR
                     NVKGHPPWEDYEPERAERVTWYAFDEPPLLDDGTLDPLALVVVADVMPGAVGERLGPR
                     AREWFAPSVDLTVHILDDCRSATLLGHNRARHAGDGYASVDMALWDLGLDQRDEPRLV
                     AYATQLFLFSFMD"
     gene            1536..1910
                     /locus_tag="VFP02_09955"
     CDS             1536..1910
                     /locus_tag="VFP02_09955"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET9543397.1"
                     /translation="MSNMARLEAIALVLPEATRVDIEAWDGEPTFRVRNKNFVFTDPE
                     VTAVSVKLPVEEAEAVVATDPLARPTGYGMGRAGWVSVDIGEGADEDRWQQVTEWVRT
                     SYTLVAPKTLARIVLEEDGLVD"
     gene            1928..>2207
                     /locus_tag="VFP02_09960"
     CDS             1928..>2207
                     /locus_tag="VFP02_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543398.1"
                     /translation="MHTYDVTVTWTGDEGEGTATYRSYRRDHVVSADGPPDLVGSSDP
                     ALRGDGSRWNPEQLLVASLSQCHMLWYLHLCALDDVVVTGYVDAAHGEM"
BASE COUNT          338 a          816 c          761 g          292 t
ORIGIN      
        1 agcacgttct tgagggcgtt ggaccgggtg aggtcgtcgg gcacgaacac ggcgaggatg
       61 gccagcagga tcacgccctg ggcggccccg aagtagccgc cgtagacgcc ggccaggtaa
      121 ccgagggcca gcggggcagc cccgacgtct cgggccccct ctgcccggcg gccggccacc
      181 caggccgcca gccggggttg ggccagcatg aggccacagg ccaggaggac gaggatcggc
      241 acgatggtgt cgaagacgtc ggagggcagg acaaggagga ggatggcacc ggtcaagcca
      301 cccagggcag aggccgaggc caggatccgg gcccgctgct tctggccggc cagctcccgt
      361 cggaacccgt aggacccgaa gaccgagccg ggcaccatgc cgatgttgtt ggacacgttg
      421 gccaccaccg gcgagtagcc gacggccagg agggtcggga aggtgatgag gctgcccgac
      481 ccgatgacgg cgttgatccc acccgcgccg acgccggcca gcacgatcag caccgcctcc
      541 cacgggtcga gactcaccgg tgaactctcg caggtacctt cccgcggcag gccgcaatcg
      601 ggttgacgcc gggcggcggt gcggcgagcg tgcccccgtg ggcaactcct tcctggacgg
      661 caccgtcgtc cacgccgtcg gcgacggccg ctacgaggca gccatcgacg cggtctggaa
      721 cctgcgcccc ctcccccagg ggggcatggt cagcgccctc gccctgcggg ccatggccac
      781 cgagctggac gacccgaccc agcgcctgcg cacggcccac acctcgttcg cggcccaggt
      841 ggccgacggg tccgtggaca tcgacgtgga gctgctgcgc cggggacgct ccatgtccca
      901 cttgcgggcc gaggtccgca acccgggggc ggcccggggc cacctgacca ccgggatctt
      961 cggtgccgcc cgggacgggt tcaccttcac cgacctcgag ccccccgccg acatccccca
     1021 ccccgacgac tgcccctcgt tccgggaccc actccccccc ggcgtggtcg acgccttcga
     1081 gccgatgccg ttctgggaac gggtcatcga gggtcgcaac gtgaagggac acccgccgtg
     1141 ggaggactac gagcccgaac gggccgagcg ggtcacctgg tacgccttcg atgagcctcc
     1201 actgctcgac gacgggaccc tggaccccct cgccctggtc gtcgtggccg acgtcatgcc
     1261 cggcgccgtc ggcgagcggc tggggccgcg cgcccgtgag tggttcgccc ccagcgtcga
     1321 cctgaccgtc cacatcctgg acgactgccg ctcggccacc ctgctgggcc acaaccgagc
     1381 ccgccacgcc ggtgacggct atgcctcggt cgacatggca ctctgggacc tcggcctcga
     1441 ccagcgcgac gagccccgcc tggtcgccta cgccacccag ctcttcctct tcagcttcat
     1501 ggactgacgt gccccgcggt ggaacactcg gggccatgag caacatggcc cggctggagg
     1561 ccatcgccct cgtcctgccc gaggcgacaa gggtggacat cgaggcgtgg gacggtgagc
     1621 ccacgttccg ggtccgcaac aagaacttcg tcttcaccga tcccgaggtc accgccgtct
     1681 cggtgaagct gcccgtcgag gaggccgagg ccgtcgtggc caccgatccc ctggcccggc
     1741 ccacgggcta cgggatggga cgcgccggct gggtctcggt cgacatcggc gagggcgccg
     1801 acgaggaccg ctggcagcag gtcaccgagt gggtgcggac ctcctacacc ctggtggccc
     1861 ccaagaccct ggcccggatc gtcctcgagg aggacggcct cgtcgactga cgatggagac
     1921 tgcggaggtg cacacctacg acgtgaccgt gacctggacc ggcgacgagg gcgagggcac
     1981 ggcgacctac cgcagctatc gacgggacca cgtggtctcc gccgacggac cgcccgacct
     2041 ggtcggctcc tcggatcccg ccctccgggg tgacggcagc cgttggaacc ccgagcaact
     2101 gctcgtggcc tcgctctcgc agtgccacat gctctggtat ctccacctgt gcgccctcga
     2161 cgacgtggtc gtcaccggct acgtggatgc ggcccacggt gagatgg
//
LOCUS       DASTFV010000545         3345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2030573, whole genome shotgun sequence.
ACCESSION   DASTFV010000545 DASTFV010000000
VERSION     DASTFV010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2030573"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VFP02_09965"
     CDS             complement(<1..1188)
                     /locus_tag="VFP02_09965"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543399.1"
                     /translation="MPALGRAFDYLVPDGMDGRVGVGSLVRITLHGRRVGGWVIGLDS
                     TPPDGTVLQPLAKVTGWGPPPDLVDLADWAAHRWAGRSVAFLRTASPPRVVTGVPDPW
                     TPSHLVPTTDDQLAESAFSANGVWQPTVLRLAPGADRFPLVLSACRRGDALVLLPSVG
                     DAEALGVRLRRAGVPVAVMPDEWAQARGGATVVGARSAAWAPVVDLAAVLVLDEHDEA
                     HKQEQTPAWHARDVVVERARRAGVPCVLASPCPSLEALEAGRLVTQARSVEREGWPIV
                     DVIDRRQDEPGRTGLYSPRLVELMRGGGRVLCVLNRKGRARLLACAACGEMAGCERCG
                     AALGQAEPGTLSCLRCDHSRPTVCAAGGGTRMKLLRVGVTRAAEELAALARTEVVEVT
                     GESA"
     gene            complement(1264..2457)
                     /gene="metK"
                     /locus_tag="VFP02_09970"
     CDS             complement(1264..2457)
                     /gene="metK"
                     /locus_tag="VFP02_09970"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET9543400.1"
                     /translation="MSKFTFTSESVTEGHPDKMADQISDSILDAMLAEDPASRVACET
                     LVTTGIAIVAGEITTNAYVDIPGTVRQTINDIGYDRESFGYDGNTCGVMVCIDEQSAD
                     IAQGVDDSEEVRSGTSGEDSLNKQGAGDQGMMFGYACDETDVLMPLPIHLAHRMAERL
                     TEVRRAGTVPYLRPDGKTQVTFDYEDGKPVRLTTVLISTQHNDGIDRDEVIRPDLIEH
                     VIRPVIPEQYTDDEYEVFVNPTGRFVTGGPVGDAGLTGRKIIVDTYGGMARHGGGAFS
                     GKDPSKVDRSAAYAARWVAKNLVAAGAASRCEVQVAYAIGVAHPISIMVETFGTENVD
                     KVKLEETVREVFDLRPAAIIRDLELRQPIFRQTAAYGHFGRETEGFTWERRDRVDAVK
                     SALGL"
     gene            complement(2492..3175)
                     /locus_tag="VFP02_09975"
     CDS             complement(2492..3175)
                     /locus_tag="VFP02_09975"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HET9543401.1"
                     /translation="MAALEAALGPDDLAGLDVLITAGGTREPIDPVRFVGNRSSGKQG
                     HALADEAVSRGAKVTLVTTVERAVAHGVEVVMVATAAEMDAAVRERSDRADVVVMAAA
                     VADFRPDTAAVAKLKKEGGPPTVTLVPTDDILGGLLQRRWPGQTLVGFAAETDDVDAN
                     ATAKLARKPVDLLVVNDVSQPGAGFESDTNEVRIMSPAGSTVTVDQRDKRAVARAVLD
                     AVVAHRATP"
     gene            3257..>3345
                     /locus_tag="VFP02_09980"
     CDS             3257..>3345
                     /locus_tag="VFP02_09980"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HET9543402.1"
                     /translation="MQVLVTAGGTREPIDAVRVIANRSSGKQG"
BASE COUNT          451 a         1191 c         1238 g          465 t
ORIGIN      
        1 cgccgactcc cccgtcacct ccaccacctc ggtccgggcc agcgccgcca actcctcggc
       61 cgcccgggtc acccccaccc gcagcagctt catccgggtg ccaccgcccg ccgcgcacac
      121 cgtcggtcgg ctgtggtcac agcgcagaca ggacagtgtc cccggctcgg cttggccgag
      181 cgccgcaccg caccgctcgc agccggccat ctccccgcac gcggcacagg ccagcaggcg
      241 ggcccggccc ttgcggttga gcacgcacag caccctcccg cccccccgca tcagctcgac
      301 caggcggggt gagtagagcc cggtccgccc gggctcgtcc tgacggcggt cgatgacgtc
      361 gacgatcggc cacccctccc gctcgacgga gcgggcctgg gtcaccagcc gtcccgcctc
      421 gagggcctcc agcgacgggc acggcgaggc cagcacgcag ggcacgccgg cccgccgagc
      481 ccgctcgacg accacgtccc gggcgtgcca ggcgggggtc tgctcctgct tgtgggcctc
      541 gtcgtgctcg tccagcacca gcacggcggc caggtcgacg acgggggccc aggcggccga
      601 tcgggccccg acgaccgtcg ccccaccccg ggcctgggcc cattcgtcgg gcatcacggc
      661 cacgggcaca cccgcccgtc gcagccgcac ccccagcgcc tccgcgtcac ccaccgatgg
      721 caggagcacc agcgcgtccc cgcgccggca ggccgacagg accagcggga accggtcggc
      781 gcccggggcc agccgcaaca ccgtcggctg ccaaaccccg ttggcggaga acgcggactc
      841 ggccaactgg tcgtcggtgg tggggacgag gtggctgggc gtccaggggt cggggacgcc
      901 ggtcaccacc cgaggcggcg acgccgtgcg caggaaggcg acggagcggc cggcccagcg
      961 gtgggcggcc cagtcggcga gatcgaccag gtccgggggt gggccccacc ccgtgacctt
     1021 ggccagcggc tgcaggaccg tgccgtccgg gggggtcgag tccagaccga tgacccaacc
     1081 gcccacccgg cggccgtgca gcgtgatgcg caccagggaa ccgacaccga cccgaccgtc
     1141 catcccgtca ggaacgaggt agtcgaaggc gcgccccagt gcgggcacgt cgggcaggac
     1201 ccggacgatc gacccgggtc gggcggtggg ttctcctggt ggcgagtgtg acgtggtggc
     1261 gttctacagg ccgagcgcgg acttcaccgc gtcgacccgg tcccgccgct cccacgtgaa
     1321 gccctccgtc tcgcggccga agtggccgta ggccgcggtc tggcggaaga tcggctggcg
     1381 cagctcgagg tcccggatga tggcggccgg gcggaggtcg aagacctcac ggaccgtctc
     1441 ctccagcttg accttgtcca cgttctcggt cccgaaggtc tcgaccatga tcgagatggg
     1501 gtgcgccaca ccgatggcgt aggccacctg gacctcgcac cggctggcgg cgccggcggc
     1561 gaccaggttc ttggccaccc agcgggcggc gtaggcggcc gagcggtcga ccttgctggg
     1621 gtccttgccc gagaaggcgc caccgccgtg gcgggccatg ccgccgtagg tgtcgacgat
     1681 gatcttgcgg ccggtgaggc cggcgtcgcc cacgggaccg ccggtcacga agcgaccggt
     1741 cgggttgacg aagacctcgt actcgtcatc ggtgtactgc tcggggatca ccggacggat
     1801 gacgtgctcg atcaggtcgg ggcggatgac ctcgtcgcgg tcgatgccgt cgttgtgctg
     1861 cgtggagatc aggacggtgg tgaggcggac aggcttgccg tcctcgtagt cgaaggtgac
     1921 ctgggtcttg ccgtccgggc gcaggtacgg gaccgtgccc gcccgacgga cctcggtgag
     1981 gcgctcggcc atgcggtggg ccaggtggat cggcagcggc atgagcacgt cggtctcgtc
     2041 acaggcgtag ccgaacatca tcccctggtc gccggcgccc tgcttgttga gcgagtcctc
     2101 gccggaggta ccggagcgga cctcttcgct gtcgtccacg ccctgggcga tgtcggcgga
     2161 ctgctcgtcg atgcagacca tgacgccgca ggtgttgccg tcgtagccga acgactcccg
     2221 gtcgtagccg atgtcgttga tggtctggcg cacggtgccc gggatgtcga cgtaggcgtt
     2281 ggtggtgatc tcgccggcga cgatggcgat gcccgtggtg accagcgtct cgcaggcgac
     2341 ccggctggcg ggatcctcgg ccagcatggc gtcgaggatc gagtccgaga tctggtcggc
     2401 catcttgtcg ggatggccct cggtgacgct ctccgaggtg aaggtgaact tgctcactgg
     2461 tgctccatgg tcgggggtgt gacgatcggg ttcagggcgt ggcgcggtgg gcgaccacgg
     2521 catcgaggac ggcacgggcg acggcccgct tgtcacgctg gtcgacggtc accgtcgatc
     2581 cggcgggcga catgatacgc acctcattcg tgtccgactc gaacccggca ccgggctggg
     2641 agacgtcgtt caccaccagg aggtcgacgg gcttgcgggc cagcttggcc gtggcgttgg
     2701 cgtcgacgtc gtcggtctcg gcggcgaagc cgaccagggt ctgaccgggc cagcggcgct
     2761 ggagcaggcc cccgaggatg tcgtcggtcg gcacgagggt gaccgtggga gggccaccct
     2821 ccttcttcag cttggcgacc gccgcagtgt cgggtcggaa gtcggccacg gcggcggcca
     2881 tgacgaccac atcggcccgg tcgctgcgct cgcggacggc ggcgtccatc tcggccgcgg
     2941 tggccaccat caccacctcg acaccgtggg cgaccgcgcg ctcgacggtg gtgaccaggg
     3001 tgaccttggc cccccgggac acggcctcgt ccgccagggc gtggccctgc ttgccggagg
     3061 agcggttgcc gacgaagcgc acggggtcga tgggctcgcg ggtgccaccc gcggtgatca
     3121 gcacgtcgag gccggccagg tcgtcgggtc cgagggcggc ctcgagggcg gccacgatgt
     3181 gttccgggtc ggccaggcga ccggcaccga cgtcgccgcc gtcgaggccg tcctcgggcc
     3241 ccgggacctc gcgggcgtgc aggtgctcgt caccgccggc gggacgcggg agccgatcga
     3301 cgccgtgcgc gtcatcgcca accgctcgtc gggcaagcag ggcta
//
LOCUS       DASTFV010000546         5327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2039418, whole genome shotgun sequence.
ACCESSION   DASTFV010000546 DASTFV010000000
VERSION     DASTFV010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2039418"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..509
                     /locus_tag="VFP02_09985"
     CDS             228..509
                     /locus_tag="VFP02_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HET9543403.1"
                     /translation="MRPADETETDWMAYGHCADRPPSLFFPSDGVGVDVARRICADCP
                     VKELCLEYALTNRIDHGVWGGCSERERRRILKRRRQEAEPTATETPTGA"
     gene            complement(516..1211)
                     /locus_tag="VFP02_09990"
     CDS             complement(516..1211)
                     /locus_tag="VFP02_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543404.1"
                     /translation="MTEQDATSDGPEDEAMRRLLADLPEVTPPDGFFDDLIRRRRRRA
                     RIIAGAGLAAAGVAGAIVVAHVTGITGHVDPETAMLADRHATVLTVDVRSIGEQLAGD
                     EVPAPYQAPEELGGMQRGMAVLHPDDIVQVVYAADGHYLSLFEQAGDMDDDAMEEDLT
                     PVDGGDAWRTDDGAVIVRRSDVVYVLVGDVADLSPETLAAIVAELPDARRMGLARRIG
                     DAMDDLVDAFGLN"
     gene            complement(1208..1705)
                     /locus_tag="VFP02_09995"
     CDS             complement(1208..1705)
                     /locus_tag="VFP02_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9543405.1"
                     /translation="MPSWEEVALHYGRFLYTVAYRLTMNHDDAQDLVQDVLLRVQRGL
                     ATYQPGSMEAWLSRITTNAFLDKVRRQKRRPTDPLPEEHPERVLPTSASAAEALDAAV
                     LPDHLQDALRALPEDYRTAVVLCDVLDFSYEEIAEALDIPVGTVRSRIHRGRARLRAA
                     VEAMS"
     gene            complement(1826..2002)
                     /gene="tatA"
                     /locus_tag="VFP02_10000"
     CDS             complement(1826..2002)
                     /gene="tatA"
                     /locus_tag="VFP02_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET9543406.1"
                     /translation="MNLGAPELIIVLLIILLIFGGSKLPQLARSLGKAQSEFKKGSEE
                     DAVTTDRSDPPPPV"
     gene            2063..2851
                     /locus_tag="VFP02_10005"
     CDS             2063..2851
                     /locus_tag="VFP02_10005"
                     /inference="COORDINATES: protein motif:HMM:NF019454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543407.1"
                     /translation="MLECIVNVSEGRDRDVLAALRDAGAHDLLDVHVDAHHHRCVLTT
                     VGEDAARRIARVAVARVDLRTHVGAHPRLGAVDVVPFVALDDAAPADACAARSRFAAW
                     IADELAVPAFTYGEGDGVPALPEVRRRAFVDLWPDTGPGRPHPTAGAVAVGCRPPLVA
                     YNLWLAEPDLDLARSVASAVRGDGIRALGLAVGDRVQVSMNLIDPHRVGPATAYDRVA
                     ERAAVAGAELVGLLPRRVLDAVAPERWGELDLAADRTVEARRAR"
     gene            complement(2856..3140)
                     /locus_tag="VFP02_10010"
     CDS             complement(2856..3140)
                     /locus_tag="VFP02_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HET9543408.1"
                     /translation="MRSIFDDNEDLTWQEYANCLGVDPDLFFPERGASTREAKEVCRG
                     CVVRDDCLEYALTNSEKFGIWGGMSERERRRIRRQRAIAREAATITVHTA"
     gene            complement(3323..>5327)
                     /locus_tag="VFP02_10015"
     CDS             complement(3323..>5327)
                     /locus_tag="VFP02_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543409.1"
                     /translation="YSRWDGTQVGFELGADEVMAEITDDLLYHGDLNAALRRMLQSGF
                     DTPDGEHLQGLREIMERLRQQRREMLENHDLGGVYDEIADELREVIDQERASLQEMAR
                     DAAESGNQRRQEVTDEVVQQRQMDLDLLPPDLAGQVKALQSYEFTSSEAREKFEELLD
                     RLREQLMQRFVDQVSGSMQSMSPEDMARMKDMLAELNTMLEQRAQGQEPDFDGFMERY
                     GDFFPENPQSLDELLELMAQRMAAMQAMLNSMTPEQRAQLQSLSDQLLEDMDLRWQVD
                     QLGQNLQAAFPDMNWNRSYDFSGQDPMGFGEAASVMEQLGDLDQLENLLRNATSPGAL
                     AEVDLDKVKEHLGEDSAESLEKLAELAKLLEEAGLIENKEGRFELTAKGMRRIGQNAL
                     AELFKRLSVDKMGGHQVERVGTGHERAYDTKPYEFGDPFNLHIERTLRNAVGRQGPGT
                     PVRLQPDDFEVERTEHLVRSSTVLMLDLSLSMPMRDNFLPAKKVAMALHSLISSRFPR
                     DYLGIVGFSSVARALTPDQLPEVSWDYEYGTNMEHGFQLSRRMLARQSGTKQIIMITD
                     GEPTAHNLPNGEVYFNYPPVRETVEATMREVAKCTKENIRINTFMLDPNGHLKEFVER
                     LTRFNQGRAFFTTPETLGDYVLVDFIEQKRAMVRGRTGGRRSA"
BASE COUNT          740 a         1811 c         1908 g          868 t
ORIGIN      
        1 gcgtcgatga cggcccgatc cccgaaatcg gagcggatct cccagtgcgg ggcgaccgga
       61 ccgaggtaca cgatcttcac cgcgccccgg ggcggctcgg tctcgaggga gggttcggcg
      121 acgttcgaca tcggtttcag gttaccgggg aacctcgggc atgcttttcg ggaaccaaag
      181 caggtcgccc gccgtagtag agggtgaccg gaggtatttc caggcccatg cgccccgccg
      241 atgagaccga aaccgactgg atggcttacg gccactgcgc cgatcgaccg ccctcgctgt
      301 tcttccccag cgacggcgtc ggcgtcgatg tggcccggcg gatctgcgct gactgcccgg
      361 tcaaggagct ctgcctggag tacgccttga ccaaccgcat cgaccacggc gtgtggggtg
      421 gctgctccga gcgggagcgc cgtcgcatcc tgaagcggcg ccgccaagag gccgaaccca
      481 cggcgaccga gaccccgacc ggggcctagc tcagctcagt tgaggccgaa ggcgtcgacg
      541 aggtcgtcca tggcatcgcc gatgcggcgg gccaggccca tccggcgggc gtcaggcagc
      601 tcggccacga tggcggccaa ggtctccggc gagagatcgg cgacatcgcc gaccagcacg
      661 tagaccacgt ccgaccggcg cacgatcacg gccccgtcgt cggttcgcca ggcgtcacca
      721 ccgtcgaccg gcgtgaggtc ttcctccatg gcgtcatcgt ccatgtcccc ggcctgctcg
      781 aagagggaga ggtagtggcc gtcggcggcg tagaccacct ggacgatgtc gtcggggtgc
      841 aggaccgcca tcccccgctg catcccgccc agctcctcag gcgcctggta cggcgccggg
      901 acctcgtcgc ccgccagctg ctcgccgatc gagcgcacgt cgacggtgag caccgtggcg
      961 tgacggtcgg ccagcatggc cgtctcgggg tcgacgtggc ccgtgatgcc ggtgacatgg
     1021 gccacgacga tcgccccggc cacgcccgcc gcggccaggc cggcaccggc gatgatccgg
     1081 gcccgacgcc ggcgccgacg gatcaggtcg tcgaagaagc cgtcgggggg cgtcacctcc
     1141 ggcaggtcgg ccagcagacg gcgcatggcc tcgtcctcgg gcccgtccga ggtcgcgtcc
     1201 tgctcggtca cgacatcgcc tcgaccgcgg cccgtaggcg ggctcggccc cggtggatgc
     1261 gggagcggac ggtccccacg gggatgtcca gcgcctcggc gatctcctcg tagctgaagt
     1321 cgagcacgtc acagagcacg acggcggtgc ggtagtcctc gggaagcgcc cggagggcgt
     1381 cctggaggtg atcgggcagg acggcggcgt cgagggcctc ggcggccgag gccgacgtcg
     1441 gcaggacgcg ctcggggtgc tcctcgggca gcggatcggt gggacggcgc ttctggcggc
     1501 ggaccttgtc caggaaggcg ttggtggtga tccgcgacag ccaggcctcc atggagcccg
     1561 gctggtaggt cgccagccct cgttgcactc gtaacagcac gtcctgcacc agatcctgcg
     1621 cgtcgtcgtg gttcatcgtg agccggtagg ccaccgtgta gaggaaccgc ccgtagtgaa
     1681 gggcgacctc ctcccagctc ggcacctcgt cggcgcgagg tggtgggggc gttgggctcg
     1741 acatgggaac gacgccggga cgcgcccggt tcccgatcga ggctagcgat cagtcagcgc
     1801 caggctgagg actccgagga gtcggtcaga cgggaggggg cggatcgctg cgatccgtcg
     1861 tcactgcgtc ttcttcggag cccttcttga actcggactg ggccttgccc agggagcgag
     1921 cgagctgcgg cagcttggag ccgccgaaga tgagcaggat gatcaggagc acgatgatga
     1981 gttcgggtgc accgaggttc acatctgcga gcgtaccgtc ggccggctcc ggcctgcggt
     2041 catgagtcag gaggcgacga gcgtgctcga gtgcatcgtc aacgtgagcg agggtcgcga
     2101 ccgcgacgtc ctcgccgccc tgcgcgatgc cggcgcccat gacctcctcg acgtgcacgt
     2161 cgacgcccac caccaccgct gcgtcctcac cacggtgggc gaggacgccg cccggcggat
     2221 cgcccgggtc gccgtggccc gggtcgacct ccgtacccac gtcggggccc acccccggct
     2281 cggggccgtc gacgtcgtgc ccttcgtggc cctcgacgac gccgcgccgg cggacgcctg
     2341 tgccgcccgg tcacggttcg cggcgtggat cgccgacgag ttggccgtgc ccgcgttcac
     2401 ctacggggag ggtgacgggg tgccggcgct gcccgaggtc cggcgccggg ccttcgtgga
     2461 cctctggccc gacaccggac cgggccggcc ccatccgacg gccggggcgg tggcggtggg
     2521 gtgtcgcccg ccgctggtgg cctacaacct ctggctggcg gaaccggacc tggacctcgc
     2581 ccgctcggtg gcctcggcgg tgcggggcga cggcatccgg gccctggggc tcgccgtcgg
     2641 ggaccgggtc caggtgtcga tgaacctgat cgacccgcac cgggtggggc cggcgacggc
     2701 ctacgaccgg gtggcggaac gggccgcggt ggccggggcg gagctggtgg ggctgctccc
     2761 ccgccgggtc ctcgacgccg tggcgcccga acgttggggg gagctggacc tggccgcgga
     2821 ccggacggtg gaggcccggc gggcccgctg acggcctacg cggtgtggac ggtgatggtg
     2881 gcggcttccc gggcgatggc ccgctgacga cggatgcggc ggcgctcgcg ctcgctcatg
     2941 ccgccccaga tgccgaactt ctcactattg gtgagggcgt actccaggca gtcgtcgcgc
     3001 acgacgcagc cccgacagac ctccttggcc tcccgggtgg acgcaccccg ctcggggaag
     3061 aagaggtccg gatcgacgcc gaggcagttg gcgtactcct gccacgtcag gtcctcgttg
     3121 tcatcgaaga tcgatctcat tggtgtgcct ccttgttggc gcaccgcctg ttgcttcccc
     3181 aagcatcccg gtgttcggga caccggtgta attacacttc tgtcatcatt ccaccggttg
     3241 ccccctcggc gcaagggcgc gtcgaggaaa tccctgctgc accggtaatc gtgggtcggg
     3301 cctcgcgggc ccgagcgggg gctcaggcgg agcgacgacc gcccgtacgg ccccggacca
     3361 tggcccgctt ctgctcgatg aagtcgacga ggacgtagtc accgagcgtc tcgggggtgg
     3421 tgaagaaggc ccggccctgg ttgaaccggg tcaggcgctc cacgaactcc ttcaggtgcc
     3481 cgttcgggtc gagcatgaag gtgttgatgc ggatgttctc cttcgtgcac ttggccacct
     3541 cgcgcatggt ggcctcgacg gtctcccgca ccggcgggta gttgaagtac acctcgccgt
     3601 tgggcaggtt gtgggcggtc ggctcgccgt cggtgatcat gatgatctgc ttggtgccgg
     3661 actggcgggc cagcatccgg cgactcagct ggaacccgtg ctccatgttg gtgccgtact
     3721 cgtagtccca ggagacctcg gggagctggt cgggcgtcag ggcccgggcc acgctgctga
     3781 agccgacgat ccccaggtag tcccgcggga agcggctgga gatgagcgag tgcagcgcca
     3841 tcgccacctt cttggcgggc aggaagttgt cccgcatcgg catcgacaac gagaggtcga
     3901 gcatcaacac ggtgctcgac cggaccaggt gctcggtgcg ctcgacctcg aagtcatcgg
     3961 gttgcaaccg cacgggggtg cccgggccct gacgaccgac ggcgttgcgc agggtgcgct
     4021 cgatgtgcag gttgaacggg tcaccgaact cgtagggctt ggtgtcgtag gcccgctcgt
     4081 gaccggtacc cacgcgttcg acctggtggc cccccatctt gtccaccgag aggcgcttga
     4141 acagctcggc caacgcgttc tggccgatgc ggcgcatgcc cttggcggtg agctcgaagc
     4201 gaccctcctt gttctcgatc aggccggcct cctccagcag cttggccagc tcggccagct
     4261 tctcgaggct ctcggccgag tcctcgccca ggtgctcctt gaccttgtcg aggtcgacct
     4321 cggccagcgc gcccggtgag gtggcgttgc gcagcaggtt ctcgagctgg tcgaggtcgc
     4381 ccagctgctc catcaccgac gccgcctcgc cgaagcccat cgggtcctgg ccggagaagt
     4441 cgtagctccg gttccagttc atgtcgggga aggcggcctg caggttctgg ccgagctggt
     4501 ccacctgcca ccgcaggtcc atgtcctcga ggagctggtc ggacaggctc tggagctgcg
     4561 cccgttgctc gggcgtcatc gagttgagca tcgcctgcat cgcggccatg cgctgggcca
     4621 tgagctccag cagttcgtcg aggctctggg ggttctccgg gaagaagtcg ccgtagcgct
     4681 ccatgaaccc gtcgaagtcg ggctcctggc cctgggcccg ctgctcgagc atcgtgttga
     4741 gctcggccag catgtccttc atccgggcca tgtcctcggg cgacatcgac tgcatcgagc
     4801 ccgacacctg gtccacgaag cgctgcatga gctgctcgcg cagccggtcg agcagctcct
     4861 cgaacttctc acgggcctcc gaggaggtga actcgtagct ctgcagcgcc ttcacctggc
     4921 cggcgaggtc cggcggcagg aggtcgaggt ccatctggcg ctgctggacg acctcgtcgg
     4981 tcacctcctg gcgccgttgg ttccccgact cggcggcgtc ccgggccatc tcctgcaacg
     5041 aggcccgctc ctggtcgatg acctcccgga gctcgtcggc gatctcgtcg tagaccccgc
     5101 cgaggtcgtg gttctccagc atctcgcggc gctgctggcg caggcgttcc atgatctccc
     5161 gcaagccctg gaggtgctcg ccgtcggggg tgtcgaagcc cgactggagc atgcggcgca
     5221 gggcggcgtt gaggtcaccg tgatacagca ggtcgtcggt gatctccgcc atgacctcgt
     5281 cggcgccgag ctcgaagccg acctgggtgc cgtcccagcg cgagtaa
//
LOCUS       DASTFV010000547         5043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2039468, whole genome shotgun sequence.
ACCESSION   DASTFV010000547 DASTFV010000000
VERSION     DASTFV010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2039468"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..89)
                     /locus_tag="VFP02_10020"
     tRNA            complement(4..89)
                     /locus_tag="VFP02_10020"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53..55),aa:Leu,seq:taa)
     gene            complement(118..426)
                     /locus_tag="VFP02_10025"
     CDS             complement(118..426)
                     /locus_tag="VFP02_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543410.1"
                     /translation="MEATSVRFAGAVKSLSDVARRQGLQAPAFRSPPRLAGCERTIRW
                     RSDGGATVAVRLRGRLWVQVLADMVEGVVAANGLQGPEADQARTALWASVEGGGLQAA
                     "
     gene            509..1441
                     /locus_tag="VFP02_10030"
     CDS             509..1441
                     /locus_tag="VFP02_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET9543411.1"
                     /translation="MELSPLNPAQQEVVDLLGAPRSEWPEFDPDLRTDLRGMLDDALS
                     PLADGLGEETLWVGKYKLKEVHGCEVRFVAEQAGFVPSAATARGTVTHKAIELTLNLP
                     GDTPPLDLVDEAIARLQDSDAWLAEWLRTCDEAERAELRGEVNDLLVKFLDCWPPLRP
                     QWRPAPESRLNYDLFGGTIVLAGKVDLALGRAEGTRAGKVLIDLKTGRSSPLHREDLR
                     FYALIETLRLGVPPRRIATHYLDSGHLQIEDVSLDVLEAAVARTVDGIAAMVELRDPE
                     RPLQKKPGTACGWCALAENCAEGQEWLTDESRAY"
     gene            complement(1446..2735)
                     /locus_tag="VFP02_10035"
     CDS             complement(1446..2735)
                     /locus_tag="VFP02_10035"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9543412.1"
                     /translation="MSSAEPADVEEEIRPGLRSAVAAFRYRNFALFWTGAVVSNTGGW
                     VQNVTVPYVVFRLTDSAAWVGFASFSLLITASILGPVGGTLADRFDRRTVLLVMSTAQ
                     AAVAMALWAAWGAGVRSPGVIIAMAATSGVFAGLMIGSWQAFVSELVPREALLNAVTL
                     NSAQFNAARAFGPAIGGVILALVGPGWAFFVNGVSFLAVIAALLAMRLDRVVEKAVGR
                     MHIVADFRETIRYVRASAGIAACVIVVAALGFFESPVFQLVVVFAEDVFVVDEWVFGV
                     LSAALGIGAILATPFVAGWGSTFRRQVLVAGAILLCGTSMAVFALAPNAYVGFVGLLG
                     VGAGYLTIASSLNTTIQLQVDEQMRGKVLAFYVLSITLCAPLGSLLQGALAEVIGPRA
                     TVAAGGVCFVLVLVLLRARGSLAHLDDEVAQPLIDPV"
     gene            2870..3781
                     /locus_tag="VFP02_10040"
     CDS             2870..3781
                     /locus_tag="VFP02_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9543413.1"
                     /translation="MASEDTPVDTSPAVAEGLPAEVPGGTLVRLPMDRSASGREDPAR
                     QGDVDEWGRSEHMRELARKLYDPIYRSWFRAEWEGLEKIPEHGGALLVANHAGAVPSD
                     APVIMHGIESELERPVYGLADHLFKSLPVVGTLWSRVGGVVAHPDNAYRLLHDKRALV
                     LVFPEGTKGTGKHVSERYRLRRFGRGGFVEIAMKAGVPVIPIAVVGSEEAMPIIAKIN
                     PLAKALNLPYVPITPQMLALGPLGLVAPLPAKFRLRVLDPIHFDVPAGQERYSKSKVM
                     DAADDIRQRIQDQLFDMIRTRRSIWKG"
     gene            3783..4829
                     /locus_tag="VFP02_10045"
     CDS             3783..4829
                     /locus_tag="VFP02_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9543414.1"
                     /translation="MGRRVLVTGLSTFWGGLVAQQLEADPDVEVIVGLDTSPPEIELE
                     RTEFVRVDQNYSILSRIVAATKVDTIIHTFLVMNTGQVSSRAMKEINVIGTMNLFAAA
                     SAPGSTVERVVVKSSSLVYGADREDPSFFREDMARTHHPKTGIERSLLAVEDYVRDFA
                     VDNPHVTVSLLRFSNVLGPTISTPFSKLLHLPLVPSVFGFDPRFQFVHEDDVVRSILF
                     VLDEDLAGIYNVAGDGMLPWSEVATICGKRTVPLPPLATGAAALPLRALGYDMPPETL
                     DLLRYGRGVDGTKLVDAGFQYRFTSAGTVENFAQALRLRSTVGARDPEYTYQADVEQF
                     FKHSPSVVRDVPLS"
     gene            4826..>5043
                     /locus_tag="VFP02_10050"
     CDS             4826..>5043
                     /locus_tag="VFP02_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET9543415.1"
                     /translation="MSDAVLVDVVDRVATVTLNEPDKRNAISLPMVGGIAEAFDALEA
                     DEAVGAIVVTGAPPAFCAGADLSHLGSSR"
BASE COUNT          801 a         1832 c         1700 g          710 t
ORIGIN      
        1 cagtgcccgg ggtgggattc gaacccacac ggccccgagg ggccaggggg gtttaagccc
       61 cccgcggcta ccagttacgc cacccgggcc ggcccggcca ccgtaaccag gtgccggtca
      121 ggcggcttgg aggccgccgc cttctacgga cgcccagagc gcggtgcggg cctggtcagc
      181 ttcgggaccc tggaggccgt tggcggcgac gacgccctcc accatgtcgg ccaggacctg
      241 aacccagagg cggccccgga ggcggacggc gaccgtcgcc ccaccgtcgg atcgccatcg
      301 gatggtgcgc tcgcagccgg cgaggcgcgg cgggctacgg aacgcggggg cttggaggcc
      361 ctgccgacgg gccacgtcac tgaggctctt caccgctccc gcgaaccgca ccgacgtggc
      421 ttccatcggc cccattctcc ccagggggtg tgacggccat cggatgctcc ccgcgccagt
      481 cacggcatcg gcgtaccgtc ggttcgagat ggagctctcg cccctgaacc ccgcccagca
      541 ggaggtcgtg gatctcctcg gcgcgccccg ctccgagtgg ccggagttcg acccggacct
      601 gcgcacagac ctgcgcggga tgctggacga cgccctctcc ccgctggccg acggcctggg
      661 tgaggagacc ctctgggtcg gcaagtacaa gctgaaagag gtacacggct gtgaggtccg
      721 cttcgtagcc gagcaggcag ggttcgtgcc ctcggccgcg acggcgcgcg gcaccgtgac
      781 gcacaaggcc atcgagctca ccctgaacct cccgggggac acaccgcccc tcgacctggt
      841 cgacgaggcc atagcccgcc tccaggacag cgatgcctgg ctcgccgagt ggttgcggac
      901 ctgtgacgag gccgaacggg ccgagctgcg gggcgaggtc aacgacctgc tcgtcaagtt
      961 cctcgactgt tggccgcccc ttcgcccgca gtggcggccc gcgccggaga gtcgactcaa
     1021 ctacgacctc ttcggcggca ccatcgtcct ggccggcaag gtcgacctgg ctctcgggcg
     1081 ggccgagggg acccgggcgg gcaaggtgct gatcgacctc aagaccggac gatccagccc
     1141 gctccatcgt gaggacctgc gcttctacgc cctcatcgag acgctccggc tcggggtgcc
     1201 cccccggcgc atcgccaccc actacctcga ctcgggccat ctccagatcg aggacgtgag
     1261 cctcgatgtg ctggaggctg ccgtggcccg caccgtcgac gggatcgcgg cgatggtgga
     1321 gttgcgcgac cccgagcgcc cgctgcagaa gaagcctggg acggcgtgcg ggtggtgtgc
     1381 cttggccgag aactgcgccg agggtcagga gtggctgacc gacgagagtc gggcctactg
     1441 agcgctcaga ccggatcgat caggggttgg gccacctcgt cgtccaggtg ggccagcgag
     1501 ccgcgggccc gcaggaggac cagcacgaga acgaagcaga cgccgccggc agcgaccgtc
     1561 gcccggggtc cgatcacctc ggcgagggcg ccctggagca ggctgcccag cggtgcgcac
     1621 aaggtgatgc tgagcacgta gaaggcgagc accttgcccc gcatctgttc gtcgacctgg
     1681 agctggatgg tcgtgttcag gctggaggcg atcgtgaggt agcccgcgcc gacaccgagg
     1741 agaccgacga agccgacgta ggcgttgggt gcgagggcga agacggccat cgacgtgccg
     1801 cagaggagga tggccccggc gaccagcacc tggcgccgga acgtgctgcc ccacccggcg
     1861 acgaacggcg tggccaggat ggctccgatg ccgagcgccg cgctgaggac cccgaagacc
     1921 cactcgtcga cgacgaacac gtcctcggcg aagaccacca ccagttggaa gacggggctc
     1981 tcgaagaagc ccagcgcggc cacgacgatc acgcacgcgg cgatgcccgc cgacgcccgc
     2041 acatagcgga tcgtctcgcg gaagtcggcc acgatgtgca tccgcccgac ggccttctcc
     2101 accactcggt cgaggcgcat ggccaggagg gccgcgatga cggccaggaa gctcaccccg
     2161 ttgacgaaga aggcccatcc ggggccgacc agggcgagga tgacaccgcc gatcgccgga
     2221 ccgaaggccc gcgccgcgtt gaactgggcc gagttcagcg tcaccgcgtt gaggagcgcc
     2281 tcgcggggga cgagctcgga gacgaacgcc tgccacgagc cgatcatcag cccggcgaac
     2341 accccggagg tggccgccat ggcgatgatc acgcctgggg accggacgcc ggccccccaa
     2401 gccgcccaca gggccatcgc caccgcggcc tgggccgtgg acatgaccag cagcacggtg
     2461 cgccggtcga agcggtcggc cagggtcccg ccaacgggcc ccagtatcga ggccgtgatg
     2521 aggaggctga acgaggcgaa tcccacccat gccgcgctgt cggtcaggcg gaacacgacg
     2581 tagggcacgg tgacgttctg gacccagccc ccggtgttgg agacgacggc cccggtccag
     2641 aacagggcga agttccggta cctgaacgcg gccacggccg aacgaagtcc gggacggatc
     2701 tcctcttcga cgtcggcggg ttcagcagag ctcacggagg gccgaggcta cgagttggcc
     2761 ggcgcggtgc cgtccgcagc cggacgggca cgacaccgag gtgaacacct cgtttcggcc
     2821 gggtcggacc cgtttccgat cactcctcgc cggtagcctg cacccgcgta tggcctccga
     2881 ggacacccct gtggacacct cgcccgcggt cgccgaaggc ctgccggccg aggtccccgg
     2941 cgggaccctc gtccgtctgc ccatggaccg ctccgccagc ggccgcgagg acccggcccg
     3001 tcagggcgat gtcgatgagt gggggcgctc cgagcacatg cgggagctgg cccggaagct
     3061 ctacgacccc atctaccggt cctggttccg ggccgaatgg gaaggcctcg agaagatccc
     3121 ggagcacggc ggcgccctgc tggtggccaa ccatgccggt gccgtcccgt ccgacgcccc
     3181 cgtgatcatg cacggcatcg agagcgagct ggaacgaccc gtctacgggc tggccgacca
     3241 cctcttcaag tcgctgccgg tcgtcgggac gctgtggtcg cgggtcgggg gcgtggtggc
     3301 ccacccggac aacgcctatc gccttctcca cgacaagcgg gcgctcgtcc tggtgttccc
     3361 ggagggcacc aagggcaccg gaaagcacgt ctcggagcgc tatcgcctcc gtcgtttcgg
     3421 gcgcgggggc ttcgtcgaga tcgccatgaa ggccggggtc ccggtcatcc ccatcgccgt
     3481 ggtcggctcc gaggaggcga tgccgatcat cgccaagatc aatccgctgg ccaaggccct
     3541 caacctcccg tacgtgccca tcacccccca gatgctggcc ctcggcccgc tcgggctggt
     3601 ggcgccgctt cccgccaagt tccgcctccg ggtgctcgat ccgatccact tcgacgtccc
     3661 cgccggccag gagcgctaca gcaagagcaa ggtcatggac gccgcggacg acatccgcca
     3721 gcgcatccag gaccagctgt tcgacatgat ccgcacccgc cgttcgatct ggaagggctg
     3781 aggtgggtcg ccgcgttctc gtcacgggcc tgagcacgtt ctggggtggc ctcgtcgccc
     3841 agcagctcga ggccgacccc gacgtcgagg tcatcgtcgg gctggacacc tcgccccccg
     3901 agatcgagct ggaacgcacc gagttcgtcc gcgtggacca gaactactcc atcctcagcc
     3961 ggatcgtcgc ggcgaccaag gtcgacacga tcatccacac gttcctggtc atgaacaccg
     4021 gccaggtctc gtcccgggcc atgaaggaga tcaacgtcat cgggacgatg aacctgttcg
     4081 ccgccgcgtc ggcccccggc agcacggtcg agcgggtcgt ggtcaagtcc tcctcgctgg
     4141 tctacggcgc cgaccgggag gacccgtcgt tctttcgaga ggacatggcc cgcacccacc
     4201 acccgaagac cggcatcgag cgcagccttc tcgcggtcga ggactacgtc cgggacttcg
     4261 ccgtcgacaa cccccacgtg acggtgtcgc tgctgcgctt cagcaacgtc ctcgggccca
     4321 ccatctccac gccgttctcc aagctgctcc acctccccct ggtcccgtcg gtgttcgggt
     4381 tcgacccccg cttccagttc gtccacgagg acgacgtggt ccgctcgatc ctgttcgtgc
     4441 tcgacgagga cctggccggg atctacaacg tcgccggcga cgggatgctg ccctggagcg
     4501 aggtggccac catctgcggc aagcgcaccg tccccctgcc cccactggcc accggcgccg
     4561 cggccctgcc gctccgggcc ctcggctacg acatgccacc cgagacgctc gatctactgc
     4621 gctacggacg gggcgtggac ggcaccaagc tggtcgacgc cggcttccag taccgcttca
     4681 cctcggcggg cacagtcgag aacttcgccc aggccctccg actgcgatcg acggtcggcg
     4741 ctcgcgaccc ggagtacacc taccaggccg acgtggagca gttcttcaag cactccccgt
     4801 ccgtcgtgcg ggatgtgccc ctctcgtgag cgacgccgtc ctggtcgacg tcgtcgaccg
     4861 tgtggccacg gtcacgctca acgagccgga caagcgcaac gccatcagcc tgcccatggt
     4921 cggcggcatc gccgaggcct tcgatgccct cgaggccgac gaggccgtcg gtgccatcgt
     4981 tgtcaccggc gccccaccgg ccttctgtgc tggggccgac ctctcccacc tcggttcgtc
     5041 tcg
//
LOCUS       DASTFV010000548         3487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2040782, whole genome shotgun sequence.
ACCESSION   DASTFV010000548 DASTFV010000000
VERSION     DASTFV010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2040782"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1672)
                     /gene="dxs"
                     /locus_tag="VFP02_10055"
     CDS             complement(<1..1672)
                     /gene="dxs"
                     /locus_tag="VFP02_10055"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HET9543416.1"
                     /translation="MLEQIHSPADLKGLSPEELTDLAQEIREFVVTAVDEAGSGHLGS
                     NLGAVELTIALHRVFDSPDDIILWDTGHQAYVHKILTGRQSQFTELRKAGGLSGYPSR
                     EESPHDWIENSHASTVLSYAHGLAVAQESELGEGRRVIAVIGDGSMTGGMAFEGLNNF
                     GHSGRNAIIILNDNGRSYAPTISRLGESLARLRLNPTWLRQQARMERTLQRLPAGERM
                     VRGLEATKAAVREMWEPPAFFEQLGVRYTGPFDGHDIEGVERALRNAAELNGPVVVHV
                     LTQKGKGHAPAEQDSIKHMHDIGAVKPGSYTAAFTEALIKEADEHPELVAITAAMPDS
                     TGLLPFDERFPGRCFDVGIAEQHAVTSAAGMAMGGLRPVFAVYSTFLTRAIDQTFYDV
                     GLHRQPVVFCLDRAGVTGDDGPSHHGVLDMVLLRKVPGMTIFAPSSYQELGVMLHDAL
                     ELCTTGPSAIRWPKTAAPSVGPDEVGSGLHARLVRRGTDVCLIGVGKMLAPATEAAET
                     LEAEGLSVTVWDPRVVKPFDEALVEDALTHPVVVTVEDGLADGGVGSAL"
     gene            complement(1727..2473)
                     /locus_tag="VFP02_10060"
     CDS             complement(1727..2473)
                     /locus_tag="VFP02_10060"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9543417.1"
                     /translation="MNLRVGTFNIRNGVAFDGCNSWIFRRKATADMVSGLDADVVGLQ
                     EAYDFQARELRRRLEDYGLTGDGRSARRRGERCTVLFRQAALELTASETRWYGHEPTR
                     PGIQLPKASFPRVATIASFRPVDGGPPFTFVNTHLDERHEANRLTAVEQLLAWLDDGP
                     SIVVGDLNAEPDSRVLATLEAGGLRTVLPPDAPGTNHDFTGRTDGRRIDHILVSSHWE
                     IGPAQVVTGRPRGRLPSDHWPVVADLTLAV"
     gene            complement(2470..3051)
                     /locus_tag="VFP02_10065"
     CDS             complement(2470..3051)
                     /locus_tag="VFP02_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9543418.1"
                     /translation="MTRQRLVPVGAAAVLVVTGLLADARTPYALEGDIGRWVFDLPDL
                     LTTPLRAVMQVGGQLAIVLVAAALVVAGRYRAAGAAALAGLAGWLASSLLKDALDRPR
                     PTLATLGRVPRETVDNASWPSSHATIAFALATVLLLTVARGRVGRGLVIAAAVLTAVG
                     RVHLGVHWGLDVLGGAALGVLAGSLAVMIVKPT"
     gene            3093..3356
                     /locus_tag="VFP02_10070"
     CDS             3093..3356
                     /locus_tag="VFP02_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543419.1"
                     /translation="MCVNCVTGYEALALNSVAVITAGSSLWARLTTRVPREVTQRRVH
                     AENAALMRRLGLDPEAVLGPAPTVAADDPAPGPVERPQELVPT"
BASE COUNT          467 a         1202 c         1317 g          501 t
ORIGIN      
        1 ccagggcgga gcccacgccg ccgtcggcca gaccgtcctc gacagtgacc acgacggggt
       61 gggtcagggc gtcctcgacc agcgcctcgt cgaagggttt gaccaccctg gggtcccaga
      121 cggtcacgga caggccctcg gcctcgaggg tctcggccgc ctcggtggcg ggggccagca
      181 tcttgcccac accgatcagg cagacgtccg tgccccggcg aacgaggcgg gcgtgcaggc
      241 cggatccgac ctcgtcgggg ccgacgctgg gggcggccgt cttgggccag cggatggccg
      301 acgggccggt ggtgcacagc tccagggcgt cgtggagcat cacgcccagc tcctggtacg
      361 aggagggggc gaagatcgtc atccccggca ccttgcgcag caggaccatg tcgaggacgc
      421 cgtggtggga cgggccgtcg tcgccggtga cgcccgcccg gtcgaggcag aagacgacgg
      481 gctggcggtg caggccgacg tcgtagaagg tctggtcgat ggcccgggtc aggaaggtgg
      541 agtagacggc gaagacgggg cggagcccac ccatggccat gccggcggcc gacgtgacgg
      601 cgtgctgctc ggcgatgccg acgtcgaagc agcggccggg gaagcgttcg tcgaagggga
      661 gcagcccggt cgagtcgggc atggcggcgg tgatggccac cagctcgggg tgctcgtcgg
      721 cctccttgat gagggcctcg gtgaacgccg cggtgtacga ccccggcttc acggcgccga
      781 tgtcgtgcat gtgcttgatg gagtcctgct ccgcgggggc gtggcccttg cccttctggg
      841 tgagcacgtg gacgacgacg gggccgttca gctccgccgc gttgcgcagg gcccgctcga
      901 ccccttcgat gtcgtggccg tcgaaggggc ccgtgtagcg cacgcccagc tgctcgaaga
      961 aggcgggcgg ctcccacatc tcgcgcacgg cggccttggt ggcctcgagg ccccggacca
     1021 tccgctcgcc ggcggggagg cgttggaggg tgcgttccat gcgggcctgc tggcgcagcc
     1081 aggtcgggtt gaggcgcagg cgggccaggc tctcgcccag ccgggagatc gtcggggcgt
     1141 aggagcgccc gttgtcgttg aggatgatga tcgcgttgcg gccgctgtgg ccgaagttgt
     1201 tgaggccctc gaaggccatg cccccggtca tggagccgtc gccgatcacg gcgatgaccc
     1261 gccggccctc gcccagctcg ctctcctggg ccacggccag gccgtgggcg taggagagca
     1321 cggtcgaggc gtgactgttc tcgatccagt cgtgcggcga ctcttcccgg gaggggtagc
     1381 cggagaggcc gccggctttg cgcagctcgg tgaactggct ctggcgaccg gtcaggatct
     1441 tgtggacgta ggcctggtgg ccggtgtccc acaggatgat gtcgtcgggc gagtcgaaca
     1501 cccggtgcag ggcgatggtc agctcgacgg caccgaggtt ggacccgagg tgcccgctgc
     1561 cggcctcgtc cacggcggtg acgacgaact cccggatctc ctgggcgagg tccgtgagct
     1621 cctcggggga caggcccttg agatcagcgg gtgagtggat ttgctcgagc atgggtgcct
     1681 cggaaccgta ccgcttccgt caccggcgtc ggcagacgcc tggacctcag acggccagcg
     1741 tgaggtcggc caccaccggc cagtgatccg aaggcaggcg gccccggggc cgtccggtga
     1801 cgacctgggc cggaccgatc tcccagtggc tggacaccag gatgtggtcg atgcggcgcc
     1861 cgtcggtccg gccggtgaag tcgtggttgg tgcccggcgc atcggggggg aggaccgtgc
     1921 gcagcccgcc ggcctcgagg gtcgccagca cgcggctgtc gggctcggcg ttcaggtcgc
     1981 cgaccacgat gctggggccg tcgtccagcc aagccaggag ctgctccacc gccgtcagtc
     2041 ggttggcctc gtggcgctcg tcgaggtggg tgttgacgaa cgtgaacggc ggaccgccgt
     2101 ccaccggccg gaagctggcg atggtcgcca cccgggggaa cgacgccttc ggcagttgta
     2161 tcccgggccg ggtcggctcg tggccgtacc accgcgtctc gctggcggtg agctcgagcg
     2221 cggcctggcg gaagagcacg gtgcagcgct caccccggcg ccgcgccgag cgcccgtcgc
     2281 cggtcaggcc gtagtcctcg agccggcggc gcagctcccg ggcctggaag tcgtacgcct
     2341 cctggagccc gacgacgtcg gcgtcgagtc cgctgaccat gtccgccgtc gccttccgcc
     2401 ggaagatcca ggagttgcag ccgtcgaagg ccacaccgtt gcggatgttg aaggtgccga
     2461 cccggaggtt cacgtcggct tcacgatcat gacggcgagc gatccggcca ggaccccgag
     2521 ggcggccccg ccgagcacat ccaggcccca gtggacgccg aggtggaccc ggcccaccgc
     2581 ggtcagcacg gccgccgcga tcaccaggcc ccgcccgacc cgaccccggg ccacggtgag
     2641 caggaggacg gtggccaggg cgaaggcgat ggtggcgtgt gacgacggcc acgacgcgtt
     2701 gtcgacggtc tcgcggggga cgcggccgag cgtggccagg gtcgggcggg gccggtcgag
     2761 cgcgtccttg aggaggctgg acgccagcca gcccgccagc ccggccaacg ccgcggcccc
     2821 cgcggcccgg tagcgcccgg ccacgaccaa cgccgccgcg accaggacga tcgccaactg
     2881 gccgcccacc tgcatgaccg ctcggagggg cgtggtgagc aggtcgggga gatcgaagac
     2941 ccagcgcccg atgtcgccct cgagggcgta cggggtgcgg gcatcggcca gcaggccggt
     3001 gaccaccagc accgcggcgg caccgacggg caccaggcgc tgacgggtca cgcgctcagt
     3061 ctggccggtg gccgttgtta cgctccgcca tcgtgtgcgt gaactgcgtg accggctacg
     3121 aggccctcgc cctcaactcc gtcgccgtga tcacggccgg ctcctcgctc tgggcccgtc
     3181 tgacgacgcg agtgccccgt gaggtcacgc agcgccgggt ccacgccgag aacgcggccc
     3241 tgatgcgccg tctcggcctg gaccccgagg ccgtgctcgg ccccgcgccg acggtcgccg
     3301 ccgatgatcc ggccccgggc ccggtcgagc gtccgcagga gctggtcccc acatgatccg
     3361 ccgcttcctc cccttcgccg tcgccctggt ggtctacgtg atcctcggcg tgacgctgaa
     3421 gtcgttcttc ctcaactgga tcatcgggcc gctcttcctg ttgctggccc tcgacgtcct
     3481 gcctcgg
//
LOCUS       DASTFV010000549         3473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2041060, whole genome shotgun sequence.
ACCESSION   DASTFV010000549 DASTFV010000000
VERSION     DASTFV010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2041060"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VFP02_10075"
     CDS             complement(<1..662)
                     /locus_tag="VFP02_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HET9543420.1"
                     /translation="MPIELLTHIDQPSDSPARVDASSRPPFRIGTVQQRWHEDAAEHR
                     AALRDSVMMAADTGARLVCLQELTLSPYFAVTPDGPSAAGATPEPLPGGPTHDLATEL
                     ARDFDLFVHASLYEDAGDGGLGFNTAICVGPGGELVARTRKLHIPVTAGYYEDRYFRP
                     GDGGYPVVAVGDAHIGFPTCWDQWFPELARAYSLGGAEVIVYPTAIGSEPDHPDFDTQ
                     PL"
     gene            complement(662..1636)
                     /locus_tag="VFP02_10080"
     CDS             complement(662..1636)
                     /locus_tag="VFP02_10080"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HET9543421.1"
                     /translation="MTLAMPPEWAPHERCVVAWPCRADMWGGQLDAAKAAHAAVVNAI
                     VAFEPVLLVANPGAGAEAAVACPGAEVVEWPLDDSWARDIGAIVLTGDGRRAGLNPVF
                     NGWGEKFHPYDEDARFAVRMCEHLGLDRVDAFPFVLEGGAITVDGAGTVITTEQCLLN
                     PNRNPSLDRAGIEAELRKWLGVDRVVWLPYGLIDDDDTDGHVDNVALCVRPGVVLAQT
                     TTDPTHADHDRLRANGDVLVGAGYEVIEIDVLPEVVIDGQRADVPPLNLYLANGAAVV
                     PVVDGPEGDAALAAVRGALPEREVVGVPGDVLAFGGGGIHCITQQVPR"
     gene            complement(1663..2931)
                     /locus_tag="VFP02_10085"
     CDS             complement(1663..2931)
                     /locus_tag="VFP02_10085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9543422.1"
                     /translation="MPERLAAATRETFASFRVRNFRLFFLGQGISQIGNWMTLIAQTL
                     LVLDITHSGVAVGFLAACQFGPVLLLGAWAGLVADRSDKRHLLLIVQIVAMAQSFLLG
                     ALAFMDDPPLAALFAVASLGGLCMAFDNPARRAFVVEMVPEDLVHNAVSLNSAMMTSS
                     RVIGPALGGLLITTVGYGWAFWADGLSYIAVLVSLWLMRTEELRPSTPAVRGKGQVRQ
                     GLRYVRDTPVLAVPLAMMAVVGTLSFNFQVVLPLLVTRTFDRSKATFTILFSCISLGS
                     LVGALWTARRRSIGVIDVVKASLAFGVTMLALAVAPSIAWAFPIGAVMGMASIGFLTA
                     STAIVQTKAAPEMRGRVLALQAMVFLGSTPIGGPIVGYVSEHLGPRYAIAMGGFAGVG
                     AAAWGAMTLRGDRPEPAEPADLEVPVPETP"
     gene            complement(2988..>3473)
                     /locus_tag="VFP02_10090"
     CDS             complement(2988..>3473)
                     /locus_tag="VFP02_10090"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9543423.1"
                     /translation="AGGIGAGAITLVLTQKPVVRRIGRTLLTAVAVFGVATIALGFTT
                     SYWVAFAAMLVLSGADAISVFIRATLVPLVVPQEMRGRVLAVENVFIGGSNELGAFES
                     GVAGQILGPAGAVVLGGAATVAIAGTWAFAFPALRDIDRFPGSAQPVERDVIILDELG
                     P"
BASE COUNT          541 a         1165 c         1339 g          428 t
ORIGIN      
        1 cacaacggct gggtgtcgaa gtcggggtgg tcgggctcgg agccgatggc cgtggggtag
       61 acgatgacct cggccccgcc cagcgagtag gcccgggcca gctcgggaaa ccactggtcc
      121 cagcaggtcg ggaagccgat gtgggcgtca cccacggcga cgaccgggta gcccccgtcg
      181 ccgggccgga agtagcggtc ctcgtagtag cccgcggtga ccgggatgtg gagcttgcgg
      241 gtgcgggcca cgagctctcc gccggggccg acgcagatgg cggtgttgaa gccgaggccc
      301 ccgtcgccgg cgtcctcgta gagcgaggcg tggacgaaca ggtcgaaatc cctggccagc
      361 tcggtggcca ggtcgtgggt gggcccaccg gggaggggct cgggcgtggc gccggccgcc
      421 gacgggccgt cgggggtgac ggcgaagtag ggggagaggg tcagctcctg gaggcagacc
      481 agccgggcgc cggtgtcggc cgccatcatc acgctgtcgc gcagagcggc tcggtgctcg
      541 gccgcatcct cgtgccagcg ctgctgcacg gtgccgatcc ggaagggcgg ccgcgaagag
      601 gcatccaccc gggcgggcga gtccgacggc tggtcgatgt gggtcagcag ctcgatgggc
      661 atcagcgtgg cacctgttgg gtgatgcagt ggatcccgcc gccgccgaag gcgaggacgt
      721 cgccggggac accgaccacc tcgcgctcgg gcagggcgcc ccggaccgcg gcgagggcgg
      781 catcgccttc gggtccgtcg accacgggga cgacggcggc cccgttggcc aggtacaggt
      841 tcaggggcgg gacgtccgct cgctggccgt cgatgacgac ttccggcagg acgtcgatct
      901 cgatgacctc gtagccagcc ccgaccagca cgtcgccgtt ggcccgcagg cggtcgtggt
      961 cggcatgggt cgggtcggtc gtggtctggg ccagcacgac cccgggccgg acgcagaggg
     1021 ccacgttgtc gacgtgaccg tcggtgtcgt cgtcatcgat cagcccgtac ggcagccaga
     1081 ccacccggtc cacgccgagc cacttgcgga gctcggcctc gatgccggcc cggtccaggc
     1141 tcggattgcg gttggggttc agcaggcact gctcggtggt gatcaccgtg ccggccccgt
     1201 cgacggtgat ggccccgccc tccagcacga aggggaaggc gtcgacccgg tcgaggccga
     1261 ggtgctcaca catgcgcacg gcgaagcggg cgtcctcgtc gtaggggtgg aacttctcgc
     1321 cccacccgtt gaagaccggg ttcagcccgg cccggcgccc gtcaccggtc agcacgatgg
     1381 ctccgatgtc ccgggcccac gagtcgtcca gcggccactc gaccacctcg gcgcccgggc
     1441 aggccaccgc cgcctcggct ccggcgccgg ggttggcgac cagcagcacc ggctcgaagg
     1501 ccacgatggc gttcaccacg gcggcgtgtg cggccttcgc cgcgtccagc tgcccgcccc
     1561 acatgtcggc ccggcacggc cacgccacca cacaccgctc atggggcgcc cactccggcg
     1621 gcatggccaa cgtcatggcc acgactctcg cacagcgaag gcctagggcg tctcggggac
     1681 ggggacctcg aggtcggcgg gctcggcggg ttccggacgg tcgccgcgga gggtcatggc
     1741 tccccaggcg gcggccccca cgccggcgaa gccgcccatg gcgatggcgt agcgggggcc
     1801 gaggtgctcg gacacgtagc ccacgatggg gccgccgatg ggggtgctgc ccaggaagac
     1861 catggcctgg agggcgagga cccggccccg catctcgggg gcggccttcg tctggacgat
     1921 ggcggtcgag gcggtgagga agccgatgct ggccatgccc atgacggccc cgatggggaa
     1981 ggcccaggcg atgctcgggg cgacggcgag ggcgagcatg gtcacgccga aggccaagga
     2041 cgctttcacc acgtcgatca cgccgatcga ccggcggcgg gccgtccaca gggcgccgac
     2101 cagtgacccg aggctgatgc aggagaagag gatggtgaag gtggccttcg accggtcgaa
     2161 ggtccgggtc acgagcagcg ggaggacgac ctggaagttg aaggacaggg tcccgaccac
     2221 ggccatcatc gccaggggca cggcgagcac gggggtgtcc cgcacgtagc gcaggccctg
     2281 gcgcacctgg cccttgcccc ggacggccgg ggtcgacggg cgtagctcct cggtgcgcat
     2341 cagccacagg ctgacgagca cggcgatgta ggagagcccg tcggcccaga aggcccagcc
     2401 atagcccacg gtggtgatga gcagcccccc cagggcgggg ccgatgacgc gggagctggt
     2461 catcatggcg ctgttcaggc tgacggcgtt gtggacgagg tcttcgggga ccatctcgac
     2521 gacgaaggcc cgccgggccg ggttgtcgaa ggccatgcac agtccgccca gtgacgccac
     2581 ggcgaacagc gcggccagcg gcgggtcgtc catgaaggcc agggcgccga gcaggaacga
     2641 ctgcgccatg gccacgatct gcacgatgag caggaggtgg cgcttgtcgc tgcgatcggc
     2701 gaccaagccg gcccaggccc ccagcagcag gacggggccg aactggcagg cggccaggaa
     2761 gccgacggcg accccgctgt gggtgatgtc gaggaccagc agggtctggg cgatcagggt
     2821 catccagttc ccgatctggg agatgccctg gcccaggaag aacagccgga agttgcgcac
     2881 ccggaacgac gcgaacgtct cccgcgtggc agcagcgagt cgttccggca ttgggatcga
     2941 ccctacgggg ccacccttct gagcggaacg cccctcgccc gaagggccta cgggccgagc
     3001 tcgtccagga tgatgacgtc ccgctcgacg ggttgtgcgc tgccggggaa gcggtcgatg
     3061 tcacggaggg cggggaaggc gaaggcccag gtcccggcga tggcaaccgt cgccgccccg
     3121 ccgaggacca cggccccggc cgggccgagg atctggccgg cgacgccgga ctcgaaggcg
     3181 ccgagctcgt tggacccccc gatgaagacg ttctcgacgg ccaacacccg gccccgcatc
     3241 tcctggggga cgacgagggg cacgagggtg gcccggatga acacgctgat ggcgtcggcc
     3301 ccggagagca cgagcatggc ggcgaaggcc acccagtagg acgtggtgaa cccgagggcg
     3361 atggtggcca cgccgaacac ggcgacggcg gtcaggaggg tgcgcccgat gcggcggacg
     3421 accggcttct gggtcaggac gagggtgatg gcgcccgcgc cgatgccgcc cgc
//
LOCUS       DASTFV010000550         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2042643, whole genome shotgun sequence.
ACCESSION   DASTFV010000550 DASTFV010000000
VERSION     DASTFV010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2042643"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /gene="rpmF"
                     /locus_tag="VFP02_10095"
     CDS             complement(<1..153)
                     /gene="rpmF"
                     /locus_tag="VFP02_10095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET9543424.1"
                     /translation="MAVPKKKTSKMKGRSRMASAWRIKTTGRSTCPRCDAVRRPHVVC
                     SNCGWYG"
     gene            complement(314..811)
                     /locus_tag="VFP02_10100"
     CDS             complement(314..811)
                     /locus_tag="VFP02_10100"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HET9543425.1"
                     /translation="MAARLRVGVADLLRNLGTRREFRDQIRIDDLGVVSTRVDPDADV
                     DLEFFIEAFSGGISVTGRIEVPWTGECRRCLDEVRGTTVQHVQEIFGGPEGDEDAYPL
                     GHDEIDLEPMVRDVVAGALPMVPLCRPDCAGPSPDRFPTGPADESAPRGDDRWAALDE
                     LSFDE"
     gene            complement(817..1395)
                     /locus_tag="VFP02_10105"
     CDS             complement(817..1395)
                     /locus_tag="VFP02_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543426.1"
                     /translation="MSATEPTAKSGGYKMPEVEQLLDRVTDLIDQARPMPLSTSAMIN
                     KDEILDLLVEVRSRLPEELRASRWLLKEREEFLEKVKREGEDLLEEARSQAERMVQRT
                     EVVKAAEQRGRQIVEKAETRARQMRNEAEDFVDQKLGSFEIALERVQAQVASGREKLQ
                     AVATPNAEDNLADVAQFGAHGDERTEFFDQEG"
     gene            complement(1392..1868)
                     /gene="coaD"
                     /locus_tag="VFP02_10110"
     CDS             complement(1392..1868)
                     /gene="coaD"
                     /locus_tag="VFP02_10110"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET9543427.1"
                     /translation="MTKVLCPGSFDPIHNGHLEVIERAAHLFDEVVVAAMRNPQKGEP
                     LFSLEEREAMISESLSHLDNVTFTMFSSLVVDLARDIGADFIVKGLRAVSDFESELQM
                     AQMNHAISGVDTLFIPSASSHSFLASKLIREIARFGGDVDSMVPPPVAKRLAERLT"
     gene            1925..2119
                     /locus_tag="VFP02_10115"
     CDS             1925..2119
                     /locus_tag="VFP02_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543428.1"
                     /translation="MTDPFPLVPETIVCVDCGGVCHLLSHPPPDEPFGPGDVVAYRCS
                     DCMDRWDIVIPDEDDARGDD"
     gene            2225..>2625
                     /locus_tag="VFP02_10120"
     CDS             2225..>2625
                     /locus_tag="VFP02_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543429.1"
                     /translation="MIRRLVVAAAVVAATATPRPADAADERPRFTACAAVSATAPRCH
                     TGGVTYEVGRTVFLRGRVTPARPGFGEVVRRAPRGGRFVPVGTMGIGTDGRIRWAWTP
                     RRRDVNRAEPYLFAFRIAGVGRSTAVEVWVVP"
BASE COUNT          358 a          929 c          915 g          423 t
ORIGIN      
        1 gccgtaccaa ccgcagttgc tgcacaccac gtgcgggcga cgcacggcat cacaacgcgg
       61 gcacgtgctg cgcccggtcg tcttgatgcg ccaggcggaa gccatgcggc tccggccctt
      121 catcttggag gtcttcttct tggggacggc catcgtcagg tcctcgagag tttcagtgcc
      181 aacacggtgg tgaagcgacg ggtcagggta gcggcgagac cgtcacactt gcgttcccgc
      241 gctgcgctca cgaacgccag cgccgccgat cctgagggtc ctccgagctg ctccgctccg
      301 ctgcgcagcg tgcctactca tcgaagctga gctcgtcgag agcggcccag cggtcgtcgc
      361 cccggggggc ggactcgtcg gcggggccgg tcgggaaacg gtcgggcgag gggccggcgc
      421 agtcgggccg gcagaggggg accatcggga gggcgccggc gacgacgtcg cggaccatgg
      481 gctcgaggtc gatctcgtcg tggccgaggg ggtaggcgtc ctcgtcgccc tcgggcccgc
      541 cgaagatctc ctggacgtgc tggacggtcg tgccccgcac ctcgtcgagg cagcggcggc
      601 actccccggt ccacggcacc tcgatgcgcc cggtcaccga gatgccgccg ctgaaggcct
      661 cgatgaagaa ctcgaggtcc acgtcggcat ccgggtccac ccgagtggag accacgccca
      721 ggtcgtcgat gcggatctgg tcgcggaact cgcggcgggt cccgaggttg cgcagcaggt
      781 cggccacgcc gacccgcagg cgggcggcca ccgggctcag ccctcttggt cgaagaactc
      841 ggtgcgctcg tcgccgtggg caccgaactg ggccacgtcg gcgaggttgt cctccgcatt
      901 cggggtggcc acggcttgca gcttctcccg gcccgaggcg acctgggcct gcacccgctc
      961 gagagcgatc tcgaagctcc ccagcttctg gtcgacgaag tcctccgctt cgttgcgcat
     1021 ctggcgggcc cgggtctcgg ccttctcgac gatctggcgg ccccgctgct cggccgcctt
     1081 gaccacctcg gtgcgctgca ccatgcgctc ggcctggctg cgggcctcct cgagcaggtc
     1141 ctcgccctcg cgcttgacct tctccaggaa ctcctcgcgc tccttgagga gccagcggga
     1201 cgctcgcagc tcctccggga ggcgggagcg gacctcgacc agcaggtcga ggatctcgtc
     1261 cttgttgatc atcgccgacg tcgacagggg catcggccgg gcctggtcga tgaggtcggt
     1321 cacccggtcg agcagctgct cgacctcggg catcttgtac ccgccggact tggcggtcgg
     1381 ctcggtggcg ctcacgtgag tcgctccgcg aggcgcttgg ccacgggggg cgggaccatg
     1441 gagtccacgt cgccgccgaa gcgggcgatc tcgcggatca gcttggacgc caggaaggaa
     1501 tgggacgacg ccgacgggat gaacagcgtg tccacccccg agatggcgtg attcatctgg
     1561 gccatctgca actcactctc gaagtccgag accgcccgca accccttgac gatgaagtct
     1621 gccccgatat cccgggccag gtccaccacc aggctggaga acatggtgaa ggtgacgttg
     1681 tcgaggtgcg agaggctctc gctgatcatc gcctcccgct cctcgaggga gaacagcggc
     1741 tcgcccttct gcgggttgcg catggccgcg accacgacct cgtcgaagag atgggcggcc
     1801 cgttcgatga cctcgagatg accgttgtgg atggggtcga acgacccggg gcagagcacc
     1861 ttcgtcacga cggggacggt accgggtccc ccgcccgccg acccaccggc gtaccgtcgg
     1921 ggtggtgacc gaccccttcc ccctggtgcc cgagaccatc gtgtgcgtcg actgcggggg
     1981 cgtctgccat ctgctctcgc acccaccacc cgacgagccc ttcggccccg gcgacgtcgt
     2041 ggcctaccgc tgctcggact gcatggaccg ctgggacatc gtcatccccg acgaggacga
     2101 cgctcgcggc gacgactgac gggttcccgc gacatcttct cgtcatttcc ataattttct
     2161 gttgatttcc atcaccgaga tgggggaggc tctcgggtga gggggctcga ccgaggagct
     2221 ccagatgatc cgacgtctcg tcgtcgccgc cgcggtcgtc gccgcgacgg ccaccccccg
     2281 cccggcagat gcggccgacg agcggccccg gttcacggcg tgcgccgcgg tgtcggcgac
     2341 ggcgccccgc tgtcacaccg gcggcgtcac ctacgaggtc ggtcgcacgg tgttcctccg
     2401 cggtcgggtc acaccggccc gcccggggtt cggcgaggtg gtgcgtcgcg ctccccgagg
     2461 cggccggttc gttccggtcg gcacgatggg catcgggacc gacggccgca tccgctgggc
     2521 gtggacgccc cggcggcgcg acgtcaaccg cgccgagccc tacctgttcg ccttccgcat
     2581 cgccggggtc ggccgcagca cggcggtcga ggtctgggtc gtccc
//
LOCUS       DASTFV010000551         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2042892, whole genome shotgun sequence.
ACCESSION   DASTFV010000551 DASTFV010000000
VERSION     DASTFV010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2042892"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(531..1481)
                     /locus_tag="VFP02_10125"
     CDS             complement(531..1481)
                     /locus_tag="VFP02_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET9543430.1"
                     /translation="MTALPLPRARLAGPPAGLFYLLLVVVGLAAFLYPFWIPAETAFA
                     EAHAADAPFVAAGVGLLAVLAIALEVRRGTTTGATIAVLAVLSAMAGVLRLVDLPGGG
                     SGIFFLIVLAGAAYGPRFGLLLGLGAMSTSALITGGMGPWLPFQMLALAWMGAGAGFV
                     GRLTRRLPVPAEVVILAGYGWVWGFLYGAIMNIWFWPYLAGQGALAWEPGLGFYGTLE
                     RYWHFYTVTSLAWDAAAAMTNAALILLTGRVALRTFRRFEARLVGPEEFGSDRRWAAA
                     HDPDPPADADHHDEDQHDERGGEGQSTALGGTGVAGPAQG"
     gene            complement(1478..>2094)
                     /locus_tag="VFP02_10130"
     CDS             complement(1478..>2094)
                     /locus_tag="VFP02_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9543431.1"
                     /translation="ALLGRNGSGKTTLLRALAGLLEVDGGNVDAWARIAYLPQDPNAL
                     LFAPTVRAELEHTLRLLARRDPEAVDTWLHRLGLTALADRHPRSLSAGQRQRVAIGAV
                     AVGGAEVLLLDEPTRGIDPPSAAALEHAVADHAAGGGAVVLATHDVELAARCATRAVV
                     LGDGDVVASGAARDVLAGSLFAPQVLRVLPPYLTVAEVAAALDR"
BASE COUNT          321 a          760 c          793 g          220 t
ORIGIN      
        1 cgtcggactg gctggaggac gacgacaccc ccttcgatgc ccgcgtcgag ctggccatcg
       61 ccctgattgc ggtcggagcc gagctgcccg agagcctcgt cgacgaggtc ctggcggccc
      121 agcaggccaa cggcggttgg aacgccgacg gcgacgccga cgccgacacc gtcgacctgg
      181 tcacgaccgg cgccgcggtc gacctcctgg tcctcgtcgg cgccgacacg gcgagcggtg
      241 cgatccccgc ggccatggcc ttcgtcggcg agacccgcgc tcgcaacggg gcctggcccg
      301 accccgaagg cgaacgctcg gcggtggcta cggccggggc gatccgagcc atccgggccg
      361 acggccacga ccccgccggc agctgctggc tgaccgaggt cggcgtcgac gccgatgcca
      421 cggctccggt tgcggccctc atcgctctcc agaacgacaa cggcagcttc agcggtgaca
      481 acccggtgcg agcgaccggc gacgccgtgc acgcgctcag tgggcgctgg ctacccctgg
      541 gccgggccgg cgacgcctgt gcccccgaga gcggtggatt gcccttcgcc ccctcgctca
      601 tcgtgctggt cctcatcgtg gtggtcggcg tcggcggggg ggtccggatc atgcgcggcg
      661 gcccatcggc ggtctgaccc gaactcctcc gggcccacga gccgcgcttc gaagcggcgg
      721 aacgtgcgca gggcgacccg acccgtcagc aggatgaggg cggcgttggt catcgcggcc
      781 gccgcgtccc aggccaacga ggtcaccgtg tagaagtgcc agtagcgctc gagcgtgccg
      841 tagaacccca ggccaggctc ccaggccaga gcgccctgac cggccaggta cggccagaac
      901 cagatgttca tgatggctcc gtacaagaag ccccagaccc agccgtagcc ggccaggatc
      961 acgacttcgg cggggaccgg taggcggcgg gtcaagcgcc cgacgaagcc ggcgcccgcg
     1021 cccatccagg ccaaggccag catctggaac gggagccacg gtcccatgcc gccggtgatg
     1081 agggcggagg tgctcatcgc tcccagcccc aggaggagcc cgaagcgcgg gccgtacgcc
     1141 gctccggcca ggacgatcag gaagaagatc cccgagcccc caccgggcag gtcgacgagg
     1201 cggagcaccc cggccatcgc cgagagcacg gcgagcaccg cgatcgtggc cccggtggtc
     1261 gttccgcggc ggacctcgag ggcgatggcc aacacggcca ggaggccgac cccggcggcg
     1321 acgaagggcg cgtcggcggc gtgggcttcg gcgaaggccg tctcggcggg gatccagaac
     1381 ggatagagga aggccgccag accgacgacc acgagcagca ggtagaagag gccggcgggt
     1441 ggcccggcca ggcgggcccg ggggagcggg agggcggtca tcggtccagc gccgccgcca
     1501 cctcggcgac ggtgaggtag gggggcagca cccggaggac ctgcggagcg aacagcgagc
     1561 cggccaggac gtcccgggcc gcccccgaag ccaccacgtc accgtcgccg aggaccacgg
     1621 cccgagtggc gcagcgggcc gccagctcga cgtcgtgggt ggccaggacc accgcaccac
     1681 cgccggcggc gtggtcggcc acggcgtgct ccagggcggc cgccgacggc gggtcgatgc
     1741 cccgggtggg ctcgtccagc aggaggacct cggccccgcc caccgccacc gccccgatgg
     1801 cgacgcgctg gcgctggccc gccgacagtg accgcgggtg gcggtcggcc agggccgtga
     1861 ggccgaggcg gtgcagccag gtgtcgacgg cctcgggatc ccggcgggcc agcagccgaa
     1921 gcgtgtgctc cagctcggcc cggacggtgg gggcgaacag aagggcgttg ggatcctggg
     1981 ggaggtaggc gatccgggcc caggcgtcga cgttgccacc gtcgacctcg aggagcccgg
     2041 ccaacgcccg cagcaacgtc gtcttgccgg agccgttccg gcccaggaga gcca
//
LOCUS       DASTFV010000552         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2058146, whole genome shotgun sequence.
ACCESSION   DASTFV010000552 DASTFV010000000
VERSION     DASTFV010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2058146"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VFP02_10135"
     CDS             complement(<1..564)
                     /locus_tag="VFP02_10135"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET9543432.1"
                     /translation="MATPTRVAVTGAAGQIGYSLLFRIANGDLLGPDRPVRLQLLEIP
                     PALGALQGVAMELDDCAFPLLDSIVQTDQPEVAFDGADVALLVGSRPRTKGMERADLL
                     SANGAIFTVQGRAIASSAKADVKVLVVGNPANTNCLIAMNNAPGLARSAFTAMTRLDH
                     NRAVAQLAARAAAAVGDVNHMTIWGNHS"
     gene            complement(615..>2077)
                     /locus_tag="VFP02_10140"
     CDS             complement(615..>2077)
                     /locus_tag="VFP02_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9543433.1"
                     /translation="HVATAEVADIEVESSGDEATAAFAADLGLQGLGQWAYEGTVDLQ
                     RVDGTWGVAFTPASLHPALTDGARLQRVREQGPRAVITAAGGQPLAVGGPGAGSRLDG
                     LAGSLIGGLRELTAPEAAQLGLDYAAGDLVGDDGVESGYEGRLDGDPVGRIEVVDAAG
                     RVTAVLEEFGGEAPPPLELTIDYATQRAAEAAIANLTLPTALVAVDSRTGAIRAVANN
                     PPGFDRALLGDYAPGSTFKIVTASALLGAGLERDQIVPCPYEIRPGDSAPFTNAFGED
                     YGDIPFIEAFAKSCNTTFVSQGFQLGASRLHEMAETFGFNQAYDIGVPVAAPSFPLPE
                     SDTELGAASIGQGRVSVTPLHMATVAAAAYDGTWRPPYLAGEPPTQGSHPLPAAVAAA
                     LPDFLREVVRSGTGTGAAIIGRDVGGKTGTAEFGEEDPPETHAWFAGFIDDLAFAVVV
                     EGGGIGGEVAAPIVHEFLVNLPAPPVGPGALPEAGG"
BASE COUNT          281 a          714 c          806 g          276 t
ORIGIN      
        1 ggagtggtta ccccagatcg tcatgtggtt cacgtcgccg accgcggcgg cggcccgagc
       61 ggccagctgg gccacggcgc ggttgtggtc gaggcgggtc atggccgtga aggcgctgcg
      121 cgccagcccc ggcgcgttgt tcatggcgat gaggcagttg gtgttggcgg ggttgccgac
      181 gaccagcacc ttcacgtcgg ccttggcgct ggaggcgatg gctcgaccct gcacggtgaa
      241 gatggccccg ttggccgaca ggagatcggc ccgttccatg cccttggtgc ggggccggga
      301 gccgaccagg agagccacat cggcgccgtc gaaggcgacc tcgggctggt cggtctggac
      361 gatgctgtcg agcagcggga aggcgcagtc gtcgagctcc atggccacgc cctggagggc
      421 ccccaacgcg ggagggatct ccagcagctg caggcgtacg ggtcgatccg ggccgagcag
      481 gtcgccgttg gcgatgcgga acaggaggct gtagccgatc tggccggcag cgccggtcac
      541 ggccacgcga gtgggggttg ccatgggcgg accctaccga gagggtcgct gaccgatcta
      601 gccgacgaat cggctcagcc gccggcctcg ggcagggcgc cggggccgac cggcggggcg
      661 ggcaggttga cgaggaactc gtggacgatc ggggccgcca cctccccgcc gatcccgccg
      721 ccctcgacga ccacggcgaa ggccaggtcg tcgatgaagc cggcgaacca ggcgtgggtc
      781 tccggcgggt cctcttcgcc gaactcggcg gtgcccgtct tgcccccgac gtcccgaccg
      841 atgatggccg cgccggtgcc cgtgcccgac cgcacgacct cccgcaagaa gtcgggtagg
      901 gccgcggcga cggcggcggg gagcggatgg ctgccctggg tgggcggctc gccggcgagg
      961 tacggcggcc gccacgtccc gtcgtaggcc gcggcggcga ccgtggccat gtgcagcggc
     1021 gtgaccgaca ctcgaccctg gccgatcgaa gcggccccga gctcggtgtc ggactccggg
     1081 agcgggaacg agggggccgc cacaggcacg ccgatgtcgt aggcctggtt gaacccgaag
     1141 gtctcggcca tctcgtgcag ccgggaggcc ccgagctgga agccctggct gacgaaggtg
     1201 gtgttgcagg acttggcgaa ggcctcgatg aaggggatgt cgccgtagtc ctcgccgaag
     1261 gcattggtga acggggccga gtccccgggc cggatctcgt aggggcaggg cacgatctga
     1321 tctcgctcga ggcccgcgcc gagcagggcc gaggcggtga cgatcttgaa cgtcgatccc
     1381 ggggcgtagt cgcccaggag ggcccggtcg aagcccgggg ggttgttggc cacggcccgg
     1441 atggcgcccg tccggctgtc gacggcgacc agagcggtgg gcagggtcag gttcgcgatc
     1501 gcagcttcgg ccgctcgctg cgtggcgtag tcgatggtga gctccagggg cgggggggcc
     1561 tcaccgccga actcctcgag gacggcggtg acccggcccg cggcatcgac cacctcgatg
     1621 cggcccaccg ggtcgccgtc gagccggccc tcgtacccgg actccacgcc gtcgtcaccg
     1681 accaggtcgc ccgcggcgta gtcgaggccg agctgggccg cctccggcgc ggtcagttcc
     1741 cgcaggccac cgatcaggga gccggccaac ccgtccagac ggcttccggc cccgggtccg
     1801 ccgaccgcca ggggctggcc cccggccgcg gtgatgaccg cgcgcggccc ctgctcccgc
     1861 acccgttgga gccgggcgcc gtcggtcagg gccgggtgga gggatgccgg cgtgaacgcc
     1921 acgccccagg tgccgtccac ccgctgcagg tcgacggtgc cctcataggc ccactgcccg
     1981 aggccttgga ggccgaggtc ggcggcgaag gcagcggtgg cctcgtcccc cgacgactcg
     2041 acctcgatgt cggccacctc ggccgtggcc acgtgca
//
LOCUS       DASTFV010000553         2749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2059396, whole genome shotgun sequence.
ACCESSION   DASTFV010000553 DASTFV010000000
VERSION     DASTFV010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2059396"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..119)
                     /locus_tag="VFP02_10145"
     tRNA            complement(48..119)
                     /locus_tag="VFP02_10145"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(85..87),aa:Val,seq:cac)
     gene            complement(172..552)
                     /locus_tag="VFP02_10150"
     CDS             complement(172..552)
                     /locus_tag="VFP02_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9543434.1"
                     /translation="MVQVTGFDHIVLNVADVEISAAWYQDKLGLELIRMEEWRRGEVL
                     FPSLRIDATTLIDLFRSERTGENLNHFAVVVDADDLQAVIDSGEFDLAGPPQPSDLFG
                     AQGQGLGIYVKDPDGNTVELRTYP"
     gene            complement(563..1453)
                     /gene="ligD"
                     /locus_tag="VFP02_10155"
     CDS             complement(563..1453)
                     /gene="ligD"
                     /locus_tag="VFP02_10155"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET9543435.1"
                     /translation="MPVVEIDGRELSVTNLDKVLYPDGFTKAEVIDYYVRVAPAMLPH
                     VQGRGVTLRRYPNGVDGTSFFEKRSPDHRPDWIGTALGPGDRKGGIQYLVIDEVATLA
                     WVGNMAALEVHAPMARAVDIDAPTMVVFDLDPGAPATIVDCCRVALEIQEVLADLELQ
                     IWPKTSGSKGMQLYLPLNTPHTHEHASSFALAVAQVLENQDPKRVTSVMKKQLRKGKI
                     FVDWSQNSHHKTTIAAYSLRARPTPTVSTPISWDEVQSGADGEVLSFTAPEVLERVAD
                     FGDVFAPTATLEQELPTPRS"
     gene            complement(1460..2329)
                     /locus_tag="VFP02_10160"
     CDS             complement(1460..2329)
                     /locus_tag="VFP02_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET9543436.1"
                     /translation="MPRAIWSGSISFGLVNIPVQLFNAVSRKSVRFNQIDSRNNARIR
                     MKKTNAESGEDVPDDVIVKGYELSKGNYVLLSEDELAALDPESQRTIDIEEFVDLADI
                     DPVYFDASYHLSPDPATVKAYALLARTMEQAGKVGISRFVMRQKQYLAAIRPVDGHLV
                     LSTLVYEDEVNGVDEVPELDKVSEVDVSAKELEMAEQLVDSLAGTWEPEKYSDTYREQ
                     VMEMIEAKAAGDTDFVVATEAPPREKVVDLMAALEASVKDAKEARGRHPTSGEAETKK
                     KPARKAPAKKKSA"
     gene            2368..>2749
                     /locus_tag="VFP02_10165"
     CDS             2368..>2749
                     /locus_tag="VFP02_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543437.1"
                     /translation="MPRSPASNTRDTGAIALAAASVISGVFAYLFIAIGTRQFGTGDF
                     SGVAQLWTIWFFGSSVLTFPIQHWVIQRLRTDGHGGAVRVVLPRLSLFALGISLAVLA
                     ATYLVRGDMYGTDEIAYPIIAGGIT"
BASE COUNT          390 a          915 c          949 g          495 t
ORIGIN      
        1 ccgcatgagt agacccaatt cggcgtctga ccaggacatt cacagagtgg gcgatgaggg
       61 tgtcgaaccc tccgcctctg ccgtgtgaag gcagcgctct accgctgagc taatcgcccg
      121 attgggagca cgagcgtagc gccgacccct cggtcaccgt tcccggcgag gtcaggggta
      181 ggtgcggagc tcgacggtgt tgccgtcggg gtccttgacg tagatgccga ggccctggcc
      241 ctgggcgccg aacaggtcgc tgggctgcgg cgggccggcc aggtcgaact cgccggagtc
      301 gatgaccgcc tgcaggtcgt cggcgtccac gacgacggcg aagtggttca ggttctcacc
      361 cgtccgctcg ctccggaaga ggtcgatgag cgtggtggcg tcgatgcgca gcgaggggaa
      421 caggacctcg ccccggcgcc actcctccat gcggatcagc tccaggccga gcttgtcctg
      481 gtaccaagcc gccgagatct cgacgtcggc cacgttcagg acgatgtgat cgaatccggt
      541 cacctgcacc atggcgtcga gcctaggagc ggggcgtggg gagctcctgc tcgagggtgg
      601 cggtcggggc gaacacatcg ccgaagtcgg ccacccgctc caggacctcc ggggcggtga
      661 acgacaggac ctcgccatcg gcgccggact ggacctcgtc ccagctgatc ggggtcgaga
      721 cggtcggggt gggccgggcc cgcagcgagt aggcggcgat cgtcgtcttg tggtgggagt
      781 tctggctcca gtccacgaag atcttcccct tccggagctg tttcttcatg acgctggtga
      841 cccgcttcgg gtcctggttc tccagcacct gggccaccgc cagggcgaag gacgaggcgt
      901 gctcgtgggt gtgcggggtg ttgagcggca ggtagagctg catccccttg gagccggagg
      961 tcttgggcca gatctgcagc tccaggtcgg cgagcacttc ctggatctcg agggccactc
     1021 ggcagcagtc gacgatggtc gccggcgcgc ccgggtcgag gtcgaagacc accatcgtcg
     1081 gcgcgtcgat gtcgaccgcc cgggccatcg gggcgtggac ctcgagggcg gccatgttcc
     1141 cgacccaggc cagcgtggcc acctcgtcga tcaccaggta ctggatccca cccttgcggt
     1201 cgccgggtcc gagcgcggtg ccgatccagt cggggcggtg atccggtgac cgcttctcga
     1261 agaacgaggt cccgtccacg ccgttcggat accggcgcag ggtcacgccc cgcccctgga
     1321 cgtgcggcag catcgccggc gcgacccgga cgtagtagtc gatgacctcg gccttggtga
     1381 acccgtccgg gtagaggacc ttgtcgaggt tggtgaccga cagctcccga ccgtcgatct
     1441 cgacgacggg cacgggccgc taggccgact tcttcttggc cggagccttc cgcgcgggct
     1501 tcttcttcgt ctcggcctca ccggaggtgg gatgccggcc gcgggcctcc ttggcgtcct
     1561 tgacgctggc ctcgagcgcc gccatgaggt ccacgacctt ctcccggggc ggagcctcgg
     1621 tggccacgac gaagtcggtg tcgcccgccg ccttggcctc gatcatctcc atgacctgtt
     1681 cccggtaggt gtcgctgtac ttctcgggct cccaggtgcc ggcgagcgag tcgaccagct
     1741 gctcggccat ctccagctcc ttggcagaga cgtcgacctc gctgaccttg tccagctccg
     1801 ggacctcgtc gaccccgttg acctcgtcct cgtacaccag ggtggagagg accaggtggc
     1861 cgtcgacggg gcggatggcg gccaggtact gcttctggcg catcacgaag cgggaaatgc
     1921 cgaccttgcc cgcttgttcc atggtgcggg ctaacagggc gtaggccttc accgtggccg
     1981 ggtccggcga caggtggtac gaggcgtcga agtagaccgg gtcgatgtcg gccaggtcga
     2041 cgaactcctc gatgtcgatc gtgcgttggg actcggggtc gagggcggcc agctcgtcct
     2101 cggagagcag cacgtagttg cccttggaca gctcgtagcc cttgacgatg acgtcgtcgg
     2161 gcacgtcctc gccgctctcc gcattggtct tcttcatgcg gatccgggcg ttgttgcgcg
     2221 agtcgatctg gttgaaccgg accgacttgc gactgacggc gttgaagagc tggacgggga
     2281 tgttgaccag gccgaagctg atcgacccgc tccagatggc acgcggcatg ggtccagtat
     2341 ggcggcacct gcctacgctc ggccgccgtg ccccgttcgc ctgcctcgaa cacccgggat
     2401 acgggggcga tcgccctggc cgcggccagc gtcatcagcg gcgtcttcgc ctacctgttc
     2461 atcgccatcg ggacccggca gttcggcacc ggcgacttct ccggcgtcgc ccagctgtgg
     2521 acgatctggt tcttcggctc gtccgtcctg accttcccca tccagcactg ggtcatccaa
     2581 cgcctgcgca ccgacggcca cggcggggcg gtccgggtcg tgctcccccg cctgagcttg
     2641 ttcgccctcg ggatcagcct ggccgtcctg gctgccacct atctcgtccg gggggacatg
     2701 tatgggactg acgaaatcgc gtacccgatc atcgccggcg gcatcacgg
//
LOCUS       DASTFV010000554         2171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2064393, whole genome shotgun sequence.
ACCESSION   DASTFV010000554 DASTFV010000000
VERSION     DASTFV010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2064393"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="VFP02_10170"
     CDS             complement(<1..1159)
                     /locus_tag="VFP02_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET9543438.1"
                     /translation="MAPFPSDFLWGSSTAGHQVEGGNVNADLWPLEWAEGSQFVEPSL
                     DACDHYHRYAEDIATLAELGLNAYRFSVEWSRIEPEPGAFSRAALDHYRRMVGTCLEH
                     GVTPVVTYCHFTTPRWFAGAGGWAAADAPDRFGRYAERVTEHLGDLLPWVATLNEPNV
                     LDLMQQTGAIPMGVGQSGPVQEVEAPAPAGVGGFDPARYRMGLVGAGIEQMAAIHRRA
                     VEAIKAGPGDAKVGWTLALVDLQPAEGGEARWEQARQGASLDWLEVSRDDDFVGVQTY
                     TRERIGPEGKIALPEGVPTMQTGWEVYPEALEHTVRMAAEHAGVPVVVTENGMATDDD
                     EARIAYTRGALEGLARCVEDGLDVCGYLHWTFLDNFEWTAGFAKTFGLVAFD"
     gene            complement(1201..1983)
                     /locus_tag="VFP02_10175"
     CDS             complement(1201..1983)
                     /locus_tag="VFP02_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543439.1"
                     /translation="MDLRAGDVAVTVDPARGGRVASLRVAGHELLIAEPEAGDPKLWG
                     CYPMVPWAGRVRDGHFAFRGTTHELPLDAPPHAIHGVGYRTPWQETGPGSLRLDLDGH
                     WPFGGTVTQDVALTPTSLTVTMTVTAAEQAMPLVMGWHPCFRRRLDVGEAAQLTVPAT
                     SMWERDASGIPTGQQVPVPAGPWDDAFGGLRADPRITWPGAVEVTLRSSCPVWVVYDQ
                     DPRLVCVEPQTAAPDAFNRDPKVVEPGRSLRAHLEISWSSLT"
BASE COUNT          303 a          770 c          802 g          296 t
ORIGIN      
        1 ggtcgaaggc gaccaggccg aaggtcttgg cgaatcccgc cgtccactcg aagttgtcga
       61 ggaaggtcca gtgcaggtac ccgcacacgt cgaggccgtc ctcgacgcag cgggccagcc
      121 cctccagggc gccccgggtg taggcgatgc gggcctcgtc gtcgtcggtc gccatgccgt
      181 tctcggtcac cacgacgggc acgccggcgt gctcggcggc catccgcacg gtgtgctcga
      241 gggcctcggg gtagacctcc cacccggtct gcatggtggg gacgccctcg ggcaaggcga
      301 tcttcccctc ggggccgatc cgctcccggg tgtaggtctg cacgccgacg aagtcgtcgt
      361 cgcgggagac ctcgagccag tcgaggctcg ccccctgccg ggcttgctcc cagcgggcct
      421 cgcccccctc agcgggctgg aggtcgacca gcgccagcgt ccaacccacc ttggcgtcac
      481 cggggccggc tttgattgcc tccaccgccc ggcggtggat ggcggccatc tgctcgatgc
      541 ccgcgccgac caggcccatg cggtaccgag cgggatcgaa gccgccgacg ccggccggcg
      601 ccggcgcctc gacctcctgg acggggccgc tctggccgac tcccatcgga atggcgccgg
      661 tctgctgcat caggtcgagg acgttgggct cgttgagcgt ggccacccac ggcaggaggt
      721 cgcctaggtg ctcggtcacc cgctcggcgt agcgtccgaa ccggtcgggg gcgtcggccg
      781 cggcccagcc gcccgccccg gcgaaccagc gcggcgtggt gaagtggcag taggtcacga
      841 ccggcgtgac cccgtgctcc aggcaggtgc cgaccatccg ccggtagtgg tcgagggcag
      901 cgcgggagaa ggcgcccggc tccggctcga tccgggacca ctcgaccgag aaccggtagg
      961 cgttcagtcc cagctcggcc agcgtggcga tgtcctcggc gtaccggtgg tagtggtcac
     1021 aggcgtccag cgacggctcg acgaactgcg agccctcggc ccactcgagg ggccagaggt
     1081 cggcgttcac gttgccgccc tcgacctggt gcccggccgt ggacgatccc cagaggaagt
     1141 cactcgggaa gggggccatg gcgggacact ccgtgtcggt cggtcgggtg gagaaccaag
     1201 ctaggtcagc gacgaccagc tgatctccag gtgggcgcgg agcgagcggc ccggctcgac
     1261 gaccttcggg tcccggttga aggcgtcggg ggcggcggtc tggggctcga cgcagacgag
     1321 ccgggggtcc tggtcataga cgacccacac cgggcaactc gaccggagcg tgacctcgac
     1381 ggccccgggc caggtgatcc tcgggtcagc ccggagcccg ccgaaggcgt cgtcccaggg
     1441 gccggccggg accggcacct gctgaccggt cgggatgccc gatgcgtcgc gctcccacat
     1501 cgacgtcgcc gggacggtca gctgggctgc ctcgcccacg tcgagccggc ggcgaaagca
     1561 cgggtgccag cccatcacga ggggcatcgc ctgctcggcc gcggtcaccg tcatcgtgac
     1621 ggtcaacgag gtcggcgtga gcgcgacgtc ctgggtcacc gttcccccga acggccagtg
     1681 gccgtcgagg tccagccgaa gcgagcccgg gccggtctcc tgccacgggg tgcggtaccc
     1741 gacgccgtgg atggcgtggg gcggggcgtc gagggggagc tcgtgcgtcg tgccccggaa
     1801 ggcgaagtgg ccgtcccgga cccggcccgc ccacgggacc atcgggtagc agccccagag
     1861 cttcgggtcg ccggcctcgg gctcggctat gagcagctcg tggccggcaa cccgcagcga
     1921 ggccacccgg cccccacgag cggggtcgac ggtgaccgcg acgtcacccg ctcgtaggtc
     1981 catggccgcc tccgctcacc gaacttgcgt cagttggtca cgctaggcgt gacccacggg
     2041 cgccagatgg ccgggctcca tgagagaatc ggccgcatga ccatggtcga aggcgagccc
     2101 accgagcgcc gagcggtcaa gagcttctgc cggatctgca ccgcggtgtg cgggatcgtc
     2161 gtcgaggtcg a
//
LOCUS       DASTFV010000555         2707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2064489, whole genome shotgun sequence.
ACCESSION   DASTFV010000555 DASTFV010000000
VERSION     DASTFV010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2064489"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VFP02_10180"
     CDS             complement(<1..259)
                     /locus_tag="VFP02_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543440.1"
                     /translation="MDQPKIVTKAELEAPPEEQTAGMRRGEAFAHDHVWAGHSVFPGG
                     ATTGWHHHGDYATYGYITDGSMTLEYGPDGTHVDHIAAGDFV"
     gene            complement(490..756)
                     /locus_tag="VFP02_10185"
     CDS             complement(490..756)
                     /locus_tag="VFP02_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543441.1"
                     /translation="MDDAGLTDEELAREALAADPDQPLPADAVALRDERDAGAELLPE
                     WYMPRPVHGRVTPARRAVGLALVAAALVINGLGFCLTYGHLTAG"
     gene            800..1183
                     /locus_tag="VFP02_10190"
     CDS             800..1183
                     /locus_tag="VFP02_10190"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9543442.1"
                     /translation="MPELTLVEATTLFARRRDAWLAEDVEAYLACFAADLVITVPGRP
                     EPIRGIEAYERLVRRSFAWARPRSFDFHHVAAGPDATVLAEWSISTERRADRVMAAWR
                     GMSVCAIEEDRIAWWREYWDPAQLA"
     gene            complement(1196..2386)
                     /locus_tag="VFP02_10195"
     CDS             complement(1196..2386)
                     /locus_tag="VFP02_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543443.1"
                     /translation="MTTIPSPAPYAAPVDGARAGAHVSARTGWMAVAGLASLGAGAIH
                     AAAIGVHAEHRPAAVTFAIIAAFQLAWGALALVRSNRVVALIGIGIGVGSFAGWIWAK
                     TWGIAFIPGLDVAEPVQTADGLAAGLAAASVLLIGLALWTGRRGATPSRPPMVAAALG
                     LTALSVFGMIAAGTHVHSDGAGGHSHGTTVVANQEPQVVVPYDPTQPINFSGVPGVTP
                     EQQAAAENIVSATLLLLPQWSDPEYALAHGFQSIGDGFLGTEHFINDTYIDDDKILDP
                     TRPESLVWDTTTGERRLVAAMYMLRRGTPLEDAPNIGGSLMQWHIHNNLCYTPAGQVV
                     GLTNSDGECGPGLVLPEPTPMIHVWLEPQECGPFAALEGIGGGQIAEGETVNCDHVHG
                     STTS"
     gene            2444..>2707
                     /locus_tag="VFP02_10200"
     CDS             2444..>2707
                     /locus_tag="VFP02_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET9543444.1"
                     /translation="MAAGTGTTSALTRERVVVAAIALAERDGLAELSMRRLAAELGAG
                     TMSLYNHVSDKADLFDGMVEAVLAPVRIADSADWREIVATWATD"
BASE COUNT          365 a          905 c         1046 g          391 t
ORIGIN      
        1 acacgaagtc accggcggcg atgtggtcga cgtgggtgcc gtcggggccg tattcgaggg
       61 tcatgctccc gtcggtgatg tagccgtagg tggcgtagtc cccgtggtgg tgccaaccgg
      121 tggtcgcccc gcccggaaag accgaatgcc ccgcccagac gtggtcgtgg gcgaacgcct
      181 ctccccgtcg catccccgcg gtctgctctt cgggtggcgc ctcgagctcg gccttggtca
      241 cgatcttcgg ctgatccact cgggctcctg tccgccgagt cggtcgccac ctgaggggcg
      301 atgtcccgac cgggctgtcc gcgaatgctg acacgattcg ggggtgggtg ctctaggccg
      361 ggcccagcca gaagtcgaag atcagggacg tggcgtcgat gtcggtggtg gtggggccga
      421 actggttccc ggggaggcga ccgggtcatc ggaccacgcg gcggcctcgg gggtacgacg
      481 gcctgcgctt cagccggcgg tcaggtgccc gtaggtcagg cagaagccca ggccgttgat
      541 gaccagggcg gcggcgacta gggccaagcc cacggctcgg cgggccgggg tgacccgacc
      601 gtggacgggc cggggcatgt accactcggg caggagctcg gccccggcgt cccgctcgtc
      661 ccgcagggcc acggcgtcgg cgggcagggg ctggtccggg tcggccgcga gggcctcacg
      721 ggccagttcc tcgtcggtga ggccggcgtc gtccatgacc ctgaccctag gcccggtgcc
      781 gtcccgtagg ttggcgccca tgcccgagct gaccctggtc gaggcgacca cgctgttcgc
      841 ccggcggcgc gacgcctggc tggccgagga cgtcgaggcc tatctggcct gcttcgccgc
      901 cgacctggtc atcaccgtgc ccggccgccc ggagccgatc cgaggcatcg aggcctacga
      961 acggctggtc cggcggtcct tcgcctgggc ccggccccgt tccttcgact tccaccacgt
     1021 cgccgccggc cccgacgcca ccgtcctggc cgaatggtcc atcagcaccg agcgccgggc
     1081 cgaccgggtc atggccgcct ggcggggcat gagcgtgtgc gcgatcgagg aggatcgcat
     1141 cgcctggtgg cgggagtact gggacccggc tcagctggcg tgagccggac gcgggtcagc
     1201 tggttgtcga cccgtgcacg tggtcgcagt tgacggtctc gccttcggcg atctgccccc
     1261 cgccgatgcc ctcgagggcc gcgaacgggc cgcactcctg gggctcgagc cagacgtgga
     1321 tcatgggggt gggctcgggg agcaccaggc cgggaccgca ctcgccatcg ctgttggtga
     1381 ggcccaccac ctggccggcc ggcgtgtaac agaggttgtt gtggatgtgc cactgcatga
     1441 gcgacccgcc gatgttcggc gcgtcctcca ggggcgtgcc ccggcggagc atgtacatgg
     1501 cggccacgag gcggcgctcg ccagtggtcg tgtcccagac cagcgattcc ggtcgggtgg
     1561 ggtcgagaat cttgtcgtcg tcgatgtagg tgtcgttgat gaagtgctcg gtgcccagga
     1621 acccgtcgcc gatcgactgg aagccgtggg ccagcgcgta ctcgggatcc gaccactgcg
     1681 ggagcaggag cagggtggcc gagacgatgt tctccgccgc cgcctgctgc tcaggggtca
     1741 cgccggggac accgctgaag ttgatgggct gggtcgggtc gtaggggacg acgacctgtg
     1801 gttcctggtt ggccaccacg gtggtgccat ggctgtggcc gccggcgccg tcggagtgca
     1861 cgtgggtgcc ggcggcgatc atgccgaaga ccgacagtgc ggtcaggccg agggcggcgg
     1921 cgaccatggg gggccgcgac ggcgtggcgc ccctgcgccc ggtccagagc gccaggccga
     1981 tgagcaggac gctggcggcg gcgaggccgg cggcgaggcc gtcggcggtc tggaccggtt
     2041 cggccacgtc gaggcccggg atgaaggcga tgccccaggt cttggcccag atccaaccgg
     2101 cgaaggagcc gacgccgatc ccgatgccga tcagggccac gacgcggttc gagcggacga
     2161 gggcgagggc gccccaggcc aactggaagg cggcgatgat ggcgaaggtg actgcggccg
     2221 gccggtgctc ggcgtggacg ccgatggcgg cggcgtggat ggcgcccgcg cccagcgagg
     2281 cgagcccggc gacggccatc cagcccgtgc gggcggagac gtgtgcgccg gcccgagcgc
     2341 catccaccgg cgcggcgtag ggagcggggg acgggatcgt cgtcacggcg tacagcgtac
     2401 gtcatcgaac caggatcggt cagcagcacc tacgcttccc gccgtggccg caggaacggg
     2461 aaccacctcg gccctcaccc gagagcgggt ggtcgtcgcc gccatcgccc tcgccgagcg
     2521 ggacggtctg gccgagctca gcatgcggcg cctggccgcc gagctcgggg ccgggacgat
     2581 gtcgctctac aaccacgtga gcgacaaagc ggacctgttc gacggcatgg tcgaggccgt
     2641 gctggccccg gtgcgaatcg ccgacagcgc cgactggcgc gagatcgtgg ccacgtgggc
     2701 caccgac
//
LOCUS       DASTFV010000556         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2064675, whole genome shotgun sequence.
ACCESSION   DASTFV010000556 DASTFV010000000
VERSION     DASTFV010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2064675"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VFP02_10205"
     CDS             complement(<1..652)
                     /locus_tag="VFP02_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543445.1"
                     /translation="MTSVGRGTMVLEDGTGVDFAALRAARREKVLAAMAEGGVELLLL
                     GRQGNARYVAGHRPIWRAVVTAWAPFCALRADGGVHLLHTTWDDGVPEEIPHENLSGL
                     SWSPRTIVGDIAAIPGLAEAKVVATDGLSSGPALLLGHLAPEAELVDGESLMRGVRAV
                     KLPAEVECLRTAVALTEGALTASALEIRAGVAPARLKGAFHEAMGRYGISHPNAEGP"
     gene            complement(649..1809)
                     /locus_tag="VFP02_10210"
     CDS             complement(649..1809)
                     /locus_tag="VFP02_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET9543446.1"
                     /translation="MRAERTTKLRDQMAGRAVDAVVALTTGGVLYATGAHSPAADTGQ
                     TFARRPVAVVLAEDPWPHLFTPYPGSAPDELPADHVHGAFGPETEAGAVAMATTVVDL
                     LGGARGRVAVDDYTMPMWSAFPRVLDGAELVDAGPLFTAARLHKSADEVECLRRSWRM
                     NEVATRTVEAMLRPGVRASELSGAYLSALFQQGATTNFLDPVFQPMPDRIADGPWSTN
                     GDIPFNLVTTDHVLSEGEVIWTDTVSGYEGYASDVGRTWVVGPVSAVRHELHQRWEAI
                     TAAVLAELRPGVTADVLTRVATEVNGGAKPWLDHFFLGHTLGLEGGEMQHIGSDKGQA
                     YDEQLVLEPGMTVVIEPVTWEDGHAGWRCEELVVITEAGHERISAYPEEPVG"
     gene            1875..>2608
                     /locus_tag="VFP02_10215"
     CDS             1875..>2608
                     /locus_tag="VFP02_10215"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9543447.1"
                     /translation="MEHERSESAAEIRAALPHPVVDGDGHLIEYLPALAGYLREEGID
                     PAGPTVQRLLPGRFGPDVDWYGASPEDRARLRIARPPWWGAPARNTRDLATALFPSLL
                     HERLDELGIDVSVVYPSMGLAFLHLEDVDERTGACRALNRYCQEQFDAHRDRLVPVAA
                     IPMHTPEEAVAALDHAVGTLGFKAVLLAGYVQRSVEGVSADAAPWARWIDTYGLDSAH
                     DYDPVWARCRDLKVAVAFHSGSIGWG"
BASE COUNT          347 a         1013 c          892 g          356 t
ORIGIN      
        1 acgggccctc ggcgttgggg tggctgatgc cgtagcggcc catcgcctcg tggaacgcac
       61 ccttcagccg ggccggggcg acacccgccc ggatctcgag ggcggacgcc gtcagagccc
      121 cctcggtcag ggccacggcc gtgcggaggc actcgacctc ggccgggagc ttcacggccc
      181 ggaccccccg catcagggac tcgccgtcga ccagctcggc ctcgggggcc aggtgcccca
      241 ggagcagggc cgggcccgac gacaggccgt cggtggccac gaccttcgcc tcggccagcc
      301 cggggatggc ggcgatgtcg cccacgatcg tgcgcgggct ccaggacagg ccgctcaggt
      361 tctcgtgcgg gatctcctcg ggcacgccgt cgtcccacgt cgtgtgcagc agatggaccc
      421 cgccatcggc ccgcagagcg cagaacggcg cccacgccgt gaccaccgcc ctccagatgg
      481 gtcgatgccc ggccacgtag cgggcgttgc cctgccggcc caacagcagt agctcgacgc
      541 caccctccgc catggcggcc agcaccttct cgcgccgggc cgcccgcagc gcggcgaagt
      601 cgacgccggt gccgtcctcc aacaccatcg tcccccgacc caccgacgtc atcccacggg
      661 ctcctcgggg taggcgctga tgcgctcgtg gccggcctcg gtgatcacga ccagctcctc
      721 gcagcgccaa ccggcgtggc cgtcctccca ggtcacgggc tcgatcacga cggtcatgcc
      781 cggctccagc accagctgct cgtcgtaggc ctggcccttg tccgacccga tgtgctgcat
      841 ctcccctccc tccaggccca gcgtgtggcc gaggaagaag tggtccaacc acggcttcgc
      901 gccaccgttg acctccgtgg ccacgcgggt gagcacgtcg gcggtgacgc cgggtcgcag
      961 ctcggcgagg acggcggcgg tgatggcctc ccaccgctgg tgcagctcgt gtcggactgc
     1021 gctcactggt ccgaccaccc aggtccggcc gacgtcggag gcgtagccct cgtagcccga
     1081 cacggtgtcg gtccagatca cctcgccctc ggagagcacg tggtcggtcg tgaccaggtt
     1141 gaacgggatg tcgccgttcg tgctccaggg cccgtcggcg atccggtcgg gcatgggctg
     1201 gaagaccgga tccagaaagt tggtcgtcgc cccctgctgg aacagggccg acaggtaggc
     1261 cccactcagc tcggacgccc gcacgccggg ccggagcatc gcttcgaccg tcctggtggc
     1321 gacctcgttc atccgccacg accggcgcag gcactcgacc tcgtcggcgg acttgtgcag
     1381 gcgggccgcg gtgaagagcg ggccggcgtc gaccagctcg gcgccgtcca ggacccgcgg
     1441 gaaggccgac cacatcggca tcgtgtagtc gtcgaccgcc acccggcccc tcgccccgcc
     1501 gagcaggtcg acgaccgtgg tggccatcgc caccgcgccc gcctccgtct cgggcccgaa
     1561 ggccccgtgg acgtggtcgg cgggcagctc gtccggcgcg ctccccggat agggcgtgaa
     1621 caggtggggc cacgggtcct cggccagcac gacggcgacc gggcgtcggg caaaggtctg
     1681 gcccgtgtcg gcggccggtg agtgggcccc cgtggcgtag agcaccccac ccgtggtgag
     1741 ggcgacgacg gcgtcgactg cccgcccggc catctggtcc cgcagcttgg tcgtccgctc
     1801 ggcgcgcatc cggacgaggt cgagcgggaa cggcgctggc tggcccgaca taatgcggtt
     1861 acattagcac cccgatggag cacgagaggt ccgagtccgc ggccgagatc cgagccgcgc
     1921 tgccccaccc cgtggtcgac ggcgacggcc atctcatcga gtacctgcct gccctggccg
     1981 gctacctccg cgaggagggc atcgatcccg ccggccccac cgttcagcgc ctgctccccg
     2041 gccgcttcgg ccccgatgtg gactggtacg gggccagccc cgaggaccgg gcccgcctcc
     2101 gcatcgcccg gccaccgtgg tggggcgccc ccgcccgcaa cacgcgcgac ctggccaccg
     2161 ccctcttccc cagcctcctc cacgagcgcc tcgatgagct cgggatcgac gtcagcgtcg
     2221 tctacccgag catgggcttg gccttcctcc acctcgagga cgtcgacgag cgcaccggcg
     2281 cctgccgggc cctcaaccgg tactgccagg agcagttcga cgcccaccgc gaccgcctgg
     2341 tccccgtggc cgccatcccc atgcacaccc ccgaggaggc cgtggccgcc ctcgatcacg
     2401 ccgtggggac cctcggtttc aaggccgtgc tcctcgccgg gtacgtgcag cgatcggtcg
     2461 agggtgtgtc cgccgacgcc gccccgtggg cccgctggat cgacacctac ggcctggaca
     2521 gcgcccacga ctacgacccc gtgtgggccc gctgccggga cctgaaggtg gcggtggcct
     2581 tccactccgg ctcgatcggg tggggcag
//
LOCUS       DASTFV010000557         2581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2064861, whole genome shotgun sequence.
ACCESSION   DASTFV010000557 DASTFV010000000
VERSION     DASTFV010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2064861"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VFP02_10220"
     CDS             <1..637
                     /locus_tag="VFP02_10220"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HET9543448.1"
                     /translation="ANSPYWNGEDTGYDSYRLEVWERWPTAGLPPALADRAAYDEVVD
                     ELQEADAIDDATHLYWYLRPSDRYPTLEFRATDVCLDVDAAVCLAGLARALVRTCVAD
                     AQAGVPIGTASSSLLGASLWRAARYGLAGDLVSPSSAAPLPAADVVNELLTRVGGALD
                     QAGDREEVTALAEAILRIGNGARRQREALAQRGDPADVVALGRVGLLAPAD"
     gene            718..2142
                     /locus_tag="VFP02_10225"
     CDS             718..2142
                     /locus_tag="VFP02_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9543449.1"
                     /translation="MRDHDKIYIDGAWVPSTGSGSIEVVNASTEQVMGSIPEGTAEDV
                     DKAVAAAKAAFETWGRTEPEERGKFIQRLAEGLGARSEEIAQVISEEMGMPLMWANMI
                     QAGLPVGNMGTYVEILSDFPWEERIGNSLVVREPIGVVGCITPWNYPLHQIVNKVAPA
                     LAAGCTVVLKPSEIAPLDAFILAEVIDEVGLPAGVFNLVTGFGPVVGEAIAAHPDVDM
                     VSFTGSTRAGKRVSVLASDTVKRVALELGGKSANIILDDADLAEVVPKGVFACYLNSG
                     QTCTAHTRMLVPKEKHDEAVAIAAAAAEGFPVGPADQDGVMLGPLSSAAQRDRVQDYI
                     RKGVDEGATLATGGPEAPEGLDTGYFVRPTVFGNVSSDMTIAQEEIFGPVLSIIPYED
                     EEDAIRIANDTPYGLAGGVWSADEERAQRVARRMRTGQVDVNGGSFNIKAPFGGYKQS
                     GVGRELGRYGLEEFLEVKSMQLND"
     gene            2215..>2581
                     /locus_tag="VFP02_10230"
     CDS             2215..>2581
                     /locus_tag="VFP02_10230"
                     /inference="COORDINATES: protein motif:HMM:NF017073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2-like N-terminal transpeptidase
                     domain-containing protein"
                     /protein_id="HET9543450.1"
                     /translation="MLLLVPSCSVLGREGADGASAEETAAVYLNAWAAGDAAAMAAVV
                     IDPPADFASVQQQFRASLHVATAEVADIEVESSGDEATAAFAADLGLQGLGHWAYDGT
                     VDLQRVDGTWGVAFTPASLH"
BASE COUNT          386 a          916 c          928 g          351 t
ORIGIN      
        1 cgccaactcc ccgtactgga acggtgagga caccggctac gactcctacc gcctcgaggt
       61 ctgggaacgg tggccgacgg ccggcctacc cccggccctg gctgatcggg cggcctacga
      121 cgaggtcgtg gacgagctcc aggaggccga cgccatcgac gatgccaccc acctgtactg
      181 gtacctgcga ccgtccgatc gctatccgac cctcgagttc cgggccaccg acgtgtgcct
      241 ggacgtcgac gcggccgtgt gcctggccgg cctggccagg gcgttggtca ggacctgcgt
      301 ggccgatgcc caggccggag tgccgatcgg gaccgcctcg tcctcgttgc tcggcgcgtc
      361 tctgtggcgg gccgcccgct acggcttggc gggcgacctc gtgtcgccga gctcggccgc
      421 gccgcttccc gccgcggacg tggtgaacga gctgctgacc agggtgggcg gtgctctcga
      481 ccaggcgggc gaccgtgagg aggtcacggc gctggccgag gcgatcctgc ggatcgggaa
      541 cggggcccgg cgccaacgag aggcgttggc gcagcgcggc gatccagctg acgtcgtcgc
      601 cctcgggcgg gtcggcctcc tggccccggc cgactgacgc gatcaacggt cgggcgagta
      661 ccgtcgcgcg tccaccagaa gcgcagccgg accacacacg aaaccacagg gggactcgtg
      721 cgcgaccacg acaagatcta catcgacggg gcctgggtgc cctccaccgg gagcgggtcc
      781 atcgaggtgg tgaacgcctc gaccgagcag gtcatgggca gcatccccga gggcaccgcc
      841 gaggacgtcg acaaggccgt cgccgcggcg aaggcggcgt tcgagacctg gggccggacc
      901 gagcccgagg agcggggcaa gttcatccag cgcctggccg agggcctcgg cgcccggtcc
      961 gaggagatcg cccaggtcat ctccgaggag atgggcatgc ccctcatgtg ggccaacatg
     1021 atccaggccg gcctgccggt cgggaacatg gggacctacg tcgagatcct ctcggacttc
     1081 ccctgggagg agcgcatcgg caactcgctg gtcgtgcggg agccgatcgg cgtggtcggc
     1141 tgcatcacac cctggaacta ccccctccac cagatcgtca acaaggtcgc cccggccctg
     1201 gccgcgggtt gcacggtcgt cctgaagccc agcgagatcg ccccgctgga cgccttcatc
     1261 ctggccgagg tcatcgacga ggtcggcctg cccgccgggg tgttcaacct cgtcaccggc
     1321 ttcggcccgg tggtggggga ggccatcgcc gcccaccccg atgtcgacat ggtcagcttc
     1381 accggctcca cccgggccgg caagcgggtc agcgtcctcg ccagcgacac ggtcaagcgg
     1441 gtcgccctcg agctcggggg caagtcggcc aacatcatcc tggacgacgc cgacctcgcc
     1501 gaggtcgtgc ccaagggcgt gttcgcctgc tacctgaact cgggccagac ctgcacggcc
     1561 cacacgcgga tgctcgtgcc gaaggagaag cacgacgagg ccgtcgccat cgccgcggcc
     1621 gcggcggagg gcttccccgt cggaccggcc gaccaggacg gggtcatgct cggccccctc
     1681 tcgtcggccg cccagcgcga ccgggtccag gactacatcc gcaagggcgt cgacgagggg
     1741 gccacgctgg ccaccggcgg ccccgaggcg cccgagggcc tggacaccgg gtacttcgtc
     1801 cgcccgacgg tgttcggcaa cgtgtccagc gacatgacca tcgcccagga ggagatcttc
     1861 ggccccgtgc tgtcgatcat cccctacgag gacgaagagg atgcgatccg catcgccaac
     1921 gacaccccgt acggcctcgc cggtggggtg tggtcggccg acgaggagcg ggcccagcgg
     1981 gtcgcccgtc ggatgcgcac cggccaggtc gacgtcaacg gcggcagctt caacatcaag
     2041 gcgcccttcg gtggctacaa gcagtccggg gtcggtcgtg agctcgggcg gtacggcctg
     2101 gaggagttcc tcgaggtgaa gtccatgcag ctgaacgact gagcccgagc ggatcaggag
     2161 cgcaggctgg ggtggcgaga gggaaaggga cggatcgcgg cgaccacggt cgccgtgctg
     2221 ctcctcgttc cctcgtgctc ggtcctcggc cgcgagggcg ccgacggcgc ctcggctgag
     2281 gagacggccg ccgtctacct caacgcgtgg gcggcgggtg acgccgcggc catggccgca
     2341 gtggtcatcg acccgccggc ggacttcgcg tccgtccagc agcagttccg ggccagcctg
     2401 cacgtggcca cggccgaggt ggccgacatc gaggtcgaat cgtcggggga cgaggccacg
     2461 gccgccttcg ccgccgacct cggcctccag ggcctcgggc actgggccta tgacggcacc
     2521 gtcgacctgc agcgggtgga cgggacctgg ggcgtggcgt tcacgccggc atccctccac
     2581 c
//
LOCUS       DASTFV010000558         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2066032, whole genome shotgun sequence.
ACCESSION   DASTFV010000558 DASTFV010000000
VERSION     DASTFV010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066032"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFP02_10235"
     CDS             complement(<1..316)
                     /locus_tag="VFP02_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543451.1"
                     /translation="MAQRGLLKRRRTAPVVALCLGAALLVSCSSDDSGPPHLVWYINP
                     DNGGQATLAQQCTEQSDGAYSIETQVLPNEADAQREQLVRRLAAGDSSVDLMSLDPPF
                     VAE"
     gene            complement(363..1973)
                     /locus_tag="VFP02_10240"
     CDS             complement(363..1973)
                     /locus_tag="VFP02_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HET9543452.1"
                     /translation="MAPDPSPPNEQDGVPWWREAVIYEIYVRSFADGDGDGLGDLAGI
                     RSRLPALAELGVDAIWLTPFYQSPQADGGYDVADPCAVDPRFGTMADFDALLTTTHEL
                     GLRLLVDIVPNHTSVDHPWFRAALAGGPGSPARDRYWFRPGRGPAGEEPPNDWRSVFG
                     GPAWTRITEPDGEPGPWYLHLFAPEQPDLDWTRAEVRKGFDSILAFWLDRGVDGFRID
                     VAHGLAKDPELADIAGRFAHAGVAAEGHPHWDRDEVHEIYRGWRHLIDTYPGEQVFVA
                     EAYLGSARRLARYVRPDELHTAFNFDLLLAPWDATALTEAIDESITGLAEVGATPTWV
                     LSNHDMVRHVTRYGGGDLGLRRARAAIQLLLALPGAAYLYQGEELGLPEVTDLPDDAL
                     QDPTFVRSGGEERGRDGCRVPLPWAEDRPSYGFGPGPKAWLPQPADWAALTRERQAAD
                     PDSMLELYRSALALRRGLPSLGVGPFAWMEGPPGVIAFHRGPDFTCVVNVGDDAVALD
                     ATGRIVLASGPLGSTGSLPGVTGVWLTS"
BASE COUNT          291 a          702 c          764 g          273 t
ORIGIN      
        1 actcggcgac gaagggcggg tcgaggctca tgaggtcgac cgacgagtca cccgcggcga
       61 ggcggcgcac cagctgctcc cgttgggcgt cggcttcgtt gggcaggacc tgggtctcga
      121 tggagtaggc cccgtcggac tgctcggtgc actgctgggc cagcgtggcc tggccgccgt
      181 tgtccgggtt gatgtaccag accaggtgcg gcgggccgga gtcgtcgctc gagcaggaga
      241 cgaggagcgc ggctcccagg cagagagcga cgaccggtgc tgtccgacgc cgcttcagca
      301 acccccgctg agccatccgt ccccctctgt gctgaccatc aaaggcgggc ttgtcccaga
      361 agtcaactgg tcagccagac ccctgttacc cccggcagcg atccggtaga cccgaggggg
      421 ccactggcca gcacgatccg gccggtcgcg tcgagggcca cggcgtcgtc gccgacgttg
      481 accacacagg tgaagtccgg cccccggtgg aaggcgatga cccctggtgg gccctccatc
      541 caggcgaagg ggccgacgcc gagcgacggc agaccccggc gcagggccag agccgagcgg
      601 tacagctcca gcatggagtc ggggtcggcg gcctgccgct cccgggtcaa cgccgcccag
      661 tcggccggtt gggggagcca cgccttcgga cccggtccga acccgtagga gggccggtcc
      721 tcggcccacg gcaacggcac ccgacacccg tcccgtcccc gttcctcgcc gcccgagcgg
      781 acgaacgtgg ggtcctgcag ggcgtcgtcg ggcaggtcgg tcacctcggg caggccgagc
      841 tcctcgccct ggtagaggta ggccgcgccg ggcagggcga gcaagagctg gatggcggcc
      901 cgggcccggc gcaggccgag gtcaccgccg ccgtagcggg tcacgtggcg caccatgtcg
      961 tggttcgaca gcacccaggt cggcgtcgct ccgacttcgg ccaggcccgt gatgctctcg
     1021 tcgatggcct cggtcagggc ggtggcgtcc cacggggcca acaggaggtc gaagttgaag
     1081 gcggtgtgca gctcgtcggg ccggacgtag cgggccaagc ggcgcgcact gccgaggtac
     1141 gcctccgcca cgaagacctg ctcgcccggg taggtgtcga tgaggtggcg ccagccccga
     1201 tagatctcgt ggacctcgtc ccggtcccag tgggggtggc cctcggcggc cactccggca
     1261 tgggcgaagc gtccggcgat gtcggccagc tcggggtcct tggccagccc gtgggccacg
     1321 tcgatgcgga acccgtcgac accccggtcg agccagaagg ccaggatcga gtcgaacccc
     1381 ttccgcacct ccgctcgggt ccagtcgagg tcgggttgct cgggggcgaa caggtggagg
     1441 taccacgggc cgggctcacc gtcgggctcg gtgatgcggg tccacgccgg gccgccgaag
     1501 acgctgcgcc agtcgttggg gggctcctca cccgccggac cgcgaccggg tcggaaccag
     1561 tagcggtccc gcgcggggct cccggggcca ccggccagag cggcgcggaa ccagggatgg
     1621 tccaccgagg tgtggttggg cacgatgtcg accagcaggc gcaggccgag ctcgtgggtg
     1681 gtggtcagga gggcgtcgaa gtcggccatc gtgccgaacc gtgggtcaac ggcgcacggg
     1741 tcggcgacgt cgtagccccc gtccgcctgc ggcgattggt agaagggcgt cagccagatg
     1801 gcgtccaccc cgagctccgc caaggccggg agccgggacc ggatgccggc caggtcaccc
     1861 agcccgtcgc cgtcgccgtc ggcgaagctg cggacgtaga tctcgtagat gaccgcctcc
     1921 cgccaccatg ggaccccgtc ctgctcattc ggtgggctcg ggtcgggggc cattcccgcg
     1981 atcctcgcgg accggtcaag gtcgggcgac cgcacggggg cggtcgatca
//
LOCUS       DASTFV010000559         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2068935, whole genome shotgun sequence.
ACCESSION   DASTFV010000559 DASTFV010000000
VERSION     DASTFV010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068935"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VFP02_10245"
     CDS             complement(<1..488)
                     /locus_tag="VFP02_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET9543453.1"
                     /translation="MSSPTHEAVLAAAHEMLDKGLVAGTAGNVSGRMPDGNVVMTPSS
                     VAYETMTMDDLVVVDLDGKVVDGERGPTSEKALHLSCYRSYDEVGGVIHCHATYASMF
                     ALVRQPIPAVIEEFVVYVGGDVPIAAYKPTGSDELADEIATHLTDRSCALMANHGLLA
                     IGR"
     gene            complement(559..1533)
                     /locus_tag="VFP02_10250"
     CDS             complement(559..1533)
                     /locus_tag="VFP02_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9543454.1"
                     /translation="MSRPKALVTAPFRGAGMATLEGVADVVYDPWIEHTPLKLMNDEA
                     LAVRIAEEGADIVVCEADACTGAVLDAGLRVIGSTRGDPTNVDVPGATERGIPVLHAP
                     GRNADGVAELAVGLLLAATRGIVPADKDVRTGTVYDGGTIPYQRYRAWQVAGQTVGII
                     GLGSVGRAAAWRFEGLGMKVIAHDPFNDAATYELDDLLAEADVVSMHATVTPDTLGLL
                     GTHQFARMKPGAVYLNTARAALHDIDALTAALASGHLGAAGIDHFEGENLPTDHPLQT
                     MTNVVLTPHIGGATYDTEANHSTLVADDIARLLAGERPVHCANPEVLQ"
     gene            1601..>2789
                     /locus_tag="VFP02_10255"
     CDS             1601..>2789
                     /locus_tag="VFP02_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9543455.1"
                     /translation="MDLPKIISVDDHVVEPPHVWQEWLPEKWRERGPKVERKRWGKFR
                     HKAGARYDMTEDPEGRWGDAWYYDGRLIYVHKNFVAIPQAATTVNAQGTIDFDRSHME
                     MTPATYDDMRPGCWQVEERIKDFELNWVDGSLPFPTFPRFCGQTFYEGEDKELGLACV
                     RAYNDWMVEEWCGTSRGYNIPLCLIPLWDVELAAAETQRNADRGVRAICFSELPHHLS
                     LPTIHSGYWDPLWQVCNDNGVTVCMHIGSSSTTPLASPDAPKAVGGMIAFNNCMASMG
                     DWLFSRKLIEFPELKLAFSEGQIGWIPYALERADTVWDHHDSWMNSKARIPEPPSTYY
                     YGRIFGCFTADRHGLANLEAVGPDNICFETDYPHTDTTWPDSKAYAEKMVAGLDDEAV
                     YKIL"
BASE COUNT          417 a          941 c          991 g          440 t
ORIGIN      
        1 cggccgatgg ccagcaggcc gtggttggcc atgagggcgc aggagcggtc ggtcaggtgg
       61 gtggcgatct cgtcggccag ctcgtcgctg ccggtcggct tgtaggccgc gatgggcacg
      121 tcaccgccca cgtagacgac gaactcctcg atgaccgccg ggatgggctg gcggaccagg
      181 gcgaacatgg aggcgtaggt ggcgtggcag tggatgaccc caccgacctc gtcgtagctg
      241 cggtaacagg acaggtgcag ggccttttcg ctggtcgggc cccgctcgcc gtcgaccacc
      301 ttgccgtcga ggtcgacgac caccaggtcg tccatggtca tggtctcgta ggccaccgac
      361 gacggggtca tgacgacgtt gccgtcgggc atgcggcccg acacgttgcc cgccgtgccc
      421 gccaccaggc ccttgtcgag catctcgtgg gcggccgcga gcacggcctc gtgggtgggc
      481 gagctcatcg ttgtcggctc ttcgcctgac ggctcatcgc ggtgcaggcc tgttcggtcc
      541 gccgatcgac ttcgtcgctc attgcagcac ctccgggttg gcgcagtgga cggggcgctc
      601 gccggcgagc aggcgggcga tgtcgtcggc caccagcgtc gagtggttgg cttcggtgtc
      661 gtaggtggcg cccccgatgt gaggcgtcag gacgacgttg gtcatggtct ggagcgggtg
      721 gtcggtcggc aggttctcgc cctcgaagtg gtcgatcccg gcggcgccga ggtggcccga
      781 cgccagggcc gcggtcaggg cgtcgatgtc gtggagggcg gcccgagcgg tgttcaggta
      841 gaccgcgccc ggcttcatgc gggcgaactg gtgggtgccg agcaggccga gcgtgtcggg
      901 cgtgaccgtg gcgtgcatcg agacgacgtc ggcctcggcc agcaggtcgt cgagctcgta
      961 ggtggcggcg tcgttgaagg ggtcgtgggc gatgaccttc atgcccagcc cctcgaaccg
     1021 ccaggccgcg gcccgcccga ccgagccgag gccgatgatg cccacggtct gccccgcgac
     1081 ctgccaggcc cggtagcgct ggtaggggat cgtgccgccg tcgtagaccg tgccggtgcg
     1141 cacgtccttg tcggcgggga cgatcccgcg ggtcgccgcc agcaggaggc cgacggccag
     1201 ctcggccaca ccgtcggcgt tgcggccggg ggcgtggagg accgggatgc cccgctcggt
     1261 ggcacctggg acgtcgacgt tggtgggatc gccccgggtc gagccgatga cccgcaggcc
     1321 ggcgtcgagg acggcgcccg tgcaggcgtc ggcctcgcac acgacgatgt cggccccttc
     1381 ctcggcgatg cggacggcca gcgcctcgtc gttcatcagc ttgagtgggg tgtgctcgat
     1441 ccacgggtcg tagaccacgt cggccacgcc ctcgagggtg gccatgccgg caccccggaa
     1501 cggggcggtc accagtgcct tcggacggct cacggccgcg aaccctacgc cctggctcgt
     1561 acctgacacg cgtgtcaggt tggctgccta cgatgtcgcc gtggacctcc cgaagatcat
     1621 cagcgtggac gaccacgtcg tcgaaccgcc ccacgtgtgg caggaatggc tgcccgagaa
     1681 gtggcgtgag cggggaccga aggtcgagcg caagcgctgg ggcaagttcc ggcacaaggc
     1741 cggggcccgc tacgacatga ccgaggaccc cgaaggtcga tggggcgacg cctggtacta
     1801 cgacggccgg ctcatctacg tgcacaagaa cttcgtggcc atcccgcagg cggccacgac
     1861 ggtcaacgcc cagggcacca tcgacttcga ccgctcccac atggagatga cccccgccac
     1921 ctacgacgac atgcgtcccg gctgctggca ggtcgaggag cgcatcaagg acttcgagct
     1981 caactgggtc gacgggtcgt tgcccttccc gaccttcccc cgcttctgcg gccagacctt
     2041 ctacgagggc gaggacaagg agctcgggct ggcctgtgtg cgggcgtaca acgactggat
     2101 ggtcgaggag tggtgcggga cctcgcgggg ctacaacatc ccgctctgcc tgatccccct
     2161 ctgggacgtc gagctggccg cggcggagac ccagcgcaac gccgaccggg gcgtgcgggc
     2221 catctgcttc agcgagctgc cccaccacct gagcctgccc accatccatt cgggctactg
     2281 ggacccgctg tggcaggtct gcaacgacaa cggcgtgacc gtctgcatgc acatcggctc
     2341 gtcgtcgacc acgccactgg cgtcgccgga cgcgccgaag gccgtcggcg ggatgatcgc
     2401 cttcaacaac tgcatggcgt ccatgggcga ctggctcttc tcccgcaagc tgatcgagtt
     2461 ccccgagctg aagctggctt tctccgaggg tcagatcggc tggatcccct acgccctcga
     2521 gcgggcggac acggtctggg accaccacga cagctggatg aactccaagg cccggatccc
     2581 cgagccgccg tcgacctact actacggccg catcttcggc tgcttcaccg cggaccgcca
     2641 cggcttggcc aatctcgagg ccgtcggccc cgacaacatc tgcttcgaga ccgactatcc
     2701 gcacaccgac acgacgtggc ccgactccaa ggcctacgcc gagaagatgg tggcggggtt
     2761 ggacgacgag gcggtctaca agatcttgc
//
LOCUS       DASTFV010000560         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2069321, whole genome shotgun sequence.
ACCESSION   DASTFV010000560 DASTFV010000000
VERSION     DASTFV010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2069321"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..374
                     /gene="rplQ"
                     /locus_tag="VFP02_10260"
     CDS             24..374
                     /gene="rplQ"
                     /locus_tag="VFP02_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET9543456.1"
                     /translation="MPTPRKSRRFGGSASHQKAMMANLAASLFAAEAIVTTEAKAKAL
                     RPIAEKLVTRAREDSVHNRRKVVAFLRDDEMTAKLFEDIGPRYEERPGGYTRILKLGP
                     RHGDNAPMARIELV"
     gene            complement(375..1145)
                     /gene="ppk2"
                     /locus_tag="VFP02_10265"
     CDS             complement(375..1145)
                     /gene="ppk2"
                     /locus_tag="VFP02_10265"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HET9543457.1"
                     /translation="MNKKAYAKELKRLEVEMVALQEWVKATGAKVCVVFEGRDAAGKG
                     GTISALTRRVSARVFRVVALPSPTEREKTQMYVQRYLPHLPAAGEVVIFDRSWYNRAG
                     VEPVLGFCTPEETDHFLEMVPRVERAIVDSGVLLLKYWLEVGEEEQVKRINKRITDPR
                     KTWKLGTLDLKNVALWWDYAKARDEMLRRTSSEWAPWHVVHTDDKEQGRLNLITHLLS
                     QIPYEAPEQRRITLPARNEKPEGYKELDLTPYEVPTPF"
     gene            complement(1145..>2182)
                     /locus_tag="VFP02_10270"
     CDS             complement(1145..>2182)
                     /locus_tag="VFP02_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET9543458.1"
                     /translation="IGDQLHLGEGAMSGPEFAALTDEELEERLPALHVFGRVAPEDKL
                     RLVQIMQRQGLVVAMTGDAVNDAAALKQADIGVAMGTGSEVSKQAAKMVLTDDNFATL
                     VHAVALGRDIWHKITAYIRYQMSQLFGLVSMFLVAALVDLNSGVALLPMQIIALNFFV
                     AVFPVVAIMTDAADPKVMEERPRDPTIPIFNRTTGPRWVAYGLVLGLVSLVPLAWGPD
                     EPHIDSGSVAMTMTFAVIGASTLLSGFAMRYDRRVGLSQPLLRYAATLGGGGLILLLM
                     TELPTLQRWLDTYPLNGGQWVAVLALALVMPVVVELDKAFQRWRERPQEPKPPLDITD
                     ALGGPPLTPAV"
BASE COUNT          341 a          746 c          749 g          346 t
ORIGIN      
        1 atcatccccg cgccggagta gccatgccca cgccccgcaa gtcccgccgc ttcggcggca
       61 gcgcctcgca ccagaaggcg atgatggcca acctggccgc gtcgctgttc gccgccgagg
      121 ccatcgtcac gaccgaggcc aaggccaagg ccctgcgccc catcgccgag aagctggtca
      181 cccgggcccg cgaggacagc gtccacaacc gccgcaaggt ggtggccttc ctccgcgacg
      241 acgagatgac cgccaagctc ttcgaggaca tcggcccccg ctacgaggag cgcccgggtg
      301 gctacacccg catcctcaag ctgggccccc gccatgggga caatgccccc atggcccgaa
      361 tcgaactggt ctgatcagaa cggcgtcggg acctcgtagg gcgtgaggtc aagctccttg
      421 tagccctcgg gtttctcgtt gcgggcgggc agcgtgatcc tccgctgctc cggtgcctca
      481 tacgggatct gcgagagcag gtgggtgatc aggttcagcc gaccctgctc cttgtcgtcg
      541 gtgtggacga cgtgccaggg tgcccactcg ctgctcgtgc gccggagcat ctcgtcgcgg
      601 gccttggcgt agtcccacca cagggcgacg ttcttcaggt cgagggtgcc cagcttccag
      661 gtcttgcggg ggtcggtgat ccgcttgttg atgcgcttca cctgttcctc ctcaccgacc
      721 tccagccagt acttgaggag caggaccccg gagtcgacga tggcccgctc gaccctgggg
      781 accatctcga ggaagtggtc ggtctcctcc ggggtgcaga agccgaggac gggctcgacg
      841 ccggcgcggt tgtaccaact gcggtcgaag atcacgacct cacccgccgc cggcaggtgg
      901 gggaggtacc gctggacgta catctgggtc ttctcgcgct cggtcgggga cgggagggcg
      961 acgacccgga agacacgggc gctgacccgc cgcgtgagag cgctgatcgt gccgcccttg
     1021 cccgccgcgt cccggccctc gaagaccacg cagaccttgg ccccggtggc cttcacccac
     1081 tcctgtaggg cgaccatttc gacctccagc cgcttcagct ccttggcgta cgccttcttg
     1141 ttcatcagac ggccggggtc aggggaggac cgcccagggc atcggtgatg tcgagtggcg
     1201 gcttcggctc ctgcggacgc tcgcgccatc gttggaaggc cttgtccagc tccacgacga
     1261 cgggcatgac cagcgcaagg gcgagcacgg cgacccactg gccgccgttc agcgggtagg
     1321 tgtcgagcca gcgctggagc gtgggcagct cggtcatgag cagcaggatc agcccacccc
     1381 cgccgagggt ggcggcgtag cggagcaggg gctggctgag cccgacccgg cggtcgtagc
     1441 gcatggcgaa cccggacagc agcgtgctgg ccccgatgac cgcgaaggtc atcgtcatgg
     1501 ccaccgagcc gctgtcgatg tgcggctcgt ccggccccca ggccagcgga acgagggaca
     1561 ccagtccgag caccaagccg tacgccaccc agcggggtcc cgtcgtgcgg ttgaagatcg
     1621 ggatcgtcgg gtcccggggg cgttcctcca tcaccttggg gtcggcggcg tcggtcatga
     1681 tcgccaccac cgggaacacg gcgacgaaga agttcagggc gatgatctgc atcggcagca
     1741 gcgccacgcc ggagttcaag tccaccagcg cggcgaccag gaacatcgac accaggccga
     1801 agagctggct catctgatac cggatgtagg ccgtgatctt gtgccagatg tcgcggccga
     1861 gggccacggc gtgcacgagg gtggcgaagt tgtcgtcggt cagaaccatc ttggccgcct
     1921 gtttcgacac ctcgctgccc gttcccatcg ccaccccgat gtcggcctgc ttcagggcgg
     1981 cggcgtcgtt cacggcatcg ccggtcatgg ccacgacgag gccttggcgc tgcatgatct
     2041 ggacgaggcg gagcttgtcc tcgggggcga cgcggccgaa gacgtgcagc gcggggagcc
     2101 gctcctccag ctcttcgtcg gtgagggcgg cgaactcggg cccggacatg gcgccctcac
     2161 cgaggtggag ctggtcgccg at
//
LOCUS       DASTFV010000561         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2072255, whole genome shotgun sequence.
ACCESSION   DASTFV010000561 DASTFV010000000
VERSION     DASTFV010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2072255"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1309)
                     /locus_tag="VFP02_10275"
     CDS             complement(17..1309)
                     /locus_tag="VFP02_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET9543459.1"
                     /translation="MSHDELLARHRAVLPSWLALYYDEPIELVEGQGRHVIDGEGNRY
                     LDFFGGILTTMTGYDVPEVTAAIREQAGRMIHTSTLYLIRQQVELAEMIADLSGIPDP
                     RVFFTTSGTEANEAALLVASLFRRSSQIVAVRNSYHGRSFATVGITGNRGWSPSSLTP
                     FQVSYMQSGYRFRSPFRHLDDGDFIEACTEDLRDVIATQTAGDIAALIAEPVQGVGGF
                     AIPPDGFFGPVEKVCREHDMLFIADEVQTGWGRTGDHFWGHQAHGITPDLMTFAKGLG
                     NGLTIAGVVGRAEVMNSVSANSISTFGGNPLSCAGALANLHYLLDHGLQANTKARGAE
                     LMAALAPLPERYPAIGEIRGKGLMIGLEFVGDDGLTPDLQVASAVLEGCRARGLLVGK
                     GGLHGNCLRIAPPLSLTAEEAAEGAAILTTTLDTVLGV"
     gene            1408..2595
                     /locus_tag="VFP02_10280"
     CDS             1408..2595
                     /locus_tag="VFP02_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9543460.1"
                     /translation="MAEIPMIISVDDHVVEPAHLWQTWLPEKHRADAPYVERHGLGGL
                     KYVGGTTYEYELTDDGPPCDIWFYEGKMYPHKRHVASVGFERDDMTLSPITYDEMRPG
                     CYEPKARVDDMLMNHVEASLSFPTFPRFCGQTFAEARDKDLAMACVRAYNDWMVDEWC
                     GDSDGALIPLCLIPLWDIEAAAAEIHRNAARGVRAVCFSEIPSKLGLPSIHTGQWDPF
                     FLACQETQTVVCMHIGSSSQMPATSPDAPPAVAATLSFNNAMASLSDFLFSGVLVRFP
                     TLTLAYSEGQIGWLPYMLERADDVWREHRAWGGVADLVPEPPSTYYYRQVYGCFFRDR
                     HGLDALDKVGVDNITFETDYPHTDSTWPNTLAVAEDLMGHLPQDAVDKICRGNAQRML
                     GLA"
     gene            2645..3430
                     /locus_tag="VFP02_10285"
     CDS             2645..3430
                     /locus_tag="VFP02_10285"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET9543461.1"
                     /translation="MKRAVSILSAAALVACGVAFAVVALQAKDGAEERNGGTALAAAA
                     TDIGATNLPPKAIGRIREPETGYLGAQAVVREVDLYAHATSPEPMWSLPNPTHEGMPL
                     SFTVLDQEGDRLHVRLPIRPNGSTAWIDARQVVSYVVPNHLIVDLSDRTVTAQHGEET
                     LLSAGLAVGAPATPTPEGEFYVDISVADPGGSYGAWMLSIAGFSNVLSNFGGGIGQIA
                     FHGWSDESVIGQAVSNGCLRMQNGVIERLAPLAPVGTPVTIRA"
     gene            3495..>3710
                     /locus_tag="VFP02_10290"
     CDS             3495..>3710
                     /locus_tag="VFP02_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HET9543462.1"
                     /translation="MGMTAPTGHVDLVVEGMTCASCAARIEKRLNGLDGVAATVNFVT
                     ATASIEAPNGVTTDDLVAQVEAVGYGAH"
BASE COUNT          567 a         1334 c         1256 g          553 t
ORIGIN      
        1 acatcggcac caagcgctag acgcccagga cggtgtccag ggtcgtcgtg aggatcgccg
       61 cgccctcggc ggcttcctcg gcggtcaatg acagtggcgg ggcgatgcgc aggcagttgc
      121 cgtggaggcc gcccttgccg accagcaggc cgcgggccct gcagccctcg agcacggccg
      181 aggcgacctg gaggtcgggg gtcagcccgt cgtcgccgac gaactcgagg ccgatcatga
      241 ggcccttccc ccggatctcg ccgatggcgg ggtaccgctc gggcagcggg gccagggcgg
      301 ccatcagctc ggccccccgg gccttggtgt tggcctggag gccgtggtcg agcaggtagt
      361 ggaggttggc cagggcgccg gcgcaggaca gagggttgcc accgaaggtc gagatggagt
      421 tggccgacac cgagttcatg acctcggccc ggccgaccac gccggcgatg gtcagcccgt
      481 tgccgaggcc cttggcgaag gtcatcaggt cgggggtgat gccgtgggcc tgatggcccc
      541 agaaatggtc gccggtgcgg ccccagccgg tctggacctc gtcggcgatg aacagcatgt
      601 cgtgctcccg acacaccttc tcgaccgggc cgaagaagcc gtcggggggg atggcgaagc
      661 cgccgacgcc ctggacgggc tcggcgatca gggcggcgat gtcgcccgcg gtctgggtgg
      721 cgatcacgtc gcgcaggtcc tcggtgcagg cctcgatgaa gtcgccgtcg tccaggtggc
      781 ggaacgggct ccggaagcgg tagccggact gcatgtagct gacctggaac ggggtcaacg
      841 acgacggcga ccagccccgg ttcccggtga tgcccaccgt ggcgaacgag cgaccgtggt
      901 agctgttgcg cacggccacg atctggctcg accggcggaa caaggacgcc accagcaggg
      961 cggcctcgtt cgcctcggtc cccgacgtgg tgaagaacac ccgggggtcg gggatgccgg
     1021 acaggtcggc gatcatctcg gccagctcca cctgctggcg gatgaggtag agggtggagg
     1081 tgtggatcat ccgccccgcc tgctcgcgga tggcggcggt cacctcgggc acgtcgtagc
     1141 ccgtcatcgt cgtcaggatc ccgccgaaga agtcgaggta gcggttgcct tcgccgtcga
     1201 tgacgtgacg gccctggccc tcgaccagtt cgatgggctc gtcgtagtag agggcgagcc
     1261 acgagggcag gacggcccgg tgccgggcga ggagctcgtc gtgcgacatg gccgccacgg
     1321 taaaccagcc cggcggtggc cggtcctggc ggcgggtatc gtccgtttct gacgacccgt
     1381 cagaaatggc ttgagcagga gagccgcgtg gccgagatcc cgatgatcat cagcgtcgac
     1441 gaccacgtgg tcgagcccgc ccacctctgg cagacctggc tgcccgagaa gcaccgggcc
     1501 gacgccccct acgtcgagcg ccacggcctg ggcgggctca agtacgtcgg cggcaccacc
     1561 tacgagtacg agctgaccga cgacggcccg ccctgtgaca tctggttcta cgaggggaag
     1621 atgtaccccc acaagcgcca cgtcgcctcg gtgggcttcg agcgcgacga catgaccctg
     1681 tcgcccatca cctacgacga gatgcgaccg ggctgctacg agcccaaggc ccgggtcgac
     1741 gacatgctca tgaaccacgt cgaggcgtcg ctctccttcc cgaccttccc ccgcttctgt
     1801 ggccagacct tcgccgaggc ccgcgacaag gacctggcca tggcctgtgt gcgggcctac
     1861 aacgactgga tggtcgacga gtggtgcggc gacagcgacg gcgccctcat ccctctctgc
     1921 ttgatcccgc tctgggacat cgaggcggcg gcggcggaga tccaccgcaa tgccgcccgc
     1981 ggggtgcgag ccgtgtgctt cagcgagatc ccgtccaagc tcggcctccc cagcatccac
     2041 acgggccagt gggacccctt cttcctggcg tgccaggaga cccagaccgt cgtctgcatg
     2101 cacatcggct cgtcgtcgca gatgccggcc acctcgcccg acgccccgcc cgccgtggcc
     2161 gccacgctca gcttcaacaa cgccatggca tcgctgtcgg acttcctgtt ctcgggcgtg
     2221 ctggtgcgct tcccgaccct gacgctggcc tactccgagg gccagatcgg ttggctgccg
     2281 tacatgctcg agcgggccga cgacgtctgg cgcgagcacc gggcctgggg tggggtggcc
     2341 gacctggtcc ccgagccgcc gtcgacctac tactaccgcc aggtgtacgg ctgcttcttc
     2401 cgcgatcgcc acggcctcga cgccctcgac aaggtcggcg tggacaacat caccttcgag
     2461 acggactacc cccacaccga ctcgacctgg ccgaacacgt tggccgtcgc cgaggacctg
     2521 atgggccacc tgccccagga cgccgtcgac aagatctgcc ggggcaacgc ccaacgcatg
     2581 ctcgggttgg cgtagcggac ggtgaccctt ttctccccga cggtccttcc gtagcctgga
     2641 tcccgtgaag cgggccgtct cgatcctcag cgcggccgcc ctcgtggcct gcggtgtggc
     2701 ctttgccgtc gtggccctcc aggccaagga cggcgcggag gagcggaacg gcggcaccgc
     2761 cttggccgcc gcggccaccg acatcggggc gaccaacctc ccgccgaagg ccatcggtcg
     2821 catccgcgag cccgagaccg gctacctggg ggcccaagcc gtggtccgcg aggtcgacct
     2881 ctacgcccac gccacgtccc cggagcccat gtggtcgttg cccaacccca cccacgaagg
     2941 catgcccctc tcgttcaccg tcctggatca ggagggcgac cggctccatg tccgcctccc
     3001 catccgcccg aacggcagca cggcctggat cgacgcccgc caggtcgtca gctacgtggt
     3061 ccccaaccac ctgatcgtgg acttgagcga ccggacggtc acggcccagc acggtgagga
     3121 gaccctgctg tcggccggtc tcgccgtcgg ggccccggcc acgccgacgc cggagggcga
     3181 gttctacgtc gacatctccg tggccgaccc gggcggctcc tacggcgcct ggatgctcag
     3241 catcgcgggc ttctccaacg tcctcagcaa cttcggcggg ggcatcggtc agatcgcctt
     3301 ccacggctgg tccgacgagt ccgtgatcgg ccaagcggtc agcaacggct gcctccgcat
     3361 gcagaacggc gtcatcgagc gcctcgcccc cctcgcgccc gtcggcaccc cggtgaccat
     3421 cagggcatag acgtctgagt ggcttcgcag cgtcacgctc ggtccacgct gaactccgcg
     3481 ataggatacc ccccatgggt atgacggcgc cgaccggcca cgtcgacctc gtcgtcgagg
     3541 gcatgacctg cgcctcctgc gcggcccgca tcgagaagcg cctcaacggc ctcgacggcg
     3601 tggccgccac cgtcaacttc gtcaccgcga ccgcctccat cgaggccccg aacggcgtga
     3661 ccaccgacga cctggtcgcc caggtcgagg ccgtcgggta cggggcccac
//
LOCUS       DASTFV010000562         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2076362, whole genome shotgun sequence.
ACCESSION   DASTFV010000562 DASTFV010000000
VERSION     DASTFV010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2076362"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VFP02_10295"
     CDS             <1..1124
                     /locus_tag="VFP02_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9543463.1"
                     /translation="VESAVVHYGDTTLTFLNNLYRADQRGNSLSYASAVAVEEKSVID
                     YNRGNPDGVLQPGEEPRPPRVPLVAIYPEEGTLYSDNPFFLLDAEWVSDAEAEGAQLF
                     SEFVQEADNQERVLDYGFRPGNPEVAIGDPVSAENGVDPDQPQTLLEVPEPPVMIELL
                     DRWDEQRKPARVQLVIDISGSMGDAAGDDPVPTKLDLAIEAAVNALDLFADRDVVGLR
                     VFSTEIGPNGTDEVVDLVAPGPMTQEHRADLRDAISGLFPTNGTPLYTATQQAYDDAV
                     DGYDPASINAVVLLSDGVNDDGDATDDDDQLDELLATLSEGSEGQRAEPVRVFPISYG
                     ADADLNTLQEIAEASNAALYNASDPATIDRVLAAVISNF"
     gene            1130..1885
                     /locus_tag="VFP02_10300"
     CDS             1130..1885
                     /locus_tag="VFP02_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543464.1"
                     /translation="MWPLLARDAVVAGMGPTAILSAGAGGALGILVGGGLIGAITLAV
                     VFWLVAVAIPVGRRHLARRGPGSVDPFSLREPWRHAVQEALAAQARFRQATAGVAAGP
                     LQDRLTTIGAQLERGVAEAGRVARRGQTLEDVRRQIDTASILSELQRVEAVAQVSPTE
                     DNSLNRTAEALRAQLATAERIDGVLNETRDRLGLLDARLDEAVARSLELSVRTDDPDQ
                     LGGVNSTVDEVLEEMEALRLALDEADGDTPPTS"
     gene            1882..>2442
                     /locus_tag="VFP02_10305"
     CDS             1882..>2442
                     /locus_tag="VFP02_10305"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HET9543465.1"
                     /translation="MSVPGDQTPSGDEAPEDDARATAATTTTARLVRSSAVVGVGTGL
                     SRLSGLLRVGALTFALGATALSDTYNLANTTPNIIYELLLGGVLSASLVPIFVEHDQR
                     HDEDATNAVLTVAVLAMLALTVLALLAAPFIVDLYTLRLSSANAAAQAEVAVPMLRLF
                     VPQILFYGLMTLGTALLNARRSFFAPA"
BASE COUNT          361 a          949 c          795 g          337 t
ORIGIN      
        1 gcgtcgagtc ggcagtggtc cactacgggg acaccacgct gaccttcctc aacaacctct
       61 accgggccga ccagcggggc aactcgctgt cctacgcctc agccgtggcc gtcgaggaga
      121 agtcggtcat cgactacaac cggggcaacc ccgacggcgt cctccagccc ggcgaggagc
      181 cccgcccgcc ccgcgtcccg ctggtcgcca tctaccccga ggaagggacg ctctactcgg
      241 acaacccctt cttcctcctc gacgccgagt gggtgagcga tgccgaggcc gagggggctc
      301 agctcttcag cgagttcgtc caggaggccg acaaccagga acgggtcctg gactacggct
      361 tccgaccggg taacccggag gtggccatcg gcgatcccgt gtcggccgag aacggcgtcg
      421 accccgacca gccccagacc ctgctcgagg tccccgagcc cccggtcatg atcgagctcc
      481 tcgaccgctg ggacgagcag cgcaagccgg cccgggtgca gctcgtcatc gacatctccg
      541 gctccatggg tgacgccgcg ggtgacgacc ccgtgccgac caagctggat ctggccatcg
      601 aggccgcggt caacgccctc gacctgttcg ccgaccggga cgtggtcggg cttcgggtct
      661 tctccaccga gatcggcccc aacggcaccg acgaagtggt cgacctggtc gcgcccgggc
      721 cgatgaccca ggagcaccgg gccgacttgc gagacgcgat cagcggcctc ttccccacca
      781 acggcacgcc gctctacacc gccacccagc aggcctacga cgacgccgtc gacgggtacg
      841 acccggccag catcaacgcg gtggtcctgc tcagcgatgg cgtcaacgac gacggtgacg
      901 ccaccgacga cgacgaccag ctcgacgagc tgctggccac gctgtccgag ggcagcgagg
      961 gccagcgggc cgagccggtg cgggtcttcc ccatttccta cggcgccgac gccgacctga
     1021 acacgctcca ggagatcgcc gaggcctcca acgccgccct ctacaacgcc tccgacccgg
     1081 ccaccatcga ccgcgtcctg gccgccgtca tctccaactt ctgatcgccg tgtggcccct
     1141 gctcgcccgt gacgccgtag tggccggcat gggcccgacg gcgatcctgt ccgccggcgc
     1201 cggaggggcc ctggggatcc tggtcggcgg cggcctcatc ggggccatca cgcttgctgt
     1261 cgtgttctgg ctcgtcgccg tggccatccc cgtcggccgt cgccacctgg cccgacgagg
     1321 gccgggctcg gtcgatccgt tctcgctgcg tgaaccctgg cgtcacgccg tgcaggaggc
     1381 cctcgccgcc caggcccgct tccggcaggc caccgcgggc gtggccgcgg ggcctcttca
     1441 ggaccgcctc acgaccatcg gcgcccagct ggagcgaggg gtggccgagg ccgggcgggt
     1501 ggcccggcgg ggccagacgc tcgaagacgt ccggcgccag atcgacacgg cgtcgatcct
     1561 gtccgagctc caacgggtgg aggccgtggc ccaggtcagc ccgaccgagg acaacagcct
     1621 caaccgcacg gcggaggcgc tgcgggccca gttggccacc gccgagcgaa tcgacggggt
     1681 gctgaacgag acccgggacc gcctcgggct cctcgacgcc cgcctcgacg aggccgtagc
     1741 ccggtcactg gagctctcgg ttcgcaccga cgaccccgac cagctcggcg gcgtcaacag
     1801 caccgtcgac gaggtcctcg aggagatgga ggcattgcgc ctcgccctcg acgaggccga
     1861 cggggacacg cccccgacgt cgtgagcgtc ccgggcgacc agaccccgtc gggcgacgag
     1921 gcccctgagg acgacgcccg ggccaccgcg gccaccacca cgacggcccg cctcgtgcgg
     1981 tccagcgccg tcgtcggcgt cggcaccggg ctcagccgtc tgtcgggtct cctgcgagtc
     2041 ggggccctga ccttcgccct cggcgccacc gccctgagtg acacgtacaa cctggccaac
     2101 accaccccga acatcatcta cgagctcctg ctcggcggag tgctgtcggc cagcctcgtg
     2161 ccgatcttcg tcgagcacga ccagcgccac gacgaggacg ccaccaatgc cgtgctcacg
     2221 gtcgccgtcc tggccatgct cgccttgacg gtcctggccc tgctggccgc accgttcatc
     2281 gtcgacctgt acacgctgcg tctctcgtcg gcgaacgccg cggcccaggc cgaggtggcg
     2341 gtgccgatgc tgcgcctgtt cgtcccgcag atcctcttct acggactgat gacgctgggc
     2401 acggccctac tcaacgcccg gcggtccttc ttcgccccgg cc
//
LOCUS       DASTFV010000563         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2080044, whole genome shotgun sequence.
ACCESSION   DASTFV010000563 DASTFV010000000
VERSION     DASTFV010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2080044"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFP02_10310"
     CDS             <1..807
                     /locus_tag="VFP02_10310"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HET9543466.1"
                     /translation="KVDGGPEQVLATLADLEVDALVAIGGEDTLGVANRLHAEHGVAV
                     VGVPKTIDNDLSATEVTFGFNTAVQIATDAIDRLHTTAESHDRVMVCEVMGRHAGHIA
                     LWAGIAGGATMTLIPEEPFDIEAVCAAIVHRHEGGRYASIVVVAEGAVPKEGTLELVS
                     GEVDQFGHVRLGGIGQVLAEEIEKRTGYESRVTILGHVQRGGTPTAFDRVLSTRFGIA
                     AIDAVHDGDFGTMVALRADRIVRVPLAEGVGELKTVDPELYHGVAEVFFG"
     gene            815..2113
                     /locus_tag="VFP02_10315"
     CDS             815..2113
                     /locus_tag="VFP02_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9543467.1"
                     /translation="MDHLTAAIAEDRQRRLAGGLVARGWGDGDRVAVVAPSSANLLLT
                     VLAALRRGIVPVVLNAALTDGERDALVADADVVGQLGPAEMARLAEADPVDLAPHPRA
                     RPMHYTSGTTGKAKGVWSGVLPDAQAAALAAEEHELWEFSAGDRHLVCSPLHHSAPIR
                     FAAGTLLAGGEVIVLEKFTAAAFADAVATHAPTSSFMVPAHLQRLREAGSPPLDSFRL
                     VAHAGAPCPVPLKQWAVDQFPDGSVWEFYGATEGQFTACPAEEWVDRPGTVGRPRPGR
                     TLSVDEDGTIWCTVPDHARFTYWRDPGATARAWRDDAFTVGDLGRLDADGYLYLDGRR
                     DDLIISGGVNVYPFEVEQALHDLDGVEAVAVFGVPDERWGQRVCAAVVGSATPAEVQA
                     RARERLAAYKCPTAVFVVDDLPHTATGKLRRLDLADRFSD"
     gene            complement(2121..>2385)
                     /locus_tag="VFP02_10320"
     CDS             complement(2121..>2385)
                     /locus_tag="VFP02_10320"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HET9543468.1"
                     /translation="IPPDTTLLDSFVEDQVLTLDLSEQFTAVVGQRFIAAVAQIVYTA
                     TDLDGIEAVSFRVEGEDFPVNDENGAAVDEPVDRLDFVGLAPS"
BASE COUNT          320 a          832 c          888 g          345 t
ORIGIN      
        1 aaggtcgacg gcgggccgga gcaggtgctg gccaccttgg cggacctcga ggtcgacgcc
       61 ctggtcgcca tcggtggcga ggacaccctg ggcgtggcca accggctgca cgccgagcac
      121 ggcgtcgccg tcgtgggggt gcccaagacc atcgacaacg atctgtcggc caccgaggtc
      181 accttcgggt tcaacaccgc ggtgcagatc gccacggacg ccatcgaccg cctccacacc
      241 accgccgagt cccacgaccg ggtcatggtg tgcgaggtca tgggccgcca cgccggccac
      301 atcgcgctgt gggcggggat cgccggtggc gccacgatga ccctcatccc cgaggagccc
      361 ttcgacatcg aggcggtgtg cgccgccatc gtccaccgcc acgaaggcgg gcgctacgcc
      421 tcgatcgtcg tggtggccga aggcgcggtg cccaaggagg gcacgctcga gctcgtcagt
      481 ggcgaggtcg accagttcgg ccacgtacgc ctcgggggca tcggccaggt cctggccgag
      541 gagatcgaga agcgcacggg ctacgagtcg cgggtcacca tcctcggcca cgtgcaacgg
      601 gggggcacgc cgacggcctt cgaccgggtg ctctcgaccc gattcggcat cgccgccatc
      661 gacgccgtgc acgacggcga cttcggcacg atggtcgccc tgcgggccga ccggatcgtc
      721 cgggtcccgc tggccgaagg ggtgggggag ctgaagaccg tcgaccccga gctctaccac
      781 ggcgtggccg aggtcttctt cggctgatcg aggcgtggac cacctgacgg cggcgatcgc
      841 cgaggaccgc cagcggcgcc tggccggggg gctggtcgcc cgagggtggg gagatggtga
      901 ccgggtggcg gtcgtggcgc cgtcatcggc caacctcctg ctgaccgtgc tggccgcgct
      961 gcggcgcggg atcgtgcccg tcgtcctgaa cgcggccctg accgacggcg agcgggacgc
     1021 gctggtcgct gacgccgacg tggtcgggca gctgggaccg gcggagatgg ctcgactggc
     1081 ggaggccgat cccgtcgacc tggcccctca tccgcgggcc cggccgatgc actacacgtc
     1141 aggcacgacc gggaaggcca agggggtctg gtccggcgtg ttgcccgacg cccaggccgc
     1201 cgccctcgcg gccgaagagc acgagctctg ggagttctcg gccggcgacc ggcacctggt
     1261 gtgctcgccc ctgcatcact cggctccgat ccgcttcgcg gccgggacgc tgctggcggg
     1321 cggggaggtc atcgtgctcg agaagttcac ggctgcggcg ttcgccgacg ctgtggccac
     1381 ccacgccccg acctcgtcgt tcatggtccc cgcccacctc cagcgcctcc gggaggccgg
     1441 gtcaccgccg ctcgactcgt tccgcctggt ggcccacgcc ggggcgccgt gccctgtgcc
     1501 cctcaagcag tgggcggtcg accagttccc cgacgggtcg gtctgggagt tctatggcgc
     1561 caccgagggt cagttcacgg cctgtccggc ggaggagtgg gtcgaccgtc cgggcaccgt
     1621 cggtcgaccc cggccggggc gcacgctctc ggtggacgag gacggcacca tctggtgcac
     1681 cgtgccggac cacgcccggt tcacctactg gcgggacccc ggggccacgg cgcgggcctg
     1741 gcgggacgac gccttcacgg tgggcgacct cggtcgcctc gacgcggacg ggtacctgta
     1801 tctggacggt cggcgcgacg acctgatcat cagcggcggg gtcaacgtgt acccgttcga
     1861 ggtcgaacag gccctccacg acctcgacgg ggtcgaggcg gtcgccgtgt tcggtgtgcc
     1921 ggacgagcgt tggggtcagc gggtctgcgc cgccgtcgtc ggctcggcca cgccggccga
     1981 ggtccaggcc cgagctcgcg agcggttggc ggcctacaag tgcccgacgg cggtcttcgt
     2041 cgtggacgac ctgccccaca cggccacggg caagctccgc cgactcgatc tggccgaccg
     2101 gttctcggac tgagcgtcgc tcaggacggg gcgaggccga cgaagtcgag tcggtcgacg
     2161 ggctcgtcca cggcggcacc gttctcgtcg ttgacgggga agtcctcgcc ctcgacccgg
     2221 aagctgacgg cctcgatgcc gtcgaggtcg gtcgccgtgt agacgatctg ggccacggcg
     2281 gcgatgaagc gttgacccac gacggcggtg aactgctcgc tgaggtcgag ggtcaggacc
     2341 tggtcttcga cgaagctgtc gagcaaggtc gtgtccggcg ggatg
//
LOCUS       DASTFV010000564         2203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2080529, whole genome shotgun sequence.
ACCESSION   DASTFV010000564 DASTFV010000000
VERSION     DASTFV010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2080529"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="VFP02_10325"
     CDS             complement(<1..1277)
                     /locus_tag="VFP02_10325"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9543469.1"
                     /translation="MELTDDGARRPGLTPAPNDPRKLRDMLLVVVVCAAVALALVAVD
                     AVDHIYHALAARDATALLVGVILIPIGVSVFAFRRFQAAEEVWKETARLSMRDPLTGL
                     PTRRFLSQHFREVVRRVQRHGGRVAVFFCDLDKFKQINDTYGHEMGDQVMTKVAERMA
                     AIIEDDDILIRYGGDEFVVISPNAGTRPTAEAAAGRLIDALEAPFAVGEDKIQLSVCI
                     GVALTEEHCSEPDDVLRDADAAMYKAKAAGGGQVAIYDRSMANRITPSTAERRLRTAL
                     AEEQFRLYYQPVVSLQTQRMVKVEALIRWEDPERGIVSPGEFIPALENTGLIVPVGTW
                     VLEQACRQAHEWARTYSDQVPLMVTVNVSARQLAQADFGEVLRRVLGETGVDPGTICL
                     EITEGAVMADVDAAWTMLRSAKALGVYLALDDFG"
     gene            complement(1391..2065)
                     /locus_tag="VFP02_10330"
     CDS             complement(1391..2065)
                     /locus_tag="VFP02_10330"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9543470.1"
                     /translation="MTPPPVILAHGFAGSGRLTWGEAGWLELLADEGRSATAVDLLGH
                     GDAPKPREPEAYGAIAEHLAGLLPDEPVDGIGFSLGAATLLQVAMAEPSRFRRLVLAG
                     IGDDLFAPPDSEPVAALIEAGGELPESPFVRHIVDLASAPGTDRDAFVACLRRPRTPV
                     DEATLAAVDLPVLVVLGDRDHVAPADRLVAALPDARLQVLRGVDHGRTPGSLGFLDAA
                     LRFIAA"
BASE COUNT          334 a          773 c          806 g          290 t
ORIGIN      
        1 ccgaagtcgt cgagggcgag atagacgccg agggccttgg ccgagcgcag catcgtccag
       61 gcggcatcga cgtcggccat gaccgcgccc tcggtgatct cgaggcagat ggtcccgggg
      121 tcgaccccgg tctcgcccag cacccggcgc agcacctcgc cgaagtcggc ctgggccagc
      181 tggcgggccg acacgttgac cgtgaccatg agcgggacct ggtcgctgta ggtccgggcc
      241 cactcgtggg cctggcgaca ggcctgctcc aggacccagg tcccgacggg gacgatgagg
      301 ccggtgttct ccagggccgg gatgaactcg cccgggctga cgatgccccg ctcggggtcc
      361 tcccaacgga tgagggcctc caccttgacc atgcgttggg tctgcagcga caccaccggt
      421 tggtagtaga ggcggaactg ctcctcggcc agcgccgtgc gcaggcgccg ttcggcggtg
      481 gacggcgtga tgcggttggc catcgagcgg tcgtagatgg ccacctggcc gccacccgcg
      541 gcctttgcct tgtacatggc ggcatcggcg tcacgcagga cgtcgtcggg ctcggagcag
      601 tgctcctcgg tgagggccac cccgatgcac accgagagct ggatcttgtc ctcgccgacg
      661 gcgaagggcg cttcgagggc atcgatcaac cggcccgcgg cggcctcggc ggtgggtcgg
      721 gtgccggcgt tggggctgat cacgacgaac tcgtcacccc cgtagcggat gaggatgtcg
      781 tcgtcctcga tgatggcggc catgcgctcg gccaccttgg tcatcacctg gtcgcccatc
      841 tcgtggccgt aggtgtcgtt gatctgcttg aacttgtcga ggtcgcagaa gaacaccgcc
      901 acccggccac cgtgacgttg gacccggcga acgacctcgc ggaagtgctg gctcaggaag
      961 cgacgagtcg gcaaccccgt gagcgggtcc cgcatcgaga gacgggccgt ctccttccag
     1021 acctcctcgg cggcctggaa gcggcggaag gcgaacaccg acaccccgat ggggatgagg
     1081 atcacgccga cgagcagagc cgtcgcgtcc cgggcggcca acgcgtgata gatgtggtcg
     1141 acggcgtcga cggcaacgag ggccagagcc acggcggcac agacgacgac cacgagcagc
     1201 atgtcgcgga gcttgcgggg atcgttcggg gcgggtgtca gccccggccg tcgcgccccg
     1261 tcgtcggtca gctccatgac accgcctcca gcgccaccgg gcagcggtcc gcccagtgca
     1321 accctggtac ctccgtcggc cccgcccgag gatccttgag ccgcacgctc cgatgtccgg
     1381 agccggtcac tcaggccgcg atgaacctca gcgcggcgtc gaggaagccc aaggaccccg
     1441 gcgtccggcc gtgatccacc ccccgtagga cctggagacg ggcatcgggc agggccgcca
     1501 ccaaccgatc ggccggggcc acgtgatcgc ggtcgcccag cacgacgagc acggggagat
     1561 cgaccgccgc caaggtggcc tcgtcgacgg gcgtccgggg acgacgcagg caggccacga
     1621 acgcgtcacg gtcggtgccc ggcgccgagg ccaggtcgac gatgtggcgc acgaagggtg
     1681 actcggggag ctcgccgccg gcctcgatca gggcggccac gggctcggag tcgggtgggg
     1741 cgaagaggtc gtcgccgatc ccggcgagga cgaggcgccg gaaccggctc ggctcggcca
     1801 tggccacctg caggagggtg gccgcgccca gcgagaaccc gatgccgtcg accggctcat
     1861 cgggcaggag ccccgccagg tgctccgcta tggcgccgta ggcctcgggc tcccgcggct
     1921 tgggggcgtc gccgtgaccc agcaggtcga ccgccgtggc cgatcgcccc tcgtcggcca
     1981 ggagctccag ccagccagcc tcaccccacg tcaggcgacc cgagcccgcg aagccgtggg
     2041 ccaggatgac aggcgggggg gtcacgggct caggcccgcc agcgaagcgc ttcgtcggcc
     2101 agcacccgca atgtcccgag ggcggtggcc tcccaggtca gggtctcggc gcgtcggcgc
     2161 gccccctcgc ccaaccgggc gcggaggtcg gcgtcggtga gca
//
LOCUS       DASTFV010000565         2171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2087738, whole genome shotgun sequence.
ACCESSION   DASTFV010000565 DASTFV010000000
VERSION     DASTFV010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2087738"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VFP02_10335"
     CDS             <1..718
                     /locus_tag="VFP02_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543471.1"
                     /translation="GDRAAPVEAGSARAAVQRFLEARAVGDVAVAYGLLDQAGQKRYP
                     SPARFERAQADRAVVTAVRVGRERTTGAARADVTVTLSHPAAIDPFTGLVPARTVEGW
                     RASRQDGRWRVAADPVSVRADLPSDDRAPEAVTAWIQRLLGCDRQGAASLQVGAELYG
                     PADLAELPCQERGRWTVADPEGFDAAVEPEAYVAAFGPEVGSWARLVPVQGPRARFHV
                     VVGPLGDAWRVLGTDPDKAR"
     gene            780..>2171
                     /locus_tag="VFP02_10340"
     CDS             780..>2171
                     /locus_tag="VFP02_10340"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HET9543472.1"
                     /translation="MSKFRKLMTVGGLVATLALVTVVGTLAPSGASSHREALQISQDP
                     VADNTDTYAFVSPDKPDTVTLVGNWIPLEEPAGGPNFHNFGDDVKYTLNVDNDGDAVD
                     DIVWEFRFSTKVQNGNTFLYNTGPISSLKDPDFNIRQTYSVAKVENGRRTVIGSGLPV
                     PPANIGPRSTPNYDQLASAAVAGLSDGSKVFAGPRDDPFFVDLGSVFDLAGLRPFNQA
                     HLIPRPTEAGVDGVSGYNTHSIVLQVPIKQLTGDKKALTGSDDPDAVIGVYAASWRRQ
                     TKVLSPSGNDPLSLGQWVQVSRLGMPLVNEVVIPLGKKDRFNASEPADDAQFGKFVLD
                     PEVARLIPVLYPGVKVPAAPRNDIATIFLTGIPGLNQPKNVKASEMIRLNMGIAPSKT
                     PSPLGVLGGDNAGFPNGRRLADDTPDIELRALAGGTPFTPDFNIAPNNKLGDGVNTND
                     TPFLNQFPYVGTPH"
BASE COUNT          327 a          848 c          717 g          279 t
ORIGIN      
        1 cggcgaccgg gcggcgccgg tcgaggccgg gtcggccagg gcggccgtgc agcgcttcct
       61 ggaggccagg gccgtaggcg acgtcgccgt cgcctacggc ctgctcgacc aggcgggtca
      121 gaagcggtac ccgtcgccgg cccgcttcga gcgcgcccag gccgaccggg ccgtggtcac
      181 ggccgtgcgg gtcgggcgcg agcgcaccac cggggcggcc agggccgacg tcaccgtcac
      241 cctctcccac ccggcggcca tcgacccgtt caccgggctg gtccccgccc ggacggtcga
      301 gggatggcgg gccagccgcc aggacggccg ctggcgggtc gcggccgacc cggtgtcggt
      361 ccgcgccgac ctgcccagcg acgaccgcgc ccccgaggcg gtgaccgcct ggatccagcg
      421 cctgctcggc tgcgaccgcc agggggcggc cagcctgcag gtcggggccg agctgtacgg
      481 gccggccgac ctggccgagc tgccctgcca ggagcggggc cgctggacgg tggccgaccc
      541 cgagggcttc gacgccgccg tcgagccgga ggcctatgtc gccgccttcg gccccgaggt
      601 cggctcctgg gcccggctgg tgccggtcca gggcccgcgc gcccgcttcc acgtcgttgt
      661 cggcccgctg ggcgacgcct ggcgcgtgct cggcaccgac cccgacaagg cccgatgaga
      721 ggaggccccg agaccgtacg tccctgtcct gtcccatccg tcctcccgag gaggatcagt
      781 tgtccaagtt ccgcaagctc atgacggtgg ggggtctggt cgccacactg gcgctggtca
      841 ccgtcgtggg caccctggcg ccgtcggggg cgtccagcca ccgcgaggcc ctgcagatct
      901 cgcaggaccc ggtggccgac aacaccgaca cctacgcctt cgtcagcccc gacaagcccg
      961 acaccgtcac cctggtgggg aactggatcc cgctggagga gccggccggc gggcccaact
     1021 tccacaactt cggcgacgac gtgaagtaca cgctcaacgt cgacaacgac ggcgacgccg
     1081 tcgacgacat cgtgtgggag ttccgcttct cgaccaaggt ccagaacggc aacaccttcc
     1141 tgtacaacac cggtcccatc agctcgctga aggaccccga cttcaacatc cgccagacct
     1201 actcggtggc caaggtcgag aacggccgcc gcacggtcat cggctccggg ctgcccgtgc
     1261 cgccggccaa catcggcccg cgctcgacgc ccaactacga ccagctggcc tccgcggccg
     1321 tcgccggcct gagcgacggc agcaaggtgt tcgccggtcc ccgcgacgac ccgttcttcg
     1381 tcgacctggg ctcggtgttc gacctggccg gcctgcggcc cttcaaccag gcccacctga
     1441 tcccgcgtcc caccgaggcc ggggtcgacg gggtcagcgg ctacaacacc cactcgatcg
     1501 tcctccaggt ccccatcaag cagctcaccg gggacaagaa ggcgctcacc gggtccgacg
     1561 accctgacgc ggtcatcggc gtgtacgccg cctcctggcg ccgccagacc aaggtcctgt
     1621 cgcccagcgg caacgacccg ctcagcctcg gccagtgggt ccaggtctcg cgcctgggca
     1681 tgcccctggt caacgaggtc gtcatcccgc tcggcaagaa ggaccgcttc aacgccagcg
     1741 agcccgccga cgacgcccag ttcggcaagt tcgtcctcga ccccgaggtg gcccggctga
     1801 tcccggtgct gtacccaggg gtcaaggtgc cggcggcgcc gcgcaacgac atcgccacca
     1861 tcttcctgac cggcatcccc gggctcaacc agcccaagaa cgtgaaggcg tcggagatga
     1921 tccggctcaa catgggcatc gcgcccagca agacacccag cccgctgggg gtcctgggcg
     1981 gcgacaacgc cgggttcccg aacggtcgcc ggctcgccga cgacaccccc gacatcgagc
     2041 tgcgcgctct ggccgggggc accccgttca cgcccgactt caacatcgcg cccaacaaca
     2101 agctgggcga cggcgtgaac accaacgaca cgccgttcct caaccagttc ccgtacgtgg
     2161 gtacccccca c
//
LOCUS       DASTFV010000566         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2090548, whole genome shotgun sequence.
ACCESSION   DASTFV010000566 DASTFV010000000
VERSION     DASTFV010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2090548"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VFP02_10345"
     CDS             <1..1075
                     /locus_tag="VFP02_10345"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HET9543473.1"
                     /translation="KHPHQAVHLDLPLDDGDTSRYDVACRTLAEWRDEGVLIQDEDPS
                     FYVYRMGYKDDTGRLRQTAGVIGALELAQPGTGGVLPHEQTTPKAKSDRLEMLRFCKA
                     NLSAVWGLSTAEGLSGLCELPGPPVAAWTDGEGVHHRLWPISQPGIVEAIASAVSSEP
                     VVIADGHHRWSTSLQYRDERHEAGDGAGPWDLAMTYVVELVEEQLMVQPIHRLVNGLP
                     EGFDLAAALEASFELFDAGPVGPELLDRMDDAGALCLVTRDGAQLLRPRPEAMEGVRD
                     LDSSRLDQGLRAVPAHELTYQHGVENILAAVDSGRAQAGVLLRPATVEQIIDIAHGGE
                     LMPPKTTFFWPKPRTGQVFRLLD"
     gene            complement(1083..2108)
                     /locus_tag="VFP02_10350"
     CDS             complement(1083..2108)
                     /locus_tag="VFP02_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HET9543474.1"
                     /translation="MTDTDVPVTTKADWSSDQEVRWCPGCGDYSILTAVQMLMPELGV
                     RRENTVFVSGIGCAARFPYYMDTYGMHTIHGRAPAVATGLAMARPDLDIWVVSGDGDA
                     LSIGGNHLIHALRRNVNLNILMFNNQIYGLTKGQYSPTSEVGKVTKSTPFGSLDTAFN
                     PISLALGAEAGFVARTHDMDRKHMMETFRRAHAHQGGSFVEIYQNCNVFNDGAFEAVT
                     GKEQRASMLIPLVHGEQVRFGDDGERCVVLDAQGYARIAEVADVDEAQILVHDETRAD
                     PSLAFTLSRLASGPHEPTPVGVFRAVERPEYATEATQQIAAAQERSGPGVLKDLLHSG
                     ATWTVDA"
BASE COUNT          330 a          762 c          765 g          335 t
ORIGIN      
        1 caagcacccc caccaggccg tccacctcga cctcccgctc gacgacggcg acaccagccg
       61 gtacgacgtg gcctgtcgga cactggccga gtggcgggac gagggtgtcc tgatccagga
      121 cgaggacccg tccttctacg tctaccgcat gggctacaag gacgacacgg gtcgcctgcg
      181 ccagaccgcg ggggtcatcg gggccctgga gctggcccag cccggtaccg gcggcgtgct
      241 cccccatgag cagaccacgc ccaaggccaa gagcgatcgg ctggagatgc tgcggttttg
      301 caaggccaac ctctccgcgg tctggggcct gtccaccgcc gagggcctct ccggcctgtg
      361 cgagctgccc ggcccacccg tggccgcgtg gaccgacggc gagggggtcc accaccgcct
      421 ctggcccatc agccaacccg ggatcgtcga ggccatcgcg tcagccgtgt cctccgagcc
      481 cgtcgtcatc gccgacggcc accaccgctg gtcgacctcg ctgcagtacc gggacgagcg
      541 ccacgaggcc ggcgacggcg ccggaccctg ggacctggcc atgacctacg tggtcgagct
      601 ggtcgaggag cagctcatgg tccagcccat ccaccgtctc gtcaacggac tccccgaggg
      661 cttcgacctg gccgcggcgc tggaggcgtc cttcgagctg ttcgacgccg gcccggtcgg
      721 tcccgagctg ctcgatcgca tggatgacgc cggcgcgctc tgcctggtga cccgagacgg
      781 cgctcagctg ctccgtcctc gtcccgaggc catggaaggg gtccgggacc tggacagcag
      841 caggctcgac cagggcctac gagcggtccc cgcccacgag ctcacgtacc agcacggcgt
      901 cgagaacatc ctcgccgccg tcgactccgg ccgggcccag gccggcgtgc tgctccgtcc
      961 cgccaccgtc gaacagatca tcgacatcgc ccacggcggc gagctcatgc cccccaagac
     1021 caccttcttc tggcccaagc cgcgcactgg ccaggtcttc cgcctcctcg actgagtcga
     1081 gatcaggcgt cgacggtcca ggtggcgccg ctgtggagga ggtccttcag gacgccgggg
     1141 ccggaacgct cctgggcggc ggcgatctgc tgggtggctt cggtggcgta ctcggggcgc
     1201 tcgaccgccc ggaagacgcc gacgggcgtg ggctcgtgcg gtcccgaagc caggcgggac
     1261 agcgtgaagg ccaacgaggg gtcggcgcgg gtctcgtcgt ggaccaggat ctgggcctcg
     1321 tccacgtcgg ccacctcggc gatgcgggcg tagccctggg cgtcgaggac gacgcagcgc
     1381 tcgccgtcgt caccgaagcg gacctgctcg ccgtggacca gcgggatcag catggacgcc
     1441 cgctgctcct tgccggtgac cgcctcgaag gcgccgtcgt tgaagacgtt gcagttctgg
     1501 tagatctcga cgaaggagcc gccctggtgg gcatgggccc ggcggaaggt ctccatcatg
     1561 tgcttgcggt ccatgtcgtg ggtccgggcc acgaagccgg cctcggcccc gagggccagg
     1621 ctgatcgggt tgaacgccgt gtcgagagag ccgaagggcg tggacttggt gaccttgccc
     1681 acctcgctgg tcggcgagta ctggcccttg gtcaggccgt agatctggtt gttgaacatc
     1741 aggatgttca ggttcacgtt tcggcgcagg gcgtggatga ggtggttgcc gccgatggac
     1801 agggcatccc cgtcacccga gacgacccag atgtcgaggt cgggacgcgc catggccagg
     1861 ccggtggcca cggcgggggc gcgaccgtgg atggtgtgca tcccgtaggt gtccatgtag
     1921 tacgggaagc gggcggcaca gccgatgccg gacacgaaga ccgtgttctc ccgacgcacc
     1981 cccagttcgg gcatcagcat ctggacggcg gtgaggatcg agtagtcccc acagcccggg
     2041 caccagcgga cctcctggtc cgaggaccag tcggccttgg tcgtgacggg gacgtcggtg
     2101 tcggtcatca ggcgatcagt ccgtctccag ctcggccagg atggcccgct ccagctcacc
     2161 agaggtgaac ggcaggccct tcatcttcgt ga
//
LOCUS       DASTFV010000567         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2090654, whole genome shotgun sequence.
ACCESSION   DASTFV010000567 DASTFV010000000
VERSION     DASTFV010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2090654"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFP02_10355"
     CDS             complement(<1..341)
                     /locus_tag="VFP02_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543475.1"
                     /translation="MRPRHSLAGLVLVGVVVAAWAMVAMPGQATYGARVTADEPQYLL
                     SALSLGQDLDLDISDEIAARAYEPFHEVTLDPQTEANADGREFSPHDPLLPIILAGPM
                     AIGGWAAAKATL"
     gene            414..1058
                     /locus_tag="VFP02_10360"
     CDS             414..1058
                     /locus_tag="VFP02_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9543476.1"
                     /translation="MPSGQPIFAFEGVSLVTDGQSRLVDVDLTVADRAITVIVGASGS
                     GKSSLLRLGNRLEAPTQGVVRFRGEDLAALDIHAHRRQVGMLFQRPIPFAGTVRQNLQ
                     VAAPDLSDDDATALLERVTLAGSFLDRPADRLSGGEAQRMCLARSLATDPEVLLVDEA
                     TSSLDPDATRRLEELAVSLRDEGITVLWVTQEHEQIDRIADHAVRVAAGRVATA"
     gene            1055..1837
                     /gene="fetB"
                     /locus_tag="VFP02_10365"
     CDS             1055..1837
                     /gene="fetB"
                     /locus_tag="VFP02_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HET9543477.1"
                     /translation="MNGDVNALGLLASLVLIGVAVGISRWQRLGLEGSILVAVGRATV
                     QLLLVGFGLGLVLADDAPLLWSWLWVLGIILFAAVTIQRRAPAIPGLFRVALAGEVVT
                     AALSLTVIFGLSIFPLEARTLVPVAGMILGNALSSGVLAARRLVEEFTDKRDELEARL
                     ALGCSVPDASRPYLRVVLRTAMTPQIESTKAVGLVFLPGAMTGLVLAGVDPVDAVLVQ
                     LALMLLILGGVATISTVVTLGAGRRLFTPDERLVPLLREHHG"
     gene            1898..>2413
                     /locus_tag="VFP02_10370"
     CDS             1898..>2413
                     /locus_tag="VFP02_10370"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HET9543478.1"
                     /translation="MRLHAVDLHRVRLPLVRPFRTSFGEQTVREVLVIQAVTDVGEGW
                     GECAADVDPHYSPEFNDGARLALIDHLVPMLFAAGDNLSGAAVADLLEPVKGHRMAKE
                     ALEVAVLDAELRAAGVSLAEHLGGVRDAVDVGVSVGITDDPGVLAGWVAEYLDEGYRR
                     IKLKIEPGADIE"
BASE COUNT          300 a          907 c          840 g          366 t
ORIGIN      
        1 agcgtcgcct tcgccgccgc ccagcccccg atcgccatcg gcccggccag gatgatcggc
       61 agcagcggat cgtgggggct gaactcccgg ccgtcggcgt tcgcctcggt ctgggggtcg
      121 agggtgacct cgtggaacgg ctcgtaggcc cgcgctgcga tctcgtcgct gatgtccagg
      181 tccaggtcct ggcccaggct cagcgcggac agcaggtact gcggctcgtc ggcggtcacg
      241 cgggcgccgt aggtggcctg ccccggcatg gccaccatcg cccacgccgc cacgaccacg
      301 ccgaccagga ccagcccggc gagggagtga cggggacgca cgaggcgtca cggtacccac
      361 ccgtaaagcc cgcctaacgg gcccagggcc cggcaggcgg tcagactgca gcggtgccgt
      421 cgggccagcc gatcttcgcc ttcgaaggcg tcagcctggt caccgatggc caatcccggc
      481 tggtggacgt cgacctgacc gtcgccgatc gggccatcac ggtgatcgtc ggggcctcgg
      541 ggtcgggcaa gtcgagcctg ctccgcctcg ggaaccgcct cgaggccccg acccagggtg
      601 tcgtccgctt ccggggcgag gacctcgccg ccctcgacat ccatgcccac cggcggcagg
      661 tgggcatgct cttccagcgc ccgatcccct tcgccgggac cgtgcgccag aacctccagg
      721 tcgccgctcc ggacctgtcg gacgacgatg ccaccgctct cctggagcgg gtgaccctcg
      781 cggggtcgtt cctggaccga ccggctgacc gcctgtcggg cggcgaggcc caacgcatgt
      841 gcctggcccg ctcgctggcc accgaccccg aggtgctgct ggtcgacgag gccacctcgt
      901 cgctcgatcc cgacgccacc cgccgcctcg aggagttggc cgtctcgctc cgggacgaag
      961 gcatcaccgt gctctgggtc acccaggagc acgagcagat cgatcggatc gccgaccacg
     1021 ccgtgcgggt cgccgcgggt cgggtggcga ccgcatgaac ggcgacgtca acgcgctcgg
     1081 cctcctggcg tcactcgtcc tgatcggcgt ggccgtcggg atctcccgct ggcagcgcct
     1141 gggcctcgag ggctcgatcc tggtcgccgt gggccgggcc acggtccagc tcctcctcgt
     1201 cgggttcggg ctcggcctcg tcctggccga cgacgccccg ctgctgtggt cgtggctctg
     1261 ggtgctgggg atcatcctgt tcgccgcggt cacgattcag cgccgagccc cggccatccc
     1321 cggcctgttc cgcgtcgccc tcgccggcga ggtcgtgacc gccgcgctca gcctcaccgt
     1381 gatcttcggg ctctcgatct tcccgctgga ggcccgcacc ctggtccccg tcgcggggat
     1441 gatcctcggc aacgccctca gctcgggggt gctggccgcc cgccgactgg tcgaggagtt
     1501 caccgacaag cgggacgagc tggaagcccg tctggccctc ggctgctcgg tgccggacgc
     1561 ctcgcggcct tacctgcggg tggtcctgcg cacggccatg accccacaga tagagagcac
     1621 caaggccgtc gggctggtgt tcctgcccgg ggccatgacc gggctggtgc tggcaggggt
     1681 ggacccggtc gatgccgtcc tcgtccagct cgccctcatg ctcctgatcc tcggcggcgt
     1741 ggcgacgatc agcacggtcg tcaccctggg cgccggccgc cgactgttca cgccggacga
     1801 gcgtctggtc cccctcctcc gggagcacca cggctgactc gcccgcccgg cgacgaagag
     1861 cggcgcgagt gaccgaggcg ggtgcgatga tccgcccgtg cgcctccacg ccgtcgacct
     1921 ccaccgcgtc cgcctccccc tggtccgtcc cttccggacg tccttcggcg agcagaccgt
     1981 ccgcgaggtg ctggtgatcc aggccgtgac cgatgtcggc gagggctggg gcgagtgtgc
     2041 ggccgacgtc gatccccact actcccccga gttcaacgac ggtgcccgcc tggcgctgat
     2101 cgaccacctc gtgccgatgc tgttcgccgc cggcgacaac ctgtccggcg ccgccgtggc
     2161 cgacctcctc gagccggtca agggccaccg catggccaag gaggccctgg aggtggccgt
     2221 gctcgacgcc gagctacggg ccgcaggagt ctcgctggcc gagcacctcg ggggggtgcg
     2281 ggatgcggtc gatgtgggcg tgtccgtcgg gatcaccgac gaccccggcg tcctggccgg
     2341 atgggtggcc gagtacctcg acgagggcta ccgccgcatc aagctgaaga tcgaacccgg
     2401 cgccgacatc gag
//
LOCUS       DASTFV010000568         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2090939, whole genome shotgun sequence.
ACCESSION   DASTFV010000568 DASTFV010000000
VERSION     DASTFV010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2090939"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VFP02_10375"
     CDS             <1..254
                     /locus_tag="VFP02_10375"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET9543479.1"
                     /translation="ARWAGRRPWPERWRRATSALGPAAFVALAAPPVVLGSGSTTPTV
                     ARHGAVAVVLPLARVTRSTAAVLLAGLSTLWLITVLVGH"
     gene            complement(306..1061)
                     /locus_tag="VFP02_10380"
     CDS             complement(306..1061)
                     /locus_tag="VFP02_10380"
                     /EC_number="4.1.3.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HET9543480.1"
                     /translation="MAEPRFHSWIGSSTPDSITVGGLDLPSEVMGRLSLTELAYLLVT
                     HREPTAAERRMLDTVLVALADHGLTPSALATRLTHTGAPEAVQASVAAGLLGAGSVFL
                     GPTGDTAEFLAAVLAAAGPDPDLAVLADEAVAVRRAGGLRVPGLGHPVHRDVDPRTPR
                     LYELAEADGLLGPHLRLLTEVAAAHDRQSGKHLPINGAGAAGAALADLGLPPDVIRGF
                     VLIGRTAGLVAHIAEENQNPIGLPLWLEVEGRG"
     gene            complement(1054..>2001)
                     /locus_tag="VFP02_10385"
     CDS             complement(1054..>2001)
                     /locus_tag="VFP02_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9543481.1"
                     /translation="ADDRLMTTLPLFHINAPAYSTLGSIAAGAGLILLPAFSGSGFLD
                     DARRHGATQFNSIGAMLEILLRQPERADDADTPLRLCYTGPSPTEERQLEAEARFGLR
                     ITCGYALSETPYGTVWRHGTRPYGTLGAVRQHPTLGHVNDGRVMAEGTEVAVGGEGEL
                     ELRNPAIMKGYYEMPEETAAVVVDGWLKTGDIVRRNGDETYTFIGRKKEVIRRRGENL
                     SPAEVEAALERHPDVVEAAVIGVASDLSEEEVKAFVVPAAGVELDLPALKAHAAEHLA
                     RFKVPRWWEVVAELPHTATDRIAKHQLPTGRTEEEIDLG"
BASE COUNT          242 a          707 c          776 g          276 t
ORIGIN      
        1 cggcccggtg ggccgggcgt cggccgtggc ctgagcggtg gcggcgggcc accagcgccc
       61 tgggccccgc cgccttcgtg gcgctggccg ccccccccgt ggtcctcggg tcgggatcga
      121 ccacgccgac ggtcgcccgc cacggggccg tggccgtggt ccttcccctc gcccgcgtca
      181 cccggtccac cgcggccgtg ttgctggccg gcctgtccac gttgtggttg atcacggtcc
      241 tggtcggcca ttgagaggag caggagctga gcgccgaggc ctggctgctc tacgccgcca
      301 cctgatcagc cgcgcccttc gacctcgagc cagaggggga ggccgatggg gttctggttc
      361 tcctcggcga tgtgggcgac caggcccgcc gtgcggccga tgaggacgaa tccccggatg
      421 acgtcggggg gcaggccgag gtcggccaag gccgcgcccg cggcgcccgc gccgttgatc
      481 ggcaggtgct tgcccgattg gcgatcgtgg gcggcggcca cctcggtcag caggcggagg
      541 tggggaccga ggaggccgtc ggcctcggcc aactcgtaga ggcggggcgt gcggggatcg
      601 acgtcgcggt gcacgggatg gcccagaccg ggcacccgca ggcccccggc ccggcgaacg
      661 gcgaccgcct cgtcggcgag aacggccagg tcggggtcgg gcccggcggc ggccagcacg
      721 gcggccagga actcggcggt gtcgccggtg ggcccgagga agacactgcc cgcgcccagg
      781 agcccggcgg cgaccgaggc ctgcaccgcc tcgggggccc cggtgtgggt gaggcgggtg
      841 gccagcgcgc tgggcgtcag gccgtggtcg gccagcgcca ccagcacggt gtcgagcatc
      901 cgtcgctcgg ccgcggtcgg ctcccggtgg gtgaccagga ggtaggccag ctcggtcagc
      961 gacaaccggc ccatgacctc cgacggcagg tcgagcccgc ccacggtgat cgagtccggc
     1021 gtcgacgagc cgatccagga atggaatcga ggttcagcca aggtcgatct cctcctcggt
     1081 gcggcccgtg ggcagctggt gcttggcgat gcggtcggtg gcggtgtggg gcagctcggc
     1141 caccacctcc caccatcggg ggaccttgaa ccgagccagg tgctcggcgg cgtgggcctt
     1201 cagggccggg aggtcgagct caacgcccgc ggcggggacc acgaaggcct tcacctcctc
     1261 ctcggacagg tcggaggcga cgccgatgac ggcggcctcg accacgtccg gatggcgctc
     1321 gagggcggct tcgacctcgg ccgggctcag gttctcgccc cggcggcgga tgacctcctt
     1381 cttgcgcccg atgaacgtgt aggtctcgtc cccgtttcgg cgcacgatgt cgcccgtctt
     1441 cagccacccg tcgacgacca cggccgccgt ctcctccggc atctcgtagt agcccttcat
     1501 gatcgccggg ttgcgcagct ccagctcgcc ctcgccgccg accgcgacct cggtcccctc
     1561 ggccatgacc cggccgtcgt tgacgtggcc cagggtgggg tgctggcgga cggccccgag
     1621 cgtgccgtag ggccgggtgc cgtggcgcca gaccgtgccg tagggggtct cggacagggc
     1681 gtagccgcac gtgatgcgga gcccgaagcg ggcctcggcc tcgagctggc gctcctcggt
     1741 cggcgacggg cccgtgtagc agaggcgcag cggggtgtcg gcgtcgtcgg cccgctcggg
     1801 ctggcggagc aggatctcca gcatcgcccc gatggagttg aactgggtcg ccccatggcg
     1861 gcgggcgtcg tccaggaacc cgctcccgga gaacgcgggc agcaggatca ggcccgcccc
     1921 ggcggcgatc gagcccaacg tcgagtacgc cggggcgttg atgtggaaca gcggcagggt
     1981 ggtcatcagc cggtcgtcgg c
//
LOCUS       DASTFV010000569         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2092130, whole genome shotgun sequence.
ACCESSION   DASTFV010000569 DASTFV010000000
VERSION     DASTFV010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2092130"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..1478
                     /locus_tag="VFP02_10390"
     CDS             207..1478
                     /locus_tag="VFP02_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9543482.1"
                     /translation="MRDQPTDRLPVDPSTTPEAAAALTADWVVAHVPATWQEAAGRGG
                     AAAIRAVRPRAEYEAWYPTMAAAGLVAPTWPVAYGGLDVAPAVARAMEAELAPFNLGR
                     LNPLGLNLAAPALFAHGTEDQRRRFLPRIVDNTERWCQLFSEPGAGSDLASLATRAER
                     DGDTWVLTGQKVWTTWAHVSDFGVCLARTDAAVPKRKGITYFLVDLHAPGVTVRPLRH
                     ITGEIDFNEVFLDDVAVPDEHRVGEVGAGWSVANATLSGERQMVSGSGSGGVDRIGGS
                     GTEALIREARALGRWDEPLVRQALMAVYSEEQIRGWTNQRVRAGLKAGRPPGPESSIG
                     KVHQGALNQRIQVLATDLLGPAATAWTGAGKDYADALPRTVSGMLRSRANTIEGGTTE
                     VNKNILGERVLGLPREPDPWRDAPWGEVPRS"
     gene            1475..>2026
                     /locus_tag="VFP02_10395"
     CDS             1475..>2026
                     /locus_tag="VFP02_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET9543483.1"
                     /translation="MTAVGAGPYDTLVVERDGHIGWLIFDRPDRGNAMDAVMLAELER
                     AWQELDADPEVRVIVNTGNGSAFQTGLDVGQLASDPEALREQSRRTKRAELRFTAWHN
                     EVRKPVIAAVNGTCAGGGLHFVADADIVIVAEDATFLDPHVSIGQVTAYEAIALVRKS
                     PAEAVFRMALVGRHERLSARRAYD"
BASE COUNT          279 a          730 c          764 g          253 t
ORIGIN      
        1 acgcggcggc gtccgggctc gacgagtcga tgcgtcagga ggccttcgcc ctcgagctgt
       61 cgtcccgaag cgaggacttc cgcgagggct tcgccgcctt ccgggacaag cgggaccccg
      121 agttccgcgg tcggtgagcg gcgagccgat cagcgggccg atcaggcctg caccgcgatg
      181 agccgtcagg cgaagagcca tcgacgatga gggaccagcc caccgaccgc ctccctgtcg
      241 acccgtccac gacgcccgag gccgccgcgg ccctgacggc cgactgggtg gtggcccacg
      301 tcccggctac ctggcaggag gcggccgggc ggggtggggc ggcggcgatc cgggcggtgc
      361 gcccccgggc cgagtacgag gcctggtacc ccacgatggc ggccgccggg ctcgtggccc
      421 cgacctggcc ggtggcctac ggaggcctcg acgtggcccc ggccgtggcc cgggccatgg
      481 aagcggagct ggccccgttc aacctcggcc gcctgaaccc gctcggcctc aacctggccg
      541 cccccgccct gttcgcccac ggcacggagg accagcgccg gcgcttcctg cctcgcatcg
      601 tggacaacac ggagcgatgg tgccagctct tctccgagcc gggggcgggc tcggacctgg
      661 cgtcgctcgc cacccgggcc gagcgggacg gggacacctg ggtgctgacc ggtcagaagg
      721 tgtggaccac ctgggcccac gtgtccgact tcggggtctg cctggcccgg accgacgccg
      781 cggtgccgaa gcgcaagggc atcacctact tcctggtcga cctccacgcc cctggggtca
      841 ccgtgcgccc gctgcgccac atcaccggtg agatcgactt caacgaggtc ttcctggacg
      901 acgtggccgt gcccgacgag caccgcgtcg gcgaggtggg ggcgggctgg agcgtggcca
      961 acgccaccct ctccggggag cgccagatgg tgtcgggctc cggctcgggc ggggtcgacc
     1021 gcatcggggg ctcgggcacc gaggccctca tccgggaggc ccgggccctc ggccgctggg
     1081 acgagcccct ggtgcgccaa gcgctgatgg cggtctacag cgaggagcag atccggggct
     1141 ggaccaacca gcgggtgcgg gccggcctga aggcggggcg gccgccgggc ccggaatcgt
     1201 cgatcggcaa ggtccaccag ggcgccctga accagcgcat ccaggtcctg gcgaccgacc
     1261 tgctcggacc ggcggccacg gcgtggacgg gcgcggggaa ggactacgcc gacgcactgc
     1321 cccggaccgt gtcgggcatg ctgcgctcgc gggccaacac catcgagggt gggaccaccg
     1381 aggtgaacaa gaacatcctg ggggagcggg tcctgggcct gccccgcgag cccgacccct
     1441 ggcgggacgc cccctggggc gaggtccctc gctcgtgacc gccgtggggg ccgggcccta
     1501 cgacacgctg gtcgtcgagc gggacggtca catcggctgg ctgatcttcg accggcccga
     1561 ccggggcaac gccatggacg cggtgatgct ggccgagctg gagcgggcct ggcaggaact
     1621 ggacgccgac cccgaggtgc gggtcatcgt caacacgggg aacgggtcgg cgttccagac
     1681 cggcctcgac gtcggccagc tggcttcgga ccccgaggcg ctgcgcgagc agtcacggcg
     1741 gaccaagcgg gccgagctgc ggttcacggc ctggcacaac gaggtgcgca agccggtcat
     1801 cgccgcggtc aacgggacct gcgccggagg cggtctgcac ttcgtggccg atgccgacat
     1861 cgtgatcgtg gccgaggatg ccaccttcct cgacccccac gtctcgatcg ggcaggtcac
     1921 cgcgtacgag gccatcgccc tggtccggaa gtcgccggcc gaagccgtct tccggatggc
     1981 cctggtgggc cggcacgagc ggctgtcggc gcgccgggcc tacgac
//
LOCUS       DASTFV010000570         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2092806, whole genome shotgun sequence.
ACCESSION   DASTFV010000570 DASTFV010000000
VERSION     DASTFV010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2092806"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VFP02_10400"
     CDS             complement(<1..398)
                     /locus_tag="VFP02_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543484.1"
                     /translation="MILAELEVFHSRPIAPTRRVALGRQHLPLHPAPGFGGILLGGIA
                     AAHIGGLDPDLVPDLHRLIDHVEHGRRVAQPRLRYRFQTDRIGLTRSRLRLLGDGEQM
                     RFDFDAKAAPAQYLLGAVYAGAALPFADRRA"
     gene            complement(442..1143)
                     /locus_tag="VFP02_10405"
     CDS             complement(442..1143)
                     /locus_tag="VFP02_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543485.1"
                     /translation="MDEQPSEPTASDHAEVDYELDEWSTESRELLDRLLTTNDVNHAW
                     QGGTLSVAADHQGMVEELIGEVEATLLPALDPEAEKVVYEVAEWSPEDRADLTDALAD
                     EGVRYVFDDMGDLVVEAIDEDRVDTLIDELTEGEDVVDEDIPEATEVLSGLFVAADKL
                     RKSPRDGKAILAAREQAEAVIALPAPYGFERRTWAQIGLRAGALRVLLEEDAPDDEVV
                     SEAAEALRDLLHPMV"
     gene            1170..>2112
                     /gene="glpX"
                     /locus_tag="VFP02_10410"
     CDS             1170..>2112
                     /gene="glpX"
                     /locus_tag="VFP02_10410"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HET9543486.1"
                     /translation="MADERRELPDRNLALELVRVTEAAALAGSRWMGRGDKEGADGAA
                     VDAMRHVLNSVPMDGVVVIGEGEKDEAPMLFNGEAIGDGSPPQTDIAVDPIDGTTLTA
                     LGRGNALAVIAVSERGTMFDPGPCVYMEKIAVGPEAKGRVSLELSVTENLQAVAAAKG
                     ESVREVTAVVLDRPRHDELVAEIRGAGARIRLIPDGDVAGAISTAWPDSGADILFGIG
                     GTPEGVITAAALKCMGGEQQGRLWPRNDDERQAAIAAGYDLDRVLSIDDMVAGDNCFF
                     AATGITDGELLKGVHFAGDSVFTESLVMRSKSGTVRVV"
BASE COUNT          295 a          733 c          785 g          299 t
ORIGIN      
        1 gcccggcggt cagcgaaggg cagagcggcg ccggcgtaga ccgcgccgag caggtactgg
       61 gcgggggccg ccttggcgtc gaagtcgaag cgcatctgct cgccgtcgcc gagcaggcgg
      121 agccggctcc tggtcagacc gatgcggtcc gtctggaacc ggtagcgcag tcggggttgg
      181 gcgacccggc gcccgtgctc cacgtggtcg atgaggcgat ggaggtcggg cacgagatcg
      241 gggtcgaggc ccccgatgtg ggcggccgcg atccccccga gaaggatgcc gccgaagccc
      301 ggggcgggat ggaggggcag gtgctggcgg ccgagcgcga cccgccgggt gggcgcgatc
      361 gggcgcgagt ggaagacctc caactccgcc aggatcacgg cgtcaagcta cctgtggggg
      421 acgcggctcg tccctcgcgc tctagaccat cggatggagg aggtcgcgga gcgcttcggc
      481 ggcctcgctc acgacctcgt cgtcgggggc gtcctcctcc agcaggaccc gcagggcccc
      541 cgcccgcagg ccgatctggg cccaggtgcg gcgctcgaag ccgtagggcg cgggcagggc
      601 gatgacggcc tcggcctgct cgcgggcggc gaggatggcc ttgccgtccc gggggctctt
      661 gcgcagcttg tcggcggcga cgaagagccc ggacaggacc tcggtggcct cggggatgtc
      721 ctcgtcgacg acgtcctcgc cttcggtcag ctcgtcgatg agggtgtcga cccggtcctc
      781 gtcgatggcc tcgaccacga gatcgcccat gtcgtcgaac acgtaccgga cgccctcgtc
      841 ggccagggcg tcggtgaggt cggcccggtc ctcgggcgac cactcggcca cttcgtagac
      901 caccttctcg gcctccgggt cgagggcggg gagcagggtc gcctcgacct caccgatcag
      961 ctcctcgacc atcccctggt ggtcggccgc caccgagagg gtgcctccct gccaggcgtg
     1021 gttcacgtcg ttcgtggtca ggagccggtc gagcagctcc cgggactcgg tcgaccactc
     1081 gtcgagctcg tagtccactt cggcgtgatc gctcgcggtc ggctcactcg gctgctcgtc
     1141 catacacgag tggacggtaa cgtcgccgga tggccgatga acgacgcgaa ctgcccgacc
     1201 ggaaccttgc gctcgagctg gtccgggtca cggaggccgc cgcgctggct ggctcgcggt
     1261 ggatgggccg gggcgacaag gagggcgccg acggggccgc ggtggacgcc atgcgccacg
     1321 tgctcaacag cgtccccatg gacggcgtcg tcgtcatcgg ggagggcgag aaggacgagg
     1381 ccccgatgct gttcaacggc gaggccatcg gagacggctc gccgccacag accgacatcg
     1441 ccgtcgatcc catcgacggc accaccctga ccgccctcgg tcggggcaat gccctggccg
     1501 tgatcgccgt gtccgagcgg ggcacgatgt tcgacccggg cccgtgcgtc tacatggaga
     1561 agatcgccgt cggcccggag gccaagggcc gggtgtccct cgagctgtcg gtcaccgaga
     1621 acctgcaggc tgtcgccgcg gccaagggcg agtccgtccg cgaggtcacc gccgtggtcc
     1681 tggaccgccc ccgccacgac gagctcgtgg ccgagatccg aggggccggg gcccggatcc
     1741 gcctcatccc ggacggcgat gtggccgggg ccatctccac cgcctggccc gactccggcg
     1801 ccgacatcct gttcggcatc ggcggcaccc ccgagggggt catcaccgcg gccgccctca
     1861 agtgcatggg gggcgagcag caggggcgtc tctggccgcg caacgacgac gagcgccagg
     1921 cggccatcgc tgcgggctac gacctcgacc gcgtcctgtc gatcgacgac atggtggccg
     1981 gggacaactg cttcttcgcc gccaccggaa tcacggacgg cgagctgctc aagggcgtgc
     2041 acttcgccgg cgactcggtc ttcaccgaat cgctggtgat gcgctcgaag agcggcaccg
     2101 tgcgcgtggt ca
//
LOCUS       DASTFV010000571         7249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2093492, whole genome shotgun sequence.
ACCESSION   DASTFV010000571 DASTFV010000000
VERSION     DASTFV010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2093492"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /gene="atpB"
                     /locus_tag="VFP02_10415"
     CDS             <1..736
                     /gene="atpB"
                     /locus_tag="VFP02_10415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET9543487.1"
                     /translation="HVIEWPEFFLEGTPFAVSKVVVLMWISALISVGFVILAGRNFKM
                     VPRPLQNVGESLYGFIQDGIILETMGPDGLKYTSFLGSLFVFVLSCNIWGIIPGAQMP
                     VNARIALPFFLAMMVWVIFVVVGVKKQGPLTYLKNTGFPPGVPWPLYFLVTPIELVST
                     FIVRPLSMFVRLFANMLAGHLLLTSFAVICAALFSLTLSLIILPFSFGLLAALTGFEI
                     LVAFLQAFIFTILAAVYIGSSMHPEH"
     gene            745..1008
                     /gene="atpE"
                     /locus_tag="VFP02_10420"
     CDS             745..1008
                     /gene="atpE"
                     /locus_tag="VFP02_10420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HET9543488.1"
                     /translation="MLDILTIAAQAEGELEAGLTAVGRGIVYGGAAIGPGIGIGIVVG
                     NAITAMARQPEAAGTVRTTMFLGIAFTEALALFGFVLAFIISG"
     gene            1016..1672
                     /gene="atpF"
                     /locus_tag="VFP02_10425"
     CDS             1016..1672
                     /gene="atpF"
                     /locus_tag="VFP02_10425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET9543489.1"
                     /translation="MRTRLLLSGVALTLVALFGWTGTAHAASEEELREECIHILEEGG
                     EVDDCQEAPSPILPEPNELFWGALSFLVVFGALAKFGFPPIKKGLEDRAAKIQGDLDE
                     AEAAKAEVTSKAAEYDAKLAEARSDAARIIEEARQDADAYRSDKKTEADAEIARLKAQ
                     AQADVEAAKAQAIGDLRGEVATLAIGAAEHVVGQSLDRDANVALVEQFIDSVGAKGSN
                     "
     gene            1672..2214
                     /gene="atpH"
                     /locus_tag="VFP02_10430"
     CDS             1672..2214
                     /gene="atpH"
                     /locus_tag="VFP02_10430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HET9543490.1"
                     /translation="MAEASRSLAYAAAMYAVANAEGQLSSVGDELFAVARAIEGNDAL
                     RDALGDPHLPAERRQQIIEDLAGSRTSDVTLGLASMVVAAGRGRELPDIVDALLQLSA
                     EDQGRQVAEVRSAIELSEDQQERLATALKSATGREVDVRVVVDPTVVGGLVVRVGDQV
                     IDGTVRHRLSQLRESLSARS"
     gene            2248..3897
                     /gene="atpA"
                     /locus_tag="VFP02_10435"
     CDS             2248..3897
                     /gene="atpA"
                     /locus_tag="VFP02_10435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET9543491.1"
                     /translation="MADLNIDTTDITAALRKNLDGYTPDLSVTQVGRVLEVGDGIARV
                     SGLPDCAVNELLEFDGGTVGLALNLDEESIGAVILGEGEQIAAIEEGSSVQATGRIQT
                     VPVGDALIGRVVNALGEPIDGAGPVNAELTRRMEIQAPGITRRKPVHEPMQTGIKAID
                     AMTPIGRGQRELIIGDRKTGKTTICVDTILNQAGQGVKCIYVAIGQKSSTVAQTVNTL
                     REHGAMEYTVVVCAPAADPAPFKYIAPYAGCAMGQHWMENGEHALVVYDDLSKQAEAY
                     RQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLSDAEGAGSLTALPVIETKAGDVSA
                     YIPTNVISITDGQVYLQDDLFKSGVRPAVDVGISVSRVGGDAQIKAMKSVSGTLKLDL
                     AQFRELEAFATFGSELDKVSAAQLDRGYRLTELLKQNLNSPMPVEEQVVSIFAGTNGY
                     LDDIGVEDVKRFESELLDFVKTRHGIILETIRDKGTLPEEDGLDTAVKGFAEQFQPTV
                     DVAPEPDAEAQADAETTLAGGRAEATLPEVEIARDDDEDDE"
     gene            3904..4878
                     /locus_tag="VFP02_10440"
     CDS             3904..4878
                     /locus_tag="VFP02_10440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004145.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET9543492.1"
                     /translation="MAGGQERILRRRISATESTKKITRAMELIAATRVVQAQERTRKA
                     RPYAEQITNVILDLAGAGASSSHPLLAERPGGGKAAFVVVTSDRGLAGAYNSAVIRAA
                     EREIQAARTEGLDYSLFLVGKKALTYFRFRGYRIDHVFLSKTDRPTYEDAREIGQAVR
                     TAFEGGELDRVDLVYTRFISAGSQRVVVRRFLPLEIDDADDSNAAPRADFEFEPSPDG
                     ILDELVPRYVEARLYAALLDASASEHASRQRAMKAATDNAEELIIALSRAMNRARQDA
                     ITTEIMEIVGGAEALAQTGAGEDDLLPDHILSSHLFPAHHSPDHRRAS"
     gene            4906..6345
                     /gene="atpD"
                     /locus_tag="VFP02_10445"
     CDS             4906..6345
                     /gene="atpD"
                     /locus_tag="VFP02_10445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET9543493.1"
                     /translation="MTVTESSTGTELKDGRIVGIAGPVIDVEFPRGHLPEINTALELD
                     VTMDGESSKIVAETAQQIGDSRVRAICLRATDGLTRGAPVRNLGHGIEMPVGEAVLGH
                     VFNVIGQSLDTDSVEGIEDHWPIHRDAPDFDSLEPKAVVFETGIKVIDLLTPYVQGGK
                     IGLFGGAGVGKTVIILELINRVATQHGGVSVFGGVGERTREGTDLWIEMQESGVIEKA
                     ALVYGQMDEPPGVRLRVALSALTIAEYFRDVKQQDVLLFIDNIFRFVQAGSEVSTLLG
                     RMPSAVGYQPTLADEMGELQERITSTRGRSITSIQAVYVPADDYTDPAPFTTFTHLDA
                     TTELSRQIASLGIYPAVDPLASSSSILAPEVVGARHYEVARRVQEVLQRYKELQDIIA
                     ILGLDELSDEDRIIVDRARKVQRFLSQPFFVGEVFTGLKGIHVPTETTVDSFEALVDG
                     ELDDLPEQAFLNVGDADSARAKAAELTAD"
     gene            6345..6608
                     /gene="atpC"
                     /locus_tag="VFP02_10450"
     CDS             6345..6608
                     /gene="atpC"
                     /locus_tag="VFP02_10450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HET9543494.1"
                     /translation="MLQVELVSPEEILFSGEAYMVVVRTIGGGEIAFLPGHAPFLGAL
                     GEGEARLHLQNGGEVKSLHLNGGFVEVSHDKVTILSDEAEVVE"
     gene            complement(6618..7022)
                     /locus_tag="VFP02_10455"
     CDS             complement(6618..7022)
                     /locus_tag="VFP02_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543495.1"
                     /translation="MHVRHLAPVLALLLLLGACGGDDSGTVGDAASTSITTPSETTAA
                     PGTVAGPSAGEACTDLAERYVRRARTMFDVEGTPSDALVDRVRARLVEFDQIAATAGC
                     GEEYVTAVCEGLDALTQEGILVIIPLTTAQCL"
     gene            7057..>7249
                     /locus_tag="VFP02_10460"
     CDS             7057..>7249
                     /locus_tag="VFP02_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HET9543496.1"
                     /translation="MPRRRLSVVLLVPPPVAQEVDGLRRALGDGVLGRVPPHITLIPP
                     INVREEDLADAFAVVRRAAA"
BASE COUNT         1138 a         2634 c         2413 g         1064 t
ORIGIN      
        1 ccacgtcatc gagtggcccg agttcttcct cgagggcacg ccgttcgcgg tcagcaaggt
       61 cgtcgtcctc atgtggatct cggcgctgat ctcggtcggc ttcgtgatcc tggccggccg
      121 caacttcaag atggtccccc gcccgctgca gaacgtgggc gagtcgctct acggcttcat
      181 ccaggacggc atcatcctgg agacgatggg ccccgacggc ctgaagtaca cgtcgttcct
      241 cgggtcgctg ttcgtcttcg tgctcagctg caacatctgg gggatcatcc ccggtgccca
      301 gatgccggtg aacgcccgga tcgcgctgcc cttcttcctg gcgatgatgg tgtgggtcat
      361 cttcgtggtg gtcggcgtca agaagcaggg cccgctcacg tacctgaaga acacgggctt
      421 cccgccgggc gtgccgtggc cgctctactt cctggtgacg cccatcgagc tggtctcgac
      481 gttcatcgtg cggccgctgt cgatgttcgt ccggctcttc gccaacatgc tggccggcca
      541 cctgctgctc accagcttcg ccgtcatctg cgccgcgctg ttcagcctga cgctgtcgct
      601 gatcatcctg ccgttctcgt tcggcctgct cgccgcgctc accgggttcg agatcctggt
      661 ggccttcctg caggccttca tcttcaccat tctcgctgcc gtgtacatcg gtagctcgat
      721 gcatccagag cactaggagg aaccgtgctg gacatcctga ccatcgccgc ccaagcggaa
      781 ggcgaactcg aggccggcct gaccgccgtc ggacgaggca tcgtctacgg cggcgcagcc
      841 atcggccccg gcatcggcat cggcatcgtg gtgggcaacg ccatcaccgc catggcccgt
      901 cagccggaag ccgcgggcac ggtgcgcacc accatgttcc tgggcatcgc cttcaccgag
      961 gcgctcgcgc tgttcggctt cgtcctcgcc ttcatcatca gcggctgagg tcgtcatgcg
     1021 aacccgactg ctgctgagcg gggtggccct caccctcgtc gccctcttcg ggtggaccgg
     1081 gacggctcac gccgccagcg aggaagagct ccgtgaggag tgcatccaca tcctcgagga
     1141 gggtggggag gtcgacgact gccaagaggc gccgagcccc atcctgcccg agcccaacga
     1201 gctgttctgg ggcgccctgt cgttcctggt cgtgttcggt gcgctggcca agttcggctt
     1261 cccgcccatc aagaagggcc tcgaggaccg tgccgccaag atccagggtg acctggacga
     1321 ggccgaggcg gccaaggccg aggtgaccag caaggcggcg gagtacgacg ccaagctggc
     1381 cgaggcccgg agcgacgcgg cccggatcat cgaagaggcc cgccaggacg ccgacgccta
     1441 ccggtcggac aagaagaccg aagcggacgc ggagatcgcc cggctgaagg cgcaggcgca
     1501 ggccgacgtc gaggcggcga aggcccaggc gatcggcgac ctccgtggcg aggtggccac
     1561 gctggccatc ggcgcggccg agcacgtcgt gggccagagc ctggaccgcg acgccaacgt
     1621 ggcgctggtc gagcagttca tcgactcggt cggggccaag ggctcgaact gatggccgaa
     1681 gccagccgct ccctcgccta cgccgcggcg atgtacgcgg tcgccaacgc cgagggccag
     1741 ctgtcctcgg tgggcgacga gctcttcgcc gtggcccggg ccatcgaggg caacgacgca
     1801 ctgcgtgacg ccctgggtga tccccacctg ccggcggagc gacgccagca gatcatcgag
     1861 gacctcgccg gctcccggac ctccgacgtg accctcggcc tggcctccat ggtcgtggcc
     1921 gccggtcgcg ggcgggagct gcccgacatc gtcgatgcgc tgctgcagtt gtccgccgag
     1981 gaccagggtc gccaggtggc cgaggtgcgc tcggccatcg agctctccga ggaccagcag
     2041 gagcggctcg ccacggccct gaagtccgcc accgggcgcg aggtggacgt ccgcgtcgtc
     2101 gtcgacccca ccgtggtcgg cggcctggtg gtccgggtgg gcgaccaggt catcgacggc
     2161 acggtccgcc accgcctgtc ccagctccgc gaatccctgt ccgcccgctc ctgagcccca
     2221 acgaggaaca agaggaagac gccaaccatg gctgatctga acatcgacac cacggacatc
     2281 accgccgccc tgcgcaagaa cctcgacggc tacacaccgg acctctcggt cacccaggtc
     2341 ggccgcgtcc tcgaggtcgg cgacggcatc gcgcgggtgt cgggcctgcc cgactgcgcc
     2401 gtcaacgagc tcctggagtt cgacgggggc accgtcggcc tcgccctgaa cctggacgag
     2461 gagtccatcg gcgccgtcat cctgggtgaa ggcgagcaga tcgccgccat cgaggagggc
     2521 tcctcggtgc aggcgaccgg tcgcatccag accgtccccg tcggcgacgc cctgatcggc
     2581 cgggtggtca acgccctcgg cgagcccatc gacggcgccg ggccggtgaa cgccgagctc
     2641 acccgccgca tggagatcca ggccccgggc atcacccggc gcaagccggt ccacgagccg
     2701 atgcagaccg ggatcaaggc catcgacgcc atgacgccga tcggccgggg ccagcgcgag
     2761 ctgatcatcg gcgaccgcaa gacgggcaag acgaccatct gcgtcgacac gatcctgaac
     2821 caggccggcc aaggcgtgaa gtgcatctac gtggccatcg gccagaagag ctcgacggtc
     2881 gcccagacgg tcaacacgct ccgcgagcac ggcgccatgg agtacacggt cgtggtctgc
     2941 gccccggccg ccgatccggc gccgttcaag tacatcgccc cgtacgccgg ctgcgccatg
     3001 ggccagcact ggatggagaa cggcgagcac gccctggtcg tctacgacga cctctccaag
     3061 caggccgagg cctaccgcca gatgtccctg ctgctgcgcc gcccgccggg ccgcgaggcg
     3121 taccccggcg acgtcttcta cctccacagc cgcctgctgg agcgggctgc caagctctcc
     3181 gacgccgagg gcgccgggtc actgacggcc ctgccggtca tcgagaccaa ggcgggcgac
     3241 gtgtcggcct acatcccgac caacgtgatc tcgatcaccg acggccaggt gtacctccag
     3301 gacgacctct tcaagtccgg tgtgcgtccc gcggtcgacg tgggcatctc ggtgtcccgc
     3361 gtgggcggtg acgcccagat caaggccatg aagtcggtgt cgggcacgct caagctcgac
     3421 ctggcccagt tccgtgagct ggaggccttc gccacgttcg gctccgagct ggacaaggtc
     3481 tcggccgccc agctcgaccg cggctaccgc ctgaccgagc tgctcaagca gaacctgaac
     3541 tcgcccatgc cggtcgagga gcaggtcgtg tcgatcttcg ccggtacgaa cggttacctc
     3601 gacgacatcg gggtcgagga cgtgaagcgc ttcgagtccg agctcctcga cttcgtcaag
     3661 acccgccacg gcatcatcct cgagacgatc cgggacaagg gcacgctgcc cgaggaggac
     3721 ggcctcgaca ccgccgtcaa gggcttcgcc gagcagttcc agcccacggt cgacgtggcc
     3781 ccggagcccg acgccgaagc ccaggccgac gccgagacca ccctggccgg tggccgggcc
     3841 gaggccacgc tgcccgaagt cgagatcgcc cgggacgacg acgaggacga cgagtaggcc
     3901 cgcatggctg gtggccagga acgcatcctc cggcgtcgga tctcggcgac cgagtcgacg
     3961 aagaagatca cgcgcgccat ggagctcatc gccgcgaccc gggtggtcca ggcccaggag
     4021 cgcacccgca aggcccggcc ctacgccgag cagatcacca acgtgatcct cgacctggcc
     4081 ggcgccggcg cctccagcag ccatcccctc ctggccgagc gtccgggtgg gggcaaggcc
     4141 gccttcgtgg tcgtcacctc ggaccggggc ctggccgggg cctacaactc cgcggtcatc
     4201 cgggccgccg agcgggagat ccaagcggct cgcaccgagg gactcgacta ctcgctgttc
     4261 ctcgtcggga agaaggcgtt gacctacttc cgcttccggg gctaccgcat cgaccacgtc
     4321 ttcctgtcca agaccgaccg gccgacctac gaggacgccc gcgagatcgg ccaggccgtg
     4381 cgcaccgcct tcgagggcgg tgagctggac cgggtcgacc tcgtgtacac ccgcttcatc
     4441 agcgccggga gccagcgggt cgtggtccgt cgcttcctgc ccctcgagat cgacgacgct
     4501 gacgactcga acgccgcccc gcgggcggac ttcgagttcg agccctcgcc cgacggcatc
     4561 ctcgacgagc tggtgccccg ctatgtcgag gcccgcctct acgccgccct gctcgacgcc
     4621 tcggcgtccg agcacgcctc ccgacagcgg gccatgaagg ccgccaccga caacgccgaa
     4681 gagctcatca tcgccctgtc gcgggccatg aaccgggccc gccaggacgc catcacgacc
     4741 gagatcatgg agatcgtggg cggggccgag gcgctggccc agacgggcgc cggcgaagac
     4801 gacctcctgc ccgatcacat cctgagctcg cacctgttcc ccgcccacca ttcccccgat
     4861 caccgccggg cttcgtgagc cccgccccag aggagacctg aaccgatgac cgtgaccgag
     4921 tccagtaccg gcaccgaact gaaggacggc cgcatcgtcg gcatcgccgg gccggtgatc
     4981 gacgtcgagt tcccccgggg ccaccttccc gagatcaaca ccgcgctcga gctggacgtc
     5041 accatggacg gcgagtccag caagatcgtg gccgagacgg cccagcagat cggcgacagc
     5101 cgggtccggg ccatctgcct gcgggccacc gacggcctca cccggggcgc ccccgtgcgg
     5161 aacctgggcc acggcatcga gatgccggtc ggggaggccg tgctcggcca cgtgttcaac
     5221 gtgatcggcc agtccctcga caccgactcg gtcgaaggca tcgaggacca ctggcccatc
     5281 caccgcgacg ccccggactt cgactccctc gagcccaagg ccgtcgtctt cgagaccggc
     5341 atcaaggtca tcgacctcct gaccccctac gtgcagggtg gcaagatcgg cctgttcggt
     5401 ggggccggcg tcggcaagac cgtcatcatc ctcgagctca tcaaccgggt cgccacccag
     5461 cacggtggcg tgtcggtgtt cggtggcgtg ggggagcgga cccgggaggg caccgacctc
     5521 tggatcgaga tgcaggagtc gggcgtcatc gagaaggccg ccctggtcta cggccagatg
     5581 gacgagcccc cgggcgtgcg cctgcgggtc gccctgtcgg ccctgaccat cgccgagtac
     5641 ttccgggacg tgaagcagca ggacgtgctg ctgttcatcg acaacatctt ccgcttcgtg
     5701 caggccggct ccgaggtgtc caccctcctc ggtcgcatgc cctcggccgt gggctaccag
     5761 cccacgctgg ccgacgagat gggcgagctg caggagcgca tcacctcgac ccggggtcgg
     5821 tccatcacct cgatccaggc cgtgtacgtc cccgccgacg actacaccga cccggcgccg
     5881 ttcacgacgt tcacgcacct cgatgccacc accgagctgt cccgccagat cgcctcgctg
     5941 ggcatctacc cggctgtgga cccgctggcc tccagctcgt cgatcctggc ccccgaggtc
     6001 gtcggcgccc gccattacga agtggcccgc cgggtgcagg aggtgctgca gcgctacaag
     6061 gagctgcagg acatcatcgc catcctcggc ctcgacgagc tgtcggacga ggaccggatc
     6121 atcgtggacc gggcccgcaa ggtgcagcgc ttcctgagcc agcccttctt cgtgggtgag
     6181 gtcttcacgg gcctgaaggg catccacgtg cccaccgaga ccacggtcga ctccttcgag
     6241 gcgttggtcg acggcgagct ggacgacctg cccgagcagg ccttcctgaa cgtcggcgac
     6301 gcggacagcg cccgggccaa ggccgccgag ctcaccgcgg actgatgctc caggtcgagt
     6361 tggtgtcccc ggaggagatc ctcttctccg gcgaggccta catggtcgtc gtgcgcacca
     6421 tcggaggcgg cgagatcgcc ttcctgcccg gccacgcccc gttcctgggg gcgctcggtg
     6481 agggcgaggc ccgcctccac ctccagaacg gtggcgaggt caagagcctc cacctcaacg
     6541 gcggcttcgt cgaggtcagc cacgacaagg tcaccatcct cagcgacgag gccgaggtcg
     6601 tcgagtagcg cctccgctca gaggcactgg gcggtggtca gcgggatgat gaccaggatt
     6661 ccctcctggg tgagggcgtc gaggccttca cacaccgcgg tcacgtactc ctcgccgcac
     6721 cccgccgtgg ccgcgatctg gtcgaactcg accagccggg cccggacgcg gtcgaccaga
     6781 gcgtccgacg gcgtgccctc cacgtcgaac atcgtgcggg cccgccggac gtagcgctcg
     6841 gccaggtcgg tgcaggcttc gccggcggaa gggccggcca ccgtgcccgg ggcggccgtg
     6901 gtctccgagg gggtggtgat gctggtcgac gcggcgtcgc ccacggtccc gctgtcatcg
     6961 ccgccacagg ctccgaggag caggagcagg gcgaggacag gggccagatg acgaacgtgc
     7021 acggattcgg gaacctagcg cccctaccgt gcgggtatgc cgcgccgccg actctcggtc
     7081 gtgctcctgg tcccgccgcc ggtcgcccag gaggtggacg gcctgcgccg ggctctcggg
     7141 gacggcgtcc tcggccgggt cccgccccac atcaccctga tcccgccgat caacgtccgc
     7201 gaggaggacc tggccgacgc cttcgccgtg gtccggaggg cggcagccg
//
LOCUS       DASTFV010000572         4339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2099578, whole genome shotgun sequence.
ACCESSION   DASTFV010000572 DASTFV010000000
VERSION     DASTFV010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2099578"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VFP02_10465"
     CDS             complement(<1..1166)
                     /locus_tag="VFP02_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543497.1"
                     /translation="MPERPVGPADPAAALADLRSARRRKRVADIHWIDALYQVYLAAL
                     VSLAAVLLLSSWIGGERLGPLRTAQVLHDGPDIVGVGVALAILIGLRSGARGGPLAIE
                     PADARHVLLAPIDRALALRSPALHQLRFAAFVAVVVGAIGGQLAVRRLGDEPVLWILS
                     GAAFALTTLGLAYGAALIAGGRRLSIRVANIVGVALLAWAVADAVDAQHIDIHYSPTG
                     LVGTIPFWPLEFEPLALIPAVLAIVFVLLGVIGLGGLSLEAAERRTRLVGQLKFAATL
                     QDLRTVMVLRRQLAMELPRTKPWIRERGSGRLPVWHRGMRGVLRWPAARVVRLIGFGV
                     VAGICLRGVANGTIPLVIVAGLALYLAALDGVESMAQEADHPGRNEAIPRPIGDL"
     gene            complement(1222..1890)
                     /locus_tag="VFP02_10470"
     CDS             complement(1222..1890)
                     /locus_tag="VFP02_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET9543498.1"
                     /translation="MAYVDLERDGAVFVLRMVDDDNRFHADSVAEWHAAVDEVEASTG
                     PAALVTTGTGKFYSNGFALEQIIEDGTIGAVIPEALRLFARFLRLPVYTVAALNGHAF
                     AAGAMLAMTHDARVMRADRGFWCLPEADMGVPLAPGMTALIAARLPIATAHRAIMTAH
                     RWGGEDAVTAGIVDEAVAEDQVLARAVELATARADTSREATTALRVGLYGDLIAVLEA
                     GRIS"
     gene            complement(1895..3433)
                     /locus_tag="VFP02_10475"
     CDS             complement(1895..3433)
                     /locus_tag="VFP02_10475"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET9543499.1"
                     /translation="MIAASLALLAQLDVPEPVETPDIAWSALLPLVCLTVGAVLLVLI
                     TSLLKGRLFKGFDAAFTVLTAAAGIGASIYLWQRIQDEGPSSTAAGAVAVDGFGVFLT
                     VVICVSVILAALISDRYLRREELDGPEYFVLMLLSAAGGVTMAIANDLIVVFLGLETL
                     SIAAYVLAAMQRHRTQSQEAGLKYFVLGAFSSAFFLYGIAFVYGATGSTNLSRINAFL
                     ADNLLLSDGVLLIGMVLLLVGLGFKVAAVPFHQWSPDVYQGSPSPAVSYMAGAVKAAA
                     FAAMVRIFVTTFDQSAVDWQPVVYALAVLSLIVGAVLAAVQTDVKRMMAYSSINHAGF
                     ILIGVEAASAAGTAAVLFYVAAYAVIVAGTFGVITVVAGSRDAKTSVEDFGGLSQRRP
                     VLALGFTVLLVAQAGVPFTSGFFAKFTVVLAAEGAQSYWLAIIAMVTAVIAAFVYLRV
                     VVAMYMRDPAEDGEEREKEAVPWTAALGIGLAVAITLVLGILPGILTDIADRAVPVLV
                     AVTP"
     gene            complement(3430..>4339)
                     /locus_tag="VFP02_10480"
     CDS             complement(3430..>4339)
                     /locus_tag="VFP02_10480"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET9543500.1"
                     /translation="FALAFSVKVPLFPLHTWLPDAHTEAPTAGSVILAGVMLKLGTYG
                     FLRFGVYLFPEASVYFAPALVTLGVIGILYAAVVATMQKDLKRLVAYSSVAHLGFIVL
                     GAFAFTTQGLTGSVLQMVNHGLSTGALFLLVGMIYERRHTRLIADLGGLQKPAPVMAF
                     VFTVVMLSSIGLPGLNGFVGEFLILVGSFLTREWWAVVAATGVIVAALYLLWAYQRVF
                     HGPAEGENAEMADINKWELACMAPLLAGIVFLGVYPKPVLERIEPAVNELIAHLEEFS
                     DYHEPDVAVDGPAAEVPVETEGEVHE"
BASE COUNT          747 a         1427 c         1667 g          498 t
ORIGIN      
        1 aggtcgccga tcgggcgagg gatggcctcg ttgcgtccgg ggtgatcggc ctcctgggcc
       61 atcgactcca cgccgtcgag ggcggccagg tagagggcga ggccggcgac gatgacgagg
      121 gggatggtgc cgttggccac accccgcagg cagatgccgg ccacgacccc gaagccgatg
      181 aggcgcacga cccgagccgc cggccagcgc aggacgcccc gcatgccgcg gtgccagacc
      241 gggaggcggc cggagccacg ctcgcggatc cacggcttgg tccggggcag ctccatggcc
      301 agctggcggc gcaggaccat gaccgtgcgc aggtcctgga gggtggcggc gaacttgagc
      361 tggccgacca gtcgggtccg gcgctccgcc gcctcgaggg agaggccgcc gagaccgatg
      421 acaccgagga ggacgaacac gatcgccagg accgccggga tcagggccaa tggctcgaac
      481 tcgaggggcc agaacgggat cgtgccgacc aggcccgtcg gtgagtagtg gatgtcgatg
      541 tgctgcgcgt cgacggcgtc ggccaccgcc caggccagga gggcgacacc gacgatgttg
      601 gccacccgga tcgagagtcg tcgacccccg gcgatgaggg cggcgccgta ggccaggccc
      661 agggtggtca gggcgaacgc ggcaccgctg aggatccaga gcacgggctc gtcccccagg
      721 cgacgcacgg cgagctggcc cccgatggcg cccaccacga cggccacgaa ggcggcgaag
      781 cgcagctggt gcagggcggg gctgcgcagg gcgagggccc ggtcgatggg ggccaggagg
      841 acgtgacggg cgtcggccgg ctcgatggcc agcggtccac cgcgggcccc ggagcgcagg
      901 ccgatgagga tggccagggc cacgcccaca ccgacgatgt cgggaccgtc atggaggacc
      961 tgcgccgttc gcaacggtcc gaggcgctcg ccgccgatcc agctggagag caggagcacg
     1021 gcggccaggg acaccagggc ggccaggtac acctggtaga gggcgtcgat ccagtggatg
     1081 tcggccaccc gcttgcgccg tcgggccgac cgcagatcgg ccagcgccgc ggcggggtcg
     1141 gcgggaccga cggggcgctc aggcacggct ggtcagcgta cgaccggccg cgccccgtga
     1201 ggtggtcgcg gcgcctcgta ctcagctgat ccggccggct tccagcacgg cgatgaggtc
     1261 accgtagagg ccgacgcgca gggcggtggt ggcctctcga gaggtgtcgg cccgggcggt
     1321 ggccagctcg acggcgcggg ccaggacctg gtcctcggcc acggcctcgt cgacgatgcc
     1381 cgcggtcacg gcatcctcgc cgccccagcg gtgggcggtc atgatcgccc ggtgggccgt
     1441 ggcgatcggc agcctggccg cgatgagcgc ggtcatgccc ggggcgaggg ggacgcccat
     1501 gtcggcctcg ggcaggcacc agaacccgcg gtcggcgcgc atgacccggg cgtcgtgggt
     1561 catggccagc atcgcccccg cggcgaaggc gtggccgttc agggcggcca cggtgtagac
     1621 gggcaggcgc aggaagcggg cgaagagacg cagggcctcg gggatcaccg cgccgatcgt
     1681 gccgtcctcg atgatctgct cgagggcgaa gccgttcgaa tagaacttgc ccgtcccggt
     1741 ggtgacgagg gcggcggggc cggtcgaggc ctcgacctcg tcgacggcgg cgtgccactc
     1801 ggccaccgag tcggcgtgga accggttgtc gtcgtccacc atgcggagga cgaacaccgc
     1861 cccgtcccgc tccagatcca cgtaggccac gggatcaggg ggtgacggcg accagcacgg
     1921 gaacggcccg gtcggcgatg tcggtcagga tcccgggcag gatccccagc accagcgtga
     1981 tggcgaccgc caggccgatg ccgagggcgg cggtccacgg caccgcctcc ttctcgcgct
     2041 cctcgccgtc ctccgccggg tcccgcatgt acatggccac gaccacccgc aggtagacga
     2101 aggccgcgat cacggcggtg accatggcga tgatggccag ccagtaggac tgggccccct
     2161 cggcggccag gacgacggtg aacttggcga agaagccgga ggtgaacggc acgccggcct
     2221 gggccaccag cagcaccgtg aagccgaggg cgaggacggg acggcgctgg gacaggccac
     2281 cgaagtcctc gaccgaggtc ttggcgtccc gggagccggc caccacggtg atgaccccga
     2341 aggtcccggc cacgatgacg gcgtaggcgg cgacgtagaa cagcacggcc gcggtcccgg
     2401 ccgccgaggc ggcctcgacc ccgatgagga tgaacccggc gtggttgatc gacgagtacg
     2461 ccatcatccg cttgacgtcg gtctggacgg cggcgagcac ggccccgacg atcaggctga
     2521 gcacggccag ggcatagacg acgggctgcc agtcgaccgc cgactggtcg aaggtggtga
     2581 cgaagatgcg caccatggcg gcgaaggcgg cggccttcac ggccccggcc atgtaggaca
     2641 ccgccggcga cggcgagcct tggtagacat cggggctcca ctggtggaac ggcaccgccg
     2701 ccaccttgaa gccgaggccg accaggagca ggaccatgcc gatgagcagc acgccgtcgg
     2761 acagcagcag gttgtcggcc aggaaggcgt tgatccgcga gaggttggtc gagccggtgg
     2821 cgccgtagac gaaggcgatg ccgtagagga agaaggccga cgagaaggca ccgaggacga
     2881 agtacttgag gccggcctcc tgggactggg tccggtggcg ctgcatggcg gcgaggacgt
     2941 aggccgcgat cgacagcgtc tcgaggccga ggaagaccac gatcaggtcg ttggcgatgg
     3001 ccatcgtgac cccgccggcc gccgacagga gcatgaggac gaagtactcg ggaccgtcca
     3061 gttcctcgcg gcgcaggtac cggtcgctga tcagggcggc caggatcacc gagacgcaga
     3121 tgaccacggt caggaagacg ccgaagccgt cgacggccac cgccccggcc gcggtcgacg
     3181 aggggccctc gtcctggatg cgttgccaga ggtagatcga ggcgccgatg ccggccgcgg
     3241 cggtgagcac ggtgaaggcg gcgtcgaagc ccttgaacag gcgacccttg agcagtgacg
     3301 tgatcaggac cagcagcacc gcgcccacgg tcaggcagac cagggggagc agggccgacc
     3361 aggcgatgtc cggggtctcc accggctcgg gcacgtcgag ctgggccagg agggcgagcg
     3421 aggcggcgat cattcgtgca cctcaccctc ggtctcgacc gggacctcgg cggcggggcc
     3481 atcgacggcc acgtccggct cgtgatagtc gctgaactcc tcgaggtggg cgatgagctc
     3541 gttcaccgcg ggctcgatgc gctccaggac cggcttgggg tagacgccca ggaacacgat
     3601 gccggccagc agcggggcca tgcaggccag ctcccacttg ttgatgtcgg ccatctcggc
     3661 gttctcaccc tcggccgggc cgtggaacac ccgctggtag gcccagagca ggtagagggc
     3721 ggccacgatg actccggtcg cggcgaccac ggcccaccac tcgcgggtca ggaacgagcc
     3781 gacgaggatc aggaactcgc ccacgaagcc gttgaggccg ggcaggccga tggaggacag
     3841 catcaccacg gtgaagacga aggccatgac cggcgccggc ttctgcaggc cgcccaagtc
     3901 ggcgatcagg cgcgtgtggc gacgctcgta gatcatcccc accagcagga agagggcgcc
     3961 ggtggacagg ccgtggttga ccatctgcag gaccgagccg gtcaggccct gggtcgtgaa
     4021 ggcgaaggcg ccgagcacga tgaacccgag gtgggccacc gacgagtagg ccaccagccg
     4081 cttcaggtcc ttctgcatcg tggccacgac ggcggcgtag aggatgccga tgacgccgag
     4141 ggtgaccagc gcgggagcga agtagaccga ggcctcgggg aacaggtaca cgccgaagcg
     4201 caggaagccg taggtgccca gcttcagcat gaccccggcc aggatcaccg agcccgcggt
     4261 gggggcctcg gtgtgggcgt ccggcagcca ggtgtgcagc gggaacagcg ggaccttgac
     4321 cgagaaggcc agcgcgaag
//
LOCUS       DASTFV010000573         4910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2107514, whole genome shotgun sequence.
ACCESSION   DASTFV010000573 DASTFV010000000
VERSION     DASTFV010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2107514"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFP02_10485"
     CDS             complement(<1..306)
                     /locus_tag="VFP02_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543501.1"
                     /translation="MTLPLFVLAAVCAVNAPRCRTVARHWRLAGLGAGLTFVVLLPLA
                     VLADPLLDAVGISAATARLATGLLLLATGLLVFGWPRPEPEPALPGWRAAVVPIAFPT
                     "
     gene            complement(303..1454)
                     /locus_tag="VFP02_10490"
     CDS             complement(303..1454)
                     /locus_tag="VFP02_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543502.1"
                     /translation="MGTTVRARVGAGLLAWLGGLAVVVAAAHPESCPTVTADEARAAA
                     QAGVDWIGTNQALDGRFLYRYDRAEHEAQPGYNYVRHAGTLLALEQGAAAGLEGAAET
                     AAAGFEFVDDHLTDLPDGRTAFTADTGGAGLLVAALVEHRRRTGDESNDELLVHLGRF
                     LESTVTDEGAIVALWDLEADEPVAGTRSPFFTGEILWALARLHAEFPDEGWDVPARRV
                     STYLATERDDAERRLPPVSDHWGAYAWDEIAAWPTPLTDDELAYAERQAGLFGLQVRY
                     ESQRRESGLARLTRGPHALPAGLGTVGEGLGGIRRLAEREPRLDVDREDLDDRLTCVA
                     GMLADRQTHSDDPRLDGAWFRADDVTQVDDQQHAVSALLAALPVLEDHP"
     gene            1492..1911
                     /locus_tag="VFP02_10495"
     CDS             1492..1911
                     /locus_tag="VFP02_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543503.1"
                     /translation="MTNRGRWVVTVAVGLVVVAPAVLPHPADDFPISTYPMFTTERGE
                     VVDIDTAVRVDEAGRHRLSPQIVGGTSEIVSAAVTVSRAIAGGGAALTRLCGEIADRV
                     DEPGSIEIVTERHDAVALLQDGAEPLEVTVHERCDGP"
     gene            1896..2786
                     /locus_tag="VFP02_10500"
     CDS             1896..2786
                     /locus_tag="VFP02_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543504.1"
                     /translation="MRRAVNQVAPARRLAAFRILTGGFALVFLVSRFLYFVDLTRIHE
                     SRWLPPLALRPLGDPPSQAVALVILGLTLALGVAYAAGWRFRATGPAFAVGLLAVMSY
                     RNAWGHVFHTDNLLVLHVFVVGLAPSADAWALDARARPEPVEDVRYGFPLRLAALITV
                     LTYVVTGVAKLRYAGGDWLAGDTLLHQIAFDNARKNVLGDDYSPLAAAFASHPWLFRP
                     MGLLTLVVELGAPLALLGRRWAAWWSGAAWLFHVGIFGLMWIAFPYPLSLIAFAPFFS
                     IERPVDWLLDRSTVRRLAPS"
     gene            2783..3532
                     /locus_tag="VFP02_10505"
     CDS             2783..3532
                     /locus_tag="VFP02_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HET9543505.1"
                     /translation="MSAVEDARRLVQAAEAVAVLTGAGISTDSGIPDFRGPNGIWTKN
                     PAAEKSATLQNYLADPEVRKQAWRNRLDSPAWTAQPNAGHRALVALERQGKLHTLITQ
                     NIDGLHQAAGSDPDLVVEVHGTIREVGCMNCGERAPMERALDRVRAGEADPECRTCGG
                     ILKSATISFGQSLVADDLARAERAARECDLLLAVGSTLAVYPICDVVPIARRLGASVI
                     IVNAEPTQFDDLADAVVRGSISEVLPEIIGK"
     gene            3615..4784
                     /gene="moeB"
                     /locus_tag="VFP02_10510"
     CDS             3615..4784
                     /gene="moeB"
                     /locus_tag="VFP02_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HET9543506.1"
                     /translation="MPNFRELLSQTKARIREISTAEAEAARDADGAVVLDVREPDEYE
                     QGAIPGSVHIARGNLESQVEGRLLDKDAPVVVFCAGGTRSAFAAETLGQLGYADVASV
                     AGGFNKWKDEGRDWKVPSVLSPEQRNRYQRHLLLPEVGVEGQQKLLGARVLLLGAGGL
                     GSPAALYLAAAGVGTLGIIDMDVVDESNLQRQILHNMDRIGERKVDSAKKTLTAINPD
                     VDVVTYDMRLDAGNVVALLSQFDVVIDGADNFPSRYLLNDASLKTDTPVVHGSIFRFE
                     GQATVFKPFDGPCYRCMIPEPPPAELAPSCAEAGVLGVLPGIIGSIQALEAIKLILGI
                     GDDLSGRLLAYDSLEQSFRTFKVNRDPECPACSLDPADIVIAEYDEFCMPHPVGA"
BASE COUNT          678 a         1818 c         1714 g          700 t
ORIGIN      
        1 cgtcggaaag gcgatcggga ccacggcggc ccgccacccc gggagggccg gttcgggctc
       61 ggggcgcggc cagccgaaga ccagcagccc ggtcgcgagc aggagcaggc ccgtggccag
      121 ccgggccgtc gccgcactga tgccgacggc gtccagcagg ggatcggcga gcacggcgag
      181 ggggagcagc acgacgaagg tcaggcccgc accgaggccg gcgagtctcc agtgccgtgc
      241 caccgtgcgg caccgcgggg cgttcacggc gcacacggcg gccaggacga acagggggag
      301 ggtcatgggt ggtcctccag gaccgggagg gcggcgagca gggcggacac ggcgtgctgc
      361 tggtcgtcga cctgggtgac gtcgtcggct cggaaccagg cgccgtcgag gcggggatcg
      421 tcgctgtggg tctggcggtc ggccagcatc ccggcgacgc aggtcaggcg atcgtccagg
      481 tcctcccggt cgacgtcgag gcggggctcg cgctcggcga ggcgacggat cccgcccagg
      541 ccctcgccca ccgtgccgag cccggcgggc agcgcgtggg gcccgcgggt gaggcgggcc
      601 aggcccgatt cccggcgctg cgactcatag cggacctgca ggccgaacag gccggcctgg
      661 cgctcggcgt aggccaactc gtcgtcggtc agcggggtgg gccaagccgc gatctcgtcc
      721 caggcgtagg cgccccagtg gtccgagacg ggcggaagac gacgctcggc gtcgtctcgc
      781 tcggtggcca ggtaggtcga cacccgtcgg gcggggacgt cccacccttc atcggggaac
      841 tcggcatgga ggcgggccaa cgcccagagg atctcgccgg tgaagaacgg ggagcgggta
      901 ccggccaccg gctcatcggc ctccaggtcc cagagggcga cgatcgcgcc ttcgtcggtg
      961 accgtgctct ccaggaaacg gccgaggtgg accaacagct cgtcattcga ctcgtcgccc
     1021 gttcggcggc ggtgctcgac gagggcggcc acgagcagcc ctgcgccgcc ggtgtccgcg
     1081 gtgaaggccg tccgtccgtc cgggagatcg gtgaggtggt cgtcgacgaa ctcgaagccg
     1141 gcggcggcgg tctcggccgc cccctcgagg ccggccgccg cgccctgctc gagggccagc
     1201 agcgttccgg cgtgacggac gtagttgtac ccgggctggg cctcgtgctc ggcccggtcg
     1261 tagcggtaga ggaaacgccc gtcgagcgcc tggttggtgc cgatccagtc cacgccggcc
     1321 tgcgccgcgg cccgtgcctc gtcggccgtc accgtggggc acgactccgg gtgcgcggcc
     1381 gcgaccacca ccgccagccc accgagccag gccagcagcc ccgcgcccac ccgtgcccgc
     1441 accgtcgtcc ccaccaaccg ggacgctact gagccgtacg ctcggcgggt cgtgacgaac
     1501 cgggggcggt gggtggtgac ggtggcggtg gggctggtcg tggtcgcccc ggccgtcctc
     1561 ccgcatccgg ccgacgactt cccgatctcg acctatccga tgttcacgac cgagcggggc
     1621 gaggtcgtgg acatcgacac cgcggtgcgg gtcgacgagg ccggtcggca ccggttgtcg
     1681 ccgcagatcg tgggcgggac gagcgagatc gtcagcgccg ccgtcaccgt cagccgagcc
     1741 atcgcggggg gcggcgcggc gcttacccgg ctgtgcggcg agatcgccga ccgagtggac
     1801 gagccgggat cgatcgagat cgtgaccgag cgccacgacg ccgtggccct cctccaggac
     1861 ggggcggagc ccctcgaggt gaccgtgcac gagcggtgcg acgggccgtg aaccaggtcg
     1921 ccccggcccg gcgcctggcc gcgttccgga tcctgaccgg cgggttcgcg ctggtcttcc
     1981 tggtcagccg cttcctctac ttcgtcgacc tgacccggat ccacgagtcc cgctggctcc
     2041 cgcccctggc cctccgtcct ctgggcgatc caccgtccca ggcggtcgcc ctcgtcatcc
     2101 tcggcctgac cctggccctc ggcgtcgcct acgccgcggg ctggcggttc cgagcgacag
     2161 gtccggcgtt cgcggtgggc ctgctcgccg tcatgagcta ccgcaacgcg tggggccacg
     2221 tgttccacac cgacaacctc ctggtgctcc acgtgttcgt cgtggggctg gccccgtcgg
     2281 ccgacgcctg ggccctcgac gcccgggccc gccccgagcc ggtcgaggat gtccgctacg
     2341 gcttcccgct ccgcctcgcc gcgctgatca ccgtgctcac ctatgtcgtc accggcgtgg
     2401 ccaagctccg ttacgccggc ggcgactggc tggcgggcga caccctcttg caccagatcg
     2461 ccttcgacaa tgcccgcaag aacgtgctgg gcgacgacta ctcgcccctc gcggcggcct
     2521 tcgccagtca cccctggctg ttccggccga tgggcctgct cacgctggtc gtcgagctcg
     2581 gggcgccgct ggctctgctc gggcgccggt gggcggcgtg gtggtcgggg gcggcgtggc
     2641 tgttccacgt cgggatcttc ggcctcatgt ggatcgcctt cccgtacccg ctcagcctca
     2701 tcgccttcgc cccgttcttc agcatcgagc gtccggtcga ctggctcctc gatcgatcga
     2761 cggtccgtag gctggcgccg tcatgagtgc cgtggaggac gcccgccgcc tggtccaggc
     2821 cgccgaagcg gtcgcggtgc tcaccggcgc cggcatctcc accgacagtg gcatccccga
     2881 cttccgcggc cccaacggca tctggaccaa gaaccccgcc gccgagaagt cggccaccct
     2941 gcagaactac ctggccgacc ccgaggtcag gaagcaggcg tggcgcaacc gcctcgactc
     3001 gcccgcttgg acggcccagc ccaacgctgg tcaccgggcg ctcgtcgccc tggagcgtca
     3061 ggggaagctg cacaccctga tcacccagaa catcgacggc ctccaccagg ccgccggcag
     3121 cgatcccgac ctcgtcgtcg aggtccacgg caccatccgc gaggtgggct gcatgaactg
     3181 cggtgagcgg gcgccgatgg aacgggcctt ggaccgggtc cgggcgggcg aagccgaccc
     3241 cgagtgccgg acctgcggcg ggatcctcaa gtcggccacc atctcgttcg gccagagcct
     3301 ggtggccgac gacctggcgc gggccgaacg ggccgcccgg gagtgtgacc tcctgctcgc
     3361 cgtcggctcg acgctggccg tctaccccat ctgcgacgtc gtcccgatcg cccgacgcct
     3421 cggggcgtcg gtgatcatcg tcaacgccga gcccacccag ttcgacgacc tggccgacgc
     3481 cgtcgtccgc ggctccatca gcgaggtgct ccccgagatc atcgggaaat aggcggcccc
     3541 gatcgaatgt tgaccgaatc gctaggcttt tcctggcccc cgaacgacga agtgacgacg
     3601 aggtcgatgt cccgatgccc aacttccgcg agctgctgag ccagaccaag gcccgcatcc
     3661 gagagatctc cacggcggag gccgaagccg cccgcgacgc cgacggggcg gtcgtcctcg
     3721 acgtccgcga gcccgacgag tacgagcagg gcgccatccc gggttcggtg cacatcgccc
     3781 gaggcaacct cgagagccag gtcgaagggc gactgttgga caaggacgca ccggttgtcg
     3841 tcttctgcgc cggtggcacc cgttccgcct tcgccgccga gacgttgggc cagctcggct
     3901 acgcggacgt ggcgtcggtg gccggagggt tcaacaagtg gaaggacgag ggccgcgact
     3961 ggaaggtccc gtcggtgctc tcgccggagc agcgcaaccg ctaccagcgt cacctcctcc
     4021 tgcccgaggt cggcgtcgag ggccagcaga agctgctcgg cgcccgggtg ctcctcctcg
     4081 gcgccggtgg cctcggctcg cccgccgcgc tctacctcgc cgccgcgggg gtggggaccc
     4141 tcggcatcat cgacatggac gtggtcgacg agtccaacct gcagcgccag atcctgcaca
     4201 acatggaccg catcggtgag cgcaaggtcg actcggccaa gaagaccctg accgccatca
     4261 accccgacgt ggacgtcgtc acctacgaca tgcgcctcga tgccggcaac gtcgtcgccc
     4321 tcctgtccca gttcgacgtg gtcatcgacg gggccgacaa cttccccagc cgctacctgc
     4381 tcaacgacgc ctcgctgaag accgacacgc cggtcgtgca cggctcgatc ttccgcttcg
     4441 agggccaggc cacggtgttc aagcccttcg acgggccctg ttaccgctgc atgatccccg
     4501 agcctccgcc ggccgagttg gccccctcct gcgccgaggc cggcgtgctg ggcgtcctgc
     4561 ccggcatcat cggctcgatc caggcgctcg aggccatcaa gctcatcctc ggcatcggcg
     4621 acgacctgtc cggccgcctg ctggcctacg actccctcga gcagtccttc cgcacgttca
     4681 aggtcaaccg tgatcccgag tgcccggcct gctccctcga cccggccgac atcgtcatcg
     4741 ccgagtacga cgagttctgc atgccccatc cggtcggcgc ctgacggccg gtcgccccgc
     4801 gaggaccgcg tcgcggcgcg ggggcggccc taacctgacg cggcgatgct cgtcggcgaa
     4861 cgcgtcatct acctccagct ccagaagacg ggcagcaccc ggatcaccga
//
LOCUS       DASTFV010000574         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2118024, whole genome shotgun sequence.
ACCESSION   DASTFV010000574 DASTFV010000000
VERSION     DASTFV010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2118024"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFP02_10515"
     CDS             <1..187
                     /locus_tag="VFP02_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="flap endonuclease"
                     /protein_id="HET9543507.1"
                     /translation="RENLEDALLFRRIATLELDSITTSVEELEWTGPTKALVAMSERL
                     DAPGLLERAVKLAEQRA"
     gene            complement(225..1298)
                     /locus_tag="VFP02_10520"
     CDS             complement(225..1298)
                     /locus_tag="VFP02_10520"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET9543508.1"
                     /translation="MDLPVMPPVRPMLAKAVHAMPDTEGLLFEPKWDGFRCIVFRDGD
                     EVELGSRNDRPLTRYFPELPPVLRDALPEKCVVDGEVVVPIGAKLDFEALQQRIHPAD
                     SRVNMLAEKTPASFVAFDLLAVGDEDLRELPFRERRKRLEKILGKVKPPLHLTPNTED
                     RAVAEDWFDRFEGAGFDGVMAKDPEDPYLENKRSQLKVKHKRTADCVVAGYRTHKDGE
                     GIGSLLMGVYDDEGELHHLGVAASFSAAKRKELIAELAPYEKDALADHPWREWSDVMA
                     HEEGGRKPGAQNRWTGSKDQSWSPLRLELVAEVTYDGLQSGRFRHTSHWVRWRPDKTP
                     AECTFDQFSITAPAELQQVFADD"
     gene            1336..>2092
                     /locus_tag="VFP02_10525"
     CDS             1336..>2092
                     /locus_tag="VFP02_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET9543509.1"
                     /translation="MATTPDPLEARSPTAAYSMRLRVLLDNVPGTLGRLTTTIGAAGA
                     NIRGLEGFEVKGAVLDDEVVVDCVDEAHQHEVLDAIKQLEGVELVEWEDRTFALHDGG
                     KIEVLSLGKVGDRDDLSMAYTPGVARVCLAIERDPARVHDLTIKRNTVAIVTDGTAVL
                     GLGDIGPEASLPVMEGKALLFKEFAGVDAFPICLDVNSTEEIIETVERIAPVFGGINL
                     EDIAAPACFDVEERLRESLDIPVFHDDQHGTAVV"
BASE COUNT          315 a          699 c          743 g          335 t
ORIGIN      
        1 ccgggagaac ctggaggacg ccctcctgtt ccgccgcatc gccaccctcg agctcgactc
       61 gatcacgacg tccgtcgaag agctcgagtg gaccggcccg acgaaggccc tggtcgccat
      121 gagtgagcgc ctcgacgccc ctggcctcct cgaacgcgct gtgaaactcg ccgagcagcg
      181 cgcctgaccg gcgtcagccg ggcgcaacac ggggggtccc gggatcagtc gtcggcgaag
      241 acctgctgga gttcggcggg tgcggtgatg gagaactggt cgaaggtgca ctcggctggg
      301 gtcttgtcgg ggcgccagcg gacccagtgc gacgtgtggc ggaagcggcc ggactgcagg
      361 ccgtcgtagg tcacctcggc caccagctcc aggcgcagcg gggaccagga ctggtccttg
      421 gagccggtcc accggttctg ggctccgggc ttgcgccccc cttcctcgtg ggccatcacg
      481 tcggaccact cacgccaggg gtggtcggcg agcgcgtcct tctcgtacgg ggccagctcg
      541 gcgatcagct ccttgcgttt tgcggcggag aaggaggcgg cgacgcccag gtggtgcagc
      601 tcgccctcgt cgtcgtagac gcccatcagg agcgagccga tgccctcgcc gtccttgtgg
      661 gtccggtagc cggcgaccac gcagtcggcg gtgcgcttgt gcttgacctt gagctggctg
      721 cgcttgttct cgaggtaggg gtcctcgggg tccttggcca tgaccccgtc gaagccggcg
      781 ccctcgaagc ggtcgaacca gtcctcggcc acggcccggt cttcggtgtt gggcgtgagg
      841 tggagcggtg gcttgacctt tccgaggatc ttctcgaggc gctttcggcg ctcgcggaag
      901 ggcagctcgc gaaggtcctc gtcgcccacc gccagcaggt cgaaggccac gaagctggcg
      961 ggggtcttct cggccagcat gttcaccctg gagtcggcgg ggtggatgcg ctgctggagc
     1021 gcctcgaagt cgagcttggc cccgatgggg accacgacct cgccgtcgac cacgcacttc
     1081 tcgggcaaag cgtcccgcag gacgggaggc agctcgggga agtaccgggt cagcgggcgg
     1141 tcgttgcggc tgcccagctc cacctcgtca ccgtcccgga agacgatgca gcggaagccg
     1201 tcccacttcg gctcgaacag caggccttcg gtgtcgggca tggcatgtac ggccttggcc
     1261 agcatcggcc ggaccggcgg catcacgggc agatccatgc agagggaggt tagggaactc
     1321 ggtaggttcc cgggaatggc aacgacgccc gatccgctgg aggcccgctc gccgacggcg
     1381 gcctactcga tgcgcctgcg ggtgctgctg gacaacgtgc ccggcaccct cggtcgcctc
     1441 accaccacca ttggcgcggc cggcgccaac atccgggggc tcgagggctt cgaggtgaag
     1501 ggcgcggtgc tggacgacga ggtggtcgtc gactgtgtcg acgaggccca ccagcacgag
     1561 gtcctcgacg ccataaagca gctggagggc gtcgagctgg tcgagtggga ggaccgcacc
     1621 ttcgcccttc acgacggcgg caagatcgag gtcctgtcgc tgggcaaggt cggcgaccgt
     1681 gacgacctgt cgatggcgta cacgccgggt gtggcccggg tctgcctggc catcgaacgg
     1741 gatccggccc gggtccacga cctgaccatc aagcgcaaca ccgtggccat cgtcaccgac
     1801 gggaccgcgg tcctcgggct gggcgacatc ggccccgagg cctccctccc cgtcatggag
     1861 ggcaaagcgc tgctcttcaa ggagttcgcc ggcgtcgacg cctttccgat ctgtctcgac
     1921 gtcaacagca ccgaggagat catcgagacg gtcgagcgca tagctccggt cttcggcggc
     1981 atcaacctcg aggacatcgc cgcgcccgcc tgcttcgacg tcgaggagcg cctgcgggag
     2041 agcctcgaca tccccgtgtt ccacgacgat cagcacggca ctgcggtggt ga
//
LOCUS       DASTFV010000575         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2121293, whole genome shotgun sequence.
ACCESSION   DASTFV010000575 DASTFV010000000
VERSION     DASTFV010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2121293"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFP02_10530"
     CDS             <1..210
                     /locus_tag="VFP02_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET9543510.1"
                     /translation="HHAVVRGVERLSGAPVRAPDIRAGAALALAGLAAEGETVVADAD
                     HIDRGYEDFVGKLTAVGADLERRDN"
     gene            complement(207..368)
                     /locus_tag="VFP02_10535"
     CDS             complement(207..368)
                     /locus_tag="VFP02_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543511.1"
                     /translation="MVTLAHQGGWDEMAMVAVPLLLVTILLVVANRRASAELARQRGG
                     GDTADPDPV"
     gene            440..736
                     /locus_tag="VFP02_10540"
     CDS             440..736
                     /locus_tag="VFP02_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET9543512.1"
                     /translation="MTSQEQIAVPESDHPLDEKVRVTIEAIRPAVQADGGDIMFHGID
                     DDGVVTVELVGACVSCPASTLTLKAGIERILRDRVEGVTAVRDVSEDTETLLSL"
     gene            743..1675
                     /gene="meaB"
                     /locus_tag="VFP02_10545"
     CDS             743..1675
                     /gene="meaB"
                     /locus_tag="VFP02_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET9543513.1"
                     /translation="MDTEALLDRARDGDRTALARLLSLVERGGEPGRAIGRLAHRSPG
                     SAHTIGITGPPGAGKSTLTSALVQVMRAAGHRVAVLAVDPSSPFTGGAILGDRVRMGE
                     HALDNEVFIRSMASRGHLGGLSLAAPEAVRVLDAVGFTFVIVETVGVGQVEVEVAAET
                     DTTVVVINPGWGDAVQANKAGLLEVADIFCVNKADRPGAAEAGRDLGQMLDMTGARDW
                     RPPVIDTVATDGTGVADLWAAILAHQAAQTASGSLAAERADRVRVELSRVLARRLLEQ
                     AEARTQGEGFDAVCAQVVAGEIDPWEAADRLLAD"
     gene            1685..2503
                     /locus_tag="VFP02_10550"
     CDS             1685..2503
                     /locus_tag="VFP02_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9543514.1"
                     /translation="MTEDSPELIHVERGDDGVAVITLDNGKVNALSIALLDQLRAAAE
                     DLTADPPGAVIITGGARIFAAGAQITEFLDDPDALPSQVADQNRLTAQGHAFLIALNA
                     VAAIPRATIAEVGGFALGGGCELALACDFRIASDRAKLGQPEILLGIIPGGGGTQRLS
                     RLVGPAKAKDLVLTGRQVGAAEALAMGLVDEVVSAEELAAHVRAKAAALAGGAVLAQA
                     LAKRAIDEGLDRGLAEGLALEQELFIASFATDDAGIGINSFLEHGPGEADFTGR"
BASE COUNT          364 a          949 c          966 g          350 t
ORIGIN      
        1 catcacgccg tggtccgggg ggtcgagcga ctgtccgggg cccccgtgcg ggcccccgac
       61 atccgggcgg gcgccgccct ggccctggcc ggtctggccg ccgagggcga gaccgtcgtg
      121 gccgacgccg accacatcga ccggggctac gaggacttcg tcggcaagct caccgccgtc
      181 ggcgcggacc tggaaagaag ggacaactag acagggtcgg ggtcggcggt gtccccgcca
      241 ccacgttggc gggccagttc ggccgaggcg cggcggttgg ccaccacgag caggatcgtc
      301 accaggagga gcggtacggc caccatggcc atctcgtccc agccgccctg atgggccagt
      361 gtgaccacga gcacacgcta ggccgggaag gcccccgccc ggtacgcggt tggacgaggg
      421 gcgcacccct acactcgccg tgacgtccca ggagcagatc gctgtgcccg agtccgacca
      481 cccgctggat gagaaggtcc gtgtgaccat cgaggccatc cgtccggctg tccaggctga
      541 cgggggcgac atcatgttcc acggcatcga cgacgacggc gtcgtcaccg tcgagctggt
      601 cggggcctgt gtctcctgcc ccgcatcgac gctgaccctg aaggcgggca tcgagcggat
      661 cctccgggac cgggtcgagg gcgtgacggc cgtccgggac gtctccgagg acaccgagac
      721 cctcctcagc ctgtagccac ccgtggacac cgaggcgctg ttggaccgcg ctcgggacgg
      781 cgaccgcacg gcgttggccc gcctgctgtc gctcgtggag cggggcggcg agcccggccg
      841 ggcgatcggc cgcctggcgc accggtcgcc ggggagcgcc cacacgatcg gcatcaccgg
      901 accgcccggg gccggcaagt cgaccctgac gtccgcgctc gtccaggtga tgcgggcggc
      961 tgggcaccgc gtggccgtac tggccgtgga cccctcctcg cccttcaccg gtggcgccat
     1021 cctgggtgac cgcgtgcgca tgggcgagca cgccctcgac aacgaggtct tcatccgatc
     1081 catggcttcc cggggccatc tcggcggcct gtcgctggcg gcccccgagg ccgtgcgggt
     1141 gctcgacgcc gtgggcttca cgttcgtgat cgtggagacc gtcggtgtgg gccaggtcga
     1201 ggtcgaggtg gcggccgaga ccgacaccac cgtggtggtg atcaaccccg gctgggggga
     1261 tgccgtgcag gccaacaagg ccgggctgct cgaggtggcc gacatcttct gcgtgaacaa
     1321 ggccgaccga cccggcgcgg ccgaggccgg tcgggacctc gggcagatgc tggacatgac
     1381 cggggcccgg gactggcgtc cgccggtgat cgacaccgtg gccaccgacg gcacgggcgt
     1441 ggccgacctc tgggcggcga tcctggccca tcaggcggcg cagaccgcca gcgggtccct
     1501 ggccgcggag cgggcggacc gcgttcgggt cgaactgtcc cgcgtcctgg cccgccgcct
     1561 gttggagcag gccgaggccc gaacgcaggg cgaggggttc gacgcggtct gcgcccaggt
     1621 cgtcgccggc gagatcgacc cctgggaagc agccgatcgc ctcctcgctg actagcttcc
     1681 ggccatgacc gaagactcgc cggagctgat ccacgtcgag cggggagacg acggtgtcgc
     1741 cgtgatcacc ctggacaacg gcaaggtcaa cgccctgagc atcgccctgc tcgaccaact
     1801 ccgggcggcg gccgaggacc tcaccgcgga cccaccgggc gcggtgatca tcaccggcgg
     1861 cgcccggatc ttcgccgccg gggcccagat caccgagttc ctggacgatc ccgatgcgct
     1921 gccctcacag gtcgccgacc agaaccggct gaccgcccag ggccacgcct tcctgatcgc
     1981 gctcaacgcg gtggccgcca tcccccgcgc caccatcgcc gaggtcggcg gcttcgccct
     2041 gggcggaggc tgtgagttgg cgttggcctg cgacttccgc atcgcttcgg accgggccaa
     2101 gctcggccag cccgagatcc tgttgggcat catccccggc ggtgggggca cgcagcgcct
     2161 gtcccgcctg gtcggcccgg ccaaggcgaa ggacctggtg ctcaccgggc gccaggtcgg
     2221 ggcggcggag gccctggcca tgggcctggt cgacgaggtc gtttccgccg aggagctcgc
     2281 cgcccacgtc cgggccaagg ccgcagccct ggccggtggc gccgtcctgg ctcaggcgct
     2341 ggccaagcgg gccatcgacg agggcctgga ccggggcctg gccgagggcc tggccctcga
     2401 gcaggagctc ttcatcgcct cgttcgccac cgacgacgcg ggaatcggca tcaacagctt
     2461 cctggagcac gggcccggcg aggcggactt cacgggccgc tgaacctcgg gcgtcctcag
     2521 acgttcacgt agaagctccg cgccgcgccg gctccgacca cgttgacggg ctgcacgcgg
     2581 accaggtgac ggccgggggg caggttccgc aggcggaagt ggcgctggc
//
LOCUS       DASTFV010000576         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2125628, whole genome shotgun sequence.
ACCESSION   DASTFV010000576 DASTFV010000000
VERSION     DASTFV010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2125628"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFP02_10555"
     CDS             <1..495
                     /locus_tag="VFP02_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET9543515.1"
                     /translation="TCFWVADRAALLSALSILPEYLRNEASASGAVIDYRDWQVPLGR
                     RFRALKLWFVIRHYGAEGLAHHVRRHVALAEELVGWVEADDRFALASPPRLGLVCLRH
                     VDGDGATQALLDAVNASGEQYLTHTRLDDRLVIRVSIGQTHTEERHVRALWDHVMSLA
                     DDLA"
     gene            500..889
                     /locus_tag="VFP02_10560"
     CDS             500..889
                     /locus_tag="VFP02_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543516.1"
                     /translation="MTVTVIFEGASHADIVDQVKTWLATVEEPDRLSASEVIEQGAGL
                     YKDALRIIATAAPRPVAENEIVKSLTDMGYRATDLTKDRLLDGLARVEELSGGTLVKD
                     VRDTGQKAVFQMNATLAKQILRQFTGG"
     gene            978..>2039
                     /locus_tag="VFP02_10565"
     CDS             978..>2039
                     /locus_tag="VFP02_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HET9543517.1"
                     /translation="MAEIEIGLGKSGRRAYGFDDIAIVPSRRTRDPEDVDISWEIDAY
                     RFELPLMGSAMDGVISPATAIEIGRLGGVGVLNLEGLWTRYEDPDVLFEEIADLDTEK
                     ATQRMQDIYQEPVKPELIGQRIREINEAGVTSCVSVTPQRTAAFAPYVLEAEADILVI
                     QGTVVSAEHVSKTAEPLNLKQFVRELTIPVIVGGCASYQAALHLMRTGAAGVLVGVGP
                     GHACTTRGVLGIGVPQATAIADARAARMRHLDETGVYVHVIADGGMGTGGDISKAIVC
                     GADAVMIGSPLAAAHEAPGRGYHWGMATFHPTLPRGARVKTTTRGTLQEILVGPAHEN
                     DGRMNLFGGLRTSMATCGYD"
BASE COUNT          321 a          784 c          644 g          290 t
ORIGIN      
        1 acgtgcttct gggtggcgga ccgggccgcc ctcctgtctg ccctgtcgat cctgcccgag
       61 tacctgcgca acgaggccag cgcatccggc gcagtcatcg actaccggga ctggcaggtc
      121 ccgctcgggc gccggttccg ggccctcaag ctctggttcg tgatccgcca ctacggcgcc
      181 gagggcctgg cccaccacgt gcgccgtcac gtcgccctcg ccgaggaact cgtcggatgg
      241 gtcgaggccg acgaccggtt cgctctcgcc tccccacccc gcctaggcct ggtgtgcctc
      301 cgccacgtcg acggcgacgg cgccacccag gccctgctcg acgccgtcaa cgcgtctggg
      361 gagcagtacc tgacccacac ccgtctcgac gaccgcctcg tcatccgggt ctcgatcggc
      421 cagacgcaca ccgaggagcg ccatgtccgg gccctctggg atcacgtcat gtccctggcc
      481 gacgacctag cgtgagccca tgacggtgac cgtcatcttc gaaggcgcct cgcacgccga
      541 catcgtcgac caggtcaaga cctggttggc caccgtggag gagccggacc gcctgtccgc
      601 gtccgaagtc atcgaacagg gggccggcct ctacaaggac gccctgcgca tcatcgccac
      661 ggccgccccc cgaccggtcg ccgagaacga gatcgtgaag tcgctcaccg acatgggcta
      721 ccgggccacc gacctgacca aggaccgcct gctcgacggc ttggcccggg tcgaggagct
      781 gtccggcggg acgctggtga aggacgtccg ggataccggc cagaaggctg tcttccagat
      841 gaacgccacc ctggccaagc agatcctccg ccagttcacc gggggttagc gagagggctc
      901 gaccatccac cggagccgtg cgcgcgcgtc actagactcc gaggtttcct accaacgcca
      961 agagctgggg agctgacgtg gcagagatcg agatcgggct cgggaagtcg ggacgtcggg
     1021 cctatggctt cgacgacatc gccatcgtgc ccagccgccg gacccgggac cccgaggacg
     1081 tcgacatcag ttgggagatc gacgcctacc gcttcgagct ccccctcatg ggctcggcca
     1141 tggacggggt catcagcccg gccacggcca tcgagatcgg gcgtctcggc ggcgtcggcg
     1201 tcctcaacct cgagggcctg tggacccgct acgaggaccc cgacgtgctg ttcgaggaga
     1261 tcgccgacct cgacaccgag aaggccaccc agcggatgca ggacatctac caggagccgg
     1321 tcaagcccga gctcatcggc cagcgcatcc gcgagatcaa cgaggccggc gtcacctcct
     1381 gcgtctcggt cacgccccag cgcaccgccg ccttcgcccc ctacgtgttg gaggccgaag
     1441 ccgacatcct ggtcatccag ggcaccgtcg tctccgccga gcacgtctcc aagaccgccg
     1501 agccgctgaa cctcaagcag ttcgtccgcg agctgaccat tccggtcatc gtcggcggct
     1561 gcgccagcta ccaggccgct ctgcacctca tgcgcaccgg ggccgccggt gtgctcgtcg
     1621 gcgtcggccc cggtcacgcc tgcaccaccc gcggcgtcct cggcatcggc gtcccccagg
     1681 ccacggccat cgccgacgcc cgcgccgccc gcatgcgcca cctcgacgaa accggcgtct
     1741 acgtccacgt catcgccgac gggggcatgg gcaccggtgg tgacatctcc aaggccatcg
     1801 tctgcggcgc cgatgcggtc atgatcggct cgcccctggc cgccgcccac gaggctcccg
     1861 gccggggcta ccactggggg atggccacct tccacccgac cctgccccgc ggcgcccggg
     1921 tcaagaccac cacgcggggc accctgcagg agatcctggt cggcccggcc cacgagaacg
     1981 acggccgcat gaacctcttc ggcggcctgc gcacctcgat ggccacctgc ggctacgac
//
LOCUS       DASTFV010000577         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2130704, whole genome shotgun sequence.
ACCESSION   DASTFV010000577 DASTFV010000000
VERSION     DASTFV010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2130704"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VFP02_10570"
     CDS             complement(<1..645)
                     /locus_tag="VFP02_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9543518.1"
                     /translation="MTAELDLLVLGSGVAGLSAAVRAAGPMGLRVGLLTKGALDQATT
                     RWAQGGVAAVLDEDPDSLDLHISDTLEAGADLCDAAAVRVLVDEGPRRVHELIAFGAV
                     FDRGAGGELALAREGGHSEPRIVHAGGAATGAEVERALVAAARATAAEVYEHTFAIDL
                     VVEGGRCRGVRAYGKDGHRLEIRARHTLLATGGAGQVFAVTTNPTEATGDGIAMA"
     gene            complement(690..1547)
                     /gene="panC"
                     /locus_tag="VFP02_10575"
     CDS             complement(690..1547)
                     /gene="panC"
                     /locus_tag="VFP02_10575"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET9543519.1"
                     /translation="MKASGSIAEVRWALDELRREGATVGLVPTMGYLHEGHLSLMAAA
                     RADTDFVMATIFVNPLQFAPGEDLASYPRDPDGDRAKAEVAAVDLLFSPEHDEMYPDE
                     VLTTVSVGGISQSMEGATRPTHFAGVATVVAKLFAIAGPCRAYFGEKDFQQLAVIRKM
                     VTDLSFPVEVIGCPIVREPDGLALSSRNAYLTPDQREAAPVLQRALKAGVATVRAGER
                     DPSAVCSLIGEIIAAEPEADLDYVALVDPATLEPPAGVLEPGTYRLLTAARFGIPRLL
                     DNIALEVPG"
     gene            complement(1572..>2044)
                     /locus_tag="VFP02_10580"
     CDS             complement(1572..>2044)
                     /locus_tag="VFP02_10580"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HET9543520.1"
                     /translation="GLDVLAGHPRPAALHPLVALPDADTGATRLADGAWFAVAGDPLV
                     ERVVAELGGQAFVVADEDRTRYHAAAVIASNHLVALLGQVDRVAPEGVPLEAFLALVR
                     GTVDNVAALGPAAALTGPVARGDWATVEAHLAALPDDERPAYQAMVDQARRLLD"
BASE COUNT          278 a          738 c          773 g          255 t
ORIGIN      
        1 ggccatggcg atgccatctc ccgtggcttc ggtcgggttg gtggtgacgg cgaagacctg
       61 gccggcgccc cccgtggcca gcagggtgtg cctggcccgg atctcgagcc ggtggccgtc
      121 cttgccgtag gcccgcaccc cccggcagcg tccaccctcg acgacgaggt cgatggcaaa
      181 ggtgtgctcg tacacctcgg ccgcggtggc gcgggcagcg gcgaccaggg cccgctcgac
      241 ctcggccccg gtggcggcgc ctcccgcgtg gacgatgcgg ggctcggaat ggccgccctc
      301 ccgggcgagg gccagctcgc cgccggcccc gcggtcgaag acagcgccga aggcgatcag
      361 ctcgtggacc cggcgggggc cctcgtcgac cagcacccgg acggcggccg catcgcagag
      421 gtcggccccg gcctcgaggg tgtcggagat gtgcaggtcg agggagtcgg ggtcctcgtc
      481 gagcaccgcg gccacgcccc cctgcgccca acgggtcgtg gcctggtcga gggcgccctt
      541 ggtcaacagg cccacgcgga ggcccatggg gcccgcggcc cgtaccgcgg ccgacaggcc
      601 tgccactcca ctcccgagga ccaggaggtc cagctcggcc gtcaccgtcc gaggacgcta
      661 cgcccgtccc tgtcgggaac cttgccgggt cagcccggga cctcgagggc gatgttgtcc
      721 aggaggcggg ggatgccgaa ccgggcggcg gtgagcagtc ggtaggtccc cggctcgagg
      781 acgccggcgg gaggctcgag cgtggcgggg tccaccagcg cgacgtagtc caggtcggct
      841 tccggctcgg cggcgatgat ctcgccgatc aggctgcaga ccgcggacgg gtcccgctcg
      901 ccggcccgga ctgtagccac gcccgccttc agggcccgct gcaggaccgg cgccgcctcc
      961 cgctggtccg gggtcaggta ggcgttgcga ctggacaagg ccaggccgtc gggctcgcgc
     1021 acgatcggac agccgatgac ctcgaccggg aacgacaggt cggtcaccat cttccggatc
     1081 accgccagct gctggaagtc cttctcgccg aagtaggccc ggcagggccc ggcgatggcg
     1141 aagagcttgg ccacgaccgt ggccaccccg gcgaagtggg tgggccgggt cgccccctcc
     1201 atcgactgcg agatcccgcc tacggaaacc gtggtgagga cctcatcggg gtacatctcg
     1261 tcgtgctccg ggctgaagag gaggtcgaca gcggccacct cggccttcgc ccggtcgcca
     1321 tcggggtccc ggggatagga cgccaggtcc tcccccggag cgaactgcag cgggttgacg
     1381 aagatggtgg ccatcacgaa gtcggtgtcg gcccgggccg cggccatcag cgacaggtgc
     1441 ccctcgtgga ggtagcccat cgtcggcacc aggccgaccg tggccccctc ccgccggagc
     1501 tcgtccagcg cccaccgcac ctccgcgatg ctcccgctgg ccttcacgac gccagtctgc
     1561 cggtccggcg ctcagtccag cagccggcgg gcctggtcga ccatggcctg gtacgcgggg
     1621 cgttcgtcgt cggggagggc ggcgaggtgc gcctcgacgg tggcccagtc accccgagcc
     1681 accgggcccg tgagggccgc cgccggaccg agagcggcaa cgttgtcaac ggtgccgcgg
     1741 acgagggcga gaaaggcctc gagcgggacg ccctccggag ccactcggtc gacctggccg
     1801 aggagggcga ccaggtggtt ggaggcgatc acggcggcgg cgtggtaacg cgtgcggtcc
     1861 tcgtcggcga ccacgaaggc ctggcccccg agctcggcca cgacgcgctc gaccagcgga
     1921 tcaccggcta cggcgaacca ggccccgtcg gccaggcggg tggccccggt gtcggcatcg
     1981 ggcagggcca cgagggggtg caacgccgcc ggcctcgggt ggccggcgag gacgtcgagg
     2041 ccca
//
LOCUS       DASTFV010000578         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2132221, whole genome shotgun sequence.
ACCESSION   DASTFV010000578 DASTFV010000000
VERSION     DASTFV010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2132221"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1572)
                     /gene="lipA"
                     /locus_tag="VFP02_10585"
     CDS             complement(22..1572)
                     /gene="lipA"
                     /locus_tag="VFP02_10585"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET9543521.1"
                     /translation="MAYADAHALQHGLFAHSADDHLLLLEHPHVFTLGPGGSLDHLLV
                     DPDAVGAELVHTDRGGDITYHGPGQLVGYPILTVPGKRGGGMADTVAYVRSVEQLLID
                     VLADLGLSDVGRVEGLAGVWVAPDSPRPRKVAAIGVKLSRGRSMHGFALNVDTDLAWF
                     DRIVPCGLVDKGVTSLRAEGLDVTMDEVVDAVAARAAERWGSAGWERADVKALPSDVP
                     VKVGGLAIAERKPEWLRAPVRLGEDYRALRKRMRRLDLVTVCEEAGCPNISECWSDGT
                     ATFMLNGERCTRACGFCLVDTRKPDAPPAADEPERVAAAVAEMGLRFAVLTAVARDDL
                     PDGGAAQFAATIEAIRRRNPATEVEVLIPDCKGAPAALDVIFAARPDVLNHNLETVAR
                     LQRAVRPSAGYARSLAVLARARAAGLTTKSGMILGLGETEDEVVAALGDLRGVGTDIV
                     TLGQYLRPTAAHLPVRRWWTPEEFDRLKAAGEALGLAHVEAGHLVRSSYHAREAAESG
                     HAALDDAG"
     gene            complement(1599..>2186)
                     /locus_tag="VFP02_10590"
     CDS             complement(1599..>2186)
                     /locus_tag="VFP02_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET9543522.1"
                     /translation="VRRAHRDSWKAQEGFSLTYLPFVARAVVDAIEDFPHLNASVGEG
                     ELLVHHAVHLAIAVDLDFEGLLAPVVRNTESKRLRAIAREIRDLADRAHQKKLSADEI
                     AGGTFTITNPGQYGTLLQFPVINQPQVAILSTDGVHRKPVVVTGPDGTEAITIHSVGV
                     LALAWDHRAFDGAYAAAFLARLKDTIETRDWEAEL"
BASE COUNT          303 a          768 c          826 g          289 t
ORIGIN      
        1 aggggccaga acgaacgggg gtcagccggc gtcgtcgagg gcggcgtggc cggactcggc
       61 ggcctcgcgg gcgtgatagc tcgaacgcac cagatggccg gcctccacgt gagcgaggcc
      121 gagggcctct cccgccgctt tcaggcggtc gaactcctcc ggggtccacc agcggcggac
      181 gggcaggtgg gcggcggtgg gacgcaggta ctggcccagg gtgacgatgt cggtgccgac
      241 gccccgcagg tcgcccaggg cggccacgac ctcgtcctcg gtctcgccca ggccgaggat
      301 catccccgac ttggtcgtga gcccggccgc ccgggcccgg gccagcaccg ccaggctgcg
      361 ggcgtagccc gccgaggggc gcacggcccg ctggaggcgg gccaccgtct cgaggttgtg
      421 gttcaggacg tcggggcggg cggcgaagat cacgtcgagc gccgccgggg cccccttgca
      481 gtccggaatc aggacctcga cctcggtggc cgggttgcgt cgccggatgg cctcgatggt
      541 cgccgcgaac tgggcggcac caccgtcggg caggtcgtcg cgggccacgg ccgtgagcac
      601 ggcgaagcgc aggcccatct cggccaccgc cgctgccacc cgctcgggct cgtcggccgc
      661 cggcggggcg tccggcttgc gggtgtcgac caggcagaag ccacaggccc gggtgcagcg
      721 ctcgccgttg agcatgaagg tggccgtgcc gtcggaccag cactcggaga tgttgggaca
      781 gcccgcctcc tcgcacacgg tgaccaggtc gaggcggcgc atgcgcttgc gcagggcccg
      841 gtagtcctcg cccagccgca ccggggcccg gagccactcc ggcttgcgtt cggcgatggc
      901 gaggccgccg accttcaccg gcacgtcgga gggcagagcc ttcacatcgg cccgctccca
      961 accggccgag ccccagcgct cagcggcgcg ggcggccacg gcgtcgacga cctcgtccat
     1021 cgtgacgtcg aggccttcgg cccgcaggga ggtcacgccc ttgtcgacga ggccgcacgg
     1081 cacgatgcgg tcgaaccagg ccaggtcggt gtccacgttg agggcgaagc cgtgcatcga
     1141 gcgcccccgg ctgagcttca ccccgatggc ggcgaccttt cgcggtcggg gggagtccgg
     1201 ggcgacccac acccccgcca ggccttcgac ccgacccacg tcggagagcc cgaggtcagc
     1261 gagcacgtcg atgagcagct gctcgaccga ccgcacgtag gcgaccgtgt cggccatccc
     1321 cccgccccgc tttccgggca cggtgaggat cgggtagccc acgagctgtc ccgggccgtg
     1381 gtaggtgatg tccccgcccc ggtcggtgtg gacgagctcg gcgccgaccg cgtcagggtc
     1441 gaccaggagg tggtcgaggg acccgcctgg ccccagggtg aagacatggg ggtgctccaa
     1501 caggagcagg tggtcgtccg cggaatgggc gaacaggccg tgctgcaggg cgtgggcgtc
     1561 ggcatacgcc acccgcccca accaccgcag gcgcagcact acagctcggc ttcccagtcc
     1621 cgggtctcga tggtgtcctt cagccgggcc aggaaggcgg cggcgtaggc gccgtcgaag
     1681 gcccggtggt cccacgccag tgccaggacg ccgacggagt ggatggtgat ggcctcggtg
     1741 ccgtcggggc cggtcacgac caccggcttc cggtgcacgc cgtcggtcga gaggatggcc
     1801 acctgcggct ggttgatcac cgggaactgc agcagcgtgc cgtactgacc ggggttggtg
     1861 atcgtgaagg tgccgccggc gatctcgtcg gccgacagct tcttctggtg agcccggtcg
     1921 gccaggtccc ggatctcccg ggctatggcc cgcagccgct tggactcggt gttgcgcacc
     1981 accggcgcca gcaggccctc gaagtcgagg tccacggcga tggccaggtg gacggcgtgg
     2041 tgcacgagca gctcgccctc gcccaccgag gcgttgaggt gggggaagtc ctcgatggcg
     2101 tcgaccaccg cccgggccac gaagggcagg taggtgaggc tgaagccctc ctgggccttc
     2161 caggagtccc gatgggcccg acggac
//
LOCUS       DASTFV010000579         3017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2147909, whole genome shotgun sequence.
ACCESSION   DASTFV010000579 DASTFV010000000
VERSION     DASTFV010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2147909"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2068
                     /locus_tag="VFP02_10595"
     CDS             <1..2068
                     /locus_tag="VFP02_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET9543523.1"
                     /translation="VLDAIAPTDRDERLEMVSVRTRRAVAGALALGLVASVVAVVGVP
                     PAAAEPVSRPEDPVVVTGRTLTPLFGVAPDRVAAFRREAGAWVPIPVQVDERAVVDFG
                     SQPPANGVPGSTGTVYGTQAIGVAVLQYTDPDTWVGPDPNAAVDRNDEVALMARDAGE
                     QALGAEPPEHVDAATGVELSITDPLEPGAQSWVYLFAHDGTLDPSAGQHLVDYDFSLA
                     SGDYKATYLRANGPNPEDSTVTTAAYVHHFSDRWVDDELHITVGGASGVDILDRHRNQ
                     FAPFECGRSEDTFSDDEGAFVVNRVGPVRAIRSYVGANSGPLSQRTHLFYEARHEILS
                     DLRVHPIPGMMELFDYSPAAAGMAYRNSAMGADVTIDGVPDTVPTAQNYWELVQGAQG
                     SLISVRRLTSDQQFPASARSTYYLDDATPPGDMCTGDTAAYGVSGLRLSASIANTDPR
                     STPSYRFRGRRVLTYRAPGATAVDARIVRRQVDTPLAVTARRLSDFPPHPFVDVDGLA
                     AISPALDWAAHHGLVTGFAGGEFRPDVAVRRGAAVNTLWHDVDEPTATAPHDFPDVRP
                     GAFYEDALSWAVEAGVVTGYPDGRFGPRDPVTRGQVVNLLWTTVGRPTGAPPHGFPDV
                     APGAFYGDALDWARDLGLVTGYPDGRYRPRDPVTRGQFVNMLFRLAGTPEGWTAFGPP
                     PSTVRF"
     gene            complement(2172..2801)
                     /locus_tag="VFP02_10600"
     CDS             complement(2172..2801)
                     /locus_tag="VFP02_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543524.1"
                     /translation="MALLLAVVVAAVACGGDDSTDEATADEASPASGPPPEWPAPPDP
                     LERTRAAGLEPELREQLQVHRHSHLDVFFNGDSVTVPAGVGIDISDPGVQTFDDPGGP
                     AYGGIEECENPCISPLHTHDVTGILHTESTSDELHTLGQFFVEWGVRLDDNCVGDYCA
                     PETDIAVYVNGDRVPGNPADIELADQTEIAIVIGSPPEDIPDTADFSPP"
BASE COUNT          410 a         1143 c         1036 g          428 t
ORIGIN      
        1 cgtgctcgac gccatcgcgc ccacggaccg ggacgagcgg ttagaaatgg tctccgtgcg
       61 gacgcgccgt gcggtggcgg gtgcattggc gctcggactg gtggcctccg tggtcgcggt
      121 ggtcggggtg cccccggccg cggccgagcc ggtgtcccgg cccgaggacc cggtcgtggt
      181 caccggccgg acgctcacgc cgctgttcgg ggtggccccc gaccgggtcg ccgccttccg
      241 ccgcgaggcc ggcgcgtggg taccgatccc ggtgcaggtc gacgagcggg ccgtcgtcga
      301 cttcggctcg caaccgccgg cgaacggcgt cccggggtcg accgggaccg tctacgggac
      361 ccaggccatc ggcgtggccg tcctgcagta caccgacccg gacacctggg tcggtcccga
      421 cccgaatgcg gccgtggacc gcaacgacga agtcgccctg atggcccgcg acgccggcga
      481 gcaggcgctc ggcgccgagc cgcccgagca cgtcgacgcc gccaccgggg tggagctgtc
      541 gatcaccgac ccgctcgagc ccggcgccca gagctgggtg tacctcttcg cccatgacgg
      601 cacgctcgac ccctctgccg ggcagcacct ggtcgactac gacttctcgc tcgcctccgg
      661 cgactacaag gccacctacc tccgggccaa cggtcccaac cccgaagact cgacggtcac
      721 gacggcggcc tacgtccacc acttctccga ccgctgggtc gacgacgagc tccacatcac
      781 ggtcgggggc gccagcgggg tcgacatcct cgatcggcac cgcaaccagt tcgccccctt
      841 cgaatgcggg cgcagcgagg acacgttctc cgacgacgag ggcgccttcg tcgtcaaccg
      901 ggtgggaccg gtccgggcca tccgctcgta cgtcggcgcc aacagcggcc cgctcagcca
      961 acgcacccac ctcttctacg aggcccgcca cgagatcctg agtgacctcc gggtccatcc
     1021 gatcccgggg atgatggagc tcttcgacta cagcccggcc gcggcgggca tggcctatcg
     1081 gaactcggca atgggagcag acgtcaccat cgacggggtc cccgacaccg tgcccacggc
     1141 ccagaactac tgggaactgg tgcagggggc gcagggttcg ctgatcagcg tgcgtcgcct
     1201 aacctccgac cagcagttcc ccgcctcggc ccgcagcacg tactacctcg acgacgccac
     1261 cccgccaggc gacatgtgca cgggcgacac cgcggcctac ggcgtcagcg ggctccgact
     1321 gagcgcctcg atcgccaaca ccgatccccg ctccaccccc tcctaccgct tccgcggccg
     1381 gcgggtcctg acctaccggg ctccgggggc gaccgcggtc gacgcccgca tcgtccgtcg
     1441 gcaggtcgac accccgctgg ccgtcaccgc ccggcgtctc tccgacttcc cgccccaccc
     1501 gttcgtcgac gtcgacggcc tggccgcgat ctcgcccgcc ctcgactggg cggcccatca
     1561 cgggctggtc accgggttcg cgggcgggga gttccgcccc gatgtggcgg tccgccgggg
     1621 tgcggcggtc aacacgctct ggcacgacgt cgacgagccc acggccacgg cgccgcacga
     1681 cttccccgac gtccgcccgg gggccttcta cgaggacgcg ctctcctggg cggtcgaggc
     1741 cggcgtggtc acgggctacc ccgacgggcg cttcggcccc cgggacccgg tgacccgggg
     1801 gcaggtggtc aacctcctct ggacgacggt cggccggccc accggcgccc cgccccacgg
     1861 gttccccgac gtggccccgg gggccttcta cggcgacgcc ctcgactggg cccgggacct
     1921 cgggctggtg accggttacc ccgacgggcg ctaccggcct cgcgaccccg tcacccgcgg
     1981 ccagttcgtg aacatgctgt tccggctggc ggggacgccc gagggctgga cggccttcgg
     2041 gccgccgccg tcgaccgtcc gcttctaggc gtactcggcg gcagcggcga gggcgtctcc
     2101 gaacgcctcg ccctcgcccg gtgcgcttcg ccgggcacac actcgacccc cgatggggga
     2161 cagcgcccag atcagggtgg tgagaagtcg gccgtgtcgg ggatgtcctc gggcggggag
     2221 ccgatgacga tcgcgatctc cgtctggtcc gccaactcga tgtccgcggg gttgccaggg
     2281 acgcggtcgc cgttcacata cacggcgatg tccgtctccg gagcgcagta gtcgccgacg
     2341 cagttgtcgt cgaggcggac tccccactcg acgaagaact gcccgagcgt gtggagctcg
     2401 tcgctggtcg actcggtgtg cagaatgccc gtcacgtcgt gggtgtggag gggagagatg
     2461 cacgggttct cgcactcctc gatcccacca taggcagggc cgccggggtc gtcgaaggtc
     2521 tgaacacccg ggtcgctgat gtcgatgccc actccggccg gcaccgtgac gctgtccccg
     2581 ttgaagaaca cgtcgaggtg cgagtgccgg tggacctgca gctgctcgcg aagctccggc
     2641 tccaagccag cggcgcgcgt gcgttccagg gggtccggcg gagctggcca ctcgggcggc
     2701 ggacccgacg cggggctcgc ttcgtctgcg gtggcttcgt ccgtcgagtc gtcgccgccg
     2761 catgccaccg cggcgacaac gacagccagg agcagcgcca cgagtctcct catcgaccgg
     2821 tgggtcccac cgcgcctccc ctcgggcacc gctgcgaacg gactgttggc ggagcgtcct
     2881 ccaccgcggc cccgagcgta atcgcgtagg tggacaccag ggtcgaaccg cggtggttgc
     2941 cgacccggtt acgccgaccg tgcgagtcga gatgtgaacg gccgccactg gttgcagcac
     3001 tcggtcaccc gacctag
//
LOCUS       DASTFV010000580         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2170981, whole genome shotgun sequence.
ACCESSION   DASTFV010000580 DASTFV010000000
VERSION     DASTFV010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2170981"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VFP02_10605"
     CDS             <1..512
                     /locus_tag="VFP02_10605"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9543525.1"
                     /translation="RWLLKAPSHLATLDALFAVYPDARIVHVHRDPLKTVPSTLSLMA
                     TLKWMRGRHTDPVATAPLLAWGFGALLDGVIDGRASGRLPDAQFADIRYADLLADPVA
                     AMAAIYEAFGWDYVPATRQAMLDHLAARPQDKHGTHRYALEDFGLDPAEERARFTPYQ
                     QHYQVPDEP"
     gene            complement(579..1268)
                     /locus_tag="VFP02_10610"
     CDS             complement(579..1268)
                     /locus_tag="VFP02_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET9543526.1"
                     /translation="MRIGRLVLVVACLLGAAVSCSSDGPTRVLLVGDSVMRQLGPALA
                     DQLPDDVRVRNEAVSGSGLLSPWFVDWPRNLDRVLDRYDADVVVFLFVGNYDLGGPRE
                     FETASGERIPDRTSPLFFRAWRAKAEQMTERAAEEADVTWILPPPMSGPDGQAVVDGL
                     RDVYRQLAEDTGAELIDAYDVLARPDGAFLREAPGPEGPPVPLRSPDGVHLARAGAAR
                     LATLVEDHLDL"
     gene            complement(1360..>2034)
                     /locus_tag="VFP02_10615"
     CDS             complement(1360..>2034)
                     /locus_tag="VFP02_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9543527.1"
                     /translation="PGRPSTPALVLGAFAGIAGSLGLVAYLRALATGPMGLVAPITSV
                     CGVTLPVVAGLVAGERLSIIATGGLVLGLVAIGIVAGGGGRTRAAAATGPLLAVIAGL
                     MIGTFLVLLDHTPGGSGLWPLVGARLASLTMLGTWAFATRAALPSRVNLRLIVASGVM
                     DMGANVLFLLATRAAPLTITSLLVSLSPVVIVLLARQVLAERLHRTQVAAVALSLVAV
                     AAVTIG"
BASE COUNT          315 a          752 c          740 g          227 t
ORIGIN      
        1 agcgatggct gctcaaggcg cccagccacc tggccacgct cgacgccctg ttcgccgtct
       61 accccgacgc ccgcatcgtc cacgtccacc gggacccgct gaagacggtg ccgtccaccc
      121 tgagcctgat ggcgacactg aagtggatgc gcggtcgcca caccgacccg gtggccacgg
      181 ccccgctgct ggcctggggc ttcggtgcgc tcctggacgg ggtgatcgac ggccgggcct
      241 ccggtcgcct gcccgatgcc cagttcgccg acatccgcta cgccgacctg ctggcggatc
      301 cggtcgccgc catggccgcc atctacgagg ccttcggctg ggactacgtc ccggcgaccc
      361 gccaggcgat gctcgaccac ctcgccgccc gcccccagga caagcacggc acccaccgct
      421 acgccctcga ggacttcggc ctcgaccccg ccgaggaacg cgcccgcttc accccctacc
      481 aacagcacta ccaggtcccc gacgagccct gatcacctcc gttctggaac ctgcggtttg
      541 tggtgggccg cgcgtttcag gttccaggac gagaggggtc agaggtcgag gtggtcctcg
      601 acgagggtgg cgaggcgggc ggcgccggct cgggcgaggt ggacgccgtc gggggagcgc
      661 agggggaccg gggggccctc cggccccggg gcctcgcgca ggaaggcgcc gtccggccgg
      721 gcgaggacgt cgtaggcgtc gatgagctcg gcgccggtgt cctcggccaa ctgccggtag
      781 acgtcccgga gaccatcgac cacggcctgg ccgtcaggac cggacatcgg cggcggcagg
      841 atccaggtga cgtcggcctc ctcggccgcc cgttcggtca tctgctccgc cttcgccctc
      901 caggcccgga agaagagcgg agaggtccga tcggggatcc gctcgcccga tgccgtctcg
      961 aactcgcggg gaccaccgag gtcgtagttg cccacgaaga ggaagacgac gacgtcggcg
     1021 tcgtaccggt cgaggacgcg atccaggttc ctcggccagt cgacgaacca cgggctcagc
     1081 aggcccgagc ccgagacggc ctcgttgcgg acgcgcacgt cgtcggggag ctggtcggcg
     1141 agcgccgggc cgagctggcg catgaccgag tcaccgacca gcagcacccg ggtcggtccg
     1201 tccgacgagc aggagaccgc cgccccgagc aggcaggcga ccaccaggac cagccgcccg
     1261 atgcgcatgc ccctacctgc ccgtccgagg gacccgcgat gcctcgttct gagacccacc
     1321 agccgtcacc ccgcggcaac tccgggtccc gtcgccccgt cagccgatgg tgacggcggc
     1381 gacggcgacc aagctgaggg cgacggcggc gacctgggtg cggtggaggc gttcggccag
     1441 gacctggcgg gccagcagca cgatgaccac gggcgacagg gacaccagca gcgaggtgat
     1501 ggtcagcggc gcggcccggg tggccaacag gaagagcacg ttggccccca tgtccatgac
     1561 cccggaggcc acgatcagcc gcaggttcac ccggctgggc agggcggccc gggtggcgaa
     1621 cgcccacgtc cccagcatcg tcagcgaagc caggcgggcg ccgaccagcg gccacaggcc
     1681 ggagccgccc ggggtgtggt cgagcaggac gaggaaggtc ccgatcatga ggccggcgat
     1741 cacggccagc agcggaccgg tcgccgcggc cgctcgggtc cgcccaccgc caccggccac
     1801 gatgccgatg gccaccaggc cgaggacgag gccgccggtg gcgatgatcg acaggcgctc
     1861 gcccgcgacc aggccggcca cgaccgggag ggtgacgccg cagaccgagg tgatcggggc
     1921 gacgaggccc atcgggccgg tggcgagggc ccgcaggtag gcgaccaggc cgaggctccc
     1981 ggcgatgccc gcgaaggcac ccagcacgag ggccggcgtc gacggacggc ccgg
//
LOCUS       DASTFV010000581         3117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2187285, whole genome shotgun sequence.
ACCESSION   DASTFV010000581 DASTFV010000000
VERSION     DASTFV010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2187285"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VFP02_10620"
     CDS             complement(<1..915)
                     /locus_tag="VFP02_10620"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HET9543528.1"
                     /translation="MTSAGISRRGFIGGTAGAGVVLVIGAKASAQAGGDGPYGALAAQ
                     PDANGLLLPEGFRSRVIARAGETVPGTPYEWPAFPDGAATFPIDAGGWYQVVNSEIPT
                     PGDGGASAIEYDADGEVVDAYRVLGGTTQNCAGGPTPWGTWLSCEEADGGQVWECDPT
                     TADSGAAMPAMGRFHHEAAAVDPENERVYLTEDESDGAFYRFTPDAYPDLTAGLLEVA
                     SVDPDGAVTWHEVPDPTGADTPTRQQVPEVTAFDGGEGCWYADGVVYFTTKGDDHVRG
                     LDTATDQMDVVYDGSGLLTGVDNITAEVG"
     gene            complement(992..1186)
                     /locus_tag="VFP02_10625"
     CDS             complement(992..1186)
                     /locus_tag="VFP02_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HET9543529.1"
                     /translation="MAEREQKRKTAPSRQEEEVEETPPTSESGEKIKAELDDLLDEID
                     EVLETNAEDFVKSYVQKGGQ"
     gene            complement(1204..2709)
                     /gene="dop"
                     /locus_tag="VFP02_10630"
     CDS             complement(1204..2709)
                     /gene="dop"
                     /locus_tag="VFP02_10630"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HET9543530.1"
                     /translation="MAINKILGVETEFGIIVRGTPESNPIAASSVLINAYVNELAGDP
                     QAGARVGWDFEDESPGNDARGMPPEGTMAPEVETHLVNVVLTNGARYYVDHAHPELST
                     PECGDARSIVRFDRSAEVILQRSMDAARRLLPPGQEIVVYKNNSDGKGNSYGTHENYL
                     MDRAVPFPRIVQHITPHFITRQIFCGAGKVGSEAPGLTTAEVPFQLSQRADFFEEEVG
                     LETTLKRPIVNTRDEPHADAQRYRRLHVIIGDANLSEVATYLKVGTTAIVLAMIEDDA
                     LPRDLRFARPVPTLRQISYDLTLRRAFELEDGSTITALDVQWELLDRARKYAESEGLA
                     VVGEEVGADVLTRWEAVLAGLESDPMGLADQLDWVAKYRLLDAYRERDGMAWSDTRLA
                     AMDLQYHDLRPEKSLFARLGMERLIDDDEIESGITEPPEDTRAYFRGTCLAKFADAIV
                     AANWDSMVFDVGTDPLRRVPMMEPSRGSKAHVGTLLEESKTAAELLDRLGS"
     gene            complement(2771..>3117)
                     /locus_tag="VFP02_10635"
     CDS             complement(2771..>3117)
                     /locus_tag="VFP02_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HET9543531.1"
                     /translation="EVTYQNGDKEVMFFKDFASGAMIENIVRRAKKLAIKRLIATGER
                     GIRTEDLLVSIHQEYKEHEDLPNTTNPDDWAKISGKKGERIVYVRTLISSNDDDSAGG
                     RSIERVGTGQYL"
BASE COUNT          420 a         1067 c         1094 g          536 t
ORIGIN      
        1 accgacctcg gcggtgatgt tgtccactcc ggtgagcagg ccggagccgt cgtagacgac
       61 gtccatctgg tccgtcgccg tgtcgagtcc tcgcacgtgg tcgtcgccct tggtggtgaa
      121 gtacacgacc ccgtcggcgt accagcagcc ctcgccaccg tcgaatgccg tcacctcggg
      181 cacctgctgg cgcgtcggcg tgtcagcacc cgtcgggtcc gggacctcgt gccaggtcac
      241 cgcgccgtcg gggtccacgg acgccacctc gagcaggccg gccgtcaggt cggggtaggc
      301 gtccggcgtg aagcgataga aggcaccgtc ggactcgtcc tcggtcaggt acacccgctc
      361 gttctcgggg tcgaccgccg cggcctcgtg gtggaagcgc cccatggccg gcatcgcggc
      421 gccgctgtcg gcggtggtgg ggtcgcactc ccagacctgt ccgccgtcgg cctcctcgca
      481 ggacagccag gtcccccagg gcgtcggtcc gccggcgcag ttctgggtcg tcccgccgag
      541 cacgcggtag gcgtcgacga cctcgccgtc ggcgtcgtac tcgatggcgc tggcgccgcc
      601 gtcgcccggt gtcgggatct cgctgttgac gacctggtac cagccgccgg cgtcgatcgg
      661 gaaggtggcg gccccgtccg ggaaggcggg ccactcgtac ggggtgccgg ggacggtctc
      721 gcccgcccgg gcgatgaccc gggagcggaa cccctcgggc agcagcagtc cgttggcgtc
      781 gggctgagcg gccagcgcac cgtagggacc gtcaccgccc gcctgggccg acgccttcgc
      841 cccgatcacg aggaccacgc ccgctcccgc ggtccccccg atgaaccccc gcctgctgat
      901 ccccgccgac gtcatggggg gaatccaagc acgtggcgcg gcctcagccg ccgcggtgct
      961 cagagagtgg tcatgacctc acgctatgag gtcactggcc gcctttttgc acgtagctct
     1021 tgacgaagtc ctcggcgttg gtctcgagga cctcgtcgat ctcgtccagg aggtcgtcga
     1081 gctcggcttt gatcttctcg ccggactccg acgtgggcgg ggtctcctcg acctcctcct
     1141 cctgtcggga aggagccgtc ttgcgcttct gctctcgctc agccatgcga tcaccctcgt
     1201 cgtctaggac ccgagtctgt cgagcagctc ggccgccgtc ttgctctcct ccaacaaggt
     1261 acccacgtgc gctttgcttc cgcgtgaggg ttccatcatc ggcacccgcc gcaacgggtc
     1321 ggtgccgacg tcgaagacca tcgagtccca gttggcggcc acgatggcgt cggcgaactt
     1381 ggccaggcag gtcccccgga agtaggcccg cgtgtcctcg ggcggctcgg tgatgcccga
     1441 ctcgatctcg tcgtcgtcga tcagccgctc catccccagc cgggcgaaca acgacttctc
     1501 cgggcggagg tcgtggtact gcaggtccat ggcggccagc cgggtgtcgc tccaggccat
     1561 gccgtcccgc tcgcggtagg cgtccaacag ccggtacttg gccacccagt ccagctggtc
     1621 ggccagcccc atcgggtcgg actcgaggcc ggcgagcacg gcctcccagc gggtgaggac
     1681 gtcggccccg acctcctcgc cgaccacggc caggccctcg gactcggcgt acttgcgggc
     1741 ccggtcgagc agctcccact gcacgtcgag ggcggtgatg gtcgagccgt cctccagctc
     1801 gaaggcccgg cggagcgtga ggtcatagga gatctggcgc agggtcggca cggggcgggc
     1861 gaagcgcagg tcccgtggga gggcgtcgtc ctcgatcatg gccaggacga tggcggtggt
     1921 cccgaccttc aggtacgtgg ccacctcgga caggttggcg tcgccgatga tcacgtgcag
     1981 gcggcggtag cgctgggcgt cggcgtgggg ctcgtcgcgg gtgttgacga tgggccgctt
     2041 cagcgtggtc tcgagaccga cctcctcctc gaagaagtca gcccgctggc tgagctggaa
     2101 ggggacctcg gccgtggtca ggcccggggc ctcgctaccc accttgcccg ccccacagaa
     2161 gatctggcgg gtgatgaagt ggggcgtgat gtgctggacg atgcggggga agggcaccgc
     2221 ccggtccatc aggtagttct cgtgggtccc gtaggagttg cccttgccgt cggagttgtt
     2281 cttgtagacg acgatctcct ggccgggggg caggaggcga cgggcggcgt ccatcgagcg
     2341 ctggaggatg acctcggccg accggtcgaa gcggacgatg gagcgggcgt ccccgcactc
     2401 gggcgtggac agctcggggt gggcgtgatc gacgtagtag cgggccccgt tggtcaggac
     2461 gacgttcacc aggtgggtct cgacctcggg ggccatcgtc ccctcggggg gcatcccccg
     2521 ggcgtcgttg cccggtgact cgtcctcgaa gtcccacccg acccgggcgc cggcctgcgg
     2581 atcgcccgcc agctcgttga cgtaggcgtt gatgagcacc gacgacgcgg cgatggggtt
     2641 cgactccggc gtgccccgga cgatgatccc gaactcggtc tcgaccccca ggatcttgtt
     2701 gatcgccacg aagggaccct acccaggcga cgaagccggt ggccgggcat cgacgccacg
     2761 gcgtcggcga ctagagatac tgcccggtac cgacccgttc gatggaacga ccgccggcgg
     2821 aatcgtcgtc gttggaggag atgagcgtgc gcacgtagac gatgcgctcc cccttcttgc
     2881 ccgagatctt ggcccagtcg tccgggttgg tcgtgttcgg gaggtcttcg tgctccttgt
     2941 actcctggtg gatcgagacc aggaggtcct cggtgcggat cccccgctcc ccggtggcga
     3001 tgaggcgctt gatggccagc ttcttggccc ggcgcacgat gttctcgatc atggcccccg
     3061 aggcgaagtc cttgaagaac atcacctcct tgtcaccgtt ctggtaggtg acttcga
//
LOCUS       DASTFV010000582         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2213040, whole genome shotgun sequence.
ACCESSION   DASTFV010000582 DASTFV010000000
VERSION     DASTFV010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2213040"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1995
                     /locus_tag="VFP02_10640"
     CDS             <1..1995
                     /locus_tag="VFP02_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HET9543532.1"
                     /translation="LSPGALGLVAYNKRAADEMGERLGDTRGLDIRTLNSLGLAILTG
                     RGPFAAPGSGQRQVDTLNEADQRRILDGLVDFPRRANTDPAAAWLEALGRVRLGLRPP
                     ADVEAEYAGDVDGLDEVFPRYQAELDRRGMVDFDQQIYGAVKLLLTDPEVRATAQRAS
                     RVLLVDEFQDLTPAHLLLLRLLAAPAFDVFGVGDDDQTIYGYNGADPTWLIDFDRYFP
                     GAVSHPLTVNYRCPPGVVAGARTLLTHNRRRVAKTIDAAPGRAGADTDLVVRVDDATV
                     AATVAVVQDRRAAGAATADIAVLTRVNATLAPVQVALDLAGVPCERVAGPAWLQRTGV
                     RSALAWLRLATTGGLRPVDLAEAVRRPPRGLSGKVIEWIAEQRTVEGIDRLAGRLSRD
                     RDREKVEAFADDLRAVTARAEGGTAAEVLAHVRDRLGLEGAMQSLDGSRGRVDRSSHT
                     DDLDALIQLAELHTDAATFGGWLANQLEARPATAGPVVHLSTVHRVKGLEWPHVVVHE
                     ASSRLFPHRLADDVEEERRVFHVAVTRGSATVTVVTPERAPSPFVAQLVTEAPPAPVE
                     RPGPAPIPTRAPPVAAAPERPAAPGDEGLRAALQAWRLDRSRADGVPAYVVFNNKTLD
                     DLVARRPADYAQLRACVGIGPAKVESYGDDLLALIAERSG"
BASE COUNT          263 a          828 c          745 g          266 t
ORIGIN      
        1 ctcagccccg gtgccctcgg cctcgtcgcc tacaacaagc gggcggccga cgagatgggt
       61 gaacgcctgg gcgacacccg gggtctcgac atccgcaccc tcaactccct cggcctggcc
      121 atcctcaccg ggcgaggccc cttcgccgcg ccgggcagcg gccagcgcca ggtggacacg
      181 ctgaacgagg ccgaccagcg ccgcatcctg gacggtctgg tcgacttccc ccggcgggcc
      241 aacaccgatc ccgccgcggc ctggctggag gccctgggcc gggtccgcct cggcctacgc
      301 ccgcccgccg acgtcgaggc cgagtacgcc ggcgacgtcg acgggctgga cgaggtcttc
      361 cctcggtacc aggccgagct ggaccggcgg ggcatggtcg acttcgacca gcagatctac
      421 ggggcggtga agctgctgct gaccgacccg gaggtccggg ccaccgccca gcgggccagc
      481 cgggtgctgc tggtcgacga gttccaggac ctgaccccgg cccacctcct gctcctgcgc
      541 ctgctggccg cccccgcctt cgacgtgttc ggcgtgggcg acgacgacca gaccatctac
      601 ggctacaacg gcgccgaccc cacctggctc atcgacttcg accggtactt cccgggcgcc
      661 gtgtcccacc cgttgaccgt caactaccgg tgcccgcccg gggtggtggc cggggcccgc
      721 accctgctga cccacaaccg ccgccgggtg gccaagacca tcgacgccgc gcccggtcgg
      781 gccggtgccg acaccgatct cgtcgtgcgg gtcgacgacg ccacggtggc ggccacggtg
      841 gccgtggtcc aggaccgtcg ggctgccggc gcggcgaccg ccgacatcgc cgtgctcacc
      901 cgggtgaacg ccaccctcgc tcccgtgcag gtcgccctcg acctggccgg ggtcccgtgt
      961 gagcgggtcg cgggtccggc ctggctgcag cgcacgggcg tgcgctcggc cctggcctgg
     1021 ctccggttgg ccacgacagg cggcctccgc ccggtcgacc tggccgaagc cgtgcgccgc
     1081 ccgccccggg gtctctcggg gaaggtgatc gagtggatcg ccgagcaacg caccgtcgag
     1141 ggcatcgacc gcttggccgg ccgactcagc cgggatcgcg accgggagaa ggtcgaggcc
     1201 ttcgccgacg acctccgggc ggtgaccgct cgggccgagg gcgggacggc ggcggaggtc
     1261 ctggcccacg tgcgcgaccg gctcggtctc gagggggcca tgcagtccct cgacggcagc
     1321 cggggccggg tcgaccggtc cagccacacc gatgacctcg acgccctgat ccagctggcc
     1381 gagctccaca ccgacgccgc caccttcggc ggctggctgg ccaaccagct cgaggcccgc
     1441 cccgccaccg cgggcccggt cgtgcacctg tcgaccgtgc accgggtcaa ggggctggag
     1501 tggcctcacg tcgtggtcca cgaggcctcg tcccgcctct tcccgcaccg gctggccgac
     1561 gacgtcgaag aggagcgccg ggtgttccac gtggcggtga cccggggctc ggccaccgtc
     1621 accgtcgtga cccccgaacg ggccccctcg cccttcgtcg cccaactcgt gaccgaggcg
     1681 ccgcccgcgc cggtcgaacg ccccggcccg gcgccgatcc cgactcgggc accgcccgtg
     1741 gccgcggcgc ccgaacgccc tgccgccccc ggcgacgagg gcctgcgggc cgccctgcag
     1801 gcttggcgtc tcgaccgctc gcgtgccgac ggcgtccccg cctacgtcgt cttcaacaac
     1861 aagaccctcg acgacctcgt cgcccgtcgg cccgcggatt acgcccagct gcgggcctgt
     1921 gtcggcatcg ggccggccaa ggtcgagagc tacggcgacg acctcctcgc cctcatcgcc
     1981 gagcggtcag gctgaggggg agcggtcggg gaggatcagg gtgaggtggg cgacggcgag
     2041 gcccatgagc aggacgccgt tggggatgtg ccaggccgcg gcctcgaggg tctcccggcc
     2101 ga
//
LOCUS       DASTFV010000583         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2216218, whole genome shotgun sequence.
ACCESSION   DASTFV010000583 DASTFV010000000
VERSION     DASTFV010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2216218"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VFP02_10645"
     CDS             complement(<1..718)
                     /locus_tag="VFP02_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET9543533.1"
                     /translation="MSPTPTGVPVLEDLEAALGGVDGKRILVRCDFNVPLDDGRIVDD
                     LRIRAALPTLHWLTERGAVVTAMSHLGRPKGPDPAFSLEPVRTRLGELAPGVTLLENL
                     RFDPGETADDPATVARLVKGQDGYVNDAFGSSHRAHASIVGPPRTLPSAAGRLLAREV
                     DVLLGIRTDPKRPFVAVLGGAKVSDKLGVIGAMLDVVDTLIVGGGMCFTFFAAQGKPI
                     GASLVEEDQIDDCRALLEQTD"
     gene            complement(715..1722)
                     /gene="gap"
                     /locus_tag="VFP02_10650"
     CDS             complement(715..1722)
                     /gene="gap"
                     /locus_tag="VFP02_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET9543534.1"
                     /translation="MSIRVGINGFGRIGRNLFRAAKKQGAEIDFVAVNDIGSVETMAH
                     LLKYDSVMGELPYEIKAVDGGISVDGDTLQVLAERDPAQLPWGDLGVDLVIESTGFFT
                     SREKASAHLEGGAPLVIVSAPCTDADNTIVVGVNDDTFDRSTQKVISNASCTTNCFVP
                     MVKVLDDAFGVEKGLMTTVHAYTGDQSLVDGPHSDLRRARSAAINIIPSSTGAARATG
                     LVLQAMQGKLDGTSLRVPVADGSVTDFVAVLKQDVTADEVNEAYHAAATSGPMADVLV
                     YTDEPIVSSDIVGTPASCTFDSRLTMAMGNLVKVLGWYDNEWGYSNRLVDLAKIVGTA
                     S"
     gene            complement(1797..>2645)
                     /gene="rapZ"
                     /locus_tag="VFP02_10655"
     CDS             complement(1797..>2645)
                     /gene="rapZ"
                     /locus_tag="VFP02_10655"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET9543535.1"
                     /translation="EFVIIAGLSGAGRSSAADDFEDLGWFVIDNLPPALIPKVAELAA
                     GPDATYDRVALAVGTTSDQAQVLPSVASLRAADTRVRVLFLDASTEVLVRRYEGSRRR
                     HPTAGDETSLGQAIAVEREMLEPVKAEADVVVDTSDLNIHQLRDRIRDLFGTDDPSAG
                     MQTTVVSFGYKHGLPLDVDLVLDCRFLPNPHWDEELRPLTGLDAPVRAFVLRQPETKA
                     FLSRLEDLLDLLLPAYEAEGKSYLTIAFGCTGGRHRSVAIAEEIATHLRRAGRRPTVT
                     HRDLDR"
BASE COUNT          375 a          880 c          980 g          410 t
ORIGIN      
        1 catcggtctg ctccagcaga gcccggcagt cgtcgatctg gtcctcctcg accagcgacg
       61 ccccgatcgg cttgccctgg gcggcgaaga aggtgaagca catcccgcca cccacgatca
      121 gggtgtcgac cacgtcgagc atggcgccga tcacgcccag cttgtcgctc accttggccc
      181 cgccgaggac ggcgacgaag ggccgcttcg ggtccgtgcg gatgccgagg aggacgtcca
      241 cctcgcgggc cagcaggcgg cctgcggcgc tgggcagcgt gcgaggcggg ccgacgatcg
      301 atgcgtgggc gcggtgcgag gacccgaagg cgtcgttgac gtagccgtcc tggcctttca
      361 ccaggcgggc cacggtcgcc gggtcgtcgg cggtctcgcc cgggtcgaag cgcaggttct
      421 ccagcagggt gaccccgggc gccaactcgc cgaggcgcgt cctcaccggc tcgagtgaga
      481 acgcggggtc cgggcccttg ggccggccca ggtggctcat cgccgtgacc accgcgccgc
      541 gctcggtcag ccagtgcagg gtcgggaggg cggcccggat ccggaggtcg tcgacgatgc
      601 ggccgtcgtc gagggggacg ttgaagtcgc atcggacgag gatccgcttg ccgtcgacgc
      661 cacccagggc ggcttcgagg tcctccagga cggggacgcc cgtgggcgtc gggctcaaga
      721 ggccgtgccg acgatcttgg cgaggtcgac caggcggttg gagtagcccc actcgttgtc
      781 gtaccagccg aggaccttca ccaggttgcc catggccatg gtcagcctcg agtcgaaggt
      841 gcacgaggcc ggggtgccaa cgatgtcgga ggacacgatg ggttcgtccg tgtagacgag
      901 cacgtcggcc atcggaccgg aggtcgcggc ggcgtggtac gcctcgttga cctcgtccgc
      961 ggtgacgtcc tgcttcagca cggcgacgaa gtcggtgacc gagccgtcag ccacggggac
     1021 ccgcagcgac gtgccgtcga gcttgccctg catggcctgc aggaccagac cggtggcccg
     1081 ggccgcgccg gtcgacgagg ggatgatgtt gatggccgcg ctccgggccc ggcgcaggtc
     1141 cgagtggggg ccgtccacga ggctctggtc gccggtgtag gcgtggaccg tggtcatcag
     1201 gcccttctcc acgccgaagg cgtcgtccag gaccttgacc atcggcacga agcagttggt
     1261 cgtgcacgag gcgttggaga tgaccttctg ggtcgaccgg tcgaaggtgt cgtcgttgac
     1321 cccgaccacg atggtgttgt cggcatcggt gcagggggcg gacacgatca cgagcggggc
     1381 tccaccctcg aggtgggccg aggccttctc cctcgaggtg aagaagccgg tggactcgat
     1441 gaccaggtcc acgccgaggt caccccacgg cagctgcgcc ggatcccgct cggccaggac
     1501 ctggagggtg tcgccgtcga ccgagatgcc gccgtcgacg gccttgatct cgtacgggag
     1561 ctcgcccatc accgagtcgt acttgaggag gtgggccatt gtctcgaccg agccgatgtc
     1621 gttgaccgcg acgaagtcga tctccgcccc ttgcttcttg gccgcccgga agaggttgcg
     1681 accgatgcgg ccgaagccgt tgatgccgac gcggatgctc atgctctgga gatctcctcg
     1741 atgagatggt tggtggggcg cgacggtgcg tcgcttctgg actcctgccg ccgaccctac
     1801 ctgtcgaggt cacggtgggt cacggtgggc cggcgcccgg ctcgccgcag gtgggtggcg
     1861 atctcctcgg cgatggccac cgaccggtgt cgcccgcccg tgcacccgaa ggcgatcgtc
     1921 aggtagctct tgccctcggc ctcgtaggcc ggcaggagca ggtcgagcag gtcctccaag
     1981 cggctcagga aggccttggt ctccggctgg cggagcacga aggcccgtac gggggcgtcc
     2041 aggccggtga gcggacgcag ttcctcgtcc cagtgcgggt tgggcaggaa gcggcagtcc
     2101 agcaccaggt ccacgtcgag gggcaggccg tgcttgtacc cgaaggagac caccgtggtc
     2161 tgcatgccgg ccgacgggtc gtcggtgccg aagaggtccc ggatccggtc ccggagctgg
     2221 tggatgttga ggtcgctggt gtcgaccacg acgtcggcct cggccttgac cggctccagc
     2281 atctcccgct cgacggcgat ggcctggccg aggctcgtct cgtcccccgc cgtcgggtgg
     2341 cggcgccggc tgccctcgta gcggcgcacc aggacctcgg tgctggcgtc caggaagagc
     2401 acccggaccc gcgtgtcggc tgcccgcagc gacgccaccg acggcagcac ctgggcctgg
     2461 tccgaggtcg tgcccaccgc cagcgccacc cggtcgtagg tggcgtcggg cccggcggcg
     2521 agttcggcca ccttcgggat gagggcgggc gggaggttgt cgatgacgaa ccaccccagg
     2581 tcttcgaagt cgtccgcggc cgatgaccgc ccggcccccg agaggccggc gatgatgacg
     2641 aactc
//
LOCUS       DASTFV010000584         4609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2220347, whole genome shotgun sequence.
ACCESSION   DASTFV010000584 DASTFV010000000
VERSION     DASTFV010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2220347"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VFP02_10660"
     CDS             complement(<1..877)
                     /locus_tag="VFP02_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET9543536.1"
                     /translation="MAMTVEHRDVVATGRHEGPGQGSHDDHADHGGHADHTEVFRRRF
                     WWSLLLTLPIVATSEMVMEWFRYDLDFPGIELVGPVLGTVVFLWGGWPFLSGGAQELR
                     ARQPGMMLLVAMAITVAYTASMATSLGWFDLEFWWELAALVTIMLLGHWQEMKALGQA
                     GDALAALAALLPDEAERVQPDGTVDVVSVGALAVGDLVLIRSGGRVPADGEVVEGSAD
                     VDESMITGESRPVAKGVGDRVIAGTVATDSALRVRVDAVGDETALAGIQRLVDEAQRS
                     TSRAQVLADRAAALLF"
     gene            complement(910..1383)
                     /locus_tag="VFP02_10665"
     CDS             complement(910..1383)
                     /locus_tag="VFP02_10665"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9543537.1"
                     /translation="MAPSRPRPGLTRILEVAPSGTHDLRRTVLRDGGDDEVVFAGDDE
                     RDTFHLGAVDEDGTTVGIVTFLERECPNRPDVHPARQLRGMAVAADRQGQGLGTALLR
                     AGIERCRAEGVAVVWAHARLTAVPWYEAHGLPAEGDVYVFGVMRLPHRLVVTDLG"
     gene            complement(1353..2756)
                     /locus_tag="VFP02_10670"
     CDS             complement(1353..2756)
                     /locus_tag="VFP02_10670"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET9543538.1"
                     /translation="MTDPTAIEIAEAVRSGERSAREVLDTYLARIDTGNEALNAFVHV
                     DPDLAGAAAARVDGQVAAGQDPGPFAGVPFGVKDLEDCAGMPTSHGSLPYMGRGGVAA
                     DSVHVARLRAAGAVPVGKTAAPEFGTLNFTKTKAWGVTRSPWNRDRTPGGSSGGSAAA
                     VAAGLVPMATASDGGGSTRIPAAFSGLVGMKPSHGRIPHPGPQDSQTSVYGLLTTTVA
                     ESARHLDVAAGPDDRDRLSLPSPGVGYESAIESTEVAGLRARWSPDMGFATVDPEVLD
                     IAGGAAEELASAASLVIDDDPVHLPDAVKVWLSAGAMDIWLDLEPGMWPDVADDLTFY
                     SRQSLEATENWVISRFANVKRRREALTEQAAALFADIEVLLTPTTAIPAFAAAGPPVA
                     VVDGQEVVGAMATPFTMLANLCWNPAISVPAGVTADGLPVGLQIVARRHRDEVPLRLA
                     RILEQARPWPRLAPAPA"
     gene            complement(2758..3459)
                     /locus_tag="VFP02_10675"
     CDS             complement(2758..3459)
                     /locus_tag="VFP02_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HET9543539.1"
                     /translation="MTAPEHDPPTEVDLVRWSEALSAIAKTGLGFSDNLYERERFEEV
                     LAVAADIRVASGRSFDTETQVEEWMKQVGDGVAGYVTPKVAVGAVVGDEDGRILLVQR
                     ADSGIWLYPTGWCDVGYSASEVVLKEVYEETGIEAEVVAPIAIFDGLRLGFTGIPLYS
                     IVFACRPIGGELKAHPLECRDVGWFAEDELPEPLAGADLWRTHAFRAINGEQVPVWFD
                     PPRRTPWREHPVEED"
     gene            complement(3456..4364)
                     /locus_tag="VFP02_10680"
     CDS             complement(3456..4364)
                     /locus_tag="VFP02_10680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9543540.1"
                     /translation="MRFSLWASLSNPWPDIRDGVQHAEATGWDGVYVADHFMADAGGP
                     FPPDSPNLEATAVVAGLAAATERVRLATLVVGITYRHPAVLAKWAATVDHLSAGRLLL
                     GLGAGWQENEHEQYGIPLGSPGERVDRCEEALQIIRGLLDEPLTTVDGDHYRVKDAIA
                     WPKPVQAHLPILIGAKGNRMLGRVATYADEWNMWSSPPELAERRAVLDQRCERIGRDP
                     GEIATSTQALVFVLEDNAKAEELQAAMGGRPCVAGTAERIAESVAAWRDVGVDELIVP
                     DFTLGTGSQRADALDTIIEHVAPAFR"
BASE COUNT          668 a         1659 c         1671 g          611 t
ORIGIN      
        1 agaacaggag agcggcagcc cggtcagcca ggacctgggc tcggctggtc gaacgttggg
       61 cctcgtccac cagtcgctgg atcccggcga gcgccgtctc gtcgcccacc gcgtcgaccc
      121 gcacccgcag agccgagtcg gtggccaccg tcccggcgat cacacggtca cccacgccct
      181 tggccacggg gcgcgactcg ccggtgatca tggactcgtc gacgtcggcg gagccttcga
      241 cgacctcgcc gtccgcaggg acccggccgc cggagcggat gagaacgagg tcgccgacgg
      301 cgagggcgcc gacggagacg acgtcgacgg tcccgtcggg ctggacccgc tcggcctcgt
      361 cgggcaacag cgcggccagc gccgccaggg cgtcgccggc ctggccgagg gccttcatct
      421 cctgccagtg gccgagcagc atgatcgtga ccagggccgc cagctcccac cagaactcga
      481 gatcgaacca cccgaggctc gtggccatcg acgccgtgta ggccacggtg atcgccatgg
      541 cgacgagcaa catcatcccc ggctgccggg cccgcagctc ctgggccccg cccgagagga
      601 acggccagcc accccagagg aacacgacgg tccccaggac gggcccgacc agctcgatcc
      661 ccgggaagtc gaggtcgtac cggaaccact ccatgaccat ctcgctggtg gccacgatcg
      721 gcagcgtcag caggaggctc caccagaagc ggcgacggaa cacctcggtg tgatccgcgt
      781 gacccccgtg gtcggcatgg tcgtcgtggc tgccctgccc ggggccctcg tgccggccgg
      841 tcgcaaccac atcgcggtgt tcgacggtca tagccatgcc ttatacccta ggggggtatc
      901 cctatatagt caacccaggt cggtcaccac gaggcggtgc gggaggcgca tcaccccgaa
      961 gacgtagacg tcaccctccg cggggagccc gtgggcctcg taccagggca ccgcggtgag
     1021 gcgggcgtgg gcccagacca cggccacccc ctcggcccgg cagcgctcga tcccggcccg
     1081 gagcagagcc gtgccgaggc cctgaccctg ccgatcggcg gccacggcca tcccccggag
     1141 ctgccgggcg gggtgcacat ccggacggtt cgggcactcc cgctccaaga aggtgacgat
     1201 gcccaccgtc gtgccgtctt cgtcgaccgc gccgaggtgg aacgtgtccc gctcgtcgtc
     1261 accggcgaag acgacctcgt cgtccccccc gtcccggagg accgtccggc gcaggtcgtg
     1321 cgtgcccgaa ggggcgacct cgaggatccg ggtcaggccg gggcgggggc gagacggggc
     1381 cacggacggg cctgctccag gatgcgggcc aggcgcaggg gcacctcgtc ccgatggcgg
     1441 cgggcgacga tctgcagccc gacgggaagg ccgtcggcgg tgacgccggc cggcaccgag
     1501 atggccgggt tccagcagag gttggccagc atggtgaacg gcgtggccat ggcccccacc
     1561 acctcctggc cgtccaccac cgcgacgggt ggtcccgcgg cggcgaaggc ggggatggcc
     1621 gtcgtcgggg tcaacaggac ctcgatgtcg gcgaacaggg ccgcggcctg ctcggtcagc
     1681 gcctcccggc gccgcttgac gttggcgaac cgggagatga cccagttctc ggtcgcctcc
     1741 aggctctgac gggagtagaa ggtcaggtcg tcggccacat ccggccacat gccgggctcc
     1801 aggtcgagcc agatgtccat ggccccggcc gacagccaga ccttcaccgc gtcgggcagg
     1861 tggacagggt cgtcgtcgat gaccaagctg gcggccgagg ccagctcctc ggccgcgccg
     1921 ccggcgatgt ccaggacctc ggggtcgacc gtggcgaagc ccatgtccgg cgaccagcgg
     1981 gcccgcaggc ccgcgacctc ggtgctctcg atggcggact catagccgac ccccggcgac
     2041 ggcaacgaga ggcggtcccg gtcgtcgggt ccggcggcga cgtcgaggtg gcgggccgac
     2101 tcggccacgg ttgtggtcaa caggccgtag acggaggtct ggctgtcctg cggccccgga
     2161 tgcgggatgc ggccgtggct gggcttcatg ccgaccaggc cggagaaggc cgccgggatg
     2221 cgcgtggacc ccccgccgtc gctggccgtg gccatcggca ccagcccggc cgcgaccgcg
     2281 gcggcggatc cgccactgga gccaccgggg gtgcggtccc ggttccacgg gctgcgggtc
     2341 acgccccagg ccttggtctt ggtgaagttg agcgtgccga actccggcgc cgccgtcttc
     2401 ccgaccggga cggccccggc cgcccgcaat cgggccacgt ggaccgaatc ggcggccacc
     2461 ccgccccggc ccatgtacgg caacgagccg tgcgaggtgg gcatgcccgc gcagtcctcc
     2521 aggtccttca cgccgaacgg gaccccggcg aacggcccgg ggtcctggcc ggcggcgacc
     2581 tgcccgtcga cccgggccgc cgcggcgccg gccaggtcgg ggtccacatg gacgaaggcg
     2641 ttgagggcct cgtttccggt gtcgatccgg gccaggtagg tgtccaggac ctcccgggcc
     2701 gaacgctccc ccgagcggac ggcctcggcg atctcgatgg cggtggggtc ggtcatctca
     2761 gtcctcctcg acggggtgtt cacgccacgg ggtgcggcgg ggcgggtcga accagaccgg
     2821 gacctgctcg ccgttgatcg cccggaaggc atgggtccgc cacagatcgg ccccggccag
     2881 cggctcgggc agctcgtcct cggcgaacca gcccacgtcc cggcattcga gagggtgggc
     2941 cttcaactca ccgccgatcg ggcggcaggc gaagacgatc gagtagaggg ggatgcccgt
     3001 gaagccgagc cgcaggccgt cgaagatggc gatgggggcg accacctcgg cctcgatccc
     3061 ggtctcctcg tagacctcct tgagcaccac ctcggaggcc gagtaaccga cgtcgcacca
     3121 gccggtcggg tagagccaga tgcccgagtc ggcgcgttgg accaggagga tgcggccgtc
     3181 ctcgtcgccc acgaccgcgc cgacggcgac cttgggcgtg acgtagccgg cgaccccgtc
     3241 gccgacctgc ttcatccact cctcgacctg ggtctcggtg tcgaaggacc gacccgacgc
     3301 cacccggatg tcggcggcga ccgccagcac ttcttcgaag cgctcccgct cgtacaggtt
     3361 gtcgctgaag cccagcccgg tcttggcgat ggccgacagg gcctcgctcc accggaccag
     3421 gtcgacctcg gtcggcggat cgtgctccgg agcagtcaac ggaacgccgg ggccacgtgc
     3481 tcgatgatcg tgtcgagggc gtcggcccgt tggctgccgg tccccaaggt gaagtcgggg
     3541 acgatcagct cgtcgacgcc gacatcccgc caagcggcca ctgactcggc gatgcgctcc
     3601 gcggtgccgg ccacacaggg ccggcctccc atcgccgcct gcagctcctc ggccttggcg
     3661 ttgtcctcga ggacgaagac gagggcctgg gtcgaggtgg cgatctcacc cgggtcccgg
     3721 ccgatgcgct cacacctctg gtccagcacg gcccggcgct cggccagctc cgggggtgag
     3781 gaccacatgt tccactcgtc cgcgtaggtg gcgacccgac ccagcatgcg gttgcctttg
     3841 gccccgatga ggatgggcag gtgggcctgg accggcttgg gccaggcgat ggcgtccttc
     3901 actcggtagt gatcgccgtc gacggtcgtc aacggctcgt cgaggaggcc ccggatgatc
     3961 tgcagcgcct cctcgcagcg gtcgacccgc tcaccgggcg agccgagcgg gatgccgtac
     4021 tgctcgtgct cgttctcctg ccagcccgcg ccgaggccga ggagcagacg gccggcgctg
     4081 aggtggtcga cggtggccgc ccacttggcc agcacggcgg ggtgtcggta ggtgatgccg
     4141 acgaccaggg tggccagacg gacccgctcg gtggccgcgg ccagcccggc cacgacggct
     4201 gtcgcctcca ggttcgggct gtcgggcggg aacgggccac cggcgtcggc catgaagtgg
     4261 tcggcgacgt agacgccatc ccatcccgtg gcctcggcgt gctggacgcc gtcgcggatg
     4321 tcgggccagg ggttgctcag ggacgcccac agcgagaagc gcatcccccg atgatcgcac
     4381 gctcgccaca gacccggccg cggcgcaggg gagcggagca gcaaagacga tcctcgacat
     4441 gggcggagcc gggtcagccc agggcggcga ggatggcctg ctcgacctgc gccaggcggt
     4501 cggcatcggt gaccttgtcg ccggacgcgg tccgcacgta gaacgagtcg acgacccggt
     4561 tgccgagggt ctggaccttg gccgtccgga tgtccaggcc gaggccggc
//
LOCUS       DASTFV010000585         2670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2223355, whole genome shotgun sequence.
ACCESSION   DASTFV010000585 DASTFV010000000
VERSION     DASTFV010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2223355"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..691)
                     /locus_tag="VFP02_10685"
     CDS             complement(128..691)
                     /locus_tag="VFP02_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9543541.1"
                     /translation="MTTATPDRAAAVRSALRRLVADHGLHGASMAAVAKEAGVATGTA
                     YVHYTSKDELLLAAYLELKVDLGRAAVDGLDRSAPAEARFTSMWRAVHGFLAADPTRA
                     RFLTQVESSPLAAAAHAAALDAGDDPVLDEAAASDLADRLRPLPPEVLWDLGFGPAVR
                     LVASGRPLDDAGLASLADACWQAITEA"
     gene            complement(688..1629)
                     /locus_tag="VFP02_10690"
     CDS             complement(688..1629)
                     /locus_tag="VFP02_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET9543542.1"
                     /translation="MSWSTKDIPDQTGRTAVVTGANGGLGLETAEALAAAGATVVLAA
                     RDPEKTERAEARIRRTVPEADLEVVALDLGSLDSVREAAAAIGAAHERIDVLVNNAGV
                     MGIPERATADGFEMQFGVNHLGHFALTSRLLPAVLRAEGRIVTVTSTAHHMGRRVDPD
                     NPHLKGRYGPWKAYGQSKLANYHFALGLHRRLLAASAGAGSLLAHPGLSNTDLQAHSV
                     TETGGGASQKFFETMAARTGMRPDQGALPQLRAATDPDAYSGQFYAPRFVNSGPPVRR
                     PVLRRLGLSRSVDVLWEVSERETGETLDVAAALSALR"
     gene            complement(1680..2405)
                     /locus_tag="VFP02_10695"
     CDS             complement(1680..2405)
                     /locus_tag="VFP02_10695"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET9543543.1"
                     /translation="MLGLMQVAVLLSPGVTAFEALGPYGVFRRVPGARVRLVADAPGR
                     IPTQGSSVALLADSVMEEYPDPDVVVVPGGIGIRRLVEDERALAWVAGAHESSEWTTA
                     VSTGSVLLAAAGVLEGDATTHWLATELLEEQGAHAVAERVVRSGKVLTAEGAAAAVEM
                     ALDVVARMVDPSTALRIREELDVDELGPMDPRRPVRPETLVALAGPDELDSEPAYVLG
                     PGTGAPKRRKAAKGRIEILFALT"
     gene            complement(2393..>2670)
                     /locus_tag="VFP02_10700"
     CDS             complement(2393..>2670)
                     /locus_tag="VFP02_10700"
                     /inference="COORDINATES: protein motif:HMM:NF023123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HET9543544.1"
                     /translation="SLEALADFVYGEEHRAIMVQRRQWFEKFESVYLALWWVRAGHVP
                     SIDEAKERLALLEANGPTPEAFTFRRPFPAPDEIVEVEADDDWRCSV"
BASE COUNT          370 a          958 c          999 g          343 t
ORIGIN      
        1 cctcgaccgc cctctcgccg accgccaagc ccaccgcgac gcccagctcg acgccgactg
       61 acgcgttctg atccaccccg cgggtcgcca gcgacgcaga agcgtcccca gaacggtgag
      121 gcgtgcctta cgcctcggtg atggcctgcc agcaggcatc ggcgagcgag gcgaggccgg
      181 cgtcgtccag cggtcgaccg gacgccacca ggcgaacggc cgggccgaag ccgaggtccc
      241 agagaacctc gggcggtagc gggcgcaagc ggtcagccag gtcagaggcg gcggcctcgt
      301 ccagcaccgg gtcgtccccg gcgtccaacg cggcggcgtg ggccgcggcc gccaggggtg
      361 aggactcgac ctgggtgagg aagcgagccc gcgtcgggtc ggcggcgagg aagccgtgga
      421 cggcccgcca catcgaggtg aatcgggcct cggccggtgc cgaccggtcc agcccgtcga
      481 ccgcggcgcg gccgaggtcg accttcagct cgaggtaggc ggcgaggagt agctcgtcct
      541 tcgacgtgta gtggacgtac gccgtcccgg tggccacccc ggcctccttg gccacggccg
      601 ccatcgatgc cccgtgcagg ccgtggtcgg cgaccaggcg tcgcagggcc gaccggaccg
      661 cggcagcccg gtccggggtg gcggtggtca acggagcgcc gacagggcgg cggcgacgtc
      721 gagggtctcg cccgtctccc gctcggagac ctcccacagc acgtcgaccg aacgggacag
      781 cccgaggcga cgcaggaccg gtcgccgcac cgggggcccg ctgttgacga agcggggcgc
      841 atagaactgg ccgctgtacg cgtccgggtc ggtggcggcc cgaagctgag gcagggcgcc
      901 ctgatcgggg cgcatgccgg tccgggccgc catcgtctcg aagaacttct ggctggcgcc
      961 gccgccggtc tcggtgacgc tgtgggcctg caggtcggtg ttggacaggc cggggtgggc
     1021 caggaggctg ccggcgccgg cgctggcggc gagcagacgg cggtgcagcc cgagggcgaa
     1081 gtggtagttg gccagcttgg actggccgta ggccttccac ggcccgtacc ggcccttcag
     1141 gtgggggttg tcgggatcga cccgccggcc catgtggtga gcggtgctgg tgacggtgac
     1201 gatccggcct tcggcccgca ggaccgcggg cagcaggcgg ctggtcaggg cgaagtggcc
     1261 caggtggttg accccgaact gcatctcgaa gccgtccgcc gtggcccgct cggggatccc
     1321 catgaccccg gcgttgttga cgaggacgtc gatgcgctcg tgggcggcac cgatggccgc
     1381 cgcggcctcg cggaccgaat cgagcgaccc gaggtccagg gccacgacct ccaggtccgc
     1441 ctcggggacg gtccggcgga tccgggcctc ggcccgctcg gtcttctccg ggtcgcgggc
     1501 ggcgaggacc accgtggccc cagcggcggc cagggcttcg gccgtctcca ggccgagccc
     1561 gccgttggcg cccgtcacga cggcggtgcg cccggtctga tcggggatgt ccttcgtgct
     1621 ccagctcatg ccccgctcct cactgaatga tcgttcattc agtataggtc gggacgaggt
     1681 caggtgaggg cgaagaggat ctcgatgcgg cccttggccg ctttgcgccg cttcggcgcg
     1741 ccggtgcccg ggcccaggac gtaggccggc tccgaatcga gctcgtcagg tccggccagg
     1801 gcgaccagcg tctcgggccg gaccgggcga cggggatcca taggccccag ctcgtcgacg
     1861 tcgagctcct cgcggatgcg cagcgcggtc gacgggtcga ccatgcgggc gacgacatcg
     1921 agggccatct cgaccgcggc cgccgccccc tccgcggtca gcaccttgcc cgaacggacg
     1981 acgcgctcgg ccacggcgtg ggctccctgc tcctcgagca gctcggtggc caaccagtgg
     2041 gtcgtggcgt cgccctcgag cacgccggcc gcggccagca ggaccgagcc ggtcgacacc
     2101 gccgtggtcc actcgctgga ctcgtgagcg ccggccaccc aggcgagagc ccgctcgtcc
     2161 tcgaccaggc ggcggatgcc gatgccaccg gggaccacga ccacgtccgg gtccgggtac
     2221 tcctccatga cgctgtcggc cagcagggcc acactcgacc cctgcgtggg gatacgaccc
     2281 ggcgcatcgg cgacgagtcg gacccgggcg ccgggcaccc ggcggaacac gccgtaggga
     2341 ccgagcgcct cgaaggcggt caccccgggc gagagcagca cggcgacctg catcagaccg
     2401 agcacctcca gtcgtcatcg gcctcgacct cgacgatctc gtcgggcgcc gggaagggtc
     2461 ggcggaaggt gaaggcctcc ggcgtcgggc cattggcctc gagcagggcg aggcgctcct
     2521 tggcctcgtc gatgctcggg acgtggccgg cgcgcaccca ccacagcgcc aggtagaccg
     2581 actcgaactt ttcgaaccac tgccggcgct gcaccatgat cgcccggtgc tcctcgccgt
     2641 agacgaagtc ggcgagggcc tccagcgacg
//
LOCUS       DASTFV010000586         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2224903, whole genome shotgun sequence.
ACCESSION   DASTFV010000586 DASTFV010000000
VERSION     DASTFV010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2224903"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1582)
                     /locus_tag="VFP02_10705"
     CDS             complement(164..1582)
                     /locus_tag="VFP02_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543545.1"
                     /translation="MGAVLVISTTLALVALIGTLIALVFIARGAGSGKEMDTYLTARD
                     SQTAVTLALAFLASGLGSWILFAPPEVGQFIGILGVIGYAVGGALPFVAYAWLGPKIR
                     DAAPDGVTLTDWVRQRFGRPAQAWVGLVSVFYMFMFITAELTAIGAVLDLLGGVDPWI
                     PIVLTAGVTAAYTAWGGLPASLRTDYVQGVMILVLVAIAMAAILIHVDDPLDRARDGG
                     LTTFSRAGWESIVVLSIAITAANLFHQGYWQRTWSAENTKVLARAGYGGALLSLLVLL
                     PIGATGMIAGGVAIAEGVEPSPVPFFFLLDGLPEIIIFLVAVMAVALVASSVDTLQSA
                     LAAMMARDLTDRRLTLNGAKVLTIILTVPAVLIALKGYDILRLFLIADLVAAATVVPV
                     FLGLRRPASSASVIAGSVAGLASVVILGWIQEGTLEGGIDLLTVATSPDLDVWSFVLA
                     PLVSGAVALAVGRVTVPSAPIS"
     gene            complement(1564..2364)
                     /gene="thiD"
                     /locus_tag="VFP02_10710"
     CDS             complement(1564..2364)
                     /gene="thiD"
                     /locus_tag="VFP02_10710"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET9543546.1"
                     /translation="MTPPVALTIAGSDSGGGAGIQADLKSFAALGVFGTSAITAVTAQ
                     NTRAVTRVDVLDPVTVEAQIDAVLADLPVAAVKTGMLATAEIVELVARRAAAGDLPHL
                     VVDPVMVASSGDRLLDEDAESAYRELLFPHAEVITPNLLEAAVIVGRSLTTVDDAASA
                     AQELAGEGTAWVVVKGGHLDGDATDLVVHRGELTRLTSPRVATANVHGTGCSFASAVA
                     AGLAQGLAPIDALGRAKAFIDRAVRGGATWRLGGGHGPIDHFGWGPSS"
     gene            complement(2361..2951)
                     /locus_tag="VFP02_10715"
     CDS             complement(2361..2951)
                     /locus_tag="VFP02_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET9543547.1"
                     /translation="MLTDRRQSAAAGRDLAETVAAVVAAGAPTVLFREKDLPADERRA
                     LGERVGEVCAAAGAQLTVASDPTLARMLGAGSVHLAGDDPWPAEDDLAVGRSCHSAGE
                     LADADTHGAAYATVSPVFATTSKPGYGPPLGLVGLRALVAGTRVPIVALGGVGPDDVG
                     VCLAAGATGVAVMGAVMTAADPGAVVRELLDHEPVR"
BASE COUNT          469 a         1060 c         1127 g          338 t
ORIGIN      
        1 ctcaccttca gcggcatcga acgagcccgc ctcgccctgg tcacggtgag cgggtccgac
       61 aaggccgagg ccctggcccg cacggtcgcg ggcgagacca cggcctccca gatccgggcc
      121 gagcaggtcg tgtggctggt cgaccgagag gccgccgctc agctcagctg atgggcgccg
      181 acgggaccgt cacgcggccg acggccagcg ccaccgcacc ggacaccagc ggggccagga
      241 cgaaggacca gacgtcgagg tcgggcgagg tggccaccgt cagcaggtcg atgccccctt
      301 ctagcgtgcc ctcctggatc cagccgagga tcaccacgga cgccagtccg gccaccgagc
      361 cggcgatgac cgaggcgctg gaggcgggcc ggcgcaggcc gaggaacacg gggaccacgg
      421 tcgccgccgc caccaggtcg gcgatgagga agaggcgcag gatgtcgtag cccttcagcg
      481 cgatgaggac cgcgggcacg gtgaggatga tggtgagcac cttcgcgccg ttgagggtca
      541 accggcgatc ggtcaggtcg cgggccatca tggcggcgag ggcgctctgg agggtgtcga
      601 cgctcgaggc cacgagggcg accgccatca cggccaccag gaagatgatg atctcgggca
      661 ggccgtcgag caggaagaag aacggcacgg gcgacggctc gacgccctcg gcgatggcca
      721 cgcccccggc gatcatgccg gtggccccga tgggcaggag cacgaggagg ctcaacaggg
      781 caccgccgta gccggcccgg gccaggacct tcgtgttctc ggctgaccag gtccgctgcc
      841 agtagccctg gtggaacagg ttggcggcgg tgatggcgat gctcagcacc acgatgctct
      901 cccaacccgc ccgggagaac gtggtcaggc cgccgtcgcg ggcccggtcc aaggggtcgt
      961 cgacgtggat gaggatggcg gccatcgcga tggcgacgag gaccaggatc atcacgccct
     1021 gcacgtagtc ggtgcgcaag gaggcgggca ggccacccca ggccgtgtag gccgccgtca
     1081 cccccgccgt caggacgatc gggatccacg gatcgacccc gccgagcagg tcgagcacgg
     1141 cgccgatggc cgtgagctcc gcggtgatga acatgaacat gtagaagacc gacaccaggc
     1201 ccacccaggc ctgcgcgggc cggccgaagc gctgccgcac ccagtcggtg agggtcacgc
     1261 cgtcgggggc ggcgtcacgg atcttcggcc cgagccacgc gtaggccacg aagggcaggg
     1321 ccccgccgac ggcatagccg atcacgccca ggatcccgat gaactgaccc acctcgggtg
     1381 gcgcgaagag gatccaggag cccagtcccg acgcgaggaa ggccagcgcg agcgtgaccg
     1441 cggtctggct gtcccgggcg gtgaggtagg tgtccatctc cttcccgctg ccggcgccac
     1501 gggcgatgaa gaccagggcg atgagggtgc cgatcagggc gacgagagca agggtggtgg
     1561 agatcacgag gacggccccc atccgaagtg gtcgatcggt ccgtgaccac caccgagccg
     1621 ccacgtcgcc ccgccgcgca ccgcccggtc gatgaaggcc ttggcccgcc ccagcgcgtc
     1681 gatgggcgcg agaccctggg ccagcccggc ggcgacggcc gacgcgaacg agcaacccgt
     1741 cccgtgcacg ttggcggtgg cgacgcgcgg gctggtcagg cgggtcagtt cgccgcggtg
     1801 gaccacgagg tcggtggcgt cgccgtcgag gtggccgccc ttgaccacga cccaggccgt
     1861 gccctccccg gccagttcct gggcggcgga cgcggcgtca tcgacggtgg tcagggagcg
     1921 ccccacgatc accgcggcct ccagcaggtt gggcgtgatg acctctgcgt gggggaagag
     1981 cagctcccgg tacgccgact cggcgtcctc gtcgaggagc cggtccccgc tgctggccac
     2041 catgaccggg tcgacgacca ggtggggcag gtccccagcg gccgcccgac gggccaccag
     2101 ctcgacgatc tcggccgtgg ccagcatccc ggtcttgacg gcggcgacgg ggaggtcggc
     2161 cagcacggcg tcgatctggg cctcgacggt cacggggtcg agcacgtcga ctcgggtcac
     2221 ggcccgggtg ttctgcgccg tgaccgcggt gatggcgctg gtcccgaaca ccccgagggc
     2281 ggcgaacgac ttgaggtccg cctggatgcc ggcgccgccg ccggagtccg agccggcgat
     2341 ggtgagggcg acgggcggag tcatcggacc ggctcgtggt cgagcagctc gcggaccacc
     2401 gcaccggggt cggccgcggt catgacggca cccatgacgg ccacaccggt ggcgccggcg
     2461 gccaggcaga ccccgacgtc gtcggggccg acgccgccga gggcgacgat cggcacgcgg
     2521 gtgccggcca ccagggcccg caacccgacc aggccgagcg ggggcccgta gccgggcttg
     2581 ctcgtggtgg cgaagacggg cgacacggtg gcgtaggccg ccccgtgcgt gtcggcgtcg
     2641 gccagctcgc ccgccgagtg acacgaacga ccgacggcga gatcgtcctc tgccggccag
     2701 gggtcatcgc cggccaggtg gacggacccg gcgccgagca tccgggccag cgtcggatcc
     2761 gaggccacgg tgagctgcgc ccccgccgcc gcgcagacct cgcccacgcg ctcccccagc
     2821 gcccgccgtt cgtcggccgg caggtccttc tcccggaaga gcaccgtagg ggcgcccgcg
     2881 gccacgacgg ccgcgaccgt ctcggccagg tcccgtccag ccgccgccga ctgccgtcga
     2941 tcggtgagca ccagcagcct cacaggggat cggggccgag caggtcaggc cgac
//
LOCUS       DASTFV010000587         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2230784, whole genome shotgun sequence.
ACCESSION   DASTFV010000587 DASTFV010000000
VERSION     DASTFV010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2230784"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1637)
                     /locus_tag="VFP02_10720"
     CDS             complement(<1..1637)
                     /locus_tag="VFP02_10720"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HET9543548.1"
                     /translation="MPSITIDPAIQADIEKFEVQLERYLSGDLAEDAFRVFRLNNGIY
                     GQRQGGHHQMVRVKLPYGSITPDQLDMFGHVADEYSRGWGHITTRQNIQLHFVPLERI
                     PAMMRDLASVGLTSREACGDTVRNVQGCHLAGACPYEILDISPWAEATFQHFLRHPLA
                     QRLPRKFKFNFSGCETDCGQAMFNDVGIIAAARRRDDGSIEPGFRVFVAGGLGANPHP
                     ALALEEFTTREDLMATLEACLRVFSNHGNRDNKLRARMKWLVDTLGWEELQAKILKER
                     HLLVASSSWPGGIPAIVEKLGDEPAGRADGVTPTTIGQGTPVSITRSQPYDRWEDANV
                     VRGSAKGTVSAYAWAKLGDITSAQFRSLAAFQREFGAEVRVTNRQNLVFRGLDESQLP
                     RLYERLATIGMAEPGAELSRDVVACPGADTCNLAVTQSRGLADAIGARLEEEGLAEIG
                     GIRTNISGCTNSCGQHHISDIGFSGAERRAHGQSAPGYTMLLGGYVGDAAINFGERAL
                     RLPAKNAPEATVRVMRRFASERQAGESFRGWMERSGGV"
     gene            complement(1787..>2238)
                     /locus_tag="VFP02_10725"
     CDS             complement(1787..>2238)
                     /locus_tag="VFP02_10725"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET9543549.1"
                     /translation="ATPDDVVALCEALNPDRVPGRLTLITRMGADHVAERLPPVLRAV
                     QEAGHPVVWACDPMHGNTYTSGERKTRHFDDICREIEGFFTAHRSVGTWPGGIHLELT
                     GEDVTECLGGGDHVSELDRRYETMCDPRLNAGQSLDLAFRVSEFLET"
BASE COUNT          336 a          709 c          840 g          353 t
ORIGIN      
        1 accccacccg agcgctccat ccagccgcgg aacgactcgc cggcctggcg ctcggaggcg
       61 aagcggcgca tgacgcggac cgtggcctcc ggggcgttct tggccggcag gcgcagcgcc
      121 cgctcgccga agttgatcgc cgcatcgccc acgtacccgc cgagcagcat cgtgtagccc
      181 ggggcggact ggccgtgggc ccggcgctcg gccccggaga acccgatgtc ggagatgtgg
      241 tgctggccgc acgagttggt gcagccggag atgttggtgc ggatgccgcc gatctcggcc
      301 aggccctcct cctcgaggcg ggcgccgatg gcgtcggcca gacctcggga ctgggtcacc
      361 gccaggttgc aggtgtcggc accggggcag gccacgacat cacggctgag ctcggcgccc
      421 ggctcggcca tgccgatggt ggccaggcgc tcgtagaggc gggggagctg ggattcgtcg
      481 aggccgcgga agacgaggtt ctgccggttc gtcacccgga cctcggcgcc gaactcccgc
      541 tggaaggcag ccagggaccg gaactgagcc gaggtgatgt cgccgagctt ggcccaggcg
      601 taggccgaca ccgttccctt ggccgagccg cggacgacgt tggcgtcctc ccagcggtca
      661 taaggctggg agcgggtgat gctgaccggg gtgccttggc cgatggtggt cggggtcaca
      721 ccgtcggccc gcccggcggg ttcgtcgccc agcttctcga cgatggccgg gatgccacca
      781 ggccacgagg aggaggccac gagcaggtgg cgttccttca ggatcttggc ctggagctcc
      841 tcccaaccga gggtgtcgac cagccacttc atgcgggccc gcagcttgtt gtcccggttc
      901 ccgtggttgc tgaacacacg gaggcaggcc tcgagcgtgg ccatgaggtc ctcgcgagtg
      961 gtgaactcct ccagggcgag ggcgggatgg gggttggccc cgagaccgcc ggccacgaac
     1021 acccggaacc cgggctcgat cgagccgtcg tcccgacgcc gggcggccgc gatgatgccg
     1081 acgtcgttga acatcgcctg gccgcagtcg gtctcgcagc ccgagaagtt gaacttgaac
     1141 ttgcggggca ggcgctgggc caggggatgg cgcaggaagt gctggaacgt ggcctccgcc
     1201 caggggctga tgtcgaggat ctcgtagggg caggcgccgg cgaggtggca gccctggacg
     1261 ttgcggacgg tgtcgccaca ggcctcccgg gaggtcaagc cgaccgaggc caggtcccgc
     1321 atcatggcgg ggatccgctc gagcgggacg aagtggagct ggatgttctg ccgggtggtg
     1381 atgtggcccc agccccggga gtactcgtcg gccacgtgcc cgaacatgtc gagctggtcg
     1441 ggggtgatcg agccgtaggg gagcttgacc cgcaccatct ggtggtgacc gccctggcgt
     1501 tggccgtaga tcccgttgtt cagccggaag acccggaagg cgtcctcggc gaggtcaccg
     1561 ctgaggtagc gctcgagctg gacctcgaac ttctcgatgt cggcctggat cgccggatcg
     1621 atggtgatgc tgggcatggg ggactttcgg gcagcggggt gggtgcgctc agagcgttca
     1681 aacccgagtg gtccactcag gattcccgat tatcctgaca aatccgatca ctttggtcaa
     1741 gatttactcg gtggaatcgg tcacgccttc ggggcccggg accggatcac gtctccagga
     1801 actccgagac ccggaaggcc aggtccaggg actggccggc gttgaggcga ggatcgcaca
     1861 tggtttcgta gcgacggtcg agctcggaca cgtggtcgcc gccgccgagg cactcggtga
     1921 cgtcttcgcc ggtcagctcg aggtggatgc caccgggcca ggtgccgacc gagcggtggg
     1981 cggtgaagaa gccctcgatc tcgcgacaga tgtcgtcgaa gtgccgggtc ttgcgctcgc
     2041 cgctggtgta ggtgttgcca tgcatcggat cacaggccca caccacggga tgcccggcct
     2101 cctgcacggc ccgcaggacc gggggcagcc gctcggccac atggtcggcc cccattcgcg
     2161 tgatcagggt gaggcgcccg gggacccggt cggggttgag ggcctcgcag agggcgacga
     2221 catcgtccgg ggtggcat
//
LOCUS       DASTFV010000588         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2242004, whole genome shotgun sequence.
ACCESSION   DASTFV010000588 DASTFV010000000
VERSION     DASTFV010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2242004"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /gene="groL"
                     /locus_tag="VFP02_10730"
     CDS             complement(<1..1324)
                     /gene="groL"
                     /locus_tag="VFP02_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779552.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET9543550.1"
                     /translation="MSKLLKFSEDGRRRLEAGVNKVADSVKVTLGPKGRNVVLDKKFG
                     APTVTNDGVSIAREIELEDNFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAMIREG
                     LRNVAAGANPMGLKKGIEKAVAAAVASIADQAKEIDDKSEIAQVASISSADPAIGEVI
                     ADAIDKVGKDGVVSVEESNTFGIELEFVEGMQFDKGYLSPYFVTDPDRQEAVLDEPYL
                     LFVNGKISSVQEMLPVLEKVMQAGKPLVIVAEDVEGEALATLVVNKIRGTFNSVGVKA
                     PGFGDRRKAMLQDMAILTGGQVISEEVGLKLDATTLDLLGTARKVVITKDDTTIVEGG
                     GAEGDVAGRIAQIRREIENTDSDWDSEKLQERLAKLAGGVALIQVGAATEVELKEKKH
                     RIEDAVSATKAAIEEGVVAGGGTALLRAKASVDAVVAKLKDDESTGARI"
     gene            complement(1330..1620)
                     /gene="groES"
                     /locus_tag="VFP02_10735"
     CDS             complement(1330..1620)
                     /gene="groES"
                     /locus_tag="VFP02_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET9543551.1"
                     /translation="MKLQPLEDRIVVKPGESEETTASGLVIPDTAQEKPQQGEVLAVG
                     PGKRSEQTGDVIPVDVSKGDTVVYSKYGGTEITIDGDDLLILSARDVLAIVK"
     gene            complement(1752..>2038)
                     /locus_tag="VFP02_10740"
     CDS             complement(1752..>2038)
                     /locus_tag="VFP02_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET9543552.1"
                     /translation="FQEAVVDVLVTKARKAADTVGAKDMALGGGVAANSRLREAFLAA
                     AAEDGRPGYVPSRAMCTDNAAMVASCGWYRWKADGGSPLDLGADPGLRLG"
BASE COUNT          277 a          713 c          677 g          371 t
ORIGIN      
        1 cgatgcgggc accggtggac tcgtcgtcct tcagcttggc gacgacggcg tcgaccgagg
       61 ccttggcccg gaggagggcg gtgccgccac cggcgaccac accttcctcg atggccgcct
      121 tggtcgccga gacggcgtcc tcgatgcggt gcttcttctc cttgagctcc acctcggtgg
      181 cggcgccgac ctggatcagc gccactccgc cggccagctt ggcgaggcgc tcctggagct
      241 tctcgctgtc ccagtcggag tcggtgttct cgatctcccg ccggatctgg gcgatccgac
      301 cggcgacgtc accctcggcg cccccaccct cgacgatggt ggtgtcgtcc ttggtgatga
      361 cgaccttccg ggcggtgccc agcaggtcga gcgtcgtggc gtcgagcttc agcccgacct
      421 cctcggagat gacctggcca ccggtgagga tggccatgtc ctgcagcatc gccttgcggc
      481 ggtcgccgaa cccgggggcc ttgacgccca ccgagttgaa cgtgccccgg atcttgttga
      541 ccacgagggt ggcgagggcc tcgccctcga cgtcctcggc cacgatgacc agcggcttgc
      601 ccgcctgcat caccttctcc aggacgggca gcatctcctg caccgagctg atcttgccgt
      661 tgacgaacag caggtagggc tcgtccagga cggcctcctg gcgatcgggg tcggtcacga
      721 agtacggcga caggtagccc ttgtcgaact gcatcccctc gacgaactcg agctcgatgc
      781 cgaacgtgtt ggactcctcg acagacacga cgccgtcctt gcccaccttg tcgatggcgt
      841 cggcgatgac ctcaccgatg gccgggtcgg cggacgagat ggaggcgacc tgggcgatct
      901 cggacttgtc gtcgatctcc ttggcctgat cggcgatcga cgccactgcg gcggccacgg
      961 ccttctcgat gcccttcttg aggcccatcg ggttggcgcc ggcggcgacg ttgcgcaggc
     1021 cctcgcggat catggcctgg gccaggaccg tcgccgtggt ggtgccgtcg ccggccacgt
     1081 cgttggtctt ggtcgccact tccttgacga gctgggcgcc catgttctcg aagttgtcct
     1141 cgagctcgat ctcccgggcg atggacacgc cgtcgttggt gacggtgggg gcgccgaact
     1201 tcttgtccag gacgacgttg cggcccttcg gccccagcgt caccttgacc gagtcggcca
     1261 ccttgttgac tccagcctcg aggcggcgtc ggccgtcctc agagaacttc agcagcttcg
     1321 acatgccggc tacttgacga tcgccagcac gtcgcgcgcg ctgaggatca ggaggtcgtc
     1381 gccgtcgatg gtgatctcgg tgccgccgta cttggagtag acgacggtgt cgcccttgct
     1441 cacgtcgacg gggatcacat caccggtctg ctcggaccgc ttgccgggac cgaccgccag
     1501 gacttcgccc tgctggggct tctcctgggc ggtgtcgggg atgaccaggc cagaggcggt
     1561 ggtctcttcg gactcgccgg gcttgacgac gatccgatcc tcgaggggct gaagcttcat
     1621 gtgctctcct cagtgtcttc ctggcgccgc gctggcgacc attagcactc tcactaggtg
     1681 agtgctaacg atacgcggcg ccccctcgga ggcgcaaccc gccgggccaa tcgccgccaa
     1741 gacttccgtg gtcagcccag ccggaggccg ggatcggcgc cgagatcgag cggggagccg
     1801 ccatcggcct tccaccggta ccagccgcag gacgccacca tcgccgcgtt gtcggtgcac
     1861 atggcccggc tgggcacgta gccaggacgg ccgtcttcgg ccgcggcggc caggaaggcc
     1921 tctcgcaggc gagagttggc cgccaccccc ccgccaaggg ccatgtcctt ggccccgacc
     1981 gtgtcggcgg ccttgcgggc cttggtcacg agcacgtcga ccaccgcctc ctggaacg
//
LOCUS       DASTFV010000589         4556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2247221, whole genome shotgun sequence.
ACCESSION   DASTFV010000589 DASTFV010000000
VERSION     DASTFV010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2247221"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /locus_tag="VFP02_10745"
     CDS             <1..1563
                     /locus_tag="VFP02_10745"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET9543553.1"
                     /translation="HRPRYDDWSLPKGKLEPGESWEDGARREVEEETGVTGVLGPHLA
                     TSTYTDTKGRPKEVRWFELCEPRGVFAANDEVDEVEWLSPDAARRRVTRRADVEVLQA
                     FDDHREGADPTVTTETAQSRRLPSLWRQVPNRPDDWFSADELDRARRYRKPVSRARIL
                     RLGISTVVLLVVVFADVAPRLIDALDITNWVVQLIVVVALLELISTVVSTPFDAWLEL
                     RHDREWELSTQTERGFAADLAKGLVLGFIINVVLLLPLLAVIRSTPTWWLWGWLVMVV
                     FTIGLGFLYPVVIAPIFNTFTRLEDEDMNDRVDRIADIAGVQISGTYVADESKRSRRD
                     NAYVAGLGATRRVVLFDTLLEHPPEVVEQVVAHEIGHWRLKHLRKQIPVIALLLLALF
                     AALKAFSGWDWIFEQAGVVTTGDIPLVGQPTALPLLVLAAQIGFGVLGLLTAWVSRAF
                     ERQADIEALDLLEQPQTMLDMQRGLHTKNLADLEPGLVKRLQASHPPAAERMALTSAW
                     AAAKGVTVSSSA"
     gene            complement(1547..2641)
                     /gene="arsB"
                     /locus_tag="VFP02_10750"
     CDS             complement(1547..2641)
                     /gene="arsB"
                     /locus_tag="VFP02_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HET9543554.1"
                     /translation="MTTTDPGPDLGSVAEELSTLDRFLPVWIGLAMAGGLLLGRLIPD
                     LNDGLERIKVDTVSLPIAIGLLAMMYPVLAKVRYGHLGDELADRRTLALSLVLNWLVG
                     PLLMFTLAWVFLSDLPELRTGLIIVGLARCIAMVLIWNDLACGSRELAAVLVAVNSLF
                     QIVMYSVLGWFYLSVLPDWLGLDTADLDVSIWAIAKSVLIFLGLPLLAGFLTRTIGEA
                     RRGREWYEETFLPRIGPVALYGLLFTIVVLFALQGEQITENPLDVARVAIPLLVYFAL
                     MWGGAFLTGRRLGLSYERNTSVAFTAAGNNFELAIAVAIAVFGVTSGQALAGTIGPLI
                     EVPALVGLVYVSLWAKGRYYATNATVTPTS"
     gene            complement(2673..3149)
                     /locus_tag="VFP02_10755"
     CDS             complement(2673..3149)
                     /locus_tag="VFP02_10755"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HET9543555.1"
                     /translation="MSRLQLALNVSDLDQAVDFYSKLFATEPAKRKPGYANFAIADPP
                     LKLVLFEGPDGGTINHLGVETETAAEVEAAEARLSDTGLETTGIDDTICCYATKVETW
                     VADPDGARWEWYVKTGDSDQLTNEIVGTGETAVCCTPAANEPVAVGAMTEDGPGVR"
     gene            complement(3231..3569)
                     /locus_tag="VFP02_10760"
     CDS             complement(3231..3569)
                     /locus_tag="VFP02_10760"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9543556.1"
                     /translation="MSERTFPLLDLAECCPTVLGAPLDDDEAAFLAHGFAALADPVRL
                     RLLSMIASADEVCSCEMQEPLGKSQPTVSHHTKTLAEAGLIVGEKRGRWVYWRIVPER
                     LDALRRALVS"
     gene            3691..4332
                     /locus_tag="VFP02_10765"
     CDS             3691..4332
                     /locus_tag="VFP02_10765"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HET9543557.1"
                     /translation="MTDQTTLTPDQTLAMKTAARHLKDEFSGVFGVETIENFLTTSYD
                     QFAGRANFPNFLPLLAERFARQRLHALAKVEGKAKSGVPVVLFLCVHNAGRSQIALGW
                     FNHLAADQAIAWSGGSEPVGEINRAAVDAMAEVGIDIAKEYSKPWTDEIVHAADVVVT
                     MGCGDACPIFPGKRYEDWDLEDPAGLGVEAVRPIRDEIEQRVRGLLDELSVTV"
BASE COUNT          726 a         1489 c         1611 g          730 t
ORIGIN      
        1 caccggcccc gctacgacga ctggtcgctg ccgaagggca agctcgagcc gggggagtcc
       61 tgggaggacg gggcccggcg cgaggtcgag gaggagaccg gtgtcacggg tgttctcggg
      121 ccccacctgg ccacctccac ctacaccgac accaaaggcc ggcccaagga ggttcgctgg
      181 ttcgagctct gcgagccccg cggcgtgttc gccgccaacg acgaggtgga cgaggtggaa
      241 tggctgtcac ccgacgcggc ccggcgtagg gtcacccgcc gcgccgatgt cgaggtcctt
      301 caggccttcg acgaccaccg cgagggagcc gacccgaccg tgacgaccga gaccgcccag
      361 tcccggcgcc tgccctcgct ctggcgacag gtgccgaacc ggcccgacga ctggttctcc
      421 gcggacgagc tggaccgggc ccgtcgctat cgcaagccgg tgtcccgggc ccggattctc
      481 cgcctgggga tctccaccgt ggtgctgctg gtcgtcgtgt tcgccgatgt cgccccccgt
      541 ctgatcgacg ctctcgacat caccaactgg gtggtccagc tgatcgtggt cgtggccctc
      601 ctcgagctca tcagcaccgt ggtgtcgacc ccgttcgacg cgtggctgga gctgcgccac
      661 gaccgggagt gggagctgtc gacccagacg gagcgggggt tcgcggccga cctggccaag
      721 ggcctcgtgc tcggcttcat catcaacgtg gtcctgctcc tgcccctcct ggcggtcatc
      781 cgcagcacgc cgacctggtg gctgtggggc tggctggtca tggtcgtgtt cacgatcggc
      841 ctgggcttcc tctatccggt cgtgatcgcc cccatcttca acaccttcac gcggctggag
      901 gacgaggaca tgaacgaccg ggtcgatcgc atcgccgaca tcgccggcgt ccagatctcc
      961 gggacctacg tggccgacga gagcaagcgg tcgcgccggg acaacgccta cgtcgccggc
     1021 ttgggcgcca cccgccgggt cgtgctcttc gacaccctcc tggagcaccc cccggaggtc
     1081 gtggagcagg tcgtcgccca cgagatcggc cactggcggc tcaagcacct gcggaagcag
     1141 atcccggtca tcgccctgct cctgctggcg ctgttcgccg ccctcaaggc gttctcgggc
     1201 tgggactgga tcttcgagca ggccggtgtg gtcaccaccg gggacatccc gctggtgggc
     1261 cagccgacgg ccctccccct gctcgtgctg gcggcccaga tcggtttcgg ggtgttgggc
     1321 ctgttgaccg cgtgggtgtc ccgggccttc gagcgccagg ccgacatcga ggccctggac
     1381 ctgttggagc agccccagac catgctggac atgcagcggg ggctccacac caagaacctg
     1441 gccgatctcg agccgggtct ggtcaagcgc ctccaggcca gccatccgcc cgccgccgag
     1501 cgtatggccc tcacctcggc ctgggccgcg gcgaagggcg tgaccgtcag ctcgtcggcg
     1561 tgactgtggc gttcgtggcg tagtacctgc ccttcgccca gagggacacg tacaccaggc
     1621 cgacgagggc cgggacctcg atgaggggcc cgatcgtgcc cgccagtgcc tgcccggagg
     1681 tgacgccgaa cacggcgatg gcgacggcga tggccagttc gaagttgttg cccgcggcgg
     1741 tgaacgccac cgaggtgttg cgctcgtagg aaaggccgag gcggcggccc gtcaggaacg
     1801 cgccccccca catgagggcg aagtagacga ggagggggat ggcgacccgg gcgacgtcga
     1861 gggggttctc agtgatctgc tccccctgga gcgcgaagag cacgacgatc gtgaagagca
     1921 ggccgtagag cgccaccgga ccgatgcggg ggaggaacgt ctcctcgtac cactccctgc
     1981 ctctgcgggc ctcgccgatg gtgcgggtga ggaagccggc gagcaacggg aggccgagga
     2041 agatgagcac cgacttggcg atggcccaga tcgagacgtc gaggtcggcg gtgtcgaggc
     2101 cgagccagtc gggaagcacc gacaggtaga accagccgag cacggagtac atgacgatct
     2161 ggaagagcga gttgacggcg acgaggacgg ccgccagttc ccggctgccg caggccaggt
     2221 cgttccagat gaggaccatg gcgatgcagc gggccagccc gacgatgatc aggcctgtgc
     2281 gcagctcggg caggtcggac aggaacaccc aggcgagggt gaacatcagc aacggcccga
     2341 ccagccagtt cagcaccagc gacagggcga gggtgcgacg gtcggccagc tcgtcgccga
     2401 gatggccgta gcggaccttg gcgaggacgg ggtacatcat cgccagcagc ccgatggcga
     2461 tgggcaacga gacggtgtcg accttgatgc gctcgaggcc gtcgttgagg tcggggatca
     2521 ggcggccgag cagcaacccg ccggccatgg ccaagccgat ccagaccgga aggaaccggt
     2581 cgagcgtgga cagctcctcg gcgaccgagc cgagatcggg gccgggatcg gtggtggtca
     2641 gcgcccggcc ccgctcggtt cggtgcgccc ggtcagcgga cgccgggccc gtcctcggtc
     2701 atggcgccga cggcgacggg ctcgttcgcc gctggggtgc agcagacggc ggtctcgcct
     2761 gtacccacga tctcgttcgt gagctggtcg ctgtcgccgg tcttcacgta ccactcccag
     2821 cgggccccgt cggggtcggc cacccaggtc tcgacctttg tggcgtagca gcagatcgtg
     2881 tcgtcgatgc cggtcgtctc gaggccggtg tcggagagac gggcttccgc ggcctcgacc
     2941 tcggccgcgg tctcggtctc gacgccgagg tggttgatgg tgccgccgtc gggaccttcg
     3001 aagaggacga gcttgagcgg cggatcggcg atggcgaagt tggcgtagcc gggcttgcgc
     3061 ttggcgggct cggtggcgaa cagcttggag tagaagtcga cggcctggtc gaggtcgctg
     3121 acgttgaggg cgagctggag acgggacacg ggttcctccg aagtggtcgt agatcgacag
     3181 atgtcgattg gtgtcactgt accaatcgat cgatgaatgt caatatgttc tcaggacacc
     3241 agcgcccggc ggagggcgtc gaggcgctcg gggacgatcc gccagtagac ccaacggccc
     3301 cgcttctcgc ccacgatcag gccggcttcg gccagagtct tggtgtggtg gctgacggtg
     3361 ggctgcgact tgccgagggg ttcctgcatt tcgcaggagc agacctcgtc ggcgctggcg
     3421 atcatgctga ggagccggag ccggacgggg tccgcgaggg cggcgaagcc atgcgccagg
     3481 aacgcggcct cgtcgtcgtc gagtggcgct cccagaacgg tcggacagca ctcggcgagg
     3541 tcgagcaggg ggaaggtccg ttcgctcatc tcgaatttcc gatccatgaa gaggtcaatt
     3601 gacagacgtc gatacattcg cgagagttga tcgatcgacc gcggcaaatg tatcagacca
     3661 cccccgatga ccaatccaga ggatccgtcc atgaccgacc agaccaccct cacccccgac
     3721 cagaccctgg ccatgaagac cgccgcccgc catctcaagg acgagttctc cggcgtcttc
     3781 ggcgtggaga ccatcgagaa cttcctgacc accagctatg accagttcgc tggtcgggcc
     3841 aacttcccga acttcctgcc gctgctggcc gagcgcttcg cccgtcagcg gttgcacgcg
     3901 ctggccaagg tcgagggcaa ggccaaatcg ggcgttcctg tcgttctctt cctctgcgtg
     3961 cacaacgccg gtcgctcgca gatcgcgttg gggtggttca accatctggc cgcggaccag
     4021 gccatcgcct ggagtggcgg ctcggagccg gtcggcgaga tcaatcgtgc ggcggtggac
     4081 gcgatggccg aggtcggtat cgacatcgcc aaggagtact cgaagccgtg gaccgacgag
     4141 atcgtccacg cggccgacgt tgtggtgacg atgggttgcg gtgacgcctg cccgatcttc
     4201 ccgggcaagc ggtatgagga ctgggacctc gaggatcccg ccggtctggg ggtcgaggcg
     4261 gttcgtccca tccgcgacga gatcgaacag agggttcggg gtctgctcga cgagctcagt
     4321 gtgacggtct gatcgttcgt cgtctgttca cctgggggcc acctgccggc cgggagccgg
     4381 aaagggggct tccttacctt cccctccgac agagaccgag ggcaccgcgc ccttcctcag
     4441 gaggacacct attccatgtt gcgcaacagc cgccttcggt ggctccttgg cctgctggcc
     4501 gcgttggcgc tcgtcgccgc ggcctgcggc gacgacagca gttccggcga tgacgg
//
LOCUS       DASTFV010000590         4056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2255971, whole genome shotgun sequence.
ACCESSION   DASTFV010000590 DASTFV010000000
VERSION     DASTFV010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2255971"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VFP02_10770"
     CDS             <1..702
                     /locus_tag="VFP02_10770"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET9543558.1"
                     /translation="RALGNALWDAVGAAGDDPDVDAVVLTGADPAFSAGVDLKEAAAG
                     GQGDADDAIGAVVESRQGPFPPHTKPLIGAVNGVAVTGGLEVALGCDFLIASDQARFA
                     DTHARVGVMPGWGLTVMLPQAIGVRRAREMSTTGNYVDAEQALAWGLVNHVVRHDELL
                     PFCRALAADIVSNDQPGVRQILGTYQQVVATTVAEGWEIEGRRAAAWPGNRWDPEEIA
                     KRRQAVVDRGRSQQA"
     gene            complement(710..1558)
                     /locus_tag="VFP02_10775"
     CDS             complement(710..1558)
                     /locus_tag="VFP02_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HET9543559.1"
                     /translation="MAYTCFDVDVTDGVAHLILKRADDLNTMTPEFWRELPEIVTGLS
                     ASGEARAVVLSSTGRHFTAGMDLSVFTGKSSLLGGADADAELGRQRSQLRLSAALLQD
                     SFTALERARMPVLAAIQGGCVGGGVDMVCACDCRYATSDAFFVVMETNIGMTADVGTL
                     QRLPKLIPEGVAREYAYTGRRMSAQRAVEVGLVNQLFDTHDDLVAGVLEIAAEIAAQS
                     PLAVWGTKEMITYGRDHTVADGLNYVATWQTGMFQGFDMMEAFAAQADKRDPEYQDLL
                     PEPGFD"
     gene            complement(1604..2611)
                     /locus_tag="VFP02_10780"
     CDS             complement(1604..2611)
                     /locus_tag="VFP02_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HET9543560.1"
                     /translation="MSDKTYDVVGLGSAIVDAITHADDAFVEEWGIDKGGMTLIDEQR
                     AHLLYDAMGPAAETSGGSAANTVAGVASFGGRAAFIGTVRDDQLGEIFRHDIRACGVD
                     YDVPAGTEGPSTARCLILVTPDAQRSMSTYLGISSLVDPANVDEALVASSKVVYCEGY
                     LWDMPQTIEAIKKAFDRAREAGGKVSFALSDGLCVERHREDFLRLVDERVDILFANEH
                     EICSLYPGDGFEDAIEDVRNHCEIAVLTRSEQGSVVVTADETLRIPAEPTEVLDTTGA
                     GDLYASGFLFGYTQGLDLTTCARLGSAAAAEVISHLGARPQIPLTEIVDQILVTESAE
                     P"
     gene            2700..>4056
                     /locus_tag="VFP02_10785"
     CDS             2700..>4056
                     /locus_tag="VFP02_10785"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET9543561.1"
                     /translation="MPDDNSADPYRLPRTVVPSRYDLTLRPDLDGAAFTGSVRIEVTV
                     EEPTATVVLNAADLGIDAAVVVAGDSRHTARAELDESTERLHLHLDEPLAAGDAAVEL
                     AFTGVLNDQLKGFYRSTFTDEGGHERVLATTQFEATDARQAFPCWDEPEFKARFAVTL
                     EVPDDLLAVSNAREVARQPLEDGWVRVHFAETMVMSTYLVAFVVGPLEVTDPVPAGGV
                     DLRVVHPVGKGHLARYALDVGAFCLDHLAGWYDIAYPGDKLDLVAIPDFAFGAMENLG
                     CVTFREVLLLVDPSTATQPELQNVVDVIAHELAHMWFGDLVTMRWWNGIWLNEAFATF
                     MEMVTTDAFRPDWERWTGFGVSRSEALDTDALHSTRPIEFEVTSPEDADGMFDVLTYE
                     KGAAVVRMLEQYLGEDRFREGIRRYLGAHQYANTETTDLWDAIEEATGEPTRRIMDSW
                     IF"
BASE COUNT          590 a         1441 c         1421 g          604 t
ORIGIN      
        1 cgcgcactcg ggaacgcgct gtgggacgcc gtcggcgcgg ccggcgacga tcccgacgtc
       61 gacgcggtcg tgctcaccgg ggccgatcct gcgttcagcg ccggcgtcga cctcaaggag
      121 gccgccgctg gtggccaagg cgacgccgac gacgccatcg gggccgtggt cgagtctcgg
      181 cagggcccgt tcccgcccca caccaagccc ctcatcggcg ccgtgaacgg cgtcgccgtg
      241 accggtgggc tggaagtggc cctgggctgt gacttcctca ttgcctccga ccaggcccgg
      301 ttcgccgaca cccacgcccg ggtcggggtc atgcccggct gggggctgac cgtcatgctg
      361 ccccaggcca tcggcgttcg ccgggcccgg gagatgagca ccaccggcaa ctacgtcgac
      421 gccgagcagg cgctggcgtg gggcctggtc aaccacgtcg tgcgtcacga cgagctcctc
      481 cccttctgcc gggccctggc cgccgacatc gtctccaacg accagccggg ggtgcgccag
      541 atcctgggca cctaccaaca ggtggtggcc accacggtgg ccgaggggtg ggagatcgag
      601 ggccggcgag cggcggcgtg gccgggcaac cgatgggacc cggaggagat cgccaaacgg
      661 cgtcaggccg tcgtcgaccg gggccggtcc cagcaggcct gagccccgcc tagtcgaacc
      721 ccggctcggg caggagatcc tggtactcgg ggtcccgttt gtcggcctgg gcggcgaagg
      781 cctccatcat gtcgaagccc tggaacatgc cggtctgcca cgtggcgacg tagttcaggc
      841 cgtcggccac cgtgtggtcc cggccgtagg tgatcatctc cttcgtgccc cagacggcga
      901 gcgggctctg ggccgcgatc tccgccgcga tctccagcac gccggcgacc aggtcgtcgt
      961 gggtgtcgaa gagctggttg accaacccga cctcgacggc ccgttgggcg ctcatccgcc
     1021 ggccggtgta ggcgtactcg cgggccacgc cctcggggat cagcttgggc aaccgctgca
     1081 gcgtgcccac gtcggccgtc atgccgatgt tggtctccat gaccacgaag aaggcgtcgc
     1141 tggtggcgta gcggcagtcg caggcgcaca ccatgtccac gccgccgccg acacagccgc
     1201 cctgaatggc ggccagcacc ggcatgcggg cccgctcgag ggcggtgaag ctgtcctgga
     1261 ggagggcggc gctcagccgg agctgggagc gctgccgccc gagctcggcg tcggcatcgg
     1321 cgccacccag cagcgacgac ttccccgtga agacggacag gtccatgccg gccgtgaagt
     1381 ggcgaccggt cgaggacagc acgacggcgc gggcctcccc cgaggcgctc aagccggtca
     1441 cgatctcggg cagctcgcgc cagaactccg gcgtcatcgt gttgaggtcg tccgcccgct
     1501 tcaggatgag gtgggcgacc ccgtcggtga cgtcgacatc gaagcaggtg taggccatcc
     1561 cgggaggcta ttgcccttcc ggcaagggct cagatccggc gaatcagggc tccgcgctct
     1621 cggtcaccag gatctggtcg acgatctcgg tcagggggat ctgggggcgg gccccgaggt
     1681 ggctgatgac ctcggccgcg gcggccgagc cgaggcgggc gcacgtggtc aggtcgaggc
     1741 cttgggtgta gccgaagagg aagcccgagg cgtagaggtc gccggccccg gtggtgtcga
     1801 ggacctcggt cggttcggcc gggatgcgca gcgtctcgtc ggcggtgacc acgaccgatc
     1861 cctgctcgga gcgggtgagc acggcgatct cgcagtggtt gcggacgtcc tcgatggcgt
     1921 cctcgaagcc gtcgccgggg tacagcgagc agatctcgtg ctcgttggcg aagaggatgt
     1981 cgacccgctc gtcgaccagg cggaggaagt cctcccggtg gcgttcgacg cagaggccgt
     2041 cggagagggc gaaggacacc ttgccgccgg cctcgcgggc ccgatcgaag gccttcttga
     2101 tcgcttcgat ggtctggggc atgtcccaga ggtagccctc gcagtagacg accttgctgg
     2161 aggccacgag ggcctcgtcc acgttggccg ggtcgaccag cgacgagatg cccaggtagg
     2221 tgctcatgga gcgctgggca tcgggcgtga cgaggatgag gcagcgggcc gtcgaaggtc
     2281 cctcggtccc tgcgggcacg tcgtagtcga cgccgcaggc ccggatgtcg tggcggaaga
     2341 tctcgcccag ctggtcgtcc cggacggtgc cgatgaaggc cgcccgccca ccgaaggagg
     2401 ccacgccggc caccgtgttg gccgcggatc caccggaggt ctccgccgcc gggcccatcg
     2461 cgtcgtacag caggtgggcc cgctgctcgt cgatgagcgt catgcccccc ttgtcgatcc
     2521 cccactcctc gacgaaggcg tcgtcggcgt gggtgatggc gtccacgatg gcgctgccga
     2581 ggcccaccac gtcataggtc ttgtcactca tgaggcgcgc gaggctaagg ggccaccggc
     2641 ggttccgccc aatggacgcc gcccggaggc cggtcgcccg gcgtcgctac cctcgccggg
     2701 tgcccgacga caactccgct gacccgtatc gcctcccgcg cacggtcgtg ccgtcccggt
     2761 acgacctgac gttgcgcccg gacctcgacg gcgcggcctt caccggcagc gtccgcatcg
     2821 aggtgaccgt cgaggagccc acggccacgg tcgtgctgaa cgcggctgac ctcgggatcg
     2881 acgcggcggt ggtcgtggcc ggcgacagcc gccacaccgc tcgtgccgag ctggacgagt
     2941 cgaccgagcg gctccacctc cacctcgacg agcccctggc ggcgggcgat gccgccgtcg
     3001 agttggcctt caccggtgtg ctcaacgacc agctcaaggg cttctaccgc agcaccttca
     3061 ccgacgaggg cggccacgaa cgggtcctgg ccacgaccca gttcgaggcc accgacgccc
     3121 gacaggcctt tccctgctgg gacgagccgg agttcaaggc ccgcttcgcc gtcaccctcg
     3181 aggtccccga cgacctcctg gctgtgtcca acgcccgcga ggtggcccgt caaccgctcg
     3241 aggacggctg ggtccgggtc cacttcgccg agaccatggt catgtcgacc tacctggtcg
     3301 ccttcgtggt cggtcccctc gaggtgaccg acccggtccc cgcgggcggg gtcgacctcc
     3361 gcgtcgtcca ccccgtcggc aagggccacc tggcccgcta cgcgctcgat gtcggagcct
     3421 tctgcctcga ccacttggcc ggctggtacg acatcgccta cccgggcgac aagctggacc
     3481 tggtcgccat ccccgacttc gccttcgggg ccatggagaa cctcggctgt gtgaccttcc
     3541 gcgaggtcct gctcctggtc gaccccagca ccgcgaccca gcccgagctg cagaacgtgg
     3601 tcgacgtcat cgcccacgag ctggcccaca tgtggttcgg cgacctggtg accatgcgct
     3661 ggtggaacgg gatctggctc aacgaggcct tcgccacgtt catggagatg gtcacgaccg
     3721 acgccttccg tcccgactgg gagcggtgga cgggcttcgg tgtctcccgc tccgaggccc
     3781 tcgacaccga tgccctgcac agcacccggc ccatcgagtt cgaggtcacc tcgcccgagg
     3841 acgccgacgg catgttcgac gtcctcacct acgagaaggg cgccgccgtg gtgcgcatgc
     3901 tggagcagta cctgggcgag gaccgcttcc gggagggcat ccgccgctat ctcggcgccc
     3961 accagtacgc caacaccgag accaccgacc tctgggacgc catcgaggaa gccaccggcg
     4021 agccgacccg gcggatcatg gacagctgga tcttcc
//
LOCUS       DASTFV010000591         2689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2256486, whole genome shotgun sequence.
ACCESSION   DASTFV010000591 DASTFV010000000
VERSION     DASTFV010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2256486"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /gene="rimP"
                     /locus_tag="VFP02_10790"
     CDS             <1..440
                     /gene="rimP"
                     /locus_tag="VFP02_10790"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET9543562.1"
                     /translation="LDLYDVEQHQATLRILVSKDGGIDIGALTELSRSISRALDDADP
                     VSGRYTLEVSSPGLERPLRRPDHFTGAVNDRITVKTVPGTEGERRVTGVLTAADEDGI
                     TVRPDEGPDAEERHLDYGEIEAARTLFDWGTTTKPTTKKKKAS"
     gene            437..1885
                     /gene="nusA"
                     /locus_tag="VFP02_10795"
     CDS             437..1885
                     /gene="nusA"
                     /locus_tag="VFP02_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET9543563.1"
                     /translation="MSGNNLEMMEALQALAVDKGISVDDLLAALADALESAYKRTPGA
                     YEYSWVTLDPDTFDIRVYAQDLDEDGEPEGDELDVTPDDFGRIAAQTAKQVMMQRIRE
                     VERDMKYEEYAGREGDIVTGMIQQSDQRYTLLDLGRVEALLPQAEQVPYERPEPNSRV
                     KAYIVEVRKTAKGPQIVVSRTHPGLIKRLFELEVPEIADGIVEIRACAREPGHRTKIA
                     VWSNDNNVDPVGACVGARGARVRMVVNELKGEKIDIVPFSDDAADFVMKALSPARVKE
                     VRIHEDTGTAEVIVPDYQLSLAIGKEGQNARLAARLTGWRVDIKSETQLAEEAAYQTD
                     DWAQGEWIIDPQSGEQMWQPADGTPAMTAEEWSQLGAEQAEAETAEGETTDTTADVTV
                     EPEADTAADATVAAEPEGDEPAADVTEDRPTAETVDEAPEPDAEDAVADGDEADAPAD
                     AVHAEEPAEGADEAVEAEDAAEADTSDVEATS"
     gene            2227..>2689
                     /locus_tag="VFP02_10800"
     CDS             2227..>2689
                     /locus_tag="VFP02_10800"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543564.1"
                     /translation="MSNQEVLDLCEKLGIGARSHSSSIVEAQGDRVRRRAQKDGLVKA
                     APPEEAKPVEEAKPKNPPVSTKKAPPKKKAEPKVEKTEPEPAPTPAPEPEAPAEVVEA
                     PEPAPAPEVEAPAAEAAEVVEAPAPEAPPADAPRRVVRSSGSSGPTRPVEPP"
BASE COUNT          481 a          975 c          943 g          290 t
ORIGIN      
        1 gcctcgacct gtatgacgtc gaacagcacc aggcgaccct ccggatcctc gtgagcaagg
       61 acgggggcat cgacatcggg gctctgaccg agctcagccg gtcgatctcc cgggccctgg
      121 acgacgccga tcccgtgtcg ggcaggtaca ccctcgaggt gtccagcccg ggcctcgagc
      181 ggcccctgcg ccgtcccgac cacttcaccg gagcggtcaa cgaccgcatc acggtgaaga
      241 cggtccccgg gaccgagggt gagcgccggg tgaccggcgt cctcaccgcc gccgacgaag
      301 acggcatcac cgttcgcccc gacgagggtc cggatgcgga ggagcgacat ctcgattacg
      361 gcgagatcga agccgcgcgg acgctgttcg actggggaac caccaccaag ccgacgacga
      421 agaagaagaa ggcctcatga gcggaaacaa cctggagatg atggaggcgc tgcaggcgct
      481 ggccgtcgac aagggcatct ccgtcgacga cctgctcgcc gccctcgccg acgccctcga
      541 gtcggcctac aagcgcacgc ccggggccta cgagtactcg tgggtcaccc tggaccccga
      601 cacctttgac atccgcgtct acgcgcagga cctggacgag gacggcgagc ccgagggtga
      661 cgagctcgac gtcaccccgg acgacttcgg ccgcatcgcc gcccagacgg cgaagcaggt
      721 gatgatgcag cgcatccgcg aggtcgagcg ggacatgaag tacgaggagt acgccggccg
      781 ggagggcgac atcgtcaccg gcatgatcca gcagagcgac cagcgctaca ccctgctcga
      841 cctcggccgc gtcgaggccc tcctccccca ggccgagcag gtgccctacg agcgccccga
      901 gcccaactcc cgggtcaagg cctacatcgt cgaggtccgt aagacggcca agggccccca
      961 gatcgtggtc agccgcaccc atcccggcct catcaagcgc ctgttcgagc tggaggtgcc
     1021 cgagatcgcc gacggcatcg tcgagatccg ggcctgtgcc cgcgagcccg gccaccgcac
     1081 gaagatcgcc gtctggtcca acgacaacaa cgtggacccg gtcggggcct gcgtgggcgc
     1141 ccggggtgcc cgcgtgcgca tggtcgtcaa cgagctcaag ggcgagaaga tcgacatcgt
     1201 ccccttctcc gacgatgccg ccgacttcgt catgaaggcg ctgtccccgg ctcgggtcaa
     1261 ggaggtgcgg atccacgagg acaccggcac cgccgaggtc atcgtgcccg actaccagct
     1321 gtcgctggcc atcggcaagg agggccagaa cgcccgcctc gccgcccgcc tgacgggctg
     1381 gcgcgtcgac atcaagagcg agacccagct ggctgaggaa gcggcgtacc agaccgacga
     1441 ctgggcccag ggcgagtgga tcatcgatcc ccagtccggc gagcagatgt ggcagcccgc
     1501 cgacggcacg ccggccatga ccgccgagga gtggagccag ctcggcgctg agcaggccga
     1561 ggccgagacc gccgagggcg agaccaccga cacgactgcg gatgtgaccg tggaaccgga
     1621 ggccgatacc gccgctgacg cgaccgtggc ggctgagccc gagggtgacg agcccgcggc
     1681 cgacgtcacc gaggaccggc ctacggccga gacggtcgac gaggcgccgg agcccgacgc
     1741 ggaggacgcg gttgcggacg gcgacgaggc ggacgccccc gccgacgccg tccacgccga
     1801 ggagccggcg gagggtgccg acgaggccgt cgaggccgag gacgccgccg aagcggacac
     1861 ctccgacgtc gaggcgacgt cgtaggccgc tccagccccc ggcggacctg catcggttgc
     1921 cgccgtacca cagggtcaga cgaactggtg cgggtgaccc gccagggcga cggatcgctg
     1981 gccgtgggac ctacactgcc gggccggggc gcgtggctgt gccgatcatc agccgcgtgc
     2041 ttcgacgccg cccagcggcg cggggccttc gcccgggccc tgcgagcgcc ggtcgcggac
     2101 gccgatgtcg aggcggtgcg tgaccaggtg ctcgaccggg cagacgcccg ccagaagcta
     2161 cgaaagggct gacacagcca ggtgccatcg aacgtacggg tctacgaact cgcccgggaa
     2221 ctcgggatga gcaatcagga ggtcctcgac ctctgtgaga agttgggtat cggcgcgcgc
     2281 agccactcgt cgagcatcgt cgaggcccag ggcgaccgtg tccggcgccg ggcgcagaag
     2341 gacggcctcg tgaaggccgc gccacccgaa gaggccaagc cggtcgaaga ggccaagccc
     2401 aagaacccgc cggtcagcac caagaaggcc ccacccaaga agaaggccga gcccaaggtc
     2461 gagaagaccg agccggagcc ggcgcccacc cccgcgcccg agcccgaggc gcccgccgaa
     2521 gtcgtcgagg cccctgagcc cgccccggcc cccgaggtcg aggcccccgc ggccgaagcc
     2581 gcggaggtgg tcgaggcccc cgcgcccgag gctccgcctg ccgacgcccc ccggcgcgtc
     2641 gtgcgctcga gcggcagcag cggcccgacc cgtcccgtgg aaccgcccc
//
LOCUS       DASTFV010000592         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2256943, whole genome shotgun sequence.
ACCESSION   DASTFV010000592 DASTFV010000000
VERSION     DASTFV010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2256943"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFP02_10805"
     CDS             <1..309
                     /locus_tag="VFP02_10805"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9543565.1"
                     /translation="AYSVPVWVGLLWVILLGTLAPYALEVAGLRYLTATTTGVVSMLE
                     PVIAAAVAWLWLEEVLNGVQLVGGALVLMGVGLVQVAGSRAVPDAEDGPFVVADVPLN
                     "
     gene            422..1132
                     /locus_tag="VFP02_10810"
     CDS             422..1132
                     /locus_tag="VFP02_10810"
                     /inference="COORDINATES: protein motif:HMM:NF027645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HET9543566.1"
                     /translation="MTIPPRDDDQVIVTTKGEVSEAAKAYAASKVGRLHQHAHGPVLL
                     TRVKLTYAEGEDVERNAIAEAAMDVDGRLVRGQVATHRIEEAVDLLVDRMIRQLDQAA
                     AKARTRERRPSGEPAPRPGRVIISPEEREVVAHKSFAIDRATLEEAAFDMEVLDYDFF
                     LFTEADDGRDKVVFRGPEGDVQLATGPPTETVEEALERLDAGGEPFVFFCDADTGRGA
                     VAYLRYDGHYGLIRPADG"
     gene            1230..1718
                     /locus_tag="VFP02_10815"
     CDS             1230..1718
                     /locus_tag="VFP02_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HET9543567.1"
                     /translation="MSFKVGDRVVYPHHGAAVIEKKEKRKTPGAESEEKEYLVLRMAH
                     GELTLSVPSDNAETVGMRRPIAREDVEDLYQVLAKRDVREPSNWSRRFKNHQEKLKSG
                     DVYQVAEVVRNLALREADRGLSAGEKSMLAKARSVLVSELSFALDVSEDDALDQVNAQ
                     LT"
     gene            complement(1711..>2277)
                     /locus_tag="VFP02_10820"
     CDS             complement(1711..>2277)
                     /locus_tag="VFP02_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HET9543568.1"
                     /translation="ACPLVGGDTSAGPALFIAVTVTGHADRPVLRSGAGPGETVFVTG
                     PLGAAAAALDRLEAGEPAPTEARLHHPLPRLDEGRAASSAGATAMVDLSDGLAVDVRR
                     IASASSVGIGLDPDTIPVAEGAALEQAVSGGDDYELFFTAPDPDRVLAAFERAGLPQP
                     MAIGLTIEGAEVTLGGQPLQGGWEHPLR"
BASE COUNT          343 a          785 c          828 g          321 t
ORIGIN      
        1 gcctactcgg tgccggtgtg ggtgggcctg ctctgggtga tcctgctggg cacgctcgcc
       61 ccctacgccc tggaggtggc cgggctccgg tacctcaccg cgacgacgac cggcgtcgtg
      121 agcatgctgg agcccgtcat cgcggccgcc gtggcctggc tctggttgga ggaggtcctg
      181 aacggggtcc aactggtcgg tggcgctctg gtgttgatgg gtgtcggtct cgtccaggtc
      241 gccggatccc gagccgtgcc cgatgcggag gacggcccgt tcgtcgtggc cgacgtgccg
      301 ctcaactagc tcgacgggcg gtcgggagca gctgaccgag cgggtgcggc ctgagcagat
      361 gaacggccgg gtttggtcac ctaacgtctg cagaggaccg gcatccacag gaaggctccg
      421 cgtgaccatt ccccctcggg acgacgacca ggtgatcgtg acgaccaagg gggaggtgag
      481 cgaagcggcc aaggcgtacg ccgcgagcaa ggtgggccgg ctgcatcagc acgcccacgg
      541 gccggtgctg ctcacccggg tcaagctcac ctacgccgag ggcgaggacg tggaacgcaa
      601 cgccatcgcc gaagcggcga tggacgtcga cgggcggctc gtgcggggcc aggtcgccac
      661 gcaccgcatc gaggaggccg tggacctgct cgtcgaccgc atgatccgcc aactcgacca
      721 ggccgcggcc aaagcccgca cccgcgagcg ccggccctcg ggggaacccg caccgcggcc
      781 gggtcgcgtc atcatctccc ccgaagaacg agaggtcgtg gcccacaagt ccttcgccat
      841 cgaccgggcc accctggagg aggcggcctt cgacatggag gtcctcgact atgacttctt
      901 cctcttcacc gaggccgacg acggccggga caaggtcgtc ttccggggcc ccgagggcga
      961 cgtccagctg gccaccggtc cgcccaccga gacggtggag gaggcgctgg agcgcctgga
     1021 cgccgggggg gagcccttcg tgttcttctg cgacgccgac accggccggg gtgcggtcgc
     1081 ctacctccgc tacgacggcc actacggcct gatccgccct gccgacggct gatccggtgc
     1141 ccgccggcgg tgggatcggg ccggaaatcg ggcatccttg aaggccgccg ccgcggtcgt
     1201 tgttgctttc ggaggagacc ccgccgccca tgtcgttcaa agtcggtgat cgtgtcgtgt
     1261 acccccacca tggggccgcg gtgatcgaga agaaggagaa gcgcaagacg ccgggcgccg
     1321 agtccgagga gaaggagtac ctcgtcctgc gcatggcgca cggcgagctg accctctcgg
     1381 tgccttccga caacgccgag accgtcggca tgcggcgccc catcgcccgt gaggacgtcg
     1441 aggacctgta ccaggtgctg gccaagcggg acgtgcgcga gccctccaac tggagccggc
     1501 gcttcaagaa ccaccaggag aagctgaagt cgggcgacgt ctaccaggtg gccgaggtcg
     1561 tccggaacct ggcgctgcgg gaggccgacc gcggcctctc cgccggcgag aagtcgatgc
     1621 tggccaaggc ccgcagtgtg ctcgtctccg aactgagctt tgcgctggac gtctccgagg
     1681 acgacgccct cgatcaggtc aacgcccagc tcacctgagc gggtgttccc agccgccttg
     1741 cagcggctga ccgccgaggg tgacctcggc gccttcgatg gtcaggccga tcgccatcgg
     1801 ctggggtagt cccgcccgct cgaacgcggc caacacgcgg tcgggatcgg gagcggtgaa
     1861 gaagagctcg tagtcgtcgc ccccgctgac cgcctgctcc agtgcggcgc cctcggcgac
     1921 ggggatggtg tcgggatcca gcccgatgcc gacgctcgac gcactggcga tgcggcggac
     1981 gtcgacggcg agcccgtcgg acaggtccac catcgccgtc gctcccgccg aagacgcggc
     2041 tcgtccttca tccaggcggg ggaggggatg atgcagcctt gcttccgtgg gggcgggttc
     2101 cccggcttcg agacggtcca gggccgccgc cgcggcgccc agcgggccgg tcacgaacac
     2161 ggtctcgccc gggccggcgc ccgagcggag gaccggccgg tccgcgtggc cggtcacggt
     2221 cacggcgatg aacagcgcgg ggcccgccga cgtgtcacca ccgacgagcg ggcaggc
//
LOCUS       DASTFV010000593         3491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2258748, whole genome shotgun sequence.
ACCESSION   DASTFV010000593 DASTFV010000000
VERSION     DASTFV010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2258748"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VFP02_10825"
     CDS             <1..374
                     /locus_tag="VFP02_10825"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9543569.1"
                     /translation="DLKPGGKYECFATDEMKEYSPGMPDVIVDGEVVEVDPPNRLVQT
                     YRMHFDPSQVEEGFTTVTWEIRAEGDNLSRLTVTHDVTDAPVMAATIVADGALNEGGG
                     GWAWIISDLKTLLETGEANEL"
     gene            complement(443..1183)
                     /locus_tag="VFP02_10830"
     CDS             complement(443..1183)
                     /locus_tag="VFP02_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9543570.1"
                     /translation="MSRVAVVVGGASGLGEAISEKLSDDGFFVVVADMDVDGAALVAG
                     RISGVAVEVDVRDDAAVAAMVDRAAAFGPLRTLVLSAAVETRAPLLETTDEAWREVID
                     TNLKGPFLCLRHAVPRMVEAGGGSVIALGSTLGMIVAPSYPAYSASKFALTNLCKQVA
                     IEHAPDGIRVNVVAPSATEVGLFARVAEATDDPEGIKAFVAGNVPMGRLGTADDVTAL
                     VAFLASDQSSYLSGAVIPLDGGMAARRL"
     gene            complement(1180..1965)
                     /locus_tag="VFP02_10835"
     CDS             complement(1180..1965)
                     /locus_tag="VFP02_10835"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9543571.1"
                     /translation="MAERTTDDRLLEGKVALITGSASGQGRAAALRFARHGAAIAIVD
                     IADDGSAETARQVEALGGEATVIHADVSVQADVEAMVTATVERWGRLDVLYNNAAVQM
                     SGRLVECSEADWDRTIATNLDAVFWACRAALPAMLAGEGGSIINTASVLGLIGSEGYV
                     AYGAAKAGLVALTRQTAVEYGPSVRANVIAPGSIDTPRFQKVAAEMDDPEGFVAGLAK
                     TIPLHRLGTAEDVASIALFLASDLSAYVSGAVIPADGGLAVYR"
     gene            complement(1958..3277)
                     /locus_tag="VFP02_10840"
     CDS             complement(1958..3277)
                     /locus_tag="VFP02_10840"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET9543572.1"
                     /translation="MGDSNVTVGLDIGTTSVKGLAVDDDGHVLAQVRVPHPILTPAAD
                     RFEHDANRAWRDGVRQAWRQVSSGLHPVGVNVVAMVPSLTAVDARGHALTPGLLYGDA
                     RGRGAARPTGAPGDVGELLGFLRWCHGEAPDAAGYWPAQAVANHALGGRGAMDSISAM
                     TAYPLYDGQGWDEAMLAESGVTADRLPDVVNGDSPVGEIDGAAIGPGGIDAFGEQLVA
                     GAEEPGDVLVICGTTLITWAVTDEWVEVPGLWTIPHSSPGRTLIGGPSNAGGLFLNWA
                     THLLARGDDPRPDRIPVWSPYVRGERTPLHDPDRRSGLDGLDLTQGPAALRRAAFEAA
                     GFVVRHHLDLAGVPVHRLVLTGGGVRVSEWVQALADCTGLPADVVAIPEGGALGSAFF
                     ARLAAGLETDATAARRWARVDHRVEPDPAWVEPCARRYQRFRELADG"
BASE COUNT          514 a         1262 c         1259 g          456 t
ORIGIN      
        1 acgacctgaa gccgggcggg aagtacgagt gcttcgccac tgacgagatg aaggagtact
       61 cgccgggcat gcccgacgtc atcgtcgacg gtgaggtggt cgaggtcgac ccgccgaacc
      121 gcctcgtgca gacctatcgc atgcacttcg acccgtccca ggtcgaggag ggcttcacca
      181 ccgtcacctg ggagatccgg gccgagggcg acaacctcag ccggctgacc gtgacccatg
      241 acgtgaccga cgccccggtc atggccgcca cgatcgtcgc cgacggtgcg ctcaacgagg
      301 gcggcggcgg ttgggcctgg atcatcagcg acctcaagac cctgctggag accggcgagg
      361 ccaacgagct ctgacccccc tgcgcttctg gcacccgatt cgactccccc aggagccgga
      421 tcgggtgcca gttcgcgtct cgctacaggc ggcgggccgc catgcctccg tcgaggggga
      481 tgacggcacc gctgaggtag ctcgactggt cggaggccag gaaggcgacg agggcggtga
      541 cgtcgtccgc ggtgccgagg cggcccatgg ggacgttgcc ggccacgaag gccttgatgc
      601 cctcggggtc atcggtggcc tcggccaccc gggcgaacag gccgacctcg gtggccgagg
      661 gggcaaccac gttgacccgg atgccgtcgg gggcgtgttc gatcgccacc tgcttgcaca
      721 ggttggtgag ggcgaacttg ctggccgaat aggcggggta gctcggggcc acgatcatcc
      781 cgagggtcga gccgagggcg atgaccgagc cgccgccggc ctcgaccatc cggggcacgg
      841 cgtggcgcag gcagaggaac gggcccttga ggttggtgtc gatgacctcc cgccacgcct
      901 cgtcggtggt ttccaggagc ggggcacggg tctcgacggc agcagagagg acgagggtcc
      961 tcaacgggcc gaaggcggcg gcccggtcga ccatcgccgc caccgcagcg tcgtcgcgga
     1021 cgtcgacctc gacggccacc ccgctgatgc ggcccgccac cagcgccgcg ccgtcgacgt
     1081 ccatgtcggc caccaccacg aagaacccgt cgtcggacag cttctcgctg atggcttcgc
     1141 ccaggccgga cgccccgccg accacgaccg cgacgcggct caccggtaca ccgccagccc
     1201 gccgtcggcc gggatgaccg cgccggagac gtaggccgac aggtcactgg ccaggaagag
     1261 ggcaatggag gccacgtcct cggccgtgcc cagccggtgc aacgggatgg tcttggccag
     1321 cccggcgacg aagccttcgg ggtcgtccat ctccgccgcc accttctgga agcggggggt
     1381 gtcgatggat ccgggggcaa tgacgttggc ccgcacggac ggtccgtact cgacggctgt
     1441 ctgtcgggtg agagcgacca gaccggcctt ggccgcgccg taggccacgt agccctccga
     1501 gccgatgaga ccgaggaccg aagcggtgtt gatgatcgag cccccctccc ccgccagcat
     1561 cgccggcaag gcggctcggc aggcccagaa gacggcgtcg aggttcgtgg cgatcgttcg
     1621 gtcccagtcc gcctcggagc actcgaccag ccggcccgac atctgcaccg cggcgttgtt
     1681 gtagagcacg tcgagccggc cccaccgctc gaccgtcgcc gtcaccatcg cctcgacgtc
     1741 ggcctggacg gacacgtcgg cgtggatcac cgtcgcctca ccgccgaggg cctcgacctg
     1801 tcgggcggtc tccgccgacc cgtcatcggc gatgtccacg atggcgatcg ccgcgccgtg
     1861 ccgggcgaag cgcagggccg ccgcccgtcc ctgccccgac gccgaccccg tgatcagcgc
     1921 caccttgcct tcgagcagcc ggtcatccgt ggtgcgctca gccatcggcc aactcgcgga
     1981 agcgttggta gcggcgggcg cagggctcga cccaggcggg gtcaggctcg acccggtggt
     2041 cgacgcgggc ccaacgccgg gcggcggtgg cgtcggtctc cagcccggcc gccaggcggg
     2101 cgaagaaggc cgatcccagg gcgccccctt cagggatggc gacgacgtca gcaggcaggc
     2161 ccgtgcagtc ggccagagcc tggacccact cggagacgcg caccccgccg ccggtcagga
     2221 ccaggcggtg cacggggacg cccgccaggt cgagatggtg gcggacgacg aagccggcgg
     2281 cctcgaaggc ggcccggcgc agggcggcgg gaccctgggt caggtcgagg ccgtcgagcc
     2341 cggagcggcg gtcggggtcg tgcaggggcg tgcgttcacc ccgcacatag ggcgaccaca
     2401 ccgggatccg gtcgggccgg ggatcgtccc cccgggccag gaggtgggtg gcccagttca
     2461 ggaacaggcc gccggcgttg ctggggccgc cgatcagcgt ccggcccggc gaggagtgcg
     2521 ggatggtcca caggcctggg acctcgaccc actcgtcggt caccgcccag gtgatgagcg
     2581 tggtgccgca gatgaccaac acgtcccccg gctcctcggc cccggccacc agttgctcgc
     2641 cgaacgcgtc gatgcccccc ggcccgatgg cggcgccgtc gatctcaccg acgggcgagt
     2701 cgccgttgac gacatcgggt aggcggtcgg cggtcacgcc ggactcggcc agcatcgcct
     2761 cgtcccaacc ctgtccgtcg tagagcgggt aggccgtcat ggcgctgatc gagtccatcg
     2821 ccccccgccc gccgagggcg tggttggcca ccgcctgggc cggccagtac cccgcggcgt
     2881 cgggcgcctc cccgtggcac cagcgcagga accccaacag ctcgccgaca tcgcccggtg
     2941 cccccgtcgg acgagcggca ccccggcccc gggcgtcgcc gtagagcagg cccggggtca
     3001 gggcgtggcc ccgggcgtcg accgcggtca gcgaggggac catggcgacg acgttgaccc
     3061 cgaccgggtg cagtccggac gagacctgcc gccacgcctg ccggacgccg tcccgccagg
     3121 cccggttggc gtcgtgctcg aagcggtcgg ccgccggggt caggatcggg tggggcaccc
     3181 gcacctgggc gagcacgtga ccgtcgtcgt ccaccgccag gcccttgacc gaggtcgtgc
     3241 cgatgtccag gccgaccgtg acgttgctgt cccccatcgg tcgtcagtct ggcctagccg
     3301 cacccctaag ctccgcggcg gtctcaacca ggaggaacgc aatgggtgtg ttggcaacga
     3361 tcgtggagct gggcagcgac gacacggtct acgggatcat gctcctgctc cacatcctct
     3421 ccgccatcgt gggcttcggt ggcgtgcagc tcaacggcct ctacgcccag caggcccagc
     3481 agcggccggg c
//
LOCUS       DASTFV010000594         2344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2263870, whole genome shotgun sequence.
ACCESSION   DASTFV010000594 DASTFV010000000
VERSION     DASTFV010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2263870"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2153
                     /locus_tag="VFP02_10845"
     CDS             <1..2153
                     /locus_tag="VFP02_10845"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET9543573.1"
                     /translation="KRLNGLDGVAATVNFVTETASIEAPNGVTTDDLVAEVEAVGYGA
                     RLSTRMSATTPAEDGDALRPVREGLVFIGLLTLPVVAMSMVPALQFDDWQWLALALAA
                     PVVAWGGWPFHVAAWANLRHGAATMDTLISIGTLAAFGWSLYALFLGDAGVTGMRMTV
                     DLGLQRDGGAGQIYLEVATGVTFFILLGRYLEARAKQRSGAALRALLDLGAKEVSVLR
                     DGAEMRRPVEDLVVDDRFVVRPGETVAADGVVEDGVSAIDASLLTGESVPVEVGPDAA
                     VTGGTVNVGGRLVVRATRVGADTALARIGRLVTDAQSGKAPVQRLADRVASVFVPIVI
                     GLSLATLAFWLAVGESAEAAFSAAVAVLIIACPCALGLATPTALLVGTGRGAQLGILI
                     KGPDVLESTRRVDTIVLDKTGTVTSGVMALVHVVTTPETDRVEALRLVGALEGASEHP
                     VGRAIADGAEAEVGALPAVVDFHSTAGLGVRGTVDGRSVVVGRSDHVLDAGTTVPDQL
                     VRAQEEAEAAGRTSVLAGWDGSIRAVLVVADTVKPSSAAAVRALRELGLHPVLLTGDN
                     DRAARTVADAVGIDEAVAGVLPEGKVAAVRRLQSEGRVVAMVGDGVNDAAALAQADLG
                     LAMGTGTDVAIEAGDITLVRGDLQSAADAIRLSRRTLRTIKGNLFWAFAYNVVGLPLA
                     AAGLLDPLLAGAAMAASSVFVVSNSLRLRRFAPS"
BASE COUNT          295 a          843 c          866 g          340 t
ORIGIN      
        1 agaagcgact caacggcctc gacggcgtgg ccgccaccgt caacttcgtc accgagacgg
       61 cctccatcga agcccccaac ggcgtgacca ccgacgacct ggtcgccgag gtcgaggccg
      121 tcgggtacgg ggcccgcctg tcgacccgaa tgtccgccac cacgccggcc gaggacggcg
      181 acgcccttcg gcccgtgcgg gaggggctgg tcttcatcgg cctgctgacc ctgccggtgg
      241 tggccatgtc catggtcccg gccctccagt tcgacgactg gcagtggctg gctctggcct
      301 tggccgcgcc ggtcgtggcc tggggcggat ggccgttcca cgtggcggcg tgggccaacc
      361 tccgccacgg cgcggcgacc atggacaccc tcatctcgat cggcacgctg gccgccttcg
      421 gctggtcgct ctacgccctg ttcctggggg acgcgggggt gaccggcatg cgcatgacgg
      481 tggacctggg tctccagcgc gacggtggcg cggggcagat ctacctcgag gtggccaccg
      541 gcgtaacctt cttcatcctc ctggggcgct acctggaggc ccgggccaag cagcgctcgg
      601 gggccgccct ccgggccctg ctcgatctcg gggccaagga ggtcagcgtg ctgcgtgacg
      661 gggccgaaat gcgtcggccg gtcgaggacc tcgtggtcga cgaccgcttc gtggtacgtc
      721 cgggcgagac ggtggccgcc gacggcgtgg tcgaggacgg cgtctccgcc atcgacgcct
      781 cgctgctcac cggcgagagc gtccccgtcg aggtcgggcc ggacgccgcg gtcaccggtg
      841 gcacggtcaa cgtgggcggc cgcctggtcg tccgggccac ccgggtcggg gccgacaccg
      901 ccctggcccg gatcggccgc ctggtgaccg acgcccaaag cggcaaggcg cccgtgcaac
      961 gactggccga ccgggtggcg tcggtcttcg tgccgatcgt catcggcctg tccctggcca
     1021 cgctggcctt ctggctggcg gtcggcgagt ccgccgaggc cgcgttcagc gccgccgtgg
     1081 ccgtcctgat catcgcctgc ccgtgcgcgc tcggcttggc cacgccgacc gccctcctgg
     1141 tcggcaccgg tcggggggcc cagctgggca tcctcatcaa gggtcccgac gtgttggagt
     1201 cgacccgacg cgtcgacacg atcgtcctgg acaagaccgg caccgtgacc agcggtgtga
     1261 tggccctggt ccatgtcgtg accacgccgg agactgatcg ggtcgaggcc ctgcgcctcg
     1321 tcggcgccct ggaaggggcc agcgagcacc cggtgggccg ggccatcgcc gacggggccg
     1381 aggcggaggt cggcgcgctg cccgctgtcg tcgacttcca cagcaccgcg ggcctcggtg
     1441 tccgcggcac cgtcgacggg cggtcggtcg tggtcgggcg gtcggaccac gtgctcgacg
     1501 ccgggaccac ggtgccggac cagttggtcc gggcccagga ggaggcggag gcggcggggc
     1561 ggacgtccgt actcgccggt tgggacggct cgatccgggc cgtgctcgtc gtggccgaca
     1621 cggtgaagcc gtcctcggct gccgccgtcc gtgccctccg cgagctgggc ctgcacccgg
     1681 tcctcctcac cggtgacaac gaccgcgccg ctcgcacggt ggccgatgcc gtcggcatcg
     1741 acgaggcggt ggctggtgtc ctgcccgagg gcaaggtcgc cgccgtccgt cggctccagt
     1801 ccgagggccg cgtggtggcc atggtgggcg acggcgtgaa cgacgccgcg gcactggccc
     1861 aggccgacct cggcctggcc atgggcacgg gcaccgacgt ggccatcgag gccggggaca
     1921 tcacgctggt gcgaggagac ctgcagtcgg cggccgatgc catccggctg tcccggcgca
     1981 ccctgcgcac gatcaagggc aacctcttct gggccttcgc ctacaacgtg gtcggcctgc
     2041 ccctcgccgc cgccggactg ctcgaccccc tcctggccgg cgccgccatg gccgcctcca
     2101 gcgtgttcgt cgtgtccaac agcctgcggc tccgccgctt cgcaccatca tgacgctgcg
     2161 gagtcggagc gaagcagctc gcaagaggtt cgtcggaagc tgccgtaggc agcaggagcc
     2221 gcgcaggcgg cgacaggagg aacagccttc acatgcccgg gtatgagatg gagaaggacc
     2281 agatcgcggc tcgactggcg cgcatcgagg gccaggtgcg agggatcgcc cgcatggtcg
     2341 acga
//
LOCUS       DASTFV010000595         2957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2267249, whole genome shotgun sequence.
ACCESSION   DASTFV010000595 DASTFV010000000
VERSION     DASTFV010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2267249"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VFP02_10850"
     CDS             <1..709
                     /locus_tag="VFP02_10850"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HET9543574.1"
                     /translation="ESGPSDGSRLPEHGRLQMLAVAGHPNADLAASQPQGARFRVRWV
                     DIDDPDPTFPHTPGEPAPTTNDQALRHVGDQGRAQGAAWFSRLEGSVYDDGTVWFTST
                     QGGGAPETSIGPIANGFGNGSGQVWAYDTRSQMLRMAYESPGPETLDLPDNVTRRSRN
                     GALVLCEDGTNDNYLRGLTRRGELFDIALNRLPGQFNDEFAGATFSPDGHTLFVNIQA
                     SRALTFAIWGPWDRLGV"
     gene            complement(727..1593)
                     /locus_tag="VFP02_10855"
     CDS             complement(727..1593)
                     /locus_tag="VFP02_10855"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9543575.1"
                     /translation="MTATTVTDVSAVNPARPHERVLPEPRGVFHDTWVVAKRGLIHMK
                     RQPEALSDATVQPIMFVLLFGFVFGGAIGVPGGSYREFLMGGIFAQTIVFTAFGVAMA
                     IANDFKNQAVDRFRSLPIARGAVLGGHAVANLAKTVLPIVIMSVCGLLVGWRIDGGLL
                     DAIAGYGLMLVFAFAMIWVGVLLGSVVGTPEGVTGLGFVALFPLTFLASTFVPVNTMP
                     PALQTVAEWNPVSTLSDAIRVLFDNPRPPVTPGDPWPSHHPVAYTLLWAIAIVAVCAP
                     LAVRAYDRSIAR"
     gene            complement(1590..2570)
                     /locus_tag="VFP02_10860"
     CDS             complement(1590..2570)
                     /locus_tag="VFP02_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9543576.1"
                     /translation="MPEANPSFAVRARGLVKRYGETTALAGIDFEVPTGTVTAVLGPN
                     GAGKTTAVRILTTLADADEGEAVVAGFDVREDPKEVRRRIGLAAQDATVDPLLTGREN
                     LIMLGELHQYRRKEAVIRAKALLEEFNLADAADRRTSGYSGGMRRRLDLAATLVSDPE
                     VLFLDEPTTGLDPRARAELWSVLDHLVDRGTTLLLTTQYLDEAERLADDIIVIDHGRI
                     IARGDARKLKREVGGDHLQVVVVDPSRLDDAVRLVAEVTGLEPRVEVGARRVNAPISQ
                     GVDALVEAAKALAVAGIAVDDLSLRQPTLDEVFLDLTGAAPEETDEEDQS"
     gene            complement(2716..>2957)
                     /locus_tag="VFP02_10865"
     CDS             complement(2716..>2957)
                     /locus_tag="VFP02_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9543577.1"
                     /translation="IDDVAARADRVVARIVGVQGDIAVFSHGHFLRVLAARWLGLAPT
                     AGGMLLLDTGTISVLSTERETPAIRLWNGVPPSTT"
BASE COUNT          447 a         1056 c         1060 g          394 t
ORIGIN      
        1 cgaatccgga ccctccgacg gctcgcgcct gccggagcac ggccggctcc agatgctggc
       61 tgtggccggg cacccgaacg ccgacctcgc cgccagccag ccccaggggg cgcggttccg
      121 ggtgcggtgg gtcgacatcg acgacccgga cccgaccttc ccccacaccc ccggggagcc
      181 cgccccgacg accaacgacc aggcccttcg tcacgtgggc gaccagggcc gggcccaggg
      241 ggcggcgtgg ttctcccggc tcgaagggtc cgtgtacgac gacggcacgg tctggttcac
      301 gtcgacccag gggggcgggg cccccgagac gagcatcggg cccatcgcca acggcttcgg
      361 caacggatcg ggccaggtct gggcctacga cacccgcagc cagatgctgc ggatggcgta
      421 cgagtccccc ggcccggaga ccctggacct ccccgacaac gtcacccggc gtagccgcaa
      481 tggcgccctg gtcctctgcg aggacggcac caacgacaac tacctgcggg ggctcacccg
      541 ccgcggcgag ctcttcgaca tcgccctcaa ccggctaccc ggtcagttca acgacgagtt
      601 cgccggggcc acgttcagcc cggacggcca caccctgttc gtgaacatcc aggccagccg
      661 ggccctgacc ttcgccatct ggggaccctg ggaccgcctc ggcgtctgag cccggggccg
      721 gcccgctcag cgggcgatgg agcggtcgta ggcgcgcacg gcgagcgggg cgcagacggc
      781 cacgatcgca atggcccaga gcagggtgta ggccaccggg tggtggctcg gccacgggtc
      841 gcccggcgtc acgggtgggc gagggttgtc gaacagcacg cggatggcgt ccgacagggt
      901 cgacaccggg ttccattcgg cgacggtctg cagggccggg ggcatcgtgt tgaccggcac
      961 gaaggtgctg gccaggaagg tcagcgggaa cagggccacg aaaccgagcc cggtgacccc
     1021 ttccggcgtg ccgaccacgc tgccgagcag cacaccgacc cagatcatgg cgaaggcgaa
     1081 caccagcatc aggccgtagc cggcgatcgc gtccagcagg ccgccgtcga tgcgccaccc
     1141 cacgagcagg ccgcagaccg acatgatcac gatcggcagc accgtcttgg ccaggttggc
     1201 cacagcgtgg ccccccagca cggcgccgcg cgcgatcggc agggaccgga agcggtcgac
     1261 ggcctggttc ttgaagtcgt tggcgatcgc catcgccacg ccgaaggcgg tgaagacgat
     1321 ggtctgggcg aagatcccgc ccatgaggaa ctcccggtag ctcccacccg ggacgccgat
     1381 ggcgccgccg aagacgaagc cgaagagcag cacgaacatg atgggctgga ccgtggcgtc
     1441 gctcagggcc tcgggctggc gcttcatgtg gatgaggccc cgcttggcga cgacccacgt
     1501 gtcgtggaac acgcccctgg gctcgggcag cacgcgctcg tggggtcgcg cggggttcac
     1561 ggcgctgaca tcggtgacgg tggtggcggt cacgactggt cctcctcgtc ggtctcctcg
     1621 ggggcggccc cggtgaggtc caggaacacc tcgtccaggg tcggctggcg caggctcaga
     1681 tcgtcgacgg cgatccccgc caccgccagc gccttggccg cctcgaccag cgcgtccacg
     1741 ccctgactga tgggggcatt gacgcggcgg gcaccgacct caacccgggg ctcgagcccg
     1801 gtcacctcgg ccaccaggcg gacggcgtcg tccagccggg aaggatcgac gaccaccacc
     1861 tgcaggtggt cgcctccgac ctcccgcttg agcttgcggg cgtcgccccg ggcgatgatc
     1921 cggccgtggt cgatgacgat gatgtcgtcg gccaggcgct cggcttcgtc caggtactgg
     1981 gtggtgagca gcaacgtcgt gccccggtcc acgaggtggt cgaggaccga ccacagctcg
     2041 gcgcgggccc gcgggtccag cccggtcgtg ggctcgtcga ggaagaggac ttcggggtcc
     2101 gacaccagcg tggcggcgag atcgaggcgc cggcgcatgc cgccggagta gcccgaggtc
     2161 cggcggtcgg cggcgtcggc caggttgaac tcctccagca gggccttggc ccggatgacg
     2221 gcctccttgc gccgatactg gtggagctca cccagcatga tcaggttctc ccggccggtc
     2281 agcagcgggt cgaccgtggc gtcctgggcg gccaagccga tccggcgccg gacctccttg
     2341 gggtcctccc tgacgtcgaa cccggcgacg acagcctcgc cctcatcggc gtcggccagg
     2401 gtggtgagaa tgcgcaccgc ggtggtcttg cccgccccgt tggggccgag cacggcggtg
     2461 acggtcccgg ttgggacctc gaagtcgatc ccggccaggg ccgtggtctc gccgtagcgc
     2521 ttcaccagcc cgcgggcgcg gacggcgaac gacgggttgg cctccggcat cgtcaccctc
     2581 ctcggaatct cggtcacggt catagacagt tggaccaccg aacccggcga gactcatcgc
     2641 cggccttccg cggtcccgat cacgaccgta ttggccccgc ccggattcac gaacgccttt
     2701 ccggcgtgga acctgtcacg tcgtggaggg tgggacaccg ttccagagcc ggatggccgg
     2761 ggtctcccgc tccgtgctga gcacgctgat cgtcccggtg tcgagcagca gcatcccgcc
     2821 ggcggtcggg gccaggccga gccaacgggc ggccaggacc cgcaggaagt ggccgtggga
     2881 gaagacggcg atgtcgccct gcacgccgac gatccgggcg accacccggt cggcccgggc
     2941 ggcgacgtcg tcgatcg
//
LOCUS       DASTFV010000596         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2267522, whole genome shotgun sequence.
ACCESSION   DASTFV010000596 DASTFV010000000
VERSION     DASTFV010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2267522"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFP02_10870"
     CDS             complement(<1..731)
                     /locus_tag="VFP02_10870"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET9543578.1"
                     /translation="MLGVVTVVALSGLGGLHAAAQSSGDQPALADDPELVAAGQELFA
                     TGCSSCHGLAGEGSVNGPRIADAGEAAADFQLRTGRMPAADVEGQQPGKDPAYGDHEI
                     QALVAYVGSLGDGPRIPDVDPARGDLANGGELYRANCAACHNASGIGGALSYGGEAPS
                     VRDIGPVQVGEAMRTGPGQMPVFGPDTFSPQEVDDIARYVQELAEPSRRGGLSLGSVG
                     PVTEGFVAVLFALGSIVLATRWITAR"
     gene            complement(740..1333)
                     /locus_tag="VFP02_10875"
     CDS             complement(740..1333)
                     /locus_tag="VFP02_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET9543579.1"
                     /translation="MAPPDTAVAVDGAPVEGAPLANPLPVGVIVWLSSELMFFAGLFA
                     AYFTLRSANPVWPSAAADLDEVRAAIFTTVLVVSSLTMHRSVRAAEADRREEAMRWLA
                     ATFLLGLVFVVNQALEYAHLGFGIDTDAYGSIFWLLTGFHWLHVVAGLILMLALARLS
                     WAGTRAPVEVHHQVVGYYWHFVDVVWVAVFAAVYLIQ"
     gene            1407..>2227
                     /locus_tag="VFP02_10880"
     CDS             1407..>2227
                     /locus_tag="VFP02_10880"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET9543580.1"
                     /translation="MPLPPLSPGAMVTEMAFDPLGASVVVLGVVGYAVGVRRLADRGR
                     AWPVGRSAAFAAALVAFSVATLSGLAAYDTVQFSAHMAQHLLLGMIVPVLLVVARPVT
                     LILQAGSRATQVTTLRVLHHPVVAFLTEPIVAFVAFVSTLWLLYFTPLYDLSTRNDAV
                     HLGLHVHFVVVGVLFYGAVLATDPRPHAAPPATRVGLAFLALPAHAFLGITLLAGTAI
                     IGPAAHLVGRRPWQGDALADQRLAAGLLLSVGEIVGLVVVLAIVWSWMATEGRAA"
BASE COUNT          294 a          796 c          813 g          324 t
ORIGIN      
        1 cgggcggtga tccatcgggt ggcgaggacg atgctgccga gggcgaagag gacggcgacg
       61 aacccttccg tcaccggtcc gacgctgccc aggctcaatc cgccccggcg cgacggctca
      121 gccagctctt ggacgtaccg ggcgatgtcg tccacctctt ggggcgagaa cgtgtcgggc
      181 ccgaacacgg gcatctggcc gggaccggtg cgcatcgcct cgcccacctg gaccggcccg
      241 atgtcgcgga cgctgggtgc ctcaccgccg tagctcagcg ccccaccgat gccgctggcg
      301 ttgtgacagg ccgcgcagtt ggcccggtag agctcgccgc cgttggccag gtcgccccgg
      361 gccgggtcca cgtcggggat gcgggggccg tcgcccaggg agccgacata ggcgaccagc
      421 gcctggatct cgtgatcgcc gtacgcgggg tccttccccg gttgctggcc ctcgacgtcg
      481 gcggcgggca tgcgcccggt cctgagctgg aagtcggccg ccgcctcccc tgcgtcggcg
      541 atgcggggac cgttgacgct gccctcgccg gccagcccgt ggcagctcga gcagcccgtg
      601 gcgaagagct cttggccggc cgcgaccagc tcggggtcgt cggccagtgc gggctggtcg
      661 ccgctcgact gggcggcggc gtgcaggccg cccaacccgc tcagcgccac caccgtcacc
      721 acgccgagca ccgaccccct cactggatca ggtagaccgc ggcgaagacg gcgacccaga
      781 cgacgtcgac gaagtgccag tagtagccca cgacctggtg gtggacctcg acgggcgccc
      841 gcgtgccggc ccagctgagc cgggccaagg cgagcatcag gatgaggccg gcgacgacgt
      901 ggagccagtg gaagccggtc agcaaccaga agatcgatcc gtaggcatcg gtgtcgatgc
      961 cgaagccgag gtgcgcgtac tcgagggcct ggttcacgac gaacacgagg ccgagcagga
     1021 aggtggcggc gagccagcgc atcgcctcct cccgacggtc ggcttcggcc gcccggaccg
     1081 accggtgcat ggtcaggctg ctgaccacca ggacggtggt gaagatggcg gcccgcacct
     1141 cgtcgaggtc agccgcggcg gacggccaga cggggttggc cgatcggagg gtgaagtagg
     1201 cggcgaacag cccggcgaag aacatgagct cggagctgag ccagacgatc acgcccacgg
     1261 gcagcgggtt ggccaggggg gcaccctcga ccggtgcccc gtcgaccgcc acggcggtgt
     1321 ccggtggggc cacgggaact tcctaccacg gcgacacccc gggcgagcgt ccggtggccc
     1381 ggggggtgcg gggttagggt cgacaggtgc cgctgccgcc gctgtcaccg ggcgccatgg
     1441 tcaccgagat ggccttcgat ccgctcgggg cgagcgtggt cgtgctcggt gtcgtggggt
     1501 acgcggtagg ggtgcggcgc ttggccgacc ggggacgggc atggccggtg gggcgctcgg
     1561 ccgccttcgc cgcagccctc gtcgccttca gcgtcgccac gctgtcaggc ctggcggcct
     1621 acgacacggt ccagttctcg gcccacatgg cccagcacct cctgctgggg atgatcgtcc
     1681 ccgtcctgtt ggtcgtcgcc cggccggtga ccctgatcct ccaggccggt tcacgagcga
     1741 cccaggtgac gacgctgcgg gtcctgcacc acccggtcgt ggccttcctg accgagccga
     1801 tcgtggcgtt cgtcgccttc gtgtcgacgc tctggctgct ctacttcacg ccgctgtacg
     1861 acctcagcac ccggaacgac gcggtccacc tcggcctgca cgtgcacttc gtcgtcgtcg
     1921 gcgtgctgtt ctacggcgcc gtgctggcca ccgaccctcg cccgcatgcg gcgccgccgg
     1981 cgactcgggt cggcctggcc ttcctggcgc tgccggccca cgcgttcctc ggtatcaccc
     2041 tgctggcggg aacggcgatc atcggtcccg ccgcccacct cgtcgggcgc cgaccgtggc
     2101 agggagacgc cctggccgat cagcgcctgg cggccggcct cctcctctcg gtgggcgaga
     2161 tcgtcggact ggtggtggtc ctggccatcg tctggtcgtg gatggccacc gaaggccgcg
     2221 ccgctga
//
LOCUS       DASTFV010000597         3480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2270428, whole genome shotgun sequence.
ACCESSION   DASTFV010000597 DASTFV010000000
VERSION     DASTFV010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2270428"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFP02_10885"
     CDS             complement(<1..479)
                     /locus_tag="VFP02_10885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9543581.1"
                     /translation="MTGVRRDSPEGRWLLAAAVLGSGLAALDATVINVALPAIGEDLD
                     TDITGLQWILNGYLLTLASFLLLGGSLGDRYGRRRVFVIGVVWFAGASLLCGLAPSVE
                     VMVAARALQGVGGALLTPGSLALLQSGFHPDDRARAIGAWSGLGGVATAIGPFAGGW"
     gene            complement(476..1480)
                     /locus_tag="VFP02_10890"
     CDS             complement(476..1480)
                     /locus_tag="VFP02_10890"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9543582.1"
                     /translation="MTEGARTSEAERQALLDLATRALLDGERRYTALEVCALTGCDHE
                     TADGLWRAMGFPNVPDEEIAFTDRDVAALRAALDLQTSGLLDEATVRGQTRVMSQALA
                     TIAAAHLEITEPGERNVDRLGAFVADVLPNLDDLLVYLYRRHLLAALEGSLLLDRDDP
                     EQLNPTLTVGFADLVGFTKLANHLEEDELSQLIEGFTQAAADVVAEEAGRVVKMIGDE
                     VMFTSADPSAAATTALRLVAEIGDHDGLPPLRAGVAAGPVILRHGDVFGNPVNLAHRL
                     VDVARPGNVLVDEAVAGAVGPLHGEIQRVHSIRRLRGFDKVRVFVLRPLQPAIRTDVE
                     "
     gene            1577..2005
                     /locus_tag="VFP02_10895"
     CDS             1577..2005
                     /locus_tag="VFP02_10895"
                     /EC_number="3.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF020622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A2"
                     /protein_id="HET9543583.1"
                     /translation="MLCPKRCGAFVLTTVALTITLGLLAPNAAGATTGHGAVATPANL
                     TPHPWPYDGCSAAPERGPGWDFHHACIHHDGCYRGHWASRRTCDGWFLRDMRASCLVL
                     HPSGVLARWACDGIAAVYYSAVRLFGSAAYAHRRVDVPVR"
     gene            complement(2354..2485)
                     /locus_tag="VFP02_10900"
     CDS             complement(2354..2485)
                     /locus_tag="VFP02_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543584.1"
                     /translation="MSFRDHVIAALLAIAVMAASLMISHAPAETPATVAVAADQDRR"
     gene            complement(2571..3326)
                     /locus_tag="VFP02_10905"
     CDS             complement(2571..3326)
                     /locus_tag="VFP02_10905"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9543585.1"
                     /translation="MSNGNGRLDGRIAFVTGAASGIGAACAQRFVDEGARVAGFDLQD
                     PAEGSPLAGFWSLDVRDEPAITSAVAAAVAEVGVPTVLVNAAGVSGTASVTDLAEEEW
                     DRIVDINLKGTYLVSKHVVPHLVAAGRGSVVNLASIEGLEGLPGQAAYNASKGGVVLL
                     TRNMAIDYGPANVRVNCLCPGYIETPMTAVLQAPGLEAIREEFIAMHQLGRAGQPEEV
                     AAAALFLASDDASFVSGHALVVDGGFTAGRRLG"
BASE COUNT          481 a         1226 c         1321 g          452 t
ORIGIN      
        1 aaccagccgc cggcgaaggg gccgatggcg gtggccacgc cgccgaggcc cgaccaggcc
       61 ccgatcgccc gggcccggtc gtcgggatgg aacccggact ggaggagggc gaggctgccg
      121 ggggtcagca aggcgccgcc gacgccctgg agggcgcggg cggcgaccat gacctccacg
      181 gagggagcga gcccgcacag cagggacgcg ccggcgaacc acacgacgcc gatgacgaag
      241 acccggcggc ggccgtagcg gtcacccagc gacccgccga ggaggaggaa cgacgccagg
      301 gtcagcaggt agccgttgag gatccactgc aggccggtga tgtcggtgtc caggtcctcg
      361 ccgatggcgg ggagcgccac gttgatgacc gtggcgtcga gggcagcgag gcctgagccg
      421 aggacggcgg ccgccagcaa ccatcgccct tcgggcgagt cccgccggac gccggtcact
      481 cgacgtcggt gcggatggcg ggctggagtg ggcggagcac gaagacccgg accttgtcga
      541 agccgcggag ccggcggatg gagtgcaccc gctggatctc gccgtggagc gggcccacgg
      601 cgccggccac ggcctcgtcg accaggacgt tgccgggacg ggccacgtcc accagccggt
      661 gggccaggtt caccgggttc ccgaacacgt cgccgtgacg caggatcacg ggcccggcgg
      721 cgacaccggc ccgcagcggg ggcaggccgt cgtggtcacc gatctcggcc accaggcgca
      781 gggccgtcgt tgccgccgcg ctcgggtcgg ccgaggtgaa catgacctcg tcgccgatca
      841 tcttcaccac ccgcccggcc tcctcggcca ccacgtcggc cgcggcctgg gtgaagccct
      901 cgatgagttg ggacaactcg tcctcctcga ggtggttggc cagcttggtg aagcccacga
      961 gatcggcgaa gccgacggtc agggtggggt tgagctgttc gggatcgtcc cgatcgagca
     1021 gcagcgagcc ctcgagcgcg gccaggaggt gccgccggta gaggtagacg aggaggtcgt
     1081 ccaagttggg caggacgtcg gccacgaagg caccgaggcg gtccacgttg cgttcgcccg
     1141 gctcggtgat ctccaggtgg gcggcggcga tggtggccag ggcctggctc atgacccggg
     1201 tctggccccg cacggtcgcc tcgtcgagca ggccgctggt ctgcaggtcg agggcggccc
     1261 ggagggcggc tacgtcccgg tcggtgaagg cgatctcttc gtcgggtacg ttcgggaagc
     1321 ccatggcccg ccagagcccg tcggcggtct cgtggtcaca gcccgtcagg gcacagacct
     1381 cgagggccgt gtagcggcgc tcgccgtcga gcagggcccg cgtcgccagg tcgagcagtg
     1441 cctggcgctc ggcctcgctg gtcctcgccc cctcggtcac gggcgaacgc taccggcccc
     1501 ttgcgagggg cttcggggcc gggcaggatc ggggggacag cgcttccccc tcatcaccct
     1561 ccgaacgggg aagctcatgc tgtgtccgaa gcggtgcggc gcgttcgtcc tgacgaccgt
     1621 cgccctgacc atcaccctgg ggctcctggc gccgaacgcg gccggggcca cgaccggtca
     1681 cggtgcggtg gccacaccgg ccaacctcac cccgcacccc tggccctacg acggttgctc
     1741 ggccgcaccc gagcgaggtc ccgggtggga cttccaccac gcctgcatcc accacgacgg
     1801 ctgctaccgc ggccactggg ccagccgccg cacctgtgac ggctggttcc tccgggacat
     1861 gcgagcgtcg tgcctggtcc tgcacccgag cggcgtcctg gcccggtggg cctgcgacgg
     1921 catcgccgcc gtctactact cggcggtgcg gctgttcggg tctgcggcct acgcgcaccg
     1981 ccgggtggac gtccccgtgc gctgaagccc ttcggggccc ggccctcgac gggccgggcc
     2041 ccgcgccggg ggtgggcgga ggaaggctca gccggatgcg acagcgagcg gaggggtcac
     2101 cggtgctccg gccgtcggac aggcggaagg tgacgatggg gtcgtgcccc agccggttca
     2161 gggccacgag gcgatcgcgg acgaacgggg tcgccgggtc gtcggcaccg cggtcggtca
     2221 caccggtcag gtcgactgtc tccggcgagc gcgagcccca gcggcggtgc ggtggggccg
     2281 gctcccgctc tcgtggcggc gagtcccagc gccgcgatcg acaggaaggc ggcgacgacc
     2341 aggcggcgcc gcatcagcgt cggtcctggt ccgcggcgac ggcgacggtc gccggcgtct
     2401 cggcgggggc atggctgatc atgaggctgg cggccatcac cgcgatggcc agcagagcgg
     2461 cgatcacatg gtctcggaag ctcatggcgc gcacggtagg gagcggcggt gacgcccctg
     2521 gtgcgctcgg gtgaccgccg ggtgacggtt gggcgaaggc tgtgggtggc tcagcccagg
     2581 cgccggccgg cggtgaaccc accgtccacg acgagggcgt gaccgctgac gaacgaggcg
     2641 tcgtccgagg ccaagaagag ggcggcggcg gccacctctt cgggctgacc ggcccgcccc
     2701 agctggtgca tggcgatgaa ctcctcccgg atcgcctcca ggccgggggc ctgcagcacc
     2761 gcggtcattg gcgtctcgat gtagcccggg cagaggcagt tgacccggac gttggccggc
     2821 ccgtagtcga tggccatgtt ccgggtcagc aggaccaccc cgcccttgga ggcgttgtag
     2881 gcggcctggc cgggcaggcc ctccaggccc tcgatgctgg ccaggttgac cacagacccc
     2941 cggcccgcgg cgaccaaatg gggcaccacg tgcttgctga ccagataggt gcccttgagg
     3001 ttgatgtcga cgatgcggtc ccactcctcc tcggccaggt cggtgacgct cgccgtcccc
     3061 gacacgccgg cggcattgac caggaccgtg gggacgccga cctcggcgac ggcggcggcg
     3121 accgccgagg tgatggcggg ctcgtcgcgg acgtcgaggg accagaagcc ggccaggggg
     3181 ctcccttcgg ccgggtcctg gaggtcgaag ccggccaccc tggccccctc gtccacgaag
     3241 cgctgtgcac acgccgcccc gatgcccgag gccgccccgg tgacgaaggc gatccgtccg
     3301 tccagccgac cgttgccgtt gctcatctca caacctcccg gcgtccgtga cgcgcttcag
     3361 gaactgtcgc gtccgctcct cgcggggctc ggcgaacagg gccgcgggcg ggccctcctc
     3421 gaggacccgg ccctcgtgca tgaaggccac gcggtgggcc acgtcgcggg cgaagcccat
//
LOCUS       DASTFV010000598         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2278540, whole genome shotgun sequence.
ACCESSION   DASTFV010000598 DASTFV010000000
VERSION     DASTFV010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2278540"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VFP02_10910"
     CDS             <1..782
                     /locus_tag="VFP02_10910"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET9543586.1"
                     /translation="PALPEIEPCGPGASLAEQLAVQGFALVPGVFSPDEVAMLRAEVT
                     RLTEAARPDDHRSWWARTGDGDDVCCRLLYTDDGSDLITEVVTGDDRLADLVAPTGEN
                     VRLAADRFDGLSVVMKPPGVVEGLADLPWHRDCGLGGHSIMCPEFNVGIQLEAATAET
                     GHLEFLPGSHASGWWPRDPHSVDGRTVVAVDTEPGDVTIHYGHVLHRAGPPTGVGGRR
                     ALYATFVQDHAFDYVGPRQTINDVLFASADGGILDDRGTAG"
     gene            complement(789..1613)
                     /locus_tag="VFP02_10915"
     CDS             complement(789..1613)
                     /locus_tag="VFP02_10915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9543587.1"
                     /translation="MSPVALVTGASRGIGRATAIALAEKGFDVALAARTVHEGDGRDD
                     ARPGSAPLPGSLEATASAVEALGVRALPVRMDLHERESLTAGAATVLDVWGHIDVLVN
                     NAVDTGPGSMTTVADTTLDDYQRKLEGNFLAQLVLIHAVLPHLLERGEGTIVDITSAA
                     GITDPPAPAGEGGWGLAYAASKAAFHRVAAHLAVEFGPRGVLAFNVEPGMVMTEKMAV
                     NQKAMGLENRFPVAPPSVPAAVVAWLCTDPEAADHNGETFTAQRFAKDRGLHQPWW"
     gene            complement(1610..2410)
                     /locus_tag="VFP02_10920"
     CDS             complement(1610..2410)
                     /locus_tag="VFP02_10920"
                     /EC_number="1.1.1.194"
                     /inference="COORDINATES: protein motif:HMM:NF009092.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl-alcohol dehydrogenase"
                     /protein_id="HET9543588.1"
                     /translation="MSRPRALDFEGRRVVVTGASSGIGAATAELFDGLGADVVGLDVA
                     DDSLDLREPAAIDAAVAALRGTPVWALVNCAGVPQTAGGRIVLTVNFLGTRYLTEALL
                     PHIVEGGAVASVASLAGQGWPEHVDLLGELLATQTFEDGLAWVDTHVDAMDDPYFFSK
                     EAVILWTRRRARTLRRDHGVRMSTVSPGPVDSPMMPSFREAMDEAVLEWTAREAIGRM
                     ARPDEMALPLVFLCAPGASYVSGIDLIADAGFKTDLDEGLVDFEGLGG"
     gene            complement(2407..>2863)
                     /locus_tag="VFP02_10925"
     CDS             complement(2407..>2863)
                     /locus_tag="VFP02_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9543589.1"
                     /translation="ERVAICNAGLIWRYLEEVTDWAKATKTADGDRVIDEQWVKLGLA
                     RVRASAEVLQLMNWKVAWGLTVGDLSPADASATKVFGTHKYIETLGILMEIMGPISFL
                     RAGSPDALLRGRMERSYQGILVLTFGGGTNEIQRDIIAMLGLGLPRAPR"
BASE COUNT          393 a         1022 c         1044 g          404 t
ORIGIN      
        1 acccggccct gcccgagatc gagccgtgcg ggcccggcgc gtcgttggcc gagcagctcg
       61 ccgtccaggg cttcgctctc gtgcccggtg tgttcagccc ggacgaagtg gccatgctgc
      121 gggccgaggt cacccgcctg accgaggcgg cccggccgga cgaccatcgc tcctggtggg
      181 cccgtaccgg tgatggtgac gacgtctgct gccggctgct ctacaccgac gacggatcgg
      241 acctcatcac cgaggtcgtg accggcgacg accgcctggc cgacctcgtg gccccgaccg
      301 gggagaacgt gcgcctggcc gccgaccgct tcgacggcct gagcgtcgtc atgaagcccc
      361 cgggcgtggt cgagggcctg gccgacctgc cctggcaccg ggactgcgga ctcggcgggc
      421 actcgatcat gtgccccgag ttcaacgtcg gcatccagct cgaggcggcc acggccgaga
      481 ccggccatct cgagttcctc ccgggctcgc acgcctcggg ctggtggccc cgtgaccccc
      541 actccgtcga cggtcggacc gtcgtggccg tcgacaccga gcccggcgac gtgaccatcc
      601 actacggcca cgtcctgcac cgggccggcc cgccgaccgg tgtcggtggc cggcgcgccc
      661 tctatgccac cttcgtccag gaccacgcct tcgactacgt cggcccccgc cagaccatca
      721 acgatgtcct gttcgccagc gccgacggcg gcatcctcga cgaccgcggc accgccggct
      781 gaggcgggtc accaccaggg ttggtggaga ccgcggtctt tggcgaatcg ctgggcggtg
      841 aaggtctcgc cgttgtggtc ggcggcttcg gggtcggtgc acagccaggc cacgacggcc
      901 gccgggaccg agggcggggc gacggggaac ctgttctcca ggcccatggc cttctggttg
      961 acggccatct tctcggtcat gaccatgcct ggctcgacgt tgaaggccag gacgccccgg
     1021 gggccgaact cgacggccaa atgggcggcc actcggtgga aggccgcctt cgaggcggcg
     1081 taggcgagtc cccaaccgcc ctcgccggcg ggggcgggcg ggtcggtgat cccggccgcg
     1141 gaggtgatgt cgacgatcgt gccctcgccc cgctcgagga ggtgggggag cacggcgtgg
     1201 atgaggacga gctgggcgag gaaattaccc tccagcttgc gctggtagtc gtccagggtc
     1261 gtgtcggcga cggtggtcat ggagccgggg ccggtgtcca cggcgttgtt gaccaggacg
     1321 tcgatgtgcc cccagacgtc gagcaccgtc gccgcgcctg cggtcagcga ctctcgctcg
     1381 tggaggtcca tgcgcacggg cagcgcccgg acgccgagcg cttcgaccgc ggacgccgtg
     1441 gcctcgaggc tgcctggcaa tggcgcggaa ccggggcggg cgtcgtcccg gccgtccccc
     1501 tcgtggacgg tgcgggcggc gagggcgacg tcgaagccct tctcggccag cgcgatcgcc
     1561 gtggcccgcc cgatgccccg gctggccccg gtgacgagcg cgaccgggct caaccgccga
     1621 ggccctcgaa gtcgaccaag ccttcgtcca ggtccgtctt gaagccggcg tcggcgatga
     1681 ggtcgatccc cgagacgtag gaagcgcccg gcgcgcacag gaagacgagg ggcagggcca
     1741 tctcgtcggg gcgggccatg cggccgatgg cctcgcgggc cgtccactcg aggacggctt
     1801 cgtccatggc ctcccggaag gacggcatca tcggcgagtc gaccgggccc gggctgaccg
     1861 tgctcatccg cacaccgtgg tcccgccgga gcgtccgggc gcggcgtcgg gtccagagga
     1921 tgacggcctc cttggagaag aagtacgggt catccatggc gtcgacgtgg gtgtcgaccc
     1981 aggccagacc gtcctcgaag gtctgggtgg ccagcagctc gccgaggagg tcgacgtgct
     2041 cgggccagcc ctgcccggcc agggaggcga cgctggcgac cgcgcccccc tccacgatgt
     2101 gcggcagtag cgcctcggtc aggtagcggg tcccgaggaa gttgaccgtc aggacgatcc
     2161 gcccgcccgc cgtctgcggc actcccgcgc agttgaccag tgcccagacc ggcgtgcccc
     2221 ggagagcggc gaccgcggcg tcgatggccg cgggttcccg caggtcgagc gagtcgtcgg
     2281 ccacgtcgag cccgaccacg tcggccccga ggccgtcgaa gagctcggcc gtggcggcgc
     2341 cgatgccgga cgaagcgccg gtgacgacga cccgccggcc ctcgaagtcc aacgcgcggg
     2401 gccggctcac cgcggcgcgc ggggcaggcc gaggccgagc atggcgatga tgtcccgctg
     2461 gatctcgttg gtcccgccac cgaaggtcag gaccaggatc ccctggtagc tgcgctccat
     2521 ccgcccgcgg agcagggcgt cgggcgaacc ggcccgcagg aaggagatgg ggcccatgat
     2581 ctccatgagg atccccaggg tctcgatgta cttgtgggtg ccgaagacct tggtggcgga
     2641 cgcgtcggcc gggctgaggt caccgaccgt cagcccccat gccaccttcc agttcatgag
     2701 ctggagcacc tcggcggagg cccgcacccg ggccaggccg agcttcaccc actgttcgtc
     2761 gatgacccgg tcaccatcgg ccgtcttggt ggccttggcc cagtcggtga cctcctccag
     2821 gtagcgccag atgaggccgg cgttgcagat ggccactcgc tcg
//
LOCUS       DASTFV010000599         4444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2281072, whole genome shotgun sequence.
ACCESSION   DASTFV010000599 DASTFV010000000
VERSION     DASTFV010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2281072"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VFP02_10930"
     CDS             complement(<1..470)
                     /locus_tag="VFP02_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9543590.1"
                     /translation="MPDLLARTEELCAIPSLSRDEATITDHLDGLLAAVPGLERTRVG
                     DNLVARTNLGRPLRLVLAGHTDTVPANGNAAPRREGTTLWGLGTADMKGGLAVMVELA
                     LAVPEPAVDVTYVFYAREEIAAKESGLEELFVERPDLLEGDVAILGEPTDGEVEA"
     gene            complement(470..1294)
                     /locus_tag="VFP02_10935"
     CDS             complement(470..1294)
                     /locus_tag="VFP02_10935"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:NF008808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HET9543591.1"
                     /translation="MSDLQASIDDLWARRDDLGPGDDDARRIVHEAVDLLDSGAARVA
                     EWDGEGAVTVNEWLKRAVLLLFRVQGMETIELGPFEYADKLPLKSDYEAKGVRVVPGA
                     SARWGSFLDRGVVMMPSYVNIGAHVASGSMVDTWATVGSCAQIGRNVHLSGGVGIGGV
                     LEPVQAAPVMIGDECYIGSRCIVAEGATVGTGTVLGAGSILTASVPVIDAESGAEISR
                     GHVPPWSVAVTATRPRAFAGGTFGLPCVLVIKRLTEGERHDKGALNDILRDHGSTV"
     gene            complement(1329..2060)
                     /locus_tag="VFP02_10940"
     CDS             complement(1329..2060)
                     /locus_tag="VFP02_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543592.1"
                     /translation="MTSPFGADGAYPMDQPQGPSSLGYWLGGLLIAGGIIGGIVWGWV
                     SFSSFLDAIDDFERVPVGEISTIQLDDGDYVVYAERGGGDAVSLFVDDVRMRPAGQEG
                     ADEIEFEPYVSEFTYDFGNHTGRAQLTFEIEETGEYDVRVGGGPATTATTAAFGPSVA
                     SDLVASIVGGFVIAGIGIVLGIVLVIVTGVRRRKFRQRAWLDARNGPGGAPQPGPWNP
                     PPPASPGAWSGNPPPPAGYPPPPTA"
     gene            complement(2057..3169)
                     /locus_tag="VFP02_10945"
     CDS             complement(2057..3169)
                     /locus_tag="VFP02_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9543593.1"
                     /translation="MTEGFQPPPYPYDRLDELKAVADALPGGCVDLSIGTPNDPPPAA
                     VVEALAGSGAERGYPPSIGSAPYRRAAAGWLARRLGVTVDPDSLAACVGTKEFVVGTP
                     SFLKLRAPDRDTVLHPQVAYPSYAMGATLARCRSVAVPPSPDGTLDLDAVSAADAERA
                     LCLWVNSPGNPAGRLDDLGSAAAWGRAHGVPVLSDECYVEFTWDGPPRSILEHGIEGV
                     LAVHSLSKRSNLAGVRAGFYAGDPELVTYLREVRKHAGMMVPGPVQAAAVAAWEDDAH
                     VDEQRARYRRRLELMAEVLAEAGIPGELPAGAFYLWVPAPDGDAWALTRRLAADGGAL
                     VAPGEFYGGGDHVRIAVVQPDDRLELVGRRLGRASR"
     gene            complement(3162..>4444)
                     /gene="hflX"
                     /locus_tag="VFP02_10950"
     CDS             complement(3162..>4444)
                     /gene="hflX"
                     /locus_tag="VFP02_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET9543594.1"
                     /translation="IDRSIREKIVLVGMTTPPDTAEDTEASMDELELLVDTAGADTAA
                     RVVQQRAAPDPKYYIGKGKAEELREVALETDCDTVVFDDELTPAQQLNLEKLLGRTAI
                     DRTAVILDIFAQNASSLEGKAQVQLAQYRYRLPRLRGSGARLSQQAGGIGTRRGPGET
                     QFEIDRRRIQRNITHLEHQLRDIRRQRDTKHKARHRSRIPQVALVGYTNAGKSTLLNH
                     LTDAGVLVEDRLFATLDPTTRRLELPGGSALLLTDTVGFVRKLPHQVVEAFKATLELV
                     AEADIVVHLVDATAADPLGQIDAVRIVLEEIGAGAIPEILAFNKADQGPAEALAAAHP
                     GSVAISARSGTGMDDLLRVIADRLHAGDQTVELLVPFDRGDVLAAAHRLGEVLVEEPG
                     EGGILVRARLDAEGRSRLAGYRLDGGDEPEPDLD"
BASE COUNT          606 a         1614 c         1605 g          619 t
ORIGIN      
        1 gcctcgacct cgccgtccgt gggctcaccc aggatggcga catctccctc caggaggtcc
       61 ggtcgctcga cgaacagctc ttccagcccg ctctccttcg ccgcgatctc ctcccgggcg
      121 tagaacacgt aggtcacgtc caccgcgggc tcgggcacgg ccagggccag ctcgaccatg
      181 acggccaggc cgcccttcat gtcggccgtg cccaggcccc agagggtcgt gccctcgcgg
      241 cggggggcgg cgttgccgtt ggccgggacc gtgtcggtgt ggccggcgag gaccagccgc
      301 agaggccggc ccaggttcgt ccgagccacc aggttgtccc ccacccgcgt ccgctccagg
      361 cccggcaccg cggccagcag gccgtcgagg tggtcggtga tggtggcctc gtcgcggctg
      421 agcgacggga tggcgcacag ctcctcggtg cgggcgagga gatcgggcat cagacggtcg
      481 acccgtggtc acggaggatg tcgttcaggg cgcccttgtc gtggcgctcg ccttcggtca
      541 ggcgcttgat gaccagcaca cagggcaggc cgaacgtgcc gccggcgaac gctctcggcc
      601 gggtggcggt gacggccacg gaccaggggg ggacgtggcc ccggctgatc tccgcgccgc
      661 tctcggcgtc gatcaccggc accgacgcgg tgaggatcga gcccgccccg agcaccgtcc
      721 cagtcccgac cgtcgcgccc tcggcgacga tgcagcggga gccgatgtaa cactcgtcgc
      781 cgatcatcac cggcgcggcc tgcaccggct ccagcacccc cccgatgccg acgccgccgg
      841 acaggtggac gttgcggccg atctgggcgc acgacccgac ggtggcccag gtgtcgacca
      901 tcgaccccga cgccacgtgg gcaccgatgt tcacgtagct cggcatcatg accacgccgc
      961 ggtccaggaa cgacccccaa cgggccgacg cgccgggcac cacccgcacg cccttggcct
     1021 cgtagtccga cttcagcggc agcttgtcgg cgtattcgaa cgggccgagc tcgatggtct
     1081 ccatgccctg gacccggaag agcaggagca cggcccgctt cagccactcg ttgacggtga
     1141 ccgcgccctc accgtcccat tcggccacgc gtgccgcccc cgagtcgagg aggtcgactg
     1201 cctcgtgcac gatgcgccgg gcatcgtcgt ccccgggtcc caagtcgtcc cgtcgagccc
     1261 agagatcgtc gatgctcgcc tgcagatcgc tcatgacggc cgcaggctac cggcggccgg
     1321 cgacgcgcct aggcggtcgg cggtgggggg tagcccgccg ggggtggcgg attgcccgac
     1381 caggcgccgg ggctcgccgg aggtggcggg ttccaggggc ccggctgcgg tgcaccgccg
     1441 gggccgttcc gggcatccag ccaggccctc tggcggaact tgcgccgtcg gacaccggtg
     1501 acgatgacca ggacgatgcc cagcacgatg ccgatgccgg cgatgacgaa gccgccgacg
     1561 atggacgcca ccaggtcact cgcgaccgag ggcccgaacg ccgccgtcgt ggccgtggtc
     1621 gctggcccgc cgcccacccg gacgtcgtac tcccccgtct cctcgatctc gaaggtcaac
     1681 tgggctcggc cggtgtggtt gccgaagtcg taggtgaact ccgagacgta gggctcgaac
     1741 tcgatctcgt cggcgccttc ctggcccgcc ggccgcatgc ggacgtcgtc gacgaagagc
     1801 gagacggcgt cgcccccacc ccgctcggcg tagacgacgt agtccccgtc gtcgagctgg
     1861 atggtgctga tctcgcccac cgggacccgc tcgaagtcgt cgatggcatc caggaaggac
     1921 gagaagctca cccatcccca gacgatgccg ccgatgatcc cgccggcgat caggaggccc
     1981 ccgagccagt agcccagcga ggacggtcct tggggctggt ccatcggata cgccccatcc
     2041 gcaccgaacg gactggtcat ctcgaggccc ttcccagtcg tcgacccacc agctccaggc
     2101 ggtcgtcggg ttgcaccacg gcgatgcgga cgtggtctcc tccaccgtag aactcgccgg
     2161 gagccaccag cgcgcctccg tccgcggcca ggcgccgggt gagggcccaa gcatcgccat
     2221 cgggcgcggg cacccacagg tagaacgcgc ccgccggcag ctcaccgggg atgcccgcct
     2281 ccgccagcac ctcggccatg agctccagcc gccgccggta gcgggcccgc tgctcgtcga
     2341 cgtgggcgtc gtcctcccag gcggccaccg cggcggcctg tacaggaccc gggaccatca
     2401 tccccgcgtg cttgcgcacc tcccgcaagt aggtcaccag ctcggggtcg ccggcgtaga
     2461 agccggcccg cacgccggcc agattcgagc gcttcgagag ggagtgcacg gccaacacac
     2521 cctcgatgcc gtgctcgagg atgctgcgcg gaggcccgtc ccaggtgaac tccacgtagc
     2581 actcgtccga caggaccggc accccgtgag cccggcccca cgccgcggcg gacccgaggt
     2641 cgtccaaccg cccggccggg ttgcccggcg agttgaccca caggcagagc gcccgctcgg
     2701 cgtcggcggc gctgacggcg tcgaggtcca gcgtgccgtc cggggagggc ggcacggcga
     2761 cggaccggca tcgggccagg gtggccccca tggcgtagct ggggtaggcg acctgcgggt
     2821 gcaggaccgt gtcgcggtcg ggggcccgca gcttgaggaa cgacggcgtc ccgaccacga
     2881 actccttggt ccccacgcag gcggccaggc tgtcgggatc gaccgtcacc cccaggcggc
     2941 gggccaacca gcccgctgcg gcgcgccggt acggggcgct gccgatggac ggggggtagc
     3001 cccgctcggc gccggagccg gccagggcct cgaccaccgc agccggcggt gggtcgttcg
     3061 gggtcccgat ggacaggtcg acgcagccac cgggcagggc gtcggccacc gccttcagct
     3121 cgtcgagccg gtcgtagggg tagggcggcg gctggaagcc ctcagtcaag gtcgggctcg
     3181 ggctcgtcgc ccccgtcgag gcggtagccg gccagccggg accgaccctc ggcgtcgagg
     3241 cgggctcgga cgagtatccc gccctcccct ggctcttcga ccaggacctc acccagacga
     3301 tgggcggcgg cgaggacgtc gccgcggtcg aagggcacga gcagctcgac ggtctggtcc
     3361 ccggcgtgga ggcggtcggc gatgacccgc agcaggtcgt ccatgccggt gcccgaccgg
     3421 gccgagatgg ccaccgagcc ggggtgggcg gcggccagcg cctcggccgg gccctggtcg
     3481 gccttgttga acgccaggat ctccgggatc gcccccgccc cgatctcctc caagacgata
     3541 cgaacggcgt cgatctggcc cagtgggtcg gccgcggtgg cgtcgacgag gtggaccacg
     3601 atgtcggcct cggccaccag ctcgagggtg gccttgaagg cctccaccac ctggtggggc
     3661 agcttgcgca cgaagccgac cgtgtcggtc aggagcaacg cgctcccacc gggcagctcc
     3721 agccgccggg tcgtggggtc cagggtggcg aacagccggt cctcgaccag gaccccggca
     3781 tcggtcaggt ggttgagcag ggtcgacttg ccggcgttcg tgtagccgac cagcgccacc
     3841 tgggggatgc gggagcggtg ccgggccttg tgcttggtgt cccgctgacg gcggatgtcg
     3901 cggagctggt gctccaggtg ggtgatgttg cgctggatgc gtcggcggtc gatctcgaac
     3961 tgcgtctcgc ccgggcctcg gcgggtgccg atgccgccgg cctgctggga gagccgggcc
     4021 ccgctccccc ggaggcgggg cagccggtac cggtactggg ccagctggac ctgggccttg
     4081 ccctcgaggc tggaggcgtt ctgggcgaag atgtcgagga tcaccgcggt gcggtcgatt
     4141 gcggtccgcc cgagcagctt ctcgaggttg agctgctggg ccggggtcag ctcgtcgtcg
     4201 aagaccaccg tgtcgcagtc cgtctccagg gccacctcgc gcagctcctc ggccttgccc
     4261 ttgccgatgt agtacttggg atcgggcgcg gcccgttgct ggacgacccg ggccgcggtg
     4321 tcggctccgg cggtgtcgac gaggagctcc agctcgtcca tcgacgcctc ggtgtcctcg
     4381 gccgtgtcgg gtggggtggt catgcccacc aggacgatct tctcccggat ggaccggtcg
     4441 atca
//
LOCUS       DASTFV010000600         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2284408, whole genome shotgun sequence.
ACCESSION   DASTFV010000600 DASTFV010000000
VERSION     DASTFV010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2284408"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VFP02_10955"
     CDS             <1..876
                     /locus_tag="VFP02_10955"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9543595.1"
                     /translation="RTSLWTAVGMPKGPTTWDELLQGGAEIKKSKGTQLGIGMSQEID
                     SNMAGRALMWSYGASVQDENEQVVINSEETVAAVEFMTKLFKQAMTDEVFSWNAASNN
                     QGLVAGKLSYILNSISAWRTAQGANPDVANDVGFVPALRGPADARAAQHVLYNWIIPK
                     HAANPDAAKEFLLYYTENFASATWHSKLYDFPAWAERVPQLDGWLAKDPFGANPADKL
                     AFLKDATGWSTNIGYPGPSNTAIGEVFGTFVIPNMFASAARGDASPKEAVAQAEAQIK
                     PIFEKWRKRGLVGGT"
     gene            890..1933
                     /locus_tag="VFP02_10960"
     CDS             890..1933
                     /locus_tag="VFP02_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9543596.1"
                     /translation="MTKVVEVRGLVKQFEGGRVNAVDGIDLATEEGEYLVLLGPSGCG
                     KTTLLRTIAGLEQPSEGEVRIDGQVVNDLPPRARKIAMVFQSYALYPHKSVLQNIVFP
                     LKAERMDKDEQVRKARWAAELLGIDQLLDRRPRQLSGGERQRVALARAMVREPQVFLL
                     DEPLSNLDAKLRTSARDELKRFQQRVGTTTIYVTHDQAEAMGLGDRIAVMDHGRVRQL
                     GKPVEVYDDPADTFVATFIGSPPMNLVPRDGRFVGFRPEHLLPAEHVGGPERRLVPFK
                     VDRIEYLSGDRHVYGTATRIGEETRVIARLPATVATPIAAGETYEFAVHPDRLRFFDA
                     ESGHRADPVPVGG"
     gene            1938..>2393
                     /locus_tag="VFP02_10965"
     CDS             1938..>2393
                     /locus_tag="VFP02_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9543597.1"
                     /translation="MDAATTERPGVRAGKRPARHFADNERQMAIWFLLPSILYVIALV
                     AVPFFLAIGFAFSDVSAGDPSFQWAGLRTFERALDDPVFWRSLRNTFVFTVVSMALIV
                     VVGKVLANILVADFRGKWLIRFLVLLPWTTPVALSAVAWLWMLDSVFSPI"
BASE COUNT          372 a          874 c          803 g          344 t
ORIGIN      
        1 cggacctcgc tctggacggc tgtgggcatg cccaagggcc ccaccacatg ggacgagctc
       61 ctgcagggcg gcgccgagat caagaagagc aaggggaccc agctcggcat cggcatgtcc
      121 caggagatcg actccaacat ggccggtcgg gccctgatgt ggtcctacgg cgcctcggtc
      181 caggacgaga acgagcaggt ggtgatcaac tccgaggaga ccgtggccgc cgtcgagttc
      241 atgaccaagc tgttcaagca ggccatgacc gacgaggtct tctcctggaa cgccgcctcc
      301 aacaaccagg gcctggtcgc cggcaagctc tcctacatcc tcaactcgat ctcggcctgg
      361 cggaccgccc agggggccaa ccccgacgtg gccaacgacg tcggcttcgt cccggcgctg
      421 cgcgggcccg ccgatgcccg cgccgcccag cacgtgctct acaactggat catccccaag
      481 cacgcggcca accccgacgc ggccaaggag ttcctgctct actacaccga gaacttcgcc
      541 tcggccacct ggcactccaa gctgtacgac ttcccggcct gggccgagcg ggtgccccag
      601 ctcgacggct ggctggccaa ggaccccttc ggggccaacc cggccgacaa gctggccttc
      661 ctcaaggacg cgaccggctg gagcaccaac atcggctacc cggggccgtc caacaccgcc
      721 atcggcgagg tgttcgggac cttcgtgatc ccgaacatgt tcgccagcgc ggcccgcggc
      781 gacgcctcgc ccaaggaggc cgtggcccag gccgaggccc agatcaagcc gatcttcgag
      841 aagtggcgga agcgcggcct ggtcggcggc acgtgatcgg cggccggccg tgacaaaggt
      901 cgtcgaggtc cgggggctcg tcaagcagtt cgagggcggg cgggtcaacg ccgtggacgg
      961 catcgacctg gccaccgagg agggcgagta cctcgtcctc ctcggcccct cggggtgcgg
     1021 caagaccacc ctgctgcgca ccatcgccgg gctggagcag ccgagcgagg gggaggtccg
     1081 catcgacggg caggtcgtca acgacctgcc gccgcgggcc cgcaagatcg ccatggtctt
     1141 ccagagctac gccctgtacc cgcacaagag cgtcctccag aacatcgtct tcccgctcaa
     1201 ggccgagcgg atggacaagg acgagcaggt ccgcaaggcc cgctgggcgg ccgagctgct
     1261 cggcatcgac cagctgctgg accgccggcc ccgccagctg tcgggcggcg agcgccagcg
     1321 ggtcgccctg gcccgggcga tggtccgcga gccgcaggtg ttcctgctgg acgagccgct
     1381 ctccaacctg gacgccaagc tgcgcaccag cgcccgcgac gagctcaagc gcttccagca
     1441 gcgggtcggg acgaccacca tctacgtgac ccacgaccag gccgaggcca tgggcctggg
     1501 cgaccggatc gcggtcatgg accacggccg ggtgcgccag ctcggcaagc cggtcgaggt
     1561 ctacgacgac ccggccgaca ccttcgtggc caccttcatc ggctccccgc cgatgaacct
     1621 ggtgccccgc gacggccgct tcgtcgggtt ccgccccgag cacctgctgc cggccgagca
     1681 cgtcggaggg cccgagcgaa ggttggtgcc gttcaaggtg gaccggatcg agtacctctc
     1741 cggcgaccgg cacgtgtacg gcacggccac gcgcatcggc gaggagaccc gggtcatcgc
     1801 ccggctgccg gccacggtcg cgaccccgat cgcggccggc gagacgtacg agttcgccgt
     1861 gcaccccgac cggctgcggt tcttcgacgc cgaaagcggg caccgggccg accccgtccc
     1921 ggtcgggggc tgaggccatg gacgcggcca ccaccgagcg ccccggcgtc cgcgccggga
     1981 aaaggccggc ccggcacttc gccgacaacg agcgccagat ggcgatctgg ttcctgctgc
     2041 cgtcgatcct gtacgtgatc gcgctcgtgg cggtgccgtt cttcctggcc atcgggttcg
     2101 ccttcagcga cgtgtccgcc ggcgacccca gcttccaatg ggccggcctg cgcaccttcg
     2161 agcgcgccct tgacgacccg gtgttctggc gctcgctgcg caacaccttc gtcttcacgg
     2221 tggtctcgat ggccctgatc gtggtggtcg gcaaggtcct ggccaacatc ctggtggccg
     2281 atttccgcgg caagtggctg atccggttcc tggtcctgct gccctggacg accccggtgg
     2341 ccctttcggc ggtggcctgg ctgtggatgc tggactcggt cttcagcccg atc
//
LOCUS       DASTFV010000601         3524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2287077, whole genome shotgun sequence.
ACCESSION   DASTFV010000601 DASTFV010000000
VERSION     DASTFV010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2287077"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFP02_10970"
     CDS             <1..342
                     /locus_tag="VFP02_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543598.1"
                     /translation="VDPDTRTFDADPANEAALGDFLHGRLHGVGGVRASKTCLYTLTP
                     DRDFVLDRMPEAPEVLVALGAAHSFKFAAWFGLTLAQLALDGAAAADLSPFRIDRPAL
                     TDQDHQPTWLV"
     gene            376..1437
                     /locus_tag="VFP02_10975"
     CDS             376..1437
                     /locus_tag="VFP02_10975"
                     /inference="COORDINATES: protein motif:HMM:NF014954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9543599.1"
                     /translation="MLILDADGLTAALQAAGTLAPDVRVRSVGREPVGTGQMGDCLRL
                     ELRYDGPTDAPTTIVAKLPSSDETSRATGVAMRTYEVEVRFYQELAPRLPVRAPRCHH
                     AEIDIGSGDFVLLLEDLAPAVQGDQVAGCTVDQARLVVAEAARLHAPLWGDASLDDLE
                     WAVRWSPDAQDAMQAMMTVLWPNFVERYGPDLDHDVVEMGGRFVRRLRSFYAHRPEPH
                     TVIHNDFRLDNLLFGTVAGGPPVAVVDWQTVGVGPAALDVSYFLGAGLSVEDRRAHEE
                     ELVGEYHAALVAGGVTDYDGDRCWTDYRAFAFAGFHMAVLASMLVERTDRGDAMFLAM
                     AGRHGRQVLDLDAEEFLHD"
     gene            complement(1450..3342)
                     /locus_tag="VFP02_10980"
     CDS             complement(1450..3342)
                     /locus_tag="VFP02_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HET9543600.1"
                     /translation="MPVAGIPAPHLRRGLLGLAAVLVAVVAIAVATTETAGGEAPLEC
                     DGGLYVTTGSPDDMTLTRVDQETGALAAVGDGGLVANALGHNPRDDFLYGIDRNAPHH
                     VVRVSAGGDEVDLGPAKGAPASWVLTFVGTFLANGHYLVLGDNAPARASRGTVPGTWA
                     EIDVTAGAPEVIRTFSHPSIGNNDLQDIALNPVDGELYGHSITRHRIVRINPTTGAAT
                     GIGPAFARPGNAGSAFFDSFGRMWLYGSGRSVGAQDTLYRIDHVGRDKPAVVAQGPAV
                     TNSDGASCPFTIGMEKTVDRPAACAGTTVTYRYEITNEAVDADPEEGAATVSADFVDQ
                     LPDDGRTFVAGSLVNPLGGDVAAYGGTDRLRIDDLAIPHDSTATIQVAVALPADMEAG
                     TVMNQARLVDLTGNLGTHVLSEFPGTPRLPDATPLAVLNCADISVAKTVDVEVAGPGD
                     ELTYTVRVTNHGPSDAPAVDSVADSLPAGLTFVAASHGGTLTSGGTVRWSAFALAVGA
                     HRDLTVAASADENIRATTKHDGDLDNIAAVQHPGDPNPGNDHDTAVVPIDHPDLVVDK
                     DDDRDTVDEPDDRPAPTRADEPHPGHDGPVPALPRTGAAALSWSAIGAGLIALGLAAR
                     SFRSRA"
BASE COUNT          468 a         1191 c         1331 g          534 t
ORIGIN      
        1 gtcgaccccg acacccgcac cttcgacgcc gatccggcca acgaggcggc gctgggcgac
       61 ttcctccacg gccggctcca cggcgtcggc ggcgtccgcg cctcgaagac ctgcctctac
      121 accctcacgc cggatcggga cttcgtgctc gaccggatgc cggaggcccc cgaggtgctg
      181 gtcgccctcg gcgccgccca cagcttcaag ttcgcggcct ggttcggcct gaccctggcc
      241 cagttggcgc tggacggggc ggccgccgcc gacctgtcgc ccttccggat cgaccgcccg
      301 gccctgaccg accaggacca ccagccgacc tggctggtct aggccggcct tccatcgcgg
      361 ccatgatggt cggcgatgtt gatcctcgat gccgacgggc tgacggccgc gctccaggcc
      421 gccgggacgc tggcgccgga cgtccgggtg aggagcgtcg gccgcgagcc cgtgggcacc
      481 ggtcagatgg gtgactgcct gcggttggag ctccgctacg acggcccgac cgatgccccc
      541 accacgatcg tggccaagct tccgtcctcg gacgagacca gccgggccac cggtgtggcc
      601 atgcggacct acgaggtcga ggtgcgcttc taccaggagc tggccccccg cctgccggtg
      661 cgggctcccc gctgccacca cgcggagatc gacatcggct ccggggactt cgtgctcctg
      721 ctcgaggacc tcgcccctgc ggtccagggc gatcaggtcg ccggctgcac ggtcgaccag
      781 gcccgcctgg tcgtggccga agccgcccgc ctccacgcgc cgctctgggg cgatgcgagc
      841 ttggacgacc tcgagtgggc cgtgcggtgg tcgcccgacg cccaggacgc catgcaggcg
      901 atgatgaccg tgctctggcc caacttcgtc gagcgctacg gtcccgacct cgaccacgac
      961 gtggtcgaga tggggggccg gttcgtccgg cgcctgcggt cgttctacgc gcaccgaccc
     1021 gagccccaca cggtcatcca caacgacttc cgcttggaca acctgctctt cggcaccgtg
     1081 gccgggggcc cgccggtcgc cgtcgtggac tggcagaccg tgggtgtcgg gccggctgcg
     1141 ctcgacgtca gctacttcct gggggccggt ctctccgtcg aggatcgccg ggcccatgag
     1201 gaggagctgg tcggtgagta ccacgccgct ctcgtcgccg gcggggtcac cgactatgac
     1261 ggggatcgct gctggaccga ctaccgggcc ttcgccttcg ccggcttcca catggcggtg
     1321 ctggcctcga tgctcgtgga gcggaccgac cgcggcgacg ccatgttcct ggccatggcc
     1381 ggccgccacg gccgtcaggt cctcgacctg gacgcggagg agttcctcca cgattgacgg
     1441 agtccgtcgt cacgctctcg agcggaacga acgggcggcg aggccgaggg cgatcagccc
     1501 ggcaccgatg gcggaccagg agagggcggc ggcgccggtg cggggcagcg ccgggacggg
     1561 cccgtcatga ccagggtggg gctcgtcggc gcgggttgga gcgggacggt cgtccggctc
     1621 gtccacggtg tcccggtcgt cgtccttgtc gacgaccagg tccggatgat cgatcggcac
     1681 cacggccgtg tcgtggtcgt tgcccgggtt cgggtcgccg ggatgctgga cggcggcgat
     1741 gttgtcgagg tcgccgtcgt gtttggtcgt ggcgcggatg ttctcgtcgg cggaggcggc
     1801 gaccgtgaga tcccggtggg cgccgacggc cagagcgaag gcggaccagc gcaccgtgcc
     1861 gcccgatgtg agcgtgcccc cgtgggaggc ggccacgaag gtgaggccgg ccggcaggct
     1921 gtcggcgacc gagtcgacgg ccggggcgtc actgggcccg tggttggtca cgcggacggt
     1981 gtaggtgagc tcgtcaccgg gtccggccac ctcgacgtcg accgtcttgg ccacgctgat
     2041 gtcggcgcag ttcaggacgg cgagaggcgt ggcgtccggc agccgtggcg tcccggggaa
     2101 ctcggagagg acatgagtac cgaggttgcc ggtcaggtcg acgagccgag cctggttcat
     2161 caccgttccc gcttccatgt ccgcggggag ggcgacggcg acctggatgg tggccgtgct
     2221 gtcgtgggga atggccaggt cgtcgatccg gagtcggtcg gtgccgccgt aggccgccac
     2281 gtcgccgccg agggggttga ccagtgatcc ggccacgaag gtgcgaccgt cgtccgggag
     2341 ctggtccacg aagtcggccg agacggtcgc ggcaccctcc tcggggtcgg cgtcgacggc
     2401 ctcgttggtg atctcatagc ggtaggtgac cgtggtgccc gcgcaggcgg ctggccggtc
     2461 gaccgtcttc tccatgccga tggtgaacgg gcaggaggcg ccgtcggaat tggtgacggc
     2521 gggaccctgg gcgacgaccg cgggcttgtc ccggccgacg tggtcgatgc ggtagagcgt
     2581 gtcctgggcg ccgacggacc ggcccgaccc gtagagccac atccgcccga agctgtcgaa
     2641 gaacgcgctg ccggcattgc cgggcctggc gaaggccgga ccgatcccgg tggccgcccc
     2701 ggtggtcggg ttgatgcgca cgatgcggtg gcgggtgatg ctgtggccgt agagctcgcc
     2761 gtcgacgggg ttgagggcga tgtcctggag gtcgttgttg ccgattgaag ggtgcgagaa
     2821 cgtccggatc acctcagggg cgccggccgt gacgtcgatc tcggcccagg tgccgggcac
     2881 ggtcccccgg gaggcccggg cgggcgcgtt gtcgccgagc acgaggtagt ggccgttcgc
     2941 caggaaggtc ccgacgaagg tcagcaccca cgaggccggc gcgcccttgg ccgggccgag
     3001 gtcgacctcg tcgccgccgg ccgagacccg tacgacgtgg tgtggggcgt tgcggtcgat
     3061 gccgtagagg aagtcgtccc gcgggttgtg accgagggca ttcgccacca gtccgccgtc
     3121 gccgaccgcc gcgagcgcgc cggtctcctg gtcgaccctc gtgagggtca tgtcgtccgg
     3181 tgagccggtc gtgacgtaga gcccgccgtc acactccagc ggggcctcgc cgccggccgt
     3241 ctccgtggtg gcgacggcga tcgccaccac cgcgaccagc acggcggcca ggcccagcag
     3301 gccccggcgg aggtgagggg cagggatccc ggcgacgggc atgggcgacc tcgtgaggag
     3361 agacatgaaa ctgcatgaga tcatctccat gtgagaaatg ggagtcaagg aaaaataatg
     3421 aaagatcagc aaaagcccgg gtgagtgctc cccgcgggcg gtgccgtcgc ctacggtcca
     3481 ggcgtgccgg aacgcctgct cgagggtcgg gtcgccgtgg tcac
//
LOCUS       DASTFV010000602         6083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2298795, whole genome shotgun sequence.
ACCESSION   DASTFV010000602 DASTFV010000000
VERSION     DASTFV010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6083
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2298795"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFP02_10985"
     CDS             complement(<1..546)
                     /locus_tag="VFP02_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET9543601.1"
                     /translation="MAVGTTGTYEVATPVYDGPFELLLHLILREEVDLYEVSLLDIVD
                     AYLAEFERMEHCDLDTATEFLLIASTLVELKSRRLLPVDDDVDLDDELALWEERDLLL
                     SRLLECKTFKDAAVVLRRLHRDGARTYPRLAGIEEQFVSLAPDLLAGVDAGDVRRAYV
                     EVLTRPQPRVDLFHIAAIKVNV"
     gene            603..1586
                     /gene="trpS"
                     /locus_tag="VFP02_10990"
     CDS             603..1586
                     /gene="trpS"
                     /locus_tag="VFP02_10990"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET9543602.1"
                     /translation="MPRVFSGIKPTGEVHLGNLLGALRNWVSDQDTHDCVYCVVDLHA
                     LTVPQDPAELTATSLSLAQKLISVGLDPDRCTLFVQSHVHEHAELAWIMECTVAYGEL
                     SRMTQFKDKSNRAEFVSGGLFTYPALMAADILVHDTDRVPVGDDQRQHLELTRDVAGR
                     FNSRYGETFVIPEAYIPKAGARIMDLQRPTDKMSKSEDSPQGTIDLLDDPAVIEKKLK
                     RAVTDSGTDVTFDPQNRPGVANLLSILAAATDRDPKAVAEDYTQFGPLKADTAEAVIE
                     LLRPVQARYRELEADPGATHALLAQGADKARAIAAAVVARTRKAIGLLPPA"
     gene            complement(1590..2102)
                     /locus_tag="VFP02_10995"
     CDS             complement(1590..2102)
                     /locus_tag="VFP02_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543603.1"
                     /translation="MLHLVRRFFASLLPIGASAVDDAWAREQLTEAEQELWTRLARVD
                     RRHSAAVARKVDAELGVRATTPVLAAALLHDVGKTISHLGTFGRVVATLSEAAAGEDT
                     ARHWIETRGFTRRVGQYVLYPELGVDLLRMAGSDELVLAWSREHHLPEEDWTVPVDVG
                     RVLQRADDAS"
     gene            complement(2122..2430)
                     /locus_tag="VFP02_11000"
     CDS             complement(2122..2430)
                     /locus_tag="VFP02_11000"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HET9543604.1"
                     /translation="MADPVESTVRASLEEEREQLLGQLSEPDEAFDENFADSGQVAAE
                     QGEAQALAASLRDQLDAVEQALTKLDEGTYGLCERCQKPIPEARLEAVPASRFCIEHA
                     "
     gene            complement(2440..3378)
                     /locus_tag="VFP02_11005"
     CDS             complement(2440..3378)
                     /locus_tag="VFP02_11005"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HET9543605.1"
                     /translation="MTASTLVLPLEMEEYLTWLATEKGRSVNTLTAYRRDLKGYAGWL
                     HERGTGLAEVTPGDVEAYVAVLREAGKAPATMARAIVAVRSLHAFAAAEGLLPADPAA
                     DVEPPSVPRGLPKGLSEAEVESLLDVAGTDPVARRDRAILEVLYGTGMRIAELVGLCL
                     GDVDLGDALVRVLGKGSKERVIPLGRLARASLVDWLDTGGRPAMAPARWARRGDEEAV
                     FLNQRGGRLTRQGAWGIVSEHGRRAGLERTLSPHALRHSCATHMLDHGADIRAVQELL
                     GHASISTTQIYTGVSVERLFAVYAAAHPRAKASTAG"
     gene            complement(3375..3911)
                     /locus_tag="VFP02_11010"
     CDS             complement(3375..3911)
                     /locus_tag="VFP02_11010"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9543606.1"
                     /translation="MSPFTKRGEREVWSGAVISVVQGTFVSPSGATFERDVVHHPGAV
                     AVVPVDGDEVVLVRQYRAAVDLEILEIPAGKRDVAGEAPELTAHRELAEEVGLTAGRL
                     VKLAEFYNSIGFSDELSHVFLGTELSPVPDDRQGEEEEHMTVERIRLDDVPALVAAGE
                     LVDAKTVIGLLMAREHLA"
     gene            complement(3916..5565)
                     /locus_tag="VFP02_11015"
     CDS             complement(3916..5565)
                     /locus_tag="VFP02_11015"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET9543607.1"
                     /translation="MAKHVFVTGGVASSLGKGITAASLGRLLKSRGLRVTIQKLDPYI
                     NVDPGTLNPFEHGEVYVTDDGGETDLDLGHYERFIDESLTRDSNATTGSIYQSVLAAE
                     RRGDYLGKTVQVIPHITNEIKRRIKVLATDDVDVVITEVGGTVGDIEILPFLEAIRQF
                     RLDVGRDNVCYVHLTLVPLIGPAGEQKTKPTQHSVTELRSRGIQPDVIVCRSADEIST
                     DLKRKISNLCDVEVRAVVNAADADSLYDVPLVLHEEGMDAVVCEILRLDDRPVDLTEW
                     ETLVDRVHAADRTVRVGVVGKYTSLPDAYLSVIESLNHAGFAHGAKVEIDLISAEEVE
                     GLLGAGRLKDLDGIVIPGGFGERGIEGKIAAAGYAREEGIACLGLCLGLHTMVIEYAR
                     DVLGLEGANSSEFDAATPHAVIDLMDAQRDVTDKGGTMRLGAYVAKLVPDSQVARAYG
                     KDVVSERHRHRYEFNPRYRTRFEEGGLLCSGESPDGRLVEFIELPGHPFWIATQAHPE
                     LKSRPNRPAPLFREFVGAALERSEGRRPHLFEPEAVSQPAG"
     gene            5636..>6083
                     /locus_tag="VFP02_11020"
     CDS             5636..>6083
                     /locus_tag="VFP02_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543608.1"
                     /translation="MTDASPAPADAPDRAALAEPATTRRDLLWLGALTLALRLPTFFA
                     TKHLTFDDGVFGASAVAMRNGGVPFRDVFSSQGPLFLPLVWIADTLGLHTLDGPRLLG
                     LASGLALVIVIYFAGRELGGTTGARIAGGLAAVTGSSIAVTGSIAAD"
BASE COUNT          895 a         2212 c         2090 g          886 t
ORIGIN      
        1 cacgttgacc ttgatcgccg cgatgtggaa gaggtcgacc cggggctggg ggcgggtcag
       61 cacctcgaca taggcccggc gcacgtcgcc ggcgtcgacc ccggccagca gatcaggggc
      121 cagggagacg aactgctctt cgatgccggc caggcggggg taggtgcggg cgccgtcgcg
      181 gtgcaggcga cgcaggacga cggcggcgtc cttgaacgtc ttgcactcca gcaggcggga
      241 caggagcagg tcccgctcct cccaaagggc caactcgtcg tcgagatcca cgtcgtcatc
      301 caccggcagg agccggcggg acttcaactc caccagcgtg gaggcgatga gcaggaactc
      361 ggtggccgtg tcgaggtcgc agtgctccat ccgctcgaac tcggccaggt aggcgtcgac
      421 gatgtcgagc aggctgacct cgtacaggtc cacctcctcg cgcaggatca ggtggagcag
      481 cagttcgaag ggcccgtcgt agacgggcgt cgccacttcg taggtccccg tcgtgcccac
      541 agccatgcgg ccgaggatac gggtggggtg acggaggggc gcggacccta gattcagcgc
      601 tcgtgccccg cgtcttctcc ggaatcaagc ccacgggcga ggtccacctc ggcaacctgc
      661 tgggggccct gcgcaactgg gtgagcgacc aggacaccca cgactgcgtc tactgcgtgg
      721 tcgacctcca cgccctcacg gtcccccagg acccggccga gctcaccgcc accagcctct
      781 cgctggccca gaagctgatc tcggtcggcc tcgacccgga ccgctgcacg ctgttcgtcc
      841 agagccacgt acacgagcac gccgagctgg cctggatcat ggagtgcacg gtcgcctacg
      901 gcgagctcag ccgcatgacc cagttcaagg acaagtcgaa ccgggccgag ttcgtgtccg
      961 gcgggctctt cacctatccg gccctcatgg cggccgacat cctcgtccac gacaccgacc
     1021 gcgtaccggt gggcgacgac caacgccagc atctcgagct cacccgggac gtcgccggcc
     1081 gcttcaactc ccgctacggc gagacgttcg tgatccctga ggcctacatc ccgaaggcgg
     1141 gcgcccggat catggacctg caacgcccca ccgacaagat gtccaagtcc gaggactccc
     1201 ctcaagggac gatcgacctg ctcgacgacc cggcggtgat cgagaagaag ctcaagcggg
     1261 ccgtcaccga ctccggcacc gacgtcacct tcgacccgca gaaccgcccc ggcgtggcca
     1321 acctgctctc gatcctggcc gccgccaccg atcgggaccc caaggccgtg gccgaggact
     1381 acacgcagtt cggccccctc aaggccgaca ccgcggaggc cgtgatcgag ctcctccgcc
     1441 ccgttcaggc ccgctaccgc gagctggagg ccgatcccgg cgccacccac gccctcctgg
     1501 cccagggcgc cgacaaggcg agggcgatcg cggccgctgt cgtggcccgg acgcggaagg
     1561 ccatcggcct cctacctccc gcctgaccct cagctggcgt cgtcggctcg ctgcaggacc
     1621 cggccgacgt cgacgggcac ggtccagtcc tcctcgggca ggtggtgttc gcgggaccag
     1681 gcgagcacga gctcgtcgct gccggccatt cgcagcaggt cgaccccgag ctcgggatag
     1741 agcacgtact ggccgacgcg acgggtgaag ccccgggtct cgatccagtg gcgagcggtg
     1801 tcctcaccgg cagcggcttc ggacagcgtc gccacgacgc ggccgaaggt ccccagatgc
     1861 gagatcgtct tgcccacgtc gtggagtagg gccgcggcca ggaccggggt cgtagctcgc
     1921 accccgagct cggcgtcgac cttgcgggcc acggccgccg aatggcgccg gtcgacccgg
     1981 gccaggcgcg tccacagctc ctgttcggcc tcggtcagct gctcccgggc ccaagcgtcg
     2041 tcgacggcgc tcgcgccgat cggcagcagc gaggcgaaga agcgccgcac caggtgcagc
     2101 actcgtttca cagcacctcg atcaggcgtg ctcgatgcag aagcggctgg cgggaacggc
     2161 ttcgagccgg gcctcgggta tgggcttctg gcagcgctca cagagcccgt acgtgccctc
     2221 gtcgagcttg gtcagggcct gttccacggc gtccagctgg tcccgcaggg aggcggccaa
     2281 ggcctgagcc tcgccctgct cagcggccac ctgaccggag tcggcgaagt tctcgtcgaa
     2341 cgcctcgtcc ggttcgctga gctgtccgag cagctgctcg cgctcctcct cgagtgaggc
     2401 gcgaaccgtc gactccactg gatcggccat caacggaccc taccccgcgg tcgaggcctt
     2461 ggcgcgggga tgggcggcgg cgtacacggc gaagaggcgc tcgaccgaca cccccgtgta
     2521 gatctgcgtg gtgctgatgg aggcgtggcc caacagctcc tggacggccc ggatgtcggc
     2581 gccgtggtcg agcatgtggg tggcacagct gtggcgcagg gcatgggggc tgagcgtgcg
     2641 ttcgaggccg gcccggcggc cgtgctcgga cacgatgccc caggccccct gccgggtcag
     2701 gcgtccgcct cgctggttga ggaagaccgc ctcctcgtcg ccccggcggg cccaccgggc
     2761 gggcgccatc gccggccggc ctccggtgtc cagccagtcg accagcgagg cccgggccag
     2821 acggccgagg gggatgaccc gttccttgct gcccttgccg agcacccgga cgagggcgtc
     2881 gccgaggtcg acatcgccga ggcagagacc gaccaactcg gcgatgcgca tgccggtgcc
     2941 gtagagcacc tcgaggatgg cccggtcccg tcgggccacg gggtcggtgc cggccacgtc
     3001 caggagcgac tcgacctccg cctccgacag ccccttgggg agcccacggg ggacactcgg
     3061 cggctcgacg tcagccgccg ggtccgccgg aagcaggcct tcagccgcgg cgaacgcgtg
     3121 cagcgagcgc acggcgacga tcgcccgggc catggtggcc ggggccttgc cggcctcgcg
     3181 cagcacggcg acgtaggcct cgacgtcccc gggcgtcacc tccgccaggc ccgtgccccg
     3241 ctcgtgcagc cagcccgcgt aacccttcag gtcccgccgg taggcggtca gcgtgttcac
     3301 cgaccgtccc ttctcggtgg ccaaccaggt caggtactcc tccatctcca gaggcaggac
     3361 cagggtcgat gccgtcacgc caggtgttcc ctcgccatca gcagcccgat gacggtcttg
     3421 gcgtcgacca gctcgccggc ggcgaccagg gcgggaacat catcgaggcg gatgcgctcg
     3481 acggtcatgt gctcctcttc ctcgccctgc cggtcatcgg ggaccgggga cagctcggtg
     3541 ccgaggaaca catgggacag ctcgtcgctg aacccgatcg agttgtagaa ctcggccagc
     3601 ttcaccaggc gaccggccgt cagccccacc tcttcggcca gctcgcgatg ggcggtgagc
     3661 tcgggggcct cgccggcgac gtcccgcttg ccggcgggga tctcgaggat ctccaggtcc
     3721 accgcggccc ggtactggcg cacgagcacg acctcgtcgc cgtccacggg caccaccgcg
     3781 accgcgcccg gatggtggac cacgtcccgc tcgaaggtgg cccccgacgg cgacacgaag
     3841 gtgccctgga ccacggagat gaccgcgccc gaccagacct cccgctcgcc ccgcttggtg
     3901 aacgggctca cgagctcagc ccgcgggctg gctgacggcc tcgggctcga acaggtgcgg
     3961 gcgccgaccc tcggaacgct ccagcgcggc gccgacgaac tcccggaaca gcggggccgg
     4021 gcggttggga cggctcttca gctccggatg ggcctgggtg gcgatccaga acgggtggcc
     4081 cggcagctcg atgaactcga ccaggcgccc gtcgggggac tcgcccgagc agagcaggcc
     4141 gccttcttcg aaccgggtcc ggtaccgggg gttgaactcg tagcggtgcc ggtgacgctc
     4201 ggagacgacg tccttgccgt acgcccgggc cacctgcgag tcggggacga gcttggccac
     4261 gtaggccccg aggcgcatgg tgccgccctt gtcggtgacg tcgcgctggg cgtccatcag
     4321 gtcgatcacg gcgtgcgggg tggcggcgtc gaactcgctg gagttggccc cctcgaggcc
     4381 gagcacgtcg cgggcgtatt cgatcaccat cgtgtggagg ccgagacaca gccccaggca
     4441 ggcgatgccc tcctcacggg cgtacccggc cgcggcgatc ttgccctcga tgccccgttc
     4501 gccgaagccg ccggggatga cgatgccgtc gaggtccttc aggcgcccgg cgccgagcag
     4561 gccctcgacc tcctcggccg agatcaggtc gatctcgacc ttcgccccgt gggcgaaccc
     4621 ggcatggttg agtgactcga tgaccgagag gtaggcgtcg ggcaggctgg tgtacttgcc
     4681 gaccacgccc acccgcaccg tgcgatcggc ggcgtggacc cggtcgacca gggtctccca
     4741 ttcggtgagg tcgaccggac ggtcgtcgag ccgcaggatc tcgcagacga cggcatccat
     4801 gccctcctcg tgcaggacca ggggcacgtc gtagaggctg tcggcgtcgg cggcgttgac
     4861 cacggcgcgg acctcgacgt cacacaggtt cgagatcttg cgcttgaggt cggtggagat
     4921 ctcgtccgcg ctccggcaga cgatgacgtc gggctgaatg ccccggctgc gcagctcggt
     4981 cacggagtgc tgggtcggct tggtcttctg ctcccccgcc gggccgatga gcggcaccag
     5041 ggtgaggtgg acgtaacaca cgttgtcccg gccgacgtcg aggcggaact gccggatggc
     5101 ctcgaggaac gggaggatct cgatgtcgcc cacggtgccg ccgacctcgg tgatgacgac
     5161 gtcgacgtcg tcggtggcca gcaccttgat gcgacgcttg atctcgttgg tgatgtgcgg
     5221 gatgacctgg accgtcttgc ccaggtagtc cccccgccgc tcggcggcga gcacggactg
     5281 gtagatcgag cccgtcgtgg cgttggagtc ccgggtcagg ctctcgtcga tgaagcgctc
     5341 gtagtggccg aggtcgaggt ccgtctcacc cccgtcgtcg gtgacgtaca cctcgccgtg
     5401 ctcgaaggga ttcagggtcc ccgggtcgac gttgatgtag gggtcgagct tctggatcgt
     5461 gacccgtaac cctcgggact tgaggaggcg ccccagcgag gctgccgtga tccccttgcc
     5521 cagcgaactg gccacaccgc ccgttacgaa cacatgcttc gccacgggag atggacggta
     5581 gcatcgcccg accgggaccg ggcggacccc gctcgggaat cccccgccgc cgcccgtgac
     5641 cgacgcctcc cccgcccccg ccgacgcccc cgatcgggcg gccctcgccg agccggccac
     5701 cacccggcgg gacctcctct ggctcggtgc actgaccctg gcgctgcgcc tcccgacctt
     5761 cttcgccacc aagcacctga ccttcgacga cggcgtcttc ggcgcctcgg cggtggccat
     5821 gcgcaacggc ggcgtcccct tccgggacgt cttctccagc cagggcccac tcttcctgcc
     5881 cctggtctgg atcgccgaca ccctgggcct gcacaccctc gacgggcccc gcctgctcgg
     5941 cctcgcgtcc ggactcgccc tggtcatcgt gatctacttc gcgggccgcg agctgggcgg
     6001 gaccaccggc gcccgcatcg ccggcggcct ggcggcggtg accggcagca gcatcgccgt
     6061 caccggctcc atcgccgctg acg
//
LOCUS       DASTFV010000603         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2304887, whole genome shotgun sequence.
ACCESSION   DASTFV010000603 DASTFV010000000
VERSION     DASTFV010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2304887"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFP02_11025"
     CDS             <1..288
                     /locus_tag="VFP02_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543609.1"
                     /translation="TLVQMGVSSPARFEWTPWYFKELRLVGSNAFGVEEVDGVRKHGI
                     AHYLDLVQDGRIDLDGMLTHTFRLEQWRDALAACADQGSSGALKVAFDFRG"
     gene            298..762
                     /locus_tag="VFP02_11030"
     CDS             298..762
                     /locus_tag="VFP02_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET9543610.1"
                     /translation="MSPQIATITNVDLDGLLAFIRPRHRMVLVTHRRDGSPQLSPVTG
                     GVDGEGRIVISTYAERAKTTNARRDPTASVLVLSDEWNDAWVQVDGTAEVLDIPDAVE
                     PLVEYFRVIAGEHDDWDEYRQAMVRQGKALLRITPERWGPVATGGFPPDRAP"
     gene            773..1774
                     /locus_tag="VFP02_11035"
     CDS             773..1774
                     /locus_tag="VFP02_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9543611.1"
                     /translation="MELIDLGRSGLRVTPVGLGMASIGRPAYINLGHGEDIGADRSVA
                     AVQALAVDVLDTAWEQGVRHVDAARSYGRAEEFLAAWMEARELGRDDLTVSSKWGYEY
                     TADWLVDPPSGRHETKEHSVGHLRHQWDESHRLLGDRLALYQVHSATFDSGVLDDREV
                     FDALAAIRERDVVIGITVTGVAQRDLIRRSMEIEYDGRFLFETVQATWNLLETSAEDA
                     LAEAAHEGIGVIVKEVLANGRLTERNRLGGDGDLVEALGDAADALEVTLDAAAIAAAL
                     AQPWSHVVLSGAATPAQLKSNLQAVGAEWTDRLAKPFENLREDPTDYWRTRSFLPWT"
     gene            1794..>2384
                     /locus_tag="VFP02_11040"
     CDS             1794..>2384
                     /locus_tag="VFP02_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9543612.1"
                     /translation="MSLRPTFVHPEADRWTAPGGAWDLPPLDDVLGPSEGVAATAGGL
                     HALGVSRGDVVAWQRPNGPDVVALYRACWRLGAVAAPIHHQAGDAEAARLLARVDPTV
                     FFVADDPLPEGPPAAPTGRSADVACVLWTAGSAGEPKGVLHTQRTLGYKAKVMVDVHG
                     LTAADVVLMPAPLAHISGLLNGLLVPQAAGMRAVFMA"
BASE COUNT          329 a          885 c          846 g          324 t
ORIGIN      
        1 acgctggtcc agatgggtgt ctcgtcgccg gcccgcttcg agtggacgcc gtggtacttc
       61 aaggagctgc gcctggtcgg ctccaacgcc ttcggcgtgg aggaggtcga cggtgtccgc
      121 aagcacggca tcgcccacta cctcgacctg gttcaggacg gccgcatcga cctcgacggg
      181 atgctcaccc acaccttccg cctcgagcag tggcgcgacg ccttggccgc ctgtgccgac
      241 cagggctcct cgggggcgct gaaggtcgcg ttcgacttcc gtgggtaagg aggggccatg
      301 agcccgcaga tcgccaccat caccaacgtc gacctcgacg gcctgctcgc cttcattcgg
      361 ccccggcacc gcatggtgct ggtgacgcac cgccgcgacg gctcgcccca gctgtccccc
      421 gtgacgggcg gtgtggacgg cgagggccgg atcgtcatct ccacctacgc cgagcgggcc
      481 aagacgacca acgcccggcg ggacccgacc gcctcggtgc tggtgctctc cgacgagtgg
      541 aacgacgcct gggtgcaggt cgacggcacg gctgaggtgc tcgacatccc cgacgccgtc
      601 gagccgctgg tcgagtactt ccgggtcatc gccggcgagc atgacgactg ggacgagtac
      661 cggcaggcca tggtccgcca gggcaaggcc ctgctgcgca tcaccccgga gcggtggggc
      721 ccggtcgcca ccggcggctt cccgcccgac cgcgccccgt agcgtcacgc ccgtggagct
      781 gatcgacctc gggcggagcg ggttacgggt cacccccgtc gggctgggca tggcgtcgat
      841 cgggcggccg gcgtacatca acctcggcca cggagaagac atcggcgcgg atcgcagcgt
      901 ggccgcggtc caggccctgg ccgtcgacgt gctcgacacc gcgtgggaac agggcgtccg
      961 ccacgtcgac gccgcccgct cctacgggcg agccgaggag ttcctggctg cctggatgga
     1021 ggcccgggag ctcggccggg acgacctgac cgtgagctcc aagtggggct acgagtacac
     1081 ggccgactgg ctggtcgacc cgcccagcgg ccggcacgag accaaggagc actccgtcgg
     1141 ccacctccgg caccagtggg acgagagcca ccgcctgctc ggcgaccggc tggcgctcta
     1201 ccaggtccac tcggccacct tcgacagcgg cgtgctcgac gaccgggagg tcttcgacgc
     1261 cctggccgcc atccgggagc gggacgtggt catcggcatc acggtcacgg gcgtggccca
     1321 acgggacctg atccgccggt cgatggagat cgagtacgac gggcgcttcc tgttcgagac
     1381 cgtccaggcg acgtggaacc tgctggagac gtcggccgag gacgccctgg ccgaggcggc
     1441 ccacgaaggc atcggcgtga tcgtgaagga ggtgctggcc aacggccgct tgacggagcg
     1501 caaccggctc ggcggcgacg gcgacctggt cgaagccctg ggcgacgccg ccgacgccct
     1561 cgaggtcacg ctggacgccg ccgccatcgc cgccgccctg gcccagccct ggagccatgt
     1621 cgtgctcagc ggagcggcca ctcctgcgca gctcaagtcc aacctgcagg ccgtcggcgc
     1681 cgagtggacc gaccgcctgg ccaagccctt cgaaaacctc cgcgaggacc cgacggacta
     1741 ctggcgcacc cgctccttcc tcccatggac ctgatcccga acctgcggtg cccgtgagcc
     1801 tgcggccgac gttcgtgcac ccggaggcgg atcggtggac ggcgccgggt ggggcgtggg
     1861 acctgccccc gctggatgac gtgctcggtc cgtcggaggg cgtcgccgcg acggccggcg
     1921 gcctccacgc gctcggggtg agccggggcg acgtcgtggc ctggcaacgg cccaacgggc
     1981 cggacgtggt cgccctctat cgggcctgct ggcggctggg cgcagtggcc gccccgatcc
     2041 accaccaggc cggcgacgcc gaggcggctc gcctgctggc ccgggtcgac ccgacggtgt
     2101 tcttcgtcgc cgacgatccc ctgcccgagg gcccgcccgc cgccccgacc ggccggtccg
     2161 ccgacgtcgc ctgcgtgctg tggaccgcgg gctcggcggg cgagcccaag ggcgtcctgc
     2221 acacccagcg cacccttggc tacaaggcca aggtcatggt cgacgtccac gggctgaccg
     2281 ccgcggacgt ggtcctcatg cccgcgccct tggcccacat cagcggcctc ctcaacggcc
     2341 tgctcgtccc gcaggccgcc ggcatgcggg cggtgttcat ggcc
//
LOCUS       DASTFV010000604         3232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2307025, whole genome shotgun sequence.
ACCESSION   DASTFV010000604 DASTFV010000000
VERSION     DASTFV010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2307025"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VFP02_11045"
     CDS             complement(<1..349)
                     /locus_tag="VFP02_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543613.1"
                     /translation="MPWLFLIAALIGFWFTLNAFRPVRRNTLFFGQSFFAGWLTTELA
                     GHHLVWQFAMTVVFVATGALDAWPGWVALAVTLVSWTGLVVLVVQGHRAARQVQDALG
                     DLLAGDPPPGIPWS"
     gene            397..639
                     /locus_tag="VFP02_11050"
     CDS             397..639
                     /locus_tag="VFP02_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543614.1"
                     /translation="MRAPVQTSSRPRRRALGLTVALLTLAGVAVTSCSDDGPSSPAIS
                     GNSPSDVSVPTVPTTTETTVPGTTAGTDTTTTTAAP"
     gene            complement(672..1838)
                     /locus_tag="VFP02_11055"
     CDS             complement(672..1838)
                     /locus_tag="VFP02_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9543615.1"
                     /translation="MPTDPALAEELVATVRRWVAKEVIPNASEFEHADEFPEAMVQQM
                     REFGLFGATIPEEHGGLDLDVLTYARLVEELAYGWMSLSGVLNTHTIVANLVKRFGTD
                     EQRAHWLPAMATGEIRGAFSLSEPDAGSDAQALRCKATPDGDEYIVNGTKMWVTNGER
                     AEVVALVARTPEGITCFIIGKEPGPEFEGISTSRRIDKLGYKGLETVEMAYDGHRIGA
                     DTVLGGPDNLGKGMHQVLSSLELGRINIAARANGVSRAAFDAALAYAQQRETFGVPIA
                     KHQAIQFKLADMATKLQAAKLLVKNAAEKFEAGERADVEAGMAKLFCSETALEIATEC
                     MRIHGGMGYTAELPVERYYRDAPLMIIGEGTNEIQRVVIARGLLRRAEAGEPVL"
     gene            1881..2522
                     /locus_tag="VFP02_11060"
     CDS             1881..2522
                     /locus_tag="VFP02_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543616.1"
                     /translation="MEDRRTPGLYLELADAAPGPYGTDRAPTVAAGTGITRASWWSNQ
                     HPDRDDVPRRLAEFTTLGVYELSDGFTPPTAPEGIAGLHFAATPRPGQGVLGGDATNG
                     LLLVLISPRTPDEGQDLRDWGDFVHIRHIAEANVPGYRMITPYEQVGGGEPRYLHFYE
                     MATDDPETTYKSMTPLVQARLGPPGTPAYDDWAWHPALRIDYVNTFRLEGELA"
     gene            complement(2529..2972)
                     /locus_tag="VFP02_11065"
     CDS             complement(2529..2972)
                     /locus_tag="VFP02_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543617.1"
                     /translation="MRRGVVVLTAVVAVVALPGCGGDDGGAADVSTDAGVTTATAATV
                     TTVPAGEPVTAAEAATLPDGTTVTVLAYAFEPDEGATVLCDLLGESFPPTCVGPQLVT
                     NGMDINSLPGVETTAPGDFVAPATWTARPVEVTGTLADGALQVDP"
     gene            complement(2977..>3232)
                     /locus_tag="VFP02_11070"
     CDS             complement(2977..>3232)
                     /locus_tag="VFP02_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543618.1"
                     /translation="MSGRITKSDARMAYYPMDTFGGQSGSPVFNNRAPSAPGPCSGPC
                     GMAVHSYGVGLPGAAHLNAGPRLTAARIGQILAVADDNGT"
BASE COUNT          480 a         1146 c         1144 g          462 t
ORIGIN      
        1 agctccaggg gatgccggga ggtggatcgc ccgccagcag gtcaccgagc gcgtcctgga
       61 cctgccgggc cgcccggtga ccctggacca ccaggaccac cagccccgtc cacgacacga
      121 gggtcaccgc cagggcgacc cagccgggcc aggcgtcgag cgcgccggtg gccacgaaca
      181 ccacggtcat cgcgaattgc cagaccaggt gatggccggc cagctcggtg gtgagccagc
      241 cggcgaagaa gctctggccg aagaagaggg tgttgcgccg caccggtcgg aacgcgttca
      301 gcgtgaacca gaaaccgatg agcgcagcga tcaggaagag ccaaggcacg tcatcagtct
      361 ggcgtcaccc ccgcccgaag tggtagtccc acggacgtgc gcgcacccgt tcagacctct
      421 tcgcggcccc gccgccgggc tttgggcctc accgtggccc tcctcaccct cgccggggtg
      481 gccgtcacga gctgcagcga cgacgggccg tcctcgcccg cgatcagcgg caacagcccc
      541 agtgacgtgt ccgtgccgac cgtgccgacg accaccgaga ccacggtgcc cggcacgacc
      601 gcggggacgg acaccaccac cacgaccgcc gccccctagc ggcccgagtc gggctcagac
      661 ccgacccagg atcagaggac gggctcgccc gcctctgctc gtcggagcag gccgcgggcg
      721 atgacgaccc gttggatctc gttggtgccc tcgccgatga tcatcagcgg ggcgtcccgg
      781 tagtagcgct cgacgggaag ctcggccgtg tagcccatgc cgccgtggat gcgcatgcac
      841 tcggtggcga tctcgagggc cgtctccgag cagaagagct tggccatgcc ggcctcgacg
      901 tccgcccgct cgccggcctc gaacttctcg gcggcgttct tcaccagcag cttggcggcc
      961 tggagcttgg tggccatgtc ggccagcttg aactggatgg cctggtgctt ggcgatgggc
     1021 acgccgaagg tctcccgctg ctgggcgtag gccagcgcgg cgtcgaacgc ggcccggctc
     1081 acgccgttgg cgcgggcggc gatgttgatc cggcccagct ccagcgagct gaggacctgg
     1141 tgcatgccct tcccgaggtt gtccgggccg ccgaggaccg tgtcggcccc gatgcggtgg
     1201 ccgtcgtagg ccatctcgac cgtctccagg cccttgtagc cgagcttgtc gatgcgacga
     1261 ctggtcgaga tgccctcgaa ctcggggccc ggctccttgc cgatgatgaa gcaggtgatg
     1321 ccttcggggg tcctggccac cagggccacg acctcggccc gctccccgtt ggtcacccac
     1381 atcttggtgc cgttgacgat gtactcgtcg ccgtcgggcg tggctttgca gcggagggcc
     1441 tgggcgtcgc tgccggcgtc gggctcggac aacgagaagg cgccccggat ctctccggtg
     1501 gccatggccg gcagccagtg cgctcgctgc tcgtcggtgc cgaagcgctt gaccaggttg
     1561 gccacgatgg tgtgggtgtt gaggacgccg gacagggaca tccagccgta ggccagctcc
     1621 tcgacgaggc gggcgtaggt caggacgtcg aggtcgaggc cgccgtgctc ctccgggatg
     1681 gtggccccga agaggccgaa ctcgcgcatc tgctggacca tggcttcggg gaactcatcg
     1741 gcgtgctcga actcggaggc gttggggatg acctccttgg ccacccaacg gcggacggtg
     1801 gcgacgagtt cttcagcgag ggcagggtcc gtgggcacgg cggcacatta ggttccgggg
     1861 cgcgtctagg ttccatcgac gtggaggacc gccggactcc cggcctgtac ctggagttgg
     1921 ccgacgccgc ccccggcccc tacggcactg atcgagcccc gaccgtcgcg gccgggaccg
     1981 gcatcacccg ggcctcctgg tggtccaacc agcaccccga tcgggacgac gtcccccgtc
     2041 ggttggccga gttcaccacc ctcggcgtct acgagctgtc cgacggcttc accccaccga
     2101 cggcccccga aggcatcgcc gggttgcact tcgcggccac gcctcggccg ggccaggggg
     2161 tcctcggcgg cgatgccacg aacggcctcc tcctcgtcct gatcagccct cggacaccgg
     2221 atgagggcca ggacctgcgg gactggggcg acttcgtcca catccgacac atcgccgagg
     2281 ccaacgtccc cggctaccgg atgatcacgc cctacgagca ggtgggcggc ggcgagcccc
     2341 gctacctgca cttctacgag atggcgaccg acgaccccga gaccacctac aagtcgatga
     2401 ctccactggt gcaggctcgc ctcggacccc cgggaacgcc cgcctacgac gactgggcgt
     2461 ggcaccccgc cctgcgcatc gactacgtca acaccttccg cctcgaaggc gagctggcct
     2521 gactcgggtt acggatcgac ctggagggcg ccgtcggcga gggtgccggt gacctcgacg
     2581 gggcgggcgg tccaggtggc cggggccacg aagtcgccgg gggccgtggt ctccacaccc
     2641 gggaggctgt tgatgtccat cccgttggtc accagctgcg gcccgacgca ggtcggcggg
     2701 aacgactcgc ccagcaggtc acagaggacg gtggccccct cgtcgggctc gaacgcatag
     2761 gccagcacgg tgaccgtggt gccgtcgggc agggtggcgg cctcggccgc ggtcacgggc
     2821 tcgccggcgg gaaccgtcgt caccgtggcc gccgtggcgg tggtgacccc ggcgtcggtg
     2881 ctgacgtcgg cggccccgcc gtcgtcaccg ccgcagcccg gcagggccac gacagcaacg
     2941 acggcggtga ggacgacgac gccccgccgc aagcgatcag gtcccgttgt cgtcggcgac
     3001 ggccaggatc tgtccaatgc gggcggcggt gagccgcggc ccggcgttga ggtgcgcggc
     3061 gccgggcaac cccacgccgt aggaatggac ggccatccca caagggcccg agcagggtcc
     3121 cggggcgctg ggggcacggt tgttgaagac gggcgagccg gactgcccac cgaacgtatc
     3181 catcgggtag taggccatcc gggcatcgga cttggtgatc cggccggaca tc
//
LOCUS       DASTFV010000605         3770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2307152, whole genome shotgun sequence.
ACCESSION   DASTFV010000605 DASTFV010000000
VERSION     DASTFV010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2307152"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1255)
                     /locus_tag="VFP02_11075"
     CDS             complement(65..1255)
                     /locus_tag="VFP02_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9543619.1"
                     /translation="MTDFPPDDQVPVADSLPPHPPSMFSGRPPDLPPVPGADPPAGRP
                     RRWRLPLAIVAGLALLVGAFAGGWALNRGDDSGNDAASAAANRSSSIQVAADVEDRPT
                     EPPLDSSDEEPAAAVADAVAPAVVQIETAQGLGSGVIYDPDGLILTAAHVVAGSEHVT
                     LRLADGTTRDGTVVGTDTDSDIGVVSIDGGGDGLPVAVLADSPPDVGDLAVAVGSPYA
                     LDQTVTAGIVSALDRPAPSGGPSVGTIQTDAPINPGNSGGPLANREGEVIGIASYIQS
                     ETGGNVGLGFAVPVDMAERVADAIVAGEPVEFGYLGIEGGDAAGSDPGALLASIVPGS
                     PAEDAGLQAGDRVVAVDGDAVTSFGDFGVIIRRHGPGDELALTVARNGDEQEITVTLG
                     STAD"
     gene            complement(1303..2526)
                     /locus_tag="VFP02_11080"
     CDS             complement(1303..2526)
                     /locus_tag="VFP02_11080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9543620.1"
                     /translation="MTTARTAVGLASRLPPWAGSIRVRLTVLYAVLVFGLSALLTGGV
                     YLGLTRSLSDEPVSRTFNLTRVVRTPQGVVEVPQGTVRAEIQGLEAEVNDRALDELRR
                     WSLLALTAGAVAGVGVGWFAAGRVLRPVDRITGVARDIQATDLSRRIALDGPDDEMKE
                     LADTFDGMLGRLDEAFEGQRHFFQEASHELRNPLAVIRTNLDVALADEGASAPELRTA
                     AELAARTAERMTHTVDDLLRYARNEQAVMAREDIDLGLLAAEVADEFRSPAATRSMHI
                     ETAMPSGPVVVSGDRAALRQAVANLLDNAVRLAPEGSTIQVGAGLDGPWSWIAVGDEG
                     PGITAEDQPLVFRRFWRGASVDGPRGSGLGLAIVDQILVAHGGAARVVSEPGRGSVFS
                     LWIPRHPVEVDAAEG"
     gene            complement(2523..3215)
                     /locus_tag="VFP02_11085"
     CDS             complement(2523..3215)
                     /locus_tag="VFP02_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9543621.1"
                     /translation="MRLLVVDDEVDLADAVATGLRREGHAVDVAYDGDAALELLAVDE
                     YDLVLLDVAMPGDHDGLAVCRAIRDGDAAGPMPRVLMLTARDTVDDRVTGLDAGADDY
                     LVKPFAFRELNARVRTLLRRDAAPTGAVVEVGDLRLDTARQEATRSGVALDLTPKELS
                     VLHWFLTRPGVVVSQEELLTHVWDANADPFTSTVRVTVGTLRRKLEAARPDAEGPLET
                     LVGQGYRLRDDR"
     gene            3305..>3770
                     /locus_tag="VFP02_11090"
     CDS             3305..>3770
                     /locus_tag="VFP02_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9543622.1"
                     /translation="MTQAPLPPPPTGAPVAPGPAPGPEDVPTRAALLEKALFEVKKVI
                     VGQDQAIERLLTCLLARGHVLLEGVPGVAKTLASETLANVVGGSFVRIQFTPDLLPAD
                     IVGTRIYRGSSESFDVELGPVFVNFLLADEINRAPAKVQSALLEIMAEQQVSI"
BASE COUNT          523 a         1381 c         1385 g          481 t
ORIGIN      
        1 acggccagct tcggcggagc cggacttgct gagcgcagcg aagccagtca gatgtaacga
       61 ctactcagtc ggcggtggat ccgagggtga cggtgatctc ctgctcgtcg ccgttgcggg
      121 cgacggtgag ggccagctcg tcgccggggc cgtggcggcg gatgatcacg ccgaagtcac
      181 cgaagctggt gacggcgtcg ccgtccacgg ccacgacccg gtcaccggcc tggaggccgg
      241 cgtcctcggc gggcgatccg ggcacgatcg acgccagcag ggccccgggg tcgctcccgg
      301 ccgcatcgcc cccttcgatc ccgaggtagc cgaactcgac cggctcgccg gcgacgatgg
      361 cgtcggccac ccgctcggcc atgtccaccg gcacggcgaa cccgaggccg acgttgccgc
      421 cggtctcgct ctggatgtag ctggcgatgc cgatgacctc gccctcccgg ttggccaggg
      481 ggccgcccga gttgcccggg ttgatcggag cgtcggtctg gatggtgccc accgacgggc
      541 ccccggaggg cgccgggcgg tcgagggcgc tgacgatgcc cgcggtcacg gtctggtcca
      601 gcgcgtaggg gctgcccacg gcgacggcga ggtcaccgac gtccggcggg gagtcagcca
      661 ggacggcgac cggcaggccg tcgccaccac cgtcgatcga gaccacgccg atgtcgctgt
      721 ccgtgtcggt accgaccacg gtgccgtccc gggtcgtgcc gtcggcgagg cgcaacgtca
      781 cgtgctcact gcccgcgacc acgtgggccg cggtgaggat caggccgtcc gggtcgtaga
      841 tgacgccgga cccgaggccc tgggcggtct cgatctgcac gacggccggg gcgaccgcgt
      901 cggccaccgc cgcggccggc tcctcgtccg acgaatccag cgggggctcg gtgggacggt
      961 cttcgacatc ggccgccacc tggatcgacg acgatcggtt ggcggctgcc gaggcggcgt
     1021 cgttgccgct gtcgtcaccc cggttcaggg cccaaccgcc ggcgaaggcg ccgaccagca
     1081 gggccaggcc cgcgacgatg gcgaggggga ggcgccaccg tcggggccgg cctgccggcg
     1141 ggtcggcacc ggggacggga ggaaggtccg ggggtcgccc cgagaacatc gagggcgggt
     1201 gcgggggaag gctgtcggcc acgggtacct gatcgtcggg ggggaagtcg gtcacatcgg
     1261 ggaaggtacg gagtcaaccc gaaaatggat cgaaaggccc gctcagccct cggcggcgtc
     1321 gacctcgacg gggtggcgcg ggatccacag cgagaagacc gagccccgcc ccggctcgga
     1381 gacgactcgg gcggcgcccc cgtgggcgac caggatctgg tcgacgatgg ccaggccgag
     1441 cccgctgccc cggggcccgt cgacggaggc tcccctccag aagcgccgga acaccagcgg
     1501 ttggtcctcg gcggtgatgc cgggaccttc gtcaccgacc gcgatccagc tccacgggcc
     1561 gtccagcccg gcccccacct ggatggtcga gccttcgggg gccaggcgca cggcgttgtc
     1621 cagcaggttg gccacggcct ggcgcagagc cgcccggtcc ccggagacga cgaccggccc
     1681 cgacggcatc gccgtctcga tgtgcatcga ccgggtcgca gccggcgagc ggaactcgtc
     1741 ggccacctcg gcggcgagaa gcccgagatc gatgtcctcc cgagccatga cggcctgctc
     1801 gttgcgggcg taccgcagga ggtcgtcgac ggtgtgggtc atgcgctcgg cggtgcgggc
     1861 cgccagctcg gctgcggtgc gcagctccgg agccgaggcc ccctcgtcgg ccagggccac
     1921 gtcgaggttg gtgcggatca ccgccagcgg gttgcgcagc tcgtgggacg cctcctggaa
     1981 gaagtggcgc tggccctcga aggcctcgtc gagacgcccg agcatcccgt cgaaggtgtc
     2041 ggccagttcc ttcatctcgt cgtcggggcc gtcgagggcg atgcggcggg agaggtccgt
     2101 ggcctggatg tcccgggcca cgccggtgat ccggtcgacg gggcgcagga cccgtccggc
     2161 cgcgaaccac cccaccccga cgccggcgac ggcgccggcc gtgagcgcca gcagggacca
     2221 gcggcggagc tcgtccagcg cccggtcgtt gacctcggcc tccaagccct ggatctcggc
     2281 ccgcaccgtc ccctgcggca cctcgacgac cccctggggc gtgcgcacga cccgggtcag
     2341 gttgaaggtc cgggagacgg gctcgtcgga gagcgagcgg gtcaacccca ggtagacccc
     2401 gccggtcagc agggccgaga gcccgaacac gagcacggcg tacagcacgg tcaggcgcac
     2461 ccggatcgac cccgcccacg gagggagccg ggaggcgagg cccaccgccg tcctggccgt
     2521 ggtcaccggt cgtcccgcag ccggtagccc tgcccgacca gcgtctccag cggtccctcc
     2581 gcatcgggcc gggcggcctc cagcttccgg cgcaacgtcc cgaccgtcac ccgcaccgtg
     2641 ctggtgaagg ggtcggcgtt ggcgtcccac acgtgggtca gcagctcctc ctgggacacg
     2701 accacaccgg ggcgggtgag gaaccagtgc aggaccgaca actccttcgg ggtcaggtcc
     2761 agggccacgc ccgagcgggt cgcctcctgg cgggccgtgt ccaaccgcag gtcgccgacc
     2821 tcgacgacgg cgccggtcgg cgcggcgtct cgccgcagca gggtgcgcac gcgggcgttc
     2881 agctcccgga aggcgaacgg cttgaccagg tagtcgtcag cgccggcgtc gagcccggtg
     2941 acccggtcgt caaccgtgtc ccgggccgtg agcatcagga cccggggcat cgggccggcc
     3001 gcgtcgccgt cccggatggc ccggcacacg gcgaggccgt cgtggtcgcc gggcatggcc
     3061 acatcgagca gcacgaggtc gtattcgtcg acggccagga gctcgagggc cgcgtccccg
     3121 tcgtaggcga cgtcgacggc atggccttcg cgccgtagcc ccgtggccac ggcatcggcc
     3181 aggtcgacct cgtcgtccac gaccaacagg cgcacgccgg cgacggtacc gccgggtcag
     3241 gccgccgtac cggcagaccc gggcgcgcgt cggaggcggc agcccacccg tatcgtggcc
     3301 gcccgtgacc caggctcccc tgcccccacc cccgaccggt gcacccgtgg cgccgggtcc
     3361 cgccccgggc ccggaagacg tccccacccg ggccgccctc ctcgagaagg cgctcttcga
     3421 ggtgaagaag gtcatcgtgg gccaggacca ggccatcgag cgcctgctca cctgcctcct
     3481 ggcccgaggc cacgtgctgc tcgagggcgt gcccggcgta gccaagacgt tggccagcga
     3541 gaccttggcc aacgtcgtcg gtggctcctt cgtccgcatc cagttcaccc ccgacctcct
     3601 tcccgccgac atcgtcggca cccgcatcta ccgcggctcg tcggagagct tcgacgtcga
     3661 gctcggcccg gtgttcgtca acttcctgct cgccgacgag atcaaccgcg cacccgccaa
     3721 ggtgcagtcg gcgctgctgg agatcatggc cgagcagcag gtgtcgatcg
//
LOCUS       DASTFV010000606         5644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2307722, whole genome shotgun sequence.
ACCESSION   DASTFV010000606 DASTFV010000000
VERSION     DASTFV010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2307722"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VFP02_11095"
     CDS             complement(<1..796)
                     /locus_tag="VFP02_11095"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HET9543623.1"
                     /translation="MSSHSAVDVIRTKRDGGRLSDDQIRWFMTAYTAGEVADEQAAAL
                     AMAIFWRGMEPDELAVWTRAMIESGERLDLSAITRPTVDKHSTGGVGDKVSLPLIPLA
                     AACGLAVPQLSGRGLGHTGGTLDKMEAIPGWRAQLSNEEFIAVLAAEGGVICAAGPGL
                     APADRRLYALRDTTGTVESIPLIASSIMSKKIAEGTDALVLDVKFGSGAFLPDPDRGR
                     ELARTMVGLGRAHGVRTTALLTAMEQPLGRTAGNAVEVEESVEVLAG"
     gene            complement(810..1196)
                     /locus_tag="VFP02_11100"
     CDS             complement(810..1196)
                     /locus_tag="VFP02_11100"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET9543624.1"
                     /translation="MDIDWDALRTAAHEVGEAAYAPYSRLRVGAAALTDGGAIVTGCN
                     VENASYGLTLCAECGLASALAAAGGGRLVALSCVAPDRDERLMPCGRCRQVLYEFGGA
                     ELLVDGPNGPLPLGDLLAHAFGPDDL"
     gene            complement(1200..2519)
                     /locus_tag="VFP02_11105"
     CDS             complement(1200..2519)
                     /locus_tag="VFP02_11105"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9543625.1"
                     /translation="MSLEGMPSVAGEGAVSRADQLTAASRLRSARKRQKALAIGGGLF
                     LLMAAVRWLTDSPDLTSSGTFGTALRLAVPIGLAALGGLWAERSGIVNIGLEGMMILG
                     TWFGAWGAWQYGPWQGVAIGIAGGVAAGLLHALATVTFNVDHIISGVAIITLASGIVR
                     YLSVEVYTGVAGGGATQSPRVEGVGTFDLPILSGGELFGWNSPDSLGWLEEHHWLVVS
                     DLAGLLRGFTHNLSYLTLLALILVPVTWWVMWRTVFGLRVRSCGEHPTAADSLGVPVN
                     RMRYLALMISGGLAGLAGAFLAIEAAGIYRQGQTQGKGFIGLAALIFGNWLPFGAAAG
                     AGLFGFADALNLRDKSAVHALLLFIAVAALVYAVVQFRNGKRRAALVTGLLGVLSGLW
                     FATTDEVPVEFVQMTPYVVTLLVLAVATQRLRPPAADGKPWRKGQVE"
     gene            complement(2516..3631)
                     /locus_tag="VFP02_11110"
     CDS             complement(2516..3631)
                     /locus_tag="VFP02_11110"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9543626.1"
                     /translation="MRRLYVPLLRLLAPLAAVLFALTVSGLVLKLSGNDPFHAFKLMW
                     DFGTTEQSIASIVDRSVPLYLSGVAFAVAFKMGLFNIGVEGQYTIAVLMAAFVGAAMN
                     LPPVLHVTLILVVAMLSGMVWGAIPGVLKVTRGVSEVISTIMLNSIATFLVSYLLAHH
                     FLDRADETLNLQTKKIPESGWFPDLTKLGPNATINAIVLLALAVGIGYYVLIWRTRFG
                     YDLRASGLNPLAAQASGVAPRAMIVKGMMLSGAVAGLVGMPTLLGFSHNYGLDFGTGL
                     GFAGITVALLGRNNPIGIAVGALLLAFLDRSAQILDLEDIAKEIVVIMEGVIVLSVVI
                     AYELVQRVIERQQRRMIGEDVPPPAPTVVAEPAAAPA"
     gene            complement(3631..5196)
                     /locus_tag="VFP02_11115"
     CDS             complement(3631..5196)
                     /locus_tag="VFP02_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9543627.1"
                     /translation="MTGSDEARRRAVDTDQATAVELEGIVKRFPGVVANDGVDLAVRR
                     GAIHALVGENGAGKSTLMKILYGMQPPDEGTMSVNGQPVAFRSPKDAISAGIGMVHQH
                     FMLADQLTVLENIVLGDEPSQVGRIDFKEARERIQKLSSAYGLDVDPNQTVETLGVGE
                     RQRVEIIKVLFRGARLLILDEPTAVLVPQEIEELFDNLRELKAQGETIVFISHKLDEV
                     LAIADTITVLRAGRTVATVDPHDVTAGDLAELMVGSELPTPETRESTVTEEVGLEVDD
                     VSVQGEGDRWLLEHIAFKVHRGEVVGVAGVEGNGQTELIDVILGLQPATTGAIRLGDE
                     DLVAVGTRARREAGIGYIPEDRHRRGMLMSSPLWENAMLGHQTQPPYAKGPWIDRAGA
                     KRRTEEILDTYDVRAPGFDVAGYALSGGNQQKLIVGRELTAGAKVLLANHPTRGVDVG
                     AQAAVWDHLRDARAAGLATLLVSADLEELIGLSDRLLVMLRGRIVTELDPATVTPEEL
                     GSYMTGARTGEAA"
     gene            complement(5288..>5644)
                     /locus_tag="VFP02_11120"
     CDS             complement(5288..>5644)
                     /locus_tag="VFP02_11120"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9543628.1"
                     /translation="AGGSGSGVFEAAQAVRDAGGDAWAIGVDSDQYNTADPAVQDVIL
                     TSMLKQVNVAVYNTIASAVAGDEVGGQTTVFDLSVDGVGYSTSGDFLSEETITALEDY
                     KQQIIDGGIEVPTVPE"
BASE COUNT          857 a         1951 c         2043 g          793 t
ORIGIN      
        1 ccccggccag cacctcgacg gactcctcga cctcgacggc attgcccgcc gtgcgcccca
       61 gaggctgttc catggctgtg agcagcgctg tggtccgcac gccgtgggcg cggcccaatc
      121 cgaccatcgt gcgagccagc tcccggcccc gatcgggatc ggggagaaag gcgcccgagc
      181 cgaacttcac atcgaggacc agggcgtcgg tgccctcggc gatcttcttg ctcatgatcg
      241 acgaggcgat cagggggatg gactcgaccg tgccggtcgt gtcccgcagg gcgtacaggc
      301 ggcggtcggc cggagcgagg ccgggcccgg cggcgcagat gaccccaccc tccgcggcca
      361 gcaccgcgat gaactcctcg ttgctgagct gggcccgcca gccgggaatg gcctccatct
      421 tgtccagggt cccgccggtg tgacccaggc cccgacctga gagctggggc acggccaatc
      481 cgcaggcggc ggccagcggg atgaggggca gcgagacctt gtcgccgaca ccgccggtgg
      541 agtgcttgtc gacggtcggt cgggtgatgg ccgacaggtc gaggcgctcg cccgactcga
      601 tcatggcccg ggtccagacg gccagctcgt cgggttccat cccccgccag aagatggcca
      661 tggccagcgc cgccgcctgc tcgtcggcga cctcgccggc cgtgtacgcc gtcatgaacc
      721 agcggatctg gtcgtcggac aggcggccgc cgtcccgctt ggtgcggatg acgtcgacgg
      781 ccgaatggct gctcaccgga cggccacctt cagaggtcgt cggggccgaa ggcgtgggcc
      841 agcaggtcgc ccaggggcag cggcccgttc gggccgtcga ccagcagctc ggcgccgccg
      901 aactcgtaga gcacctgccg gcagcggcca cacggcatga gccgctcgtc ccggtcgggg
      961 gccacgcacg agagcgcgac cagccgcccg ccgccggccg cggcgagggc cgaggccagc
     1021 ccgcactcgg cgcacagggt cagcccgtac gacgcgttct ccacgttgca gccggtgacg
     1081 atcgccccgc cgtcggtcag tgccgcagcc ccgacccgga gacgcgagta cggggcgtag
     1141 gccgcttcac caacctcgtg ggccgccgtc cgcagggcgt cccagtcgat gtccatccgc
     1201 tactcgacct ggcccttgcg ccagggcttg ccgtcggcgg cgggcgggcg cagccgctgc
     1261 gtggccacgg cgaggacgag cagggtgacc acgtaggggg tcatctgcac gaactcgacc
     1321 ggcacctcgt cggtcgtggc gaaccagagg ccggacagca cgcccagcaa ccccgtcacc
     1381 agtgcggccc gccgcttgcc gttgcggaac tggacgacgg cgtagaccag ggcggccacc
     1441 gcgatgaaca ggagcagggc gtggaccgcg ctcttgtccc gcaggttgag ggcgtcggcg
     1501 aagccgaaca gcccggcgcc ggcggcggcg ccgaagggga gccagttgcc gaagatgagg
     1561 gcggcgaggc cgatgaaccc cttgccctgg gtctggccct ggcggtagat gccggcggcc
     1621 tcgatggcca ggaacgcccc ggccagtccc gccagccccc cgctgatcat cagggcgagg
     1681 tagcgcatgc ggttgacggg gacgcccagg gagtccgctg ccgtggggtg ctccccgcac
     1741 gagcggaccc ggaggccgaa gaccgtgcgc cacatgaccc accaggtgac gggcacgagg
     1801 atgagggcca gcagggtcag gtaggagagg ttgtgggtga acccccgcag caggccggcc
     1861 aggtcggaga ccaccagcca gtggtgttct tccagccagc cgaggctgtc gggcgagttc
     1921 cagccgaaga gctcgccgcc cgagaggatc gggaggtcga aggtccccac cccctcgacc
     1981 cgcggcgact gcgttgctcc gccgccggcg acccccgtgt agacctccac cgacaggtag
     2041 cggacgatgc cgctggccag ggtgatgatg gccacgccgg agatgatgtg gtcgacgttg
     2101 aacgtgaccg tggccagggc gtggaggagg ccggcggcga cgcccccggc gatgccgatg
     2161 gccacgccct gccagggccc gtactgccag gccccccatg cgccgaacca ggtcccgagg
     2221 atcatcatcc cctcgaggcc gatgttcacg atgcccgagc gctcggccca gagcccgccg
     2281 agggcggcca gcccgatggg cacggccagg cggagggcgg tgccgaacgt gccgctggag
     2341 gtcaggtccg ggctgtcggt gagccaccgg accgcggcca tgaggaggaa caacccgccg
     2401 ccgatggcca gcgccttctg gcgcttccgg gcgctccgga gccgagaggc cgcggtgagc
     2461 tggtcggcgc ggctcacggc accttcgccg gcgaccgacg gcatcccctc gaggctcatg
     2521 ccggtgccgc cgcgggctcg gcgaccaccg tgggggcggg cggtgggacg tcctcaccga
     2581 tcatccgccg ctgttgcctc tcgatgacgc gctgcacgag ctcgtaggcg atgaccacgg
     2641 acaggacgat gaccccctcc atgatcacca cgatctcctt ggcgatgtcc tcgaggtcga
     2701 ggatctgggc gctgcggtcg aggaaggcca gcaggagggc gccgaccgcg atgccgatcg
     2761 ggttgttgcg cccgagcagg gccacggtga tgccggcgaa cccgagcccg gtgccgaagt
     2821 ccagcccgta gttgtgggag aagccgagca gggtgggcat gccgaccagt ccggcaaccg
     2881 ccccggagag catcatcccc ttgacgatca ttgcccgcgg ggccacgccg ctggcctggg
     2941 cggcgagggg gttgaggccg ctggcccgga ggtcgtaccc gaagcgagtg cgccagatca
     3001 ggacgtagta cccgatgccg acggccaggg cgaggagcac gatggcgttg atcgtggcgt
     3061 tgggcccgag cttggtcagg tcggggaacc agcccgactc cgggatcttc ttggtctgga
     3121 ggttgagggt ctcgtcggcc cggtcgagga aatggtgggc cagcaggtag ctgaccagga
     3181 aggtggcgat ggagttgagc atgatcgtgg agatcacctc actcacgccc cgggtcacct
     3241 tgagcacccc ggggatcgct ccccagacca tcccgctgag catggccacg accaggatga
     3301 gggtgacgtg caggaccggg ggcaggttca tggccgcccc cacaaaggcg gccatgagga
     3361 cggcgatcgt gtactggccc tcgacgccga tgttgaacag ccccatcttg aaggcgacgg
     3421 cgaaggccac gccggagagg tagaggggga cgctgcggtc gacgatggag gcgatcgact
     3481 gctcggtggt gccgaagtcc cacatgagct tgaaggcgtg gaaggggtcg ttgccgctga
     3541 gcttgaggac caggcccgac acggtgaggg cgaagagcac ggccgcgagc ggcgccagca
     3601 ggcgcaggag ggggacgtac aagcgccgca tcaggcggcc tcgcccgtgc gggcgccggt
     3661 catgtacgag cccagctcct cgggcgtcac cgtcgccggg tccagctcgg tcacgatgcg
     3721 gccccgcaac atgaccagga gccggtcgga cagcccgatc agctcctcga ggtccgccga
     3781 caccaacagg gtggccagcc ccgcggcccg ggcgtcgcgg aggtggtccc agaccgcggc
     3841 ctgggcgccc acgtcgacgc cccgggtcgg gtggttcgcg agcaggacct tcgcccccgc
     3901 cgtcagctca cggcccacga tcagcttctg ctggttgcca ccggacaggg cgtaaccggc
     3961 gacgtcgaag ccgggggccc gcacgtcgta ggtgtccagg atctcctcgg tgcggcgctt
     4021 ggccccggcc cggtcgatcc acggcccctt ggcgtagggc ggctgggtct ggtggccgag
     4081 catcgcgttc tcccagaggg gcgagctcat cagcatcccg cgccggtggc ggtcctcggg
     4141 gatgtagccg atcccggctt cacggcgggc ccgggtgccg accgcgacca ggtcctcgtc
     4201 gccgaggcgg atggcgcccg tggtggcggg ttggaggccg aggatcacgt cgatgagctc
     4261 ggtctggccg ttgccctcca ccccggccac cccgaccacc tcaccccggt ggaccttgaa
     4321 ggcgatgtgc tccagcagcc accggtcgcc ctcaccctgg accgagacgt cgtcgacctc
     4381 gaggccgacc tcctcggtga cggtggactc gcgcgtctcg ggggtgggca gctcgctgcc
     4441 caccatcagc tccgccaggt caccggccgt gacgtcgtgc ggatcgacgg tggccacggt
     4501 tcggccggcc cgcaggacgg tgatggtgtc ggcaatggcg agcacctcgt cgagcttgtg
     4561 ggagatgaac acgatcgtct cgccctgggc cttgagctcc cgcaggttgt cgaagagctc
     4621 ctcgatctcc tggggcacga gcaccgcggt gggctcgtcg aggatgagga ggcgggctcc
     4681 tcggaacagg accttgatga tctcgacccg ctggcgctcc ccgaccccga gggtctccac
     4741 ggtctggttg gggtcgacgt cgaggccgta ggccgacgag agcttctgga tgcgctcacg
     4801 ggcctccttg aagtcgatgc gcccgacctg cgacggctcg tcacccagga cgatgttctc
     4861 gagcacggtg agctggtcgg ccagcatgaa gtgctggtgg accatgccga tgccggcgct
     4921 gatcgcgtcc ttcggcgaac ggaaggccac gggctggccg ttgaccgaca tggtgccctc
     4981 gtccggcggc tgcatgccgt agaggatctt catcagggtg gacttgcccg cgccgttctc
     5041 gcccacgagg gcgtggatgg ccccccgccg caccgcgagg tcgacgccgt cgttggccac
     5101 gaccccgggg aagcgcttga cgatcccctc gagctcgacg gccgtggcct ggtccgtgtc
     5161 caccgccctc ctcctggcct cgtcactgcc ggtcatggat gtcgccatcg gtcgaaaacg
     5221 acgacggacc cgggcaccct agcccgggtc cgtcgcacgg ttttctggcg ggatcagccg
     5281 atccggttca ctccggaacg gtcggaacct cgatgccccc gtcgatgatc tgctgcttgt
     5341 agtcctcgag ggcggtgatg gtctcctcgg agaggaagtc accgctcgtc gagtacccga
     5401 cgccgtccac cgacaggtcg aagacggtcg tctggccacc gacctcgtca ccggcgaccg
     5461 ccgaagcgat ggtgttgtag acggccacgt tcacctgctt gagcatcgag gtcaggatca
     5521 cgtcctggac cgcggggtca gcggtgttgt actggtccga gtccaccccg atggcccacg
     5581 cgtcgccacc ggcgtcacgg acggcctggg ccgcctcgaa cacgccggag ccggaaccac
     5641 cggc
//
LOCUS       DASTFV010000607         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2320329, whole genome shotgun sequence.
ACCESSION   DASTFV010000607 DASTFV010000000
VERSION     DASTFV010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2320329"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..>2277
                     /locus_tag="VFP02_11125"
     CDS             224..>2277
                     /locus_tag="VFP02_11125"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET9543629.1"
                     /translation="MKRPTLLAAVLAVLLLPIVSATGPAEALPPSYQQGAPGIGDPYF
                     PLDGNGGYDVGAYDLELGYEPDTDVLTGLATIDLRAKRNLSRFNFDFDGLTVHSVTVD
                     GVAATFQRGNGELKITPSGGIAHGERVQVVVDYSGIPQPLPAIDGGGGWLPTDDGVLV
                     AGEPHVAAVWFPANDHPRDKARFRFEVTVPDGLEVVANGALVSQESDAGLSTWVWNAP
                     APMAPYLATVDIGEFDLTEYSDPRGRPGGIDYVDAVDTDLLTPVAPHTGTRFAFSQAA
                     PEGATFKRLRRTIAVPGGGATLSFWVNRQTETNWDFVAVEARTDGETDYTTLPDINGH
                     TSDDTGDSCPYWLGLHPRLTRYQAPSGGGCTPAGTTGVWNAASGSSDGWEQWSVDLAD
                     FAGSTAEVSISYISDDTVQGPGVAVDDIEVSTGEGTTSFETDGDELDGWQVPGAPPGS
                     DTNVNDWITGTAADGPTPAGVVAQTALARQPEMLSFLSTRFGPYPFADGGGIVDDFTG
                     LGFALENQTRPIYPPEIFGDPIGAESTIVHELAHQWYGDRVAVSAWQHIWLNEGFATY
                     AEWLWAQREGLATAQEIFDFFYSTPAGDPLWHLRVGDPGPRHLFDAPVYLRGGMTLHA
                     LRLEVGNADFFTIMRRWAAAPVGRPTTTHNFQALAERVSGRQLDTLFHEWLFTTHKPA
                     LT"
BASE COUNT          345 a          854 c          762 g          316 t
ORIGIN      
        1 gcacctgtga cgaggtctac ggcacggcgg gctacgagca gagcgtgagc aacctggccc
       61 ggacgtccat ggccaccgac atggtgttca gcgacggcgt cgaccagcag actcccacgg
      121 tgtccggtcg cgccgacgac gacctcacca tcgcgctggt cgtccccgtc tgagcccggc
      181 gcgggctctg gccgccgccc cgaggtcgcc atagtctgcg cacatgaagc gccccaccct
      241 gctcgcggcc gtcctcgccg tcctcctcct accgatcgtc agcgcgacgg gcccggccga
      301 ggccctcccg ccgtcgtacc agcagggcgc gcccggcatc ggcgacccgt acttccccct
      361 cgacggcaac gggggctacg acgtcggggc ctacgacctg gagctgggct acgagccgga
      421 caccgacgtg ctcaccggtc tggccaccat cgacctccgg gccaagcgga acctgtcccg
      481 cttcaacttc gacttcgatg gcctgaccgt gcacagcgtg accgtcgacg gcgtggccgc
      541 gacgttccag aggggcaacg gtgagctgaa gatcacgccg agcggcggga tcgcccatgg
      601 cgaacgggtc caggtcgtcg tggactacag cggcatcccg cagccgctgc ccgccatcga
      661 tggcgggggt gggtggctgc cgaccgacga cggggtcctg gtggccggcg agccccatgt
      721 ggccgccgtc tggttcccgg ccaacgacca cccccgcgac aaggcccggt tccggttcga
      781 ggtcacggtc cccgacggcc tcgaggtggt ggccaacggc gccctggtga gccaggagag
      841 cgacgccggc ctgtccacat gggtgtggaa cgcgccggcg cccatggccc cgtacctggc
      901 caccgtcgac atcggcgagt tcgacctgac cgagtacagc gatcctcggg gtcgtcccgg
      961 cgggatcgac tatgtcgacg ccgtcgacac cgacctgttg acgcccgtgg ccccgcacac
     1021 ggggacccgc ttcgccttct cgcaggccgc ccccgagggc gccaccttca agcgcctgcg
     1081 ccggacgatc gccgttcccg gtggaggggc caccctctcc ttctgggtga accggcagac
     1141 cgagaccaat tgggacttcg tcgccgtcga agcccgcacc gacggcgaga ccgactacac
     1201 cacgctgccg gacatcaacg gccacacgtc ggacgacacc ggtgactcgt gcccgtactg
     1261 gctcggcctg cacccgcgcc tgacccgcta ccaggccccc agcggcggtg ggtgcacgcc
     1321 ggcgggcacc acgggggtgt ggaacgcggc cagcggcagc agcgacggct gggagcagtg
     1381 gtccgtcgac ctggcggact tcgccgggag cacggcggag gtgtcgatca gctacatcag
     1441 cgatgacacc gtccagggcc ccggcgtggc cgtcgacgac atcgaggtgt cgaccggcga
     1501 gggcacgacg tccttcgaga ccgacggtga cgagctggac ggttggcagg tccccggcgc
     1561 gccgccgggt agcgacacca acgtcaacga ctggatcacc gggaccgcgg ccgatggtcc
     1621 gacccctgcg ggcgtcgtgg cgcagacggc gctggcccgc cagcccgaga tgctcagctt
     1681 cctgtccacc cggttcgggc cctacccgtt cgcggacggc ggcgggatcg tcgacgactt
     1741 caccggcctc ggcttcgccc tggagaacca gacccgcccc atctatccgc cggagatctt
     1801 cggcgacccg atcggggccg agagcacgat cgtccacgag ttggcgcacc agtggtacgg
     1861 cgaccgggtc gcggtgtcgg cctggcagca catctggctg aacgagggct tcgccaccta
     1921 cgcggagtgg ctgtgggccc agcgcgaggg cttggccacg gcgcaggaga tcttcgactt
     1981 cttctacagc acccccgcgg gcgacccgct ctggcatcta cgggtcggcg accccggccc
     2041 gaggcatctc ttcgacgctc ccgtctacct ccggggcggc atgaccctgc acgccctgcg
     2101 cctcgaagtc ggcaacgccg acttcttcac catcatgcgc cggtgggccg ccgccccggt
     2161 gggccgcccc accacgaccc acaacttcca agccttggcg gagagggtgt cgggccgaca
     2221 actggacacg ctgttccacg agtggctgtt caccacccac aagcccgcgc tcaccga
//
LOCUS       DASTFV010000608         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2334810, whole genome shotgun sequence.
ACCESSION   DASTFV010000608 DASTFV010000000
VERSION     DASTFV010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2334810"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..747)
                     /locus_tag="VFP02_11130"
     CDS             complement(28..747)
                     /locus_tag="VFP02_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HET9543630.1"
                     /translation="MPDARPRPPVSEHHHRDVQGGVARAAVFGASDGLVSNVSLILGV
                     AGADPGAGVVRLAGLAGLIAGAVSMAAGEYGSMKAQQELLERELAVERRELERNPHVE
                     TVELTQLYQSRGVDPDLARQLAQEVMVDPETALRAHAREELGIDPDALGSPVRAAASS
                     FVSFSLGALLPLLPWFVTSGTTAIVASVVLAVAGAAGLGVALARFTQGSPFRLAVRYV
                     AIAVLAGAVTYGIGSAVGVST"
     gene            770..1804
                     /gene="rlmN"
                     /locus_tag="VFP02_11135"
     CDS             770..1804
                     /gene="rlmN"
                     /locus_tag="VFP02_11135"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET9543631.1"
                     /translation="MPTRYDLDREGLETLLADQPRYRVDQVWEGLYRQGRDLTAITNL
                     PLALRTDLAQRLPLGLAEVARRVGDAGETVKWAWEVDGGHRVETVLLHYAKRSTVCVS
                     TQAGCAMACDFCATGQAGFARHLSAGEIVEQVVMARREAGERRVSNIVFMGMGEPLAN
                     YDATWAAVERLHGGLGIGARGLTVSTVGIVPGILRLAEEVLPVNLAVSLHAANDTLRD
                     ELVPINRRYPLAELEAACALWREVKSRRLSFEWAMIAGTNDRPEDADELAAIAGRLRA
                     HVNLIPLNPTPGFGTQGSSPETVEAFRDQVAATGVPVTIRQNRGVDIDAACGQLAGTP
                     VSAPGRSPAH"
     gene            complement(1773..>2276)
                     /locus_tag="VFP02_11140"
     CDS             complement(1773..>2276)
                     /locus_tag="VFP02_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543632.1"
                     /translation="GTAGAVGALRDWLDGRPAVIVNADGWCVGGLDRLLEGWDGERVR
                     ILLPGGGDFKAQSMIAGSLLPWALAQTIEARPAGLYEAVWRRALAQGTLETVAFPGPF
                     VDCGNPAAYLAANLAASGGQSVVAPGAQVAGRVVRSVCWEDAMVGPDETLVDAIRFSE
                     RGTVLVR"
BASE COUNT          341 a          847 c          825 g          263 t
ORIGIN      
        1 cacgaccgcc ttcgaagcgg ccttcgacta ggtggagacg ccgacggcgg agccgatgcc
       61 gtaggtgacg gcgccggcga ggacggcgat ggccacgtac cggacggcca gacggaacgg
      121 agaaccctgc gtgaagcggg cgagggccac gccgagcccg gcggcaccgg ctacggcgag
      181 gaccaccgag gccacgatgg ccgtggtgcc cgacgtcacg aaccagggca gcaggggcag
      241 gagagcgccg agtgagaagg acacgaacga cgaggccgcg gcccgcaccg gcgagcccag
      301 ggcgtcggga tcgatcccca gctcttccct ggcgtgggcg cggagggccg tctcgggatc
      361 gaccatgacc tcctgggcca gctggcgggc cagatcgggg tcgaccccac gggactggta
      421 gagctgggtc aactccaccg tctcgacgtg ggggttgcgc tcgagctccc ggcgctcgac
      481 cgccagctct cgctcgagca gctcctgctg ggccttcatg gagccgtact cccccgcggc
      541 catcgacacc gcgccggcga tcaggccggc gagcccagcc aggcggacca cgccggcccc
      601 ggggtcggcc ccggccaccc cgaggatcag ggagacattg gagacgaggc cgtcgctggc
      661 cccgaagacc gcggcccggg ccacaccccc ctggacgtcg cgatggtggt gctcgctgac
      721 cggcggacga ggtcgggcat cgggcacggg gcaaccgtac gctcggggcg tgccgacccg
      781 ctatgacctg gaccgcgagg gcctcgagac gctcctcgcc gaccagcccc gctaccgggt
      841 cgaccaggtg tgggaggggc tctaccgcca ggggcgggac ctcacggcga tcaccaacct
      901 gccgctggcc ctgcggaccg atctggccca gcgcctgcct ctcggcctgg ccgaagtggc
      961 ccggcgcgtc ggcgacgcgg gcgagaccgt gaagtgggcc tgggaggtcg acggcggcca
     1021 ccgggtcgag accgtcctgc tccactacgc gaagcggagc acggtctgtg tgagcaccca
     1081 ggcgggctgc gcgatggcct gcgacttctg cgccaccggc caggccgggt tcgcccgcca
     1141 cctcagcgcc ggcgagatcg tcgagcaggt cgtcatggcc cggcgcgagg cgggcgagcg
     1201 acgagtgtcc aacatcgtgt tcatggggat gggcgagcca ctggccaact acgacgccac
     1261 ctgggccgcc gtcgaacgcc tccacggcgg cctcggcatc ggggcccgcg gcctcaccgt
     1321 gtcgacggtc ggcatcgtgc ccggcatcct gcgcctggcc gaggaagtgc tcccggtcaa
     1381 cctcgcggtg tcgttgcacg cggccaacga cacgctccgg gacgagctgg tgcccatcaa
     1441 ccggcggtac ccactggcgg agctggaggc ggcgtgtgcc ctctggcgcg aggtcaagag
     1501 ccggcggctc agcttcgagt gggcgatgat cgccgggacc aacgaccgtc ccgaagacgc
     1561 cgacgagctg gccgccatcg cggggcggct gcgggcccac gtcaacctca tccccctgaa
     1621 cccgacaccg gggttcggga cccagggctc ctccccggag accgtcgagg cgtttcggga
     1681 ccaggtcgcg gccaccgggg tgccggtcac gatccgccag aaccgcgggg tggacatcga
     1741 tgccgcgtgt ggccagctgg ccggcactcc ggtcagcgca ccaggacggt cccccgctca
     1801 ctgaaccgga tggcgtccac gagggtctcg tccgggccga ccatcgcgtc ctcccagcac
     1861 acgctgcgca cgacccgccc cgcgacctgg gcgcccggcg cgaccaccga ctgacctccg
     1921 gacgccgcca ggttggccgc caggtaggcg gccgggttgc cgcagtcgac gaagggcccg
     1981 ggaaaggcga cggtctccag cgtcccctgg gccagggcgc ggcgccagac ggcttcgtag
     2041 aggcccgcgg ggcgggcctc gatcgtctgg gccagagccc acggcaggag cgaccccgcg
     2101 atcatcgact gagccttgaa gtcgcccccg cccggtagca ggatccgcac ccgttcgccg
     2161 tcccaaccct cgagcaggcg gtccaggccc cccacgcacc acccgtcggc attgacgatc
     2221 acggccggtc gcccgtcgag ccagtcgcgg agcgccccga cggcccccgc cgtgcc
//
LOCUS       DASTFV010000609         2524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2354657, whole genome shotgun sequence.
ACCESSION   DASTFV010000609 DASTFV010000000
VERSION     DASTFV010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2354657"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VFP02_11145"
     CDS             <1..280
                     /locus_tag="VFP02_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543633.1"
                     /translation="MICVFLGFVLGEDPVIKMMGIGLATAVFVDATIVRVVLVPATMR
                     LLGDANWWLPAWLDRLLPHIDVEGGNGLPEPEFEDRVLPSAEPVLVDA"
     gene            complement(292..1422)
                     /locus_tag="VFP02_11150"
     CDS             complement(292..1422)
                     /locus_tag="VFP02_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543634.1"
                     /translation="MTVPAGDLRAAVATMMAAHWREGGYTVPHGSVYPWQWLWDSCFH
                     SLIWAELGDGDRAVAELANVLAVQDELGFVPHMTYHGDPAARADFWGRPGTSSITQPP
                     VYGHAAAELVRRGVAIPDELVERAVDGVRFLLEHRARSTEGLVTVVHPWETGCDDSPR
                     WDGWCPGGYERARFREVKGELLASVEWSEGGAPLANPAFAVGSAGFNGLVAWNALELA
                     GVTGDDGLATAAADLTEALDGRWSGDRATWTDDGPHVSGAVRTADGLLPTLLLTRDRD
                     EGFAALGDRTGLGGPFGAAGTDRREPAYDPTSYWRGPVWPQVTYLLWVAASRAGHPVA
                     GELATGLRAGALASGLAEYWHPDTAAAGGAVPQSWTGLALLV"
     gene            1432..2460
                     /locus_tag="VFP02_11155"
     CDS             1432..2460
                     /locus_tag="VFP02_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543635.1"
                     /translation="MSTCAIVSFRLGGTDGVSIVAEAWAASLTELGFTIRTVAGEGPV
                     DRLVPGLAIDVATPPTNGEVAEALADVDLVVVENLATIPLNLPAARVVSAVLAGRPAI
                     LHHHDPPWQRAHFAHITELPPTDPRWRHVCINRRTQRELAWRGIEAVTIYNGFDPDPP
                     PGDREGTRSALGVAPDEPLLVHPVRAIERKNVPAALALAEAVEGTYWLPGGAEEGYGP
                     ILEGLLAGAACRVIHRAWTGRSDLYAAADAVLFPSTWEGFGNPPVEAALHGRQVAVGE
                     YPVAEELRVLGFRWLPADDPAPLAAWLAAPDPAVLAHNRSVAEAHMSLAAQTESLRNL
                     LDQAGWLP"
BASE COUNT          320 a          967 c          904 g          333 t
ORIGIN      
        1 catgatctgt gtcttcctgg gcttcgtgct cggcgaggac ccggtcatca agatgatggg
       61 catcggcctg gctacggccg tgttcgtgga cgccaccatc gtccgggtgg tcctggtgcc
      121 cgcgaccatg cgcctgctcg gtgacgccaa ctggtggctg ccggcctggc tggaccgcct
      181 cctgccccac atcgacgtgg aaggcggcaa cggcctcccc gagcccgagt tcgaggaccg
      241 cgtgctcccg tccgccgagc ccgtcctcgt cgacgcctga gtgggcgccc gtcagaccag
      301 cagggcgagg ccggtccagg actgggggac ggcgccgcct gcggccgccg tgtcggggtg
      361 ccagtactcg gccaacccgg aagccagggc cccggcccgc aacccggtcg cgagctcacc
      421 ggcgacgggg tgacccgccc gtgaggcggc cacccagagc aggtaggtga cctggggcca
      481 gacggggccc ctccaatagg aggtcgggtc gtaggccggc tcccgccgat cggtccccgc
      541 ggcgccgaac gggccgccca ggcccgtccg gtcgcccagc gcggcgaagc cctcgtcccg
      601 gtcgcgggtg agcaggagcg tcggcagcag cccgtctgcc gttcgcaccg cgcccgagac
      661 atgggggccg tcgtcggtcc aggtcgcccg gtcgccggac caccggccgt ccagggcctc
      721 ggtcaggtcg gccgccgccg tggccaggcc gtcgtcgccg gtcacgccgg ccagctcgag
      781 cgcgttccag gccaccagcc cgttgaaacc ggcggagccg acggcgaagg cggggttggc
      841 caggggcgcg ccgccctcgg accactcgac gctggccagc agttcgccct tcacctcccg
      901 gaagcgagcc cgctcgtatc cgcccgggca ccagccgtcc cagcggggac tgtcgtcgca
      961 acccgtctcc cagggatgga cgacggtgac caggccctcg gtcgaccggg ctcggtgttc
     1021 caggaggaag cgcacgccgt cgacggcccg ctcgaccagc tcgtcgggga tcgccacacc
     1081 ccggcgcacc agctcggccg cggcgtggcc gtacaccggg ggctgggtga tggaggaggt
     1141 gccggggcgg ccccagaagt cggcccgggc cgcgggatcg ccgtggtagg tcatgtgggg
     1201 gacgaagccc agctcgtcct ggacggcgag cacgttggcc agctcggcca cggcccggtc
     1261 gccgtccccc agctcggccc agatgaggct gtggaagcag gagtcccaca accactgcca
     1321 ggggtagacc gacccgtggg ggaccgtgta gcccccctcc cgccagtggg ccgccatcat
     1381 cgtggccacg gcggcccgca gatccccggc cggtacggtc atggccccat catgtcgacc
     1441 tgcgccatcg tctcgttccg tctcggtggc accgacggtg tgtcgatcgt ggccgaggcg
     1501 tgggcggcct ccctgaccga gctcggcttc accatccgca cggtggcggg cgagggtccg
     1561 gtcgaccggc tggtgcccgg gctggccatc gacgtggcga cgcccccgac gaacggcgag
     1621 gtggccgagg cgctggctga cgtcgacctc gtcgtggtcg agaacctcgc caccatcccc
     1681 ctcaacctcc ccgccgcccg ggtcgtcagc gcggtgctgg ccgggcggcc ggcgatcctg
     1741 caccaccacg acccgccgtg gcagcgggcc cacttcgccc acatcaccga gctgcccccg
     1801 accgacccca ggtggcgtca cgtctgcatc aaccggcgca cccagaggga gctggcctgg
     1861 cggggcatcg aggccgtgac catctacaac ggcttcgacc ctgaccctcc accgggcgac
     1921 cgtgaaggga ctcggtcggc gctcggcgtc gcccccgacg agcccctcct ggtccacccc
     1981 gtacgggcca tcgagcgcaa gaacgtcccc gccgcgctgg ccctggccga ggccgtcgag
     2041 ggcacctact ggctgcccgg cggggccgag gagggctacg ggcccatcct cgaggggttg
     2101 ctggcggggg cggcctgccg ggtgatccat cgcgcctgga ccgggcgttc ggacctctat
     2161 gccgccgccg acgccgtcct gttcccgtcg acgtgggagg gcttcggcaa cccgccggtc
     2221 gaggccgctc tgcacggtcg gcaggtcgcc gtcggcgaat atccggtggc cgaagagctt
     2281 cgggtcctcg gcttccggtg gcttccggcc gacgacccgg ccccgctcgc cgcctggctg
     2341 gccgcccccg acccggccgt cctggcccac aaccggtcgg tggccgaggc ccacatgtcg
     2401 ctggccgccc agaccgagtc actccggaac ctgctggatc aggcaggctg gctgccgtga
     2461 ccgaccccgt gcgcgagaag cgggcccgca tcgccaagct ggtcggcctc ggcctcgcgg
     2521 ccgg
//
LOCUS       DASTFV010000610         3058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2356609, whole genome shotgun sequence.
ACCESSION   DASTFV010000610 DASTFV010000000
VERSION     DASTFV010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2356609"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..378)
                     /gene="yidD"
                     /locus_tag="VFP02_11160"
     CDS             complement(118..378)
                     /gene="yidD"
                     /locus_tag="VFP02_11160"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET9543636.1"
                     /translation="MSRAARALRALIVGYQHLRTGRPSPCRFFPTCSAYALEAVEVHG
                     ALRGTGLAARRLCRCHPWGAHGIDLVPEPRHSPASSPDQKVV"
     gene            complement(375..698)
                     /gene="rnpA"
                     /locus_tag="VFP02_11165"
     CDS             complement(375..698)
                     /gene="rnpA"
                     /locus_tag="VFP02_11165"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET9543637.1"
                     /translation="MAKLLVVRGRDSFAALARQGRRVREGPLTVVHRSADTAPRVAFA
                     VGRKVGPAVARNRLRRRLRALWREATPPGGDYLIVAAPAAATATHAELARRLSAALER
                     LAVPA"
     gene            complement(732..866)
                     /gene="rpmH"
                     /locus_tag="VFP02_11170"
     CDS             complement(732..866)
                     /gene="rpmH"
                     /locus_tag="VFP02_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET9543638.1"
                     /translation="MKRTYQPNRRRRARKHGFRHRMSSRGGRAILRGRRQRGRARLSA
                     "
     gene            1406..2833
                     /gene="dnaA"
                     /locus_tag="VFP02_11175"
     CDS             1406..2833
                     /gene="dnaA"
                     /locus_tag="VFP02_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET9543639.1"
                     /translation="MTEHAHDLWDRCRGELEQQVSGGVFLMYFEPTEALEVEGSTLVL
                     GVPSVMARDRLDVRYRPLIDDALAEHAPEITDLRFVVVATDPVEADLLDLPHPSRLDD
                     QNADPFDELPLLDELPVPPEGLDGELTVSAGPADVRPEQTFETFVIGSSNQFANAAAL
                     RVAETPARSYNPLTIYGPSGLGKTHLLHAIGNYIKQNYPTFVVRYVRTELFLYQYVDA
                     IRNNTTTEFKRRYREIDVLLVDDIHFLEGKEGLQEEFFHTFNALHEANRQIVLTSDRP
                     PDAIGTLQDRLRSRFVMGLVTDIQPPDLETRLAILRMKAQRTEVDIPEPTLEFIATNV
                     KTNIRELEGALHRLTAYCRLNDLVCTMSLAQDVLAPILGDTGPKPITIDHILATTSAK
                     FGIPVDEIIGPNRRRQLVTARQVAMYAARDLTDLSYPAIAEEFGGRDHTTIIHAVRKV
                     SDLMAERGQLYEQVTDLIDTLRQPA"
BASE COUNT          532 a         1125 c          992 g          409 t
ORIGIN      
        1 cagcgtgagc ggagtgagaa cgagcatcac tgtcagtgtg agcaggatga tcatgccccc
       61 gtacgagggc cagatctcgt agaaccaggc catcaggttc gcgagcggtt cgaacatcta
      121 gaccaccttc tggtcagggg aagaagcggg cgagtggcgg ggctcgggca ccaggtcgat
      181 gccgtgggcc ccccagggat gacagcggca caaacggcgg gcggccagtc ccgtgccccg
      241 gagcgcaccg tggacctcca cggcctccag ggcgtacgcc gaacaggtcg ggaagaagcg
      301 acaggggctg gggcgaccgg tccgcaggtg ttgatagccc acgatcaggg cgcgcagggc
      361 ccgggcggcg cgactcacgc tggcaccgcc aggcgctcga gcgccgccga cagccgccgc
      421 gccagttcgg cgtgggtggc cgtggcggcg gcgggtgcgg ccacgatcag gtagtcccca
      481 cccggtgggg tggcctcccg ccagagcgcc cggaggcgac gacggaggcg gttgcgggcc
      541 acggcggggc ccaccttgcg gccgacggcg aaagccaccc tgggcgcggt gtccgccgac
      601 cggtggacca cggtcagcgg gccctcccgg acccggcggc cctggcgcgc cagtgccgcg
      661 aacgagtctc gtccgcgcac gaccaggagc ttcgccatgt caccgagcga ccgttctgca
      721 ccgcccggga atcaggcgga gaggcgagcg cggccccgct ggcgccggcc ccgcaggatc
      781 gcccggccac cccgggacga catccggtgg cgaaagccgt gcttccgcgc ccgccgacgg
      841 cggttgggct ggtaggtgcg cttcatctgg tcctccaagg gccgcccggg ccgaggttga
      901 tgccgggtgg cgagatcggt tggcgttcgc cccatcggat cacccgagac aacgggaaaa
      961 cacacgggaa tacgccccga ccacctgaac atgccggtgg ccggtcacgt ttgccggtcg
     1021 acccggcaca cgatcgcgcc acgctagacc cgcgcgaccc ccagcggcaa ccagccgggc
     1081 accggacctt gtcggccacg accccggggt cgggccgtct cgactacgcc gtacgcgaat
     1141 tgggcgctga ccaggggaaa cggcctcgtc tcgcagcagc ccaggggcgg tgctaggttc
     1201 ctcgccctgc gtagacgaga agcgcacccg gccggtcccg accccgtcgg agcggacggg
     1261 cctcatcgag aacggcagtt gtggccagga cgattcggtc agtgacgatc gaccctgctc
     1321 gctgttttcc acaggggcgt ccacaagctg tggacgccct ttcgcatgac gtacaaccgg
     1381 gttttggctg gggagctggt ggctggtgac cgaacacgca cacgacctct gggaccggtg
     1441 ccgcggcgag ttggagcaac aggtctccgg tggcgtcttc ctgatgtact tcgagcccac
     1501 cgaggccctc gaggtcgagg gcagcacgtt ggtgctcggc gtgcccagcg tgatggcccg
     1561 tgaccgcctc gacgtgcgct accgcccgct gatcgacgat gccctggccg agcacgcgcc
     1621 cgagatcacc gacctgcgct tcgtcgtcgt cgccaccgac ccggtcgagg ccgacctgct
     1681 cgacctgccc cacccgtcac ggctcgacga ccagaacgcc gatccgttcg acgagctccc
     1741 gctgctcgac gagctgcccg tgccccccga aggcctcgac ggtgagctca cggtgtcggc
     1801 cggccccgcc gacgtccgcc ccgagcagac cttcgagacg ttcgtcatcg gcagctccaa
     1861 ccagttcgcc aacgccgccg cgctgcgggt ggcggagacc ccggcccgct cctacaaccc
     1921 gctgaccatc tacggcccca gtgggctggg caagacccac ctcctccacg ccatcgggaa
     1981 ctacatcaag cagaactacc cgacgttcgt cgtgcgctac gtccgcaccg agctcttcct
     2041 ctaccagtac gtcgacgcca tccggaacaa cacgaccacc gagttcaagc ggcgctaccg
     2101 cgagatcgac gtgctcctcg tcgacgacat ccacttcctc gagggcaagg aggggctcca
     2161 ggaggagttc ttccacacct tcaacgccct ccacgaagcc aaccgccaga tcgtcctgac
     2221 gtccgaccgg ccgccggacg ccatcggcac cctccaagac cgtctccgca gccggttcgt
     2281 catgggcctg gtcaccgaca tccagccgcc cgacctcgag acccggctcg ccatcctgcg
     2341 catgaaggcc caacgcaccg aggtcgacat ccccgagccg accctcgagt tcatcgccac
     2401 gaacgtgaag accaacatcc gcgagctgga gggggccctg caccggctca ccgcctactg
     2461 ccggctcaac gacctcgtgt gcaccatgtc gctggcccag gacgtgctgg ccccgatcct
     2521 cggcgacacc gggccgaagc cgatcaccat cgaccacatc ctggccacca cctcagccaa
     2581 gttcgggatc ccggtggacg agatcatcgg ccccaaccgc cgacgccagc tggtcaccgc
     2641 ccggcaggtc gccatgtacg cggcccggga cctcaccgac ctgagctacc cagccatcgc
     2701 cgaggagttc ggcggacggg atcacaccac gatcatccac gcggtgcgca aggtctcgga
     2761 cctcatggcc gagcggggcc agctctacga acaggtcacg gacctgatcg acaccctccg
     2821 ccagccggcc tgagctgggg acgggccacg ggatgaacgg tggacaacgc cccctccgag
     2881 ccgggagcgc ctggggacaa ggcaacggtt ctccccaggc tcccgcgtgg cgccggccgg
     2941 cccaaggaac gacagggaac agggtgggga cagaaaatat ggtcctgacc agcgcaaacg
     3001 cgtggtcgtc cccaatccac agcacttagt agtttccctg gaacaaagat ctctctcc
//
LOCUS       DASTFV010000611         4628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2359583, whole genome shotgun sequence.
ACCESSION   DASTFV010000611 DASTFV010000000
VERSION     DASTFV010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2359583"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFP02_11180"
     CDS             complement(<1..229)
                     /locus_tag="VFP02_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543640.1"
                     /translation="MRSLRTRAVIGAVTIVGVALLIGSVSMVLLVRSSLTGNVREAAE
                     LRADDLATVLEAGGESAADLAVEDDEDALIQV"
     gene            complement(229..906)
                     /locus_tag="VFP02_11185"
     CDS             complement(229..906)
                     /locus_tag="VFP02_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9543641.1"
                     /translation="MRVLVVEDEVRLADGIRKGLEAEGFAVDVAHDGTDGLWRAREHD
                     YDAIVLDIMLPGVNGYRVCAALRAEENWTPVLMLTAKEGDLDEAEALDTGADDYLTKP
                     FAHVVLVARLRALVRRGRPARPVVLTAGDLRLDPGSRRVWRGEDEIALTTRETSLLEF
                     LLQQAGTVVSKREILDHVWDDDFDGDPNIVEVYVRHLRNKLDRPFDRDSIHTVRGSGY
                     RLDPRGG"
     gene            complement(959..2842)
                     /locus_tag="VFP02_11190"
     CDS             complement(959..2842)
                     /locus_tag="VFP02_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9543642.1"
                     /translation="MRMGPHMALGSDPSAIVGARVQKGLIRRVWRFARAYRPMLAGFL
                     ATIVLAAIIDIVPPFLIRRIIDDAIPQEDRTAVAVLAGLMIVAALAEAALSLGERLWS
                     SKIGEGLIYDLRVALFDHVQRMPLGFFTRTQTGALVSRMNNDVIGAQRAITGTLGAVV
                     SNVVVVATTVVAMLYLEWRLTLISLIVLPLFVVPAKRVGRRLQDLTREQMDLNASMNT
                     TMTERFGVAGAMLVKLFGRQDQEVDEFGERAGRVRDIGVKTAVYSRTFFIALGLVGAV
                     GTALIYWFGSLLVISDALELGTLIALGLLVTRIYTPLTSLTNARVDLMTAFVSFDRVF
                     EVLDTPNPIQDRAGAVDLVDPSGRIELDDVWFTYPTGTEASVASLETPGALPDDADGR
                     PVLKGVSASMDAGTMTALVGPSGAGKSTVASLVPRLYDVTTGALRVDGNDVRDLTQRS
                     LRSAIGVVTQDPHLFHETVRSNLLYARPGATDAELAAACRAARIWDVITALPNGLDTL
                     VGERGYRLSGGEKQRLAIARMLLKDPCIVILDEATSHLDSENEAAVQAALDTALEGRT
                     SVVIAHRLSTIIAADQILVLDEGRIVEQGTHVSLLAEGGLYADLYRTLVRSDEQDVVA
                     PAP"
     gene            complement(2897..4234)
                     /locus_tag="VFP02_11195"
     CDS             complement(2897..4234)
                     /locus_tag="VFP02_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HET9543643.1"
                     /translation="MSETPPSPTVAPAEGSLGILCVGLGAVTTTLIAGVELAKRGEAD
                     PVGSLTQLGTIRIGKRTNGTVPRIQDFVPLASLDQIVWGAWDPYPDDAYTAASRAGVL
                     DQGRHLEGIAETLRAIRPMPAAFDPNYVKRLEGTNVMTDTSKRAMLDQIRADIQAFKD
                     EHLCDRLVMIWAGSTEIFIENGPAHADLDAFEAAIDAEDPTIAPSMLYAYAALQEGVP
                     FANGAPNLAVDTPVLRTFAEEHGVPISGKDFKTGQTLLKTVLAPMLKARMLGLSGWYS
                     TNILGNRDGEVLDDPENFKTKEQSKLGVLEDVLEPERFPELYGDVYHKVRINYYPPRG
                     DNKEGWDNIDLFGWLGYPMQMKVDFLCRDSILAAPLALDLCLFMDLAQRAGLGGIQEW
                     LSFYYKSPQVSPGLYPEHDLFIQLTKLKNTLRWMMDETPLTHLGQDYGEDDLG"
     gene            4307..>4628
                     /locus_tag="VFP02_11200"
     CDS             4307..>4628
                     /locus_tag="VFP02_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9543644.1"
                     /translation="MNDARLVLPNVTVQQLEYLVAAAREPTFARAAAAVGVSPSALSQ
                     GLAELERRVGVTLFAPAGRGRVLAPEAVEVVRYADRVLAETEELARWVVGRRTGRSGP
                     LRVGL"
BASE COUNT          699 a         1511 c         1706 g          712 t
ORIGIN      
        1 tcacctggat gagggcgtcc tcgtcgtcct cgacggccag gtcagcggcc gattccccac
       61 cggcttcgag cacggtcgcc aggtcgtccg cccggagctc ggctgcctcc cgcacgttgc
      121 cggtcaacga cgaccgaacg agcaggacca tgctcaccga gccgatcagg agggcgacgc
      181 ccacgatcgt caccgcgccg atgacggccc gggtgcgcag ggagcgcatc agccgccgcg
      241 cgggtcgagc cggtagcccg agcccctgac ggtgtggatg ctgtcccgat cgaagggccg
      301 gtccagcttg ttgcggaggt ggcggacgta gacctcgacg atgttggggt caccgtcgaa
      361 gtcgtcgtcc cagacgtggt cgaggatctc ccgcttcgac acgaccgtgc cggcctgctg
      421 cagcaggaac tcgagcaggg aggtctcccg cgtggtcagc gcgatctcgt cctccccccg
      481 ccagacgcgg cgcgagccgg ggtcgaggcg gaggtcaccc gcggtgagca cgacgggtcg
      541 ggcgggtcga ccccgccgga ccagcgcccg gagtcgggcc acgagcacca catgggcgaa
      601 cggcttggtc aggtagtcgt cggccccggt gtccagcgct tcggcctcgt cgaggtcacc
      661 ttccttggcc gtgagcatga ggacgggggt ccagttctcc tcggcccgaa gggcggcgca
      721 cacccggtag ccgttgaccc cgggcagcat gatgtccagg acgatggcgt cataatcgtg
      781 ctctcgagcc cgccacaggc cgtcggtccc gtcgtgggcg acgtcgacgg cgaagccttc
      841 ggcctccagc cccttgcgga tgccgtcagc caggcgcacc tcgtcctcga ccaccagcac
      901 ccgcaccgat ccagtctcgc cgaggcacct gaaccctgcc tgaagccgta tccagccctc
      961 agggggcggg ggccaccacg tcctgctcgt cactgcggac caaggtgcgg tagaggtcgg
     1021 cgtagaggcc gccctcggcc aacaggctca cgtgggtgcc ctgctccacg atcctgccct
     1081 cgtccaggac caggatctgg tcggcggcga tgatcgtcga gaggcggtgg gcgatcacca
     1141 ccgaggtccg gccttcgagg gccgtgtcga gggcggcctg gacggcggcc tcgttctcgg
     1201 agtcgagatg gctggtggcc tcgtccagga tgacgatgca ggggtccttc agcagcattc
     1261 gggcaatggc caggcgctgc ttctcgcctc cggagagccg gtagccccgt tcgccgacga
     1321 gggtgtcgag accgttgggg agggcggtga tgacgtccca gatgcgggcc gcccgacagg
     1381 cggctgccag ctcggcgtcg gtggccccag gtcgggcgta gagcaggttg gaccggaccg
     1441 tctcgtggaa gaggtggggg tcctgggtca ccaccccgat ggccgagcgc agcgaccgct
     1501 gggtcaggtc ccggacatcg ttgccgtcga cccgaagggc acccgtggtg acgtcgtaga
     1561 gccgggggac gagcgaggcc accgtggact tgccggcgcc ggacggcccg acgagggcgg
     1621 tcatcgtccc ggcgtccatg gaggccgaca cccccttgag caccgggcgt ccgtcggcgt
     1681 cgtcgggcag ggcccccggg gtctccagtg acgccaccga cgcctcggtg ccggtggggt
     1741 aggtgaacca cacgtcgtcc agttcgattc ggccggaagg gtcgaccagg tcgacggctc
     1801 cggcccggtc ctggatgggg ttgggcgtgt ccagcacctc gaagacccgg tcgaaggaca
     1861 cgaaggcggt catgaggtcg acccgggcgt tggtcaggct ggtcagcggc gtgtagatcc
     1921 gggtcacgag caggcccaga gcgatcagcg tccccagctc cagcgcgtcg gagatcacga
     1981 gcaggctccc gaaccagtag atgagcgcgg tgcccacggc gccgaccagg ccgagggcga
     2041 tgaagaacgt ccggctgtag accgcggtct tcaccccgat gtcccgcacc cggcccgccc
     2101 gctcgccgaa ctcgtcgacc tcctggtcct ggcggccgaa gagcttgacc agcatcgctc
     2161 cggccacccc gaagcgctcg gtcatcgtgg tgttcatcga ggcgttgagg tccatctgct
     2221 cccgggtcag gtcctgcagg cgacggccga cccgcttggc cggtacgacg aagagcggga
     2281 ggacgatcag cgagatgagg gtcaacctcc actcgagata caacatggcc acgacggtcg
     2341 tcgccacgac cacgacgttg gagaccacgg cgcccaacgt cccagtgatc gcccgctggg
     2401 ccccgatcac gtcgttgttc atccggctga ccagggctcc cgtctgggtg cgggtgaaga
     2461 agccgagcgg catgcgctgg acgtggtcga agagcgcgac gcgcaggtcg tagatcagcc
     2521 cctcgccgat cttggaggac cacaaccgct cgcccaggga cagcgccgct tccgcgagcg
     2581 cggccacgat catcagcccg gccagcacgg cgaccgccgt cctgtcctcc tggggaatgg
     2641 catcgtcgat gatccgccgg atcaggaagg gggggacgat gtcgatgatc gcggccaaca
     2701 cgatggtggc caggaaccct gccaacatgg gccggtaggc tcgggcgaac cgccacaccc
     2761 gccggatcag gcccttctgg acgcgggccc cgacgatcgc gctcgggtcg gagccgagcg
     2821 ccatgtgagg tcccatccgc atgcccccga ggctacgggc gccctgtccc gcctcgacag
     2881 ggcccgacgg cggggctcag ccgaggtcgt cctcgccgta gtcctggccg aggtgcgtca
     2941 gaggggtctc gtccatcatc cagcgcaggg tgttcttcag cttggtgagc tggatgaaca
     3001 ggtcgtgctc cggatagagg ccgggagaga cctgcggtga cttgtagtag aagctcagcc
     3061 actcctggat gccgcccaag cccgctcgct gggccaggtc catgaagagg cagaggtcca
     3121 gggccagcgg cgcggccagg atcgagtcgc ggcacaggaa gtcgaccttc atctgcatcg
     3181 ggtagcccag ccacccgaag aggtcgatgt tgtcccagcc ctccttgttg tcgccgcgcg
     3241 gggggtagta gttgatccgg accttgtggt acacgtcgcc gtagagctcg gggaagcgct
     3301 cgggctcgag aacgtcctcc aacacaccga gcttcgactg ctccttggtc ttgaagttct
     3361 ccgggtcgtc gaggacctcg ccgtcccggt tgccgaggat gttggtcgag taccacccgc
     3421 tgaggccgag catgcgggcc ttcagcatgg gggccaggac cgtcttgagg agggtctggc
     3481 ccgtcttgaa gtccttgccg gagatgggga cgccgtgctc ctcggcgaag gtgcgcagga
     3541 ccggggtgtc gacggccagg ttgggggcac cgttggcgaa gggcacgccc tcctggaggg
     3601 cggcgtaggc gtagagcatc gacggcgcaa tcgtcgggtc ctcggcgtcg atggcggcct
     3661 cgaaggcgtc caggtcggcg tgggcagggc cgttctcgat gaagatctcg gtggagccgg
     3721 cccagatcat caccaggcgg tcacagaggt gctcgtcctt gaaggcctgg atgtcggccc
     3781 ggatctggtc gagcatggcc cgcttggagg tgtcggtcat gacgttggtg ccctcgaggc
     3841 gcttcacgta gttggggtcg aaggcggccg gcatcgggcg gatggcgcgg agcgtctcgg
     3901 cgatgccctc gaggtgtcgg ccctgatcga gcaccccggc ccggctggcg gcggtgtagg
     3961 catcgtcggg atacgggtcc cacgcgcccc acacgatctg gtcgagggag gccaggggga
     4021 cgaagtcctg gatacggggg acggtgccgt tggtgcgctt gccgatgcgg atggtgccga
     4081 gctgggtgag ggagccgacg ggatcggctt cgccccgctt ggccagctcc acgccggcga
     4141 tgagcgtggt ggtgacggcg ccgaggccga cacagaggat gccgaggctg ccctcggcgg
     4201 gggcgacggt cggcgaaggc ggtgtctcgg acatggggtg gaccctaagc gataacggat
     4261 gattgggtct tccgatgaag gaagactgaa cagttagagt gtccgggtga acgatgcgcg
     4321 gctggtcctg cccaacgtga cggtgcagca gctggagtac ctggtggccg cggcccggga
     4381 gccgacgttc gcccgggcgg ccgcggcggt cggcgtgtca ccgtcggctc tgtcccaggg
     4441 cctggccgag ctcgagcgcc gggtcggggt cacgctgttc gcgccagccg gccgagggcg
     4501 cgtcctcgct cccgaggccg tcgaggtcgt gcgctacgcc gatcgggtgc tggccgagac
     4561 cgaggagctg gcccgctggg tggtgggccg ccggacgggg cggtccggtc cgctgcgggt
     4621 gggcctca
//
LOCUS       DASTFV010000612         3307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2359920, whole genome shotgun sequence.
ACCESSION   DASTFV010000612 DASTFV010000000
VERSION     DASTFV010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2359920"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1482)
                     /gene="hisD"
                     /locus_tag="VFP02_11205"
     CDS             complement(175..1482)
                     /gene="hisD"
                     /locus_tag="VFP02_11205"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HET9543645.1"
                     /translation="MAPVLAPLLTRLDRRGAADPSADLPRPALGGDEPVEAVRAILAD
                     VAAGGDAAVRAQTERLDGVVLDDLRVPPATVTAALDAVDPDVRSALQQAAEEIAAYHA
                     DQAPPTRRHDRDGVVIDEYYVPVARAGCYVPGGRARYPSTVLMTVVPARAAGVDDVVL
                     CTPPGPDGQVPVETLAAAAIAGADEVYRIGGAQAIGALAHGTESLRPVDVIVGPGNVY
                     VSQAKRQVTGLVGVPAAFAGPSEVVVIGDDTVPAELCATDIVVQAEHGPGGLAWLVTW
                     SEPVADEVDAAITTIVAGAERRADIEATLADGGYTVLVDGPEQAVAVANAIAPEHLQL
                     MTADPAALVPLVRNAGAVFCGPWSPASLGDYAAGPSHVLPTFGSARYAGALSVLDFLK
                     QIHVVTADETGLARLGPVVETLATAEGLPVHAASIAARRTSRP"
     gene            1544..1738
                     /locus_tag="VFP02_11210"
     CDS             1544..1738
                     /locus_tag="VFP02_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543646.1"
                     /translation="MTAHHAQLASTASSLDELIQRVTATADDLAGAQEDLAADLYEVE
                     RSLRTGARRLATVLRRLDRP"
     gene            complement(1892..2533)
                     /gene="nth"
                     /locus_tag="VFP02_11215"
     CDS             complement(1892..2533)
                     /gene="nth"
                     /locus_tag="VFP02_11215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET9543647.1"
                     /translation="MARPRTPAGRARETMRRLIDEYPDARCELDHENPFQLLAATILS
                     AQCTDARVNMVTPHLFARYPTAFDLAEAEPLELEEIIRSTGFFRSKAKSLIGMAQGLV
                     EVHDGEVPSRMADLTQLPGVGRKTANVVRSVALDLPGLPVDTHVGRLSRRLGLTDEED
                     PVKVEAVLNAMIPAAERGRFSLRLILHGRRVCPARRPRCGDCVLNDFCPSSEV"
     gene            2553..3233
                     /gene="mshD"
                     /locus_tag="VFP02_11220"
     CDS             2553..3233
                     /gene="mshD"
                     /locus_tag="VFP02_11220"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: protein motif:HMM:TIGR03448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HET9543648.1"
                     /translation="MATVELDVALGPDEDPAWVAANRLGPAIEAARAIGAPAVRWWVE
                     ATSDRHVEAAERAGLAPVRRLHQLRRPLPLEETTDLVTRPFRPGVDDDAWLAVNNRAF
                     AWHPDQGGWTKETLQGRLAEPWFDADGFLLHEADDRLAGFCWTKVHDGEDPPLGEIYV
                     IAVDPDFGGRGLGRALTLAGLRYLADRGLTVGTLYVEADNAPALALYESLGFTRHHTT
                     TAFEAAFD"
BASE COUNT          450 a         1177 c         1261 g          419 t
ORIGIN      
        1 cggggtagcg gttccaggcc acgtcggcca gcgccgcagc cagctcgtcg acccaggcgg
       61 tcggcgggcc gaccggcgat tcgttggtgt tcagccgcac cgggacgtcg agttggggtg
      121 agtggtaacc ggtcatgagg gccaggtcgc cacggggttg gacccgggtg gtggtcatgg
      181 ccgcgacgtc cgacgggccg cgatcgacgc ggcgtgcacc ggaaggccct cggcggtggc
      241 gagtgtctcg accacgggcc cgaggcgggc caggccggtc tcgtcggcgg tgaccacgtg
      301 gatctgcttg aggaagtcca ggaccgagag ggcgccggca tagcgggctg agccgaaggt
      361 gggcaggacg tggctgggtc cggccgcgta gtcacccagg gaggccggcg accaggggcc
      421 gcagaacacg gccccggcat tgcgcaccag gggcaccagg gccgcggggt cggcggtcat
      481 caactggagg tgctcagggg cgatggcgtt ggccacggcc accgcctgct ccggcccgtc
      541 gaccaacacc gtgtagccgc cgtcggccaa cgtggcttcg atgtcggcgc ggcgttccgc
      601 tccggcgacg atggtggtga tggcggcatc cacctcgtcg gcgacgggct cggaccaggt
      661 gacgagccag gccaggccac ccgggccgtg ctcggcctgg acgacgatgt cggtggcgca
      721 gagctcggcg ggcacggtgt cgtcgccgat gaccaccacc tcggaggggc ccgcgaacgc
      781 cgcgggcaca ccgaccaggc cggtgacctg gcgcttggcc tgggagacgt agacgttgcc
      841 tggtccgacg atgacgtcga ccgggcgaag gctctcggtg ccgtgggcga gggcaccgat
      901 ggcctgggcc ccaccgatgc ggtagacctc gtcggcaccg gcaatggcgg ccgcggccag
      961 ggtctccacc gggacctggc cgtcgggccc gggtggggtg cagagcacga cgtcgtccac
     1021 cccggccgcc cgggccggga ccacggtcat caggacggtc gaggggtagc gggcccgtcc
     1081 gcccggcacg tagcagccgg cccgggccac gggaacgtag tactcgtcga tgacgacccc
     1141 gtcacgatca tggcgccggg tgggcggcgc ctggtcggcg tggtaggccg cgatctcctc
     1201 ggcggcctgc tggagggcgg accgcacatc ggggtccacc gcgtccagtg ccgcggtcac
     1261 cgtcgcgggc gggacccgca gatcgtcgag aaccacgccg tcgaggcgct cggtctgggc
     1321 ccgcacagcg gcgtcacccc ccgccgcgac atcggccagg atcgcccgca cggcctccac
     1381 cggctcgtcg ccgccgagcg cgggccgggg caggtcggcc gacgggtcgg cggcgccccg
     1441 ccggtcgagg cgggtcagca ggggggcgag gacgggcgcc atcggcgcct cacggtaccg
     1501 gcgccccccg ctcgcttcgg tcggtttgcg ctacacaagg ccggtgaccg cccaccacgc
     1561 ccagctggcc tcgaccgcgt ccagcctcga cgagctgatc caacgggtca ccgccaccgc
     1621 cgacgatctg gcgggggccc aggaggatct ggccgccgac ctctacgagg tggagcggtc
     1681 gctgcgcacc ggggcccgcc ggctggcgac ggtgctgcgc cggctcgacc ggccctgacg
     1741 cgagcccggt cccccgatcg ggtgaggccg agcccggacg cggggccggc gcccacgaag
     1801 ggcgccggcc gtgggcgacc tcgggcggcg gatcccgatg gtcgccagta gccaggtggc
     1861 gggaacctgg cggcgccacc ttatcccgaa gtcagacctc agatgatgga cagaagtcgt
     1921 tcaggacgca gtccccgcac cggggacggc gcgccgggca gacccggcgg ccgtggagga
     1981 tgaggcgcag gctgaaccgg ccccgctcgg cggcggggat catggcgttc agcaccgcct
     2041 ccaccttcac cgggtcctct tcgtcggtca gccccaaacg gcgggacaat cggcccacgt
     2101 gcgtgtcgac gggcagcccg ggcaggtcca gcgccacgga tcggaccacg ttcgccgtct
     2161 tgcgaccgac gccgggcagc tgggtcaggt cggccatccg cgacggcacc tcaccgtcgt
     2221 ggacctcgac cagtccctgg gccatgccga tcaggctctt ggccttcgag cggaagaatc
     2281 cggtggagcg gatgatctct tccagctcga ggggctcggc ctcggccagg tcgaaggccg
     2341 tcggatagcg ggcgaacagg tgcggggtga ccatgttcac ccgggcgtcg gtgcactggg
     2401 cggacaggat cgtcgccgcc aggagctgga acgggttctc gtggtccagc tcacagcggg
     2461 cgtccgggta ctcgtcgatg aggcggcgca tggtctcccg agcgcgaccg gcaggcgttc
     2521 gggggcgggc cacgtcggta gtgtgccgcg ccgtggcgac cgtggagctc gacgtcgcgc
     2581 tcggtcccga cgaggatccg gcgtgggtcg cggccaaccg actggggccg gcgatcgagg
     2641 cggcccgggc catcggcgcc cccgccgtgc gctggtgggt cgaggccacc tccgaccgtc
     2701 acgtcgaggc ggcggagcgg gccgggctgg cgccggtccg tcgcctccac cagctgcggc
     2761 gcccgctccc tctcgaggag acgaccgatc tcgtcacccg gccgttccgg cccggagtcg
     2821 acgacgacgc ctggttggcg gtgaacaacc gggcattcgc ctggcacccc gaccaggggg
     2881 ggtggacgaa ggagaccctc cagggccgcc tggccgagcc gtggttcgac gcggacggtt
     2941 tcctgctgca cgaggcggac gaccgcctcg ccgggttctg ctggaccaag gtccatgacg
     3001 gcgaggaccc gcccctgggc gagatctacg tgatcgccgt cgacccggac ttcggcggac
     3061 gcggcctcgg tcgcgccctc accctcgccg ggctccgcta cctggccgat cggggcctga
     3121 ccgtcggcac cctctacgtc gaagccgaca acgcccccgc cctcgcgctc tacgagtccc
     3181 tcggcttcac ccgccaccac accacgaccg ccttcgaagc ggccttcgac taggtggaga
     3241 cgccgacggc ggagccgatg ccgtaggtga cggcgccggc gaggacggcg atggccacgt
     3301 accggac
//
LOCUS       DASTFV010000613         2860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2361173, whole genome shotgun sequence.
ACCESSION   DASTFV010000613 DASTFV010000000
VERSION     DASTFV010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2361173"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFP02_11225"
     CDS             <1..228
                     /locus_tag="VFP02_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543649.1"
                     /translation="ERLRMVGLLAGGVFLATWGVFRLAVLTNPVLPTDVPFAFERLVT
                     ALALGVGVGAVIVAFRSGALTRSLLPDLAEG"
     gene            256..1365
                     /locus_tag="VFP02_11230"
     CDS             256..1365
                     /locus_tag="VFP02_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543650.1"
                     /translation="MVSFAAQLLAFAVGASVVLWVVRAAVWTVVVPRPEQVWLTRVLF
                     VVVRRPFEFFACRARTVTRREAIRARYGPTTLMLMPLAWVTGVMIGFVPIYWALGADP
                     IGEEFVLSGSSVTTLGFSRPNEGPPLGAAVVEAVIGLGLVALLISFLPTMYGHFSRRE
                     HLVAKLSSRAGTPPTPAEMFIRHHRLERLEQLDDMWPDWEDWFVELEESHTTYPALVH
                     FRSIDDHRSWLTGAGAVLDAAALRSSTLDIPRQARAELTIRSGYLALRAIADFFGIHH
                     ERRPQPTDPISIHKAEFDGVYEQLRAAGVPLKPDRDQCWRDFAGWRVNYDRVLLELCA
                     LVQPPPALWSSDRMSDLPRLRFRRPRRPTSVEQEE"
     gene            1367..2548
                     /locus_tag="VFP02_11235"
     CDS             1367..2548
                     /locus_tag="VFP02_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9543651.1"
                     /translation="MSFEVPEHVRPVRAAVEAFMRERVEPAEPELHAGDEAARDLLRE
                     LQAEAKAEGLWALGHPAELGGGGLPFLDYVYVNEVQGRSEFGQLALGTFTLQDSLMLD
                     KYASPEWRERYLAPLVAAEISPSFAMTEPEVASSDPTQLQTAAVLDGEEWVINGLKWF
                     TTGANRAAYTTVMCRTEPDAPDHRAFSMIVVPTDTPGYEIVRETPVLGIKGGHCEVRY
                     TDVRVPAGNLLGPRGHGFQIAQDRLGPGRIFHCMRWLGQAQRAFELLCERLNARVAFG
                     EPLSHKQLMQQHVFESYAEIQACRLLTLQAAERIDAGDQARVEIGTIKVVGARMLHNV
                     IDRAIQAHGAKGLTDDTPLSFMYRSARFARIYDGPDEVHVHTVARRILKQSRDGNPIT
                     F"
     gene            complement(2611..>2860)
                     /locus_tag="VFP02_11240"
     CDS             complement(2611..>2860)
                     /locus_tag="VFP02_11240"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET9543652.1"
                     /translation="LFAVAEAYPDLKADAHFLELQKELAATENRIQAARRIFNGNVRD
                     LNRRVQQVPSNLVAKLGGFHRAEYFQIDAAAREVPSVV"
BASE COUNT          386 a         1034 c          996 g          444 t
ORIGIN      
        1 gagcgcctgc gcatggtcgg ccttctcgcc ggcggggtgt tcctggccac ctggggcgtg
       61 ttccggctcg cggtgctgac gaacccggtg ctcccgacag acgtcccgtt cgccttcgag
      121 cgcctggtca ccgcgctggc cctcggtgtc ggggtcggcg ccgtgatcgt cgccttccgc
      181 tccggcgccc tcacccgctc cctcctgcct gacctcgccg aggggtagca gcctcgccgt
      241 tacgctccgg cgcgcatggt gagtttcgcg gcgcaactcc tggcgttcgc cgtcggcgcg
      301 tcggtcgtgc tctgggtggt gcgggccgcg gtgtggacgg tggtggtgcc gcggcccgag
      361 caggtgtggc tcacccgggt cctcttcgtg gtggtccgcc ggccgttcga gttcttcgcc
      421 tgccgcgcgc gcaccgtgac ccggcgggag gcgatccggg cccgctacgg ccccacgacc
      481 ctgatgctga tgcccttggc gtgggtgacc ggggtgatga tcgggttcgt gcccatctac
      541 tgggccctcg gcgccgaccc catcggcgag gagttcgtgc tgtccggttc gtcggtcacc
      601 acgctgggct tctcccgccc caacgagggc ccgccgctgg gcgccgcggt ggtcgaggcg
      661 gtcatcggcc tgggcctggt cgccctgctc atcagcttcc tgcccacgat gtacggccac
      721 ttctcccgcc gggagcacct ggtggccaag ctctccagca gggcgggcac cccgccgacg
      781 cccgccgaga tgttcatccg gcaccaccgc ctcgagcggc tcgagcagct cgacgacatg
      841 tggccggact gggaggactg gttcgtcgag ctggaggaga gccacaccac ctatccggcg
      901 ctggtgcact tccgctccat cgacgaccac cgctcctggc tcaccggggc gggggcggtg
      961 ctcgacgcgg ccgccctgcg gtcctccacc ctcgacatcc cgcggcaggc gagagccgag
     1021 ctgacgatcc ggtcgggcta tctcgccctg cgggccatcg ccgacttctt cgggatccac
     1081 cacgagcggc gaccccaacc cacggatccg atctcgatcc acaaggccga gttcgacggc
     1141 gtctacgagc agctccgggc cgccggggtc ccgctgaagc cggaccggga ccagtgttgg
     1201 cgggacttcg ccggctggcg ggtcaactac gaccgggtgc tgctcgagct gtgtgcgctg
     1261 gtgcagccgc cccccgccct gtggtcctcg gaccgcatga gcgacctgcc ccggctcagg
     1321 ttccgtcgtc cgcggcgacc cacgtccgtc gaacaggagg agtgagatga gcttcgaggt
     1381 gcccgaacat gtgcggccgg tgcgagcggc cgtggaggcg ttcatgcggg agcgggtcga
     1441 gcccgccgaa ccggagctcc acgccggcga cgaggccgcc cgcgacctcc tgcgggaact
     1501 gcaggccgag gccaaggccg agggcctgtg ggccctcggg cacccggccg agctgggcgg
     1561 cgggggcctg cccttcctgg actacgtcta cgtcaacgag gtccagggcc ggagcgagtt
     1621 cggccagctc gccctcggca ccttcaccct ccaggactcc ctgatgctgg acaagtacgc
     1681 cagccctgag tggcgggagc gctacctcgc ccccctggtg gcggccgaga tctcgcccag
     1741 cttcgccatg acggagcccg aggtggcgtc gtcggatccc acccagctcc agaccgccgc
     1801 cgtgctcgac ggcgaggagt gggtcatcaa cggcctgaag tggttcacga cgggggccaa
     1861 ccgggcggcc tacacgacgg tcatgtgccg gaccgagccc gacgccccgg accaccgcgc
     1921 cttctcgatg atcgtggtcc cgaccgacac gcctggctac gagatcgtca gggagacgcc
     1981 ggtgttgggc atcaagggtg ggcactgcga ggtccgctac accgacgtgc gggtgccggc
     2041 cggcaacctc ctcggccccc gggggcacgg cttccagatc gcccaggacc ggctgggccc
     2101 cgggcggatc ttccactgca tgcggtggct ggggcaggcc cagcgggcct tcgagctgct
     2161 ctgcgagcgg ctgaacgcgc gggtggcctt cggcgaaccg ctgtcgcaca agcagctcat
     2221 gcagcagcac gtgttcgagt cctacgccga gatccaggcc tgccgcctgc tcaccctgca
     2281 ggccgcggag cgcatcgacg ccggcgacca ggcccgggtg gagatcggca ccatcaaggt
     2341 ggtcggggcc cggatgctgc acaacgtcat cgaccgggcg atccaggccc acggggccaa
     2401 gggcctgacc gacgacacgc cgctcagctt catgtaccgc tcggcccgct tcgcccggat
     2461 ctacgacggc cccgacgagg tccacgtcca caccgtcgcc agacgcatcc tcaaacagtc
     2521 ccgcgacggg aacccgatta ctttctgatc cacccagcgg gtcgccggcg acacagaagc
     2581 gtccccagaa ccgttaggtg tgcctgacag tcagacgacc gatgggacct cgcgggcggc
     2641 tgcgtcgatc tggaagtact cggcccggtg gaagccaccg agcttggcga caaggttcga
     2701 cggcacctgc tgaacgcgcc ggttcaggtc ccggacgttg ccgttgaaga tgcgccgggc
     2761 ggcctggatg cggttttcgg tggcggccag ctccttctgg agctccagga agtgggcgtc
     2821 ggccttcagg tcggggtagg cctcggccac ggcgaacagc
//
LOCUS       DASTFV010000614         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2364143, whole genome shotgun sequence.
ACCESSION   DASTFV010000614 DASTFV010000000
VERSION     DASTFV010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2364143"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VFP02_11245"
     CDS             complement(<1..1067)
                     /locus_tag="VFP02_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9543653.1"
                     /translation="MLRMGALLLAFGLIAAACGGSDDSGGDDASGENPRGEESSDPDD
                     GAEPQRGGSIIFAREAETSSPWTPSAMICDVACHQAIKGIYDTLTWPDADGEVHGMLL
                     ESIEPNADFTEWTLTPRDGIQFHDGTPWDVAALDTHFSTMRDSVLVGNVFNDITDQQV
                     QGNTLVLTMRNPWTHFPLFLSGQPGYVASPTWLAAVADGSASEAEPVGTGPFEFEAYS
                     PGDSFSMTRNENYWLMAPDGEPYPYLDEIEFLVQDENQTRDNAIISGDIDITHMDSGD
                     SIERLRQEADNGTINLFEIDQRQETGYVLINSSDPNAAVSDVRIRQAMAYAFDHVVEN
                     QSRNAGIFEIANGPFSPGSIG"
     gene            complement(1210..1869)
                     /locus_tag="VFP02_11250"
     CDS             complement(1210..1869)
                     /locus_tag="VFP02_11250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET9543654.1"
                     /translation="MAPKSSWLPVDLHQYVVDHGRPPDPLLVELAAETEATLGATARM
                     QISPEQGAFLEWLTRLLGATNVIEVGTFTGYSSICLARGLAAGGHLLCCDVSEEWTAV
                     ARAYWERAGLTDRIELRLAPAAETLAAVPHDPPVDLAFVDADKEGYPVYLDLLAERLR
                     PGGVIAVDNTLWGGAVADPSVTDPTTQLLRDLNDGLVDDARFDTVLLPLGDGLTLLRK
                     R"
BASE COUNT          304 a          665 c          777 g          329 t
ORIGIN      
        1 tagccgatgg agccggggga gaaggggccg ttggcgatct cgaagatgcc ggcgttgcgg
       61 gactggttct ccaccacgtg gtcgaaggcg taggccatgg cctggcggat ccggacgtcg
      121 ctgacggcgg cgttcggatc cgaggagttg atcaggacgt agcccgtctc ctggcgctgg
      181 tcgatctcga agaggttgat cgtgccgttg tcggcctcct ggcggaggcg ctcgatcgag
      241 tcccccgagt ccatgtgggt gatgtcgatg tcgccggaga tgatggcgtt gtcccgggtc
      301 tggttctcgt cctgcaccag gaactcgatc tcgtccaggt acgggtaggg ctcgccgtcg
      361 ggggccatca gccagtagtt ctcgttgcgg gtcatcgaga agctgtcgcc gggcgagtac
      421 gcctcgaact cgaacgggcc ggtaccgacg ggctcggctt cggaggcgct gccgtcggcc
      481 acggccgcca gccaggtcgg cgaagccacg taaccgggct gaccgctgag gaagagcggg
      541 aagtgcgtcc acgggttgcg catcgtcagg accagtgtgt tgccctggac ctgctggtcc
      601 gtgatgtcgt tgaacacgtt gccgaccagg accgagtccc gcatcgtcga gaagtgggtg
      661 tcgagcgcag ccacgtccca gggggtgccg tcgtggaact ggatgccgtc acggggcgtg
      721 agggtccact cggtgaagtc ggcgttgggc tcgatggact cgagcagcat cccgtggacc
      781 tcgccgtcgg cgtcgggcca ggtcagcgtg tcgtagatgc ccttgatggc ctggtggcag
      841 gccacgtcgc agatcatcgc cgagggcgtc caggggctgg acgtctccgc ctcgcgtgcg
      901 aagatgatgg agccgccgcg ctgcggctcg gccccgtcgt cggggtcgct ggactcctcg
      961 ccccgggggt tctcgccact ggcgtcgtcg ccaccggagt catcgctgcc tccgcaggcc
     1021 gcggcgatca gcccgaaggc caggagcagg gctcccatcc gcagcatcca gcgtcgtgcc
     1081 gaccagctcg tcatgtgtgc tcccccgcgt cctcgaatgg tgctgtcacg caccggagga
     1141 cgacctccca gcttgtgaca gcggacacag tagaccgcgc cactcgccgc gcgcgtacgc
     1201 ccctcggctt cagcgcttgc gcaggagggt gagcccgtca cccaggggga gcagcaccgt
     1261 gtcgaagcgg gcgtcgtcga ccaggccgtc gttcaggtcc cggaggagct gggtggtcgg
     1321 gtcggtcacg ctgggatcgg cgaccgcgcc gccccacagc gtgttgtcga cggcgatgac
     1381 cccgcccgga cgcagacgct cggccaggag gtcgaggtag acgggatacc cctccttgtc
     1441 ggcgtcgacg aaggcgaggt cgaccggcgg gtcgtggggg acggcggcca gggtctccgc
     1501 ggccggggcg aggcgcagct cgatgcggtc ggtcaggccg gcccgttccc agtaggcgcg
     1561 ggcgacggcg gtccactcct cgctgacgtc gcagcagagg aggtggccgc ccgccgccag
     1621 cccgcgggcg aggcagatcg aggagtaccc ggtgaacgtc ccgacctcga tcacgttcgt
     1681 cgcaccgagc aggcgggtca gccactcgag gaaggcaccc tgctccggcg agatctgcat
     1741 ccgtgcggtg gcgccgagcg tcgcctcggt ctccgccgcc agctcgacga gcagcgggtc
     1801 cggtggccgg ccgtggtcaa cgacgtactg gtgcaggtcg acgggcaacc acgacgattt
     1861 gggggccatg accccatgat gccggagtcg tcgtcggtgc ggcccgccgg gggcggggca
     1921 atgtggacaa ccgtggggac aacgggtgga tttcggtgaa ctccctgggg atctcgcgaa
     1981 aacccttgac caaccttcgg gtggcacgcg aaggtgcgcc caagccagtc agggcggccg
     2041 gagcggccgg aggcaccgga ggttcgtcca ccgga
//
LOCUS       DASTFV010000615         2903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2365094, whole genome shotgun sequence.
ACCESSION   DASTFV010000615 DASTFV010000000
VERSION     DASTFV010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2365094"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFP02_11255"
     CDS             complement(<1..430)
                     /locus_tag="VFP02_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543655.1"
                     /translation="MNRGERGAVISGVGQSQIGRRIYRDPLDLTIESTFAALENAGLT
                     VADIDGISTYPGALSTPPGFSGVGVTELQEALHLELNWFSGGIESPGQFGSVINAIAA
                     VAMGLCRHIVCFRTVWEASAQGDQGRASVTLGGGGGGGYRA"
     gene            487..756
                     /locus_tag="VFP02_11260"
     CDS             487..756
                     /locus_tag="VFP02_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543656.1"
                     /translation="MAATATATRPRRATGAKKQSWKSRAVYRLIEAGSAVTCVNCEEH
                     IKFKAKLRAKQVICNVYSKGVWQRVEHFHDECYTESGQPYGPAGD"
     gene            complement(735..1394)
                     /locus_tag="VFP02_11265"
     CDS             complement(735..1394)
                     /locus_tag="VFP02_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543657.1"
                     /translation="MAVAVLLGACAGGDDGGGSDDTAPASTSTSVPSAATARLNQLLL
                     RTRNLGDGDYRAVVVSRPFSAPRASRIRLCDQDIRAQLRTVAGRQSRFSNGTVEVSHT
                     VTSGGDTTDFLQRFESVVAGCPGPWREAPLPTGGGPVTREIIGTYPVPDVGVDGAGAI
                     IRSSNAAGSTDTVVIVLVQGAVVSSLSVSGPEGADFDVVEPAIRGAADRLLAAQSPAG
                     P"
     gene            complement(1415..2029)
                     /gene="cysC"
                     /locus_tag="VFP02_11270"
     CDS             complement(1415..2029)
                     /gene="cysC"
                     /locus_tag="VFP02_11270"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HET9543658.1"
                     /translation="MTRSENVRWHESERPRDERWGAVGLHGVTVWFTGLSGSGKSTVA
                     VAVEDRLLDAGRPTYLLDGDNLRHGLNGDLGFSAEDRHENVRRVGEVARLFADAGVVA
                     LVPIISPYRDDRDRARAAHDAAGLLFVEVFVDTPIEECERRDPKGLYAQARAGEIKGF
                     TGIDDPYEAPVAAELVLTPADGGAGAQAERVLALLADRGVLPPR"
     gene            2121..2873
                     /locus_tag="VFP02_11275"
     CDS             2121..2873
                     /locus_tag="VFP02_11275"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HET9543659.1"
                     /translation="MPADPRDHVEAARIADAAGELLVALRARLSDEGVDAATLKDEGD
                     RRANELILTLLAEAYPDDAVLSEEAKDDKARLEADRIWIVDPLDGTREYSEPPRVDWA
                     VHIALVDRAGTPLAGAVALPGRALVLGTDPPVVPAERQAGPPRVVISRTRPPAEALAV
                     AEALSGEVVEMGSAGAKAMAVVLGEVDVYVHSGGQYEWDSCAPVAVARAAGLHTSRMD
                     GSPLVYNQDDVYLPDLLICRTEYVQPVLDLLN"
BASE COUNT          431 a         1077 c         1012 g          383 t
ORIGIN      
        1 cggcgcggta gccgccaccc ccgcccccac ccaaggtcac ggaggcccgc ccctggtcgc
       61 cctgggctga cgcctcccac acggtgcgga agcagacgat gtgccggcag aggcccatgg
      121 cgacggcggc gatggcgttg atcaccgagc cgaactgccc cggcgactcg atgcccccgg
      181 agaaccagtt cagctcgagg tgaagcgctt cctgcaactc cgtgacccct acgcccgaga
      241 acccgggcgg ggtgctcagc gctccggggt aggtcgagat gccgtcgatg tccgcgaccg
      301 tcaggccggc gttctcgagg gcggcgaagg tgctctcgat ggtcagatcc agcgggtccc
      361 ggtagatgcg gcggccgatc tggctctggc ccaccccgct gatgaccgct ccccgctcgc
      421 cccggttcat caccctctct ctgtaccaga cctaacagcc ggtgcggcgc ggccgacggg
      481 tacggcatgg cagcgaccgc aaccgcgact cgcccccgtc gggcgacggg tgcgaagaag
      541 cagtcctgga agagccgcgc cgtctaccgc ctcatcgagg caggcagcgc cgtcacctgt
      601 gtgaactgcg aggagcacat caagttcaag gccaagctcc gggccaagca ggtgatctgc
      661 aacgtctaca gcaagggcgt ctggcaacgc gtagagcact tccacgacga gtgctacacc
      721 gagtcgggtc agccctacgg ccccgccggg gactgagccg ccagcagccg gtcggccgcc
      781 ccccgaatgg cgggttcgac cacgtcgaag tcggcgccct cgggacccga gacgctgagg
      841 ctcgacacca ccgccccctg gacgaggacg atcacaaccg tgtccgtgct cccggcggca
      901 ttgctggacc gaatgatcgc tccggcgccg tccaccccga catcgggcac cgggtaggtc
      961 ccgatgatct cccgggtgac cggtccccct cccgtcggca gcggtgcctc cctccagggg
     1021 ccggggcacc cggccaccac cgactcgaag cgctggagga agtcggtggt gtcgccgccg
     1081 ctggtgacgg tgtggctgac ctcgaccgtg ccgttcgaga atcgggactg acgtccggcg
     1141 accgtgcgca gctgggcccg gatgtcctgg tcgcagagtc ggatgcggga tgcccgcggg
     1201 gccgagaacg gccgggacac gacgacggcg cggtagtccc cgtcgcccag gttccgcgtc
     1261 cggagcagga gctggttcaa ccgagccgtc gccgcgctcg ggactgaggt ggaggtggac
     1321 gccggcgccg tgtcgtcgct cccaccgccg tcgtcgccac cggcacaggc acccagcagc
     1381 accgcgacgg ccaccgcgac tgcgactcgt cgcctcaccg gggagggagc acgccccggt
     1441 cggcgagcag agccaggacc cgctcggctt gggctccggc gccaccgtcg gccggggtga
     1501 ggacgagctc agccgccacc ggggcctcgt aggggtcgtc gatcccggtg aaccccttga
     1561 tctcccccgc ccgggcctgg gcgtagagac ccttgggatc ccgccgctca cactcctcga
     1621 tcggggtgtc gacgaagact tcgacgaaga gcaggccggc ggcgtcatgg gcggcgcggg
     1681 cgcggtcccg gtcgtcgcgg tacgggctga tgatggggac cagcgccacc acgccggcat
     1741 cggcgaacag ccgggccacc tcacccaccc gccggacgtt ctcgtgacga tcctcggcac
     1801 tgaagcccag gtcgccgttc aggccgtgac ggaggttgtc gccgtcgagc aggtaggtcg
     1861 gtcgtccggc gtccaggagc cggtcctcga cggcgacggc cacggtcgac ttgcccgagc
     1921 cggagaggcc cgtgaaccag acggtcacgc cgtgcaggcc gacggcaccc caccgttcgt
     1981 cacggggccg ctcactctcg tgccatcgca cgttctcgct gcgcgtcatg cggtcgggag
     2041 ggtacggccc cgcccccgcc gacggcgaac gccccaccga accgggaggc gccggcacgg
     2101 ccgggctagc gtcgtccgcc gtgcctgcgg acccccgtga ccatgtcgaa gccgctcgga
     2161 tcgccgacgc agccggcgag ctgctcgtgg cgctgcgggc tcgcctgagc gacgaaggcg
     2221 tcgacgccgc caccctgaaa gatgagggcg accggcgggc caacgaactg atcctgaccc
     2281 tgctggccga ggcctatccc gacgacgccg tcctctccga ggaggccaag gacgacaagg
     2341 cccgcctgga ggcggaccgc atctggatcg tcgacccgtt ggacggcacc cgggagtaca
     2401 gcgagcctcc ccgggtggac tgggccgtgc acatcgccct cgtcgaccgg gcgggcactc
     2461 cactggccgg ggccgtggcc ctccccggac gagccctggt cctgggcacc gacccgccgg
     2521 tcgtcccggc cgagcgtcag gccggcccgc cccgagtcgt catcagccgc acccggcccc
     2581 cggccgaggc gttggccgtc gccgaggccc tgagcggcga ggtggtcgag atgggatcgg
     2641 ctggggccaa ggccatggcc gtggtactcg gcgaggtgga cgtctacgtc cactccggcg
     2701 gccagtacga gtgggactcc tgcgcgccgg tcgcggtggc ccgtgcggcc ggcctgcaca
     2761 ccagccgcat ggacggctcg ccgctggtct acaaccagga cgacgtgtac ctccccgacc
     2821 tcctcatctg ccggaccgag tacgtccagc cggtcctcga cctcctgaac tgaggcggcg
     2881 tggagctcaa ggtcgtccgc tac
//
LOCUS       DASTFV010000616         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2366612, whole genome shotgun sequence.
ACCESSION   DASTFV010000616 DASTFV010000000
VERSION     DASTFV010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2366612"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VFP02_11280"
     CDS             complement(<1..633)
                     /locus_tag="VFP02_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543660.1"
                     /translation="MRVLRPIIIGSLVSALFVGAVAPSAGADTVTDDGVAWLATRQQA
                     NGGFEVAGFDGFETPDAILAIAANAQTTATWSTSEALAAVAAVDNAGVTALDWVDAWV
                     DGELTDPPLIGQEAKLILQVAVPLGFDPTDFDPGDDDAPGAGVDLTAGLDENAPALFN
                     SFLFARLAESRLGQNVHQADLQVVCEAEKATGGGWSFDGDPAGANSADLDT"
     regulatory      complement(711..847)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            948..2060
                     /locus_tag="VFP02_11285"
     CDS             948..2060
                     /locus_tag="VFP02_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HET9543661.1"
                     /translation="MSTVPRFHAVTWLAWALAAVVIVQLASSPLYVTLVLAASALVAG
                     AHRISSTGAFRLLVFAGIVMALIRVVLTALTTHAVGTVLFTLPEATLPRILGGFTIGG
                     TIEKEVVLQSLAEGYAIVALLGVFGAFNSVVSHHELLGSAPRAFHEAGLVVTIAAAFV
                     PSTVASWQDAREADRARTGGIVRRRGRLARTVVPVLETGMERSLRLADSMDSRGFARH
                     PPGPADTASAWGALLALTALAGSFVALVGRRSTLAAVLATTGAVVLVSAVVLASKASP
                     RQRYRPRALTGEDLFIMATTALALTLVAVAAAGGEDSLTWSASPLHSPDLAPLPAVGI
                     LLLAVPALLPTQPVQDPESPTATAPSAARTVQEVVT"
     gene            2057..>2704
                     /locus_tag="VFP02_11290"
     CDS             2057..>2704
                     /locus_tag="VFP02_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9543662.1"
                     /translation="MSAVVTFAGVAFAYADEEPALVDIDLTVAAGDVLLVVGPSGSGK
                     STLLRAVNGLVPHATGGHFRGTVTVAGRSTRDHRPRDRADTVGFVHQDPEAQFVVDEV
                     EHDIAFSLENLGMEPAAMRRRVEEVLDALGIAHLRHRSPTTLSGGERQRAAVAGALAA
                     APDVLVLDEPTSELDPQGADDVLAAVTRLNADLGTTILLAEHRLERAAPMADRAVS"
BASE COUNT          335 a         1054 c          912 g          403 t
ORIGIN      
        1 cgtgtcgagg tcggccgagt tcgcccccgc gggatctccg tcgaagctcc agccccctcc
       61 cgtcgccttc tccgcctcac acaccacctg aaggtcagcc tggtggacgt tctggcccaa
      121 ccgcgactcg gccagccggg cgaacaggaa cgagttgaag agcgctggtg cgttctcgtc
      181 gaggccggcg gtcaggtcga cgccggcacc aggagcgtcg tcgtcacccg ggtcgaagtc
      241 cgtcgggtcg aagcccagcg gcacggcgac ctggaggatg agcttcgcct cctggccgat
      301 gagcggcggg tcggtgagct cgccgtcgac ccatgcgtcg acccagtcga gcgccgtgac
      361 gccggcgttg tccaccgcag cgacggcggc caaggcctcc gacgtcgacc aggtggccgt
      421 cgtctgggcg ttggccgcga tggccaagat cgcgtccggg gtctcgaacc cgtcgaatcc
      481 ggcgacctcg aagccaccgt tcgcctgctg tcgggtcgcc agccaggcca ccccgtcgtc
      541 ggtgaccgtg tccgcaccgg ctgacggcgc gaccgcgccg acgaacaggg cggagacgag
      601 ggagccgatg atgatcggcc gaagtacacg catgacaaac ctccgtgggg ccccgttccg
      661 cgggggccct catggggggc cactccctgg gcggcacccg aaacgggcgt cggcgaaccg
      721 acgggagtgg cgggcatcct ggctcacggc ccgagggccg cacacagttg cgggtcagcg
      781 tcggtctcgc accgacttcc cccgccccgg cagtgttgct accgggaccg cccacgacct
      841 ccgagccgat gggcgaccgc tccgccgggc acggtaggcg ggtctcggcc ggagcgtcaa
      901 ccgccgcgaa gaggcgggca cgggcgcccc gcggcatcct cttgccgttg agcaccgtcc
      961 cccgcttcca cgccgtgacc tggctggcct gggccctggc cgccgtggtc atcgtgcagc
     1021 tcgcctccag tcccctgtac gtgaccctgg tcctggccgc cagtgccctc gtggccggcg
     1081 cccaccgcat cagctcgacg ggggcattcc ggctgctggt gttcgccggg atcgtgatgg
     1141 ccctgatccg ggtcgtcctc accgcgctga ccacccacgc cgtcggcacc gtgctgttca
     1201 cgctgcccga ggccaccctg ccccgcatcc tcggcggctt caccatcggt ggcaccatcg
     1261 agaaggaggt cgtgctccag tccctggccg agggctacgc catcgtcgcc ctgctcggag
     1321 tattcggagc cttcaacagc gtggtgtccc accacgagct gctgggcagc gccccccgag
     1381 ccttccacga ggcggggctg gtggtgacca tcgccgccgc cttcgttccc tcgaccgtgg
     1441 cctcgtggca ggacgcccgg gaggcggatc gggcccgcac cggtgggatc gtccgtcgcc
     1501 ggggtcgcct ggcccgcacg gtcgtccccg tcctcgagac cggcatggaa cggtcgttgc
     1561 gcctggccga ctcgatggac tcccgtggct tcgcccgtca cccccctggt ccggccgaca
     1621 ccgcgtcggc gtggggggcc ttgctggccc tgacggccct ggccggctcg ttcgtcgcgc
     1681 tggtgggccg acgctcgacc ctggccgcgg tcctggccac gacgggcgcc gtcgtgctgg
     1741 tgagcgcggt cgtcctcgcg tcgaaggcct cgcctcgtca gcgctaccgc ccccgagccc
     1801 tcaccgggga ggaccttttc atcatggcga cgaccgcgct ggcattgacc ctggtggccg
     1861 tcgccgcggc aggtggcgag gactcgctga cctggtcggc ttcgcccctg cactcgccgg
     1921 acctggcccc cctccccgca gtcgggatcc tcctgctcgc cgttcccgct ctcctcccca
     1981 cccagccggt tcaggatccc gaatccccta ccgccacggc cccgagcgcc gccaggacgg
     2041 tccaagaggt ggtgacgtga gtgccgtcgt gaccttcgcc ggggtggcct tcgcctacgc
     2101 cgacgaggag ccggccttgg tcgacatcga cctgacggtc gccgccggcg atgtcctcct
     2161 ggtcgtcggg ccgtccggat caggcaagag caccctgctg cgggccgtca acggcctcgt
     2221 cccccacgcc acgggtggcc actttcgcgg gaccgtgacc gtcgccggtc gcagcacccg
     2281 cgaccaccgt ccccgcgacc gtgccgacac cgtcggcttc gtccaccagg accccgaggc
     2341 ccagttcgtc gtcgacgagg tggagcacga catcgccttc tcgctcgaga acctcggcat
     2401 ggaacccgcc gccatgcgcc gccgcgtcga ggaggtgctc gacgccctgg gcatcgccca
     2461 cctgcgtcac cgctccccca ccaccctctc cggtggcgag cgccagcggg ccgcggtggc
     2521 cggcgccctg gctgccgccc ctgacgtcct cgtcctggac gagcccacca gcgagctcga
     2581 tccccagggg gcggacgacg tgctcgccgc ggtgacccgg ctgaacgccg acctgggcac
     2641 cacgatcctg ctggcggagc accgcctcga gcgggccgcg ccgatggccg atcgggccgt
     2701 cagc
//
LOCUS       DASTFV010000617         3884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2368346, whole genome shotgun sequence.
ACCESSION   DASTFV010000617 DASTFV010000000
VERSION     DASTFV010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2368346"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1269)
                     /locus_tag="VFP02_11295"
     CDS             complement(1..1269)
                     /locus_tag="VFP02_11295"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET9543663.1"
                     /translation="MPESVTITDNRTGESIEVPITNGGVDASAWAKLLPNVWFLDPAF
                     STTAATESAITELDGEAGILRYRGYPIEQLAERATYLEVSYLLIHGELPNRAQLDTWI
                     HEITTHTFIHENIRKRFLEGFHHDAHPMGILVATVAGLSTFYPEAKAIDDPAVRLMQI
                     TRLIAKMPTLAAGAYRFSQGMPFVYPDNSLSFTENFQSMMWKIAEPRYQPDPVLSRAL
                     DVLFILHADHEQNCSTTAMRVVGSAHADPYIATSAAAAALYGPRHGGANEAVIKMLEE
                     IGGYDDVEPYIAKVKAGETRLQGFGHRVYKNYDPRATIIKRTADEVFRVTGPNPLLDI
                     ALKLEEVALEDDYFISRKLYPNVDFYSGLIYQAMGFPQEMFPVLFAIPRVSGWLAHWQ
                     EMLQQNQRISRPRQVYVGTPSRDFVPLHQR"
     gene            complement(1300..2226)
                     /locus_tag="VFP02_11300"
     CDS             complement(1300..2226)
                     /locus_tag="VFP02_11300"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9543664.1"
                     /translation="MDLRQLNALLAVAEHGGFSAAARALHTVQSNVSTHVARLEKELG
                     ATLIDRATGRPTKEGEAVLTRAIRIRAELEALSSDVASLGARVAGTARLGMIGTTARW
                     LAPLLFEALEEHYPEVRMVLVDATTTSLLPRVDRGDLDLAIVNLPAESMESQAEPLFD
                     EDRILLVPPDHPLGAAPTVALRALADYDLLLEPVGTAFRDELDVQAAAVGVRLRARAE
                     IDGMRLLASLVIAGFGAAVLPASAVPAVPPRADAPWRVIAVDGLQPRTVGLAQRRRGL
                     LSSPAAAVRDLLVDVVATGVAERPGLHPVTPT"
     gene            2454..3875
                     /locus_tag="VFP02_11305"
     CDS             2454..3875
                     /locus_tag="VFP02_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HET9543665.1"
                     /translation="MAGAAFFDLDRTLLMGASGPAISVALREAGVVTAPKIPGEGLVF
                     KVFDVIGETIPAMLLTRQAARMAKGWDQATVQAAAKNAADELIDRVLPYARLLIAQHR
                     AEGRPVVMATTTPVDLVTPLADLLELDDVVATRYGVDGNGGYDGTIDGEFVWGPGKLA
                     AVRAWAEPRGILLRDSFAYSDSFYDIPLLSAVGHPTAVNPDLRLAAVATVRRWPALHL
                     DVPPGVPKLGGLEPQQVLQAFARPELFPFARFHIEGVEHIPSKGGGLVCGNHRSYFDP
                     LVMAMTLSQKGRPVRFLGKKEVFDAPVVGQIAKAMGGIRVDRGTGSDEPLEAAADALS
                     AGQLVALMPQGTIPRGPAFFDPELQGRWGAARLAAKTRVPVIPVGLWGTEKVWPRNAR
                     VPNVLNITDPPEITVRVGPEVELTYEDDDADTKSIMAAIVDLLPAEARTYREPTGEEL
                     AQTYPSGWTGDPDTESERRPGSD"
BASE COUNT          579 a         1308 c         1413 g          584 t
ORIGIN      
        1 ctagcgctgg tggaggggga cgaagtcccg gctgggcgtc cccacgtaga cctggcgggg
       61 ccgggagatg cgctggttct gctgcagcat ctcctgccag tgggccagcc agccggacac
      121 ccgcggaatg gcgaagagga ccgggaacat ctcctgggga aagcccatcg cctggtagat
      181 gagaccggag tagaagtcga cgttcgggta gagcttgcga ctgatgaagt agtcgtcctc
      241 gagggcgacc tcctccagct tcagggcgat gtccagcaac gggttcgggc cggtgacccg
      301 gaagacctcg tctgcggtcc tcttgatgat cgtcgcccgg gggtcgtagt tcttgtagac
      361 ccgatggccg aagccctgca gccgggtctc gcccgccttc accttggcga tgtacggctc
      421 gacgtcgtcg tagccgccga tctcctcgag catcttgatg accgcctcgt tggccccgcc
      481 gtggcggggg ccgtagagag ccgcggccgc cgctgaggtg gcgatgtagg ggtcggcgtg
      541 ggcactgccg acgacccgca tggcggtggt cgagcagttc tgctcgtggt cggcgtggag
      601 gatgaagagc acgtccaggg cccgggacag caccgggtcg ggctggtagc ggggctcggc
      661 gatcttccac atcatcgact ggaagttctc ggtgaagctc agggagttgt ccgggtagac
      721 gaagggcatg ccctggctga accggtaggc gccggcggcc agcgtcggca tcttggcgat
      781 caggcgagtg atctgcatca gccggaccgc aggatcgtcg atggccttcg cctcggggta
      841 gaaggtggag aggccggcca cagtggccac caggatcccc atcgggtggg cgtcgtggtg
      901 gaagccctcg aggaacctct tgcggatgtt ctcgtggatg aacgtatggg tcgtgatctc
      961 gtggatccag gtgtcgagct gggcccggtt gggcagctcg ccgtggatga gcaggtacga
     1021 gacctcgagg taggtggccc gctcggccag ctgctcgatg gggtagcccc ggtagcggag
     1081 gatgccggcc tcgccgtcca gctcggtgat ggcgctctcg gtggccgccg tcgtgctgaa
     1141 ggcgggatcg aggaaccaga cgttgggcag gagcttcgcc cacgccgacg cgtcgacgcc
     1201 accgttggtg atcgggacct cgatcgattc tcccgtgcgg ttgtcggtga tcgtcacgga
     1261 ctcgggcatg aaggcgaggc tacgtcctcc cacgggccct cacgtagggg tgacgggatg
     1321 cagtccaggc cgctcggcga cacccgtcgc gacgacgtcg accaggaggt cgcgcacggc
     1381 ggctgcgggt gacgagagca gcccccgccg gcgctgggcc aggccgacgg ttcggggttg
     1441 gaggccgtcg acggctatga ctcgccacgg cgcatcggcc cggggtggga cggcagggac
     1501 ggcggaggcg gggagcacgg cggcaccgaa gccggcgatg accagcgagg ccagcaggcg
     1561 catgccgtcg atctcggccc tggcccgcag ccggacaccg acggccgcag cctggacgtc
     1621 caactcgtcc cgaaacgccg tcccgacggg ctccagcagc aggtcgtagt cagccagcgc
     1681 ccgcagggcc accgtggggg cggcgccgag gggatgatca ggagggacga gcaggatgcg
     1741 gtcctcgtcg aagagcggct cggcctggct ctccatgctc tcggcgggga ggttgacgat
     1801 ggccaggtcg aggtcgcccc gatcgacccg ggggagcagg gaggtcgtgg tggcgtccac
     1861 cagcaccatc cggacctcgg ggtagtgctc ctcgagggcc tcgaacagga gcggggccag
     1921 ccagcgggcc gtcgtgccga tcatgccgag ccgggccgtg ccggcgaccc gggcgcccag
     1981 ggaggccacg tcggaggaga gggcctcgag ctcggcccgg atgcggatcg cccgggtcag
     2041 caccgcctcg ccctccttgg tcggccggcc cgtcgcccgg tcgatgaggg tggctcccag
     2101 ctccttctcg agtcgggcca cgtgggtgga gacgttggac tggaccgtgt gcagggcccg
     2161 ggcggcggcg gagaagccac cgtgctcggc cacggccagg agggcgttga gctggcgcag
     2221 gtccatcgcg aagagtgatg ttacccatct gtaacaactg cttgaccgat ctcgtgaaca
     2281 ggatcacaat ggcgcgacaa agcccagccg aggaatcgtc cttcccccaa ggaacgactc
     2341 ggccgggtcc cgagggtcgg tcccccccat agccctcggg acagagcaat gcaggggccg
     2401 gctcttcccc ccggagccgg tcccttttgc tgtccggggc tagcttgctg ggcatggctg
     2461 gcgcggcgtt cttcgatctg gaccggacgt tgctgatggg ggcgtcgggg ccggcgatct
     2521 cggtggccct acgcgaggcc ggggtcgtca cggccccgaa gatcccgggc gaggggctgg
     2581 tcttcaaggt cttcgacgtc atcggcgaga ccatcccggc gatgctgctg acccgccagg
     2641 cggcgcgcat ggccaagggc tgggaccagg ccaccgtgca ggccgcggcc aagaacgcgg
     2701 ccgacgagct catcgaccgg gtgctgccgt acgcccggct cctcatcgcc cagcaccggg
     2761 ccgaagggcg acccgtcgtc atggccacca cgaccccggt cgacctggtc acgccgctcg
     2821 ccgatctcct ggagctcgat gacgtcgtgg ccacccggta cggcgtggac ggcaacggcg
     2881 gttacgacgg gacgatcgac ggcgagttcg tgtgggggcc ggggaagctc gccgctgtac
     2941 gggcgtgggc cgagccccgc gggatcctcc tccgggactc cttcgcctac tccgacagct
     3001 tctacgacat cccgctgctg tccgccgtcg gccatcccac tgcggtcaac cccgacctgc
     3061 gcctcgccgc cgtggccacc gtgcgccggt ggcccgccct gcacctcgac gtcccacccg
     3121 gcgtgcccaa gctgggcggc ctcgagcccc agcaggtgct ccaggccttc gcccgccccg
     3181 agctcttccc gttcgcccgc tttcacatcg agggcgtcga gcacatcccg tcgaagggcg
     3241 gtggcctcgt ctgcggcaac caccggagct acttcgaccc cctggtcatg gccatgaccc
     3301 tcagccagaa gggccgtccg gtgcgcttcc tgggcaagaa ggaggtcttc gacgccccgg
     3361 tcgtgggtca gatcgccaag gccatgggcg gcatccgggt cgaccggggg acgggctccg
     3421 acgagcccct cgaggccgcg gccgacgccc tgtcggccgg ccagctggtc gcgctgatgc
     3481 cccaggggac gattccccgt ggtcccgctt tcttcgaccc cgagttgcag ggacgttggg
     3541 gcgccgcccg cctcgccgcg aagacccggg tgccggtgat cccggtggga ctctggggca
     3601 cggagaaggt ctggccccgc aacgccaggg tccccaacgt cctgaacatc accgacccgc
     3661 ccgagatcac cgtgagggtc ggccctgagg tcgagctgac ctacgaggac gatgacgccg
     3721 acaccaagtc gatcatggcc gccatcgtcg atctgctccc ggccgaagcc cggacctacc
     3781 gcgagcccac cggcgaggag ctcgcccaga cctatccgtc gggctggacc ggcgaccccg
     3841 acaccgagag cgagcgccgc cccggctccg actgatccgg tcag
//
LOCUS       DASTFV010000618         4041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2372890, whole genome shotgun sequence.
ACCESSION   DASTFV010000618 DASTFV010000000
VERSION     DASTFV010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2372890"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..779)
                     /gene="coaE"
                     /locus_tag="VFP02_11310"
     CDS             complement(156..779)
                     /gene="coaE"
                     /locus_tag="VFP02_11310"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET9543666.1"
                     /translation="MLLVGLTGGIASGKTTVSAMLAERGAEVIDADHIARQVVLPGMP
                     AWCKIREHFGPGVLHPDGQIDRQALADIVFADNTKLALLNEITHPAIFARIADLLEAH
                     HDQDVVVVLDAALLIEAGLAEGVEVVIVTHSPHEIQVERLAAKGVGKRDASNRIAAQL
                     EPEKRLARADIVVDNSGSLEKLGRRVDEVWEELQRLLWRPSDGPPGS"
     gene            complement(855..2384)
                     /gene="rpsA"
                     /locus_tag="VFP02_11315"
     CDS             complement(855..2384)
                     /gene="rpsA"
                     /locus_tag="VFP02_11315"
                     /inference="COORDINATES: protein motif:HMM:NF005911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET9543667.1"
                     /translation="MTESTERPTATEQDVPGVTTQVTQNDVGSAEDLAAAYEESLKAF
                     EEGDIVDGYVVKVDRDEVLLDIGYKSEGVIPSRELSIKHDVDPNEVVSLGDHIEALVM
                     QKEDKDGRLILSKKRAQYERAWGTIEKKKENDEIVEGTVIEVVKGGLILDIGLRGFLP
                     ASLVEMRRVRDLHPYVGRTLEAKIIELDKNRNNVVLSRRAWLEETQSATRRAFLHTLR
                     KGEVRKGVVSSIVNFGAFVDLGGVDGLVHVSELSWKHIDHPSEVVEVGQEVEVEVLDV
                     DLERERVSLSLKATQEDPWRQFARSHQIGELIPGRVTKLVPFGAFVRVDDGIEGLVHI
                     SELAERHVDIPEQVVSVGEEITVKVIDIDLDRRRISLSLKQAAQDQAQVTAPEDELAL
                     EEGAYQGYEGEYAEGAYVEGAYAEGDYADGSYVYDEQGNPIGVMPGTAMAEAFQEAGF
                     YEGTYEGEGQATEEQPAQDGEGVVAQEAEAGDAGQAQQAAGDDTLENIVEDLKRKNAS
                     E"
     gene            2587..3324
                     /locus_tag="VFP02_11320"
     CDS             2587..3324
                     /locus_tag="VFP02_11320"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HET9543668.1"
                     /translation="MTEPLYQHDAYLAAFDAQVTGVEPDGVVLDRSAFFPGGGGQPPD
                     TGVLEVEGVELAVTGAQRREGRVVLLVDGQPPPAGAKVRGRLDWERRHALMRTHTALH
                     MLSGVIGRDYGALVTGGNFEEQKARMDFELASMSAEFAEAVERALRVEVDADRPVHVS
                     FLSPEEFASRPELIRTKANLVPQGLKEVRVIDIEGLDRQADGGTHVASTGEVGTIYVV
                     GHQSKGKGNKRLRIALDPIPPAPSRSE"
     gene            3321..3953
                     /locus_tag="VFP02_11325"
     CDS             3321..3953
                     /locus_tag="VFP02_11325"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9543669.1"
                     /translation="MSQPDWLGWVARLQAIAQAGLELSDGPYDLERYKAVRALATEIA
                     AAHLELPSEAERLALDELLAGDSGYPTPKVDVRALVRRDGEVLLVRERADGRWSLPGG
                     WADVGWSPAAMVEREVAEESGFQVRARRLLALWDRSRHNPGPFPHSVYKVAVACDLLG
                     GEPRPSIETLDVGWFPPHRLPELSTGRITAAQIARLIELDDHPELPPDLD"
BASE COUNT          554 a         1440 c         1419 g          628 t
ORIGIN      
        1 ccgttggcgg cggccacggc cacgaaccgt ccctggaagc tcggcgcagg cgccagcagg
       61 gtgtcgccgg catcgaacag cacacagtcg accgggtcgg gaaggggggg cagacgactc
      121 atattgcgca ggctacagcc ctcgaggtgc cgcagtcacg agccgggggg tccatccgag
      181 ggccgccaca gcaggcgctg gagctcctcc cagacctcgt cgacgcggcg gccgagcttc
      241 tccagcgagc cgctgttgtc gaccacgata tcggccctgg ccaggcgttt ctccggctcg
      301 agctgggcgg cgatgcggtt ggaggcgtcc cgcttcccca cccccttggc ggccagccgc
      361 tcgacctgga tctcgtgggg ggagtgggtg acgatcacca cctcgacgcc ctcggccagc
      421 ccggcctcga tcagcagggc cgcgtccagg accacgacca cgtcctggtc gtggtgggcc
      481 tcgaggaggt cggcgatgcg ggcgaagatc gccggatggg tgatctcgtt gagcagggcc
      541 agcttggtgt tgtcggcgaa gacgatgtcg gccagggcct ggcggtcgat ctggccgtcg
      601 ggatgcagca cccctggacc gaaatgctcg cggatcttgc accaggccgg catgcccggc
      661 agcaccacct gcctggcgat gtggtcggcg tcgatgacct cggcgccccg ttcggcgagc
      721 atggccgaga ccgtggtctt gcccgaggcg atgcctcccg tcagcccgac cagcagcatg
      781 tctgccgccc cctcgagata ggtccccgct gaccggccga cgtgccggcg acgccgcgag
      841 cccggccggg cccgctactc gctggcgttc ttgcgcttga ggtcctcgac gatgttctcg
      901 agggtgtcgt cgcccgccgc ctgctgcgcc tggccggcgt cgccggcctc ggcctcctgg
      961 gcgaccacgc cctcgccgtc ctgcgccggc tgctcctcgg tcgcctggcc ctcgccctcg
     1021 taggtgccct cgtagaagcc ggcctcctgg aacgcctcgg ccatggccgt gcccggcatc
     1081 acgccgatgg ggttgccctg ctcgtcgtag acgtagctgc cgtcggcgta gtcgccctcg
     1141 gcgtaggcac cttcgacata ggcaccctcg gcgtactcgc cctcgtagcc ctggtaggcg
     1201 ccctcctcca gggccagctc gtcctccggg gcggtgacct gcgcctggtc ctgggcggcc
     1261 tgcttgagcg acagggagat gcggcggcgg tccaggtcga tgtcgatcac cttgacggtg
     1321 atctcctcgc cgacgctgac gacctgctcg gggatgtcga cgtggcgctc ggccagctcc
     1381 gagatgtgga ccaggccctc gatgccgtcg tcgacgcgga cgaaggcgcc gaacggcacc
     1441 agcttggtga cccggcccgg gatgagctcg ccgatctggt gggagcgggc gaactggcgc
     1501 cacgggtcct cctgggtcgc cttgagcgac agcgagaccc gctcgcgctc caggtcgacg
     1561 tccaggacct cgacctcgac ctcctggccg acctcgacca cctcggaggg gtggtcgatg
     1621 tgcttccagg acagctcgga cacgtgcacc aggccgtcga cgccgcccag gtccacgaag
     1681 gcgccgaagt tgacgatgct ggagacgacg cccttgcgga cctcgccctt gcgcagggtg
     1741 tgcaggaacg cccggcgggt ggccgactgg gtctcctcca gccaggcccg gcgcgacagg
     1801 accacgttgt tgcggttctt gtcgagctcg atgatcttcg cctcgagggt gcgaccgaca
     1861 taggggtgca ggtcgcgcac ccggcgcatc tcgaccaggg aggcgggcag gaagccgcgc
     1921 aggccgatgt cgaggatcag gccgcccttg acgacctcga tgacggtccc ctcgacgatc
     1981 tcgtcgttct ccttcttctt ctcgatcgtg ccccaggccc gctcgtactg ggcccgcttc
     2041 ttggacagga tcaggcggcc gtccttgtcc tccttctgca tgaccagggc ctcgatgtgg
     2101 tcaccgagac tgacgacctc gttggggtcg acgtcatgct tgatggacag ctcgcgggag
     2161 gggatgacgc cctcggactt gtagccgatg tcgagcagca cctcgtcgcg gtcgaccttc
     2221 accacgtagc cgtcgacgat gtcaccctct tcgaaggcct tcagcgactc ttcgtaggcg
     2281 gccgcgaggt cctcggcgga gccgacatcg ttctgcgtca cctgggtggt taccccaggc
     2341 acgtcctgct cggttgcggt gggtcgctcg gtggactcgg tcaactgctc gtctcctaga
     2401 ctgtcggaca gaaggctccc cctgcagaca ttttcgtggc ggtgaggcga cgcgatgagc
     2461 gtcagaacac actggcagaa aagcccacag tggttcggca gatggtagcc gtccctgctt
     2521 caccacgtca acggtgcatg cgcgtatgac ctgcgagttt atggtccttc gagaaagggt
     2581 ggtcaagtga ccgagccgct gtatcagcac gacgcctacc tcgccgcctt cgatgcccag
     2641 gtcaccgggg tcgaacccga cggtgtcgtc ctcgaccgga gcgcgttctt tccgggcggg
     2701 ggcggccagc cgcccgacac cggcgtcctc gaggtcgagg gggtcgagct ggccgtcacc
     2761 ggcgcccagc gccgcgaggg ccgggtcgtg ctgctggtcg acggccagcc gccgcccgcc
     2821 ggcgccaagg tcagggggcg gctggactgg gaacgccgcc acgccctgat gcgcacccac
     2881 accgccctgc acatgctgtc gggggtgatc ggccgcgact acggggcgct ggtcaccggg
     2941 ggcaacttcg aggagcagaa ggcccggatg gacttcgagc tggcctcgat gagcgccgag
     3001 ttcgccgagg cggtcgagcg ggcgctgcgg gtcgaggtcg acgccgaccg gccggtgcac
     3061 gtctcgttcc tgtcgccaga ggagttcgcc tcccggcccg agctgatccg gaccaaggcc
     3121 aacctcgtcc cccaggggct gaaggaggtc cgggtgatcg acatcgaggg ccttgaccgc
     3181 caggccgacg gcggcaccca cgtcgcctcg accggggagg tcgggaccat ctacgtggtc
     3241 ggccaccagt ccaagggcaa gggcaacaag cggctgcgca tcgccctcga cccgatcccc
     3301 cccgccccgt cccgctcgga gtgagccagc cggactggct gggctgggtc gcccgcctcc
     3361 aggccatcgc ccaggcgggg ctggagctgt ccgacgggcc gtacgacctg gagcggtaca
     3421 aggcggtgcg cgccctggcc accgagatcg ccgccgccca cctggagctg ccgtcagagg
     3481 ccgagcggct ggccctggac gagctcctgg ccggggacag cggctacccg acgcccaagg
     3541 tcgacgtgcg cgccctggtc cggcgcgatg gtgaggtgct gctggtccgg gaacgggccg
     3601 acggccgctg gtcgctgccc ggcggctggg ccgacgtcgg ctggtccccg gcggccatgg
     3661 tggagcggga ggtcgccgag gagtccgggt tccaggtgcg ggcccgccgg ctcctggccc
     3721 tgtgggaccg gtcgcggcac aacccgggcc cgttcccgca ctcggtctac aaggtggcgg
     3781 tcgcctgcga cctgctcggc ggcgagcccc gccccagcat cgagaccctc gatgtcggct
     3841 ggttcccccc gcaccggctg cccgagctgt ccaccggccg catcaccgcc gcccagatcg
     3901 cccgcctgat cgagctggac gaccacccgg agctgccgcc ggacctggac tagcccatca
     3961 gcgcctcggg cggctggccg ccagccgggt cccggcgacg gccccgacca gggcggccag
     4021 ggcgacgagg accagccagg c
//
LOCUS       DASTFV010000619         2925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2373972, whole genome shotgun sequence.
ACCESSION   DASTFV010000619 DASTFV010000000
VERSION     DASTFV010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2373972"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VFP02_11330"
     CDS             <1..663
                     /locus_tag="VFP02_11330"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HET9543670.1"
                     /translation="EGTALLKKVLDAYRQPDPGGMPGGGRSKAQTDYDALMAALATAI
                     DGKAGRPKRTADIVVSYELLLRMQPVDLVNVRCDLEGAGPVPLSMLRRLAADSALGRI
                     IASSDGVPLELGTEVRWFNAQHRRALRLRDQTCVWPGCDLPGEWSEMDHALEAALGGP
                     TSLANARFLCVRHHHLRHKGWKVTYDPVSRHCSITSPLGITWTGDGDVILPDDPDPPP
                     RE"
     gene            685..1245
                     /locus_tag="VFP02_11335"
     CDS             685..1245
                     /locus_tag="VFP02_11335"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET9543671.1"
                     /translation="MGEPGGSGPVGPYPPGVEGPEAEDEYDRLRRRVLWSLPYGLYIV
                     GSRAGERRNGMTINFVSQVSMNPKLMGIGVEAEAVTHELITEGGVFALNTISREDRAI
                     VRKFTKPVEVDAEASTLNGFPFHDGLTGAPILDQAVAYVDCEVRQALDLGNHTLFIGE
                     VVDAGFQSDEDTEVLRMEDTRLNYGG"
     gene            1278..2525
                     /locus_tag="VFP02_11340"
     CDS             1278..2525
                     /locus_tag="VFP02_11340"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9543672.1"
                     /translation="MDIAGPQITSFPETGVGVDELLAEIDEERAGDIDWRAGRAFSLV
                     YHHHDDVLEGLQHEVADRFLHENALNPFAYRTLPRMEQEVVLMAGDLFGGEPGAGSLT
                     SGGTESIFLAVQVARDHARENGIAEPELLTANTAHPAFAKAARYLDVAHRKVPVAEDL
                     RLHGEQVAEAIGGQTALVVGSAPCYPFGVIDHITDIAAVAQDAGVLCHVDACLGGWLL
                     PWWERLGETVPPWDLRVDGVTSLSADVHKYGYATKGASTVLYASRDLYRHQFFLYDEW
                     PGGLYGSSTSAGTRGGVPIAEAWAAVRHLGAEGYLRLAEVVRGATHGFQTGIEGIDGL
                     DITGDPDLSVFEFGSTTLDIGGVADVMDDRGWHLDRQQGGLHVMVSPGHADVVDRFVA
                     DLTEAVDEHEASRGREATYGGTV"
     gene            2557..>2925
                     /locus_tag="VFP02_11345"
     CDS             2557..>2925
                     /locus_tag="VFP02_11345"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HET9543673.1"
                     /translation="MLTGGSSRRMGTDKALLVVDGQPLAVIVADALRTAGADRVVAVG
                     GDLDGLRALGLDAVPDLHAGEGPLGGILTALDATTEDVVVVLACDLPGADPATITTVV
                     EALGDADMAAPLRDGRHELLH"
BASE COUNT          434 a         1076 c         1000 g          415 t
ORIGIN      
        1 gaaggcacgg ccctgctgaa gaaggtcctc gacgcctacc gccagcccga cccggggggc
       61 atgcctggtg gcgggaggtc gaaggcccag accgactacg acgccttgat ggccgcgctg
      121 gccactgcca tcgacggcaa ggccggtcgg cccaagcgga ccgctgacat cgtggtctcc
      181 tacgagctcc tcctccggat gcagccggtt gacctggtca acgtccgctg cgatctggag
      241 ggcgccggtc cggtgccgct ttcgatgctg cggcgcctgg ccgccgactc cgccctcggc
      301 cgcatcatcg cctcgagcga cggcgtgccc ctcgagttgg gcaccgaggt ccgctggttc
      361 aacgcccagc accgccgggc cctccgcctc cgggaccaga cctgcgtctg gcccggctgt
      421 gacctcccgg gcgagtggtc agagatggac cacgccctcg aagccgcgct cggcggcccg
      481 accagcctgg ccaacgcccg cttcttgtgc gtgcgccatc accacctccg ccacaagggc
      541 tggaaagtca cctacgaccc ggtgagcaga cactgctcga tcaccagtcc cctcggcatc
      601 acgtggaccg gcgacggcga cgtcatcctc cccgacgacc ccgacccgcc accacgggag
      661 tgaccggccg gtaggttcca acggatgggt gagccgggtg gcagtggacc tgtcgggccg
      721 tatccgccag gcgtcgaggg accggaggcc gaggacgagt acgaccgcct ccgtcgccgc
      781 gtcctctggt cgctgcccta cgggctgtac atcgtcggca gccgcgcggg cgagcggcgc
      841 aacgggatga ccatcaactt cgtctcccag gtgtccatga acccgaagct gatgggcatc
      901 ggcgtcgaag ccgaggccgt gacccacgaa ttgatcaccg aaggtggcgt cttcgccctc
      961 aacacgatca gccgggagga ccgggcgatc gtgcgcaagt tcaccaagcc cgtcgaggtc
     1021 gacgccgaag cctcgaccct caacggcttc ccgttccacg acggcctcac cggcgccccg
     1081 atcctggacc aagcggtcgc ctacgtggac tgcgaggtcc gccaggccct cgacctgggc
     1141 aaccacacgc tcttcatcgg cgaggtggtc gacgccggct tccagtccga cgaggacacc
     1201 gaggtcctcc gcatggagga cacgcggctg aactacggcg gctgaccctt cacgagccga
     1261 cccgtagcat cggcggcgtg gacatcgccg ggccccagat cacgagcttc cccgagacgg
     1321 gggtgggggt cgacgagctg ctggcggaga tcgacgagga acgcgccggc gacatcgact
     1381 ggcgggctgg tcgggccttc agcctcgtct accaccacca cgatgacgtg ctcgagggcc
     1441 tgcagcacga ggtagccgat cgcttcctgc acgagaacgc cctgaacccg ttcgcctacc
     1501 ggaccctgcc ccgcatggag caggaggtcg tcctgatggc cggggacctc ttcggtggcg
     1561 agccgggcgc cgggtcgctg acgtcgggcg ggaccgagag catcttcctg gccgtccaag
     1621 tggctcgaga ccacgcccgg gagaacggca tcgccgagcc cgagcttctc acggcgaaca
     1681 cggcccaccc ggccttcgcg aaagcggcca ggtacctgga cgtggcccac cgcaaggtcc
     1741 ccgtggccga ggacctccgg ctgcacggcg agcaggtggc ggaggccatc ggcgggcaaa
     1801 cggccctcgt tgtgggctcg gccccctgct acccgttcgg cgtcatcgac cacatcaccg
     1861 acatcgccgc ggtggcccag gacgcgggcg ttctctgcca cgtcgacgcc tgcctcggtg
     1921 gctggctcct gccttggtgg gagcggctgg gcgagacggt gccgccgtgg gacctccgcg
     1981 tcgacggcgt gacctcgctg tccgcggacg tgcacaagta cggctacgcc acgaagggcg
     2041 cttcgacggt cctctacgcg tcccgcgacc tctatcgcca ccagttcttc ctctacgacg
     2101 agtggcccgg tggcctctac ggctcgtcca cgtccgccgg tacccggggc ggggttccga
     2161 tcgccgaggc ctgggctgcg gtgcgacacc tcggggccga gggctatctc cggctcgccg
     2221 aggtcgtacg gggcgccacc cacggattcc agaccgggat cgagggcatc gacggcctgg
     2281 acatcaccgg cgacccggac ctctccgtct tcgagttcgg ctcgaccacc ctggacatcg
     2341 ggggcgtggc cgacgtcatg gacgaccggg gctggcacct cgaccgccag cagggtgggc
     2401 tccacgtgat ggtctcgccc ggccatgccg acgtcgtgga ccgattcgtg gccgatctga
     2461 ccgaagccgt cgacgagcac gaggccagcc gcggccgcga ggccacctac ggcgggacgg
     2521 tctgatctgt ccgagcgacc gcccttcgcc ggggccgtgc tgaccggtgg gtccagccgg
     2581 cgcatgggaa ccgacaaggc cctcctggtc gtcgacgggc agccgctggc ggtcatcgtg
     2641 gccgacgccc tgcgcacggc gggcgccgac cgggtcgtcg ccgtcggtgg ggacctggac
     2701 ggtctcaggg cactcggcct ggacgccgtg cccgacctcc acgccggcga gggaccgctg
     2761 ggcgggatcc tcacggccct cgatgccacc accgaggacg tcgtcgtggt cctggcctgc
     2821 gacctgcccg gcgccgaccc ggccaccatc acgaccgtcg tggaggcgct gggcgacgcg
     2881 gacatggcgg cgccgctccg tgacggccgc cacgagctcc tgcac
//
LOCUS       DASTFV010000620         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2374245, whole genome shotgun sequence.
ACCESSION   DASTFV010000620 DASTFV010000000
VERSION     DASTFV010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2374245"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFP02_11350"
     CDS             complement(<1..193)
                     /locus_tag="VFP02_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543674.1"
                     /translation="MATGRTWEITKAYDELVELLRDFDDEFMTGPNHVADDEQLVVMG
                     YKWIFSILQVAMDVYLWGDA"
     gene            complement(197..1063)
                     /locus_tag="VFP02_11355"
     CDS             complement(197..1063)
                     /locus_tag="VFP02_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9543675.1"
                     /translation="MSERLDVRKTYKLYVGGQFPRSESGRTYPVSGPDGGLLAHAVLG
                     SRKDLRDAVKAARGAQAGWAGKTAYNRAQILYRVGEIMEGRRSQFVDELAAGGVDDPE
                     GEVDASIDRWVWYAGWADKLPSVLGGANAVAGPYFNFTVPEPTGVVGLIAPDEAPLLA
                     LVSRLAPIIVSGNTVVALASEALPLPAVTLSEALATSDVPGGVINILTGRRAELTPWF
                     AGHRDLNALDLTGVDDADLAADAERAAADSVMRVTRATGADADWRSSTAESPYAIEAF
                     LEHKTVWHPKGA"
     gene            complement(1060..>2226)
                     /locus_tag="VFP02_11360"
     CDS             complement(1060..>2226)
                     /locus_tag="VFP02_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9543676.1"
                     /translation="LQERSREFAVLETLDGGKPIKESRDVDLPLAAAHFFHYAGWADK
                     LDYAFAGRRGRPLGVAGQVIPWNFPMLMASWKLAPALATGNTVVLKPAETTPLTALLL
                     AEVLQDAELPPGVVNIITGAGATGSALVGHPGIDKVAFTGSTDVGKRIQTELAGTGKR
                     LTLELGGKAANIVFEDAALDQAVEGIVNGIWFNQGHVCCAGSRLLVQESVADLVLDRL
                     RTRLDTLRVGDPLDKNTDVGAINSQKQLEKIRELVASGVDEGAEIYQPECVLPDKGFF
                     HAPTIFTGVSQSHRIAREEIFGPVLSVLTFRTPDEAVEKANNTPYGLSAGVWTEKGSR
                     ILWMAERLRAGVVWANTFNQFDPASPFGGYKESGFGREGGRHGLAPYLQLERVA"
BASE COUNT          313 a          784 c          793 g          336 t
ORIGIN      
        1 cggcgtcgcc ccacaggtag acgtccatgg ccacctgcag gatcgagaag atccacttgt
       61 agcccatgac cacgagctgc tcgtcgtcgg ccacgtggtt gggaccggtc atgaactcgt
      121 cgtcgaagtc cctcagcagc tccacgagct cgtcgtaggc cttcgtgatc tcccaggtcc
      181 tgcccgtcgc cacgactcat gcccccttcg gatgccacac ggtcttgtgc tccaggaacg
      241 cctcgatggc atacggcgac tcggcggtcg acgaccgcca gtcggcgtcc gcgccggtgg
      301 cccgggtgac ccgcatgacg ctgtccgcgg ccgcccgctc ggcgtcggcc gccaggtcgg
      361 cgtcgtccac gccggtcagg tcgagggcgt tgaggtcccg gtggccggcg aaccacggcg
      421 tgagctcggc ccgcctaccg gtgaggatgt tgatgacccc gccgggcacg tcgctggtgg
      481 ccagtgcctc gctcagggtg acggcgggca gcggcagggc ctccgacgcc agggcgacga
      541 ccgtgttgcc ggagacgatg atcggcgcca gccgggacac gagggccagc agcggcgcct
      601 cgtcgggggc gatgagcccg accacaccgg tgggctcggg caccgtgaag ttgaagtacg
      661 gcccggccac ggcgttggcc ccgcccagca ccgacggcag cttgtcggcc catccggcgt
      721 accagaccca gcggtcgatc gaggcgtcga cctcgccttc cggatcgtcg acgccgccgg
      781 cggccagctc gtcgacgaac tgggaccggc gcccctccat gatctcgccg acccggtaga
      841 ggatctgcgc ccggttgtag gccgtcttcc cggcccagcc ggcctgggcc cctcgagccg
      901 ccttgaccgc gtcccgcagg tccttgcggg agccgaggac ggcgtgggcc agcagtccgc
      961 cgtcggggcc gctgaccggg taggtccgac cggactccga gcgggggaac tggcccccga
     1021 cgtagagctt gtaggtcttg cggacgtcca agcgctcgct cacgccaccc tctccagctg
     1081 gaggtagggg gccaggccgt ggcggccgcc ctcgcggccg aagccggact ccttgtagcc
     1141 gccgaaggga ctggccgggt cgaactggtt gaacgtgttg gcccacacga cacccgcccg
     1201 caggcgctcg gccatccaga ggatgcggct gcccttctcg gtccagaccc ccgccgacag
     1261 gccgtagggg gtgttgttgg ccttctcgac ggcctcgtcg ggcgtgcgga aggtcaggac
     1321 cgagaggacg ggcccgaaga tctcctcccg ggcgatccgg tgggactggg acaccccggt
     1381 gaagatcgtg ggggcgtgga agaagccctt gtcgggcagg acgcactcgg gctggtagat
     1441 ctcggccccc tcgtccaccc cggaggccac cagctcccgg atcttctcca gttgcttctg
     1501 gctgttgatg gccccgacgt cggtgttctt gtcgagcgga tcccccaccc ggagggtgtc
     1561 caggcgggtg cgcagccggt cgaggacgag gtcggcgacg gactcctgca cgagcaggcg
     1621 ggagccggcg cagcagacgt gaccctggtt gaaccagatg ccgttgacga tgccctccac
     1681 cgcttggtcc agggcggcgt cctcgaagac gatgttggcc gccttgccgc cgagctccag
     1741 ggtcaagcgc ttgccggtgc ccgccagctc ggtctggatc cgcttgccca cgtcggtcga
     1801 gccggtgaag gcgaccttgt cgatccccgg gtgcccgacc agcgccgacc ccgtcgcccc
     1861 ggcgcccgtg atgatgttga ccacgcctgg cggcagctcg gcgtcctgga ggacctcggc
     1921 cagcagcagc gcggtcaggg gcgtcgtctc ggccggcttg agcacgacgg tgttgcccgt
     1981 agccagcgcc ggggccaact tccagctggc catgagcatc gggaagttcc aggggatgac
     2041 ctggccggcg acgccgagcg gccgaccgcg acggccggca aaggcgtagt cgagcttgtc
     2101 ggcccagccc gcgtagtgga agaagtgcgc ggcggcgaga ggcaggtcga cgtcgcggga
     2161 ctccttgatc ggcttgcctc cgtcgagggt ctccaggacg gcgaactccc gcgaccgctc
     2221 ctgcag
//
LOCUS       DASTFV010000621         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2381216, whole genome shotgun sequence.
ACCESSION   DASTFV010000621 DASTFV010000000
VERSION     DASTFV010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2381216"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="VFP02_11365"
     CDS             complement(<1..1286)
                     /locus_tag="VFP02_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543677.1"
                     /translation="MPAAPAPPGRVRRFAELLALNGIIVAEPVLTALRQGADVFVSRR
                     AGRLDIVALTLLLVLGLPAVLFAIEELVGLVRRGRNGDQSRDRVHRILLGGLGGLVVV
                     RLLGDAGLPGPVTVLLAVAGGAVLWQAVTRWEGPRLWLQVLGVFPLLLGAWFLATDPV
                     RGLVLDPASTTGEAAAVGAPAPVVLVMLDEFPLASILDADDRIDPEQFPNLAHLADDA
                     TWFRNATGVSPTTPEAVPPILTGRYPDAVGVLPTAEEHPNNIFTLLQGTYGFHVQESV
                     TQLCPAHLCAEGALATGNPLAELTQDALDLWQRQLFGGGDDRQVDFAIRQSDPDAPTT
                     INEFAAGIGRGDRPELDVLHAVYPHQPWYHLPSGLTYEAPFIAEGLDQYAQYAWKTDF
                     AAESGRQRHLLQARHADVMLGVILARLQELDTYDDSL"
     gene            1341..>2629
                     /locus_tag="VFP02_11370"
     CDS             1341..>2629
                     /locus_tag="VFP02_11370"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET9543678.1"
                     /translation="MTEESVVGGLKPRSREVTDGYERAGARAMLRAVGMTDEDWAKPQ
                     VGVASSWNEVTPCNLPLDRLAKRSKVGVRDAGGFPLEFTTIAVSDGISMGHEGMRASL
                     VSREVIADSVELMMHAERLDALVTFAGCDKSLPGMLMAAARINMPAVFLYGGSIMPGQ
                     HNGRAIDIVDVFEAVGSFASGGIDEEELSQIERNACPTEGSCAGMFTANTMASVAEAI
                     GMSLPGSASAPAIDSRRDEFAYESGKAVVELLRQNIRPRQIMTKEAFENAIAVVMALG
                     GSTNAVLHLLAIAHEAQVDLELEDFNRVAAKVPHIADTKPHGQYHMSDVDKIGGVPVV
                     MAHLLEAGLLHGDCMTVTGKTIAENLADIAPPAPDGTVVHPLSDAIHDRGGIAVLRGS
                     LAPQGSVVKVAGIDVDRFEGTARVFEGEAAAMEEILRG"
BASE COUNT          405 a          879 c          989 g          356 t
ORIGIN      
        1 agcgagtcgt cgtaggtgtc caactcctgc aggcgggcca ggatcacacc gagcatcacg
       61 tcggcgtgcc gagcctggag gaggtggcgc tggcgcccgg attccgccgc gaagtcggtc
      121 ttccaggcgt attgggcgta ctgatccagg ccctcggcga tgaaaggtgc ctcgtaggtc
      181 aggccggagg gcaggtggta ccagggctgg tgggggtaca cggcgtggag cacgtccagc
      241 tcgggccggt cgccccgccc gatcccggcc gcgaactcgt tgatcgtcgt gggggcgtcg
      301 ggatcggact ggcggatggc gaagtcgacc tggcggtcgt cgcccccgcc gaagagctgg
      361 cgttgccaca ggtcgagggc gtcctgggtc agctcggcga gggggttccc ggtggccagg
      421 gcaccttcgg cgcacaggtg ggccgggcag agctgggtca cggactcctg gacgtggaag
      481 ccgtaggtgc cctgcagcag ggtgaagatg ttgttcgggt gctcttcggc cgtggggagc
      541 acgccgacgg cgtcggggta gcgcccggtc aggatcggag gcacggcttc gggcgtggtg
      601 ggggagacgc cggtggcgtt gcggaaccag gtggcgtcgt cggccaggtg ggccaggttc
      661 gggaactgct ccgggtcgat gcgatcgtcg gcgtcgagga tcgacgccaa cgggaactcg
      721 tcgagcatca ccaggaccac gggcgccggg gccccgacgg cagcggcctc gcccgtggtg
      781 gaggccggat ccaggaccag gccccggacg gggtcggtgg ccaggaacca ggccccgagc
      841 aggaggggga agacgccgag gacctggagc cacaggcggg gcccctccca ccgcgtcacg
      901 gcctgccaca gcaccgcgcc tcccgccacg gcgagaagca cggtgacggg cccgggcagg
      961 ccggcgtcac cgaggaggcg gacgacgacg aggccaccga ggccgccgag caggatccga
     1021 tgcacccggt cgcgcgactg gtcaccgttc cggcctctcc gcaccagtcc gaccagctcc
     1081 tcgatggcga acaggacggc cggcaggccg agcaccagca acagggtcag ggccacgatg
     1141 tcgaggcgac cggcccgtcg gctgacgaag acgtcagcac cctggcgcag cgcggtgagc
     1201 accggttcgg ccacgatgat cccgttcagg gccagcagct cggcgaaccg tcggacgcga
     1261 ccaggcggcg caggagcggc gggcacggcg ggaagttacc ggcgggagcc ccggtgacgg
     1321 tggtcgctac cctcgcggcc gtgaccgaag agagcgttgt cgggggcctg aagccccgtt
     1381 cgcgagaagt gaccgacggc tatgagcggg ccggggcccg ggcgatgctg cgggccgtcg
     1441 ggatgaccga cgaggactgg gccaagcccc aggtcggggt ggcgtcgtcc tggaacgagg
     1501 tgacgccctg caacctgccg ctggaccgcc tggccaagcg gtccaaggtc ggggtgcgcg
     1561 acgccggtgg gttcccgctg gagttcacca ccatcgccgt cagtgacggc atctccatgg
     1621 gccacgaggg catgcgggcc tcgctggtca gccgggaggt catcgccgac tcggtcgagc
     1681 tcatgatgca cgccgagcgc ctggatgcgt tggtgacctt cgccggttgc gacaagtccc
     1741 tgcccggcat gctcatggcc gcggcccgga tcaacatgcc ggcggtcttc ctctacggcg
     1801 gttcgatcat gccgggccag cacaacggcc gggcgatcga catcgtcgac gtgttcgagg
     1861 ccgtgggctc cttcgcctcg gggggcatcg acgaggagga gctgagccag atcgagcgca
     1921 acgcctgccc gaccgagggt tcgtgtgccg gcatgttcac ggccaacacg atggcgtcgg
     1981 tggccgaggc catcggcatg tcgctgcccg gctccgcgtc ggcccccgcc atcgactccc
     2041 ggcgggacga gttcgcctac gagtcgggca aagccgtggt cgagttgctg cgccagaaca
     2101 tccggccccg ccagatcatg accaaggagg cgttcgagaa cgccatcgcc gtcgtcatgg
     2161 cgctgggggg ttcgaccaac gccgtcctcc acctcctggc gatcgcccac gaggcccagg
     2221 tcgacctcga gctggaggac ttcaaccggg tggcggccaa ggtcccccac atcgccgaca
     2281 cgaagcccca tgggcagtac cacatgtccg acgtggacaa gatcggcggg gtgccggtcg
     2341 tcatggccca cctcctcgag gccggcctgc tccacggcga ctgcatgacc gtcacgggca
     2401 agaccatcgc cgagaacctg gctgacatcg ccccgcccgc gccggatggc accgtcgtgc
     2461 acccgctgag tgacgccatc cacgaccgcg gcggcatcgc cgtgctccgg ggctcgctgg
     2521 cgccgcaggg ctccgtggtc aaggtcgccg gcatcgacgt cgaccgcttc gagggcaccg
     2581 cccgcgtctt cgagggtgag gccgcggcca tggaggagat cctccgggg
//
LOCUS       DASTFV010000622         5019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2382141, whole genome shotgun sequence.
ACCESSION   DASTFV010000622 DASTFV010000000
VERSION     DASTFV010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2382141"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..3558
                     /gene="metH"
                     /locus_tag="VFP02_11375"
     CDS             52..3558
                     /gene="metH"
                     /locus_tag="VFP02_11375"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET9543679.1"
                     /translation="MAEFLDLVDERVVIYDGATGTFLQGLDLTADDFGGPELEGCNEF
                     LVVTRPDAIRAFHAAYLEVGCDVVETNTFGAFAIPLAEYDLADRTHELNVTAARLARE
                     VVADFATPDHPRFVAGSVGPGTKMPSLGHISFAELRDAYQVQMDGLLEGGVDLLVIET
                     QYDLLGLKAAMIAARRAMAAQGREVPLQVQVTIELTGRMLPGTEIGGALAAIDPLRPD
                     VIGLNCATGPTEMGEHLRHLSQHARMPISCLPNAGLPQVIDGKMAYDLTPDQLAEHHK
                     RFITELGVQVVGGCCGTGPEHIRRVVEVCRDLSPAARTPVHEPGATSIYSHVPFEQDT
                     SFLIIGERTNANGSKKFREAMLDGDWDTCTLMGREQLSEGAHVLDLCVDYVGRDGTLD
                     MDELASRFGTQVAAPLVLDSTEPQVMEAGLQHIGGRSLLNSANLEDGEAPGSRLDRVL
                     TLCKEYGAAAICLLIDEEGQARDVEWKMRVAHRIHDIAVERYDLEPSDLVFDALTFPL
                     STGGEDLRGDGLATIEAIRRIKAELPGVHTTLGVSNVSFGLKPAARHVLNSVFLHECV
                     EAGLDSAIVHAARIMPLNRIPDEQRQVALDLVHDRRGTTGGDGGDPEYDPLHRLLALF
                     EDVEATEVVKEDRSGWPVEQRLSQRIIDGERDGLEADLDEALANGTPALLIINDTLLA
                     GMKVVGELFASGEMQLPFVLQSAETMKAAVKFLEPHMEKADDAGKGRLVLATVKGDVH
                     DIGKNLVDIILTNNGYEVHNLGIKISITEMIEKAKAVQADAIGMSGLLVKSTLIMRDN
                     LEELNAQGLAEIPVMLGGAALTRSYVERDLREIYEGRLFYGKDAFEGLHVMDRIGESR
                     RRGEELGDWGLVPSESKVPSRIKEMFASGDEPPVDLPDRSPEVAADNPVFAPPFLGTR
                     VVKGIAIDEVAGYINETALFRNQWQFRPHKGEDDAAFKERIRPTRREQLGTAKADDLL
                     VPQVAYGYFAANKDGDDLVIWKDETRTGELARFSFPRQSADPFLCISDFFRSVDSGEA
                     DYAAFHLVTMGAPVSERAAELFAADRYQDYLLLHGLGVEMAEALAEYWHRRIREEWGF
                     AHEDGPDLNGLFRQKYRGGRYSFGYPACPDLADNEKAWEILDGARLGIEVSEETGFQF
                     QPEQTTCALICHHPRAKYFVAR"
     gene            complement(3972..4889)
                     /locus_tag="VFP02_11380"
     CDS             complement(3972..4889)
                     /locus_tag="VFP02_11380"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HET9543680.1"
                     /translation="MPSPAQIAVAPEGQRPWLAEAVRAGGATVVDAAAAEGLVWASTG
                     GADDLAALLVGCPGIRWVQLPWAGIEPFVAVLDDRHVWTCGKGVYAAPVAEHALTLGL
                     AGLRGLDRYARTTRWSPPRGENLVGAAVTILGGGGITEELLRLLAPFGCAVTVVRRHP
                     APMAGAVEVVGPDELRAALPGRRLVVLALALTPATVGTIGAEELAAMDDDAWLVNVAR
                     GGHVVTDDLVAALRDGTIGGAALDVTDPEPLPDGHPLWSQPNCLITPHVGNTPEMARP
                     LLSARIADNVRRFGAGEPLVGRVDPALGY"
BASE COUNT          806 a         1878 c         1671 g          664 t
ORIGIN      
        1 gaacgcaaat ccccgaaggg ggccgagacc gcccctcggt agcctctggg gatggccgag
       61 ttcctcgatc tcgtcgacga gcgcgtcgtc atctacgacg gcgccacggg gacgttcctg
      121 caaggcctcg acctgaccgc cgacgacttc ggcggccccg agctcgaggg ctgcaacgag
      181 ttcctcgtcg tcacccggcc cgacgccatc cgggcgttcc acgccgccta cctcgaggtg
      241 ggctgtgacg tcgtcgaaac caacaccttc ggcgccttcg ccatcccgct cgccgagtac
      301 gacctggccg accgtacgca cgagctgaac gtcaccgccg cccgcctcgc ccgcgaggtc
      361 gtggccgact tcgccacgcc ggaccacccc cggttcgtcg ccgggtcggt cgggcccggg
      421 accaagatgc cctccctcgg ccacatcagc ttcgccgagc tccgggacgc ctaccaggtc
      481 cagatggacg ggctgctcga gggcggggtc gacctcctcg tcatcgagac ccagtacgac
      541 ctgctcggcc tcaaggcggc catgatcgcc gcccgccggg ccatggccgc ccagggccgc
      601 gaggtcccgc tgcaggtcca ggtcaccatc gagctgaccg gccgcatgct gccgggcacc
      661 gagatcggcg gcgccttggc cgccatcgac ccgctgcgcc ccgacgtcat cgggttgaac
      721 tgcgccaccg ggccgaccga gatgggtgag cacctgcgcc acctgtccca acacgcccgc
      781 atgccgatct cctgcctgcc caatgcaggc ctgccgcagg tgatcgacgg caagatggcc
      841 tatgacctga cgccggacca gctggccgag caccacaagc gcttcatcac cgagctgggc
      901 gtccaggtcg tcggcggctg ctgcggaacc gggcccgagc acatccgacg ggtcgtcgag
      961 gtgtgccggg acctgtcgcc ggccgcccgc acgccggtgc acgagccggg cgccacgtcg
     1021 atctacagcc acgtgccctt cgagcaggac acgtcgttcc tcatcatcgg cgagcggacc
     1081 aacgccaacg gctccaagaa gttccgcgag gccatgctcg acggcgactg ggacacgtgc
     1141 acgctgatgg gccgcgagca gctcagcgag ggcgcccacg tcctcgacct ctgcgtcgac
     1201 tacgtcggcc gcgacggcac cttggacatg gatgagctgg cctcccggtt cgggacccag
     1261 gtcgcggctc ccctcgtgct cgactccacc gagccgcagg tcatggaggc cggcctccag
     1321 cacatcggcg gccgctccct gctcaactcg gccaacctcg aagacggcga ggcaccgggc
     1381 tcccgcctgg accgggtcct caccctctgc aaggagtacg gcgccgccgc catctgcctg
     1441 ctcatcgacg aagagggcca ggcccgcgac gtggagtgga agatgcgggt ggcccaccgc
     1501 atccacgaca tcgccgtcga gcgctacgac ctcgagccct ccgacctcgt cttcgacgcc
     1561 ttgaccttcc cgctctcgac cggcggcgag gacctccgcg gcgacggcct ggccaccatc
     1621 gaggccatcc gccggatcaa ggccgagctg cccggggtcc acacgaccct cggcgtgtcc
     1681 aacgtgtcct tcggcctcaa gcccgcggcc cgccacgtcc tcaactcggt gttcctgcac
     1741 gagtgcgtcg aggccgggct cgactcggcc atcgtccacg cggcgcggat catgcccctc
     1801 aaccgcatac cggacgagca gcgccaggtc gcgctcgacc tggtccacga ccgccgggga
     1861 accaccggtg gcgacggcgg cgaccccgag tacgacccgc tccaccgcct gctggccctg
     1921 ttcgaggacg tcgaggccac cgaggtcgtc aaggaggacc gctcgggatg gcccgtcgag
     1981 cagcggttgt cccagcgcat catcgacggt gagcgcgacg ggctcgaggc cgacctcgac
     2041 gaggcgctcg ccaacggcac gccggccctg ctgatcatca acgacaccct gctggcgggc
     2101 atgaaggtcg tgggcgagct cttcgcctcc ggtgagatgc agctgccctt cgtgctccag
     2161 tcggcggaga ccatgaaggc cgcggtcaag ttcctcgagc cccacatgga gaaggccgac
     2221 gacgccggca agggccgcct cgtgctggcc acggtcaagg gcgacgtcca cgacatcggc
     2281 aagaacctcg tcgacatcat cctcacgaac aacggctacg aggtccacaa cctcggcatc
     2341 aagatctcga tcaccgagat gatcgagaag gccaaggccg tgcaggccga cgccatcggg
     2401 atgagcggcc tgctggtcaa gagcacgctg atcatgcggg acaacctcga ggagctgaac
     2461 gcccagggtc tggccgagat cccggtgatg ctgggtggcg ccgccctcac ccgctcctac
     2521 gtggaacggg acctgaggga gatctacgag ggtcgcctct tctacggcaa ggacgccttc
     2581 gaggggctcc acgtcatgga tcgcatcggt gagtcccgcc gccgcggcga ggagctcggt
     2641 gactggggcc tggtcccctc ggagtccaag gtcccctcgc ggatcaagga gatgttcgcg
     2701 tccggcgacg agccaccggt cgacctccct gaccgttccc ccgaggtggc cgccgacaac
     2761 ccggtcttcg cgcccccgtt cctcggcacc cgggtggtca agggcatcgc catcgatgaa
     2821 gtagccggct acatcaacga gacggcgctc ttccggaacc agtggcagtt ccgaccgcac
     2881 aagggcgagg acgacgccgc gttcaaggag cgcatccgcc ccacccggcg cgagcagctc
     2941 gggacagcga aggcagacga cctgctcgtg ccgcaggtgg cctacgggta cttcgccgcc
     3001 aacaaggatg gcgacgacct cgtcatctgg aaggacgaga cgcgcacggg cgagctggcc
     3061 cgcttcagct tcccccgcca gagcgccgac ccgttcctgt gcatcagcga cttcttccgc
     3121 tcggtcgact ccggcgaggc cgactacgcc gccttccacc tggtcacgat gggggccccg
     3181 gtcagcgagc gggccgccga gctcttcgcg gccgaccgct accaggacta cctgctgctc
     3241 cacggcctcg gggtcgagat ggccgaggcc ctggccgagt actggcaccg gcgcatccga
     3301 gaggagtggg gcttcgccca cgaggacggt cctgacctga acgggctgtt ccgccagaag
     3361 taccggggag ggcgctattc gttcggctac ccggcgtgcc cggacctggc cgacaacgaa
     3421 aaggcctggg agatcctcga cggcgcccgt ctgggcatcg aggtcagcga ggaaacgggc
     3481 ttccagttcc agcccgaaca gaccacctgc gccctcatct gccaccaccc ccgggccaag
     3541 tacttcgtcg cccggtagcc gccggcgcgc cccacccgag gggagaggtc tccccttgac
     3601 agggcctggg cacgggtgga cagtcagagg tgacaccgcc agctcaccga ggaggcccag
     3661 aaatggccac ggcgcacgaa gcaggcgatc cgcccgacca ttgacccgct gaggcccagg
     3721 cctcagcgaa gcccgccagt cgatcgggac cgaccgttcc cgacccacca gcgagccttc
     3781 ggcagcgcga tgaaccctct ccgcacggcg accgtgtgat ccgcccgctc ccgccccgcc
     3841 caccggtcgg gcggtccggc gccggcgagg ggtgatccgc ctccgggttc gaccgaacgc
     3901 atggcgcgag cgtccggcac cggtcgacga ccagagccac cgacccgccc tccggggcgg
     3961 gtcggggcgc gtcagtagcc cagcgccggg tccacccggc cgaccaaggg ctcccctgcc
     4021 ccgaaccgcc ggacgttgtc ggcgatgcgg gccgagagga gcggtcgggc catctcgggc
     4081 gtgttgccca catgcggggt gatcaggcag ttgggctgcg accacaacgg gtgaccgtcg
     4141 ggcaggggct cgggatcggt cacgtccaag gcggcgcccc cgatggtgcc gtcgcgcagg
     4201 gcggcaacca ggtcgtcggt gacgacgtgg ccgccgcggg ccacgttcac caaccaggcg
     4261 tcgtcgtcca tggcggccag ctcctcggcg ccgatggtcc ccacggtcgc cggcgtgagg
     4321 gccagggcca agacgaccag gcggcgcccc ggcagcgcgg ccctcagctc gtcgggcccg
     4381 accacctcca ccgcccccgc catcggcgcc gggtggcgcc gcaccaccgt cacggcgcag
     4441 ccgaaggggg cgagcagtct gagcagttcc tccgtgatgc cgccgccgcc gaggatcgtg
     4501 accgccgcgc cgacgaggtt ctcgccccgg ggcggtgacc acctggttgt ccgcgcgtac
     4561 cggtcgagcc cccgcaggcc cgccaggccc agggtcagcg cgtgctcggc gaccggtgcg
     4621 gcgtagacgc ccttcccgca cgtccagacg tggcgatcgt cgagcacggc gacgaagggt
     4681 tcgatgccgg cccacggcaa ctggacccaa cgaatgcccg ggcacccgac gaggagggcg
     4741 gccaggtcat cggcaccacc cgtggaggcc cagaccagac cctcggccgc agcggcgtcg
     4801 acgaccgtgg ccccgcccgc tctgacggcc tcagccagcc acgggcgctg gccttccggc
     4861 gccacggcga tctgggcggg ggacggcatc gtcggcaacg taatcccgga aatggcaagg
     4921 ttgggcatgg cgctcctggt cggtgtcccc cgtgagatca agaacaacga gaaccgggtg
     4981 gcgatcaccc cggacggcgt gcgggagttg gcccagcac
//
LOCUS       DASTFV010000623         3070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2382767, whole genome shotgun sequence.
ACCESSION   DASTFV010000623 DASTFV010000000
VERSION     DASTFV010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2382767"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VFP02_11385"
     CDS             complement(<1..448)
                     /locus_tag="VFP02_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9543681.1"
                     /translation="MAKIDKAVDRLVDDRPGAELLLPRYDDPAHPVAEGIWRSGGTTA
                     AYLIGADAGRVVVNTGMGFEAPHHRRVFDAVHPGPTPYIVTTQAHVDHVGGVGLFREP
                     DTRYVAQAANPACQADDLRIQRFRFSTAMIWFGQLPEIIGRLAEESP"
     gene            complement(448..1704)
                     /locus_tag="VFP02_11390"
     CDS             complement(448..1704)
                     /locus_tag="VFP02_11390"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9543682.1"
                     /translation="MSPEPIRIQDLAEPVLSDHQRGALAFGEEVATELSVSAVLTAAV
                     DRTGLDDFGPDDFRDRLGRWLGEVDRDTDRTGLGRLILFNDCVRYAANRLRIHDLLAR
                     HPEIHDEATVAPIIVVGLPRSGTTHLVNLIAADPRLRSLPLWESYEPVPTPGEPATRD
                     AGDPRHQRCAGAWEAMQQTVPFLAAMHPMAPDHVHEELELQLPDFTSYELEWVARVPE
                     WRDHYLATDQTPHYEYLRTVLKILQWYSPGERWVLKSPQHLEQLGPLLATFPDATVVV
                     THRDPVSVIQSAATMVTYGARMSYRTTEPEWYLSYWADRIERLLSASVRDRHLVPADR
                     SVDVLFHEFMADDVATVERIYETADLTMTAEARTAIDRYRAGHERGRDGQVVYDVRRD
                     FGAEPDALRAPFAFYFHRFAVQEEVR"
     gene            complement(1701..2450)
                     /locus_tag="VFP02_11395"
     CDS             complement(1701..2450)
                     /locus_tag="VFP02_11395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9543683.1"
                     /translation="MTSVAFDFAGAHVLVTGGSNGIGLGVARAFADAGAKVTITGTRE
                     DAGAYDHDLARFAYRRCRMTDADEVQALADGLDALDVLVNNAGQNLPGGRSEWDDGVF
                     EEVLAVNLVAPFRLAQACHDRLAASDVDGGSSVVNVGSMASFFGMEIVPGYGAAKGGV
                     VQTTKTLAVSWARDGIRVNAVAPGVVETNMTAPMLPLDEITAPLLARTPMGRFATPDD
                     VAGAVLFLASPAARYVTGHTLVVDGGFSVFG"
     gene            2488..3024
                     /locus_tag="VFP02_11400"
     CDS             2488..3024
                     /locus_tag="VFP02_11400"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9543684.1"
                     /translation="MTADPTTVFPGAIRQNGYVVRDLDAAIESWLDLGVGPWITLGPM
                     EQGMVFRGEPTALTITLAFANSGDLQIELIHQTGDAPSAYKEFLDSGREGFHHLAWWA
                     EDVAAVEAAVDAAGWPVIFKGDGTSVAAFFYCEAPEVAATCLEVMELNEMTQGLADHV
                     RQAADDWDGTTEPVRKLF"
BASE COUNT          435 a         1035 c         1154 g          446 t
ORIGIN      
        1 cggggctctc ctcggccagg cggccgatga tctcgggcag ctggccgaac cagatcatgg
       61 cggtggagaa acggaagcgc tggatgcgca ggtcgtcggc ctggcaggcc gggttggcgg
      121 cctgggccac gtaccgggtg tccggctcgc ggaacagccc gaccccgccg acgtgatcga
      181 cgtgggcctg ggtggtgacg atgtacggcg tcgggcccgg gtggacggcg tcgaacacgc
      241 gcctgtggtg cggggcttcg aagcccatac cggtgttgac gacgacccgg ccggcgtcgg
      301 cgccgatcag gtaggcggcc gtggtcccgc ccgagcgcca gatgccttcg gccacggggt
      361 gggccgggtc gtcgtagcgg gggaggagca gctcggcccc gggccggtcg tcgacgagcc
      421 ggtccaccgc cttgtcgatc ttggccatca gcgcacttcc tcctgcacag cgaagcggtg
      481 gaagtagaag gcgaacgggg cccgcagcgc atcgggctcg gcgccgaagt cccggcgcac
      541 gtcgtatacg acctggccgt ccctgccccg ctcgtgaccg gcccggtacc ggtcgatcgc
      601 ggtccgggcc tcggccgtca tggtcaggtc ggcggtctcg tagatgcgct cgacggtggc
      661 gacgtcgtcg gccatgaact cgtggaagag gacgtcgacc gaccgatcgg cggggaccag
      721 gtgtcggtcc cgcaccgagg ccgacagcag gcgctcgatc cggtcggccc agtaggacag
      781 gtaccactcg ggctccgtcg tgcgatagct catgcgggcc ccgtaggtca ccatcgtggc
      841 cgccgactgg atgaccgaca ccggatcccg gtgggtgacg acgacggtgg catcggggaa
      901 ggtggcgagt agagggccga gctgctccag gtgctgaggt gacttcagga cccagcgctc
      961 gccgggtgag taccactgca ggatcttcag cacggtgcgc aggtactcgt agtgcggggt
     1021 ctggtcggtg gccaggtagt ggtcccgcca ttcggggacc cgggccaccc actccagctc
     1081 gtagctggtg aagtccggga gttgcagctc cagctcttcg tggacgtggt cgggcgccat
     1141 cgggtgcatt gcggccagga aggggacggt ctgctgcatg gcctcccagg ccccggcgca
     1201 gcgttggtgg cgcgggtcgc ccgcatcccg cgtggcgggc tcgccgggtg tgggcacggg
     1261 ctcgtagctc tcccacagcg ggagcgagcg caggcggggg tcggcggcga tgaggttgac
     1321 caggtgcgtg gtgccggagc gagggagccc caccacgatg atcggggcca cggtcgcctc
     1381 gtcgtggatc tcggggtgtc gggccagcag gtcgtggatg cgcaggcggt tggcggcgta
     1441 gcggacgcag tcgttgaaga ggatcaggcg gccgaggccg gtgcggtccg tgtcccggtc
     1501 gacctcgccc agccagcgtc cgagccggtc ccggaagtca tcggggccga agtcatcgag
     1561 cccggtgcgg tcgacggcgg cggtgagcac ggcactcacg ctcagctcgg tggccacctc
     1621 ctcgccgaag gccagcgccc cccgctggtg atcgctgagg accggctcgg ccaggtcctg
     1681 gatgcggatc ggctcggggc tcacccgaag accgagaagc cgccgtcgac cacgagggtg
     1741 tggccggtga cgtagcgggc ggccgggctg gcgaggaaga ggacggctcc ggcgacgtcg
     1801 tccggcgtgg cgaagcggcc catcggcgtg cgggccagga ggggggcggt gatctcgtcg
     1861 aggggcagca tcggggcggt catgttcgtc tcgaccacgc cgggggcgac ggcgttgacc
     1921 cgaatgccgt cccgggccca gctcacggcc agggtcttgg tcgtctgcac gaccccgccc
     1981 ttggccgcgc cgtagccggg cacgatctcc atcccgaaga agctggccat ggagccgacg
     2041 ttgaccacgc tgctcccgcc gtcgacgtcg ctggcggcca ggcggtcgtg acaggcctgg
     2101 gccaggcgga agggggcgac caggttgacc gccagcacct cctcgaagac cccgtcgtcc
     2161 cactcgctgc gcccgccggg caggttctgg ccggcgttgt tgaccaacac gtcgagggcg
     2221 tcgagcccgt cggccagtgc ctgcacctcg tcggcgtcgg tcatacgaca gcggcggtag
     2281 gcgaaccggg cgaggtcgtg gtcgtaggcg ccggcatcct cgcgggtgcc ggtgatggtg
     2341 accttggccc cggcgtcggc gaaggcgcgg gccacaccga ggccgatccc gttggagccc
     2401 ccggtcacca gcacgtgcgc cccggcgaag tcgaacgcga cgctcgtcat gcccgtcctg
     2461 ccacgaccgc ctaccgtagg acgtgccgtg accgccgatc ccacgaccgt cttcccgggg
     2521 gccatccggc agaacgggta cgtcgtccgc gacctcgacg ccgccatcga gtcatggctg
     2581 gacctcggtg tgggcccgtg gatcaccctc ggcccgatgg agcagggcat ggtgttccgg
     2641 ggcgagccga cggcgctcac gatcacgctg gccttcgcca actccgggga cctgcagatc
     2701 gagctcatcc accagaccgg tgacgccccg agcgcgtaca aggagttcct ggactccgga
     2761 cgtgagggct tccaccacct ggcctggtgg gccgaggacg tcgcggccgt cgaggccgcc
     2821 gtcgacgcgg ccggctggcc cgtcatcttc aaaggtgacg gcaccagcgt cgccgcgttc
     2881 ttctactgcg aagcccccga ggtcgccgcc acctgcctcg aggtcatgga gctgaacgag
     2941 atgacccagg gcctcgccga ccacgtccgc caggccgcag acgactggga cggcaccacc
     3001 gagccggttc gcaagctgtt ctgaccgccg tcgtcaccag acgagctccc actgcctgga
     3061 ctcggccgag
//
LOCUS       DASTFV010000624         6335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2383236, whole genome shotgun sequence.
ACCESSION   DASTFV010000624 DASTFV010000000
VERSION     DASTFV010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2383236"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..511)
                     /locus_tag="VFP02_11405"
     CDS             complement(197..511)
                     /locus_tag="VFP02_11405"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET9543685.1"
                     /translation="MTAWATILLAGLATYALRALPARWAGRRPLPEAWQRATVALGPA
                     AFAALAAPAAVLGSGSASPAVARLGAVAVALPLARRTRSTTVVLLAGMSTLWLVTIVA
                     GG"
     gene            complement(508..1221)
                     /locus_tag="VFP02_11410"
     CDS             complement(508..1221)
                     /locus_tag="VFP02_11410"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET9543686.1"
                     /translation="MTTPMTAATTDGPRATTAGARAMAPLLVSVAPLGLVVGLKAAAA
                     DLPAAAGWATSFTIYGASAQLAAIDLLDRGVAPALVVLTVVVVNARLALYSAALGRSW
                     STASGPWQALAAYLLVDPSYATGIDGYARPLTTRERHTFYLAAAATLWVGWQLLTLAG
                     MTLGTHVPAGLDLGFAAPLCLLAIAARQVDDRSSAVTAVVAAVVAVLGHGLPMATGLP
                     LAIVAGILAGATRERGTRP"
     gene            complement(1347..1916)
                     /locus_tag="VFP02_11415"
     CDS             complement(1347..1916)
                     /locus_tag="VFP02_11415"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9543687.1"
                     /translation="MTITESDPAVRNGVDTATLFATIDAVRAQPAAAQFQFRATNRWV
                     SGTHSRGYVQGFFGVGAEDMSREEPFVLNADHPTVLVGSDEGPTPVEYVLHALAACLT
                     AGIANISAARKVTVHRVESTIEGDIDLQGILGLDPDVRNGFRAVRVRFAIDADATDEV
                     IAEIVEQASARSAVLDIITNGVPVDVTVG"
     gene            complement(1976..3160)
                     /locus_tag="VFP02_11420"
     CDS             complement(1976..3160)
                     /locus_tag="VFP02_11420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9543688.1"
                     /translation="MNATVTPDRDVVVVGARCAGAATAMLLARQGHRVTLVDRATFPS
                     DTISTHAVARGGVVQLARWGLLDAVLASGAPAIRRVRFYVGGEVIDRAIKERSGVDLL
                     VAPRRHVLDHLLVEAAREAGAEVRTGVTMTGVTRAGARVTGVEGHDAEGDAVEISARF
                     VVGADGLRSRVARDVGAPIVDQRPPGGATHYAYFAGPAWDGFEFHIADRVMAGVFPTH
                     DGEANVWACTPAERTDELREDRSAGLLRLLAMANPDLAERIDAAEQTSPVRGAVSLPN
                     HIRRAWGPGWALVGDAAMHRDPVTGHGITDAFRDGELMARTLDAVIRGELAEGVAGRS
                     YEALRHDLVREQFDATVRMSQYPPVEEFVEQQFRSTAAIEAEARFLADLPSMTPPLAA
                     VA"
     gene            3217..5763
                     /locus_tag="VFP02_11425"
     CDS             3217..5763
                     /locus_tag="VFP02_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET9543689.1"
                     /translation="METAQEGKRDVQATTTFGDVELDRAGHELRRAGEVVPVEPQVFD
                     VLAYLVEHRERLVLKTELLDEVWGDRFVSESALTSRIKAARRAVGDNGRDQRVIRTVH
                     GRGYRFVAEVDDGPAAKSANSASDLHAVIEDLREGRGSAVLVVAPPGRARRELLDELY
                     ADAGAAGILAARGRIVQLGVLAGVVDALDEIGQRRPALVERVPDGCRSELATVFAGGP
                     PSTIGRLQLAARELVLAAAEDGGILLVLHDLEMADRATLVQVEQWAQLARRAPIGLVV
                     AHRDPGVLAGLTVHEPDRLRPRPTDRYVPELPEEVTTVLTRIALVGPAIDPLTFRAVS
                     GVTPEQADRVLELAVGAGLLERDGDTYRFASADAATALVDRLGPHRRAELHHEVAEAL
                     AAAGAPAPVVAEHYLAAGEASAAVPFVLEAARGASSVHAHSDVLALTASVLDHADGDA
                     RVELLALRAMALVATGDTSAPRYLREALALAGPEWVPMLRLGLAQASILSSDVDGAAD
                     ALEGLELDGGTDDGAILLLRGMVAFFRGDIDAAELAARDARDLALAPGAPGALLDVIA
                     LEGMIAHNRGEWFDRIRRELRATQDDPVLATKVFDGQLCVTEYLLYGPLPYDEVVALA
                     RGLQATAEQSGARRAEAFALCLSGEALFLAGHLDEARVDLERSMAMHQDLHADAGTAL
                     CLQRLAEVELANGDRETAARHARQALSLARWSPLARHLIQRIYGTLIAIAPDVDAAVA
                     LVAEAENAADPAARCQYCDIMLAVPATAALAEAGRLDEARSSLERAQRAAGLWHGTAW
                     QGAVAEAEAVLARAEGRGDEAEALLARAAGLFDEAGQPLDAARCREAAIG"
     gene            complement(5777..>6335)
                     /locus_tag="VFP02_11430"
     CDS             complement(5777..>6335)
                     /locus_tag="VFP02_11430"
                     /inference="COORDINATES: protein motif:HMM:NF001368.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET9543690.1"
                     /translation="SSLLSERIVFLGQEVDDQIANLLIAQILHLEAQDPDKDINLYIN
                     SPGGLAYGGMAIYDTMQHVRCDVATICVGMGMSAAAMILCGGAEGKRFALPSAKLMIH
                     QGSAGARGAPRDMEIHLREVLATTHRMTEILSHHSGRSMEEVEHDLDRDYFMTAEEAK
                     AWGLIDDILVPRRGVSAPREVAVAS"
BASE COUNT          819 a         2213 c         2451 g          852 t
ORIGIN      
        1 cgcacggccg gcctggtcgc ccacatcgct gaggagaacc gtgaccccat cggcctcccc
       61 ctctggctcg aggtcgaagg ccgcagctga tcagttggcg gcgtagagcg gccaggtctc
      121 gggcacgccc ttcagctggt ggatgccgcg cccgtcgaag gtcaggccgg agccaacggc
      181 cggcgtgcgc ccaggctcaa ccgccggcca cgatcgtgac cagccacaac gtggacatgc
      241 ccgccagcag cacgacggtg gtggaccgtg tcctgcgggc gagggggagg gccaccgcca
      301 cggcccccag ccgggccacc gccgggctgg cggatcccga tcccaggacc gccgccggtg
      361 cggccagcgc cgcgaaggcg gccgggccca gggcgaccgt ggcccgttgc caggcctcgg
      421 gcaatggtcg gcgaccggcc cagcgggccg gaagggcacg cagggcgtac gtggccagcc
      481 ccgcgaggag gatggtggcc caggccgtca tggtcgggtc cctcgctcac gggtcgcacc
      541 cgcgaggatc ccggcgacga tggccagggg aaggcccgtg gccatcggca gcccgtggcc
      601 gaggacggcg accacggccg cgacaacggc ggtcacggca ctggatcggt cgtcgacctg
      661 gcgggccgcg atggccagga ggcagagggg ggcagcgaag ccgaggtcga ggccggcggg
      721 gacatgcgta ccgagcgtca tcccggcgag cgtgaggagc tgccacccga cccagagcgt
      781 cgccgccgcg gccaggtaga acgtgtgtcg ctcccgcgtc gtcagcggtc gggcgtagcc
      841 gtcgatgccc gtggcgtagg acgggtccac gaggaggtag gcggcgaggg cctgccacgg
      901 cccgctcgcc gtgctccacg agcggccgag cgcggcgctg tagagcgcca gtcgggcgtt
      961 gacgacgacc acggtcagga cgacgagcgc cggggccacg ccgcggtcga gcaggtcgat
     1021 ggccgccagc tgggcgctgg cgccgtagat cgtgaagctg gtggcccatc ccgccgcggc
     1081 ggggaggtcg gccgcggccg ccttcaaccc gacgacgagc ccgaggggag ccacgctcac
     1141 gaggagggga gccattgctc gggctccagc ggtggtcgct cggggtccgt cggtcgtggc
     1201 cgcggtcatc ggtgtggtca tgagggcatc gtcggggatt cggagctgtc ggtcttggag
     1261 gggatcgggt gaagtcgtgg agatcggccg gtgacgcgcc cgagggggcg cggccgtcgg
     1321 ccacgccccc tcaggaccca tcgggatcaa ccgacggtga cgtcgacggg gaccccgttg
     1381 gtgatgatgt cgaggacggc ggagcgggcc gaggcctgct ccacgatctc ggcgatgacc
     1441 tcgtcggtcg catcggcgtc gatcgcgaac cgcacgcgga cggcccggaa gccgttgcgc
     1501 acgtcggggt ccaggccgag gatgccctgc aggtcgatgt cgccctcgat ggtcgactcg
     1561 acccggtgca cggtcacctt ccgggccgcc gagatgttgg cgatgccggc ggtgaggcac
     1621 gcggccaggg cgtggagcac gtactccacc ggcgtcgggc cttcgtcgga gccgaccagc
     1681 acggtcgggt gatcggcgtt gaggacgaag ggttcctcgc gggacatgtc ctcggcgccg
     1741 acgccgaaga agccctggac gtagccccgg ctgtgggtgc ccgagaccca gcggttggtc
     1801 gcccggaact ggaactgggc ggccgccggc tgggcccgca cggcgtcgat ggtcgcgaac
     1861 agggtcgcgg tgtcgacgcc gttgcgcaca gcgggatccg actcggtgat ggtcatgggt
     1921 actgctcctt tggtgttggt gttggtgttg gttggacggt ggcgggatcg gtgcctcagg
     1981 cgacggccgc gagcggcggc gtcatggacg ggaggtcggc caggaaacgg gcctcggcct
     2041 cgatggccgc ggtggagcgg aactgctgct cgacgaactc ctcgaccggc gggtactggg
     2101 acatgcggac ggtggcgtcg aattgctcgc gcaccaggtc gtggcgcagg gcctcgtagc
     2161 tacggccggc gacgccttcg gccagctcgc cccggatcac ggcgtcgagg gttcgggcca
     2221 tcagctcgcc gtctcggaag gcgtcggtga tcccgtggcc cgtcaccgga tcgcggtgca
     2281 tggcggcatc cccgaccagg gcccagcccg ggccccacgc gcggcggatg tggttgggca
     2341 ggctgaccgc gccgcggacg gggctggtct gttccgccgc atcgatgcgc tcggccaggt
     2401 cggggttcgc catggccagg aggcgcagca gcccggcgga acgatcctcc cgcagctcgt
     2461 cggtccgttc cgccggcgtg cacgcccaca cgttggcttc gccgtcgtgg gtcgggaaca
     2521 cccccgccat cactcggtcg gcgatgtgga actcgaagcc gtcccacgcc ggcccggcga
     2581 agtaggcgta gtgcgtggcc ccacccggcg ggcgttggtc gacgatcggc gcgccgacgt
     2641 cgcgggccac acgagagcgg aggccgtcgg ccccgacgac gaatcgggcc gagatctcga
     2701 cggcgtcgcc ctcggcgtcg tgaccctcca cgccggtcac ccgggcgccg gcccgggtga
     2761 cgccggtcat ggtcacgccc gtgcgcacct ccgccccggc ctctctggcg gcctcgacca
     2821 gaaggtggtc gaggacgtgg cggcggggcg cgaccaggag gtcgacgcct gagcgttcct
     2881 tgatcgcccg gtcgatcacc tcgccgccga cgtagaagcg gacccggcgg atcgccgggg
     2941 cccccgaggc caggacggcg tcgaggaggc cccagcgggc cagttgcacc acaccgcccc
     3001 gggcgacggc gtgggtggag atcgtgtcgc tggggaaggt ggcccggtcg accagcgtga
     3061 cccggtggcc ttgccgggcg agcagcatgg ccgtggccgc accggcgcac cgggcgccga
     3121 cgacgacgac gtcgcgatca ggggtgacgg tggcgttcat gtctgccaga gtgcgggacg
     3181 gtcgcccgcc ggtcttggag acgatcgggg gatcttgtgg agacggcgca ggaggggaag
     3241 cgggacgtgc aggccaccac gaccttcggc gatgtggagc tggaccgggc cggtcacgag
     3301 ctccgccggg cgggtgaggt cgtacccgtc gagccccagg tgttcgacgt cctcgcctac
     3361 ctcgtcgagc accgcgagcg gctggtgctg aagacggagc tgctcgacga ggtctggggg
     3421 gaccgtttcg tctccgagtc ggccttgacc agccgcatca aggccgcccg ccgggccgtg
     3481 ggggacaacg gacgggacca gcgggtcatc cgcacggtgc acggccgggg gtaccgcttc
     3541 gtcgccgaag tggacgatgg tccggccgcg aagtcagcga actcggcgtc cgacctccac
     3601 gccgtgatcg aggacctccg ggagggccgc ggcagtgcgg tgctggtggt cgcgccgccg
     3661 ggccgggcgc ggcgggagct gctggacgag ctctacgccg acgccggggc ggccggcatc
     3721 ctggccgccc ggggccggat tgtgcagctc ggcgtcctgg cgggggtggt cgacgccctc
     3781 gacgagatcg gccagcgccg cccggccctc gtggagcggg tgcccgacgg ctgccgctcg
     3841 gagctggcga cggtgttcgc cggaggaccg ccgtcgacga tcgggcggct ccagctcgca
     3901 gcccgggagc tcgtgttggc cgcggcggag gacgggggga tcctgttggt gctccacgac
     3961 ctcgagatgg ccgaccgggc cacgctcgtg caggtcgagc agtgggccca attggcccgt
     4021 cgggccccga tcggcctggt cgtcgcccac cgggatccgg gggtgctggc cggcctgacc
     4081 gtccacgagc cggaccggct gcgtccgcgg cccaccgacc ggtacgtgcc cgagctcccc
     4141 gaggaggtca cgaccgtact gacgcggatt gcgttggtgg ggccggcgat tgaccccctc
     4201 accttccggg cggtgtcggg cgtgaccccg gagcaggctg accgggtcct cgagctcgcc
     4261 gtcggggccg gtctgctcga gcgggacggg gacacgtacc gcttcgcctc ggccgacgcc
     4321 gccaccgcgc tcgtcgaccg cctcggcccc caccgccgcg ccgagctgca ccacgaggtg
     4381 gctgaggcgc tggccgccgc cggcgcgccg gctccggtgg tggctgagca ctacctggcg
     4441 gccggcgagg cgtccgccgc cgtccccttc gtcctggaag ccgcccgcgg ggcctcgagc
     4501 gtccacgccc acagcgacgt gttggccctg accgcatccg tgctcgatca cgccgacggc
     4561 gacgcccggg tggagctgct cgccttacgg gccatggccc tggtcgccac cggcgacacc
     4621 tcggcccccc gttacctccg ggaggccctg gccctggccg gcccggagtg ggtcccgatg
     4681 ctgcgcctcg ggctggccca agcatcgatc ctctccagtg acgtcgacgg ggcggccgat
     4741 gcgctggagg gcctcgagct cgacggcggc accgacgacg gcgccatcct cctcctgcgg
     4801 gggatggtgg cgttcttccg gggcgacatc gacgccgccg agctcgccgc ccgcgacgcc
     4861 cgagatctgg cgctggcgcc cggggcaccc ggcgccctgc tggacgtcat cgccctcgag
     4921 ggcatgatcg cccacaaccg tggggagtgg ttcgaccgca tccggcgcga gctgcgggcg
     4981 acccaggacg atccggtgtt ggccaccaag gtgttcgacg gccagctctg cgtgaccgag
     5041 tacctgctct acgggcccct gccgtacgac gaggtcgtcg cgctggctcg tggcctgcag
     5101 gccaccgccg agcagagcgg ggcccggcgg gcggaggcct tcgccctgtg cctgtccggt
     5161 gaggccctct tcctggccgg gcacctcgac gaggcgcggg tcgacctcga gcgctcgatg
     5221 gcgatgcacc aggacctgca cgccgacgcg ggcacggcgc tctgcctgca gcggctggcc
     5281 gaggtggagc tggccaacgg cgaccgcgag accgcagccc gccatgcccg ccaggccctg
     5341 agcctggccc ggtggtcccc cctcgcccgt cacctgatcc agcgcatcta cgggacgctg
     5401 atcgccatcg ccccggacgt cgacgccgcg gtcgcgttgg tggccgaggc cgagaacgcg
     5461 gccgacccgg cagcccgatg ccagtactgc gacatcatgc tggccgtgcc ggcgacggcc
     5521 gccctggccg aggccggtcg cctcgacgag gcccgttcga gcctggagcg ggcccagcga
     5581 gccgccgggc tgtggcacgg caccgcttgg cagggcgcgg tggccgaggc ggaggccgtc
     5641 ctggcccggg ccgagggtcg gggcgacgag gccgaggcgc tcctggcccg cgccgccggc
     5701 ctgttcgacg aggcgggtca gcccttggac gccgcccgct gtcgcgaagc cgcgatcggc
     5761 tgaggccggc gctggcctac gacgcgacgg cgacctcccg aggggcggag acgccgcggc
     5821 gggggacgag gatgtcgtcg atgaggcccc aggccttggc ctcctcggcg gtcatgaagt
     5881 agtcccggtc gaggtcgtgc tcgacctcct ccatggaccg gccgctgtga tggctcagga
     5941 tctcggtcat ccggtgcgtg gtggccagga cctcccgcag gtggatctcc atgtcccggg
     6001 gcgcaccccg ggcgccggcc gatccctggt ggatcatgag cttggcgctg gggagggcga
     6061 agcgcttgcc ctcggccccg ccgcacagga tcatcgccgc cgccgacatc cccatgccca
     6121 cgcaaatggt ggccacgtcg cagcggacgt gctgcatggt gtcgtagatg gccatgccgc
     6181 cgtaggccag gccaccgggg gagttgatgt agaggttgat gtccttgtcc gggtcctggg
     6241 cctcgaggtg caggatctgg gcgatgagca ggttggcgat ctggtcgtcc acctcctggc
     6301 ccaggaagac gatgcgctcg ctcagcaggc tggag
//
LOCUS       DASTFV010000625         5104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2387228, whole genome shotgun sequence.
ACCESSION   DASTFV010000625 DASTFV010000000
VERSION     DASTFV010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2387228"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..692)
                     /locus_tag="VFP02_11435"
     CDS             complement(222..692)
                     /locus_tag="VFP02_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9543691.1"
                     /translation="MGRWSREELEDAFAHYQRVALAAGTSGDWDPWADLFTEDATYIE
                     HLYGTLGGREAIRTWITATMAQPVNCDMRFFPIEWYVIDEERGWVVCQVWNRMIDPGD
                     GSLHQAYNFTLLKYAGDQRWSYEEDIYNPGHFKDMVMGWLDRRSSLAPGEADSP"
     gene            complement(692..1117)
                     /locus_tag="VFP02_11440"
     CDS             complement(692..1117)
                     /locus_tag="VFP02_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377081.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HET9543692.1"
                     /translation="MTRLRGPHGGPVRIVLAKLGLDGHDRGLKVVARMLRDAGAEVVY
                     LGLRQTTETVVAAAQQEDADLIGISMHNGGHGTLAPRMVEAARAAGLEAPVVVGGIIP
                     EADAEELRAAGVAVVLTPGASADDVVAAIAAALENTGGH"
     gene            complement(1114..2703)
                     /locus_tag="VFP02_11445"
     CDS             complement(1114..2703)
                     /locus_tag="VFP02_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET9543693.1"
                     /translation="MTDEDVPPQFETLSGIPVDPVYGDGPGPGEFPYTRGPYASMYRA
                     KPWTMRMFAGFGSAADTNQRFRQLLDSGGTGLSTAFDMPTLMGRDSDDSWAIGEVGKA
                     GVAIDTLDDMATLFDDIDLGAVSTSMTINGPAAIIMAMYVAVAEGQGTSRAALSGTIQ
                     NDILKEYQAQKEYLYPPRPSLRLVTDLIRFTTAEMPRFHPVSISGYHIREAGSTADQE
                     LAFTLANGFAYVEAATAAGLDVDDFAPRLSFFFNAHLDFFEEIGKFRAARRIWARWLR
                     DRYGATDERSLRLRFHTQTAGVSLTAQQPEVNIARVAIQALSAVLGGTQSLHTDAYDE
                     ALALPSETAARIALRTQQVIAHETGVTVTADPLGGSAYVEWMTDEMERRAEATFAHLE
                     GRGGGSMLEGVLAGIDNGWIVNEIADAAYKFERAVNAGDRVVVGVNRFTDGDERTADL
                     LRISADVEAAQVERLEATRRDRDQAAVDRALAAIGRDAAEPTVNLLPPMIDAVRVRAT
                     EGEIVHALEAEFGTYTERAAV"
     gene            2723..3427
                     /locus_tag="VFP02_11450"
     CDS             2723..3427
                     /locus_tag="VFP02_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543694.1"
                     /translation="MEKLVYLIWERPSVDGGAWRSRFLDDLAPRLLALGPHSLSLHLD
                     DDEAPIPGPAPAPGHELPLRAAVSVFVDAHDRRAPYEAVLADVGERRAGYLVSEALWS
                     EYGENGRRGPRDWADGARSPGITTFSLVHRNPNLDERTFREFWHGHQSPMSEAVQPRW
                     RYVRNTVVHPVTAGAPPIDGIVHESWPSIDVVEDTIAFHEGDAENITVMLDSVMQCFD
                     IARLRSFAFSEYLLTS"
     gene            complement(3431..>5104)
                     /locus_tag="VFP02_11455"
     CDS             complement(3431..>5104)
                     /locus_tag="VFP02_11455"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9543695.1"
                     /translation="ADHAIAWINDLRAADPDKPFFAWFCPGACHSPHQAPADWIEAYR
                     GRFEEGWDVWREAALERQKADGLLPGHTVLSPRPTWVPAWADLGEDERRVYERYMEAF
                     AGYLSHTDAQVGRIVDALAETGDLDNTIIVVVSDNGASSEGGAVGSLNDLRAWNGMDR
                     TVAEALDRIDDIGGPDIHNNYPWGWTVAGNTPFRRWKREVHEGGVADPMIVHWPAGIS
                     AAGEVRHQYVHAIDLAPTLLALVGVESPGVIDGIEQRPLEGASFAASLTDADAPDPRP
                     TQYYEMFGCRAIRHEQWKAVVYHPIQVDHPPLEEDTWELYDLEADPSETTDLADRHPD
                     VVADLVTRWWDEAERHHVLPLDNRPFSEFVLDRPPPVPPRQEYVYRPGTSMVPEATAV
                     NVRNRDHRIVADIDVPTVGDRVEGVLLAQGSILGGWSLHLLRGRLIYVHNNTGLHAAR
                     IEAPAFLAPGPHQLAFQYVRTSDHAGEGTLFVDGVKWGHGTVPHFTMNRFSITGNGLT
                     CGRSEGEPPCKDYRSPFPFSGTLHRVTVEVDGEPWLDPKTEAEDALRRQ"
BASE COUNT          715 a         1784 c         1813 g          792 t
ORIGIN      
        1 ccagcgaggc gccacggtcc gagtcgccga agcgagcccg cagccaagtg gcgaggaagt
       61 cgtaggacag catcatgttg gttctccgtt gattcgttcc cgccgccatg tggaggtggg
      121 gccttgttga caccaactcc atcggcgggt ttggctccca acgtgatagg tagttcgacc
      181 catttgcaaa aaaagtccga gtcgctccgg ggcctctaga cctacgggga gtcggcctca
      241 cccggggcga ggctcgagcg acggtccagc cagcccatga ccatgtcctt gaagtggccc
      301 gggttgtaga tgtcctcctc gtaggaccac cgctggtccc ccgcgtactt gagaagcgtg
      361 aagttgtacg cctggtgcag gctcccgtca ccgggatcga tcatgcggtt ccagacctgg
      421 cacacgaccc agccccgttc ctcgtcgatc acgtaccact cgatcgggaa gaatcgcatg
      481 tcgcagttca ccggctgggc catcgtcgcg gtgatccacg tgcggatggc ctcccgcccc
      541 ccgagcgtcc cgtagaggtg ctcgatgtag gtggcgtcct ccgtgaacag gtcggcccag
      601 ggatcccagt ccccactcgt gccggccgcg agggcgaccc gttggtagtg ggcgaaggcg
      661 tcctccagct cctcgcggct ccaccgaccc atcagtgccc tcccgtgttc tccaacgcgg
      721 ccgcgatcgc ggccacgaca tcgtccgccg acgcccccgg ggtcagcacc acggcgaccc
      781 cggcggccct gagctcctcg gcatcggcct cagggatgat gccgccgacc accaccgggg
      841 cctcgagccc ggcggcccga gccgcctcga ccatccgggg ggcgagcgtg ccgtggccgc
      901 cgttgtgcat gctgatgccg atcaggtcgg cgtcctcctg ctgggcggcg gcgaccaccg
      961 tctcggtggt ctggcgcagc cccaggtaca cgacctcggc gccggcgtcg cggagcatgc
     1021 gggccaccac cttcaggccg cggtcatggc cgtcgaggcc gagcttggcc agcacgatgc
     1081 ggacgggccc cccgtggggg ccccgcaggc gggtcacacc gcggcccgct cggtgtaggt
     1141 cccgaactcg gcttcgaggg cgtggacgat ctcgccctcg gtcgcccgca cccgcacggc
     1201 gtcgatcatc ggcggcagca ggttgaccgt cggctcggcg gcgtcccgac cgatggcggc
     1261 cagggcccgg tcgaccgcgg cctggtcccg gtcccggcgg gtggcctcga ggcgctcgac
     1321 ctgcgcggcc tcgacgtcgg cgctgatgcg cagcaggtcc gcggtgcgct cgtcaccgtc
     1381 ggtgaaccgg ttgacgccga ccacgacccg gtcgccggcg ttcacggccc gctcgaactt
     1441 gtaggccgcg tcggcgatct cgttcacgat ccagccgttg tcgatgccgg ccagcacccc
     1501 ttcgagcatc gaaccgccgc cacggccttc gaggtgggcg aaggtggcct cggcccggcg
     1561 ctccatctcg tcggtcatcc attcgacgta ggccgagccc ccgagggggt cggcggtgac
     1621 cgtcaccccc gtctcatggg cgatgacctg ctgggtgcgc agggcgatgc gggccgccgt
     1681 ctccgacggg agggcgaggg cctcgtcgta ggcgtcggtg tgcaggctct gcgtgccccc
     1741 gaggacggcg gacagggcct ggatggcgac ccgggcgatg ttcacttcgg gctgctgggc
     1801 cgtgagggac acgccggcgg tctgggtgtg gaaccggagg cgcagcgagc gctcgtcggt
     1861 ggccccgtac cggtcgcgca gccagcgggc ccagatccgc cgagccgccc ggaacttgcc
     1921 gatctcctcg aagaagtcga ggtgggcgtt gaagaagaag gagagccggg gggcgaagtc
     1981 gtccacgtcc aggcccgccg ccgttgccgc ctccacatag gcgaacccgt tggccagcgt
     2041 gaacgccagc tcctggtccg ccgtcgagcc cgcctctcgg atgtggtagc cggagatgga
     2101 cacgggatgg aaccggggca tctcggccgt ggtgaagcgg atcaggtcgg tgaccaggcg
     2161 cagcgacggc cggggcgggt acaggtactc cttctgggcc tggtactcct tgaggatgtc
     2221 gttctggatc gtgcccgaca gggcggcccg ggacgtgccc tggccttcgg ccacggccac
     2281 gtacatcgcc atgatgatgg cagcggggcc gttgatggtc atcgacgtgg acaccgcgcc
     2341 caggtcgatg tcgtcgaaca gagtggccat gtcgtcgagg gtgtcgatgg cgacgccggc
     2401 cttgcccacc tcgccgatgg cccaggagtc gtcggagtcc cgccccatca gggtgggcat
     2461 gtcgaaagcc gtcgacaggc cggtgccccc cgagtccagg agctggcgga agcgctggtt
     2521 ggtgtccgcc gccgacccga agccggcgaa catccgcatc gtccacggct tcgcccggta
     2581 catggaggcg tacggcccgc gggtgtaggg gaactcgccg ggaccggggc cgtcgccgta
     2641 gacgggatcg accgggatgc ccgacagggt ctcgaactgc ggtggtacgt cctcgtcggt
     2701 caccgtgaca gtattccgcg ccgtggagaa gctcgtgtac ctcatctggg aacgcccctc
     2761 tgtcgacggc ggagcctggc ggtcccgctt cctcgacgac ctcgcccccc ggctcctggc
     2821 cctcggcccc cacagccttt ccctgcacct cgacgacgac gaggccccga tccccggacc
     2881 ggcccccgcc cccggccacg agctgcccct ccgggctgcg gtgtccgtgt tcgtcgacgc
     2941 ccacgaccgc cgggcgccct acgaagccgt cctggccgac gtcggtgaac gacgggcggg
     3001 ctacctggtc agcgaggcgc tgtggtccga gtacggcgag aacgggcgtc ggggacctcg
     3061 ggactgggcc gacggcgcgc gctcccccgg catcaccacc ttctccctgg tccaccgcaa
     3121 cccgaacctc gacgagcgca ccttccggga gttctggcac ggccaccagt cgccgatgtc
     3181 cgaggccgtc cagccccgct ggcgctacgt ccgcaacacc gtggtccacc cggtgaccgc
     3241 cggcgccccc ccgatcgacg ggatcgtgca cgagtcctgg ccctccatcg acgtggtcga
     3301 ggacacgatc gcgttccacg agggcgatgc ggagaacatc acggtgatgc tggacagcgt
     3361 catgcagtgc ttcgacatcg cccgtctccg gtcgttcgcc ttcagcgagt atctgttgac
     3421 gagctgacca ctactgacgg cggagcgcgt cctcggcttc ggtcttgggg tcgagccagg
     3481 gctcgccgtc gacctcgacg gtgacccggt ggagcgtccc gctgaacggg aagggcgagc
     3541 ggtagtcctt gcagggcggc tcgccctcgg accggccgca ggtgagcccg ttgcccgtga
     3601 tcgagaagcg gttcatcgtg aagtgcggga ccgtgccgtg accccacttc accccgtcga
     3661 cgaagagcgt cccctcaccg gcgtggtccg acgtgcgcac gtactggaag gccagctggt
     3721 ggggcccggg ggcgaggaac gccggggctt cgatgcgtgc cgcgtgcagg cccgtgttgt
     3781 tgtgcacgta gatcaaccgg ccccggagga ggtggaggga ccagccgccg aggatcgagc
     3841 cctgggccag gagcacgcct tcgacccggt ccccgaccgt cggcacgtcg atgtcggcga
     3901 cgatgcgatg gtcccggttg cgcacgttca ccgcggtcgc ctcggggacc atcgaggtcc
     3961 ccggccggta gacgtactcc tgtcgtgggg gcacgggcgg cgggcggtcc aggacgaact
     4021 cggagaacgg tcggttgtcc agggggagca cgtgatgccg ctcggcctcg tcccaccacc
     4081 gggtcaccag gtcggccacg acgtcgggat gccggtcggc gagatcggtc gtctcggagg
     4141 gatcggcctc caggtcgtag agctcccagg tgtcctcctc cagcggcggg tggtccacct
     4201 ggatggggtg gtagacgact gccttccact gctcgtggcg gatggctcgg cagccgaaca
     4261 tctcgtagta ctgggtcggg cgcggatcgg gggcatcggc gtcggtcagg gaggcggcga
     4321 agctggcccc ctccagggga cgctgctcga tcccgtcgat cacgcccggc gactcgacgc
     4381 ccacgagggc gagcagggtc ggcgccaggt cgatggcgtg cacgtactgg tggcggacct
     4441 caccggcggc actgatgccg gcgggccagt gcacgatcat cgggtcggcc accccgcctt
     4501 cgtggacctc ccgcttccaa cgacggaagg gcgtgttgcc ggcgaccgtc caaccccagg
     4561 ggtagttgtt gtggatgtcg ggcccgccga tgtcgtcgat gcggtccagc gcctccgcga
     4621 ccgtgcggtc catgccgttc caggcccgca ggtcgttcag ggagccgacc gctccgccct
     4681 ccgagctggc tccgttgtcg gacacgacca cgatgatcgt gttgtcgagg tcgccggtct
     4741 cggccagcgc gtcgacgatc cggcccacct gggcgtcggt gtgggagagg tagccggcga
     4801 aggcctccat gtaacgctcg tagacgcggc gctcgtcctc gccgaggtcc gcccaggccg
     4861 ggacccaggt cgggcggggg gacaggacgg tgtggccggg cagcagccca tcggccttct
     4921 gacgctcgag ggcggcctcc cgccagacgt cccacccctc ctcgaagcga ccccgatagg
     4981 cctcgatcca gtccgccggg gcctggtggg gtgagtggca cgcccccggg cagaaccagg
     5041 cgaagaacgg cttgtcggga tccgccgccc gcaggtcgtt gatccaggcg atggcgtggt
     5101 cggc
//
LOCUS       DASTFV010000626         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2390056, whole genome shotgun sequence.
ACCESSION   DASTFV010000626 DASTFV010000000
VERSION     DASTFV010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2390056"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VFP02_11460"
     CDS             complement(<1..801)
                     /locus_tag="VFP02_11460"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET9543696.1"
                     /translation="MRVLAAPDKFRGTATAPEVAAAMARAAVDAGGTADEAPLADGGE
                     GTLEALGGANRTTTVTGPLGDPVEAVWRLDRRAAVIEMARASGLDVVGGPEGNDAVAA
                     STYGTGELIAAAVERGAREIVVAVGGSATTDGGLGALRALEPSQRLRGIEIRVACDVR
                     LPFVDAAEVYGPQKGASPAQVELLRRRLERLAQIYEEEYGVDVTRLAGAGAAGGLAGG
                     LAAIGAHLEPGFDLVADAIDLDERMEGCDLVVTGEGFLDEQSFDGKVVG"
     gene            820..>2040
                     /locus_tag="VFP02_11465"
     CDS             820..>2040
                     /locus_tag="VFP02_11465"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HET9543697.1"
                     /translation="MVDRPSAGSAARPLVMVSNRGPVSFRHDDAGEIVARRGAGGLVS
                     GIGPLMAGTGAVWVAAAMSDADREVAATGVTDVEGFRFRALAVDPDAYRAFYDVIANA
                     ALWFVHHGLYDRARRPVVDRQWWAAWAAYGTVNQAFAEGVAEVAPEGAVVLVQDLHLC
                     LLAEPLAVSRPDLDLVHFSHTPFAGPDDLRVLPDDARTALLRGMAGHRACGFHTARWR
                     DAFVASCRADGVEPPATFVSPLGPDVDDIGGVAAGPACAAALADIEETLGGRRFIARV
                     DRIELSKNLIRGFLAYEDLLDRRPEWREGVVFGAYVYPSREGLPEYLAYRTEVEGIVA
                     RINDRFATADWTPVLYDATDDFPRSVAALRRADVVLVNPIRDGLNLVAKEAAVVNQRD
                     GVLVLSPEAGTWDEL"
BASE COUNT          236 a          794 c          734 g          276 t
ORIGIN      
        1 gccgaccacc ttgccgtcga aggactgctc gtccaggaaa ccctcgccgg tgacgacgag
       61 gtcgcagccc tccatccgct cgtcgaggtc gatggcgtcg gcgacgagat cgaagccggg
      121 ctcgaggtgg gcgccgatcg cggccaagcc gccggccagg ccgcccgccg cccccgcccc
      181 cgccaacctg gtgacgtcga cgccgtactc ctcctcgtag atctgggcca ggcgctcgag
      241 gcggcggcgc agcagctcca cctgagccgg gctggcccct ttctgggggc cgtagacctc
      301 ggccgcgtcg acgaacggca ggcggacgtc gcaggccacc cggatctcga tcccccggag
      361 ccgttgggaa ggctccagcg cccgcagcgc ccccagtccg ccgtccgtgg tcgccgaccc
      421 gcccacggcc acgacgatct cgcgggcgcc ccgctcgacg gcagccgcga tcagctcgcc
      481 ggtgccgtag gtcgaggcgg cgacggcgtc gttgccctcg gggccgccga ccacgtcgag
      541 tccggaggcc cgggccatct cgatgaccgc ggcccgacgg tcgaggcgcc agacggcctc
      601 gaccgggtcc ccgaggggac cggtcaccgt cgtggtgcgg ttggccccgc ccagcgcctc
      661 cagggtcccc tcgccaccgt cggccagtgg ggcctcgtcg gcggtcccac cggcgtccac
      721 cgccgcccgg gccatggcgg cggccacctc gggtgcggtc gcggtgccac ggaacttgtc
      781 gggcgcggcc aggactcgca ccctcctatg gtgccccgca tggtcgaccg accctcggcg
      841 ggttccgcgg cgcgcccgct ggtgatggtc tccaatcggg gtccggtgtc gttccgtcac
      901 gacgacgcgg gcgagatcgt ggcccgccgg ggcgccgggg ggctggtgtc gggcatcggc
      961 ccactgatgg cgggcaccgg ggccgtctgg gtcgccgccg ccatgtccga cgccgaccgc
     1021 gaggtggcgg cgaccggggt caccgacgtg gagggcttcc ggttccgggc cctcgccgtc
     1081 gacccggacg cctaccgggc cttctacgac gtcatcgcca acgccgccct ctggttcgtc
     1141 caccacggcc tctacgaccg ggcccggcgg ccggtcgtcg accggcagtg gtgggcggcc
     1201 tgggccgcct acggcaccgt gaaccaggcc ttcgccgagg gcgtggccga ggtggcgccg
     1261 gagggggccg tggtcctcgt ccaggacctc cacctctgcc tgctggcgga gcccctcgcc
     1321 gtcagccgcc ccgatctcga cctggtgcac ttcagccaca cgcccttcgc cggcccggac
     1381 gacctacggg tcctcccgga cgacgcccgc acggcgctgc tgcggggcat ggccgggcac
     1441 cgggcctgtg gcttccacac cgcccgctgg cgggacgcct tcgtggcctc gtgccgggcc
     1501 gacggtgtcg agcccccggc caccttcgtc tccccccttg gccccgatgt cgacgacatc
     1561 ggcggcgtgg ccgcggggcc ggcgtgcgcg gcggccctgg ccgacatcga ggagacgctg
     1621 gggggccgcc gcttcatcgc ccgggtcgac cgcatcgagc tgtcgaagaa cctgattcgg
     1681 gggttcctgg cctacgagga cctgctcgac cgacgacccg agtggcggga gggggtcgtc
     1741 ttcggggcct acgtgtaccc gtcccgcgag ggcctgcccg agtacctcgc ctaccggacc
     1801 gaggtcgagg ggatcgtcgc ccggatcaac gaccgcttcg ccacggccga ctggaccccg
     1861 gtcctctacg acgccaccga cgacttcccg cggtccgttg ccgccctgcg gcgggccgac
     1921 gtggtgctgg tcaacccgat ccgcgacggc ctgaacctgg tggccaagga ggccgcggtc
     1981 gtgaaccaac gcgacggcgt gctcgtcctg tcccccgagg ccgggacgtg ggacgagcta
//
LOCUS       DASTFV010000627         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2391868, whole genome shotgun sequence.
ACCESSION   DASTFV010000627 DASTFV010000000
VERSION     DASTFV010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2391868"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /gene="groL"
                     /locus_tag="VFP02_11470"
     CDS             <1..1187
                     /gene="groL"
                     /locus_tag="VFP02_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799237.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET9543698.1"
                     /translation="ISAADEEIGKMIADAIDKVGKDGVITVEESQTFGMEMDLVEGMR
                     FDKGYISPYFVTDPDRMEVELEDPYILLVSSKISAVKDMLAVLEKVMQAGKPLVIVAE
                     DVEGEALATLVVNKIRGTFKSVAIKAPGFGDRRKAMLQDLAILTGGQVISEEVGLKLD
                     NTTLDLLGSARKVVVTKDEATIVEGAGDEKDIQARIQQIKNEIDNTDSDYDREKLQER
                     LAKLSGGVAVLKVGAATEVELKEKKHRIEDAVSTTKAAIEEGVVTGGGVTLIRASEAV
                     DKVVKKLEKSEHDEATGARLVQRAMSEPLRQIAVNAGLEGGVVVEKVRDLPNPTEGLD
                     AATGEYGDLMKLGIIDAAKVTRSALQNAASIAALFLTTEAVIADKPEEEPAMPGGGDM
                     DF"
     gene            1324..1800
                     /locus_tag="VFP02_11475"
     CDS             1324..1800
                     /locus_tag="VFP02_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HET9543699.1"
                     /translation="MTTPPLHPALGPLAFLLGEWAGTGAGSYPTDIRDFRYHEEVRFW
                     HRGKPVLLYTQRTVDLDDGAPLHAEMGFWRPQPDDRVEVVISHPTGHAEVSAGSVVDG
                     RVELATTAVTATGTAKEVTAVERTFTVAGDELAYELRMAAVGRPMTHHLAATLRRA"
     gene            1853..2650
                     /locus_tag="VFP02_11480"
     CDS             1853..2650
                     /locus_tag="VFP02_11480"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HET9543700.1"
                     /translation="MRGRTVTALTHRFRDGAVARVPDALIVEEPLAIDLDGTLVATTM
                     RTPGHDFELAVGFCFTDGLLAGAPVLRTRYCGTGSAVETEFNAVSVETGGKAPVPTPR
                     LTATTSSCGLCGSATLDELVDRLDPLPAVEPFPPEVLLEVTDKAAVDQDLFAATGAVH
                     AAAAFGRDGSPFLLREDIGRHNAVDKVVGRLLLDGALPAMDLGLFVSGRASFEIVAKA
                     WAAGFAAVVAVSAPSALAVDTARAAGLQLAGFARDGQINLYAPEDMA"
     gene            2662..>3476
                     /locus_tag="VFP02_11485"
     CDS             2662..>3476
                     /locus_tag="VFP02_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:2932..2934,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9543701.1"
                     /translation="MPGRNRRDRRAPGARLERPLVREDGVLRPATWEEALDRATEGFR
                     RVLDTQGPTGIGLFSCSKATNEVNFAAQKFARTALGSNYIDSCNRTUHAPSVVGLATV
                     FGAGGGTCSYEEVEEADVVLLWGSNAREAHPIYFHHLLKGIENGARLYCVDPRRTSSA
                     KFADVWLGLEVGSDIALANALGREIITAGLTDDAFIAHSTQGYDAYAAAVEPYTLEAA
                     AALTGLPADAIRDMAHAYAQAEKAQILWTLGITEHHNAVDNVVALCNLALLTG"
BASE COUNT          549 a         1249 c         1188 g          490 t
ORIGIN      
        1 ccatctccgc cgccgacgag gagatcggca agatgatcgc cgacgccatc gacaaggtgg
       61 gcaaggacgg cgtcatcacc gtcgaggagt cccagacctt cggcatggag atggacctgg
      121 tcgagggcat gcgcttcgac aagggctaca tctcgccgta cttcgtcacc gaccccgatc
      181 gcatggaggt cgagttggag gatccctaca tcctcctggt ctcctccaag atctcggccg
      241 tcaaggacat gctggccgtg ctcgagaagg tcatgcaggc gggcaagccg ctggtcatcg
      301 tggccgagga cgtcgagggc gaggccctgg ccaccctcgt ggtcaacaag atccgcggca
      361 ccttcaagtc ggtggccatc aaggcccccg gcttcggcga ccgccgcaag gcgatgctgc
      421 aggacctcgc catcctcacc ggcggccagg tcatctccga ggaggtcggg ctcaagctcg
      481 acaacaccac gctcgacctg ctgggctcgg cccgcaaggt cgtggtgacc aaggacgagg
      541 ccaccatcgt cgagggcgca ggtgacgaga aggacatcca ggcccgcatc cagcagatca
      601 agaacgagat cgacaacacc gacagcgact acgaccgcga gaagctccag gagcgcctcg
      661 ccaagctgtc cggtggagtc gccgtcctga aggtcggcgc cgccaccgag gtcgagctca
      721 aggagaagaa gcaccgcatc gaggacgccg tcagcacgac gaaggcggcc atcgaggagg
      781 gtgtcgtcac cggcggtggc gtgaccctga tccgggcctc cgaagcggtc gacaaggtcg
      841 tcaagaagct cgagaagagc gagcacgacg aggccacggg cgcccggctg gtccagcggg
      901 ccatgtccga gcccctccgc cagatcgcgg tcaacgccgg cctcgagggc ggcgtcgtgg
      961 tcgagaaggt gcgggacctc ccgaacccga ccgagggcct ggacgcggcc accggcgagt
     1021 acggcgacct catgaagctc ggcatcatcg atgccgcgaa ggtgacccgg tcggccctgc
     1081 agaacgcggc gtccatcgcg gcgctcttcc tgaccaccga agcggtcatc gccgacaagc
     1141 ccgaagagga gccggccatg cccggcggcg gggacatgga cttctgatct ctgcgcctct
     1201 cggcgcggcc cctgacgggg atgaggtcgt tgctgacccc agctcgtcgg tttgtgcaaa
     1261 tcagcgacct gtcggcggtg acgtcggcag gttgctgtcg ttttcgggcc tagcgtcggg
     1321 gcagtgacga caccgccgct gcatcctgcg ctcgggccgc tggccttcct gctcggggaa
     1381 tgggccggca cgggagcggg ctcgtacccg actgacatcc gcgacttccg ttaccacgag
     1441 gaggtccggt tctggcaccg gggcaagccg gtgctgctct acacccagcg cacggtcgat
     1501 ctcgacgacg gggcgccgct ccatgccgag atgggcttct ggcgcccgca gcccgatgac
     1561 cgggtggagg tggtgatcag ccacccgacg ggccacgccg aggtcagcgc tggctccgtc
     1621 gtcgacggac gggtcgagct ggccacgacc gcggtcacgg cgacgggcac ggccaaggag
     1681 gtgacggcgg tcgaacggac cttcaccgtg gccggcgacg agctggccta cgagctgcgc
     1741 atggcggccg tgggtcggcc gatgacccac cacctcgccg ccaccctgcg gcgggcgtga
     1801 cccgcacgcg gcgtgccccc gccgtcgatg ggcggtcggt agcctcgatc cggtgagggg
     1861 acggacggtc acggcgctga cgcaccgctt ccgcgacggg gccgtggccc gggtccctga
     1921 cgccctcatc gtcgaggagc cgctggccat cgacctggac gggacgctgg tggccacgac
     1981 catgcgcacg ccgggccacg acttcgagct ggcggtcggc ttctgcttca ccgacggcct
     2041 cctcgccgga gctccggtcc tgcgcacccg ctactgcggg acgggctcgg cggtggagac
     2101 cgagttcaac gccgtgtccg tcgagacggg ggggaaggcg ccggtcccga ccccccgcct
     2161 gaccgccacc acgtcgagct gtggcctctg cggatcggcc accctcgacg aactggtcga
     2221 ccgcctcgac ccgctgcccg cggtcgagcc cttcccgccg gaggtcctcc tggaggtcac
     2281 cgacaaggcg gccgtcgacc aggatctctt cgccgccacc ggggccgtcc acgccgccgc
     2341 cgccttcggt cgagacggca gcccgttcct gctccgggag gacatcggtc gtcacaacgc
     2401 cgtggacaag gtggtcggcc ggctgctcct ggacggcgcc ctgccggcca tggacctcgg
     2461 cctgttcgtc agcggacggg cctccttcga gatcgtggcg aaggcctggg cggctgggtt
     2521 cgccgccgtg gtcgccgtca gtgccccctc cgccctcgcc gtcgacaccg cgcgggccgc
     2581 cggtctgcag ctcgcggggt tcgcccggga cggccagatc aacctgtatg cccccgagga
     2641 catggcctag ccttggcgcc gatgcccgga cggaaccgac gcgatcgacg ggcgcccggg
     2701 gcccgcctcg aacggcccct ggtccgcgag gacggtgtgc tccgccccgc cacctgggag
     2761 gaggctctgg accgggccac cgaaggcttt cgccgggtgt tggacaccca gggccccacc
     2821 ggcatcggcc tgttcagctg ctcgaaggcc accaacgagg tcaacttcgc ggcgcagaag
     2881 ttcgcccgta ccgccctggg ctccaactac atcgattcct gcaacagaac ctgacacgcg
     2941 ccatccgtcg tcggtctggc gacagtcttc ggtgccggtg gtgggacctg ctcgtacgag
     3001 gaggtggagg aggccgacgt cgtcctcctc tggggcagca acgcccgtga ggcgcacccg
     3061 atctacttcc accacctgct gaaggggatc gagaacgggg cccggctcta ctgcgtggac
     3121 ccccgccgta cgtcgtcggc caagttcgcc gacgtgtggc tgggcctgga ggtgggctcg
     3181 gacatcgccc tggccaacgc cctcggccgc gagatcatca ccgccggcct gaccgacgac
     3241 gccttcatcg cccactcgac ccagggttac gacgcctacg ccgccgccgt cgagccgtac
     3301 acgctcgagg cggccgcggc cctgaccggg ctgcccgccg acgccatccg ggacatggcc
     3361 cacgcctacg cccaggccga gaaggcccag atcctctgga cgctgggcat caccgagcac
     3421 cacaacgcgg tcgacaacgt ggtcgcgctc tgcaacctgg ccctgctcac cggcca
//
LOCUS       DASTFV010000628         7084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2392604, whole genome shotgun sequence.
ACCESSION   DASTFV010000628 DASTFV010000000
VERSION     DASTFV010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2392604"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1184)
                     /locus_tag="VFP02_11490"
     CDS             complement(201..1184)
                     /locus_tag="VFP02_11490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET9543702.1"
                     /translation="MMRLALQVPNFTYPDVEDGDLFETVAGVAAAAEASGFDTLLVMD
                     HFYQLPMLGSPDQPMFEAYTLLSALAARTSSIQLGTLVTGVTYRNPAFLAKVVTNLDV
                     ISRGRAMLGIGAAWFDMEHDAFGYDFPPVSDRFEMLEEAIQITQAMFADERPTFEGKH
                     FQVRDIINNPKPIRAKIPLMVGGAGEKKTLRIAAQYADHSNLTCGLDEVPHKLDVLAG
                     HLADVGRERSEIGVSALNFLIAGDTDDEAFASRDELVRGLGMEWDTLDDATREMLGHR
                     MLVGGPDSIGEQVQERILGQGLDAVVVNIPTGGHLPEVVARSGEILTKALS"
     gene            complement(1230..1790)
                     /locus_tag="VFP02_11495"
     CDS             complement(1230..1790)
                     /locus_tag="VFP02_11495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HET9543703.1"
                     /translation="MADVRSWLKDAAAGFGSCLEGVADDQWSSPTPDAEWDVRALVAH
                     LVDEQLWAPPLLAGRTIEDIGDEIPSDPLGDDPVAAQRSATAAMVDALEDLDLDATVH
                     LSFGDVPAQEYLMQLFADHLIHTWDLARATGQPERLDPELVAVCATWFVGREDLYRQS
                     GAIGPAVTVEGGDAQSELLGRFGRRP"
     gene            complement(1810..2469)
                     /gene="nucS"
                     /locus_tag="VFP02_11500"
     CDS             complement(1810..2469)
                     /gene="nucS"
                     /locus_tag="VFP02_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HET9543704.1"
                     /translation="MRLVIARCQVDYEGRLTAHLPMAPRLIMVKADGCVAIHADGGAY
                     KPLNWMNAPNTLVEGDGVWTVTSPKGEQLIITMEEILSDSAHELGIDPGLTKDGVEAH
                     LQELLALDCNRIENGFKLVRREWPTDIGPVDLLCRDADGQAVAVEVKRRGEIDGVEQL
                     TRYLERMDRDPSLRPVRGVFVAQVIKPQAKVLAESRGIACVEVDYDELRGIERNELKL
                     F"
     gene            2509..2682
                     /locus_tag="VFP02_11505"
     CDS             2509..2682
                     /locus_tag="VFP02_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543705.1"
                     /translation="MHISERWEYLTIVDIPTTQLDGVKESYAELGYEAVAEDTDRLVV
                     RKEFPVTGQITVD"
     gene            2709..3347
                     /locus_tag="VFP02_11510"
     CDS             2709..3347
                     /locus_tag="VFP02_11510"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HET9543706.1"
                     /translation="MDPPEVAGDLDAVAFSDLTGGDEVEDALVTGELVGGSLDGLTLA
                     GCRLDGVRLTGSSFDRLVLEDVILVGCELSGVTLGATKLRRVRFEDCRMSGLSTADLH
                     AEHVSFVENRLDDAWFRMADIDHAEFLDCDLRRADFYAATVRRTRFLGCDLTGAEFSR
                     AVVTALRLERSTVYELGGIGGLRDIVLSPDQVVSFALAFLASFDITVDDPDD"
     gene            complement(3404..5323)
                     /gene="cysN"
                     /locus_tag="VFP02_11515"
     CDS             complement(3404..5323)
                     /gene="cysN"
                     /locus_tag="VFP02_11515"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HET9543707.1"
                     /translation="MSHQSELIATDIEAYLAEHEQKDLLRFLTCGSVDDGKSTLIGRL
                     LHDSHLLYEDQLAAVEAESTTIGSAGGELDLALLMDGLKAEREQGITIDVAYRYFSTS
                     RRKFIIADTPGHEQYTRNMVTGASTCDLAIILVDARHGVQAQTKRHSYLTSLLGIENT
                     VVAINKMDLVDHSKERFEEIRDHYLEFAERLDLKKAPWFIPISALKGDWVVDPGTNMS
                     WFDGPTLMEHLETVPVTVPEDGDLRFPVQLVRRPDLTFRGFAGTVASGVVHPGDEVMV
                     LPSGVVTTVDRLVTFNGDLDEAGPGTALTMTLTDEVDVSRGDVLVHPERRPQRSHEVD
                     AMVVWMSETDMAPGKEYLVQQANRIVPGRITTMAHRIDINTLEPEAAAGLAVNEIGRC
                     TVTCDQQLLFDAYERNRTTGAFILVDRLSNATMAAGMILPQDSAWDVQPDEHLQRHLS
                     QITVAERESRYGQRPCTVLLTGLTGAGKSTTAVALERRLFDAGRATLRIDGEDLRLGM
                     SRDLGFSAQERSENLRRMAEVARLANESGIIVIAAFAAPKADVRDRARELVGPERFLI
                     VHLDAPVEVARQRDPEGLYEAADRGEIPQFPGVSATYDVPENPDLTLDTATVDQADNV
                     ERIIDLLTERGFLTP"
     gene            complement(5324..6250)
                     /gene="cysD"
                     /locus_tag="VFP02_11520"
     CDS             complement(5324..6250)
                     /gene="cysD"
                     /locus_tag="VFP02_11520"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HET9543708.1"
                     /translation="MTADALPDAPRRTHLQQLEDESIHIIREVVAQFERPVMLYSIGK
                     DSGVMLRLAEKAFHPGKLPFPLLHVDTTWKFKDMIAHRNRMAEELGVELIVHSNQDGV
                     AAGINPFDHGSKNYTDIMKTQALKQALDAGGYDAAFGGARRDEEKSRAKERVFSFRDK
                     HHRWDPKSQRPELWNFYNGRVHPGESIRVFPLSNWTELDIWQYIWLEEIPLVPLYYSA
                     LRPTVERDGTLIMVDDDRFRFEPGEEPVDRMIRFRTLGCYPLSGAVESTATTLPQIIQ
                     EMLLATRSEREGRVIDHDQAGSMEEKKKEGYF"
     gene            complement(6259..>7084)
                     /locus_tag="VFP02_11525"
     CDS             complement(6259..>7084)
                     /locus_tag="VFP02_11525"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HET9543709.1"
                     /translation="VLVMERLSGFALDEVDAMKAAGVDLMALIRTGIIGFLEGAMFHG
                     VFHGDLHGGNLFVQPDGRTALLDFGITGRLDEPRRLAFLKLLAGGSTNDVRVQLPALA
                     ELGALPLDTDFEALIAELGLDAPAVDPTQMDPDEMVGELQRVLKALLGYGARMPKELM
                     LFVKNMAFSTSMGQTLAPDLDILELIQQVIVHFATTHGERIAADLQQEYDPDAYLAEV
                     SLDGYRASLGMPDAEAITLAELQARRRLIRDRLSKRSRKGGAHYTPTGPEKDPVGR"
BASE COUNT          998 a         2322 c         2583 g         1181 t
ORIGIN      
        1 gagccggtcc atgagatcag tcaggcgcgc ccgccgctcg gcgtcccgct cgacgacgaa
       61 cacccagggc tgggtgttcg tcgagctcgg cgcgaagcgg gccgcatcca gcacgcgttc
      121 tacgaggtcg tcaccgacct cgcggtcggg gtcgaaggcg cggcaggccc gctgccggcc
      181 gacgacgtcg aagaagtccg tcaggagagg gccttggtca ggatctcgcc gctccgggcg
      241 acgacctcgg gcaggtggcc accggtcggg atgttgacca ctacggcatc gaggccctgg
      301 cccaggatcc gctcctggac ctgctcgccg atgctgtcgg ggccgccgac cagcatgcgg
      361 tggccgagca tctcccgggt ggcgtcgtcc agggtgtccc actccatccc caggccccgg
      421 accagctcgt cccgggaggc gaaggcctcg tcgtcggtgt cgcccgcgat caggaagttc
      481 agggcgctga ccccgatctc gctgcgctcc cggccgacgt cggccaggtg accggccagc
      541 acgtccagct tgtgcggcac ctcgtcgagt ccgcaggtca ggttcgagtg gtcggcgtac
      601 tgggcggcga tgcgcagggt cttcttctcg ccggccccgc cgaccatcag ggggatcttg
      661 gcgcggatcg gcttcgggtt gttgatgatg tcccggacct ggaagtgctt gccctcgaag
      721 gtcggccgct cgtcggcgaa catggcctgg gtgatctgga tggcctcctc cagcatctcg
      781 aagcggtccg acaccggcgg gaagtcgtag ccgaaggcgt cgtgctccat gtcgaaccag
      841 gccgctccga tgccgagcat ggcccgaccc cgggagatga cgtcgaggtt cgtgacgacc
      901 ttggccagga aggccgggtt ccggtaggtc acgcccgtca ccagggtgcc cagctggatg
      961 gagctcgtcc gggcggccag cgcgctgagc agggtgtacg cctcgaacat cggctggtcg
     1021 ggcgacccca gcatgggcag ctggtagaag tggtccatca ccagcaaggt gtcgaaaccc
     1081 gacgcctcgg cggcggccgc caccccggcc acggtctcga acaggtcgcc gtcctcgacg
     1141 tccgggtacg tgaagttcgg gacctggagg gcgagtcgca tcatggaggg atgtctatcc
     1201 cttcacgcgg gagaacttct gtcgtcgggc taggggcggc ggccgaagcg gccgaggagc
     1261 tcggactggg cgtcgccgcc ctcgacggtc acggccggtc cgatggcccc gctctggcgg
     1321 tagaggtcct cccgtccgac gaaccaggtg gcacacacgg ccacgagctc gggatccagg
     1381 cgttcgggct ggccggtggc ccgggccagg tcccacgtgt ggatcaggtg gtcggcgaac
     1441 agctgcatca ggtactcctg ggccggcacg tcaccgaagg acaggtgcac ggtggcgtcg
     1501 agatcgaggt cctccaaggc gtccaccatc gcggcggtgg cgctgcgctg ggcggcgacg
     1561 gggtcgtcgc ccagcgggtc ggacgggatc tcgtcaccga tgtcctcgat ggtgcgtccg
     1621 gccagcagcg ggggcgccca gagctgctcg tccacgaggt gggcgaccaa cgcccgcacg
     1681 tcccattcgg cgtcgggggt gggggaggac cactggtcgt cggccacgcc ctccaggcag
     1741 gagccgaacc ctgctgccgc atccttcaac cagctcctca cgtccgccat ggcggtcccc
     1801 cttctccggt cagaacagct tcagctcgtt gcgctcgatg cctcgcagct cgtcgtagtc
     1861 gacctcgacg caggctatgc cccgggactc ggccaggacc tttgcctgtg gcttgatcac
     1921 ctgagcgacg aagacgccgc gtaccgggcg gagggacggg tcccggtcca tccgctcgag
     1981 gtagcgggtc agctgctcga ccccgtcgat ctcgccgcgg cgcttgacct ccacggccac
     2041 ggcttgcccg tcggcgtccc ggcagaggag gtcgaccgga ccgatgtcgg tcggccactc
     2101 ccggcggacg agcttgaacc cgttctcgat gcggttgcaa tcgagggcca gcagctcctg
     2161 gagatgggcc tcgacgccgt ccttggtcag gcccgggtcg atccccagct cgtgggccga
     2221 atccgacagg atctcctcca tggtgatgat cagctgctcg cccttggggc tggtcacggt
     2281 ccacaccccg tcgccttcca cgagcgtgtt gggcgcgttc atccagttca gcggcttgta
     2341 ggcgccgccg tcggcgtgga tggcgacgca cccgtcggcc ttcaccatga tcaggcgggg
     2401 ggccatcggc aagtgcgccg tgagccggcc ctcgtagtcc acctggcacc gggcgatcac
     2461 gagcctcacg gcgggcgagc gtaggcctgc ggtgcctacg atcgccccat gcacatctcg
     2521 gagcgctggg agtacctcac catcgtggac atccccacca cccagctcga cggggtgaag
     2581 gagagctacg ccgagctggg ctacgaggcg gtggccgagg acaccgaccg cctcgtcgtc
     2641 cgcaaggagt tccccgtcac cggccagatc accgtcgatt gaccccgccc gcgggcggtc
     2701 ccgctcccgt ggacccgccc gaggtggccg gcgacctcga cgcggtcgcg ttctccgacc
     2761 tgaccggagg ggacgaggtc gaggacgcgc tcgtcaccgg cgagctcgtc gggggctcgc
     2821 tcgacgggct cacgctcgcc ggctgccggc tcgacggggt gcggttgacc gggtcctcct
     2881 tcgaccggct ggtcctcgag gacgtcatct tggtgggctg cgagctgtcc ggggtgacct
     2941 tgggcgccac gaagctccgg cgggtccggt tcgaggactg ccggatgtcg ggcctctcga
     3001 ccgccgatct ccacgcggag cacgtcagct tcgtcgagaa tcgcctcgat gacgcctggt
     3061 tccggatggc ggacatcgac cacgccgagt tcctggactg cgacctccgc cgggccgact
     3121 tctacgccgc caccgtccgc cggacccggt tcctcgggtg tgacctgacg ggcgcggagt
     3181 tctcgagggc cgtggtcacg gccctgcggc tggaacgctc gacggtctac gagctgggtg
     3241 gcatcggcgg cctgcgcgac atcgtcctca gccccgatca ggtcgtgtcg ttcgccctgg
     3301 cgttcctggc ctcgttcgac atcaccgtcg acgaccccga cgactgacct gttcaagcca
     3361 ttgatctcgt cgtcatggcc gcgagatcac cggccagaac gggtcagggg gtgaggaagc
     3421 cgcgttcggt gaggaggtcg atgatgcgct cgacgttgtc ggcctggtcg acggtggcgg
     3481 tgtcgagggt caggtccggg ttctcgggga cgtcgtaggt cgcggagacg ccggggaact
     3541 gcgggatctc gccccggtcc gcagcctcgt agaggccctc gggatcccgc tggcgggcga
     3601 cctcgaccgg ggcgtcgagg tggacgatca ggaagcgctc ggggccgacc agctcccggg
     3661 cccggtcgcg gacgtcggcc ttgggggcgg caaaggcggc gatcacgatg atcccggact
     3721 cgttggccag gcgggccacc tcggccatgc ggcgcaggtt ctcggagcgt tcctgggcgc
     3781 tgaagccgag gtcgcggctc attcccagac gcaggtcctc gccgtcgatg cgcaacgtcg
     3841 ctcggccggc gtcgaacagg cggcgctcga gggccaccgc cgtcgtcgac ttcccggccc
     3901 ccgtgagccc ggtcagcagg acggtgcacg ggcgctggcc gtagcggctc tcgcgctccg
     3961 ccaccgtgat ctggctcagg tggcgctgca ggtgctcgtc gggctggacg tcccaggccg
     4021 agtcctgggg caggatcatg cccgccgcca tcgtggcgtt gctcaggcgg tcgaccagga
     4081 tgaacgcgcc cgtcgtgcgg ttgcgctcgt aggcgtcgaa gagcagctgc tggtcgcagg
     4141 tgaccgtgca gcgcccgatc tcgttgacgg ccaggccggc ggcggcctcg ggctcgaggg
     4201 tgttgatgtc gatgcggtgg gccatcgtgg tgatgcggcc cgggacgatc cggttggcct
     4261 gctggaccag gtactccttg cccggggcca tgtcggtctc gctcatccac acgaccatgg
     4321 cgtcgacctc gtggctgcgt tgcggccggc gctcggggtg gacgagcacg tcgccccggg
     4381 acacgtcgac ctcgtcggtc aaggtcatgg tgagggcggt gccggggccg gcctcgtcga
     4441 ggtcgccgtt gaacgtgacc aggcgatcca cggtcgtgac cacgccggag ggcaggacca
     4501 tgacctcgtc gccggggtgg acgacaccgg aggcgacggt gccggcgaag ccccgaaagg
     4561 tcaggtccgg gcgccgcacc agctggaccg ggaagcgcag gtcgccgtcc tcgggcaccg
     4621 tgaccggcac ggtctcgagg tgctccatga gggtcgggcc gtcgaaccag ctcatgttcg
     4681 tgccggggtc gaccacccag tcgcccttca gggcggagat ggggatgaac cacggcgcct
     4741 tcttcaggtc gaggcgctcg gcgaactcga ggtagtggtc ccggatctcc tcgaagcgct
     4801 ccttcgagtg gtcgacgagg tccatcttgt tgatggccac gaccgtgttc tcgatgccga
     4861 ggagcgaggt caggtagctg tggcgcttgg tctgggcctg cacgccgtgc cgggcgtcca
     4921 ccaggatgat ggccagatcg caggtcgagg ctcccgtgac catgttgcgg gtgtactgct
     4981 cgtggcccgg ggtgtcggcg atgatgaact tgcgccgcga ggtcgagaag tagcggtagg
     5041 cgacgtcgat ggtgatgccc tgctcccgct cggccttcaa gccgtccatg agcagggcca
     5101 ggtccagctc accgccggcg gacccgatgg tcgtcgactc ggcttccacc gcggccaact
     5161 ggtcctcgta gagcagatgg gagtcgtgca ggaggcggcc gatcagggtc gacttgccgt
     5221 cgtccaccga cccgcaggtc aggaagcgga gcaggtcctt ctgttcgtgc tcggccaggt
     5281 acgcctcgat gtcggtggcg atcagctcgg actggtgcga catctagaag tacccctcct
     5341 tcttcttctc ctccatggag ccggcctggt cgtggtcgat gacccggccc tcccgctcgg
     5401 agcgagtggc caacagcatc tcctggatga tctgggggag ggtggtggcg gtggactcga
     5461 cggcgccgga gagcgggtag cagcccaggg tgcggaagcg gatcatccgg tcgacgggct
     5521 cctcgccggg ctcgaagcgg aagcggtcgt cgtcgaccat gatcagcgtc ccgtcccgct
     5581 cgaccgtcgg gcggagggcc gagtagtaga gcggcaccag cgggatctcc tcgagccaga
     5641 tgtactgcca gatgtcgagc tcggtccagt tcgacagcgg gaagacccgg atgctctcac
     5701 cggggtggac ccgcccgttg tagaagttcc acagctcggg gcgttggctc ttggggtccc
     5761 agcggtggtg cttgtcccgg aacgagaaga cccgttcctt ggcccgggac ttctcctcgt
     5821 cgcgacgggc gccaccgaag gcggcgtcgt agcccccggc gtccagggcc tgcttgaggg
     5881 cctgggtctt catgatgtcg gtgtagttct tggagccgtg gtcgaagggg ttgatgccgg
     5941 cggccacgcc gtcctggttc gagtggacga tcagctcgac gcccagctcc tcggccattc
     6001 ggttgcggtg ggcgatcatg tccttgaact tccacgtggt gtccacgtgc agcaggggga
     6061 acggcagctt gccggggtgg aaggccttct cggccaaccg gagcatgacc ccggagtcct
     6121 tgccgatgct gtagagcatc accggccgtt cgaactgagc caccacctca cggatgatgt
     6181 ggatgctctc gtcttcgagc tgctggaggt gcgtgcggcg gggcgcgtca gggagggcgt
     6241 ctgcggtcat ggaaggggtc atcttcccac ggggtccttt tccgggccag ttggcgtgta
     6301 atgggcgccg cccttgcggc tgcgcttgga gaggcggtca cggatgaggc ggcggcgcgc
     6361 ctggagctcg gccagcgtga tggcttcggc gtcgggcatg ccgagcgagg cccggtagcc
     6421 gtccaagctc acctcggcca ggtaggcgtc ggggtcgtac tcctgctgga ggtcggcggc
     6481 gatgcgctcg ccgtgggtgg tcgcgaagtg gacgatcacc tgctggatca gctcgaggat
     6541 gtccaggtcg ggggccaggg tctggcccat cgaggtgctg aaggccatgt tcttgacgaa
     6601 cagcatcagc tccttgggca tgcgggcgcc gtagccgagc agcgccttga gcacccgctg
     6661 cagctcgccc accatctcgt cagggtccat ctgggtgggg tcgacggcgg gggcgtcgag
     6721 gccgagctcg gcgatgaggg cctcgaagtc ggtgtccagc ggcagggcgc cgagctcggc
     6781 cagggcgggg agctggaccc ggacgtcatt ggtgctgccg ccggcgagca gcttgaggaa
     6841 ggccaggcgc cgaggttcgt cgagccgacc ggtgatgccg aagtcgagca gcgcggtgcg
     6901 gccgtcgggt tggacgaaga ggttgccgcc gtgcaggtcg ccgtggaaga cgccgtggaa
     6961 catggctccc tcgaggaagc cgatgatccc ggtgcggatg agggccatca ggtcgacgcc
     7021 ggccgccttc atggcatcga cctcgtcgag ggcgaagccc gacaggcgct ccatcaccag
     7081 gacc
//
LOCUS       DASTFV010000629         3040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2393520, whole genome shotgun sequence.
ACCESSION   DASTFV010000629 DASTFV010000000
VERSION     DASTFV010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2393520"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VFP02_11530"
     CDS             <1..303
                     /locus_tag="VFP02_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543710.1"
                     /translation="EAIVCDAPSNVERRDYAAIEHAAWDDRHELGDFPVAVISNDYGS
                     DATEPGERTNAQAQRGWLVLSPNSHQVIVTSGHDVPVNEPDLVVDEILGVLEDARG"
     gene            complement(522..2012)
                     /locus_tag="VFP02_11535"
     CDS             complement(522..2012)
                     /locus_tag="VFP02_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543711.1"
                     /translation="MTNDDHLTDLLAERASQGSRVRPTMDGVQDAIARRARRRHRQRL
                     VGTGLAALCVVAGIAGGFALAGGDDTEAPATADLTEVPLVGVDQPGLELVETHVGPLG
                     AGTDPPLSYTSFASSVEPLGPLMTVMVDPAGDVSPPAGRAVDLDGDGHAAGEGDGFVT
                     VGTGGVGLLWPLPDGRVAGFGAFELSEAFLVDYVQGLDREAFDVAALPTPDGLDQRRT
                     STLPSPVATQALSAYTGDGGTLQVLTTNDPGWFDFMRMTIDDFMRMTIDANITGNLTE
                     TPLGQSFLGPGVAESTPAGEGSAFAVVRTESGLTIEIWTEGIGVDVVNQILAEGRFVE
                     VAPTEAPTTTTTVEAVTTTTEVPAPTTAVADAGSTLITDITIEPGDELDRLELFFAGT
                     VPEDYELTPAEEAVDVDCHGPVTDGGDYLVLRLGAPAGSGLDDWDGVLQITPDPNGAL
                     QGVLSCGWSDGDAYISLDLDQVREAEIYQGIGQPVLIIDILHPLDR"
     gene            complement(2009..2506)
                     /locus_tag="VFP02_11540"
     CDS             complement(2009..2506)
                     /locus_tag="VFP02_11540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HET9543712.1"
                     /translation="MKAVAEQIDPTPPAEPAYVETTFEALYADEVDAMARLAFLMVGS
                     EEQAEELVHDAFARLYERWDRVDNPGGYLRTCVVNGCKDRLRRRGVERRHPTASSGAA
                     ELEADHLADVLAALPYRQRAAVVLRYYEDRSEADIADLLGVRPGTVKSLLHRGLATLR
                     REIEP"
     gene            2634..>3040
                     /locus_tag="VFP02_11545"
     CDS             2634..>3040
                     /locus_tag="VFP02_11545"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9543713.1"
                     /translation="MRKLVVSEFVSLDGVMEAPGGEDGYPHGAWTVPYWGDDLGEFKT
                     AELEAADALLLGRVQYEGFAGAWPERGGDPFSDKMNSMAKFVVSSTLEDPAWENTQVL
                     EGDPLEAVAALKAEEGDDILVAGSSTLAKSLLRA"
BASE COUNT          454 a         1025 c         1108 g          453 t
ORIGIN      
        1 gaggcgatcg tctgtgacgc cccgagcaac gtcgagcgcc gcgactacgc cgccatcgag
       61 cacgccgcgt gggacgaccg gcatgagctc ggcgatttcc ccgtcgccgt gatctcgaac
      121 gactacggct cggacgccac cgagcccggt gagcggacca atgcgcaggc tcagaggggc
      181 tggctcgtcc tcagcccgaa ctcccaccag gtgatcgtga cctccggcca cgacgtgccg
      241 gtgaacgagc ccgacctcgt cgtggacgag atcctgggtg tgctcgagga cgctcggggc
      301 tgaccagtat cagtcggcgg ggatgtcgat gacgatcacc gatcgctccg gcgcgcgccg
      361 ggcttccgac gagccgagac atggccttcg cctcggtgac gtagagcgcc tcgaaggacc
      421 ccgtccacgt agggcgcatc gacatccttc gtggcggccc tcacaccttc aacgacgagt
      481 gagccgccga tgaggctgca ccggccgact ctaccgaccg gtcatcggtc caacgggtgg
      541 aggatgtcga tgatgaggac cggttggccg atgccctggt agatctcggc ctcccggacc
      601 tgatccaggt cgagggagat gtaggcgtcg ccgtcggacc aaccgcagct gagcaccccc
      661 tgcagcgccc cgttcgggtc gggggtgatc tgcagcaccc cgtcccagtc atcgagccca
      721 ctacccgccg gggcgccgag gcgcaggacc aggtagtccc cgccgtcggt gacgggaccg
      781 tgacagtcga cgtcaacagc ctcctccgcc ggggtcagct cgtagtcctc cggcaccgtc
      841 ccggcgaaga agagctcgag ccgatcgagc tcgtcgcccg gctcgatcgt gatgtcggtg
      901 atcagggtgc tgccggcgtc ggcgacagcc gtcgtcggag ccgggacctc ggtcgtcgtc
      961 gtgaccgctt cgaccgtcgt ggtggttgtc ggcgcctccg tcggcgcgac ctccacgaag
     1021 cggccctcgg cgaggatctg gttcacgacg tcgacgccga tgccctcggt ccagatctcg
     1081 atggtgaggc cggactcggt gcggacgacg gcgaaggccg acccctcacc ggcgggggtg
     1141 ctctcggcga ctcccgggcc gaggaacgac tgaccgagcg gagtctcggt caggttgcca
     1201 gtgatgttcg cgtcgatcgt catgcgcatg aagtcgtcga tcgtcatgcg catgaagtcg
     1261 aaccacccgg ggtcgttggt ggtcaagacc tgcagggtcc cgccgtcgcc ggtgtatgcg
     1321 ctcagtgcct gggtggcgac gggcgacggg agggtcgacg tgcgccgctg gtcgagaccg
     1381 tcgggggtgg gcagcgccgc cacgtcgaag gcctcccggt cgagcccctg cacgtagtcg
     1441 acgaggaacg cttcggacag ctcaaaggcc ccgaacccgg ccacgcggcc gtccggcagt
     1501 ggccacagca gtccgacgcc acccgtgccg accgtgacga agccgtcgcc ctcgcctgcg
     1561 gcgtggccgt caccgtcgag gtcgacggct cggccggcgg gcggggacac gtcgccggcc
     1621 gggtccacca tcacggtcat cagcggcccc agcggctcga cggaactggc gaaggaggtg
     1681 tagctgaggg gcgggtcggt gccggcgccg aggggaccca cgtgggtctc gaccaactcg
     1741 aggcccggct ggtcgacgcc gaccaggggc acctccgtca gatcggccgt ggccggcgcc
     1801 tcggtgtcgt ccccgcccgc caaggcgaag ccgcccgcga tgccggccac gacgcacagg
     1861 gcggcgagtc cggtgccgac gaggcgctgg cgatgacgcc ggcgggcgcg gcgggcgatg
     1921 gcgtcctgga cgccgtccat cgtggggcgg acccggctcc cctggctcgc tcgctcggcc
     1981 agcaggtcgg tcaggtggtc gtcgttggtc atggctcgat ctccctacgg agggtggcga
     2041 ggccgcggtg caggagggac ttcaccgttc ccgggcgcac gcccagcagg tcggcgatgt
     2101 ccgcttccga tcggtcttcg tagtagcgga ggacgacggc ggcccgctgt cggtacggca
     2161 acgcggcgag gacgtcggcc aggtgatcag cctcgagctc ggcggcaccc gaggaggccg
     2221 tcgggtggcg gcgctcgacg ccccgtcgac ggaggcggtc cttgcagccg ttcaccacgc
     2281 aggtccggag gtagcccccg gggttgtcga cccggtccca gcgctcgtag aggcgggcga
     2341 aggcgtcgtg gaccagttcc tcggcctgct cctcggaccc gaccatcagg aacgccaggc
     2401 gggccatggc gtcgacctcg tcggcgtaga gcgcctcgaa ggtcgtctcg acgtaggccg
     2461 gctcggccgg cggagtgggg tcgatctgtt cggccactgc cttcacgtcc tcaacgacgc
     2521 ccaggtcggg taggtggttg ccagaaatct gccatggcct gtcgaaaccg gccgagtgcg
     2581 ttcgtggaca tcatggagga cgggaacagc ctgtcccgat cggaggagga accatgcgca
     2641 agctggtcgt gagtgagttc gtgtccttgg acggcgtgat ggaggccccc ggcggcgagg
     2701 acggctaccc gcacggggcg tggaccgttc cctactgggg cgacgacctc ggcgagttca
     2761 agaccgcgga gctggaggcg gccgatgcgc tgttgctcgg acgggtgcag tacgagggct
     2821 tcgccggagc ctggcccgag cggggcggcg atcccttctc cgacaagatg aactcgatgg
     2881 ccaagttcgt ggtctccagc acgctcgagg acccggcatg ggagaacacg caggtgctcg
     2941 agggtgatcc cctcgaggct gtcgccgcac tgaaggccga ggagggcgac gacatcctcg
     3001 tcgccgggag ctccaccctg gccaagtccc tgctccgggc
//
LOCUS       DASTFV010000630         3310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2396930, whole genome shotgun sequence.
ACCESSION   DASTFV010000630 DASTFV010000000
VERSION     DASTFV010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2396930"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFP02_11550"
     CDS             complement(<1..477)
                     /locus_tag="VFP02_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376950.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET9543714.1"
                     /translation="MSPRYVVRTFGCQMNEHDSERIAGLLEGDGMVPADDLDDADVIV
                     LNTCCIRENADNKLYGHLGHLKALKDERPDLQIAVAGCLAQKDRETIRERAPHVDVVF
                     GTHNVHRAADLLTQAREQGPIVEILEATVLEDAHAFPSVLPASRESQHSAWVTIQIG"
     gene            complement(737..1819)
                     /gene="recA"
                     /locus_tag="VFP02_11555"
     CDS             complement(737..1819)
                     /gene="recA"
                     /locus_tag="VFP02_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET9543715.1"
                     /translation="MKGTTVERDKALEMAMAQISKAHGEGAVMKMSDRAAMKVEVVST
                     GALALDIALGIGGLPRGRVTEIFGPESSGKTTLAMHVVAEAQRNGGICAFIDAEHALD
                     PTYAAAIGVDVDELLISQPDTGEQALEITDMLIRSGAIDVVVIDSVAALTPRAEIEGE
                     MGDTHVGLQARLMSQALRKLTANLNKTNTICIFINQLREKIGVMFGSPETTPGGRALK
                     FYSSARLDIRRIEAIKDGVEVVGNRTRVKVVKNKCAAPFKQAEFDIMYGRGISREGSV
                     LDIGVDLGFIKKSGAWYTYEGEQLGQGRENAKEFLTENAEVMVEVSERIRTEVGLGDA
                     IDLSDGEAIDDEDESMDEDELLSLDD"
     gene            1990..2427
                     /gene="msrB"
                     /locus_tag="VFP02_11560"
     CDS             1990..2427
                     /gene="msrB"
                     /locus_tag="VFP02_11560"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET9543716.1"
                     /translation="MSEPTLTDAEYREKLTPEQYHVARQGGTERAFTGIYWDEHDDGV
                     YRCICCDAPLFSSGTKFESGSGWPSFYEALDPDAVELISDRSLFMTRTEVRCANCDAH
                     LGHVFDDGPAPTGQRFCMNSASLRLDRDAAADEDDTEDEAAEA"
     gene            2468..2935
                     /locus_tag="VFP02_11565"
     CDS             2468..2935
                     /locus_tag="VFP02_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543717.1"
                     /translation="MIALLGVALVVLGTFLPIVEYDLGSIGAGIEDSVNGWSDAINDG
                     PIHVVVGLVPLVMGILALMNRARVAVKVLLIVSAVIGFFWVFVRFADISGSLEEGDAS
                     AFVAEVADPGVGLYVMTVGWVLVLVAAIVATARKPRPVPAAPYAQAYLSPSGV"
     gene            complement(2911..>3310)
                     /locus_tag="VFP02_11570"
     CDS             complement(2911..>3310)
                     /locus_tag="VFP02_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543718.1"
                     /translation="SADIDEVTSALAGVRHPPVEAVVGPATELLGEGVLHLPVAGLGS
                     LADGVVAATRDLGRPPEGQPFLGHLTLARMAGVPREGVLDQAFSARFAVGAVHLVSSS
                     LGGDAARYETVATVPLSRGTVPPQTPEGDR"
BASE COUNT          477 a         1148 c         1142 g          543 t
ORIGIN      
        1 gccgatctga atggtgaccc aggccgagtg ctggctctcc cggctggcgg gcagcacgga
       61 ggggaaggcg tgggcgtcct cgaggacggt ggcctcgagg atctccacga tcgggccttg
      121 ctcccgagcc tgggtgagca ggtcagcggc ccggtgcacg ttgtgggtac cgaagacgac
      181 gtcgacgtgg ggggcccgct cccggatggt ttcccggtcc ttctgggcca ggcagccggc
      241 gaccgcgatc tgcaggtcag ggcgctcgtc cttcagggcc ttgaggtgcc cgaggtggcc
      301 gtagagcttg ttgtcggcgt tctcgcggat gcagcaggtg ttgagcacga tgacatcggc
      361 gtcgtcgagg tcatcggccg ggaccatgcc gtcgccctcc agcaacccgg cgatgcgctc
      421 ggagtcgtgt tcgttcatct gacacccgaa ggtgcgcacg acataccgcg ggctcacggg
      481 atcagcctag ggagcagcag ggggctcggc tggtcctcca cggtctccaa ccccctccag
      541 gcggggcccg gtcggcggcg cactgcccga cgaagccacc accaccgccg aggcgttcgg
      601 cttcgacggc atcacccgga ccgccgccgc cgtcacctcg ccctgacacc gccccgaccg
      661 gcgacgcgcc gcggccgccg tccgggggga tggaacggcg gccgcgtggt gctgcccggc
      721 gggtcggcga agggtgtcag tcgtcgaggc tgaggagctc gtcctcgtcc atggactcgt
      781 cctcgtcgtc gatggcctcg ccgtcggaga ggtcgatggc gtcacccagg ccgacctcgg
      841 tccggatgcg ctcggagacc tcgaccatga cctcagcgtt ctcggtcagg aactccttgg
      901 cgttctcccg gccctggccg agctgctcgc cctcgtaggt gtaccaagcg cccgacttct
      961 tgatgaaccc gaggtccacc ccgatgtcga ggaccgagcc ctcccggctg atgccccggc
     1021 cgtacatgat gtcgaactcg gcctgcttga acggggccgc acacttgttc ttcaccacct
     1081 tgacccgggt gcggttgccc accacctcga cgccgtcctt gatggcctcg atccgccgga
     1141 tgtccaaccg cgccgaggag tagaacttga gtgcacgacc acccggagtg gtctccgggc
     1201 tgccgaacat gacgccgatc ttctcccgca gctggttgat gaagatgcag atggtgttgg
     1261 tcttgttcag gttggccgtg agcttgcgca gggcctggga catcagccgg gcctggaggc
     1321 cgacgtgggt gtcgcccatc tcgccctcga tctcggcccg gggcgtcagg gcggcgaccg
     1381 agtcgatgac gaccacgtcg atggcgccgg agcggatcag catgtcggtg atctccaacg
     1441 cctgctcacc cgtgtcgggc tgggagatga gcagctcgtc cacgtccacc ccgatggcgg
     1501 cggcgtaggt cgggtcgagg gcgtgctcgg cgtcgatgaa ggcacagatg cccccgttgc
     1561 gctgggcctc ggccaccacg tgcatggcca gggtggtctt gcccgaggac tccgggccga
     1621 agatctcggt gacccggccg cggggcagac cgccgatgcc gagggcgatg tccagcgcca
     1681 gggcgccggt ggagaccact tcgaccttca tggcggcgcg gtcgctcatc ttcatgaccg
     1741 ccccctcgcc gtgggccttg gagatctggg ccatggccat ctcgagggcc ttgtctcgtt
     1801 ccacggtggt tcctttcatc ggagtggctt gactgatggt caacctacga agggggtgtg
     1861 acactcatgc cgggaacccc gtggacctca ccggggttgt agagcatgac ggcccaacca
     1921 gtgcgaacga caggaatgta gttccgaacg gctgttcgat caagtctttc gtcgagaaaa
     1981 aggacgacca tgagcgagcc caccctcacc gacgccgagt accgggagaa gttgacgccg
     2041 gagcagtacc acgtcgcccg ccagggcggc accgagcggg ccttcaccgg catctactgg
     2101 gacgagcacg acgacggcgt ctaccgctgc atctgctgtg acgcccccct gttctcctcc
     2161 ggcaccaagt tcgagtccgg ctccggctgg cccagcttct acgaggctct cgaccccgat
     2221 gccgtcgagt tgatcagtga ccgcagcctc ttcatgaccc gcaccgaggt ccgctgcgcc
     2281 aactgcgatg cccatctggg ccatgtcttc gacgacggcc cggcccccac cggccagcgg
     2341 ttctgcatga actcggcgtc gctccgcctc gaccgcgacg ccgcggccga cgaggacgac
     2401 accgaggacg aggccgccga ggcgtgatcg acgccgggac ggccccgcgc cggggccggc
     2461 cgcgggggtg atcgctctcc tcggcgtggc gttggtggtc ctggggacct tcctccccat
     2521 cgtggagtac gacctgggca gcatcggtgc tgggatcgag gattccgtca acgggtggag
     2581 cgacgcgatc aacgacgggc cgatccacgt cgtcgtgggc ctggtcccgc tggtgatggg
     2641 gatcctcgcg ctgatgaacc gggctcgagt cgcggtcaag gtcctgctga tcgtgtccgc
     2701 cgtgatcggg ttcttctggg tgttcgtccg cttcgccgac atctccggtt cgctggagga
     2761 gggcgacgcg agcgccttcg tcgccgaggt ggcggacccg ggtgtcgggc tctacgtcat
     2821 gacggtcggc tgggtgctcg tcctggtcgc cgccatcgtg gccaccgcca ggaagccccg
     2881 cccggtgccc gccgcgccct acgcccaggc ctacctgtca ccctccggcg tctgaggagg
     2941 gaccgttccg cggctgagtg gaaccgtggc caccgtttcg tagcgcgccg cgtcgccgcc
     3001 caacgagctg gacaccaggt ggacggcgcc gaccgcgaaa cgggcggaga aagcctgatc
     3061 caggacgccc tcgcgcggca cgcccgccat ccgggccagg gtgaggtggc cgaggaacgg
     3121 ctggccctcc ggcggccgac ccaggtctcg ggtggcggcg accacgccgt cggccaggga
     3181 gcccagcccg gccaccggca ggtgcagcac cccctccccc agcagctccg tcgccggacc
     3241 gaccacggcc tcgacgggcg ggtgccgtac accggccagg gcactcgtca cctcgtcgat
     3301 gtcggccgac
//
LOCUS       DASTFV010000631         4122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2399042, whole genome shotgun sequence.
ACCESSION   DASTFV010000631 DASTFV010000000
VERSION     DASTFV010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2399042"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /gene="folE"
                     /locus_tag="VFP02_11575"
     CDS             complement(<1..429)
                     /gene="folE"
                     /locus_tag="VFP02_11575"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HET9543719.1"
                     /translation="MSEEGGKNHAVPPVDKPRIEAAMREILAAIGEDPDRDGLQQTPA
                     RVARMYEEIFAGLREMPDHHLQVTFDASHDEMVMVRDIALVSTCEHHLVPFMGKAHVA
                     YIPNDDGRITGLSKLARLVDAYAKRPQVQERLTTQIADEIE"
     gene            complement(426..1007)
                     /gene="hpt"
                     /locus_tag="VFP02_11580"
     CDS             complement(426..1007)
                     /gene="hpt"
                     /locus_tag="VFP02_11580"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET9543720.1"
                     /translation="MSDPPAGDDDGSVVRVDDPHLGRILVPADRLAARVAELGVQITA
                     DYQGRPPLLVGVLKGAFMFMSDLSRHIALPVEFDFMAVSSYGAATRTSGVVRIVKDLD
                     IDLSGRDVLLVEDIVDSGLTLRYLKRNLESRNPASLEVCALLLREGAADGASLKYVGF
                     EIPPDFVIGYGLDVAEKYRNLPYICAYNEELPP"
     gene            complement(1018..1890)
                     /gene="tilS"
                     /locus_tag="VFP02_11585"
     CDS             complement(1018..1890)
                     /gene="tilS"
                     /locus_tag="VFP02_11585"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET9543721.1"
                     /translation="MGLRDDLLARCRFPEPGSEVSCAVSGGADSLSLLLLAVAAGCRA
                     TAIHVDHGLRHDSEREADRVAAAAARYGARFRSERVTVLPGANLQARARAARYGVLPA
                     DVLTGHTADDQAETMVLNLLRGAGRPGLAGMRRTQRRPLLDLRRAETAALCVAEGLDP
                     LVDPTNDDLTHRRTRVRHEVLPLLDDVAGRSVVPILARQAEILGEETEYLDRMADALD
                     PTDTRALQAAEPALVRRALRAWLWRSMGADHPVDAAAIDRVLAVVRHDSRAADVQGGW
                     RVVRTEGRLTLERA"
     gene            complement(1898..2962)
                     /locus_tag="VFP02_11590"
     CDS             complement(1898..2962)
                     /locus_tag="VFP02_11590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HET9543722.1"
                     /translation="MTAVIDWDLAERVAARFSGREPFADSYHYDALGPDFAELTAIAE
                     DRVARATGLVSTAGPARARVVDRPAWIAANLASFQRLLRPLTDKLGERMGTSPLAPVA
                     QKIAGAEVGALLGWMSTRVLGQYDLLVVEDEDLENQDIVYYVGPNVLALEKRFAFPPR
                     EFRLWLALHEVTHRAQFTGIPWLRTHYLGLVDEVVGAVDPDPKRFIAAMARVADDLRE
                     GRKPLDGGGLAAVLANPEQRLALDRVAGLMSLLEGHGDVTMDRAGADQIPSADRFGRV
                     LRQRRQQTNGLARLLQRLMGLEAKLAQYEQGEKFIEAIEAVGGPTALDAVWEDPARLP
                     TLAEIRDPAGWLARIDLAAA"
     gene            complement(2981..3109)
                     /locus_tag="VFP02_11595"
     CDS             complement(2981..3109)
                     /locus_tag="VFP02_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543723.1"
                     /translation="MVLGVVWHFWLAPVLVLTVLAIAVMTVFGYLRKVSAPRYPKN"
     gene            3146..4105
                     /locus_tag="VFP02_11600"
     CDS             3146..4105
                     /locus_tag="VFP02_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543724.1"
                     /translation="MRRIGGITVVVFILAGACGHDDGVDAGADDTADTTEPTVTAVTP
                     VPTRTEVERLTLDLVDPSRPTPAGAGLPHADDRRLVTDVYVPAGTGPFPFIAFSHGLA
                     GHPRKFTQLLGQWAEAGYAVAAPAFPLSNDEVPGVSTFADLPSQPGDVSFVIDRLLAM
                     SDDEDDPLYGVVDPDRIGVAGLSLGGATTYGVAFNDCCADERPMAAMVLDGARLAVGG
                     EFHMDSGLPLMIIHADEDFALPYAEAADAYVDAIAPKYLVTLHEFAHASPYENDPDPA
                     DELVTASTIAFWDRYLKDDESAEQRIVDAITPSSLATLESDTG"
BASE COUNT          581 a         1483 c         1491 g          567 t
ORIGIN      
        1 ctcgatctcg tcggcgatct gggtggtcag ccgttcctgg acctgtggcc gcttggcgta
       61 ggcgtcgacc aggcgggcca gcttggacag gccggtgatg cggccgtcgt cgttggggat
      121 gtaggcgacg tgggccttgc ccatgaaggg gacgaggtgg tgctcacagg tcgacaccag
      181 ggcgatgtcc ctgaccatga ccatctcgtc atggctggcg tcgaaggtga cctggaggtg
      241 gtggtcgggc atctcgcgga ggccggcgaa gatctcctcg tacatgcggg ccacccgagc
      301 cggtgtctgc tgcaggccgt cccggtcggg gtcctcgccg atggcggcca ggatctcgcg
      361 catggcggcc tcgatgcggg gtttgtcgac tggcgggacg gcgtggttct tccccccctc
      421 ctcgctcacg gggggagctc ttcgttgtag gcgcagatgt acggcaggtt gcggtacttc
      481 tcggccacgt cgaggccgta gccgatgacg aagtccggcg ggatctcgaa gcccacgtac
      541 ttgaggctgg ccccgtcggc cgccccctcc cgcaggagca gggcgcacac ctcgaggctg
      601 gccgggttgc gggactcgag gttgcgcttg aggtagcgca gggtcaggcc gctgtccacg
      661 atgtcctcga ccagcaggac gtcgcgcccc gacaggtcga tgtcgaggtc cttcacgatc
      721 cggaccacgc ccgacgtgcg ggtggcggcg ccgtaggagg acacggccat gaagtcgaac
      781 tcgacgggca gggcgatgtg gcgggacagg tcgctcatga acatgaacgc ccccttcagc
      841 acccccacca ggagcggtgg tcggccctgg tagtccgcgg tgatctgaac ccccagttcg
      901 gccacccgcg ccgccagccg gtcggcgggc accaggatcc gcccgagatg cggatcgtcg
      961 acccgcacca cggacccgtc gtcgtctccg gccggggggt cgctcacctc ggggaatcta
     1021 cgcgcgctcc agcgtcaaac gcccttcggt gcgcaccacc cgccagcccc cctggacgtc
     1081 ggcggcccgg gagtcgtgcc ggacgacagc caggacgcgg tcgatcgcgg cggcgtcgac
     1141 cgggtggtcg gcccccatcg agcgccagag ccaggcccgc agggcccggc gcaccagggc
     1201 cggctcggcg gcctgcaggg ctcgggtgtc ggtggggtcc agggcgtccg ccatgcggtc
     1261 caggtactcg gtctcctcgc ccaggatctc ggcctggcgg gccaggatgg gcacgacgga
     1321 tcggccggcc acgtcgtcga ggaggggcag cacctcgtgg cgcacccggg tccggcggtg
     1381 ggtgaggtcg tcgttggtgg gatcgaccag cggatccagg ccctccgcca cgcagagggc
     1441 cgcggtctcg gcccggcgca ggtcgagcag cgggcggcgc tgggtgcggc gcatgccggc
     1501 cagccccggg cgcccggccc cccgcaggag gttcagcacc atcgtctcgg cctggtcgtc
     1561 ggccgtgtgg cccgtgagca cgtcggcggg caggaccccg tagcgggccg cccgggcccg
     1621 ggcctggagg ttcgcgccgg gcagcacggt gacccgctcg gaacggaagc gagcgccgta
     1681 gcgggccgcc gcggccgcca cccggtcggc ctcccgttcc gagtcgtgcc ggaggccgtg
     1741 gtcgacgtga atggcggtgg ctcgacaacc tgcggccacg gccagcagga gcagggagag
     1801 cgagtcggcc ccgccggaga ccgcgcaact cacctcggac cccggttccg ggaaccggca
     1861 gcgggccagc aggtcgtccc ggaggcccat ggcggcgcta cgcagcggcc aggtcgatcc
     1921 gggccagcca cccggcgggg tcccggatct cggccagggt cggcaggcgc gccgggtcct
     1981 cccacaccgc gtccagggcc gtcgggccgc ccacggcctc gatggcttcg atgaacttct
     2041 caccctgctc gtactgggcc agcttggcct cgaggcccat caggcgctgg agcaggcggg
     2101 ccaggccgtt ggtctgctgg cgccgttggc gcaggacccg tccgaagcgg tcggccgaag
     2161 ggatctggtc ggccccggcc cggtccatgg tcacgtcgcc gtggccctcc agcaggctca
     2221 tgaggcccgc gacccggtcg agggcgaggc gctgctccgg attggccagc acggcggcca
     2281 ggccgccgcc gtcgaggggc ttgcgccctt cccggaggtc gtcggccacc cgggccatcg
     2341 cggcgatgaa gcgcttgggg tccgggtcga ccgcgccgac gacctcgtcc accagcccga
     2401 ggtagtgggt gcgcagccat gggatgccgg tgaactgggc ccggtgggtc acctcgtgca
     2461 gggccagcca gagacggaac tctcgcggcg ggaaggcgaa gcgcttctcg agggcgagga
     2521 cgttggggcc cacgtagtag acgatgtcct ggttctcgag gtcctcgtcc tcgaccacca
     2581 gcaggtcgta ctggccgagg acccgggtcg acatccagcc cagcagggcg ccgacctcgg
     2641 ccccggcgat cttctgggcg accggcgcca gcggactggt ccccatccgc tcgcccaact
     2701 tgtcggtcag gggccgcagc aggcgttgga acgaggccag gttggcggcg atccaggccg
     2761 gtcggtcgac cacccgggcc cgagcggggc cggcggtgga gaccaggccg gtggccctgg
     2821 ccactcggtc ctcggcgatg gccgtgagct cggcgaaatc ggggcccagc gcgtcgtagt
     2881 ggtacgagtc ggcgaagggc tcccgacccg agaagcgagc ggcgacccgc tcggcgaggt
     2941 cccagtcgat gacggcggtc atcgagactc ggtcaccgga tcagttcttg gggtagcgcg
     3001 gggcggagac cttgcggagg tagccgaaga cggtcatcac cgcgatggcg aggaccgtca
     3061 gcacgagcac tggtgcgagc cagaaatgcc agacgacacc gagaaccatg ggcgcggagc
     3121 ataacgggta cgttctccca cccagatgcg ccggatcggg gggatcaccg ttgtcgtctt
     3181 catactggcc ggggcctgcg gccacgacga cggagtcgac gccggggccg acgacacggc
     3241 cgacacgacc gagccgacgg tcaccgcggt gaccccggtg ccgacgcgta cggaggtgga
     3301 gcgcctcacc ctcgacctgg tggacccgtc ccggcccacc cccgccggcg ccggtctgcc
     3361 ccacgccgac gaccgccggc tcgtgaccga cgtctacgtg cccgccggca ccggcccttt
     3421 tcccttcatc gccttcagcc acggcctggc cgggcacccc cgcaagttca cccagctcct
     3481 cggacagtgg gccgaagcgg gctacgccgt cgccgcgccg gcgttcccgc tgtccaacga
     3541 cgaggtgccg ggcgtctcca ccttcgccga cctgcccagc cagcccggcg acgtcagctt
     3601 cgtcatcgac cggctcctgg ccatgagcga cgacgaggac gaccccctct acggcgtcgt
     3661 cgacccggac cgcatcggcg tggccggcct gtcgctcggc ggcgccacga cctacggcgt
     3721 ggcgttcaac gactgctgcg ccgacgaacg gcccatggcc gccatggtgc tggacggcgc
     3781 ccgcctggcc gtcggcggtg agttccacat ggactcgggc ctgccgctga tgatcatcca
     3841 cgccgacgag gacttcgccc tcccctacgc cgaggcggcc gacgcctacg tcgacgccat
     3901 cgcgccgaag tacctggtga cgctgcacga gttcgcccac gcctcgccct acgagaacga
     3961 ccccgacccc gccgacgagc tggtcacggc cagcaccatc gccttctggg accgctacct
     4021 gaaggacgac gagagcgccg aacagcgcat cgtcgacgcc atcaccccgt ccagcctcgc
     4081 caccctcgaa tccgacaccg gctgacgacc gttctggcac cc
//
LOCUS       DASTFV010000632         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2405318, whole genome shotgun sequence.
ACCESSION   DASTFV010000632 DASTFV010000000
VERSION     DASTFV010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2405318"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..>2155
                     /locus_tag="VFP02_11605"
     CDS             81..>2155
                     /locus_tag="VFP02_11605"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET9543725.1"
                     /translation="MDPAPATVAARYVGTRVPRVEDERLLTGHGTFVDDIARPGMLHA
                     CFVRSPFARARVVGIDTSSALALDGVHAVFTAEDLHPDVHDPWYTIIGKEIPDTPRPP
                     MAEGEVRFVGDLVALVVAEDRYVAEDAAELVDVDYDPLPDVSDYVEAHGSGELVHQGY
                     EGNVAGYLRGRKPEAVDEACASAALVVTETIHQQAYVPVPMETRGIIVEWSGGELTIW
                     TGTQAPHEVRAFCSRLLGLDEHRIRVIMRDTGGGFGQKVNPLREDMCLMLAARKLPAA
                     VKWIEDRRENLLAAGQARHEHGEAKMAFAEDGALLAAYIDHVQDVGAYPTPWPVGTAA
                     AVGMIFPGPYRIPAAGWTTTSVFSNTAGRTAYRGPWQFESVAREMLLDIAARRMDVDP
                     IDLRRRNLLELEDLPYRNANGMTYDHISPSEVFELGLEMLDYEAFRREQAEAREAGRY
                     LGVGTCSYVEPTTTGMGYYGTEGATIRIEPSGKVNVYVAGGSTGNSLETTAVQLAADA
                     LGVDIEDVNTIQGDTAVTPFGLGTGGSRSGSMLAGAIGETAATLRERIVAIAAHKLEA
                     APEDIELSHGRATVRGTPAIGLTMEEIATIAYVDLASLPPDVPPGLEVSGRYRTQAPN
                     IWANATHVCTCEVDITTGVVTLLRYIVSEDCGPMINPSVVEGQIAGGTVQGIGGALYE
                     HLVYDEDGNP"
BASE COUNT          341 a          788 c          749 g          277 t
ORIGIN      
        1 gagaacggcc cctggggcct ggccggcttc accgagctcc aggtccagta ccgcgccaag
       61 ggctgacggc aaccgcagcc atggacccag cgccggccac ggtcgcggct cgctacgtgg
      121 ggacccgggt accccgggtc gaggacgagc gcctgctgac cggccacggg accttcgtcg
      181 acgacatcgc gcggccgggg atgctgcacg cctgcttcgt gcgcagcccc ttcgcccgcg
      241 cccgcgtcgt cggcatcgac acgtcgtcgg cgctcgcgct ggacggcgtg cacgccgtct
      301 tcacggccga ggacctccat cccgacgtcc acgatccctg gtacacgatc atcggcaagg
      361 agatccccga cacgccccgg ccgccgatgg ccgagggcga ggtccgcttc gtgggcgatc
      421 tggtggcgct ggtggtggcc gaggaccgct acgtcgccga ggacgcggcc gaactggtcg
      481 acgtcgacta cgacccactg cccgacgtgt ccgactacgt cgaggcccac ggatccggcg
      541 agctcgtgca ccagggctac gagggcaacg tcgcgggcta cctgcggggc cgcaagcccg
      601 aggcggtgga cgaggcgtgt gcgtcggcgg ccctcgtcgt caccgagacg atccaccagc
      661 aggcctacgt gccggtgccg atggagaccc gggggatcat cgtcgagtgg tcgggcgggg
      721 agctgacgat ctggaccggc acccaggcgc cccacgaggt gcgggccttc tgctcccggc
      781 tcctcgggct ggacgagcac cggatccgcg tgatcatgcg cgacaccggc ggtggcttcg
      841 gccagaaggt caacccgctc cgcgaggaca tgtgcctgat gctggcggcc cgcaagctgc
      901 cggccgcggt gaagtggatc gaggaccgac gcgagaacct cctggctgcg ggccaggccc
      961 ggcacgagca cggcgaggcc aagatggcct tcgccgagga cggcgccctg ctcgccgcgt
     1021 acatcgacca cgtgcaggac gtgggggcct acccgacccc atggcccgtc ggcacggcgg
     1081 ccgccgtcgg catgatcttc cccggcccct accggatccc cgccgcggga tggacgacga
     1141 cttcggtctt ctccaacacc gcggggcgca ccgcctaccg cgggccctgg cagttcgaat
     1201 cggtcgcccg cgagatgctg ctcgacatcg ccgcccgccg catggacgtc gacccgatcg
     1261 atctccgccg ccgcaacctg ctcgagctcg aggacctccc gtaccgcaac gccaacggga
     1321 tgacctacga ccacatctcg ccgagcgagg tcttcgagct cggcctggag atgctcgact
     1381 acgaggcctt ccgccgggag caggccgagg cccgggaggc cggccggtat ctcggggtcg
     1441 gcacctgcag ctacgtcgag ccgacgacga cgggcatggg gtactacggg accgaggggg
     1501 cgacgatccg gatcgagccc tccggcaagg tgaacgtgta cgtcgcgggg ggctcgaccg
     1561 gcaacagcct cgagaccacg gccgtgcagc tcgccgccga cgccctcggc gtcgacatcg
     1621 aggacgtgaa caccatccag ggcgacacgg cggtcacccc gttcggcctc ggcaccggcg
     1681 ggagccgcag tggctcgatg ctggcgggcg ccatcggcga gacggccgcc accctgcggg
     1741 aacgcatcgt cgccatcgcc gcccacaagc tcgaagccgc ccccgaggac atcgagctca
     1801 gtcatggccg ggccacggtg cgaggtacac cggcgatcgg actgacgatg gaggagatcg
     1861 ccacgatcgc ctacgtcgac ctggcgtcgc tgccgcccga cgtcccgccc gggctcgagg
     1921 tcagcggtcg gtaccggacg caggccccca acatctgggc caacgccacc cacgtctgca
     1981 cctgcgaggt cgacatcacg accggcgtgg tcaccctcct gcgctacatc gtgagtgaag
     2041 actgcggccc catgatcaac ccgagcgtgg tcgagggcca gatcgcgggc ggaacggtgc
     2101 agggcatcgg cggcgcgctc tacgagcacc tcgtctacga cgaggacggc aatcc
//
LOCUS       DASTFV010000633         3457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2406584, whole genome shotgun sequence.
ACCESSION   DASTFV010000633 DASTFV010000000
VERSION     DASTFV010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2406584"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VFP02_11610"
     CDS             <1..818
                     /locus_tag="VFP02_11610"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HET9543726.1"
                     /translation="SAAGDVVAVHVSGELPGGLWLVEVRRPVGAATEPMVLSGPQAVA
                     LADGGAVHLLAPFADSRRLWFARFELAGTVARHLEAHGRAIRYRYVGRDWPLETYQSV
                     FSTEPGSAEMPSAARPFTPEVVTDLVRRGVTFAPLLLHTGVSSLEGHETPYPEQYRVP
                     AATARLVNATHAAGARVVAIGTTVVRALETVTDRSGTTHPGEGWTEEVITPERGVRAV
                     DGLLTGWHEPEASHLLMLEAVAGRRPLELAYQAAYDNGYLWHEFGDSHLLLPS"
     gene            827..1477
                     /locus_tag="VFP02_11615"
     CDS             827..1477
                     /locus_tag="VFP02_11615"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HET9543727.1"
                     /translation="MPARTAPSLPEGRRNVLYAVRRRGEASAEQVAESLAMTVSGARQ
                     HLSALVDAGLAEATETEANGRPGRRTLVYSVTPTGDALFPKAYGELTNELLGFLDDEE
                     PETVNRLFARRRDARTANAQRRLAPLRTLADRVAELTRILDEDGYLASWEANADGSFL
                     IVEHNCAIWAVASRYGQACSSEIEFIQTVLDDARVERIQHMVEGARRCAYLVTPLG"
     gene            complement(1486..2787)
                     /locus_tag="VFP02_11620"
     CDS             complement(1486..2787)
                     /locus_tag="VFP02_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543728.1"
                     /translation="MATTVAPQSHTFERVASPPPVLMAMASLAAAAAVIHFVMVPSHM
                     DEFSVEGVAFAVAGWLQLLVAFYLWTQPSRAILGFTILSNLGFMAVWAVSRTVGLPVG
                     PHSGIAEEVSVVDLTCVALEAGLILMAIALLFRPRLAEVTLPPGLSVAVPLAIVVLAS
                     AVLASPEARNHAHGTDGVGATAVAGGGSAVSADGHAHGGAAAPAAAAAAVSDRGLSLL
                     TNGHHNAIVRQPMSPATQLELKRQLAITREVAAQYPTVADAEAAGYRRAGPYSPGLGT
                     HYVTQSGPALNFDGVVDDEDLRHPLSIIYDGSEPDSPVAGFMYYATTSTAPVGFASEN
                     DVWHYHEQICIKYVNGEIESPFGADLPVTDAQCSEVGGFMLDSTQYMVHVWSVPGYDN
                     VDAGVFAEVNPALDCADGSYWRLPSSEWATHPVNVCEAGAA"
     gene            complement(2879..>3457)
                     /locus_tag="VFP02_11625"
     CDS             complement(2879..>3457)
                     /locus_tag="VFP02_11625"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9543729.1"
                     /translation="AGRPVAVHCVTADALALLLAAWAEVGAWPTDRVEHGAVIPATAL
                     PALAALGVTVITQPGFVAERGDRYRAEVDEGEQPDLWRCATLLDGGVRVGAGTDAPYG
                     PADPWRAMAAAVNRRTPSGAIMGDDERLAPGRALGLFLGAADDPGGPPRRVEAGAAAD
                     LCVLDRPRVEALADLAAVGVTASFVAGVAVSV"
BASE COUNT          485 a         1260 c         1278 g          434 t
ORIGIN      
        1 agtcagccgc gggcgacgtc gtcgccgtcc acgtctccgg cgaactgccc ggcggcctgt
       61 ggctggtcga agtccgccga cccgtcgggg ccgccaccga gccgatggtc ctcagcgggc
      121 cgcaggcggt cgcgctggcc gatggtggcg cggtccacct cctcgccccc ttcgccgact
      181 cacggcggct ctggttcgcc cgcttcgagc tggccggcac cgtcgcccgc cacctcgagg
      241 cccacggccg ggccatccgc taccgctacg tcggccggga ctggcccctc gagacctacc
      301 agtcggtgtt ctcgaccgag cccggtagcg ccgagatgcc cagcgcggcc cgacccttca
      361 cgcccgaggt ggtcaccgac ctggtccgcc ggggcgtgac cttcgcaccc ctgctgctcc
      421 acaccggggt gtcgtcgctg gagggccacg agacgcccta ccccgagcag taccgggtgc
      481 cggcggccac cgcccgcctg gtgaacgcca cccacgccgc cggggcgcgg gtcgtcgcca
      541 tcgggaccac ggtcgtacgg gccctcgaga cggtgaccga ccggtcgggc accacccacc
      601 ccggagaggg ctggaccgag gaggtcatca cccccgagcg gggcgtgcgg gccgtcgacg
      661 gcttgctgac cggctggcac gagcccgagg cctcgcacct cctgatgctg gaggccgtcg
      721 ccgggcgccg tcccctggag ctggcgtacc aggcggccta cgacaacggg tacctctggc
      781 acgagttcgg tgacagccac ctgctccttc cgtcatgatc cagcccatgc ccgcccgcac
      841 cgcaccgtcg ctgcccgagg gtcggcgcaa cgtcctctac gccgtccggc gccggggcga
      901 ggcctcggcc gagcaggtgg ccgagtccct ggccatgacg gtcagcggcg cccgccagca
      961 cctgagcgcc ctggtcgacg ccggcctggc cgaggccacc gagacggagg ccaacgggcg
     1021 accgggccgg cgcaccctcg tctacagcgt gaccccgacc ggtgacgccc tcttccccaa
     1081 ggcgtacggc gagctgacca acgagctgct gggcttcctc gacgacgagg agcccgagac
     1141 cgtcaaccgc ctgttcgccc gccgccggga cgcccgcacg gccaacgccc agcgtcgcct
     1201 cgcccctctc aggaccttgg ccgaccgggt ggccgagctg acccgcatcc tggacgagga
     1261 cgggtacctg gcctcgtggg aggccaacgc cgacggcagc ttcctgatcg tcgagcacaa
     1321 ctgcgccatc tgggccgtgg ccagtcgcta cggccaggcc tgcagcagcg agatcgagtt
     1381 catccagacc gtgctcgacg acgcccgagt cgagcgcatc cagcacatgg tcgagggcgc
     1441 ccgccgctgc gcctacctcg tcacccctct gggctgatcg cggcgtcagg cggcgccggc
     1501 ctcgcagacg ttgaccgggt gggtcgccca ctccgaggaa gggagccgcc agtagctgcc
     1561 gtccgcgcag tcgagggcgg gattgacctc ggcgaacacg ccggcgtcga cgttgtcgta
     1621 gccgggcacc gaccagacat gcaccatgta ctgggtcgag tcgagcatga acccgccgac
     1681 ctcgctgcac tgggcgtcgg tgaccgggag gtcggcgccg aacggggact cgatctcgcc
     1741 gttgacgtac ttgatgcaga tctgctcgtg gtagtgccag acgtcgtttt cgctggcgaa
     1801 gcccacgggc gcggtgctgg tggtggcgta gtacatgaag ccggccaccg gggagtcggg
     1861 ctcgctgccg tcgtagatga tcgagagggg gtgacggagg tcctcgtcgt cgaccacccc
     1921 gtcgaagttc agggccgggc cgctctgggt cacgtagtgg gtgcccagcc ccggcgagta
     1981 ggggccggcc cggcgatagc cggcggcttc ggcgtcggcc acggtcgggt attgggccgc
     2041 gacctcgcgg gtgatggcca actggcgctt gagctccaac tgcgtggcgg gtgacatggg
     2101 ctgccgaacg atcgcgttgt ggtggccgtt ggtcagcaag gaaaggcccc gatcgctgac
     2161 ggcggcggcg gccgcggcgg gcgccgccgc tccgccgtgg gcgtggccgt cggccgagac
     2221 ggcggatccc cctccggcga cggccgtagc tcccacgccg tcggtgccgt gggcatggtt
     2281 ccgggcctcg ggggaggcca ggacggcgct ggcgaggacg acgatggcca ggggcacggc
     2341 gacgctcagg ccggggggca gcgtgacctc ggccaggcgg gggcggaaga gcagggcgat
     2401 ggccatcagg atcagacctg cttcgagggc cacgcaggtg aggtcgacga ccgagacctc
     2461 ctcggcgatg cccgagtgcg gcccgacggg caggcccacc gtacggctca cggcccagac
     2521 ggccatgaac cccaggttgc tcaggatggt gaacccgagg atggcccggg acggctgggt
     2581 ccacaggtag aacgccacca gcagctggag ccagccggcc acggcgaagg ccacgccctc
     2641 cacggagaac tcgtccatgt gggacgggac catcacgaag tggatgacgg cggcggcggc
     2701 ggccagcgac gccatggcca tgagcacggg cgggggcgag gcgacacgct caaaggtgtg
     2761 cgactgcggt gcgaccgtgg tggccatgat ccattccccc cgatgcgtcg gctgaccacc
     2821 ccccggcggt cgacggacaa gcgtagaccc gcgggtagaa ctcggttcgg aaacccgctc
     2881 agacgctgac ggcgaccccc gccacgaacg acgccgtgac cccgacggcg gcgaggtcgg
     2941 ccagagcttc gacccgcggc cggtcgagga cgcacaggtc ggcggcggcg ccggcctcga
     3001 cccgccgggg tgggccgccg ggatcgtcgg cggcccccag gaagaggccc agggcccggc
     3061 ccggggccag gcgctcgtcg tcgcccatga ttgcccccga gggggtgcgc cggttgacgg
     3121 ccgcggccat ggcccgccac gggtcggccg ggccgtaggg ggcgtcggtc ccggctccga
     3181 cgcggacgcc gccgtcgagc agggtggcgc agcgccacag gtcgggctgt tcgccctcgt
     3241 cgacctccgc gcggtaccgg tccccccgct cggcgacgaa accgggttgg gtgatgacgg
     3301 tgaccccgag ggcggccagc gccggcaggg cggtggccgg gatgacggcg ccgtgctcca
     3361 cccgatccgt gggccaggcc cccacctcgg cccaggcggc caggaggagg gccaacgcgt
     3421 cggccgtcac gcagtggacg gcgaccggtc ggccggc
//
LOCUS       DASTFV010000634         6028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2409821, whole genome shotgun sequence.
ACCESSION   DASTFV010000634 DASTFV010000000
VERSION     DASTFV010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2409821"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1586
                     /locus_tag="VFP02_11630"
     CDS             <1..1586
                     /locus_tag="VFP02_11630"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9543730.1"
                     /translation="ILFRYLNTTYFGDGAYGIGAAAQSYFGKPVADLTLSEAALLAGA
                     IASPGRFGPRVNAETAERRRLAVLRAMRDQDLIGRHQFLGARRQHIWYAPLGPPPGPA
                     TIVQPPPRGGASAYPYFVDYVRRVLEERYGHRQLYRGGLRIETTLDPGLQAQAEASVA
                     ETLSGTSAPLEMSLVSVEPATGHVRALVGGRDFDASQVNLALGGSAGMQPGSSFKTFV
                     LATALEHGIDPETMFDAPASMDFPGCDPSCTINNYDFEDHGSLTLRAATHQSINTVYA
                     QLIDRLGPQAVAEMAQRLGVSAIDPARPYGISLALGAYEVSPLDMAAGYATLANRGRR
                     QHVTPILRVLDRRGNVLEDHRAPEGEQVIAPEVADTVSNVLEGVIAGGTGEAADIGRP
                     AAGKTGTTQEYRAAWFAGYTPQLSTAVWMGYSDIPRPLVGIKGAGSVSGGSFPAETWA
                     RYMRAAMAALPVVDFVEPGPLPDGAPYGASPPPVVEAPAAPAPPPAPVPFGASAGEPL
                     PAAVIPDDCGGPCTRDLVP"
     gene            1619..2836
                     /locus_tag="VFP02_11635"
     CDS             1619..2836
                     /locus_tag="VFP02_11635"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET9543731.1"
                     /translation="MGRTTDDPPTLEDALRVPDLEDEELIKTLWDGLADAPVVEPPVA
                     PWESFVGPAAEPFAVPAADPATGDEPDGVDREPESYVPEVEVPLTRRQAKRRRRELKD
                     LKKVEKLRLRRHRILPRTVIGIATMLLFFGLGIGVAGAILYAYYDWRLSENEQRVGEL
                     SASLEDRLLQANEELDLSGANAIQAIRREARPLRALLAEERVVGNLTGRLDGSVWFVE
                     TLDENGAPAVGSAFVAASGDTESLLVTSFGAVAAATTEPAPTIGVRSGDETLEATLYN
                     WDPEHDLALLVVPRGGLEPLPWADNDSLAHIVGARVWAAEGIGGNGVSVSPGSVIDSS
                     DVGLQHTAALNQAFRGGPLVNGRGEVVGIASIGYAPLNYAGGDVSFGVPVQAACERIL
                     NCTGDVPGAGSDR"
     gene            complement(2864..4414)
                     /locus_tag="VFP02_11640"
     CDS             complement(2864..4414)
                     /locus_tag="VFP02_11640"
                     /inference="COORDINATES: protein motif:HMM:NF017669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HET9543732.1"
                     /translation="MPDAPVPSDFAARITQGYAAEGAVVGLGRGVLDGTTHPDAVVQI
                     PAAMCNRHGLIAGATGTGKTVTLQVMAEQLSAIGVPVFAADMKGDLSGIAHPAEPNDR
                     LTGRATELGIEWTPNGSPTAFLSLGGVGPGVPVRAPVSAFGPELLAKVLGANETQRSS
                     LALVFRYADEHGLPLLDLADLRTVLHFLTGDEGKADLKAIGGVSSATAGVLLRSISEL
                     EGQGGDAFFGEPEFAVTDLLRLQGERGVISCLELPAVQDRPRLFSTFLMWLLASLFET
                     LPEVGDLDQPKLVFFFDEAHLLFADASDEFLDQVAQTVRLIRSKGIGVFFVTQLPDDV
                     PDVVLAQLGSRIQHALRAHTPRDAKALKAAVSTYPTTEDYDLEKALTELGTGEAIVTI
                     LSEKGAPTPVVWTKMLPPSSFLGALDPATVKAAGEASPLWPTYAVEIDRESATELLAA
                     KMGVPSPTATAPAKTKPKKAGKAVPKKTKGHDAPDTSAVTDYLKSREGRSMLNTIARG
                     VFSMLKKS"
     gene            4530..>6028
                     /locus_tag="VFP02_11645"
     CDS             4530..>6028
                     /locus_tag="VFP02_11645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9543733.1"
                     /translation="MTRVAGVASPRQGARPFTVAVAVTVVLMALSVVAALGWRSQLQG
                     DGREQFDLDATAAQQVVAQRLQSTVNVLQATRSTVDGDPSSVTADAFDQTVAAALSAE
                     LPSLASVALVERVAEEDIEQLQADRVAAGLSDFEVLSVEEGGAPPAIATFEASTRAAP
                     ILSGYDLRAVPALAEALTPGSNANHHISPRLEALPADVLELHPDLGESAFAMVIPAGP
                     GSWVLALLSGDELAEQAAAVDDELVVALAVGDSPVGASTVRDGRAVDLDAVPSDARTV
                     RPFDIDGGPLLVTVADIDGLAGGGWREPGLLLGAGLALSILVGALVLVLAQGRAGALR
                     VASEAQLARDRSEENFRAVVQHLSDLVLVTDASTMISFVTPSVARLLGREASSLPGGF
                     LGDLVHPDDRALLAALTSRSGVSEKGLIRFRHSDGSDRSFEVVVANRLEDPAIGGLVL
                     TAHDVTDRVKLEDRLAHEATHDDLTGLPNRALIHDRLEHALLRADRTGGR"
BASE COUNT          776 a         2237 c         2139 g          876 t
ORIGIN      
        1 agatcctctt ccgctacctg aacacgacct acttcggtga cggggcctac ggcatcggcg
       61 ccgccgccca gtcgtacttc ggcaagccgg tcgccgacct caccctgtcc gaggctgcgc
      121 tcctggcggg cgccatcgcc tcacccgggc gcttcggccc ccgggtcaac gccgagacgg
      181 ccgagcgccg gcggctcgcc gtcctccggg cgatgcggga ccaggatctg atcggccggc
      241 accagttcct gggcgcccgc cgtcagcaca tctggtacgc cccgctcggc cccccgccgg
      301 gaccggcgac gatcgtccag cccccgcccc ggggcggggc gtcggcctac ccctacttcg
      361 tggactacgt ccgtcgggtg ctcgaggagc gctacggcca ccgtcagctc taccggggtg
      421 gcctgcgcat cgagaccacc ctcgatcccg gcctgcaggc ccaggccgag gcctcggtgg
      481 ccgagacgct gagcgggacc agcgccccgc tggagatgtc cctggtgtcg gtcgagccgg
      541 ccacgggcca tgtccgcgcc ctcgtcggtg gccgggactt cgacgccagc caggtcaacc
      601 tcgccctcgg cggctccgcg ggcatgcagc ccggctcgtc gttcaagacc ttcgtgctgg
      661 ccacggccct ggagcacggg atcgacccgg agacgatgtt cgacgctccg gcgtccatgg
      721 acttccccgg ctgtgacccg agttgcacga tcaacaacta cgacttcgag gaccacgggt
      781 ccctgaccct ccgcgccgcc acccatcagt cgatcaacac cgtctacgcc cagctcatcg
      841 accggctcgg tccgcaggcc gtggccgaga tggcccagcg cctcggtgtg tccgccatcg
      901 acccggcccg gccctacggc atctcgctgg cgctgggggc ctacgaagtg tcgcccctgg
      961 acatggccgc gggctacgcc accctggcca accggggccg gcgccagcac gtcacgccga
     1021 tcctgcgggt cctggaccgc cggggcaacg tgctcgagga tcaccgggcg cccgaggggg
     1081 agcaggtcat cgctcccgag gtggccgaca ccgtgtccaa tgtcctcgaa ggcgtgatcg
     1141 cgggcggtac cggcgaggcc gccgacatcg gccggcccgc ggcgggcaag accggcacca
     1201 cccaggagta ccgggccgcc tggttcgccg gctacacgcc ccagctctcg accgcggtgt
     1261 ggatggggta cagcgacatc ccccgaccgt tggtcgggat caagggtgcg ggcagcgtga
     1321 gcggggggtc gttccccgct gagacctggg cccggtacat gcgggcggcg atggccgccc
     1381 tgccggtcgt ggacttcgtc gagcccggcc cgctgcccga cggcgcgccc tacggggcca
     1441 gcccgccgcc cgtggtcgag gcccccgcgg ccccggcccc cccgcccgcc ccggtgccct
     1501 tcggggccag cgcgggtgag ccgttgccgg ctgccgtcat ccccgatgac tgcggcggcc
     1561 cctgcacacg agatctggtc ccctgaccgg gaaggacatg gcgatgcgac gagaagactt
     1621 gggacgcacg acggacgatc cgccgacgtt ggaggacgcc ctgcgcgtgc ccgatctcga
     1681 ggacgaagag ctgatcaaga ccctgtggga cggcctggcc gacgcgccgg tcgtcgagcc
     1741 gcccgtggcg ccgtgggagt cgttcgtggg gcccgcggcg gagccgttcg ccgtgccggc
     1801 ggccgatccg gccaccgggg acgagcccga cggggtcgac cgtgagcccg agtcgtacgt
     1861 gcccgaggtc gaggtcccgc tcacccggcg ccaggccaag cggcgccgcc gcgagttgaa
     1921 ggacctgaag aaggtcgaga agctgaggct gcgccggcac cggatcctgc cccgcaccgt
     1981 gatcgggatc gccacgatgc tgctgttctt cggcctcggg atcggcgtcg ccggcgccat
     2041 cctctacgcc tactacgact ggcgcctctc cgagaacgag cagcgggtgg gcgagctgtc
     2101 cgccagcctc gaggatcgcc tgctccaggc caacgaggag ctcgacctgt ccggcgcgaa
     2161 cgccatccaa gccatccggc gtgaggctcg gccgctgcgg gcgttgctcg cagaggagcg
     2221 tgtcgtcggt aacctgaccg gtcgcctgga cggctcggtc tggttcgtcg agaccctcga
     2281 cgagaacggc gctcccgccg tcggctcggc cttcgtggcc gcgtcgggcg acaccgagtc
     2341 gctgctcgtc acctcgttcg gggccgtggc tgcggccacg acggagccgg cgcccaccat
     2401 cggtgtccgg tccggggacg agaccctgga ggccaccctc tacaactggg acccggagca
     2461 cgacctggcc ctgctcgtcg tcccccgcgg gggcctcgag ccgctgccgt gggccgacaa
     2521 cgactcgttg gcccacatcg tgggagcgcg ggtgtgggcg gccgagggca tcgggggcaa
     2581 cggggtcagc gtgagcccag gctcggtcat cgactcgtcc gacgtcggcc tccagcacac
     2641 cgcggccctg aaccaggcgt tccgcggggg cccgctggtc aacggccggg gcgaggtcgt
     2701 gggcatcgcg tccatcggct acgccccgct caactacgcg ggtggcgacg tcagcttcgg
     2761 cgtccccgtc caggccgcct gcgagcgcat cctcaactgc accggcgacg tccccggcgc
     2821 gggctcggac cgctgatccc ccgttcccga acgagcggcg gtgtcaggac ttcttgagca
     2881 tggagaagac gccgcgggcg atggtgttga gcatgctgcg gccttcgcgg ctcttcaggt
     2941 agtcggtcac cgcgctggtg tccggcgcgt cgtggccctt cgtcttcttc ggaacggcct
     3001 tgcccgcttt cttgggcttg gtcttggcgg gggcggtcgc ggtcggcgag ggtacgccca
     3061 tcttggcggc caggagctcg gtggccgact cccggtcgat ctcgacggcg taggtgggcc
     3121 acaggggtga cgcctcgccc gccgccttga ccgtcgcggg gtcgagggca ccgaggaagc
     3181 tcgagggcgg cagcatcttg gtccacacca ccggggtcgg ggcgcccttc tcggacagga
     3241 tcgtcacgat cgcctcgccc gtgcccagct cggtgagggc cttctccagg tcgtagtcct
     3301 cggtcgtcgg gtacgtggac acggccgcct tcagggcctt ggcgtcccgc ggcgtgtggg
     3361 cccgcagggc gtgctggatc cggctaccga gctgggccag caccacgtcg ggcacgtcgt
     3421 cggggagctg ggtgacgaag aagacaccga tccccttgga acggatcagg cggacggtct
     3481 gggccacctg gtcgaggaac tcgtcactgg cgtcggcgaa gaggaggtgg gcctcgtcga
     3541 agaagaagac gagcttgggc tggtcgaggt cgccgacctc gggcagggtc tcgaacagcg
     3601 acgccagcag ccacatgagg aaggtggaga agagcctcgg ccggtcctgg accgcgggga
     3661 gctccaggca gctgatgacg ccccgctcgc cctggaggcg gagcaggtcg gtcaccgcga
     3721 actcgggctc gccgaagaag gcgtcgccgc cctggccctc cagctcgctg atcgagcgca
     3781 ggaggacgcc ggcggtggcc gaggacacgc cgccgatggc cttcaggtcc gccttgccct
     3841 cgtcgccggt gaggaagtgc aggaccgtgc gcaggtcggc caggtcgagc aggggcagac
     3901 cgtgctcgtc ggcgtagcgg aagaccaggg ccagggacga gcgctgggtc tcgttggcgc
     3961 cgagcacctt ggccaggagc tcggggccga aggccgacac gggggcccgg acgggcacgc
     4021 cggggcccac gccccccagc gagaggaagg cggtcggact gccgttcggc gtccactcga
     4081 tgcccagctc ggtggcccgc ccggtcaacc ggtcgttggg ctcggcgggg tgggcgatgc
     4141 cgctgaggtc gcccttcatg tcggccgcga acaccggcac gccgatggcc gagagctgtt
     4201 cggccatgac ctggagggtg accgtcttgc ccgtcccggt ggcgcccgcg atcagcccgt
     4261 ggcggttgca catggccgcc gggatctgga ccacggcgtc ggggtgggtg gtgccgtcga
     4321 ggacgccgcg gcccagtccg accaccgccc cctccgcggc gtagccctgg gtgatccggg
     4381 cggcgaaatc ggacgggacg ggcgcatcgg gcatggtcct gcgatcgtag gggccgctct
     4441 ggttcgtgtc ccggccttgg cctcgatccc ctgcacacgc tccgtgcacg tgcaaccgcg
     4501 tgacgtggtc aacgggctag actgtgtcca tgacccgcgt cgccggggtc gcctcccctc
     4561 gccagggggc tcgcccgttc accgtggccg tcgccgtgac ggtcgtgctc atggctctca
     4621 gcgtggtcgc cgccctcggc tggcgcagcc agctccaggg cgacggccgc gagcagttcg
     4681 acctggacgc caccgcggcc caacaggtcg tcgcccagcg cctacagagc acggtcaacg
     4741 tcctccaggc cacccggtcc accgtcgacg gcgatccgtc gtcggtcacc gcggacgcct
     4801 tcgaccagac cgtggccgcc gcgctgtccg ccgagttgcc gtcgctggcc tccgtggcat
     4861 tggtcgagcg ggtggccgag gaggacatcg agcaactgca ggccgatcgc gtcgccgccg
     4921 gactgagcga cttcgaggtg ctctccgtcg aggagggtgg ggcgccgccc gccatcgcca
     4981 ccttcgaggc cagcacccgg gccgccccga tcctgtccgg ctacgacctc cgcgccgtgc
     5041 ccgccctggc cgaggcgctc actcccggct cgaacgcgaa ccaccacatc agcccccgcc
     5101 tggaggccct gcccgccgac gtcctcgagc tgcaccccga cctcggcgaa tccgccttcg
     5161 ccatggtcat cccggcggga cccggttcct gggtcctggc cctgctgtcc ggcgacgagc
     5221 tggccgagca ggccgccgcg gtcgatgacg agctggtcgt ggccctcgcc gtcggcgact
     5281 cccctgttgg cgcctcgacc gtccgggacg gtcgcgccgt cgacctggat gccgtgccct
     5341 ccgatgcccg gacggtccgc cccttcgaca tcgacggcgg tccgctgttg gtcacggtgg
     5401 ccgacatcga cggcctcgcg ggcggcgggt ggcgcgagcc gggcctgctc ctcggcgctg
     5461 ggctcgccct cagcatcctc gtcggcgccc tggtcctggt cctcgcccag ggccgggccg
     5521 gcgccctccg ggtcgcctcg gaggcccagc tcgcccggga ccgttccgag gagaactttc
     5581 gggccgtggt ccagcacctg tccgatctcg tcctcgtgac cgacgcgtcc accatgatca
     5641 gcttcgtcac cccgtcggtc gcccgcctcc tcggccgtga ggcgtcgtcc ctgcccggcg
     5701 ggttcctcgg cgacctggtc catcccgacg accgtgccct gctggcggcc ttgacgtcac
     5761 ggtcgggcgt ctccgagaag ggcctgatcc gcttccgtca cagcgacggt tcggaccgca
     5821 gcttcgaggt cgtggtcgcc aatcgcctcg aggaccccgc catcggtggc ctggtcctca
     5881 ccgcccacga cgtgaccgat cgggtcaagc tcgaggaccg gctggcccac gaggccaccc
     5941 acgacgacct caccggcctg ccgaaccggg ccctgatcca cgaccgcctc gagcacgcgc
     6001 tcctgcgagc cgatcggacc ggcggccg
//
LOCUS       DASTFV010000635         4136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2412125, whole genome shotgun sequence.
ACCESSION   DASTFV010000635 DASTFV010000000
VERSION     DASTFV010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2412125"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VFP02_11650"
     CDS             complement(<1..1322)
                     /locus_tag="VFP02_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543734.1"
                     /translation="MRRTEGDQVVKGDGSGTRAELRRLLEVTALTGLAIVQPTLDVLG
                     RSPETFVFRRVDGTQLAVFAVLVAVVPALVLWGAGVLTGLLGARVRAGVHLATLAGLL
                     VLAVLVALKSADVLFGVAALAVAVGIAALGVVLYRRVAVARLFLEYLALLPVLALGAF
                     LFTSPASALLSGGEYDIVDDPGTTTPLVVLVLDEFPTAAILDRNGEVDAERFPNLASL
                     AEESRWYRNYTTVSNFTLHAVPALLSGMAPDADDVPLVADHPDNLFTLLGGTYEFNVS
                     EAITRLCPGELCPSELTATDHAGLRGITDDTVDVVQELVSLDAFPGPESDFFVEEVTD
                     PTDPTFEHGRDAEVQPTRFRDFLGALAPTAQPGLHYLHLVLPHEPWRFFPDGTEYASP
                     GRDPRGDPDGRWTGTWPARFNRLRFELQARYVDTLVGQTVARLQESSL"
     gene            complement(1392..>4136)
                     /gene="rpoC"
                     /locus_tag="VFP02_11655"
     CDS             complement(1392..>4136)
                     /gene="rpoC"
                     /locus_tag="VFP02_11655"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET9543735.1"
                     /translation="SDMLKGKQGRFRQNLLGKRVDYSGRSVIVGGPQLRLHQCGLPKL
                     MALELFKPFVMKKLVDEELAQNIKSAKRMVERRKPQVWDVLDDVIKEHPVLLNRAPTL
                     HRLGIQAFEPVLVEGKAIQIHPLVCQAFNADFDGDQMAVHLPLSAEAQAESRILMLSA
                     HNVLSPAHGRPLVTPTQDMVIGAYYLTQGPGEVNPDDPHQVRRHKDDEWRPIKTFRRA
                     ESVERAYEGGDISLHEPVILRVPELEETPANGKAANYVPTTTGRVLFNEVLPEGFEYV
                     NHVVRKGDMGAIVDVLADAYPKAVVAQTLDALKNRCFRFATKSGLTVSVDDVRTPADK
                     QKILDDHEKQAEKVEDQFRKGVITDGERRQKEVEIWNDATAMIQSQLETDLASNPFNP
                     IDMMVRSGARANPMQIRQIAGMRGLVANPRGDMIPRPIKSNYREGLHVLEYFIATPGA
                     RKGLVDTALRTADSGYLTRRLVDVAQELIIQEQDGEETGPVRGIWLEDVEEDTPGKRT
                     YLETRLYSRTLADDVKLSDGSTMSAGTIVWDDELAALRDDPDVTRLRVRSPLTDEAEV
                     GVSLAAYGGSLATGKDIEVGEAVGVIAAQSIGEPGTQLTMRTFHTGGIAGSDIAGGLP
                     RVVELFEARSPKGKATLARTSGVVRVDEEEGRGRTVTIVADDGEEDEYSIPLAVRLEV
                     TDGDEVAAGDALVEGPRDPKELLEIKGVRETQKYLVDEVQKVYRDQGVSIHDKHIELI
                     VRQMTRRVAVSEPGDSGFLPGERVDQWVFTRINRELVQDGKVPAEGRPELMGITKASL
                     ATDSWLSAASFQETTRVLTEAAIESRSDTLVGLKENIIIGKLIPAGTGMAQYRDIGTT
                     APDYVPMEYYSSADEEQDLAEWLAGRSETGDSGVGILAGAPGGAEVIDLPTGEDEASA
                     "
BASE COUNT          575 a         1405 c         1516 g          640 t
ORIGIN      
        1 cacagggagc tctcctggag gcgagcgacg gtctggccca cgagggtgtc gacgtagcgg
       61 gcctgcagct cgaagcggag acggttgaag cgggccggcc aggtccccgt ccagcgtccg
      121 tcgggatcac cgcgcggatc gcgccccggc gaggcgtact cggtgccgtc cgggaagaag
      181 cgccagggct cgtgaggcag gacgaggtgc aggtagtgca gtcccggttg cgccgtcggc
      241 gccagggccc caaggaagtc ccggaaccgg gtgggttgga cctccgcgtc ccggccgtgc
      301 tcgaacgtcg gatccgtggg atcggtgacc tcctcgacga agaagtccga ctccgggccg
      361 gggaacgcat cgagggacac cagttcctgg acgacgtcca ccgtgtcgtc ggtgatgccc
      421 cgcagcccgg cgtgatccgt cgccgtcagc tccgacgggc agagctcgcc cgggcagaga
      481 cgggtgatcg cctcggacac gttgaactcg taggtgccgc cgagcagggt gaagaggttg
      541 tccgggtggt cggccacgag gggcacgtcg tcggcgtccg gggccatccc cgacaggagg
      601 gcgggcacgg cgtgcagcgt gaagttgctg accgtcgtgt agttccggta ccagcgcgac
      661 tcctcggcca gcgaggcgag gttcgggaag cgttcggcgt cgacctcacc gttgcggtcg
      721 aggatcgccg ccgtcgggaa ctcgtcgagg accaggacga ccagcggcgt cgtcgtcccg
      781 ggatcgtcga cgatgtcgta ctcgcccccg ctgagcaggg cggacgccgg cgacgtgaag
      841 aggaaggcgc cgagggccag gaccgggagc agcgcgaggt actcgaggaa gagccgggcc
      901 accgcgaccc ggcggtagag caccaccccg agggcggcga tcccgacagc caccgccagc
      961 gcggcgacgc cgaagagcac gtccgccgac ttgagcgcca cgagcacggc gaggacgagc
     1021 aggccggcca gcgtggccag gtggacacca gcccgcaccc gggcaccgag gagcccggtc
     1081 aggacgcccg cgccccacag gaccagggcc gggacgaccg ccaccagcac cgcgaacacg
     1141 gccagctgcg tcccgtccac gcgccggaag acgaaggtct ccgggctgcg cccgaggacg
     1201 tcgagggtgg gctggacgat ggccagcccc gtgagcgccg tcacctcgag caggcgccgc
     1261 agctcggccc gtgtcccgct cccgtcgccc ttcaccacct ggtcgccttc cgttcgccgc
     1321 accgtagacg cgcttctggc gcccgattcg gtcgcggtga cgaccgaacc aggcgccaga
     1381 agggatgcgg tctaggcgga ggcttcgtcc tcgccggtgg gcaggtcgat gacctcggcg
     1441 ccgcccggag cgccggcgag gatgcccacg ccggagtcgc cggtctcgct gcgcccggcc
     1501 agccactcgg ccaggtcctg ctcctcgtcc gccgaggagt agtactccat ggggacgtag
     1561 tccggggcgg tggtcccgat gtcgcggtac tgggccatgc ccgtgccggc cgggatcagc
     1621 ttgccgatga tgatgttctc cttcagcccg acgagggtgt cggagcggga ctcgatggcc
     1681 gcctcggtca gcacccgggt ggtctcctgg aaggaggccg ccgagagcca cgagtcggtg
     1741 gccagcgagg ccttggtgat ccccatcagc tcgggccggc cctcggccgg gaccttgccg
     1801 tcctgcacca gctcgcggtt gatccgggtg aacacccact gatcgacccg ctcgccgggg
     1861 aggaagccgc tgtcgccggg ctcggagacc gccacccggc gggtcatctg ccggacgatg
     1921 agctcgatgt gcttgtcgtg gatcgacacg ccctggtccc ggtagacctt ctggacctcg
     1981 tcgacgaggt acttctgggt ctcccggacg cccttgatct cgagcagctc cttggggtcg
     2041 cgggggccct cgaccagcgc atcacccgcg gcgacctcgt cgccgtcggt gacctcgagg
     2101 cggacggcca gcgggatgct gtactcgtcc tcctcgccgt cgtcggccac gatcgtgacc
     2161 gtgcggcccc gaccctcctc ctcgtcgacc cggaccacac ccgaggtgcg ggccagggtg
     2221 gccttgccct tggggctgcg ggcctcgaag agctccacga cccggggcag gccgccggcg
     2281 atgtccgagc cggcgatgcc gccggtgtgg aacgtccgca tggtcagctg cgtgccgggc
     2341 tcgccgatgg actgggcggc gatgaccccg acggcctcgc ccacctcgat gtccttgccg
     2401 gtggccagcg agccgccgta ggcggccagg gagaccccga cttcggcctc gtcggtcagc
     2461 ggggagcgga cccggagccg ggtgacgtcg gggtcgtcgc gcagggcggc gagctcgtcg
     2521 tcccagacga tggtgccggc ggacatggtg ctgccgtcgg agagcttgac gtcgtccgcc
     2581 agcgtccggc tgtagaggcg ggtctcgagg taggtccgct tgcccggcgt gtcctcctcg
     2641 acgtcctcca gccagatgcc acgcaccggg ccggtctcct cgccgtcctg ctcctggatg
     2701 atcagctcct gggcgacgtc gaccagacgc cgggtgaggt acccggagtc ggcggtccgc
     2761 agggcggtgt cgaccaggcc cttacgggcg cccggcgtgg cgatgaagta ttcgaggacg
     2821 tgcaggccct cgcggtagtt cgacttgatc ggccggggga tcatgtcgcc tcgggggttg
     2881 gccaccaggc cccgcatgcc cgcgatctgg cggatctgca tggggttcgc ccgggcgccg
     2941 gagcgcacca tcatgtcgat ggggttgaac gggttcgagg ccaggtcggt ctccagctgg
     3001 ctctggatca tggccgtggc gtcgttccag atctccactt ccttctggcg gcgctcaccg
     3061 tcggtgatga cccccttgcg gaactggtcc tcgaccttct cggcctgctt ctcgtggtcg
     3121 tccaggatct tctgcttgtc cgccggcgtc cggacgtcgt ccaccgagac ggtcagcccg
     3181 gacttggtgg cgaagcggaa gcaccggttc ttgagcgcgt cgagcgtctg ggccacgacg
     3241 gccttcgggt aggcgtcggc caggacgtcg acgatggcgc ccatgtcgcc cttgcggacg
     3301 acgtggttca cgtactcgaa gccctcgggg agcacctcgt tgaagaggac ccgaccggtg
     3361 gtcgtcggca cgtagttggc cgccttgccg ttggcggggg tctcctccag ctcgggcacc
     3421 cgcaggatca cgggctcgtg gagggagatg tcgcccccct cgtaggcccg ctccaccgac
     3481 tcggcgcggc ggaacgtctt gatcgggcgc cactcgtcgt ccttgtggcg acggacctgg
     3541 tgcggatcgt ccgggttgac ctcacccggg ccctgggtca ggtagtaggc cccgatgacc
     3601 atgtcctggg tgggcgtgac cagcgggcgg ccgtgggccg ggctgagcac gttgtgggcc
     3661 gacagcatga ggatccggct ctcggcctgg gcctccgccg acaggggcag gtgcacggcc
     3721 atctggtcgc cgtcgaagtc ggcgttgaac gcctggcaga ccagcgggtg gatctggatg
     3781 gccttgccct cgaccagcac cggctcgaag gcctggatgc cgaggcggtg cagggtgggc
     3841 gcccggttca gcaggaccgg gtgctccttg atgacgtcgt cgaggacgtc ccacacctgg
     3901 ggcttgcggc gctcgaccat gcgcttggcc gacttgatgt tctgggccag ctcctcgtcg
     3961 accagcttct tcatgacgaa gggcttgaac agctccaggg ccatgagctt gggcaggccg
     4021 cactggtgca ggcgcagctg agggccgccg acgatgaccg aacggccgga gtagtcgacg
     4081 cgcttgccga gcaggttctg gcggaaccgg ccctgcttgc ccttcagcat gtcgga
//
LOCUS       DASTFV010000636         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2413121, whole genome shotgun sequence.
ACCESSION   DASTFV010000636 DASTFV010000000
VERSION     DASTFV010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2413121"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VFP02_11660"
     CDS             complement(<1..359)
                     /locus_tag="VFP02_11660"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET9543736.1"
                     /translation="MLLLVILALAALSGWRRGFAMVLLGYLGLLVGLALGAWAATQAG
                     LLVSADSSVRRLLTAVVVFFLVAAACHAVGNLLGIRVRSLLGGRWTSRADAIGGAVVA
                     TIVAAIAMWFVALTLSNV"
     gene            492..698
                     /locus_tag="VFP02_11665"
     CDS             492..698
                     /locus_tag="VFP02_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543737.1"
                     /translation="MDIDVDTELAPLSLAAARALFRAPQAEDACWADVHMPDGTVRAC
                     GRPEATNLGLCAEHRMELFGLAAC"
     gene            complement(708..2084)
                     /locus_tag="VFP02_11670"
     CDS             complement(708..2084)
                     /locus_tag="VFP02_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9543738.1"
                     /translation="MAGTRMVVVGGDAAGMSAATQARKRRPSQELEIVAFERGRHASY
                     SACGLPYYAADVVKDWRDLVARTPEEFASQDIEVRTRHEVEAVDLEAGRVRVRDLDAG
                     RTHEEAWDVLVVATGSEPLRPPIPGIDLPGVYGLSVLQDGIELRRAVDAGPDRAVIVG
                     GGYIGLEAAEALVHRGIPTALVEAAPQVMNRLDPDMGELVSQAVRDIGVELHLEEKVE
                     AIEGGDRVRAVRTAARTLEADLVVLGLGTRPAVGLAEASGIRLGPSGAIAVNPRMHTP
                     HEGVWAAGDCAESRHLVSGKPVSYHLGTIANKQGRVCGINVGGGYATFPGVLGTAVSK
                     VCEVEVARTGVSEAELGELGLEWERASVDSTTTAGYLPGAAPIRIKVLAERRSGRLLG
                     AQIVGKEGAAKRIDVFATAIWNGMAVDEMVNLDLSYAPPFSPVWDPVLIAVRKGSEQV
                     DRTLCESL"
BASE COUNT          305 a          857 c          822 g          285 t
ORIGIN      
        1 acgttgctca gggtcagggc gacgaaccac atggcgatgg cggcgacgat ggtggccacc
       61 accgccccgc cgatggcgtc ggcccggctc gtccagcgcc cgcccagcag cgatcggacc
      121 cggatcccga gcaggttgcc gacggcgtgg caggcggccg cgaccaggaa gaacaccacc
      181 acggcggtca gcagccgccg caccgagctg tcggccgaga ccagcagccc ggcctgggtg
      241 gccgcccacg cccccagggc cagcccgacc agcagcccca ggtagccgag cagcaccatc
      301 gcgaagccgc gccgccagcc gctcagggcg gccagggcca ggatgacgag caggagcacg
      361 tcgatgggtt gaaggtcgag tggcatcgct gttccggccg cgggtggggt gaccgtctca
      421 tcctaatcgg tcggaggacg gagcacgggt tgctacaccg cccggacggg cgtgccgatg
      481 ctcactaccg tatggacatc gacgtcgaca ccgagctggc accgctctcg ctggccgccg
      541 cccgggcgct gttccgggcc ccgcaggccg aggacgcgtg ctgggcggac gtgcacatgc
      601 ccgacgggac ggtcagggcc tgcgggcggc ccgaggcgac caacctgggg ctctgcgccg
      661 agcaccggat ggagctgttc gggctggcgg cctgctgagg ccggggctca caggctctcg
      721 caaagggtcc ggtcgacctg ctcggagccc ttgcgcacgg cgatcagcac cgggtcccag
      781 accggcgaga acggcggcgc gtacgacagg tcgaggttga ccatctcgtc gacggccatg
      841 ccgttccaga tggcggtggc gaacacgtcg atccgcttgg ccgcgccctc cttgccgacg
      901 atctgggcgc ccagcaggcg gcccgagcgg cgctcggcca gcaccttgat ccggatcggg
      961 gccgcccccg ggaggtagcc ggccgtggtg gtcgagtcga ccgacgcccg ctcccattcc
     1021 aggccgagct cgcccagctc ggcctcggac acgccggtgc gggcgacctc gacctcgcac
     1081 accttggaca ccgccgtgcc gagcaccccc ggaaaggtgg cgtagccgcc gccgacgttg
     1141 atgccgcaga cacgtccctg cttgttggcg atcgtcccca ggtggtagct gaccggcttg
     1201 ccggacacga ggtggcggga ctcggcgcag tcgccggccg cccacacccc ctcgtggggg
     1261 gtgtgcatgc gggggttgac ggcgatggcg ccgctcgggc cgagccggat gcccgacgcc
     1321 tcggccaggc cgacggcggg ccgggtgccg aggccgagca cgaccaggtc ggcctccagg
     1381 gtccgggcgg cggtccgcac cgcccggacg cggtcgccgc cctcgatcgc ctccaccttc
     1441 tcctccaggt gcagctcgac cccgatgtcc ctcaccgcct ggctgaccag ctcgcccatg
     1501 tcggggtcga gccggttcat cacctggggg gccgcctcga ccagggcggt ggggatgccc
     1561 cggtggacca gcgcctcggc cgcctcgagg ccgatgtagc cgcccccgac gatcaccgcc
     1621 cggtccgggc cggcgtcgac cgcccggcgc agctcgatgc cgtcctggag caccgacagc
     1681 ccgtacaccc ctggcaggtc gatgccgggg atcggcgggc gcagcggctc ggagccggtc
     1741 gccaccacca gcacgtccca ggcctcctcg tgggtgcgcc cggcgtccag gtcgcggacc
     1801 cggacccgcc ccgcctccag gtcgacggcc tcgacctcgt gccgggtccg gacctcgatg
     1861 tcctgggagg cgaactcctc gggggtgcgg gcgaccaggt cgcgccagtc cttgaccacg
     1921 tcggcggcgt agtaggggag cccgcaggcc gagtacgagg cgtgccggcc ccgctcgaag
     1981 gcgacgatct ccagctcctg ggacggccgg cgcttgcgcg cctgggtggc ggcgctcatc
     2041 ccggccgcgt cgcccccgac caccaccatg cgcgtccctg ccacggcccc tcctcgtcgt
     2101 cggctgccga ggctagtctg ccctctccga gcgagggcta ccccgggagg tcgagcaggt
     2161 gaccaggatg ctgttccact gcacccacgg gcgggacgac ccggagcggg cgatcgtgcc
     2221 gttcatcgcc gccaacgtgg ccgccgcctc cggccaggag gcggtggtg
//
LOCUS       DASTFV010000637         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2420251, whole genome shotgun sequence.
ACCESSION   DASTFV010000637 DASTFV010000000
VERSION     DASTFV010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2420251"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFP02_11675"
     CDS             <1..228
                     /locus_tag="VFP02_11675"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9543739.1"
                     /translation="PLRVNAVAPGGVETPIQNDFMTIPEGGDGKKLFKMMTKKGMCSP
                     QDVAATFAHVASDESAYMNGSIVVIDGGITA"
     gene            248..1006
                     /locus_tag="VFP02_11680"
     CDS             248..1006
                     /locus_tag="VFP02_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9543740.1"
                     /translation="MGTYCVTGSARGIGGAIRARIEAAGHSVIGVDRAGQEVEADLAT
                     PEGRLRAIAAVQAAAGDALDGLVVCAGLPPTFKPTETIAQVNYFGAVELLDALRPLLE
                     AAQSGAAVAISSNSLGTVPEADEPLMGPLLAGEVDTATTAAAGLHGIQVYGMSKRAVA
                     RAVRHRAPTWAEAGVRLNAVAPGATQTVLFQETLDDAEIGPFVADFPIPVGRRGTVGD
                     IAEAVWFVLEATFMVGSVVFVDGGSDAQMRPDVV"
     gene            1009..1911
                     /locus_tag="VFP02_11685"
     CDS             1009..1911
                     /locus_tag="VFP02_11685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET9543741.1"
                     /translation="MPGLSLGIAGLHRTFGPDLDHYLDVAQVADEVGIDQLVLADHVV
                     MGQNTGAYPYGEFAFAPEEPWAEPLTLLTAIAAVTTRVRLGTGILITPLRTPASLAKT
                     VATLDTFSGGRVDLGVGVGWQREEYDVAGVPWAERWGRLDDGLRACRALWADGPASLT
                     SPSITFTDIWCRPGPAQARLPVWLGAKATDRNCARVADWGDGWMPLEAKTGSDELFAG
                     IARLRSAFAAADRSEDELLVRAGLPFVRGDDGFDFPASRDAALRLAEAGVTHFSVNLR
                     RSIASAADARRFLEQLAEGLLPLA"
BASE COUNT          290 a          803 c          773 g          346 t
ORIGIN      
        1 ccgttgcggg tcaatgccgt ggcccccggc ggtgtggaga ccccgatcca gaacgacttc
       61 atgacgatcc cggagggtgg ggacggcaag aagctgttca agatgatgac caagaaggga
      121 atgtgttccc cgcaggacgt ggccgccacg ttcgcccacg tcgcgtcgga cgagtcggcc
      181 tacatgaacg gctccatcgt ggtcatcgac gggggcatca cggcctgatc ggccagaagg
      241 ggagtcgatg gggacctact gcgtcacggg gtcagcccgc ggcatcgggg gcgcgatccg
      301 ggcccgcatc gaggcggccg gccactcggt catcggggtg gaccgggcgg gtcaggaggt
      361 cgaagccgac ctggcgacac ccgaggggcg cctgagggcc atcgccgccg tgcaggccgc
      421 cgccggcgac gccctcgacg gccttgtcgt gtgcgccggg ttgccgccca ccttcaagcc
      481 caccgagacc atcgcccagg tcaactactt cggcgccgtg gagctgctcg atgcgttgcg
      541 gcccctcctc gaggcggccc agtccggggc cgcggtggcc atctcgtcca actccctggg
      601 aacggtgccc gaggccgacg agccgctgat gggtcccctc ctggccggtg aggtcgacac
      661 ggccaccacc gccgccgccg gcctgcacgg catccaggtc tacggcatgt cgaagcgggc
      721 cgtggcccga gcggtgcgcc accgggcgcc gacctgggcc gaggccggcg tccggctcaa
      781 cgccgtcgct cccggggcga cccagaccgt cttgttccag gagaccctcg acgacgccga
      841 gatcgggccg ttcgtggccg acttcccgat ccccgtcggc cgacggggta cggtcggcga
      901 catcgccgag gccgtctggt tcgtgctcga agccaccttc atggtcggct ccgtggtctt
      961 cgtggacggc ggcagcgacg cccagatgcg cccggacgtg gtctgagcgt gcccggcctc
     1021 tcgctcggca tcgccggcct ccaccgcacg ttcggcccgg acctggacca ctacctcgac
     1081 gtggcccagg tggccgacga ggtcggcatc gaccagctcg tcctcgccga ccacgtggtc
     1141 atgggccaga acaccggtgc ctatccctac ggcgagttcg ccttcgcccc ggaggaaccg
     1201 tgggctgagc cgctgaccct gctcaccgcc atcgccgcgg tcaccacccg cgtccgactg
     1261 ggcacgggca tcctcatcac acccctgcgg acgccggcat cgttggcgaa gaccgtggcc
     1321 accctcgaca ccttctccgg cggccgagtg gacctcggcg tcggtgtggg ttggcagcgg
     1381 gaggagtacg acgtcgccgg cgtgccctgg gcggaacgct ggggtcgcct cgacgacggc
     1441 ctgcgggcct gccgggcgct ctgggcggac gggcccgcct cgttgaccag ccccagcatc
     1501 accttcaccg acatctggtg ccgacccggt cccgctcagg cccggctgcc ggtctggttg
     1561 ggggccaagg ccactgaccg caactgcgcc cgcgtggccg actggggcga cggctggatg
     1621 ccgctggagg cgaagaccgg gtcggacgag ttgttcgccg ggatcgcccg cctgcggtcg
     1681 gcgttcgccg cggccgaccg ctctgaggac gaactgctcg tccgggccgg cctgccgttc
     1741 gtgcggggcg atgacggctt cgatttcccc gccagtcgcg acgccgccct gcgcctggcc
     1801 gaggcgggcg tcacccactt ctcggtcaac ctccgccgca gcatcgcgtc ggcggccgac
     1861 gcccgccgct tcctggagca actggcagag ggcctccttc ccctggcctg acggggtcgc
     1921 ggccgtcctc gaggtcgatt gaccaagtgc ttgaccttcg tccgtcaggg tcgatatgag
     1981 ggtcagtgcg agcggttcgt ggtcttcact tcggcgcggc gctggtgatc acaatcgtcg
     2041 ccggtgcacc gcacgcctct cctccgaagg cggcaaccgg gagtggtggc cgtgaggtgc
     2101 ccgacgcctt gccattcctg ggttcggcca ccatcggctg cgcctatctg acgtgctcct
     2161 cgggcgcgta ccacccatgg ccggctgttg actttcagtt gccgctgggc ac
//
LOCUS       DASTFV010000638         3673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2431342, whole genome shotgun sequence.
ACCESSION   DASTFV010000638 DASTFV010000000
VERSION     DASTFV010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2431342"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VFP02_11690"
     CDS             complement(<1..604)
                     /locus_tag="VFP02_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9543742.1"
                     /translation="MAEVADPVVDRATGGAGGAAVAAVRLEGITKRFGSFTAVDDVDL
                     AVADGEFFSMLGPSGSGKTTCLRLIAGFETPTAGTVRLHGEDVNRRAPFERDVNTVFQ
                     DYALFPHMTVADNVGYGLLVRKVDRAERRRRVDETLALVQLGGLGGRKPAELSGGQRQ
                     RVALARALVVRPRLLLLDEPLGALDLKLRQAMQVELKAIQK"
     gene            689..2149
                     /locus_tag="VFP02_11695"
     CDS             689..2149
                     /locus_tag="VFP02_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HET9543743.1"
                     /translation="MLAVLRSTQGDDVAEQNFIGGQWVDAVGGATDAVIDPATGQEMA
                     RAASSDAADVDAAVQAAADAFVEWGASTPADRSAALLGLADVLEAEADALGALESQNV
                     GKPINTVGDEMAMAIDHIRFFAAGARNMDSRAAGEYLAGYTSMLRRDPLGVVASITPW
                     NYPLMMAVWKIGPALAAGNTVVLKPSELTPLTSLRMAELAAEVFPPGVLNVVTGQGET
                     AGAALVEHPLVRMVSLTGDVATGKLIAANAAPSLTRVHLELGGKAPVLVFDDADLEAV
                     IEAVAVGAYFNAGQDCTAACRVVAGPRLYDDLVAGLADAAAGIPQGDPSDESVDLGPV
                     VSEGQRTRVAGMVDNARAQGGEVVSGGVALDRDGWFYAPTVVAGVDQDAEIVQREVFG
                     PVVSVQRFADEEQAIAWANGVDYGLAASVWTRDVGRAMRLSKALQFGTVWVNDHIPLV
                     PEMPHGGFKQSGYGKDMSIYSIESYTELKHVMISHS"
     gene            complement(2214..3344)
                     /locus_tag="VFP02_11700"
     CDS             complement(2214..3344)
                     /locus_tag="VFP02_11700"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET9543744.1"
                     /translation="MTGAGVPALEGLKVIDCSTVLAGPGCARYLADFGADVIKVERPG
                     TGDTTRSLGWRDPRDGITLFWKLLGRGKRTIVLDLKSDEGRETMLALLDGADVLVENF
                     RPGTLERLGLGPDILLARNPDLVITRVTGFGQDGPYASRPGFATLAEAMSGLAAINGE
                     PDGAPLLPPIALADEVTGVVAAFATMVALHSGVGQVVDVSLLESLFQLMGPLPSLYAV
                     SGELQPRMGAQLPYTVPRNTYRCSDGTWVAVSTSAESVARRVLDLIGHGDDERLQSFE
                     GRVEHRVALDGWMADWIAERTAEEVIAAFEEAHAACAPVYDMADLAVDPHIAARETIV
                     DVDGTPMQGLIAKLSATPGRLRWSGRPLGADQAEVLAELDDD"
     gene            complement(3341..>3673)
                     /locus_tag="VFP02_11705"
     CDS             complement(3341..>3673)
                     /locus_tag="VFP02_11705"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HET9543745.1"
                     /translation="GLDRNQDLRVRIVRCARRHQAEVYGTVDLATRRRFPGAEVLRRQ
                     AATQCAQRFADYTGEPAGPGTEVSFAEVVPTLDSWSAGDRSALCVALGLDGASLRGSI
                     AADGGGSA"
BASE COUNT          515 a         1314 c         1354 g          490 t
ORIGIN      
        1 ccttctggat ggccttcagc tcgacctgca tggcctgacg cagcttgagg tccagggccc
       61 cgagcggctc gtcgagcagc aagaggcggg ggcgcacgac cagggcgcgg gcgagagcga
      121 cccgctggcg ctggccaccg gagagctcgg cgggcttgcg cccgcccagg ccgcccagtt
      181 ggaccagggc gagggtctcg tccacccggc ggcggcgctc ggcccggtcg accttgcgca
      241 ccaacaggcc gtagcccacg ttgtcggcga cggtcatgtg cgggaacagg gcgtagtcct
      301 ggaagacggt gttgacgtca cgctcgaagg gcgcccgccg gttgacgtcc tcgccgtgca
      361 ggcggaccgt gccggccgtc ggggtctcga agccggcgat gaggcgcagg caggtggtct
      421 tgcccgatcc ggacggcccc agcatcgaga agaactcgcc gtcggcgacg gcgagatcca
      481 cgtcgtcgac cgcggtgaac gagccgaagc gcttggtgat cccctcgaga cggaccgcgg
      541 cgacggcagc cccgcccgca ccgcccgtgg cccggtcgac gaccgggtcg gctacctcag
      601 ccacgttctc ccccctggct ctcggcccgt caagctagcg gaccctcttc gagcgggttg
      661 ccggggaccg ccgcccacca agggtcctat gctcgccgtc ctcagatcga cgcaggggga
      721 cgacgtggcc gagcagaact tcatcggggg tcagtgggtc gacgcggtgg gtggggcgac
      781 cgacgccgtg atcgacccgg ccaccggcca ggagatggcc cgggcggcgt ccagcgacgc
      841 ggccgacgtc gacgccgcgg tccaggccgc ggccgacgcc ttcgtcgaat ggggggcgtc
      901 cacgcccgcc gatcgcagcg ccgccctcct cggcctggcc gacgtgctcg aggccgaggc
      961 cgacgccctc ggtgccctcg agtcccagaa cgtgggcaag cccatcaaca ccgtgggcga
     1021 cgagatggcc atggccatcg accacatccg cttcttcgcc gccggggccc gcaacatgga
     1081 cagccgggct gccggggagt acctggccgg ctacacgtcg atgctgcgtc gggaccccct
     1141 gggtgtggtc gcctcgatca cgccgtggaa ctacccgctg atgatggccg tgtggaagat
     1201 cggccccgcc ctcgccgcgg gcaacaccgt ggtcctgaag ccgtccgagc tgaccccgct
     1261 gaccagcctg cgcatggccg agctggccgc cgaggtgttc ccgccgggcg tgctgaacgt
     1321 cgtcaccggt cagggcgaga cagccggcgc cgcgctggtg gagcacccgc tggtgcgcat
     1381 ggtgtcgctc accggtgacg tggccaccgg caagctcatc gccgccaatg ccgccccgtc
     1441 cctgacccgg gtccacctcg agctgggggg caaggctccc gtcctggtgt tcgacgacgc
     1501 cgatctcgag gcggtgatcg aagccgtcgc cgtcggggcc tacttcaacg ccggccagga
     1561 ctgcaccgcg gcctgccggg tcgtggccgg gccccgcctc tacgacgacc tcgtggccgg
     1621 cctggccgac gccgccgccg gcatccccca gggtgacccc tccgacgagt cggtcgacct
     1681 cggccccgtg gtctccgaag ggcagcggac ccgggtggcg ggcatggttg acaacgcccg
     1741 ggcccaaggg ggcgaggtgg tcagcggggg cgtcgccctg gaccgggacg gctggttcta
     1801 cgccccgacg gtggtggcgg gcgtggatca ggacgccgag atcgtccagc gcgaggtctt
     1861 cggccccgtc gtcagcgtcc agcgcttcgc cgacgaggag caggccatcg cctgggccaa
     1921 cggggtcgac tacggcctgg ccgccagcgt ctggacccgg gacgtcggcc gggccatgcg
     1981 cctgtccaag gccctgcagt tcggcacggt gtgggtgaac gaccacatcc cgctggtccc
     2041 ggagatgccc cacggcggct tcaagcagtc cggctacggc aaggacatgt cgatctacag
     2101 catcgagtcc tacaccgagc tcaagcacgt gatgatctcg cactcgtagg ccgcttcccg
     2161 ttctggcgcc tgattgcggg gtcatggcgc cgcaatcagg cgccagaacc gagtcaatcg
     2221 tcgtcgagct cggcgaggac ctcggcttgg tcggcgccca gggggcggcc ggaccagcgg
     2281 agccgaccgg gggtggcgct cagcttggcg atgaggccct gcatgggggt gccgtcgacg
     2341 tcgacgatgg tctcgcgggc ggcgatgtgc gggtcgacgg ccaggtcggc catgtcgtag
     2401 acgggggcac aggcggcgtg cgcctcctcg aaggcggcga tgacctcctc cgccgtgcgc
     2461 tcggcgatcc agtcggccat ccaaccgtcc agggcgaccc gatgctcgac ccggccctcg
     2521 aagctctgca accgctcgtc gtcgccgtgg ccgatgaggt ccaggacccg gcgggccacg
     2581 gactcggccg aggtggacac cgccacccac gtcccgtccg agcagcggta cgtgttgcgg
     2641 gggacggtgt acggcagctg ggcacccatg cggggctgga gctcgccgga gacggcgtag
     2701 agcgatggca gcgggcccat gagctggaac agggactcca gcaggctcac gtccacgacc
     2761 tggccgacgc cggagtggag cgcgaccatc gtggcgaagg cggcgaccac acccgtgacc
     2821 tcgtcggcca gggcgatggg cgggagcagc ggggcgccgt cgggctcccc gttgatggcc
     2881 gccaggccgg acatggcctc ggccagggtg gcgaacccgg gtcggctggc gtacggcccg
     2941 tcctggccga agccggtgac ccgggtgatg accaggtccg ggttgcgggc gagcaggatg
     3001 tcggggccga ggccgaggcg ttcgagggtg cccggccgga agttctcgac caacacgtcg
     3061 gcgccgtcga gcagggccag catcgtctcc cggccctcgt cgctcttgag gtccaggacg
     3121 atggtccgct tgccccgacc caacagcttc cagaacaggg tgatgccgtc ccggggatcc
     3181 cgccagccca acgaccgggt cgtgtccccc gtcccggggc gttcgacctt gatgacgtcg
     3241 gccccgaagt cggccaggta gcgagcgcag ccggggccgg ccaggacggt cgagcagtcg
     3301 atgaccttga gcccctcgag ggccggcacc ccggcgccgg tcacgccgac cctccgccgt
     3361 cggcggcgat gctgccccgc agcgaggcgc cgtcgaggcc gagcgccacg cacagcgcgc
     3421 tccggtcacc ggcgctccag gagtccagcg tgggcacgac ctcggcgaag gacacctcgg
     3481 tcccgggtcc ggcgggctct cccgtgtaat cggcgaagcg ctgggcgcac tgggtggccg
     3541 cctggcggcg caacacctcg gcccccggga agcgccgacg ggtggccagg tcgaccgtcc
     3601 cgtagacctc ggcctggtgg cggcgggcgc agcgcacgat ccgcacgcgc aggtcctggt
     3661 tgcggtccag gcc
//
LOCUS       DASTFV010000639         3709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2432583, whole genome shotgun sequence.
ACCESSION   DASTFV010000639 DASTFV010000000
VERSION     DASTFV010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2432583"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VFP02_11710"
     CDS             complement(<1..521)
                     /locus_tag="VFP02_11710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9543746.1"
                     /translation="MSRAAAAVARWSRGAAGAIRHPGRALDELTGPGPVYALLILFGL
                     NAVDELDRTAFGILLPEIREEFGLDLTSILALIAVVAAGALALQIPIAQYSDRHRRVP
                     VALGGAAVWAFFSLMTGLVSTVFFLGVARAGSGIGKAVVDPTHNSLLADYYPPVNRPG
                     VYSFHRAANALGQ"
     gene            complement(518..2632)
                     /locus_tag="VFP02_11715"
     CDS             complement(518..2632)
                     /locus_tag="VFP02_11715"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9543747.1"
                     /translation="MSTGGLDKAALREPLRRFGPALSVLAFQLLLFPVPTGVFVRGII
                     VGLLSALAALGLALVYRANRIINFAQTDLGSIPTTVGVYLIAFSGINYWLGLGAGLLS
                     AVVVGAVVELAIVRRFFDAPRLILTVATIGLATLLGACALLLPRLWGERPQAQALDLP
                     WDIRFTIEPLIFGADEVAALIVAPLVMIALAVFLRRTDVGVAVRASAERADRAALLGI
                     PVKRLQTVVWIVATLLAFSGAWLRAGVVGLPIGGSVSTFGFLLRILAALVIGRMVDLP
                     AVALAAIALGVLETGIDWNASSPLLTDPIVGAVIVVALLLQRRGAGRTDLESTSTWQA
                     AEEVRPIPTVVARLPEVRAVRWGGAVLVAAIVLWLPTWLSPSDSLLASSVGIYGIVGL
                     SLLVLTGWAGQVSLGQMGFVGLGAAIGGVATSQWGLDLSLALLVSGLAGAVAAVVVGL
                     PALRLQGLLLSVTTFAFALAMYSYFLNRQFFDWIPTGRIERNPIFGRIDYDSPTGVYY
                     LILVVLVLAILAVGGMRSSRFGRALIALRENEAGAQAFALRPLRLRITAFSLSGFLAA
                     LAGGLFVHHQQAFGPDTYAPFGGFLVFTMVVIGGLGSALGVLLGAAYLELTYRFAKDL
                     PGDVGIWRLLSNSLGVLLVLMILPSGIGGLVYRVRDGALRWTARRRGLVVPSFVEAAT
                     DEEPAILEAPVVTTDSLVEVDG"
     gene            2740..>3709
                     /locus_tag="VFP02_11720"
     CDS             2740..>3709
                     /locus_tag="VFP02_11720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET9543748.1"
                     /translation="MKVDGGLGAISTAGETARSLEAAGYDGAWSAETSHDPFLPHVLA
                     AEATEAIELGTGIAVAFGRNPMTLANTAWDLQMQSQGRFLLGLGSQIKPHITKRFSMP
                     WSSPAARMREMILAIRAIWDCWDGDGTLDFRGDFYTHTLMTPFFNPGPSPYGHPKVFV
                     AAVGPKMTEVAGEVADGMLCHGFTTASYLQEATLPSLETGLAKAGRTRADLELSIPAF
                     IVTGTSEEEMARSSIGVRRQVAFYGSTPAYRPVLDHHGWGDLQGELNRLSKQGEWHAM
                     GDLIDDEVLDAFAVVAEPHGVAEALLARWGDLVDRISFYAPYETDRD"
BASE COUNT          650 a         1268 c         1367 g          424 t
ORIGIN      
        1 aactggccca aggcgttggc cgcccggtgg aacgagtaga cgccagggcg gttgacgggt
       61 gggtagtagt cggccaacag cgagttgtgc gtcgggtcca ccaccgcttt gccgatgccc
      121 gagccggctc gggccacgcc gaggaagaag acggtggaca ccaggccggt catcagtgag
      181 aagaaggccc agaccgccgc cccgccgagc gccacaggta cccgtcggtg gcggtcggaa
      241 tactgcgcga tcgggatctg cagcgccagc gcgccggccg ccacgaccgc gatgagggcc
      301 aggatgctgg tcaggtccag tccgaactcc tcgcggatct cgggcagcag gatgccgaag
      361 gccgtgcggt ccaactcgtc gaccgcgttc aggccgaaga ggatcaggag ggcgtaaacg
      421 ggaccggggc cggtcagctc gtccaacgcc cgacccggat ggcggatcgc cccggcggcc
      481 ccccgggacc agcgggccac cgcggccgcg gccctgctca tccgtcgacc tccaccagcg
      541 agtcggtcgt gaccacagga gcttcgagga tggcaggctc ctcgtcggtc gcggcctcga
      601 cgaagctggg cacgaccagg ccccggcggc gggccgtcca gcgcagagcg ccgtcccgca
      661 cgcggtagac gaggccgccg atgccgctgg gcaggatcat gagcaccagg agcacgccca
      721 gggagttgga caggagacgc cagatcccga catcacccgg caggtccttc gcgaagcggt
      781 aggtcagctc caggtaggcg gcgccgagca ggacgcccaa ggccgagccc agaccgccga
      841 tgacgaccat tgtgaagacg aggaagccgc cgaagggggc gtaggtgtcc ggcccgaagg
      901 cctgctggtg gtggacgaac agccccccgg ccaaggcggc caggaagccg gagagcgaga
      961 acgcggtgat gcgcagccgc agggggcgga gggcgaaggc ctgggccccc gcttcgttct
     1021 cccgcagcgc gatgagcgcc cgaccgaagc gactggaccg catgcccccc accgcgagga
     1081 tggccaagac caggacgacc aggatcaggt agtagacgcc ggtcggcgag tcgtagtcga
     1141 tgcgtccgaa gatcgggttg cgctcgatcc gccccgtcgg gatccagtcg aagaactggc
     1201 ggttgaggaa gtacgagtac atggccaggg cgaaggcgaa ggtcgtgacg ctcaggagca
     1261 gaccctggag gcgcagggcg ggcagcccga ccaccacggc ggcgacggct ccggccaggc
     1321 cgctgacgag cagggccagg ctgagatcca ggccccactg ggacgtggcc acgccgccga
     1381 tcgccgcccc gaggccgacg aagcccatct gcccgagcga gacctggccg gcccacccgg
     1441 tgagcacgag gagggagagg ccgacgatgc cgtagatgcc gaccgacgag gccagcagcg
     1501 agtcgctggg tgagagccac gtgggcagcc acagcacgat ggcggcgacg aggacggcgc
     1561 ctccccagcg cacggcccgg acctccggca gccgagccac gaccgttggg atgggtcgca
     1621 cctcctcggc ggcctgccac gtcgacgtcg actcgaggtc cgtgcgtccc gcgccgcggc
     1681 gctgcagcag cagggcgacc acgatgacgg cccccacgat ggggtcggtg agcagggggc
     1741 tggaggcgtt ccagtcgatc ccggtctcca gcacgcccag ggcgatggcg gccagggcga
     1801 cggcgggcag gtcgaccatc ctcccgatca ccagggcggc caggatgcgg aggaggaacc
     1861 cgaaggtgga gacgctgccg ccgatgggca gcccgacgac gccggctcgg agccaggccc
     1921 cactgaacgc caagagggtg gccacgatcc agacgacggt ctggaggcgc ttgacgggta
     1981 tgcccagcag ggcggcccgg tcggcccgct cggccgacgc ccgcacggcc acgccgacgt
     2041 cggtccggcg caggaagacg gcgagggcga tcatcacgag gggggccacg atcagggcgg
     2101 ccacctcgtc ggcgccgaag atcagcggct cgatggtgaa gcggatgtcc cagggcaggt
     2161 cgagggcctg ggcctggggg cgctcccccc acagccgggg caggagcagg gcgcacgccc
     2221 cgaggagggt ggccagcccg atggtggcca cggtgaggat gagccggggg gcgtcgaaga
     2281 agcggcggac aatggccagc tcgacgacgg cgccgaccac caccgccgag aggaggccgg
     2341 cgccgaggcc caaccagtag ttgatcccgg agaaggcgat caggtacacg ccgacggtgg
     2401 tggggatgct gccgaggtcg gtctgggcga agttgatgat ccggttggcc cggtagacga
     2461 gtgccaggcc gagcgcggcc aacgcgctga ggaggccgac gatgatcccc cgcacgaaca
     2521 caccggtcgg aacggggaag agcaggagct ggaaggcgag gacggacagc gcgggcccga
     2581 aacggcgcag cggctcacgc agcgccgcct tgtcaagccc cccggtcgac atgtgtcgcg
     2641 caccgtactg cgcccgacgt accgcgtacg ttcggtgacc cgttccgggg gtcacccact
     2701 aggttcggcc gccaggatgc gaccacgggg agcacgacga tgaaggtcga cggaggcctg
     2761 ggggccatca gcacggcggg cgaaacggcc cgctccctgg aggcggcggg ctacgacggg
     2821 gcgtggagcg ccgagaccag ccatgacccg ttcctccccc acgtcctcgc cgccgaggca
     2881 accgaagcca tcgagctggg caccggcatc gccgtggcct tcgggcggaa ccccatgacg
     2941 ctggccaaca cggcctggga cctgcagatg cagtcccagg gccgcttcct gctcggcctc
     3001 gggtcccaga tcaagcccca catcaccaag cgcttctcga tgccctggtc cagcccggcc
     3061 gctcggatgc gggagatgat cctggccatc cgggccatct gggactgttg ggacggtgac
     3121 ggcacgctcg acttccgggg cgacttctac acccacaccc tgatgacccc gttcttcaac
     3181 ccggggccca gcccctatgg gcacccgaag gtgttcgtcg ccgcggtggg gcccaagatg
     3241 accgaggtgg cgggcgaagt ggccgacggg atgctgtgcc acggcttcac caccgcgtcc
     3301 tacctccagg aggccaccct gccgtcactg gagacgggct tggccaaggc cggtcggacc
     3361 cgggccgacc tcgagctctc catcccggcg ttcatcgtga ccgggacctc cgaggaggag
     3421 atggcccgat cgtcgatcgg cgtgcggcgc caggtcgcct tctacggttc gaccccggcg
     3481 taccgccccg tcctggacca ccacggctgg ggcgacctgc agggcgagct gaatcggctg
     3541 tcgaagcagg gtgagtggca cgccatgggt gacctgatcg acgacgaggt cctggacgcc
     3601 ttcgccgtcg tggccgagcc ccacggggtc gccgaggcac tcctggcccg ctggggtgac
     3661 ctggtcgacc gcatcagctt ctacgccccc tacgagaccg accgcgaca
//
LOCUS       DASTFV010000640         2619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2432929, whole genome shotgun sequence.
ACCESSION   DASTFV010000640 DASTFV010000000
VERSION     DASTFV010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2432929"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFP02_11725"
     CDS             <1..262
                     /locus_tag="VFP02_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543749.1"
                     /translation="LLPFAVGGVVALKRRGLPISPLVGMAVMISVTAAWTFGTTRYRI
                     PADVALVVAAAVGVEVVLRRRWPMTEPDPADDPDPEPVEAPA"
     gene            259..1179
                     /locus_tag="VFP02_11730"
     CDS             259..1179
                     /locus_tag="VFP02_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543750.1"
                     /translation="MNDNELNTELLNAELPDPRFVDAEYLAWLYDRNPDGPAIQAAAD
                     EDGVRVAHYALVPQTYRNAAGEAPFLFSLNAVTRRGTQRKGYWSELGLRNFAEATALG
                     RQLVIGVPNEKSTPGAVKYLSYRLLGPMPVKVAVPTGSGRGWDHHVVDEPFLDSATFT
                     ALAADLDRHDAVHWTNSYAPDRLAWRLARPHGHYVVHADDELVVVSTRSAFGPVPMAV
                     VLKLFPRGARSMDRPLSARRAIAAVCRYHRAPAAVYAGYNALVRVRGVQPPHRLRPAP
                     LNLVVHSFSDEVPQETFRLDTFEFLDLDAY"
     gene            1200..2024
                     /locus_tag="VFP02_11735"
     CDS             1200..2024
                     /locus_tag="VFP02_11735"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9543751.1"
                     /translation="MTPPFTFTFDVEDHRPDDGTELRLLDATRRVLAFCADRGVVGTV
                     FVVGEVVRDHPELVREIAAQGHELGLHGWRHTPITALTPEVFREETARGRALLAEVAG
                     QDVIGFRAPTFSLVPDTVWATDVLVDLGFTYSSSILPAWSPLYGYPGLPRHPFTWPSG
                     LLELPAPIIKAGPLGLPVVGGTYLRVLPWPVVRAGLRDKPLGSVPFTYCHPYDADPGE
                     PYWVVPGTGRLLSPLLWYGRKRMLRRIERLLAGEAGPPLRQRLADVPAEVPAVLAA"
     gene            complement(2031..>2619)
                     /locus_tag="VFP02_11740"
     CDS             complement(2031..>2619)
                     /locus_tag="VFP02_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543752.1"
                     /translation="FALGPDLPRNAADQAVWRAALDAHQPDLVVVSVGHWEYLEVLGD
                     FAEGELLEPGSYADEILDPFADLLTEEGARVLWVGPLVIDDAEEAEFVDGLQQDFHAL
                     ADRRDDVDFVDADTWVAPEGFSATLPGPDVEAVPVRRADGIHLCPEGQILLAQGLLEV
                     VAAELDLRPSPSWADDWRTEQEPEPGGCAPDYQGD"
BASE COUNT          336 a         1010 c          889 g          384 t
ORIGIN      
        1 cctcctcccg ttcgcggtgg gcggcgtggt cgccctcaag cggcggggcc tgcccatcag
       61 cccgctcgtg gggatggcgg tgatgatctc ggtcaccgcg gcgtggacgt tcggcacgac
      121 ccggtaccgg atcccggccg acgtggcttt ggtggtcgcc gccgcggtgg gcgtggaggt
      181 ggtgttgcgc cgacgctggc cgatgaccga acctgacccc gccgacgacc cggaccccga
      241 gcccgtggag gccccggcat gaacgacaac gagctgaaca ccgagttgtt gaacgcggag
      301 ctgcccgacc cccggttcgt cgacgccgag tacctggcct ggctctatga ccggaacccc
      361 gacgggccgg ccatccaggc cgcggccgac gaggacggcg tgcgggtggc ccactacgcc
      421 ctggtgcccc agacctaccg caacgccgcg ggggaggccc cgttcctgtt ctcgctcaac
      481 gccgtcaccc ggcggggcac ccagcgcaag gggtactggt ccgagctcgg gttgcggaac
      541 ttcgccgagg ccaccgccct gggtcggcag ctggtcatcg gcgtgcccaa cgagaagtcg
      601 acgccggggg ccgtcaagta cctgagctac cgcctcctcg ggcccatgcc ggtgaaagtg
      661 gccgtgccca ccggaagtgg ccgcggctgg gaccatcacg tggtcgacga gcccttcctc
      721 gacagcgcga cgttcaccgc cctggccgcc gacctggacc ggcacgacgc ggtccactgg
      781 accaacagct acgccccgga tcggctggcg tggcgcctgg cccgccccca cgggcactat
      841 gtcgtgcacg ccgatgacga gctcgtcgtg gtgtccaccc ggtcggcgtt cgggccggtg
      901 ccgatggccg tggtcctcaa gctcttcccc cgcggtgccc ggtcgatgga ccggccgctg
      961 tccgcccgac gggccattgc cgccgtgtgc cggtaccacc gggcccccgc cgcggtgtac
     1021 gccggttaca acgccctcgt ccgggtgcgg ggcgtccaac caccccaccg gctgcgcccg
     1081 gctcccctga acctggtcgt ccacagcttc agcgacgagg tcccccagga gacgttccgc
     1141 ctggatacct tcgagttcct cgacctcgac gcctactgag cgcccccgct ctccgagcca
     1201 tgaccccgcc cttcaccttc accttcgacg tcgaggacca ccgccccgat gacgggaccg
     1261 agctgcgcct cctggacgcc acccgccggg tgctggcctt ctgcgccgac cggggcgtcg
     1321 tgggcaccgt cttcgtggtc ggtgaggtcg tccgggacca ccccgagctg gtgcgcgaga
     1381 tcgcggccca gggccacgag ctcggcctcc acggatggcg acacaccccg atcaccgcgc
     1441 tgacccctga ggtgttccgc gaggagacgg cccggggtcg tgccctcctg gccgaggtgg
     1501 ccggccagga cgtgatcggc ttccgcgccc cgacgttctc tctcgtgccc gacaccgtct
     1561 gggccaccga cgtgctcgtc gacctcggct tcacctactc ctcgagcatc ctgcccgcct
     1621 ggagcccgct ctacggctac cccggcctgc cccgccaccc gttcacctgg ccgtccgggc
     1681 tcctggagct ccccgccccg atcatcaagg ccggtcccct cgggctcccg gtggtcggtg
     1741 gcacctacct gcgggtcctg ccctggcccg tggtgcgggc cggcctgcgg gacaagccgc
     1801 tggggtcggt gcccttcacc tactgccatc cctacgacgc cgacccgggt gagccgtact
     1861 gggtggtccc ggggaccggt cgtctgctca gccccctgct ctggtacggg cgcaagcgga
     1921 tgctgcgccg catcgaacgc ctgctggccg gcgaggccgg gccgccgctg cgccagcgcc
     1981 tggccgacgt gcccgccgag gtcccggccg ttctcgcggc ctgagggcga tcagtcgccc
     2041 tggtagtcgg gcgcgcagcc gcccggttcg ggctcctgct cggtccgcca gtcgtccgcc
     2101 cacgacggcg acgggcggag gtcgagttca gcggcgacga cctccaacaa cccctgggcc
     2161 agcaggatct gaccttcggg gcacaggtgg atgccgtccg cccgacggac cggaaccgcc
     2221 tcgacgtccg ggcccgggag ggtggcgctg aagccctcgg gcgcgaccca cgtgtcggca
     2281 tccacgaagt cgacatcgtc ccgccgatcg gccaaggcgt ggaagtcctg ctgcagcccg
     2341 tccacgaact cggcctcctc tgcgtcatcg atgacgaggg ggccgaccca aaggacgcgg
     2401 gcgccttcct cggtcagcag gtcggcgaag gggtccagaa tctcgtcggc gtagctcccg
     2461 ggctccagca gctcgccctc cgcgaagtcg ccgagcacct ccaagtactc ccagtgcccg
     2521 accgagacca cgaccaggtc gggctggtgc gcgtcgaggg cggcccgcca caccgcctgg
     2581 tcggctgcgt tgcgggggag atccgggccg agggcgaag
//
LOCUS       DASTFV010000641         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2434149, whole genome shotgun sequence.
ACCESSION   DASTFV010000641 DASTFV010000000
VERSION     DASTFV010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2434149"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..769
                     /locus_tag="VFP02_11745"
     CDS             86..769
                     /locus_tag="VFP02_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HET9543753.1"
                     /translation="MAEPLSPQVGWGVLHLFCKPTPTTDAQAVIAAVKVAEGEGDQVV
                     PVAVLGHKADLAFMVLGDELRRQRRFQTDLQAAGLEIVDSYVSLTEVSEYAQGMPEEM
                     KTPRLHPQLPPEGKTAWCFYPMSKKRVADQNWFTETYERRKELMLEHGTSGRKFAGRV
                     LQVITGSTGLDDFEWGVTLFAVHPDDLKEVVYTMRFDEASAVYAEFGPFFTGLVTPVE
                     DLVADLGLT"
     gene            882..2186
                     /locus_tag="VFP02_11750"
     CDS             882..2186
                     /locus_tag="VFP02_11750"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9543754.1"
                     /translation="MVLPAIVVACTMAVLAPHASAQDAGDDAATRGTAVSATLTYDVG
                     EGPDAVETPAVDVAFTVTGVGDEDIGDGVTDDDGVFVLEVPGPGEYGVTIDPSTVPDG
                     VELVDPDTLTRTVQVSPQETQRVLFALQEEGAVSSRDESSRFDQFLQLTVEGVKFGLV
                     LAMMAIGLSLIFGTTGLTNFSHGEIVVLGTVIVWYLNDWGIWLPLATLVGIILMFGVG
                     AGFDAGVWRPLRKRGTSLIAMLVISIGLSLLFRYLILYVFRGRPKAFHDYSVQVPYEF
                     GPVTIVPKDLWAIGLSIVVLTLVGLVLIGTRIGKAIRAVADNPDLARSSGIDVNRVIL
                     VVWGAGASLATLGGVLFATNELVDWQFGFKLLLLMFAGVTLGGLGTAFGAFVGSLIVG
                     IIIQVSTLWISPELKNVSALTLLILILVVRPQGIFGQRERIG"
     gene            2196..>2474
                     /locus_tag="VFP02_11755"
     CDS             2196..>2474
                     /locus_tag="VFP02_11755"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9543755.1"
                     /translation="MDWGDIISNALRAGVSREAAVFAIAAMGLNIQFGFTGLLNFGQV
                     GFMAIGAYGVGISITYYGWNPVVSLIVGILCCVVLALLLGLPALRLRAD"
BASE COUNT          348 a          863 c          837 g          426 t
ORIGIN      
        1 gggcacgaga cgggttgacg gcatctgtaa ggctaccttt acacagctgg ggccagagga
       61 gccagcccgc tcctacagtg gggccatggc cgaaccgctg agcccccagg tcgggtgggg
      121 cgtgctccac ctcttctgca agcccacgcc caccaccgac gcccaggccg tgatcgcggc
      181 ggtgaaggtg gccgaggggg agggcgacca ggtcgtcccc gtcgccgtcc tcggccacaa
      241 ggccgatctg gcgttcatgg tcctgggcga cgagctccgc cgccaacgcc gcttccagac
      301 cgacctgcag gccgccggcc tggagatcgt ggactcctac gtctccctca ccgaggtcag
      361 cgagtacgcc cagggcatgc cggaggagat gaagacgccc cgcctgcacc ctcagctccc
      421 tccagaaggc aagacggcgt ggtgcttcta cccgatgtcc aagaagcggg tggcggacca
      481 gaactggttc accgagacct acgagcgccg caaggagctc atgctggagc acggcacctc
      541 gggccggaag ttcgcgggcc gggtcctgca ggtcatcacc ggctcgaccg gcctcgacga
      601 cttcgagtgg ggcgtgaccc tgttcgccgt gcaccccgac gacctgaagg aggtcgtcta
      661 cacgatgcgt ttcgacgagg catcggccgt ctacgccgag ttcgggccct tcttcaccgg
      721 cctggtcacc cccgtcgagg acctggtcgc cgacctcggt ctcacctgaa cgtcaggtcg
      781 tcgtggtgta acttgcgcgc cgggggggtt ctctagactc ccgcctttcc accggaccgg
      841 tgggccgagg ggagacgaca gggacgttgc gcatccgggc ggtggtactc cccgcgatcg
      901 tcgtcgcctg caccatggcc gtcctggccc cccacgcatc ggctcaggac gccggggatg
      961 acgccgccac gcggggcacg gccgtctccg ccacgctcac ctacgacgtg ggcgagggcc
     1021 ccgatgcggt ggagacgccg gccgtggacg tcgcgttcac cgtgaccggc gtcggggacg
     1081 aggacatcgg cgacggcgtg accgacgacg acggggtgtt cgtgctcgag gtgccgggcc
     1141 cgggcgagta cggcgtgacc atcgacccct cgacggtgcc cgacggggtc gagctggtcg
     1201 atcccgacac gctgacgcgc accgtccagg tcagcccgca ggagacccaa cgggtgctct
     1261 tcgccctcca ggaggagggc gcggtcagct cccgggacga gtccagccgc ttcgaccagt
     1321 tcctccagct gacagtggag ggcgtgaagt tcggcctggt gctggccatg atggccatcg
     1381 ggctctcgct gatcttcggc acgacgggcc tcaccaactt ctcccacggc gagatcgtcg
     1441 tcctcggcac ggtcatcgtc tggtacctca acgactgggg gatctggctc ccgctggcga
     1501 cgttggtcgg catcatcttg atgttcggtg tcggtgccgg cttcgacgcg ggcgtctgga
     1561 ggccgctgcg caagcggggc accagcctca tcgccatgct ggtgatcagc atcggtctgt
     1621 ccctgctctt ccggtacctc atcctctacg tgttccgggg ccgcccgaag gcgttccacg
     1681 actactcggt gcaggtgccg tacgagttcg gccccgtcac catcgtgccc aaggacctct
     1741 gggccatcgg gctgtcgatc gtggtcctga ccctggtcgg cctggtcctc atcgggaccc
     1801 gcatcggcaa ggccatccgc gccgtggccg acaaccccga cctggcccgc tcgtcgggca
     1861 tcgacgtgaa ccgggtgatc ctcgtcgtct ggggcgccgg cgcctcgctg gccaccctgg
     1921 gcggggtcct cttcgccacc aacgagctgg tcgactggca gttcggcttc aagctgctgc
     1981 tgctgatgtt cgccggcgtc accctgggtg gcctgggcac ggcgttcggc gccttcgtgg
     2041 ggagcctgat cgtcggcatc atcatccagg tctcgaccct ctggatctcg cccgagctga
     2101 agaacgtgag cgccctgacc ctcctgatcc tgatcctggt cgtgcgcccc cagggcatct
     2161 tcggccagcg cgagcggatc ggctgagggg gcggcatgga ctggggcgac atcatctcga
     2221 acgcactgcg ggccggcgtc agccgggagg cggcggtgtt cgccatcgcg gccatggggc
     2281 tgaacatcca gttcggcttc accggcctgc tcaacttcgg ccaggtcggc ttcatggcca
     2341 tcggggccta cggcgtgggc atcagcatca cctactacgg ctggaacccg gtggtctcgc
     2401 tgatcgtcgg gatcctctgc tgtgtcgtgc tggccctcct gctgggcctg cccgccctgc
     2461 gcttgcgggc cgac
//
LOCUS       DASTFV010000642         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2437121, whole genome shotgun sequence.
ACCESSION   DASTFV010000642 DASTFV010000000
VERSION     DASTFV010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2437121"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VFP02_11760"
     CDS             <1..1030
                     /locus_tag="VFP02_11760"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9543756.1"
                     /translation="AFDRLAAKHASAAYRADEAAEIMSRKNFAATGSFVAEDRPRALD
                     LLGFQSQLVFNTFHNRRLRNWEHGGDGQLAIGAARAHNRGMVEFCAGDDRLLPTCYVP
                     LFDIDAAPDLVDEALTQGAAALLIASGCPPGHSPSHVGLDPVWAKAQDAGIPVVFHVG
                     GTGDLIDGNYFKNGLPIPPDFHGGEENFRSVDYMGIPHPPMQTLATMIFDGVLERFPD
                     LRFGVIEQGAIWVPSWLRQMESAYDAFHRHEERLQALSLRPTEYVRRQVRFTPYPTED
                     VGWIIANSGPEVCLFSSDYPHVEGGRKPLERFETSLGDADDATRQAFYCDNFLDLMGA
                     AGAQLAAA"
     gene            complement(1043..1492)
                     /locus_tag="VFP02_11765"
     CDS             complement(1043..1492)
                     /locus_tag="VFP02_11765"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HET9543757.1"
                     /translation="MKYFEDFVVGEVTDFPGRYLVTEEEIREVGERWDPQPFHVDPVA
                     AAESVFGGLVASSVHLFAISTCLGVKGEPTAAVSSLGFRAVNNHAPARPGDELRNRST
                     VLESRLSQSRPGVGVVTTLLELLNQRQELVFSWENAALIQCRPAPDP"
     gene            complement(1712..>2008)
                     /locus_tag="VFP02_11770"
     CDS             complement(1712..>2008)
                     /locus_tag="VFP02_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9543758.1"
                     /translation="LGLLRAVGTTRRQMRSMVRWESVLVASFGAVGGILVGSFLGWAL
                     VKAADADGFISAFSIPVGSLVVVLLVGAFAGALAAIRPARRAAKLDILDAIATS"
BASE COUNT          329 a          745 c          649 g          285 t
ORIGIN      
        1 cgccttcgac cggttggcgg ccaagcacgc ctcggcggcc taccgggccg acgaggcggc
       61 ggagatcatg agccgcaaga acttcgccgc caccggctcg ttcgtggccg aggaccggcc
      121 ccgggccctc gacctgctcg gcttccagag ccagctcgtc ttcaacacgt tccacaaccg
      181 gcggctgcgc aactgggagc acggcggtga cggccagctg gccatcggcg cggcccgggc
      241 ccacaaccgg ggcatggtcg agttctgcgc cggcgacgac cgactcctgc ccacctgtta
      301 cgtgccgctg ttcgacatcg acgccgcgcc cgacctggtc gacgaggcgc tgacccaggg
      361 cgcggcggcg ctgctcatcg cctcgggttg cccgcccggg cactccccca gccacgtcgg
      421 tctcgacccg gtgtgggcga aggcccagga tgcgggcatc ccggtcgtct tccacgtcgg
      481 cggaacgggc gatctgatcg acggcaacta cttcaagaac ggcctgccga tcccgcccga
      541 cttccacggc ggcgaggaga acttccgctc ggtcgactac atgggcatcc cccacccacc
      601 catgcagacg ctggcgacga tgatcttcga cggcgtgctg gaacggttcc cggatctccg
      661 cttcggcgtc atcgagcagg gcgccatctg ggtgccgtcg tggctgcgcc agatggagtc
      721 ggcctacgac gccttccacc gccacgagga gcgcctgcag gccctcagcc tgcgccccac
      781 cgagtacgtc cgccgccagg tccgcttcac cccgtacccc accgaggacg tgggctggat
      841 catcgccaac agcggccccg aggtctgcct gttctcctcg gactaccccc acgtcgaagg
      901 cggccggaag ccgctcgagc gcttcgagac gtccctcggc gacgccgacg acgccacccg
      961 ccaggccttc tactgcgaca acttcctcga cctcatgggc gcggctggcg cgcaactcgc
     1021 cgccgcctga ggcgttcagc ggctaagggt cgggcgccgg tcgacactgg atgagcgcgg
     1081 cgttctccca cgagaagacc aactcctgcc gctggttcag gagctccaag agcgtcgtga
     1141 cgacgccgac gccgggccgg ctctgcgaca gcctgctctc gagcaccgtg gaccgattcc
     1201 gcaactcgtc gcccggccgg gccggcgcat ggttgttgac ggcccggaag cccagcgagc
     1261 tcaccgccgc tgtcggctcg cccttgaccc ccaagcaggt ggagatggcg aagaggtgga
     1321 ccgaggacgc caccaacccg ccgaacaccg actcggccgc cgccaccggg tccacgtgga
     1381 agggctgcgg atcccatcgc tcgcccacct cccggatctc ctcctcggtc accaggtacc
     1441 ggcccgggaa gtcggtgacc tcaccaacca cgaagtcctc gaagtacttc accgcgctcc
     1501 tcccggagag agcggcgccg aacgtacacc tcgcgcccga ttcccgttcc gtctacagga
     1561 tctcgaatca gctcacgact tgcagtacac agaacgacga gaggccaacg ctggctggtt
     1621 gtattcagtt cggccagccc gacgcggttc tggcccttga tttcgttgcc atagcgcgga
     1681 gatcaagggc caggagccgg gttgggggga tctagctggt ggcgatggcg tcgaggatgt
     1741 cgagcttcgc ggcccgtctc gcggggcgga tggcggccag ggcgccggcg aaggcaccga
     1801 ccaggaggac gaccaccagc gagccgacgg ggatcgagaa ggcgctgatg aagccgtcgg
     1861 cgtcggcggc tttgaccagg gcccagccca ggaagctccc gaccaggatc ccgccgaccg
     1921 ccccgaagga ggcgaccaga accgactccc accgcaccat ggaacgcatc tggcgtcggg
     1981 tcgttcccac ggcccgcagc aggcccaa
//
LOCUS       DASTFV010000643         2270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2441846, whole genome shotgun sequence.
ACCESSION   DASTFV010000643 DASTFV010000000
VERSION     DASTFV010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2441846"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VFP02_11775"
     CDS             complement(<1..286)
                     /locus_tag="VFP02_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9543759.1"
                     /translation="MAPPTDAASINDRRWAVLAVMNLSLVIVVAGNSALNVALPSIVR
                     DLDATSSQLQWMVDAYALVFAGLLLPMGALGDRFGRKGMLQGGLSIFALGS"
     gene            complement(388..1008)
                     /locus_tag="VFP02_11780"
     CDS             complement(388..1008)
                     /locus_tag="VFP02_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543760.1"
                     /translation="MARLAAGETSAAFVLAMDFSAPIGAAVRSHLADLGVHDVDRDEL
                     DSLVIDVCTMLAGIAGAWRADGGAAPWQWAYHRVRQVVSGFVGQHADALDEALLDLPA
                     PAAAPGAEPDLVDLLDGLCAEWPLVALVREGLEVVASARDRAILLEVEVQAAMGDPSP
                     ALTIAVERGMQPAAVRQVVSRTRRRLQALAAGDERFAPLAELALAA"
     gene            complement(1164..1697)
                     /locus_tag="VFP02_11785"
     CDS             complement(1164..1697)
                     /locus_tag="VFP02_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543761.1"
                     /translation="MSRTTRVGPVDGFWATVDEWAAEVVDPSRVRAWWTSRCRLRAVA
                     GPYLDVTGDAEALDRRLTPLRDPVANRGIAVADDDALRSALVDAGVPFRVVPAARRYR
                     WNGPMLTPVVLELHVSGDEATLQAWWEDVWASWPTIRLGRESTAQRPPRRATPIAQIT
                     RLFAALGHEPLQRQDNV"
     gene            1759..>2270
                     /locus_tag="VFP02_11790"
     CDS             1759..>2270
                     /locus_tag="VFP02_11790"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HET9543762.1"
                     /translation="MTDENRFRDRPKVVLHDHLDGGVRPATVVDLAAAQGVELPVAEA
                     ELADWFTLRPGMPFPEAWSRFHLVISLLQDADSLRRVAREAVEDVAADGVVYLETRFG
                     PLSHQRSGLGSDEVMAAVIAGLAEGEAATGTVARLIVCGIREDGPALGLEAARLAVAW
                     GDRGVVGYDIA"
BASE COUNT          311 a          803 c          871 g          285 t
ORIGIN      
        1 gcgagcccag ggcgaagatc gagaggccgc cctggagcat gcccttgcgc ccgaagcggt
       61 ccccgagggc gcccatgggg agcagcaggc cggcgaagac gagggcgtag gcgtcgacca
      121 tccactgcag ctgtgagctg gtggcgtcga ggtcgcggac gatggagggc agggccacgt
      181 tcagggccga gttgccggcc acgacgatga ccaggctgag gttcatcacg gccaggaccg
      241 cccagcggcg gtcgttgatg gatgcggcgt cggtgggtgg ggccacgggg ggtgcctcct
      301 gaaggcggat cgcgagctat cgctacgcta cggtagcgta gcgaatctgt ccagggggat
      361 cgccgtgaca tcgggcacag ccgcgggtca ggcggccaaa gcgagctccg ccagcggggc
      421 gaagcgctcg tcgccggcgg ccagggcctg caggcggcgc cgtgtccgcg acaccacctg
      481 gcgcaccgcg gcgggctgca tgccccgctc gacggcgatg gtgagcgcgg gggaggggtc
      541 ccccatggcc gcctggacct cgacctcgag caggatcgcc cggtcgcggg cgctggccac
      601 gacctcgaga ccctcgcgga ccagcgccac caggggccac tcggcgcaga ggccgtcgag
      661 gagatccacc aggtccggct cggcgcccgg cgccgcggcc ggcgccggaa ggtcgagcag
      721 ggcctcgtcc agggcgtcgg cgtgctggcc gacgaacccg ctgaccacct gacggacccg
      781 gtggtaggcc cactgccagg gcgcggcccc gccgtcggcc cgccaggccc cggcgatgcc
      841 ggccagcatc gtgcacacgt cgatgaccaa cgagtccagt tcgtcccggt cgacgtcgtg
      901 gacccccaga tcggcgaggt gcgaacgcac cgccgccccg atgggcgcgc tgaagtccat
      961 ggccaggacg aacgccgcgc tcgtctcgcc ggccgccaac cgcgccatga gcatttccaa
     1021 ccgaactcgc ctgtccctct ccatggggtc atcctcccca gggggtgtga cagtcaaact
     1081 cgactggctt cacttcgttc agcaagtccg gctccgccga agctgcggat cggcgatgct
     1141 gctccgctcc gactgcgcag cgtctacacg ttgtcctggc gttggagcgg ttcgtggccg
     1201 agggcggcga agaggcgcgt gatctgggcg atgggggtgg cccggcgagg ggggcgctgg
     1261 gcggtggact cgcggccgag gcggatggtg ggccacgacg cccagacgtc ctcccaccag
     1321 gcctggaggg tggcctcgtc gccgctgacg tggagctcca agaccacggg ggtgagcatc
     1381 gggccgttcc agcggtagcg gcgggcggcg gggacgaccc ggaacgggac tccggcgtcg
     1441 accagggcgg agcggagggc gtcgtcgtcg gccacggcga tcccccggtt ggcgacgggg
     1501 tcgcggaggg gcgtcagccg gcggtcgagc gcctccgcgt caccggtcac gtcgaggtag
     1561 gggccggcca ctgcccggag acggcagcgg ctcgtccacc acgcccgcac ccgcgacggg
     1621 tcgaccacct ccgccgccca ctcgtcgacg gtcgcccaga aaccgtcgac gggcccgacg
     1681 cgggtggtgc ggctcacgcc ccccattgca caccgtgggc ggtccggcct gcgaagtggg
     1741 tcctaggctc cgggcccgat gaccgacgag aaccggttcc gcgaccggcc caaggtggtc
     1801 ctgcacgacc acctcgacgg cggcgtacgg ccggccaccg tggtcgacct ggccgcggcc
     1861 caaggggtcg agctgccggt ggccgaggcc gagctggccg actggttcac gctccgcccg
     1921 ggcatgccgt tccccgaggc ctggtcccgc ttccacctgg tcatctcgct gctgcaggac
     1981 gccgactcgc tgcgccgagt ggcccgcgag gcggtggagg acgtggccgc cgacggcgtc
     2041 gtctacctgg agacccgttt cggccccctc agccaccaac ggtcgggcct gggtagcgac
     2101 gaggtgatgg ccgccgtcat cgccggcctg gccgaggggg aggcggccac gggcaccgtc
     2161 gcccgcctga tcgtctgcgg gatccgggag gacggccccg ccctcgggct ggaggcggcc
     2221 cggctggccg tggcctgggg cgaccggggc gtggtgggct acgacatcgc
//
LOCUS       DASTFV010000644         3394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2443628, whole genome shotgun sequence.
ACCESSION   DASTFV010000644 DASTFV010000000
VERSION     DASTFV010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2443628"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VFP02_11795"
     CDS             complement(<1..590)
                     /locus_tag="VFP02_11795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HET9543763.1"
                     /translation="MHIEFNGSPAPTLGVEVELELVDHETGELVSAAADILSELGQGH
                     PEGEHPKAKHELFECTIEIITGICDTVAEARADLSATLAEVREAADRRGIDLLCSGTH
                     PFSTWGDQKVSDDPRYRLLLEQMGWTAQRLQIFGVHFHVGVRHGERVLAIANGLAGYI
                     PHLLALSASSPYWESRDTGLASSRSKVFEALPTAGLP"
     gene            complement(614..1681)
                     /gene="ruvB"
                     /locus_tag="VFP02_11800"
     CDS             complement(614..1681)
                     /gene="ruvB"
                     /locus_tag="VFP02_11800"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET9543764.1"
                     /translation="MAEEPTGAVRDEILVPQETVADVVDEVTDDDPGLRPRTLDEFVG
                     QAEIKEHLSIVLEAAAQRGQPADHLLLAGPPGLGKTTLAGIVAHEMGVGLQITSGPAL
                     ERAGDLAAILTRLETGDVLFIDEIHRLPRAIEEVLYPAMEDFELDIVLGKGPAARSIR
                     LELPPFTLVGATTRTGLITGPLRDRFGLVARLEFYDPEDLESIVVRTAGILGIQIDQP
                     GSAEIAGRARGTPRIANRLLRRVRDFAEVRAGGRIDLEAAGDGLAAFGVDERGLDKVD
                     RSILSALCQSFGGGPVGLKTLAIAVSEPSETVEDVYEPFLIQQGLLMRTPRGRVATPA
                     AWSHLGLAPPSAPPGAASLFE"
     gene            complement(1687..2280)
                     /gene="ruvA"
                     /locus_tag="VFP02_11805"
     CDS             complement(1687..2280)
                     /gene="ruvA"
                     /locus_tag="VFP02_11805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET9543765.1"
                     /translation="MIGSLRGILLDRSLDGDVLIEVAGVGYRVTVAPTASPAVGEIGD
                     DVFLWVHHHIREDAQTLYGFATREERVCFEALLGAHGVGPALALAILSVHGPGALREV
                     IATQDVSALCLVPGVGNKTAARLLVELQSRLDLPDIDLTPSPGAVVTTSARRDVTEAL
                     AGLGYGPDEVRRVVDDLPSDGEPADLLREALRRLAVA"
     gene            complement(2277..2768)
                     /gene="ruvC"
                     /locus_tag="VFP02_11810"
     CDS             complement(2277..2768)
                     /gene="ruvC"
                     /locus_tag="VFP02_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET9543766.1"
                     /translation="MFVLGVDPGLTRCGYGCVEQVGRAPRAVALGVLRTDKDDPLPKR
                     LADLQVGIRALIAELEPAVVAVERVFFQVNVRTAMAVGQASGIVMAEAHAAGCEVEMY
                     TPNQVKEAVAGHGSADKEQVGQMVQTLLGLAAPPAPADVADAAALALCHLAHAPMKAK
                     VRA"
     gene            2846..>3394
                     /locus_tag="VFP02_11815"
     CDS             2846..>3394
                     /locus_tag="VFP02_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9543767.1"
                     /translation="MDAPVVAARALGRRFGEVDALSDLNLSVPEGQITVLLGPNGAGK
                     TTAIRMITGALNPDRGEIRTFGVDPGDEGEDVRRRCGVVSAKPALYDRLNGWDNLAYA
                     AELYGLRDYDERLREAAARFGIEAALQKMVGGYSTGMKTRLALARSIMHDPDLLLYDE
                     PTSGLDPESSHAVLELIRSMTED"
BASE COUNT          464 a         1185 c         1283 g          462 t
ORIGIN      
        1 ggcaggccgg cggtgggcag ggcctcgaag accttgctgc ggctggaggc caggccggtg
       61 tcgcggctct cccagtaggg gctggacgcg gagagggcca gcaggtgtgg gatgtagccg
      121 gccaggccgt tggcgatggc cagcacccgc tcgccgtggc ggacgccgac gtggaagtgg
      181 accccgaaga tctgcaggcg ctgggccgtc caccccatct gttccagcaa caggcggtag
      241 cgggggtcgt cgctgacctt ctggtcgccc caggtggaga acggatgggt gccggagcag
      301 agcaggtcga tgcctcggcg gtcggccgcc tcccgcacct cggccaaggt ggcgctcagg
      361 tcggcccggg cctcagccac cgtgtcacag atgcccgtga tgatctcgat cgtgcactcg
      421 aagagctcgt gcttggcctt cgggtgctcc ccctcggggt ggccctgccc cagctcgctc
      481 aggatgtcgg cggcggcgct gacgagctcg ccggtctcgt ggtcgaccag ctccagctcg
      541 acctcgacgc cgagggtggg cgcgggcgac ccgttgaact cgatgtgcac cctcggagcg
      601 tagaggcgac cggctactcg aacagtgagg cggcgcccgg cggcgcgctc ggaggggcca
      661 ggccgaggtg cgaccaggcc gccggcgtgg ccacccggcc ccggggcgtg cgcatgagca
      721 ggccctgttg gatcaggaac ggctcgtaga cgtcctccac ggtctcggag ggctcgctga
      781 cggcgatggc cagcgtcttg aggcccacgg ggccgccgcc gaagctctgg cagagggccg
      841 acaggatgga gcggtcgacc ttgtccaggc cccgctcgtc cacgccgaac gcggccaggc
      901 cgtcgcccgc ggcctcgagg tcgatccggc cgccggcccg gacctcggcg aagtcgcgga
      961 cccggcgcag gagccggttg gcgatgcggg gcgtgccccg ggcccggccc gcgatctcgg
     1021 cgctgccggg ctggtcgatc tggatgccga ggatcccggc ggtgcgcacc acgatcgact
     1081 cgaggtcctc cgggtcgtag aactcgaggc gggccacgag gccgaagcgg tcccgcaacg
     1141 ggccggtgat gaggccggtg cgggtggtgg ccccgaccag ggtgaagggc ggcagctcga
     1201 ggcggatgga gcgggcggcc ggccccttgc ccagcacgat gtccagctcg aagtcctcca
     1261 tggccgggta gaggacctct tcgatggctc ggggcagccg gtggatctcg tcgatgaaga
     1321 gcacgtcgcc ggtctcgagc cgggtcagga tggcggccag gtcgccggcc cgctcgaggg
     1381 cgggacccga ggtgatctgg aggccgacgc ccatctcgtg ggcgacgatg ccggccaggg
     1441 tggtcttgcc caggcccggc ggaccggcca gcagcaggtg gtcggcgggc tggccccgtt
     1501 gggccgccgc ctccaggacg atcgagaggt gctccttgat ctcggcctgt ccgacgaact
     1561 cgtccagcgt gcgggggcgc aggcccgggt cgtcgtcggt gacctcgtcc accacgtcgg
     1621 ccacggtctc ctgcgggacg aggatctcgt cccggacggc gcctgtgggc tcctcggcca
     1681 tgcggctcag gctacggcga ggcggcgcag cgcttcgcgg agcaggtcgg cgggctcgcc
     1741 gtcggagggc aggtcgtcga ccacccgccg gacctcgtcg gggccgtacc ccaggccggc
     1801 gagggcctcg gtgacgtccc ggcgggcgga ggtggtcacg accgcaccgg gcgagggggt
     1861 gaggtcgatg tcgggcaggt cgaggcggga ctggagctcg accagcaggc gggccgcggt
     1921 cttgttgccc accccgggga cgaggcagag ggcactgacg tcctgggtgg cgatgacctc
     1981 gcgcagggcg ccagggccgt ggaccgagag gatggcgagg gccagcgccg ggccgacgcc
     2041 gtgggccccg agcagggcct cgaagcagac ccgctcctcc cgggtggcga agccgtagag
     2101 ggtctgcgcg tcctcccgga tgtggtggtg gacccacagg aagacgtcgt cgccgatctc
     2161 gccgaccgcg ggcgaggcgg tcggcgccac cgtcacccgg tagccgaccc cggccacctc
     2221 gatgaggacg tcgccgtcca gggagcggtc gagcaggatg ccccgcagcg agccgatcac
     2281 gcccgtacct tcgccttcat cggggcgtgg gccaggtggc acagggccag ggcggcggcg
     2341 tcggccacgt cggccggcgc gggcggggcg gccagcccca ggagcgtctg caccatctgt
     2401 cccacctgct ccttgtctgc gctgccgtgg ccggcgacgg cctctttgac ctggttcggg
     2461 gtgtacatct cgacctcaca gccggcggcg tgggcctcgg ccatgacgat gccactggcc
     2521 tgcccgacgg ccatggccgt gcgcacgttg acctggaaga acacccgctc gacggcgacc
     2581 acggccggct cgagctcggc gatgagagcc cggatcccga cctgcaggtc cgccagccgc
     2641 ttcggcaggg gatcgtcctt gtcggtgcgc agcacgccca acgccacggc ccgcggcgca
     2701 cgcccgacct gctccacgca gccgtagcca caccgggtca accccgggtc gacacccaac
     2761 acgaacatct gtacgaggtt aggtcgatcg cctcccgctc ccgcggatgc tcacgggtga
     2821 gcgcggacct gccgatgggc ggggcgtgga tgctcctgtc gtcgctgctc gcgcgctcgg
     2881 tcgacgcttc ggcgaggtcg atgcgctgtc cgacctgaac ctgtcggtgc ccgagggtca
     2941 gatcaccgtc ctgctcgggc ccaacggggc gggcaagacc acggccatcc gcatgatcac
     3001 cggcgcgctg aaccccgacc ggggtgagat ccggacgttc ggcgtcgatc ccggcgacga
     3061 gggcgaggac gtccgccggc gctgcggtgt cgtgtcggcc aagccggcgc tgtacgaccg
     3121 actgaacggc tgggacaacc tggcctacgc cgccgagctc tacggcctgc gggactacga
     3181 cgagcgcctc cgggaagcgg cggcccgctt cggcatcgaa gcagccctcc agaagatggt
     3241 cgggggctac tcgacgggca tgaagacccg cctggccctg gcccgttcga tcatgcacga
     3301 ccccgacctg ctgctctacg acgagcccac ctcgggcctg gaccccgagt cctcccacgc
     3361 cgtgctcgag ctcatccggt ccatgaccga ggac
//
LOCUS       DASTFV010000645         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2444035, whole genome shotgun sequence.
ACCESSION   DASTFV010000645 DASTFV010000000
VERSION     DASTFV010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2444035"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="VFP02_11820"
     CDS             complement(<1..1169)
                     /locus_tag="VFP02_11820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9543768.1"
                     /translation="MRALPDGLSVREATLEDHDGIIAVCTEVFDGYEGATVRHLLTHP
                     GYGPGRWTVVVDADGAVVSTCVLLTHRLRYGQTEVPAVQIEFVATREHARKKGLVRAQ
                     FDLHHRWAADQGALVLLITGIPYLYRRLGYGYGFAYAPQHRLLAAPKAPDGWTVEPAT
                     AADLPEIERLQVQSLDRFDVALCWPETGWSWLLEGAPTWEELVMVARQGDTVGGFARV
                     QLRPEENYAVAEGAADAIAPAQALLAETAARTTDVRLFLLERVGDPWGTVVRAAGASD
                     PAWFNTVYARIPDPVAFLDHVRPELSARLAASPYATERGELALSFYEDGVILTYADGV
                     VTDVRQDPDPALDAMDDDKAGIAPDAVPALFLGRFGARELEVRQDDVGYVADRDLV"
     gene            complement(1180..1893)
                     /locus_tag="VFP02_11825"
     CDS             complement(1180..1893)
                     /locus_tag="VFP02_11825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9543769.1"
                     /translation="MATNEAEVVFPHYYALLDRLQEHLAPRGYIEIGVADGHSLGLTR
                     PGTIAVGVDPAPGPEVAERAPGRRVVTTTSEAFFADHERADVLGDAPLDLAFVDGLHL
                     FEAALADVAALEAWAHPGTVVLLHDCHPTNAAMAARERTTVAWAGDVWKVLACLRADR
                     PDLTVTTVDVGPTGLGVITGLGAGFVDLDDSIRRHRDLDYSVLEADPGGLLGLVPPTW
                     TAVEPLLPDNSRASGATAS"
     gene            complement(1895..2092)
                     /locus_tag="VFP02_11830"
     CDS             complement(1895..2092)
                     /locus_tag="VFP02_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543770.1"
                     /translation="MDSGDLIRDAFELLIVVALGGMVWSVLTRLRRGQLRVPTCPECG
                     GPASRAYPKCRHCGAVQPEGR"
     gene            complement(2111..2473)
                     /locus_tag="VFP02_11835"
     CDS             complement(2111..2473)
                     /locus_tag="VFP02_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543771.1"
                     /translation="METRRWTNPSQPQTLQIAVFLLYANAVLGVLLPQLPLLTLAGLA
                     GAAGAYGIANSRRWGYQVAVTASAFEVAVVAVLPLLDNGLETLFNLRFLIYILFPVAL
                     FCLLVHPDSRQHQRVWFE"
     gene            2472..>2933
                     /locus_tag="VFP02_11840"
     CDS             2472..>2933
                     /locus_tag="VFP02_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9543772.1"
                     /translation="MSTSVAGIVLAAGAGTRLAPLTRQRPKALCPVGGTALVDLAEAR
                     VRAVTETVAVNVHHGREALLAHLGDRVHVSLEDPEPLGTAGAVGALRDWLDGRPAVIV
                     NADGWCGGGLDRLLEGWDGERVRILLPGGGDFKPQSMIAGSLLPWALAQTIE"
BASE COUNT          437 a          949 c         1169 g          378 t
ORIGIN      
        1 accaggtccc ggtcggcgac gtagccgacg tcgtcttggc ggacctcgag ctcgcgggcg
       61 ccgaaccgac cgaggaacag cgcgggcacg gcgtccgggg cgatgccggc cttgtcgtca
      121 tccatcgcgt ccaacgccgg gtcggggtcc tggcgcacgt cggtgaccac accgtccgcg
      181 tacgtgagga tcacgccgtc ctcgtagaac gacagggcca actcgcctcg ctcggtggcg
      241 tagggggagg cggccaagcg agccgacagc tcggggcgga cgtggtccag gaaggccacc
      301 gggtcgggga tgcgggcgta gaccgtgttg aaccatgccg ggtcggaggc cccggcagcc
      361 cgcacgacgg tgccccacgg gtcgccgacg cgctcgagca ggaagaggcg gacgtcagtg
      421 gtacgggcgg cggtctccgc caacagggcc tgggccgggg cgatggcgtc ggccgcgcct
      481 tccgcgaccg cgtagttctc ctcgggccgg agttggacac gggcgaagcc gccgaccgtg
      541 tcgccctgtc gggcgaccat gaccagctcc tcccaagtcg gggcgccctc caggagccag
      601 gaccagcccg tctcgggcca gcacagggcg acgtcgaacc ggtccaggga ctggacctgc
      661 agacgctcga tctcggggag gtcggctgcg gtggccggct cgacggtcca gccgtcgggc
      721 gccttcgggg ccgccaggag ccggtgttga ggagcgtagg cgaagccata gccgtagccg
      781 agtcgccggt acaggtacgg gatgccggtg atgaggagca cgagggctcc ctgatccgcc
      841 gcccaccggt ggtggaggtc gaactgggcc cgaacgaggc ctttcttgcg ggcgtgctcc
      901 cgggtcgcca cgaactcgat ctggacggcg gggacctcgg tctggccgta gcggaggcga
      961 tgggtgagga gcacgcaagt cgagacgacc gcaccgtcgg catcgacgac cacggtccac
     1021 cggccgggcc cgtaaccggg gtgggtgagc agatgacgga cggtggcgcc ttcgtagccg
     1081 tcgaagacct ccgtgcagac ggcgatgatc ccgtcgtggt cctcgagggt ggcctcgcgg
     1141 acgctcagtc cgtcgggcag ggcacgcatg gaccggacgc tacgaggctg tcgcccccga
     1201 ggcccgggaa ttgtccggca gcaagggctc gacggcagtc cacgtcggcg gcaccagtcc
     1261 gaggaggccg cccgggtccg cttcgagcac cgagtagtcg aggtcgcggt ggcgacggat
     1321 cgaatcgtcc aggtcgacga acccggcccc caggccggtg atcacgccga ggccggttgg
     1381 gccgacgtcc acggtggtga cggtgaggtc ggggcggtcg gcccgcaggc aggccaggac
     1441 cttccagacg tcgccggccc aggcgaccgt ggtgcgctcg cgggcggcca tggcggcgtt
     1501 ggtcgggtgg cagtcgtgaa ggagcacgac ggtgccggga tgggcccagg cctcgagggc
     1561 ggccacgtcg gccagggccg cctcgaacag gtggaggccg tcgacgaagg ccaggtcgag
     1621 cggagcgtcg ccgagcacgt cggcccgctc gtgatcggcg aagaaggcct cgctggtggt
     1681 ggtcacgacc cgccggccgg gcgcccgctc ggccacctca gggcccgggg ccgggtcgac
     1741 cccgacggcg atcgtgcccg gccgggtgag gccgaggctg tggccgtccg ccacgccgat
     1801 ctcgatgtag ccccgggggg ccaggtgctc ctggagacgg tcgagcaggg cgtagtagtg
     1861 ggggaagacg acctcggcct cgttcgtggc cacgtcagcg gccctcaggc tgcacggcgc
     1921 cgcagtgacg gcacttcggg tacgcccggg aggcgggacc gccgcactcc gggcacgtgg
     1981 ggactctgag ctggccccga cggagccggg tcaggaccga ccagaccatg cccccgaggg
     2041 cgacgacgat gagcaactcg aaggcgtcgc ggatgaggtc gccggagtcc acggcctcag
     2101 tgtggcggga tcactcgaac cagacgcgct ggtgctggcg gctgtcgggg tgcacgagca
     2161 ggcagaacag ggcgaccggg aagaggatgt agatcaggaa gcgcaggttg aagagggtct
     2221 cgaggccgtt gtcgagcagg ggcaggacgg cgacgaccgc cacctcgaag gcggaggcgg
     2281 tcaccgcgac ctgatagccc catcggcggc tgttggcgat gccgtacgct ccggctgcgc
     2341 cggcgaggcc ggcgagggtc agcagcggca gttggggcag cagcacgccc agcacggcgt
     2401 tggcgtagag caggaacacc gcgatctgga gggtctgggg ctgtgagggg ttggtccagc
     2461 ggcgggtctc catgagcacc agtgtggcag ggatcgtcct ggctgcaggg gccgggaccc
     2521 gtttggcccc gctgacccgg cagcggccca aggcgctctg cccggtcggg gggacagccc
     2581 tcgtggacct ggccgaggcc cgagtccggg cggtcaccga gacggtggcc gtgaacgtcc
     2641 accacggacg agaggctttg ctggcccacc tcggggaccg ggtgcacgtc tcgctggagg
     2701 atcccgagcc actcggtacg gcgggggccg tcggggcgct ccgcgactgg ctcgacgggc
     2761 gaccggctgt gatcgtcaat gccgacggct ggtgcggggg aggcctggac cgcctgctcg
     2821 agggttggga cggcgaacgg gtgcggatcc tgctaccggg cgggggcgac ttcaagcccc
     2881 agtcgatgat cgcggggtcg ctcctgccgt gggctctggc ccagacgatc gag
//
LOCUS       DASTFV010000646         3167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2446798, whole genome shotgun sequence.
ACCESSION   DASTFV010000646 DASTFV010000000
VERSION     DASTFV010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2446798"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3167)
                     /gene="dnaE"
                     /locus_tag="VFP02_11845"
     CDS             complement(<1..>3167)
                     /gene="dnaE"
                     /locus_tag="VFP02_11845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET9543773.1"
                     /translation="FLEGYFYKPRIDWELLDEHSEGLIATTACLGGHVLQALMTGDEA
                     EALTRAGRLQDIFGKDNLFVEIQDHGIDRQRQTMPGLLAISRKLGAPLLATNDSHYVA
                     REDAKAHDALLCVQTNSMMSDPTRFKFDGEEHYLKSADEMRRLFPETDYPGACDNTLL
                     IAERCNVEIEFGKPLLPRFPLPDGFTEDSAYLKHLTLEGAKARWGQQLPDAVLERVAF
                     ELQVIEDMGFSSYFLIVWDLIKHARDKGIRVGPGRGSAAGCCVAYCLRITDLDPIKYD
                     LLFERFLNPSRISMPDIDMDFDSRFRDEMIRYAAERYGRDRVAQIVTFSTIKARAAVR
                     DAARVLGYPYALGDKVAKLMPPLIMGRDTPLHACLEKTEKFEEGYKMAGELRTVYESD
                     SDARAVIDVATGLENLRRQDSIHAAAVVITDQPVTEYAPVQRKPESGQAPEDAPIVTQ
                     YEMNGIESLGLLKMDFLGLRNLDVIEDTLELIRDVRGEDVDIDNVPLDDPKTLAMLCR
                     GESIGVFQLEGGPMRALMRSLAPDSFDDIAALVALYRPGPMGANMHNDYADRKNGRKP
                     VTYLHPDLEETLGDTYGLMIYQESVMRVAQKVAGYSLAEADNLRKACGKKIREMIRAE
                     RDKFVAGCETTGYGRDLGDQLFDIIEPFADYAFNKSHAYGYGLIAYQTAWLKAHYPVE
                     YLAALLTSVKDSQDKAAIYLAECRTMGIEVRVPDINLSVSKFTAVPPEDVTAGTLGSI
                     PFGLSAVRNVGEGVTDLILAQREAGGPFVDFYDFCERVDTAALNKRTVEALIKSGAFD
                     LLGHTRKGLLAAYERIIDLTVAKRKQHDMGAMSLFDSIDDGPVFDDRPRIDEGELDKK
                     LKLAFEKEMLGLYISDHPLMGVESSLRRRTDGTVDEILGGEEETVCSVGGVVTGLQRK
                     WTKRGDLMAVFQLEDLRGAIEVMVFPKTMTEHGHKLEDDAIVIVRGRVDRKEDTARVM
                     AMELERFEPVVEAPSVTIDLTRSAVNDDLLRRLKGVLADHPGESEVVLQLSDRQRVRL
                     ADDLWVDASNGLVAELRVLLGSNAVR"
BASE COUNT          459 a         1022 c         1132 g          554 t
ORIGIN      
        1 cggaccgcgt tcgaccccag gagtacccgc aactccgcca ccaggccgtt gctggcatcg
       61 acccagaggt cgtcggccag gcggacccgc tgacgatcgc tcagctgcag gacgacctcg
      121 gattcgccgg gatggtcggc caggacgccc ttcaaccgac ggaggaggtc gtcgttgacg
      181 gccgagcggg tcaggtcgat ggtgaccgac ggtgcctcga cgacgggctc gaagcgctcc
      241 agctccatgg ccatgacccg cgccgtgtcc tccttgcggt cgacccgacc ccggacgatg
      301 acgatggcgt cgtcctccaa cttgtggccg tgctcggtca tcgtcttggg gaagaccatg
      361 acctcgatgg caccgcggag gtcctccagc tggaacacgg ccatcaggtc gccccgcttg
      421 gtccacttcc gctggagccc ggtcacgacg ccgccgaccg agcagaccgt ctcctcctcg
      481 ccgccgagga tctcgtccac ggtgccgtcg gtgcgccgac gcagggagga ctcgacgccc
      541 atcaacgggt ggtcgctgat gtagaggccc agcatctcct tctcgaacgc cagcttgagc
      601 ttcttgtcca gctcgccttc gtcgatgcgg gggcggtcgt cgaacaccgg gccgtcgtcg
      661 atcgagtcga agaggctcat cgcccccatg tcgtgctgct tgcgcttggc cacggtcagg
      721 tcgatgatcc gctcgtaggc ggccagcagg cccttgcggg tgtgacccag caggtcgaag
      781 gcgccggact tgatcaaggc ctccacggtc cgcttgttca gggccgcggt gtccactcgc
      841 tcgcagaagt cgtagaagtc gacgaagggc ccgccggcct cgcgctgggc gaggatcagg
      901 tcggtcaccc cctcgcccac gttgcgcacg gcggacagcc cgaacgggat ggaccccagg
      961 gtcccggccg tcacgtcctc gggcggcacc gcggtgaact tggacaccga caggttgatg
     1021 tcgggcacgc ggacctcgat gcccatcgtc cggcactcgg ccaggtagat ggcggccttg
     1081 tcctggctgt ccttgaccga ggtcagcagc gcggccaggt actcgaccgg gtagtgggcc
     1141 ttcagccagg ccgtctggta ggcgatgagg ccgtagccgt aggcgtggct cttgttgaac
     1201 gcgtagtcgg cgaacggctc gatgatgtcg aagagctggt cgccgaggtc ccggccgtac
     1261 cccgtggtct cgcagccggc gacgaacttg tcgcgctcgg cccggatcat ctcccggatc
     1321 ttcttgccgc aggccttgcg caggttgtcg gcctcggcca gggagtaccc cgccaccttc
     1381 tgggccaccc gcatgaccga ctcctggtag atcatcaggc cgtaggtgtc acccagggtc
     1441 tcctccaggt cggggtggag gtaggtgacc ggcttccgcc cgttcttgcg gtcggcgtag
     1501 tcgttgtgca tgttcgcccc catgggcccg gggcggtaca gggcgacgag ggcggcgatg
     1561 tcgtcgaagc tgtccggggc cagggagcgc atgagcgccc gcatcgggcc gccctccagc
     1621 tggaacaccc cgatcgactc cccccggcac aacatggcga gggtcttcgg gtcgtcgagc
     1681 gggacgttgt cgatgtcgac gtcctcgccc cgcacgtccc ggatcagctc gagggtgtcc
     1741 tcgatgacgt ccaggttgcg caggccgagg aagtccatct tgagcaggcc cagcgactcg
     1801 atgccgttca tctcgtactg ggtgacgatc ggggcgtcct cgggggcctg gcccgactcg
     1861 ggcttgcgct ggacgggggc gtactcggtg acgggctggt cggtgatcac cacggcggcg
     1921 gcgtggatgc tgtcctggcg gcggaggttc tcgaggccgg tggccacgtc gatgacggcc
     1981 cgggcgtcgc tgtcggactc gtagacggtc cggagctcgc cggccatctt gtacccctct
     2041 tcgaacttct cggtcttctc caggcaggcg tgcagtggcg tgtcccggcc catgatcagc
     2101 ggtggcatca gcttggccac cttgtcgccc agggcgtagg ggtacccgag cacccgggcg
     2161 gcgtcccgca cggcggcccg ggccttgatc gtcgagaagg tgacgatctg ggcgacccgg
     2221 tcccggccgt agcgctcggc cgcgtagcgg atcatctcgt cccggaagcg ggagtcgaag
     2281 tccatgtcga tgtcgggcat ggagatgcgg gacgggttca ggaagcgctc gaacagcagg
     2341 tcgtacttga tggggtcgag gtcggtgatg cggaggcagt aggccacgca gcacccggcg
     2401 gcgctgcccc ggcccggccc gacccggatg cccttgtccc gggcgtgctt gatgaggtcc
     2461 cagacgatga ggaagtagga cgagaagccc atgtcctcga tgacctggag ctcgaaggcg
     2521 acgcgctcca ggacggcgtc ggggagctgc tggccccacc gggccttggc cccctccagg
     2581 gtcaggtgct tgaggtaggc cgagtcctcg gtgaagccgt cgggcagagg gaaccggggc
     2641 aggagcggct tgccgaactc gatctcgacg ttgcagcgtt cggcgatgag gagcgtgttg
     2701 tcacaggcgc ccgggtagtc ggtctcgggg aacaggcggc gcatctcgtc ggccgacttg
     2761 aggtagtgct cctccccgtc gaacttgaag cgggtcggat cgctcatcat cgagttggtc
     2821 tggacacaga gcagggcgtc gtgggccttg gcgtcctccc gggcgacgta gtggctgtcg
     2881 ttggtggcca ggaggggggc gccgagcttg cgggagatgg ccagcaggcc gggcatggtc
     2941 tggcgttgcc tgtcgatccc gtggtcctgg atctcgacga acaggttgtc cttgccgaag
     3001 atgtcctgga ggcgaccggc ccgggtcagg gcttcggctt cgtcacccgt catcagggcc
     3061 tgcaggacgt ggccaccgag gcaggccgtg gtggcgatga ggccttcgct gtgctcgtcg
     3121 aggagctccc agtcgatgcg gggcttgtag aagtacccct cgaggaa
//
LOCUS       DASTFV010000647         2588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2447476, whole genome shotgun sequence.
ACCESSION   DASTFV010000647 DASTFV010000000
VERSION     DASTFV010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2447476"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFP02_11850"
     CDS             complement(<1..184)
                     /locus_tag="VFP02_11850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HET9543774.1"
                     /translation="MIIVSGTLTIDPADVEAAMALGATMAEASLAEEGCRAYGVWQDP
                     ADPSRARVFEEWASQEA"
     gene            complement(293..541)
                     /locus_tag="VFP02_11855"
     CDS             complement(293..541)
                     /locus_tag="VFP02_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543775.1"
                     /translation="MARSGETFAKRQREKARQEKQAAKRARRLDKEEDGLETEEAPDL
                     DALMEEFRIISEKHAAGELESEEFEERRDEILMQLGVE"
     gene            complement(543..725)
                     /locus_tag="VFP02_11860"
     CDS             complement(543..725)
                     /locus_tag="VFP02_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HET9543776.1"
                     /translation="MKFFDTRRGFGFIAVPGRDDLFVHHTNVTGSGGVLDEGEQVSFE
                     IAQGRKGDEARNVRVV"
     gene            complement(918..1910)
                     /locus_tag="VFP02_11865"
                     /pseudo
     CDS             complement(918..1910)
                     /locus_tag="VFP02_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(2112..>2588)
                     /locus_tag="VFP02_11870"
     CDS             complement(2112..>2588)
                     /locus_tag="VFP02_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543777.1"
                     /translation="GLALIAAFGISTFVVAVGLIGDWSRDALVFAVVVGGVTAIAPVI
                     LVRFGLDLGPIRRLPAVSAAEMKAAAGTIGTKLLDRERRFVEAKGLRRVFALGGALWD
                     LRKDVEGLKEGGLAPAAALVETLVPTRLIRVGISALVAPVALAVGMMSMLYAVITT"
BASE COUNT          361 a          881 c          968 g          378 t
ORIGIN      
        1 gcgcctcctg ggaggcccac tcctcgaaca cccgggcccg ggacgggtcg gcggggtcct
       61 gccagacgcc gtaggcgcgg cacccctcct cggccaggct ggcctcggcc atggtggccc
      121 cgagggccat ggcggcctcg acgtcggcgg ggtcgatggt gagcgttccg gaaacgatga
      181 tcatggcgac agcttgcccc atcgccggcg caggcggcgc cgccggccga aaacggacaa
      241 ggcccgctcc gtgcgtgcac gaagcgggcc tcgggccatt acggccgtga ggtcattcga
      301 cgccgagctg catcaggatc tcgtcccgac gctcctcgaa ctcctcgctc tcgagctcgc
      361 cggcggcgtg cttctcgctg atgatgcgga actcctccat cagtgcatcg aggtcgggag
      421 cctcttcggt ctcgaggccg tcctcttcct tgtccagtcg ccgggcgcgc ttggccgcct
      481 gcttctcctg gcgggccttc tcgcgctgcc gcttggcgaa cgtctctcca cttcgtgcca
      541 tgtcacacca ccctcacgtt gcgggcctca tcaccttttc ggccctgggc gatctcgaac
      601 gacacctgct cgccctcgtc gaggacaccg ccggacccgg tgacgttcgt gtggtgcacg
      661 aacaggtcgt cgcggccggg cacggcgatg aaaccgaagc cacggcgggt gtcgaagaac
      721 ttcaccgtac cggttggggc accgtttttc cgaccaccat gggggcgctc ggagcgggcc
      781 cgcgggacct ccgccgagag ggctccgggg gccgtgatct cgcccggcag ccccagcttg
      841 cgctgcatct tgcggttgtc ggacagctcc tcgctgcgca ccagggagac cacgacgccg
      901 gtggcgccgg cccgggcggt gcggccggaa cggtgcaggt aggtcttctc gtcctcgggc
      961 ggatcgaagt gcagcacgca ggccacaccg tcgacgtgga tgccccgggc ggccacgtcg
     1021 gtggcgatca gggcgtcgac ccggcccttc atgaacgact ccagggcccg gtcccgctgg
     1081 ccctgggaac ggccaccgtg gatggctgcg gctcgcacct tgctcttgcc cagctgctgg
     1141 gccaggcggt cggcgccgcg gcgcgtccgg cagaagacga tggtcgggcc ggcgtccgtg
     1201 atgacgtcga cggcgtggtc cttgcggtcc atgtgggcga ccttccagaa caagtgccgg
     1261 gaggtggtga tgtcgggctc gacgtcaccc acctcgtggc gggtggggcg gtcctggtag
     1321 cggtcggtga cgaccttgac gtcaccgtcc agcgtggccg agaacagcac ggtctggcgg
     1381 tcgtcgggtg tggcgtcgag caggcgcttg acggcgggca ggaagcccat gtcggccatg
     1441 cggtcggcct cgtcgatgac cacgatctcg atgtcctgga gctcgacgtc gcggcactgg
     1501 atcaggtcct cgaggcggcc ggggcaggcc accaggacgt cgacgccctt gcgcagcgcg
     1561 ctgcgctggc cgccgtagcc gacgccgccg tagaccgaca cggcccggcg cttgcgaatg
     1621 gcgagcaggg gcgtgagctc cttgtggatc tgctcggcca gctcacgggt gggggacagg
     1681 accagcgccc gggggcggcg gggcgtgccc ttgccgaccc gggtggccag ggggatgccg
     1741 aaggccaggg tcttgcccga gccggtcggg gcccgaccgc agacgtcgcg gccggcgagg
     1801 aggtccggga gggtggccgc ctgaatcggg aacgggtcgg tgacgccctt ctgggagagg
     1861 acccggacga cgtcgtcggg cacgccgaga tcggcgaagg agggggacat gagggggtag
     1921 tgctcctgac tgcccgcttc gagcggaccg gcgcacgtcg cgccgcggat cgaaacggtg
     1981 gagaggcgat ccagccggaa cgggggttga tgggggatgt gccgtgccgg ggagcttcga
     2041 gtaagacgct tgcctccacc cggcggcagt ggcgcaggat agcggggggc ggggcggctt
     2101 cctcggggcc ctcaggtggt gatgacggcg tagagcatgc tcatcatccc gaccgccagc
     2161 gcgaccgggg cgaccagcgc gctgatcccg acccggatga ggcgggtggg gaccagggtc
     2221 tcgaccaggg cggccgcggg tgcgaggccg ccctccttca acccctccac gtccttgcgc
     2281 aggtcccaga gcgccccacc gagcgcgaag acccggcgca agcccttggc ctcgacgaac
     2341 cgccgctccc ggtcgaggag cttggtgccg atcgtgcccg ccgcggcttt catctcggcg
     2401 gcggacacgg ccggcagtcg gcggatgggc cccaggtcca gcccgaatcg gacgaggatc
     2461 acgggggcga tcgccgtcac cccgccgacg acgacggcga acacgagggc gtcacgggac
     2521 cagtcgccga tcaggccgac ggccacgacg aacgtgctga tgccgaaggc ggcgatcagg
     2581 gcgaggcc
//
LOCUS       DASTFV010000648         6641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2448006, whole genome shotgun sequence.
ACCESSION   DASTFV010000648 DASTFV010000000
VERSION     DASTFV010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2448006"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFP02_11875"
     CDS             <1..314
                     /locus_tag="VFP02_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543778.1"
                     /translation="RSRYMLGFLFSTPAYRRTLELHGWEDLGDRLRSMSKEGRWEDLE
                     ATVTDDVLDTVVAAGRYDELARVLGDRYADLADGLLLHAPTDPADDEPFAAAVEALQA
                     V"
     gene            515..946
                     /locus_tag="VFP02_11880"
     CDS             515..946
                     /locus_tag="VFP02_11880"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HET9543779.1"
                     /translation="MSTLDWDDYFDSLDYDPASALGCWEYADRAVDVPSLTALTDALN
                     DLGAQGWEVITTTMFPSITTNKMVVVAKRRLLSPPAPDDPAAGWRDDPTGRFDKRYWD
                     GRFWSAHVGTIHPKSIEKDPPTMLSPPPDIDDGHKDRLPSS"
     gene            956..2173
                     /locus_tag="VFP02_11885"
     CDS             956..2173
                     /locus_tag="VFP02_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9543780.1"
                     /translation="MTDTLDTAVDPETTEVEEALEAFLAANDPSALSPVEYRGARYDA
                     GLACVHFPEGFGGLGVRPKLQAVVERRLREAGAPPPADTLFFGLALAGPTVVTHGSDD
                     VRHRLLRPMFTGEEVWCQLFSEPGAGSDLAGLSTRAVQDGDEWVVNGQKVWNTIAHLA
                     DRGMLVTRTDPDQPKHKGLTYFAVDMHDPGVEVRPLRQITGEAEFNEVYLTDVRVPDA
                     DRIGDVGDGWRVSMTTLMNERTTIGGGGGTRPRGSGPIAEAMRIWAAKSDKDPVERDK
                     LMKLWIEAETLRLTTLRASAAAKAGNPGPEGSVLKLRFAEMNKAIYELCLDLLGPAAL
                     VDYDYTMRRSDIIGLEGPPGASRKMFLRSRANSIEGGTSEIMRNILGERMLGLPGEPR
                     TDKDQPWIDVPRN"
     gene            2234..3598
                     /locus_tag="VFP02_11890"
     CDS             2234..3598
                     /locus_tag="VFP02_11890"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HET9543781.1"
                     /translation="MHEPVLLRDVPTMADWWEAGGGIGLDRAEKLGVGGTLQELGLAG
                     LRGRGGAGFPTARKWRSIGQARPETGDRYVVVNAAEGEPGTFKDRALIRANPYQLIEG
                     AAIAAVTVGATKAYIGLKKTFRTEIDRLERALTEMEAEGLTAGVDFELVAGPDHYLLG
                     EETGLLQVIEGEEPLPRWAPPYQHGLFATGPQAGWSSAPKRDAEPTNQSNPTLVNNVE
                     TLSSVPHILALGPEWYRSMGTEESPGPIVCTIVGDVVTPSVVEVEMGTPLIELIETHA
                     GGLAEGRYVKAVLSGFANPALTGADLDAPISYEGLRDAGGGLGSAGFIVYDDRTNMVE
                     VARLASNYLYVESCGQCSPCKLGSGDITERLDRIARSGGDEGDIEAIGARLLTVTDAN
                     RCYLGTQEQLLVSSILRLFPADVVAALEGGGIEGRGLEVPKLIELDQYDPDQHRKRAD
                     WTYT"
     gene            3701..3880
                     /locus_tag="VFP02_11895"
     CDS             3701..3880
                     /locus_tag="VFP02_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543782.1"
                     /translation="MATVMTDDERRAFLLEGTRTGKVAWISPQGNVHVAPVWFVLDGT
                     TSSSPPTRTARRARR"
     gene            3877..4128
                     /locus_tag="VFP02_11900"
     CDS             3877..4128
                     /locus_tag="VFP02_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543783.1"
                     /translation="MTDGCRLSLVVDDQAPPFSYVKVDGTVSLSDDLDELVAVATRIG
                     GRYMGEDRAEEFGRRNGVPGELVVRLSPTKLTALAAISD"
     gene            complement(4138..5418)
                     /locus_tag="VFP02_11905"
     CDS             complement(4138..5418)
                     /locus_tag="VFP02_11905"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543784.1"
                     /translation="MRRRLYPVLLIALALSTAAACSDDGGDDSATEATAPPATFAPAS
                     DAEPADFSGRGPYAVGEIDLQLDPDHLVAVFYPVDRSTVTPDAEAYTYSGEDILDPTI
                     AAILPSALSGEVAPPDTWVGLPASGNGPFPTVLHSHGFSGNLRFGNQHNAAVASWGYV
                     VAAVDHPERGLASILDSFISGEADDAERPDEYLDSDQLLAGLDLLAETNDQADSPLAG
                     VIDTERVAAEGHSAGGSASGTAAYDDRVDLWIGQAPGTPLAPDTDLEAFITETDDGPD
                     VDTTALLEATTPPDVPSLIIAVEGDTIVTLDRVQQTYDWLDSPKRLAVIADSGHAVFV
                     DPCVPIRDEGGLSGFVEALGLDPADVPLVELGENGCLPDDTDPEPVWGLINHLTVAQL
                     DQVFAIDQDVATASLEPDYLDATFPDLLADIRTD"
     gene            5477..6280
                     /locus_tag="VFP02_11910"
     CDS             5477..6280
                     /locus_tag="VFP02_11910"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET9543785.1"
                     /translation="MAYVEVSDPRPHVRQIMLNRPERMNAMSFDTIIPLREALEAAGQ
                     DNDCWVVVLTGKGDGFCSGLDLEDAGVPPHADGLTLSRLAIRAMEYFGDIVPQLRKLP
                     QPVIGAINGPAIGGGMCLSMGTDIRIAGQSAYWRAAGINNGLGAVELGLSWTLSRAIG
                     SSRAFEICLSGRDVHAEEAERIGLVSRTVPDAELLDTTYKLAERICGFSTHGVSMTKK
                     VLWSALEVGSLEAALDLENRNQLLVRLTTQNLEEYIRSRREGRTPTYLD"
     gene            complement(6348..>6641)
                     /locus_tag="VFP02_11915"
     CDS             complement(6348..>6641)
                     /locus_tag="VFP02_11915"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HET9543786.1"
                     /translation="EERFAARGDAMNDVEGFVSFELLRPTAGEDRYFVYTRWETEAAF
                     PAWRESQRFAESHAGQTGPPPEGHGGAEKGSGPPVGMGADLLEFEVALAVTKA"
BASE COUNT          992 a         2285 c         2399 g          965 t
ORIGIN      
        1 cccgcagccg ctacatgctc ggcttcctct tctccacgcc cgcctaccgt cggaccctcg
       61 agctgcacgg ctgggaggac ctcggcgatc ggctccgctc gatgtccaag gagggtcgtt
      121 gggaggacct cgaggccacg gtcaccgacg acgtcctcga cacggtggtc gcggcgggac
      181 gctacgacga gctggcccgg gtcctgggtg accgctacgc cgacctggcc gacggtctcc
      241 tgctccacgc cccgaccgat cccgccgacg acgagccttt cgccgccgcc gtcgaggcgc
      301 tccaggccgt ctgactccag cccgctgtcc cctcaatccg acggttcgcc cacccgatcc
      361 agggcgaaca tttcttcgca ctttcccggg acgtgtggta acgctggtcg tggtgcggga
      421 cgttgcgaac aggctctatg tctgtcccac gtgcgggaca aggcccacgt cgggcaacca
      481 ttggccgaac ggcgtctccg gcgcccattc ggccatgtcg accttggact gggacgacta
      541 cttcgactcg ctggactacg acccggcgtc ggctctcggc tgctgggagt acgccgacag
      601 agccgtagat gtcccgtcgt tgacggccct cactgatgcg ctcaacgacc tcggagcgca
      661 ggggtgggag gtcatcacga ccaccatgtt cccgagcatc acgacgaaca agatggtcgt
      721 cgtggccaag cggcgacttc tgtcgccgcc ggcaccggat gaccccgccg cgggatggcg
      781 ggacgacccg acaggccggt tcgacaagcg ctattgggac ggtcgcttct ggtcggccca
      841 cgtcggcacc atccatccga agtcgatcga gaaggacccg cccacgatgc tgtcgccgcc
      901 cccggacatc gacgacggac acaaggaccg actgccgtcg tcgtagggtc ggaccatgac
      961 cgacacgctc gacaccgccg tcgatccgga gaccaccgag gtcgaggagg cgctcgaggc
     1021 cttcctcgcc gcaaacgacc catccgccct gagccccgtc gagtaccggg gggcccgcta
     1081 cgacgccggc ctggcgtgcg tccacttccc cgaaggcttc ggtggcctcg gtgtccgccc
     1141 caagctgcag gccgtcgtgg agcgtcgctt gcgggaggcc ggggcgccac caccggcgga
     1201 caccctcttc ttcggcctgg ccctggccgg gcccaccgtc gtcacccacg gctccgacga
     1261 cgtgcgccac cgcctgctcc gcccgatgtt caccggtgag gaggtctggt gccagctctt
     1321 cagtgagccc ggtgcgggct cggacctggc cggcctgtcg acccgggccg tccaggacgg
     1381 ggacgagtgg gtggtcaacg gccagaaggt ctggaacacg atcgcccacc tcgccgaccg
     1441 cgggatgctg gtcacccgta ccgatccgga ccagcccaag cacaagggcc tgacctactt
     1501 cgcggtggac atgcacgacc ccggcgtcga ggtccgcccg ctgcgccaga tcaccggcga
     1561 ggccgagttc aacgaggtgt acctgaccga cgtgcgcgtc cccgacgccg accgcatcgg
     1621 cgacgtgggc gacggctggc gggtgtcgat gaccacgctg atgaacgagc gcacgaccat
     1681 cggtggtggc gggggcaccc gcccccgggg gagcgggccc atcgccgagg ccatgcgcat
     1741 ctgggcggcc aagtccgaca aggacccggt cgaacgggac aagctcatga agctgtggat
     1801 cgaggccgag accctgcggc tcacgaccct gcgggccagc gccgcggcca aagccggcaa
     1861 ccccgggccc gagggctcgg tcctcaagct ccggttcgcc gagatgaaca aggccatcta
     1921 cgagctctgc ctcgatctcc tcgggccggc cgccctcgtc gactacgact acacgatgcg
     1981 ccgctccgac atcatcggcc tggagggccc accgggtgcg tcccgcaaga tgttcctgcg
     2041 ctcccgggcc aactccatcg agggcgggac ctcggagatc atgcgcaaca tcctgggcga
     2101 gcggatgctc ggcctccccg gcgagccgcg gacggacaag gaccagccct ggatcgacgt
     2161 cccccggaac taggtcccac cgtcgcgcgt ggtcgatcgg gtgaaggctg ccctgatccg
     2221 gtaggcaggg tccgtgcacg agccggttct gttgcgtgac gtgcccacga tggccgactg
     2281 gtgggaggcc ggtgggggca tcggcctcga ccgagccgag aagctggggg tcgggggcac
     2341 gctccaggag ctgggcctcg ccggcctgcg ggggcgaggg ggggccgggt tccccacggc
     2401 ccgcaagtgg cgctcgatcg gccaggcgcg gcccgagacc ggcgaccggt acgtcgtggt
     2461 caacgccgcc gagggcgagc ccggcacctt caaggaccgg gcgctcatcc gggccaaccc
     2521 ctaccagctc atcgaggggg cggccatcgc cgccgtgacc gtcggggcga ccaaggccta
     2581 catcgggctg aagaagacgt tccgcaccga gatcgaccgc ctcgagcggg ccctcaccga
     2641 gatggaggcc gaagggttga ccgcgggtgt cgacttcgag ctggtcgccg ggcccgacca
     2701 ctacctgttg ggcgaggaga ccggcctgct ccaggtcatc gagggcgagg agccgctgcc
     2761 ccgctgggcc ccgccgtacc agcacggcct cttcgccacc ggcccgcaag cggggtggtc
     2821 gtccgccccc aagcgggacg ccgagccgac gaaccagtcc aaccccacgc tggtcaacaa
     2881 cgtggagacc ttgtccagcg tcccgcacat cctcgccctt ggcccggagt ggtaccggtc
     2941 gatggggacc gaggagtcgc ccggcccgat cgtctgcacg atcgtcggcg acgtcgtgac
     3001 gccgtccgtg gtcgaagtcg agatgggcac gccgctcatc gagctcatcg agacccacgc
     3061 cgggggcttg gccgaggggc gctacgtgaa ggccgtcctg tccggcttcg ccaacccggc
     3121 gctgaccggg gccgacctgg acgccccgat cagctacgag gggctgcgcg acgccggtgg
     3181 tggactgggc tcggccgggt tcatcgtcta cgacgaccgg accaacatgg tcgaggtggc
     3241 ccggctggcc agcaactacc tctacgtcga gagctgcggg cagtgctcac cctgcaagct
     3301 cgggtcgggg gacatcaccg agcgcctcga ccggatcgcc cggtccgggg gtgacgaggg
     3361 ggacatcgag gccatcgggg cccgcctgct caccgtgacc gacgccaacc gctgctacct
     3421 cggcacccag gagcagttgc tggtctccag catcctgcgc ctcttcccgg cggacgtggt
     3481 cgcggccctc gagggtgggg ggatcgaggg gcggggtctc gaggttccca agctgatcga
     3541 gctggaccag tacgaccccg accaacaccg caagcgcgcc gactggacgt acacatagct
     3601 ctgcctccgc tccgccggct tcgccgatgc ggctggccgg acgagctgct gcgctccgct
     3661 ccgcagcgtc ccgacccgac accgactgag gagacctgag atggcgacgg tgatgaccga
     3721 tgacgagcgg cgggccttcc tgctggaggg gacccggacc gggaaggtgg cgtggatctc
     3781 gccccagggc aacgtccacg tggcccccgt ctggttcgtg ctcgacggga cgacgtcctc
     3841 ttcaccaccg acgaggacag cgcgaagggc aaggcgttga ccgacggctg ccgcctgtcg
     3901 ttggtggtcg acgaccaggc cccgccgttc tcctacgtga aggtcgatgg cacggtgtcg
     3961 ctcagcgacg acctcgacga gctggtcgcg gtggccaccc gcatcggtgg ccgctacatg
     4021 ggtgaggacc gggccgagga gttcgggcgc cgcaacggcg tccccggcga gctcgtggtc
     4081 cgcctctcac ctacgaagct gaccgccctg gccgccatca gcgactgacc accagggcta
     4141 atcggttcgg atgtcggcca ggaggtcggg gaaggtggcg tcgaggtagt ccggctccag
     4201 cgaggcggtg gccacgtcct ggtcgatggc gaagacctgg tcgagctggg ccacggtgag
     4261 gtggttgatg aggccccaca ccggctcggg gtcggtgtcg tcgggcaggc agccgttctc
     4321 acccagctcg acgagcggga cgtcggccgg gtcgaggccg agggcttcga cgaagccgct
     4381 gaggcccccc tcgtcacgga tcgggacgca cgggtcgacg aacacggcgt ggccactgtc
     4441 ggcgatgacg gcgaggcgct tgggcgagtc gagccagtcg taggtctgtt ggacccggtc
     4501 gagggtgacg atcgtgtccc cctcgacggc gatgatcagg gagggaacgt cgggtggcgt
     4561 ggtggcctcg agcagcgccg tcgtgtcgac gtcgggaccg tcgtcggtct cggtgatgaa
     4621 ggcctcgaga tcggtgtccg gcgcgagggg ggtgccgggg gcctggccga tccagaggtc
     4681 gacccggtcg tcgtacgccg ccgtgcccga ggcgctgcca ccggcgctgt ggccctcggc
     4741 cgcgacccgc tcggtgtcga tgacccccgc gaggggggag tcggcctggt cgttggtctc
     4801 ggccaacagg tcgaggccgg cgaggagctg gtcgctgtcg aggtactcgt cgggccgttc
     4861 ggcgtcgtcg gcctcgccgg agatgaagct gtcgagtatc gacgccagac ctcgctcggg
     4921 gtgatccacc gcggcgacga cgtagcccca cgaggcgacg gcggcgttgt gctggttgcc
     4981 gaagcggagg ttgccggaga agccgtgact gtggagcacg gtcgggaagg gtccgttgcc
     5041 gctggcgggc aggccgaccc aggtgtccgg cggagcgacc tcaccggaga gggcgctcgg
     5101 gaggatggcg gcgatggtcg ggtcgaggat gtcctcgccg gagtaggtgt aggcctcggc
     5161 gtcgggggtc accgtgctcc ggtcgacggg gtagaagacg gcgacgaggt gatcggggtc
     5221 gagttgcagg tcgatctcgc ccacggcgta agggcctcgg cccgagaagt cggcgggctc
     5281 ggcgtcggag gcgggggcga aggtcgccgg cggcgccgtg gcctccgtgg ccgagtcgtc
     5341 gcccccgtcg tcggagcagg ctgcggcggt gctcagggca agggcgatga ggaggacggg
     5401 gtacagcctg cggcgcatcg tcgtttccta gcaccggccg gactcggccc tgtccgcggg
     5461 ctgccaagat caggcgatgg cctacgtcga ggtgagcgat ccccgtcccc acgtccggca
     5521 gatcatgctg aaccggccgg agcggatgaa cgccatgtcg ttcgacacga tcatcccgct
     5581 gcgggaggcg ctggaggcgg ccgggcagga caacgactgc tgggtcgtgg tcctgacggg
     5641 gaagggcgac gggttctgct ccgggctgga cctggaggac gccggggtcc caccccacgc
     5701 cgacgggctg accctgtccc gcctggccat cagggccatg gagtacttcg gcgacatcgt
     5761 cccccagttg cgcaagctgc cccagccggt gatcggagcg atcaacggcc cggccatcgg
     5821 gggcggcatg tgcctgtcga tggggaccga catccgcatc gccggtcagt cggcctactg
     5881 gcgggcggcg gggatcaaca acggcctcgg tgccgtcgag ctgggcctct cgtggacgct
     5941 gtcccgggcc atcgggtcgt cccgggcgtt cgagatctgc ctgtcgggcc gggacgtgca
     6001 cgcggaggag gccgagcgca tcggcctggt gtcccgcacg gtgcccgacg ccgagctact
     6061 ggacacgacc tacaagctgg ccgaacgcat ctgcgggttc agcacccacg gggtgtcgat
     6121 gaccaagaag gtgctgtggt ccgccctcga ggtcggcagc ctggaggcgg cgctggacct
     6181 ggagaaccgg aaccagctgc tggtccgcct gaccacccag aacctggagg agtacatccg
     6241 ctcccgccgc gagggccgga cgccgaccta cctcgactga tcccgaactt gcgtcgggta
     6301 caccacagat ctgtggttca ttcgacgcat gttcgggcgg aggggggtca ggccttggtg
     6361 acggcgaggg cgacctcgaa ctccagcagg tcggccccca tgcccacggg tgggccggag
     6421 cccttctcgg ccccgccgtg gccctccggg ggcggtccgg tctggccggc gtgcgactcg
     6481 gcgaagcgct ggctctcccg ccacgccggg aaggcggcct cggtctccca gcgcgtgtag
     6541 acgaagtagc ggtcctcgcc ggcggtcggc cgcagcagct cgaaggacac gaagccctcg
     6601 acgtcgttca tggcgtcgcc ccgcgccgcg aagcgctcct c
//
LOCUS       DASTFV010000649         3026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2449711, whole genome shotgun sequence.
ACCESSION   DASTFV010000649 DASTFV010000000
VERSION     DASTFV010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2449711"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="VFP02_11920"
     CDS             complement(<1..1138)
                     /locus_tag="VFP02_11920"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9543787.1"
                     /translation="MTVSRRQFLIGLGAVGGAGAVLGGLAVIDRGSGDRRPFRAPAAG
                     DFTLQGRTNETSVLVLGAGVAGLACAYELEKAGYAVTVLEARGRTGGRNLTVRPGTEL
                     TDTRGTIQRADLGEGRWFNAGPARIAAHHTTIDYCRELGVAIEPFVNVNVDAYVVANG
                     EVRRRRSLDADLDGYVAELLTKAIPTGVLDDEITADEKDALYAYLQGVGAIAPVERGY
                     DESPGAGEQAGEIQDPDQLATVLAFDPGMRRYFERDWHLSTPMFHPVGGMDALPLALT
                     DALVGEIHTAAEVRSVGADGDGVSARLVDGSEIRADHGICTLPPWIAAALASLWDPVV
                     TQALQTPVRFATGKIGLEYDRRFWETGDRILGGITTTDREPREIW"
     gene            1182..1979
                     /locus_tag="VFP02_11925"
     CDS             1182..1979
                     /locus_tag="VFP02_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543788.1"
                     /translation="MHPIERLRYVARAHGASQAVMVSETAAALRSFGRDPQGLVTACR
                     RIVARQPSSGPLWWLAARTLTASDPIAEAQLAAAEIEDDRTPRAVADALPDEATVLVL
                     GWPELAAGGLPRRGDVEVLVCDVDGDGSSLVLRLLRADVDATDVPLSGLGAAAAEADV
                     VILETPAMGPAGFVAGQGSRAAACVARLSGAEVWLVAGVGRLLPERLFTALCDRTISA
                     DSPWDDELELVPLDLVDQVVGPTGPARPAEALRRTDCPIAPELLKPF"
     gene            2031..2972
                     /locus_tag="VFP02_11930"
     CDS             2031..2972
                     /locus_tag="VFP02_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HET9543789.1"
                     /translation="MSERTRDLPRRSCLSVPGSSEKMLGKGPTLPADMVFLDMEDSVA
                     PLEKEGARAKVVDAIKNQDWGEKILCVRVNAWDTQWTYGDIIEVVGNAGERLEEIMLP
                     KVQSAAEVVAMDLLLTQVEKNSGLPVGHIGIEAQIETARGLINVNEICAASPRLETVI
                     LGPVDFSASMEMPSLSGGLQIPEYPGDYFHYVFMKILMAGRANGLQVIDGPYVKVRDL
                     EGFRDFCTRTQTLGFDGKWALHPDQVTILNEMFSPTQAQFDRANDILDAYEEATTTGD
                     RKGAVMFGDEMIDEASRKVAIKLVSRGERAGLVRSPA"
BASE COUNT          421 a         1078 c         1071 g          456 t
ORIGIN      
        1 accagatctc cctgggctcg cgatccgtcg tggtgatgcc gccgaggatg cggtcgccgg
       61 tctcccagaa gcggcggtcg tactccaggc cgatcttgcc tgtggcgaag cggactggcg
      121 tctggagggc ctgggtcacc accgggtccc acagcgacgc cagcgctgcc gcgatccacg
      181 gcggcagggt gcagatgccg tggtcggccc ggatctcgct tccgtccacc aggcgggccg
      241 agacgccgtc accgtcggcg ccgacggacc ggacctcggc cgcggtgtgg atctcgccga
      301 cgagtgcgtc ggtcagggcg agggggaggg catccatgcc gccgacgggg tggaacatcg
      361 gggtcgagag gtgccagtcc cgctcgaagt agcgccgcat gccgggatcg aaggcgagga
      421 ccgtggccag ctgatcgggg tcctggatct cacccgcctg ctcgccggcg cccgggctct
      481 cgtcgtagcc ccgctcgacc ggggcgatgg cgccgacgcc ctgcaggtag gcgtagagcg
      541 cgtccttctc gtcggcggtg atctcgtcgt cgaggactcc ggtcgggatc gccttcgtca
      601 gcagctcggc gacgtagccg tcgagatcgg cgtcgaggct ccgccgccgg cggacctcgc
      661 cgttggcgac cacgtaagcg tcgacattga cgttcacgaa gggctcgatg gcgacgccga
      721 gctcccggca gtagtcgatg gtcgtgtggt gggcggcgat ccgtgccggt ccggcgttga
      781 accagcgccc ctcaccgagg tcggcccgct ggatcgtgcc ccgggtgtcg gtcagctcgg
      841 tgcccgggcg gaccgtgagg ttccggccgc cggtccggcc gcgggcctcg agcaccgtga
      901 ccgcatagcc cgccttctcc agctcgtagg cgcaggccag gccggcgacg cccgccccga
      961 ggacgaggac ggaggtctcg ttcgttcgcc cctgcagcgt gaagtcgccg gctgcagggg
     1021 cccggaaggg tcggcggtcg ccgctgccgc gatcgatgac ggccaaccca ccgagcacgg
     1081 caccggctcc acccacggcc ccgagcccga tgaggaactg ccggcggctg acggtcacgg
     1141 cccgatactt ccactctgac cgatggctac cgtcggggct gatgcacccc atcgagcgcc
     1201 tgcgctacgt ggcccgggcc catggcgcct cgcaggcggt gatggtgtcg gagacggcgg
     1261 ccgcgctgag gtcgttcggc cgggatccgc agggcctggt cacggcgtgc cggcgcatcg
     1321 tcgcccgcca acccagctcg ggcccgttgt ggtggctggc ggctcgcacc ctgaccgcct
     1381 cggaccccat cgccgaggcc cagctcgccg ccgcggagat cgaggacgac cgcacacccc
     1441 gagccgtggc cgacgccctt cccgacgagg ccacggtcct ggtcctgggc tggcccgagt
     1501 tggcggccgg cggcctgccc cgccggggcg atgtcgaagt gctcgtctgt gacgtcgacg
     1561 gcgacgggtc gtcgctggtc ctgcgcctgc tgcgcgcgga cgtcgacgcc accgacgtgc
     1621 ccctgagtgg cctgggtgct gccgccgccg aggccgatgt ggtgatcctg gagacgccgg
     1681 ccatggggcc ggccgggttc gtggctggcc aagggtcccg ggccgcggcc tgtgtggccc
     1741 ggctgtcggg ggcggaggtg tggctggtcg cgggcgtcgg ccggctcctg cccgagcgcc
     1801 tgttcaccgc gctctgtgac cgcacgatct ccgccgacag cccctgggac gacgaactgg
     1861 agctggtccc gctcgacctg gtcgaccagg tcgtcggccc gaccggcccg gcccgcccgg
     1921 ccgaggccct acgccgaacc gactgcccca tcgcgccgga gttgctcaag ccgttctgag
     1981 tcagctaccg gccttggccc cgcctggagc cgtcgtcgta ggctggtctc atgtccgaac
     2041 gcacccgtga cctgccgcgc cggtcctgcc tctccgtccc cggttccagc gagaagatgc
     2101 tggggaaggg cccgaccttg cccgccgaca tggtcttcct cgacatggag gactccgtcg
     2161 cgcctctcga gaaggagggc gcccgggcca aggtggtcga cgccatcaag aaccaggact
     2221 ggggcgagaa gatcctctgt gtccgggtca acgcctggga cacccagtgg acctacggcg
     2281 acatcatcga ggtggtcggc aacgccggcg agcgactcga ggagatcatg ctccccaagg
     2341 tgcagtcggc ggccgaggtc gtggccatgg acctgctcct gacccaggtc gagaagaact
     2401 cgggcctgcc cgtcggccac atcggcatcg aagcccagat cgagacggcg cggggcctga
     2461 tcaacgtgaa cgagatctgc gcggcgtcac cccgcctgga gacggtcatc ctcgggccgg
     2521 tcgacttctc ggcctccatg gagatgccgt ccctgtcggg tggcctccag atcccggagt
     2581 accccggcga ctacttccac tacgtcttca tgaagatcct catggcgggc cgggcgaacg
     2641 gcctgcaggt catcgacggg ccctacgtga aggtccgcga cctggagggc ttccgcgact
     2701 tctgcacccg cacccagacc ctgggcttcg acggcaagtg ggcgctgcac cccgaccagg
     2761 tcacgatcct gaacgagatg ttctcaccca cccaggccca gttcgaccgg gccaacgaca
     2821 tcctcgacgc ctatgaggag gccaccacga ccggcgatcg caagggcgcg gtgatgttcg
     2881 gcgacgagat gatcgacgag gcctcccgca aggtggccat caagctggtg agccggggcg
     2941 agcgcgccgg cctggtccgc agcccggcct gaccaccagg ttcttgcctg attctcaact
     3001 tcctttgcac aggatctcgc cgagga
//
LOCUS       DASTFV010000650         2799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2450697, whole genome shotgun sequence.
ACCESSION   DASTFV010000650 DASTFV010000000
VERSION     DASTFV010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2450697"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFP02_11935"
     CDS             complement(<1..193)
                     /locus_tag="VFP02_11935"
                     /inference="COORDINATES: protein motif:HMM:NF017530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070273 - phosphatidylinositol-4-phosphate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HET9543790.1"
                     /translation="MLLAGDFVLLALDADGTPARGNTNQSSVAVGVTGALIAELAIDG
                     HVELTDGRLHLTGTRPTHPL"
     gene            complement(251..949)
                     /locus_tag="VFP02_11940"
     CDS             complement(251..949)
                     /locus_tag="VFP02_11940"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET9543791.1"
                     /translation="MIDVRRAGDRFHTAIDWLDSWHSFSFSSHYDPANVGHGLLLVSN
                     DDRVAAGQGFGAHAHQDMEIVTWVLEGALAHQDSAGNSGVITPGVAQRMSAGTGIRHS
                     EMNASRDEPVHFLQMWVRPDTVGVEPGYEQVDISDRLVPGELVPVASGRGHEGAVHLH
                     QQDAVLWAVRLDEGQTVTIPAAPHVHVFVARGSARLSASIDLTEGDAVRLTDEREQEL
                     AAAAADTEVLVWEV"
     gene            complement(960..1454)
                     /locus_tag="VFP02_11945"
     CDS             complement(960..1454)
                     /locus_tag="VFP02_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9543792.1"
                     /translation="MDLDLDRLAARRQELKDQYLQEREDRPASSARGVHHVALLSADV
                     ETTIRFYQDLLEFPLTELFENRDYEGSTHFFFDIGNGNTIAFFDFPGLDLGDYAEVLG
                     GLHHLAISMPPDRWAHVKAKLDDAGVGYDHIDGSSIYFRDPDGARLELISDPLGEMYG
                     SPVT"
     gene            1651..2340
                     /locus_tag="VFP02_11950"
     CDS             1651..2340
                     /locus_tag="VFP02_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543793.1"
                     /translation="MTLTDEQRLPAAPALEAVLPGGGVRRGSVVGLEGPGSTSLLWSL
                     LAPPTGAGSWAVLVGLPAVGLLAAADAGVALERLAVVAPPGGSWATVLGALLDGFDLV
                     VAAPDRKVRPADARRLTARARERGTVVVLAGGGAVSAWPEGADLRLALTTPDWEGLGR
                     GHGHLQRRRVHLEVSGRREAARPRAADLWLPAEAHPVAAVTPDLRVLAVDSVAMTTEA
                     TAGTVRPVRVA"
     gene            2337..>2799
                     /locus_tag="VFP02_11955"
     CDS             2337..>2799
                     /locus_tag="VFP02_11955"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543794.1"
                     /translation="MTSTRTMVVWCPDWALVAAGVDLSVPAVVLHANRVTATTPAARA
                     EGVRVGLRRREAQSRCPEVELVDHDPARDARAFEAVAGAVEAVTPRVELTRPGVCSFP
                     TRGPSRYFGGDEALARRVAGLVADAIAGPSRVGVADGPFAALLAARRGLVVP"
BASE COUNT          327 a          975 c         1092 g          405 t
ORIGIN      
        1 gcagcgggtg cgtcggccgg gtgccggtga ggtggaggcg gccgtcggtc agctcgacgt
       61 ggccgtcgat cgccagctcg gcgatcagcg ccccggtcac gcccacggcg acagatgact
      121 ggttggtgtt cccccgagcc ggcgtgccgt cggcatcgag ggccaggagg acgaagtctc
      181 cagccagcag catccaccgg gtctacccgc ctgtggctcc gaccaaaacc gtcggcggcc
      241 gagccgggtg tcagacctcc cagacgagca cctcggtgtc ggcggcggcg gcggcgagct
      301 cctgttcgcg ctcgtcggtg aggcggacgg cgtcgccctc ggtcaggtcg atcgacgccg
      361 acagccgggc cgagccccgg gccacgaaga cgtggacgtg cggggcggcc gggatggtga
      421 cggtctgacc ctcgtccagg cggacggccc agagcacggc gtcctgctgg tggaggtgca
      481 cggcgccctc gtggcctcgg ccggacgcca ccggcaccag ctcgccgggg accaggcggt
      541 cgctgatgtc gacctgctcg tagccgggct cgacgccgac cgtgtcgggc cgcacccaca
      601 tctgcaggaa gtggacgggc tcgtcccggc tggcgttcat ctccgagtgg cggatgccgg
      661 tgccggccga catccgctgc gcgacccccg gggtgatgac cccggagttc ccggccgagt
      721 cctggtgggc cagtgccccc tccagcaccc aggtcacgat ctccatgtcc tggtgggcgt
      781 gggcgccgaa gccctggccg gcggccaccc ggtcgtcgtt gctgaccagg aggaggccgt
      841 ggcccacgtt ggccgggtcg tagtggctgc tgaagctgaa gctgtgccac gagtccagcc
      901 agtcgatggc ggtgtggaag cggtcgccgg cgcgccggac gtcgatcacg gttctctcct
      961 cacgtgacgg gcgagccgta catctcaccc agggggtcgc tgatgagctc gaggcgggcc
     1021 ccgtcggggt cccggaagta gatggagctg ccgtcgatgt ggtcgtagcc gacgccggcg
     1081 tcgtccagct tggccttcac gtgggcccac cggtccgggg gcatggagat ggccaggtgg
     1141 tggaggccgc cgagcacctc ggcgtagtca cccaggtcga ggccggggaa gtcgaagaag
     1201 gcgatggtgt tcccgttgcc gatgtcgaag aagaagtggg tcgagccctc gtagtcccgg
     1261 ttctcgaaca gctcggtcag ggggaactcg aggaggtcct ggtagaagcg gatggtcgtc
     1321 tccacgtcgg cggagagcag ggcgacgtgg tggaccccgc gggcgctgga ggccggacgg
     1381 tcctcacgct cctgcaggta ctggtccttc agctcctggc ggcgggccgc gaggcggtcg
     1441 agatcgaggt ccatgccccc actgaacacc agtcgggggt cgatgttccc cgccgcttgc
     1501 cagtgccgaa cagatgttcg atactggtac gtccaaacag gagaaggctg gggagccgag
     1561 atgatgactg acgctgcgga gcggagcgca gccgctcgaa gccggttcgt cggatccggc
     1621 gtagccgttt tgcaggagcg ggtcaggccc gtgaccctga ccgacgagca gcgcctgccc
     1681 gcggccccgg ccctggaggc ggtcctgccc gggggtggcg tgcgccgggg ctcggtggtg
     1741 ggcctcgagg ggccgggctc gacgtcgttg ctgtggtccc tgctggctcc cccgaccggg
     1801 gccggttcgt gggccgtgct ggtgggcctg cccgccgtgg gcctgctggc tgcggccgac
     1861 gccggggtgg cgctggagcg cctggccgtg gtcgccccgc ccggcggttc gtgggccacc
     1921 gtgctggggg ccctgctcga cggcttcgac ctggtcgtgg ccgcccccga tcgcaaggtc
     1981 cgaccggctg acgcccgccg cctcaccgcc cgggcccggg agcgggggac ggtcgtggtc
     2041 ctggcgggag gcggggccgt ctcggcctgg cccgaaggag ccgacctgcg cctggccctc
     2101 accaccccgg actgggaggg gctgggccgg ggccatggcc acctgcagcg ccgccgggtc
     2161 cacctcgagg tcagcggtcg ccgcgaggcg gcccgccccc gcgccgccga cctctggctc
     2221 cccgccgagg cccaccccgt cgccgcggtc acccccgacc tccgcgttct ggcggttgat
     2281 tcggtcgcca tgacgaccga agcaaccgcc ggaacggttc gtccggtgcg ggtggcgtga
     2341 cgtcgacgcg gacgatggtg gtgtggtgcc cggactgggc gctggtggcg gcgggggtgg
     2401 acctgtccgt gcccgccgtg gtcctgcacg ccaatcgggt gacggccacc acccccgcgg
     2461 cccgggccga gggggtccgg gtgggactgc gccggcggga ggctcagtcc cgctgccccg
     2521 aggtggagtt ggtggaccac gaccccgccc gggacgccag ggccttcgag gccgtggccg
     2581 gggcggtcga ggccgtcacc ccccgggtgg agctgactcg ccccggggtc tgctcgttcc
     2641 ccacccgggg accctcgcgc tacttcgggg gcgacgaggc cctggcccgc cgggtggccg
     2701 gtctggtcgc cgacgccatc gccgggccga gccgggtggg ggtggccgac ggccccttcg
     2761 ccgccctcct cgccgcccgg cgcggcctgg tggtcccgc
//
LOCUS       DASTFV010000651         2521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2456864, whole genome shotgun sequence.
ACCESSION   DASTFV010000651 DASTFV010000000
VERSION     DASTFV010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2456864"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VFP02_11960"
     CDS             <1..604
                     /locus_tag="VFP02_11960"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9543795.1"
                     /translation="NGWAVANTTLMNERASLGGGGGSSATSLALPGSVAGHLERRAGD
                     FVAKGGRGGTATILAGRSTKLLQNLVAELGKNDDPLIRQDLMRLYSMDQISGWGVQRA
                     KAGQASPALPNLAKLMMSHMTRLSRDVGLRIVGPAGTVMGPDTPGAGMIQELALFSPA
                     PSIYGGTDEVQRNIIGERVLGLPKEPGPDKATPFSELPTG"
     gene            717..917
                     /locus_tag="VFP02_11965"
     CDS             717..917
                     /locus_tag="VFP02_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HET9543796.1"
                     /translation="MATGTVKFFNAEKGYGFISREGADDVFVHYSNIQGSGYRSLDEG
                     QTVEFDVAPGRKGEEAQNVRVV"
     gene            complement(1079..2263)
                     /locus_tag="VFP02_11970"
     CDS             complement(1079..2263)
                     /locus_tag="VFP02_11970"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HET9543797.1"
                     /translation="MTPPRSNGDRLGRIQPEPRQEESVKPLELFFDLVFVLGFTQCTA
                     LMAAQPTWPGIGRGMLALAVLWWAWVGYAWLTSVIDPEEGAIRLAMFAAMAGLLIVAL
                     CVPEAFGDRAVPFAVAYAVVRGGHILLYVLASRGDPAMRHSVAGLALSSGIAVSLLFG
                     AAFLDSGWQEALWVVAIVLDFGEPAVMGMDGWTLVPAHFAERHNLVIILALGESIVAL
                     GVASQSDLTAGVIVTAVLGIGLAAALWWTYFDVVALVTERRLTQAAEGRERNTLARDS
                     YTYLHYPMVAGIILVALGLERTLAHLDESLDHVAGFALLGGTAIYLLGHVAMRLRNAH
                     TLNVQRLVLAVVLLALVPAVTAVDAIVTLAVVNVALWVMIAYETSRYDDRRYRLRHGL
                     EP"
     gene            complement(2286..2420)
                     /locus_tag="VFP02_11975"
     CDS             complement(2286..2420)
                     /locus_tag="VFP02_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543798.1"
                     /translation="MSTVVPAHVNGVPLEELLSLAIPLSTCAAVGMRVAWRQLRASRR
                     "
BASE COUNT          431 a          851 c          893 g          346 t
ORIGIN      
        1 caacggctgg gcggtggcca acaccacgct catgaacgag cgggccagcc tcggcggggg
       61 cggcggctcg tccgccacct cgctggcact gccgggcagc gtcgccgggc acctcgagcg
      121 tcgggcgggt gacttcgtgg ccaagggtgg ccgcggtggc accgccacga tcctggccgg
      181 gcgcagcacc aagctgctgc agaacctcgt ggccgagctg ggcaagaacg acgacccgct
      241 gatccgccag gacctgatgc gcctctactc catggaccag atcagcggct ggggtgtgca
      301 gcgggccaag gcgggccagg cctcaccggc cctgcccaac ctggccaagc tgatgatgag
      361 ccacatgacc cggctctccc gcgacgtcgg cctgcgcatc gtcggaccgg cgggaacggt
      421 gatgggcccg gatacgccgg gcgccggcat gatccaggag ctggccctgt tcagcccggc
      481 cccgtcgatc tacggcggca ccgacgaggt gcagcgcaac atcatcgggg agcgggtgct
      541 gggcctgccc aaggagcccg gtccggacaa ggccaccccc ttcagcgagc tgcccaccgg
      601 ctagccagaa ctgacccatg agtcggtggg gttggtgttc ggcggcagcc tcgtaccctg
      661 accctctgac gagggggtcg ttcttggcca ccttgctgat gagagagaga acagcgttgg
      721 ctacaggaac cgtcaagttt ttcaatgccg agaagggcta tggcttcatc tcgcgtgagg
      781 gtgcggacga cgtgttcgtc cactactcca acatccaggg ctcgggttac cgatccctcg
      841 acgagggtca gaccgtcgag ttcgacgtcg cccctggtcg gaagggtgaa gaggcccaga
      901 acgtccgggt cgtctgatca ctagcgccta tcggcgccgc ccctgacggg gctgccgatc
      961 ggcaatcagt cagatccaag cgtttgactc tgaaaatcag cgacccgtcg acggcaacgt
     1021 cggcgggttg ctgtggtttc gggctaccgg gcgccgtttc gcataggggt cgaatgcgtc
     1081 agggctccag gccgtggcgg aggcggtagc ggcgatcgtc gtagcgcgac gtctcgtagg
     1141 cgatcatcac ccagagggcg acattgacca ccgcgagggt gacgatcgcg tccacggcgg
     1201 tcaccgccgg caccagggcc aggagcacga ccgcgagcac gaggcgctgg acgttgagcg
     1261 tgtgggcgtt gcggagccgc atggcgacgt ggcccagcag gtaaatggcg gtcccgccca
     1321 ggagggcgaa cccggcgacg tggtcgaggg actcgtcgag gtgggccagg gtccgctcca
     1381 gcccgagggc gacgaggatg atccccgcga ccatcgggta gtgcagatac gtgtaggagt
     1441 cccgggccaa ggtgttgcgc tcccggcctt cggccgcttg ggtcaaccga cgctcggtga
     1501 ccagggccac gacgtcgaag taggtccacc agagggcggc ggccagcccg atcccgagca
     1561 ctgcagtgac gatgacgccg gcggtgaggt cggactggga agcgacaccg agggccacga
     1621 tggactcacc gagggcgagg atgatcacca ggttgtggcg ctcggcgaag tgggccggga
     1681 cgagggtcca cccgtccatg cccatgacgg ccggctcacc gaagtcgagc acgatcgcca
     1741 ccacccagag cgcttcctgc caaccgctgt cgaggaacgc ggcaccgaac agcaggctga
     1801 cggcgatccc cgagctcagg gcgagcccgg cgaccgaatg gcgcatcgcc gggtcgccgc
     1861 ggctggccag cacgtagagc aggatgtggc cgccccgcac gacggcatag gccacggcga
     1921 aagggaccgc ccggtcgccg aaggcctcgg ggacgcagag ggcgacgatg agcaggccgg
     1981 ccatcgccgc gaacatggcc aggcgaatcg ccccttcttc ggggtcgatc acgctggtca
     2041 gccacgcgta gccgacccag gcccaccaga gcacggccag ggcgagcatg ccccgaccta
     2101 ttcccggcca ggtcggctgt gccgccatca acgccgtgca ctgcgtgaac ccgaggacga
     2161 acacgaggtc gaagaacagc tccagcggct tgaccgactc ctcctggcgc ggctccggct
     2221 ggatccggcc gagccggtcc ccgttgcttc gtggcggggt catccctcca tcgtggcagc
     2281 ggccgtcacc gccgggacgc gcggagctga cgccacgcca cgcgcatccc gacggccgca
     2341 caggtgctga gcgggatcgc cagggacagc agctcctcca gggggacccc gttcacatgg
     2401 gccggcacga cggtggacac cggctcagtc gacccgggcc ggctcgtact cgtcgtggcg
     2461 ccgccaccac atgccctctt cgttgcggcc gagcggcgcc cggtcgagcc actggtacat
     2521 g
//
LOCUS       DASTFV010000652         4131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2461176, whole genome shotgun sequence.
ACCESSION   DASTFV010000652 DASTFV010000000
VERSION     DASTFV010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2461176"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VFP02_11980"
     CDS             complement(<1..572)
                     /locus_tag="VFP02_11980"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9543799.1"
                     /translation="MQEGESGRFTSFDGTGIAFDRWPGERGPVVLHHGFAADGRQNWV
                     RPGIAAKLLDLGFGLVVVDARGHGRSDKSYDPADYGEPVMARDVSALADHLGLESWMQ
                     VGYSMGGIVSLFVAIEDPRVTRLVVGGIGAGVVERGGLDTRVVPRKAVAEALESEGDG
                     AEISPAAARFRAFAESTGADRRALAACARAA"
     gene            666..3149
                     /locus_tag="VFP02_11985"
     CDS             666..3149
                     /locus_tag="VFP02_11985"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9543800.1"
                     /translation="MQRFWSRLAVELGKHAIIVGVVVGLITLVLGAGINQLEFATGQD
                     SYLNKTDQVYKDSVAYQDLFGGQAMLTVISMDEGHTVAELFTPDGIEQFTDFHDALTE
                     SGQTHGVITPLTALEFDDALVASDDGDPTHSIAGAALVRALGEEEEGSPEAEARSADA
                     QVTLERLTAVPEAERTLDNPEWVDFLLFDNQGDIRISLRPFFPDTEHAQVIVRLPGNE
                     DIETEGAAAQLVQDEASKFSFPNSTMVTTGAPVLLRDINDYLRGGMLTLSAIAIGIMI
                     LILLIFFNVRWRLLPMAVILFGVIWAFGLAGYLGIPLTIVTIAGLPVLLGIGIDYAIQ
                     MHARVEEEVILDRADHPIQETARGLGPALLVVTFDAIFAFAALRFAKVPMIRQFGLLL
                     AVGIAVICLASIILPLAALGIREFRSPTTGKDFSEGYLGRFVVRLGSLPAWAAIPLAV
                     ASLAVFLGGLSVEDDLELQTDPIQWVNQESGTIQDIREVEEQVGSSSELGVFAKADDV
                     FTDEFTEFAHDFQNDALAEYPDDLLTASSIEAAVGGLLIVPGASDVAPTGEEVQAAYD
                     VAPEDIQVSTVSPDATAFNMIFQAAPSSLDQRAEVVRQLREETDPPEGISATPSGLAV
                     VGVGLLDNLEANRVVLTYLSILFVFLFLAIRLRSVIRSLLSLVPVLVAVGTASLVAFA
                     FDLKLSPMTAVGGPLVIAACTEFTSLILLRFVEERQRGLEPQEAIDVAGARTGRAFIV
                     SAMTAIAGVAVLSFSSLPLLRDFGRIVAMNVTVALLSALAFLPPMLVWAERRGWVTRG
                     LLKPQEPLLEGPTHAEGVIAPEGIAPAEA"
     gene            complement(3153..3818)
                     /locus_tag="VFP02_11990"
     CDS             complement(3153..3818)
                     /locus_tag="VFP02_11990"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9543801.1"
                     /translation="MYLRRADVQDPEQLATDLGRNVRRLRQRRNLTLEALADRAAVAK
                     GTVIAIEQSRANPNIGTLCRLADALGVGVSALIEAEVGPRVRVKRAEETPALWTGESG
                     GQGLFLMGTDPPEVVELWDWTMAPGETFDGGPHPAGSYEILSVLEGELAVTVGDQEHR
                     LAVGDSVLFDADGAHRYANPGKVTNRYVMTVLEPHPDPALAPPDWLDPEDQAPPSRAG
                     GDP"
     gene            3919..>4131
                     /locus_tag="VFP02_11995"
     CDS             3919..>4131
                     /locus_tag="VFP02_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543802.1"
                     /translation="MAVVFALGAGLSFGTADFVGGLAAKRSPATTVTVLSQVAGFAVL
                     LAVLPFLPGRPSTPALVLGAFAGIAGS"
BASE COUNT          561 a         1546 c         1381 g          643 t
ORIGIN      
        1 gcggcccggg cgcacgccgc cagcgcccgg cgatcggccc cggtcgactc ggcgaaggct
       61 cggaagcggg ctgcggccgg gctgatctcg gcaccgtcgc cctcggactc caaggcctcg
      121 gcgacggcct tgcgggggac gacccgggtg tccaggcccc cgcgctcgac gaccccggcc
      181 ccgatcccgc cgacgaccaa gcgggtgacg cgggggtcct cgatggccac gaacagcgag
      241 acgatgccgc ccatcgagta gccgacctgc atccaggact cgagaccgag gtggtcggcc
      301 agggccgaca cgtcccgggc catgaccggc tcgccgtagt ccgccgggtc gtacgacttg
      361 tcggagcggc cgtggccgcg ggcgtcgacg acgaccaacc cgaacccgag gtcgagcaac
      421 ttggccgcga tcccgggccg gacccagttc tgacgaccgt ccgccgcgaa cccgtggtgg
      481 aggaccaccg ggccgcgctc ccccggccac cggtcgaagg cgatgccggt cccgtcgaag
      541 gacgtgaacc gtcccgactc cccctcctgc atcccacggc ctcccgtgct cgacgaccgc
      601 agcgtaggat ctgccgctct accggggatc cactggagac tccggcgtgt ccctgggggg
      661 aatgcatgca gcggttctgg tcgcgactcg ccgtcgagct gggcaagcac gcaatcatcg
      721 tcggcgtcgt cgtcggtctg atcaccctgg tgctcggcgc gggcatcaac cagctcgagt
      781 tcgccaccgg ccaggacagc tacctcaaca agaccgatca ggtctacaag gacagcgtcg
      841 cctaccagga cctgttcggc ggccaagcca tgctcaccgt gatctcgatg gacgagggcc
      901 acaccgtggc cgagctcttc accccggatg gcatcgagca gttcaccgac ttccacgacg
      961 ccctgaccga gagcggccag acccacggcg tcatcacccc gctgaccgcg ctcgagttcg
     1021 acgacgccct cgtggccagc gacgacggcg acccgaccca cagcatcgcg ggtgccgcgc
     1081 tcgtacgggc gctcggggag gaagaggaag gctctccgga ggcggaggcc cggtccgctg
     1141 acgcccaggt gaccctggag cggctgaccg ccgtacccga ggcggagcgg acgctggaca
     1201 accccgagtg ggtcgacttc ctcctcttcg acaaccaggg cgacatccgc atctcgctgc
     1261 gccccttctt ccccgacacc gagcacgccc aggtcatcgt ccgcctcccc ggcaacgagg
     1321 acatcgagac cgagggcgcc gccgcccagc tcgtgcagga cgaggcgtcc aagttctcgt
     1381 tcccgaactc gaccatggtc acgacgggcg ccccggtcct cctgcgggac atcaacgact
     1441 acctgcgggg cgggatgctc accctgagcg ccatcgccat cgggatcatg atcctgatcc
     1501 tcctcatctt cttcaacgtc cgctggcgcc tgctgccgat ggccgtgatc ctcttcggcg
     1561 tcatctgggc cttcggcctc gccggctacc tcggcatccc gctgacgatc gtcaccatcg
     1621 ccggcctgcc cgtgctgctc ggcatcggca tcgactacgc gatccagatg cacgcccgcg
     1681 tggaggagga ggtcatcctc gaccgagccg accaccccat ccaggagacc gctcgcggcc
     1741 tcggcccggc cctgctggtc gtcaccttcg acgccatctt cgccttcgcc gccctgcgct
     1801 tcgccaaggt cccgatgatc cgccagttcg gcctgctcct ggccgtcggc atcgccgtca
     1861 tctgcctggc gtcgatcatc ctgcccctgg ccgccctcgg catccgagag ttccgctccc
     1921 cgaccaccgg caaggacttc agcgagggct acctcggccg cttcgtggtg cgcctcggct
     1981 cgctcccggc gtgggccgcg atccccctcg ccgtggccag cctcgccgtc ttcctggggg
     2041 gcctgtccgt cgaggacgac ctcgagctcc agaccgaccc gatccagtgg gtcaaccagg
     2101 agtccggcac gatccaggac atccgagagg tcgaggagca ggtgggcagc tccagcgagc
     2161 tcggggtgtt cgccaaggcc gacgacgtct tcaccgacga gttcaccgag ttcgcccacg
     2221 acttccagaa cgacgccctg gctgagtacc ccgacgacct cctcaccgcg tcgagcatcg
     2281 aggccgcggt cggcgggctg ctgatcgtcc cgggcgccag cgacgtcgcg ccgaccggcg
     2341 aggaggtcca ggcggcgtac gacgtcgcgc cggaggacat ccaggtgtcg acggtcagcc
     2401 ccgacgccac cgcgttcaac atgatcttcc aggccgcccc gagctcgctg gaccagcggg
     2461 cggaggtcgt gcgccagctc cgggaggaga ccgacccgcc cgagggcatc tcggccacgc
     2521 cgtcgggcct ggccgtggtc ggggtgggcc tgctcgacaa cctcgaagcc aaccgggtgg
     2581 tgctgaccta cctgtccatc ctgttcgtct tcctcttcct cgccatccgg ctccgctcgg
     2641 tgatccgatc cctgctgtcc ctggtgcccg tgctggtcgc ggtggggacg gcctcgctgg
     2701 tggcctttgc gttcgacctc aagctgagcc cgatgaccgc cgtcggcggg ccgctggtga
     2761 tcgccgcctg cacggagttc acctccctga tcctcttgcg gttcgtcgag gagcgccagc
     2821 ggggcctcga gccccaggag gccatcgacg tggccggggc ccgcaccggt cgggccttca
     2881 tcgtgtcggc catgaccgcc atcgccggcg tcgccgtgct gtcgttctcg tcgctgcccc
     2941 tgctgcggga cttcgggcgc atcgtggcca tgaacgtgac cgtggccctg ctgtcggccc
     3001 tggccttcct gcccccgatg ctggtctggg ccgagcgtcg cggctgggtc acccgcggcc
     3061 tcctcaagcc ccaggagccg ttgctggagg gtccgaccca cgccgagggc gtgatcgccc
     3121 ccgaaggcat cgcccccgcc gaggcgtagc cgctacggat cgccgccggc ccggctgggc
     3181 ggggcctggt cttcggggtc cagccagtcg ggcggcgcca gcgcggggtc agggtggggc
     3241 tcgaggaccg tcatcacgta gcggttggtg accttgccgg ggttggcgta gcggtgggcg
     3301 ccgtcggcgt cgaagaggac gctgtcaccg acggcgaggc ggtgctcctg gtcgccgacg
     3361 gtgaccgcca gctcgccctc cagcaccgag aggatctcgt aggacccggc gggatggggg
     3421 ccgccgtcga aggtctcccc gggcgccatg gtccagtccc agagctcgac cacctcgggc
     3481 gggtcggtgc ccatgaggaa gaggccctgg ccaccggact cgccggtcca caaggccggc
     3541 gtctcctcgg ctcgcttgac ccggacccgg gggccgacct cggcctcgat gagggccgac
     3601 accccgaccc ccagggcgtc ggccaggcgg cacagcgtcc cgatgttggg gttggcccgg
     3661 gactgctcga tggcgatgac ggtgcccttg gccacggcgg cccggtcggc cagcgcctcc
     3721 agcgtcaggt tgcggcgctg tcgcaggcgg cgcacgttgc gccccaggtc ggtggccagt
     3781 tgctcgggat cctgcacgtc ggccctcctc aggtacatct catcatactc aggtggtcac
     3841 tttgctgaac tagcggtaga gtgcacttca ctggactgtc cagtccaatg acctgtacga
     3901 gcgtgaggag tgccggagat ggccgtggtg ttcgccctgg gggccggact gtcgttcggg
     3961 acagccgact tcgtcggcgg cctggccgcc aagcgctccc cggccaccac ggtcacggtg
     4021 ctgtcccagg tcgccggctt cgccgtcctg ctggccgtcc tgccgttcct gcccggccgt
     4081 ccgtcgacgc cggccctcgt gctcggtgcc ttcgcgggca tcgccgggag c
//
LOCUS       DASTFV010000653         2157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2461678, whole genome shotgun sequence.
ACCESSION   DASTFV010000653 DASTFV010000000
VERSION     DASTFV010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2461678"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFP02_12000"
     CDS             complement(<1..266)
                     /locus_tag="VFP02_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543803.1"
                     /translation="MSRTHRALTVLALSLALATATSAVAVAAEDLNAQRKALYQSQLE
                     QNLAGRTAASTQSAVADQNDRLLRGVEAAQAERTRAAVEQARAG"
     gene            501..794
                     /gene="groES"
                     /locus_tag="VFP02_12005"
     CDS             501..794
                     /gene="groES"
                     /locus_tag="VFP02_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET9543804.1"
                     /translation="MGVSLQPLEDRIVVKPSEGEEMTASGLVIPDTAKEKPQEGEVLA
                     VGPGRFEDGTRVPLDVKVGDKVIYSKYGGTEVKLSGDELLILSARDVLAIVNK"
     gene            813..>2157
                     /gene="groL"
                     /locus_tag="VFP02_12010"
     CDS             813..>2157
                     /gene="groL"
                     /locus_tag="VFP02_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET9543805.1"
                     /translation="MPKQLLFSAEARRSLEAGVDKLANVVRVTLGPKGRNVVLDKKWG
                     APTITNDGVTIAKEIELEDHNENLGAQLAKEVATKTNDVAGDGTTTATVLAQALVHEG
                     LRNVAAGANPMGLKRGMEKATEAVAEAIRNQAREIESQEEIASVAAISAQDAEIGKTI
                     AEAMDKVGKDGVITVDESQTFGMELEFTEGMQFDKGYISPYFVTDQERMEAVLEDPYI
                     LIANSKISSAQQLLPVLEKVVQSGKALIVVAEDVDGEALATLVVNKIRGTFKSAAVKA
                     PGFGDRRKAMLADIATLTGGQVISEEVGLKLENATLDLLGRARRLTVTKDDTTIVEGA
                     GSTEDIKGRIAQIKQEIERTDSDWDREKLQERLAKLAGGVGVIKVGAATEVELKEKKH
                     RIEDAVSNARAAVEEGIVPGGGVTLLRVQEALTKVDLTGDEATGATIVRKALEEPL"
BASE COUNT          392 a          750 c          738 g          277 t
ORIGIN      
        1 tcgcccgccc gcgcctgctc gacggcggcc cgggtgcgct cggcctgagc ggcctcgaca
       61 ccgcggagca gccggtcgtt ctggtccgcg accgcggact gggtggaggc ggcggtcctg
      121 cccgccaggt tctgctccag ctgggactgg tacagcgcct tgcgctgcgc gttgaggtcc
      181 tcggcggcca cggcgacggc agacgtcgcg gtggcgagcg ccaggctcag cgccagcacg
      241 gtcagcgccc ggtgggttcg gctcatggcg cccctccttg tgagtctttt cacaagcgtt
      301 tctacgccgc catcgtcccg gcagcgtgac ggcaccatga cgacccgacc aatccgaagt
      361 tttttcgccg gttgacgggg ccggcccgga ttcctaggat ctggttagca ctcgaagaag
      421 ccgagtgcta acatccgcgc cgaacgtgta tcccttcagg cccacccgcc gggtcgggcc
      481 accacgcacg aggaggaagg atgggcgtca gcctgcagcc gctcgaggat cgtatcgtcg
      541 tcaagccgag cgagggcgag gagatgaccg cctcggggct ggtcatcccc gacaccgcca
      601 aggagaagcc ccaggagggc gaggtcctgg ccgtcgggcc cggccgcttc gaggacggca
      661 cccgcgtgcc gctcgacgtc aaggtcggcg acaaggtcat ctactccaag tacggcggca
      721 ccgaggtcaa gctgagtggg gacgagctgc tgatcctctc cgcccgcgac gtcctcgcga
      781 tcgtcaacaa gtaaccgagg tagcgcgaag ccatgccaaa gcaactgctg ttcagcgccg
      841 aggcgcgccg ctcgctcgag gccggggtcg acaagctggc caacgtggtc cgggtgacgc
      901 tcgggcccaa gggccgcaac gtcgtcctcg acaagaagtg gggcgccccc accatcacca
      961 acgacggcgt gaccatcgcc aaggagatcg agctcgagga ccacaacgag aacctcggtg
     1021 cccagctggc caaggaggtc gcgaccaaga ccaacgacgt ggccggtgac ggcaccacca
     1081 ccgccaccgt cctcgcccag gccctggtcc acgagggcct gcgcaacgtg gccgccgggg
     1141 ccaacccgat gggcctcaag cggggcatgg agaaggccac cgaagcggtc gccgaggcga
     1201 tccgcaacca ggcccgcgag atcgagagcc aggaggagat cgcctcggtg gcggccatct
     1261 cggcccagga cgccgagatc ggcaagacca tcgccgaggc catggacaag gtcggcaagg
     1321 acggggtcat caccgtcgac gagtcgcaga ccttcgggat ggagctggag ttcaccgagg
     1381 ggatgcagtt cgacaagggc tacatctcgc cctacttcgt caccgaccag gagcgcatgg
     1441 aggcggtcct ggaggacccc tacatcctca tcgccaactc caagatctcc tcggcccagc
     1501 agctcctgcc ggtgctcgag aaggtcgtgc agtccggcaa ggcgctgatc gtcgtcgccg
     1561 aggacgtcga cggggaggcc ctggccaccc tggtcgtcaa caagatccgc ggcaccttca
     1621 agagcgccgc cgtcaaggcc cccggcttcg gcgaccgccg caaggccatg ctggccgaca
     1681 tcgccaccct caccggtggc caggtcatca gcgaggaggt cggcctcaag ctcgagaacg
     1741 ccaccctcga cctgctcggc cgggcccgcc ggctcaccgt caccaaggac gacaccacga
     1801 tcgtcgaggg tgccgggtcg accgaggaca tcaagggccg catcgcccag atcaagcagg
     1861 agatcgagcg gaccgactcg gactgggacc gcgagaagct ccaggagcgg ctggccaagc
     1921 tggccggcgg cgtcggcgtg atcaaggtcg gcgcggccac cgaggtcgag ctcaaggaga
     1981 agaagcaccg catcgaggac gccgtctcca acgcccgcgc ggcggtggag gagggcatcg
     2041 tccccggcgg cggcgtgacc ctgctgcggg tccaggaggc cctcaccaag gtcgacctga
     2101 ccggcgacga ggcgaccggg gcgaccatcg tccgcaaggc gctcgaggag ccgctca
//
LOCUS       DASTFV010000654         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2463999, whole genome shotgun sequence.
ACCESSION   DASTFV010000654 DASTFV010000000
VERSION     DASTFV010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2463999"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFP02_12015"
     CDS             <1..298
                     /locus_tag="VFP02_12015"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9543806.1"
                     /translation="LPARFSRGLRQKTALLLGLARPFDLLIVDEPFVGLDLAGKAALV
                     ELLEERHQAGATLVVATHDPDYADRVDRCIALRDGDVLFDGPSHKADIAKLVSV"
     gene            375..2198
                     /locus_tag="VFP02_12020"
     CDS             375..2198
                     /locus_tag="VFP02_12020"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HET9543807.1"
                     /translation="MDILGISAYYHDSAAALVRDGELVAAAQEERFTRIKHDAAFPEH
                     AVRYCLDHAGVPAAGLDAVVFYEKPLTHFVRLLRTYLGTAPQGFGSFHRAMPIWLREK
                     LWIGQKIETELKALGYDRPGHLWFAEHHESHAASAFFPSPFESAAVLTFDGVGEWATS
                     SIGVGRGHEVEMVRQLNFPNSLGLLYSAFTYYCGFRVNSGEYKLMGLAPYGEPRFVDA
                     ILDHLIDLHDDGSFTVNRRYFGYIASLRMTNDRFDDVFGGPPRAPESELTHREMDLAR
                     SIQVVTEEVVLRMARTAASLTGERRAVLAGGVALNCVANGRLLREGPFDDVWIQPASG
                     DAGGAVGAALWGWHQVLGHERTPVRPDGMHGAFLGPSWTTDQIAAWLDEQGYPYRRVE
                     PDQRGRAVAELAARGDVVGLFLGRMEFGPRALGHRSIIGDPRSPEMQSVLNLKIKYRE
                     SFRPFAPAVLEERVAEYFDHKGPSPYMLIVADVREERWVPAEEPPDDLREWVNQVRSD
                     LPAVTHVDHSARLQTVDAERNPEFHQILTAFEDLTGCAVLINTSFNVRGEPIVGTPED
                     AYRCFRRTEMDTLVLQDLILEEADQPEWTEGAEWTLEFTLD"
BASE COUNT          320 a          879 c          751 g          340 t
ORIGIN      
        1 cctcccggcc cgcttcagcc gcggcctccg tcagaagacg gccctgctcc tcggcctggc
       61 ccgacccttc gacctgctca tcgtggatga gcccttcgtc ggcctcgacc tggccggcaa
      121 ggcggcgctg gtcgagctgt tggaagagcg ccaccaggcg ggggcgacct tggtcgtggc
      181 cacccacgac cccgactacg ccgaccgggt cgaccgctgc atcgccctgc gcgacggcga
      241 cgtcctcttc gacggcccgt cccacaaggc cgacatcgcc aagctggtgt ccgtctgagc
      301 gtgggtccgg cgcgggccgt ggcccgtcgg atcgcgcgag actgacgctc ggcccggggg
      361 ggccgaccac gaccatggac atcctcggga tcagcgcgta ctaccacgac tcggccgccg
      421 ccctggtgcg ggacggcgag ctcgtggccg cggcccagga agagcggttc acccgcatca
      481 agcacgacgc cgccttcccc gagcacgccg tgcgctactg cctggaccat gccggcgtcc
      541 ccgccgccgg cctcgacgcg gtggtcttct acgagaagcc gttgacccac ttcgtccggc
      601 tgctgcgcac ctacctgggc accgcgcccc aggggttcgg gtccttccac cgggccatgc
      661 ccatctggct gcgggagaag ctctggatcg gccagaagat cgagaccgag ctgaaggccc
      721 tcggctacga ccggccgggc cacctctggt tcgccgagca ccacgagagc catgccgcct
      781 cggccttctt cccgtcgccg ttcgagtccg ccgccgtact caccttcgac ggggtggggg
      841 agtgggccac ctccagcatc ggcgtcggcc ggggccacga ggtcgagatg gtccgccagc
      901 tcaacttccc gaactcgctc ggcctcctct actccgcctt cacctactac tgcgggttcc
      961 gggtcaactc cggcgagtac aagctcatgg ggctcgcccc ctacggcgag ccccgcttcg
     1021 tcgacgccat cctcgaccac ctcatcgacc tccacgacga cggctccttc accgtcaacc
     1081 ggcgctactt cggctacatc gcctcgctgc ggatgaccaa cgaccgcttc gacgacgtgt
     1141 tcggcggccc gccccgggcc cccgagtccg agctcaccca ccgcgagatg gacctggccc
     1201 gctccatcca ggtggtgacc gaggaggtcg tcctgcgcat ggcccgcacc gcggcgtccc
     1261 tcaccggcga gcgccgggcg gtgctggcgg ggggagtggc cctgaactgc gtggccaacg
     1321 ggcgcctgct ccgggagggc ccgttcgacg acgtgtggat ccagcccgcg tccggtgacg
     1381 cgggtggggc ggtcggcgcc gccctgtggg gctggcacca ggtcctcggc cacgaacgca
     1441 cacccgtccg tcccgacggc atgcacgggg ccttcctcgg cccgtcgtgg accaccgacc
     1501 agatcgccgc ctggctggac gagcagggtt acccctaccg gcgggtggag cccgaccagc
     1561 ggggccgggc cgtggccgag ctggccgccc gaggtgacgt ggtgggcctg ttcctggggc
     1621 gcatggagtt cgggccgcgg gccctcggcc accgctcgat catcggtgac ccccggtcac
     1681 cggagatgca gtcggtgctc aacctcaaga tcaagtaccg cgagtcgttc cggcccttcg
     1741 ccccggccgt gctcgaggaa cgggtggccg agtacttcga ccacaagggc ccgtcgccct
     1801 acatgctgat cgtggccgac gtccgggagg agcgctgggt cccggccgag gagccccccg
     1861 acgacctgcg ggagtgggtc aaccaggtcc gttccgacct gcccgccgtc acccacgtcg
     1921 accactcggc ccgcctccag accgtggacg ccgagcgcaa cccggagttc caccagatcc
     1981 tcaccgcctt cgaggacctg accggctgcg ccgtgctcat caacacgtcg ttcaacgtcc
     2041 ggggcgagcc catcgtgggc acgcccgagg acgcctaccg ctgcttccgg cgcaccgaga
     2101 tggacaccct ggtcctccag gacctcatcc tggaggaggc ggaccaaccc gagtggaccg
     2161 aaggggccga atggaccctc gagttcaccc tcgactgatg atcttcctcc gcaacgccgg
     2221 tcgcctgacc cgggacgtcg tcggctacgc cgtgcacaac cgggcctggt ggctggtgcc
     2281 cgtcatgggc
//
LOCUS       DASTFV010000655         4067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2467009, whole genome shotgun sequence.
ACCESSION   DASTFV010000655 DASTFV010000000
VERSION     DASTFV010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2467009"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="VFP02_12025"
     CDS             complement(<1..122)
                     /locus_tag="VFP02_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9543808.1"
                     /translation="MAGGEGTRLRPLTSNLPKPMMPLANKPMMEHVIDLLRRHG"
     gene            complement(134..748)
                     /locus_tag="VFP02_12030"
     CDS             complement(134..748)
                     /locus_tag="VFP02_12030"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9543809.1"
                     /translation="MEDAAAGEDRILTIPNVISVVRLLCVPVFLWLLFDQENRVAAAG
                     LLAILGATDWVDGYIARHYDQVSNLGKILDPVADRVLLIVGVVAILIDGAAPLGVGIL
                     SLLREGLVTVAVLVLAACGAARIDVTWYGKAGTFSVMFAFPLFLWASAGDDTVNDLAY
                     VAAWGFAIPGLILGYIALAQYVPLGLQALRDGRADRAELPEVPA"
     gene            782..1201
                     /locus_tag="VFP02_12035"
     CDS             782..1201
                     /locus_tag="VFP02_12035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET9543810.1"
                     /translation="MASVFTMIIEGQIPGEFVWRDDRCVAFLSINPLHDGHTLVVPRD
                     EVDHWVDLDDDLAAHLFVVARKIGKVQQDLFDPLRVGLMIVGEEVPHAHLHVVPIDRV
                     ADLDFSNAAPGPGDDFAEIAQRLRAALAAAGHDEATA"
     gene            complement(1180..2688)
                     /locus_tag="VFP02_12040"
     CDS             complement(1180..2688)
                     /locus_tag="VFP02_12040"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HET9543811.1"
                     /translation="MSQRRELPLKRRLDLLTLRLQGRLDSEGADRVLPWAFFVGLFAL
                     LAGFALARARGLEEGADLGTYIQGAWLITRGDAPFLTIRGTHLLAEQAGFSFYPIAGL
                     TSFLPTIPTLLVIQSGLLALGVVPLWRIARRVADLRVGASLVLLSAFALYPAIHAMNT
                     AGFNPAVLALPALLGMALFGLTGRWWLFSACAVVAVLTRADLALVTAAFGGLLVLEGR
                     RRAGLITMGVSVAYVLLALLVIQPYYNDGAFVHAIAFEHYGSSPLGALGGLLAHPFRA
                     VGDLIDQQNFEILVMLFAPVFFLPFVAPRYLLPVVPLECLYLIANVSEDRVPRPEHAV
                     AITAFIFVATAIGLSRIGRRSVERVNVDRRVLIALVFASAVFFVQDSPASPYQEPWAW
                     GVRDEADQARLDAADLVPDRDSVRASPTLLPLLAERAAVYELDTIDNPHVRRAADGVD
                     AILLDAEAAPDWDDDDRRRFQQGLVELGFDEVFAEAGITLYERNDPQAVASS"
     gene            complement(2685..3392)
                     /locus_tag="VFP02_12045"
     CDS             complement(2685..3392)
                     /locus_tag="VFP02_12045"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HET9543812.1"
                     /translation="MCGRFVSTSPPDEIAKYFGATEVDERLEGPNYNVAPTQDVYAVL
                     ENGGVRRVESLHWGLIPFWAKEAKVGNKMINARAETIATSGAFKHAFKKRRCIIPVDG
                     FYEWAKAEGQAKKQPWFIHRPDDEPMAFAGLWEVWRPEGSEDELVSCTIITGEPNEKM
                     AEIHDRMPVMLPPDAWDRWLDRDEQDMALLGKLLVPAPSSLITLHPVSTEVNNVRNQG
                     EHLTDPIELDEPPLTEG"
     gene            3473..3784
                     /locus_tag="VFP02_12050"
     CDS             3473..3784
                     /locus_tag="VFP02_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543813.1"
                     /translation="MPQSEPNSARGAKKGPTPHRTLDQARVGARDKVKQARRALVELE
                     RRHDRAAAKVEALSRRKKALDLELGDAEAERDRTKSRLAATKQALESAEARLIALERE
                     N"
     gene            complement(3781..>4067)
                     /locus_tag="VFP02_12055"
     CDS             complement(3781..>4067)
                     /locus_tag="VFP02_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543814.1"
                     /translation="IRVERIFDAPRTHVYLHDEADEWFRQALAFHEAMEAPFFVAMTQ
                     TAWADLLADRDGSGDAGRARTLAGTALPVAQERGYGYVERDARSVIERIG"
BASE COUNT          671 a         1376 c         1455 g          565 t
ORIGIN      
        1 aagccgtgac gccgcagtag gtcgatgacg tgctccatca tcggcttgtt ggccagcggc
       61 atcatcggct tgggcaggtt ggaggtcagg ggccgcagcc gggtgccctc gccgcccgcc
      121 atgatcaccg ctttcacgcc ggcacctcgg ggagctcggc ccggtcggcc cttccgtcac
      181 gcagtgcctg gagcccgagg gggacgtact gggccagggc gatgtagccc aggatcagcc
      241 cggggatggc gaagccccag gccgccacgt aggcgaggtc gttcaccgtg tcgtcgccgg
      301 cgctcgccca caggaacagg gggaaggcga acatcactga gaacgtgccg gccttgccgt
      361 accacgtgac atcgatgcgg gcggctccgc acgcggcgag gacgagcacg gcgacggtga
      421 ccaggccttc gcgcagcagc gacaggatcc ccacccccag cggggcggca ccgtcgatca
      481 ggatggcgac caccccgacg atgaggagca cgcggtcagc caccgggtcc aggatcttgc
      541 cgaggttcga cacctggtcg tagtggcggg cgatgtagcc gtcgacccag tcggtggcgc
      601 cgaggatggc caggaggccg gccgcggcca ctcggttctc ctggtcgaac agcagccaga
      661 ggaaaacggg cacgcagagc aggcgcacca cggagatgac gttggggatc gtgaggatgc
      721 ggtcctcccc cgctgccgcg tcctccatgg ccgcccaggg tagatcggct acaaccaccc
      781 catggcttcc gtgttcacca tgatcatcga ggggcagatc ccgggcgagt tcgtctggcg
      841 ggacgaccgg tgcgtcgcct tcctgtcgat caatccgctc cacgacggcc acacgctggt
      901 cgtcccccga gacgaggtcg accactgggt cgacctggac gacgacctcg ccgcccacct
      961 cttcgtggtc gcccgcaaga tcgggaaggt ccagcaggac ctgttcgacc cgctgcgggt
     1021 cggtctgatg atcgtcggcg aggaggtccc gcatgcgcac ctgcacgtcg tacccatcga
     1081 ccgggtcgcg gatctcgact tctcgaacgc cgcccctggt cccggcgacg acttcgccga
     1141 gatcgcgcaa cgcctccgcg ccgccctggc tgcggcgggt cacgacgaag cgacggcctg
     1201 agggtcattg cgttcgtaga gggtgatgcc ggcctcggcg aagacctcgt cgaaacccag
     1261 ctccaccagg ccctgctgga agcggcggcg gtcgtcgtcg tcccagtcgg gcgcggcctc
     1321 ggcgtccagg aggatggcgt cgaccccgtc ggcggcccgg cgcacgtggg ggttgtcgat
     1381 cgtgtccagc tcgtagacgg cggcgcgttc ggccagcagg gggaggaggg tcggcgaggc
     1441 ccgcaccgag tcccggtcgg gcaccaggtc ggcggcgtcc aggcgggcct ggtcggcctc
     1501 gtcccgcacc ccccaggccc acggttcctg gtacggcgac gccggcgagt cctgcacgaa
     1561 gaacacggct gacgcgaaga cgagggcgat gagcacacgg cggtccacgt tgacccgctc
     1621 gacgctccgg cggccgatcc tcgagaggcc gatggcggtg gccacgaaga tgaaggccgt
     1681 gatggccacg gcgtgctcgg ggcgggggac ccggtcttcg gacacgttgg cgatcaggta
     1741 gaggcattcc agcggaacca cgggcaggag gtaccggggg gccacgaagg ggaggaagaa
     1801 gaccggggcg aagagcatca ccaggatctc gaagttctgc tgatcgatga ggtcccccac
     1861 cgcccggaac ggatgggcca gcaggccgcc gagcgccccc agcgggctgg agccgtagtg
     1921 ctcgaaggcg atggcgtgca cgaaggcgcc gtcgttgtag tacggctgga tcacgagcag
     1981 ggcgagcagg acgtaggcca cggacacgcc catcgtgatc aggcctgccc ggcgccggcc
     2041 ctccagcacc aggagcccgc cgaaggcggc ggtgaccagg gccagatccg ctcgggtgag
     2101 cacggcgacc acagcacagg cgctgaagag ccaccaccgt cccgtgaggc cgaacagggc
     2161 catgcccagc agggcgggaa gagccaggac ggcgggattg aaccccgccg tgttcatggc
     2221 gtggatggcc gggtagaggg cgaaggcgct gagcaggacg agcgaggccc cgacccggag
     2281 gtccgccacc cggcgggcga tgcgccacag tggcaccacg ccgagggcca gcaggccgga
     2341 ttggatcacc agcagggtcg ggatggtggg caggaacgag gtcagcccgg cgatcgggta
     2401 gaagctgaac ccggcctgtt cggccagcag gtgggtcccg cggatggtca ggaaaggcgc
     2461 atccccgcgg gtgatgagcc aggccccctg gatgtaggtc ccgaggtcgg ctccttcctc
     2521 gaggccgcgg gcccgggcca gggcgaaacc ggccagcagg gcgaacaggc cgacgaagaa
     2581 ggcccagggc agcacccgat ccgcgccctc gctgtccagg cgaccctgga gccgcaacgt
     2641 gagcaggtcg aggcgtcgct tgagcgggag ctcccgccgc tggctcatcc ctcggtcagg
     2701 ggaggctcgt ccagctcgat cggatccgtc aggtgctcac cctggttgcg gacgttgttg
     2761 acctcggtgc tgaccgggtg gagcgtgatc agcgaggagg gggcgggcac caggagcttg
     2821 cccagcaggg ccatgtcctg ctcgtcgcgg tccagccacc ggtcccaggc gtcagggggc
     2881 agcatcaccg gcatccggtc atggatctcg gccatcttct cgttgggctc gccggtgatg
     2941 atggtgcagg acaccagctc gtcctcactg ccctccggtc gccagacctc ccacaggccg
     3001 gcgaaggcca tgggctcgtc gtcgggtcgg tggatgaacc acggctgctt cttggcctgc
     3061 ccctcggcct tggcccactc gtagaagccg tcgaccggga tgatgcagcg ccgcttcttg
     3121 aaggcgtgct tgaaggctcc actggtcgcg atggtctcgg ctcgcgcgtt gatcatcttg
     3181 ttcccgacct tggcctcctt ggcccagaac gggatcagcc cccagtgcag cgactccacc
     3241 cggcgcacgc ccccgttctc caggacggcg tacacgtcct gggtcggggc cacgttgtag
     3301 ttcgggccct cgaggcgctc gtcgacctcg gtggcaccga aatacttggc gatctcgtcg
     3361 ggcggggagg tcgagacgaa ccggccgcac acagccggtc acggtagcgg tcgacccgaa
     3421 ggcggggggt agcgagtcgc aaagtggacc aatcccgagc acaatcgttg acttgcccca
     3481 gtccgaaccc aactctgccc gtggcgccaa gaagggtcct acgcctcatc gcacgctgga
     3541 tcaggcgcgg gtcggggccc gtgacaaggt caagcaggcc cgtcgggccc tcgtcgaact
     3601 ggagcgccgg cacgaccgtg cggcggccaa ggtcgaggcg ctgagccgcc gcaagaaggc
     3661 cctcgacctc gagctgggcg acgccgaagc cgagcgggac cgcaccaagt cccgcctcgc
     3721 cgccaccaag caggccctcg aatccgccga ggcccgcctc atcgccctgg agcgtgagaa
     3781 ctagccgatc cgttcgatca ccgagcgggc atcgcgttcg acgtagccgt agccgcgctc
     3841 ctgagcgacg ggtagcgcgg tgccagcgag ggtccgagcc cggcccgcgt ctcccgaccc
     3901 gtcacggtcg gcgaggagat ccgcccatgc ggtctgggtc atggcgacga agaacggcgc
     3961 ttccatcgcc tcgtggaacg ccagcgcctg cctgaaccac tcgtcggcct cgtcgtggag
     4021 gtagacgtgc gtccgcggtg cgtcgaagat ccgctcgaca cggatct
//
LOCUS       DASTFV010000656         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2467682, whole genome shotgun sequence.
ACCESSION   DASTFV010000656 DASTFV010000000
VERSION     DASTFV010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2467682"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VFP02_12060"
     CDS             complement(<1..974)
                     /locus_tag="VFP02_12060"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HET9543815.1"
                     /translation="MARFEPFRGLRYDDERLDYGEVTAPPYDVIGDAERAELGAKHPH
                     QAVHLDLPLDDGDTSRYDVACRTLGEWRDEGVLIQDEDPSFYVYRMGYKDDTGRLRQT
                     AGVIGALELAQPGTGGVLPHEQTTPKAKSDRLEMLRYCKANLSAVWGLSTAKGLSGLC
                     ELPGPPVAAWTDGEGVHHRLWPISQPGIVEAIASAVSSEPVVIADGHHRWSTSLQYRD
                     ERHEAGDGAGPWDLAMTYVVELVEEQLMVQPIHRLLNGLPEGFDLAAALEASFELFDA
                     GPVGPELLARRDDAGALCLVTRDGAQLLRPRPEVMEGVRDLDSSRLDQG"
     gene            1036..1776
                     /locus_tag="VFP02_12065"
     CDS             1036..1776
                     /locus_tag="VFP02_12065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HET9543816.1"
                     /translation="MAWALDLDGVVWLGDQPIPGASDAVGRLQASGESVVFVTNNSSV
                     PVGEVEAKLARQGIAAAGAVLTSAMAAAELVQPGEVALVCAGPGVDEALAARGVHTVR
                     DGKADVVVVGFHRDFDYGRLQAASTAVRTGARLLATNDDATYPTPDGLIPGGGAILAA
                     VSTASGVHAEVAGKPYQAMADLVRARVGAHGVMVGDRADTDGRFALALGYRFALVLSG
                     VTTELPVLPEPDLVAADLAGLVDQELSR"
     gene            1773..>2589
                     /locus_tag="VFP02_12070"
     CDS             1773..>2589
                     /locus_tag="VFP02_12070"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9543817.1"
                     /translation="MRRAVPVVGMLLVAGLLAGCASDVPESEPTRPADALTWTTEVGE
                     EIDGLPIAVGSQSRPVDLLLGWIATEALVAAGADVTDDLNLGDTQTTRDAQLAGLIDL
                     YWETTGTGWLALLREIGPSADPEQLYEDVRDEDLEENGIVWLPPAPADTGVGIVASPG
                     VIEDRGIVDLSALAAALEDPDEGLKVCVSAVDQPLDSSGLGALADAADVRIRPRLVNP
                     VSPSEIIELTEEGTFCPFALVDRLNVGLADADVTFLNDDLGAFVAEQPSVTIRE"
BASE COUNT          340 a          921 c          957 g          371 t
ORIGIN      
        1 ccctggtcga gcctgctgct gtccaggtcc cggacccctt ccatgacctc gggacgagga
       61 cggagcagtt gagcgccgtc tcgggtcacc aggcagagcg cgccggcgtc atccctgcga
      121 gcgagcagct cgggacctac cgggccggcg tcgaacagct cgaaggacgc ctccagcgcc
      181 gcggccaggt cgaagccctc gggtagtccg ttgaggagac ggtggatggg ctggaccatg
      241 agctgctcct cgaccagctc gaccacgtag gtcatggcca ggtcccaggg tccggccccg
      301 tcgccggcct cgtggcgctc gtcccggtac tgcagcgagg tcgaccagcg gtggtgcccg
      361 tcggcgatga cgacgggctc ggagctgacg gcggaggcga tggcctcgac gatcccgggc
      421 tggctgatcg gccagaggcg gtggtggacg ccctcgccgt cggtccaggc ggccaccggc
      481 ggtcccggca gctcacagag gccggacagc cccttggcgg tggacaggcc ccacaccgcg
      541 gagaggttgg ccttgcagta gcgcagcatc tccagccggt cgctcttggc cttgggcgtg
      601 gtctgctcat gggggagcac gccgccggta ccgggctggg ccagctccag ggccccgatg
      661 acgcccgcgg tctggcgcag gcgacccgtg tcgtccttgt agcccatgcg gtagacgtag
      721 aaggacgggt cctcgtcctg gatcaggaca ccctcgtccc gccactcacc cagggtccga
      781 caggccacgt cgtaccgact ggtgtcgccg tcgtcgagcg ggaggtcgag gtggacggcc
      841 tggtgggggt gcttggcccc cagctcggcc cgctcggcgt cgccgatcac gtcgtagggc
      901 ggagccgtga cctcgccgta gtcgagccgt tcgtcgtcgt agcggagacc ccggaagggc
      961 tcgaagcgcg ccatggggcc catcgtggca ggcgcgggat cggactgggc agtccggtcg
     1021 tgcgaacatg ccgccgtggc ctgggcgctg gatctcgacg gcgtggtctg gctcggggac
     1081 cagcccatcc cgggggcgtc cgacgccgtc gggcggctcc aggccagcgg ggagtcggtg
     1141 gtcttcgtga ccaacaactc ctcggtcccg gtgggggagg tcgaggccaa gctggcccgc
     1201 cagggcatcg cggcggcggg agcggtgctg acgtcggcca tggccgcagc cgagctcgtg
     1261 cagcccggcg aggtggcgct ggtctgtgcc ggaccgggtg tcgacgaggc cttggccgcg
     1321 cggggcgtgc acaccgtccg ggacgggaag gccgacgtgg tcgtggtcgg cttccaccgc
     1381 gacttcgact acggccgatt gcaggccgcc agcaccgccg tgcggaccgg tgcccggctc
     1441 ctggccacca acgacgacgc cacctatccg acccctgacg gtctgatccc cggcggaggg
     1501 gccatcctgg ccgcagtgag cacggcgtcg ggggtccatg cggaggtggc gggcaagccc
     1561 taccaggcca tggccgacct cgtgcgggcc cgggtgggtg cccacggggt gatggtgggc
     1621 gaccgggccg acaccgacgg acgcttcgcc ctcgcgctcg gctaccgctt cgccctcgtt
     1681 ctcagtggcg tgaccaccga gctccccgtc ctgcccgagc ccgacctggt cgccgccgac
     1741 ctggccggcc tcgtcgacca ggagctgagc cgttgaggcg cgccgtcccc gtcgtgggga
     1801 tgctgctggt ggccgggctg ctcgccggct gcgcgtcgga cgtgcccgag tccgaaccca
     1861 cccggcccgc ggatgccctc acctggacca cggaggtcgg cgaggagatc gacggcctgc
     1921 ccatcgccgt cggcagccag tcccgacccg tcgacctcct gctgggctgg atcgccaccg
     1981 aggcgctggt ggccgccggc gccgacgtca ccgacgacct gaacctggga gacacccaga
     2041 ccacccgcga cgcccagctg gccggcctga tcgacctgta ctgggagacc acgggcacgg
     2101 gttggttggc cctgctgcgg gagatcggtc cgagcgcgga ccccgagcag ctgtacgagg
     2161 acgtgcggga cgaggacctg gaggagaacg ggatcgtctg gctgccgccg gctccggccg
     2221 acaccggcgt cggcatcgtg gcctcgccgg gtgtcatcga ggaccggggc atcgtcgacc
     2281 tcagcgcgct cgccgcggcg ctggaggacc cggacgaagg cctcaaggtc tgcgtctcgg
     2341 ccgtcgatca gccgctcgac tcctccgggc tgggcgccct ggccgatgcc gccgacgtcc
     2401 gcatccggcc ccgcctggtc aacccggtct ctccgagcga gatcatcgag ctgaccgagg
     2461 aggggacctt ctgcccgttc gccctcgtcg accgcctgaa cgtcgggctg gctgacgccg
     2521 acgtcacctt cctcaacgac gacctcggcg ccttcgtggc cgaacagccg tcggtgacga
     2581 tccgggagg
//
LOCUS       DASTFV010000657         2849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2476110, whole genome shotgun sequence.
ACCESSION   DASTFV010000657 DASTFV010000000
VERSION     DASTFV010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2476110"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..2818
                     /gene="infB"
                     /locus_tag="VFP02_12075"
     CDS             65..2818
                     /gene="infB"
                     /locus_tag="VFP02_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET9543818.1"
                     /translation="MPSNVRVYELARDLGMSNQEVLDLCEKLGIGARSHSSSIVEAQG
                     DRVRRRAHKDGLVKAAPPEEAKPVEEAKPKNPPVSTKKAPPKKKAEPKAEKAVPEPAP
                     TTAPEPEAPAEVVEAPEPAPAPEVEAPAAEAPPADAPKRVVRSSGSSGPTRPVEPPPA
                     PPTLPASERVRPAAPPASAPAAPAASAAPAAAGAPPASDTGKAIPPPARPPTSSTGKP
                     IPPPPGTGGRGGAPPSRPGARTGGPPRARPGGAPRPGMGAGGGPRPSVPGAGPGGGNR
                     PGGGPPGRGGPQRPQRRRRSRRRRVREELQPIDRPTYTPTDAAVPEGEVVIERTSAPM
                     DIAPKLNRTAADVVRFLMQQGEMVTATQSLSDEMIELFAAEIGAEVRLVDPGEEQEVE
                     LQKLLEYDDWDDEEDEDPDAVERAPVVTVMGHVDHGKTLLLDKIRSSNVTEGEAGGIT
                     QAIGAYQVNRDGHEITFIDTPGHEAFTAMRARGADATDIVILVVAADDGVMPQTVEAL
                     NHAKAADVPIIVALNKVDRENADVQRVMGQLTEHELIPESYGGDTIVVETSALQDLGI
                     DDLLEQLLVVAELEDLRANPEGRAAGVVLESHLDTGRGPVATVLVQKGTLAVGDPVVA
                     GAAAGKVRALIDDHGQQIKAAGPSTPVEVLGLSDVAAAGTGFVVAPDEKVARTVSDKR
                     EHFARQASLGRDAGVATGAKLEDIFSQIQAGEAATLNLVIKADTNGSMEAVTDALRKL
                     ERDEVKLAFVHRGVGGITENDINLAAAANATILGFNVRPERKARQLAEQEGVEIRTYE
                     VIYALLEDIESAMVGMLEPEFEEVVTGDAEVREIFRVPKIGAIAGCYVLNGTITRGSK
                     VRFLREGTIIWKGTITSLRRFKDDVREVQAGFECGIGLSDFQNLQAGDIIETFEEREI
                     PRA"
BASE COUNT          458 a         1095 c          967 g          329 t
ORIGIN      
        1 gcgtgaccag gtgctcgacc gggcagacgc ccgccagaag ctacgaaagg gctgacacag
       61 ccaggtgcca tcgaacgtac gggtctacga actcgcccgg gacctcggga tgagcaatca
      121 ggaggtcctc gacctctgtg agaagttggg tatcggcgcg cgcagccact cctcgagcat
      181 cgtcgaggcc cagggcgacc gtgtccggcg ccgggcacac aaggacggcc tcgtgaaggc
      241 cgcgccgccc gaagaggcca agccggtgga agaggccaaa cccaagaacc cgccggtcag
      301 caccaagaag gcgccaccca agaagaaggc cgagcccaag gccgagaagg ccgtgcccga
      361 gccggcgccc acgaccgcgc cggagcccga ggctcccgcc gaagtcgtcg aggcccccga
      421 gcccgctccg gcccccgagg tcgaggcccc cgcggcggag gctccgcccg ccgacgcccc
      481 caagcgcgtc gtgcgctcga gcggcagcag cggcccgacc cgtcccgtgg aaccgccccc
      541 cgccccgccg accctgcccg ccagcgagcg ggtacggccc gccgcgccac cggcctcggc
      601 ccccgccgcc cctgcggctt cggccgcccc cgccgccgcc ggcgcccccc cggcttcgga
      661 cacgggcaag gcgatcccac ccccggcccg gccgcccacg tcgtcgaccg gcaagcccat
      721 cccgccgccg cccggcaccg gaggccgcgg tggcgcgcca ccgtcccgtc ccggcgctcg
      781 taccggcggt ccgccccgcg ctcgccccgg tggcgccccg cgccctggca tgggtgccgg
      841 cggcggtccc cgtccgtccg ttcccggtgc cggacccggt ggcggcaacc gccccggcgg
      901 tggccctcct ggccgcggtg gcccccagcg gccgcagcgt cggcgccgga gccgtcgtcg
      961 tcgggtccgc gaggagctgc agccgatcga ccggccgact tacacgccga ccgacgccgc
     1021 cgtccccgag ggcgaggtcg tcatcgagcg cacctcggct cccatggaca tcgcccccaa
     1081 gctgaaccgg accgcagccg acgtcgtccg attcctcatg cagcagggcg agatggtcac
     1141 ggccacccag tccctcagcg acgagatgat cgagctgttc gcggccgaga tcggcgccga
     1201 agtccgcctc gtcgatcccg gcgaggagca ggaggtcgag ctccagaagc tcctggagta
     1261 cgacgactgg gacgacgagg aggacgagga ccccgacgcc gtcgagcgcg cccccgtcgt
     1321 caccgtcatg ggccacgtcg accacggcaa gacgctgctg ctggacaaga tccgctccag
     1381 caatgtgacc gagggcgagg ccgggggcat cacccaggcc atcggcgcct accaggtcaa
     1441 ccgtgacggc catgagatca ccttcatcga cacccctggc cacgaggcgt tcaccgccat
     1501 gcgggcccgg ggcgccgacg ccaccgacat cgtcatcctc gtggtggcgg ccgacgacgg
     1561 cgtcatgccc cagaccgtcg aagcgttgaa ccacgccaag gcagccgacg tgccgatcat
     1621 cgtcgccctc aacaaggtcg accgcgagaa cgccgacgtt cagcgggtca tgggccagct
     1681 caccgagcac gagctcatcc ccgagtccta cggcggcgac acgatcgtgg tcgagacgtc
     1741 cgcgctgcag gacctcggca tcgacgacct gctcgagcag ctgctggtgg tcgccgagct
     1801 ggaggacctc cgggccaacc ccgagggccg cgccgccggc gtcgtcctcg agtcccacct
     1861 ggacaccggc cggggccccg tggccactgt cctggtccag aagggcacgc tggccgtcgg
     1921 cgacccggtc gtcgccggcg ccgccgcggg caaggtccgg gccctgatcg acgatcacgg
     1981 ccagcagatc aaagcggccg gcccgtccac gccggtcgag gtcctcggcc tgtccgacgt
     2041 ggccgccgcg ggcaccggct tcgtggtcgc cccggacgag aaggtggccc gcaccgtctc
     2101 ggacaagcgg gagcacttcg cccgccaggc cagcctcggc cgggacgccg gtgtcgccac
     2161 cggggccaag ctggaggaca tcttcagcca gatccaggcc ggcgaggccg ccaccctgaa
     2221 cctggtcatc aaggccgaca ccaacggctc gatggaggcc gtcaccgacg ccctgcgcaa
     2281 gctggagcgg gacgaggtga agctggcctt cgtccaccgg ggggtcggtg gcatcacgga
     2341 gaacgacatc aacctggccg cggcggcgaa cgccaccatc ctcggcttca acgtccgccc
     2401 cgagcgcaag gcccgtcaac tggccgagca ggagggcgtc gagatccgga cctacgaggt
     2461 catctacgcc ctgctcgagg acatcgagtc ggccatggtg ggcatgctcg agcccgagtt
     2521 cgaggaggtc gtcaccggcg acgccgaggt ccgggagatc ttccgggtcc ccaagatcgg
     2581 cgccattgcc ggctgctacg tcctcaacgg gaccatcacc cgggggtcga aggtgcgctt
     2641 tctgcgggag ggcacgatca tctggaaggg caccatcacc tcgctgcggc gcttcaagga
     2701 cgacgtgcgg gaggtccagg ccggcttcga gtgcggcatc ggcctctcgg acttccagaa
     2761 cctgcaggcg ggggacatca tcgagacctt cgaggagcgc gagatcccgc gcgcctgact
     2821 gccgtgtacg tcctcgcgct ggacatcga
//
LOCUS       DASTFV010000658         3606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2480590, whole genome shotgun sequence.
ACCESSION   DASTFV010000658 DASTFV010000000
VERSION     DASTFV010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2480590"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..469)
                     /locus_tag="VFP02_12080"
     CDS             complement(29..469)
                     /locus_tag="VFP02_12080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET9543819.1"
                     /translation="MDEPAADPAAPAPGPITPAVVAVVVTHEPGTWFDEGLRALAAQD
                     YPDLTVLVVDAGSTVDPTERVAAACPTAFVRRIDTNPGFGAAANEALETVEGASYLLV
                     CHDDIALSPGAVTASSRRRAGPTPAWSGPRCPSGTTPASCWRWA"
     gene            600..779
                     /locus_tag="VFP02_12085"
     CDS             600..779
                     /locus_tag="VFP02_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543820.1"
                     /translation="MPSSLLTAPVHRTLILAREALYVTVGAGVLAVQRVQVQRRELER
                     ELGRRFSGSPGPEPG"
     gene            776..1906
                     /locus_tag="VFP02_12090"
     CDS             776..1906
                     /locus_tag="VFP02_12090"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET9543821.1"
                     /translation="MRLRVAVDATPLLRRPTGVGGVTRALLERLGRRSDVAALAFAVS
                     WTGRGHLAELLPPGVTTVGRPMAARPLRALWHRVNWPPIELWTGAVDVVHGPNFVVPP
                     SRRAARVVTVHDLTAVRFPELCTSDVLAYPDLLRRALASGAWAQVPSAAVAEEVLEHF
                     AVPADHVVVVANGVDDVPTAPEGAGRARAGRERYVLALGTFEPRKDLPTLVAAFDAAA
                     NDDPDVGLVLAGADGWGAEAVHTAVANSPHRERIRITGWLDDQARAALLREATVLAYP
                     SLYEGFGLPPLEAMGVGVPVVATRVGALPEVLGDAADLVPPSDVDALAQALVRVLTDE
                     AHRAAMVVRGHRRADRYSWEAHADGLVALYRRAAASAPGAAP"
     gene            1917..2822
                     /locus_tag="VFP02_12095"
     CDS             1917..2822
                     /locus_tag="VFP02_12095"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9543822.1"
                     /translation="MRALVTGAAGFVGRHLVAGLQEAGDDVHETDRHLGLDILDPAGL
                     TDLFTSVRPEVVYHLAGQADVGGSWTSPVETFRTNAEGTLNVLYAAVQAGVERVVAVA
                     SADVYGQVPEADLPIGEDRAFRPVSPYGASKAAADVIALQAYLGHGLAVLRARPFNHL
                     GPGQSDRFVTSAVASRIARNEVSGSDEVPVGNLSARRDFTDVRDVVRAYRLLAERGQP
                     GEAYNVCSGRDIAVQEVADRLIALAERPMALVPDPDLLRPVDTPVLRGDPTRLHEATG
                     WEPTISLDQTLADVLDDWRKRVRET"
     gene            complement(2836..3309)
                     /locus_tag="VFP02_12100"
     CDS             complement(2836..3309)
                     /locus_tag="VFP02_12100"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9543823.1"
                     /translation="MARELERNVLGRRIADHRAKQGWTQADLAERLGVSRVTISHVEA
                     GMSYPGERTVTLLAGLFKLEPHELVAGTAYPTAKADRLPVVANRYTEAEQQVALCETD
                     LAWISRGPSLYGDQRLAQWDVALRRLAQECDRHDRDLVEGCRGKVRRALDERSAG"
     gene            3395..>3606
                     /locus_tag="VFP02_12105"
     CDS             3395..>3606
                     /locus_tag="VFP02_12105"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9543824.1"
                     /translation="MPRRALITGITGQDGSYLAEHLLDHGYEVVGMVRRSSTVNFERI
                     AHIQDRITIVPGDVLDEISMIEILST"
BASE COUNT          456 a         1358 c         1290 g          502 t
ORIGIN      
        1 gacgacgcgt ggccggtgcg gtcgacggtc atgcccatcg ccagcagctg gcgggggtcg
       61 tcccactcgg acaccttggg cccgaccagg ccggcgttgg accggcccgc ctcctcgacg
      121 aggccgtgac ggcgccggga ctgagcgcga tgtcgtcgtg gcagacgagc aggtagctgg
      181 ccccttcgac ggtctcgagg gcctcgttgg cggcggcacc gaatccgggg ttggtgtcga
      241 tccggcggac gaaggccgtg gggcaggcgg cggccacgcg ctcggtcgga tcgacggtgc
      301 tgcccgcgtc gacgacgagc acggtgaggt ccgggtagtc ctgggcggcg agcgcgcgca
      361 ggccctcgtc gaaccacgta ccgggttcgt gggtcaccac gaccgcgacg acggcgggcg
      421 tgatcggacc gggggcaggc gccgcgggat cggcggcggg ttcgtccacg atgcgcgcag
      481 gctagcggtc gcactccggc cccagggggc acccgcgggc cgttcgtcac atcccgagaa
      541 cgccccgacg gccgctcggg ccgggtttgc ttgctctggt tagcagttag gctgcgccca
      601 tgccgtcgtc cctgttgacc gcgcccgtgc accgcacgct gatcctggcc agggaggccc
      661 tctacgtgac cgtgggcgcc ggcgtgctcg ccgtccagcg ggtacaggtc caacgccggg
      721 agctcgagcg cgagctcggc cgtcggttct ccggatcccc cggacccgag ccgggatgag
      781 gctccgggtc gcggtcgacg cgacccccct gctcaggcgg cccacgggcg tcggtggcgt
      841 gacccgggcc ctgctcgaac gactcgggcg gcgctccgac gtcgccgccc tggccttcgc
      901 cgtctcctgg accggtcggg gccacctggc cgagctgctc ccgcccgggg tcacgaccgt
      961 tggtcgaccg atggccgccc gaccgctgcg ggcgctgtgg caccgggtca actggccgcc
     1021 catcgagctc tggaccggag ccgtcgacgt ggtccacggg ccgaacttcg tcgtcccccc
     1081 gagccggcgg gcggcccggg tggtcacggt ccacgacctg actgcggtcc gcttcccgga
     1141 gttgtgcacc tccgacgtcc tcgcctaccc cgacctcctc cgtcgggccc tggccagcgg
     1201 ggcctgggcc caggtcccgt cggctgccgt cgccgaggag gtgctcgagc acttcgccgt
     1261 cccggccgac cacgtcgtgg tggtggccaa cggggtcgac gacgtgccga ccgctccaga
     1321 aggcgccggc cgggcccggg ccgggcgcga gcgctacgtc ctcgctctgg ggaccttcga
     1381 gccccgcaag gacctgccca cgctcgtggc cgccttcgac gccgcggcga acgacgatcc
     1441 cgacgtcgga ctggtcctcg ccggtgcgga cgggtggggc gccgaggcgg tacacaccgc
     1501 tgtagccaac tccccgcacc gggaacggat ccgcatcacc ggatggctcg acgaccaggc
     1561 ccgggccgcg ctcctgcggg aggcgaccgt cctggcctac ccgtcgctgt atgaggggtt
     1621 cgggctccct cccctggagg cgatgggggt cggggtcccg gtcgtggcca cccgggtcgg
     1681 cgccctgccc gaggtcctcg gcgacgccgc cgacctcgtt cccccgagcg acgtcgacgc
     1741 cctggcccaa gccctggtgc gggttctcac cgacgaggcg caccgggccg ccatggtggt
     1801 ccgaggccac cgtcgagcgg atcgctactc gtgggaggcc catgccgacg gcctggtggc
     1861 cctgtaccgc cgagcggcgg cgagtgcccc gggcgccgct ccgtagactc cggccggtgc
     1921 gagccctggt caccggcgca gccggcttcg tcgggcgcca cctcgtcgcc gggctgcagg
     1981 aggccggcga cgacgtccac gagaccgacc gtcatctcgg cctcgacatc ctggacccgg
     2041 cggggctgac cgacctgttc acgtcggtcc gcccggaggt cgtgtaccac ctcgccgggc
     2101 aggccgatgt gggagggtcg tggaccagcc cggtcgagac cttccgcacc aacgccgagg
     2161 gcaccctgaa cgtgctgtac gccgccgtgc aggccggtgt ggagcgggtc gtggccgtgg
     2221 ccagcgccga tgtgtacggc caggtgcccg aggccgacct gccgatcggt gaggatcggg
     2281 cctttcgccc cgtcagtccc tacggcgcca gcaaggcggc cgcggatgtg atcgccctgc
     2341 aggcgtacct cggccacggc ctggccgtgc tccgggcccg gcccttcaac cacctcgggc
     2401 ccgggcagag cgaccgcttc gtgacgtccg cggtggcctc ccgcatcgcc cgcaacgagg
     2461 tcagcggcag cgacgaggta ccggtcggca acctgagcgc ccgccgggac ttcaccgacg
     2521 tgcgggacgt ggtccgggcc taccggctcc tggctgagcg aggccagccc ggcgaggcct
     2581 acaacgtgtg ctccggtcgc gacatcgccg tccaggaggt cgccgaccgc ctcatcgccc
     2641 tggccgagcg cccgatggcc ttggtcccgg atcccgacct gctccgcccg gtggacacgc
     2701 cggtcctgcg gggcgatccc acccgcctcc acgaggcgac cggatgggaa cccacgatct
     2761 ccctcgacca gaccctggcc gacgtcctcg acgactggcg caaacgcgtg cgcgagacct
     2821 gagcccgcgg tcccctcagc cggccgagcg ctcgtccagg gcccgccgga ccttgccgcg
     2881 acacccctcg accaggtcgc ggtcgtgacg gtcgcattcc tgggccagcc gccgcagggc
     2941 cacgtcccac tgcgcgagcc gctggtcgcc gtagagcgac gggccccggc tgatccaggc
     3001 caggtcggtc tcacagaggg ccacctgctg ctcggcctcg gtgtaccggt tggccacgac
     3061 cggcaggcga tcggccttgg ccgtcggata ggcggttccc gccacgagtt cgtgcggctc
     3121 gagcttgaac aggccggcca gcagggtcac ggtgcgctca ccggggtacg acatgcctgc
     3181 ctcgacgtgg gagatggtga cccgcgagac gccgagacgc tccgccaggt cggcctgcgt
     3241 ccaaccctgc ttggcgcggt ggtccgcgat gcggcggccc agcacgttgc gctccagttc
     3301 gcgggccatg gggtcaaggt acgggaacgc ggggtcggcc cgctgccaag ccagttggca
     3361 gacacggtcc ctcccccggg atacaacggg gcccatgccc cgacgcgcgc tcatcaccgg
     3421 catcaccggc caggacggtt cctacctggc cgagcacctc ctcgaccacg gctacgaggt
     3481 ggtcggcatg gtccggcgat ccagcaccgt gaacttcgag cggatcgccc acatccagga
     3541 ccgcatcacc atcgtgcccg gtgacgtgct cgacgagatc tccatgatcg agatcctctc
     3601 caccca
//
LOCUS       DASTFV010000659         3081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2481569, whole genome shotgun sequence.
ACCESSION   DASTFV010000659 DASTFV010000000
VERSION     DASTFV010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2481569"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VFP02_12110"
     CDS             <1..1053
                     /locus_tag="VFP02_12110"
                     /inference="COORDINATES: protein motif:HMM:NF037725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HET9543825.1"
                     /translation="PMYGADPRFFPLGTKTGCRRLFAEEAVRHPIGFEDLHTVDDVVA
                     AFARLRAARPEVAHALVKLNEGVSGEGNAVVDLQRLPTPGAPGECEEVAERVRQMAFE
                     APDMPYDAYMAKLEEHGGIVEELISGVELRSPSVQLRVAPTGEVELLSTHDQLLGGPS
                     GQSYLGCCFPADFAYAKAISVDARTIGERLAKEGVVGRFAIDFVVVRSTDGGWTPYAI
                     ELNLRKGGTTHPFLTLQFLTDGRYDPMTALFLTPMGKEKHLIATDHLESDSLRALSLD
                     DLFDVVARHGLHFDQARETGVVFHMISCLTEHGRVGLTAVGNTPAEAESLYQRAERIL
                     LDEAALALAELQLPED"
     gene            1189..2037
                     /locus_tag="VFP02_12115"
     CDS             1189..2037
                     /locus_tag="VFP02_12115"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9543826.1"
                     /translation="MSELTTHETEQVAEVNASGKTPVMFVHGLWLLPNSWDRWRTVFE
                     EAGFATLAPGWPDDPETVEEAKAHPEVFAHKGVGDIADHYEAVTAELSTKPVVVGHSF
                     GGLLTQILAGRGRSAVAVAIDPAPFRGVLPLPISALRSASPVLGNPANRGRAVPLTLE
                     QFRYGFANEVSEEEAKELYETFAVPGSGEPIFQAAAANFNPWSETKVRSKNPERGPLL
                     IISGEKDHTVPPAIANASYKKQKRNAGVTEIVEMPGRGHSLVIDNGWREVADTALGFV
                     QRFASP"
     gene            2074..2439
                     /locus_tag="VFP02_12120"
     CDS             2074..2439
                     /locus_tag="VFP02_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9543827.1"
                     /translation="MQLTGYQELSPDELDGTFAALADPTRRAILARLAGGEATVTELA
                     RPFAMSQPAISKHLKVLERAGLVSRRRDAQRRPCRLEAARLKAATDWLAAYREHWEGS
                     FQRLDALLDELQTQGEDPS"
     gene            2436..>3081
                     /locus_tag="VFP02_12125"
     CDS             2436..>3081
                     /locus_tag="VFP02_12125"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9543828.1"
                     /translation="MTDFATLEVSTPSDTEIVMTRSFAAPRQVVFDAWTRPELLKRWY
                     NPLPWQLVECDIDLRVGGAWRFRSEGPDGASMVQGGVYRVLEPPERLVSTEWMNEEPE
                     DEADSVVNTFVLTEDAGRTTLTITMACPSREVRDMIAATGMAEGVEGSYQRLDALVDT
                     AALADRYRSVAADFTAIVEDAPADGWSNPSPCEGWAARDVVRHMVDMHSFLLGMI"
BASE COUNT          494 a         1083 c         1039 g          465 t
ORIGIN      
        1 ccgatgtatg gcgccgaccc caggttcttc cccctcggga ccaagaccgg atgccgccga
       61 ttgttcgccg aagaggctgt gcgtcatccg atcggtttcg aggacctgca caccgtcgac
      121 gacgtcgtcg cggccttcgc ccgcttgcgg gccgcccgtc ctgaggtcgc ccatgccctc
      181 gtgaagctca acgagggcgt gtctggagag gggaacgccg tcgtcgacct tcagcgcctt
      241 ccgacccccg gcgcgccggg cgagtgcgag gaggtcgcgg agcgggtccg gcagatggcg
      301 ttcgaggcgc cggacatgcc gtatgacgcg tacatggcca agctcgagga gcacggtggc
      361 atcgtcgaag aactgatcag cggcgtggag ctccgaagcc cgagtgtcca actgcgggtg
      421 gcaccgaccg gtgaggtcga gttgctgtcg acccacgacc agctgctcgg cggtccgagc
      481 gggcagagct atctcggctg ttgcttcccc gccgacttcg cctacgcgaa ggcgatcagc
      541 gtcgacgccc gaacgatcgg cgagcggctg gccaaggaag gcgtcgtcgg ccgattcgcc
      601 atcgatttcg tcgtggtccg gagcaccgac ggcgggtgga cgccatacgc catcgagctc
      661 aacctccgga agggcgggac cacgcacccg ttcctgaccc ttcagttctt gaccgatgga
      721 cgctatgacc cgatgaccgc gctcttcctc acgccgatgg gcaaggagaa gcacctgatc
      781 gccaccgatc acctcgagtc cgactcgctc cgggcactca gcctcgatga cctgttcgac
      841 gtcgtcgccc gccatggtct gcatttcgac caggcccgag agaccggggt ggtcttccac
      901 atgatcagct gcctgaccga acacgggcgt gtcgggctca ccgccgtggg caacacccct
      961 gctgaggccg agtccctcta tcaacgggcg gagcgaatcc tcctcgacga agcggctctc
     1021 gcattggccg agctgcaact gccggaggac tgaccactcg gcccgatcgg ccgccgacgg
     1081 cgcggacagc gaagtgacct ggtcgtaggg tgccagcgac agccatcgcg gcgggttcgg
     1141 gcggcacccg gacctccttc gtggcatccc caccccccag gagaccccat gtccgagctg
     1201 accacccacg agaccgagca ggtcgcggag gtaaacgcat ccgggaagac cccggtgatg
     1261 ttcgtccacg gcctgtggct cctgcccaac agctgggacc gctggcgcac cgtcttcgag
     1321 gaggcgggct ttgccacgct ggcgccgggt tggcctgacg atcccgagac ggtggaggag
     1381 gccaaggccc atcccgaggt gttcgcccac aagggcgtgg gcgacatcgc cgaccactac
     1441 gaggccgtca ccgccgagtt gagcaccaag cctgtggtcg tcggccattc cttcggcggc
     1501 ctgctgaccc agatcctcgc cggccggggc cggtcggccg tggccgtggc catcgacccc
     1561 gccccgttcc gaggcgtgct gcccctgccc atctcggccc tgcgctcggc gtcgccggtg
     1621 ctcggcaacc cggccaaccg gggccgggcc gtccccctga ccctcgagca gttccgctac
     1681 ggattcgcca acgaggtcag cgaggaggag gccaaggagc tgtacgagac cttcgccgtc
     1741 cccggatcgg gcgagccgat cttccaggcc gcggcggcca acttcaaccc gtggagtgag
     1801 acgaaggtcc ggtccaagaa cccggagcgt ggccctctgc tcatcatctc gggcgagaag
     1861 gaccacaccg tcccgccggc catcgccaac gcctcctaca agaagcagaa gcgcaacgcg
     1921 ggcgtgacgg agatcgtgga gatgcccggt cggggccact ccctggtcat cgacaacggt
     1981 tggcgagagg tggcggacac cgccctcggc ttcgtgcagc gcttcgcttc tccttgacag
     2041 caggcccggt gcactgtaga taactagtca gttatgcaac tgactggtta tcaagagctg
     2101 agcccagacg agctggacgg cacgttcgcg gccctcgccg accccacccg acgggccatc
     2161 ctggcccgac tggccggggg cgaggccacc gtgaccgagc tggcccggcc gttcgccatg
     2221 agccagcccg ccatctccaa gcacctgaag gtgctggagc gggccggcct ggtgtcgcgc
     2281 cggcgggacg cccagcggcg gccctgccgc ctggaggcgg cccgcctgaa ggcggccacc
     2341 gactggctgg cggcgtaccg ggagcactgg gaggggagct tccagcgcct cgacgccctg
     2401 ctcgacgaac tgcagaccca aggagaggat ccgtcatgac cgacttcgcc accctggagg
     2461 tgagcacccc gtcggacacc gagatcgtga tgacccggag cttcgccgcc ccccgccagg
     2521 tcgtcttcga cgcctggacc cggcccgagc tcctgaagcg ttggtacaac ccgctgccct
     2581 ggcagttggt cgagtgcgac atcgatctgc gggtcggcgg ggcctggcgc ttccgatcgg
     2641 agggtccgga cggggcgtcc atggtccagg gtggggtcta ccgggtgctc gagccgcccg
     2701 aacgcctcgt gtccaccgag tggatgaacg aggagccgga ggacgaggcc gactcggtgg
     2761 tgaacacctt cgtcctcacc gaggacgccg gtcggaccac cctcacgatc acgatggcct
     2821 gcccgtcccg cgaggtccgg gacatgatcg ccgccaccgg gatggccgag ggggtcgagg
     2881 gcagctatca gcgcctggac gcgctggtcg acaccgcggc gctggccgac cgctaccgct
     2941 cggtggcggc tgacttcacc gccatcgtcg aggacgcgcc ggccgatggc tggtcgaacc
     3001 cttcaccctg tgaggggtgg gcggcccggg acgtcgtccg ccacatggtg gacatgcaca
     3061 gcttcctgct cggcatgatc g
//
LOCUS       DASTFV010000660         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2489526, whole genome shotgun sequence.
ACCESSION   DASTFV010000660 DASTFV010000000
VERSION     DASTFV010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2489526"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /gene="mreB"
                     /locus_tag="VFP02_12130"
     CDS             <1..949
                     /gene="mreB"
                     /locus_tag="VFP02_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HET9543829.1"
                     /translation="YAKGRGIVLNEPSVIALNRQTGEVLATGYDAWKMIGRTPGYIVA
                     VRPLRGGAITDFEITERLIRMLFSQAGVSRFYRTRVLICVPSAITHVERRAVTEAARR
                     AGAAECQLIEQPMAAAIGAELPINEPIGSMVVDIGGGTSESAILSLGGIVSLKAVRVG
                     SFDFDAAIQTHIRKEHGIAIGEQTAEKIKIAMGSAYPTSEEFRAEVRGRELMSGLPKT
                     VVLEPEEIRGALEEPITTIIDSIVACLGEAPPDLAQDLIVNGIHLVGGGGLLRGLKDR
                     LSLETEVHVKLVDLPLEVVVLGAGRCIESYDALRVMFME"
     gene            1026..1796
                     /locus_tag="VFP02_12135"
     CDS             1026..1796
                     /locus_tag="VFP02_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9543830.1"
                     /translation="MGTLLGVQDVSVRFGGVIALNGLTFTVDEGQMCALIGPNGAGKT
                     TLFNVISRLYEPTGGSVTFAGDDLLAVPAHRIAKIGIARTFQNVALFPGLSVRDNVLM
                     GAFSDTKAGFVSTTVRWPTAIKEERASRVTADALLDELKLGAVAHRPAAGLPYGTLKR
                     VELARALASNPRMLMLDEPATGLTHGEVGELADLVRQVRDQRNLTVLLVEHHMEMVMR
                     ISEKIVVLDYGAKIAEGDPDVVRNDPRVIEAYLGGGDS"
     gene            1793..>2443
                     /locus_tag="VFP02_12140"
     CDS             1793..>2443
                     /locus_tag="VFP02_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9543831.1"
                     /translation="MTALLEVTDLEAGYGPVQVLRGMSFTVDAGETVVILGANGAGKT
                     TTIRAISGMIVTKGSVIVDGTSVGGKKPEEIVRRGVAHVPQGRGTFVDLTVEENLRLG
                     AFTRKDADIVADMDHWYEVFPRLQERRDQVAGGMSGGEQQMLAIARAAMSRPRLLLLD
                     EPSLGLAPLVTQELFRRLQVLNQEQGTALLVVEQNANLALGIAERGYVLEAGLIVAQ"
BASE COUNT          379 a          864 c          844 g          356 t
ORIGIN      
        1 ctacgccaag ggcaggggca tcgtcctgaa cgagccgtcg gtcatcgcgc tcaaccggca
       61 gacgggggag gtgctggcca ccgggtacga cgcctggaag atgatcggac gcacacccgg
      121 ctacatcgtc gccgtgcggc cgctgcgcgg cggagcgatc accgacttcg agatcacgga
      181 acggctcatc cggatgctgt tctcccaggc cggcgtgtca cgcttctatc gaacccgtgt
      241 gctgatctgc gtgccgtcgg cgatcaccca cgtcgagcgc cgcgccgtca ccgaagccgc
      301 tcgccgcgcc ggcgccgccg agtgccagct catcgagcag cccatggcgg ccgccatcgg
      361 ggccgaactg cccatcaacg agccgatcgg ctccatggtc gtcgacatcg gcggcgggac
      421 ctccgagtcg gccatcctct ccctgggcgg catcgtctcc ctcaaggccg tgcgggtggg
      481 cagcttcgac ttcgacgccg ccatccagac ccacatccgc aaggagcacg gcatcgccat
      541 cggcgagcag acggcggaga agatcaagat cgcgatgggc tcggcctacc ccacctccga
      601 ggagttccgg gccgaggtcc ggggtcggga gctgatgtcg ggcctgccca agacggtggt
      661 gctggagccc gaggagatcc ggggcgccct cgaggagccc atcaccacga tcatcgactc
      721 catcgtggcc tgcctgggcg aggccccgcc cgatctggcc caggacctca tcgtcaacgg
      781 catccacctg gtcgggggtg gcggcctgct gcggggccta aaggaccgct tgagcctgga
      841 gaccgaggtc cacgtcaagc tcgtcgacct ccccctggag gtcgtcgtgc tcggcgccgg
      901 gcgctgcatc gagtcctacg acgccctgcg cgtcatgttc atggaatagc gggccacggg
      961 ttccggccga ccgatcggcg gcggagccgc gccggcggcg acgggcaagt actgtcccga
     1021 gccgaatggg gacgctgttg ggggtgcagg acgtcagcgt gcgcttcggc ggcgtcatcg
     1081 cgctgaacgg cctcaccttc accgtcgacg agggccagat gtgcgccctg atcggcccga
     1141 acggggcggg caagacgacc ctgttcaacg tcatcagccg gctctacgaa cccaccggcg
     1201 gttcggtcac ctttgccggc gacgacctct tggccgtgcc cgcccaccgc atcgccaaga
     1261 tcggcatcgc ccgcaccttc cagaacgtgg ccctctttcc cggcctgtcc gtgcgggaca
     1321 acgtcctgat gggggccttc tccgacacca aggcgggctt cgtgtccacg acggtgcgtt
     1381 ggcccaccgc catcaaggaa gagcgcgcca gccgggtgac cgccgacgcc ctgctcgacg
     1441 aactgaagct gggcgcggtc gcccaccgac cggccgccgg cctgccctac gggacgctga
     1501 agcgggtcga gctggcccgg gccctcgcct ccaacccccg gatgctcatg ctcgacgagc
     1561 cggcgaccgg cctgacccac ggtgaggtgg gcgagctggc cgacctggtc cgtcaggtgc
     1621 gcgaccagcg gaacctgacc gtcctgctcg tcgagcacca catggagatg gtcatgcgca
     1681 tctccgagaa gatcgtggtc ctcgactacg gcgccaagat cgccgagggc gatccggacg
     1741 tcgtccgcaa cgatccccgg gtcatcgagg cctacctggg cgggggcgac tcgtgaccgc
     1801 gctgctggag gtcaccgacc tcgaggccgg ctacggcccg gtccaggtcc tgcgggggat
     1861 gtccttcacc gtcgacgcgg gcgagacggt ggtcatcctc ggggccaacg gggcgggcaa
     1921 gacgacgacc atccgcgcca tcagcgggat gatcgtcacc aaggggtcgg tcatcgtcga
     1981 cggcacgtcg gtgggcggca agaagccgga ggagatcgtc cgacggggcg tggcccatgt
     2041 tccccagggg cggggcacct tcgtggacct cacggtggag gagaacctcc gcctcggcgc
     2101 cttcacccgc aaggacgccg acatcgtcgc cgacatggac cactggtacg aggtcttccc
     2161 ccgcctgcag gagcgccgcg accaggtcgc cggcgggatg agcgggggcg agcagcagat
     2221 gctggcgatc gcccgggcgg ccatgtcccg gcctcgcctc ctgctgttgg acgagccgtc
     2281 gctgggcctg gccccgctcg tgacccagga gctgttccga cggctgcagg tgttgaacca
     2341 ggaacagggg accgccctgc tcgtcgtgga gcagaacgcc aacctggccc tcggcatcgc
     2401 cgagcggggc tatgtgctcg aggccggcct gatcgtggcc cag
//
LOCUS       DASTFV010000661         2543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2492498, whole genome shotgun sequence.
ACCESSION   DASTFV010000661 DASTFV010000000
VERSION     DASTFV010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2492498"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFP02_12145"
     CDS             <1..204
                     /locus_tag="VFP02_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543832.1"
                     /translation="TSGRDLAEIDYYIAFGFWKLACIGEGVLKRYQAGVMGGPDASAL
                     VEMFTHHVANLANAAATTLGMDP"
     gene            201..1073
                     /locus_tag="VFP02_12150"
     CDS             201..1073
                     /locus_tag="VFP02_12150"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HET9543833.1"
                     /translation="MSHPLYEFHQDPGLSEPVLLVALEGWIDAGGGAASAAETILASA
                     EQIPVATFNSDTLLDHRARRPIMHLVDGVNTGLTWPTIRLDETWDREGRDVLFLHGPE
                     PDHNWPSFAKAVIELAEGFEVTEVYGLGAYPAPVPHTRPVQLASTASSHDMARRVGFI
                     RGTLEVPAGVAAAIEAQAAEIGMSALGLWAQVPHYAAAMPYPAAAVRLIDGLFEVAGL
                     RFDHGELDAEVETIRDRLDELVANSDENQELVHQLEIHVDQSEPDPEDGGIELRSGDE
                     LAEEFERFLREQDD"
     gene            complement(1077..>2543)
                     /locus_tag="VFP02_12155"
     CDS             complement(1077..>2543)
                     /locus_tag="VFP02_12155"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9543834.1"
                     /translation="PDTCDTERGTVAIPNYFAAECYAPFEGDNGGATDTGVTDDTITI
                     AFYQSPPNPISDFILGGFGITDTNEEALATMQTYVDIFNEYFETYGRTVEVVEVEGSG
                     PPNDDVAARADAVRVDEEIGAFMVWGGPILTDAFADELAARQIPCISCQVGEPDAFAA
                     DRSPYVITVANSPDQGRAHLVEWLSKQVAGRPAEFAGEDLQSEDRVFGTLFLESDEDA
                     AAENQVTIDMLADEGIDVAERIAYQLDPARLQEQAASAIARFKEAGVTSIIFSGDPVA
                     PATFTTEATNQDYFPEWIITGSALTDTTAAARSFDQEQWSHAFGISSLAARLNPENSG
                     SFYLYTWYTGEEPPAADTTGVILPLPQVFFGALQEVGPELTREAWVAALLAAEPTPDA
                     ISQPSLDWGDVEGWPATDYHGIDDWTEIWWDSESEGPSEIRRVEPGIYQYVDGGRRYR
                     LGEWPEEDSRAFDPEGAVAFYEQPPEGEEAPDYPSPAG"
BASE COUNT          364 a          895 c          893 g          391 t
ORIGIN      
        1 acctccggcc gggacctggc cgagatcgac tactacatcg ccttcggctt ctggaagctg
       61 gcctgcatcg gggagggcgt gctcaagcgc taccaggccg gcgtcatggg tggccccgac
      121 gccagcgcgc tggtcgagat gttcacccac cacgtggcca acctggccaa cgcggcggcc
      181 accaccctgg ggatggaccc gtgagccacc cgctctacga gttccaccag gacccggggc
      241 tgtccgagcc cgtcctgttg gtggccctgg agggctggat cgacgccggc ggcggtgccg
      301 cctcggcggc ggagaccatc ctggcctcgg ccgaacagat cccggtggcc accttcaaca
      361 gcgacaccct cctcgaccac cgggctcggc gcccgatcat gcacctggtc gacggggtga
      421 acacgggtct cacctggccg accattcgcc tggacgagac ctgggaccgc gaggggcggg
      481 acgtgctctt cctccacggg ccggagcccg accacaactg gccgtcgttc gccaaggccg
      541 tcatcgagct cgccgagggc ttcgaggtca ccgaggtcta cgggctcggg gcctaccccg
      601 cccccgtccc ccacacccgc cccgtacagc tggcctccac ggcgtcctcc cacgacatgg
      661 cccggcgggt cggcttcatc cggggcaccc tcgaggtccc cgccggggtg gcagcggcca
      721 tcgaggcgca ggcggccgag atcggcatgt ccgccctcgg cctctgggcc caggtccccc
      781 actacgccgc ggccatgccc tatcccgccg cggccgttcg cctcatcgac gggctcttcg
      841 aggtcgccgg gttgcgcttc gaccatggcg agctggatgc ggaggtggag acgatccggg
      901 accgcctcga cgagctcgtg gccaacagcg acgagaacca ggaactggtc caccagttgg
      961 agatccacgt cgaccagagc gagcccgacc ccgaggacgg cgggatcgag ctgcgctccg
     1021 gtgacgagct ggcggaggag ttcgagcggt tcctgcgcga gcaggacgac tagcgctcag
     1081 cccgcgggcg acgggtagtc cggggcctcc tcgccctccg gaggctgctc atagaaggcc
     1141 accgcgccct cgggatcgaa ggcccggctg tcctcctcgg gccactcccc gaggcggtag
     1201 cggcggccgc cgtccacgta ttggtagatg ccgggctcga cccggcggat ctcactcggc
     1261 ccctcggact ccgagtccca ccagatctcg gtccagtcgt cgatgccgtg gtagtcggtc
     1321 gcgggccagc cctcgacgtc accccagtcc agcgagggct ggctgatggc atcgggcgtg
     1381 ggctcggccg ccaggagggc cgccacccag gcctcgcgcg tcagctccgg cccgacctcc
     1441 tggagcgcac cgaagaagac ctgagggagg ggcaggatca ccccggtcgt gtccgcggcc
     1501 ggaggctcct cgcccgtgta ccaggtgtag aggtagaagg atccggagtt ctcggggttc
     1561 agccgggccg cgaggctgct gatgccgaac gcgtgcgacc actgctcctg gtcgaagctg
     1621 cgggccgccg ccgtcgtgtc ggtgagggcc gagccggtga tgatccattc cgggaagtag
     1681 tcctggttcg tggcctcggt cgtgaacgtc gccggcgcca ccggatcgcc ggagaagatg
     1741 atgctggtca ccccggcctc cttgaagcgg gcaatggcgc tggccgcctg ttcctgcagc
     1801 cgggcgggat cgagctggta ggcgatgcgc tccgccacgt cgatgccctc gtcggccagc
     1861 atgtcgatcg tgacctggtt ctcggcggcc gcgtcctcgt cactctccag gaagagggtg
     1921 ccgaacacgc ggtcctcgct ctgcaggtcc tcaccggcga actcggccgg ccggccggcg
     1981 acctgcttgg agagccattc cacgaggtgg gcccgaccct gatcggggga gttggcgacc
     2041 gtgatgacgt acggggaccg gtcggccgcg aaggcgtcgg gctcaccgac ctggcagctg
     2101 atgcacggga tctggcgggc cgccagctcg tcggcgaagg cgtcggtcag gatcgggcca
     2161 ccccagacca tgaaggcccc gatctcctcg tcgacccgca cggcgtcggc ccgggcggcc
     2221 acgtcgtcgt tcggtgggcc cgagccctcg acctcgacga cctcgaccgt gcggccgtag
     2281 gtctcgaagt actcgttgaa gatgtcgacg taggtctgca tggtcgccaa cgcctcctcg
     2341 ttggtgtcgg tgatgccgaa gcccccgagg atgaagtccg agatcgggtt gggcggtgac
     2401 tggtagaagg cgatggtgat cgtgtcgtcg gtcacccccg tgtcggtggc cccaccgttg
     2461 tccccctcga agggggcgta gcactcagcc gcgaagtagt tcgggatggc gacggtgccg
     2521 cgctcggtgt cacaggtgtc ggg
//
LOCUS       DASTFV010000662         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2496335, whole genome shotgun sequence.
ACCESSION   DASTFV010000662 DASTFV010000000
VERSION     DASTFV010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2496335"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFP02_12160"
     CDS             <1..358
                     /locus_tag="VFP02_12160"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET9543835.1"
                     /translation="RGPMVIVGDAAHAPSPTSGQGASMAAEDAVVLAQCLRDLPTVGD
                     ALAAYEGLRRRRVERIVAHGARTGRSKTPGPVGRVVRDLALPLVFKLLVTERSMAWIY
                     DHHIDWDTPVRGPQRG"
     gene            complement(336..1676)
                     /locus_tag="VFP02_12165"
     CDS             complement(336..1676)
                     /locus_tag="VFP02_12165"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HET9543836.1"
                     /translation="MKLIGRRRVIWDYLAGALWVLPTLSVAVFLLAGAALSRVEVDGD
                     SPLARFAFQGTAEDARSLLVVVSSTMITVTGLVFALTIVALQIASGQYSPRLLRNFMR
                     DRGTQLVLSIFVGAFAYSTAGLYTVGIQSTGQEAFVPRLAVSGSLALALASVGVLIYF
                     IHHLAHSIQIDTIMSQVEREALSVIDDVYPHQPRYREPEERCPEPPLEAVGLPSARSG
                     YVQAIHPESLVSAAVRDDLVIVLARQVGDHIVAGTPIAWAWRRSPGQPPPGADALRQA
                     VADAVQLGFERTMVQDVGFGIRRLVDIANKALSPAINDPYTGVQAVHHLSVLLCRLAR
                     LRLGDWTVHDEQGELRVAVPRSKFADYLRLGTAQIRRFGAGEPAVTRALIVLLRDAGT
                     SSATEDRRHACVRNIRLVLDDARRATAQPADAEAVATEAATALRLLGFNPSEVP"
     gene            complement(1673..2449)
                     /locus_tag="VFP02_12170"
     CDS             complement(1673..2449)
                     /locus_tag="VFP02_12170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9543837.1"
                     /translation="MTEQDEDDDQPRLYTELADWFHLLTAPADYAEDARLYLDLLTDA
                     GGAPPRTLLELGSGGGNNASHLKAHFELTLVDLSAGMLEVSREANPECEHVQGDMRTV
                     RLGREFDAVFVHDAVMYMTTAEDLRLAIETAFVHCRPGGAALFAPDHLREHFRTGTDY
                     GGHDGDQRAVRFLEWTWDPDPLDTTYTVDYAYLLRDTDGSVRVEHDRHVEGLFPRATW
                     LELLADAGFEARAVPVEHSELEPGSYELFVATRPNESAPG"
BASE COUNT          356 a          895 c          960 g          337 t
ORIGIN      
        1 ccgcggcccg atggtgatcg tcggcgacgc cgcccacgcc ccctcgccca cctcgggcca
       61 gggcgcctcc atggccgccg aggacgccgt cgtgctcgcc cagtgcctcc gcgacctccc
      121 caccgtcggt gacgccctgg ccgcctacga gggcctgcgc cgccggcggg tcgagcgcat
      181 cgtcgcccac ggcgcccgga cgggcaggtc caagaccccc ggcccggtcg gccgggtcgt
      241 gcgcgacctg gccctccccc tggtcttcaa gctgctcgtc accgagcgct cgatggcctg
      301 gatctacgac caccacatcg actgggacac ccccgtcagg ggacctcaga ggggttgaac
      361 ccgagcaggc gcagggcggt ggcggcctcg gtggccaccg cctcggcgtc ggcgggctgg
      421 gcggtggcgc ggcgggcgtc gtcaaggacc aggcggatgt tgcggacgca ggcgtggcgg
      481 cggtcctcgg tggccgagct ggtgccggcg tcgcggagca ggacgatcag ggcgcgggtc
      541 acggccggct cgcccgcccc gaagcggcgg atctgggcgg tgcccaggcg caggtagtcg
      601 gcgaacttgg agcggggaac ggccacccgc agctcccctt gctcgtcgtg gaccgtccag
      661 tcgcccagac gcaggcgggc caggcggcac agcagcaccg acagatggtg gaccgcctgg
      721 acgccggtgt aggggtcgtt gatggccggc gacagcgcct tgttggcgat gtcgaccagc
      781 cggcggatgc cgaagccgac gtcctggacc atggtccgct cgaagccgag ctggacggcg
      841 tcggccaccg cctggcgcaa ggcgtcggcc ccgggcggcg gctgccccgg cgaccggcgc
      901 caggcccagg cgatcggggt cccggccacg atgtggtcgc cgacctgcct ggccagcacg
      961 atcaccaggt cgtcgcggac ggccgccgag accagcgact cggggtggat ggcctggacg
     1021 tacccggagc gagccgatgg caggccgacg gcctccagcg gcggctcggg gcatcgctcc
     1081 tccggctccc ggtagcgggg ctggtggggg tagacgtcgt cgatcaccga cagggcttcg
     1141 cgttcgacct ggctcatgat ggtgtcgatc tggatcgagt gggccagatg gtggatgaag
     1201 tagatgagca ccccgacgct ggccagggcc agcgccagcg accccgagac ggccagccgg
     1261 gggacgaacg cctcctggcc cgtgctctgg atccccacgg tgtacagccc ggccgtggag
     1321 taggcgaagg cgccgacgaa gatgctcagc acgagctggg tgccccggtc gcgcatgaag
     1381 ttgcgcagca gccgcggcga gtactggccg gaggcgatct ggagggcgac gatggtgagg
     1441 gcgaacacca ggccggtgac cgtgatcatg gtggacgaca ccacgaccag cagcgagcgg
     1501 gcgtcctcgg cggtgccctg gaaggcgaag cgcgccaggg gcgagtcccc gtccacctcc
     1561 acccgcgaca gggccgcccc cgccagcagg aacaccgcca ccgagagcgt ggggaggacc
     1621 cagagggcac cggccagata gtcccagatg acccggcgcc ggccgatcag cttcatcctg
     1681 gtgcggactc gttcggcctg gtggcgacga acagctcgta gctgcccggc tccagctccg
     1741 agtgctccac cgggaccgct ctggcctcga agccggcgtc ggccagcagc tccagccagg
     1801 tggcccgggg gaacagcccc tcgacgtggc ggtcgtgctc gacgcggacc gagccgtcgg
     1861 tgtcgcgcag caggtaggcg tagtcgacgg tgtaggtggt gtcgagcggg tcggggtccc
     1921 aggtccactc caggaaccgg acggcgcgct ggtcgccgtc gtggccgccg tagtcggtgc
     1981 cggtgcggaa gtgctcgcgc aggtggtcgg gggcgaacag ggccgccccg cccggccggc
     2041 agtggacgaa ggccgtctcg atcgccaggc ggaggtcctc ggcggtggtc atgtacatga
     2101 ccgcgtcgtg gacgaacacg gcgtcgaact cccggcccag gcggaccgtc cgcatgtccc
     2161 cctggacgtg ctcgcactcg gggttggcct cccggctgac ctcgagcatg cccgccgaca
     2221 ggtcgaccag ggtgagctcg aagtgggcct tgaggtggga ggcgttgttg cccccgccgg
     2281 agccgagctc gagcagggtg cgaggcgggg cgccgcccgc gtctgtgagc agatcaagat
     2341 agagccgcgc gtcctcggcg tagtctgccg gcgccgtcag caggtggaac cagtccgcga
     2401 gctcggtgta cagccggggc tggtcgtcgt cctcgtcttg ctcggtcacg cctgcccccg
     2461 tcgcgctcgc accggtcgca cttgtacccc ggacgctgct caccccctgc tcaccctcgc
     2521 ctgcactcac ggcgagatct gggccgga
//
LOCUS       DASTFV010000663         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2507682, whole genome shotgun sequence.
ACCESSION   DASTFV010000663 DASTFV010000000
VERSION     DASTFV010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2507682"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..639)
                     /gene="upp"
                     /locus_tag="VFP02_12175"
     CDS             complement(16..639)
                     /gene="upp"
                     /locus_tag="VFP02_12175"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET9543838.1"
                     /translation="METTVVDHPLAAERLTTMRDEDTPRPVFRRALSELSWLLVYEAT
                     RDLQVTTFPVQTPLAPATGTRIDPVPLIVPVLRAGLGMLDAAFTMLPNAEVGFVGLRR
                     DEVTHDSKEYMTTVPDDLDGRPVIILDPMLATGGSLVHTVEIIERSNPGPIIVVCVLA
                     APEGIAHFAERCPDARVITASVDERLNDVAFIVPGLGDAGDRQFGVQ"
     gene            681..1625
                     /gene="deoC"
                     /locus_tag="VFP02_12180"
     CDS             681..1625
                     /gene="deoC"
                     /locus_tag="VFP02_12180"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET9543839.1"
                     /translation="MADTDLAPVERHSIPPVGGVSVRVTPVDAVGLEARAASLAGRSI
                     KRETKAAALDLAIRCCDLTTLEGSDTPGKVRALCAKAVHPDPTDRSVPSVAAVCVYPT
                     MVPVAADALAGTGVLVASVAGAFPSGLGPQETRVDEIRRAVAAGADEIDMVLNRSAFL
                     SGDFATTFDDIVASKEASGRAHLKVILETGELGSYDQVRRASMLAMAAGADVIKTSTG
                     KIGAAATPPVALCMAEAIRDFHEQTGRSVGLKLAGGIRSAKQSWQYLVIVGETLGPEW
                     LRPERFRLGASSLLNDLLMQRRWLATGRYQRPDDFTID"
     gene            1633..>2499
                     /locus_tag="VFP02_12185"
     CDS             1633..>2499
                     /locus_tag="VFP02_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9543840.1"
                     /translation="MTDLTQPGAFTLEYATAPESTAIVSLRDTYGLFIGGAWVEPRSG
                     DHFPTINPATEEELAMVAEAGAEDVDLAVAAARQAWEAGWRDLPGRERAKYLYRIARL
                     LQERSREFAVLETLDGGKPIKESRDVDLPLAAAHFFHYAGWADKLDYAFAGRRGRPLG
                     VAGQVIPWNFPMLMASWKLAPALATGNTVVLKPAETTPLTALLLAEVLQDAELPPGVV
                     NIITGAGATGSALVGHPGIDKVAFTGSTDVGKRIQTELAGTGKRLTLELGGKAANIVF
                     EDAALDQAVEGIV"
BASE COUNT          358 a          902 c          895 g          344 t
ORIGIN      
        1 actgacccga gaggcctact gcaccccgaa ctggcggtcg ccggcatcgc cgaggccagg
       61 gacgatgaac gcgacgtcgt tgaggcgctc gtccacgctg gcggtgatca cccgggcatc
      121 ggggcagcgc tcggcgaagt gggcgatgcc ctcgggggcg gccagcacgc agaccacgat
      181 gatcgggccg gggttcgagc gctcgatgat ctcgacggtg tgcaccagcg agccgccggt
      241 ggccagcatc gggtcgagga tgatgaccgg cctcccgtcg aggtcgtcgg gcaccgtggt
      301 catgtactcc ttggagtcgt gggtcacctc gtcgcggcgg aggccgacga aaccgacctc
      361 ggcgttcggc agcatcgtga aggcggcgtc gagcatcccc aggccggccc ggaggacggg
      421 cacgatcagg ggcacggggt cgatgcgggt cccggtggcc ggcgccagcg gcgtctgcac
      481 cgggaacgtg gtgacctgca gatcgcgggt cgcctcgtag accagcagcc acgacagctc
      541 cgacagggcc cgccggaaca cggggcgggg ggtgtcctcg tcccgcatcg tggtcagccg
      601 ttcggcggcg agggggtggt cgaccacggt ggtttccatg gcccgaatca tggctcagga
      661 ggcgctggta cggtccaaac gtggccgaca ccgacctggc gcccgtcgag cgccattcca
      721 taccacccgt gggcggggtg agcgtgcggg tgaccccggt cgacgccgtg ggcctcgagg
      781 cccgggctgc gtccctggcg ggccggtcga tcaagcgaga gaccaaggcc gccgctctcg
      841 acctcgccat ccgttgttgt gacctcacca cgctggaggg gtccgacaca ccgggcaagg
      901 tccgggccct gtgcgccaag gccgtgcacc ccgacccgac cgaccggtcg gtcccctcgg
      961 tggccgcggt ctgcgtctac cccaccatgg tgccggtggc ggccgacgcc ctggccggca
     1021 ccggcgtgct cgtggccagc gtggccggtg cgttcccgtc cggcctcggt ccccaggaga
     1081 cccgcgtcga cgagatccgc cgggccgtcg ctgcgggggc cgacgagatc gacatggtgt
     1141 tgaaccggtc ggccttcctg tccggtgact tcgccaccac cttcgacgac atcgtcgcct
     1201 ccaaggaggc ctcgggccgg gcccacctca aggtcatcct cgagaccggg gagctgggca
     1261 gctacgacca ggtgcgccgg gcgtcgatgc tggccatggc cgcgggggcc gacgtcatca
     1321 agacctccac cggcaagatc ggcgcggcgg cgacgccacc ggtcgccctg tgcatggccg
     1381 aggccatccg ggacttccac gagcagaccg gccggtccgt cggcctgaag ctggccgggg
     1441 gcatccgctc ggccaagcag tcctggcagt acctggtgat cgtcggagag accctcggtc
     1501 ccgagtggct gcgcccggag cgcttccgcc tcggggcctc cagcctgctc aacgacctgc
     1561 tgatgcagcg acgctggctg gccaccggcc gctaccagcg gcccgacgac ttcacgatcg
     1621 actgaggctg ccgtgaccga cctgacccag cccggtgcgt tcaccctcga gtacgccacg
     1681 gcgcccgaat cgaccgccat cgtgtcgctg cgggacacct acggcctgtt catcggcggt
     1741 gcgtgggtgg agccccgctc gggcgatcac ttcccgacga tcaacccggc caccgaggag
     1801 gaactggcca tggtcgccga ggccggggcc gaggacgtcg acctcgccgt cgccgcggcc
     1861 cgccaggcct gggaggcggg ctggcgggac ctgcccggcc gggagcgagc caagtacctg
     1921 taccgcatcg cccgcctcct gcaggagcgg tcgcgggagt tcgccgtcct ggagaccctc
     1981 gacggaggca agccgatcaa ggagtcccgc gacgtcgacc tccctctcgc cgccgcgcac
     2041 ttcttccact acgcgggctg ggccgacaag ctcgactacg cctttgccgg ccgtcggggt
     2101 cggccgctcg gcgtcgccgg ccaggtcatc ccctggaact tcccgatgct catggccagc
     2161 tggaagctgg ccccggcgct ggccaccggc aacaccgtcg tgctcaagcc cgccgagacg
     2221 acgcccctga cggcgctcct gctggccgag gtcctccagg acgccgagct gccgccgggc
     2281 gtggtcaaca tcatcacggg cgccggggcg acggggtcgg ccctggtcgg gcacccgggg
     2341 atcgacaagg tcgccttcac cggctcgacc gacgtgggca agcggatcca gaccgagctg
     2401 gccggcaccg gcaagcgcct gaccctggag ctcggcggca aggcggccaa catcgtcttc
     2461 gaggacgccg ccctggacca agcggtggag ggcatcgtc
//
LOCUS       DASTFV010000664         3372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2516968, whole genome shotgun sequence.
ACCESSION   DASTFV010000664 DASTFV010000000
VERSION     DASTFV010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2516968"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..548)
                     /locus_tag="VFP02_12190"
     CDS             complement(102..548)
                     /locus_tag="VFP02_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543841.1"
                     /translation="MTDDRPNGPWYRRPRPLAALALLVLVLGFGVGLLLAAGDDGDQV
                     DTTDTTVTVTTAPSETTVPSTIASTTSVAGDVDLAACENGDQVSCDRLDDDQLQAFCD
                     DGNVDACQVLLARQGDGVPDGPNGEGNGNGNGNGNGNGNGKGDEGD"
     gene            complement(556..1461)
                     /locus_tag="VFP02_12195"
     CDS             complement(556..1461)
                     /locus_tag="VFP02_12195"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9543842.1"
                     /translation="MAASDLVPPRDEGTVIVAADRRLGVAEFGPRDGKPVFWFHGTPG
                     ARCQVPPAARRGADELGARVIGVERPGTGSSTGHLYPDIRAFADDIAVVADELGVDRF
                     GVIGLSGGGPYVLACAEAFPDRVVGAVVLGGVAPTVGDDASGGGPVDLTRRLRLLIGV
                     TRLPLALVLTGLARALKPLGHQGLALYSRHSPPGDRIAFAAPGMEEMFLDDLNGAVAR
                     GGLGAATADLALFGCHWGFSVRDIRVPVRFWHGDADHIVPLHHVETLARLVPDAELHV
                     RPGESHLGTLVVGDEALRTVVDLWN"
     gene            complement(1475..1939)
                     /locus_tag="VFP02_12200"
     CDS             complement(1475..1939)
                     /locus_tag="VFP02_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9543843.1"
                     /translation="MSHPRAEIEAAVERYLQIRSDAEEGWGGWDGLDEMFTDDATFID
                     PAWGRVTGIENLREFWRESMAGLDDWRFPHGWSSIDGDQVVLMWWNRLPGQRADGTWY
                     EVPGVSLMTYAGDGKFSYEEDIMNMAHLFEVITESGYQFAENMNQPPRHPPR"
     gene            complement(1936..2721)
                     /locus_tag="VFP02_12205"
     CDS             complement(1936..2721)
                     /locus_tag="VFP02_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HET9543844.1"
                     /translation="MSTPAGWYPDPTNRHQNRWFDGNDWTDHVADGQAVSTDPVQGGP
                     AAASTQPTPAATPDPAATSVSPAASGPPPGAPGSPPPGGGAPLGTYAGPESSSSGGSG
                     KALLIAGVGAVLVAIVVALVLTLGGGDDDGGDAADVTVSDEDTTPATDDPSASDLFDD
                     MADTGNDGGDIFGTDDEPADDPADSTVPPDDSGDAAYPQEVIDNFTDSCESAGSSSTF
                     CDCVIDALQQDVPYDRFVEIDQQLAEDPSNIPTELTDAAEGCQ"
     gene            complement(2791..>3372)
                     /locus_tag="VFP02_12210"
     CDS             complement(2791..>3372)
                     /locus_tag="VFP02_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378494.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HET9543845.1"
                     /translation="DSMMLWDPIRYDETCPSSDGAFAMVLADEATGDASPNPAWLHGS
                     SLRSEATMAAGRDHVNPQAGRDCAADLYQQAGITNPRQQIDLAEVYVPFSWYEPMWLE
                     NLGFAEEGEGWKLTESGATALDGDIPWNASGGVLSTNPIGASGMIRFGEAAMQVRGMC
                     GDHQVDGARTALGHAYGGGAQFFAMWIVSSAKP"
BASE COUNT          442 a         1217 c         1217 g          496 t
ORIGIN      
        1 cgccgagtcc ccccgccgag ctgccccggc ggtcctcgat gttctcgctc cgaccctgac
       61 ccggcttcca gcgcatgggc cctccctacc ccgaagggcg ctcagtcgcc ttcgtcgccc
      121 ttcccgttgc cgttcccgtt gccgttgccg ttgccgttcc cgttgccctc gccgttggga
      181 ccgtcgggca ccccgtcgcc ctggcgggcc agcaggacct ggcaggcgtc cacgttgccg
      241 tcgtcgcaga acgcctgcag ttgatcgtca tccagtcgat cacaggagac ctgatcgccg
      301 ttctcgcagg ccgcgaggtc cacgtcgccc gccaccgacg tcgtgctggc gatggtcgac
      361 gggacggtcg tctccgacgg cgcggtggtc acggtgaccg tggtgtcggt ggtgtcgacc
      421 tggtccccgt cgtcgcccgc ggccaggagc aggcccacgc cgaacccgag gacgagcacg
      481 agcaacgcca gagcggcgag cggccggggc cggcggtacc agggcccgtt cggtcggtcg
      541 tcggtcatgg tcggctcagt tccacaggtc gaccaccgta cggagcgctt cgtcgcccac
      601 aaccaaggtc ccgagatggc tctccccggg gcggacgtgc agctcggcgt cgggcacgag
      661 ccgggccagg gtctcgacgt ggtgcagggg cacgatgtgg tcggcgtcgc cgtgccagaa
      721 ccgcacgggc acccggatgt cccgcaccga gaagccccag tggcagccga agagggccag
      781 gtcggcggtg gccgccccga gcccgcccct cgccacggcg ccgttgaggt cgtccaggaa
      841 catctcctcc atgcccggtg cggcgaaggc gatcctgtcg cccggtggtg agtgccgcga
      901 gtagagggcg agaccctggt ggccgagggg cttcagggcc ctggccaggc ccgtgaggac
      961 cagcgccagc ggcaggcggg tcacgccgat caggagccgg aggcgtcggg tgaggtcgac
     1021 gggccctcca ccggaggcgt cgtcgccgac ggtcggggcg acgccgccga gcaccaccgc
     1081 cccgaccacc cggtcgggaa aggcctcggc gcaggccagg acgtagggcc cacccccgga
     1141 caacccgatc accccgaagc ggtcgacccc gagctcgtcg gccaccaccg cgatgtcgtc
     1201 ggcgaacgcc cggatgtcgg ggtagaggtg acccgtcgac gacccggtcc ccgggcgctc
     1261 gaccccgatg acccgggccc ccagctcgtc ggccccgcgt cgggccgcgg gcgggacctg
     1321 gcaccgggcg cccggcgtgc cgtggaacca gaagacgggc ttgccgtcgc ggggcccgaa
     1381 ctcggccacg cccaggcggc gatcggcggc gacgatgacc gtgccctcgt cacgaggcgg
     1441 caccaggtcc gacgcggcca tggcgatcgg gcgtctaccg gggcgggtgg cggggcggtt
     1501 ggttcatgtt ctcggcgaac tggtagccgc tctcggtgat cacctcgaag agatgggcca
     1561 tgttcatgat gtcctcctcg taggagaact tcccgtcccc tgcataggtc atgagggaca
     1621 cgcccggcac ctcgtaccag gtgccgtccg ctcgttgacc gggaagccgg ttccaccaca
     1681 tgaggaccac ctggtccccg tcgatcgagg accagccgtg ggggaagcgc cagtcgtcga
     1741 ggccggccat gctctcccgc cagaactccc gcaggttctc gatgcccgtg acccggcccc
     1801 acgcggggtc gatgaacgtg gcgtcgtcgg tgaacatctc gtcgagaccg tcccagcccc
     1861 cccatccctc ctcggcgtcg gaccggatct gcaggtagcg ctcgacagcc gcctcgatct
     1921 cggcccgggg atggctcact ggcacccctc cgcggcgtcg gtcaactccg tcgggatgtt
     1981 cgacgggtcc tcggccagct gctggtcgat ctcgacgaag cggtcgtacg gcacgtcctg
     2041 ctgcagggcg tcgatcacgc agtcgcaaaa ggtggacgag ctcccggcgc tctcgcacga
     2101 gtcggtgaag ttgtcgatga cctcctgcgg ataggcggcg tcgccactgt catcgggcgg
     2161 gaccgtcgag tcggcggggt catccgccgg ctcgtcgtcg gtgccgaaga tgtcaccgcc
     2221 gtcgttgccc gtgtcggcca tgtcgtcgaa gaggtccgac gccgacggat cgtccgtggc
     2281 gggcgtggtg tcctcgtccg acaccgtgac gtcagccgcg tcgccgccgt cgtcgtcgcc
     2341 gcccccgagg gtcaggacca gggcgacgac gatggcaacc aggacggcgc cgacgcccgc
     2401 gatgaggagg gccttgccgc tgccgcccga ggacgagctc tcgggcccgg cataggtgcc
     2461 cagcggggcc ccgccgcccg gtggcgggct accgggggcc ccggggggcg gccctgacgc
     2521 cgccgggctg acggaggtcg ctgcggggtc aggcgtggcg gccggggtcg gctgggtgga
     2581 cgcggcggcc gggccgccct gcacggggtc ggtgctgacg gcctggccgt ccgcgacgtg
     2641 gtcggtccag tcgttgccgt cgaaccagcg gttctggtgg cggttggtgg ggtccgggta
     2701 ccaacccgca ggggtgctca tggcggcgac tccttcgagg tcatgtcgtt cctgctccct
     2761 cccgcccggc gaccggccga cccgacggcc tcagggcttc gcgctgctga cgatccacat
     2821 cgcgaagaac tgcgcccctc caccgtaggc gtggccgagg gccgtccggg caccgtcgac
     2881 ctggtggtca ccgcacatcc ctcgcacctg catggccgcc tcgccgaagc ggatcatccc
     2941 cgacgccccg atggggttcg tggacaggac cccgcccgac gcgttccagg ggatgtcgcc
     3001 gtcgagggcc gtcgcccccg actcggtcag cttccacccc tcgccctcct ccgcgaagcc
     3061 gaggttctcc agccacatgg gctcgtacca ggagaagggg acgtagacct cggccagatc
     3121 gatctgttgg cgggggttgg tgatgcccgc ctgctggtag aggtcggcgg cgcagtcccg
     3181 tccggcctgg gggttcacgt ggtcccgacc ggcggccatg gtggcctcgc tgcgcaggga
     3241 cgagccgtgg agccacgccg ggttggggct ggcgtcgccg gtggcctcgt cggccagcac
     3301 catggcgaag gcgccgtccg acgacgggca ggtctcgtcg tagcggatcg ggtcccagag
     3361 catcatcgag tc
//
LOCUS       DASTFV010000665         2840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2520314, whole genome shotgun sequence.
ACCESSION   DASTFV010000665 DASTFV010000000
VERSION     DASTFV010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2520314"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VFP02_12215"
     CDS             <1..411
                     /locus_tag="VFP02_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543846.1"
                     /translation="PPDFDLDQALTSIAKFAARHPSGIALAHYGLVPDAAAVLDEATE
                     VLTRWAEVAEAAWRKGDDIADALDAAFGGDLADVDEVHREKLETLNGIHSNAAGFRRW
                     LDQRADQPPGHHHGSGPAPPAPRNASTQADDPPA"
     gene            408..2090
                     /locus_tag="VFP02_12220"
     CDS             408..2090
                     /locus_tag="VFP02_12220"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 protein"
                     /protein_id="HET9543847.1"
                     /translation="MRLELLRHEVHGADTRGLVVDVDQAGDRFTWSVANSADHPVAVH
                     QITLVFAVPDVRFPLRLFRNGYQSWSVTDAAAFGTDRDPSTADTLPFLRDMHHADRDP
                     AAAGELRSEQVTVLRDHGDRQALVGFIGGRHHDGTIRLRPTPSGLEVRAEATLGGAVL
                     DAGARRSLHPVVVRSGDDPAELLAEWAKDVGQAEHARVTAPYQVGWCSWYHYFDGVTE
                     ADITANLARAGEWPFDVFQLDDGYQAAVGDWLSTNEKFPSGVDGVAAAIAAAGFVPGL
                     WVAPFIAAPSSELALAHPEWLAREADDPTKPMIGMFNDIWGGFMYGLDVTRSDVLDHL
                     AQVAADLVDAGYCYLKLDFTFSAKQQGRYADATLTPAERVRGAYEAIRRGAGEDTFIL
                     GCGAPLGSLVGVVDGMRIGPDVGPHWATDPLTAPLPGYRETQPSTRGAWRSTLARAFL
                     HRQLWLNDPDCLMLRPSETHLTTEQARSWALAVGVSGGMAVVSDDLALLGPNEQALLD
                     EVLALGRASDAAARAGAPPRTPDLLDGPIPAVLEAAGHTLVGDPECGSASLT"
     gene            2155..2802
                     /locus_tag="VFP02_12225"
     CDS             2155..2802
                     /locus_tag="VFP02_12225"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9543848.1"
                     /translation="MAAPQNRWPDVDHALAYLEKADTIPHRAEGTAVFVELLPEPVER
                     VLDLGTGDGRLLDLVLTARPGSSGVGLDLNAEMLARARERFVGDDRVVIGEHDLDDPL
                     PDHGPFDAIVSSFAIHHVADERKRTLYVECFDRLRPGGAFLNLEHVASPTWGLHEAFL
                     NQLGLDPADDDPSNLLAPVDDQLGWLRDAGFTDVDCHWKWRELALLVGVRPAADR"
BASE COUNT          376 a         1086 c          965 g          413 t
ORIGIN      
        1 cctcccgact tcgacctgga ccaggccctg acgtcgatcg ccaagttcgc ggcccggcac
       61 ccgagcggca tcgccctggc ccactacggg ctggttcccg acgccgccgc cgtgctcgac
      121 gaggccaccg aggtcctcac ccgctgggcc gaggtggcgg aggccgcttg gcggaagggc
      181 gatgacatcg ccgacgccct ggacgccgcc ttcggtgggg acctggccga tgtggacgag
      241 gtccaccggg agaagttgga gaccctcaac ggcatccact cgaacgctgc cggcttccgt
      301 cgctggctcg accagcgcgc cgaccaaccg cctggccacc accacgggtc cgggcccgcc
      361 cccccggcgc cgaggaacgc gagcacccag gccgacgacc cgccggcgtg aggctggagc
      421 tgctgcgcca cgaggtgcac ggggccgaca cccgcggtct ggtggtcgac gtcgaccagg
      481 cgggcgaccg gttcacctgg tccgtggcca acagcgcgga ccatccggtc gccgtccacc
      541 agatcaccct cgtcttcgcc gtccccgacg tgcgcttccc gctgcgcctg tttcgcaacg
      601 gctaccagtc ctggtcggtg accgatgccg ccgccttcgg cacggaccgg gatccctcga
      661 ccgccgacac gctcccgttc ctgcgggaca tgcaccacgc cgaccgggat cccgccgccg
      721 ccggtgaact ccggtccgag caggtgacgg tcctgcggga ccacggcgac aggcaggccc
      781 tggtcggctt catcggtggt cgccaccatg acggcaccat ccgcctgcgg cccacgccgt
      841 caggcctcga ggtgcgggcc gaggccaccc tcggtggggc ggtcctcgat gccggggctc
      901 ggcgctccct tcatccggtg gtcgtccggt caggcgacga tccggcggag ttgctcgccg
      961 agtgggcgaa ggacgtcggc caggccgagc acgctcgggt cacggccccg taccaggtgg
     1021 gctggtgctc gtggtaccac tacttcgacg gcgtcaccga ggccgacatc acggccaacc
     1081 tggcccgcgc cggcgagtgg cccttcgacg tcttccagct cgacgacgga taccaggccg
     1141 cggtcgggga ctggctgagc accaacgaga agttcccctc gggcgtcgac ggcgttgccg
     1201 cggccatcgc cgccgccggg ttcgtgcccg gcctctgggt cgctccgttc atcgccgccc
     1261 cttcctccga gctggccctc gcccatcccg agtggctggc tcgggaggcc gacgacccga
     1321 cgaagcccat gatcgggatg ttcaacgaca tctggggtgg gttcatgtac ggcttggacg
     1381 tcacccggtc cgatgtcctc gaccacctgg ctcaggtcgc cgccgacctg gtcgacgccg
     1441 ggtactgcta cctgaaactg gacttcacct tcagcgccaa gcagcaaggg cgctacgccg
     1501 acgccaccct cacgccggcc gagcgggtac ggggggccta cgaggccatt cgccgcgggg
     1561 ccggcgagga caccttcatc ctgggctgcg gcgccccgtt gggctcgctg gtcggtgtgg
     1621 tcgacgggat gcgcatcggc ccggacgtcg gcccccactg ggccacggat cccctgaccg
     1681 ccccgctgcc gggctaccgc gagacccaac cgtcgacccg gggggcgtgg cgatcgacct
     1741 tggcccgggc cttcctgcac cgtcagctgt ggctcaacga ccccgactgc ctcatgctgc
     1801 gcccgtccga gacacacctg acgaccgaac aggcccggtc ctgggccctg gcggtcggtg
     1861 tctcgggcgg catggccgtc gtgtccgatg atctggccct gctcggcccg aacgagcagg
     1921 ccctgctcga cgaggtcctg gccctcggac gagcgtccga cgccgcggcc cgggccgggg
     1981 cgccgccccg gaccccggac ctgctcgacg gcccgatccc cgccgtcctg gaggctgccg
     2041 gccacaccct cgtcggcgat cccgaatgcg gctccgcctc gctgacctga tcccgaccgc
     2101 gatctccgcg gtaatccggc cccgctgagg gccgcgccgc gggtaagaac gcggatggct
     2161 gctccgcaga accgttggcc cgacgtcgac cacgcgctgg cctacctcga gaaggcggac
     2221 accatcccgc accgggcgga gggcacggcc gtcttcgtgg agctgcttcc cgaaccggtc
     2281 gagcgcgtgt tggacctggg cacgggcgac ggtcgcctgc tcgacttggt gctgacggcc
     2341 cgcccggggt cgagcggcgt cggcctggac ctcaacgccg agatgctggc ccgggcccgt
     2401 gagcgcttcg tcggtgacga tcgggtcgtg atcggcgagc acgacctgga cgacccgctc
     2461 cccgaccacg gcccgttcga cgcgatcgtg tccagcttcg ccatccacca cgtggccgac
     2521 gagcgcaagc gcaccctcta cgtggagtgc ttcgaccggc tccggcccgg cggggccttc
     2581 ctgaacctgg agcacgtggc ctcgccgact tggggcctgc acgaggcctt cctgaaccag
     2641 ttggggctcg accccgccga cgacgacccg tccaacctcc tggctcccgt cgacgaccag
     2701 ctcgggtggc tgcgggacgc cggtttcacc gacgtcgact gccactggaa atggcgggag
     2761 ctggccctcc tcgtcggcgt ccgcccggcc gcggatcggt agggtcggca tcatgctgat
     2821 cggtggccat gtcgagcccg
//
LOCUS       DASTFV010000666         2064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2523452, whole genome shotgun sequence.
ACCESSION   DASTFV010000666 DASTFV010000000
VERSION     DASTFV010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2523452"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFP02_12230"
     CDS             complement(<1..504)
                     /locus_tag="VFP02_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543849.1"
                     /translation="MRHRTRHPALLAILLGVPLLVLAACGDADPTGAPAAAATIPAGG
                     PLLLPTATGLASVEAASGTVAFEAPGAKASPDGSVVVRGVIRADGSTRLTWLDPVDGR
                     PIARVRLDEPASLRVVAADGRVAVGPPDVPVTGLIYPVARAQSPVSVVAPDGTVHTYT
                     LPGSVEPE"
     gene            640..1179
                     /locus_tag="VFP02_12235"
     CDS             640..1179
                     /locus_tag="VFP02_12235"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9543850.1"
                     /translation="MEEPDPVLVRAAAEGDRDAFDALVRLYQQPVWRFLCHLVADPAL
                     AEDLAQETFLRAYRNLGTFAHQSRFSTWLFQIARNAGIDALRHRERGARLLEVVPVAD
                     SRPSPEAGAELEAGLAALSPKLREALLVVEVLGYRYREAAIILGVPEGTVKSRVHQAR
                     VRLVDWMDDEADDAGRAAR"
     gene            1176..>2064
                     /locus_tag="VFP02_12240"
     CDS             1176..>2064
                     /locus_tag="VFP02_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543851.1"
                     /translation="MSCTEARTWVSERLDGGDPPALSEHLDGCADCRHFVDQTEALRR
                     SLRFEAVGTVPDIAGAVRAHLEADRSGPWDSRPTTNDRFQNEGRGGRRGRHRSTIAVA
                     AAALVAGVLIGVNLVGVGDDRPRPVAAESLPARVASAQATVDGLHADLRLVERGWNPA
                     VPERRYQGTLDYRAPESLALQWQDQTAYPSGAWRPNDVSLRTDGAAWVSTGVPDCQSF
                     AQPDCSLPPRERSETGRPPFADSTPVPLELVVPVRSFTSTDTATVVGEGSVAGRPTIE
                     VTVAAAQVGPLLDSLRPAGN"
BASE COUNT          256 a          772 c          782 g          254 t
ORIGIN      
        1 ctccggctcc acgctgcccg ggagggtgta ggtgtgcacg gtcccgtccg gggcgacgac
       61 gctcaccggg ctctgggccc gggcgacggg atagatgagg ccggtcaccg ggacgtcggg
      121 cggtccgacg gcgacccggc cgtcggcggc cacgacccgc aggctggcgg gctcgtccag
      181 gcggacccgg gcgatgggac ggccgtcgac ggggtccagc caggtcaggc gggtggagcc
      241 gtcggcccgg atgacacccc ggaccaccac cgaaccgtcc ggggacgcct tcgcccccgg
      301 tgcctcgaag gcgacggtgc cgctggctgc ctcgacggag gcgaggccgg tggccgtggg
      361 cagcaggagt gggccgcccg cggggatggt ggccgccgcg gcgggggcgc cggtgggatc
      421 ggcgtcgcca caggcggcca ggaccagcag cgggacgccg aggaggatgg ccaggagggc
      481 gggatggcgg gttcggtgtc gcacaccccg tgagacgccg cccgacggtc cagggttcac
      541 cgaaccattc cgcccgtgcc gtcgtctcaa cccccgtgga cgtcggaccg ggcccgaacg
      601 ggtggacgcc gcccgttggc cgcgagtagg ccgggtcggg tggaggagcc ggatccggtc
      661 ctggtccggg ctgcggccga aggtgaccgg gacgcgttcg acgcgctggt acgcctctac
      721 cagcagccgg tctggcgctt cctgtgccac ctcgtggccg atcccgcgct ggccgaggac
      781 ctcgcccagg aaaccttcct gcgggcctac cggaacctcg ggaccttcgc gcaccagtcc
      841 cggttctcga cctggctgtt ccagatcgcc cgcaacgccg gcatcgacgc cctgcgccac
      901 cgtgagcggg gcgcccgcct gctcgaggtc gtccccgtcg ccgactcccg gccctcgccg
      961 gaggccgggg ccgagttgga ggccggcctc gccgcgctca gcccgaagct gcgcgaggcc
     1021 ctgctcgtgg tcgaggtcct cggttaccgg taccgggagg ccgcgatcat cctcggggtg
     1081 cccgagggca cggtgaagag ccgggtccac caggcccggg tgcgcctcgt cgactggatg
     1141 gacgacgagg ccgacgacgc cgggcgggcg gcccgatgag ctgcaccgag gcccgcacct
     1201 gggtgtccga gcgcctcgac ggcggcgatc cccccgccct gtccgaacac ctcgacggct
     1261 gtgccgactg ccgccacttc gtcgaccaga ccgaggccct gcgtcgttcg ctccgcttcg
     1321 aagccgtcgg caccgtcccc gacatcgccg gcgccgtccg cgcccacctc gaagccgacc
     1381 gttctggacc ctgggactcg cggcccacca caaacgacag gttccagaac gaggggcggg
     1441 gcgggaggcg ggggcggcac cggtcgacga tcgccgtggc ggcggcggcg ttggtcgcgg
     1501 gggtgctgat cggggtgaac ctggtcggcg tgggcgacga ccggccccga ccggtcgccg
     1561 ccgagagcct tccggcccgc gtcgcctcgg cccaggccac ggtcgacggc ctgcacgccg
     1621 acctgcgcct ggtcgagcgg ggctggaacc ccgccgtccc cgagcgtcgc taccagggca
     1681 cgctggacta ccgggccccc gagtccctgg ccctccagtg gcaggaccag acggcgtacc
     1741 cgagcggggc ttggcggccc aacgacgtca gcctgcggac cgacggggcg gcatgggtgt
     1801 cgaccggtgt acccgactgc cagtccttcg cccagcccga ctgctcgctg ccgccccgag
     1861 agcgatccga gacgggtcgt ccgcccttcg ccgacagcac ccccgtgccc ctcgagctgg
     1921 tggtgccggt gcgcagcttc acctcgaccg acaccgcgac cgtggtgggc gagggctcgg
     1981 tcgccgggcg tccgaccatc gaggtgacgg tggcggcggc ccaggtcggc cccctgctgg
     2041 acagcctccg cccggcgggg aacc
//
LOCUS       DASTFV010000667         3183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2535101, whole genome shotgun sequence.
ACCESSION   DASTFV010000667 DASTFV010000000
VERSION     DASTFV010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2535101"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VFP02_12245"
     CDS             <1..753
                     /locus_tag="VFP02_12245"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HET9543852.1"
                     /translation="ESIPLIASAIMSKKIAEGTDALVLDVKFGSGAFLPDPERGRELA
                     RTMVGLGRAHGVRTTALLTAMEQPLGRTAGNAVEVEESVEVLAGGGPEDLVEVTLALA
                     REMVELAGLDVDPADALTDGRAMDRWRAIVAAQGGDPDAPLPVAAENEVLTAGGDGIV
                     QRLDAMAVGVAAWRLGAGRARKEDDVSATAGVICHAKVGDRVRAGDPVLELRADDPSR
                     FAAAREALTDAWTLGADPADSGPLILDRITPD"
     gene            complement(767..1453)
                     /locus_tag="VFP02_12250"
     CDS             complement(767..1453)
                     /locus_tag="VFP02_12250"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9543853.1"
                     /translation="MRDYGQYCPVALGSEVLADRWTPLILREMVLGSTRFNDIERGLP
                     GISRSLLVQRLRHLERKEVLERRPAAGGRGSEYHLTEAGEDLGGVIMSLGEWAVRWKL
                     AEPELEEVDPRVLTWWMHLRMDEERLPDRRVVIEFAYQVPRIQIWIVLDRGDVSVCAQ
                     HPGFDSDLVVRTDAVSMARVFNGLTTLGQAVDEGTVELLGPPALVRGFGRWFLWSPFR
                     PAVQAELARR"
     gene            1513..2670
                     /locus_tag="VFP02_12255"
     CDS             1513..2670
                     /locus_tag="VFP02_12255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9543854.1"
                     /translation="MSTIERETESEREQQLVAWAGELGREVAEHAERHDRDGTFVVEA
                     YQLLADSGFLALAVPAELGGRGATVRQVAMAQRELARHCGSTALASVMHQHVVLFTAW
                     RHRRDMPGAEATLRRVADEGIVLVSTGGGDLTNPRGSATRVDGGFLVSGRKPFASQAP
                     VGAAMSTMFPYDDPEEGRVVLNMAIPTSSEGVTVLETWDALGMRGTGSHDIELKDVFV
                     SDQQVLARRPHGRIDGPLQVILSIAMPPIAAVYLGVAEAARDHAVGLIAGTARAGDTL
                     RQRQIGLMDTRLRVAAWALDGALDMVGDDPTPSMDTVVAVMAAKREIALAAVEVCQLA
                     LEVGGGAAFSRTQPIERCLRDVQAARYHPLDPEESLVHAGKVALGLPADER"
     gene            2703..>3183
                     /locus_tag="VFP02_12260"
     CDS             2703..>3183
                     /locus_tag="VFP02_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET9543855.1"
                     /translation="MLSSAQQADYRRDGFLVLPGFVSLEACAELKAEAEALVDGFEPD
                     AGVKSIFTTNEQTRHSDEYFLTSGDTTRFFFEEEAFDEDGGLRQAKALSINKLGHAMH
                     DLDPVFEHFSHTPDLAEVCTDLGYEDPSILQSMYIFKQPRIGGEVTSHTDHTFLWTEP
                     "
BASE COUNT          468 a         1164 c         1119 g          432 t
ORIGIN      
        1 gagtccatcc ccctgatcgc ctcggcgatc atgagcaaga agatcgccga gggcactgac
       61 gccctcgtcc tcgatgtgaa gttcggctcg ggcgcatttc tgcccgatcc cgaacggggc
      121 cgggagctgg cccgaacgat ggtcgggctg ggccgcgctc acggggtgcg gaccactgcg
      181 ctgctgacgg ccatggaaca gccgctgggg cgcacggcgg gcaatgccgt cgaggtcgag
      241 gagtccgtcg aggtgctggc cgggggcggg cccgaggacc tggtcgaggt caccctggcc
      301 ctcgccaggg agatggtcga gttggccgga ctggacgtcg acccggccga cgcgctcacc
      361 gacgggcggg ccatggaccg ctggcgggcc atcgtggccg cccagggcgg ggacccggac
      421 gctcccctgc ccgtcgccgc cgagaacgag gtgctgaccg ccggcggcga cgggatcgtc
      481 cagcggctcg acgccatggc ggtcggcgtg gccgcctggc gcctgggtgc gggccgggcc
      541 cgcaaggagg acgacgtcag cgccaccgcc ggcgtcatct gccacgccaa ggtgggcgac
      601 cgggtccggg cgggtgaccc ggtgctggag ctgcgggccg acgacccgtc ccgcttcgcc
      661 gcggcgcgtg aggccctgac cgacgcctgg accctcggcg ccgatcccgc cgactccggc
      721 cccctgatcc tggaccgcat cacccccgac tgagctcgtg gggcggctac ctccgggcca
      781 gctcggcctg gacggcaggg cggaacgggc tccacaggaa ccagcgaccg aagccgcgaa
      841 ccaaggccgg gggtccgagg agctccaccg tcccttcgtc gaccgcctgc cccagggtgg
      901 tcaacccgtt gaagacccgg gccatcgata ccgcgtcggt gcgcaccacg aggtcgctgt
      961 cgaagcccgg gtgctgggca cacacggaca cgtcgccgcg gtccagcacg atccagatct
     1021 ggatccgggg gacctggtag gcgaactcga tcaccacccg acggtcgggg aggcgctcct
     1081 cgtccatccg gaggtgcatc caccaggtga gcacccgggg atccacctcc tcgagctcgg
     1141 gctcggccag cttccagcgc acagcccact cgcccagcga catgatcacg ccgccgaggt
     1201 cctcgccggc ctcggtgagg tggtactcgc tgccccggcc gccggccgcc ggtcgccgtt
     1261 cgagcacctc cttgcgctcg aggtggcgca accgctgcac caacaggctg cgggagatgc
     1321 ccggcagccc ccgctcgatg tcgttgaacc gggtgctgcc gaggaccatc tcccgcagga
     1381 tcagcggggt ccagcggtcg gcgagcacct cgctcccgag ggcaaccggg cagtactggc
     1441 cgtagtcacg caccccgcca aggtacagat ccagcactgg ttgggtcggt ccgtcctctt
     1501 cacactgcct ccatgagcac catcgaacga gaaaccgaat ccgagagaga gcagcagctc
     1561 gtcgcgtggg ccggggagct cggccgggaa gtcgccgagc acgccgagcg ccacgaccgt
     1621 gacgggacct tcgtcgtcga ggcctaccaa ctgctggccg acagcggctt cctggccctg
     1681 gccgtccccg cggagctcgg gggccggggc gccacggtgc ggcaggtggc gatggcgcag
     1741 cgcgagctgg cccgccactg cgggtcgacg gccctggcgt ccgtcatgca ccagcacgtc
     1801 gtcctgttca cggcgtggcg tcaccggcgc gacatgccag gggctgaagc gacccttcga
     1861 cgggtcgccg acgagggcat cgtcctggtc tcgaccggcg gcggtgacct gaccaacccg
     1921 cggggctcgg ccacccgggt cgacggcggt tttctggtca gtggccgcaa gccgttcgcc
     1981 agccaggccc ccgtgggggc cgcgatgtcg accatgttcc cctacgacga tcccgaggag
     2041 ggccgggtcg tgctgaacat ggcgatcccc acgtcgagcg agggtgtcac ggtcctcgag
     2101 acctgggacg ccctcggcat gcgggggacc ggcagccacg acatcgagct gaaggacgtc
     2161 ttcgtcagcg accagcaggt cctggcccgc cggccccacg gccggatcga cgggcccctg
     2221 caggtcatcc tcagcatcgc catgccgccc atcgccgcgg tctacctggg cgtggccgag
     2281 gccgcccggg accatgccgt cgggctcatc gccggcacgg cacgggccgg cgacaccctg
     2341 cgccagcgcc agatcggcct gatggacacc cgtctgcggg tcgccgcctg ggccctcgac
     2401 ggtgccctcg acatggtggg cgacgacccg accccgtcca tggacacggt ggtggccgtg
     2461 atggccgcca agcgggagat cgccctcgcc gccgtcgagg tctgccagct ggcgctcgag
     2521 gtcgggggcg gcgccgcctt ctcccggacc cagcccatcg agcggtgcct ccgcgacgtg
     2581 caggccgccc ggtatcaccc cctcgatccg gaggagtcgc tggtccacgc cgggaaggtc
     2641 gccctcggcc tccccgccga cgagcgctga tcaccggctc ggcaccgggc tacgttggcc
     2701 cggtgctgag ctcggcgcag caggcggact accggcggga cgggttcctc gtcctccctg
     2761 ggttcgtgtc gctcgaggcc tgtgccgagc tcaaggccga ggccgaagcg ttggtcgacg
     2821 gcttcgagcc cgacgccggg gtgaagtcca tcttcacgac gaacgagcag acccggcaca
     2881 gcgacgagta cttcctcacc tcgggcgaca cgacccgctt cttcttcgag gaggaggcgt
     2941 tcgacgagga cggcgggctg cgccaggcga aggccctgtc gatcaacaag ctgggccatg
     3001 ccatgcacga cctggacccg gtgttcgagc acttcagcca cacgccggac ctggctgagg
     3061 tctgcacgga cctgggctac gaggacccgt cgatcctcca gtcgatgtac atcttcaagc
     3121 agccccgcat cggtggcgag gtgacgagcc acaccgacca caccttcctg tggaccgaac
     3181 ccc
//
LOCUS       DASTFV010000668         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2535697, whole genome shotgun sequence.
ACCESSION   DASTFV010000668 DASTFV010000000
VERSION     DASTFV010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2535697"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VFP02_12265"
     CDS             <1..638
                     /locus_tag="VFP02_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9543856.1"
                     /translation="GDGFCSGLDLEDAGVPPHADGLTLSRLAIRAMEYFGDIVPQRRK
                     LPQPVIGAINGPAIGGGMCLSLGTDIRIAGQSAYWRAAGINNGLGAVELGLSWTLSRA
                     IGSSRAFEICLSGRDVHAEEAERIGLVSRTVPDGDLLDTTYELAERICGFSTHGVSMT
                     KKVLWSALEVGSLEAALDMENRNQLLVRLTTQNLEEYIRSRREGRTPTYLD"
     gene            complement(706..1068)
                     /locus_tag="VFP02_12270"
     CDS             complement(706..1068)
                     /locus_tag="VFP02_12270"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET9543857.1"
                     /translation="MSDIVKINAIRVPEGMGPTIEERFAARGDAMNDVEGFVSFELLR
                     PTAGEDRYFVYTRWETEAAFQAWRESQRFAESHAGQTGPPPEGHGGPAKDPGEGSGPV
                     GMGADLLEFEVALAVTKA"
     gene            complement(1142..1672)
                     /locus_tag="VFP02_12275"
     CDS             complement(1142..1672)
                     /locus_tag="VFP02_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET9543858.1"
                     /translation="MGTGIEPDAVNLALLGLRLILGAVMLAHGINHIIGGGKIAGTGR
                     WFESLGMKPGPLHAWLASLTEVGAGAMLVLGLLTPLACAGVLGVMLVALVTNHWKNGF
                     FIFRPGEGYEYVLTLIVVSLCLALLGSGQWSVDDALETTDLAGWSGLWIAVLAGGGGT
                     AVLLAVFWRPPVPKAE"
     gene            1697..>2079
                     /locus_tag="VFP02_12280"
     CDS             1697..>2079
                     /locus_tag="VFP02_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9543859.1"
                     /translation="MHLDLLVDPFDATWPQVRGLAVAAEEQGYGGVWTWDHLSGVVHG
                     QDHVLEAWTLLSALAAVTSRVMLGPLVLNQANRDAGVVAQMAATLQEVSGGRLLLGLG
                     AGTSPTGSYVAEQLALGRVPAHDPER"
BASE COUNT          308 a          733 c          733 g          305 t
ORIGIN      
        1 agggcgacgg gttctgctcc gggctggacc tggaggacgc cggggtccca ccccacgccg
       61 acgggctgac cctgtcccgc ctggccattc gggcgatgga gtacttcggc gacatcgtcc
      121 cgcagaggcg gaagctgccc cagccggtga tcggggcgat caacggcccg gccatcgggg
      181 gcggcatgtg cctgtcgctg gggaccgaca tccgcatcgc cggtcagtcg gcctactggc
      241 gggcggcggg gatcaacaac ggcctcggtg cggtcgagct gggcctctcg tggacgctgt
      301 cgcgggccat cgggtcgtcc cgggcgttcg agatctgcct gtcgggccgc gacgtgcacg
      361 cggaggaggc cgagcgcatc ggcctggtgt cccgcacggt gcccgacggc gatctgttgg
      421 acacgaccta cgagctggcc gaacgcatct gcgggttcag cacccacggg gtgtcgatga
      481 ccaagaaggt gctgtggtcc gccctcgagg tcggcagcct ggaggcggcg ctggacatgg
      541 agaaccggaa ccagctgctg gtccgcctga ccacccagaa cctggaggag tacatccgct
      601 cccgccgcga gggccggaca ccgacctacc tcgactgatc ctgaacttgc gtcggctaca
      661 ccacagatct gtggttcatt cgacgcaggt tcgggcggag gggggtcagg ctttggtgac
      721 ggcgagggcg acctcgaact ccagcaggtc ggcccccatg ccgacggggc cgctgccctc
      781 accggggtcc ttggccgggc cgccgtggcc ttcggggggc ggtccggtct ggccggcgtg
      841 ggactcggcg aagcgctggc tctcccgcca cgcctggaag gcagcctcgg tctcccagcg
      901 ggtgtagacg aagtagcggt cctcgccggc ggtcggccgc agcagctcga aggacacgaa
      961 gccctcgacg tcgttcatgg cgtcgccccg cgccgcgaag cgctcctcga tggtcgggcc
     1021 catgccctcg ggcacccgga tggcgttgat cttcacgatg tcactcatcc cgcccgaccg
     1081 tatccatccg taccttgtgc acctgtgcgc caaagtggcg gactggtgca ccaggtacga
     1141 gtcactcggc cttggggaca gggggtcgcc agaagaccgc caggagtacg gcggtcccac
     1201 cgccgccggc gaggaccgcg atccacagcc cactccaacc cgccaggtcc gtcgtctcca
     1261 gggcatcgtc gaccgaccac tggccggagc cgagcagagc cagacagagg ctgaccacga
     1321 tcagggtcag gacgtactcg tagccctcgc ccgggcggaa gatgaagaag ccgttcttcc
     1381 agtggttggt gacgagcgcc accagcatca cgcccaggac gccggcgcac gccagcgggg
     1441 tgagcaggcc gaggacgagc atcgcccccg cgccgacctc ggtcaggctg gccagccagg
     1501 cgtggagggg ccccggcttc atgcccaggc tctcgaacca gcggccggtc cccgcgatct
     1561 tgccgccacc gatgatgtgg ttgatcccgt gggccagcat cacggctccc aggatcagac
     1621 gcagcccgag cagcgccagg ttgaccgcat ccggttcgat ccctgttccc atggcctgac
     1681 cctacgctcg gccgggatgc acctggacct cctcgtcgat ccgttcgatg ccacctggcc
     1741 tcaggtccgc ggcctggccg tcgccgccga agaacagggc tacggcggag tgtggacctg
     1801 ggaccacctg tcgggcgtcg tccacggcca ggaccatgtg ctcgaggcgt ggaccctgct
     1861 gtcggcgctg gccgcggtga ccagccgggt catgctcggg ccgctggtcc tgaaccaggc
     1921 caaccgggac gccggggtgg tggctcagat ggcggccacc ctgcaggagg tgtccggcgg
     1981 acggctgctg ctcgggctcg gcgccggcac cagcccgacc ggctcctacg tcgccgagca
     2041 gcttgccctg ggccgggtgc ccgcccacga ccccgagcg
//
LOCUS       DASTFV010000669         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2539660, whole genome shotgun sequence.
ACCESSION   DASTFV010000669 DASTFV010000000
VERSION     DASTFV010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2539660"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFP02_12285"
     CDS             <1..373
                     /locus_tag="VFP02_12285"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543860.1"
                     /translation="EHVGEVFDAEHTRLFTFALDTEHELVNLRAVVQGRATRVRAEEV
                     PAGGPVPAAAATGTTTIWVGDGEREATVYDRALLQAGNHVVGPAVVTEMDSTTLILPD
                     HAGEVDRYGNLLLRPASSEGS"
     gene            377..>2079
                     /locus_tag="VFP02_12290"
     CDS             377..>2079
                     /locus_tag="VFP02_12290"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET9543861.1"
                     /translation="MATVIETNPRQFATVEVDPVTVDIIENALRNTRYEMDAVLFRTA
                     MSPGIREQHDEFPIIADRAGRMVVGQFGSFIDGFLTTYQGEISEGDVFLTSDPYACDG
                     AISHANDWLVLLPIYREGALVGWAAMFGHMTDVGGKVPGSLPTDATSIYEEGVVVPPT
                     KLYRRGELATEALELVLNQVRMKTWNRSDLNAIVAACRTAARRVLELCDRFGTETYLS
                     TLDALLDRNYRAMAQLIRTAVPTERLEFTDYVCDDGRGFGPYKIHCSMQRVGDKVVLD
                     FAGTDPQAEGSINFYLNENMLKMFFGIYMIMVFDPQILWNDGFYPLVEVNIPEGSLLK
                     PRYPAALSCRTHALGRIFDVMGGLLGQRAPEFLCAAGFSSSPHLMYSGVDRNGEWFQL
                     YQIGFGGIPGRPFGDGPDGHSLWPSFTNVPNEFLESYFPLVIERYQTVADSGGPGFFR
                     GGNGIDITYRFLEPGEVSIHDDRWFTYPFGVNGGLPGARSRKLLRRTDGTEQVLPSKC
                     DHVKVGVGDTLQYMTWGGGGWGDPFDRDPALVALEVDRGLVSVEGAARYGVVLTDAGA
                     V"
BASE COUNT          311 a          785 c          675 g          308 t
ORIGIN      
        1 ggagcatgtc ggcgaggtgt tcgacgccga gcacacccgg ctgttcacct tcgccctcga
       61 caccgagcac gagctggtga acctccgggc ggtggtccag ggccgggcca cccgggtccg
      121 ggccgaggag gtcccggccg gtggccccgt cccggcggcc gcggccaccg gcaccaccac
      181 catctgggtc ggcgacggcg agcgggaggc caccgtctac gaccgggcgc tcctgcaggc
      241 cggcaaccac gtcgtggggc cggcggtggt caccgagatg gactcgacca cgctgatcct
      301 tcccgaccac gccggcgagg tcgaccgcta cggcaacctg ctgctccgac cggcctcgag
      361 cgaagggagc taggccatgg ccaccgtcat cgagaccaac ccgcgccagt tcgccaccgt
      421 cgaggtcgac ccggtcaccg tcgacatcat cgagaacgcc ctgcgcaaca cccgctacga
      481 gatggacgcg gtgctgttcc gcacggccat gtcgcccggc atccgcgagc agcacgacga
      541 gttcccgatc atcgccgacc gcgccggccg catggtcgtg ggccagttcg gcagcttcat
      601 cgacggcttc ctcaccacct accagggcga gatctccgag ggcgacgtct tcctcacctc
      661 cgacccgtac gcctgcgacg gcgccatcag ccacgccaac gactggctgg tcctgctgcc
      721 catctaccgg gagggggcgc tggtcggctg ggcggccatg ttcggccaca tgaccgacgt
      781 gggcggcaag gtcccgggca gcctgcccac cgacgccacc tccatctacg aggagggcgt
      841 ggtcgtcccc ccgaccaagc tgtaccgccg gggggagctg gccaccgagg ccctggagct
      901 ggtcctcaac caggtccgca tgaagacctg gaaccgcagc gacctcaacg ccatcgtggc
      961 cgcctgccgg accgccgccc gccgggtcct tgagctgtgc gaccgcttcg gcaccgagac
     1021 ctacctgtcg accctcgacg ccctgctgga ccgcaactac cgggccatgg cccagctcat
     1081 ccggaccgcc gtccccaccg agcggctcga gttcaccgac tacgtctgcg acgacggccg
     1141 cggcttcggc ccctacaaga tccactgctc catgcagcgg gtcggggaca aggtggtgct
     1201 cgacttcgcc ggcaccgacc cgcaggccga gggctcgatc aacttctacc tgaacgagaa
     1261 catgctgaag atgttcttcg gcatctacat gatcatggtc ttcgaccccc agatcctctg
     1321 gaacgacggc ttctacccgc tggtcgaggt caacatcccc gaggggtcgc tgctcaagcc
     1381 ccgctacccg gccgccctgt cgtgccggac ccatgccctg ggccggatct tcgacgtcat
     1441 gggcgggctg ctcggccagc gggcgcccga gttcctctgc gcggccggct tctcctcgtc
     1501 cccgcacctg atgtactcgg gggtcgaccg caacggcgag tggttccagc tctaccagat
     1561 cggcttcggc ggcatcccgg gccggccctt cggcgacggg cccgacggcc attcgctgtg
     1621 gccgtcgttc accaacgtcc ccaacgagtt cctggagagc tacttcccgc tggtcatcga
     1681 gcgctaccag acggtggcgg actccggcgg cccggggttc ttccggggcg gcaacggcat
     1741 cgacatcacc taccggttcc tggagccggg cgaggtgtcg atccacgacg accgctggtt
     1801 cacctacccg ttcggggtca acggcggcct gcccggcgcc cgcagccgca agctgctgcg
     1861 ccgcaccgac ggcaccgagc aggtgctgcc ctccaagtgc gaccacgtca aggtcggcgt
     1921 cggcgacacc ctccagtaca tgacctgggg cgggggcggc tggggcgacc ccttcgaccg
     1981 cgacccggcc ctggtcgccc tggaggtcga ccgcggcctg gtctcggtgg agggggcggc
     2041 acgctacggc gtggtcctga ccgacgccgg cgctgtcga
//
LOCUS       DASTFV010000670         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2542732, whole genome shotgun sequence.
ACCESSION   DASTFV010000670 DASTFV010000000
VERSION     DASTFV010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2542732"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFP02_12295"
     CDS             complement(<1..285)
                     /locus_tag="VFP02_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9543862.1"
                     /translation="MEHPIEASGLVRHFGDVVAVDGVDLQVREGEIYGFLGPNGAGKS
                     TTVRMLTTLLSPTAGSARVAGFDVVKEAGKVRRSIGVALQDAAIDPLMSGR"
     gene            complement(337..696)
                     /locus_tag="VFP02_12300"
     CDS             complement(337..696)
                     /locus_tag="VFP02_12300"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET9543863.1"
                     /translation="MPKPRKTTIALVLATIVAVGYVMFVPARNYMNQQAATDAAEHQL
                     DALDAELEELNARQVALQDPEQIEQVARERFNLGYPGEEVFAVLPAPPAPLPIPTGWP
                     FDVLRGAAAAAASGTAG"
     gene            complement(689..1981)
                     /gene="eno"
                     /locus_tag="VFP02_12305"
     CDS             complement(689..1981)
                     /gene="eno"
                     /locus_tag="VFP02_12305"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET9543864.1"
                     /translation="MSEIIHVVGREVLDSRGNPTVEVEVVLASGARGRAIVPSGASTG
                     QFEAVELRDGGDRYGGKGVLDAVGFVNGELTDLLLGEEALDQRDVDRQLIDADGTDNK
                     ARVGANAILGISLAVAKAAADELEVPLYRYVGGANAHVLPVPMMNVINGGEHADNNLD
                     VQEFMILPVGAASFAEALRWGAETYQALKGILSRRGLSTLVGDEGGFAPNLASNEEAV
                     ALLVEAIEAAGRTPGDDIVLGLDAASTEFFEDGTYVLAGEGGRRLSSDDMVDYLAGLV
                     DRYPIVTIEDGMAEEDWDGWSALTERLGRSLQLVGDDLFVTNTERLERGIQSGVANSI
                     LVKVNQIGSLTETLDAVDMAHRAAYTAVMSHRSGETEDTTIADLAVATNCGQIKTGAL
                     ARSDRVAKYNQLLRIEEDLGEAAAYPGATAFPGGGSDA"
     gene            complement(2107..>2365)
                     /locus_tag="VFP02_12310"
     CDS             complement(2107..>2365)
                     /locus_tag="VFP02_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543865.1"
                     /translation="EKGRDSVFDGIPRGLPALLFALKIEKKAAATGLLDRVPPPAGND
                     LGAELLAIVTRARDAGIDPEDALRRTAAAWRDALRAAESAG"
BASE COUNT          318 a          822 c          851 g          374 t
ORIGIN      
        1 ccggccgctc atgagggggt cgatggcggc gtcctgaagg gccacaccga tcgaccggcg
       61 gaccttgccg gcctccttga ccacgtcgaa tccggcgacc cgggccgagc cggccgtggg
      121 gctgagcagg gtcgtcagca tccgcacggt ggtcgacttg ccggccccgt tgggacccag
      181 gaacccgtag atctcgccct cgcggacctg aaggtcgaca ccgtcgacgg cgaccacgtc
      241 gccgaagtgc cgaaccagcc cactggcttc gatcggatgt tccacgacgc tccccacctc
      301 ggcggtaggg cgaatctatc cagctggcag tggctgtcag ccggccgtgc cggatgccgc
      361 ggccgcggcc gcgccacgga ggacgtcgaa gggccaaccg gtggggatgg gcagcggcgc
      421 agggggggcg ggcaggacgg cgaagacctc ttcaccgggg tagccgaggt tgaaacgttc
      481 ccgggccacc tgctcgatct gctccgggtc ctggagcgcg acctgcctgg cgttcagctc
      541 ctcgagctcc gcatcgaggg cgtccagctg gtgctcggcg gcgtcggttg ccgcttgctg
      601 gttcatgtag ttccgggcgg gtacgaacat cacgtaaccc acggccacga tcgtggccag
      661 gaccagagcg atcgtcgtct tcctcggctt aggcatccga ccctcccccc gggaaggcag
      721 tggcgcccgg ataggccgcg gcctcgccga ggtcctcctc gatccgcagg agctggttgt
      781 acttggcgac ccggtcgctg cgggccaggg ccccggtctt gatctggccg cagttggtgg
      841 ccacggccag atcggcgatg gtcgtgtcct cggtctctcc ggagcggtgc gacatgaccg
      901 cggtgtaggc cgcccggtgg gccatgtcga cggcgtcgag ggtctcggtc agcgaaccga
      961 tctggttgac cttgaccagg atggagttgg ccaccccgga ctggatcccc cgctcgaggc
     1021 gctcggtgtt ggtgacgaag aggtcgtcgc cgaccagctg cagcgatcgg ccgaggcgct
     1081 cggtgagggc ggaccagccg tcccagtcct cttcggccat gccgtcctcg atggtcacga
     1141 tcgggtaccg gtcgaccagg ccggccaggt agtcgaccat gtcgtcggag gagagccgtc
     1201 ggccgccctc gccggccagg acgtaggtgc cgtcctcgaa gaactccgtc gacgcggcgt
     1261 ccaggcccaa cacgatgtcg tcgcccgggg tccggcccgc ggcttcgatg gcctccacca
     1321 gcagggccac ggcctcctcg ttcgaggcca ggttcggggc gaagccgccc tcgtcgccca
     1381 ccagggtcga caggccccgc cgcgacagga tgcccttgag cgcctggtag gtctcggccc
     1441 cccagcgcaa cgcctcggcg aaggaagccg ccccgacggg caggatcatg aactcctgga
     1501 cgtccaggtt gttgtccgcg tgctcgccac cgttgatcac gttcatcatc ggcacgggca
     1561 ggacatgggc gttggccccg ccgacgtagc ggtagagggg gacctcgagc tcgtcggcgg
     1621 ccgccttggc cacggccagg gagatcccga ggatggcatt cgccccgacc cgggccttgt
     1681 tgtccgtccc gtcggcgtcg atcagctgcc ggtcgacgtc gcgctggtcg agcgcttcct
     1741 cgccgagcag caggtcggtg agctcaccgt tgacgaagcc cacggcgtcg aggacgccct
     1801 tgccgccgta gcggtcgcca ccgtcccgca gctcgacggc ttcgaactgg ccggtggacg
     1861 ccccactggg gacgatggcc cggccccggg cgccggaggc gagtaccacc tcgacctcga
     1921 cggtcgggtt cccgcgggag tcgaggacct cacggcccac gacgtggatg atctcgctca
     1981 tcgtcgtccg ctccttgcgg tgagaggacg gggagggtcg ggccggggtc gcgacgcgct
     2041 gtggctcacg tccgtgttgc tcctgtggct ggtgtggccc gtgttcccca gcccgtttct
     2101 agccgatcag ccggcggatt ccgcggcacg cagtgcgtcc cgccacgccg ccgcggtgcg
     2161 gcgcagggcg tcttcggggt cgatgcccgc gtcgcgggcc cgcgtcacga tggccagcag
     2221 ctcggcaccg aggtcgttgc cagcgggtgg agggacccgg tcgagcagcc cggtggccgc
     2281 ggccttcttc tcgatcttga gggcgaagag cagggcgggg aggccgcggg ggatgccgtc
     2341 gaagacgctg tcccgcccct tctcc
//
LOCUS       DASTFV010000671         4710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2543333, whole genome shotgun sequence.
ACCESSION   DASTFV010000671 DASTFV010000000
VERSION     DASTFV010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2543333"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VFP02_12315"
     CDS             complement(<1..268)
                     /locus_tag="VFP02_12315"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9543866.1"
                     /translation="MGSAAVSPGLVCAHHHLYSALARGMPAPPRTPTTFREILELVWW
                     RLDVALDLDTIAWSARLGALEALAVGCTAIVDHHESPNAIDGSLS"
     gene            complement(268..2802)
                     /locus_tag="VFP02_12320"
     CDS             complement(268..2802)
                     /locus_tag="VFP02_12320"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET9543867.1"
                     /translation="MTTFTLNGAAVEVPDDRAHLLVALREDLGIISAKDGCAPSGQCG
                     CCTVLLDGKAVVACQVSLEKVAGREVVTLEGIEERQRLADAFAAAGALQCGFCTPGIL
                     VRTKALLEKGDLTREKAARHLGAHLCRCTGYIKILDAIEALARGESPVPCLDGGVGAR
                     AGKHQGSELSAGSRPFIDDLCPEGLLHAVLRLADHARADVVHIDTTAAAAVPGVEAVL
                     TAADVPGELRVGLIHKDWPVFIPEGGRTSYLGDVLAVVVAADRATAERAAALVDVAYE
                     VLAPIVDPEAALAGDEDAVWGLDGNELSRSTYARGGDVDATLAASAHVVRQTFRTQRV
                     EQAFLEPESTLAAPRPDGVLQVWSGGQGVWDDRDQIASVLGLPIDAVEVELVSNGGAF
                     GGKEDMANQAQTALAAHLLQRPVKCTLTREQSLLIHPKRHPIAMRYEAGCDAEGRVTA
                     LRARLIGDSGPYASVGMKVLERAAGHAGGPYAWPAIDVESVAVRTNNPVCGAFRGFGA
                     NQAQFATEGVLDRLAELVGISGWEIRARNVIEPGALWGPGQRMDDGCLGARQCLDAVR
                     PAYDAAVAAGRPVGVGLGLKNAGLGNGLVEVAKAVVRFEEDGTIEVRHCWTDMGQGVD
                     TVARQVAVEELGVDASQIRVVVDTSRELGNGQTTGSRGTIMGAGSIADACAAALADGC
                     KPGIDYPGEYRVDWTNSLNDGVAEPVIHSTFGYAAQLVILDPETGEVTRVVAAHDVGR
                     AVNPVLCEGQIEGAVHMGLGYALSEDFPADDQGRPTNMTLRSLAIIRPKDMPPVEVIL
                     VESPQPNSPYGIKGVGEIGLVPTAGAVAAALHAHDGQWRSQLPMSV"
     gene            complement(2799..3683)
                     /locus_tag="VFP02_12325"
     CDS             complement(2799..3683)
                     /locus_tag="VFP02_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoyl-D-amino-acid hydrolase"
                     /protein_id="HET9543868.1"
                     /translation="MRVAGAQLGPIARDERRADVVERLIALLRAAADDGAELVAFPEL
                     ALTTFFPRWVLAGDELDAFYEIEMPGPETKPLFEEAARLGVGFALGYAELADGHRYNT
                     TVLVDGQGSVVGHYRKVHLPGHEEPEPWRPFQHLERAYFEPGDGFTTWSAFGAEVGLA
                     TCNDRRWPETYRVLALEGAEVVLIGYNTPIHYPPDPDQDRLAGFHNRLVMQAGAYQNG
                     LWVVGVAKGGTEEGVELLAESCIIAPSGEIVAEATTTGDEVIVADVDPAACAHYRGTL
                     FDFARYRRPEVYGKICEP"
     gene            complement(3680..>4710)
                     /locus_tag="VFP02_12330"
     CDS             complement(3680..>4710)
                     /locus_tag="VFP02_12330"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HET9543869.1"
                     /translation="REDLAPVMTGSHIDTVATGGRYDGNLGVLGGLEVVEALNDAGVV
                     TRRPIEVAFFTNEEGARFSPDMLGSMAYAGGLPLEEALDRTDPDGVVLGDELDRIGYR
                     GTMPVPGPVPHAFVELHIEQGPVLERRGHTIGAVTGVQGISWQELTIRGQANHAGTTP
                     MAMRHDAGFAAGAVTQRVRDLVTEMGGAQVGTVGVIDVDPGLTNVVARRARMTVDLRN
                     TDEALLAEAECRLADHVREVAEAEGVTVDRRATARFEPVTFDARVIDLVEATAQRLGH
                     SVQRLPAGAGHDAGLIAGVCPAGMVFTPSHEGISHNPAEHTDPEDLDAGADVLLQVLL
                     TLAEEDPP"
BASE COUNT          632 a         1714 c         1721 g          643 t
ORIGIN      
        1 ccgacagcga accgtcgatc gcgttgggcg actcgtggtg gtcgacgatc gccgtgcacc
       61 cgaccgccag tgcttccagc gcccccagcc gggccgacca ggcgatcgtg tcgaggtcga
      121 gggccacgtc cagccgccac cagaccagtt cgaggatctc ccggaacgtc gtgggcgtcc
      181 tcggcggcgc cggcatcccg cgggcgagcg ccgagtacag gtggtggtgg gcacagacca
      241 gcccgggcga gacggcggcg gaccccacta caccgacatc gggagctgcg aacgccactg
      301 gccgtcgtgg gcgtggaggg cggccgccac cgcgccggcc gtggggacga ggccgatctc
      361 gcccaccccc ttgatgccgt agggggagtt gggctggggc gactcgacca ggatcacctc
      421 gaccggcggc atgtccttgg gccggatgat ggccaggctg cgcagggtca tgttggtcgg
      481 gcggccctgg tcgtccgccg ggaagtcctc ggacagggcg tagccgaggc ccatgtgcac
      541 ggccccctcg atctggcctt cgcacaggac cgggttcacg gcccggccga cgtcatgggc
      601 ggccacgacc cgcgtcacct cgcccgtctc cgggtcgagg atcacgagct gggcggcgta
      661 gccgaacgtg gagtggatga ccggttcggc gacgccgtcg ttgagcgagt tcgtccagtc
      721 gacccggtac tccccggggt agtcgatgcc cggcttgcag ccgtcggcga gggcggcggc
      781 gcaggcatcg gcaatggagc cggcgcccat gatcgtcccc cggctgccgg tggtctgccc
      841 gttgccgagc tcgcggctgg tgtcgaccac gacgcggatc tgggacgcgt ccacccccag
      901 ctcctccacg gcgacctggc gggccacggt gtcgacgccc tggcccatgt ccgtccagca
      961 atggcgcacc tcgatcgtgc cgtcctcctc gaagcggacc acggccttgg ccacctcgac
     1021 gaggccgttg ccgagcccgg cgttcttcag gcccaggccc acgccgaccg gccggcccgc
     1081 cgcaaccgcg gcgtcgtacg cgggccggac cgcgtcgagg cactggcggg cgccgaggca
     1141 accgtcgtcc atgcgctggc cgggacccca gagggcgccc ggctcgatca cgttgcgggc
     1201 ccggatctcc cagcccgaga tgccgaccag ctcggccagc cggtcgagca cgccctcggt
     1261 ggcgaactgg gcctggttgg cgccgaagcc ccggaaggcg ccgcacacgg ggttgttggt
     1321 ccgcacggcc accgactcga catcgatggc cggccaggcg tacggcccgc cggcgtggcc
     1381 cgcggcccgc tcgaggacct tcatgcccac cgaggcgtag ggccccgagt caccgatcag
     1441 gcgggcccgg agggcggtca cccggccctc ggcgtcacag ccggcctcgt agcgcatggc
     1501 gatcggatgg cgcttggggt ggatcaacag cgactgctcg cgggtcagcg tgcacttgac
     1561 gggccgctgc aggaggtggg ccgccaacgc ggtctgggcc tggttggcca tgtcctcctt
     1621 gcccccgaac gcccctccgt tggagaccaa ctcgacctcg acggcatcga tcggcaggcc
     1681 gagcacggac gcgatctgat cgcggtcgtc ccaaacaccc tggccgccgg accacacctg
     1741 gagcacgccg tcggggcgag gcgcggcgag ggtggactcg ggctcgagga aggcctgctc
     1801 cacccgctgg gtccggaagg tctggcgcac gacgtgggcc gaggcggcca gcgtggcgtc
     1861 gacatcgccc ccccgggcat aggtcgaacg ggacagctcg ttgccgtcga ggccccacac
     1921 cgcgtcctcg tcgccggcca gggcggcctc ggggtcgacg atgggcgcca ggacctcgta
     1981 ggccacatcg accagggcgg ctgcgcgctc ggccgtcgcc cggtccgcgg ccacgaccac
     2041 ggccaagacg tcacccaggt acgacgtccg tccgccctcg gggatgaaga ccggccagtc
     2101 cttgtggatc aggcccaccc gcagctcacc cggcacgtcg gccgcggtca ggacggcctc
     2161 gacgccggga acggccgcgg cggccgtggt gtcgatgtgc acgacgtcgg cccgggcgtg
     2221 gtcagccaac cggagcacgg cgtgcaggag gccttcggga cacaggtcgt cgatgaacgg
     2281 gcggctgccg gcggacaact cgctgccctg gtgcttgcct gcccgcgccc cgacgccccc
     2341 gtcgaggcac ggcaccggcg actcaccccg ggccagggcc tcgatggcgt cgaggatctt
     2401 gatgtagccg gtgcagcggc agaggtgggc gccgaggtgg cgggccgcct tctcccgggt
     2461 caggtcgccc ttctccagca gcgccttcgt tcgcaccagg atcccgggcg tgcagaaccc
     2521 gcactggagc gcgccggcgg cggcgaaggc gtcggccaag cgctggcgct cctcgatgcc
     2581 ctccagggtg acgacctccc ggccggccac cttctccagc gacacttggc aggcgaccac
     2641 ggccttgccg tcgagcagca ccgtgcagca gccacactgc cccgaggggg cgcagccgtc
     2701 cttggccgag atgatcccga ggtcttcccg cagcgctacc agcaggtggg cccggtcgtc
     2761 gggcacctcg accgcggcgc cgttgagggt gaacgtggtc atggctcgca gatcttcccg
     2821 tagacctccg gccgccggta gcgggcgaag tcgaacagcg tcccccggta gtgggcgcag
     2881 gctgccgggt cgacgtcggc cacgatcacc tcgtcaccgg tcgtcgtcgc ctcggccacg
     2941 atctcgcccg agggggcgat gatgcagctc tccgccagga gctcgacacc ttcctccgtg
     3001 ccgcccttgg ccacgccgac gacccacagg ccgttctggt acgcgccggc ctgcatgacc
     3061 agccggttgt ggaacccggc cagccggtcc tggtctggat cgggcgggta gtggatgggg
     3121 gtgttgtacc cgatgagcac cacctcggcg ccctccaggg ccagtacccg gtaggtctcg
     3181 ggccagcgcc ggtcgttgca ggtggcaagg ccgacctcgg ccccgaaggc cgaccaggtg
     3241 gtgaacccgt cgccgggctc gaagtaggcc cgttcgaggt gctggaacgg tcgccacggc
     3301 tccggttcct cgtgcccggg caggtggacc ttgcggtagt ggccgacgac cgagccctga
     3361 ccgtcgacca gcacggtcgt gttgtagcga tgaccgtcgg ccagctccgc gtagccgagg
     3421 gcgaacccga caccgaggcg ggcggcctcc tcgaagaggg gcttggtctc gggaccgggc
     3481 atctcgatct cgtagaaggc atcgagctcg tcgcccgcca gcacccagcg ggggaagaag
     3541 gtggtcaggg ccagctcggg gaaggccacc agctcggccc cgtcgtcggc cgcggcccgg
     3601 aggagggcga tgaggcgctc gacgacgtcg gcccgccgtt cgtcccgggc gatcggtccg
     3661 agctgggcgc cggcgaccct cacggcgggt cctcctcggc cagcgtgagc aggacctgga
     3721 gaaggacgtc ggcgccggcg tcgaggtcct cggggtcggt gtgctcggcc gggttgtggc
     3781 tgatcccctc gtggctgggc gtgaagacca tgcccgccgg gcagacgccg gcgatcagcc
     3841 cggcgtcgtg acccgcaccg gcgggcagcc gctgcaccga gtggcccagg cgctgagccg
     3901 tcgcctccac caggtcgatg acccgggcgt cgaacgtcac cggctcgaag cgggccgtgg
     3961 cgcggcggtc gacggtgacg ccctcggcct cggccacctc gcggacgtga tcggccaggc
     4021 ggcactcggc ctcggcgagc agggcctcgt cggtgttgcg caggtccacg gtcatccggg
     4081 cccgccgggc gaccacgttg gtcagcccgg ggtcgacgtc gatgacgccg acggtaccca
     4141 cctgggcgcc gcccatctcc gtgaccaggt cgcgcacccg ctgggtcacg gcgccggcgg
     4201 cgaaaccggc gtcgtggcgc atcgccatcg gcgtcgtgcc ggcgtggttg gcctgtcccc
     4261 ggatggtcag ctcctgccag gagatgccct gcacgccggt gacggccccg atcgtgtggc
     4321 cccggcgctc caggaccggc ccctgctcga tgtggagctc gacgaaggcg tgggggaccg
     4381 ggccgggaac cggcatcgtt ccccggtagc cgatccggtc cagctcgtcg ccgagcacga
     4441 cgccgtcggg gtcggtgcgg tcgagggcct cctccaacgg caggccgccc gcgtaggcca
     4501 tgctccccag catgtcgggg ctgaaccggg cgccctcctc gttggtgaag aaggccacct
     4561 cgatcggtcg tcgggtgacg acgccggcgt cgttcagcgc ctcgaccacc tccagcccgc
     4621 cgagcacgcc gaggttgccg tcatagcgcc cgccggtggc cacggtgtcg atgtggctac
     4681 cggtcatcac cggggcgagg tcctcgcgcc
//
LOCUS       DASTFV010000672         4509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2553010, whole genome shotgun sequence.
ACCESSION   DASTFV010000672 DASTFV010000000
VERSION     DASTFV010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2553010"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFP02_12335"
     CDS             complement(<1..529)
                     /locus_tag="VFP02_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET9543870.1"
                     /translation="MEPVRNYLVPTVVEQSNRGERAYDLYSRLLKENIIFLGTPIDDT
                     IANLVCAQLLHLESENPDKDINIYINSPGGDITALFAIYDTMQYIKPDIATICYGQAA
                     SAAAVLLAAGAPGKRLALPHSRILLHQPYAGAQGQATDIELAAKEILRLRELLEEILA
                     EHTGQPIERLHNDTDR"
     gene            complement(529..1938)
                     /gene="tig"
                     /locus_tag="VFP02_12340"
     CDS             complement(529..1938)
                     /gene="tig"
                     /locus_tag="VFP02_12340"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET9543871.1"
                     /translation="MKATVETLEGNKVKVSVEVDETEFESAVDDAFRRIAREVRIPGF
                     RPGKAPRRVLEAQLGTGFAREEALRHALPEYYAQAVRENDVDVIAPPEIDITGGEEDG
                     PVVFDAVVEIRPEIAIRGHDDLTVTIPSPLVSDEEVDAQVERLRQNFAELATVDRAAQ
                     DGDNVTIDIAGWEHDEPVSGLTADDYLYEVGAGAVVAEIDDQLRGAKVGDILEFDAGH
                     PDPDSEASLRIRIMVKEIKEKVLPEVDDEWANEASEFETVEALREDLRKRSAMTRVIM
                     AQMGLRNGAAQAAGDLVDADDVPEAMVSAEMEHRLQDLAMRLQAQGMDFDSYMAASGQ
                     DQEGMVAELRDAAVMATKADLALRGIAEAEGLDATEEDLDEELEKLAERVEQSSAEVR
                     LALEEGDQIPAVRSDLRKRKALDWLVEHVQIVDEDGTPVDRADLEAPEVEEPEDDQSE
                     AEIEIEAAVEADENLEEQG"
     gene            2006..3433
                     /locus_tag="VFP02_12345"
     CDS             2006..3433
                     /locus_tag="VFP02_12345"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HET9543872.1"
                     /translation="MVFNSLQYLVFFPLVFAVYWQLKRRGQNVLLLLASWFFYAMWDW
                     RFLGLMLLTTGVDFGVGRYLAMTEDEKKRKLAFGLSLGLNLTVLGFFKYFNFFVGSAA
                     DLLQTFGLDASEPTLRVLLPVGISFYTFHGISYTFDVFRRDIEPADDLLDFAVFVAFF
                     PQLVAGPIGRAHLQLPQFEHERPPLRWEQARSGLCLILLGLFKKVAIADALAPFVDRT
                     FTEPGRASWISLLGGAYAFALQIYGDFAGYSDVARGSSRLLGIELPLNFEQPYLSRNI
                     TVFWRTWHISLSNWLRDYLYIPLGGNRGSRAKTYRNLMLTMVLGGLWHGAAWTFVAWG
                     TLNGLYLCAHRWFTERPGGAGDAHNGIGRFTWRDVLPAIGTFQLVVFAWIFFRSASFA
                     EAFDVITGILTLQGGVTEWGSLFLVGALGIVTIAIDIAQRNTDTHTPMLDLPPALQGA
                     LYGAFVVAIVLFSGGATVPFIYFQF"
     gene            3530..>4509
                     /locus_tag="VFP02_12350"
     CDS             3530..>4509
                     /locus_tag="VFP02_12350"
                     /inference="COORDINATES: protein motif:HMM:NF019145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543873.1"
                     /translation="MTRAVKFLAVLAAVVVAATAVVVPVTLRLLRDDAARAADLEDDL
                     QGRSDAPLTLTRTANGAAVRSVGTRAGSPIRVGGTDPAGTFLDRYGRLLGADRADLVP
                     IGDEPLLGGGTATRYQQTIDDVPVLGGELSVQVDADGGVLSSLADLSRGEPGIDTTPT
                     VRPVAARRAAVAATARAVEADPSTLSASAPEPWIYDPSVIGPPSASAPRLTWRVEVTS
                     TSDPVRQLVLVDAEEGTIALSFSQLAPGLNRKVCDAANTRQAPACSAPYARVEGQPRL
                     SLITSPATANEVDLAYDYSGATYNFFASRFDRDGIADDGAPILAKVRFCKT"
BASE COUNT          580 a         1648 c         1495 g          786 t
ORIGIN      
        1 cccggtcggt gtcgttgtgc agacgctcga tcggctgacc ggtgtgttcg gccaggatct
       61 cctccaggag ctcccgcagg cgcaggatct ccttggccgc cagctcgatg tcggtggcct
      121 ggccttgggc cccggcgtag ggctggtgga gcaggatccg ggagtgcggg agggccaggc
      181 gcttgcccgg agcccccgcg gcgagcagga cggccgcggc cgacgcggcc tggccgtagc
      241 agatggtggc gatgtcgggc ttgatgtact gcatcgtgtc gtagatggcg aacagcgccg
      301 tgatgtcacc gccggggctg ttgatgtaga tgttgatgtc cttgtccggg ttctcggact
      361 cgaggtgcag gagctgggcg cagaccaggt tggcgatcgt gtcgtcgatc ggcgtgccca
      421 ggaagatgat gttctccttg agcaggcggg agtagaggtc ataggcccgc tcaccccggt
      481 tgctctgctc gaccacggtc gggacgaggt agttgcgtac cggttccatc acccctgctc
      541 ctctaggttc tcgtcggctt cgactgctgc ttcgatctcg atctccgctt cgctctggtc
      601 gtcctccggc tcctcgacct ccggggcctc gaggtcggcc cggtcgaccg gggtcccgtc
      661 ctcatcgacg atctggacgt gctccacgag ccagtccagg gccttgcgct tgcgcaggtc
      721 cgatcgtacc gccgggatct ggtcgccctc ctcgagggcc agacggacct cggccgaaga
      781 ctgctcgacc cgctcggcca gcttctccag ctcctcgtcg agatcctcct ccgtggcgtc
      841 gaggccctcg gcctcggcga tgccgcgaag ggcgagatcg gccttggtgg ccatgacggc
      901 ggcgtcccgc agctcggcga ccatgccctc ttggtcctgg ccgctggccg ccatgtagct
      961 gtcgaagtcc atgccctggg cctggaggcg catggccagg tcctgcaggc ggtgctccat
     1021 ctcggccgag accatcgcct cggggacgtc gtcggcgtcg accaggtcgc cggcggcctg
     1081 ggccgcaccg ttgcgcagac ccatctgggc catgatcacc cgggtcatgg cggaccgctt
     1141 gcgcaggtcc tcacgcagcg cctcgacggt ctcgaactcg gaggcctcgt tggcccactc
     1201 gtcgtcgacc tcggggagca ccttctcctt gatctccttg accatgatcc ggatgcgcag
     1261 cgaggcctcg ctgtccgggt ccgggtgccc ggcgtcgaac tcgaggatgt cgccgacctt
     1321 cgccccgcgg agctggtcgt cgatctcggc caccacggcc ccggcgccca cctcgtagag
     1381 gtagtcgtcg gcggtgaggc cggacacggg ctcgtcgtgc tcccagccgg cgatgtcgat
     1441 ggtgacgttg tcgccgtcct gggcggcccg gtcgaccgtg gccagctcgg cgaagttctg
     1501 acgcaggcgc tcgacctggg cgtcgacctc ctcgtcggag acgaggggcg acgggatggt
     1561 gaccgtgagg tcgtcgtggc cgcggatggc gatctcgggg cggatctcga cgacggcgtc
     1621 gaagacgacg gggccgtcct cctcaccacc ggtgatgtcg atctcgggcg gggcgatgac
     1681 gtcgacgtcg ttctcccgca ccgcctgggc gtagtactcc gggagggcgt ggcgcagggc
     1741 ctcctcccgg gcgaagccgg tgcccagctg ggcctccagc acgcggcggg gggccttgcc
     1801 ggggcggaac ccggggatgc gcacctctcg ggcgatgcgt cggaacgcat cgtccaccgc
     1861 ggactcgaac tcggtctcgt cgacctcgac cgagaccttg accttgttgc cctcgagggt
     1921 ctcgaccgtg gccttcatgg agcggggagt gtaccggcgc gcccgcgagg acctcaggga
     1981 cggtcattag gctccggccc cggtcatggt cttcaactcc ctgcagtacc tcgtcttctt
     2041 cccgctcgtc ttcgccgtct actggcagct gaagcggcgc ggccagaacg tgctgctgct
     2101 cctggcctcg tggttcttct acgccatgtg ggactggcgc ttcctcggcc tgatgctcct
     2161 gacgaccggg gtggacttcg gggtcggccg ctacctcgcc atgaccgagg acgagaagaa
     2221 gcggaagctg gccttcggcc tgagcctcgg tctcaacctg acggtgctcg gcttcttcaa
     2281 gtacttcaac ttcttcgtcg gctcggccgc cgacctgctc cagaccttcg ggctcgacgc
     2341 cagcgagccg accctgcggg tcctgttgcc ggtcgggatc agcttctaca cgttccacgg
     2401 gatcagctac acgttcgacg tcttccgccg cgacatcgag cccgccgacg acctcctcga
     2461 cttcgccgtg ttcgtcgcct tcttcccgca gctggtggcc gggcccatcg gccgggccca
     2521 cctccagctc ccccagttcg agcacgagcg gccgcccctg cgctgggagc aagctcggtc
     2581 cgggctctgc ctgatcctcc tcggcctgtt caagaaggtc gccatcgccg acgcgctggc
     2641 ccccttcgtc gaccgcacgt tcaccgagcc cggacgagcc tcctggatct ccttgctcgg
     2701 tggggcctac gcgttcgccc tccagatcta cggcgacttc gccgggtact ccgacgtcgc
     2761 ccgcgggtcc tcccgcctcc tcggcatcga gttgcccctg aacttcgagc agccctacct
     2821 gtcccggaac atcaccgtgt tctggcggac ctggcacatc tcgctgtcca actggctacg
     2881 ggactacttg tacatccccc tcggggggaa ccggggctca cgggcgaaga cctaccgcaa
     2941 cctgatgctg accatggtgc tgggcggact ctggcacggg gcggcgtgga ccttcgtggc
     3001 ctgggggacg ctgaacggct tgtacctctg cgcccaccga tggttcaccg aacgaccggg
     3061 aggggcgggc gacgcccaca acggcatcgg ccgtttcacc tggcgcgacg tgctcccggc
     3121 catcggcacc ttccagctcg tcgtgttcgc ctggatcttc ttccggtcgg ccagcttcgc
     3181 cgaggcgttc gacgtgatca ccgggatcct gaccctgcag ggtggggtca ccgagtgggg
     3241 atcgctcttc ctggtcggcg cgctcgggat cgtcaccatc gccatcgaca tcgcccaacg
     3301 gaacaccgac acccacacgc cgatgctcga cctcccaccc gccctccagg gcgcgctcta
     3361 cggcgccttc gtggtggcca tcgtcctgtt ctcgggcggg gccaccgtcc ccttcatcta
     3421 cttccagttc taggcccgat cgtggacgct ccgcgtcgac catggtgacg ctcagcgaga
     3481 tcacgcgcgg cgcgtgacgc gccgcgctcc gtggccgtaa gctgggcggg tgacgagggc
     3541 cgtgaagttc ctcgccgtcc tcgcggcggt agtcgtggcc gccaccgccg tggtcgtgcc
     3601 cgtcaccctg cgtctgctgc gcgacgacgc cgcgcgggcc gccgatctcg aggacgacct
     3661 ccagggccgg tccgacgccc ccctcaccct cacccggacg gcgaacggcg ccgccgtccg
     3721 gtcggtgggc acccgcgcgg ggagcccgat ccgagtcgga ggcaccgacc ccgcggggac
     3781 gttcctcgac cgctacggcc gcctcctcgg cgccgaccga gccgacctgg tcccgatcgg
     3841 tgacgagccc ttgctgggcg gcggcaccgc gacccggtac cagcagacga tcgacgacgt
     3901 ccccgtgctg ggcggcgagc tcagcgtgca ggtcgacgcc gacggcgggg tcctctccag
     3961 cctggccgac ctgtcccgcg gggaaccggg catcgacacg acgcccaccg tccgcccggt
     4021 cgccgcccgc cgggccgccg tcgccgctac ggcgagagcc gtcgaggccg acccctcgac
     4081 gctcagtgcc tcagcccccg agccgtggat ctacgacccg agcgtcatcg gtccgccgtc
     4141 cgcctcggcc cctcgcctga cctggcgggt cgaggtcacc tcgacgagcg accccgtccg
     4201 tcaactcgtg ctggtcgacg ccgaggaagg cacgatcgcc ctgtcgttca gccagctggc
     4261 ccccggcctc aaccgcaagg tgtgtgacgc ggccaacacc cgccaggccc cggcctgcag
     4321 cgcgccgtac gcccgggtag agggccagcc ccgcctcagc ctcatcacca gcccggccac
     4381 ggcgaacgag gtcgatctgg cctacgacta ctcgggcgcc acctacaact tcttcgcctc
     4441 ccgcttcgat cgggacggca tcgccgacga cggggcgccg atcctggcca aggtgcggtt
     4501 ctgcaagac
//
LOCUS       DASTFV010000673         5821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2553343, whole genome shotgun sequence.
ACCESSION   DASTFV010000673 DASTFV010000000
VERSION     DASTFV010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2553343"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..579)
                     /locus_tag="VFP02_12355"
     CDS             complement(109..579)
                     /locus_tag="VFP02_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9543874.1"
                     /translation="MDVSVTVNGAVATDDVEPRLLLVHYLRDVRGLTGTNVGCDTSSC
                     GACTVLVNDESVKSCTMLAVQADGQEITTIEGLANGTELHPMQQAFHENHALQCGYCT
                     PGMVMAAVSLLEEHDDLDEAGVREGLEGNLCRCTGYHNIVKAVMAAAGAEGGPA"
     gene            complement(661..1410)
                     /locus_tag="VFP02_12360"
     CDS             complement(661..1410)
                     /locus_tag="VFP02_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9543875.1"
                     /translation="MDLGLRGKRAAVTAGSAGLGLGCAKALAAEGVQVSLCGRDRAKA
                     EAAAADIGAVALMADMSDVDQATGFIEQASERLGGTIDIVVANAGGPPAGTFSSTDLN
                     GYRQALELNLLSSIALCRAAVPAMQAQRWGRVVAITSIGVRQPPPYLMASATARAGLT
                     AFLKVLATEVAPDGVTVNSAQPGMHDTDRVRQLHGGGTPPAEAAAGIPVGHLGDAEDF
                     GAVVAFLCSQQARFVTGTSLLVDGGAFPGLV"
     gene            1446..2333
                     /locus_tag="VFP02_12365"
     CDS             1446..2333
                     /locus_tag="VFP02_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HET9543876.1"
                     /translation="MRIVSLLPSATEIVFALGLGDDLAGVTFECDFPPEARGKRIISS
                     SGLEASADDPEAIDAAVRAATEGGVPLYRLDEAAIAEIDPDVILTQDLCRVCAVPSGD
                     VDEALDRLGCSAVVISLDPTSLDEVIDDIGRVGTVLDVTDRGAELTAALRLELGVIRS
                     VLADRPRPRVLVLEWIDPPFGAGHWIPDMVRAAGGEPVLATDGAASGPLTWEAVVDAQ
                     PDVAVIAPCGFGLEGAVRHARQARPHLPSTAAVWAVDGDAYIVRPGPRLVDGVKVLAE
                     ILHPEVVGSSSPEAAALVT"
     gene            2338..3243
                     /locus_tag="VFP02_12370"
     CDS             2338..3243
                     /locus_tag="VFP02_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9543877.1"
                     /translation="MGTTPVEVADALAAHDYLADDGLATAIFLALELQRPLLLEGEAG
                     VGKTEVAKVLARWTEGELIRLQCYEGIDVAQAVYEWDYARQLLHLRTAEATGRAEGAA
                     ADALEDELYTERFLVRRALLQAIDHADGPPPVLLIDEVDRADDEFEAFLLEILSDYQV
                     TVPELGTFSTPNPPVVIITSNRTRDVHDALKRRCLYHWVEHPDFDREVAIVRVRAPRV
                     AESLARQVAGVVEQLRELNLYKPPGVAETIDWAQALAALGKADLDEETVGVTLGTVVK
                     YREDQQRVRDQGIGDLIRKAVLRGA"
     gene            3257..4363
                     /locus_tag="VFP02_12375"
     CDS             3257..4363
                     /locus_tag="VFP02_12375"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9543878.1"
                     /translation="MRPERAAVAFGRVLRSAELEVPVDSVLAFVRAWEAVGSDDRRLV
                     YWAGRATLVRQPEDIGVYDVAFAAFFGGNQDLGPGPPPLAPPPVPVPTATDDGGDGDD
                     DGPEEDRPAQVVRWSPGEVLRHKDFAACTDAERTEAMRLLAQLKVRRARRPSRRRRPT
                     RQPGRWPDLRRTTRAAMRSGGETIDRRWLDPGERPRRLVLLVDVSGSMEAHARALLRF
                     AQVVVAGGTRVEAFAIGTRLTRLTRELSSRDPDAAMRAASSAVVDWSGGTRLGACLRE
                     FNDDWGVRGLARGATVVVLSDGWDRGEPELLGAEVERLHRITHRLIWVNPLKASAGYE
                     PLARGMAAALPHVDQFLEGHSLASLEDLAEVISA"
     gene            4360..4674
                     /locus_tag="VFP02_12380"
     CDS             4360..4674
                     /locus_tag="VFP02_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET9543879.1"
                     /translation="MKDLLSDLDRWRAAGKKVALARVVDVEGSGPRGPGAAMAVSEDS
                     EVAGSVSGGCVEGAVVTEALDILATGERRVVTFGYSDDEAFAVGLTCGGTVHLFIEPM
                     DW"
     gene            4677..5456
                     /locus_tag="VFP02_12385"
     CDS             4677..5456
                     /locus_tag="VFP02_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HET9543880.1"
                     /translation="MSLYDELRNALRDDRPVALATIIDGPGIGQKLLVGPDRESVGTL
                     GNEDLDRVVSRDALGQLLAGRTDRRNYGARGEARETVVDVFIEAFAPAPRMLIFGAVD
                     FTAALATAAKFLGFHVTVCDAREVFATVRRFPMADEVLVDWPHRLVEREGDRLGPADA
                     ICVLTHDNKFDVPAIVAALATDVGYLGAMGSRRTHAKRVERLKEEGVTDAQLERVMAP
                     IGLDIGSRTPEETAISVCAEIIANHTGHTAGSLKVTEGPIH"
     gene            complement(5457..>5821)
                     /locus_tag="VFP02_12390"
     CDS             complement(5457..>5821)
                     /locus_tag="VFP02_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543881.1"
                     /translation="ASVVAVDTEDGSVVTLDALRTNEQITSWAAFSPVGPWALVATDA
                     PEQDDQPVTRGRLVNLDTGEVVDLGTGFQGGTFSPDGRQVAWSSGDAAELRIAPVDDL
                     ATAEVVTTGVAVPVWLAA"
BASE COUNT          801 a         2081 c         2091 g          848 t
ORIGIN      
        1 cgatgcccag ccagagggcg ccggtgatgg ggaggtcgtc gacgtagcgg gcctcgccgg
       61 acagcagctc ggggtcctcc ttgcggagca tccgggtacc ggtgacggtc atgccggacc
      121 cccctccgcg ccggcggcgg ccatgaccgc tttgacgatg ttgtggtagc cggtgcagcg
      181 acagaggttg ccctcgaggc cctcccgcac gccggcctcg tcgaggtcgt cgtgctcttc
      241 gaggagggag acggcggcca tgaccatgcc cggcgtgcag tacccgcact gcaaagcatg
      301 gttctcgtgg aaggcctgct gcatcggatg gagctcggtg ccgttggcca ggccctcgat
      361 ggtggtgatc tcctggccgt cggcctggac ggccagcatc gtgcaggact tcacggattc
      421 gtcgttgacg agcacggtgc aggccccaca cgacgaggtg tcacagccga cgttggtgcc
      481 ggtgaggccg cggacgtcgc gcaggtagtg gaccagcagc aaccgtggct cgacgtcatc
      541 agtcgccacc gcgccattga cggtcacgga cacatccatg cgtcaccccc cggtgaaggt
      601 cgtccggcca tcctcgcgcg agattccggc ttgtgctgat cgtcgcgtca gtaggcgcgg
      661 tcagaccagg cccggaaagg cgccgccgtc gaccaggagc gaggtgccgg tcacgaagcg
      721 ggcctgctgg gagcagagga aggcgaccac ggcgccgaag tcttcggcat cgcccaggtg
      781 accgacgggg atgcccgcgg ccgcctccgc aggcggtgtt ccgccaccat ggagctggcg
      841 gacccggtcg gtgtcgtgca tcccgggctg ggccgagttg acggtcaccc cgtcgggggc
      901 gacctccgtg gccaggacct tcagaaaggc cgtgagcccg gcgcgggctg tggccgatgc
      961 catgaggtag ggcggcggct ggcggacccc gatggaggtg atcgccacca ctcgacccca
     1021 gcgctgcgcc tgcatggcgg gcacggcggc ccgacacagg gcgatcgacg acagcaggtt
     1081 cagctcgagg gcctgtcgat acccgttgag gtcggtcgac gagaaggtcc cggccggtgg
     1141 cccgccggcg ttggccacga cgatgtcgat cgtccctccg agccgttcgc tcgcttgctc
     1201 gatgaagccc gtggcctggt cgacgtcgct catgtcggcc atgagggcga cggcaccgat
     1261 gtccgccgcg gccgcttcgg ccttggcccg gtcccgcccg cagagggaca cctgcacgcc
     1321 ctcggcggcg agcgccttcg cgcagccgag gcccaagccc gccgatcctg ccgtcactgc
     1381 agcccgcttc ccacgcagtc cgagatccat gggccgaacc taggtcgccg ccgccacact
     1441 gcggtatgcg gatcgtgtcg ctcctgcctt cagccacgga gatcgtgttc gcgttggggc
     1501 tcggtgacga cctggcgggg gtgacgttcg agtgtgactt cccgcccgag gcgcggggga
     1561 agcggatcat ctcctcgtcc gggttggagg cgagcgcgga tgacccggag gcgatcgacg
     1621 ccgccgtgcg ggcggcgacg gagggcgggg tgccgctgta ccggttggat gaggcggcga
     1681 tcgccgagat cgatcccgac gtgatcctga cccaggacct gtgccgggtc tgcgccgtgc
     1741 cgtcggggga cgtcgacgag gccctcgacc gcctcgggtg ctcggcggtg gtgatctcac
     1801 tcgaccccac gtcgctcgat gaggtgatcg acgacatcgg tcgggtcggc accgtcctgg
     1861 acgtgaccga tcgcggcgcc gagctcacgg ccgccctccg gctcgagctg ggcgtcatcc
     1921 gcagcgtgct cgccgaccgg ccccgacctc gggtcctggt cctcgagtgg atcgatccgc
     1981 ctttcggcgc cgggcactgg ataccggaca tggtgcgggc ggccggcggc gagccggtcc
     2041 tcgctactga cggcgcggcc tccgggccgc tcacgtggga ggcggtggtc gacgcccagc
     2101 ccgacgtcgc ggtgatcgcc ccgtgcggat tcgggctgga aggcgccgtc cggcacgccc
     2161 ggcaggcgcg gccgcacctg ccctccacgg ccgccgtctg ggccgtggac ggcgacgcct
     2221 acatcgttcg accgggaccc cgtctggtcg atggcgtcaa ggtcctggcc gagatcctgc
     2281 accctgaggt ggtgggctcg tcgtcgccgg aggcggccgc cctcgtaacc tgaccctgtg
     2341 ggaacgactc cggtcgaggt ggcggacgcg ctcgcggcgc acgactacct ggctgacgac
     2401 ggcctggcca cggccatctt cctggctctg gagctgcagc gaccactgct tctcgaaggc
     2461 gaggccgggg tgggcaagac cgaggtggcc aaggtcctgg cccgctggac ggagggtgag
     2521 ctcatccgcc tccagtgtta cgagggcatc gacgtcgccc aggccgtgta cgagtgggac
     2581 tacgcccgac agctcctcca cctgcgcacg gcggaggcca cgggccgggc cgagggcgcg
     2641 gccgccgacg ctctcgagga cgagctctac accgagcgat tcctcgtccg tcgagccctt
     2701 ctgcaggcca tcgaccacgc cgacggcccg ccgccggtcc tcctgatcga cgaggtggac
     2761 cgggccgatg acgagttcga ggccttcctg ctcgagatcc tctccgacta ccaggtcacg
     2821 gttcccgagc tgggcacctt ctcgacgccg aacccgccgg tcgtgatcat cacgtcgaac
     2881 cggacccgag acgtgcacga cgccctcaaa cgccgctgcc tctaccactg ggtcgagcac
     2941 cccgacttcg accgcgaggt cgccatcgtc cgggtccggg cgccccgggt ggctgagagc
     3001 ctggctcggc aggtcgccgg cgtggtcgaa cagctccgcg agctcaacct ctacaagccc
     3061 cccggcgtgg ccgagacgat tgactgggcc caggccctcg ccgcgctggg caaggccgac
     3121 ctcgacgagg aaacggtcgg cgtcaccctc ggcaccgtcg tcaagtaccg cgaagaccag
     3181 cagcgggtcc gggaccaggg catcggggac ctcatccgga aggccgtcct gcgtggtgcc
     3241 tgagacaaca tcgcccttgc gtcccgaacg ggcggcggtg gcgttcggcc gggtcctgcg
     3301 ctcggccgag ctggaggtgc cggtcgacag cgtgctcgcc ttcgtgcggg cctgggaggc
     3361 cgtgggctcc gacgaccgcc gcctcgtcta ctgggccgga cgagccacgc tggtccgaca
     3421 gcctgaggac atcggcgtct atgacgtggc cttcgccgcg ttcttcggtg gcaatcagga
     3481 cctggggccc ggcccacctc cgctcgcgcc cccgcccgta cccgtgccga cagcgaccga
     3541 cgacggcggt gacggtgacg acgacggtcc cgaagaggac cgcccggccc aggtcgtgcg
     3601 ttggtcaccg ggcgaggtcc ttcgccacaa ggacttcgcg gcgtgcacgg atgccgagcg
     3661 aaccgaggcc atgcgcctct tggcccagct caaggtgcgc cgcgcccggc gtccctcccg
     3721 ccgccgccgg cccacccgac agccggggcg ctggccggac ctgcgccgga ccacccgggc
     3781 cgcgatgcgc tccgggggcg agacaatcga ccggcgctgg ctggacccgg gcgagcggcc
     3841 ccgccgcctc gtcctcctgg tggacgtgtc gggctcgatg gaggcccacg cccgggccct
     3901 gctccgcttc gcccaggtcg tcgtggcggg ggggacgagg gtcgaggcct ttgccatcgg
     3961 cacgcgcctg acccgcctga cccgggagct gtcgtcccgg gaccccgacg ccgccatgcg
     4021 ggcggcgagc tccgccgtcg tcgactggtc gggtgggacc cggctcggtg cctgcctgcg
     4081 ggagttcaac gacgattggg gcgtccgcgg gctggcgcga ggcgccactg tggttgtcct
     4141 ctccgacggg tgggaccgag gcgagcccga gctgctcggg gccgaggtcg agcgcctgca
     4201 ccggatcacg caccgcctca tctgggtcaa ccccctcaag gccagcgccg ggtacgagcc
     4261 ccttgcccgc gggatggctg cggccctacc ccacgtcgac cagttcctcg aaggccactc
     4321 gctggcctcg ctcgaggacc tcgcggaggt gatcagcgca tgaaggacct gttgtccgat
     4381 cttgaccgct ggagggcggc cgggaagaag gtggcgctgg cccgggtggt cgacgtcgag
     4441 ggctccggcc cgcgcggtcc gggtgcggcc atggcggtca gtgaggacag cgaggtagcc
     4501 ggctcggtct caggcggctg cgtcgagggc gccgtggtca ccgaggcact ggacatcctg
     4561 gccaccggcg aacgccgggt ggtgaccttc ggctattccg acgacgaggc gttcgccgtc
     4621 gggctcacct gcgggggcac ggtccacctc ttcatcgaac cgatggactg gtgacggtga
     4681 gcctctacga cgagctccgc aacgcgctcc gggacgaccg gccggtggcc ctggccacga
     4741 tcatcgatgg cccgggcatc ggccagaagc tcttggtcgg gcccgaccgg gagtcggtcg
     4801 gaaccctcgg caacgaggac ctggaccggg tcgtgagccg ggatgcgctc ggtcagctgc
     4861 tggccggccg caccgaccgt cgcaactatg gcgcccgggg tgaggcccgc gagaccgtgg
     4921 tcgacgtgtt catcgaagcg ttcgcccccg ccccccgcat gctcatcttc ggcgccgtcg
     4981 acttcacggc ggcgctggcc acggccgcca agttcctcgg cttccacgtc accgtgtgcg
     5041 acgcgcggga ggtcttcgcc accgtccgcc gcttcccgat ggccgacgag gtgctggtcg
     5101 actggcccca ccgcctggtc gagcgggagg gggaccggtt gggcccggcc gacgccatct
     5161 gtgtcctcac ccatgacaac aagttcgacg tcccggcgat cgtggccgcc ctggccaccg
     5221 atgtcggcta cctcggagcc atgggctccc gccgtaccca cgccaagcgg gtcgagcgcc
     5281 tgaaggagga aggggtcacc gacgcccagc tcgagcgggt catggcgccg atcggcctcg
     5341 acatcggctc ccgcacgccg gaggagacgg cgatctcggt ctgcgccgag atcatcgcca
     5401 accacaccgg ccacaccgcc ggctccctca aggtcaccga gggccccatc cactagtcag
     5461 gcggcgagcc agacgggaac ggcgacgccg gtcgtgacca cctcggcggt cgcgaggtcg
     5521 tcgaccgggg cgatccgcag ctcggcggcg tcaccgctgg accacgccac ctgccgaccg
     5581 tcgggcgaga acgtcccgcc ctggaacccg gtcccgaggt cgacgacctc gcccgtgtcc
     5641 aggttgacca gccggccgcg ggtgacgggc tgatcgtcct gctcgggggc atcggtggcg
     5701 accaaggccc agggaccgac cggcgagaac gccgcccaac tggtgatctg ctcgttggtt
     5761 cgcagggcgt ccagggtcac gacggagccg tcctcggtgt ccaccgccac gaccgaggct
     5821 c
//
LOCUS       DASTFV010000674         2659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2556292, whole genome shotgun sequence.
ACCESSION   DASTFV010000674 DASTFV010000000
VERSION     DASTFV010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2556292"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="VFP02_12395"
     CDS             complement(<1..1089)
                     /locus_tag="VFP02_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9543882.1"
                     /translation="MSINGVQDRDTLWIGGDWVSSDGGGSIEVISPHTEQVIATVPEA
                     STADVDNGVAAARAALESGPWATMGPAGRAEILTNLTGALGGRAEDLAQTITAEMGSP
                     IMFSHLGQVGATLMVADYYANLAKDFTFEEERQGLMSSVLVRREPVGVVGAIVPWNVP
                     LYVTMLKLAPALAAGATMVLKPAPESPLSAYLLAEALEEVGLPKGVVNVLPAGREVGE
                     HLVRHPGVDKIAFTGSTAAGMKIASICGEQLKRYTLELGGKSAAIILDDADVDTTLDN
                     LLQVAGLMNNGQACVAQTRVLASRAKYDEVVSGLASRMTAVKVGDPAEMDTEVGPLVA
                     ERQRDRVEKYIVQGQEEGAEVVVGGGRPA"
     gene            1189..>2659
                     /locus_tag="VFP02_12400"
     CDS             1189..>2659
                     /locus_tag="VFP02_12400"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HET9543883.1"
                     /translation="MANRPFTFADTFEVVADTVPDQTAIVTEDRRLTYAEVDERATRL
                     AHVLAERGVGAGDHVGLFLYNGTEYIEAMLAAWKIRAVPINVNYRYVEEELVYLFDDA
                     ELAAVIHHQEFTPTLAAVVDQVPGVHTFLYVADGSGVEMAGLPTEDYATALAAASTER
                     DFGERSGDDLYILYTGGTTGMPKGVMWRQEDIFFAAMDLSAMGLAEPPAAPEDLAARV
                     EESAGTLTMATAAPLMHGAAQWAAFICFHQGNTLVLAASRKFDPEGIWRMVEQEKVTT
                     MTVVGDAMARPLADELLANPDKYDLSSFFALGSGGAILSAGVKEQFTRARPDLIITDA
                     FGASETGANGGGVGSSDEGHPRFAVNDSTAVLDEDHRPLEPGSGVKGMLARTGRIPLG
                     YYKDEEKTARTFVHDADGKRWVIPGDFATVEADGTITLFGRGSVSINSGGEKIFPEEV
                     EAALKSHPAVFDAIVVGVPDERFGQRVAAVVAPRPGTSPS"
BASE COUNT          394 a          966 c          901 g          398 t
ORIGIN      
        1 ggcggggcga ccgccgccca cgacgacctc cgcaccctcc tcctggccct gcacgatgta
       61 cttctcgacc cggtcccgct gacgctcggc gacgagggga ccgacctcgg tgtccatctc
      121 ggccgggtcc ccgaccttga ccgcggtcat gcgactggcc aggccgctga cgacctcgtc
      181 gtacttggcc cggctggcca gcacccgggt ctgggcgacg caggcctgac cgttgttcat
      241 caggccggcg acctgcagca ggttgtccag cgtggtgtcg acgtcggcgt cgtcgaggat
      301 gatcgccgcc gacttgccgc ccagctcgag ggtgtagcgc ttgagctgct cgccgcagat
      361 ggaggcgatc ttcatgcccg ccgcggtgga gccggtgaag gcgatcttgt ccacgccggg
      421 gtgacggacc aggtgctcgc ccacctcacg gccggcggga aggacgttca cgacgccctt
      481 gggcaggccg acctcctcca gcgcctcggc caggaggtag gcgctgagcg gggactcggg
      541 ggccggcttg aggaccatgg tggcgccggc ggccagcgcc ggggccagct tcagcatggt
      601 cacgtagagc gggacgttcc aggggacgat ggcgccgacg acacccacgg gctcgcgccg
      661 cacgagcacg ctggacatga ggccctggcg ctcctcctcg aaggtgaagt ccttcgccag
      721 gttggcgtag tagtcggcca ccatcagggt ggcgccgacc tggcccaggt ggctgaacat
      781 gatgggcgag cccatctcgg cggtgatggt ctgggcgagg tcctcggccc ggccgcccag
      841 cgccccggtc aggttggtca ggatctcggc ccgaccggcg gggcccatgg tggcccaggg
      901 cccgctctcc agagcggccc gagcggcggc cacgccgttg tcgacatcgg ccgtggaggc
      961 ctcggggacg gtggcgatga cctgctcggt gtgcggggag atgacctcga tcgaaccgcc
     1021 gccgtcggag gagacccagt ccccaccgat ccagagggtg tcgcggtcct gtacgccgtt
     1081 gatgctcacc agttcccctt tgtgtcagcg gtcatgccgc tccacgcgaa catctgacac
     1141 cctgtcagat tcgggccgtt cggcaaagcc gtcctagggt cccgggccat ggcgaaccgc
     1201 cccttcacct ttgctgacac gttcgaggtc gtggccgaca cggtgcccga ccagaccgcc
     1261 atcgtcaccg aggatcgccg cctcacgtac gccgaagtgg acgaacgggc gacccgactg
     1321 gcccacgtgc tggccgagcg gggggtcggt gccggcgacc acgtcggcct cttcctctac
     1381 aacggcaccg agtacatcga ggccatgttg gcggcgtgga agatccgggc ggtcccgatc
     1441 aacgtgaact accgctacgt cgaggaggag ctggtctacc tcttcgacga cgccgagctg
     1501 gccgcggtga tccaccacca ggagttcacc cccacgctcg ccgccgtcgt ggaccaggtc
     1561 cccggcgtgc acaccttcct ctacgtggcc gacggctccg gcgtggagat ggccggcctg
     1621 cccaccgagg actacgcgac cgccttggcc gcggcctcca ccgagcggga cttcggcgag
     1681 cgttcgggcg acgacctcta catcctctac acgggtggca ccacgggcat gcccaagggc
     1741 gtgatgtggc ggcaggagga catcttcttc gcggccatgg acctctcggc catgggcctg
     1801 gccgaacctc cggccgcgcc cgaggacctg gccgcccggg tggaggagtc ggcggggacc
     1861 ctgaccatgg ccacagccgc ccccctcatg cacggcgctg cccagtgggc cgccttcatc
     1921 tgcttccacc aaggcaacac cctggtgctg gccgcctccc gcaagttcga tcccgagggc
     1981 atctggcgca tggtcgagca ggagaaggtc acgaccatga ccgtcgtagg cgacgcgatg
     2041 gcccgccccc tggccgacga gctgttggcc aatccggaca agtacgacct gtccagcttc
     2101 ttcgccctcg gctcgggcgg ggccatcctg tccgccggcg tgaaggagca gttcacccgg
     2161 gcccgacccg acctgatcat caccgacgcc tttggggcgt cggagaccgg cgccaatggt
     2221 ggcggggtcg gttcctccga cgaaggccac ccgcgtttcg ccgtgaacga ctcgaccgca
     2281 gtgctcgacg aggaccatcg ccccctcgag cccggctccg gcgtcaaggg catgctggcc
     2341 cgcaccggcc gcatcccgct cggctactac aaggacgagg agaagacggc ccgcaccttc
     2401 gtccacgacg ccgacggcaa gcgctgggtc atccccggcg acttcgccac cgtcgaggcc
     2461 gacggcacca tcaccctgtt cgggcgaggc tcggtcagca tcaactcggg cggtgagaag
     2521 atcttccccg aggaggtcga ggccgccctc aagagccacc ccgccgtctt cgacgccatc
     2581 gtcgtcggcg tcccggacga gcgcttcggc cagcgggtcg ccgccgtcgt cgccccgcgc
     2641 ccgggcacga gcccgagcc
//
LOCUS       DASTFV010000675         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2561561, whole genome shotgun sequence.
ACCESSION   DASTFV010000675 DASTFV010000000
VERSION     DASTFV010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2561561"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VFP02_12405"
     CDS             <1..679
                     /locus_tag="VFP02_12405"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET9543884.1"
                     /translation="RLVLVVACLLGAAVSCSSDGPTRVLLVGDSVMRQLGPALADQLP
                     DDVRVRNEAVSGSGLLSPWFVDWPRNLDRVIDRYDADVVVFLFVGNYDLGGPREFETA
                     SGEPIPDRTSPLFFRAWRAKAQQMTERAAEEADVTWILPPPMSGPDGQAVVDGLRAVY
                     RQVAEDTGAELIDAYDVLARPDGAFLGEAPGPEGAPVPLRSPDGVHLARVGAARLATL
                     VEDHLDL"
     gene            complement(719..1939)
                     /locus_tag="VFP02_12410"
     CDS             complement(719..1939)
                     /locus_tag="VFP02_12410"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9543885.1"
                     /translation="MTWAPPARPVWVERLIAHGDAVGGPEHLVGLDPDELVATAVAST
                     GRDDFGAPTWRPHYDLLLDALERESDLHLVGRLMARTEILRSLRNRLELTALWVEQPA
                     ILDQPVDGPVVVIGMARSGTSILHELLACDPAHRAPITWELLHPAHGPGEAEAWGDAT
                     HAFWHDVQPEYEAMHHNAGDLPNECIFATMNEFLSDHWGGVHEVPSYSMYLAGADHTD
                     AYRYHRCILQTLQAREPGERWLLKAPSHLATLDALFAVYPDARIVHVHRDPLKTVPST
                     LSLMATLKWMRGRHTDPVATAPLLAWGFGALLDGVIDGRASGRLPDAQFADIRYADLL
                     ADPVAAVARVYEAFGWDYVPSTRQAMLDHLATRPQDKHGTHRYALEDFGLDPTDERAR
                     FTPYQQRYQVPDEA"
     gene            complement(2007..>2299)
                     /locus_tag="VFP02_12415"
     CDS             complement(2007..>2299)
                     /locus_tag="VFP02_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543886.1"
                     /translation="TSTNGVQAEVDADGRVRLVLAHADPGTANWIDTQGRPEGLLVYR
                     YVGTRTRPVPEALVVPLADVGDHLPADHPRVSPDARRTQLAARRRAVLARYQ"
BASE COUNT          289 a          785 c          889 g          336 t
ORIGIN      
        1 gcggctggtc ctcgtggtcg cctgcctgct cggggcggcg gtgtcctgct cgtcggacgg
       61 accgacccgg gtgctgctgg tcggtgactc ggttatgcgc cagctcggcc cggcgctcgc
      121 cgaccagctc cccgatgacg tgcgcgtccg caacgaggcc gtctcgggct cgggcctgct
      181 gagcccgtgg ttcgtcgact ggccgaggaa cctggatcgc gttatcgacc ggtacgacgc
      241 cgacgtcgtc gtcttcctct tcgtgggcaa ctacgacctc ggtggtcccc gcgagttcga
      301 gacggcatcg ggcgagccga tccccgatcg gacctccccg ctcttcttcc gggcctggag
      361 ggcgaaggcg cagcagatga ccgaacgggc ggccgaggag gccgacgtca cctggatcct
      421 gccgccgccg atgtccggcc ctgacggcca ggccgtggtc gacggcctcc gggccgtcta
      481 ccggcaggtg gccgaggaca ccggggccga gctcatcgac gcctacgacg tcctcgctcg
      541 gccggacggc gccttcctgg gcgaggcccc ggggccggag ggtgccccgg tcccccttcg
      601 ctcccccgac ggcgtccacc tcgcccgggt cggcgccgcc cgcctcgcca ccctcgtcga
      661 ggaccacctc gacctctgac ctgtccacca ctcaccgcag gttccacgac ggaggtgatc
      721 aggcctcgtc ggggacctgg tagcgctgtt ggtagggcgt gaagcgggcg cgttcgtcgg
      781 tggggtcgag accgaagtcc tcgagggcgt agcggtgggt gccgtgcttg tcctggggcc
      841 gggtggcgag gtggtcgagc atcgcctggc gggtcgacgg gacgtagtcc cagccgaagg
      901 cctcgtagac gcgggcgacg gcggcgaccg gatccgccag caggtcggcg tagcggatgt
      961 cggcgaactg ggcgtcgggc aggcgaccgg aggcccggcc gtcgatcacc ccgtccagca
     1021 gtgccccgaa gccccaggcc agcagcgggg ccgtggcgac agggtcggtg tggcgaccgc
     1081 gcatccactt cagtgtcgcc atcaggctca gggtggaggg caccgtcttc agcgggtccc
     1141 ggtggacgtg gacgatgcgg gcgtcggggt agacggcgaa cagggcgtcg agcgtggcca
     1201 ggtggctggg cgccttgagc agccatcgct caccgggttc ccgggcctgg agggtctgca
     1261 ggatgcaccg gtggtagcgg taggcgtcgg tgtggtcggc gccggccagg tacatcgagt
     1321 agctgggcac ctcgtggacc ccaccccagt ggtccgagag gaactcgttc atggtggcga
     1381 agatgcactc gttcgggagg tccccggcgt tgtggtgcat ggcctcgtac tcgggctgga
     1441 cgtcgtgcca gaaggcgtgg gtggcgtcgc cccacgcctc ggcctccccc gggccgtggg
     1501 ccgggtgcag cagttcccag gtgatcggcg cccgatgggc cgggtcgcag gccaagagct
     1561 cgtgcaggat cgacgtcccc gagcgggcca tgccgatgac gaccaccggg ccgtcgaccg
     1621 gctggtcgag gatcgcgggc tgctcgaccc acagcgcggt cagttcgagg cggttgcgca
     1681 gcgaccgcag gatctccgtc cgggccatca ggcgcccgac caggtgcagg tcgctctccc
     1741 gctccagcgc gtcgaggagc aggtcgtagt gcggccgcca ggtcggcgcc ccgaagtcgt
     1801 cccgcccggt cgacgcgacg gcggtggcga ccagctcgtc agggtcgagt ccgaccaggt
     1861 gctcaggccc gcccaccgcg tcgccgtggg cgatcaggcg ctcgacccag accggccggg
     1921 ccggcggcgc ccaggtcacg gggtgccacc cacgaacggg cgccgggtac accacggatc
     1981 cgtggtgtgg tcaacgccag ttcgggtcac tggtagcgag ccaggacggc gcggcggcgg
     2041 gcggccagct gggtgcggcg cgcgtccggg ctgacgcggg ggtggtcggc gggcaggtgg
     2101 tcaccgacgt cggccagggg cacaaccagg gcctcgggca ccgggcgggt ccgggtgccg
     2161 acatagcggt agacgagcag gccttcgggc cggccctggg tgtcgatcca gttggccgta
     2221 cccgggtcgg cgtgggccag gacgaggcgg actcggccgt cggcgtccac ctcggcctgg
     2281 actccgttcg tgctggtca
//
LOCUS       DASTFV010000676         2690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2580603, whole genome shotgun sequence.
ACCESSION   DASTFV010000676 DASTFV010000000
VERSION     DASTFV010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2580603"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VFP02_12420"
     CDS             complement(<1..829)
                     /locus_tag="VFP02_12420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET9543887.1"
                     /translation="MDLRIFTEPQFGATYGDQLAVAQAAEAGGFDAFFRSDHWQTFGG
                     DGLPGPTDSWVTLGAIARETERIRLGTLVTSATFRYPGPLAVAVAQVDEMSGGRVELG
                     IGAGWFDGEHDAHGIPFPATGERFDRLEEQLAVITGLWETPVGETFSFDGQHYPVKNS
                     PALPKPVQSPRPPVIIGGHGPRRTPRLAATHADEFNLGFSPIADFTAQMERVTAACEA
                     RDRDPATMTWSVALTAVMGADEAEFQRRAEAIGQDPEGLRSGQLGGTPDQIRDRLGQW
                     "
     gene            914..1978
                     /locus_tag="VFP02_12425"
     CDS             914..1978
                     /locus_tag="VFP02_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9543888.1"
                     /translation="MAHIDFDRVTKRFGDVEAVSDLSLHVDDGDFLVLLGPSGCGKST
                     LLRMLAGLDEPTEGVIEVGGEPVNNVEPKDRDIAMVFQSYALYPHKTVRANIEFPLRP
                     RKVPKDERQTMVEEAAAQLGLTALLDRKPGQLSGGQRQRVALARAIVRRPVAFLMDEP
                     LSNLDAKLRTQTRAELVELQRRLGTTFVYVTHDQVEAMTMAKRVAVMSEGVLQQVGPP
                     TEVYDKPANLFVARFIGNPPMNTLPGTVDGATITVAGGTVPSPTTSAALPTEVVVGIR
                     PERLSVRRPDDAGIQVKLRVLEALGHEQLLVCDAEDGSTVTVRLDPDLSAPAEGSTVN
                     LVPDPGHVHLFDPTTTERID"
     gene            1991..>2690
                     /locus_tag="VFP02_12430"
     CDS             1991..>2690
                     /locus_tag="VFP02_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9543889.1"
                     /translation="MTMQGVPTFGDGDDGGLGRKKLSEIPLALLLLAPSLIIFGVFVF
                     YPMGRTIWLGFYREDPFGGRVYAGFSQYADVLSSAEFRHSLWVTILFATITVGFGLVF
                     GLTLATLAHQRMKGITVYRTIFSSTVATSVAVASLIWLVLFQPSIGIVNQFLKSIGQE
                     PVQWLLDSSTALFAVAATTVWQNLGITFIILIAGLQGIPDDLYESARVDGASSWLQFR
                     EITVPMLSPTLLFAS"
BASE COUNT          387 a          959 c          922 g          422 t
ORIGIN      
        1 cccactggcc gaggcggtcc cggatctggt cgggcgtgcc gccgagctga cccgagcgca
       61 gcccttcggg gtcctggccg atggcctcag cccgtcgctg gaactcggcc tcgtcggccc
      121 ccatcaccgc ggtcagggcc acggaccagg tcatcgtggc ggggtcccgg tcccgggcct
      181 cgcaggccgc ggtcacccgc tccatctggg cggtgaagtc ggcgatcggg gagaagccga
      241 ggttgaactc gtcggcatgg gtggcggcca gccgcggtgt ccgcctcgga ccgtggccgc
      301 cgatgatgac cggcggccgc ggcgactgca cgggcttggg cagagcgggg gagttcttga
      361 cggggtagtg ctgtccgtcg aacgagaagg tctcgccgac cggtgtctcc cacaggccgg
      421 tgatgaccgc cagctgctcc tcgagccggt cgaagcgctc gcccgtggca gggaacggga
      481 tgccgtgggc gtcgtgctcg ccgtcgaacc agccggcgcc gatgcccagc tcgacccggc
      541 ccccgctcat ctcgtcgacc tgggccaccg cgacggccag cggtcccggg tagcggaagg
      601 tggccgaggt gaccagcgtg cccagccgga tgcgctcggt ctcgcgggcg atggcgccga
      661 gggtcaccca ggagtcggtc gggcccggca gcccgtcgcc gccgaaggtc tgccagtggt
      721 cggagcggaa gaaggcgtcg aagccgcccg cctcggccgc ctgagcgacc gcgagctgat
      781 cgccgtaggt ggcgccgaac tggggctcgg tgaagatccg gaggtccatc cggtctgtct
      841 acccgaggtc acccggccgt tcgaccgccc gtcacccgct cctggctagg gtccgcgccg
      901 gagagagagg accatggcgc acatcgactt cgatcgggtc accaagcgct tcggcgacgt
      961 cgaagcggtg tccgatctca gcctgcacgt ggacgacggc gacttcctgg tcctgctcgg
     1021 gccgtcgggc tgtggcaagt ccaccctgct gcggatgctg gccggtctgg acgagccgac
     1081 cgagggcgtc atcgaggtcg gcggtgagcc ggtcaacaac gtcgagccga aggaccggga
     1141 catcgcgatg gtgttccaga gctacgccct ctacccccac aagacggtgc gggccaacat
     1201 cgagttcccg ttgcggcccc gcaaggtccc caaggacgag cgccagacga tggtcgagga
     1261 ggccgctgcc cagctcgggc tgacggcact actggaccgc aagccgggcc agctctccgg
     1321 cggtcagcgc cagcgggtgg ctctggcccg ggccatcgtg cgccgtccgg tcgccttcct
     1381 catggacgag ccgctgtcga acctcgacgc caagttgcgg acccagaccc gggccgagct
     1441 ggtggagctc cagcgccggc tcggcaccac cttcgtctac gtgacccacg accaggtcga
     1501 ggccatgacg atggccaagc gagtggcggt catgagcgag ggcgtgctcc agcaggtcgg
     1561 cccgcccacc gaggtctacg acaagccggc caacctcttc gtggcccgct tcatcggcaa
     1621 cccgccgatg aacaccctgc ccggcaccgt cgacggggcg acgatcaccg tggccggtgg
     1681 gacggtgccg tcaccgacca cctcggctgc cctgccgacc gaggtcgtgg tcgggatccg
     1741 gccggagcgc ctgtcggtcc gccgtcccga cgatgccggc atccaggtga agctccgggt
     1801 gctggaggcc ctcggtcacg aacagctgct cgtctgcgac gccgaggacg gctcgacggt
     1861 gacggtccgt ctcgatccgg acctctccgc cccggccgag ggctccacgg tcaacctcgt
     1921 gcctgacccc ggtcacgtcc acctcttcga cccgaccacg accgagcgga tcgactgagg
     1981 tgcccaaccg atgaccatgc aaggggtgcc gacgttcggt gacggcgatg acggtggcct
     2041 cggtcgcaag aagttgagcg agatcccgct ggcccttctc ctgctcgccc cgtcactgat
     2101 catcttcggg gtcttcgtct tctacccgat gggtcgcacc atctggctcg gcttctaccg
     2161 ggaggacccc ttcggcgggc gggtctacgc cgggttcagc cagtacgccg acgtgctcag
     2221 ctcggcggag ttccggcaca gcctgtgggt gacgatcctg ttcgccacga tcaccgtggg
     2281 cttcgggctg gtcttcggcc tgacgttggc cacgctcgcc caccagcgga tgaaggggat
     2341 cacggtctac cgcacgatct tctcgtcgac cgtggccacc tcggtggcgg tggcgtcgct
     2401 catctggctg gtcctgttcc agccgtcgat cgggatcgtc aaccagttcc tgaagtcgat
     2461 cggccaggaa cccgtccagt ggctgctcga cagcagcacc gcgctgttcg cggtggccgc
     2521 caccaccgtc tggcagaacc tcggcatcac cttcatcatc ctcatcgccg gcctccaggg
     2581 catccccgac gacctctacg agagcgctcg ggtcgacggg gccagcagct ggctccagtt
     2641 ccgggagatc acggtcccca tgctctcgcc cacgctgctg ttcgccagcg
//
LOCUS       DASTFV010000677         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2581028, whole genome shotgun sequence.
ACCESSION   DASTFV010000677 DASTFV010000000
VERSION     DASTFV010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2581028"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VFP02_12435"
     CDS             <1..717
                     /locus_tag="VFP02_12435"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9543890.1"
                     /translation="ENPKRVYGERKQFPSTRLGTAAAIRDALVKTANYLAKRDHDGDE
                     PAERDLRWEALGLVLTGEIPWRQHCHRADDIATAMRLADEFGYRLVIDHGTEAVLLAD
                     RLAERGIPVLIGPLLTSRSKVELRNRSLANPARLAAAGVELGIITDHPVVPVHLLHVQ
                     AALAVREGLDPADALRAVTLTPARILGLEDRLGSLEPGKHATLCVWSGDPLDARSRVE
                     AAWIEGRQVYQAGPDHPSSF"
     gene            complement(722..1687)
                     /locus_tag="VFP02_12440"
     CDS             complement(722..1687)
                     /locus_tag="VFP02_12440"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET9543891.1"
                     /translation="MQAIVQDRFGRPDVLELREVDQPAVGDDEVLVRVRAASVNPADW
                     YAMAGFPYVARPQMGLRAPRARLGLDVAGEVAAVGERVTRFKAGDEVFGAGTGTLAEY
                     AAVPEDALVAKPANLSFEQAAAVPVAGLTALQGLRDKGGIQPGRRVLINGASGGVGTF
                     AVQVAKAFGADVTGVCSPRNVDLVASLGADQVIDYTRQDFTRNGRRYDLLLDVAGSRP
                     WSACRRVLDPGATLVLVGAPKGSRLLGPLGHILGVRLASLRASQTVRFFISTARTADL
                     AALGELLAAGTVTPVVERSYALRDAAEALRYLGQGHAKGKLVVTV"
     gene            complement(1715..>2281)
                     /locus_tag="VFP02_12445"
     CDS             complement(1715..>2281)
                     /locus_tag="VFP02_12445"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET9543892.1"
                     /translation="VPGLKIAAPSTPADMVGLMAAAIRSDDPVLVFEHKSLFATKGDP
                     PPSGDHVLPLGAAEVRRPGGDVTLVALASTVPTAMAAAEELATDGVDAEVIDLRCLIP
                     LDARTVLGSVARTSRLVIVEENPYQGGWGGTVASIVAEEGFYDLDAPIRRVAGACVPL
                     PFAANLEQAVTPTADRVATAVREALDAG"
BASE COUNT          303 a          890 c          813 g          275 t
ORIGIN      
        1 gagaacccga agcgggtcta cggcgagcgc aagcagttcc cctcgacccg gctggggacg
       61 gccgcggcga tccgcgacgc cctcgtcaag acagccaact acctggccaa acgggaccac
      121 gacggcgacg agccggccga acgggacctg cgctgggagg cgctcggcct ggtcctgacc
      181 ggcgagatcc cctggcgcca gcactgccac cgggccgacg acatcgccac cgccatgcgc
      241 ctggccgacg agttcggcta ccgcctggtg atcgaccacg gcaccgaggc cgtcctgctc
      301 gccgaccgcc tggccgagcg cggcatcccc gtcctgatcg gccccctgct gacctcccgc
      361 tccaaggtcg agctgcgcaa ccgctccctg gccaacccgg cccggctggc cgccgccggg
      421 gtcgagctcg gcatcatcac cgaccacccg gtcgtccccg tccacctgct ccacgtccag
      481 gccgccctgg ccgtccgcga aggcctcgac ccggccgacg ccctgcgcgc cgtcaccctc
      541 accccggccc gcatcctcgg cctggaggac cgcctcggct ccctcgagcc cggcaagcac
      601 gccaccctgt gtgtctggtc cggcgacccc ctcgacgccc gctcccgcgt cgaggccgcc
      661 tggatcgagg gccgccaggt ctaccaggcc ggccccgacc acccgtcgtc gttctgaacg
      721 gtcacacggt gacgacgagc ttgcccttgg cgtgtccctg gcccaggtag cgcagcgcct
      781 ccgcggcgtc gcgcagcgcg tagctccgct ccaccaccgg ggtgaccgtc ccggccgcga
      841 gcagctcccc cagggccgcc aggtctgcgg tgcgcgccgt cgagatgaag aagcgcaccg
      901 tctggctcgc ccgcagcgag gccaggcgga ccccgaggat gtgacccagc ggcccgagca
      961 gccggctgcc cttgggcgcc ccgaccagga cgagggtcgc ccccgggtcg agcacccgcc
     1021 ggcacgccga ccacggcctg ctcccggcga cgtcgagcag caggtcgtag cggcggccgt
     1081 tcctggtgaa gtcctgccgg gtgtagtcga tgacctggtc ggcgccgagc gaggcgacca
     1141 ggtccacgtt cctggggctg cacacgccgg tcacgtcggc gccgaacgcc ttggcgacct
     1201 gcaccgcgaa ggtgccgaca ccgcccgacg ccccgttgat caggacccgc cgccccggct
     1261 ggatgccgcc cttgtcacgg agcccctgga gggcggtgag gccggccacg gggacggcgg
     1321 cggcctgctc gaacgacagg ttggccggct tggccaccag cgcatcctcg ggaaccgccg
     1381 catactcggc cagggtcccg gtgcccgcgc cgaacacctc gtcgcccgcc ttgaagcgcg
     1441 tcacccgctc gccgacagcc gccacctcgc ccgcgacgtc caggcccagc ctggccctcg
     1501 gcgcccgcag ccccatctgg gggcgggcga cgtaggggaa tccggccatg gcgtaccagt
     1561 ccgccgggtt caccgacgcc gcgcggaccc gcaccagcac ctcgtcgtcg ccgacagctg
     1621 gctggtcgac ctccctgagc tcgagcacgt ccggtctgcc gaaccggtcc tggacgatcg
     1681 cctgcatcag gcgctccccg gcggcggcac cgtgctagcc ggcatcgagc gcctcgcgaa
     1741 ccgcggtggc gacccggtcg gcggtggggg tgacggcctg ctccaggttg gcggcgaagg
     1801 ggagggggac gcaggcgccg gcgacccggc ggatgggggc gtcgaggtcg tagaagccct
     1861 cctcggcgac gatggaggcg acggtgccgc cccagccgcc ctggtagggg ttctcctcga
     1921 cgatcaccag gcggctggtg cgggcgaccg agcccagcac cgtgcgggcg tccaggggga
     1981 tcaggcagcg caggtcgatc acctcggcgt cgaccccgtc ggtggccagc tcctcggccg
     2041 cggccatggc ggtggggacg gtcgaggcca gggcgaccag ggtgacgtcg cctccggggc
     2101 ggcggacctc ggcggcgccg agggggagca cgtggtcgcc ggagggtggc gggtcgccct
     2161 tggtggcgaa cagcgacttg tgctcgaaca ccagcaccgg gtcgtcgctg cggatggcgg
     2221 cggccatcag cccgaccatg tcggccgggg tcgagggggc ggcgatcttg aggccgggga
     2281 c
//
LOCUS       DASTFV010000678         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2585649, whole genome shotgun sequence.
ACCESSION   DASTFV010000678 DASTFV010000000
VERSION     DASTFV010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2585649"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..158
                     /locus_tag="VFP02_12450"
     CDS             36..158
                     /locus_tag="VFP02_12450"
                     /inference="COORDINATES: protein motif:HMM:NF023816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543893.1"
                     /translation="MRISEKIVVLDYGAKIAEGDPDVVRNDPRVIEAYLGGGDS"
     gene            164..865
                     /locus_tag="VFP02_12455"
     CDS             164..865
                     /locus_tag="VFP02_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9543894.1"
                     /translation="MLEVTDLEAGYGPVQVLRGMSFTVDPGETVVILGANGAGKTTTI
                     RAISGMIVTKGSVIVDGRSVGGKKPEEIVRRGVAHVPQGRGTFVDLTVEENLRLGAFT
                     RKDADIAADIDHWYEVFPRLQERRDQVAGGMSGGEQQMLAIARAAMSRPRLLLLDEPS
                     LGLAPLVTQELFRRLQVLNQEQGTALLVVEQNANLALGIAERGYVLEAGLIVAQGTAA
                     ELQADESVRKAYLGI"
     gene            878..1759
                     /locus_tag="VFP02_12460"
     CDS             878..1759
                     /locus_tag="VFP02_12460"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9543895.1"
                     /translation="MELFFNQVINGIQQGAIYAALALALVLIFRSTSLLNFAQGEMAM
                     FSTFIAWILADNGFPVVFAILAAMAVSVVGGAIIERTLIRPVGSDNVLAIVIVTIGIF
                     IATNALAGWIYGTDGRVFPRIFPNESLVTFGDVTVTKETTGIVAILLVVVGLLYLLFQ
                     HTKVGLAMRAVASNAESAQLVGIRVGRILMLGWGLAAALGALAGALAAPQLNLSLNLM
                     QGLLIYAFAAATLGGFDSPLGAVVGGLIVGVTQTLAGQYIDFFNGFELGSAFLLILIV
                     LLVRPQGLFGKAVVTRV"
     gene            1756..2835
                     /locus_tag="VFP02_12465"
     CDS             1756..2835
                     /locus_tag="VFP02_12465"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9543896.1"
                     /translation="MSRLALTPRRVVLGIVAVALAALVLYIPQYFVEFRTAQLTSVIA
                     ISIAVLGLGLLTGFNGQISVGHGAFFGVGAYTTAILTADHGWSHLPTIPVAMVLCLAI
                     GVLVGLPALRISGLYLALVTLALATLFPLVIQKYSDVTGGSTGIRVPSFEPPDWASGL
                     TEDQWAYYVVLAFAVVTFVFVRNLIRSRVGRAIIAIRDGEVAAEVLGVSLARYKVATF
                     GISAMIAGLGGSLFVMNTAVLDRVDPSQYTISRSIEFLAALVIGGAATILGPVLGSLF
                     IVFVPELSSDVNAELSRVIFGGVLIVMMLVLPGGIVGGLRQLEATVLRRSGRDGQWRK
                     VRRGPDPAAATPDPGGSPTVDDAAV"
     gene            2856..>3170
                     /locus_tag="VFP02_12470"
     CDS             2856..>3170
                     /locus_tag="VFP02_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543897.1"
                     /translation="MIRSNRLGALGGLLVVLALIAAACGGGREDTGGDSDANEAAAEG
                     TSDEFAEYLHLDDCTTDFTQGVTDDSIKIGSSFPQSGTFAAFAEISTGYQAYFDYVNE
                     ELG"
BASE COUNT          434 a         1131 c         1061 g          544 t
ORIGIN      
        1 ccgtcctgct cgtcgagcac cacatggaga tggtcatgcg catctccgag aagatcgtgg
       61 tcctcgacta cggcgccaag atcgccgagg gcgatccgga cgtcgtccgc aacgatcccc
      121 gggtcatcga ggcctatctg ggcgggggcg actcgtgacc ccgctgctgg aggtcaccga
      181 cctcgaggcc ggctacggcc ccgttcaggt cctgcggggg atgtctttca ccgtcgaccc
      241 gggcgagacg gtggtcatcc tcggggccaa cggggcgggc aagacgacga ccatccgcgc
      301 catcagcggg atgatcgtca ccaaggggtc ggtcatcgtc gacggcaggt cggtgggcgg
      361 caagaagccg gaggagatcg tccgacgggg cgtggcccac gtcccccagg ggcggggcac
      421 cttcgtggac ctcacggtgg aggagaacct ccgcctcggc gccttcaccc gcaaggacgc
      481 cgacatcgcc gccgacatcg accactggta cgaggtcttc ccccgcctgc aggagcgccg
      541 cgaccaggtc gccggcggga tgagcggggg cgagcagcag atgctggcga tcgcccgggc
      601 ggccatgtcc cggccccgcc tcctgctgtt ggacgagccg tcgctgggcc tggccccgct
      661 cgtgacccag gagctgttcc gacggctgca ggtgctgaac caggaacagg ggaccgccct
      721 gctcgtcgtg gagcagaacg ccaacctggc cctcggcatc gccgagcggg gctatgtgct
      781 cgaggccggc ctgatcgtgg cccagggcac ggcggcggag ctgcaggccg acgagtccgt
      841 gcgcaaggcg tacctcggga tctgagggga ggcaaccgtg gaactgttct tcaaccaggt
      901 gatcaacggc atccagcagg gcgccatcta cgccgcgctg gcgctggccc tcgtcctgat
      961 cttccgctcc acctcgttgc tgaacttcgc ccagggcgag atggccatgt tctcgacctt
     1021 catcgcctgg atcctggccg acaacgggtt ccccgtcgtg ttcgccatcc tcgccgccat
     1081 ggccgtctcg gtggtcggcg gggcgatcat cgaacggacc ctgatccggc ccgtcggcag
     1141 cgacaacgtc ctggccatcg tcatcgtgac catcggcatc ttcatcgcca ccaacgcgct
     1201 ggcggggtgg atctacggca ccgacggccg ggtgttcccc cgcatcttcc ccaacgagtc
     1261 gctggtcacc ttcggggacg tcaccgtgac caaggagacg acggggatcg tcgcgatcct
     1321 gctcgtggtc gtcgggctgc tctacctgct gttccagcac accaaggtcg ggctggccat
     1381 gcgggccgtg gcctcgaacg cggagtccgc ccagctggtc ggcatccggg tgggccggat
     1441 cctcatgctg gggtggggcc tggccgcggc cctcggcgcc ttggccggcg ccctggccgc
     1501 accgcagctg aacctgagcc tgaacctgat gcagggcctg ctcatctatg ccttcgcggc
     1561 ggcgaccctc ggtgggttcg acagcccgct gggcgcggtg gtcggcggcc tcatcgtcgg
     1621 cgtgacccag accctggccg gccagtacat cgacttcttc aacggcttcg agctgggctc
     1681 ggccttcctg ctcatcctca tcgtgctgct ggtgcgcccc cagggcctct tcggcaaagc
     1741 ggtggtcacc cgcgtatgag tcgtctcgcg ctcacgcccc gtcgggtggt cctgggcatc
     1801 gtcgccgtgg ccctcgccgc gctcgtcctc tacataccgc agtacttcgt cgagttccgg
     1861 acggcgcagc tcacgagcgt catcgccatc tcgatcgccg tgctcggcct cgggttgctg
     1921 accgggttca acggccagat ctcggtcgga cacggcgcgt tcttcggcgt cggggcctac
     1981 acgaccgcca tcctcaccgc cgaccacggg tggtcgcacc tgccgaccat tccggtggcg
     2041 atggtgctct gcctggccat cggcgtgctc gtcggcctgc ccgccctgcg catcagcggc
     2101 ctgtacctcg ccctggtcac cctcgccctg gccacgctct tcccgctggt catccagaag
     2161 tactccgacg tcaccggcgg gagcaccggc atccgggtgc cgtcgttcga gcccccggac
     2221 tgggcgtcgg gcctgaccga ggaccagtgg gcctactacg tggtcctggc cttcgccgtg
     2281 gtcaccttcg tcttcgtgcg caacctcatc cgcagccggg tgggccgggc catcatcgcc
     2341 atccgtgacg gcgaggtcgc ggccgaggtg ctgggtgtca gcctggcccg ctacaaggtg
     2401 gccaccttcg ggatcagcgc catgatcgcc ggtctgggcg gatcgctctt cgtgatgaac
     2461 accgccgtgc tggaccgcgt cgacccttcc cagtacacga tcagccgctc catcgagttc
     2521 ctggcggccc tggtcatcgg cggcgccgcc accatcctcg gcccggtcct cggctcgctg
     2581 ttcatcgtct tcgtccccga gctctcctcc gacgtcaacg ccgagctgtc ccgggtcatc
     2641 ttcggggggg tgctcatcgt catgatgctg gtgttgcccg gcgggatcgt cggtggcctc
     2701 cggcagctgg aggccacggt gctgcgccgg tcgggccggg acgggcagtg gcgaaaggtc
     2761 cgtcgggggc cagatccggc cgccgccacc cccgatccgg gtgggtcgcc tacggtggat
     2821 gacgctgcag tctgagcacc gaggggagca atcgaatgat ccgatccaac cgtttgggcg
     2881 cgctcggcgg gttgctcgtg gtcctcgccc tgatcgccgc cgcctgcggt ggcggtcgtg
     2941 aggacaccgg gggcgacagc gacgccaacg aagccgccgc ggaggggacc agcgacgagt
     3001 tcgccgagta cctgcacctc gacgactgca ccaccgactt cacccagggc gtcaccgacg
     3061 actccatcaa gatcgggtcc agcttcccgc agtcgggcac cttcgccgcc ttcgccgaga
     3121 tctcgaccgg ctaccaggcc tacttcgact acgtgaacga ggagctgggc
//
LOCUS       DASTFV010000679         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2596369, whole genome shotgun sequence.
ACCESSION   DASTFV010000679 DASTFV010000000
VERSION     DASTFV010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2596369"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1294
                     /locus_tag="VFP02_12475"
     CDS             158..1294
                     /locus_tag="VFP02_12475"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9543898.1"
                     /translation="MKNPMRAFLLSLVAILGLLPVTAVQADPLPPPPGHGDIEVVGGQ
                     DADPGEDPWMAALVVDPRLTPNPFAGLICGASFISPDTVVTAAHCVLDFPVQFLDIVA
                     GSNNLIPAELERLDIRNVRIHPLNDPSTALYDVAAIQLVHPPSFPVTPIDLVTPADAA
                     LWEPGDLARFTGWGADETGQPISRLQEAESPIVADAECRARYTSAFFRGRTMVCAGDI
                     SAGADLVSPCFGDSGGPLTVLDGSRPVLIGLVLGGLRCGDPNYPAIFSQVSAFTDFLE
                     PYLDPDDVPLRVQELRAGRRGPEGRAAVVSWRAPLFDGGAPINHYIVTVQPGDRRYVT
                     GGRARTLRIPHISPSQPIVIGVSARNRIGEGPVASVRLRRAPIN"
     gene            1313..>2164
                     /locus_tag="VFP02_12480"
     CDS             1313..>2164
                     /locus_tag="VFP02_12480"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET9543899.1"
                     /translation="MKTVRRLLPAVLAALALLPLAAASAQEAPPETTPDGEIIGGTRA
                     DPHEYPFQVALLQHGVGDRWASQFCGGTLISPDTVLTAGHCVVGTRAGAIDILAGTNR
                     LLPGGGQRVPARAVRLHPDYDDTTLQNDIAIIQLGVELPYETVAPAVAGQEALYPPGT
                     MATTIGWGDRDIRLDLQYYPLYLREVEVPIVSDDDCSAAYPGELVADQMICAGDMVDG
                     GEDSCYGDSGGPLMVPNGEHWLQVGVVSTGRGCARRPFPGIYTEVAAYEDFVTRYLDP
                     DTVPDPIT"
BASE COUNT          296 a          872 c          680 g          316 t
ORIGIN      
        1 ggcaaccacc cgcaggccta cagccacctc ggcctgatcc acaacgccct caaccttgct
       61 gcgtacgccc gcgacggccg gccgccggcc gaccgggagg cgtgagtgtc ctggatgtag
      121 ggtcctgcca ggcggacaat cggaatcgga gcacctcgtg aagaacccca tgcgcgcgtt
      181 cctgctgtcc ctcgtcgcga tcctcgggct gctcccggtc acggccgtgc aggcggaccc
      241 gctgccgccg ccgccggggc acggcgacat cgaggtggtc ggcggccagg acgccgaccc
      301 cggtgaggac ccgtggatgg ccgccctcgt cgtcgacccg cgcttgaccc ccaacccgtt
      361 cgccggcctg atctgcggtg cgtcgttcat ctccccggac acggtggtca ccgcggccca
      421 ctgcgtcctc gacttccccg tccagttcct cgacatcgtc gcgggaagca acaacctgat
      481 ccccgctgag ctcgagcgcc tcgacatccg caacgtccgg atccatccgc tgaacgaccc
      541 ctccaccgcg ctgtacgacg tcgccgccat ccaactcgtc catccgccgt ccttccccgt
      601 gacccccatc gacctggtca cgccggctga cgccgccctc tgggagccgg gcgacctggc
      661 tcgcttcacc ggctggggcg cggacgagac cggtcagccg atcagccgcc tgcaggaggc
      721 cgagtcgccg atcgtggccg acgccgagtg ccgggcccgc tacaccagcg ccttcttccg
      781 cgggcgcacg atggtctgcg ccggcgacat cagcgccggg gccgacctcg tcagcccctg
      841 cttcggcgac agcggtggtc ccctgaccgt gctcgacggc agccgccccg tcctcatcgg
      901 cctcgtcttg ggcgggctgc gctgtggtga cccgaactac cccgcgatct tcagtcaggt
      961 cagcgcgttc accgacttcc tcgagcccta cctggatccc gacgacgtcc ccctgcgcgt
     1021 gcaggagctt cgggcgggtc ggcgcggtcc cgagggccgg gccgccgtgg tgagctggcg
     1081 ggcgcctctg ttcgacggtg gcgccccgat caaccactac atcgtgaccg tccagcccgg
     1141 ggaccgccgg tacgtgaccg gcggtcgggc ccgtaccctc cgcatcccgc acatctcgcc
     1201 cagccaaccg atcgtgatcg gcgtctccgc ccggaacagg atcggcgaag ggcccgtcgc
     1261 ctccgtcagg ctgcgccgcg ccccgatcaa ctgacctctc tggagacctc gtttgaagac
     1321 cgtccgtcgc ctgctccccg ccgtcctggc cgccctcgcc ctgcttccgc tcgccgcggc
     1381 gtcggcccag gaggcccccc cggagaccac ccctgacggc gagatcatcg gcgggacccg
     1441 ggccgacccg cacgagtacc cgttccaggt cgccctcctc caacacggcg taggcgaccg
     1501 ctgggcgtcc cagttctgcg gcggcaccct gatctcgccc gacaccgtcc tcacggccgg
     1561 gcactgcgtc gtgggcaccc gggccggcgc catcgacatc ctggccggca ccaaccgcct
     1621 cctgcccgga gggggtcaac gggtcccggc tcgggcggtc cgtctccacc ccgactacga
     1681 cgacacgacg ctccagaacg acatcgccat catccagctc ggcgtcgagc tgccctacga
     1741 gaccgtcgcc ccggccgtcg ccggccagga ggcgctctac ccaccgggga ccatggccac
     1801 caccatcggc tggggcgacc gggacatccg cctggacctc cagtactacc cgctctacct
     1861 gcgcgaggtc gaggtcccga tcgtgtccga cgacgactgc agcgcggcct accccggcga
     1921 gctggtcgcg gaccagatga tctgtgccgg cgacatggtc gacggtggtg aggactcctg
     1981 ctacggggac agcggcggcc cgctgatggt gcccaacggc gagcactggc tgcaggtcgg
     2041 cgtggtcagc accggccggg gctgcgcccg gcgacccttc cccggcatct acaccgaggt
     2101 cgcggcctac gaggacttcg tcacccgcta cctggacccc gatacggtgc ccgatccgat
     2161 cacc
//
LOCUS       DASTFV010000680         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2600031, whole genome shotgun sequence.
ACCESSION   DASTFV010000680 DASTFV010000000
VERSION     DASTFV010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2600031"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1750)
                     /locus_tag="VFP02_12485"
     CDS             complement(<1..1750)
                     /locus_tag="VFP02_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1478..1480),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9543900.1"
                     /translation="MPGRNRRDRRAPGARLERPLVREDGVLRPATWEEALDRATEGFR
                     RVLDTKGPTGIGLFSCSKATNEVNYAAQKFARTALGSNYIDSCNRTUHAPSVVGLATV
                     FGAGGGTCSYEEVEEADVVLLWGSNAREAHPIYFHHLLKGIENGARLYCVDPRRTSSA
                     KFADVWLGLEVGSDIALANALGREIIAAGLTDDAFIAHSTQGYDAYAAAVEPYTLEAA
                     AALTGLPADAIRDMAHAYARADKAQILWTLGITEHHNAVDNVVALCNLALLTGHVGRW
                     GSGLVPLRGQNNVQGGGDMGALPNKLPGFQDIEDPAARARFEAVWGVPLNPEPGLHLT
                     LMFEAMGRGELTGLYVIGENPADSEADVEHARSLLAGLDHLVVQDIFLTRTAELADVV
                     LPASVAWAECEGTVTSSERRVQRVRAAVPPPGEARHDHEILGELARRMGYAWGDPTPE
                     DLWDELRSLSPMHQGMTYARLEAEGGLQWPCPTEDHPGSPFLHGWLWEPDLGGRDPAP
                     FSVVEHAGPVEELSEEFPLRLTTGRALDSFNTGVQSGGFDSPIRYGEALDVNPVDAAR
                     LGVVDGERVLVTSPRGA"
     gene            complement(1762..>2193)
                     /locus_tag="VFP02_12490"
     CDS             complement(1762..>2193)
                     /locus_tag="VFP02_12490"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HET9543901.1"
                     /translation="DRLDPLPAVEPFPPEVLLEVTDKAAVDQDLFAATGAVHAAAAFG
                     RDGSPFLLREDIGRHNAVDKVIGRLLLDGALPAMDLGLFVSGRASFEIVAKAWAAGFA
                     AVVAVSAPSALAVDTARAAGLQLAGFARDGQINLYAPEDMA"
BASE COUNT          304 a          742 c          846 g          301 t
ORIGIN      
        1 ccgccccgcg gggtgaggtg acgaggacgc gttcgccgtc gaccacaccc aggcgagcgg
       61 cgtcgaccgg gttcacgtcc agggcctcgc cgtagcggat gggggagtcg aagccgccgg
      121 actgcacgcc ggtgttgaac gagtcgaggg cgcggcccgt ggtcaggcgc agcgggaact
      181 cctcgctcag ctcctcgacc ggcccggcgt gctcgaccac gctgaagggg gccggatccc
      241 ggccaccgag atcgggctcc cagagccagc cgtggaggaa ggggctgccg gggtggtcct
      301 cggtcgggca gggccactgc agcccgccct cggcctccag ccgggcgtag gtcatgccct
      361 ggtgcattgg ggagagcgag cgcagctcgt cccagaggtc ctcgggcgtc gggtcacccc
      421 aggcgtagcc catgcgccgg gccagctcgc cgaggatctc gtggtcgtgg cgggcttcgc
      481 ccggtggcgg gacggcggcc cggacccgct ggacccggcg ttcgctggag gtgaccgtgc
      541 cctcgcactc ggcccacgcc accgaggccg ggaggacgac gtcggccaac tcggcggtgc
      601 gggtcaggaa gatgtcctgg acgaccaggt ggtcgagccc ggccagcagg gaccgggcgt
      661 gctcgacgtc ggcctcggag tcggccgggt tctcgccgat cacgtacagg ccggtgagct
      721 cgccccggcc catggcctcg aacatcaggg tcaggtggag gccgggctcg gggttgaggg
      781 gcacgcccca gacggcctcg aaacgggcgc gggccgccgg atcctcgatg tcctggaacc
      841 ccgggagctt gttggggagg gcgcccatgt ccccgccgcc ctgcacgttg ttctggcccc
      901 gcaacgggac caggcccgag ccccaccggc cgacgtggcc ggtgagcagg gccaggttgc
      961 agagcgcgac cacgttgtcg accgcgttgt ggtgctcggt gatgcccagc gtccagagga
     1021 tctgggcctt gtcggcccgg gcgtaggcgt gggccatgtc ccggatggcg tcggcgggca
     1081 gcccggtcag ggccgcggcc gcctcgagcg tgtacggctc gacggcggcg gcgtaggcgt
     1141 cgtagccctg ggtcgagtgg gcgatgaagg cgtcgtcggt caggccggcg gcgatgatct
     1201 cgcggccgag ggcgttggcc agggcgatgt ccgagcccac ctccaggccc agccacacgt
     1261 cggcgaactt ggccgacgac gtacgtcggg ggtccacgca gtagagccgg gccccgttct
     1321 cgatcccctt cagcaggtgg tggaagtaga tcgggtgggc ctcacgggcg ttgctgcccc
     1381 agaggaggac gacgtcggcc tcctccacct cctcgtacga gcaggtccca ccaccagcac
     1441 cgaagactgt cgccagaccg acgacggatg gcgcgtgtca ggttctgttg caggaatcga
     1501 tgtagttgga gcccagggcg gtgcgggcga acttctgcgc cgcgtagttg acctcgttgg
     1561 tggccttcga gcagctgaac agaccgatgc cggtggggcc cttggtgtcc aacacccggc
     1621 ggaagccttc ggtggcccgg tccagggcct cctcccaggt ggccgggcgg agcacaccgt
     1681 cctcgcggac caggggacgt tcgaggcggg ccccgggagc tcgtcgatcg cgtcggttcc
     1741 gaccgggcat cggcgccaag gctaggccat gtcctcgggg gcgtagagat tgatctggcc
     1801 gtcccgggcg aacccggcga gctgcaggcc ggccgcccga gcggtgtcga cggcgagggc
     1861 ggagggggca ctgaccgcga ccacggcggc gaatccggcc gcccaggcct tcgccacgat
     1921 ctcgaacgag gcccgtccgc tgacgaacag gcccaggtcc atggccggca gggccccgtc
     1981 caggagcagc cggccgatca ccttgtccac ggcgttgtga cgaccgatgt cctcccggag
     2041 caggaacggg ctgccgtctc gaccgaaggc ggcggcggcg tggaccgccc cggtggcggc
     2101 gaagagatcc tggtcgacgg ccgccttgtc ggtgacctcc aggaggacct ccggcgggaa
     2161 gggctcgacc gcgggcagcg ggtcgaggcg gtc
//
LOCUS       DASTFV010000681         3706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2606164, whole genome shotgun sequence.
ACCESSION   DASTFV010000681 DASTFV010000000
VERSION     DASTFV010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2606164"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFP02_12495"
     CDS             <1..384
                     /locus_tag="VFP02_12495"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9543902.1"
                     /translation="DRVLLIVGVVAILIDGAAPLGVGILSLLREGLVTVAVIVLAACG
                     AARIDVTWYGKAGTFSVMFAFPLFLWASAGDDTVNDLAYLAAWGFAIPGLILGYIALA
                     QYVPLGLQALRDGRAARAELPEVPA"
     gene            381..2867
                     /locus_tag="VFP02_12500"
     CDS             381..2867
                     /locus_tag="VFP02_12500"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9543903.1"
                     /translation="MKAVIMAGGEGTRLRPLTSNLPKPMMPLANKPMMEHVIDLLRRH
                     GFDEIVVTLAFMPDAIRNHFGDGSEYGVHIQYATEETPLGTAGSVRNAADALDDRFLV
                     ISGDVLTDIDLGEVVAFHEAKGALATIGLVPVENPLEFGIVITNEDGSVERFLEKPSW
                     GQVFSDTINTGIFVLEPAIFDHIADDRAVDFSSEVFPALLAAGLPLFGAVADGYWEDV
                     GTLEAYVSAHKDILDEKVEVDVPGFEVSGGVWVAEGAEIHPDAVIDGPAVVGDNARVE
                     AGARLGEYAVLGTNVKVRAGAEIHRSIVQDNAYLGEGVRLAGAVVGRACDVRRGARCE
                     DGSVLGDECFIGEDAVIGPGVKVYPFKTVEAAAVVNTSIVWESRGARSLFAGLGVSGL
                     ANVDITPEFAARLAMAFGTTLKKDATVVTSRDSSRSARMLKRAMMAGLNSAGVNVSDL
                     EVASIPVTRFQAGRPDNRGGLTVRLVPHDPQQVEVRFFTNSGADLDDDGRRKVERYFT
                     RQDFRRVLPSEIGDIDFPPRALEHYAVALEACIDVRAVRAAEFKVVIDYGYGTMAFVM
                     PNLLSKLGTEVLAVNPYVSTAGILAFERAQHADQVAGLVRTSGARLGAVLDPGGEQLT
                     LIDDTGRVLDDTSTLLVLLALLGDELADRDVALPVATTLHAEALVESHGGRVRPTKLA
                     PSAIMEAAAGDNVVFAAGLDGGYVVPEFLAAFDAAATLVKTLELLARRDVRLSEVVDE
                     LPDQHLVRESVVTPWEQKGTVMRTLMERSGDREVVLVDGVKVRHDGGWVLAWPDPEEP
                     VTNVWAEADSDLAARQLAEEYARRIRQMIR"
     gene            2893..3273
                     /gene="gcvH"
                     /locus_tag="VFP02_12505"
     CDS             2893..3273
                     /gene="gcvH"
                     /locus_tag="VFP02_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET9543904.1"
                     /translation="MNVPEDLRYSKDHEWARLEGDRVRVGITDYAQDALGDVVFVELP
                     EVGSKVKLDESFSEVESTKSVSDVYAPLAGTIAEVNTDLADSPERLNEDPYGEGWICL
                     LEPDDADAYEALMDAAAYRALTEE"
     gene            3287..>3706
                     /locus_tag="VFP02_12510"
     CDS             3287..>3706
                     /locus_tag="VFP02_12510"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9543905.1"
                     /translation="MTCPSCGHENTVGSRFCSSCGTSLGEDQDTTTVTFSPGETGETG
                     ETGDPDAAETPVPEVEVDLSDVPVGTGVLVVTRGEAAGSRILLDLANTSAGRHPKSDI
                     FLDDITVSRRHAEIIREGDQYRVRDAGSLNGTYVNRER"
BASE COUNT          542 a         1300 c         1307 g          557 t
ORIGIN      
        1 gaccgcgtgc tcctcatcgt cggggtggtc gccatcctga tcgacggtgc cgccccgctg
       61 ggggtgggga tcctgtcgct gctgcgggaa ggcctggtca ccgtcgcggt gatcgtcctc
      121 gcggcttgtg gggccgcccg catcgatgtc acgtggtacg gcaaggcggg cacgttctcc
      181 gtgatgttcg ccttccccct gttcctgtgg gcgagcgccg gcgacgacac ggtgaacgat
      241 ctcgcctact tggcggcctg gggcttcgcc atccccgggc tgatcctggg ctacatcgcc
      301 ctcgcccagt acgtccccct cgggctccag gcgcttcgtg acggccgggc cgcccgggcc
      361 gagctccccg aggtgccggc atgaaggcgg tgatcatggc gggcggcgag ggcacccggc
      421 tgcggcccct gacctccaac ctgcccaagc cgatgatgcc gttggccaac aagccgatga
      481 tggagcacgt catcgacctg ctgcgacgtc acggcttcga cgagatcgtc gtcaccctgg
      541 ccttcatgcc cgacgccatc cgcaaccact tcggcgacgg gtccgagtac ggcgtccaca
      601 tccagtacgc caccgaggag accccgctgg gcacggcggg gtcggtgcgc aacgcggccg
      661 atgccctcga cgaccgcttc ctggtcatct ccggcgacgt cctcaccgac atcgacctgg
      721 gcgaggtggt ggcgttccac gaggcgaagg gggcgctggc caccatcggc ctcgtccccg
      781 tcgagaaccc cctcgagttc ggcatcgtga tcaccaacga ggacggctcc gtcgagcgct
      841 tcctcgagaa gccgagctgg ggtcaggtct tctccgacac catcaacacg ggcatcttcg
      901 tgctggagcc ggccatcttc gaccacatcg ccgacgaccg ggccgtcgac ttctcctccg
      961 aggtgttccc cgcgttgctg gccgcgggcc tgcccctctt cggtgcggtg gccgacggct
     1021 attgggagga cgtgggcacc ctcgaggcct acgtcagtgc ccacaaggac atcctcgacg
     1081 agaaggtcga ggtggacgtc cccggcttcg aggtgtccgg cggggtgtgg gtggccgagg
     1141 gggcggagat ccatcccgac gccgtcatcg acggcccggc cgtggtcggc gacaacgccc
     1201 gcgtggaggc aggcgcccgc ctgggcgagt acgccgtcct ggggaccaac gtcaaggtcc
     1261 gcgccggggc ggagatccac cgcagcatcg tgcaggacaa cgcctacctc ggcgaggggg
     1321 tgcgcctggc cggagccgtc gtgggccggg cctgcgacgt ccgccggggc gcccgctgtg
     1381 aggacggttc ggttctgggt gacgaatgct tcatcggcga ggacgcggtc atcgggcccg
     1441 gggtgaaggt gtacccgttc aagacggtcg aggctgcggc ggtcgtcaac acctcgatcg
     1501 tctgggagtc ccgcggcgcg cgcagcctgt tcgccggctt gggggtgagc ggcctggcca
     1561 acgtcgacat cacacccgag ttcgcggcgc gcctggccat ggccttcggc acgacgctga
     1621 agaaggacgc cacggtcgtg accagccgtg actccagccg ctcggcccgc atgctcaagc
     1681 gggcgatgat ggccgggctc aactcggccg gggtcaacgt gtcggacctg gaggtggcgt
     1741 ccatcccggt cacccgcttc caggccggcc gcccggacaa ccggggtggg ctgaccgtgc
     1801 gactggtgcc ccacgacccc cagcaggtgg aggtccgctt cttcaccaac tccggagccg
     1861 acctggacga cgacggtcgg cgcaaagtcg agcgctactt cacccgccag gacttccgac
     1921 gggtcctgcc cagcgagatc ggcgacatcg acttcccgcc gcgggccctg gagcactacg
     1981 ccgtggccct cgaggcctgc atcgacgtcc gggccgtgag ggccgccgag ttcaaggtcg
     2041 tcatcgacta cggctacgga acaatggcgt tcgtgatgcc caacctgttg tccaagctgg
     2101 gtacggaggt gctggcggtc aacccgtacg tgtcgacggc ggggatcctg gccttcgagc
     2161 gggcccagca cgccgatcag gtggcggggc tggtccgcac ctcgggcgcc cgcctcggcg
     2221 cggtgctcga tcccggcggc gagcagctga ccctgatcga cgacaccggc cgggtcctcg
     2281 acgacaccag cacgctgctc gtgctgctcg ccctgctggg cgacgagctg gccgaccggg
     2341 acgtcgccct gcccgtcgcc accaccctcc acgccgaggc cctggtcgag agccacggcg
     2401 gtcgggtccg gccgacgaag ctggcgccgt cggcgatcat ggaggcggcg gcgggtgaca
     2461 acgtggtctt cgccgccggg ttggacggcg gctacgtcgt ccccgagttc ctggctgcct
     2521 tcgacgccgc cgccacgttg gtcaagaccc tcgagctgct ggcccggcgc gacgtccgcc
     2581 tctccgaggt ggtggacgag ctgccggacc agcacctggt ccgggagtcg gtggtcaccc
     2641 cctgggaaca gaaggggacc gtcatgcgca cgctcatgga gcgcagcggc gaccgcgagg
     2701 tcgtgctcgt ggacggggtg aaggtccgtc acgacggggg ctgggtgctg gcctggcccg
     2761 acccggaaga gccggtgacc aacgtctggg ccgaggccga cagcgacctc gccgcccgtc
     2821 agctggccga ggagtacgcc cgccggatcc gccagatgat ccgatgacgc gaacgagggt
     2881 aggttcgcgg cgatgaacgt ccccgaggac ctgcgctact ccaaggacca cgagtgggcc
     2941 cgcctcgagg gtgaccgagt ccgcgtcggc atcaccgact acgcccagga cgccctgggt
     3001 gacgtggtct tcgtcgagct ccccgaggtc gggtcgaagg tgaagctcga cgagagcttc
     3061 agcgaggtcg agtccaccaa gtccgtctcc gacgtctacg ccccgctggc gggcaccatc
     3121 gccgaggtca acacggatct ggccgacagc ccggagcgcc tcaacgagga cccgtacggc
     3181 gagggctgga tctgcctgct ggagccggac gacgccgacg cctacgaggc cctgatggac
     3241 gccgcggcct accgggccct caccgaggag tgacaccgtg tcgggggtga cctgtccgtc
     3301 ctgtggccac gagaacaccg tcgggtcccg tttctgctcg tcctgtggga cctcgttggg
     3361 ggaagaccag gacacgacca ccgtcacgtt cagtcccggt gagaccggtg agaccggtga
     3421 gaccggcgac ccggacgcag ccgagacgcc cgtgcccgag gtcgaggtcg acctgtcgga
     3481 cgtgccggtg ggcacggggg tgctggtcgt gacgcggggc gaggccgccg gctcccgcat
     3541 cctgctggac ctggccaaca cctccgccgg ccgccatccc aagagcgaca tcttcctcga
     3601 tgacatcacg gtgtcccggc gccacgccga gatcatccgc gagggcgacc agtaccgggt
     3661 ccgcgacgcg ggctcgctga acggcaccta cgtcaaccgg gagcgc
//
LOCUS       DASTFV010000682         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2613673, whole genome shotgun sequence.
ACCESSION   DASTFV010000682 DASTFV010000000
VERSION     DASTFV010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2613673"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VFP02_12515"
     CDS             complement(<1..268)
                     /locus_tag="VFP02_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9543906.1"
                     /translation="MTGRLDGRVAVVTGAGDGIGRAIAGAFVAEGARVLVAERDEATG
                     QAAVDALGSEARFLHTDVSRKEDVEAMIADAVATWGTVDVLVNNA"
     gene            complement(307..1836)
                     /locus_tag="VFP02_12520"
     CDS             complement(307..1836)
                     /locus_tag="VFP02_12520"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET9543907.1"
                     /translation="MTDAPPPPGDVAAPRRLGRGVALAIGAVVLLFVVGLAGYALAGR
                     GDDDDGAAAEPTAAPATTTTTTEASEPEDDGQPDPPAHSVGDPYAPDSGATGYDATHY
                     DIAFRYDPEAGTIDGVTTMTAVADVDLDQFSVDLLALDVSEVMVDGEPAEFDVDGRDV
                     RIQPAAPIAEGEEFESEIAYVGVPEPVISAGFPTGWLTTDDGGAYVIGEPDGAATWFP
                     SNDHPSDKATVDLEVTVPRGWTVAGNGDFAGRERVGRDVTWTWQEDDPIATYLVTVAI
                     DHFRVVEEETESGLPLVSFYPEADADRLEGDFADADDMIEAFEEVFGPYPFDEYGAIV
                     VPVDTGLALETQTRSTFGVDVAGIEQFRSHELAHQWFGDSLTPTRWSDIWLSEGFASY
                     AEMLWFDASNPAYDIDVDAENRRDSIVGVDEEPILDPGVDRWFAEAVYQRGGLTLHAL
                     RKTVGDEDFFTILQRWTAEHEHENVTTEEFIALAEEVSGQDLDAFFTDWLEADEIPAL
                     P"
     gene            complement(1833..2015)
                     /locus_tag="VFP02_12525"
     CDS             complement(1833..2015)
                     /locus_tag="VFP02_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543908.1"
                     /translation="MTHINAAPSPDDADPREMGRVVALIIGAVFLLLILGLGALALAS
                     QGDDTSDDRPEVDVSE"
     gene            complement(2012..>2509)
                     /locus_tag="VFP02_12530"
     CDS             complement(2012..>2509)
                     /locus_tag="VFP02_12530"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HET9543909.1"
                     /translation="RYTARALAPRVDSPAELLEVINTALLERGDTRFCTALVARLTPD
                     ADGVDVNLASGGHPPPVLISRQGTRLVDCPGTLLGIYTNASFDEVELRLAPGETLALY
                     TDGVTETRNAQGEMLGEERLVEVLDACVDEHAAKTADQLVQAAVDHAEFAPADDIAIL
                     VIRHL"
BASE COUNT          339 a          854 c          936 g          380 t
ORIGIN      
        1 aggcgttgtt gaccaggacg tcgacggtcc cccaggtggc cacggcgtcg gcgatcatcg
       61 cctccacgtc ctccttgcgg gagacgtcgg tgtggaggaa gcgggcctcg cttcccaggg
      121 cgtcgaccgc ggcctggccg gtggcctcgt cgcgctcggc cacgagaacc ctggcccctt
      181 cggccacgaa ggccccggcg atggcccggc cgatcccgtc cccggcaccg gtgaccaccg
      241 ccacgcgtcc gtccagccgt cccgtcacgt ccacccccgt cctcgaccgg tgcccagcct
      301 cgcgcgtcag ggcagcgcgg ggatctcgtc ggcctccagc caatcggtga agaaggcgtc
      361 gaggtcctgg ccgctgacct cctcggccag ggcgatgaac tcctcggtgg tcacgttctc
      421 gtgttcgtgc tccgccgtcc agcgctggag gatggtgaag aagtcctcgt cgccgaccgt
      481 cttccgcagg gcgtgcaggg tcagcccacc ccgctggtac acggcctcgg cgaaccagcg
      541 gtcgacgccc ggatccagga tcggctcttc gtccaccccg acgatggagt cccgccggtt
      601 ctcggcgtcc acgtcgatgt cgtaggcggg gttcgaggcg tcgaaccaga gcatctcggc
      661 gtagctggcg aagccctcgc tcagccagat gtcggaccag cgggtcgggg tcagcgagtc
      721 gccgaaccac tggtgggcca gctcgtggga ccggaactgc tcgatgccgg cgacgtcgac
      781 gccgaaggtc gagcgggtct gggtctcgag ggccagcccg gtgtccacgg gcacgacgat
      841 ggcgccgtac tcgtcgaacg ggtagggccc gaagacctcc tcgaaggcct cgatcatgtc
      901 gtcggcgtcg gcgaagtccc cctcgaggcg gtcggcatcg gcctcggggt agaagctgac
      961 gaggggcagg ccactctcag tctcctcctc gaccacgcgg aagtggtcga tggcgacggt
     1021 gaccaggtag gtggcgatgg ggtcgtcctc ctgccaggtc caggtcacat cgcggccgac
     1081 ccgctcgcgg cccgcgaagt cgccgttgcc ggccaccgtc cagcctctgg gcacggtcac
     1141 ctccaggtcg accgtggcct tgtccgaggg atggtcgttg ctcgggaacc aggtggcggc
     1201 cccgtccggc tcgccgatga cgtaggcccc gccgtcgtcg gtggtcagcc aacccgtcgg
     1261 gaacccggcg ctgatcaccg gctcgggcac gccgacgtag gcgatctcgg actcgaactc
     1321 ctctccctcg gcgatgggcg cggcgggctg gatgcggaca tcgcgcccgt cgacatcgaa
     1381 ctcagccggt tcgccgtcga ccatcacctc gctgacgtcg agggccagca ggtcgacgga
     1441 gaactggtcg aggtcgacgt cggccaccgc ggtcatcgtg gtcacgccgt cgatcgtccc
     1501 ggcctcgggg tcgtagcgga aggcgatgtc gtaatgggtg gcgtcgtacc cggtggcgcc
     1561 gctgtcgggg gcgtaggggt ccccgaccga gtgggccggg gggtcgggct gaccgtcgtc
     1621 ttcgggctcc gaggcctcgg tcgtggtggt cgtcgtggcc ggcgccgcgg tcggctcggc
     1681 cgcggcaccg tcgtcatcgt cgccgcgacc ggccagggcg tagcccgcca ggccgacgac
     1741 gaagagcagg accaccgcac cgatggcgag ggccacgccc cgacccagcc ggcgaggggc
     1801 ggcaacgtcc cccggcgggg gcggggcgtc ggtcactcgg agacgtcgac ctcggggcgg
     1861 tcgtcagagg tgtcgtcgcc ctggctggcg agggcgaggg caccgaggcc gaggatcagc
     1921 agcaggaaca ccgcgccgat gatgagggcg acgacgcggc ccatctcacg ggggtcggcg
     1981 tcatcgggtg acggagcggc gttgatgtgg gtcataggtg tcggatgacg agtatggcga
     2041 tgtcgtcggc cggtgcgaac tcggcgtggt cgacggcagc ctggacgagc tggtcggccg
     2101 tcttggcggc gtgctcgtcg acgcaggcgt ccagcacctc gaccagccgc tcctcgccga
     2161 gcatctcgcc ttgcgcgttg cgggtctcgg tgacgccgtc ggtgtagagc gccagggtct
     2221 cgcccggggc cagccggagc tcgacctcgt caaagctggc gttggtgtag atgccgagca
     2281 acgtgcccgg gcagtcgacc aggcgtgtgc cctggcggct gatcaggaca ggcgggggat
     2341 ggccaccgct cgccaggttg acgtcgaccc cgtcggcgtc cggggtgagc ctggcgacga
     2401 gagccgtgca gaagcgggtg tcaccgcgct ccaggagagc ggtgttgatc acctccagca
     2461 gctcggccgg gctgtcgacc cggggggcga gggcgcgggc cgtgtagcg
//
LOCUS       DASTFV010000683         5055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2624552, whole genome shotgun sequence.
ACCESSION   DASTFV010000683 DASTFV010000000
VERSION     DASTFV010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2624552"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..210)
                     /locus_tag="VFP02_12535"
     CDS             complement(28..210)
                     /locus_tag="VFP02_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543910.1"
                     /translation="MSETSFCTGCGRPADSCEGGCTRTLDPPRFCPVCGRRSRVDVTP
                     NGYESRCRDHGTFQKG"
     gene            complement(207..2150)
                     /locus_tag="VFP02_12540"
     CDS             complement(207..2150)
                     /locus_tag="VFP02_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9543911.1"
                     /translation="MLRIGGLRSSPILLAVLLVAGTAACSSDDDEAAPATTSPLVPEA
                     GEPVTVGDLELTLCEDLEDIWCGTIEVPQDREDDSSDETLTVGFEWYPRADPGPAEGR
                     LVAMEGGPGYATTASRDYYTELYAPVREHRDLLLMDLRGTGRSEPVDCPDLQAYTGDY
                     SEDTGACGRLLGDEADDYGTVQGADDLSELLLALDAPDDVTLYGDSYGSWFSQIFALR
                     HGDQLGSLILDGTYVVQDMDPWYPTAAEVIRDQLDGQGTLTRLTQAVRAQPLEGRADS
                     YDGTPVDVVLGPSELGDLAASGAFNRAVYRDLDAAGRAWLDDGDPLPLLRLAAENYDP
                     GTEYPLVESSEGEYASVTCNDYDQLYDMASPPAERPEQFDQAIEDYTADAPDAFAPFT
                     VEEWATSPASAYQDCIEWPAIPGDGPVLDPELPYPDVPTLILSGALDSITPIGEAELV
                     AAEMPDATLVQVGASTHVTALGDLFDCASVIAQRFVVDHDAGDTSCAGAIPPLPRVSA
                     FPRRVADAVPATTLSDGASEADRRVVAVTVATVSDALARLRELYTTVGYGLRGGRYRS
                     NGEGTRVVLTDVRFTEDLAVSGSVDANDEDRLQEGSTVSVDVTLDDGTTAELSWTLGG
                     LGDAVILTGTVDGRDVEAQARYA"
     gene            2226..4583
                     /locus_tag="VFP02_12545"
     CDS             2226..4583
                     /locus_tag="VFP02_12545"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET9543912.1"
                     /translation="MATSSRTRPSNASRSRTSSSRRGKGTAKKPAARKPAARRQPAPS
                     KVGLAVSDRGRDLAGVLLIACGVLGGLGIYGGLAGPAGNGIDTATGAVIGLVRWFVPP
                     ALIVAGLLLLRNAPEEDAPGSDDPLLGVRLALAAALLTVAVTGLLHLGQGEPPWGSPT
                     ADFVAAGGVLGAAVARPLSAALDTSGAVLALLTIGVLGFVLGTRASVHALTRKAGVGV
                     QPLARGLREGLGSLFHVGAAASTPEPTVDEGGLFDQDADADDEPMVLGDDDDDEVLDL
                     TEPIPDQPAIVLDDSGAARHAPRGTWKLPPARLLERMGKQDVDRRLVEETGRRLEAAL
                     AEHDVQTRLVGMVVGPTVTRFELELGPGVKVARVTSLHKDIAYAMASPDVRILAPIPG
                     KQAIGVEVPNIKREVVALGDLMAAPETRSATAPLAVPMGRDIGGRTLVADLAGMPHIL
                     IAGATGAGKSSCINSIITSVLMRSTPDEVRMILVDPKMVELGQYDRLPHLLTQVVVDP
                     KKAANALAWATREMDRRYEVLHKVGVRDLGGYNTAVDEDRLDTDVNDQPYERLPYILV
                     VIDELNDLMMVAARDVEDSICRIAQKARAVGIHLVIATQRPSVNVITGVIKANIPARL
                     AFAVSSLADSRVILDQGGAERLVGQGDMLLVDANSSIPHRLQGAWVTEAEVSGVVGHW
                     RRQSPEVHYVEGVEGDTSGGHGSSGGGGDDDDDLLLAAMDLVVRSQLGSTSMLQRKLR
                     VGFARAGRIMDLLEQRGVVGPSEGSKARAVLMTETELNEIQGNAE"
     gene            4822..>5055
                     /locus_tag="VFP02_12550"
     CDS             4822..>5055
                     /locus_tag="VFP02_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET9543913.1"
                     /translation="MGSRFWVETLGCPKNQVDSDKLVGVLEADGYAAADDPTDADLLV
                     INTCAFVEEARQESIDTILAFSDRRAPGAKLVVT"
BASE COUNT          700 a         1800 c         1850 g          705 t
ORIGIN      
        1 cctctccaag gtgatttgag gaggcgttca gcccttctgg aacgtcccgt ggtcccggca
       61 gcgggactcg tagccgttgg gggtcacatc gacgcgggaa cgtcggccgc agaccgggca
      121 gaagcggggc gggtcgaggg tcctcgtgca gccgccctca cacgaatcgg ccgggcggcc
      181 acagcccgtg cagaacgacg tctcgctcac gcgtagcggg cttgggcttc gacgtcgcgg
      241 ccgtcgacgg tgccggtgag gatgacggcg tcgccgaggc cacccagggt ccaggagagc
      301 tcggccgtcg tgccgtcgtc gagggtgaca tcgaccgaaa ccgtcgagcc ctcctggagc
      361 cggtcctcgt cgttggcgtc gaccgagccg gagacggcga ggtcctcggt gaaccggacg
      421 tcggtgagca cgacccgggt gccctcgccg ttggaccggt agcggccgcc gcggaggccg
      481 tagccgacgg tggtgtagag ctcgcggagg cgggccagcg cgtccgacac cgtggccacg
      541 gtcacggcga cgacccgccg gtccgcctcg gacgccccgt ccgacaacgt cgtggcgggc
      601 acggcatcgg ccacacggcg cgggaaggcc gacacccggg gcaggggtgg aatcgccccg
      661 gcgcaggacg tgtcgccggc gtcgtggtcg acgacgaagc gctgggcgat gaccgaggcg
      721 cagtcgaaga ggtcgcccag ggccgtcacg tgggtggagg ccccgacctg gacgagcgtg
      781 gcatcgggca tctcggccgc caccagctct gcctcgccga tcggtgtgat cgagtcgagg
      841 gcgccggaca ggatcagcgt cggcacgtcc ggatagggca gctccggatc caggacgggg
      901 ccatccccgg gaatggccgg ccactcgatg cagtcctggt acgccgaggc gggactggtg
      961 gcccactcct cgacggtgaa cggggcgaag gcgtcggggg cgtcggccgt gtagtcctcg
     1021 atggcctggt cgaactgctc ggggcgctcg gcgggcggcg acgccatgtc gtagagctgg
     1081 tcgtagtcgt tgcaggtgac cgaggcgtac tcgccctcgg aggactcgac cagcgggtac
     1141 tcggtacccg ggtcgtagtt ctcggcggcc agccggagca gggggagcgg gtcgccgtcg
     1201 tccagccagg cccgaccggc ggcgtcgagg tcccggtaga cggcccgatt gaaggcgccc
     1261 gaggcggcca ggtctccgag ctcgctcggg ccgaggacca cgtccaccgg ggtgccgtcg
     1321 tagctgtcgg cccgaccctc caacggctgc gcccggaccg cctgggtcag ccgggtcagc
     1381 gtgccctgac cgtcgagctg gtcccggatg acctcggccg cggtcgggta ccacgggtcc
     1441 atgtcctgca cgacgtaggt cccgtcgagg atgagcgagc cgagctggtc gccgtggcgg
     1501 agggcgaaga tctggctgaa ccacgaaccg tacgagtccc cgtagagggt gacgtcgtcg
     1561 ggggcgtcga gggcgagcag cagctcggac aggtcgtccg ccccttggac cgtgccgtag
     1621 tcgtcggctt cgtcgcccag gagccgcccg caggcacccg tgtcctcgct gtagtcgccg
     1681 gtgtaggcct gcaggtccgg gcagtcgacg ggctcggagc gcccggtacc ccggaggtcc
     1741 atgaggagga ggtcccggtg ctcgcggacc ggggcgtaga gctcggtgta gtagtcccgg
     1801 ctggcggtgg tggcgtaacc cgggccgccc tccatggcca ccaaccgccc ctccgccgga
     1861 ccgggatcgg cgcgcggata ccactcgaag ccgacggtca gggtctcgtc cgacgagtcg
     1921 tcctcgcggt cctggggcac ctcgatcgtg ccgcaccaga tgtcctcgag gtcctcgcac
     1981 agcgtcagct cgagatcacc caccgtgacc ggttcaccgg cctccgggac gagcggcgac
     2041 gtcgtcgcgg gcgcggcctc gtcgtcgtcg gacgaacagg ccgccgtccc ggcgaccagg
     2101 aggacggcga ggaggatcgg ggaggagcgc aggcccccga ttcgtagcat ctccgccccg
     2161 tggtcgcccg ctcacccttg acctcccgtg tggctggtgg ggctgctgtt agcgttgggg
     2221 ccactgtggc tacctcctcg cgcacccgcc cgtcgaacgc ctcgcggtcc cggacctcgt
     2281 cgtcccgccg cggcaagggg acggcgaaga agccggcggc ccgcaagccg gcggcccggc
     2341 gccagccggc accgagcaag gtcggcctgg cggtgagcga ccggggccgt gacctggccg
     2401 gcgtcctgct cattgcctgc ggcgtgctgg gcggcctcgg catctacggc ggtctggcgg
     2461 ggcccgcggg caacggcatc gacaccgcca ccggcgccgt catcggcctg gtccgctggt
     2521 tcgtgccccc cgccctgatc gtcgccggtc tcctcctgct gcgcaacgct ccagaggagg
     2581 acgccccggg gtcggacgac ccgctgctcg gcgtccgcct cgcccttgcc gccgcgctcc
     2641 tcaccgtggc cgtcaccggc ctgctccacc tgggccaggg cgagccgccc tggggttcgc
     2701 cgacggccga cttcgtcgcg gcggggggtg tgctcggcgc cgccgtggcc cgacctctgt
     2761 ccgcggccct cgacacctcg ggggcggtcc tggccctcct caccatcggc gtcctcggct
     2821 tcgtgctggg tacccgggcg tcggtccacg ccctcacccg caaggccggc gtgggtgtgc
     2881 agcccctggc ccggggcctg cgcgaagggc tgggttcgct gttccacgtc ggcgccgccg
     2941 cctcgacccc cgagcccacg gtcgacgaag gcggtctgtt cgaccaggac gccgacgctg
     3001 acgacgagcc catggtcctg ggtgacgacg acgacgacga ggtcctggac ctgaccgaac
     3061 cgatcccgga ccagcccgcg atcgtcctcg acgacagcgg cgcggcgcgc cacgcccccc
     3121 ggggcacgtg gaagctgccg cccgcccgcc tgctggagcg catgggcaag caggacgtgg
     3181 accgccggct ggtcgaggag accggtcggc gcctcgaggc ggccctggcc gagcacgacg
     3241 tccagacccg cctggtcggc atggtcgtcg gacccaccgt cacccgcttc gagctggagc
     3301 tgggcccggg cgtgaaggtg gcccgggtca ccagcctgca caaggacatc gcctacgcga
     3361 tggccagtcc cgacgtccgc atcctggcgc ccatcccggg caagcaggcc ataggtgtcg
     3421 aggtccccaa catcaagcgg gaggtcgtgg cgctcgggga cctgatggcc gcaccggaga
     3481 cccggtcggc cacggccccc ctggccgtgc ccatgggccg tgacatcggc ggtcgcaccc
     3541 tggtggccga cctggccggg atgccccaca tcctcatcgc gggggcgacc ggcgccggta
     3601 agtcgtcctg catcaactcg atcatcacgt cggtgctcat gcgctccacg cccgacgagg
     3661 tccgcatgat cctggtcgac cccaagatgg tcgagctggg ccagtacgac cggttgcccc
     3721 acctcctgac ccaggtggtg gtcgacccca agaaggcggc caatgcactg gcgtgggcca
     3781 cccgcgagat ggaccggcgc tacgaggtcc tgcacaaggt cggcgtccgt gacctcgggg
     3841 gttacaacac cgccgtcgac gaggatcggc tggacaccga cgtcaacgac cagccctacg
     3901 agcgcctgcc ctacatcctg gtcgtgatcg acgagctcaa cgacctgatg atggtggccg
     3961 cccgcgacgt cgaggactcc atctgccgca tcgcccagaa ggcccgggcc gtcggcatcc
     4021 acctggtcat cgccacgcag cgcccgtcgg tcaacgtgat caccggggtc atcaaggcca
     4081 acatcccggc ccgcctggcc ttcgcggtgt ccagcctcgc cgacagccgg gtcatcctcg
     4141 accagggcgg ggccgaacgc ctcgtcggcc agggcgacat gctgctggtc gacgccaaca
     4201 gcagcatccc gcaccggctg cagggggcct gggtcaccga ggccgaggtc agcggcgtgg
     4261 tcggacactg gcgccgccag tccccggagg tccactacgt cgagggcgtg gagggcgaca
     4321 ccagcggcgg tcacggcagc tccggcggcg ggggcgacga cgatgacgac ctcctgctcg
     4381 ccgcgatgga cctggtcgtg cgctcccagc tcggctccac ctcgatgctg cagcgcaagc
     4441 tgcgggtggg cttcgcccgg gccgggcgga tcatggacct gctcgagcag cggggcgtgg
     4501 tcgggccgtc ggagggctcc aaggcccgcg ccgtgctcat gaccgagacc gagctgaacg
     4561 agatccaggg caacgccgag tagcgaggcc tggacccggg cgggatcagc tccgggtggc
     4621 ggtcccctcg acggtggcgt cgccggtcac attgccgcag gagaccgtcg aaggtggagc
     4681 cgtcggcccg gcccgcggcg gtgatctcac cgtggatggt gcccacctcg gtgatctcgg
     4741 tggccacgcc gtcgacggcg tcggagacgg tccaggcacg gtcgggccca ccgtcagggg
     4801 gcgccggtag cctgctgggt catggggtcc cggttctggg tggaaaccct cggttgtccc
     4861 aagaaccagg tcgattccga caagctggtg ggggtgctcg aggctgacgg ctatgccgcg
     4921 gccgacgacc cgaccgacgc cgacctgttg gtcatcaaca cctgtgcctt cgtcgaggag
     4981 gcccggcagg agtccatcga cacgatcctc gccttctcgg accgacgggc gcccggggcc
     5041 aagctggtcg tgacc
//
LOCUS       DASTFV010000684         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2633846, whole genome shotgun sequence.
ACCESSION   DASTFV010000684 DASTFV010000000
VERSION     DASTFV010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2633846"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..788)
                     /locus_tag="VFP02_12555"
     CDS             complement(111..788)
                     /locus_tag="VFP02_12555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9543914.1"
                     /translation="MDHRSRLRPTAGPGQRGRALPRLIRRSHYRRHIVPAISDEALVV
                     AALEGDDKALDALLRRHHDRIYALCRRLAGNDADAADATQEAMITVVRRLDRYDGRSR
                     FTTWLYRVVTNACLDELRRRKRRPVPMADDGLAFDRAPSAPSLDGAVADRLDIDRALA
                     QLAPEFRAPVVLRDLCGLDYAEIGEVLDLPPGTVRSRISRGRGALAHVLAGNPTPSVK
                     RPTREPS"
     gene            complement(719..2368)
                     /locus_tag="VFP02_12560"
     CDS             complement(719..2368)
                     /locus_tag="VFP02_12560"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9543915.1"
                     /translation="MADPPTSPPTQRSDRNGTLLAGRYRFERRIAVGGMAEVWEAVDQ
                     VLDRPVAIKVLHPHLAGDQNFVDRFRREAIAAARLTHPSIVAIYDTHSSDGDEAIVME
                     LVRGISLRKHLDDHGPLPAGEAVDIGARVAEALESAHRAGLVHRDIKPANILLCDDKR
                     VMVADFGIAKLTDADHTSEGTVLGTAKYLAPEQVSGTAVDARTDLYSLGIVLYEMVCG
                     RVPFSGDTDTATVLARLHQDPTPPREIRAEIPRSLEAVILHSLRRDPDDRPASAAALR
                     TELLAALSPGLGGTETINDDPTVLESTTPADGTSVVPPPPPARAAAPPPPTGPPAGRA
                     RAVTPPPPTGRPAPTAKPARNWTGPALLVGLILVALAVAGVLLGSVLGDGLFGEDDPS
                     VPGVDTGDGGGGGDALALTATAFDPEGDGTEHDNEAPLAVDGDVATAWTSEEYNSRAD
                     FQGIKSGVGLVLTLDPAAELGDLGVDAEGTDWSAQVFVADAPAGDLAGWGEPVAEQTG
                     IDGTASFDLAGQSGGAVLVWITDLGSGPPQAQVNEVGLSPG"
     gene            2519..>2985
                     /locus_tag="VFP02_12565"
     CDS             2519..>2985
                     /locus_tag="VFP02_12565"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET9543916.1"
                     /translation="MTLAVVGALAVPTAAPAQAQTTHRLTLDAAQTTVVTDQGGHHVD
                     GHGGAGSTEFGTSGTVWPDPDLKFGFVNHTADVSVEAQESAVAAALATWASVAPFTFT
                     LVPDCGLAFNAAGCTTPDIRVSFGSGDHGAGPDDDDFDGPGGTAAHAYFPPPNG"
BASE COUNT          381 a         1023 c         1179 g          402 t
ORIGIN      
        1 cagccgggcg gacagcacgg ggtcggctgc gacccgggcg cgctcgccct cggtggcctc
       61 gccgtcgagg acggcactga cgagatcgtc gtcggggcgg aatggttcgg tcatgacggc
      121 tcacgggtag gacgcttgac ggagggggtg gggttcccgg ccaggacatg ggccagcgcg
      181 ccccgtcccc gggagatgcg ggagcggacg gtgccaggcg gcaggtcgag gacctcaccg
      241 atctcggcgt agtcgagccc gcacaggtcc cggaggacca ccggggcccg gaactcgggc
      301 gccagctggg caagggcgcg gtcgatgtcc aggcggtcgg cgacggcccc gtccaggctc
      361 ggcgccgaag gcgcccggtc gaaggcgagg ccgtcgtcgg ccatgggcac cggtcgccgc
      421 ttgcgccgcc gcagctcgtc gaggcaggcg ttggtgacga cgcggtagag ccacgtggtg
      481 aagcgggacc ggccgtcgta gcggtcgagg cgacgcacga cggtgatcat cgcctcctgc
      541 gtggcgtcgg cggcgtcggc gtcgttgccg gccaggcgac ggcagagggc gtagatccgg
      601 tcgtggtgcc ggcggagcag ggcatcgagg gccttgtcgt caccctccag ggcggcgacg
      661 accagcgcct cgtcggagat cgcaggaacg atgtggcgac ggtaatggga acggcggatc
      721 agccggggga gagcccgacc tcgttgacct gggcctgcgg tgggccggag ccgagatcgg
      781 tgatccagac caggacggcc ccgccgcttt gcccggcgag gtcgaaggag gcagtcccgt
      841 cgatgccggt ctgctcggcc acgggctccc cccagccggc caggtcgccg gccggcgcgt
      901 cggcgacgaa gacctgcgcc gaccagtcgg ttccctcggc gtccacgccg aggtccccca
      961 gctcggcagc tgggtccaac gtgaggacca gtccgacacc ggacttgatg ccctggaagt
     1021 ccgcccggct gttgtactcc tcggaggtcc aggccgtggc cacgtccccg tcgacggcga
     1081 ggggggcttc gttgtcgtgc tcggtgccgt cgccttcggg gtcgaaggcg gtggcggtga
     1141 gcgccagcgc gtcaccgccg cccccaccgt caccggtgtc gacgccgggg acggacgggt
     1201 cgtcctcgcc gaagaggccg tcgcccagca cgctgcccag caggacgccg gccacggcga
     1261 gcgccaccag gatcaggccg acgagcaggg cggggcccgt ccagttgcgg gccggcttcg
     1321 ccgtcggcgc gggccgtccg gtcggcggtg ggggcgtgac ggcccgggcc cggcccgcgg
     1381 ggggtccggt ggggggcgga ggcgcggccg cccgggccgg gggagggggt gggaccaccg
     1441 acgtgccgtc ggccggcgtg gtcgactcca ggacggtcgg gtcgtcgttg atcgtctcgg
     1501 tgccgcccag gcccggcgac agcgcggcca ggagctcggt tcgcagggcg gcggcggagg
     1561 cggggcggtc gtcggggtcc cgccggaggc tgtgcaggat caccgcttcg agggaccggg
     1621 ggatctcggc ccggatctcc cgaggtgggg tcgggtcctg gtgcaggcgg gccaacaccg
     1681 tggccgtgtc ggtgtcgccg ctgaacggga cccggccgca gaccatctcg tagagcacga
     1741 tcccgaggga gtagaggtcg gttcgggcgt cgacggccgt gccggacacc tgctcgggag
     1801 ccaaatactt ggccgtgccc agcacggtgc cctcgctggt gtggtcggca tcggtcagct
     1861 tggcgatacc gaagtcggcc accatcaccc gcttgtcgtc gcagagcagg atgttggccg
     1921 gcttgatgtc ccggtgcacg aggcccgccc ggtgggccga ctccagcgcc tccgccacac
     1981 gggccccgat gtcgactgcc tccccggccg gcaggggccc gtggtcgtcg aggtgcttgc
     2041 gcagcgagat cccgcggacc agctccatga cgatggcctc gtcaccgtcg ctgctgtggg
     2101 tgtcgtagat ggcgacgatc gaggggtggg tgaggcgggc ggcggcgatg gcctcgcgcc
     2161 ggaagcggtc gacgaagttc tggtcgccgg ccaggtgcgg gtggaggacc ttgatggcca
     2221 ccggccggtc caggacctgg tcgacggcct cccagacctc ggccatcccg ccgacggcga
     2281 ttcggcgctc gaagcggtag cgaccggcga gcagggtccc gttccggtcg gaccgctgtg
     2341 tgggcgggga ggtcggcggg tcggccacgg cggttccacg ctaccgccgc gccggtcccc
     2401 gtcccgggca ggtccgacgg gcgggcgccc gcgggccaaa tgccccctca aggctccgat
     2461 cgggtgggtc gtagacctca cggtggaggc cgggtcggtt cggcgagccg cggccgcggt
     2521 gacgttggcc gtcgtggggg cgttggccgt cccgaccgcg gccccggcgc aagcccagac
     2581 gacccatcgg ctgacgctcg acgccgccca aacgacagtg gtgaccgacc agggcggtca
     2641 ccacgttgat ggtcacggtg gagcaggctc caccgagttc ggcacctccg gcaccgtctg
     2701 gcccgacccg gacctgaagt tcgggttcgt gaaccacacc gcggacgtgt ccgtcgaggc
     2761 ccaggagtcg gcggtggcgg cggccctggc gacgtgggcc tccgtggcgc ccttcacttt
     2821 caccctggtg cccgactgcg gcctggcttt caacgccgcc ggctgcacga ctcccgacat
     2881 ccgggtcagc ttcggatccg gcgaccacgg tgccggacca gacgatgacg acttcgacgg
     2941 cccggggggc acggcggccc acgcctactt cccgcccccg aacgg
//
LOCUS       DASTFV010000685         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2635672, whole genome shotgun sequence.
ACCESSION   DASTFV010000685 DASTFV010000000
VERSION     DASTFV010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2635672"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /gene="ilvN"
                     /locus_tag="VFP02_12570"
     CDS             complement(<1..198)
                     /gene="ilvN"
                     /locus_tag="VFP02_12570"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET9543917.1"
                     /translation="MPALTHTGDARTHTLSVLVENRAGVLTRIAGMFARRGFNIYSLA
                     VAPTDDERFSRITIVVDVESAP"
     gene            complement(228..1958)
                     /locus_tag="VFP02_12575"
                     /pseudo
     CDS             complement(228..1958)
                     /locus_tag="VFP02_12575"
                     /inference="COORDINATES: protein motif:HMM:NF005860.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
BASE COUNT          291 a          666 c          734 g          347 t
ORIGIN      
        1 cggggccgac tcgacgtcga ccacgatggt gatccggctg aagcgctcgt cgtcggtggg
       61 ggcgaccgcc aacgagtaga tgttgaagcc ccggcgggcg aacatcccgg cgatgcgggt
      121 gagcacgccg gcccggttct cgaccaggac cgagagcgtg tgggtgcggg cgtcacccgt
      181 gtgggtcagg gccggcatca gtggccacct tcgccctggg acgggtcgac gatgatcctg
      241 tcgttcgtcg tccccgccgc gaccatcggg tacaccttct cgcgggcgtc ggtccggaag
      301 tcgatgacca ccgggcggtc gtcgatgctg ttggccttct cgatgatcgg cgccacctcc
      361 tcgggtgatt ccgcccgcag gccgacacac cccatggcct cggcccactt cacgtagtcg
      421 ggcaggtccg gcgagaggta gacctcgctg tagcgctcgt cgtagaacat ctcctgccac
      481 tggcggacca tgcccaggta ggcgttgttc aggatcgcca ccttgaccgg gatgcgctcg
      541 gcggcggccg tcaccagctc ctgggccgtc atctggaagc acccgtcgcc gtcgacggcc
      601 cagaccatct tctccgggcg cccgaccttg gccccgatgg cggccggcac cgagaagccc
      661 atggtcccca gcccgccgga gttgacccag gtgtaggggt ggtcgaactt ccagtactgg
      721 ctggtccaca tctggtgctg gcccacgccg gacacgacga tggtgtcgtc gggggtcatg
      781 tcccgcagct cctcgagcac gtactggggc ttgaggccct cgccgtcctc ggccggctgg
      841 taggtcaggg ggtacttctc ctgccagccg ctcagggtgg acttccactc gctgcggtcg
      901 ggctgggact cgagtccgcc ccgacccttc aggacctcca ccagcgcctc gatgaccagg
      961 cggcagtcgc ccacgatcgg gacatccggc cgccggacct tgcccagctc ggcggggtcg
     1021 atgtcgacgt ggatgatctt ggcgtcgggc gcgaagccgt cgagcttgcc cgtgacccgg
     1081 tcgtcgaagc ggctgcccag ggcgatgagc aggtccgacc tctgcatggc cgtgaccgcc
     1141 gtgtagttgc cgtgcatgcc gggcatgccc aggcacagcg ggtggctgtc ggggaaggcc
     1201 ccccgggcca tcagggtggt gaccacgggg atctcgcaga gctcggccag ctccttcagg
     1261 gcctccgcgg cccgggcctt caagatgccg ccaccggcgt agatgaccgg gcggtgcgac
     1321 gcgctgatca actcggccgc ctggcggatc atcttgggat ggcccttggt ggtcgggttg
     1381 taccccggca ggtccagctc gtcgggccag taccactcca tggccgagtt ggggtgctga
     1441 gggtcgacga tgtccttcgg gatgtcgacg aggacgggtc ccggtcgacc cgtggtggcg
     1501 atgtggaagg cctcccggat cgtccgcggg atgtcggccg ccgaggtgac gaggaagttg
     1561 tgcttggtca ccgactgggt gataccggtg gtgtcgcact cctggaaggc gtcgctgccg
     1621 atggccgcgg tcgggacctg gccggtgatg acgaccagcg ggatggagtc catgaaggcg
     1681 tcacacaacg gcgtgacgat gttggtggcc ccggggccac tggtgaccat ggccacgccc
     1741 ggacgcccgg tgacgtgggc gtagccctcg gccatgtggc ccgcgccctg ctcgtggcgg
     1801 accaggatgt ggcggatcgg tgagtcgatg atcgggtcgt acaccggcag gatcgcccca
     1861 cccgggagcc cgaacatgac ctcgacgccc tcgagttcga gggagcggat gagggcctgc
     1921 ccgccgttca gttgttcctt gctgccgttg ctggtcatga gcgcatccga agggtagtgg
     1981 ggccgaaaac tgcaacgacc tccccgtggg gaggtcgaaa gcgcacgcgg gggtgaag
//
LOCUS       DASTFV010000686         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2637375, whole genome shotgun sequence.
ACCESSION   DASTFV010000686 DASTFV010000000
VERSION     DASTFV010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2637375"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VFP02_12580"
     CDS             <1..563
                     /locus_tag="VFP02_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA(Ile)-lysidine synthetase"
                     /protein_id="HET9543918.1"
                     /translation="CSVCGISKRHVFDRAALDGGYDALATGHNLDDEAAVLFGNVLRW
                     ETEYLARQQPVLPARNGFPRKIKPLVRLGERETAAYCILRGIDYEVEECPMAVGNKHL
                     GYKEALNAIETTSPGSKQQFYFGYLERASALFTAGEDDSADGIALSPCANCGAPTPGE
                     VCAFCRLAERAARTQAVAAPVRKAAS"
     gene            563..1336
                     /locus_tag="VFP02_12585"
     CDS             563..1336
                     /locus_tag="VFP02_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HET9543919.1"
                     /translation="MGARPLRAGEQVMLIDGKDRRYLVRLDDGGEFHTHAGIVAHTAI
                     IGADEGALLRANSGVTYRVIRPTLTDFVLKMKRGAQVIYPKDLGPLLLLADIFPGARV
                     LESGVGSGALSMTLLRAGAEIVGYELREDFAARAQTNVGDFLGDEALTRYRVEIRDCY
                     DGIDESDLDRIVLDLPEPWQVVSHAAKALVPGGIFVAYTPSIIQVSQLRQDLDHAGFF
                     SAETVEILQRTWHVDGLAVRPDHRMVAHTGFLTHARAPG"
     gene            1347..>2229
                     /locus_tag="VFP02_12590"
     CDS             1347..>2229
                     /locus_tag="VFP02_12590"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET9543920.1"
                     /translation="MNALDALVVLFAVSAGVAAWRLGFVARSTSWIGMAAGVYVAARI
                     LPAALDRLDSASETQAFVAVVALLVGGGFLGQALGLLVGGRLRVAIPEGSARRVDKTV
                     GALAGVMGVVILLWLTLPTMANVPGWPADLARNSTMARAVNAVLPRPPDTFATLGQFV
                     GDDRFPHVFDALRPAPDLGDPPARTGLPGAVSDRVAASTVRVEGEACSRLQEGSGFAV
                     ATDLVATNAHVVAGNRATAVVRTDGSRAEATVVAFDPDHDLALLSVPGLDRPALALGE
                     ADEGDRGGIYGYPGGGDL"
BASE COUNT          288 a          821 c          786 g          334 t
ORIGIN      
        1 cctgctcggt gtgtggcatc tccaagcgcc acgtcttcga ccgggccgcc ctcgacggcg
       61 gctacgacgc attggccacc ggccacaacc tcgacgacga ggccgcggtc ctgttcggca
      121 acgtcctgcg ctgggagacc gagtatctgg cccgccagca gccggtcctg cccgcccgga
      181 acgggttccc gcgcaagatc aagcccctcg tccgcctagg cgagcgggag accgccgcct
      241 attgcatcct gcggggcatc gactacgagg tcgaggagtg cccgatggcc gtgggcaaca
      301 agcacctcgg ctacaaggaa gccctgaacg ccatcgagac cacctcgccc ggcagcaagc
      361 agcagttcta cttcgggtac ctggagcggg cgtcggccct gttcaccgcg ggcgaggacg
      421 actcggccga cggcatcgcg ctgtcaccgt gcgcgaactg cggggcaccg acgccgggcg
      481 aggtctgcgc cttctgtcgc ctggctgaac gggcagcccg cacccaggcc gtggccgctc
      541 cggtgcgcaa ggccgcctcg tgatgggcgc acgaccactg cgggcgggag agcaggtgat
      601 gctcatcgac ggcaaggacc ggcgctacct ggtccgcttg gatgacggcg gcgagttcca
      661 cacccacgcg gggatcgtgg cccacaccgc catcatcggc gccgacgagg gtgccctgct
      721 ccgggccaac tccggcgtga cctatcgcgt catccggccg acgctgaccg acttcgtgtt
      781 gaagatgaag cggggggcgc aggtcatcta cccgaaggac ctcggtccgc tcctgttgct
      841 ggccgacatc ttcccgggcg cccgggtgct cgagtccggc gtcggctccg gcgccctgtc
      901 catgaccctg cttcgggccg gggccgagat cgtgggctac gagctgcgag aggacttcgc
      961 cgcccgggcc cagaccaacg tcggggactt cctgggcgac gaggctctga cccgctaccg
     1021 ggtcgagatc cgggactgct acgacgggat cgacgagtcc gacctggacc ggatcgtcct
     1081 cgacctcccc gagccctggc aggtggtgtc ccacgcggcc aaggcgctgg tccccggagg
     1141 gatcttcgtg gcctacaccc cgtcgatcat ccaggtcagc caactccgtc aggacctcga
     1201 ccacgccggg ttcttctcgg cggagacggt cgagatcctg cagcgcacct ggcacgtcga
     1261 tggcctcgcc gtccggccgg accaccggat ggtggcccac accgggttcc tgacccacgc
     1321 ccgcgccccc ggctgaccgg gtggggatga acgctctcga cgccctggtc gtgctgttcg
     1381 ccgtgtcggc gggggtggcc gcctggcgcc tgggcttcgt ggcccgctcc acgtcgtgga
     1441 tcggcatggc ggccggggtc tacgtcgcgg cccggatcct gcctgcggcc ttggaccgcc
     1501 tggattcggc cagcgagacc caagccttcg tcgccgtggt cgcgctcctg gtcggcggtg
     1561 gcttcctcgg ccaggccttg ggcctgctcg tcggaggacg gctccgggtg gccatccccg
     1621 agggctccgc tcgtcgggtc gacaagaccg tcggcgcgct ggccggggtg atgggcgtgg
     1681 tcatcctcct ctggctgacg ctgcccacca tggccaacgt gcccggctgg cccgccgacc
     1741 tggcccgcaa ctcgacgatg gcccgggccg tgaacgccgt cctgccccgg ccgcccgaca
     1801 ccttcgccac cctcggccag ttcgtcggcg acgaccgctt cccgcacgtc ttcgacgccc
     1861 tccgaccggc gccggatctc ggcgacccgc cggcccgcac gggcctaccc ggggcggtgt
     1921 cggaccgcgt cgcggcgtcg accgtccgcg tcgagggtga ggcgtgtagc cggctgcagg
     1981 aggggagtgg gttcgccgtg gcgaccgatc tggtcgccac caacgcgcac gtcgtggcgg
     2041 gcaaccgggc caccgcggtg gtgcggacgg acggctcccg ggcggaggcc accgttgtgg
     2101 ccttcgatcc ggaccacgac ctggccctgc tgtccgtacc cgggctcgac cggccggcgc
     2161 tggccctggg tgaggccgac gagggcgacc gcggtggcat ctacgggtac ccgggcggcg
     2221 gggacctgc
//
LOCUS       DASTFV010000687         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2638731, whole genome shotgun sequence.
ACCESSION   DASTFV010000687 DASTFV010000000
VERSION     DASTFV010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2638731"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="VFP02_12595"
     CDS             <1..1112
                     /locus_tag="VFP02_12595"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9543921.1"
                     /translation="PVFAVHRILDPDQAEAVLAEGGADGICLVRALIADPDWVEKAAS
                     GRADEIRRCTGSNQSCYGNLINGMPVSCVQNPAVGREAELGSGTLGTAPVAKKVVVVG
                     GGPAGLEAAWVAAARGHRVVLLEQAEELGGRIRLAAQLPGRSEMAAVADWRAGECERR
                     GVDVRLGVRADAATLLALSPDAVIVATGGRADKGVKAKYHPTPVLGYEQDWVIDHIEA
                     IERALAGSLTGKVVVLDAVGHIEAIALGELLGRQDVDVTVVTALSAPISLDPETQQAA
                     LPRAVRAGMAWRPNTVLGAIGDHEVTLVDALSHAIEQVTGVDWVVIRTHGRPENALVA
                     ELEGKVPEVVAVGDAVAVRPADRAIWDGHQAARAL"
     gene            complement(1178..1642)
                     /locus_tag="VFP02_12600"
     CDS             complement(1178..1642)
                     /locus_tag="VFP02_12600"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HET9543922.1"
                     /translation="MIDHQVNAGEVEIADLYQRFYGFARRGRPGVRKLRTVLEARGPG
                     FVAPESELEHETLELIRRSGLPEPELQVVLAFWESLVGRVDFVYSDARLIVEVDGRRY
                     HGPETFEHDRLRDNAAGLVGWRVLHFTWKMVTQTPEYVVASIREGLRIGRQD"
     gene            1631..2128
                     /locus_tag="VFP02_12605"
     CDS             1631..2128
                     /locus_tag="VFP02_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543923.1"
                     /translation="MVDHPTEPLWAQESSGITDRSFPTGHGDAVHPSTVTFRQVRALV
                     HVRELEAVIRPQGNRDHDLVAPQPDHPPQLCRGLMGCDGAGDRHRRGHQVMGERPRDA
                     GDRQRLSTEPTPATRVQPMLDHPIGGAEAARLRTGEDSVLRLGMAGDGGIDGEIHDAQ
                     DAERV"
BASE COUNT          357 a          824 c          872 g          340 t
ORIGIN      
        1 cgcccgtctt cgccgtgcac cgcatcctgg accccgacca ggccgaggcc gtcctggccg
       61 agggcggggc cgacgggatc tgcctggtgc gggccctgat cgccgacccg gactgggtgg
      121 agaaggcggc gtccggccgg gccgacgaga tccggcgctg caccgggtcg aaccagtcct
      181 gctacgggaa cctgatcaac ggcatgcccg tctcgtgcgt gcagaacccg gcggtgggcc
      241 gcgaggccga gctggggtcg gggacgctcg ggaccgcgcc ggtggccaag aaggtcgtcg
      301 tggtcggagg cggccccgcc gggctggagg cggcctgggt ggccgcggcc cggggccatc
      361 gggtcgtgct gctggaacag gccgaggagc tcggcggccg gatccggctg gcggcccagc
      421 tccccggtcg ctccgagatg gcggcggtgg ccgactggcg ggcgggcgag tgcgagcgtc
      481 gaggggtcga tgtccgcctg ggcgtgcggg ccgacgccgc cacgctcctg gcgctgagcc
      541 ctgacgccgt gatcgtggcc accggcggcc gggccgacaa gggggtcaag gccaagtacc
      601 acccgacgcc ggtgctcggt tacgagcagg actgggtcat cgaccacatc gaggccatcg
      661 agcgggcgct ggccggctcg ttgaccggca aggtggtcgt gctcgacgcg gtgggccaca
      721 tcgaggcgat cgccctgggg gaactgctgg gtcgccagga cgtggacgtc acggtggtca
      781 ccgccctgtc ggccccgatc tccctcgacc ccgagaccca acaggccgcg ctcccccgcg
      841 ccgtccgagc cggcatggcc tggcgcccca acacggtcct cggcgccatc ggcgaccacg
      901 aggtcaccct ggtcgacgct ctctcccacg ccatcgagca ggtcaccggc gtggactggg
      961 tcgtcatccg cacccacggc cgccccgaga acgccctggt ggccgagctg gagggcaagg
     1021 tccccgaggt ggtggccgtg ggcgacgccg tggccgtccg ccccgccgac cgcgccatct
     1081 gggacggcca ccaggccgct cgcgccctct gacccacccc tcggcccctc cgaactttcg
     1141 gcagaactcg accccggatg ggacctttgc cgacggttca gtcctggcgg ccgatgcgga
     1201 ggccctcgcg gatggaggcg accacgtact ccggtgtctg cgtcaccatc ttccacgtga
     1261 agtggaggac acgccagccg accagaccgg ccgcgttgtc ccgtaggcgg tcgtgctcga
     1321 aggtctcagg gccgtggtac ctacgcccgt cgacctcgac gatgaggcgt gcatcggaat
     1381 agacgaagtc cacgcgaccg acgagcgact cccagaaggc gaggacgacc tggagctccg
     1441 gctccggaag acccgaacgt cggatgagct cgagtgtctc gtgttcgagc tccgactccg
     1501 gggcgacgaa gccaggacca cgagcctcga gcaccgtccg gagcttcctg accccgggtc
     1561 gaccccgtcg ggcgaagccg tagaagcgtt gatagaggtc ggcgatctcg acctcgcccg
     1621 cgtttacctg gtggtcgatc atccgaccga gccgctctgg gcgcaggagt cgagcggcat
     1681 cacagatcgt tcgttcccga caggtcacgg cgatgccgtc cacccgtcga cggtcacgtt
     1741 caggcaggtc cgtgctctcg tgcacgttcg cgaactcgag gcggtgattc gcccccaagg
     1801 gaaccgtgac cacgatctgg tcgcgccgca gcccgaccat cccccacaac tgtgccgcgg
     1861 actcatggga tgcgatggcg ccggggatcg acaccgtcgc ggccatcagg tcatgggcga
     1921 acgacctcgg gacgccggcg accgccagcg cctctcgact gaacccacgc cagcgacccg
     1981 tgtccagccg atgctggatc acccgatcgg tggcgccgag gccgcgcgct tgcgtacggg
     2041 tgaagattcc gtgctgcgtc tcggcatggc gggcgacggc ggcatcgatg gtgagatcca
     2101 tgacgcacaa gatgccgaaa gggtgtgaca ctgaacgacg tgatccacac cttcggcagg
     2161 agtgaccgag gacgtcgttc tttgccgaaa gcttgggggg ccgcgccaac cttcggcgtc
     2221 ggtcacaagg caggtcgagt tctgccgaaa ggttctcagg cggcgggggt ggctctggcg
     2281 gcgacgtcct ggcaggccgc caggaggggt ggggcgaagg cggggcgcat gcaggcgatg
     2341 tggccgtcgg cgtgctcgtg gacggtggcg ccggggatgg cgtcggccat ctc
//
LOCUS       DASTFV010000688         3292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2642569, whole genome shotgun sequence.
ACCESSION   DASTFV010000688 DASTFV010000000
VERSION     DASTFV010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2642569"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="VFP02_12610"
     CDS             <1..1092
                     /locus_tag="VFP02_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9543924.1"
                     /translation="LGAQFDAGLAWVHFPAGRGGLGLEPSAQRPVNEALRAAGAPEAY
                     WQNPIGHGMGAPTVVTHGSDGQRDRYLRPLFTGEEVWCQLFSEPGAGSDVAGLSGRAV
                     RDGDEWVVNAQKVWTTLAHLARWGMLLVRTDPEQPKHKGLTYFVVDMQAPGVDVRPLR
                     QITGEAEFNEVFFTDVRIPDAERLGDVGEGWGVSMTTLMNERVAIGGSVTPKGSGPIG
                     EALRLWNQRADKDPVTRDRLTRLWIEAEVNRLTNLRAQANRKVGTPGPEGSVGKLAFA
                     EANKAIYAFCLDLLGPEGMLYGSYEMTRPEHAMFSDSTQKSFLRARANSIEGGTSEIM
                     RNILGERVLGLPGDVRVDKDRPWSDVPRS"
     gene            complement(1105..1698)
                     /locus_tag="VFP02_12615"
     CDS             complement(1105..1698)
                     /locus_tag="VFP02_12615"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9543925.1"
                     /translation="MDLLLIRHALPVRIDDGEGPADPELSDEGWRQAGALAAWLADEE
                     IEAMVASPLRRAQETAEPLATLLDLEVTTVDGLAEFDRDAPWYIPMEELKAADDPRWH
                     AMVNGEFHLDPDEFQKGVVEAVDGVIDAHPGLTVAAVCHGGTINAYLSAMLGIDRLMF
                     FFPDYTSVSRVRASRSGARGLVSINETSHLRFAKRST"
     gene            1758..3188
                     /gene="pyk"
                     /locus_tag="VFP02_12620"
     CDS             1758..3188
                     /gene="pyk"
                     /locus_tag="VFP02_12620"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET9543926.1"
                     /translation="MRRRTKIIATIGPASDSEATLVALVEAGMDVARIGLAHGDLDHH
                     LECYDRIRKASVEADRPVGILVDLPGPKVRAGSFPESGVELVEGERLRLTPGFSASTA
                     EVVEVGYERLVSDVEVGDRMLFGDGAVVIEVVDKNDRDLIATVCHGGKVQGRPGVHIP
                     SDRLRIASPTPDDLRMLDAFVERGVDMVAVSFVRSAHDIRRVGTEPHPRGPLVVAKIE
                     TRAAVQNLEGIIQASGAVMVARGDLGAEMPIAEIPHLQKHIIRTCIALGRPAITATQM
                     LESMVHAGTPTRAEVTDVANAVFDGTSVVMLSGETAIGHDPVLVVRTMADIARRADEE
                     FDYDGWAPHITDLVHTEEGDADARVTDTMTMAAWRVAKEMRADAILCVSRTGFTVRAM
                     ARFRPKTKILGFSPDERTVRQLTMSWGAQPMLLEQRGSNESMVRAAVRAAREAGQVRS
                     GDTVVVLAGSDDRSRATDVLRLVRVP"
BASE COUNT          484 a         1198 c         1149 g          461 t
ORIGIN      
        1 ctgggcgccc agttcgacgc cggcctggcg tgggtccact tccccgcggg tcgaggtgga
       61 ctcggcctcg agccgtcggc ccaacgaccg gtcaacgagg ccctccgggc ggcgggcgcc
      121 cccgaggcgt actggcagaa ccccatcggc cacgggatgg gagcccccac ggtcgtgacc
      181 cacggcagcg acgggcaacg ggaccgctac ctgcgccccc tcttcaccgg tgaagaggtc
      241 tggtgtcagc tcttcagcga gccgggggcc ggttcggacg tggccgggct gtcggggcga
      301 gccgtgcgcg acggtgacga atgggtcgtc aacgcgcaga aggtgtggac cacgctcgcc
      361 cacctggccc gctgggggat gctcctggtc cgcaccgacc ccgagcagcc caagcacaag
      421 ggcctgacct acttcgtggt cgacatgcag gctccggggg tcgacgtgcg cccgctgcgc
      481 cagatcacgg gcgaggccga gttcaacgag gtgttcttca ccgacgtccg catccccgac
      541 gccgagcgcc tcggcgacgt cggcgagggc tggggggtgt cgatgaccac cctcatgaac
      601 gagcgggtgg ccatcggcgg gtcggtgacg cccaagggct ccgggccgat cggcgaggcc
      661 ctccgcctgt ggaaccagcg ggccgacaag gatcccgtca cccgggaccg cctcacccgg
      721 ctgtggatcg aggccgaggt caaccgcctg accaacctgc gagcccaggc caaccggaag
      781 gtcggcaccc ccgggccgga gggctcggtg ggcaagctgg ccttcgccga ggccaacaag
      841 gcgatctacg ccttctgcct cgacctgctg gggccggagg ggatgctcta cgggagctac
      901 gagatgaccc gtccggagca cgccatgttc tccgacagca cccagaagtc gttcctgcgg
      961 gcccgggcca actccatcga gggcggcaca agcgagatca tgcgcaacat cctcggtgag
     1021 cgggtcctcg gcctgcccgg tgacgtccgg gtcgacaagg accgcccctg gagcgacgtc
     1081 ccccgcagct gatccccgtc gggatcaggt ggagcgcttg gcgaagcgga ggtggctggt
     1141 ctcgttgatg ctgacgaggc cgcgggctcc cgagcgggac gcccgcaccc ggctgacgct
     1201 ggtgtagtcc gggaagaaga acatcagccg atcgatgccc aacatcgccg acaggtaggc
     1261 gttgatggtc ccaccgtggc agaccgcggc caccgtgagg cccgggtggg cgtcgatcac
     1321 cccgtcgacc gcctcgacca cccccttctg gaactcgtcg gggtccaagt ggaactcgcc
     1381 gttgaccatc gcgtgccacc gggggtcgtc ggccgccttc agctcctcca tggggatgta
     1441 ccacggggcg tcgcggtcga actcggccag gccgtcgact gtggtcacct cgaggtccag
     1501 cagcgtggcc agaggctcgg cggtctcctg cgcccgccgc agcgggctgg cgaccatggc
     1561 ctcgatctcc tcgtcggcca gccaggcggc cagcgcgccg gcctggcgcc agccctcgtc
     1621 actcagctcc gggtccgccg ggccctcgcc gtcgtcgatg cggacgggga gcgcgtgacg
     1681 gatcaggagg aggtccatgg cgggcggagc ctacctattg ccccggtggc gaccgttccc
     1741 gtcgctagcg tgagggcgtg cgccgtcgga ccaagatcat cgccacgatc ggtccggctt
     1801 cggactccga ggcgacgctg gtcgccctcg tcgaagcggg catggatgtc gcccgcatag
     1861 ggctggccca cggggacctc gaccaccacc tcgagtgcta cgaccggatc cgcaaggcgt
     1921 cggtcgaagc cgaccgtccc gtcgggatcc tggtcgacct gccggggccc aaggtgcggg
     1981 cgggcagctt ccccgagagc ggcgtcgaac tggtcgaggg tgagcgcctc cgcctcaccc
     2041 cgggcttcag tgccagcacc gccgaggtcg tcgaggtggg ctacgagcgg ctggtcagcg
     2101 acgtcgaggt cggcgaccgg atgctgttcg gcgacggcgc cgtggtcatc gaggtcgtcg
     2161 acaagaacga tcgcgacctc atcgccaccg tctgccacgg cggaaaggtc cagggccgcc
     2221 cgggcgtcca catcccgtcg gaccgcctgc gcatcgcctc acccacgcct gacgacctgc
     2281 gcatgctcga cgcgttcgtg gagcggggcg tggacatggt cgccgtgtcg ttcgtccgct
     2341 ccgcccacga catccgccgc gtcggcaccg agccccatcc ccggggcccc ctggtcgtgg
     2401 ccaagatcga gacccgggcc gccgtccaga acctcgaggg gatcatccag gccagcggcg
     2461 cggtcatggt cgcccgaggc gacctcggcg cggagatgcc catcgccgag atcccgcacc
     2521 tgcagaagca catcatccgc acctgcatcg ctctcggtcg tccggccatc accgccaccc
     2581 agatgctgga gtccatggtc cacgccggga ccccgacccg ggctgaggtc accgatgtgg
     2641 ccaacgccgt cttcgacggc acgagcgtgg tcatgctctc gggcgagacc gcgatcggtc
     2701 acgaccccgt cctggtcgtc cgcaccatgg ccgacatcgc ccgccgggcc gacgaggagt
     2761 tcgactacga cggctgggcc ccgcacatca ccgacctcgt ccacaccgag gagggtgacg
     2821 ccgacgcccg ggtcaccgac accatgacca tggcggcctg gcgggtggcc aaggagatgc
     2881 gcgccgacgc catcctctgc gtgtcccgga cggggttcac cgtgcgggcc atggcccggt
     2941 tccggcccaa gaccaagatc ctgggcttct cgcccgacga gcgcaccgtg cgccagctca
     3001 ccatgagctg gggcgctcag ccgatgctcc tggagcagcg ggggagcaac gagtccatgg
     3061 tccgggccgc cgtgcgcgcc gcccgcgagg ccggtcaggt ccggtccggc gacaccgtgg
     3121 tcgtcctggc cggctccgac gaccgctccc gggccaccga cgtcctccgc ctggtgcggg
     3181 tgccctgagc ccaggcggcc cgtcgacgtc gccatccagt cgcactggct gtcccgtcgg
     3241 ctgcgctacg accgggacct ggctgagctc gaccctcggt gcgagtaggt ga
//
LOCUS       DASTFV010000689         5986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U13
            k141_2642925, whole genome shotgun sequence.
ACCESSION   DASTFV010000689 DASTFV010000000
VERSION     DASTFV010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969887
            Sequence Read Archive: SRR25158457
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 11:01:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,530
            CDSs (total)                      :: 2,512
            Genes (coding)                    :: 2,502
            CDSs (with protein)               :: 2,502
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2642925"
                     /isolate="SMAG_U13"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="Belgium"
                     /lat_lon="50.87 N 4.71 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFP02_12625"
     CDS             complement(<1..347)
                     /locus_tag="VFP02_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543927.1"
                     /translation="MNRLRTWWSNPWRRPAFLATGFWLYVIWSLVPVLIAIQFAFNSG
                     RSRSTWQGFSLRWWFDDPDRSLWHDPSLRDALVNSLWLAFFTMLIATPLGVALALGLA
                     RWRGRTARASNLL"
     gene            complement(344..1258)
                     /locus_tag="VFP02_12630"
     CDS             complement(344..1258)
                     /locus_tag="VFP02_12630"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9543928.1"
                     /translation="MSRAPAGRNRTRTLWPSLALPGVVWLLLFFVVPFYGVLAVAFGG
                     LDPIFGNAVPVWNPLQWDSTVFVDVLDRVVNAELREVYLRTFAYVGTALFLCFLIGYP
                     VAYYVSRFGGRRRGLLLGLIIAPFWINYLMRMLAWINLLQEDGYVNDVLSALHLTSAP
                     VNWLEGRSITVVLGLVYGYIPFFVLPLFAALDRIDRSVMEASRDLGMGPLRTFLRVTL
                     PLSKQGLLAAAVITALPMMGDYYTADLLSGSPRTSMIGNQIEFFLFEGSQKNAGASLV
                     VILSIMLMAGMAYYLVQTDRATRELHRS"
     gene            complement(1269..2528)
                     /locus_tag="VFP02_12635"
     CDS             complement(1269..2528)
                     /locus_tag="VFP02_12635"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9543929.1"
                     /translation="MTFHPRERRIPVGIDRRTFLRRSAFTAAAVAAGPSLLAACGGDN
                     GGGGGGGGEAVQLSRPDNPVTLPTFDDIPMIADGLDPESGPLKIYNYAEYIAPQTLRG
                     FRREYGVDVEVTTFTSMDEAIARLSGGQADFDLFFATTDVIGKATVGRLLQPLNATYL
                     GNSSNVWTQLQDPFYDVGAQYSRPYAMYATGIGYRIDRVSQAPGDYDNPYDIMWDPEY
                     SGDLYLLEDDREVLGMAMLRAGETDVNTEDQAKVDAALADIQELADAVNPRIGVEAYT
                     ILPEGRATIHQAWSGDAVNAQYYLPEGESIENIGFWYPDDGGGMIGSDQLTVLAGAAS
                     PVLAHLFIDYVLDADNALTNFAWLGYQPPQTSIDPDSVVADGLVPPHLESTVIQPEDF
                     ETGYQLLQLSLAGERLWDNAWSTFTAG"
     gene            2616..4238
                     /locus_tag="VFP02_12640"
     CDS             2616..4238
                     /locus_tag="VFP02_12640"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9543930.1"
                     /translation="MSSTYDAIVVGGGHNGLVAAAYLARAGMKPVVLEARDNVGGAAT
                     TETPWGPEFKMTALSYVMSLMPPTILNDLKLAEHGYHVIPMGPTFIALPDGRYLLAED
                     DPELDRDEVAKFSSKDADRLGDYHAWIGGVADVLAPLLLSTPPSVGSRRPKDLMEQLR
                     LAWGLRGLDVRGTADATRLFTMSIRDVLDEWFESPEVQGLMAINAVIGTWAGPDEAGT
                     AYVMMHHTIGDVGDGHLGKWGYPIGGMGAVSDAIRASAESLGAEIRTNAPVERIRTRN
                     GRVIGVALADGTELEADIVVAATHPQITFLRHLDRSELPDDFVHDLEHWRSRSGTVKV
                     NVALSELPDFDAWPGPPDQEKLSGSVELCHSIDHLQKAFEEARWGKASTLPFSDGVIP
                     STVDPSLCPEGTHVMSLFTQWVPQEWSSEPDHAELEAYADRVIDGYNDLAPNFKASVI
                     HRQVIGPYEMENEYGLIGGNIFHGELSPDQLFHMRPAPGYADFTTPIGGLYQCSSATH
                     GGGGVTGIPGLLCTRRILKDHKRSRLLRRGER"
     gene            4244..5440
                     /locus_tag="VFP02_12645"
     CDS             4244..5440
                     /locus_tag="VFP02_12645"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET9543931.1"
                     /translation="MDLRSAAPVDQAAIEAILAPGAAGRMLPAEAYSSEAVLAWERRH
                     LFAESWVCAGRSADLADAGDRRALRVGDDSVVLVRGDDGVLRAFYNVCRHRGHQLQPE
                     DTTVRRNAIHCPYHAWTYALDGTLKFTPRFDAPEGFDPTQFPLVPVRTEEWCGWVFVN
                     ASGDARPLAEHLGDLDAEMARWDPERLVVGASHSYELAANWKLPIENYHECFHCAAIH
                     PEFCVVSPPNSGDNCADHDGMWIGGWMVLADDAETMAFDGRSEGVMLPGIDETWRRRV
                     HYIGLLPNLLISPHPDYVMTHRFEPLGPDRTFVECQWLFDPAAVNRPGFDPAYAVDLW
                     DLTNRQDWAACEGVQRGVASRGYRPGPFAADEDAVTQFVQLMAQTYRDGALPSGVPFP
                     EQELTR"
     gene            5437..>5986
                     /locus_tag="VFP02_12650"
     CDS             5437..>5986
                     /locus_tag="VFP02_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9543932.1"
                     /translation="MTVTAGTDAELAVIDGLIAEQEAALLDRTPASAAIMDEARGALA
                     GGVASSWQDAPPRAIWIDHGKGSKITDVDGTTYVDLHNGFGAMLVGHAHPAVVKAVQE
                     RVALGTHFAQPTEDLIPVSRDLARRFDQPKWRFANSGTEATLAAFNLMRVVKGRKKVI
                     KIEGSYHGHHDSVQVSVYPDFED"
BASE COUNT          984 a         2146 c         2014 g          842 t
ORIGIN      
        1 atcaagaggt tggacgcccg cgccgtccgc ccccgccagc gggccaggcc cagggccagc
       61 gccaccccga ggggggtggc gatcagcatg gtgaagaagg caagccacaa gctgttgacc
      121 agcgcgtcgc gcagcgacgg gtcgtgccag agcgagcggt ccgggtcgtc gaaccaccag
      181 cgcaacgaga agccctgcca ggtgctgcgg gaccggccgg agttgaaggc gaactggatg
      241 gcgatcagca ccggcaccag cgaccagatg acgtagagcc agaagcccgt ggccaggaag
      301 gcgggccggc gccacgggtt cgaccaccag gtgcggagcc ggttcacgat cggtggagct
      361 cccgagtggc ccggtcggtc tggaccaggt agtaggccat cccggccatc aacatgatcg
      421 acaggatcac gaccagggac gccccggcgt tcttctggga gccctcgaac aggaagaact
      481 cgatctggtt gccgatcatg ctcgtccggg gcgaccccga cagcaggtcc gccgtgtagt
      541 agtcccccat catcggcagg gcggtgatga ctgcggcggc cagcagtccc tgcttggaca
      601 agggaagggt caccctcagg aaggtccgca ggggccccat gccgaggtcc ctcgacgcct
      661 ccatgacgct ccggtcgatg cggtccagcg ccgcgaacag gggcaggacg aagaacggga
      721 tgtagccgta cacgaggccc agcaccacgg tgatggaccg gccctcgagc cagttcaccg
      781 gcgccgaggt caggtgcagg gcgctcagga cgtcgttgac gtatccgtcc tcctggagga
      841 ggttgatcca ggccagcatc cgcatcaggt agttgatcca gaacggggcg atgatcaggc
      901 cgaggagcag acctcgccgc cgcccgccga agcgcgacac gtaataggcc accgggtacc
      961 cgatgaggaa gcagaggaac agcgcggtgc ccacgtaggc gaacgtgcgc agatagacct
     1021 cgcgcagctc ggcgttgacc acccggtcga ggacgtcgac gaagaccgtg ctgtcccact
     1081 ggagcgggtt ccagaccggg acggcgttgc cgaagatcgg gtcgaggccg ccgaaggcca
     1141 ccgccagcac cccgtagaag gggacgacga agaagaggag gagccagacg acccccggca
     1201 gggcgaggct gggccagagg gtccgggtgc ggttccgccc ggcgggcgct cgactcacgc
     1261 ccggccgctc agccggcagt gaaggtcgac cacgcgttgt cccacagccg ctcaccggcc
     1321 agggagagct ggaggagctg gtagcccgtc tcgaagtcct cgggctggat cacggtggac
     1381 tcgaggtggg gcgggaccag gccgtcggcc accaccgagt ccggatcgat gctcgtctgc
     1441 ggcggctggt aacccagcca ggcgaagttg gtgagggcgt tgtcggcgtc gagcacgtag
     1501 tcgatgaaga ggtgagccag caccgggctg gcggcgccgg ccagcaccgt cagctggtcc
     1561 gagccgatca tcccgccgcc gtcatccgga taccagaagc cgatgttctc gatcgactcg
     1621 ccctccggca ggtagtactg cgcgttgacc gcatcccccg accaggcctg gtggatcgtg
     1681 gcccggccct cgggcaggat cgtgtaggcc tcgacaccga tccgcgggtt gacggcgtcg
     1741 gccagctcct ggatgtcggc gagggcggcg tcgaccttgg cctggtcctc ggtgttcacg
     1801 tcggtctccc ccgcccggag catcgccatg cccagcacct cgcggtcgtc ctccagcagg
     1861 tacaggtcac cggagtactc gggatcccac atgatgtcgt acgggttgtc gtagtcgccc
     1921 ggggcctggg acacccggtc gatccggtag ccgatccccg tggcgtacat cgcgtaggga
     1981 cggctgtact gcgccccgac gtcgtagaag gggtcctgga gctgggtcca gacgttcgac
     2041 gagttcccga ggtaggtggc gttcaggggc tgcaggaggc ggcccaccgt ggccttgccg
     2101 atgacatcgg tcgtggcgaa gaagaggtcg aagtcggcct ggcccccgga cagccgggcg
     2161 atggcctcgt ccatgctggt gaaggtcgtc acctcgacgt cgaccccgta ctcccggcgg
     2221 aagccccgga gcgtctgggg ggcgatgtac tcggcgtagt tgtagatctt gagcgggccc
     2281 gactcgggat cgaggccgtc ggcgatcatc gggatgtcgt cgaaggtggg caaggtcacc
     2341 gggttgtcgg gccgggagag ctggacggcc tcgcccccac ctccgccgcc gccaccgttg
     2401 tcgccaccgc acgcggccag cagcgacggg cccgcggcca cggccgcggc ggtgaacgcg
     2461 gaccgccgta ggaacgtccg acggtcgatc ccgaccggga tccgtcgctc tcggggatgg
     2521 aacgtcacca gggacctccg ctcgattcgc ctatactgac tgaacaatca gttagtagtg
     2581 ccattgcgga ttgtaggaca gaggggaacc ccagggtgag cagcacgtac gacgccatcg
     2641 tcgtgggcgg tgggcacaac ggcctcgtgg ccgcggccta cctggcccgc gccgggatga
     2701 aacccgtggt cctcgaggcc cgggacaacg tgggcggggc ggccacgacc gagacgccgt
     2761 ggggacccga gttcaagatg acggcgctgt cctacgtcat gagcctcatg cccccgacga
     2821 tcctcaacga cctcaagctg gccgagcacg gctaccacgt catccccatg gggccgacgt
     2881 tcatcgccct ccccgacggt cgctacctcc tcgccgagga cgacccggag ctggaccgtg
     2941 acgaggtggc caagttctcc agcaaggacg ccgaccggct cggcgactac cacgcctgga
     3001 tcggtggggt ggccgacgtc ctcgctcccc tcctcctgtc cacgccgccc agcgtgggct
     3061 cccgccgacc caaggacctg atggagcagc tccgcctggc ctggggcctg cgcgggctcg
     3121 acgtgcgggg cacggcggac gccactcgcc tgttcaccat gtcgatccgc gacgtgctcg
     3181 acgagtggtt cgagtcccct gaggtccagg gcctcatggc catcaacgcc gtcatcggca
     3241 cctgggccgg ccccgacgag gcgggcacgg cctacgtgat gatgcaccac accatcggcg
     3301 acgtcggcga cggacatctc ggcaagtggg gctatcccat cggcggtatg ggcgcggtct
     3361 ccgacgccat ccgggcctcg gccgagtctc tcggcgccga gatccgcacc aacgcgccgg
     3421 tcgagcgcat ccggacccgc aacggcagag tcatcggcgt ggccctcgcc gacggcacgg
     3481 agctggaggc cgacatcgtc gtggccgcca cccaccccca gatcaccttc ctgcgccacc
     3541 tggaccgcag cgagctgccc gacgacttcg tccacgacct cgagcactgg cgctcccgct
     3601 ccggcacggt caaggtcaac gtggccctgt ccgagctgcc cgacttcgac gcctggcccg
     3661 gcccgccgga ccaggaaaag ctcagcgggt cggtcgagct gtgccattcg atcgaccacc
     3721 tgcagaaggc cttcgaagag gcgcggtggg gcaaggcctc gaccctgccc ttctccgacg
     3781 gggtcatccc ctcgacggtg gacccgtcgc tgtgccccga gggcacccac gtcatgtccc
     3841 tgttcaccca gtgggtcccc caggagtggt cgagcgagcc ggaccacgcc gaactggagg
     3901 cctacgccga ccgggtgatc gacggctaca acgacctggc ccccaacttc aaggcatcgg
     3961 tcatccaccg gcaggtcatc gggccctacg agatggagaa cgagtacggg ctcatcggcg
     4021 ggaacatctt ccacggcgag ctctcccccg accagctgtt ccacatgcgc ccggcccccg
     4081 gctacgccga cttcaccacc ccgatcgggg gcctgtacca gtgctcctcg gccacccacg
     4141 gcggcggcgg cgtgaccggc atccccggcc tgctctgcac gcgacgcatc ctgaaggacc
     4201 acaagcgcag tcgcctcctg cggcgaggtg agcggtgagc agaatggacc tgcgctctgc
     4261 cgctccggtg gaccaggccg ccatcgaagc gatcctggcg cctggcgccg ccggccggat
     4321 gcttccggcc gaggcctact cctccgaggc cgtgctggcc tgggagcgcc gccacctctt
     4381 cgccgagagc tgggtctgcg ccggccgctc ggctgacctc gccgacgccg gtgaccgccg
     4441 ggccctccgc gtgggcgacg acagcgtcgt gctcgtccgg ggcgacgacg gtgtgctgcg
     4501 cgccttctac aacgtgtgcc gccaccgggg ccaccagctc caacccgagg acaccacggt
     4561 ccggcgcaac gccatccact gcccgtacca cgcctggacc tacgccctcg acggcacgct
     4621 caagttcacc ccccgcttcg acgcgccgga gggcttcgac ccgacccagt tccccctggt
     4681 cccggtccgc accgaagagt ggtgcggctg ggtcttcgtc aacgcctcgg gcgacgcccg
     4741 cccgctggcc gagcacctcg gtgacctcga cgccgagatg gcccgctggg atcccgagcg
     4801 cctcgtggtc ggcgccagcc acagctacga gttggccgcc aactggaagc tgcccatcga
     4861 gaactaccac gagtgcttcc actgcgccgc catccacccc gagttctgcg tggtcagccc
     4921 gcccaacagc ggcgacaact gcgccgatca cgacgggatg tggatcggcg gctggatggt
     4981 gctggccgac gacgccgaga ccatggcctt cgacggccgc tccgagggcg tgatgctccc
     5041 gggcatcgac gagacctggc ggcgccgagt ccactacatc gggctgctgc ccaacctgtt
     5101 gatcagccct caccccgact acgtgatgac ccaccggttc gagcccctgg gccccgaccg
     5161 gaccttcgtc gagtgccagt ggctcttcga ccccgcggcg gtcaaccgcc ctggcttcga
     5221 ccccgcctac gccgtcgacc tctgggacct gaccaaccgc caggactggg ctgcctgcga
     5281 aggggtccag cggggagtgg cgtcacgggg ctaccgtccc gggccgttcg ccgcggacga
     5341 ggacgccgtt acccagttcg tgcaactgat ggcccagacc taccgcgacg gcgccctgcc
     5401 gagcggtgtg cccttcccgg aacaggagct cacccgatga ccgtgaccgc cggcaccgac
     5461 gccgaactgg ccgtcatcga cggcctcatc gccgagcagg aagcggccct gctggaccgc
     5521 accccggcct cggccgcgat catggacgaa gctcgcggcg ccctggccgg cggcgtggcg
     5581 tcgtcctggc aggacgcccc gccccgggcc atctggatcg accatggcaa gggctccaag
     5641 atcaccgacg tcgacggcac gacctacgtc gacctccaca acggcttcgg cgccatgctc
     5701 gtgggccacg cccaccccgc cgtggtcaag gccgtccagg aacgcgtggc cctcggcacc
     5761 cacttcgccc agcccaccga ggacctgatc ccggtcagcc gcgacctggc tcgtcgcttc
     5821 gaccagccca agtggcggtt cgccaactcg ggcaccgagg ccaccctcgc cgccttcaac
     5881 ctcatgcggg tggtgaaggg ccgcaagaag gtgatcaaga tcgagggcag ctaccacggc
     5941 caccacgaca gtgtgcaggt ctcggtgtac cccgacttcg aggacg
//
